LOCUS       DANLXQ010000001        53056 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.001, whole genome shotgun sequence.
ACCESSION   DANLXQ010000001 DANLXQ010000000
VERSION     DANLXQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 53056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53056
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.001"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            46..1653
                     /gene="pckA"
                     /locus_tag="PXD94_000001"
     CDS             46..1653
                     /gene="pckA"
                     /locus_tag="PXD94_000001"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL6284916.1"
                     /translation="MLNRIEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKG
                     VLTNLGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLK
                     TLVTDELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFK
                     PDFVVMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYF
                     LPLKGVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFN
                     YEGGCYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPI
                     YHIDNIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERG
                     ITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDT
                     RGIIDAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDL
                     AGRFVKNFEKYATNEEGKALIAAGPKL"
     gene            1746..2747
                     /gene="bioB"
                     /locus_tag="PXD94_000002"
     CDS             1746..2747
                     /gene="bioB"
                     /locus_tag="PXD94_000002"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL6284917.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEGLFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            2907..3004
                     /gene="ffs"
                     /locus_tag="PXD94_000003"
     ncRNA           2907..3004
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PXD94_000003"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3039..3746
                     /gene="gloB"
                     /locus_tag="PXD94_000004"
     CDS             3039..3746
                     /gene="gloB"
                     /locus_tag="PXD94_000004"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL6284918.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
     gene            3905..4618
                     /gene="uppS"
                     /locus_tag="PXD94_000005"
     CDS             3905..4618
                     /gene="uppS"
                     /locus_tag="PXD94_000005"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL6284919.1"
                     /translation="MNFDPQNMPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSA
                     VSFAAKHHIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKQLHKNNIRLKIIG
                     DKSAFSENLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAV
                     EQITPALFQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDE
                     AFSDAIYAYQQRERRFGGC"
     gene            4642..5511
                     /locus_tag="PXD94_000006"
     CDS             4642..5511
                     /locus_tag="PXD94_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL6284920.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGMVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            5515..6825
                     /gene="rseP"
                     /locus_tag="PXD94_000007"
     CDS             5515..6825
                     /gene="rseP"
                     /locus_tag="PXD94_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL6284921.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNDETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQVGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            6940..9321
                     /gene="bamA"
                     /locus_tag="PXD94_000008"
     CDS             6940..9321
                     /gene="bamA"
                     /locus_tag="PXD94_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL6284922.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTGRYEAKIDTSVSNNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKFAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRIYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTKNGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSMASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPAADYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAVGPQAIYYNTKSGKFD
                     SMNNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWNTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            9401..10192
                     /locus_tag="PXD94_000009"
     CDS             9401..10192
                     /locus_tag="PXD94_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL6284923.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            10192..11217
                     /gene="lpxD"
                     /locus_tag="PXD94_000010"
     CDS             10192..11217
                     /gene="lpxD"
                     /locus_tag="PXD94_000010"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL6284924.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGRFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            11246..11704
                     /gene="fabZ"
                     /locus_tag="PXD94_000011"
     CDS             11246..11704
                     /gene="fabZ"
                     /locus_tag="PXD94_000011"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL6284925.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            11738..12532
                     /gene="lpxA"
                     /locus_tag="PXD94_000012"
     CDS             11738..12532
                     /gene="lpxA"
                     /locus_tag="PXD94_000012"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL6284926.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(12570..14570)
                     /gene="mnmC"
                     /locus_tag="PXD94_000013"
     CDS             complement(12570..14570)
                     /gene="mnmC"
                     /locus_tag="PXD94_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL6284927.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNPEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEPNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYDEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLRRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(14795..16231)
                     /gene="trpCF"
                     /locus_tag="PXD94_000014"
     CDS             complement(14795..16231)
                     /gene="trpCF"
                     /locus_tag="PXD94_000014"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL6284928.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQISADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            16319..16630
                     /locus_tag="PXD94_000015"
     CDS             16319..16630
                     /locus_tag="PXD94_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL6284929.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
     gene            16784..17512
                     /gene="truC"
                     /locus_tag="PXD94_000016"
     CDS             16784..17512
                     /gene="truC"
                     /locus_tag="PXD94_000016"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL6284930.1"
                     /translation="MTLDILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQI
                     GQHVFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVQKTYLAVVRGYLQGSAQID
                     YPLKVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPKTGR
                     KHQLRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQKIE
                     IIASLDEQWQRLFAFFDWNFTQYY"
     gene            17530..17694
                     /locus_tag="PXD94_000017"
     CDS             17530..17694
                     /locus_tag="PXD94_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL6284931.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIANQLRDK
                     YQILEKSNNE"
     gene            17687..18163
                     /locus_tag="PXD94_000018"
     CDS             17687..18163
                     /locus_tag="PXD94_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6284932.1"
                     /translation="MNKLWTVLALTITSHYSFAAAEKIVTKDLPAEGKPAVVLQVFDE
                     SEKIAKPIKGNKLFISKKQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINS
                     SADKKNHTIITEINLANNQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(18200..19051)
                     /locus_tag="PXD94_000019"
     CDS             complement(18200..19051)
                     /locus_tag="PXD94_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL6284933.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            19112..20521
                     /locus_tag="PXD94_000020"
     CDS             19112..20521
                     /locus_tag="PXD94_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL6284934.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSLFHRLFSSNID
                     KLLFIATKADHITSDQVPNLESLMRQLVQEGGRHAEFDGIETGYLAISSIQATDAVKV
                     TQQDKIIKAIRGIRSSDRKQVTLYPGSVPNRIPDKNYWQFNRFEFDQFEPKQLDYSQA
                     IPHFRMDSVLQFLLADMFE"
     gene            20567..20917
                     /locus_tag="PXD94_000021"
     CDS             20567..20917
                     /locus_tag="PXD94_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6284935.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            20910..21233
                     /locus_tag="PXD94_000022"
     CDS             20910..21233
                     /locus_tag="PXD94_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL6284936.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            21341..21820
                     /gene="smpB"
                     /locus_tag="PXD94_000023"
     CDS             21341..21820
                     /gene="smpB"
                     /locus_tag="PXD94_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL6284937.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            22023..23330
                     /gene="serS"
                     /locus_tag="PXD94_000024"
     CDS             22023..23330
                     /gene="serS"
                     /locus_tag="PXD94_000024"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL6284938.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
     gene            complement(23389..23715)
                     /locus_tag="PXD94_000025"
     CDS             complement(23389..23715)
                     /locus_tag="PXD94_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6284939.1"
                     /translation="MVSVDFKNNLLEFIFYGLFSEHTQTNIINGLQFYPKAGVAFRKD
                     LRFRKRAVYQREIMVFYQLDKIDEPKKVTIIAFRLGRMKNPIRLDTVQNRRVVTEIFM
                     NYPEKD"
     gene            complement(23708..23920)
                     /locus_tag="PXD94_000026"
     CDS             complement(23708..23920)
                     /locus_tag="PXD94_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6284940.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            24107..26692
                     /gene="leuS"
                     /locus_tag="PXD94_000027"
     CDS             24107..26692
                     /gene="leuS"
                     /locus_tag="PXD94_000027"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL6284941.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQGRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            26892..27380
                     /locus_tag="PXD94_000028"
     CDS             26892..27380
                     /locus_tag="PXD94_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6284942.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTDSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            27423..28448
                     /gene="holA"
                     /locus_tag="PXD94_000029"
     CDS             27423..28448
                     /gene="holA"
                     /locus_tag="PXD94_000029"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL6284943.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMEKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(28449..28634)
                     /gene="yacG"
                     /locus_tag="PXD94_000030"
     CDS             complement(28449..28634)
                     /gene="yacG"
                     /locus_tag="PXD94_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL6284944.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(28616..29251)
                     /gene="coaE"
                     /locus_tag="PXD94_000031"
     CDS             complement(28616..29251)
                     /gene="coaE"
                     /locus_tag="PXD94_000031"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL6284945.1"
                     /translation="MTYVVGLTGGIGSGKTTVSDLFSALGVEVIDADVVARQVVEPGT
                     PLLAKIVEHFGEEILTAEKALERTKLRQIVFNNEEEKTWLNNLLHPAIREEMVKKLQE
                     STACYVIWVVPLLIENKLTEYCDRILVVDVSPEIQLERATKRDNSKIETIKNIMAAQV
                     SREERLSYADDVIENNLPFEQGLLLIKEQVQALHYKYLALAKKEKECQKLL"
     gene            complement(29287..29943)
                     /locus_tag="PXD94_000032"
     CDS             complement(29287..29943)
                     /locus_tag="PXD94_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL6284946.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(29946..31142)
                     /locus_tag="PXD94_000033"
     CDS             complement(29946..31142)
                     /locus_tag="PXD94_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL6284947.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(31139..31591)
                     /locus_tag="PXD94_000034"
     CDS             complement(31139..31591)
                     /locus_tag="PXD94_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HDL6284948.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDELKFLLNALSPEQSSRQSEIYEEN
                     TAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLRQ"
     gene            complement(31612..32091)
                     /gene="ppdD"
                     /locus_tag="PXD94_000035"
     CDS             complement(31612..32091)
                     /gene="ppdD"
                     /locus_tag="PXD94_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL6284949.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
     gene            32272..33651
                     /gene="radA"
                     /locus_tag="PXD94_000036"
     CDS             32272..33651
                     /gene="radA"
                     /locus_tag="PXD94_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL6284950.1"
                     /translation="MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKE
                     AAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGH
                     PGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANHLGLPTANLKMLSETSVEH
                     ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVG
                     HVTKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGL
                     KEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDH
                     NRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQD
                     LVIFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLS
                     DALDILYNL"
     gene            complement(33706..33936)
                     /locus_tag="PXD94_000037"
     CDS             complement(33706..33936)
                     /locus_tag="PXD94_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL6284951.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTAAHS"
     gene            complement(34056..34550)
                     /gene="folA"
                     /locus_tag="PXD94_000038"
     CDS             complement(34056..34550)
                     /gene="folA"
                     /locus_tag="PXD94_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL6284952.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            34626..34781
                     /locus_tag="PXD94_000039"
     CDS             34626..34781
                     /locus_tag="PXD94_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
                     /protein_id="HDL6284953.1"
                     /translation="MATLFLVTSEQIMSKTIVIKFGTSTLTHGTKSLSRPYMLELVKL
                     NYTNSIV"
     gene            34778..35746
                     /gene="proB"
                     /locus_tag="PXD94_000040"
     CDS             34778..35746
                     /gene="proB"
                     /locus_tag="PXD94_000040"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL6284954.1"
                     /translation="MIVVTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWE
                     NLFDIYNIHIGQMLLTRADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKV
                     GDNDNLSALVAILAQADQLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGG
                     SSTGLGTGGMSTKITAADIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRL
                     EGRKRWLFAAPQVGEIVVDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGK
                     ALALGIARYSSEALLRIKGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            35746..36510
                     /locus_tag="PXD94_000041"
     CDS             35746..36510
                     /locus_tag="PXD94_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL6284955.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            36513..37145
                     /gene="nth"
                     /locus_tag="PXD94_000042"
     CDS             36513..37145
                     /gene="nth"
                     /locus_tag="PXD94_000042"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL6284956.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            37342..38592
                     /locus_tag="PXD94_000043"
     CDS             37342..38592
                     /locus_tag="PXD94_000043"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL6284957.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELKHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(38693..39628)
                     /locus_tag="PXD94_000044"
     CDS             complement(38693..39628)
                     /locus_tag="PXD94_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL6284958.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWDF
                     YLGELLIFLGVIVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(39757..39951)
                     /gene="iscX"
                     /locus_tag="PXD94_000045"
     CDS             complement(39757..39951)
                     /gene="iscX"
                     /locus_tag="PXD94_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL6284959.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(39951..40292)
                     /gene="fdx"
                     /locus_tag="PXD94_000046"
     CDS             complement(39951..40292)
                     /gene="fdx"
                     /locus_tag="PXD94_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL6284960.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(40311..40676)
                     /locus_tag="PXD94_000047"
     CDS             complement(40311..40676)
                     /locus_tag="PXD94_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL6284961.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(40686..42539)
                     /gene="hscA"
                     /locus_tag="PXD94_000048"
     CDS             complement(40686..42539)
                     /gene="hscA"
                     /locus_tag="PXD94_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL6284962.1"
                     /translation="MALLQISEPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVL
                     LDEQERALVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQN
                     LPYQFVASENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYF
                     DDAQRQSTKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSIL
                     RLRKGVFEVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVA
                     LSQAVEFEVKFANWTGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMV
                     GGSTRVPFVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVP
                     LSLGIETMGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSL
                     GKITLRGIPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEV
                     TQMIKSSMANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQL
                     ARLIELKEGTDRFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
     gene            complement(42855..43196)
                     /locus_tag="PXD94_000049"
     CDS             complement(42855..43196)
                     /locus_tag="PXD94_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL6284963.1"
                     /translation="MKAILCQRAKQLRTNMTEAEKVLWQALRAKRFLAIKFKRQQVIG
                     NYIVDFVCFSAKLVIELDGSQHLDQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAV
                     LDTIFYEITRG"
     gene            complement(43344..43976)
                     /locus_tag="PXD94_000050"
     CDS             complement(43344..43976)
                     /locus_tag="PXD94_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL6284964.1"
                     /translation="MKNLEQLLDKQSAWLILRDWFTQAKNHYEILASYPEDAGSALVG
                     MQLSTRSPLGAMVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSH
                     LLVADDAIGGYFAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHF
                     YQDLRWENWQTDVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYSVA"
     gene            complement(44030..44551)
                     /gene="hscB"
                     /locus_tag="PXD94_000051"
     CDS             complement(44030..44551)
                     /gene="hscB"
                     /locus_tag="PXD94_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL6284965.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWHE
                     KLETIEHRKDETELTVFLKQIKAEQKAVLEQLEQTLNTADWQKANALTDKLRYFKKLI
                     IQIEKVEEKFFDY"
     gene            complement(44561..44884)
                     /gene="iscA"
                     /locus_tag="PXD94_000052"
     CDS             complement(44561..44884)
                     /gene="iscA"
                     /locus_tag="PXD94_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL6284966.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            complement(44993..45376)
                     /gene="iscU"
                     /locus_tag="PXD94_000053"
     CDS             complement(44993..45376)
                     /gene="iscU"
                     /locus_tag="PXD94_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL6284967.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            complement(45503..46723)
                     /locus_tag="PXD94_000054"
     CDS             complement(45503..46723)
                     /locus_tag="PXD94_000054"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL6284968.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            complement(46778..47230)
                     /gene="iscR"
                     /locus_tag="PXD94_000055"
     CDS             complement(46778..47230)
                     /gene="iscR"
                     /locus_tag="PXD94_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDL6284969.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISVGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWERLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
     gene            47578..49059
                     /locus_tag="PXD94_000056"
     CDS             47578..49059
                     /locus_tag="PXD94_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL6284970.1"
                     /translation="MKMKNFSKTLIASLVAFSSAGASAAAFQLAEISTSGLGRAYAGN
                     AAVADNASVVATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTA
                     IVPNAYIVSPINERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLSAVNVNFSAAYD
                     LGYGFVLGAGLNAVHAKAELGRFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAG
                     IAAGNTALEAQKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLK
                     GDKWALGWNVGLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAE
                     IDGTLRLRLPAFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTEN
                     FNDSQRIALGLSYDVNEMLTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTP
                     NLSADVGYSHLRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
     gene            49126..49680
                     /locus_tag="PXD94_000057"
     CDS             49126..49680
                     /locus_tag="PXD94_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL6284971.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGLPNKRF
                     LLQLEDITYKDKGIEFVKPKTKKW"
     gene            complement(49776..51626)
                     /locus_tag="PXD94_000058"
     CDS             complement(49776..51626)
                     /locus_tag="PXD94_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL6284972.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTETKPIFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCVVDVAMSGEETFQHFA
                     QNSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
     gene            complement(51681..52685)
                     /locus_tag="PXD94_000059"
     CDS             complement(51681..52685)
                     /locus_tag="PXD94_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6284973.1"
                     /translation="MPGDANKIISNGTSAGGALSTLLGASADHYDYEPYLKEAGALNA
                     SDKIFAVSAYCPITNLENADMAYEWQFNGVNEYSRIDMSRLNAAEFNDRSKPKPKPKI
                     EGSLNEAEIKVSNELAERFPTYLNSLHLVDEKGNPLTLDPKGNGSFKDYLSEVVKTAA
                     NKAYRGLVQDSEEQKAFQQISWLSFEKGKVSSVDWFGYVFSDKRMKSPPAFDALNGSS
                     GENNLFGTDTENNRHFTLYSAERSANKDLNLADPQIVKRMNPMHYLDNPNAAEHWRIR
                     VGTADRDTSLAISAILAIKLQMAGKNVNYETPWNVPHSGDYDVNELFLWADELVKGKK
                     "
BASE COUNT        16082 a        11176 c        10319 g        15479 t
ORIGIN      
        1 taatcaaccc agttttaaat ttataactat tatgaggtaa ccactatgtt aaatcgtatt
       61 gaacaagaac ttgcagactt aggcattaca aatgtaaaag aaatcgtgcg taaccctagc
      121 tatgagcaac tgtttgaaga agagatgaaa tcggatctta aaggttatga aaaaggcgtc
      181 ctaacaaatt taggcgcagt agcggtagat accggcattt tcacggggcg ttcgccgaaa
      241 gataaatata tcgtattaga tgaaacgaca aaagataccg tctggtggac atctgatgca
      301 gctaaaaatg ataataaacc gatgactcaa tctacttggc aaagtttaaa aaccttagtg
      361 accgatgagt tatctaataa gcgtttattt attattgatg ctttctgtgg tgcaagccct
      421 gagcatcgca tcaatgtgcg tattattacc caagttgcgt ggcaagctca ctttgtgaaa
      481 aatatgttta ttcgtccaac agaagaagaa ttaaaagatt tcaaaccgga ttttgtggta
      541 atgaacggtt cccaagtaac taacccgaac tggaaagagc aaggcttaaa ttcagaaaac
      601 tttgtggcat ttaatatcac agaaggtgtt cagatcattg gcggtacttg gtacggtggt
      661 gagatgaaaa aaggtatgtt ctctatgatg aactacttct taccattaaa aggtgtcgct
      721 tcaatgcact gttcagccaa tgtgggcgaa gaaggtgatg tggcggtttt ctttggttta
      781 tcaggaaccg gtaaaacgac actatcaacc gatccgaaac gcaaattgat tggtgacgat
      841 gaacacggtt gggacgaaaa cggcgtattt aactatgaag gtggttgcta tgcgaaaacc
      901 atcaaccttt ctattgaaaa cgagccggat atttatcgtg caatccgccg tgatgcgtta
      961 ttagaaaacg tggttgtgcg tgaagatggc actattgatt ttgatgataa atcaaaaaca
     1021 gaaaataccc gtgtgagcta tccgatttat catattgata atattgccgt gccgtcaaaa
     1081 gcaggacacg ctaaaaaagt gattttctta acggcagatg cattcggcgt attgccaccg
     1141 gtgtctaaat taacgcctga acaaactaaa tactacttct tatcaggctt taccgcaaaa
     1201 ttagcaggta cagagcgtgg tattactgag ccaaccccaa ctttctctgc ttgtttcggt
     1261 gcggcgttct tatcactcca cccgacacaa tatgctgaag tgttagtgaa acgtatggaa
     1321 gcagccggtg cggaagccta tttagtgaat accggttgga acggcacagg taaacgtatt
     1381 tcgattaaag atacacgggg tattattgat gccatcttag acggctcaat tgagaaagcg
     1441 gaaatgagta cattaccaat cttcaattta gcaattccaa ctgcgttacc gggtgtagaa
     1501 acccacattt tagaccctcg taacacttat gaagacaaag cacaatggca agctaaagca
     1561 gaagatttag ctggcagatt tgtgaaaaac tttgaaaaat atgcgaccaa cgaggaaggc
     1621 aaagcattaa ttgctgctgg tccgaagtta taagttgtaa ttataagtta taatcgctaa
     1681 aaaattagtt taggactaaa ccttgaatga ggtttagtcc ttttgtataa aggagaagta
     1741 caataatgac aacattccaa ttaaggctga aagccaaaga tgagcttacc ccacacccaa
     1801 cagcacaata ttggcgaaaa tgccaagtgg aaggactatt tgatctgccg tttatggatt
     1861 tagtgtttag agcggcacaa gttcaccgtg aaaattttga tgctaatgcg atccagcttt
     1921 caacgttact ctcaattaaa acaggaggct gtccggaaga ttgcgaatat tgcccccaat
     1981 ctgcccgcta tgatacggga atagaaaagc aaacaatgct tgatgtagag acgattgtag
     2041 aaaaagctaa aattgccaaa gagcgtggag caagccgctt ttgtatgggg gctgcgtggc
     2101 gaggacctag gcctaaagat attaaagtag tctcagaaat catcaatgcg gtgaaatcat
     2161 tggggctaga aacctgtggt actttcggta tgcttgaaga gggaatggca gaagaattaa
     2221 agcaagcagg gctggattac tacaaccata atttagatac cgaccctgaa cgctataaca
     2281 aaattatcca tacccgaaat cacgaagatc gaatggacac tttaggtaaa gttcgcaatg
     2341 ccggcttgaa agtttgctgt ggcggtattg tgggaatgaa cgaaacccgc cccgagcgag
     2401 caggcctaat tgcaagcctc gctaacctcg atcctcaacc tgaaagcgta ccgattaatc
     2461 agttgattaa agtagaaggt acaccgctta ccaatgccga agatttagac tggacagaat
     2521 ttgttcgtac cattgcggta gcaagaatta ctatgcctca aagctttgtc cgactttctg
     2581 ctggcagaac tactatgccg gaggcaatgc aagccctctg ttttatggca ggagcaaatt
     2641 ctattttcta tggcgataaa ttacttacta cagaaaatcc ggaagaagat catgatcgcc
     2701 aattaatgga taaattagat ttatatccgc tacaatatat agcctgagtg ccaatgattt
     2761 tcgcctgaat gaaaattaaa gaatcaatcg ttattttacg caaatttcta acataaaata
     2821 accgcttgca aaattcgttc ttgtcttatt tttaaattta tctataatgt gaggccttac
     2881 gcaagcttgc gtaattcgat ggggcgttat tggttcctcg caatggtgtt cagcttactt
     2941 gatcaggttc ggaagaaagc agtctgggtt gaaatttccg tgtgccgagg gaggctggta
     3001 atgccccacc cttttttcag ttctatcagg agaacgttat gctccagatc acccccattc
     3061 cggcattaaa agataattac atttgggcaa tccgtaacgg gcaagatgtg attattattg
     3121 atccatcaga acatcagcca gcattaaact ttattgcaaa aaatgcgtta aatttaaccg
     3181 ctatcttgat tacccataat caccacgacc atactgatgg ggtgagtggt attgttgagc
     3241 aatatccgaa tatccctgtt tatggtccgc aagaagtggc tgaatttgcc aatgtgattg
     3301 tctcgcctga acaacatatc agcctgtttg gttatgatgt aaggattatt gaaagtggtg
     3361 ggcataccgc agggcatatc agttatttat ttggctatga ttatctgttt tgtggcgatg
     3421 cgttattctc cggcggttgc gggcgggttt tcaccggtga ttaccaagcc caatttgatg
     3481 cgttacaacg ctttaaaaat ctgcctaact cggttaaagc ctacccggct cacgaataca
     3541 ccaagagcaa tttgaaattt gcagaagcgg ttctaccacc aagctgtgta ttatcggaat
     3601 atcaagaaca ggttgatctt ttacgttcgc aaaacaaacc gactttgcca accactattg
     3661 gcttggaaat gcaggtgaac ccgtttatgc aagcggtcga tttagccgaa tttattgcac
     3721 ttcgtcagca aaaagacaat ttttaacctc atatttctaa ttaagcatta aagcctctca
     3781 ctttaatgct ttttggttaa ataaaacctt tgcaaacatc gcttgtcaat tccactctgg
     3841 cttatcctat ggtatagtta cgactatttt tagcttaatt tattatttta gtgagtaaaa
     3901 ttctgtgaac ttcgatcctc aaaatatgcc taagcacgtt gccattatta tggatggcaa
     3961 tggcagatgg gctaagcaga aaggaaaatt acgcattttt ggtcatcaga atggtgtaaa
     4021 ggcagttcgt tctgcagtta gttttgcagc aaagcatcat atccaagttt taaccctcta
     4081 cgcatttagc agtgagaatt ggagcagacc agaaactgaa gtttcagctt taatgtctct
     4141 gtttatgaaa gctttagatt ctgaagttaa acaattacat aaaaataata ttcgcttaaa
     4201 gattataggc gataaatccg cctttagcga aaatttacaa aaacgcattg ccgaatcgga
     4261 aaaattaacc gaaaacaata ctgggctaat cttaaacatt gctgccaact atggtggtta
     4321 ctgggatatt gttcaagctg ctcaacaact agcagtgcac gttaaatctg aacaacttgc
     4381 tgtggagcag attacaccag cattgtttca acaacattta gtgacagaaa accagccgca
     4441 agtggattta ttaattcgaa ccagtggtga gcagcggatc agcaattttc tattatggca
     4501 aattgcttat gccgagctgt tttttagccc ggttttatgg cctgatttta atgatgaggc
     4561 ttttagtgat gcaatttatg cttatcagca acgtgaacgc cgattcggtg gttgttaata
     4621 taaaaagaat aaggaaatac aatgcttaaa gaacgtgtac tttcagcgat tattatgatt
     4681 attgccgtgc tggctgccat attttggctc tcaccacttc ctttaacttt agtgttatcg
     4741 gcaattgttg tggctgcaat gtgggaatgg gctcaatttg ccggctttaa acaccctgtg
     4801 ccacgtgcaa ttgtggcaat ggtgacaatc tgcttattac tttttgcgat cgttgcgaat
     4861 actgattata tccgagccac ccgttttatt actgatgaga ctacccctat cctctttatt
     4921 ggctgtattt ggtggttaat tgcccttgtc ctagtggcaa cttaccccaa ctcggcaaaa
     4981 ctgtgggaaa aatctgttgc agctaaattc ctatttggac ttgctacctt agtgccgttt
     5041 ttaattggca cgctcgcatt gcgtttccat aattatgcat ttgatccata ccaaggaact
     5101 tatttattcc tttacgtctg tttattagtt tggggagctg attcaggtgc ttattttgct
     5161 ggcagagcct ttggcaaaag aaaacttgcc ccaaaagtat ctccgggtaa atcgtgggaa
     5221 ggcgtaattg gtggcgtact cacctctagt ttgattgcct ttaccttttt acaacttaca
     5281 ccggcaaatg tattcggcaa agcattagcc actaccccat ttattttagt ttcggtcggt
     5341 atggttgcta tttcagtctt aggcgatttg gcagaaagta tgtttaaacg ccaagcaggc
     5401 attaaagaca gtagccagtt aattcctggg catggaggta ttttagatcg tattgatagt
     5461 ttaaccgctg ccgttccgtt ttttgctaca ttcttctttt ttgtactata gaatatgacg
     5521 tccatcattg cattttttat tttaatttgc gtactggttt ttgtgcatga atacgggcac
     5581 ttttgggctg ctcgcaaatg cggggtaaag gtgatccgtt tttcgatcgg atttggtaaa
     5641 gtcttattta agaaaaaaga taaacaagga accgaatttg ttttttcact tattcctctg
     5701 ggcggctatg tacaaatgtg gaatgatgaa acggatatca acgctccagc tcaacaagct
     5761 cttaacacaa aatctgtatt gcaacgtgca tttattatta tagccggtcc tgctgcaaat
     5821 tttatttttg cgattttggc atactgggtc gtttttattg ccggtattcc aactgttaaa
     5881 cctgttattg gggaaatttt accaaacact atcgcctccc aagcaagaat tcctaccgaa
     5941 ttggaaatta caaaaatcgg tcatcaatct atccaagatt gggaaagtgc atctttagca
     6001 ctagttggtt ttgtgggcaa taaaaatgta ccgttagagg gaaatatcaa cggcaactca
     6061 acacaaaaat atgaactcga tttatctaac tggagtattg actcagccaa agaaaaccca
     6121 ttaaccgcat taggtattcg ccccaaaagt gcaaaagtca ccgctgaaat taaacaagtt
     6181 gtcgaaaatt ctcctgcaag ccaagtgggt attcaagcag gtgatcgtat tcttcaaatt
     6241 aaccagcagc catttaattg gttcaattta gttgaactgg tacaagcagg taaacctatt
     6301 gaacttaaaa ttgaacaacg aggtcagatt aaagaccttg tcgtccaacc agaaaaaaaa
     6361 gacgaacgct atattatcgg ggtaatacca agttatgagc ctcttgcaga taaatatcgt
     6421 accgaattaa aatatgatat tcttaccgca ttttataaaa gtatcgaaaa agtgtggtct
     6481 cttacccaaa ccatactcca atttatcggt aatttaataa gcggtgattt gtccattaaa
     6541 aatttaggtg ggccaatttc aatggcaaaa ggagccggtg caacagctga aatcggctta
     6601 gtttattacc ttagttttat ggctctaatc agtgtcaatt tgggggtaat gaacttattt
     6661 ccacttttac ccttagatgg tgggcaactt gttttattgg ctgttgaagc aattcgtgga
     6721 aaagcattat cagaaaaaat acaactcaaa ttccaacaaa ttggatttgc attcgtgctg
     6781 agtttaatgt tattcgcctt tgccaacgat ataattcact tttaacaagc ggttaaaaaa
     6841 ggtgactttt ttgcaaaaag caccctgaca tcaaaatcca taatatggat ttaaactata
     6901 ttcttaataa ttttgcaaaa cgatcatagg ataatttcaa tgaaaaaatt attactttcc
     6961 tctcttttaa ttgcaaacgg tgtagttgct gcacctttcg tagtaaaaga cattcgtgtt
     7021 gaaggtgtgc aacctacaac aggcgaatct atcatttcat ctattccggt tagagtaggt
     7081 caaacagcaa cggatactga cgtatctaat gtcgtccgcc atctattcag ccaacaacgt
     7141 tttgcagatg ttcgtgcaac acgagaaggc aatacgcttg tcatcaaagt cgctgaaaaa
     7201 ccgattattg gcaaggtaga gatcgaaggt aaccaagcga ttcctaaaga tgccttagag
     7261 caaaatttaa aggcaaactt aattaaccaa ggtgaaattt ttgatgctgc caagcttgaa
     7321 gcttttaaag aaggactaat cgaacattat cactctaccg gccgttatga agccaaaatt
     7381 gacacatctg tttcgaataa caccgaaggt ggcgttaata ttaagttagc gattaatgaa
     7441 ggtgaagttg ctaaagccaa aacaattaag tttgaaggta ataatgcctt ctctgataaa
     7501 aaattattag aaaacttaga tattcaacct gatgtttctt ggtggaatat ttttgaaagt
     7561 agcaaattcg aacaaaccgc ctatcaaaaa gacttagaaa cattaagaga tttttattta
     7621 aatcgtggct atgcaaaatt tgctatccaa gataccgatg ttcaattcaa tgataataaa
     7681 acggaagttg cattgaccta taaattagat gaagggtcac agtaccatgt cagtgaaatg
     7741 cgcattatcg gtaatacagc caatatggat agccagctca acgccctact aaaagaattc
     7801 aagcctggtc aattattccg taaatcagac cttacacaaa ttgaagaaga gattaaatct
     7861 attttagggg acgcaggtta tggttcagct aaagtagata tttatcctca atttaatgaa
     7921 gaaaataaaa ccgtaaaaat cagttttgtt gtggatgccg gtcgtcgtat ttatgttcgt
     7981 aaaatccgtt ttgaaggtaa tggtgtcact gcagattcaa cattacgtcg tgaaatgcgc
     8041 caacaagaag gtgcttggct ttccacaaac aaagcttctc taggtaaagc tcgtttagaa
     8101 agaaccggtt tctatgaaag tgttgatatg tcaatgccaa atgtagaagg tacagccgat
     8161 caagttgatg tagtgtataa aattaaagaa cgcaataccg gtagtattaa ctttggtatc
     8221 gggtacggca cgaaaaacgg tatcagctat caagcaggaa ttaaacaaga taatttctta
     8281 ggtatggggt caactatcag tctaaatggt acccgtaata actacggtac cagcgttaac
     8341 ctaggctata ccgaacctta ctttactaaa gatggtgtaa gtttaggcgg taatattttc
     8401 tatgaagatt atgataactc agacaatgat tcaatggctt catataaacg ccaaacctat
     8461 ggtattaacg gcacattagg cttcccggta aatgaaaata actcatacta cttaggttta
     8521 ggctatacgc acgataaaat taaaaatgca gcacgtgagt acactcgtga gaaatatgta
     8581 aaatcaatga actttgagtt cgatccagct gccgattatt acaaaaaaat caaagctgat
     8641 gattttgatt tttctctcgg ttggaactac aacagcttga atagaggcta tttcccgact
     8701 gaaggaacta ccgcaaacat tggcggtaaa atcacaattc caggctctga taataaatat
     8761 taccgagtca gtgcagactt tagaaattat tacccgttaa accgtgagca caaatgggta
     8821 atttcaacga aagctagcat tgcttatact aatggatttg gtggtaaaga agttccgttc
     8881 tatcagcttt atactgccgg aggtattggt acactaagag gtttctctta cggtgcagta
     8941 gggcctcaag cgatttacta taatacaaaa tccggcaaat ttgatagcat gaataatgat
     9001 attatcggtg gtaatgcgtt agctgcggca agcttggaat taatcacacc aacgccattt
     9061 gtgagcgaaa aatatcagca caatgtaaga acctctctct ttgttgatgc tgcaagtgca
     9121 tggaatacaa aatggaagaa agatgaatat acgaacttgc ctgactatgg tgatttcaaa
     9181 cgtttccgtg cttccgccgg tattgcattc caatggatgt ctccaatcgg gccattatca
     9241 ttctcttatg cgaaaccaat tcgtaaatat gataatgatg atattgaaca attccagttc
     9301 aacattggta gttcattcta attaaccaca agcggttaat aaatgtaaaa aatttgcaac
     9361 ttagccgctt atttcaaaac tgttcataca aggaaaacaa atgaaaaaat tatttaaaat
     9421 gactggctta gccgtagctt tagcctctac aaccggaatc gcgaatgctg cagatactat
     9481 tggtttcgta gatccaagct atgtattaca aaaccaccct gtattattag atgcttcggc
     9541 aaaatttgat aaattcatga aagaaagcca agaaaaattt gctgctgatg aaaaaaaatt
     9601 agcagatgaa aataaaacct taacggctga gcgcaataaa attaatgaag atgcgcaaaa
     9661 attacaaaat gaacaaaaaa atgtagaagc ttctatcaag aaaaaagtgg cagcattaga
     9721 gaaagatgcg cctcgcttac gttcaaaaga gattcaagca cgtcaaaatg cgattcaaaa
     9781 agaagcaaac gcattccaag ctaaagtaac agcaattcaa aaacgtgaag ctgattttgc
     9841 aaaaaaagca gaagccttcc agaaacgtgc agatgaattc caaaaacgta ttgaacaagc
     9901 gaataaagaa aatggtggca ttaacccaca agaagcgcaa aaacaagcgg ttgatgaagt
     9961 aaatgcaacc attaaagaga tcgcaaaatc gaaaggttac actttagtat tacaaccaca
    10021 agtagcactt tatgctgaag atgaaggtaa agatattact gaagcagtac ttgatgctat
    10081 tgttaaaaaa catccggaaa ttaaaataga aaaacctgca actgaagctc aaccagctac
    10141 agaaaaagaa accaaagctg aagaaactaa accagaagaa gcgaaaaaat aatggcaaat
    10201 ttccgtttaa ttgatttagc ggagcatatc ggcggtactc ttaggggtaa cgccgatttg
    10261 gctattcaaa gtattgctgc tttagataaa gccgacagct cgcaaattac ctttatctca
    10321 aatgcaaaat atcgggaaaa tttgaccgct tgcaatgctg gagctattat cgtcagcccg
    10381 gcagatgtag aattttgccg agcagatcaa aatcttatca tcgttgccaa tccgtatgtt
    10441 gcttatgctc aactggctca atatatggat tctactccga aagcagcgag cgaaatccac
    10501 ccaagtgcgg tgatttcacc tgaagctaaa ctgggtaata acgtatcggt aggggcaaat
    10561 gccgtgattg aaagtggcgt agaattgggc gatgatgccg tgatcggtgc aggctgtttt
    10621 gtgggaaaaa acagtaaaat cggggcaaga acccaacttt gggcaaatgt ttcggtgtac
    10681 cacaatgtac aaatcggagc tgattgcttg attcaatcct caacggttat cgggagtgat
    10741 gggtttggct atgcgaatga caaagggcaa tggattaaaa tcccacaaac cggtggtgtg
    10801 attatcggta atcgtgttga aattggtgct tgcacttgta ttgaccgagg tgcattagat
    10861 ccaaccgtaa tcgaagataa tgtgattatt gataaccttt gccaaatcgc ccataatgta
    10921 cacattggtt ttggcactgc cgttgccggc ggcgtcatta tggcaggcag tctaaaagtt
    10981 ggtcgctttt gccaaattgg tggagcaagt gttatcaacg gtcatatgga aatctgtgat
    11041 ggtgccataa ttaccggaat gggtatggtt atgcgcccaa ttacagaaaa aggaatttac
    11101 tcatcaggta ttccattaca aaccaataaa gaatggcgaa aaaccgcagc acttgtaatg
    11161 aatattgatg aaatgaacaa acgcttaaaa tcgttagaaa aacgttttaa cgattaattt
    11221 tattagaggt cttaaagtga cagaaatgac aactgaaaat cgtgagccaa atattattga
    11281 agtaaccgaa attatggata tgttaccgca tcgctaccct ttcctgttag ttgatcgcgt
    11341 aaccgattat gaagaaggga aatggttaaa agccattaaa aatgtaacct tcaatgaacc
    11401 ttgttttacc gggcattttc ctggcgcacc aatcttccca ggagtattga ttctggaagc
    11461 aatggctcaa gcaactggcg tattagccgt tgccacttat ggtaagttaa gtgaagacga
    11521 actctactat tttgccgcaa ttgacaatgc tcgctttaag cgaccggttg tgccgggcga
    11581 tcaacttcaa cttgaggttg aatttcttaa agaagtacgt ggtattacta aatttaccgg
    11641 taaagcatac gttgatggca aattagtctg tgaagcagat ttaatgtgcg ctcgccgtaa
    11701 ataattcgat ataagcccaa actagaggga acaaactatg cgtctaattg attcaacagc
    11761 aaaaattagc ccgcttgcca tcattgaaga aggtgctgaa attggcgctc acgttgaaat
    11821 tggtccattt tcagtcatcg gtaaagatgt caaaatcggg gctagaacga aaattcactc
    11881 aaatgttgtc attaacggca tgactgaaat tggtgaagat aaccatattt tccaatttgc
    11941 cagcattggt gaaatcaacc aagatctaaa ataccaaggt gaaccaacca aagtggtgat
    12001 cggtaatcgt aaccgtattc gtgaaagtgt tactattcac cgaggtacgg tacaaggcgg
    12061 tggcgtgacg aaaatcggtg atgataactt atttatgatc aacacccaca tcgcacacga
    12121 ctgctctatc ggtaaccgtt gtattatcgc caataatggt acgctagcag gtcacgttac
    12181 cctagatgat ttcgtgattg tgggaggaat gtccgccatt caccaattcg ttgtgattgg
    12241 ttcacacgtt atgcttggag gcggttcaat ggtcagccaa gacgtaccgc cttatgtcat
    12301 ggcacaaggt aaccacgcac gcccatttgg tattaacttt gaagggttaa aacgtcgtgg
    12361 ctttgataaa ccaactatgc acgctattcg caaagtctat aaactgattt actccagcgg
    12421 aaaaacattg gaagagtgcc tagtagaaat tgagcaaatt gcagcaacag aacctgcgat
    12481 tgctattttt aaacaattct tcaaacgttc tacccgtggt attattcgtt aataaacgaa
    12541 accccgaaat caattcgggg tttgttttat caagcgaccg tcgaaccttt caacaatttt
    12601 cgaatccaag ctcgatttgg gctaaggtta tgccaaatat cctcactgat tggcacaggc
    12661 tcattcacaa tcaggcttgc taacaattcc cctagaagtg gtgcagtcgt caaacctcga
    12721 gaagacaggc cgtttaccat atacagattg gtaaaaattt cagcattttc taccgcttgt
    12781 ttccgtctca actggttgta taaattggca tattggcttt tttgctgctc aaaatgatgc
    12841 actgccccaa ccatcggtac acgatcacga aacgccgcac gaataccgac tttggcttta
    12901 ttttcagata aatcaattcc ttgcgtccac tcacaaacgg ttacattttg ctgaagtttt
    12961 gcaacattct cccaatgctc cgataaacta aactcggttt ctgcattatc ccgaatatgg
    13021 cttgctccaa tgcaatgcgt gccataggct gacattggcg ttaaataacc atcataacaa
    13081 agcacacact tcagtttttg taattcgctt gtagtcggaa tttggctcac ttgccctcgt
    13141 accggataca acggaatgcc gttcgcttgg ctaaattcat tcaacgtatg tccatttgcc
    13201 agcacaaccg cttgatgtcg aaccgtttct cccctcgtct gccattgcca cattccatta
    13261 gaaaaggtta aatgctcaac tttgtgattc agtacaatct tcaacccttt ctgctccagc
    13321 caagtaaagc catttctaac aagttgtacc ggtgaaagcc aaccagcttg cggaataaat
    13381 gccccgctgt ttggcacgct taaacctaat ttttcactta attctgcctg tgaacaagat
    13441 tttaataagg tgtcatcagc cgtctgctcc accatttttt ccagtttttt agccgttttc
    13501 tcgtcatagg cgtaaagcac taccccgctt aaattatgct caaactcaat gttggatttc
    13561 agttgttgca agcgttgcaa tgcataatca aaactatgga tataaaactg tacatttcgc
    13621 tcgtcatcat cgcttaactg tgggtaaatc gccccttgta aatttcctga agcatttatt
    13681 gccaaggttt catctttgca atacagcgtg acttgcttgc ctttttctaa cagcgataac
    13741 gcaacgaaga aactcgctac tccgccacca ataattgcaa tgtcatcagc ggtttcattt
    13801 ttggcataaa aataggggta attaggctcg gaaacttggc tatctaacgg tttctcgccc
    13861 caaagcattt ctcgtttttt gccaaatcct ttgcgttttt tcactgcaaa acctgcggct
    13921 tccagccctc gtctgaccat acttgaagcg gtaaaagtag caaaactgcc gccattggca
    13981 cttagtcgaa acatttgcag atataactgt tcgttccaca tttctgggtt tttatcaggc
    14041 gaaaagccat ctaaaaacca agcgtcaatc gtgccgctat aaagatcgcc taattggggt
    14101 aagttttcag caatctcacc gaaccataaa tctaaataaa cctgctcaaa atgataacgt
    14161 tggcaaccta tttggcgagg ctgccaacaa gcggttaaat tttcagataa tgttgcaaat
    14221 tgcggatagc gttggtgaat atcagctaat tggctggccg tcagaggaaa tttctcaaat
    14281 gagataaaat agagtcgttt tagtttactt tgcggaaatg ctgcccgaaa ctgttggaat
    14341 ttgtcagcaa cggctaaaaa attcaagccc gtgccaaagc cggtttccgc aataacaaaa
    14401 gcctctttcg ggtgagattg ccacttttcc cacagttggt taccttcttg aaacacatag
    14461 taactttccg ccaaaccatc ttgggtagaa aaataaatat catcaaattg ctccgacacc
    14521 ggtgtatctg catcattaaa tgtaagtgtc gcaaaagtca gtttacccat catatcccct
    14581 taaaaacaaa acacggaaaa ccccaagatt ttccgtgttt caaccagttc aatagtacca
    14641 atgttagaaa atgttgctgg caaaaataac gatcatacca agtgcgccta caaccgttag
    14701 cattgaataa ccaaaagtac ccattttaca atcctcattg ttaattatga ttaaaacggc
    14761 tttattctag cataatttcc atcgctttga atgcctatgc ttgaataatt ttgccaaaac
    14821 aagcggtcaa tttttgtaaa tcttttacgc ctttggcact ttccacgcca gaatttaaat
    14881 ctaacccgac acagccttga gcaagggctt gctcaatatt ctcagtgcca ataccacctg
    14941 ccaataacgc tttttgtttt aaggtttctg gaatcaacgc ccaattaaag gttttgcctg
    15001 tgccgccttg ctgattgcct actttgctgt ctaatacata acgagcaatc tgtggattat
    15061 cgttaaattc tagattttca acctcaacat caaccgataa ggctttccag atttgagttt
    15121 tactacctaa tttctctgcc agttctgcaa tgtattgctc atcttccgag ccgtgcaatt
    15181 gaactgcaaa taactcaagc tgttttgcca ttttttccac aaattcgacc gcttgatttt
    15241 ggaacacccc cacataacgc aacggtgcat tcaccaccaa ttcttgggct tggcgaagtg
    15301 aaagctggcg aggcgagcct tcggcaaaaa ttaaaccgcc atataatgcc ccttgctcat
    15361 aaaccgcttt cacatcttgc acacgggtta agccacacac tttgttttca ccaaaaatca
    15421 ccgcccgcac cgcattattg agatcggcac ttcccattaa actgctgccg attaaaaaag
    15481 catgaacata cggcttaatg tttttcactt gctggtgatc gttaataccc gattctgaaa
    15541 tcaaacagac atcagccgaa atttgggctt ggtatttttc caccaaacgc acaatgcggt
    15601 tcatatcaat cgtgagcgtg tgcaaatcac ggttattaac gccaattacc ttcgccccta
    15661 aggctaaggc acgttcaaat tccgcctccg tgctggtttc agtcaaaacg cccattccta
    15721 actcgtgagc cagtgtggca agttcacggt aggtttcatc atttaccacc gataacatca
    15781 ataaaatcgc atcagcatta taaaaacggg caaggtaaac ctggtatgcc gaaatcataa
    15841 aatctttaca caaaatcggc tgtgtggttt ggcgtttgac ttgatcgata taacggaaat
    15901 cgccttgaaa atattgctca tcggttaaaa ccgaaatcgc cgcagcgtaa tttttataca
    15961 cttgggcaat actgtctaaa tcgaactcag cacgaattaa cccttttgac ggtgaagctt
    16021 ttttacattc taaaatataa gctggttctt ggtgtgtgcc tttggcaaga gccgcataga
    16081 aatcatggtc gctttttaca attttgtgct gaaattcact taacggaaag gcttgctctt
    16141 tttgtgccac ccaaatggct ttatcttgta cgatcttttg taaaatcgtc ggttggtttg
    16201 gttgagcgtt atcttgaacg atgttttgca tataaattcc tagagcgaaa tcggtttgaa
    16261 tggtaaaatc tgcccttatt tttacccgaa aaagtctcat ttcacaagca aaactaagat
    16321 gaaatcacaa gttatacagc atttatctta tttagaaatc gccctgagag ttaatcaact
    16381 ttgggaggcg acaccaccaa gccctgaagc cttagccaat gagcaacctt tctgcgtgga
    16441 aaccttaacc ccaacccaat ggctacaatg gatttttatt cccagaatgc gggccatttt
    16501 agatgcaaat gccgatttac ctcgcaattt tgcaatcacc ccttatttag aagaggcatt
    16561 gaaaaacgaa ggttatttac aagcgatttg tgaaccgcta ttaggtttgg aaaagttgtt
    16621 gaaagattaa cttctctctc agttctttta ttaatctgac aaattcgttg atagtgccag
    16681 cgtccacgct ggtactaatt ttaacggtta attaatcaaa atttggcaca agcgaggacg
    16741 cttgcgccat catagagatt tgataaatta tacttaatgt attatgacct tagatatttt
    16801 ataccaagac aactcgctta tcgccatcaa caaacctgcc ggagtgttgg tgcatcgttc
    16861 ttggttagat aaacacgaaa cggtgtttgc aatgcaaacc ctgagggatc aaatcgggca
    16921 acacgttttt ccgattcacc gccttgaccg cccaacctca ggtgtattgc tgtttgctct
    16981 caacagcgaa accgcccgaa taatgagcga gcagtttgaa caacacctag tacaaaaaac
    17041 ttatttggcg gtggtgcgtg gctatttaca aggttcggca caaattgatt atccgctcaa
    17101 agtgatttta gataaaattg cggataaatt ttctcaggaa aaagaggctc aagcggcgat
    17161 tactgattat aaaggcttag caacggttga aatgccttac cctgtcggca agcaccaaac
    17221 ctctcgttat agtttggttg aacttagccc taaaaccggg cggaaacatc agcttcgccg
    17281 ccacctgaaa cacctgttcc acccgattat gggagacagc aaatatggcg acttacacca
    17341 aaaccgtgct ttagttgaaa aatcaggcgt aagtcgttta atgttacacg cccataaatt
    17401 gcaatttcag cacccgaaaa atttgcaaaa aatagagata attgcatcgc ttgatgagca
    17461 atggcaacgc ctctttgcat tttttgactg gaactttact caatattatt agacaaaaaa
    17521 gaggattgaa tggataattc acttttatca ttgacacacg agcaacaaca agccgcagta
    17581 gaagaaattc aaaaactgat ggcacaaggc atcagtgccg gagaggcaat tcaaattatt
    17641 gctaatcaat tacgtgacaa atatcaaata ttggagaaat caaacaatga ataaattatg
    17701 gacagtgctt gccctaacaa taacttccca ctatagcttt gctgccgctg aaaaaatagt
    17761 cactaaagat ttaccggcag aaggaaaacc tgcagtcgtg ctacaggttt ttgatgaaag
    17821 tgaaaaaatt gcaaaaccta ttaagggaaa taagctcttt attagcaaaa aacaaacaag
    17881 gctttgctgg cacagtatta atcttcctgt acaaaataaa gttactattg cagaagcttt
    17941 ctatgcccct gctgcaatga aattagcttc tcccggctct aaaattaatt cttctgcaga
    18001 caagaaaaat catactatca ttactgaaat aaaccttgca aataatcaag tattaaatcg
    18061 ttgctggggt tttgataaaa ctgaccctgt cggtaaatac aaaatggaaa ttcaaattaa
    18121 cgatcacatt ttcaaaggtt tagaatttga aattgtaaaa taacctaaaa gccacagagt
    18181 aacaccctgt ggctttttgc taaatcattg tttttaaatt ggttaaattg tgcagcaaat
    18241 aatctttact cttcacaaag gctctcgcct gttgccagtt gctaaattgt tgctgagtgc
    18301 attgattaaa acgtaccagc acttccaaac tttcattgcc gacacaaata aaatcagcta
    18361 ataattgggt taatttttca ctgttttcac catccattgc agttgcaatt ttctgctccc
    18421 atttaaccgc ttgtccgagc aaataacccc gctgggcttt gcttaaatta ctcaaccaca
    18481 tcccatccgc ttttggcatt gcctcaacca aagtaaataa gtcgcctaga tttccttgct
    18541 tgatttcaaa ctccaattca caaatcggct cttcaccaaa atgattttta atgatgccct
    18601 gatctaacgc tacctcaatg tcagacatcc ccacttgcac caaccaagtt ttacgagtga
    18661 aatcggtgct gaatatcgct tgcaagcttt ctgcaatctg ctctgaattt tcaaacggca
    18721 actggtattt ttccactaat gtttgaaaat ccggctggct attgggtaaa gctaaattat
    18781 attccggacg aatatgcaaa ccgcctacaa tttccccttg tgtttttaac gtgatttcat
    18841 attgttgatt cacagaacgc acccgcagcc ccattttttg cgtggcaaaa aattgctcgg
    18901 gcgtatcata ataggtgttg cctaacaatt ccgtttgatg agcaagcaca gggaatggcg
    18961 aggtttgatt caagtagttt tccactaacg ccacattctg ctgttccaac attattttca
    19021 gttcgatttc tgcttgtttt tggttttcca tttcacacgc ccctgcaacg ttgtataatc
    19081 aagcggttat tttatcaata aattttgcaa aatgttttca aaaattcacc ataaactgaa
    19141 taactttgtc cagcgtggac tcgacaacca tttacgcctc gccgttaccg gcttaagccg
    19201 tagtggcaaa acggcattta ttaccagctt ggttgatcag cttttgcata tcaacaaagg
    19261 cggtaattcg catttaaacc tgtttgcgcc tgcacgccac gggcaaattc tatcggtaaa
    19321 acgcgttgag caaggggatt taactattcc ccgctttgaa tatgataaaa accgccaaag
    19381 ccttgaaaat caaccacctc aatggcctga atccaccact ggcattagcg aaattcgcct
    19441 tgcgattcgc taccagcggc aacattctct gctacgtcat attaaagaaa ccggcacgct
    19501 ttatttggat attttcgatt acccgggcga atggctgtta gatttacccc tgctctccct
    19561 ttcctataaa gaatggtcgc aaacgcaaca aaccatacat aaagatgagc gtgcagagct
    19621 tgccacacct tggttacaag cggttaaaaa actcgatctt tttgcaaaaa ccgatgaaaa
    19681 tttactggca gaattcagcc aacaatacac cgattatttg ctcgcctgca aagctcaagg
    19741 aatgcaatat attcagccgg ggcgatttgt gttgccaact gaaaatgcca aaggtgcacc
    19801 ggtattccaa ttttttccgt tattagattt aagcgaagct gagtgggaaa agttggaaca
    19861 aagcgacaga aacagtattt tccataccct caaaaaacgc tatgagcatt accagaaaaa
    19921 aatcgtcaaa ccgttttatg aagattactt ctctcaattt gaccgccaag tgattttggc
    19981 agactgcctc acaccgttaa attatggcta taacgaattt atggaaatga aattagggtt
    20041 acagcaactg tttaaacatt tccattatgg caatcgttcg ctatttcacc gtctgttttc
    20101 gtccaacatt gataaattgc tatttatcgc aacaaaggca gatcacatta ctagcgacca
    20161 agtgccgaat ttggaaagcc taatgcgaca attagtgcaa gaagggggaa gacacgcaga
    20221 atttgatggt atcgaaaccg gctatttagc catttcctcg attcaagcaa ccgatgcggt
    20281 gaaagtgact cagcaagata aaatcattaa agcgataaga gggatccgtt ccagcgatag
    20341 aaaacaagtg acactctacc ctggcagcgt gccaaatcgc atacccgata aaaattactg
    20401 gcaatttaac cgttttgaat tcgaccaatt cgagcccaag caacttgatt atagccaagc
    20461 gattccacac ttcagaatgg attcggtgct acaattttta ttggcggata tgtttgagta
    20521 aaatcttgtt gcatatttcc attaaaggta taaaatttta tacattatga atactgtaat
    20581 ttcccaacga aaaccaattc actgggttgg atcaacctta gatgatattc gtgaatttcc
    20641 ggataacatt aaaagagaac tcggttttga tcttgattta gttcagcaag gcttattacc
    20701 gagggatttt aaagcgatgg caaatttggg ttcaggcgta atggaaattc gagtaaaaga
    20761 tccctcaggg gcttttcgct tagtgtattt agctaaattt aaagatgcaa tttactgttt
    20821 acacgctttc caaaagaaaa cgcaaaaaac agcccctaaa gacattgctg tcattaaagc
    20881 acgctatgct gccgtaaagg agttagaaaa tgaataatat tgatactaaa attcgccata
    20941 ctacagaagc tcagggtaat gtttttgaag atcttggttt ccctattgat gaagctcaaa
    21001 aattgaaatc tgccagtcag caactgattg aaactaaact gatgctaatg aatgaaatga
    21061 gcaactggat tgataaaaat aatctcaaac aatcagaagc ggcttctatt ctaggtgtta
    21121 gccgccctcg aatttctgat atggtcaatg gcaagttaga aaagtttacc ctagatacct
    21181 tagtcacttt tgttgcaaaa accggcaaaa caatccaatt gcaattatcc tgatatttgc
    21241 aaaattttac tcaaattcaa ccgcttgttg cttttatcat tcactaacaa ggttatttca
    21301 agtaaaattg tgactaattt tttattaata aacacaaatt atggcaaaaa aacctaaagt
    21361 tgcatcaaat acgattgcgt taaataaacg tgccagacac gaatatttca ttgaagatga
    21421 aattgaagcc ggcttagagc tacagggctg ggaagtcaaa gcattgcgtg ccggaaaagg
    21481 caatatcagc gatagctatg tcatctttcg ccacggcgaa gccttcttat tcggggcaat
    21541 gattacgcct ttgaatatgg catcaaccca tattgtcagc gacccgaccc gcacccgcaa
    21601 acttctactg aataaacgtg agttagactc actgttcggt aaagttaatc gtgaaggtta
    21661 taccattgtt gccacctcaa tgtattggaa aaatgcgtgg gtgaaagtaa aaatcggctt
    21721 agccaaaggg aaaaaactgc acgataaacg tgaagatatt aaagaccgtg aatggcaagt
    21781 ggctaaacag cgtattatga aaaacgccaa tcgtggctaa atgaataatt accctctccc
    21841 cttgtgggag agggacagaa aaattgcgtt cagcaatttt tcagggaaag ggggatttgt
    21901 aaaagttcaa ggaaaaacaa cgcttgccct ctctctgaaa aatatttctg aacgaaatat
    21961 ttttcggtct ctctcccata aagggagaga gtgaaacaca aatttaaact aaggaacata
    22021 caatgatcga ccaaaaccta cttcgtacca atttagaaga cgttgctaca gctcttaaaa
    22081 tcaaacgtaa ttttatttta gatgtagagc gagtaaaaac ccttgaagaa cagcgtaaaa
    22141 cgctacaagt taaaaccgaa accttacaag cagaacgcaa cactcgctcc aaaaacatcg
    22201 gtgcggcaaa agctcgtggc gaagatattt cagctttatt agccgaagta gattcaatgg
    22261 gcaacgaatt agacacggca aaagtagaat tggataaagt acaggcagaa atccgtgagt
    22321 tgttgctttc tgtgccaaac ttaccggcag atgaagtacc actcggtaaa gacgatagcg
    22381 aaaacttaga agtattacgt tggggcgaac cacgtcaatt cgactttgaa gttcaagatc
    22441 acgttaccct tggcgaaaac ttaaacggtt tagattttgc tgccggtgta aaattaaccg
    22501 ccagccgttt tgtggtaatg aaaggtaaac ttgcccgctt acaccgtgca ttatctcaat
    22561 ttatgttgga tttacacact gaacaacacg gctatgtgga aaccaatgtg ccatttttag
    22621 tcaatcacga cactctatac ggcacaggtc agctaccaaa atttggcgaa gatttattcc
    22681 atactcaacc attaacaggg caagatccaa acgaggtgca acgcccattc ggtttgattc
    22741 caaccgctga agtgccagta acgaacttag tgcgagatga aatcattgat gaagacagct
    22801 taccactgcg ttataccgcc cacacccctt gtttccgtgc agaagccggt tcttatgggc
    22861 gtgatactcg tggtttaatt cgtatgcacc aatttgaaaa agtggaaatg gtgcaaattg
    22921 tcgcccctga aaaatcaatg gaagccttag aagaattaac cggtcacgca gaaaaagtct
    22981 tacagctttt aggcttgcca taccgcaaag tgctactctg cactggtgat atgggctttg
    23041 gtgcagcaaa aacctacgac ttagaagttt ggctaccggc acaaaacact taccgtgaga
    23101 tttcttcttg ctcaaatatg tgggatttcc aagcccgccg aatgtctgca cgttgtaaag
    23161 ctaaaggcga caagaaaact cgcttggttc acaccttaaa cggctcaggt ttagcggttg
    23221 gtcgcacact ggtcgctgtg ttagaaaact accaaaatgc cgatggctct attactgtgc
    23281 ctgaggtgtt aagaccttat atgggtggtt tggaagtaat taccgcttaa gattaataaa
    23341 tgaaaccaat ctcagaaatg gggttggttt ttttaataaa tcttatccct aatctttttc
    23401 aggataattc ataaaaattt cagtaactac tcggcggttt tgcactgtat ctaaacgaat
    23461 aggatttttc attctcccta aacgaaatgc aataatggtg acttttttcg gttcatctat
    23521 tttatctaat tgatagaata ccattatttc ccgttgataa accgctcgct ttctgaaacg
    23581 taaatccttg cgaaatgcga cacctgcttt tggataaaac tgtaaaccat tgatgatatt
    23641 cgtttgagta tgctccgaaa ataatccata gaaaataaac tctaatagat tatttttaaa
    23701 atcaacacta accatctaac tgctcccaag cagttaaatc tcctcgtgat aaacgttcag
    23761 caatatcagc ataaaattgt ttcggtactt ccctttttac ttggtattga gctgattgtt
    23821 cttctaataa atgttcttct atcatcttga tttcttctgt tgtccactct tcatcttctt
    23881 tatgtacaac aaaatcggta tctacaatca caatagacat acctagctcc ttcaagtact
    23941 ggttatatat tctgtatttt gcctgttcta aattcagctg tctagctttt ctgttactca
    24001 caatttgcaa atttttcatc aaattccacc gcttgccatt caaaaaaagg cgtttttgcg
    24061 gtatcctatc ggcaattttt tcagttaaat ttaaaggtta aattttatgc aacagcagta
    24121 taacccaagt gagattgaac ctaaggttca gcgttattgg gcagaaaata aagtttttaa
    24181 agcagttaaa gacgttacca aagagaaata ctattgcctt tcaatgttgc cttatccgtc
    24241 aggtaagcta catatggggc acgttcgtaa ctacaccatt ggtgatgtag tgtctcgtta
    24301 tcagcgtatg atcggtaaaa atgtattaca gccgatgggt tgggacgctt tcggtttacc
    24361 ggcggaaggt gctgcggtga aaaataatac cgcaccggca aaatggactt acgaaaacat
    24421 cgaatatatg aaaggtcagc ttaaaatgtt gggcttctct tatgactggg atcgtgaagt
    24481 caccacttgc cgtccggaat actacaaatg ggagcaatgg ttcttcacag aactttataa
    24541 aaaaggctta gtgtataaaa aaacctcaac cgtaaactgg tgtccgaatg atgaaactgt
    24601 gcttgccaac gagcaagttc acgaaggttg ttgctggcgt tgtgacaccc cagtggaaca
    24661 gcgtgaaatt ccacaatggt ttatcaaaat cactgactat gctgaagaat tactcaatca
    24721 gttagataat ctcccacagt ggccggatca agtaaaaacc atgcaacgca actggatcgg
    24781 tcgttccgaa ggggtagaaa tcacctttaa aattgccggt tcaaacgtag aattaccggt
    24841 ttacactacc cgtccggata ccttcttcgg cgtaagctat gtggcgattg ctgcggctca
    24901 cccattagca gaattagctg cacaaaataa cccggcattg gctgaattta tccgtgaagc
    24961 gaaaaacacc aaagtggcag aagcagaatt agccaccatg gagaaaaaag gaatggcaac
    25021 cggcttgttc gccattcacc cgttaacagg aaaagaagta ccggtttggg ttgctaactt
    25081 tgtgttaatg cactacggta caggtgcggt aatggcggtt ccggctcacg atgagcgtga
    25141 ctttgaattt gcacaaaaat acgatttgca aattaatcag gtaattcaac cgcttgatgg
    25201 ctcagagtgg gatttcagca aagccgctta caccgaacac ggtaaattaa tcaactctgc
    25261 cgagtttgac ggcttggaat ttgatgtggc tttcactgcg attgccgata aattagaggc
    25321 aatgggtgta ggtaaacgcc aagtgaattt ccgtctgcgt gactggggtg tttctcgtca
    25381 acgttattgg ggtgcaccaa tcccaatgat gaccacagaa gagggcgaag tggttaccgt
    25441 tccattacaa gatttaccgg tgattctccc tgaagatgtg gtaatgaacg gcgtgcaaag
    25501 cccaatcaaa gccgacccag agtgggcgaa aaccacttac aacggcaaac cggcattgcg
    25561 tgaaaccgat accttcgata cctttatgga gtcctcttgg tattatgcac gttatacttg
    25621 cccacaatat cacgaaggaa tgttagattc agacgaggca aactactggc taccggtcga
    25681 tcaatatatc ggcggtattg aacacgccac aatgcacttg ctctacttcc gtttcttcca
    25741 taaattgttg cgtgatgcgg gtattttaaa ttcggacgaa ccggcgacta aactgttatg
    25801 ccaaggtatg gtgcttgccg atgcgttcta ctacaccagc ccaactaacg agcgtatttg
    25861 ggtcagcccg acacaagtca ccttagaacg tgatgagaaa ggtcgtatca tcaaagcaac
    25921 cgatccacaa gggcgtgagc tggttcacag cggtatgact aaaatgtcga aatcaaagaa
    25981 caacggtatc gatccgcaag aaatggtgga aaaatatggt gctgacacag ttcgcttgtt
    26041 tatgatgttt gcctcccctg cggaaatgac attagaatgg caagaatccg gcgttgaagg
    26101 ggcaaaacgt ttcttaggtc gtgtttggaa tttagtttat agctatgccc aaaatccggc
    26161 aacgactgca ttagatgtga atgccttaga caaggatcaa aaagaacttc gccgtgatgt
    26221 acacaaaacg attgctaagg tgagtgatga tattggtcgt cgtcaaacct tcaatacagc
    26281 gattgctgcg gtgatggaat tgatgaacaa gctcaccaaa gccccacttg aaaccgaaca
    26341 agataaagcc gtaatggcag aagctttaag tgcggtggtg cgtatgcttt acccaatcac
    26401 gccacatatc tgctttgagt tatggcaagt gttaggtaat gaacaaacca tcgattttgc
    26461 cccttgggta caagccgatg aatccgcaat ggtggaagat gaaaaattag tggtagtaca
    26521 agtcaacgga aaagtgcgtg gcaaaatcac tgtttcagct actgctacgg aagatgaagt
    26581 taaagctatc gctaaagccg atccaaacgt tgctaaattc ttagatggtc agcagattgt
    26641 aaaagaaatc tacgttccat ttaaaatgtt gagctttgcg gttaaggcgt aacaaacaca
    26701 ctctccctta tatgggagag agaccgataa atatttcgtg cagaaatatt tatcagagag
    26761 agggtacaag cggttatttt cttggaaaat tttgcaaaaa atagccccct ctccctgctt
    26821 tttcttctaa acgaagaaaa acctgtccct ctcccacaag gggagagggg aataaacaag
    26881 gaaatctcat tatgattaaa aaactctctc tcatcgctgt ttttgctctt accgcctgcg
    26941 gttggcactt taaaaacaat gaggtattac ctgaatcgtt cagaacatta acctttgaaa
    27001 gtgcagatca acacagcgaa atgtctcgaa ttttacgcaa tcagttacaa ctaagcgatg
    27061 taaaattagt gcctagcacg gctaatgtgg caaaattacg tttggtttca acctcaacag
    27121 acagcaaagt ggtttctgtg tttaaacaag ctcgtgaagc agaaaaaatc ttaacgctaa
    27181 acgttgatgc gattgccgat attccaaata aagggcaata ccctttagaa gtttcggttc
    27241 atcgtacttt ctttgatgac tctcgtgcag ctttggcaaa gtctgctgaa aaagagatga
    27301 ttatgaaaga tatgtatgaa caagcctctc gccaattaat tatcaaaatg gcgggcttaa
    27361 aaaaagaact ctcaaaataa tcattgtagg ggcgaaatat gtttcgccca ttgtttatct
    27421 gtatgcaaaa aatctttccg gaaacacttc aattttcatt aaacaaagga ctacagccct
    27481 tttatctatt aacagggaat gatctgctat tggtgaatga aaccaaagat gccattattc
    27541 acaccgcccg tctcaacggt tttgatgaaa aaaaagaagt tgaaatcaaa aacgatacta
    27601 actgggaaga acttttcgaa gcagctcaat caatgggatt gttttttgca cgccaaatta
    27661 tggtgctgaa ttttccggat tcccccacag cgacacagtt taaaaaactg gctgagcttt
    27721 gtgcctttat tcatagtgat ttgctcttta tcctgcactt acctaaattt tccaaaacaa
    27781 tggaaaaaca ggcttggttc acgcaaattg agctaaatct cgtgcaaatt aactgccaaa
    27841 caccggaact tagcaaactt ccacaatggg tcagtcatcg agcaaaagcg atgaatttgc
    27901 aaattgaaaa tgaagcaaac caactccttt gttatagcta cgaaggcaat ctactggcgc
    27961 ttaaacaggc attacaactg ctacaactca atgcaactga tggaaaaatc acgctgacaa
    28021 aagcccaaga agtggttgaa cactccgccc aatttactcc atttcaatgg attgatgcat
    28081 tatttgaagg aaaaatccaa agagctcaac gcattttgta ccatctgcaa aatgaagaag
    28141 tacaagcggt cgttttatta agaattgtgc aaaaagagtt gatgatttta ctcgaaatca
    28201 ctcgcaaccc aaccgcagtc gtttccagcg aacaatcact ttttatcggt aatttacgaa
    28261 cagaatttga ccgcttgaaa atttggcaaa accgcagacc tttttacaac cagatcactc
    28321 aacgactgac ttaccaaaaa ctttacgccc taatccaaca actggctgaa ttagagcgta
    28381 aggtgaaaca agaatttagc gatgatattt ggcaagaatt agagcgtttt tccgctcaat
    28441 ttacctaact aatacttttc taaatcttcg ctaaacaact ccgcttcttc actggcgata
    28501 gagtgctttt ctgccgccca atcgcctaaa tcaataattt gacaacgctc gctacaaaac
    28561 gggcggtatt tactttcttt actccacacc acttcttttt cacaagtcgg gcagtttaca
    28621 atagtttctg gcattctttt tccttttttg ctaatgctaa gtatttataa tgtaaggctt
    28681 gaacctgctc tttaattaaa agcaagccct gttcaaatgg caagttattt tcaatcacat
    28741 catcagcata acttaatctt tcttcacggc tgacttgggc tgccataata tttttaatgg
    28801 tttcaatttt actgttatca cgtttggttg ctctttccag ttggatttca ggagaaacat
    28861 caacaaccaa tatacgatca caatattcgg tgagcttatt ttcaattaaa agcggcacga
    28921 cccaaatcac ataacaagcg gtcgattctt gcaatttttt taccatttct tctcgaatgg
    28981 cggggtgaag taaattattg agccacgttt tttcctcttc attgttaaac acaatttggc
    29041 gaagtttagt gcgttctaat gctttttcag cggttaagat ctcttcacca aaatgctcta
    29101 caatcttggc taaaagcggt gtacccggtt caacaacttg gcgagccacc acatcagcat
    29161 caattacctc taccccaaga gctgaaaata aatcagacac tgtggttttg ccactgccta
    29221 ttccaccggt taagcctaca acgtaagtca taaaaattga ttcctcaaaa taaaaacaaa
    29281 ccaattctag taattaatcc ctctgctttc aagcattagc accagcaaca tcgctgtgct
    29341 aataaacgga ataaacgcca attttgctaa tttctgcttt ttcattagcc aataagccaa
    29401 gtaataacca atccctagca ggctggcggt taatatcaat aagagcattt tttcgagccg
    29461 aaagagtggg gataacgcaa ccaataacaa catatcgccc gagccaaatg cctccttttt
    29521 aaacataagc tgtgaaacca agccaatcaa caggcaaaaa gcaagggtaa aggtaaaagt
    29581 ttggtgttgg aaatcggcta aactctccca taacaccaac aaaaatataa gcacgaggta
    29641 gcgaatatcc gttaggtaat agcacatatc cagcagtgat aaaaaacaga gcagaataaa
    29701 aatcgtgccg ataatagggc tttccgagca gagcataatc aatggaaaaa ggagaaaaaa
    29761 tagatttgcc gatcggggtt gtttcggttg tagcaccgat tgagccaaaa actgttgctc
    29821 ggttaagcca atattcacta atgaagttgt ttcttggtaa atttggcgat tgactcgaat
    29881 ggcaaatcga gggatctccg ctttgagcca aaccgcaatg aagagggaaa acagtattaa
    29941 catcgctact ccaccaaagc ccccatatca aaaatcggta aatacagccc aacaataata
    30001 gtgccgacaa tcacgcccat tatcaacatt agcatcggtt ctaatagctg agacaaaata
    30061 tcaatttggt aatctaattt ttgttggtac atctcactaa tatgctcaca cattttcgcc
    30121 acattgccac ttttctcccc aacctcaagc atttgtgcca tctcagcacc aaacacattc
    30181 gggttgatcc cttgtgaaaa tttgtagcct tgttgtaaca aattaagcat agtgccaatt
    30241 tcttgctgta aaatcgggtc atcatttttc tcggataaaa acgacctcaa ggcaacatca
    30301 agcggaatat gagcattcag cattaaggca atattttggc tgaaaaagat aatgcgagat
    30361 tgctcaataa ttcggctaaa cagtggcatt ttagcccata gagccatttt ccactttgcg
    30421 actaacctgc tcttttttgc aaaaaacagg taaaaaatga ccgctagtag taacgtgcca
    30481 agtaatactg tgccttgctc aatcaagcat tcggaaaggg taaataaaat atcggtaatg
    30541 aggggtaaag ttttgtcttt tgagccataa agttgggcaa actgcggtac aatcaaaacc
    30601 aatagcccga tagataaagc gagcgaaatc agcaacacaa tcaccgggta aaacaggatt
    30661 ttcttcactt ttttcgccaa tttttccaat ttcgctcggc tttcagctaa attagtcagc
    30721 ataatgccca gttttccgct ttgttcgccc attttgatta gctgaatttc ctgattggtc
    30781 acaaattcac ccaatttctc caacgattgt gaaaaagcat agccactttc aatatgctga
    30841 atcagctcat taagccatag ataaaggcgg atatttcggc agttttgcaa caacatagat
    30901 aatgcctgtt ttaacggcat ggcagaatta accaacaatg caagctgtga tagcaagcta
    30961 ttcacctctt cttttttcgg gtttgctgaa agcacaaaat ttcggctaat ccgaatatgc
    31021 tgataacctt gtgccagcaa aaggttttcc aacatgtctc gattcgttgc caaccgcttg
    31081 ccccgctgtt tttgcccaaa tcggctctgg gcttgccaat gaaattcata gattttcgtc
    31141 attgcctaag caatttctct ttttgtggtt caaaatgaat atctgaggcg ttgtgttgca
    31201 aacagaactt aaataaattc tctaaaattt ggataatcgg atcgtttcta tccacttgtt
    31261 ccgaatgagc caatgaatac gccgtattct cctcataaat ctcactctgc cgactactct
    31321 gctcaggcga aagggcattg agtaaaaact tcaattcatc ggaggaaatc aaaacaggct
    31381 caatctgttt gtggtagata aacgcaaaaa tctcacaggc attgagattt tgcatatcat
    31441 caatccctag ccaaagctgt tcttctgttt cttgcagcgg aatggctaaa tatcgcaata
    31501 aaatctgctt ttcattgcaa tttttctgcc actgctctgc tgaaatctcg atgaaccttt
    31561 ggctgtttaa atccgttacc gaatactcca ttttgatttc ctcacctcaa attagccgtt
    31621 tggtgtaaca gttgtgctac agaaattagc tggaaatagg ctcgtatcat tgccactaca
    31681 acttgccgtc caactaatcg tattatttga gaacgacggt gttaaggtat agccataacc
    31741 attcagagag cctttgccgg ttaccgtaat cacgcctttt gttaccgcaa ttgagtttag
    31801 gtatttagtg gtattactac tggttttatt cgcctgaatc ccattcgtgc caccggagca
    31861 gttatccaac ccgccactgt tgtaaataca gacttcaacg tctgatttat acgaagcaga
    31921 tgcttgtaat aactccgata acgccgcctt ttgggtgtat gagttatagg aagggatcgc
    31981 aatagtggca agaatggcaa taatcgcaat cacgatcatc aactcaatga gggtaaatgc
    32041 ccgatgcagt tggcgaatgc caagcggtct aaaatgtaca attttttgca tataaattcc
    32101 ttataaattg caccaacaaa ttgtggcgaa tcaagtgtgc aaattttttg cctgctttgc
    32161 gaccggcaaa ttttaatttt tcgacgcaga tcgaaaaaat aaaattttta tctgtttatc
    32221 catacagttt tttgttagaa tttgccaaat ttactcaatt aggaatcaag aatggcaaaa
    32281 gcacctaaaa ccgcttatgt ttgtaacgat tgcggggctg aatattcccg ttggatgggg
    32341 caatgtaagg aatgtaaggc ttggaatacc atcagtgaag tgcggttgat ttccagcaaa
    32401 gaagcggcga aatctgaccg ctttagcggc tatgccggtg aaacgtcagg caaagtacaa
    32461 acgctgtctg acatcagctt gcaggaagtg cctcgcttta gcagtggctt taacgagctg
    32521 gatcgtgtac tcggtggcgg cgtagtgccg gggtcagcaa ttctgatcgg cggtcacccg
    32581 ggggcgggga aaagtacgtt attactgcaa gtaatgtgcg gtttagccca acatctgcca
    32641 acgctgtatg tcaccggtga agagtcgtta caacaagttg caatgcgggc aaatcactta
    32701 ggcttaccaa cggcaaatct gaaaatgttg tctgaaacct ctgtcgagca tatttgcaat
    32761 attgcagatc aagaaaagcc caaaattatg gtgattgact cgattcaggt gatgcacttg
    32821 tcggatattc agtcctcacc cggcagtgtt tcacaagtgc gtgaatgtgc atctttcctt
    32881 acccgctacg ccaaaacccg ccaagtggcg attattatgg ttgggcacgt cactaaagat
    32941 ggcacgcttg ccggcccgaa agtgttggaa cacgccatcg atgcttcctt gctgttagaa
    33001 ggcgaatccg attcccgttt ccgtactttg cgtagccaga aaaatcgttt tggtgccgtc
    33061 aatgaattgg gcgtatttgc gatgaccgaa caagggctaa aagaggttaa aaacccatct
    33121 gcgatttttt taagccgtag cgaagagcaa acgccgggca gttcggtgat ggtgttatgg
    33181 gaaggaacac gcccattgtt ggttgaaatt caagccttag tcgatcactc aatgctggca
    33241 aaccctcgcc gtgtggcggt tggcttagat cataaccgtt tatcgctatt acttgcggtg
    33301 ctacaccgac acggtggctt gcagatgtcg gatcaagatg tgtttgtaaa tgtggtcggt
    33361 ggggtgaaag tcacggaaac cagtgccgat ttagccttaa ttttggcgtt aatttccagc
    33421 ttccgtaacc gtccattgcc gcaagatttg gtgatttttg gggaagtcgg cttagcaggg
    33481 gaaatccgcc cggttaccag tgggcaagag cggatcagcg aagccgcaaa acacggcttt
    33541 aaacgagcga taattcctca cggcaatgtc ccgaaaaagg caattaaaga tatgcaggtg
    33601 ttcaccgtga aaaaactcag tgatgcgtta gatattctgt ataatctcta aaatcaagaa
    33661 ccgcttgtaa gtctgtctta caagcggtct gttttaggta aaaatttacg aatgtgcggc
    33721 agttggtcta ttgcctttgg aaatatagta taaattaata cacaccacca acgcatttaa
    33781 gccggtgacc ggaatcgagc caatcagcaa accataaaca acgaaacagg cacaacccag
    33841 cgaattcacc acccgcagtt taatcacatc ttttaataaa aaagaacagg caactaaaat
    33901 catcgccaca tagccgagta tttcaacata gtccatttag aacctcctca aataaaataa
    33961 actgccgata ttttaaaggc tgttttttta tttgtatcta gctctaaaaa tagcaaaggt
    34021 ttgcgttcac atttttactt ttaaccgctt agttattaaa cagattttcg ttctaaacac
    34081 ataaaacggc aacggaaagg gttattttca tcaatttctg accacttttc ttctactaac
    34141 gcccaatttt gttcatcaaa ttcaaagaac gtatcccctt caatgtctgc ttgaatctca
    34201 gttaaataaa gtctttctgc tagtggtaag gcttgcttaa acagttcccc cccaccaata
    34261 atcattattt cagctgtatt tgcaaaattt ttcgctaatt caaccgcttg ttcgagcgat
    34321 tgtaccgcat aagccccttc caccgtaaaa cctgaacggg aaagaataat attagggcgt
    34381 tttggcaata acctgccgat agattcatag gttttccgcc ccataatcac cggtttgccg
    34441 agcgtgtttt ggcgaaacca ggctaaatct accggcaaat gccacggcat ttggttatct
    34501 ttgccaatca ccttgttgat tgttcgggca acaataatgc tgattttcat tttttctcct
    34561 gttatgcaaa ttggtttgat tttatatcaa attaagcgtg attttacacc ctaaaagtga
    34621 tacttttggc aacattattt ttagttacaa gtgaacagat tatgtcaaaa actatcgtga
    34681 taaaatttgg caccagcact ctcacacacg gcactaaaag tttaagccgt ccttatatgt
    34741 tagagctggt aaagctcaat tacaccaaca gcatcgtgtg attgtggtga catccggagc
    34801 tgccgctgcc ggtcgggact atttaggaca ccctgaactc cctaaaacct tagcctctaa
    34861 gcaaatgtta gcagcggtcg ggcaaagtca gctcattcga gtgtgggaaa atttattcga
    34921 tatttataat atccatatcg ggcagatgct cttaacccga gccgatctgg acgatcgtga
    34981 acgtttcctc aatgcccgtg atacccttga tgccctgctt gcacaaaaaa tcattccggt
    35041 gattaatgaa aacgatgccg ttgctaccga agaatttaaa gtaggcgata acgataattt
    35101 atcggcttta gttgcgattt tggctcaagc agatcagctc tatttattaa ccgaccaaga
    35161 aggcttattc agtgccgatc ctcgctcaaa ccctgatgct aaattgctct cggtggtgga
    35221 aaaaatcacg cctgaaatcc gccaaattgc cggtggtagt tcaaccggtt taggcacggg
    35281 cggaatgagt accaaaatta ctgctgctga tattgctacc cgctccggtg tggaaaccat
    35341 catcgcctcc ggttctcgcc caaatgtgtt agttgattta gcaaacgggc aaaacatcgg
    35401 cagtaaattt ttagtccaat ctgaccgctt ggaaggcaga aaacgctggt tatttgctgc
    35461 tccgcaagtg ggtgaaattg tggtagattt cggggcggaa caagccttgc ttcaacataa
    35521 atcattactg ccggtgggag tttctgccat caaaggcgaa tttactcgag gtgaagtaat
    35581 caaaattttc agccaagacg gcaaagcctt agcactcggc attgcccgtt atagcagtga
    35641 ggcattgctg cgaattaaag gtaagaaatc aaaagaaatt gaatcgctcc ttggttatga
    35701 atttggttct gttgcagtgc atagtgacga aatggtggtg tattaatgga attgggaatt
    35761 gatgttttag caatcttgtt tgccgttgcg acccttgccg gctttatcga tgccatcgct
    35821 ggcggcggtg gcttactcac tattcctgca ttaatggcag cagggcttcc tccggcaatg
    35881 gcacttggca cgaataaatt acaggcgtgt ggcggatcac tctcttcctc actctatttt
    35941 attcgcaaaa aagctgttaa tttaaaacaa atttggttgc taattctatg cactttttta
    36001 ggctctgccg ccggtactat ttttgtgcaa atgatcaatg tcgatttctt aaaaacattg
    36061 ttgcctttct taattctgat tatcggcatc tattttttgg tcagccctaa aattggcgat
    36121 gaagatcgta aacaacgcat tagctttacc ctatttgcct ttaccgccgc tgtcgctctc
    36181 ggtttttatg atggaatgtt cggaccggca accggctctt ttatgactct ggcatttatt
    36241 ttgctgctcg gcttcaacct ctctaaagcc gtagcacacg ccaaagtgat taattttacc
    36301 tccaactttg cctcattaat tttctttacg cttggtggtg cggtaatgtg gaaaatcggc
    36361 ttaattatga tggtcgggca atttattggc ggcacactag gtgcgaaaat ggtgatgaca
    36421 aaaggcaaaa aattaatccg tccgatgcta gtgactatgt cttttatgat ggtcggcaaa
    36481 atgctctacg agcaaggaat ttttaattaa caatgaataa agcaaaacgt attgagattt
    36541 taacccgttt acgcaatgaa aatccaaaac cgacaactga actcaattac agtaatccgt
    36601 ttgaattatt aattgccgtg attctatccg cccaagcgac cgataaaggc gtaaataaag
    36661 ccaccgataa gctcttccct gtcgccaaca cgccacaagc gattttagat ttaggcgtag
    36721 atgggctaaa agagtacatc aaaactatcg gcttatttaa cagcaaggca gaaaatatta
    36781 tcaagacctg ccgagatctg atcgaaaaac ataacggcga agtgcctgaa gacagagatg
    36841 ccctcgaagc ccttgccggt gttggcagaa aaaccgccaa tgtggtgatg aataccgcat
    36901 tcggacaccc tacgattgca gtcgataccc atattttcag ggtgtcgaac cgcacgaact
    36961 tcgctcccgg caaaaatgta gttcaggtag aagaaaaact gctcaaagtt gtgccggacg
    37021 aatttaaagt cgatgttcac cattggttga ttttacacgg acgctacact tgcattgccc
    37081 gtaagcctcg ttgtggttct tgtattattg aagatttatg tgagtttaaa gagaagacgg
    37141 aataacatta tctggtggta tttaatgaat aactcccctc tttagaaaag taggggctgg
    37201 gggagatttg atactatttc aacatttcgt atgagcgtat agaaattctg ccaaatcttc
    37261 cctatcccct ctttgctaaa gaggggaact agatcgagcg taaaatataa caagcggtca
    37321 tttttttgag attttttgca aatggatatt tatttagtcg gtggagcagt gcgggatcaa
    37381 cttcttgggc tgccagtgaa agatagagat tttttagtgg ttggcgcaac tgccgaacaa
    37441 cttttagccc aaggatttca acaagttggg gcagattttc cggtgttttt acaccctgaa
    37501 accaaagaag aatatgccct tgctcgccaa gaacgcaaga acggcaatgg ttacaacggc
    37561 tttatttgcg atttctcccc aaacgtcacg cttgagcaag atttgatccg ccgagattta
    37621 accattaacg ccatcgccca agatgaaaac agcacacttt ttgatcctta tggcggtgtg
    37681 caagatttag aaaatcggct actccgccac gtttctcctg cattcagcga agatccgcta
    37741 cgggtgctac gggttgcccg ctttgccgcc cgttttcatt catttggttt taccattgct
    37801 cctgaaaccc tgaaattaat gcgagagatg gtacaaagcg gtgaattgaa acatttaacc
    37861 gctgaacgtg tgtggttgga aacacagaaa gcctttgaaa ccgataatcc gcacgtttat
    37921 tttgacattt tacgcttaat tggggcatta aaagtgctat tccctgaact ggatcgtctg
    37981 tttggcgtac cacaaccacc tcaacaccac cctgaaattg actccggcaa acacactcta
    38041 atggtgctac aacaggcaaa aagacttgca aaaaaagcgg aaaatccgac cgctttactc
    38101 tttgcagccc tttgccacga cttaggcaaa ggcttaacgc ctgctgatat tttgcctcat
    38161 cactacggac acgaagtcaa aggcattcaa ccaacccgag agttagccaa tcggttaaaa
    38221 atcccaactg agattaaaga ttttgccctt ttagtgaccg aataccacac gcattgccat
    38281 aaaatggcgg aacttcgccc tgaaacggta ataaaactgt ttaacagcct agatgtgtgg
    38341 cgtaaaccaa accgcttttt tgatttcctg ttcgcctgtg aagcggatgc tagagggcga
    38401 ttaggttttg aacagcggaa ttacccccaa cctgaattgg cgaaaaaata ttaccaagcc
    38461 gcaatcgaaa ttgatgtgca gcaagttatt caagacggtt ttgaaaaaca agcaattcgg
    38521 gaagagctga ataaacgcag agtgtatgcg gtaagaagca tcaagctaga tagcgaccta
    38581 ataaaccatt aggttatccg ccactttctc aaaatcaatt cccaaagttc aagttggtta
    38641 aatgtatgga ttctttaaaa ggtggcgtta ttttagtgaa tttataatgt tcctatgctc
    38701 ttgattgttg tagccacaat ttcagggtaa agatcagcaa taacccaatg ccgaaattgg
    38761 caatagtgaa gccggctaac ccaataaaag ataaaggttc aaattgataa aattcaatcg
    38821 cacctcttgc cattgcagct aaaccaaatg aaaatgccca gaaacccatc gagaagccat
    38881 tttcagctat ccatttgagg tttcgtaaaa taaaacagaa ttgtaagaat ccatatcccc
    38941 ataacatttt tgccaacaag tctaattctc cacccataga aaaataggca gaagcgccca
    39001 caaatgccgg agcaagaaca attcctaaag atggtctagc aatcggaaaa agcggactaa
    39061 gccgaattct ttgtaacata gtgggttcat atactaacca ggaaattatg ccaatgccta
    39121 aaaataaaga tgcaatttca ctgtagccaa ttaagcttaa tgaacttgcc atagtgaagc
    39181 tccctgcaat tggcggtagg taatgtaccg ggtgtagcgt ttccggcaaa aattgtgtgc
    39241 ctttccataa tccaccaata ataaaaacca agaaaattaa atgcacaatg acgcctaaaa
    39301 aaatcaatag ttcgcctaaa taaaaatccc atacatagag cacatcaccg cttatcatgg
    39361 ttgtcattgt cactaaggaa aataaacaac attgcactgg gtgcataaat tctgctttaa
    39421 tctgctcaaa acaaaaaaac acacgataca cataacagcc taataaggct acccaaagta
    39481 tgactgacgt tccagcaatg atattaccaa ttgttgtagc ataaggagtg ataggcgttg
    39541 catttaacca ggcaaggcct aacgccccca gccctaatgg cattgcaaaa aagcctgtag
    39601 ttattggaaa aggtttagat tcactcataa atttatcctc atattgaaat aatgcataac
    39661 tgtattcggc tttataacag tcatatgtaa aaaattttat ttttctaacc gcttgtaata
    39721 agaaaaacct aaagcacttg ctttaggttc ctactactat tcatactctt ctagccagat
    39781 agttaaaatc gcttcaagaa tatgttcatt tgatgcttcc ggatcatcat cgaaatcttc
    39841 catttcacaa atccatttat gcatatctgt aaaacgaacc gtggtcgggt ctaaatccgg
    39901 gttcatatca tacagatttt cagcgatcat tcgggtatct gtccatttca ttagtgcgct
    39961 gcctcatttg cgtgatttaa attatatttt ggaatttcaa caaccaaatc ttcttcacca
    40021 acaatacatt ggcaacttaa acggctgtct atttccaatc cccaagcttt atccagcata
    40081 tcttcttctt gatctgaagt atcatttaac gaatcaaaac cctcacgaat taccacgtgg
    40141 caagtggtac aagcacaaga acagtcgcaa gcgtgatgaa tttcaacgcc tgcttcgtgt
    40201 gctacttcta ataaattatc tccggcttta gcttcaacaa ccataccttc cgggcaaaat
    40261 tcttcgtgtg gaagaaaaac aatttttggc attttaaagt ttccttatta ctaatcccat
    40321 tcaatattat ccagatgttc gccgatgaag tttaaaatct cttcctcatt ttcaaccaga
    40381 taaacaataa tcttctttaa ttcccttgct ttctctttac ttttctcgga aagcggatag
    40441 gtaacataat aacatcctac gtccggatct tcttctaata actctaatag gcttggacga
    40501 taaatactaa gataagcccg taaaaatgca gcccagttat aaccgttcat ataggcaaca
    40561 tcatattcag tattaatccg ctcgcccaac tcaaaaatgc ggttatcctc taaatcgaat
    40621 tcaaccgcaa cggttctgaa ttgttctgaa atctcggttt ttacatagtt agacatactg
    40681 cctccttatg ctaaatcgtc cattttttta ccggctaatg ctttttgaat tgataaattc
    40741 atacgccgtg ctgcaaattc ttgcgtagct aaatccacct ctttgatacc ttgctgaatt
    40801 gcaaagcgat cagtaccttc ttttaattcg attaaacggg ctaattgtgc ttcaatcgct
    40861 tggaactcct ccacactcaa cactgcagag ccatcttctt tcaatgccgt taccacgctg
    40921 tcaatcactc ggtcggcttc tactcgctgt tcaactaact ggcgggcttc catatcttct
    40981 ttggcatttg ccattgagga tttaatcatc tgcgtaactt cttcatcggt taagccataa
    41041 gacggtttga tttgaattga agcctgcact ttggtcgatt tttccattgc agtcacgttt
    41101 aataaaccgt ctgcatcaac ttggtaagtt acccgaacct gtgcaacccc tgctgccatt
    41161 gccggaatgc cacgcaaagt gattttaccg agagagcgac aatcttcgac taattcacgc
    41221 tcaccttgta aaacgtggat tgtcatcgcc gtttgaccgt ctttaccggt agtaaactct
    41281 tgagctcgag ctactgggat agtagtgttg cgtggaataa ttttctccac caaaccaccc
    41341 atcgtttcaa taccaagcga aagtggcaca acatcaagca acaacatatc ggaatccggt
    41401 ttgtttccaa ctaaaatatc cgcctgcact gccgcaccaa tggcaacaac tttatccggg
    41461 tcgatagagg ttaatggcgt tttaccgaaa aactcgccga cttgttcacg cacaaacggt
    41521 acacgggtcg aaccgcctac cattaccact tcacgaacct cttctgcttc aactccggca
    41581 tctttcaagg ctcggcgaca agtcatcagc gagcgtttta caagcggttg aattagctca
    41641 ttaaattgcg aacggcttac tacgcctgtc caatttgcaa atttcacttc aaattcaacc
    41701 gcttgcgaaa gtgcgacttt ggtttgagtt gccaaagtta ataattcacg ctgctcgctt
    41761 gccgtttgag gtttgtaatt tgcctgttca gcaatccatt ccgctaatag gtggtcgaaa
    41821 tcatcgccac cgagtgcggt atcacctgcg gttgctaata cttcaaacac acctttgcgt
    41881 aaacgcaaaa tagataaatc gaaagtaccg ccacctaaat cgtaaacggc aatcacacct
    41941 tcttgtccgc tttctaaacc ataagcaatc gctgctgccg ttggttcatt gagcaaacgt
    42001 aatacattta aacccgcaag acgtgcggca tctttggtac tttggcgttg tgcgtcatca
    42061 aaataagccg gtacggtaat taccacgcct gaaagctcac cggctaaacg cttttcggca
    42121 aattgtgcca agtggcttaa aatatccgcc gaaacttcaa tcgggctttt attaccttgc
    42181 gctgtttgga ttaaaggtaa accgttttcg ctcgcgacaa attgataagg taaattttga
    42241 taacgttgct gaacatctgc cagagaacgc ccaatgagac gtttagctga aatgacggta
    42301 tttttcgggt caagtgcagc tttggcaaag gcttcaatcc caactgtttt ttctgtttcc
    42361 gcataatgca ctactgacgg cactaatgct ctttcttgtt catcaagcaa tacctgagtc
    42421 tgcccgctac gcacagttgc cactaatgaa ttggttgtac ctagatcaat tcctaccgct
    42481 aatttatgtt gatgtggtgc agcggtttga ccgggttcag aaatttgtaa taatgccatt
    42541 tatttatctc tttatctttc taacttagtt ctctattgta ttaaatggag tgaaatatat
    42601 tgtttaagag aagctggaga ttcccctctc cctgattttt cttctgaacg aagaaaaatc
    42661 tatccctctc ccacaagggg agagggttat tctttcaaaa attcaaaaca actaaaaaat
    42721 tagcattctc cttttgtagg agaaaatgtt gttctttcaa aattctaaaa taaccgcaaa
    42781 attaactctc gccccttgtg ggagagagac agataaatca ttcgttcaga atgatttatc
    42841 agagagaggg gaaattaccc tctcgtaatt tcataaaaaa ttgtatctaa aacagcctct
    42901 agatcgctat gaatttgatt attccaaaat cttaaaaccc tgaacccctg accgtttaaa
    42961 aaatgagttc gtagattatc atagttttgt tgatctaaat gttgtgaacc atctaattca
    43021 ataactaatt tcgctgaaaa gcaaacaaaa tcgacaatat agttacctat aacttgttgc
    43081 cgtttaaatt ttatcgctaa aaatcgtttt gctcttaatg cttgccaaag aaccttctct
    43141 gcttctgtca tattagtccg aagctgttta gctctttggc ataaaattgc tttcattttt
    43201 tattcctctt ttttaattta gtataaaagg aataaaaaat ataatttgag cgatttttgt
    43261 gatctaaatc gaaaatcaga aaatttcttt cgttctaaaa aaataagcta aacagcctat
    43321 atttcagcct ctcttacaaa cacttaagcc accgaatatc ccatattttc aggggcaaat
    43381 tcgccttggt agtggcgttc aatcggaacg gcgattttac ttgtaagatc cagcacttga
    43441 tgcccttgta atttctctac atcggtttgc cagttttccc aacgcaaatc ctgataaaaa
    43501 tggtttaaat ctccggctaa tgcccagcct agaaattctg aataacctaa ttgggtgttt
    43561 tcccattttt tagcttttgg atgataataa taaactttgc caagatctga gccgattccg
    43621 ccggcattaa tggcaaaata gccaccgatt gcatcatccg ctaccagcaa gtggcttggt
    43681 tgttcgccag attgtttaaa agttttgcca aagttccaat caaataagcc tcgaggcaat
    43741 ttttcgttgc ctgagcctaa aatgcgtaac caaccgtggt caataaatac gccaccggtt
    43801 tcatacacca ttgcccctag cggtgagcga gtagaaagtt gcataccaac caatgcactg
    43861 ccggcatctt cagggtaact cgctaaaatt tcgtaatgat ttttggcttg ggtaaaccag
    43921 tctcttaaaa ttaaccaagc cgattgttta tctaataatt gttctaaatt tttcatttgc
    43981 aaaatttcct tagaatttga ccgcttgcga aaaccataca aatgtaggat taatagtcaa
    44041 agaatttttc ttctactttc tcaatctgaa taattaattt tttaaagtaa cggagcttgt
    44101 cggttaacgc attggctttt tgccagtcgg ctgtatttaa cgtttgttca agctgttcta
    44161 atactgcttt ttgttccgct ttaatctgtt ttaaaaagac ggtcagttcg gtttcgtctt
    44221 tacgatgctc aatggtttcc agtttttcgt gccattcgag ttgctgcatt aagaaatcca
    44281 catctcgcat acttttttct tcgagatttt tggcttcacc tgtatggatt tcaatcatcg
    44341 cttctgcccg taaaatcgga tctttaagaa tattgagtgc ctcattgaca tcagcagatt
    44401 tttgcaccgc ttctaactgt tcagcacttg atttgtgggc aaagttatct gggtgaagct
    44461 gtttctgtaa agcaagataa cgctcagaaa gctgggcgtt atcaagttga aattgaacgg
    44521 gtaaatcaaa taaagtaaaa gggttattca ttacagttcc ttacacacta aaactttcgc
    44581 cacagccgca ttcgttttta acattcgggt tggtgtattt aaagccttcg tttaagcctt
    44641 ctttcacaaa atccagctgc gtaccgttta agtaagtcag gcttttttca tcgacaataa
    44701 cttttacgcc gtgttgttcg aaaacctgat cgtcttcatt taatacatca acaaattcaa
    44761 gcacataggc aagccccgaa caaccggaag ttttcacacc aagccttagc ccgatacctt
    44821 tacctctgtt ttctaagaat gtacgaactc ggtttgcggc agcttctgtt aaggtaatgc
    44881 tcatagtttt ccttatgtga atggattaaa tcaagataga aatagcaaat gcaagcggtt
    44941 aaattttgca aattttttgc aaaaatcgac cgcttgcatt gattgtatca tattacttag
    45001 cttgtttctc tttgtagtct gcaattgcag ctttgattgc atcttctgct aagattgaac
    45061 agtgaacttt taccggtggt aattctaact cttcagcgat atcactgttt ttaatcgcac
    45121 ctgcttcctc taatgattta ccttttaccc attcggtaat gagagaactt gatgcgattg
    45181 cagaaccaca accgtatgct ttaaagcgtg catcttcaat gataccttga tcgttcactt
    45241 taatttgtaa acgtaaaata tcaccacaag ccggtgctcc caccatacct gtaccggtgt
    45301 ctgatgcttc tttatcaaaa gtccctacgt tgcgtgggtt ttcgtaatga tcaattactt
    45361 tttcgctata tgccattttt gtattccttt tatttcctat ttccggcgaa acctatttcg
    45421 cccctacatt caatttacta tttgtgatga aagcttattt tgtaggggca aatattattt
    45481 gcccttgcaa aatttttctg aattagtggt gattccattc gattttagac aaatctacac
    45541 cttctttgaa catttcccaa agaggcgaaa gctcacgcaa tttcacgata gcttttttca
    45601 cgatttcaat agtatgatcg atttcttctt cagtcgtcca acgacctaaa gtgaaacgga
    45661 ttgagctgtg agctaattca tcgtcacgac cgattgcacg taacacataa gatggctcta
    45721 aacttgcaga agtacaagct gaacctgatg atactgcgat atcacgtaat gacatcatta
    45781 acgattcgcc ctcaacaaag ttaaagctga tgtttaggtt tgaaccgata cgtttgcctt
    45841 cttccattga accgtttaca tatacttctt caatatcttt aaagccgtta tataaacgat
    45901 cacgtaacac ggttaaacga gccatttccg ttgccatttc ttctttagcg atacgatatg
    45961 cttcacccat acctacaatt tggtgaacag gcaatgtacc cgaacgcata ccacgctcgt
    46021 gaccgccacc gtgaataatt gcttctaaac gcacacgtgg tttacgacaa acatatagac
    46081 cgccgatacc ttttggtccg tataatttgt ggcttgagaa agacattaaa tcaacattta
    46141 agtcttgtac attcacttcg attttaccaa cagactgggt cgcatcaacg tggaaaatga
    46201 ttttcttcgc acggcagatt gcaccgatct ctttaatcgg ttggattacg cccatttcgt
    46261 tatttacgtg cattaccgaa actaaaattg tgtccggacg aatagccgct tttaatttag
    46321 ctaaatctaa aataccgtct tcttcagggt ctaagtaagt cacttcaaaa ccttcacgct
    46381 ctaactgacg gcaagtatct aatactgctt tatgttcggt tttaacagtg ataatgtgtt
    46441 tacctttggt ttgatagaaa tgagccgcac ctttgatggc taagttatcc gactctgttg
    46501 caccagaagt gaaaacgatt tcacggctgt ctgcaccgat taaatcggca atttggttac
    46561 gagccacatc aaccgcttct tccgcttccc aaccaaattt gtgtgagcga gaagcagggt
    46621 taccaaagat accgtctttg gtcatatatt ccatcatttt tttcgcaaca cgttcgtcca
    46681 ttggtgtggt tgctgcgtaa tccaaataaa ttggtaattt cattgttact cctagttaaa
    46741 acaacccaag cggtcatttt tgataaaaaa tttgcaatta gtgatgatga ggatgatcgt
    46801 gacaatgctc tttttcacaa tcgtgatcag cgtgttctgc ggctaaatcc gcaactgaaa
    46861 ttgtccctaa aaactgctca atttgatctt ctaaccgctc ccacaaagaa tgcgttaaac
    46921 actgagaatc tttacggcaa ttaccactac ctttacattt ggtgacatca atattttcat
    46981 tcacggcgga gatgatcata cccacgctaa tctcttccgg ttttttacct aattgatacc
    47041 caccacccgg tccacgaacg ctctgcacga taccgtcacg gcgaagttga gcaaacagtt
    47101 gctctaaata agacagtgaa atcgcttggc gatctgaaat atcggctaag ctaaccggct
    47161 ctagttttcc gtgtaaagca atatctaaaa tggcggttac tgcataacgc ccttttgagg
    47221 taagcttcat aaaatatcct cattgattat ccagaaaatc ttctgaaaca gttgctaaat
    47281 tttgatatac cccaccaatt tagtcaatca tttaggacta attagtgtaa aaggttttat
    47341 atattaaaac agattaaaac aataacgctg attatacgaa aaaagagccg aaaataccaa
    47401 aataccaaag ataaacagtt catagaccgc acaatttcac tcttatttgc tcctaactga
    47461 tcggaacagt tgaaatttaa ttgatagaga gtacttcgac acataaaatc tgcactttct
    47521 tcttataact tttatgaatt attcattcta aaatatatat tttttaagag atttcctatg
    47581 aaaatgaaaa actttagcaa aactttaatc gcttcattag tagcattcag ctcagccggt
    47641 gcatctgctg ccgcattcca attagctgaa atttcaacat ccggtttagg tagagcttac
    47701 gcaggtaatg ccgctgtagc cgataacgct tctgttgttg caaccaaccc tgcgttaatg
    47761 acgttattta aacaaaccga aatttctgcc ggtggtattt tcgtagatgc tgatgtaaat
    47821 attgatggaa agctcaatgc tacaggtgca gatgcctcac ataaaaatgt cattcctacc
    47881 gctattgttc caaatgctta tatcgtttca cctatcaatg agcgcctttc tattggtggt
    47941 ggcgtaaatg taaattatgg tttgatatct gaatttgacc ctaactattc tgctggtttc
    48001 ttaggtggtc gtacatcatt aagtgctgtg aatgttaatt ttagtgcggc ttatgattta
    48061 ggttatggtt ttgtattagg ggcaggtttg aatgcagtac acgcaaaagc agaattagga
    48121 cgtttcttag gtgccggtgc ggcacgtttt gctccagcta aacaattttt ggaagacaag
    48181 aaagtacaag tacaaagtgc aattcaaggt ttaaaagctc ttgaggcagg tatcgcagca
    48241 ggtaataccg ctcttgaagc tcaaaaaaca caagtattag ctcaagtaaa cggtttatta
    48301 aaatctcaaa atttaaacca aaccatcacg aattctgctc aattagaacg tttggttaat
    48361 ggcattcaaa ataccaatgc aagttcaaca attcataaac ttaaaggcga taaatgggca
    48421 ttgggttgga atgttggctt ggtttataat atcaatgaaa gaaaccgttt aggttttact
    48481 taccactctg aaattgatct taaatttaaa ggcaaatatt ctaataccgt tcctgtagtt
    48541 attcctaaga tggtagatac cattaccggc ggtgcagaga ttgatggtac actaagatta
    48601 cgcctaccgg cattctggga agtgtcaggt taccacaaac taaccgataa acttgctgca
    48661 caatacagct ataaacgcac agactggagc aaattccaaa gcctagaagc ttatgatatg
    48721 caaggtaacc gcctattgca caaaaccgag aactttaatg actctcaacg tattgcatta
    48781 ggcttgtctt atgatgtgaa tgaaatgcta acattgcgta caggtgtagc atttgatgaa
    48841 agtgcaagtg taactcatcc atcaatctca attccggaca ctgatcgtgc ttggtattct
    48901 gtaggtgcaa ccgtgcgttt cacgccaaac ctatctgctg atgtaggtta ctcacatctt
    48961 cgtggtagca acctgacctt ctctgaagac ggtttagcca cattcaagag caaagctcgt
    49021 gcaaatcttt acggtttaaa cgtaaactac aaattctaat ttgatgaatt aaggtttaaa
    49081 gaggtgtaaa atcaattaca cctctttttt tattggcata aaaacatgaa atctcaaatt
    49141 tactacgatt actacccaag ccctatcggg caattattgc ttttagccac agaagaaggc
    49201 ttgatttaca ttgagctagg gcaagagcaa caaaccacct cgcttgataa tttcctttcc
    49261 gctcaagctg caagtggtca gattttagaa attttttgca aaaccaaaga aattttagac
    49321 cgctattttg ccggcgagcc attggatttt cagcagttgg attttcttgc cccgcaaggc
    49381 actccattcc agcaatcagt ttggaaaatt ttgcgtgaaa tcccttacgg gcaaactact
    49441 acttatggcg aaattgccac ccaactaggc aagccaagtg cgatgcgagc cgtcggcggt
    49501 gcagttgggc gaaacccgat ttcgatttta gtgccttgcc atagggtatt agggaaaaat
    49561 caagccttaa ccggattcgg tggtggcttg ccgaataagc gttttctatt acagctggaa
    49621 gatattacct ataaagataa aggcattgaa tttgtaaaac cgaaaaccaa gaaatggtaa
    49681 atacgttttg atagcacaag cgtccacgct tgtgcttcag atattaaaat tggtacaagc
    49741 gtggacgctt gcaccatcgt aacatttcaa atttactaaa tgtccccctt gcttctaaca
    49801 tattccttag ctaattgcaa atccatctgc catttggaac aaatcatttc cacccagtgt
    49861 gcaatatttt gctcccactg ctcgccgtta tcggattgag caagctgagc agtttgttgt
    49921 agctgatgta atccgactga tgctaatgct cccttaatct tatgagcctc gtccgccgtt
    49981 aatttacggc tttccacaga atttgtggtt tgccaatcac ttaaataacg ctgtaaattt
    50041 gccaaataag tgggcattaa atcagcaaat aaatccatat tggcaagcac attctctttc
    50101 cccattaccc caatcaattc attaagaatt gtaatgctat aaggtaaatt ctcagaacct
    50161 ctctctacaa gcggctgttt ttcttcattt ttttgcaaaa aatcatctcc aaaatgttgg
    50221 ttgagacaac gcattaattc atctaaagag agcggtttgt gtagcacatc gtccatacct
    50281 tgtgcttggt actcctgccg gctttggatc acatttgccg tcaaagcaac caacagcggt
    50341 aaatagtcaa tttcctcgtt ttcatattgc aatcttaatt gctgtgcaat ttcaaaaccg
    50401 gtggtatccg gcagttgaat atcaagtaaa atcagatcgt agctgttctg tgcaaaatgt
    50461 tggaacgttt cttcaccact cattgctaca tcaaccacgc aaccgatttt ctccaacatt
    50521 gctcgggcaa ccaccacatt catttcaatg tcttctacca atagcacctt aagggaatgt
    50581 tgctgaatag caaatatcgg cttggtttcc gtgctttctg cctgcacagt gagggtaaaa
    50641 gttgagcctt gattttcttg gcttgccacc accaaatcac cgcccattag cttcgcaatg
    50701 cgtttcgaga ccgataagcc aatcccgcta ccttgcgctt ttttctcttt agagttttcc
    50761 gcttggtaga acatatcaaa aattttgcgt tgatcttttt ccggaatacc gatgcctgaa
    50821 tccgtcaccg caaagctaaa ctgttcttta tctaattggt aaatttccag caaaattcga
    50881 ccgcttgcag gggtaaattt caccgcattg ctgatcaaat tccataaaat ttggctcaag
    50941 cgtgcgtggt cgatatagat atacttcggc aaatcaggcg gatacttcac ttcaaaatta
    51001 atctgcttat tgcccgccat aaaatgccca aaattagcaa tatcgtgaat gagttgttca
    51061 aattccacct cttttcttac caattcaatt cggctactat caattttttc taaatcaata
    51121 atatcgccaa aaatatgagc caatgcagtg gcactcatat tgatagtttt tagatattct
    51181 tgctgttttt cggtggtttt atcatctaac aaaatttggc tcaagccgat aattccatta
    51241 aggggagtac gaagttcgtg actaatggtc gccattaaaa tgcttttatc acggctgttt
    51301 ttttctaatt tagcgttaag ataggcatct tgttcccgga gttcttccaa atctcttact
    51361 gctttttcta atttcaagcg agatatttca agtttttcca ccaataacgt gaagaaataa
    51421 agcacaaaag gcgcagaaat taaaccaaaa gcgatggagc gtaaaatatc caccacgact
    51481 actcgcccaa taaaaagata ggttaagcta ctttgaatta aaattgccga aagagcgacc
    51541 ataaaaaagc ctaaaagaga agcttttaac cgccctagct ttaataccca attaatatat
    51601 ttttggctaa attttttaac ttgcatacga cctcaaaaca aaaagcagac catcagatct
    51661 gctttatttt actctatttt ttactttttg cctttgacta gctcatcagc ccataggaat
    51721 aattcattta catcataatc acctgaatgg ggtacattcc acggggtttc ataattaaca
    51781 tttttgcctg ccatttgcag ttttattgct aagattgcag aaattgccag cgaggtatct
    51841 ctatctgccg ttcccaccct aatccgccaa tgttccgcag catttgggtt atccaaatag
    51901 tgcatcgggt tcatccgttt cacgatttgc ggatcggcta aattgagatc tttattggca
    51961 gaacgttccg cactataaag cgtaaaatgg cggttatttt ccgtgtctgt gccaaataaa
    52021 ttattctcgc ccgaacttcc atttaacgca tcaaatgcag gtggtgattt catccgttta
    52081 tctgaaaaca cataaccaaa ccagtccaca ctgctgactt tgcctttctc aaatgaaagc
    52141 caactaattt gttgaaaggc tttttgctct tcgctatctt gaactaaacc tctataggct
    52201 ttatttgcag ccgtttttac cacttcagat aaataatctt taaacgagcc gttgcctttt
    52261 ggatcaagtg ttaaaggatt gcctttctca tcaaccaaat gcaaactgtt caaataagtc
    52321 ggaaagcgtt ctgctaattc attagaaact ttaatttccg cctcatttaa cgagccttca
    52381 attttcggct ttggttttgg ttttgagcgg tcgttaaact ctgctgcatt taatcgactc
    52441 atatcgattc ggctatattc attgacacca ttaaattgcc attcatacgc catatccgca
    52501 ttttcaaggt tggtaattgg gcaataggca gaaactgcaa aaattttgtc actggcattc
    52561 aatgcccccg cctctttcaa ataaggctca tagtcgtaat gatcagctga agctcctaac
    52621 aaagtagata atgctcctcc ggcactggtg ccgttagaga taattttatt tgcatcgccc
    52681 ggcatttttt catcattagc atgcagatac cgcactgctg attttgaatc aactacgtag
    52741 aatcattcaa ataacattca caaatacata gttattatga tgtatccagc caatacatcc
    52801 actgggattt tccatccgaa tttcggacaa tcctgatctt acacattctt tcagatttgt
    52861 aaagtctgaa aatcattttt atgcttgatt ttacttgaaa gtatctatct tggtctgaca
    52921 ttattccgtc ttattacagc tctttgaaca taaaatcgac tcatgtttgt ttacattcta
    52981 aatatatttt ctgtttaaat cgcaaagagt ttatcaaaag atgaagtcga tcatctcatc
    53041 gcgtctgcct ggttca
//
LOCUS       DANLXQ010000002        43744 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.002, whole genome shotgun sequence.
ACCESSION   DANLXQ010000002 DANLXQ010000000
VERSION     DANLXQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 43744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43744
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.002"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..399)
                     /gene="fur"
                     /locus_tag="PXD94_000060"
     CDS             complement(<1..399)
                     /gene="fur"
                     /locus_tag="PXD94_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL6284974.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEFKDPDMERRQREISEKHGMELTNHSLYLY"
     gene            complement(435..959)
                     /gene="fldA"
                     /locus_tag="PXD94_000061"
     CDS             complement(435..959)
                     /gene="fldA"
                     /locus_tag="PXD94_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL6284975.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            complement(965..1240)
                     /gene="ybfE"
                     /locus_tag="PXD94_000062"
     CDS             complement(965..1240)
                     /gene="ybfE"
                     /locus_tag="PXD94_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL6284976.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSNLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            1481..2467
                     /locus_tag="PXD94_000063"
     CDS             1481..2467
                     /locus_tag="PXD94_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL6284977.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANALSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAVKLLKLVLAY"
     gene            2564..3859
                     /locus_tag="PXD94_000064"
     CDS             2564..3859
                     /locus_tag="PXD94_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL6284978.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGMG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGMIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVVVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            3979..4347
                     /locus_tag="PXD94_000065"
     CDS             3979..4347
                     /locus_tag="PXD94_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL6284979.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            4347..5540
                     /locus_tag="PXD94_000066"
     CDS             4347..5540
                     /locus_tag="PXD94_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL6284980.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVIATG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(5611..5829)
                     /gene="infA"
                     /locus_tag="PXD94_000067"
     CDS             complement(5611..5829)
                     /gene="infA"
                     /locus_tag="PXD94_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL6284981.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(5939..6598)
                     /locus_tag="PXD94_000068"
     CDS             complement(5939..6598)
                     /locus_tag="PXD94_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL6284982.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            6684..7547
                     /gene="serB"
                     /locus_tag="PXD94_000069"
     CDS             6684..7547
                     /gene="serB"
                     /locus_tag="PXD94_000069"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL6284983.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDDIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLNFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLQ
                     NLATTFNIPPNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            7563..8054
                     /locus_tag="PXD94_000070"
     CDS             7563..8054
                     /locus_tag="PXD94_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL6284984.1"
                     /translation="MPSFDIVSEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            8139..8315
                     /locus_tag="PXD94_000071"
     CDS             8139..8315
                     /locus_tag="PXD94_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL6284985.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(8363..8659)
                     /locus_tag="PXD94_000072"
     CDS             complement(8363..8659)
                     /locus_tag="PXD94_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6284986.1"
                     /translation="MKKHHIIILTLASASVVACSSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(8736..9704)
                     /gene="mdh"
                     /locus_tag="PXD94_000073"
     CDS             complement(8736..9704)
                     /gene="mdh"
                     /locus_tag="PXD94_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL6284987.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAKDVNVKYVRVPVIGGHSGTTILPLLSQATVNGLKLEFTQEQIEQLTHRIQNAG
                     TEVVEAKAGGGSATLSMAQAGAEFALGLVRALIGEDVIRYAYVDNANGETSPAFFAYP
                     IRLGTNGVEKVLPIGNLSEFEKDQLEQLIPVLNDEIQLGQTFNKNA"
     gene            9900..10361
                     /gene="argR"
                     /locus_tag="PXD94_000074"
     CDS             9900..10361
                     /gene="argR"
                     /locus_tag="PXD94_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL6284988.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNVQIEKLVKNVTQLFETSL"
     gene            10411..11307
                     /locus_tag="PXD94_000075"
     CDS             10411..11307
                     /locus_tag="PXD94_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL6284989.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSDDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            11352..13550
                     /gene="uvrD"
                     /locus_tag="PXD94_000076"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11352..13550
                     /gene="uvrD"
                     /locus_tag="PXD94_000076"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL6284990.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHYNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKSTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
     gene            complement(13697..13771)
                     /locus_tag="PXD94_000077"
     tRNA            complement(13697..13771)
                     /locus_tag="PXD94_000077"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13737..13739),aa:Gln,seq:ttg)
     gene            complement(13826..13900)
                     /locus_tag="PXD94_000078"
     tRNA            complement(13826..13900)
                     /locus_tag="PXD94_000078"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13866..13868),aa:Gln,seq:ttg)
     gene            complement(13926..14010)
                     /locus_tag="PXD94_000079"
     tRNA            complement(13926..14010)
                     /locus_tag="PXD94_000079"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13974..13976),aa:Leu,seq:tag)
     gene            complement(14013..14089)
                     /locus_tag="PXD94_000080"
     tRNA            complement(14013..14089)
                     /locus_tag="PXD94_000080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14053..14055),aa:Met,seq:cat)
     gene            complement(14256..15080)
                     /gene="aroE"
                     /locus_tag="PXD94_000081"
     CDS             complement(14256..15080)
                     /gene="aroE"
                     /locus_tag="PXD94_000081"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL6284991.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQLFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            complement(15087..15641)
                     /locus_tag="PXD94_000082"
     CDS             complement(15087..15641)
                     /locus_tag="PXD94_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL6284992.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            complement(15634..16170)
                     /locus_tag="PXD94_000083"
     CDS             complement(15634..16170)
                     /locus_tag="PXD94_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL6284993.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            complement(16180..16491)
                     /locus_tag="PXD94_000084"
     CDS             complement(16180..16491)
                     /locus_tag="PXD94_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL6284994.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            complement(16484..16960)
                     /gene="recX"
                     /locus_tag="PXD94_000085"
     CDS             complement(16484..16960)
                     /gene="recX"
                     /locus_tag="PXD94_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL6284995.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            complement(17019..18125)
                     /gene="recA"
                     /locus_tag="PXD94_000086"
     CDS             complement(17019..18125)
                     /gene="recA"
                     /locus_tag="PXD94_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL6284996.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
     gene            complement(18226..18846)
                     /locus_tag="PXD94_000087"
     CDS             complement(18226..18846)
                     /locus_tag="PXD94_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL6284997.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            complement(18824..19798)
                     /locus_tag="PXD94_000088"
     CDS             complement(18824..19798)
                     /locus_tag="PXD94_000088"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL6284998.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQQKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVEKPTKAFELSANPIPFSEYQTGFELVKKEIQAGNSYLLNLSYPTAI
                     ETNYSLAEIFAASKAKYKCYLQGRFVCFSPECFVRIENNRIFSYPMKGTINANEENAE
                     QKLMASEKEFTEHNTIVDLIRNDLALVAKNIEVTKYRYVEKVETHRGAILQTSSEICG
                     ELGENWQENIGSILAKLLPAGSISGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESL
                     ESAVAIRYIAQTSNGLVFHSGGGITSQSVLTDEYNELLEKVYVPISAI"
     gene            19908..21515
                     /gene="rmuC"
                     /locus_tag="PXD94_000089"
     CDS             19908..21515
                     /gene="rmuC"
                     /locus_tag="PXD94_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL6284999.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRNAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEVDTIKLEMVSRELLEE"
     gene            21578..22162
                     /locus_tag="PXD94_000090"
     CDS             21578..22162
                     /locus_tag="PXD94_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL6285000.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            22259..23341
                     /gene="prfA"
                     /locus_tag="PXD94_000091"
     CDS             22259..23341
                     /gene="prfA"
                     /locus_tag="PXD94_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL6285001.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSEQN"
     gene            23453..24055
                     /locus_tag="PXD94_000092"
     CDS             23453..24055
                     /locus_tag="PXD94_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285002.1"
                     /translation="MRKLGLLAVFTLVLSACSNSTKIEQTILSKQDIVGSWVCAIKYD
                     DLKVSTLDFSEFKANGEMTNFGDISDENFEPIKFTYVTGDKGKWDLKGNRLVIDYDLS
                     ERKVKRTTPKKFLEFLKQEERKASKTAKKLLKYEQGIFNILSDQKNTENSKIILDILK
                     FGHSRMAIQQNMGDKVYSGGCVTADKAEEYLDILKNRNSK"
     gene            24055..24921
                     /gene="prmC"
                     /locus_tag="PXD94_000093"
     CDS             24055..24921
                     /gene="prmC"
                     /locus_tag="PXD94_000093"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL6285003.1"
                     /translation="MDYREWLTFAEQKLLENSGADPYLNAKFDANLLLQAVTKRSKSA
                     IFAFSETLLSESELAELAEKLARRAKGEPMAYILGYREFWSLPLKVSPATLIPRPDTE
                     RLVELALEYANKRLGNQKMLQILDLGTGTGAIALALASELGEKAQIIGVDFQNEAVQL
                     AEENRRNLGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDESDENLTVGDVRFEPLTA
                     LVADNKGLSDLQKIIENAPLHLKPQGALMLEHGWKQGNLVRDLFDLHLWEQVETAQDY
                     AGNDRVTKAILK"
     gene            24930..25796
                     /locus_tag="PXD94_000094"
     CDS             24930..25796
                     /locus_tag="PXD94_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL6285004.1"
                     /translation="MDDIIEELLKELEDPKVTISETELKKFLYREILRLTTLIDKNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLHLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVALYLSAVLDLPLYPVNFPTQLVLRAEIHQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESVVH"
     gene            25886..26740
                     /gene="kdsA"
                     /locus_tag="PXD94_000095"
     CDS             25886..26740
                     /gene="kdsA"
                     /locus_tag="PXD94_000095"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL6285005.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DQVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGLAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            26961..27908
                     /locus_tag="PXD94_000096"
     CDS             26961..27908
                     /locus_tag="PXD94_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL6285006.1"
                     /translation="MLNIVFLDRTGIPATHDIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSSVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVATGM"
     gene            28006..29178
                     /locus_tag="PXD94_000097"
     CDS             28006..29178
                     /locus_tag="PXD94_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL6285007.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIILLLNPSIHLPTLISGTQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            29195..29878
                     /gene="lolD"
                     /locus_tag="PXD94_000098"
     CDS             29195..29878
                     /gene="lolD"
                     /locus_tag="PXD94_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL6285008.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAANRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            29875..31140
                     /gene="lolE"
                     /locus_tag="PXD94_000099"
     CDS             29875..31140
                     /gene="lolE"
                     /locus_tag="PXD94_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL6285009.1"
                     /translation="MSTPFFISWRYQRGKQKNKLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALIKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(31215..33146)
                     /gene="thrS"
                     /locus_tag="PXD94_000100"
     CDS             complement(31215..33146)
                     /gene="thrS"
                     /locus_tag="PXD94_000100"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL6285010.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSTLEIITAKDEDGLEIIRHSTAHLLGHSIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVISCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEKGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLAASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(33313..34662)
                     /locus_tag="PXD94_000101"
     CDS             complement(33313..34662)
                     /locus_tag="PXD94_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6285011.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIGLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLIISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEVVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEGAAENSKV"
     gene            complement(34937..35428)
                     /locus_tag="PXD94_000102"
     CDS             complement(34937..35428)
                     /locus_tag="PXD94_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL6285012.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSLYQKAQYYKRAYI
                     HLF"
     gene            35509..36276
                     /locus_tag="PXD94_000103"
     CDS             35509..36276
                     /locus_tag="PXD94_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL6285013.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYY
                     HGGCCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVN
                     CCENLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(36267..37061)
                     /locus_tag="PXD94_000104"
     CDS             complement(36267..37061)
                     /locus_tag="PXD94_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL6285014.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAKQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(37122..38708)
                     /locus_tag="PXD94_000105"
     CDS             complement(37122..38708)
                     /locus_tag="PXD94_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL6285015.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRATPAYAMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(38894..40747)
                     /gene="dxs"
                     /locus_tag="PXD94_000106"
     CDS             complement(38894..40747)
                     /gene="dxs"
                     /locus_tag="PXD94_000106"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL6285016.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRKMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQRLEVGKGNIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(40792..41604)
                     /gene="suhB"
                     /locus_tag="PXD94_000107"
     CDS             complement(40792..41604)
                     /gene="suhB"
                     /locus_tag="PXD94_000107"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL6285017.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            41785..42819
                     /locus_tag="PXD94_000108"
     CDS             41785..42819
                     /locus_tag="PXD94_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL6285018.1"
                     /translation="MQFKENNQPRFKHREERRSDECSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPESIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSAAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            42829..43713
                     /locus_tag="PXD94_000109"
     CDS             42829..43713
                     /locus_tag="PXD94_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL6285019.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YSYFTVLGSKLGWASKLF"
BASE COUNT        13071 a         8734 c         9306 g        12633 t
ORIGIN      
        1 atacagatat aaactgtggt tagtcaattc cattccgtgt ttttcactga tttcacgttg
       61 gcgacgctcc atatcaggat ctttaaattc gaacacctta ccacaatcca tacaaataat
      121 atggtcgtgt tcgtggttca tatttaattc gaatactgct ttattcgcat cgaaattatg
      181 gcgggttaaa atgccggctt cttcaaattg gtttaagaca cgataaaccg ttgctaaacc
      241 aatgtctgag cctttttcta acagaatctt ataaatatct tctgctgcaa aatgttgcat
      301 ttcatcacga atatcttgca ttaatgcaag gatagtcaaa cgaggctcgg ttacttttaa
      361 gcctacctct tttaaacgtt taatatttgc ttcagacata tatgccactt atttatcttt
      421 atccaaaata gttattatgc gagttggtct aagcacattt cttctttcaa ctgctcgcac
      481 catttattaa cacgttcagt ggttaattca ggctgacgat cttcatcaat acataagccg
      541 acaaaagtat catcatcaac taaggcctga gaaacctcga aagtgtaacc ggcagttggc
      601 caatgaccaa caatcacacc accatttggc tctaccacat cacgcacagt acccattgca
      661 tcacagaaat attctgcata atcttcctga tcaccgcaac caaaaattgc aacgactttg
      721 ccgttaaaat caatctcttt tagggtaggc ataaaatcgt cccaatcggc ttgcgattca
      781 ccataatacc aagttgggat cccaaacatc agaaaatcgt atgcttcaat atcttctttt
      841 gtgcttttgg caatgtctcg aatatcaatt aattcagccc ctaatttttt ctgaatcatt
      901 tttgaaatat tttctgtatt gcctgtatcg ctaccgtaaa ataaacctac aactgccatt
      961 gctactacct atttgtgaga taattattta gaattgtttc aattaactgc ccgcgtgaaa
     1021 caccttgttc agacgcctgt tcctctaata actttactaa attcgaatgc agttttaatt
     1081 ctacccgttt taaaccggaa gatttatctc gttttagttg gttacgttta ttgattcgaa
     1141 gttgttgttc tctatcaaga ggatttgtac gaggacgccc aacttttggt acagtcgcaa
     1201 acagatctaa ggttatacaa tctgaatctt gttttgccat tttgttttac tattgggctt
     1261 tagctaatcc tcagttttaa tgagaatgat aagctacttt taaagtgtga actatatcaa
     1321 aagttcaatc atttgaaaag taaattacag gcaaaacaag cggtgttttt aggtttaaaa
     1381 tttgcaaaaa ccataaatta cacattatga gttatgattt ttctgaagcg tgaaattagc
     1441 ttataatgaa accgaataac gttatgaaaa aggagatagt gtgtcgaata tccatttagc
     1501 gattgccgct gattttacgc tggcagaaaa attacttgaa gcattagcaa atcaagattt
     1561 agggattgaa aatatttcag ccgttgagct tgagccgttt ggtgaagagc agaatttacg
     1621 ctttggggca aaagcagtcg cacaatatgc gttagatgaa gtaaattggg cggaatttag
     1681 ccatgttcta tttgccggca aaatggcaca agcggaagtg ttggcagaag cggtgaaagc
     1741 cggctgtgtg attttagatt tatacggcat taccgcactc atcggcgatg taccggtagt
     1801 tgtgccaagt gtaaatgatg aaaatgtcgc aaatttgcgt gagcgaaata tcgttgcttt
     1861 agccaatccg caaatttcac aactggcgtt agccttaaag ccattgttga acgagccgtt
     1921 aaaacaggtg tgggtaacct cacttttacc gtctgcctat tttggcgatg aaaaagtcaa
     1981 ggagcttgcc ggacaaactg cccgtttgtt aaacggcatt ccgtttgatg aaaatgcgga
     2041 acgcattgcc tttgatgttg tgccggctaa tgtgcaagga gaagagaaaa gtctgccgtt
     2101 ctcaaaagcc tttgaactgc aaattgcaaa agttttacca aatttgaccg cttgtactac
     2161 cttccacagc gtgcaagtgc cggtctttta tggggtttcc caaatggtgt cggtgcagtc
     2221 tgaatatcag ttagatgcta atgcgttaag tgaagcttgg aaagccgaaa attggctgaa
     2281 ttttaatgaa aattcagtga ttacagcggt gaaaaatggc gaaaacgaag agcaaaatgc
     2341 gttagaaatc agtcagttat tggcgaaatc cgaacaggaa gtgcagttct ggacggtcgc
     2401 cgatgaacag cgtttcagtc tggcattttt agcggtgaaa ttactaaaat tagttttagc
     2461 gtattaagtg cttgcaaaag tatttattcc gtgataaaaa tcgtcaacgc ccgagcaacc
     2521 gattgcttgg gcgttgtcat tttgtttaaa caccgaaggt cttatgttag aaaaattatt
     2581 tcagctcaaa gcaaaaggca gtaatgtaaa aaccgaaatt atcgccggta ttactacctt
     2641 tttcacaatg gtttatattg ttttcgtcaa tccgtctatt ttaggcgtgg cgggaatgga
     2701 cacccaagta gtgttcgtga ctacttgttt aattgccgct tttggtacta tcgcaatggg
     2761 gttattcagt aatttaccga ttgctcttgc accggcaatg ggcttaaatg ccttttttgc
     2821 attcgtagtg gtgcaaaaat taggttactc gtggcaagtg ggtatggggg cgattttctt
     2881 aggctctgtc ggcttattcc tattaactgt attacaagtt cgttactggt ttatgtctgc
     2941 gattccactt ggtttacgtg taggtattgg tgccggtatc ggtttattca ttgccctaat
     3001 cggttttaaa aatatgggct tggtggtgcc aaatccggca acgcttgtgg cactaggtga
     3061 tttgcacgac ccgaaagtgt taatggggat tttaggcttt ttcatcattg ttgtattagc
     3121 ggcgaaaggt attcactcag gcgtgttaat ttcaattgtg attgttaccg ctcttgcctt
     3181 agcctttgac ccggcggtga gctttaacgg cataatgtct atgccaccaa gccttggggc
     3241 agttgtgggg caggttgata ttgccggtgc ttttgatgtt gccttactcg ggatgatttt
     3301 ctcctttatg ttggtaaacc tgtttgactc ttccggcacc ttaattgcgg tgactaccaa
     3361 agcaggcttt gcggatgaac aagggcgttt cccgaaaatg aaacaagctc tgctagtaga
     3421 tagtaccgct gcaatggcag gctcttatat gggaacttca gcgatttcta cctatattga
     3481 aagtggctcc ggcgtttcag ttggcggtcg taccggctta actgcggtgg tagttgggat
     3541 cttattccta ttaaccattt tcttctcacc acttgcaggg ttagtacctg cctatgcaac
     3601 tgcgggggca ttggtatttg taggaatttt aatggcatct agcttaattg aagtaaaatg
     3661 ggacgattta actgaagcta cgccggcatt tattactacc gcaatgatgc cgtttactta
     3721 ctccatcact gaagggattg cattcggctt tatcagctac tgcgtaatga aagcctttac
     3781 agggcgttgg aaagaaatca acctttctgt tttagtggtt tcacttctct ttattgctaa
     3841 atttgtgtgg gtgggttaag ctctaccccc ctctccctga tttttcttct aaacgaagaa
     3901 aaatctatcc ctctcccaca aaggggagag ggtatttgat tcattatcta tgtgtttcgt
     3961 gtattccgag gtaaaacaat gtctaattca gttcaaaaac ttctttttat catcaattct
     4021 gctccttacg gcaatgaaac gtttttcagt gcgttgcgtt tggcattaca aattcaagaa
     4081 caacataaag ccaatacgga aattaaactt ttcttaatgt cggatgcagt caccgcaggg
     4141 ctggcaaagc aaaatccggc agagggttat catattcagc aaatgctgga aattttaacc
     4201 gcccaagggg caaacgtaaa attatgcaaa acctgtacta atgctcgagg gattacagat
     4261 ttgcctttag ccgatggggt agagattggc acattaattg aactggcaga ttggacaatt
     4321 caagcagata aggtactgaa tttttaatgc aaaatattga tgttgtggtg attggtgccg
     4381 gtgcggcagg gctgttttgt gctgcccaac tggggcaagc tggcaaaaaa gtggtggtgt
     4441 tagacagcgg taagaaagtc ggacgtaaaa ttttaatgtc tggcggtggt ttttgcaatt
     4501 ttacgaattt agacgtaagt gccggaaatt accttagcca aaaccgccat tttgtgaaat
     4561 ccgccctttc acgttatacc caatgggatt ttatttcgct tgtcagccac tatggcatta
     4621 gctatcacga aaaagagcta gggcagttgt tttgtgatga aagctcgcaa caaattgtcg
     4681 atttactaca agcagaatgc aacaaaggca aggttgagat tctgttacgg caagcggtca
     4741 tttctgtgga aaaagttgca aatttttttg aaatttcgac cgcttgtggc aagttccaat
     4801 ccgaacactt agtgattgca accggagggc tttcaatgcc ggctctcggg gcaagccctt
     4861 ttggctatca acttgccgag cagtttggta ttcctgttgt gccaccacgt gcaagccttg
     4921 tgccttttac ttggaaagag agcgataaat attttgccga gctttccggc attgcactgc
     4981 cgattactgt ttccgccaat ggcaaaagtt ttagcaatca gatgttgttt acgcacagag
     5041 gcttatccgg cccggcaatt ttgcagattt ccaattattg ggaattgggg gaaacggtag
     5101 agattgatct gctaccttcg cagtccatta ccgatatatt gagcgaacta cgccaatctt
     5161 cgccgaaatt acagctaaaa accgtattat ctcgttattt acctaaaaaa ttggttgaag
     5221 tttggctgga acagaaattg ctgaccgata aagtgattgc cgatttgagt aaaaaagatc
     5281 tgaccttatt agagcaattt atccaccatt ggcatttctt gccaaacggc acggaaggct
     5341 atcgtacagc ggaagtaacc aaaggcggtg ttgataccga ctatatttca tctaaaacga
     5401 tggaagcgaa aaatacagca gggctttatt ttatcggcga agtgctggat gttaccggct
     5461 ggcttggtgg ttataatttc caatgggcgt ggtcatctgc ttttgcctgt gcagaaggga
     5521 ttattgaaaa aagcaaataa ctaaaaaagc aaataactaa aaaaccaaac aaaaaggcga
     5581 acacaattgt tcgccttatt ttatgctgaa ttatcttgca cggaaaataa tgcgtgcttt
     5641 gcttaaatcg tatggagtca tttcaaccgt tactttatca ccggttaaaa tacgaatgta
     5701 gtttttacgc attttgcctg aaatatgtgc ggtaacaata tgaccgtttt ctaattccac
     5761 acggaacatt gtatttggta aggtttctaa aatcgtacct tgcatctcaa tgcaatcttc
     5821 tttagccatt tattcctctt taaagtttta atttttatat gatgccattc ggcgaaaaac
     5881 ggcggatatt atacgcttat tcgctcaaat tacaatcgtt tatccatttg agtgtaaact
     5941 atttacgctt aatccaacgg cgacgtgagt gaaaacgctg ggtttgggtt tttacttgac
     6001 tcataagtgg ttttgggctt atctgtacat gatgaatttt acgacgaaaa tagtagttaa
     6061 tgctacttgc caacaatata cctgtgagta gcaaaataat tatttcccca taaagttgat
     6121 gaaattgttg ctctactcgt gctttagtag tttgcccata ttctgcatca aacgtgacgc
     6181 gtaaaaagcc aaccaacgcc tgttgagaaa atattggctc aacaatttgc tgagttttgg
     6241 cagattgctc tgaatcgagc aatactttta aatctttcgc attttggctt tgagccagta
     6301 gcacgccatt tggtgaatag atattggcat caataataaa atcttctttg gcaaaactat
     6361 ccaacgcatc aaccagctca tcggttttgg catttttaac taacaaaaac gaaaaaatat
     6421 ttgcttgctg gcgaaccaat aggtgtgaaa ggtttgatac ttgattaatg ctggccaatt
     6481 gtgagcctag cttaaattgg tttacgccca ttaaaataac cgccacgatc ccacagcaga
     6541 gtagcgtaat ccctgccagc aaaagcgttt tcgttatttt ttcacgacta atgtacatcg
     6601 aaattccctc aaaatgatgc gtgattgtag cacgcttttc tgttagatta gtgcagtaaa
     6661 tttaaacaac ctaaaataag acaatgacaa acacaatttt tatctattca aaaaccttaa
     6721 acgagcgtga aattgaacag ttttcacaac aaacggcagc aattttgctg aaacaagcgg
     6781 tctatttaga ctacaaaatt gcattttttt ccacaaattt aaccgcttgc gagctgcgtg
     6841 aaacggcaca gaaaatcaat gcggatatta gcgatctcac tattattcca accttaaacc
     6901 aaggcggttt gctattaatg gatatggatt ccaccgctat taaaattgaa tgtattgatg
     6961 atattgccaa acttgccggc accggcgagg tggtgtccgc cattactgcc agtgcaatgc
     7021 ggggcgagct ggatttcgag caaagtttac gcaagcgagt cggcacgttg gaaaatgcat
     7081 cggaaagcat tttgcaaaaa gtgcgggaaa atctaccgct tatggacggc tttgagcaga
     7141 tggttgctat tcttaaggaa cacggctgga aattagcgat tgcgtccggt ggatttgatt
     7201 acttcgccga ttacttaaaa caaaaatacg ggttgaattt tgcggtctct aaccaactgg
     7261 aaattattga cggtaaatta accggcaagg tgttaggcaa agtagtggat gctcaacata
     7321 aagccgatac cttacagaat ttagcgacaa cctttaatat tccaccaaat caatgggtgg
     7381 cagtcggcga tggggcaaat gacttaccaa tgttaaaaac cgccagttta ggcgttgctt
     7441 tgcacgctaa accaaaagtg caagcacaag ctgattttgt ggtaaacttc ggggacttaa
     7501 cggcattatg tttattactt aatgccaatc atttatttca acattaattg agaggaacaa
     7561 caatgccatc atttgatatt gtatctgaaa tcacgatgca cgaagtgcgt aatgcggtag
     7621 aaaatgccaa tcgtgtatta agcacccgct acgatttccg tggggtggaa gccgttattg
     7681 agttaaatga aaaaaatgaa agcattaagc tcacgaccga atcggatttc caattagagc
     7741 agttaattga gattttaatc ggctcttgtg taaaacgtgg gatcgagcat agttcattag
     7801 atatccctac agaaagtgag catcacggta aactctattc caaagaaatt aagctcaagc
     7861 aagggattga gtcagatatg gcgaagaaaa ttaccaagct gattaaggat tctaaaatca
     7921 aggttcaagc ccaaattcaa ggcgagcaag tgcgtgtaac gggcaaatct cgtgatgatt
     7981 tacaagccgc aattcagcta gtgaaagggg cagatttagg gcagccattc caatttaata
     8041 atttccgtga ttagcaattc ctgcaattaa tgatgacaag tggaaaaatt cagcaaaatt
     8101 tgaccgcttg cattaataat tcaacataaa aggaagagat gatgagcgag caaacagaaa
     8161 agaaaggctg gtttaaaaaa gcccttgaga aatatgatgc cttttgcgat gagctagggg
     8221 taaatcaagg tgcttgccga ggttgtgtcc ctgtggtgaa gtttgaccct gaaccggaaa
     8281 aagagcagcc agaaaaatca gaaaataaaa catagaaaaa atgcacaatg gtaagataca
     8341 ttgtgcattt tttataggat ttttaattgg cgtgatgatg ggcaaaatat tgcttaactc
     8401 cgccatcttt cttgcaagga ttctgaatat cagtagagta actaccgtta aaacaaagag
     8461 cagtagcatt cgatggttta atcttttcct gtttagttag ggctggcggt tgagtattgg
     8521 tttcactaat atagatcgtt ttaggttgag aatagtctat atttttgtcg ccatttacga
     8581 cttgaacggg ggtgttcgag ctgcaagcta caaccgatgc tgaagccaat gtaagtataa
     8641 tgatatggtg ttttttcatg ggtaatttcc gtttttatat ttttaattga gagaatgtta
     8701 aaaagggctg atttctcaac ccttaaagtt atgccttaag catttttatt aaaagtttgt
     8761 cctaactgaa tttcatcatt taatacagga attaactgct ctagttgatc tttttcaaat
     8821 tcgctgagat tgccgatagg tagaactttt tccacaccat ttgtacctaa acgaatcggg
     8881 taagcaaaga atgccggaga ggtttcgcca ttggcattat ctacataagc ataacgaatg
     8941 acatcttccc cgattaaggc tctcactaaa ccaagtgcaa attctgctcc tgcttgagcc
     9001 atagataagg ttgctgagcc accacctgct tttgcctcaa ccacttcagt accggcgttt
     9061 tggatacgat gagttaattg ctcgatttgt tcttgagtga actcaagttt taagccattt
     9121 actgttgctt gtgaaagcag tggaagaata gttgtgccgg aatgtccgcc aatcacaggc
     9181 actcttacat atttcacatt tacgtctttc gcttcagcca caaaggtatt cgctcgaata
     9241 acatctaatg ttgtaacacc gaaaagttta tttttgtcat acacaccggc ttttttcaat
     9301 acttctgccg caatcggaat aatggtgttt accgggttag taataattgc aatgcaagct
     9361 ttagggcaaa ctttcgctgc tttttcgact aagttatgca cgatagtggc attcgtattg
     9421 aataaatcgg cacgtgtcat ccccggctta cgggcaacac cagcagaaat aacgaccaca
     9481 tcggcatctt ttagtgcctc agtcggatct tcacctgcaa aaccggttac ttttacatca
     9541 gttggaatat ggcttaaatc cactgcaatt cctggagtaa caggggaaat atcgtaaaga
     9601 gataattcag atttagccgg taaattgagt tttaataata aggcgagtga ttgaccgatt
     9661 ccacctgcag cacctaaaag ggcaactttt ttactttgac acataaaatt tctcctaaat
     9721 gttatggaag tttagaccaa tttagtctaa gccaattttt tgggcgattc aaacggcaaa
     9781 tctcattttt gagacataga gcaaattttt agctgatttt tatgggtttt attgatttat
     9841 ttgcataaat aagcaagaat tatgaatatt tttaagtttt tgtgaataag agtgtgaaag
     9901 tggacaagat agatagttta tcagaagcgt ttaaatcgct gttaaaggaa gaaaaattcg
     9961 gctcgcaaag tgaaattgtt gccgcactac aagagatggg ctttgataat atcaaccaat
    10021 ccaaagtttc tcgaatgtta tcaaaatttg gtgcagtcag aacccgtaat cctaaaatgg
    10081 aaatggtgta tcagctacca gtcgaattaa gtgtaccgac tacctccagc ccacttaaaa
    10141 atttggtggt agatattgat cataatgatg cgttaattgt ggtcaaaacc agccccggtg
    10201 cggctcaact gattgcacgt ttgctcgatt caatgggtaa agccgaaggt attttaggca
    10261 ctattgctgg cgatgatacg atttttataa cgccggtacg caatgtgcaa attgaaaaat
    10321 tggtgaagaa tgtgacacag ttatttgaaa catcattata gaataagtaa aggaggactt
    10381 aatgttctcc ttatttttta ggagattatt atgcacattt ttataacagg tggtacgggt
    10441 tttattgggc gtgaattaat ccagcaatta aaatctgaaa atcaccactt tacacttctt
    10501 actagagata cagaagcgaa aagtgatgat ttacagatta attattgcta ttcacttgat
    10561 gaacttgttg atttaaatga ttttgatgca gtaattaacc ttgccggtga gccgattttt
    10621 gataaacgtt ggacagacga acaaaaacag aaattgattg aaagccgggt taacctaacg
    10681 aataagctca gtcaattaat gaatgcaagc tctaaccctc cgcatacttt tctctccggt
    10741 tcggcaatcg ggatttatgg cgatttgcct ccttctgcaa atttttatac agaatcgacc
    10801 gcttgttatc aagattgctt taccaaccgg ctttgccaac agtgggaagc cgctgcacta
    10861 aaagccgcaa gtgataaaac ccgagtgtgt ttaattcgta ccggcacggt attttcgccc
    10921 gaaggcggag cattaaagaa aattttaccg ctttatcgat gcggattagg cggaaaactt
    10981 ggttcaggca aacaatattg ggcgtggatc agccgccggg atcacgtaca agcggtcatt
    11041 tttctgctca aaaatgcaaa ttgttcgggt gcttataatt ttgttgcacc aaatccggtg
    11101 agaaatgaag aatttaatcg tattttagcg aatgctgtta atcgcccggc attctttact
    11161 gtgcctgaat ttgccttgaa attaggctta ggcgaacgag cttgtttagt cttagataat
    11221 caacagctca ttccggaaaa actactcgca gccagttttg tattccaaga tccgatttta
    11281 accgagaacg ttttcttaaa cccataattt aggctataat gctgtataaa agcacagatt
    11341 aaaaggtgat tatggatttc tcattattat tagacggttt aaatgacaag cagagggagg
    11401 cggttgctgc cccgattggt aattatttag tgttagccgg ggcgggttcg ggcaaaactc
    11461 gggttttaac ccatcgcatt gcgtggctga ttggggtgga aaatgtgccg gaatccaata
    11521 ttctggctgt tacctttacc aataaagcgg cggcggaaat gcgacatcgg attgagtaca
    11581 ctctttccca atcgggcgat aatcgattgt tcggaatgtg ggttggtact ttccacagca
    11641 ttgccaaccg cttacttcgt tcccactatt tagatgcgga tttaccgcaa gatttccaaa
    11701 ttatggatac cgaagatcag caacgtttac tcaaacgttt actgaaactg cataatattg
    11761 atgagaaaca ttttccgcca aaacacgttg cgtggtatat caacgcccaa aaagataaag
    11821 gcaaacgggc aaaagatatt gatcactata acgatccgaa tgaaaaaaaa ttggtggaga
    11881 tttaccaaat ctatcaagat gcctgcgacc gagccggttt gttagatttt gccgagctgt
    11941 tgattcgggc ttatgaacta ttcaaaacca aatcaaccat tttgcaacgt tatcaacaac
    12001 gttttcagca aattttgatt gatgagtttc aagacaccaa caatattcag tacgacttaa
    12061 ttcgtttgct tgccggcgat gtcggcaaag tgatgattgt cggcgatgat gatcagtcta
    12121 tttatggttg gcgtggggca caggttgaga atatccaacg tttcttaaat gattatgaca
    12181 acgctcaaac cattcgctta gagcaaaatt accgctctac cggcaacatt ttaagctgtg
    12241 ccaatgaatt aattgccaat aacgataacc gcttaggcaa aaatttatgg acagatagcg
    12301 gtgatggcga gcctgtggat atttactgtg ccttcaacga acttgatgaa gctcgctttg
    12361 ttgcctcgca aatcaagcaa tggaaagaag acgagggtga tttggcggaa tgtgcggtgt
    12421 tgtatcgcag caacagccaa tctcgtgtga ttgaggaagc tttaattcaa gcgaatattc
    12481 cttatcgcat ttacggtgga atgcgattct ttgaacgcca agaaattaaa gatgctctgg
    12541 cttatttacg cttaatcgcc aatcgtcaag acgatgcggc gtttgaacgg gtgatcaaca
    12601 cgccaaccag aggcattggc gatcgtacct tagacacact ccgtcaaatt acccgtggtc
    12661 gccaaattac gctgtggcaa gcaattcaag tggcggtgca agaagaacaa ctttcagggc
    12721 gtgccacttc ttccttattg cgttttgtag agctgattaa tgctcttgat caagaaaccg
    12781 agcagatgtc gcttgccgaa caaaccgatt ttgtgattaa aaaatcgggt ttatatgaaa
    12841 tgtataagca agaaaaaggc gaaaagggcg aagttcggat tgaaaactta gaagagttag
    12901 ttaccgcaac taagcagttt gtgaaacctg acgaagcgga agagctgacc gatcttaccg
    12961 catttttaac ccacgcctca cttgaggctg gagaagccca agcctcgcaa cacgaagatt
    13021 atgtcgaact gatgacgcta cattccgcca aaggattgga gtttccccgt gtgtttattg
    13081 tgggcgtgga agaagggatt ttcccaagcg gaatgagctt tgatgaaggc aggttgcaag
    13141 aagaacgccg tttggcttat gtcggcataa cccgagctaa gaaaaaactg acgattagct
    13201 atgccgaatc aagacggctt tatggtaaag aagaacgcca cgttccgtct cgctttattg
    13261 ctgaattacc tgaagagcat attcgagaag tgagattgag aggctcaatt aaccgagcgg
    13321 caagttttaa ccaagctaat tcatttgcaa aaaatccgac aaaaacgacc gcttcctttt
    13381 taaacgatga tacttggaaa atggggcaga aagtgaacca cgctaaattt ggacaaggca
    13441 cgattattaa tgtggagggc tcaggtgaag ccacccgttt gcaagttgcc tttgtcggca
    13501 acggcattaa atggctgatt gccaaaatgg caaatttaga gaggatttag gcgaataaaa
    13561 agttttttgc aggaatatag atggcgagat atattgcccc ttaaccttaa actaagggta
    13621 aataaaatta aataatgcaa gctagatata atgtaaagta tcagaaataa gatattaatt
    13681 taagataaag aatagatggc tggggtacta ggattcgaac ctagggatgc cgagatcaaa
    13741 acccggtgcc ttaccgcttg gcgatacccc aaaagaaaaa actaacttac gttagcttat
    13801 atagatttga atgtttgaaa ttaagtggct ggggtactag gattcgaacc tagggatgcc
    13861 gagatcaaaa cccggtgcct taccgcttgg cgatacccca acactaattt ttgttaatcg
    13921 ataaatggtg cgggacgagg gacttgaacc cacacacctc gcggcgccag aacctaaatc
    13981 tggtgcgtct accaatttcg ccagtcccgc aatggtggct acagcgagat tcgaacttgc
    14041 gaccccaaca ttatgagtgt tgtgctctaa ccagctgagc tatgtagcca tttgcgttca
    14101 ccatatcggt caacggagtg cattatgctg attttagggc tttccgtcaa atactttttt
    14161 taaaaaattt gaatttttga ttcgcttgta tagttattcg tcgttttggc ttaaatctag
    14221 cattttttgt ttataaaata atagcttata ttctgctatt tcaccgtttg catttctaat
    14281 tttaattgag ccagtacagg ctcaacatcg gggaattgcc cttcccacaa agaaaacgca
    14341 aacgctgcct gtccaaccaa cattcctaat ccgtcttggc acgctgttgc tccacaacgc
    14401 cttgcataat tgagaaaagg agtttgcata ttgggggcat attgcatatc gtaaatattc
    14461 gccgatgcta taacagattc aggcaattca acatatttgc cttgcagacc gagagaggtg
    14521 gcattgacga ttaaattaaa cagttggttt gccaaatcgc ttaaacaagc ggtctgaatt
    14581 ttgccaaatt ttgcaaattt ttcggctaaa acgaccgctt tctcctgcgt gcggttatat
    14641 agagtaatct ctaaccccgc tttaagtaac ggaagtaata cgcctttggt tgctccacct
    14701 gctccaagaa ttaaggtttt atgccctttt tgtaaccaac ctagccgttc caaatcagaa
    14761 actaaaccgg ccccgtcggt attatcggca tataaccgtc catcttctaa gcgtttaagc
    14821 gtattacacg cctgtgccaa caaagcggtt tcgctaaatt catctgccaa tttaaaggct
    14881 ctttctttaa aaggggcggt gatgttgcaa ccttttgctc cagaagcgaa aaaatcggct
    14941 aattgttgct caaaagccgt ttcatctcct aagttagcga tatattcaat gttgcgttgg
    15001 gtttgtaaag caaagagttg atgaatacgg ggggatttac tttgagcaat cgggttgccc
    15061 cacacggcat atcggttcat ttcggcttat ccttgtctaa aaatctgttg gctgaaaata
    15121 tctcgaattt cggaagggtt ttctctacct agcgtgtctg cgtgtaatac aggaaaatct
    15181 tgaccaaact gatcgatgac ttcttttgca ttgcggcaag gtggttctcc gctcaaattg
    15241 gcactggtag aggtcaacgc aacgccgctt gcctcacaca actgaaccac cgccggcaca
    15301 ttacataaac gtacggcaat agaatcaaat ttgccggtga ggtaacttgg cacagtattt
    15361 ttaaccggta cgatccaagt aattgcctgc tcgccaatat ggcttaggcg ttgccaagcc
    15421 tgttccgtca ttcgagcttc atcaatatag ggaagaagat actttaaatc agaagccagt
    15481 aaaattagcc ctttttcctc agggcgttgt tttaatacga gtaatttcct taccgctttt
    15541 tcactttgtg gattgcagcc taagccaaaa accgcttcgg ttgggtaagc tacaacttct
    15601 tcttgcttta attgattaac tatttctaaa aaattattca ttcggtttta attctgtttg
    15661 gtgctggcaa cttttgttag cacaaatggt gaattgcttg cccttaattt ttttgattgt
    15721 agcgagttca caaccacatt tttcacacaa gtggttaatc ggtcgagttg ctaatgtaaa
    15781 tttgcaatta gggaagccta tacaaccata aaatattttg cctgaacgcc cttttctagc
    15841 aatcagttta tgttgcttgc attcagggca atcaaactct tcttcaacct cagcttcttc
    15901 gtgtacgatg aagtggcatt cagggtaatg gctacagccg ataaaaatac cataattgcc
    15961 ttgttttaat tggaggtttc cgccacattc agggcaaagt tcatctaatt gcttaataat
    16021 atgaccggct tgatgtaatg gcttaatata atcacattca gggtaagctg aacaacccaa
    16081 aaataaccct tttttgccct gcttaatttg caacggcgag ccacattgtg ggcaagtttc
    16141 ggtctgtttg gttgatttga aaagtaccat attgatttgc taaataagcg taaattcaat
    16201 cacaaaaaac gcattgtcat tcggataaat ctcccgaata acttgtttca attcggctaa
    16261 gctcatattt tcttgtttag cgtgttgctc gtttaattca tcaaacacta taggtgttac
    16321 cgatagcact ttaatattgg cgaaaaatct atcggtttca ttagtaaaaa ccgctaattc
    16381 ttggtttggt tgaaaataag attctgactt gtctcgaatc gtaattgttt ttttaccgct
    16441 taaaatatcg gcttcaaatc ggctgaaaaa ggtaattttc tctctattca tattcatcgc
    16501 tactgtaatc cagagccatt aaatgactaa aatgatcgtt ataaaaacca cggctaacca
    16561 taaatcgcca cattttctgc tttgctttca gatcccatac aagcggtcgt tttttctcaa
    16621 aaatttgctc agctaattca aaccaatcca cttcggcgtt ttctaattct tggttaatta
    16681 accaatcttt taccccttta agttttagct cttgctttaa acggcgaggc ccaatgcctt
    16741 gcattgaacg ataacggata aaagtggtac aaaagcgttc atcactttgc cactgattcg
    16801 cttgagcttt ttcaatcgct gcatcaatct ccgtgagttc atactctttt tgctttagtt
    16861 tttgccgaag ctcttgctcg ctgtaatcac gctttgaaag taaatatagc agataattaa
    16921 ccgctgtgta tttatttgtt ttagtagtag atgttgtcat ttatcggtta taaataaaaa
    16981 tgcgattttc acgttgagcg aaaaccgcat aaatttaatt agagttcttc ttcactaaat
    17041 tcatcatctg cattgtctgc attttcagaa tctggagtag ccgtaaaagt tgggtttgaa
    17101 atgagtagat ttcgaatatc ttgctcaatt ttactcgcca cttcagggtg ttcttttaac
    17161 catttgatgg cattattttt accctgaccg attttctccc cttcataaga gaaccaagcc
    17221 cctgctttat taatgaagcc gtgagattcc gctaaaatga gtaattcatt catacgggca
    17281 atgccctcac cgtacataat atcaaattgg acttcacgga acggtggagc aaccttgttt
    17341 ttcacaattt tcacttttgt ttcgctaccg ataacctcat caccgtcttt tactacgcct
    17401 gaacgacgaa tatctaaacg tactgaagca tagaatttaa gggcattacc accagttgtg
    17461 gtttcagggt taccgaacat tactccaatt ttcatacgaa tttggttaat gaaaatcacc
    17521 aagcagttgg tcgctttgat atttgcggtt aatttacgca atgcttgtga cattaaacga
    17581 gcttgtaagc ccatatggga atcacccata tcgccttcaa tctctgcttt tggtgtaagt
    17641 gccgcaacag agtccacaat aattacatca accgcaccgg aacgaactaa cgcatcacaa
    17701 atttctaatg cttgttcgcc attatccggt tgtgaaatta ataatccatc tgtatctacc
    17761 cctaacttac gggcgtaaat aggatcaagt gcgtgttcgg catcaataaa ggcacaagtt
    17821 ttaccatttt tttgggcttg agccaccaca gataaagtta atgtagtttt acctgaagac
    17881 tccggtccat aaatttccac aatacgaccc attggtaaac caccaatgcc taatgctgag
    17941 tctaaaccaa gagaaccggt tgaaactgct tcaatatcaa gggcttgagt atcgcctaac
    18001 ttcataattg agcctttgcc aaattgcttt tcaatttgag ctaatgcggc tgccaacgct
    18061 ttttcttttt gttccggatc aatctgttta accggagtgt tcttttgact ttttttctct
    18121 gccatattct atcctttgta aacttttatc taaaagcaac cgcttgttgc tgcaaaataa
    18181 ctgtatatta ttatagtatt tacttatcca gttgcaagca tttttttagg attttcgatc
    18241 aagatcacaa aaataaattt gcgagcaagg caatgccctt ggttcctcaa agggattaag
    18301 agcattaatc aacataattt tttcatattg atgcaaatct tgcttggcta ttttggctaa
    18361 aacgactttc ccttcatcaa gcaatttact aagctgagtg cctttgagca aataatgttg
    18421 cgggctatac cagattcctt gttttaggaa cagtaaattg ccgatggagc aatcactgac
    18481 gtagccatta ttgagaataa tcacctcttc attttgtaaa atagggagca aatcaaaccg
    18541 cttgcggtcg ctaaatttga ggtgataatc ccaattttcc acctcaacca agcgaaaacg
    18601 ttctactttt ttgggcgaat agataaaaaa tttcagctca aattctgttg cgttgtaatc
    18661 tattcggcaa cgtactttgc cccgttgaga agcctctggc acaactaaaa tatcagcaag
    18721 gctaaaacga ggttcacagc caaaatattc ttgtgttgct tgctcaaaac gttgttgatg
    18781 atactgtaag ttctgaactt tgccgtcaat aatcgaaagg gtttcaaata gcggaaatcg
    18841 gcacatagac tttctctaac agttcattat attcatcagt taacacactt tgtgaggtaa
    18901 tgccaccacc actatgaaac acaagcccgt ttgaggtttg agcaatgtaa cgaattgcca
    18961 ccgcactttc taagctctcg ccatcaaaat aaccaaaaat accggtgtag taaccccgtt
    19021 ttcctaattc tgcttgctga ataatcgaaa ccgttttctc tttcggtgca ccgctaatgg
    19081 agccggcagg tagtaatttt gccaatatcg aaccgatatt ttcttgccaa ttttcaccta
    19141 attcgccaca gatctcggaa cttgtctgca aaattgcccc tcgatgggtt tctacttttt
    19201 ccacataacg gtatttagta acttcaatat ttttcgccac caacgccaag tcatttcgga
    19261 tcaaatccac aatggtatta tgttcggtaa actctttttc cgaagccatt aatttctgct
    19321 cggcattctc ctcatttgca ttaattgtgc ctttcatcgg gtaggaaaaa atgcggttat
    19381 tttcaattcg cacaaagcat tcaggggaaa aacagacgaa cctgccttgc aaataacatt
    19441 tatattttgc tttgctggcg gcaaaaattt ccgccaatga ataattggtt tcaatggcag
    19501 tcgggtagct caaattcagc aagtagctat tgccggcttg aatttccttt ttcactaatt
    19561 caaacccggt ttggtattcg ctaaacggaa tcggatttgc cgataattca aaagctttag
    19621 tcggtttttc aaccggcttt tcgagattcg atttgcaaaa aaattcaaaa aaaagaccgc
    19681 ttgcgtgagc ctgttctagc ggaaaaataa gtggcttttg ctgctcaaaa tcaattaaaa
    19741 agaaaaaagg gcgttttgct tgcccgtagt gattggcttg gtgaataaaa tcttgcatag
    19801 aaaacacaat ggctgaaatt ggctgaatca tcaaaagctc tgttataatg caaagcaata
    19861 gatttgaaaa gcatttttta aaaaaggttt taccgatgaa caacaccatt cattttcaag
    19921 aaattgggat ctacatcgcc cttgccgttt gtgtgctggt aggcgtttat ctctttttct
    19981 tgaaatcccg ttatcagcga aatgcctacg aattaagcca agatcttggc aaaatcaccg
    20041 aaaattttga acaaattcag caaaaatatg aggtgttggc tcaagaaaaa aatcagcttg
    20101 agcagtgggc aattcaacaa cagaccaaat atgaagcagt gagtgaacgt ttaaacgagc
    20161 gagatgggca attgcaacga ttccaacaaa aaatcgaact tgccgaacag caagaaaacc
    20221 agctggaacg ctatattaat gagctaaaag aacgtgtggg ttcttcccaa gcaaaggcag
    20281 aaagtttgga agaacagcta caatttagcc aaagttcact ctcaactaaa gagcgggaaa
    20341 atcaagcgtt atttagccgt ttaaacgagg tacaaaacga gctgaccgaa ctgcgtacta
    20401 cgctttcgga aaaacaggca aattttgaag cacaacaacg caattttatt gaggtaaaac
    20461 aacagcttaa tgtggaattt caacatttgg cacaacaaat tcttgacgaa aaaagcaagc
    20521 gttttagcga aaccaatcaa agctcgttag aagctctact gaagccgttc aaagagcaaa
    20581 ttgaaggctt tcaaaaacgg gtgaatgagg tacatagtga gtcgctcaaa ggctcggcaa
    20641 atttagaggc tgaaattaag cgggtgctac aaattggcgt ttcaatgtcg gaagaggcac
    20701 aaaatctggc aacggcatta aaaggcaata ataaaattgc cggcaactgg ggtgaagtac
    20761 agcttgaaag tgccttacaa tctgccggtt tactcgcagg cgagcattac caagcccaag
    20821 agagttttcg tgatgaagag gggcgacgtt ttgcccccga ttttgtggtg catttgccgg
    20881 atcaaaaaca tctgattatt gacagtaaag tctcgttagt agcgtacgat caagcggtca
    20941 gatcagaaga aaattttgca atttctcaag ccttagacga acattgccgt tcgctacgca
    21001 atcatattga gggattgtct aagaagaact acagtgcatt attaggcatt aaaagcccgg
    21061 attttgtact gatgtttatt ccgatagaac cggcttatat tgaagcgatg aagcacgatc
    21121 cgcaactgtt taactttggc tatgaacgca atgtgatttt ggtttctcac accaccttaa
    21181 tgccgatttt acgcacggtc gccaatttat ggcggattga acgaggcaac agcgaggctc
    21241 gtgaaatcag cgaaaaagcc ggcgaaattt acaaccaagt gtgtgcggtt gccgatcggt
    21301 tagctaaatt aggtaacacg ctaaataccg caaataatca atacaatcaa accgttacct
    21361 cgttagtggg acggcaaggc ttactgggca aagtggaacg attccaacat ctttcaagca
    21421 aagcaagcca aattgtgccg caggttgagc tgattgaaaa tgaggtggat actattaaat
    21481 tggaaatggt atcgagagaa ttattggaag aatagtcaaa tctctttatt ccctacaaaa
    21541 atatggggtg atttgaaata ctaaagaaaa taataaaatg ctaaaaaaac tattaatcat
    21601 cgacaaccac gactctttta ccttcaactt ggtggattta ctccgtaagc ttgatgtgcc
    21661 ggcgaaagtg gtgctggttg aagagcttga cctcaatgag gtggagcatt ttagccatat
    21721 tttgatttcg cccggccctg atgtgccgag agcctatcta caaacctttg cgatgttgga
    21781 gcgttatcat caaaccaaat caattctagg cgtttgcttg gggcatcaaa cgatttgcga
    21841 gttttttggc ggcaagttgt ataacctaaa cgatgttaga cacgggcagc aacgcacgct
    21901 cactcaaatt gagcctaacc ccatatttaa aggtttgcct aagcagttga aagttgggtt
    21961 gtatcactct tggggcattt tgcaaaattc gttgcaaaat acaccgcttg taccaaccgc
    22021 agtttgcgat caaaatgtgt taatggcgtt taaacaccaa catttgccga tttttggcgt
    22081 gcagtttcac ccggaatctt ttatcactga atttggtcaa caaatgttgg caaattggct
    22141 tgagattaca caagattttt agcgtgaaat ccgctataat ccgtcagtta tttacccttt
    22201 taaaatctgt aggggcgaaa aatttttcgc cttacttaat atgcaagaga taatttaaat
    22261 gaaagattcg attatcaata aattagaaag tttaagtgaa cgccacgaag aactacaggc
    22321 gttacttggc gatgcctcaa ttatttccga tcaagacaaa ttccgtgctt attccaaaga
    22381 atattcgcaa ttagaagaag tggttggcac ctttaaccgc tggaaaaagc tgaacaatga
    22441 tattgaagag gctcaattat tactcgatga tcctgatatg aaggatatgg cagcggaaga
    22501 aattgcggaa aataaagccg aaatcgagaa tcttgagcaa catttacaaa ttttattatt
    22561 gccgaaagat ccgaatgatg aatacaacgc cttccttgaa atccgtgccg gaacgggcgg
    22621 tgacgaggct ggtatttttg ccggtgattt ataccgtatg tacagccgtt attgcgaaag
    22681 taaacgctgg cgaattgaag aaatgtcggc aaatgagagt gaacaaggcg gatataaaga
    22741 gatcatcgtt aaaatttcag gcgaaggcgt ttacggtcag cttaaatttg aatcgggcgg
    22801 acaccgtgta caacgtgtac cgaaaaccga atcacaaggg cgtattcaca cctctgcttg
    22861 cacggtagcg gtaatgccgg agttgccgga aagcgagatg ccggaaatca atccatctga
    22921 tctgcgaatt gacacctacc gctcatcggg tgcaggcggt cagcacgtta ataccaccga
    22981 ctctgcagtg cggattactc atatcccgac cgggattgtg gtcgagtgtc aagatgagcg
    23041 ttcacagcac aaaaataaag ccaaagcgct ggcagtgtta gcctctcgga ttgtacaagt
    23101 tgagcaagaa cgccaagccg cagagcaagc ggatacccgc cgtaacttac ttggctcagg
    23161 cgaccgttcg gataaaatcc gcacctacaa ctacccgcaa gggcgtgtga ctgatcaccg
    23221 tattaacctc acggtttatc gtttagacga agtgatgaac ggcaaaattg atgaattgat
    23281 acaaccgatt atcactgaat atcaagcgga tcagttagcg gcgttgtcgg agcaaaatta
    23341 attttattgt aagggtacac tgtgtgtgcc tgccaaatat aaatttgcaa aattttgact
    23401 atttttgatc gcttgtagtt tctaaaaagt ttaaaaattt gaggaaaaca aaatgagaaa
    23461 gttaggttta ttagcagtat ttactttagt attatcagcc tgttcaaatt caacaaaaat
    23521 tgaacaaacc attctttcca agcaggatat cgtaggctct tgggtatgcg ctattaaata
    23581 cgatgatcta aaagtaagca ctttagattt ttctgaattt aaagctaatg gcgaaatgac
    23641 gaatttcggt gatatttctg atgagaattt tgagcccatt aaatttactt atgtaacagg
    23701 agataaaggg aagtgggact taaaaggaaa tcgattagtg attgattatg atctttctga
    23761 acgtaaagta aaaagaacaa caccaaaaaa atttttagag ttcttgaaac aggaagagcg
    23821 taaagcctca aaaacagcta aaaaactcct taaatatgaa caagggattt tcaatattct
    23881 gagtgatcag aaaaatactg aaaacagtaa aataatatta gatattttga agtttggtca
    23941 ttcaagaatg gcgattcagc aaaatatggg agacaaagtt tattcaggtg gttgtgtaac
    24001 agctgataaa gcagaagagt atttagatat cctaaaaaat agaaattcta aataatggat
    24061 taccgagagt ggctcacttt tgctgagcaa aaattacttg aaaatagcgg ggcagatcca
    24121 tacctaaacg caaaatttga tgcaaatttg ctattgcaag cagtcacaaa acgttcaaaa
    24181 tcggccattt ttgctttttc ggaaacccta ctttctgaaa gcgagcttgc agaattagcg
    24241 gaaaaattag ctcggcgtgc caaaggtgaa ccaatggcat atattttagg ctatcgggaa
    24301 ttttggtcgt tgccgttaaa ggtttcaccg gcaacgctga ttcctcgccc tgatacggaa
    24361 cgactggttg agttggcgtt ggaatatgct aacaagcggt taggaaatca gaaaatgttg
    24421 caaattctgg atctcggtac cggcacgggg gcgattgcgt tggcactggc gagtgaatta
    24481 ggcgagaaag cccaaattat cggggtggat ttccaaaatg aggcggtgca actggcagaa
    24541 gaaaatcgcc gaaacttagg cttaaatcaa gttacatttt tacaaagcga ttggttcagt
    24601 tcgcttgcaa atcaacaatt tgatttaatt gtctcaaatc cgccttatat tgatgaatcg
    24661 gacgaaaatt tgacggtggg agacgtacgt tttgaacctt taaccgcatt agttgccgat
    24721 aataaggggt tgagcgattt gcaaaaaatc atcgaaaatg caccgcttca cttaaaaccg
    24781 caaggggcgt taatgctgga acacggctgg aaacaaggta acttggttcg agatttattt
    24841 gatttacact tgtgggaaca ggtagaaact gctcaagatt atgccggcaa tgaccgagta
    24901 accaaagcga ttttaaaata aggcagaaaa tggacgatat tattgaagaa ttgttaaaag
    24961 agttggaaga tccgaaagta accatcagcg aaaccgagct gaaaaagttt ctttatcgtg
    25021 aaattttacg attaactacg ctcattgata agaatgtaag tgagcaacaa gtgtttggtc
    25081 agatgtctgc gttggtgaaa aaagctcgct ataacgtgaa tggcgaaggc gatgaagaga
    25141 gaattaacca attacttcat ttagtctatc gtgaatgggg ctttcattgc tactatgaag
    25201 actatttcca caccgaaaat ttgttgctaa atcaagtaat tcgcaaacgg cgtggaatgc
    25261 cggtttcact tggggcggtg gcgctttatt tatccgccgt attggatttg ccgctttacc
    25321 cggttaattt tccgactcag cttgtgttac gggctgaaat acaccagccg aacggggcaa
    25381 ccaaagtgcg ttttatcaac ccgtggaacg gtgaatttct caccatcgaa atgctgaata
    25441 aatggctgga aggagaaatg ggctttggcg tagaagttac ccctgatttg ttaagacgag
    25501 cagaaccaca agagttgctg gaaagggtcg aaactgtgtt taaaatggca cttacccgtg
    25561 aaggcaaata tgaagaaacc ttacgcttga ttgaataccg cttggcattt agcccggaag
    25621 atccgtacga aatcagagac cgaggaatgg tactggcaag tatggattgc taccaagcag
    25681 cattagaaga tattaattat ttcattgacc aatgccctga agatccttct gccgaaattt
    25741 taaaaatgga agtgaaagga ctgcaacaaa aaagccaaga aagtgtggtg cattaagctg
    25801 agaagtagcc ccaaaggggc tgagctatga ttaacctagt acggctctgc cgtgctaggc
    25861 agaaaataag aaacaaggac tccctatgac agataaaatt gtaaaagttg gcaatattga
    25921 agtggctaat cacaaaccgt ttacgctttt tggtggtatg aatgtgctgg aaagccgtga
    25981 tatggcactt agagtatgtg agcaatatgt tgaagtcacg caaaaactaa atgtgcctta
    26041 tgtgttcaaa gcctcatttg acaaagcaaa ccgttcttca atccactctt accgtggacc
    26101 gggaatggaa gaaggcttaa agattttcca agaattaaaa caaacctttg gcgtgaacat
    26161 cattaccgat gttcacgaaa tttaccaatg taaacctgtg gcagaagtag ttgatgtgat
    26221 tcaactacca gcatttttgg ctcgccaaac cgatttagtg gaagcaatgg ctcgtaccgg
    26281 tgcggttatt aacgttaaaa agccgcaatt tttaagcccg ggtcaaatgg ggaatatcgt
    26341 agaaaagatt gctgaatgcg gcaacgatca agtgattttg tgcgaccgtg gcactaactt
    26401 tggctacgat aatctagttg tggatatgct tggtttcggc attatgaaaa aagtgtcgaa
    26461 aggttgcccg gtgatttttg atgtgactca ctcattacaa tgccgtgatc cattcggtgc
    26521 ggcttccggt ggtcgcagag atcaagtaac cgaactggca cgctccggta tggcgattgg
    26581 tttagccggc ttgttcttag aagctcaccc ggatccaaac tcggcgaaat gtgatggtcc
    26641 atctgcatta ccgctgtcaa aattagaagc ctttgtttca cagatgaaag cgattgatga
    26701 gttggttaag agctttgaag aaattgatac ttccaaataa actttctccg cattaaatcc
    26761 tttcctactt tctttgcttc gccaaagaaa gtaggcaaag aaaggcgacc ctacttcgct
    26821 acttttcttc gcttagtgtg aatgggctta acgaaaaatt tacattcgct tcgctcagag
    26881 taaattttcc tacccattaa aacacgctca ggtagctcag aagggagtta gaaaggtaaa
    26941 agacagaata ggagaaaaaa atgctaaata ttgtttttct tgaccgtacc ggtattcctg
    27001 ccactcacga tattcctcgt ccgaattttc cacatacttg gacggaatat gccaccacct
    27061 ctgccgatca aacttttgag cgggtaaaag aagcggatat tatcattacc agcaaagtgt
    27121 tactccagcg tgacttgctg gctaaattac caaagctgaa attaattgcg atcacagcaa
    27181 ccggcacgaa caatgtggac ttagcggcag caaaagagtt agggattgca gtgaaaaatg
    27241 taaccggcta ttccagcgtt accgtgccgg aacacgtcat tggtatgatt tactccctga
    27301 aacatcgttt agccgattat caccgtgatt taattacgtc tgaccgctgg gcaacttgcg
    27361 gccaattttg ctatgtagat tacccgatcc acgatgtgcg aggctcaacc ttaggcattt
    27421 tcggtaaagg gaacatcggg gctgaagtgg cacggctggc acaagcggtc ggaatgcagg
    27481 ttatttttgc agaacatcaa ggcgcaactg aaattcgaga aggctacacc gcctttgaag
    27541 aggtattcaa acaagcagat attatttcac tacactgccc tttaaccgag cagactcaaa
    27601 acctgattaa tgcagaaacc ttagccttaa tgaagccgac cgcttatatt atcaatacgg
    27661 gtcgaggccc actgattgat gaacaggcat tgcttgaggc attagagcaa ggcaagattg
    27721 ccggcgctgc gttagatgtt ttagtgaaag agccgcctga aaaagacaac cctttaatgt
    27781 tagccgcaaa acgtttaccg aatttactga ttaccccaca cgttgcgtgg gcaagcgatt
    27841 ctgcggttac aacactagtg aataaagtga cacaaaatat ggaagagttt gtagcaacgg
    27901 gtatgtagct tgaagtaggg gctggtcctg tgccagccct aatattaata gaatgagagg
    27961 gcgggtataa aacccgcccc taccaatatc attttagaaa aacagatgaa tttaacccct
    28021 ttttttattg cttttcgcta ttttagatcg aaaagtgctg accgtttcgg gcggttagtc
    28081 accaatcttg ccagccttgg cattgtgctg ggcgtaatgg cattggtgat tgtgctgtcg
    28141 gttatgaacg gtttggaaaa tatgcagaaa accaatctgc tatccacctt gccgcacgct
    28201 atcatcaccc ctcaagaggg gcattttgcc aaaggtgaaa aactggctct acctgaattt
    28261 gccaagcaaa gtgtggtgat taaccgtgcg aatgtgatta tccaaagttc acaaggcatt
    28321 aacgccggtc agttaatcgg cgtggaaaat gccacagacg atccgttgtt agtggcggaa
    28381 aatatccccg cattattgcc tagcggtgaa tttaaggtag taattggctc tcgtttagcc
    28441 aataaattga acgttacggt aggcgataag ctccgcttaa tgatcactga aaacagccaa
    28501 tatacgccac ttgggcgagt gccggtacag cgtttgtttg aaatttcggg cgtttatcaa
    28561 tctaatcgtg aagcgagcga atttaccctc tttgctaatt tagctgatgt cgggcgtttg
    28621 cttcgcatca gcgaagatca agtacaaggc gtgcgtttat atttgcaaga tcctttccaa
    28681 gttaccgaac ttagccaata tttcgatgca gaacgctacc aaatcagtga ttggcgtgag
    28741 caaaaaggcg aatttttcca agcggtgaaa atggaaaaaa atatgatggg cttactcatc
    28801 agcctgatta ttgtggttgc aatttccaac attgtgactt cactcagttt gatggtggta
    28861 gacaaacagg gcgaaattgc aattttacaa acccaaggct taaccaaaaa acaagtgatg
    28921 cagatttttg tgttccaagg ggcgattgtc ggagtaattg gctcgattat tggtggtttg
    28981 ctcggcgtat tgatcgccta caatttagat cagattatcc tgctattaaa cccgagcatc
    29041 catctgccta ccctaatttc aggcacgcaa attgcggtca ttgtaggggt gtcgatgttg
    29101 ctttcactcc tttgcacgct ttacccggca taccgagcct caaaaattga gccggcacag
    29161 gcattgagat atgaataata agaacggaaa taaaatgaca gaattacttc gctgtgaaca
    29221 tatcagcaaa ttttacgatg agggcgaaca gaaagttcag gtgctaaaag aggtgtcatt
    29281 ttcgatgaac gaaggtgagc tggtagcgat tgtcggtagt tcaggttccg gcaaaagcac
    29341 cctattgcac acattaggcg ggctagatca gccaagcacc ggtgaagtct ggattcgagg
    29401 gcaatctctg caacagctca gttcagacaa attagcgtta ttgcgtaacc aaaacttagg
    29461 ctttgtgtac caatttcatc acttaatggc agactttacc gcccttgaaa atgtgatgat
    29521 gccgatgctg atcggcaaac agaacaaaac tgaagctgcc aatcgtgccg agaaaatgct
    29581 acaagcggtc ggattagctc atcgaatgac acatcgccca tcagcacttt caggcggcga
    29641 acgccaacgg gtggcgattg cccgagcgtt ggttaataat ccggctctgg tacttgccga
    29701 tgaaccgacc ggtaatttag accaaaaaac cactgaaagc atttttgagc tgattcaaca
    29761 gctcaaccaa gagcagaaca ttgcgttttt attagtaacg catgatttaa atctagcgaa
    29821 taaactttca cgtagtcttg taatgcgaga tggcgtgtta agcgagggga gcttatgagt
    29881 acgccgtttt tcattagctg gcgttaccaa cggggcaaac agaaaaacaa actggtttca
    29941 ctgattgctc tcttttccaa catcggtatt gcccttgggg ttgcggtatt aattatcggg
    30001 ctaagtgcaa tgaacggctt tgagcgagag ctgaataatc gtgtgctttc ggttgtgcca
    30061 cacgctgaac tggtttcgtt tcaaggcaat cagcctcaac caatttttga tagcaaaaaa
    30121 ttagaggcat tgataaaaaa atcgcaaaat gtgaccgcta gttcgccttt tgtcagcttt
    30181 accgccttaa ttgaaaatgg ggctcagctt aaaatcgccc aggtgcgagg ggttgatcct
    30241 aaaaagcaag atcaggccag ccaattaagc caatttattc cacccgagca atggcaagcg
    30301 ttcaatcagc aatttcaagc cgatgaagat ggcttgattt taggagccgg tattgccaaa
    30361 gcgttagaag ttgaggcagg agacgaagtt acgcttttaa ttcctcagcc aacagaagac
    30421 ggcaaattag cccagccctt gcgttttaac ctaaacgtaa ccggcatttt gcggctagat
    30481 gggcagctgg atcacagtta tgccttaatt ccactgaata aagctcaaga gctgatggaa
    30541 taccaagcga atgaagtgtc agggcttgaa atcagccttg ccaatccgtt tgatgtacaa
    30601 cagcttgaaa tgccacaact taacggctat acccaaccgc tttatttgaa tacgtggatt
    30661 gataaattcg gttatatgta taacgatatt caactgattc gcaccgtgat gtacattgca
    30721 atggttctcg tgattggcgt tgcctgtttt aatattattt ccaccttgat tatggcagtg
    30781 aaagacaaac aaggcgatat cgcgatttta cggacactgg gggcaaataa cggttttatc
    30841 cgccgcattt tcttatggta tggcttaatt tcaggtatga aaggggcgtt attcgggctg
    30901 attttaggtg taattttatc gctaaattta accgctatta tcaaagcgat tgaagcattc
    30961 tttgaggtga aattgctttc agacggggtt tattttgttg atttcttacc aagtgaatta
    31021 cattggcaag atgtcgtcta tgtattggca gccacgatta tcctaagcct ttttgccagc
    31081 ttataccctg ccaaccgagc cgctaaatta gaaccggcaa aagtgttaag tgggcattaa
    31141 tcattttcct attctaataa aaaagcagct acccctcgat tatgtcagaa aggtagctgc
    31201 ttttatttat gttattactc ttcgcctaat aatttcaact cacgagcctt aatttggttt
    31261 tttagaattt caactaagtc tgaaacgagg taagtaccta agtctgcacc tttacgggta
    31321 cgcacagaca ctttaccttc ggcgatttct ttatcaccgc aaaccaacat ataaggcaca
    31381 cggcgtaagg tgtgctcacg cactttgaag ccgacttttt cgttgcgtaa gtctgctttc
    31441 acacgaatgc ctgcatcaga aagctgtttc accacttgtt gcacataatc tgcttggctg
    31501 tcggtaatgt tcattaccac tgcttgggtt ggggcaagcc aagttgggaa gaaaccggca
    31561 tattcttcgg tgataatccc gatgaaacgt tcgatcgagc ctaaaatcgc acggtgaatc
    31621 attaccggtg tgcggcgacc attatcttct gctacataag aggctgctaa acgacccggt
    31681 agagcgaagt caagttgcac tgtgccacat tgccattcac gtcctaagca atcacgcaat
    31741 gcaaactcaa ttttcggccc gtagaatgca ccttcgcctt cttgaatttc atattcaagt
    31801 ccgttgttgc gtaaggcgtt ggctaagcct ttttctgctc tgtcccacat tgcatcatca
    31861 ccgatacggt tttccggacg ggttgaaagt tttactgcaa tgttagtaaa accgaaggtg
    31921 ctgtaaatgt cgtacaccat tttgatacag ctgattactt ctgactcgat ttgttcttca
    31981 gtacagaaaa tatgggcatc atcttgggta aaaccacgca cacgcattaa gccgtgtaat
    32041 gaaccggacg gctcgttacg gtgacacgaa ccaaattccg ccatacggat cggtaaatca
    32101 cggtaagatt tcaagccttg attaaagatt tgaacgtgac ccgggcagtt catcggctta
    32161 attgcatatt cacggttttc cgattgggta gtgaacatta aatctgcgta gttttgccag
    32221 tgtccggttt tttcccaaag cacacgatcc atcataaacg gaccttttac ttcttggtaa
    32281 tcgtactctt tcaatttggt acgcacaaaa gtttcaagtt cacggaaaat cgtccagccg
    32341 tcattgtgcc agaacaccat acccggtgct tcttcttgca tatggtataa atctaacgct
    32401 ttaccgatac ggcggtggtc acgttttgcc gcttcttcca aacgttgtaa atattcggct
    32461 aattgttttt tatcagccca agctgtaccg taaatacgtt gtagcatttt atttttgctg
    32521 tcgccacgcc agtaagcacc cgctactttt tgtaatttga agtggtggca gaaacgcata
    32581 tttggtacgt gtggaccacg gcacatatca atatattctt cgtggtgata taacgcaggc
    32641 gttgccgtac gttcaatatt ttcatctaaa atcgccattt tgtatggctc accacgtttc
    32701 tcaaaggtat ctcgagcttc ttgccagctt accgggcgtt ttaccacgtc atagttggtt
    32761 tttgccagct ctaacatacg cttttcaaga gcgtctaaat cttcttgagt caaagagcgg
    32821 tctaaatcga tatcgtagta gaaaccgttt tcgatagtcg ggccgatagc cattttaacg
    32881 tctgggaata attgcttgat ggagtgacct aacaagtgag cggttgagtg gcgaatgatc
    32941 tctaaaccat cttcgtcttt ggcagtaatg atttcaagtg tgctgtcttg gctgatgata
    33001 tcagacgcat cacggcgttc accgtttaca cgaccggcaa tggtggcttt cgctaaacct
    33061 gccccgatag attgggcaac ttccattaca gaaactgggt tgtcaaatga acgttgtgaa
    33121 ccgtccggta aagtaataat tggcataata aatccttaca gtggtaatcc atacgagaga
    33181 ttacttgtgt tgtttgaatg aaaaaaccgc ctacaagcgg tcttttttga ttaaaatttt
    33241 gcaatttgct tattatttcc cttacaagtg gggaataaaa gcactgaaat tctagcataa
    33301 aacaaagaaa aattaaactt tgctgttttc agcggcccct tcatcaagct caatattttc
    33361 attttctgtt tgtggatctt gttcaagtat ttcaagctga gcctggtact cttgtaccgc
    33421 ttcttcatca aatggaataa gtgcgtcctc aacaacttca atttcttcaa gtgggcttgc
    33481 ggttgcaatt ggggtagcat attgcatctc tgctcgttcc gctttcggaa ttgatttggt
    33541 gcggaaagca caaataaata aactaagtga gaacagtcca aaaagtgcat aaaagccata
    33601 ttcagcggaa aggctcatcg caaaaccgat aagcggtgga gcaactaacg cccccacacc
    33661 atagcaaaac agtaaacccc tgcttacttc aaccgtgttc atattattcg gcaattcatc
    33721 gttggcacgg gcaatgctca aagcatataa gctaaacaga ctacagccaa agaaaaaggc
    33781 gataatatac tgtccccagg cggtcaaatt ttcttggaaa attgcaaata atccggcaag
    33841 tgcggttaaa gtggcaatca ctgaacaagc taaaatggcg ttacgtctgc caatacgatt
    33901 cgataatatc gccatcggca tttggctgat aaaaccaccg atcatcgaga taatcaaata
    33961 aagcgagatt tcacttaatg aaaagctaaa cttcagcaga aagactgacg ccattgtaaa
    34021 aaagccgttt accaacattc cgctaataaa gctggttgct aaggctaacg gtgaaatcgc
    34081 aaatagttta ggaatactaa tgcgctctcg agccggaata tcaggctgaa ccattttagt
    34141 taaagcaatt gggatcatcg acatcacaac caaaatggta gataagataa aaatgtttcg
    34201 gctactaagt tctaaactta ataaacttac gcctaaaata aaggcgagat agaagataat
    34261 gttataagtg gcaagcactt tgcctctggt atcgacattg gttttttcgc ttaaccaact
    34321 ttcgactaaa agcagcaagc tataatgaca gaatcccaaa ataattcgta acacgcccca
    34381 gatccacaaa gtatcaatca taatatgacc gagagcggcg atagcaaaaa cagcacagaa
    34441 aacgctaaat gaacggatat gcccaacact tgatacaatg cggtgagcaa aaatagtgct
    34501 taatgccgcc ccaataaaga aagcagcgtt aagtaaaccg atgatgatgt ccgttatccc
    34561 catttctgcc agtttgatcc ctgctgaatt tagaaataaa ccgtagccga aaaacagaaa
    34621 aaagatacct gaataaaggg agtaaaattt agtgctattc atcaatgctc cttcaaacat
    34681 tctgccagta ttaaatcatt gtaccgattt aatacctttt aggcaaaaaa gttattatta
    34741 gcgttgagaa aataactttt ggctattttg attaaaaaat caaatgaagg tgattttgaa
    34801 atgtagtaaa aaatagagca atcagaattg aaaaagggtt tgccgtaaca aagcctttag
    34861 aaagattaga gtttcgagaa gaatttgtag cttgggctaa ccaaaacact tgctaggagt
    34921 gctttaagca caccacctaa aataaatgga tataagcccg cttgtaatac tgtgccttct
    34981 ggtacaaaga aagttgagcc aagccgaaag caaaggtgat cacacttgca attaaggcga
    35041 caaataaact tttccaaaaa gagtttaata cgcctttatc gctataatag cctaagaaat
    35101 aggcggttgg aatatagcct aataagtatc cgcctgttgg accaaagaaa tgatggaaac
    35161 ctgaagcacc gccggcaaag acaggtaaac caatcgcccc ttcaaaaaga taaagtaata
    35221 ctgcagcagt tgcggcttta cgacccattg caaaaccaac cacagttaaa gcaagtgttt
    35281 gccctgtaat gggtacaggg tacatcggca cagaaatttg tgcaaaaata gcaagcagtg
    35341 ttgctccaat aaaagttttc aaccaaaatg tagttatact attggcttct agccaacgtt
    35401 tagtttgaat taattgcgta gtgaacataa tcctctcctt taatatggtt aaaatgttct
    35461 caaccgatta tataagaaga tgtcttttag ggtaagagtg aatttaaaat ggtaaaacca
    35521 gtttatattt attgtgatga aggtagcagt gaaattggtg ttcaatcact gttattggca
    35581 gtaaaacagc atttacagcg tccggcttat ctcatcaatg ccaaacaaat tattcaaagc
    35641 aagttttccg atggctgttt attaatcatg cccggtggag ccgatttacc ttactgcaaa
    35701 aaattaaacg gagaaggcaa tcgtagaatc cgccaattta ttgaacaagg tggagcttat
    35761 ctcggtatct gtgccggagc ttattatggc tgtgctgaat tgaattttgt agggcgtgac
    35821 tatagcgtaa atggtaatcg agaactagct ttatttaatg gcattgcggg cattgcgaaa
    35881 ggcagtctgc cgcagtttac caacgggcaa ctttacgata aaaacattgg tagcaaagcg
    35941 atagttgaag taacacttgc agagggtgga aaaatgccgt tttattatca cggtggttgc
    36001 tgttttgaac caaattcaac aacagtgatt tacaacccgt tggcttatta tcctgatggc
    36061 tcagtagcaa tgatagaggg agagctaggg cgaggttact atctgttgtc gggtgtacat
    36121 tttgaattac aagcggtcgt ttattggcaa aaaattgtaa attgctgtga aaatcttgac
    36181 ataaaaacac aagaagaagc aatttgctct aattttaatg taaattatgg ctcgcaaatt
    36241 tggcaacatc taaagcaacg actaaatcaa gcgtaaaaaa ataacctttc gctattctgc
    36301 caaatggttt ctgcaatcag atttggtgat tctgttctta attcacatag actattgaaa
    36361 gtcactaaaa gccgttccgg tcggttcggc tggccttgaa agccaaacac cggcatatcg
    36421 ggcgagtcgg tttccagtag taaactttca agaggtagtt tgcggatagc ctcacgggtt
    36481 ttgttagcac gttcataggt aatagttccc cccacaccaa ttttataacc gagatcgaca
    36541 aaacgttttg cctgttcata gctgccggca aagccgtgaa ccacaccggt tttggttagt
    36601 tgggcttttt tgaggaacac aaaaagttga tcgtggcttt tacgtgaatg aagattaacg
    36661 ggtaaattaa attttttcgc aagggaaagt tgtgcctcta aaaattcaca ttgctttttc
    36721 cataattcag gggtagaaac ttcacttacc gctttttcca aaccgatttc ggcaatagcg
    36781 gtacactgtg gatcttgttg gcttagcaaa ttttccaata agattaaatc ttgttgttgg
    36841 tgctgttgaa tatacagtgg atgcaaccct aatccataca ccaaattttc cggtttttgt
    36901 ttggcacaag cggtcacttt tgcaaaattt tttgcaaata cggcaacgat aagaatgcat
    36961 tcaacctgat gctgtttggc attctcaacc aattccggaa tgcttaaatt caggctttct
    37021 gcgagatagt cgaggtgagt atgagtgtca aaaaatcgca taataaaacc aataaaaacg
    37081 gggactagat tgtccccatt tttagcattt tacgcaagca attagtttgt tacataagca
    37141 tcttcatcac gagcctcaga tggcttgagg aaagcaaaga agcaaataac acaaactacc
    37201 gtaatcgcaa aaccgatata aactgataat tcataatcta aaccgaagcc gattttgttg
    37261 ttcgcaaggt aagttgcaca cacggtagtg ataaaccatg caggaatcga gcatacccag
    37321 tggaattttt tatagcggta taagtaacct gcggcagtcc ataacataac cattgcagtc
    37381 atttggtttg cccaagtgaa gtaacgccat aatacgctga aatcaacttt tgagacaatg
    37441 aaaccaagta cgaataatgg tacggcaata tacaaacgtt ttactaaggt acgttgttct
    37501 aaaccaaaga tttccgctag ttgtaaacga gcggctcgga atgcagtgtc acctgaggtg
    37561 attggtaata ctacaacgcc taatacggca aaaataccac cgataaaccc taagaagtgt
    37621 aacgagctgt catataccac tttagacggt gaaccggcag agatcgcatc ttgtaaggct
    37681 tgtgggtttt catagaatga aagacctact gcacaccata ctaatgcaat taccccttca
    37741 gtaatcattg caccgtagaa aataaaacga ccttctttct cattttgcgc acagcgagcc
    37801 attaatgggg tttgggttgc gtggaaaccg gataatgctc cgcaagaaat agttaagaat
    37861 agtaatggcc aaattggaac atcacctttc acttggaagt tttgggtgaa tttttcccac
    37921 gttaagcctt gaccgtctgc attcactgta cggaagaagt cgataggatc agtcgcactt
    37981 aagtgagcgc tgactagacc atataccata cctacagaca taaagagtaa taatgctccg
    38041 aagaaaggat aaattcgacc gatgatttta tcaacaggta gtaatgtagc aagaatatag
    38101 taagcaaaaa tgattaacgt ccacacagaa atcacggttg ctttatccat accccaaacg
    38161 gttacgccac ctgatgctac agattcttta attgcttcaa catcacctaa ttccattgca
    38221 ctttgggttg cacctgcaat atccatggtg atggtagaga gtaactgagc agggcttgca
    38281 acgaaaacta cgcctactaa taaaagcaag atgagcgcca ttacgttgat gaataacttg
    38341 actggacgac ccaagaattt ccctgccaga tttggcatcg ttgcgccacc gttgcggata
    38401 cttaacatac cacagaagta gtcgtgtact gcaccggcaa aaatacaacc aatcacaatc
    38461 caaagcattg caactggtcc gtataaggca ccaagtattg gtccaaaaat agggccggta
    38521 ccggcaatgt ttaataattg aatcagccaa atcttggttt tagacattgg catatagtca
    38581 acaccgtcgt tcattgcata agccggcgtt gctcttttcg ggttaatcac gaagattttt
    38641 tcaatgattt ttccgtaagt aaagtagcca agaacaagca cggctacaca gaaaaagaac
    38701 cacaacatag tagaaatcct ataaattgag ataaatgatc atttttagca gaactgataa
    38761 aggcgtgcca ttgtaagtga ggagtgagga taaagtaaaa tgaattgtta gatttgtgtg
    38821 aataatttac tgaaaatgtg aggttcatca aaaaatccct ttgttctgcg tcaatcaaag
    38881 ggacttttta aagttaaata tcgaattttt gaattttcgc ttcaatgcct tgggcatcta
    38941 atccaagatc ggcataagct tcagcttgag tagattgggg aacgaaaaag tcaggaatac
    39001 caagcattac aagcggtttg attttgccga tattttgcaa atattcattc acaaaactac
    39061 ctgcgccacc ttgaatagca ttttcttcaa gcgtaactaa caactcgtgt gaatccgcca
    39121 ctttttgaag taatgcttca tctaatggtt taatgaaacg catatctacc aacgtgtagt
    39181 tatgtttttc agccacaatc tttgcttcat caagcaaagc accaaaattt aaaatcgcaa
    39241 cttttttacc ttcacgaata atgtttcctt tacctacttc aagtctttgc attggctcaa
    39301 gttttacccc tttagcattg cctcgagggt aacgaatagc acttggcgta ttcatcgtgt
    39361 aagcggtata aagcattttt cgcatttcat tttcatcgct cggtgccata atggtcatat
    39421 tgggaataca acgcataaaa cttaaatcaa atgcaccttg gtgagtttgc ccgtcagcgc
    39481 cgacaattcc ggctcggtca atcgcaaaaa tcaccggcag gttttgaata gcaacatcgt
    39541 gaattaattg gtcataagct cgctgcaaga agctggaata aattgcgact accggtttgt
    39601 aacctgcgat tgctaagcct gccccgaaag tcactgcgtg ttgttcggca atcgccacat
    39661 caaaatattg ttcagggaaa cgtttggaaa attctaccat tcccgagcct tcacgcattg
    39721 ccggcgtaat gccgataatt ttcggatcac gctctgccat ttcacatagc caatcgccaa
    39781 aaatattgga ataagtaggt gctgcttttg attttggtaa ctcggctgcg gtagggtcaa
    39841 atttcggaac gccatggtag ccaatcggat cattttccgc aggtgtgtag cctttgcctt
    39901 ttttcgtgcg aatatgcaga aattgagggc ctttcagctc acgcatattg ctcaaggttt
    39961 tgactaactc gtcaatatta tgtccatcaa tcgggccgat ataattaaag cctaactctt
    40021 caaacaaagt gctttcaggg gagaaaatca cccctttcat atgctcttcg ctttttttca
    40081 taaagttttt gatggtaggc actttatcta aaattttctt actgccgtca cgcactgaag
    40141 tatagagcga accagaaaaa atgcgggcaa ggtggttgtt taatgccccg acattttcag
    40201 agattgacat ttcattatca tttaagataa ccaacatatc ggtatgcaat gcacctgcgt
    40261 ggtttaatgc ttcaaatgcc attccggcag tgattgcacc atcaccgatt acacagacgg
    40321 ttttacggcc ggcattttct ttttctgctg ccaccgcaat gccaagtccg gcactgatcg
    40381 aagtagaaga atgcccaacg cttaacacat cgtaagggct ttcttcacgc caagggaagg
    40441 ggtgaatacc atctttttgg cggatagtgt gcatttgatc tcggcgccca gtaaggattt
    40501 tatgtgggta agcttgatga ccaacgtccc aaattaattg gtcgaaaggc gtttgataaa
    40561 cataatgcaa agctaccgta agctctacta cccctaagcc agaggctaaa tgtccgctag
    40621 tttgactgac tgattccaat aaataggctc gcagttcatc aactacggtt tgaagctggt
    40681 ctttagaaag taaacgtaaa tcttccggtg agttgatttc gcttaacaga ggatacttct
    40741 tttccataat gttttattac ccacaaccat caaataatag atgatattaa attaagcttt
    40801 gagttcttcg gtcaaagtag gctgaatgct atttaaaatt tctttaacca cacggccgtt
    40861 accaacaacc agattacctg ttttcaagta atctgttccg ccaacaaagt tagtcacaat
    40921 tgcaccggct tcacgggcaa taagttcacc ggcggcgaaa tcccacggtt ttaagccgat
    40981 ttcaaagaaa ccatcaacac gacccgctgc aacataggca agatctaaag cggcagaacc
    41041 ggtacggcgg aaatcagcta ctccgttatt cattaagcct tcaagcatat ttaaatgtgc
    41101 atcacgatgt ctgatgactt tgaaaggaaa accggttgca attacagtgc cttttaaatc
    41161 acggcgttct gcatcaatgc gtaaacggaa ctcgtttaat ttagcccctt cgccacgcac
    41221 cgcagtgaaa agctcgttac ggataggatc gtaaactacc cctactgttg tacgtccgtt
    41281 ttcacgcacg gcaatcgaca cagcaaaatg aggtaagcgt tttacgaaat tggttgtacc
    41341 atctaatgga tcgatcaccc attgtacatt ttcattgcta ccgtttaaca cgccggactc
    41401 ttcggtcacg atcgagtgtt cagggtaaga tttacggatc acatcaataa ttgccgcttc
    41461 cgctgcttta tcgatttcag tcacataatc attcgtattt ttttgtaaaa cttgtgtatc
    41521 ttgaggcgat tgttcatagc ctttggcaat aatattgcct gcttttcgtg ccgcacgaat
    41581 agcaatgttt aacattgggt tcatatttcc accagatttt aaagaacata ataaagcacg
    41641 aattataaag aatgtagcga aataaagaaa caacaagcgg tctaatctgt gaaaaatttt
    41701 gcaaaaaaat ggctttttct aaccgcttgg agtttggatt cgttataata cctcccattc
    41761 aagccaccca tttaggtact aaaaatgcaa tttaaagaaa ataaccaacc tcgttttaaa
    41821 cacagagaag aacgccgttc agatgagtgt tccccccgtt tttcagataa aggcaaaatg
    41881 cgaggaaatg aaggtaaaag tgacagaccg cataagctac attttaagcg tgacgaggct
    41941 tattcacgct ctgagcagat ttatccagca gaaggcaaaa aagccagtgg tgaaggtagt
    42001 gtgcaaattt gggtaaaagg tgggaatagc tcagttaaag agaaaaaaaa cggcccgctt
    42061 tcaccgagag cgcctgagaa aattaagaaa aatcgcttgg aagagatgaa agtctatggc
    42121 gaaaatgcct gtatcagttt gttccaacaa cgtcctgaat ctattgtacg tttatgggca
    42181 acagtggagg gggcaaaaaa attaggcgat ttaatgagct atttggctca gcacaaaaaa
    42241 gcctatcacg ttattgaccg tgaagagatg gagcgagtca ccggtactga acatcacggc
    42301 gatatgtgct tattggtgaa aaaaacggct tcatttgtgc tagagggcta tttacaagtg
    42361 ccgaaaaaac aagattgctt ggtactgtta gatggtgtaa acaatgctca gaatgtgggg
    42421 ggaatcatca gaacgtgtgc tttttatggt gtgaaaggca ttattactga aaatgtggat
    42481 agcttaaact ccagtgctgc agctcgtgtc gcagagggtg gtttggaatt tgtgcgagcg
    42541 ttagaaacta aaaacaagca tattgctttg gtacaactcc gccaagccgg ttatcaaatt
    42601 gtccacctca cacgccataa gcaagcccct tctttaacca aaattaagct ggcggaaaag
    42661 gtggtttttg tattaagtga gattcctgca agtgatgtgg cttacgcaga agacacaaca
    42721 atacagcttt cctttaataa tccgttaaat agtggtttaa atgtggcggt aaacgcaggt
    42781 attctactta atcaatggta tcaaacccac attctttaag gaattgaaat gaatcagcaa
    42841 aaaccgtttg aagtgattgc gattgttggc acacctcgcc acgacagtgc atttgaaaca
    42901 catcttgccg tttataattg gttaaaagat cgtgattatc aaattttagt ggaaacagag
    42961 gttgccactc agttacagct taaggaaagc tacagtttag atcagattgg gcagcgcgca
    43021 gagttggtga ttgtgatagg tggcgatggt aatatgcttg gcatcgcccg agctttggct
    43081 aaataccgag tgccactgat aggcattaac cgtggcaatc tgggtttttt aaccgatatc
    43141 gcacctcaaa cggcttttga acagctttat agttgcttag tcaaaaatga gtatgtgatt
    43201 gaagagcgat tcctgttaga ggctttcatt gagcgcaacg gtaaaattat tgccgcaaat
    43261 aatgccttga atgaaattgt ggttcaccca acccaagtgg ctcgaattat tgaatttgaa
    43321 gtttatattg acggaaaatt cgctttttca caacgtgcag atggcttgat tgtggcaacg
    43381 cctacaggct caaccgccta ttcactttct gctggtggcc cgattttaac cccgaatatg
    43441 aatgcaatgg cattagtgcc gatgcaccct cacactcttt cttctcgtcc tttggtggtt
    43501 gatggcgata gtcaaatttc gcttcgtttt gccaaatata atacccctga tttagaagtg
    43561 atttgtgacg ggcaggtgaa tatgcacttt acgcctgaag atagagtctt ggtgcgtaaa
    43621 agcccggata aattacagct gctccattta aaagattata gctactttac tgtattaggt
    43681 tctaaattag gctgggcaag taagttgttt taaacacaag cggtgttttt tctgattaaa
    43741 tttg
//
LOCUS       DANLXQ010000003        41975 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.003, whole genome shotgun sequence.
ACCESSION   DANLXQ010000003 DANLXQ010000000
VERSION     DANLXQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 41975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41975
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.003"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            141..437
                     /locus_tag="PXD94_000110"
     CDS             141..437
                     /locus_tag="PXD94_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL6285020.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
     gene            427..843
                     /locus_tag="PXD94_000111"
     CDS             427..843
                     /locus_tag="PXD94_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL6285021.1"
                     /translation="MHSDYLVIDTNILISGLLFPKSNPNLAVKKALLGYTICVSQATF
                     DEFLEVSARPKFAKYFSIQEREEFIANLLDAVKFVEIKETITDCQDPKDNKFLEVAIS
                     AKAKYLVTGDKKDLLSMNPYRNVEIISVSEFLAETR"
     gene            843..2501
                     /locus_tag="PXD94_000112"
     CDS             843..2501
                     /locus_tag="PXD94_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL6285022.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHCKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            2587..3244
                     /locus_tag="PXD94_000113"
                     /pseudo
     CDS             2587..3244
                     /locus_tag="PXD94_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(3228..4166)
                     /gene="ttcA"
                     /locus_tag="PXD94_000114"
     CDS             complement(3228..4166)
                     /gene="ttcA"
                     /locus_tag="PXD94_000114"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL6285023.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLYEDDDNNFAEAGMIQFKEVN"
     gene            complement(4229..4942)
                     /gene="ubiG"
                     /locus_tag="PXD94_000115"
     CDS             complement(4229..4942)
                     /gene="ubiG"
                     /locus_tag="PXD94_000115"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL6285024.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            5007..5387
                     /locus_tag="PXD94_000116"
     CDS             5007..5387
                     /locus_tag="PXD94_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL6285025.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            5484..8180
                     /gene="gyrA"
                     /locus_tag="PXD94_000117"
     CDS             5484..8180
                     /gene="gyrA"
                     /locus_tag="PXD94_000117"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL6285026.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDFAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETAETSTEE"
     gene            8275..10239
                     /locus_tag="PXD94_000118"
     CDS             8275..10239
                     /locus_tag="PXD94_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6285027.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELAPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENAGYEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRV
                     DLDPEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNCLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFAATADKRLNSSKNLQKIEEKAPLAEQEKQKAD
                     QEKTKAEPTKKTKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNA
                     QLQKLADTEAALEQAFERWEALENIKNGNI"
     gene            10321..11079
                     /gene="tcdA"
                     /locus_tag="PXD94_000119"
     CDS             10321..11079
                     /gene="tcdA"
                     /locus_tag="PXD94_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL6285028.1"
                     /translation="MTERLDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWA
                     VEALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVE
                     IIDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDP
                     TKIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEV
                     SATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            11126..12493
                     /locus_tag="PXD94_000120"
     CDS             11126..12493
                     /locus_tag="PXD94_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL6285029.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDSVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            12513..14060
                     /locus_tag="PXD94_000121"
     CDS             12513..14060
                     /locus_tag="PXD94_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL6285030.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMGQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            14283..15317
                     /gene="metN"
                     /locus_tag="PXD94_000122"
     CDS             14283..15317
                     /gene="metN"
                     /locus_tag="PXD94_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL6285031.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            15307..15984
                     /locus_tag="PXD94_000123"
     CDS             15307..15984
                     /locus_tag="PXD94_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6285032.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLVVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            16007..16840
                     /locus_tag="PXD94_000124"
     CDS             16007..16840
                     /locus_tag="PXD94_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL6285033.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARALILLEKQ
                     GLIKLKDNTNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYAGQVGLN
                     TQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFKDGVVKG
                     W"
     gene            16923..17753
                     /locus_tag="PXD94_000125"
     CDS             16923..17753
                     /locus_tag="PXD94_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL6285034.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVAVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            17805..18596
                     /gene="metQ"
                     /locus_tag="PXD94_000126"
     CDS             17805..18596
                     /gene="metQ"
                     /locus_tag="PXD94_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL6285035.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQDLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGAMIKGW"
     gene            complement(18641..19792)
                     /gene="metK"
                     /locus_tag="PXD94_000127"
     CDS             complement(18641..19792)
                     /gene="metK"
                     /locus_tag="PXD94_000127"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL6285036.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMEQQSKVRKSGKLDW
                     LRPDAKSQLTFAYENNKIIGIDAVVLSTQHAEHISQKELIEGVMEEIIKPVLPSEWLS
                     QNTKYFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKVSNEILVKLIYQNF
                     DLRPYGLIKMLDLIKPIYRETAAYGHFGRKHFPWEKTDKAEALRAGAGL"
     gene            complement(19975..20817)
                     /locus_tag="PXD94_000128"
     CDS             complement(19975..20817)
                     /locus_tag="PXD94_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL6285037.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLDPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNVFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            20877..21194
                     /locus_tag="PXD94_000129"
     CDS             20877..21194
                     /locus_tag="PXD94_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL6285038.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLFFDNKIVAIISKD
                     YRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELAIEEADELVKWIRLGL
                     QAV"
     gene            21285..21998
                     /locus_tag="PXD94_000130"
     CDS             21285..21998
                     /locus_tag="PXD94_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL6285039.1"
                     /translation="MNQKKLVSLIVVIVLAIISQIFSFFDKKEPSKAQEKSTQSSQSH
                     QVSTSSLGNYDVQMKNDRLKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQ
                     CGGSADFGWVIHGLWPQSGKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQG
                     EWEKHGACAFNQADDYFAKQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGS
                     KDELYICYDKAFQPMDCPR"
     gene            21998..22492
                     /locus_tag="PXD94_000131"
     CDS             21998..22492
                     /locus_tag="PXD94_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDL6285040.1"
                     /translation="MADLRQLKMQVQRQLKRDLERANHYFNTTFTPPSISYAVRGVKA
                     GVAYLERNEVRFNPVLLQENEQAFIRQVVPHELAHILVYQHFGRVQSHGKEWKMMMET
                     VLGVPAEIYHCFDTQSVQQQFSYQCACQTHQLSIRRHNAVMRDKKSYICRLCKTQLHF
                     VEKS"
     gene            22597..23877
                     /locus_tag="PXD94_000132"
     CDS             22597..23877
                     /locus_tag="PXD94_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL6285041.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDESVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIEGKEMKPFRFNDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(23964..25001)
                     /locus_tag="PXD94_000133"
     CDS             complement(23964..25001)
                     /locus_tag="PXD94_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6285042.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPRSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKAEALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
     gene            25223..25921
                     /locus_tag="PXD94_000134"
     CDS             25223..25921
                     /locus_tag="PXD94_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL6285043.1"
                     /translation="MENSWKYAFKLITPIFMGYFAAGVAYGMLATNAGMPAWFTIVMS
                     FTVVSGTAQYAAIPFFVSGVGAVSVFLSTLLMSLRFSFYTLNMMEHKPISKAKSLVAI
                     AGLTDEGFAVLSSLPKEQKQALFFKIAVLCVAYWTFSTIVGVLLGDSVANYIPHLDFA
                     LPCLFAILAYEQYKNQKQWKPIVIALIGFLLARQITETSVLLVAILVAIVIVALLPQS
                     SSIKLEKGVENEHK"
     gene            25932..26234
                     /locus_tag="PXD94_000135"
     CDS             25932..26234
                     /locus_tag="PXD94_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL6285044.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTFLSVALGIFGVNVFLSLLG"
     gene            complement(26355..27656)
                     /locus_tag="PXD94_000136"
     CDS             complement(26355..27656)
                     /locus_tag="PXD94_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL6285045.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVGLIFDPNVKYTGFFK
                     MPSFGENSQFLNLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(27789..28763)
                     /gene="secF"
                     /locus_tag="PXD94_000137"
     CDS             complement(27789..28763)
                     /gene="secF"
                     /locus_tag="PXD94_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL6285046.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGDMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(28773..30614)
                     /gene="secD"
                     /locus_tag="PXD94_000138"
     CDS             complement(28773..30614)
                     /gene="secD"
                     /locus_tag="PXD94_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL6285047.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSGLQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(30654..30947)
                     /gene="yajC"
                     /locus_tag="PXD94_000139"
     CDS             complement(30654..30947)
                     /gene="yajC"
                     /locus_tag="PXD94_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL6285048.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
     gene            31148..31705
                     /locus_tag="PXD94_000140"
     CDS             31148..31705
                     /locus_tag="PXD94_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL6285049.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(31746..32714)
                     /locus_tag="PXD94_000141"
     CDS             complement(31746..32714)
                     /locus_tag="PXD94_000141"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL6285050.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(32840..34003)
                     /gene="galK"
                     /locus_tag="PXD94_000142"
     CDS             complement(32840..34003)
                     /gene="galK"
                     /locus_tag="PXD94_000142"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL6285051.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(34013..35059)
                     /gene="galT"
                     /locus_tag="PXD94_000143"
     CDS             complement(34013..35059)
                     /gene="galT"
                     /locus_tag="PXD94_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL6285052.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            35272..36123
                     /locus_tag="PXD94_000144"
     CDS             35272..36123
                     /locus_tag="PXD94_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HDL6285053.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKTSSNIQSWAYPDFAYTKLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPY
                     WLATLTALMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFF
                     LIGFFIWLAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQ
                     TVRVSSD"
     gene            complement(36209..37294)
                     /locus_tag="PXD94_000145"
     CDS             complement(36209..37294)
                     /locus_tag="PXD94_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL6285054.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(37316..38296)
                     /locus_tag="PXD94_000146"
     CDS             complement(37316..38296)
                     /locus_tag="PXD94_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL6285055.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(38399..39497)
                     /gene="prfB"
                     /locus_tag="PXD94_000147"
     CDS             complement(join(38399..39421,39423..39497))
                     /gene="prfB"
                     /locus_tag="PXD94_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL6285056.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            39753..40661
                     /locus_tag="PXD94_000148"
     CDS             39753..40661
                     /locus_tag="PXD94_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL6285057.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTW"
     gene            40962..41603
                     /locus_tag="PXD94_000149"
     CDS             40962..41603
                     /locus_tag="PXD94_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL6285058.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            41770..>41975
                     /locus_tag="PXD94_000150"
     CDS             41770..>41975
                     /locus_tag="PXD94_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL6285059.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERP"
BASE COUNT        12173 a         8529 c         9371 g        11902 t
ORIGIN      
        1 aatagaaaat caagtgtttg ttcaagtttt tatgttattg aatttacaat aactacaaaa
       61 atttatctta attggctcga ttgattttta cacaaaacat agctaacata tccaaaatca
      121 ctcacattag gagcttaaaa atgacagcga ttactatccc tcaatttgaa gaggtgactt
      181 ctaagcaatt tcaatctgag ttcggcagaa ttgttgatat cgtaaaaggc aaaacccctg
      241 tggcgattac ccaatatggc agaccgacaa tggtgctttt tccttacgat caagcggttg
      301 aagctcggcg attattagca aaacagcgtt tgcttgaaat gcttgcagag cgagataaga
      361 atccgccggc agctcttgca gaaatcacgc ttgaagagtt ggataaaatg attgatgagg
      421 aacgtaatgc actctgatta tttagtgatt gatacgaata ttttgattag tggtttattg
      481 ttcccaaaat caaatcccaa tttagcagtg aaaaaagcct tattaggcta taccatttgt
      541 gtctcgcaag cgacttttga cgaattttta gaagtctccg ctagaccgaa atttgctaaa
      601 tatttcagta ttcaggaaag ggaagaattt attgccaatt tgttggatgc agtaaagttt
      661 gtggaaatta aagaaacgat tacagattgc caagatccca aagataataa atttttagaa
      721 gtggcgatca gtgcaaaagc gaagtattta gtgacgggcg ataaaaaaga tctcctcagt
      781 atgaaccctt atcggaatgt tgaaattatt tccgtcagcg aatttttagc ggaaactcgc
      841 taatgaaacg gtttctcact ctttttcttc tccttttttc atctttttca ttagccgaaa
      901 cccaccaaat cggcattctc gctcagcgtg gggaagccta cacccgcacc cattggcagc
      961 cttgggtgga ttggttaaat tcgcagtttc ccaacgagcg gtttgagctg gttccattgg
     1021 ggattaacga ggcgagccgc cgggcggagt tggattttct gctgaccaat caagctcagt
     1081 ttttctattt gagtaaccaa aatgtgcgat ggcttgccac gctcaacagt ccgttttcgg
     1141 caaatggtga gcaaggggcg gtgggcagta gcatttgggt gcgtgccgac agccattatc
     1201 agcagttaag cgatctgaaa aatcagacta tcagtgcggt ggataacgat gctttcggcg
     1261 gttttttgct tgggttgtat cagtttcatc aagcaggaat gcggcaaaat cgggattttt
     1321 cggtgcagtt tagcggtttt ccggtggaaa atgcgttggc tttgctggcg gaaaaacagg
     1381 tggaagcggc gattgtaccg gtctgtttgc tggaagagtt ggagaaagag ggcaagttca
     1441 agcggtcgga ttttcgctta attttgcaaa acccacaggc gcagggttgc cttgccagca
     1501 cgccgttgtt gccgaactgg tcgctggcgg caatggaaaa tgtgccaaac gagctggcgg
     1561 tacagcttgc cacacgcttg ctaaacagcc acaatcccga cctaccccgc tggacgttgc
     1621 cgttttcttc cgcccaagcc gatcacattc tgcgtgaact gtatcgccac ccgcaacaaa
     1681 aaagcctgtg ggcaacggtg ctggattggg ttcgtctcaa taaattcggc ttgttggcgg
     1741 tggtgttgtt cattttgctc aaccttgtcg ccctgcgtta tcaggtctat cgcaaaagca
     1801 aggcgttaca gcaggcacac tgcaaaatgc agcagtatca gcaggaactg acccgagccg
     1861 accgccttgc tttgttgggc gagatgacaa ccggttttgc tcacgaactg aaacagccgt
     1921 tgtcggcggt gcgaatgtac gccgaggggc tgaaaagcca aaacagcgat ccttatcaac
     1981 agcggatttt ggataagctg attgcgcagg tagatcgggc agtgaaaacg atgcagagca
     2041 ttcgggactg ggcgcaaaac cgcccgagtg gtgaaccgca agcggtcatt ttgaaccaat
     2101 tgattactaa tgtgatcgag tttgtggcgg tggaaaatcg ccagaatgca caaatctcgc
     2161 tgattgccga ccgcactttt cggcttaatt tacacgccac ggtactggaa caagtactga
     2221 cgaactgcct gctcaacgcc ctgcaagccg gtgcaagcga gatatcggtg cggttacaag
     2281 cggtcgaaaa cgggctggaa attgcaatcg aagacaacgg cggcggtttt tcccctgccc
     2341 agcttgaatt tccgtttgtg ccatttcgca ccgacaaacc acacggctta gggctaggct
     2401 tggtgctatg ccaacggctg atgcaatcgc tcaacggacg gattgtattg gcgaacgggg
     2461 agaacggggc gaaggtagtg cttttactcc ctcaaatata aatgtagggg caggtcctgt
     2521 gcctgcccgt aaatcctatc aaattcaggg cgggcacagg acccgcccct acggaaaaaa
     2581 gaaaaaatga ttattcacat tcttgatgac gaagaaagca ttttagacgc tatgtcgttc
     2641 ctgcttgccc cgcttggggt ggaaatccaa acgtggcaat ccagcctaga ttttttggca
     2701 caggcggatt tacaccggca aggcgtgctg ttactagata ttcgaatgcc gctgccagac
     2761 gggcaacagg ttcatcaaca actgcgagag gcacaatcga ccctggcggt ggtgattatg
     2821 accgcccacg gtgatgtgcc gatggcagtc gctgaattga aaaaaggcgc agtggatttt
     2881 ctgcaaaagc cggcaagttt tgaacagcta aaacaggcga ttgcacaggc ggaaaaagta
     2941 agtgaaaaag cggtgaaaat tcgtgaaaat tcgtgaaatt tcgcaaaatt acgccaaatt
     3001 gacggaaaaa gagcgtaacc ttgtgccact gattatgcaa ggtttcacca ataaacagat
     3061 cgcagataaa ttagcgattt cagtgcgtac ggtggaagta catcgagcca atgtgatgga
     3121 aaaaatgcag gcggagagtt tggcggggct ggtgcagatg atcggggagt tacaatataa
     3181 aggtagcgga aattgctctg ctacctttag tttcgacttt tgccgtatta atttacttct
     3241 ttaaactgaa tcattcccgc ttcggcaaaa ttgttatcgt catcttcgta taagaaggtt
     3301 ttggcttcaa ttttgacatc atcaaaagcg gtgtcgcctt ctatgccttc aatggtttga
     3361 ccgtgtttaa tccctttaaa atcaaacaat ttcggatcgc ataagtggga tggcacgata
     3421 tcttgcaaag cactgaacat tgtttctaaa cgccccgggt attggcgatc ccaagtattc
     3481 agcatttctt tcaccacttg gcgttgtaaa ttaggctgag aaccacacag attacacgga
     3541 ataatcggga actgcttggc ttcggcatat ttgataatgt ctttttcttt acaataggcg
     3601 agtgggcgga tcacaatttg cttgccgtca tcagagatca gtttaggtgg catacttttc
     3661 attttgccgc cgtagaacat atttaagaat aaagtcgcca gcatatcgtc acggtgatgc
     3721 cctaatgcaa ttttggttgc acccaattcg gtggcggtgc ggtataaaat cccacggcga
     3781 agtcgtgagc aaagtgaaca ggtggttttg ccctccggaa ttttctcttt tacgatgccg
     3841 taggtgtttt cttccacaat tttgtattcc acgccaatgc tttctaaata gcgaggcaat
     3901 acctcttccg gaaatcccgg ttgcttttgg tctaaattga ccgctaccac actaaaattc
     3961 accggtgcac tttgctgtaa attgagcaaa atatctaaca gcgtatagct gtctttaccg
     4021 ccggaaaggc agaccattac acggtcgccc tcttcaatca taccaaaatc ggcaatggca
     4081 ttaccgacat tacggcgtaa acgtttttgt aacttgttga aattataagt aattttttta
     4141 tcagattttt gaatatcgtc tgtcataata gttgtactag gcttagaaat tagcggtgta
     4201 tagtaaagat ttcgcactat tttgcaagtt aattattcgg cttcagcgta gcgatatagt
     4261 tacaactcac atcattattg agccaaaatt tttcggttaa tgggttgaaa tggtagcctc
     4321 tcatctcttg gcaacgtaaa tcagcctgat cgcaccaagc taacaattcg gcaggcttga
     4381 tgaatttttc aaattcgtgc gtgccttttg gcaacatttt cagcacatat tccgccccga
     4441 taattaccaa catataggct ttaaaagtac ggttgatggt agagaaaaac agcacgccgt
     4501 caggtttgag cagggcttta cagctttgaa tgatagaaag cggatcggga acgtgttcca
     4561 gcatttccat acaagtaatc acatcgaatt tttcttgatt acaagcggtc atttttgcta
     4621 aaaaattttc aatcgtggtt tgttggtaat caatctctaa cccactttct ttggcgtgct
     4681 gctttgccac ttcaagcggt tgagaggtca tatcgatgcc ggtaacaatc gctccctgtt
     4741 ttgccattgc ctcacttaaa ataccgccac cacagccgac atctaacacc tttttgccaa
     4801 ataaaccgtt cgatttttct aaaatatagg ctaaacgcag tggattaagc agatgaatcg
     4861 gcttgaaact gccgtttgga tcccaccagg ttttagccat attttcaaat tttgtgattt
     4921 cttgctgatc gacgttttgc ataaactgtt cctatggtaa atttagggca ttactataac
     4981 gcatttagcc taaaaggaaa atcagaatga cccgaattaa ccttgttccg ccaacggaat
     5041 tatgcgatca acatctgctt gccgaacatc gtgaacttac tcggattccg aatgcggtag
     5101 cgaaaggcaa atttagccta aaaggacagc cagaagatta caaactgggc gagggacacg
     5161 ttcggttttt cttcaataaa ctcacgtttt tgaaaaagcg ttacgatgcg ttacacgagg
     5221 aatgtttagc aagaggtttt aaggtgcaat atttctgggc ggaacattta ccacaagatc
     5281 cctctctctg gctagattat cagccgactg aaaccgctct gactgtaaat cgggagcgaa
     5341 ttgcattaag aatgccggca aatccacgtt ttagcgaaag aaaatgaccg cttgtacgcc
     5401 tttttgttgc tttttatgct ataatctcgc caattttttc gccttattta aggctctttt
     5461 actaataaaa ttaggaaatt tcaatgagcg aattagccaa agatattatt cccgtaagta
     5521 ttgaggacga attaaaaacc tcttaccttg actatgcgat gtcggtgatt gttgggcgtg
     5581 ccttgcctga tgtacgagac ggtttaaagc cggtacaccg ccgagtgctt ttttcaatgg
     5641 atcaaaacag caacacctat aataagccgc acgttaaatc tgcccgtgtg gtcggtgatg
     5701 tgatcggtaa atatcaccca cacggtgact ttgcggtgta taacactatt gtgcgtatgg
     5761 ctcaaccgtt ctcactacgt tatatgttgg tggacgggca aggtaacttt ggttctattg
     5821 atggcgatgc accggcagca atgcgttata ccgaagtgcg tatgcagaaa attacgcagg
     5881 aattattaac cgatctcgat aaagaaacgg tggatttctc accgaactat gacggcaaag
     5941 agatgattcc tgatgtattg ccaaccaaaa ttccggctct gttagtcaat ggttcttcag
     6001 ggattgcggt cggtatggca accaatattc cacctcataa tttaggcgaa gtattagatg
     6061 gttgcttggc gtatattgaa aatgaacaga ttagtgttga agagctaatg cgttatatcc
     6121 cggggccgga tttcccgacc ggggcgatta tcaacggtcg taaagggatt gaagaggctt
     6181 accgcaccgg tcgtggcaag gtgtatgtgc gcgcgaaagc cagcgtggaa acaactgata
     6241 aagggcgtga gcaaattgtt gtaaccgaac tgccatacca agtgaataag gcaaaattaa
     6301 ttgaaaaaat tgccgagctg gttaaagaga agaaaatcga aggcatcagc aaaattgatg
     6361 actattccga taaaaaaggg atttcgattg ttattgaagt caagcgtgat gcagtgggtg
     6421 aagtggtgtt aaaccacctt tattctttaa cccaaatgca ggtcacgttt ggtatcaata
     6481 tggtggcgtt agataatggg cagccaaaag tgctgaactt acgccagatc atcgaagcct
     6541 ttgttaaaca ccgtcgtgaa gtggtcacac gccgtacgat ttttgaattg cgtaaagccc
     6601 gtgaacgtgc ccatatttta gaaggtttgg cgattgcgtt agcgaatatc gacccggtaa
     6661 ttgaattaat tcgagcctca aaaaccgcag aagaagcccg tgaaggctta ttggctcgcc
     6721 cttgggcatt aggcaatgtt gcaccaatgt tagaggctgc tggtgttggt gcttcccgac
     6781 cggaagattt acctgaacac ttaggagtgc gtgatggcga atactattta tctgaagctc
     6841 aagccagagc gattttagag ctacgcttac accgcttaac cggtttagaa cacgagaaaa
     6901 tcgttgaaga atacaaagaa ctcttaactg aaattggcga attaatccac attttaacca
     6961 gcccagaacg cttaatggaa gtgatccgtg aagagttaga acgtgtaaaa accgatttta
     7021 atgatgaacg ccgtactgaa attaccgcag cctctggcga catcaacatt gaagacttaa
     7081 tcgcacaaga agatgtggtg gttacgcttt ctcacgccgg ctatgtgaaa tatcagccgt
     7141 tatccgacta cgaagcacaa cgccgtggcg gtaaaggtaa gtcggcaacc aagatgaaag
     7201 aagatgattt catcgaaaaa ctcttggtgg caaacaccca cgacaccatt ctttgcttct
     7261 caagccgtgg gcgtttatat caattaaaag tgtatcaatt accgcaagca agccgtgggg
     7321 caagaggcac gccgattgtg aatattctgc cgttagtgaa agaagaaaac gaacgtatta
     7381 ccgcgattct accgattcca aacggtgaat ttagtgccga taagtttatc tttatggcaa
     7441 cagctagcgg tgtagtgaaa aaagtttccc ttgatgcctt tagcaacgtg cgtgcaagcg
     7501 gtctgattgc gttaaaactt cgtgaaggcg atgagctgat cggggtagat attactgatg
     7561 gtaacagcga aattatgttg ttctcggcac aagggcgagt agttcgcttt gccgaaacag
     7621 cagttcgtgc gatgggacgt accgccaccg gcgtacgagg tattaaactt tctaccaccg
     7681 aaatttcaag cgaagaaatt gaagaagctg aaattattga aactgacaat gaagaggcag
     7741 aagattcctc cagcgtcagc cttgataaag accgtgtggt ttctttagtg attcctcgca
     7801 ccgaaggcga tattttaacc gtcacccaaa acggctacgg taaacgtacg gtgattagcg
     7861 aatatccggt gaaatcccga gcaaccaaag gggtcgtttc gattaaagtg aatgaacgaa
     7921 acggcaaagt ggttgcggca gttcaagtag aggaaaccga ccaaattatg ttgattaccg
     7981 atgccggcac gctggttcgc acccgagtgg cagaagtcag cctcgttgga cgaaacaccc
     8041 aaggcgtacg tattattcgc accgcagaag acgaacaagt tgttagcctt gaacgagtct
     8101 gcgagcctga agacgatgaa aatgaaagcc tagacgcatt agaaaatgcc gaaacagcag
     8161 aaacatcaac tgaagaataa tcttaactaa acaagcggtc gaattttcac aattttttgc
     8221 aaaaatagaa ggaaattcga ccgtttttgg atcatacagt agataataaa aactatggca
     8281 cttatcaatc tttccaacgc ctatttaggc tttggcgatc atcctttatt ggatcatact
     8341 gaattacata tcgaacccgg cgagcgggtt tgtttggtgg ggcgtaacgg ggcgggtaaa
     8401 tcgaccctga tgaaaattct ggcgggcgaa gttcagcttg atgacggcaa gttggtattt
     8461 gaaaaagaca ttgtcgttac acgtcttgaa caagatccgc cccgccatat tgaagaaacg
     8521 gtgttcgatt atgtggcgga ggggattgcc catcttgccg acttattgaa gcaatatcac
     8581 cacatttccc tgcaaatgca gaccgattac agcgatgaac tgctcgccaa actttcagcg
     8641 gtgcaatcgc aattagaaca cgctaacggt tggcagtttg aaaaccgcat tcaagacacc
     8701 ttgaaactgc tggaactcgc ccccgacaaa cgcctgtgcg aactctcagg cggttgggta
     8761 cgccgtgcgg cattggctcg ggcgttggtt gccgatcctg atgtattgct gttggacgaa
     8821 ccgaccaacc acctcgacat tgaggcgatt gcgtggctgg aaaatctgtt attggatttc
     8881 aaaggcagta ttatttttat ctcgcacgac cgtgccttta tccgcaaaat ggcgactcgg
     8941 attgtggatt tagatcgagg caaattggtt tcctacccaa gcaattacga taaatacttg
     9001 gaagaaaaag ccgaggatct gcgagtagaa gcgttgcaaa atgagctgtt cgacaaaaaa
     9061 ctcgcccaag aagaagtctg gatccgtcaa gggattaagg caagacgcac ccgaaacgaa
     9121 gggcgtgtgc gagcgttgaa agcgttgcgt gaagagcgta gaaaccgccg agaggtacaa
     9181 ggcacggcga aaattcagat cgacaacacc gcacgcagcg gcaaaattgt gtttgaagtg
     9241 gaaaatgccg gctatgaaat cggcggcaga accctgctga aaaatttcag caccaccatt
     9301 ttgcgtggcg ataaaattgc cctgattgga gcgaacggga tcggcaaaac cacgctgatc
     9361 aagctgttac ttggcgaaat tcagccgaca tctggttcag tgcgttgtgg cacaaaattg
     9421 gaagtggcgt acttcgacca ataccgagtc gatcttgacc ctgaaaaaac cgtgatggac
     9481 aacgtggcag acggcaagca agatgtagaa gtaaacgggg tgaaacgcca cgttcttggc
     9541 tatctgcaag acttcctgtt cccgccaaaa cgggcgatga caccggtgaa agccctctcg
     9601 ggtggtgaac gcaactgctt gttgctggca aaactgttac tcaaaccgaa caacttgctg
     9661 attttggacg aaccgaccaa cgatttggac gtggaaaccc tcgaactgct ggaagagatg
     9721 ctcaccgact atcaaggcac cctgattatt gtgagccacg accgtcaatt tgtggataat
     9781 accgtaaccg aatgctactt cttcgagggc gaaggcgtgg tgaataaata tgtcggcggc
     9841 tatttcgatg ccaaacaaca gcaggaaaat tacttcgccg ccactgccga caagcggtta
     9901 aattcgagta aaaatttgca aaaaatagag gaaaaagcac cgcttgcgga gcaagagaaa
     9961 cagaaagccg atcaggaaaa aacgaaagca gagccgacga agaaaaccaa actttcctac
    10021 aaagaacagc gtgaactgga cgagttaccg gctaaaatgg aacagcttga agccgacatt
    10081 gaagccctgc aagccgaagt aaatagtgcc gaattcttcg ccaaagatcc aagctacacc
    10141 aacgcccagc tacaaaaact tgccgatacc gaagccgcgt tagagcaagc ctttgagcgg
    10201 tgggaagcgt tggaaaatat taaaaacggg aatatctaat gtaggggcga atgattattc
    10261 gcccttgatg gataaaatga ttgggcgaaa aatttttcgc ccctacaaat gtgaagcaaa
    10321 atgacagaaa gattagataa ctacgaacaa cgttttggtg gcattggtcg cctttatacg
    10381 ccggaaggat tggagaaact acgccgttcg catatttgcg tgattgggat tggcggggtt
    10441 ggctcgtggg cagtggaggc attggctcgc tcaggtatcg gtaagatcac gatgatcgat
    10501 atggacgata tttgcgtgac caatatcaac cgccaaattc acgcaatgag tggcacggtg
    10561 gctcaactga aaaccgaagc gatgaaagag cggattgagc ggatcaaccc tgaatgtgtg
    10621 gtggaaatta ttgatgattt cttaacgcag gataatctcg cagactacct caatcgagga
    10681 tatgactatg tgatcgatgc gattgacagt ttgcgggtaa aagcggcgat gattgcgttt
    10741 tgtaaacgca ataagatcaa aattatcacc accggcgggg cgggcgggca gaccgatccg
    10801 accaaaattc aaattaccga tttaagcaaa acggtgcaag atccgttggc ggcaagggtt
    10861 cgtagcctgt tgcgtaaaga gtacggtttc agcaataaaa acaaattcgg cgtggagtgc
    10921 gtttcttcga ctcagcaact gattttccca caaatgggtg aggcctgtga ggtgtcggca
    10981 acaatgaact gtgccaacgg ctttggagcg gtgacaatgg tgacggcaac gttcggcttt
    11041 tttgcggtga gtcgggtgat tgataaacta ttaaaataac aagcggtcat attaaatgaa
    11101 ttttttgcaa atcaaggagt aaataatggc aatacatcga gtcaacccga aagggagtat
    11161 ggaacagctt tcccatttgg aaatggagtt gttggcaaag aatacgcagg gtaatttgca
    11221 tcaactttat cgtaattgct ctttggcggt gctaaattcc ggtgtgcata ccgatgacag
    11281 ccgagcgttg ttaagccaat accctgattt tgaaatccgc ttattaaccc gtgaaaaagg
    11341 cgtgtcgctt gaattgcata atccacccga aactgcgttt gttgacggca agatgatttt
    11401 gaatattcaa tatcaccttt ttgcggtgct gcgggatatt gtgtttgtga atgcgttaaa
    11461 aaatgcgatt agaccgcttg aagagacttc tttagaagcc ttaaccacga ataccgtatt
    11521 ttcgattttg cggaatgcga aagcgattga aatgaatacc gacccaaatt tggtggtgtg
    11581 ctggggaggg cattcgatta atgaaaccga ataccaatat tgccgtgccg tcgggcaaga
    11641 gtttggcttg cgtgagctga atattgtgac cggttgcgga gccggtgtga tggaagcccc
    11701 gatgaaagga gcggctatcg gacacgccaa tcaacgttat aaaaacgggc gttttatcgg
    11761 cattaccgag ccgtctatta ttgcctccga gccgccaaac ccgattgtga atgaattggt
    11821 aattatgccg gatattgaaa aacggttgga agcctttgtg cgtttagggc acgccattgt
    11881 ggttttcccg ggcgggccgg gaacgctgga agagttgctg tatattatcg gtattaagtt
    11941 aaacccggct aaccatgctc agcaattacc actgatttta accgccccga aagaatcggc
    12001 agactatttc tatgccatcg atcaatttat tggcgaaact ttaggcgaag aagctcgcag
    12061 tttgtatgaa attattattg atgactcggt gttggtggct cgtaaagtca agcaagcgat
    12121 gattgaggta aaaagcagcc gttatgaaag tggcgatgct tataatttca actggtcgtt
    12181 aaaaattgag gaagatttta ttacgccatt tattccaacc cacgaaaata tggcaaacct
    12241 gaatttaagt ttagcacagc caaaagagca gctggctgcg aatttaagac gggcattttc
    12301 agggattgtt gcaggtaata ttaagccgga gacctccgat ttaattgaga aatatggggt
    12361 gtttaaatta cacggcgatc gtaaattaat ggagcatata gataaactgt tggagcgttt
    12421 tgtggttcaa caccgtatga aaattccaac gggtgaggcg taccaacctt gctatgaaat
    12481 tgtaaaaggg taatgctaac aaggggtaag gtatgtattt aaagcatatt gaaatcagtg
    12541 gctttcgtgg tattaaccat ttatcgctcc agctacgacc aaatatggta ttgattggcg
    12601 aaaatgcgtg ggggaaatcc agtctgattg atgcattaag cctgattttt aaccttgagc
    12661 aggcgttgta tcaatttcag ttcaaagatt tccatattca atacaccgat acccccaacc
    12721 aagtgcggga cattcaactg gtgtttacct tttgtgaaga gcatcagcac gatttagcgg
    12781 tgcaatttcc cgcttatttg gcacaagccg cgttttatat tgataatcac cgctgtgttt
    12841 cgcttgccgt aaccggaaaa ttacacggag agcagattag caccgactat caatttgtgg
    12901 atcaaagcct aaaaccgatc cagatagaac aagcggaaaa cgccattcgg cagtttattt
    12961 accatcaccc tgtttatcgc ctgcgtgatg caaggctcaa taacagggtg cataatgaat
    13021 tattacttaa aggatcgtct caacttgaag acgattttca acgtgaatta aacgccctct
    13081 cttcgctact gaaatactat tttctaacca gccaaagtcg ctatttgatc aaaaccagta
    13141 tgcaagacac cgcattgtgg tgggaaaaag taaaatccct ctgtttacgc ctaaaacagc
    13201 agccggaatt ggtcgaatct gctcagcagc atatttctac tctatttttg tttaatccta
    13261 gatctacagt aatagaaaag ccgattattt tatttgaaga tctgaatgct caactacacc
    13321 ctcgaatgat tgcgattttt tgggaactgc tcggtttttt acccagccaa cggatcacca
    13381 ctactaattc tattgaattg ttatcgcaag tgcctctgcg tgaaatctgc cgtttggtgc
    13441 gtcatcacga tcatacccaa gcctattggc ttgaacgcca cagtttaggc aaggaagatt
    13501 tgcgaaaatt aacctttcat attcaccata atcgtggctt ggcactgttt gctaaagcgt
    13561 ggattttggt tgagggggaa acggaggttt ggattttgca agagctggcg agattattag
    13621 atattcattt ggaaatggaa gggatcaaaa tcgttgaatt tgctcagtgt ggcttacgtc
    13681 cgttaattaa atacgccaga gcaatgggga tcgaatggta tgtgctgact gacggtgacg
    13741 attccggcaa acgttatgca gatacggtaa aaagtatgga agaggatttt gctcaccggc
    13801 tcacgatgtt gccacaaaaa gatatcgaac attttttcta caaagcaggc ttgcggaatg
    13861 tgtttataca gcttgcaaat tggcgttcac aagaaaaata tccggtgtcc tacatcatta
    13921 aacgagcgat tcaacgaagc tcaaagcctg atttagccat tgcattagcc aatgagatgg
    13981 ggcaacgggg agagcattca attccaactt tatttaaaca attatttgca gatgtgctgg
    14041 cattgataaa caaagtatag aatacaagcg gttaaatttg acaaaaaatt gccaattttg
    14101 accgcttgtt tgcttgtgat gaaaaccaat catacaaaac tctaatttat ttcaagtgca
    14161 agctattatt ttatgcgtta tgcttatttg gaataaaaaa atcgggttag ttgattgaca
    14221 tagcattcta gacgtctaaa ataaccaaaa ataatagtta attgaatgtg gataaataga
    14281 aaatgattca gctgaaaaat atcagcaaac aatttgaggt aaagggcaaa aaaattaccg
    14341 ctctggataa tgtaaaccta gaagtaccaa gaggcactat ttatggtgtc attggggcat
    14401 caggggcagg aaaaagtacc ttaattcgct gtgtaaattt attagaacgc ccaacaatcg
    14461 ggcaagtgat tgtcgatggg caagatttaa cggcaatgtc ggataaagag ttgattcacg
    14521 ctcgccgtaa tattgcaatg attttccaac attttaattt gctttcatca caaactgtat
    14581 ttgaaaacgt ggcgttatcg ttgcgtttaa gcaatacgcc aaaagagcaa gttgagaaga
    14641 aagtcaatga gctgttggat ttagtggggt taacagatcg taaagatgtt taccctgcaa
    14701 atctttccgg tgggcagaaa cagcgtgtag cgattgctcg tgcgttagcc agcgatccac
    14761 acgttttgtt gtgtgatgaa gcgaccagtg cgttagatcc ggcgactacg caatccattt
    14821 tgcagctatt aaaagatatt aataaacgtc ttggtttgac gattttatta atcactcacg
    14881 aaatggaagt ggtaaaacgc atttgtgacc gagtagcagt gattgataaa gggcagttga
    14941 ttgaatctgg ctcggtaagt gaaattttct cgaatccgaa aaccgaatta gctcagaaat
    15001 ttattcaatc gactttccat tttgaattac cgcaagaata taccgagcag ctttcaagtt
    15061 cgccgactga acatagtaag ccgattatta aatttgagtt taccggacgt tcggtggatg
    15121 cacctttgct ttcaatggca tcgaagaaat tcggcattga ttttagtatt ttgatgtcgc
    15181 aaattgatta tgcaggcggg gtcaaatttg gatttgtaat tgcagaagtg agtggtaatc
    15241 cggattccat tgcagaggca aaattttatt taattgataa caatgtaaaa gtagaggtgt
    15301 taggctatgt gggctgattt tatgcaggag atgacgccac agatgtggag cttagtatgg
    15361 gaatctactt ttgaaaccgt ttatatgggc tttgtggcaa cggcattggc ggtagtcatc
    15421 ggtttaccga ttggtttctt ggcgttttta accggtaaag gcgaaatttt agaaaatccg
    15481 agattacacc aagtgttaga tgtcgttatt aacatcgggc gttcgattcc gttcattatt
    15541 ttattgatta ttttgttgcc atttactcgc ttagttgtgg gcacaacatt aggcacaacg
    15601 gcagcgattg ttcctttaag tgtttcggcc attccgtttt ttgcccgttt aacttcaaat
    15661 gcgttgcttg aaattccgtc cggtttaaca gaagcggcaa aatcaatggg agcaacaaat
    15721 tggcaggtag tgacaaaatt ttatttaccg gagtcattac cgattttgat taatggcatt
    15781 acgctaacgc tggttgcttt aattggctac tcagcaatgg cgggggctgt tggtggcggc
    15841 ggtttaggca atttagcgat tagctatggc gaacaccgta atatggtgta tgtgaaatgg
    15901 attgctacca ttattattgt tgcgattgtg atgattagcc aaaaattggg tgatcattta
    15961 gcaaaacgtt acgatcatcg ttaattttat ttaaaaagag gtttctatga gtttcaagaa
    16021 aattttaggc gttgcattgg tttctgcatt agcattaacc gcttgtaaag aagagaaaaa
    16081 ggcagaatct accgctgctc cggcagctca agctccggct aaaatcaaag taggggtaat
    16141 gtcaggccct gagcataccg ttgcagaacg tgcagcacag attgcaaaag aaaaatatgg
    16201 tttagaagtt gagttcgttt tatttaacga ctacgcttta ccgaatactg cagtaagtaa
    16261 aggtgattta gatgcaaacg cattccaaca caaaccatat ttagataaag acagccaatc
    16321 aaaaggttta aacaacttag tgattgtggg taacaccttt gtttatccgt tagcgggcta
    16381 ctctaaaaaa gtgaaaaatg tgtctgagtt agcagaaggt gcggtaattg cagtaccaaa
    16441 tgatccttca aacttagctc gtgctttgat tttattagaa aaacaggggt taattaaatt
    16501 aaaagataat accaatttat tctcaacttc agtggatatt atcgaaaatc cgaaaaactt
    16561 aaaaattaaa gaagtggata cttcaattgc cgcaaaagcg ttagatgacg tagatttagc
    16621 ggtggtaaat aacacttatg ccgggcaggt aggcttaaat acccaagatc acggtgtatt
    16681 tgttgagtct aaagattcac cgtatgtgaa tattattgtg gctcgccaag acaataaaga
    16741 tgcagctaat gtacaaaact ttattaaatc ttaccaaacc gaagaagtgt accaagaagc
    16801 acaaaaacac tttaaagatg gtgtagtaaa aggttggtaa tttagctgat ttttacttaa
    16861 atggcgatta agcgctaggt taatcgccat tttcatcttt aaaatatagg aaggagcaaa
    16921 caatgaactt taaaaaatta ttaggtgtgg cgttagtatc tgccttagca cttactgcgt
    16981 gtaaagatga aaaagcacaa gcacctgcta caacagctaa aactgaaaac aaagccccat
    17041 taaaagtggg tgtgatgacc ggccctgaag cccaaatgac cgaagtggca gtgaaaattg
    17101 caaaagagaa atatggctta gatgtagagt tagtgcagtt tactgaatac actcaaccaa
    17161 atgccgcact tcattctaaa gatttagatg ctaacgcgtt ccaaacggtg ccttatttag
    17221 agcaagaagt gaaagatcgt ggttataaat tagcgattat cggcaatacg ctagtatggc
    17281 caatcgcggc ttattctaaa aaaattaaaa acatttccga gttaaaagac ggagcgactg
    17341 ttgcgattcc aaacaatgca agtaatactg ctcgtgcgtt attattgctt caagctcacg
    17401 gtttattgaa attaaaagat ccgaaaaatg tgtttgctac cgaaaacgat attatcgaaa
    17461 acccgaaaaa tatcaaaatc gtacaggcgg atacctcact tttaacccgt atgttagatg
    17521 atgtagaact tgcggtaatc aacaacactt acgcaggtca agctgggtta agtccggata
    17581 aagacggtat tattgtggaa tctaaagatt caccgtatgt gaatttagtg gtaagtcgtg
    17641 aagataataa agatgaccca cgcttacaaa cttttgtgaa gtcattccaa accgaagaag
    17701 tattccaaga agcgttaaaa ttatttaacg gtggtgtggt gaaaggttgg taatcagttg
    17761 gacggcatat tctgccgtcc ttttcttaaa aggagaaaca gataatgaaa ataatgaaat
    17821 tagccggtgc agttgcaatt ttctcgcttt ttttaaccgc ttgtaatgat aaagccgaaa
    17881 agttgaaagt cggtgtgatt tccggccctg aacataaggt aatggaagtg gcggcaaaaa
    17941 ttgcaaagga aaaatataac cgtgatgttg aattagtggt atttaccgat tatgccacgc
    18001 ctaatgcagc tttagataaa ggcgatcttg atttgaatgc tttccagcat aaaccttatt
    18061 tagataacca aattcaggaa aaaggctata aattagtgcc ggtcggcaat acttttgttt
    18121 atccgattgc ggcttattcc aaaaaaatta aatcgctggc agagttgaaa gatggtgata
    18181 ctattgcagt gcctaatgat ccgactaatt tagcccgtgc tttaatttta ttggaaaaac
    18241 aagatttaat taagctgcga gcagatgcag gcttaaaagc aaccagtgtg gatattattg
    18301 aaaaccctcg taaattggtg atccaagaaa ttgaagcacc attattgcct cgaacgttgg
    18361 acgatgttgc cttttcgatt attaatacta cctacgcagg gcaaaacggt ttaacgccaa
    18421 ccaaagacgg aatattcgtt gaagacaagg attcgcctta tgtgaattta attgttgccc
    18481 gtgaaaataa ccaacattct gaagccgtaa aagatttggt gaaagcctac caaacagaag
    18541 aggtgtataa caaagcgaat gaagagttta aaggggcgat gataaaaggc tggtaattga
    18601 agataaataa ggcatagatt cctatgcctt actttttatg ttacagacct gctccggctc
    18661 ttaatgcttc ggctttgtca gttttttccc aaggaaaatg tttacgaccg aagtgtccgt
    18721 aggcagcggt ttcacggtag attggtttga ttaaatctaa cattttgatt aagccatacg
    18781 ggcgtaagtc aaagttttgg tagattaatt tcacaagtat ttcgttgctc actttacctg
    18841 tgccgaaggt ttcgaccata attgatgttg ggtcagccac cccgatagca taagaaagtt
    18901 ggatctcaca gcgatcggct aagcctgcgg caacgatatt ttttgcgaca taacgagcag
    18961 cataagcagc agagcgatcc acttttgacg ggtctttacc agagaatgca ccaccaccgt
    19021 ggcgagccgc accaccgtag gtatctacaa tgattttacg cccggttaaa ccacagtcac
    19081 ccattggacc gccaatcaca aaacgaccgg ttgggttgat gaaatatttg gtgttttggc
    19141 tgagccattc gcttggtagt accggtttga tgatctcttc catcacccct tcaattaact
    19201 ctttttgcga aatatgctct gcgtgttgtg tagaaagcac tacggcatca atgccgataa
    19261 ttttattgtt ctcataagca aatgttaatt ggcttttagc gtcagggcgt aaccaatcga
    19321 gtttgcccga tttacgcact tttgactgct gttccattaa gcggtgtgcg taagtaattg
    19381 gagccggcat taacacctcg gtttcattgg tagcgtagcc aaacataatc ccttggtcgc
    19441 ccgcaccttg ctctaacgga tcggcacggt caacgccctg gttgatgtcc ggagattgtt
    19501 taccaatcgc atttaatacc gcacaagagt gagcatcaaa gcccatatca gagtgagtat
    19561 agccgatgtc gcaaatcact tggcgggtta agttttcaat atccacccac gcagaggtag
    19621 tgatttctcc ccctactaat gccataccgg ttttcacata agtttcacaa gcaacgcgtg
    19681 ctttagggtc ttgttttaaa atttcatcta ataccgcgtc tgaaatttgg tctgcgattt
    19741 tatccggatg cccttctgaa accgattcag aagtaaataa attaattgcc atttgtattc
    19801 ctctctatct taaatatgga ttattaatag tagggacggg tccagtgccc gcccataaat
    19861 tatataaaat gtttggacag gcacaggacc ggtccctata aaatttgttt ggaagtgttt
    19921 ccgtctagac gtctataata tattttttga acgttttagg caaatagaat ttgtttattt
    19981 ggctaaatat gcccttaaac cagccaacgc atttttggca tcttctaccc ctaaatcgta
    20041 cattgcttgt attttattta catccgtttc aaggcgactg attttgactg ttctgcttgg
    20101 acgaatcaca aaaatctgtt gtttttcatt gagttgaata atctgctcaa cggcttgatt
    20161 atattccgca tagcggtttt cccagcgttg cactaatttc ggatattttt tataaaaacg
    20221 tttaaacagc cacatcgagc ttggtttctt acggtattca agtgggcgag taagcaccac
    20281 gataattttg tcatatccca tttcaataaa tttctgcacc ggaatactgt ctgacacacc
    20341 accgtctaaa tattttttac cctcaatttc gactattttg gatacaatcg gcattgagga
    20401 agtagctcga aaggcttcca tttgctcgaa cacattgtca attttgatgt attcgggctc
    20461 accggtttcg acattggtta cagtaaccca aaagtccata ccggaatcct caaaggcttg
    20521 ttggtcaaac ggatccagtt tggaagggag ttcatagtaa gcaaaatccc gattaacgat
    20581 attgccggtg gtaagcaagc tgtgtaaccc catataccgc ttatctttag cgtatttttt
    20641 gttgtatcgc aacgccctac ctttttgttt tgaaggcaaa ttcacgccaa ataacgcacc
    20701 ggctgaaaca ccgattgctc catctatatg aatatgttca tctaaaaata catctaacac
    20761 gccggcagta aaaatacctc gcaaaccacc gccttccagc actaatccaa ctttcatatc
    20821 attctccctg taatttgtgt attataaccc gaattatctt gctaggagaa atcacaatgg
    20881 ctgatgcaga atttatggat ttcctgttag atcaactaag cccgatttcc ggtttgcgtt
    20941 cgaaaaaaat gtttggtgaa tattgcttgt tttttgataa taaaattgtc gctattatca
    21001 gcaaagatta tcgcattttt gtcaaagcta atccgcaaac cttgccgtta ttcttagctg
    21061 aaaatgccga acaatttcac tattttgcta aaggaaaagt gcaaaaaatg cactactgga
    21121 gcattccgga attagcgatt gaagaagccg atgaactggt caaatggatt cggctaggct
    21181 tacaagcggt gtaatttagc gaaaattttg taaaaaaacg ctaaaatatg cccgcttgtt
    21241 aggggggggg acctaatatt tctatacttt aagattttat atttatgaac caaaaaaaat
    21301 tagtttcttt gattgtcgtt attgtgttgg caatcatttc tcaaattttt agcttttttg
    21361 ataaaaaaga gccttcaaaa gcacaagaaa aatcgaccca gagtagtcag tctcatcaag
    21421 tttcaacttc atcactaggg aactatgatg tgcagatgaa aaacgaccgt ttgaaacagc
    21481 atattgtaga taccgattat tacaccttag cgttgtcgtg gtcgccggcg ttttgccaaa
    21541 aacaaaagcg aaataatcac ggtaaaattc cgtctcattt ggaatatcaa tgtggtggct
    21601 cagcggattt tggttgggtt attcacggct tatggccgca aagtggcaag gcaagaagtg
    21661 ttgatgaaca ccctcgttat tgccaaggcg atttgccgat ggtggcagag cagacgatta
    21721 agcaatattt acctgaatcg ccgggggcaa ctttgttgca aggcgaatgg gaaaaacacg
    21781 gtgcttgtgc ctttaaccaa gcggatgatt acttcgccaa acaaaaagag ctatttaatc
    21841 agctcaaatt accggatacc gaaatgtcta aattagcact gtttaactgg atgcgagcca
    21901 ataaccctca tttgcaaaat gtctatttag ggggtagcaa agatgagctg tatatttgct
    21961 atgataaagc atttcaacca atggattgcc cacgctaatg gcagatcttc gccaactgaa
    22021 aatgcaggtt cagcgccaat taaagcgtga tcttgaacgt gcaaatcact attttaatac
    22081 cacttttacg ccaccaagta ttagctatgc ggtgcgtggc gtaaaagccg gtgtggctta
    22141 tcttgaacgc aacgaagtac ggtttaatcc tgtgttattg caagaaaatg aacaggcatt
    22201 tatccgccaa gttgtgccgc acgaactggc tcatatcttg gtttatcaac attttgggcg
    22261 ggtacaatct catggtaaag agtggaaaat gatgatggaa accgtactgg gtgtgccggc
    22321 ggaaatttac cattgttttg atacccaaag tgtgcaacag caatttagtt accaatgtgc
    22381 ctgccagact caccagcttt cgattcgccg acacaatgcc gtgatgcgag ataaaaaaag
    22441 ctatatttgc cgtttatgta aaactcaact ccattttgta gaaaaaagtt aatgtggaga
    22501 aaagttaaga gaatgttacg atttagctag acattcctgt ttaaacgcct acaatttgcc
    22561 gtagttttta tttttaattt tatttccgga gtatttatgg aaacaattgt aattgtaggt
    22621 ggcggtgcag gcggtttgga gcttgcaacc tatctaggca acaaattagg gcgtaaacaa
    22681 aaggcaaaag tggtgctgat tgaccgcaat gcaacgcatt tatggaaacc actgttgcac
    22741 gaggtggcaa ccggtactat tgatgaggga actgatgcgt taagctatcg ggctcacgct
    22801 aaaaatcata gttttgaatt tcagcaaggt acgctggtgg cggtagatag agagaagaaa
    22861 acggtttcac tcgccccgat ttataatgat caaaaccaac ttctcgttaa agaacgccat
    22921 atcagttatg acaaattagt gatcgcgatc ggcagtaaat ccaatgattt tggcaccaaa
    22981 ggggttgcag aaaattgcat tttcttagat ggctcagagc aagcaaaaga tttccaaaaa
    23041 cgaatgatgg agctgttttt acgtttttca catagtgaaa ataaagatgt aaaaattgcg
    23101 attgtggggg ggggagcaac aggtattgag ctttcagcgg agctttacca cgtggtgaaa
    23161 aatttaaatt cttacggctt tggtaaatta aaccgtgcca gcttgaaagt cactttaatt
    23221 gaggcaggcc ctcgtttgat tccggcatta ccggagaaag tgtcggtttc tgctttccac
    23281 gaattgaaaa aagcgggtgt ttcggtgcgt ttaggtacga tgattaccga agctcgtcca
    23341 gatgggctaa tgacccgttt aggtgagaaa attgaggcgg atattatggt ttgggcggct
    23401 ggcgtgaaag cccctgaaat tacccgtgaa tttggttttg aaactaaccg cttgaaccaa
    23461 attgagatta aagacacgct acaaaccacc gttgatgagt cagtgtttgt cattggcgat
    23521 tgtgcatcat taattcaaga tggcaagcca attccaccca gagcacaagc agcccaccaa
    23581 atggcaaccc gatgtggtaa aaatattgtg gcattgatag aaggaaaaga gatgaagccg
    23641 tttcgattta acgataaagg ctcactgctt tccttctcaa aattcggcac ggtgggtaat
    23701 ttaatgggga atttagtgag cggtgatatg tttattgaag gtaaaattgc ccgcttggct
    23761 tacctctcgc tttatcgaat gcaccaagtg gcattacacg gctgtgtcaa aaccggcttg
    23821 attgtgttgg tagggcaaat taaccgtttc ttaagaccaa caatgaaact acattaaaca
    23881 aaaatccgca tcaaatgatg cggatttttt atggcaaaat aacaagcggt tattttcttg
    23941 cctatttttg caaatactca cgcctagttt gctaatttaa tttccgccaa ccatttatca
    24001 attaagcgtt tgccctcttc gctggaacct gctttttggt aatcaatttc cacaaagtct
    24061 aacgcttcgg ctttggttga ttgtggagca acttcggcgt tgatgtttgt tggggtttgg
    24121 taaacaccgt gttttttcca aggaatttcc tgtgcttctt ttgaaagcac ccaatccata
    24181 aagagtttgg cgttatctaa attacgggca tttttgataa tgctgacacc gcctaacgca
    24241 tagcccactt taccttccgg aataatggct tcaataggcg cacctttttc tttttcagtg
    24301 gcataactat gtacaaagcc taccgtaacc gcactttcac cacgagcaag attgctggtg
    24361 actagggtgc ttttcacata ttgcgaaacg ttattgttga gttttttcag ataatcaaag
    24421 gttttctctt cgccccataa ggttgaaaga gtggtcataa acgtataagt cgtgccggag
    24481 ctacgaggat caggcagttg tacttcacct tttaagcgag gatctgataa atcttcccat
    24541 tttttcggtg cctcaatgcc tagtttggcg agtttttcgg tattcacgcc taaccctaat
    24601 acaagcatat aaacgataga ggtaaattta ccttgttcac tttcaactaa ggatttaaat
    24661 tgtggcagaa tttcagcttg ttttggtgaa cggtaggctt ccagtaagcc taattcgcct
    24721 gcttgaaagt gaggttcaat cgtgccgcca taccaaatat ctgcctgagg gttcgatgct
    24781 tccgctttta cttttccgaa aattgtaccg gttccgccgt gaatgaattt agtttcgaca
    24841 ttatatttgt gtgaaaattg cttggtcatt tcttcacaaa cattattttg taccgagcaa
    24901 taaacagtta aacgaccttc tgcttgtgct gcttgagctg ataagaaaat gccagaggcg
    24961 attaagatcg attttaatga gattaatttg agtacgttca tcactttcct gaaaacataa
    25021 aataaaaggt agtgaagtct agcatttcaa agcgctggtt gaaaatagtt aattgcaatg
    25081 aagtagggca attgtgccta ttactttaat atttaccaac tatttgcaag ttttagcaaa
    25141 aaataaccgc ttgtagtagc tcttgctatt ttgtcaaaat tcagaataat aaatgccttt
    25201 agtctaatgt taaattttag ttatggaaaa tagttggaaa tatgctttta aattaatcac
    25261 gccgattttt atgggctatt ttgcggcagg ggttgcttac ggaatgcttg ccaccaatgc
    25321 cggaatgcct gcgtggttta cgattgtaat gagttttacc gtggtatcgg ggacggcaca
    25381 atatgcagcg attccatttt ttgtctccgg tgttggggcg gtgtcggtct ttctcagtac
    25441 gcttttaatg agtctacgtt ttagttttta caccttaaat atgatggagc ataagccgat
    25501 atccaaagcg aaaagtctgg tcgctattgc aggtttaact gatgaaggct ttgcggtgtt
    25561 atcttcatta ccgaaagagc aaaagcaagc attattcttt aaaatcgctg tgctatgtgt
    25621 ggcttattgg acattttcca cgattgtcgg tgtgttgtta ggcgattcag tggcgaacta
    25681 tattccgcat ttggattttg ctctgccttg tttgtttgca attttagcct atgagcaata
    25741 caaaaaccaa aaacaatgga aaccgattgt gattgcgtta atcggatttt tacttgctcg
    25801 tcagattacc gaaaccagcg tgttattggt ggcaatttta gtagcgattg tcattgtggc
    25861 tttattacct caatcttctt caatcaaact agagaaagga gtagaaaatg agcacaaatg
    25921 atactttgat aatgtttgca gcattagtgc tcggctcgca aatttgtcgt tttttgccgg
    25981 aatttttacc gaaaaaggtg ttatcttcgc ccattttgca aaaattaaac aaaatgttac
    26041 cgcttgtgat tatgctttta ttgcttttaa cgagcctaaa ctttcctaaa aacggggaag
    26101 gctttaactt aatattggca caaattctgg ctttaatagg cgtattaggt agctatgttt
    26161 ggcttaaaaa taccttttta agcgtagcgt tggggatttt cggtgtgaat gtatttttat
    26221 ccctgttagg ataaatacca aaggtcattt tataggagcg aaacaccttc gctcaatcat
    26281 taacagatgt ggggcgaaaa atttttcgcc ccttatttat cacaagcggt ctttttcttt
    26341 caattttttg caaattaaat cgctaaatca aacgcataga aggcaactaa aataatcgca
    26401 ataattactg tacctaggtt taatttattg gcttcgcctg aaattaagcg accaatcaca
    26461 agggtggcga aacctagcat aatgcctgtc acaatgttgg cggttaatac gatgaacacc
    26521 gcacagacta aacctgacat tgcacctaca aaatcgctga aatctaattt agagacatta
    26581 cttagcatta ataagcccac atacattaat gccggtgcgg tggcatagct tggtactaag
    26641 aatgcaagag gttggaagaa cagcataaat aagaaaccga taccaaccac tacagcggtt
    26701 aaacctgttt taccgccaac ggctgtacct gctgccgatt caatatacac tgccgctggt
    26761 gctgtaccaa ataagcccga taaaattgat ccgaatgagt cagaagttag ggctttatcg
    26821 ccattgataa tttgtccgtc tttatctaat aaaccggctt gccctgcaac cgcacggata
    26881 gtacctgttg catcaaaaat agcggtcatt actaacgcaa acactaccgg taaaatggcg
    26941 gtatttaatg ctcccataat atcaagattt aggaattgcg aattttcacc aaaactcggc
    27001 attttgaaaa agcctgtgta ttttacgtta gggtcaaaaa ttaaacctac gatggtaata
    27061 gcaataatga cccataaaat actgcctttt actttcagct tttctaaccc gataatggcg
    27121 gctagaccga ttaatgacac taaaaccgga aatgaagtga agtcccctaa tttaaccgga
    27181 atatccatac cgctattgac cactaattta acgttagtgg cggcaataat cagtaagaat
    27241 aaaccaatac caattccagc accgtgggca atgctgcttg gcaaattacg taaaatccaa
    27301 gtacgaatac cggtagcaga aatcaaggta aatactacgc ccattaagaa aatcgccccg
    27361 agtgcaacag gaatagaaac ttgttgccct aatactaagc taaaggcagt aaaagcagta
    27421 agtgaaatcg cacaaccgat tgccattggg gcatttgccc ataaaccgat taagatagag
    27481 ccgagacctg aaactaaaca ggtggcaata aataccgatt cggcaggaaa gcctgcttgg
    27541 cttagcattc ccggcacaac gatgacggaa taaaccatgg ctaaaaaggt agttaaaccg
    27601 gcgatgattt cttgacgagc gtttgaacca cgctcctcaa atttaaataa cgacataaga
    27661 acctcaaaag aagaaaatga aaggggaaat tttaggatgc ggaattttac agaaatttaa
    27721 aataattagc aaacgtttgc gtaaaaagtg aaagaaaagc aggaataaat cctgcttttg
    27781 tttgatggtt agtaatcgat gaaagcgtct tgatctgccc cttccttttc aactttaggc
    27841 tgaaccatat gttcacgttt tagcccaagt tgtagtgcca caccaattgc aatgtagatg
    27901 gatgaataag taccgaaacc gataccgatc aataatgcaa gtgagaaact atgaagcgtt
    27961 ggaccaccta gatagaataa ggctagtacc acaaagaggg tggttactga tgtcattaaa
    28021 gtacgcgaaa gtgtttggct caaggaaata tcgataattt caattgagct ggagcgacga
    28081 actttagaaa agttttcacg tacacggtca aacaccacaa ttgagtcatt taacgagtaa
    28141 ccgacaacag ataaaatcgc tgctacaaag gtcaagtcaa tttcaatctg taagaaagag
    28201 aagattccga tcgtcactaa tacatcgtga aataaagcca caataccgcc taccgctaaa
    28261 cgccactcaa aacgtgttcc cacgtaaata agtagcatag caagtgttgc caaagtggca
    28321 taaatagccc cttgggttag ctcttccccc acgtttgggc caacgaattc aatgctcttg
    28381 atagtcgcat tcggatctaa tttttgatgg atcatctcca tcactttatt cccaacagac
    28441 atatcaccgg cactggcggc aagtcggatc attaaatctt tttgactgcc tgtggtttgc
    28501 actaaggcat tgccgtagcc gctattttca agctccgtac gcagttttgc taaatcagca
    28561 ggttgtgaaa attgagtttc gattactgtc ccgccggtaa aatctaaccc ccagttaaag
    28621 ccttttgtcg caatagtaaa aatgcataaa gcagttacaa tcagcgaaaa aatgaatccg
    28681 acatagcggt atttcataaa ggggattaat ttataaggca gtttaatttc tgctacatct
    28741 tgttgtacat tttcaatagc catttttcca ccttaaatcc aaagtttctt cacgcgtttg
    28801 ccaccgtaaa cccagtttac aagcattcga gtacctgtaa ttgcggtaaa catagaaata
    28861 attaccccta atgcaagcgt taccgcaaag ccttttaccg gacctgtacc tacaccataa
    28921 agcacaagag aagtcaaaat agtggtgagg ttggagtcaa aaatacttga gaatgcgcca
    28981 ttataacctt cgtggattgc ttgttgaatt gggcgaccgt tacgaatctc ttctttgatc
    29041 cgctcgtaga ttagtacgtt ggcatcaact gacataccca cagcaagtac gataccggca
    29101 attcccggca tagtaagggt tgcaccgatt aatgacatta aaccaatcgt taagatcata
    29161 ttcacaatca aggcaagaga ggcaaatata ccaaatactt tataaaaggc tagacagaac
    29221 aggatcgtta agcctaagcc taaaatacta gcttccatac cttgtttaac attatctgcc
    29281 ccaagagaag caccgatagt acgctcttcc acaatcacga ttggagcaat caacgcacct
    29341 gaacgtaata ataccgcaag gttttgagct tccgctggtg aattaatccc tgtaatctgg
    29401 aattgattac ctaaacgggt ttgaatggtt gcaacgttaa ttacctcttg gtgtttttct
    29461 aaaatgactt taccgttagc atctttacga cctgaatctt taaattcggt ataaagtgtt
    29521 gccatcggtt tttggattgc cattttagta atttcaccca tcatctcgcc gccttcggta
    29581 tcgagtcgga tactcacttg cggcaagcca cgctcatcag tgcctgaggt tgcatcaata
    29641 atatgttcac cccctaacga aggtttacgg cgtaatacaa ctggctcgcc atcacgggtg
    29701 ttttgcagtc ctgaatcagc cggcacaatg cctttcgccg cagaggccac attggcttcg
    29761 gtattgacta accggaactc aagggttgca gttgccccta aaagctcttt ggcacgagcg
    29821 gtatcttgca cacccggtaa ttcaactacg atacgatctg caccttgacg ctgaatggtc
    29881 ggctcagaaa cgcccaattc ctcaacacgt ttacgcaaaa tagaaaggtt ttgttcgatt
    29941 gccatcgtgc gagattcaga aagagcttgt gctgatggcg ttaaggtgac tttattatca
    30001 tttaccaaaa tatcaagcgt tgggtgagag agacgcacta aacgcactgc tttttcggcg
    30061 gtatcgttat cggcaaattc aacctcagtt gcaaaatttt cgcctttttt gaccgcttga
    30121 tattgcaatt tttctttacg gaaatcacta cgcaggctgt cttgcaaagt gtcttgttgt
    30181 tttgcaagcg ccgtgttcat atccacttcc attaagaaac gaacaccgcc aagcaagtcc
    30241 aagcctcgct tcatcgggct accgccaatg tcagacaacc actgtggcgt tgctggtgca
    30301 aggtttaagg caacagagta gttattcccc aatgcttctg agattttttc tttggcaggc
    30361 aattgctgtt cgctgctttc gagacgaacc aaaatcgagc cattttctaa cacggcagat
    30421 ttcggctgaa tattcattgc tgaaagtgtg gattgcactt gagacagtgt ctcaggtgtg
    30481 gcttgttgcc cacgagtacc ggaaatttgt accgaaggat catcgccata taaatttgga
    30541 agggcatata aagcaccaat ggcgatcacg aagatcacca ttaaattctt ccatagaggg
    30601 aaacggttta acacaataac tccccgaatg ttaaagttgg ataagagggt tgattaaagt
    30661 gattttaatg aaccttttgg taacaccgca acaacgaaat cacgtttgat ggtcacttca
    30721 gttgtgtcat ttaatgcaat cacgatgtta tcgttatcag cggtcacttt ggtaatttta
    30781 ccgattaaac caccgctggt taatacttca tcgccttttg tcaagcttgc cagtaaatca
    30841 cgttgttgtt tttggcgttt tgcctgagga cgataaatca taaaatagaa aattgcaccg
    30901 aaaataatca ggataaagat catttccatt ccgccacctt gttgcataaa aactccttaa
    30961 atgtttgagt ggtataaaaa taagtgttat aaaaaacgcc tgttaaaata ccataaattc
    31021 aatgaattaa cgagtagaaa aattcatcga atgcttttct tttaaacaaa aatttgagta
    31081 gaatcagcac ttttagttta ggattaggtt cttggcgtgt tcttgcgctg acacaatagg
    31141 agaaaaaatg aaaaaattat tcgctcaagc ggttattttt ggggcttttt ttgcaagttc
    31201 cgcttttgct cacaatgtgc aattaaatca agcattacct gctgtttcgg ttgcaaaaga
    31261 cggggaattg gcagtggcag gtggaaaggt tagctataaa aattggcatt catcatctct
    31321 ggcaggtaaa gtccgtgtta ttcatcattt tgccggtaga agttcggtga aagaaaaaaa
    31381 tgaagatttg atgacagcaa ttaaaaatgc cggttttgat cgctcgaaat accaaacggt
    31441 gacgattgta aatgctgatg atgcgattgt cggcacaggg gtatttgtga aaaatagtgt
    31501 ggaaaacggc aaacttgaca atcctcacag ccaagtagtg ttagaccagc aaggcagtgt
    31561 gaaaaaagcg tggggcttaa aagagaaaga gagctttatt gcagtgctgg ataaatcggg
    31621 taaggtacaa tttacctctg agggcaaact ctctgcaaaa cagattcaag aagtgattgg
    31681 gttggtgaaa acattaacgc aataaccagt gtttttatgg gcggattttg tatccgccca
    31741 tcattctaga taataaatcg ataacttatc gtgtgatgat actcttctcc tgcttttgtt
    31801 atcccgccaa attggagtaa ttcaggctgg tttagtgaat ttgggaaaaa ctcaggctct
    31861 aatgctacac cagcgtaatc ttcatattcg cctttagtca aattaggttg tccttttagc
    31921 cagttgccgg tgtaaagttg taatgctggc atggaggtgc ggagttccat ttttaagtct
    31981 tccacggcta aacaagcggt cggattttca ctattttttg caagatgaaa ggagtgatcg
    32041 taacctttta ccgctttttg gtcgtcatca gccaataaat cttgcccaat ttttttagca
    32101 ttgcggaagt caaaactggt actctccact tttttcagtg gagcattagg aataccgctg
    32161 gcatcaaccg gtaagtatcc gtctgcatta attatcagct cgtgatctaa aatcgtctct
    32221 gaaccttgca aattaaaata ggcgtgatta gttaagcaaa gcggtgtatc ttggttagca
    32281 acggcatcaa agctgatttg aagctcattc tctgttaagc gatactccac aaaagctttc
    32341 acttcgccgc caaatccttc ctcgccatct gcaaaaaaat aactaaattt gaccgcttgt
    32401 tcgctttggc tttcaagttg ccacactatt ttatctgccc caagctcgcc accgtggaga
    32461 ttatgttcac cgttattttt agcgagctgg taggttttgc cgtttaagcg atattcggca
    32521 ttggcaatgc ggttagcgta acgcccaatg gtcgctccaa agaaagcggt ctgtttttgc
    32581 caattttttg cagacgtggt gactaatact tcccgttcct cgttcggcat tttgacaatg
    32641 cacgaaagcc aagaagcccc aacatcagaa agggtaatgc gtaaaaacgc attttcaagg
    32701 gtgaataatt tcatcggttg accttaatta cctagcacgg catagccgta ctaggttaag
    32761 cataattcag ccctgctagg gctgaggtta tttattttag agtagcggag ctacttcctt
    32821 atgcttaacc cggtacgctt tagcgtgccg ggcattctcg caccccatcg gaggcagtac
    32881 aaacgtggaa ggtttcttta atgccggttt gcttttcgta attgttagca acggcttggc
    32941 ggagatcttc cactttgtca tttggcacta ggcacacgac acaaccgcca aagccgccgc
    33001 cagtcattct ggctccgccg tttttaccga tagcttgttg ggcgatttcg gctaagcaat
    33061 ccacttcagg gcaggtgatt tcaaaatcat cacgcattga gtcgtgagat tgagccatca
    33121 actccccgag tttgaccata tcattcgctt ttaaggcctc tactgcttcc agaactcgtt
    33181 gattttcgct gatgatatgt tttgctcgtt tatacgccag ctcattctct tcactaagtt
    33241 ctgaggctcg ttcaataaat tgttccgctg tgacatcacg caattttgct ttgccgaaga
    33301 agtgggcggc aaattcgcac tgttcacggc gactgttgta ttcgccggta accaagtcgt
    33361 gttttacgtt ggagttaata atcgcaatcg aatagccttg tggcacaggg gttggggtga
    33421 tttctaaact acggcagtcg atcatcacta aatgatcttg ttgcccgaga gcggaggtga
    33481 gctgatccat attgccgcag ttgcagccga caaatttatt ctcagcctct tgcccgatta
    33541 atgcgatttg cggtaaagaa agcggtaaat cgccgagtaa ttgagcggtt ttaccaatcg
    33601 acacttccaa tgcggcggaa gaacttaaac ctgatgacat cggcacatcg ctggtaatgg
    33661 ctaaatctgc tccttgtttg aattcggggt gtttggcttg aataaatttc accacgccac
    33721 gcacataatt cgcccatttt tgctcgcttg gctcaatcgc ctggcttaaa tcaaattcat
    33781 cttgttggtt gagatcaatg gcgaacaccc gccatttgtt gtcactacgt ttggcaaagc
    33841 taaccgctgt gccgtaattg atggcacaag gcattacaaa cccatcgttg taatcagtat
    33901 gttcgccgat aatattcact ctgcccgggg caaataccgt gcgttctgct ttataaccaa
    33961 aatgttgttc aaattgttgc aaagcgattt cttgtggttt catttcaatt ccttatttat
    34021 ctttatagtg tttggtatca cttactgcat ttaaacgttc ggcagcttgt tccggggtta
    34081 aatcacgttg gctttctgcc atcatttcat aaccgaccat aaatttacgc actgttgccg
    34141 aacgcaagag cggcggatag aagtgagcgt gtaattgcca gtgagcattt tccttgccgt
    34201 taaacggagc gaagtgaaag cccattgaat aaggaaagct gatgttaaac agattgtcgt
    34261 aacgggtggt gagctttttc aatgcaaggg ctaaatccgc tttttccgct tcgtttaaat
    34321 cggtaatgga tttgaactgc ttggctttcg gcaacagcaa ggtttcaaac ggccaagccg
    34381 cccagtaagg cacaaccgca atccaatctt cggtttctgc cacaattcgc tcttgtttgt
    34441 ctaattcacg ttgtgcgtag tcaagcaaca gtggcgagcc atattgagca aaatatttcg
    34501 cttggcattc gtcttcaatc gcaatttcat taggtagaaa attacttgcc caaatttgcc
    34561 cgtgtgggtg agggttagaa caccccatca ttgagccttt gttctcaaaa atttgcaccc
    34621 actgataatg ctctttcagc tcattcgctt gctcttgcca aactgttacc acatcagtaa
    34681 tttcctcgat ggaaagttgc ggtaaggttt tactatgatc aggcgaaaaa cagatcacac
    34741 ggctttcgcc ttgtgtatga gaaatctgaa acagcggatc atcattcggt attggtgccg
    34801 gtgtgtctgg tagcagggca gagaagtcgt ttttaaacac aaacggcttg gtgtaattcg
    34861 ggttttgttc gccggtaatc cgcttattgc cggggcagag gtaacaactt gggtcgtagc
    34921 tcggtttttc atcttgcacg atttcttctt gttgcccttg ccacgggcgt tttgcccgat
    34981 gcggagagac taaaatccat tgatttttta atggattaaa acgacgatgt gggtgatcgt
    35041 tgaggacaaa ttcgctcata taaaactcct cgaaattagt tatgtaaaga gtatagcgaa
    35101 agtcagacaa aatgactgtg acatagctca caaaataatg atgtcaagcg gtcaaatttc
    35161 agaaaaattt tgcaagtttt cctccatttt gcgatttaag gtagcttacg tggaattgaa
    35221 tgtggctttt ggtagaatag atgaatatgc aagatattaa gacaaaaata gatggaaaga
    35281 ctgaataaat ttgatgcttg gttaatgaat cataaaccta atgctaggtt aagcggagcg
    35341 agtcgtttgt tgacagagtt ttggttcttt gggttaaaag aagctagagc ctgtttattt
    35401 gcgggattat tttttattgc gatgttttcg gtaccaaaaa cgggattatg gggcttacct
    35461 cgttatgata ttttgctgat ttttgccata gtggtgcaaa ttgcaatggt gaaactgggg
    35521 ctggaaacgt gggacgagct aaaagcaata agcctatttc atttggttgg ttttgtgtta
    35581 gaagtcttta aaacatcgag taatattcaa tcttgggcat atccggattt tgcctatact
    35641 aaattgttca atgtgccatt atttactggt tttatgtatg cagcagttgg ttcttacatt
    35701 atccaagcgt ggcggttgtt tgatttgaaa attaaaagcc acctgcccta ttggttagcc
    35761 actttaacgg cactgatgat ttacctgaat ttctttacgc atcattacat tggggattac
    35821 cgttggtata ttgccgcttt tgcattaggg ctgtatgcac gtacttttgt atttttcacc
    35881 ccttatgaca ttacacgaaa aatgccactg ctgctttctt tcttcctgat aggttttttc
    35941 atttggttgg cggaaaatat cggtacgttt gccggaattt ggcgctaccc gaatcagttt
    36001 ggggcttggt ctatggtgca tatgagcaag tggagtgctt gggcgttatt ggtgatgatg
    36061 acttttacca tcattgtgca tttaaagcat atcaagcaaa cagtcagagt ctcgagcgat
    36121 taacaagctg tgggttttgg cgaaaatttt actcatcagt tattttcagt ggaattaggg
    36181 gcaaataata atttgcccct atatcaaatt atggattttc cccttccaca tcaataatca
    36241 tcacttccga ctgtgagcct aaccgcatcg gcacgcccca gaatccgtag ccggaggtga
    36301 caaaataatg cccgaagccg attttttcat agccgtaatg taatcgatag atcagtttgg
    36361 taataatact tgcagggaac acttgccctt tgtgagcgtg tcctgaaact tgaatatcaa
    36421 tcggcaattt agcatgttgc tcaatttcag tcgggcggtg gtcaagcaaa attaccggta
    36481 gtttagtgtt cacgttttgt aaaagctgtt cggcagaagg tcgatttttg actaaatcat
    36541 cattacgccc aataagggta aattttccgt taatttcggc cgtttgatcc cataaaacag
    36601 taataccggc gtcggtaatt tcttttgcaa tcgtttcttg agccccaaaa aagtcgtgat
    36661 tgccaagggt agcatacacg ccaagaggag cttttaattt ggctaaatga ggctgcattt
    36721 tatcggcaac ataaacttcg gtattgtcgt ccataatatc gcccggcaat aaaatcagat
    36781 cgactttttg ctggttgaaa atctctgcca gttggtcaag ttgcttggtt ccgaacagtt
    36841 ttcctaaatg cagatcgctt gccataccaa tgcgaagcgg tttctctaat ggtttatcca
    36901 atttcaccga ataatgtact actgttgggg tataagcgtt gtaatagcca agcccaagta
    36961 agccaaagaa tgcaaatggg taggcgattt tcagccagcg attgacggta gtattgccgt
    37021 tgaatatttt gtaaatacaa gcggtgaaaa aactgataaa aaatgcaaaa agtaacaata
    37081 ccaatatgaa tgccattatt ctaaatagcg caaaagtacg agtaagatgc aaaatcacta
    37141 atccgtttgc gactaagaaa gtgagaatac caactgtttt acggttttta cttgataaat
    37201 taaataacca gctgacagtc tttgcaaagt tgaataaaaa gagttgtaac accgcaatca
    37261 caagggcggt ggtaatgtag taacgatatt ccatattttt ccttcaataa attggctaaa
    37321 cccagccgat ttgtcttaat aaaaataagc cgatgctggc actaaacatt gagccgagag
    37381 tcgtaatgcc gatcagattt gcggctgcct cgccgtctcc gccatagttg cgtgtcattg
    37441 cgtaggtggc tgaagcaacg ggagcagaag cagccacaaa gacaatgcct agttccatcg
    37501 gcggaagttg aaacacaaat ttcccgagta gtagcaagaa aattggagca aaaatcagcc
    37561 gaatcgaggc agaaaacagc acaattttat tgatggtgct ttccactcct tgattaaatt
    37621 gtttcaactg tttgaaatta atgcttgctc cggtgcagat aagtgctaat ggtagtgtca
    37681 ggctcgccat taatttacca gcagactcaa gcggggttgg aatttggtgg cttaattcaa
    37741 aatagttaaa agttaagcct gcaatgacgg ctagaatcag cggatttctc aatatcgaca
    37801 atattaatct gccaaaattc acttttttct cgcttaacga tgaagtgagg gtaatcactg
    37861 caagcacatt aaaggtaatc accaagcacg aaatataaac tgaagcggca gcgagagctt
    37921 tatcgccata agcattgaga attaaggcaa tgcctaaaat cgccgcattg gtacggaaag
    37981 tgccttgagt gaaaatacaa cgataacggc ggttttcaat atgtcgagcc gcccaccatt
    38041 ccgcccataa atagatcaaa atcgaaccgc cgactccggt cgagatcagg ttgatttggc
    38101 tggagaaatc taacgggctt tttagcatat tcaagaaaat catcggtggc agggtaaaat
    38161 taaataccac ctttgatgcc atattgcaaa aggtatcgtc aataaatttt cgtctgcgtg
    38221 gaaaaatacc gaacagcatt aacaaaatgg ttggtaaggt aacgccaatg ctaaattgga
    38281 tagagtcgaa aagcataatg tttgccttaa atgatggcac gagtgtcttt tgcttgtacc
    38341 gtagattatc aataaagtac cagcgtggac gctggtacta tcatcaatgg aaatgaaatt
    38401 acaatcctgc ctttaagctt gcttcgacaa atttatccaa atcaccgtcc agcacttgtt
    38461 gggtggtgcg atgttccaca ccggtacggt ggtctttaat ccgtgaatcg tctagcacat
    38521 aagagcggat ctggctgccc cagccgatgt cggatttgtt gtcttccatc gcctgttttt
    38581 ccgcattttt cttgctcatt tccagctcat atagcttcgc tttaagctgt ttcatcgcct
    38641 gatctttatt ttgatgttgt gaacgaccgt tctggcactg caccacaatg ccgctcggta
    38701 tatgggtaat tcgcaccgcc gattcggttt tattgacgtg ctgaccaccc gcccctgagg
    38761 cacgatacac atcaatccgc aaatccgcag ggttgatttc aatatcaata tcgtcatcga
    38821 cttccggata aacgaaagcc gccgagaacg aggtatgacg accgttattg gcatcaaacg
    38881 ggcttttacg caccaagcgg tgaatacccg tttcggttct gaaccagccg taagcatagt
    38941 cgccggaaat tttcacggtg gcggatttaa tccctgccac atcaccgtct gaggcttcca
    39001 ttagttcggc tttaaaccct ttgctctctg cccaacgcaa atacatacgc agtaacattt
    39061 ctgtccagtc ctgagcttcc gttccacccg aaccggcttg caaatccaaa tagcaatcgg
    39121 cactgtcgtg tgggccgctg aacatccgct taaattctaa atcggcaagt tttttctcta
    39181 attcgtctgc ttccacttgg gcttcattga aggtatcctg atcttctgct tccaccgcca
    39241 gctcaatcaa cccttcaaca tcttcaatcc cttgctctaa ggctttaatc gtattgacga
    39301 tctgctccaa cattgcccgc tctttaccaa gcgcttgtgc tttttccggc ttgttccata
    39361 catcgggttg ttccagctcg gcattgacct cttctaagcg ttcgacttta tgctcaaagt
    39421 caaagatacc ccctgatcac cgcggtgcgc tcgcttaaat cagttagttg gtttttaatt
    39481 gggtttaatt caaacattgt cgttcctgtt tttgcttatt ttaaaaagat gaattatagc
    39541 tgaagttaag caagcggtcg aattttgcaa gaaatggagc aatgatcgtt tctagtaatg
    39601 agatttggtt cataaaagtt gcaactgttt ctgctatttg gctttgatag ctaaaaatct
    39661 ttaaaaaaac aagcggtcgt tttaccccat tttttgcaaa acgaccgtag cttcttgcta
    39721 taatcgctga aaaaatgctg caaggagatt ttatggcaaa gcgcccaagt gatattgatg
    39781 caaccctatt gcaactggaa atcctacgcc gtatccccaa attacccgct aaaattgatg
    39841 ctaaaagttt gcaccaacag ctattagatg ccggctttga acgggatatt cgcactgttc
    39901 agcgttcgct caaaatgtta tgcgagcatt ttgatattga atgcgatgag cggagcaaac
    39961 cctttggtta tagctggaaa atccgctcag aggggctggc attgccgatt ctcaacgaac
    40021 aacaatcatt actgctaaaa cttgccgagc aacagctgaa atatctgtta cccgcaaatt
    40081 taatggcatc aatgaaaccc ttttttgagc aggcagacag aatggtttcc ggggcgaaag
    40141 acaagcccga ataccaatgg ctaaataaag tttgctccac gccaaccagc ctaccgctga
    40201 tccctgcaaa aattaaagaa gagattttta attccgtaag ccaagcctta tttcaaaata
    40261 agttgttaaa gatcgaatat cagaaccagc acggcaaaaa acaccaagcg acaattatgc
    40321 cgttggcgat ttcacaacaa ggtgcgagca tttatttggt ctgccgttac gaagggtttg
    40381 acgacaaccg cctgttggct cttcaccgca ttcgtaaagc ggagatttct acttttagtt
    40441 ttgaacgccc gaaagatttt aatttgcaag cctaccaaga tgccggacac ctcggttttg
    40501 gcaatggcga aaaagctagc cttacctttt cgattagcag agacgccggt ttccatttaa
    40561 ccgaaacacc gcttgccaaa gatcaaaaaa ttctcgagga gagtgaaact cactaccgta
    40621 ttcaagccat agtcgctgat aatgatatgc taacctggta gctacgcaaa tttggcgaag
    40681 atatttggga tatcgagaaa gaagcgctaa aataagctaa acaagcggtc ggttttgtgg
    40741 gaatttttgc taaatagtat cgaccgctcc tccctaaaca gacctttatc ataaatacaa
    40801 ctctttcaat cagatattct tactgtctat tgatgaacga ttcgaatttt tctagtggtt
    40861 gaaatttaaa ctaattttct tgctttttgg catttttcag acttttaatt ctctccatta
    40921 gcctgttttt ttacgctctt tttcataaac aggaataacc aatgcctttt caacttactt
    40981 tctgccaaca agccggcaat cagcaccaga aaaatcaaga tgcactgttt aacggcaaag
    41041 cggtgtacca atggcaactt aaaaatgccg agagttatct gctggataac gacaaagtaa
    41101 tttttggggt tgcggacggc gtttccagcc accctcgaaa tcatcttgcc tcacattatt
    41161 taatggcgtt actcagccaa tgccaatccc ttgatagcca atggttgcga caggctcacg
    41221 gcgatttttg cgaaaaatta gcgaaagatt attttggtgc ttcttgcact tttgttgctt
    41281 gtgaattaca ccataacggc agagggcgta tggttaatgt gggcgacagt cgagcctata
    41341 aaattacggc aaacggggag tggcaacaac tgagttttga tcacaccata ctggctgaaa
    41401 tgaaacagaa aggcttggtt aaggaaggga gcaactatgc ctcaatgtat aacggcttaa
    41461 ccgattgtat tgttgccgat tttgacgccg atgatttccg cattcatata gccgaatttc
    41521 aactggaaaa aggcgagagc attttgctct gttctgacgg tttgcacgaa caactcgccc
    41581 cagaagctat tcaggcgatt taggacggat acgaaagccc gtctcatcgt ctgcaaattt
    41641 gcagaaaaat ggtaaaaatg caccgcttgt atgacgattt ttctgcggtg atttgtgaaa
    41701 ttgtgtaatt aaaacgacat tatttgtcgc attggtgctt aaaatccgca taaaacagag
    41761 gagaaaaaaa tgaaaaacga tttccattat gccgctgaat taatccgcaa tgcagacggc
    41821 attttaatta ccgcaggggc tggaatgtcg gtggacagtg gcttgcccga tttccgtagc
    41881 gtgggagggt tatggaattc ctacccaccg ctcaaaaaat ttaatttgca atttatgcag
    41941 attgccacac cgcttgccta tcaggaacgc cctga
//
LOCUS       DANLXQ010000004        37873 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.004, whole genome shotgun sequence.
ACCESSION   DANLXQ010000004 DANLXQ010000000
VERSION     DANLXQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 37873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37873
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.004"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            46..264
                     /locus_tag="PXD94_000151"
     CDS             46..264
                     /locus_tag="PXD94_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6285060.1"
                     /translation="MARNKRVRDMSNFEIRGELMKIGKSLSQLGIENGLAKTTVRNAL
                     DKPYPKGEQIIAKAIGKTPQEIWPSRYE"
     gene            274..2244
                     /locus_tag="PXD94_000152"
     CDS             274..2244
                     /locus_tag="PXD94_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HDL6285061.1"
                     /translation="MKEWFLAKEIAGLSGVPELPNSVSRLATKEGWQKRQIQGVRGVT
                     YEYHLTSLPIETQQQLRLNAALAVIPQAAELQPKRDDPALIARLNNATDKGRDKAKGK
                     AEACMQLQAFLDQGFSYTQAEAGAATAKNVSQGSLKNWYYKVKGHPVHLWQAILISES
                     GRSKKPQLKAKITEEAWDCFLADYLRPEKPDLRASYRRTQAIAKQYGWQIASLQTFQR
                     RVLAEVPYEVILLKREGANAVAKLVPALQRTVKDILAGEWINGDGYQHNVFVKWHTGE
                     IVRPKTWFWQDVRTRKILGYRTSISENTDSIRHALMDVIFNVGIPKTLTLDNTRAAAN
                     KAMTGGIENRYRFKHTELDPKGIMPILGIDVHFTSILYGEGHGQAKPIERAFGRGGIG
                     EKIDKRPELSGFYTGRNAQETPDNYNGGKDGVDYNTFLKAIAAGIEEYNSQTERHTEM
                     CRGELSFNQVWERDYHPSNVRQASPEQLRLLFLQAETVSIKRNGSFTLKAAGKLYGLT
                     NVYWAESLIGITDKKVVARFDPDDLHGNVYVYNLEGQFLAEAVCREAKGFGDTSASRE
                     QGRLYQKVVKSAKAQAEALELLEAHELASLAPQVDVPEPIEKKVKEVLVKEDFIVDFN
                     TVRKATVVEETEEISIFDEVLLKQEKQLRIVE"
     gene            complement(2424..2912)
                     /locus_tag="PXD94_000153"
     CDS             complement(2424..2912)
                     /locus_tag="PXD94_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285062.1"
                     /translation="MLFRSLTDDEIHQIALDSARATFSGFKLKRANAIVMKNCITLFK
                     LSTINFCLAEYHSSFHHLTSIDDLDGIMPTVKKVAQMLSLQESKALIWLLYLKWLFVM
                     RQRSLQVLLENSADLIQGEVIAQPLPTFVKAVLAFQLETYHGLPEWIPEFDSAEFWQS
                     LS"
     gene            2944..3885
                     /locus_tag="PXD94_000154"
     CDS             2944..3885
                     /locus_tag="PXD94_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6285063.1"
                     /translation="MTLIDQIKQHIAESGSSQGKVAKEAGINAGALSAYLNENYKGNN
                     EELEAKLIAYLERIEAKKREFVEAPSFIETKTANQIFGSLRFAQNTGVLAIIHGASGV
                     GKTQAAREYRKRYANVWLVTASPSRSSLSEVLYEIALELGMNDAPRRKGTLARLIVRK
                     MKGTAGLLIIDEADHLPYEALEELRLLQEEVVNESDIGAGVGLVLIGNDKVYTRMKGG
                     INPAHEYGRLWSRSAKRTSIQKTKKEDTQAVAKAWGLSENQEALKVMQAITETGGGLR
                     ILTHTLRLAAMVAKDRMIPLTADLIQLARKDLLSTGD"
     gene            3888..4202
                     /locus_tag="PXD94_000155"
     CDS             3888..4202
                     /locus_tag="PXD94_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285064.1"
                     /translation="MRTPKPKHSFNRTNQAACRFLKQTQKAIIRLNSLGFTVLNIDFT
                     RIKPRIEVEIGNNKHIAQGLIYEGKAYRYSFGKSEDLGRWEGYYTMLEGIRVWWRQAA
                     IH"
     gene            4214..4450
                     /locus_tag="PXD94_000156"
     CDS             4214..4450
                     /locus_tag="PXD94_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285065.1"
                     /translation="MKTLALLTALLGLVGCDLVQSKQAKNYIGNNLTEICIDNVVYLI
                     YSGDKKGGITPKVNKDFYPYTCTNKEERQNGKTR"
     gene            4434..4727
                     /locus_tag="PXD94_000157"
     CDS             4434..4727
                     /locus_tag="PXD94_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285066.1"
                     /translation="MAKPDETPITKAECQSQLAELGVQYKKLPMAITRHICNATTNIH
                     GKVIKVSVVERVGYGVQITAQGNEKSCLVTYEAMLGMAEAMGLFDEVKEQNND"
     gene            4849..5067
                     /locus_tag="PXD94_000158"
     CDS             4849..5067
                     /locus_tag="PXD94_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL6285067.1"
                     /translation="MNDELAAISASYAQHNRYQHYAEKAAEAERKGNYKEAQDHWEVA
                     KLSAKKTANRDWAEQRAEFCKRMHNKPF"
     gene            5077..5259
                     /locus_tag="PXD94_000159"
     CDS             5077..5259
                     /locus_tag="PXD94_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285068.1"
                     /translation="MTETVKAQLNSQLNEAIIQLIQAQKYLNQDDAIRSGVYIGTVQD
                     LLPKVHLKLLTANRKH"
     gene            5276..5665
                     /locus_tag="PXD94_000160"
     CDS             5276..5665
                     /locus_tag="PXD94_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285069.1"
                     /translation="MAITEEQWKEIKQKLDSIIGRVKFRYKEHLLTVDVVQIKRSLKL
                     AVYVDGEIDGAWTKEGHEIRPYLEEVWYRKERPFFNAKEKKEYRGLMSKKKLNEKIVT
                     YSPMFPSPTALTRQYKKLDGLELIEVI"
     gene            5714..6001
                     /locus_tag="PXD94_000161"
     CDS             5714..6001
                     /locus_tag="PXD94_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285070.1"
                     /translation="MNKNEFFKKYHLEGSLAILDLDANDDQSQFCYQALLEYRELPLD
                     TFLAEYSDDEDMTESEFIELMLDELEWIIRRAKNAKRFVKKRQKTMREEIM"
     gene            6120..6665
                     /locus_tag="PXD94_000162"
     CDS             6120..6665
                     /locus_tag="PXD94_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HDL6285071.1"
                     /translation="MTDKAKLIQLIHIAKSQLAMDDLSYREMLKRLTNKTSSTKCTVV
                     ELHKVLHELQTKGAKVKYFAKRSKKPTAYSPATGETAVKSQIAHKIRAVWITMGKHGF
                     LNDPSEKALNAYMRKVINNKTRPTILLNVSALEAGDASYMLEILKKWHKRVMIKALSK
                     SCTVDPKISYDELVEWYNEVM"
     gene            6652..7185
                     /locus_tag="PXD94_000163"
     CDS             6652..7185
                     /locus_tag="PXD94_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285072.1"
                     /translation="MKLCRCPICHSDIHLEALVEDEAGRELLGKISQLTHGVAQPMVA
                     YLGLFKPQKSNLNNSRALKILNDVLALYPCSLLLAQALSETVVSIRKKRQQAMENGQK
                     IEPLPNHNYLKSVYETQKVHFAVVRTGKTERETMKAELEKTRNAIAYVQRFVDLGREE
                     VVKNSPEYQIWLAHKPK"
     gene            7350..7709
                     /locus_tag="PXD94_000164"
     CDS             7350..7709
                     /locus_tag="PXD94_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6285073.1"
                     /translation="MELVDIFEEKAPEVLTDLAKHIEVALEEKCQFSPEKAKQIGIDI
                     AQQMAQNWGGEVIYIPRNLLMALNERDMKIYEEFNGNNHRELARKYNVSMQWVYKIVK
                     KVHKDEVARRQMSMFEM"
     gene            7845..8354
                     /locus_tag="PXD94_000165"
     CDS             7845..8354
                     /locus_tag="PXD94_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL6285074.1"
                     /translation="MSQNLTFRQIFDRLIGHEGGYVNHPQDPGGETHWGITKRTAVAN
                     GYMYAMKNMTREQAYQIYEKAFWQRYRCAELKPAVAYQFFDAVVNHGFGNASRMLQRA
                     VGVADDGIIGNITLQAINAKSESDLLMLFNAERLKFYTKLRNFPTFGKGWVNRVAGNL
                     EYAAQDNEV"
     gene            8357..8626
                     /locus_tag="PXD94_000166"
     CDS             8357..8626
                     /locus_tag="PXD94_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL6285075.1"
                     /translation="MAKLFKELLSTNGTLSTTSTVQFFGTLCVFGLMVYAVITQQPYA
                     ESLLNNVLIYLFGATTAKGVVTSYQAKIKGGIHKQQSTFKEEEGC"
     gene            8620..8967
                     /locus_tag="PXD94_000167"
     CDS             8620..8967
                     /locus_tag="PXD94_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL6285076.1"
                     /translation="MLMQQILGVIGVIAAILSYALFKSWQLKRERKKNAILQAEKQQQ
                     AVEIEQKKAEVKYVYISKKNNEKVTRSSASDIDDKLQQHGYFRDNNRLHGVQSDLSEP
                     CGYAGNETSDSCS"
     gene            8994..9146
                     /locus_tag="PXD94_000168"
     CDS             8994..9146
                     /locus_tag="PXD94_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285077.1"
                     /translation="MKYIKKLRKRWQIWRFLKNHPEVAQRRDLLAIAIKQGVENPVGR
                     ARRREP"
     gene            9146..9481
                     /locus_tag="PXD94_000169"
     CDS             9146..9481
                     /locus_tag="PXD94_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 domain-containing protein"
                     /protein_id="HDL6285078.1"
                     /translation="MDLFTVFWESIRANFGFISALLTIVVTAFWLKLDSKYAKKNDLG
                     ELLEIAKSHDNRLTTLETKVENLPSAVDVEKLKTLVTDVKGDTKATSKQVDSISHQLG
                     LLLEAKLKE"
     gene            9483..9779
                     /locus_tag="PXD94_000170"
     CDS             9483..9779
                     /locus_tag="PXD94_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285079.1"
                     /translation="MSLRDLLTQDQRLVILRSLAEAGYDANESIISDCLDLYGHDISR
                     DLVRTHLSWLEEQGLLTLERLRDGYMVASITQRGLDVAQGRVVVSGVKRPRPKI"
     gene            9802..10374
                     /locus_tag="PXD94_000171"
     CDS             9802..10374
                     /locus_tag="PXD94_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDL6285080.1"
                     /translation="MSEKNTRGRASKVDLLPPNIKTQLAMMLRDKQYSQSQILEEIND
                     LIRDCGLPDEMLLSKTGLNRYASRMERLGAKIRNAREVAEIWTKQFGEMPQTDIGKTL
                     MEMVKQIAFETSLKLGEQEGGIEPKQLALLSSAIQRLEQAESLSFKREQAIRKEVAQQ
                     AAETAEKVVVQAGLSAETVRTIKEQILGIA"
     gene            10374..11930
                     /locus_tag="PXD94_000172"
     CDS             10374..11930
                     /locus_tag="PXD94_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDL6285081.1"
                     /translation="MALLNNRPLNELAPECRSFLESIHAFNPNELLLGYQKRWIADDS
                     QLKIAEKTRRCGLTWAEAADNALIASTRKSDGGSDVFYIGSNKEMAREYIDAVAMWAK
                     AFNRVAGEIQEEIFEDEDKDILTYVIYFSSGFKVKALSSNPKNLRGMQGVVVIDEAAF
                     HEYLAEVLKAALALTMWGAKVRIISTHNGSENLFNELITDSRAGRKRYSVHTITIEDA
                     CRDGLYQRICQVVTKKTWSPEAEKEWIENLLNDTASEEDALEEYFCVPKFGSGLWLSR
                     ALIERQMSEATPVIRFTAKDGFSLVAEPTRYAEIAEWCELELEPILATLNPDLLHFMG
                     EDFARSGDMTSLVVLAQQQNLVRKVAFIVELGNMPYKQQEQIVMFMLKRLPHFAGGAF
                     DARGNGGYLAEAARDEFSTLIDCVQLSEKWYREHTAPFKAALEDGELEAIPKDADILA
                     DLRSFQVVKGVPRIPDKRTKSADGKSKRHGDTAISLLLAHYASRQLVQMPVEPVSRRP
                     RHSQQLTEGY"
     gene            12049..13503
                     /locus_tag="PXD94_000173"
     CDS             12049..13503
                     /locus_tag="PXD94_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HDL6285082.1"
                     /translation="MAKKNNLISEIATRARSIDYWAFGYYLPNPDPILKRMGKDISVY
                     RELLSDGQVRSGVRRRKAAIKGLEWRITTTNNEKVDEQIYQIFNRLPMNNLITEMLNA
                     ALYGYHISEVIWAKEGELFVPTEIIGKKPEWFMFDDENLLRFRTKENWADGELLPDDK
                     FLLTTQEATQDNPYGLGDLSLCFWAATFKKGGFKYWLEFTEKYGSPWLIGKHPRQAHN
                     TDKERLADALEGMISSAIAVIPEDSSVEIKDAAGKSASTDAFEKFLNFCKAEINIALL
                     GQNQTTEQESNRASAQVGLEVMEDIRSDDQAMIEATFNTLLQWIVKYNFNVEQLPKFE
                     FYEQEAINTDQVERDSKLHAMGVRFSKAYFEREYGFEAGDIEVQAVEKAEKSANTTEF
                     SEGQRRTQVPMPVPILEQLEHEGEAMVDDWLQGVRDKLAQADSLEDFQSQLDSLIPEL
                     SFAEYGELLALGSTVAELAGRQSVNDEIKAKRNA"
     gene            13496..14773
                     /locus_tag="PXD94_000174"
     CDS             13496..14773
                     /locus_tag="PXD94_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL6285083.1"
                     /translation="MRKFTFKQQAQYFEKKLNLRTNSYLDILGEEHDYFFVVAGANRN
                     EVLSEFRKAVDEAIHNGETLENFRKRFDEVVAKTGWEYNGGRNWRTRIIYDTNVYGAY
                     NRGRLQQHLELAEDMPYWEYQHNDNAHPRPQHVAWDGLVLRYDDPFWKYHYPIKAYGC
                     HCTVQALDDFDLKHDGKTVSPPPEIEFEQKTVGVRTGNSRVVTVPKGVDVGFTPHNFD
                     NLKAGRAANVDKLLMQKMVTADPIFASKAVSDLLAKEPVAKLHNQAFKNWVNDVANAK
                     GSALSQVENMQLLGVLPVAVIERLQQINLAPQSAIIAITKGDMVHALRELKQNTGIAV
                     PLEFWEKLPAKLQYPNAILLEKQQAETTLLFIYDVAQTKIAVKVNYDATLQDRHGAKG
                     RQKVLLNRAVTGSLFQDTTGLKSSKYEVLLGEM"
     gene            complement(14975..15319)
                     /locus_tag="PXD94_000175"
     CDS             complement(14975..15319)
                     /locus_tag="PXD94_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285084.1"
                     /translation="MMVNAEQAIELVYDLLLSRQWLVTKAEKLPLDPLSEKEAVMFLY
                     TLDQQTEASWLQLTPEQRATANGLIMDFIAKCLTSTKQWLVSDNIVPELQAIEIIKHE
                     IFLSHNSLVMPN"
     gene            15383..15847
                     /locus_tag="PXD94_000176"
     CDS             15383..15847
                     /locus_tag="PXD94_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL6285085.1"
                     /translation="MIKITLNNTQAVKSLQQIANQLEQPRRLYGILGETLKKIHTARF
                     KAEQDPDGNNWKPLASSTLALKRKRGKSTKILRQDGYLADKTAYNVTSDNVEFGSQEV
                     YARLHQFGGKAGRGKKVTVPARPWLGVGDKDEQLLLRKAEWHLGQIIGRMGK"
     gene            16082..17197
                     /locus_tag="PXD94_000177"
     CDS             16082..17197
                     /locus_tag="PXD94_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL6285086.1"
                     /translation="MTQIEIFKAGKRYDANGTLIDITPEMLQQTVAAYNPEFHEAPLV
                     IGHPKSNNPAWGWVKALSLEGDVLKAEVDQLDAEFSEMVASGKFKKVSAAFYLPDSPN
                     NPHKGVLSLRHVGFLGAMPPAVKGLKQVEFAEDDDFVVFSEWGVASLFSRFRDWLVSE
                     FGLEKADKALPPHEIDWLKEEAMRSQISKQVHAEQATPEPIFNEPAQPEGEPEMSAEE
                     KEELERLKAENEQLKAEKAQAEAEKMEAELEAEKAGNAEFCEGLVKQGKLAPVAKAAF
                     VQALNNLSVLKAGREPEFNEGEDVVSQFKSAMSQSPQIIQFGEYATADKANPQEAEAV
                     EYAEGTDPVSIEADQKVRAYMKAHNVGYAAAFNAIYR"
     gene            17228..18154
                     /locus_tag="PXD94_000178"
     CDS             17228..18154
                     /locus_tag="PXD94_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDL6285087.1"
                     /translation="MTTHNLQKLRVQDPVLTELAQGYHNNELVAETLMPTVEIEKEAG
                     KIPTFGRLAFRLPSTVRNLRGSSNRLDPEDIGAIDVALEEHDVEYAIDYREENEAIFS
                     LRQFALNTTQDVIALGREKAVATLALDESRYETDNKVTLSGTSQFSHKDADVFKVFDT
                     AIRAVKRAIGRKPNVCVISGDVWAALKEHPAVVEKLKYSQVAIVTPEVFAKLIGIETV
                     KIGEAVYEESGQLKDIWSKDIVVAYVAPRSTGGKGTVYEPSYGYTVRRQKGLFVDTYK
                     ESGGKIEVVRTTDIHKPHLVGAAAGYLIKAAV"
     gene            18223..18504
                     /locus_tag="PXD94_000179"
     CDS             18223..18504
                     /locus_tag="PXD94_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285088.1"
                     /translation="MAKFIVLHTPILHDGKRYEVGAEIELTAEQAENNAINLKAVEAV
                     PKPPKNASKPEDEPKPEGEPKPEDAVTEDDVKLAEAKIAEAEQPKGNKK"
     gene            18504..18938
                     /locus_tag="PXD94_000180"
     CDS             18504..18938
                     /locus_tag="PXD94_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL6285089.1"
                     /translation="MYVQIDELLTAFSRKIVVQLSNDEPTATEPDLKMLETAVKVANE
                     RIDAALRSRYTLPLADVPTLINQHALTLARYWLYARRPETKMPDTVKETYQQAVKELE
                     QIATGKLHLGIAESSQILEDDVLADNSEYEVRANARIDTSRY"
     gene            18944..19441
                     /locus_tag="PXD94_000181"
     CDS             18944..19441
                     /locus_tag="PXD94_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDL6285090.1"
                     /translation="MSATQPILEDICQHLRQALPEWEVELFPNDPSTYHLSHINGAVL
                     ISYLASKFEKPRTTQAVLQVRHVQIALTVLTRDLHNDVGALDLLDKLRLLVVGFKPQN
                     CGECWLIDEFFNGSSEETGLWQYQLILQTETQQVQHQAVENLPKFVTAHLRRQDQPGS
                     PGLKT"
     gene            19526..20911
                     /locus_tag="PXD94_000182"
     CDS             19526..20911
                     /locus_tag="PXD94_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL6285091.1"
                     /translation="MTFHHGSETERVNGGSVPVSVVDSAIIGIVGTAPIGAVNELTVC
                     LTKKDFAKFGTILNQGFTLPDAFDILARYAAGKVYVVNVLDPSRHRTTVTDEVLTLDR
                     NTLTAQTAKTGIIAVTRVKDGANVLNADRYSVDLQTGVVTFVSAPSAPKISYIYADPS
                     KVTEEDIKGGVDSLTGKRKGFELLRDGFNLYGADAKILICPEFDKTASCAAALATLAD
                     QLHAKAYIQLPKGTSLSKAIQGRGPAGTINASASNENVRHFFPYALGSSNTLESLATH
                     AAGLRMLIDVEKGYWFSTSNKELKGVIGLEIPLTARVDDKQSETNLLNAVGITTIFNS
                     FGTGFRLWGNRSSNFPTVTHISNFEVASRTGDIIDESIRQAELQFIDLPIDDALLDSF
                     LETIDTFLRTQKSLVGYSVSLDYEYDLVDAFSRGQVPIVYEYTPKIPGERISNKSVMT
                     RKYLANLVSQR"
     gene            20922..21437
                     /locus_tag="PXD94_000183"
     CDS             20922..21437
                     /locus_tag="PXD94_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL6285092.1"
                     /translation="MSIAINQIVNGNVYINGNSQMGRVNEVKIPDIEFEFIEHKGLGM
                     HGVIKLPAGTNAIEAEITWDSFYPEVRAVMLNPYKYTQLQIRSNLQVFNSQGLAAEEP
                     MVTIMNVSAGKIGGTGHKNKENAEFADTLNVHSIKQTVAGRELLFIDVLANIYRVNGV
                     DVLQKFRTNIG"
     gene            21533..21847
                     /locus_tag="PXD94_000184"
     CDS             21533..21847
                     /locus_tag="PXD94_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL6285093.1"
                     /translation="MANAAQQVLTNLRVQTTYKLQYPIALPDGRVIETLDVRRPKGKD
                     FRLLDEKGFDPEKDGVKILRFYIQQLTTLVPEDIDELDAADIKGLNNLLEDMLIEGKS
                     DK"
     gene            21844..21996
                     /locus_tag="PXD94_000185"
     CDS             21844..21996
                     /locus_tag="PXD94_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL6285094.1"
                     /translation="MSELDTVYADLAWWFKFSPSELLELDLVDIPKWVEQMNRQIKAG
                     YGQILR"
     gene            complement(21975..22277)
                     /locus_tag="PXD94_000186"
     CDS             complement(21975..22277)
                     /locus_tag="PXD94_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285095.1"
                     /translation="MLTKLNNRITEWAGFEACVTRTECTAKWLLAVALVGWSIFYWLD
                     AIQSIQSLSEGVSFVLIYGLILTILAWVGVYLAWGVAILLTLATEPFIWLYRKICP"
     gene            22325..24982
                     /locus_tag="PXD94_000187"
     CDS             22325..24982
                     /locus_tag="PXD94_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6285096.1"
                     /translation="MANNLAIGMVIGASLSSGFHGAFSGAKKKLEQLEQAVAKNQQSH
                     SKLGSELTALRSKQATLYAEMSRASSKGGAGLALMQQEYKKIGKEIANISREQRKWST
                     ELEKSIAKQTKLRNAMERHTQAKENRDELKGKIIGTVASAAMGVGVMKTYMEQEEAAN
                     NLKISMLKADGSFGKFEEIGKIADTLGTDLPGTKKDFYKLAMALKKQGVSDDVLTGGA
                     LKTSAELNVLLDMDQESGGEFLAKFMESHRLNENELPQAADYLQRAMFAGGLSKEQMY
                     ESMKYYAPKLNSMKLTGAENTEKVLAIEAMAGQQGLEGSTFGTGLNMMLSRMNKGPEM
                     LKAAAKGMKAEAREMMEAVGVEFNFWDKNGSFKGIDGMLAEMTKFEKIRKKFGDKGVG
                     LVAEELFGIEGGRLADILAMKGQKGLDEMLSKMREQASLQDRINLKTNTLGAALESLG
                     GAWETFVGDIGSVFAEDIKGFSKGLQSAVEWLVPFVSKHKEAIKFVGGLVAGLVILRG
                     VLQAGSFVIQTVTMAMNALKIAAMTNPIVLVIGAIAAGAYLIYSHWDSIAPWFANLWQ
                     NVTAYFNQFWTWVKGVWSGVTDWVSSVWNGVATFFGQLWTTITSLFSVAWTNIQTIWG
                     GATAWFSGLWDGIKSAAFGMFDSISQKVSSVVASIKSFLGFGDDVDKMGAKIGNVATQ
                     VISKEHSGQLSKTADMAANTGFSSGGFTGQGGKYTPAGIVHKGEYVMTKEATSRLGVG
                     MLDSLNYGGAMGFTQPVLQPILGKQKGFFGSLWDDVKFGANFVGNLLGLNQGSLKTPD
                     FNPNATNASGQNSSIFSNYEPLNRNAVTQQESSQAPQIVVNFNPTINVNGNATQEAQS
                     QIMQQLQMSAYEFEKLLNRVLDQRSRRAY"
     gene            24992..25915
                     /locus_tag="PXD94_000188"
     CDS             24992..25915
                     /locus_tag="PXD94_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285097.1"
                     /translation="MYFMLGNIAFEPVNLTEFSEQHTAEFAEHAVLKGKPRLQAMGEK
                     LNELNFSLRLHHTIGGVESRYQALLEAKAKQAALALIWGSRYKGDYVIVDISSTTLFT
                     DGRGNALAREMQISLKEFVGDNDGGVLGEALNFGGGSLLGSILPSGAVTTLSQIKTAV
                     SRGVELYNSGKRLVDEVQNTVAIIRQLKNDPATALAYLPAALANLDGALGHFGNLTGS
                     SATLGGLQEKLPAAVVFSEEIGEIYQSLQTMRSSLGNASANSWDNWFTPAESALTEVT
                     DSFDHLAKPVAKMTAWIVLRTDEEAENDTVA"
     gene            25899..26126
                     /locus_tag="PXD94_000189"
     CDS             25899..26126
                     /locus_tag="PXD94_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL6285098.1"
                     /translation="MTQLLKHIVKQGERWDNLSYQYYGNAFEYGRIIDANPHIGFCEV
                     LPIGVTIYIPVLNVKPTNNENMPPWLRGRDA"
     gene            26119..27183
                     /locus_tag="PXD94_000190"
     CDS             26119..27183
                     /locus_tag="PXD94_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285099.1"
                     /translation="MRNVPKPDFSLFYEKTNITAEIEPHLVQLTYIDHLEGQSDELTV
                     EFEDIQGKWIRQWFPTQGDKLKAAIGYQGEFLTDIGEFEIDEVEYSYKPSTINLKALS
                     TGISKANRTLKPKAYENTTLAQVVAKVADNLKLKVVGKIKAIPIKRITQYQERDVEFL
                     ARLAREYHHSFKIVGNQLVFTDKSELGQSEPVLMLEERDTISLRLRDRIKDTAKAVEI
                     SGYDTSGKKVVKKRKKAKALRPNIKQAKSASEDTLKIITRGESQEQIDARGEAALATQ
                     NEDQAAGDIELIGNPKLVAGATILLRNFGVFSGKYLIKSSRHTISRSGGYTTHIEVRM
                     LEFIADDLITLGMEAGNANA"
     gene            27170..27706
                     /locus_tag="PXD94_000191"
     CDS             27170..27706
                     /locus_tag="PXD94_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL6285100.1"
                     /translation="MQTHNFGATYQEGIVSAVDPKTHKVRCKIPALEDLETAWLSFLT
                     PNAGGNQFYCLPDVGELVAMLLDAQGEGGCVFGAIYNEQDPTPVADSDMWVKKFSNGT
                     EISHNRKTGDVVVKTNGHLTATAQGGATINANTVVNGTLHATGKITSDEEVSAPVVKQ
                     GTVELGTHKHGGSPEPNK"
     gene            27761..28123
                     /locus_tag="PXD94_000192"
     CDS             27761..28123
                     /locus_tag="PXD94_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL6285101.1"
                     /translation="MNRQTSIQLTHWQLAPSTDTVEVVQGIDDIHLCIVNILSTQKGT
                     DILRPEFGSDHFRYIDYPEDVAIPNFVREITTALQKWEKRIEVEEVLVDGSAPHFIFT
                     VMWHLTDDVYREIYRTTV"
     gene            28133..29236
                     /locus_tag="PXD94_000193"
     CDS             28133..29236
                     /locus_tag="PXD94_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL6285102.1"
                     /translation="MTEAIKIIDDDVKTVLAEAIADYEKRTGKILQPAHIERSIIQTY
                     AYREQLLRKGINHAFLQTFPQFATGIALDLCGETFGCYRLRDKPARTLLRFSVSGEHT
                     AIVIPKGTQVAINDELYFSTLNDDVITPLITYVEIDAECNQVGVIGNGWESGRVSKLK
                     TALATNYKVTVENIDITSGGLMQEEDEPYRKRILAAPEAFSNCGSIAAYDYHVRSVSQ
                     DIADVNISTPRGGLVRIAVLTQKGLPDTRLLDDIKKHVSAERLRPLCDMVEVIKPTER
                     RYQITARLILLDGYREDLVKAKAREALQLYLSDKTKKLGLDIVPSAIISALRVEGVYD
                     VNLIQPVKTIINETEWANCTAISVEVEEERRDG"
     gene            29229..29795
                     /locus_tag="PXD94_000194"
     CDS             29229..29795
                     /locus_tag="PXD94_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285103.1"
                     /translation="MASLHYADIIVRDPKYKALADLSKRLNLLDTSQIMTTLVDLLGD
                     ECIPLLAEKWSVTGYDGLLVADSENSKRALIKRAIELHQLKGTPWSIREVLRRLGFGE
                     VEIDEGLKNRLYESTYVSGIPENERWAHYAIRLSNPITNEQARNIRKVLRNFAPARCS
                     LAVLDYKADPIRYNNKATYNGTYNHGSS"
     gene            29805..32084
                     /locus_tag="PXD94_000195"
     CDS             29805..32084
                     /locus_tag="PXD94_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDL6285104.1"
                     /translation="MAGVQETAQWENHVYRIEENDPVHGGENGVTNKPIKHLANRTLY
                     LRRLLTEAGQRINPKKITATSKNSNDLTGHTHEIDHASLTQKGIVQLNASIQSTSTTE
                     SATPSAVKAAYDKAATAEQKATLANEKVEAIKVGGRNLLIQSNTPHNGNAYSVRYALA
                     EAPKVGEEIVVTLWGELGSQRTGIGVYNSQGATELAKLVKIRDGVYQGKGIWRKPMSE
                     GRERTPNDTHLNVYFYPKTATDNNRIERIQLERGSIGTDWTPNEADLKAEISAIRTKL
                     IPVRGGNVATTLNAAKRSDWFREMQIPYQSGWGYYTQAFHNSGTQHNYSNLPLTGRYY
                     FIAELYHNSGYSHLRITYPSLKRTFESAINLNNEDLILDWREVVFLKDGLYQGTFKTT
                     GTLESAYRLRAARDDERYQPYLQMVDMGYDLANPPVGKNKIIGSVDYYVKNGTSESSK
                     ATMMAAAMPDKNVFWEVALWNAAQQRNVLFKGYSKTNNLSIGKTTDNERDRVQVNGTI
                     QATAPADNANNDQVPTTAWVRKMAKILADGKVSKAGDTMTGHLFIKNGEYSAVHTYNT
                     SGWFAKWEAAPLSDSHFAAIVYANDRETVINRVLIPKKNGTVALINDIADTVRAAVSR
                     SDKSRSRTFPLQTGKKYTTTGSVTIYPDGRIVQIFHLKNIKAAWFDYEASAVGDRHRV
                     VNIPLWTAMPNKIFDVDVKTVRPSNNASTYYIEAAEWLSAWQIYGNDTNKSSVNINLS
                     RFRGGKDEDMDLYVTVEGY"
     gene            32085..32687
                     /locus_tag="PXD94_000196"
     CDS             32085..32687
                     /locus_tag="PXD94_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285105.1"
                     /translation="MAYFVNVIDEQGNYELIDDEFIGLYPDLDFNTLPKLTDKQYQTY
                     LAKANGKERFVNGEFVYEQIKVDMQAVISAEKSAKLAEINQKAQAFINDLAKYNETPK
                     FERDTWLEQAKEAKAWVADPTVQTPTLELIAQMRGIPLDTLRQKAYEKAMAYQTVAAI
                     VAGQRQGYEDRLEQAETLEQIQAIEPMYQLPQGANNEQTD"
     gene            32671..32946
                     /locus_tag="PXD94_000197"
     CDS             32671..32946
                     /locus_tag="PXD94_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285106.1"
                     /translation="MSKLTKLKSWSYHVLIAIDQLFNALTGGAADETFSSRCYRGAVL
                     AENPKKRWRFWHSFLNCLFLDPKHCYAAYKAEVHRKQYPKAFRLGGI"
     gene            32946..33233
                     /locus_tag="PXD94_000198"
     CDS             32946..33233
                     /locus_tag="PXD94_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285107.1"
                     /translation="MKKQNLKLVRGDDVSFRFSIKDSTGQPLALKDLWFDLHLVSYQG
                     EVLIALSTDDGSIETAEAGQMVLHFSHQQTARAEWTSANYDLQLTHDYTRV"
     gene            33243..33407
                     /locus_tag="PXD94_000199"
     CDS             33243..33407
                     /locus_tag="PXD94_000199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285108.1"
                     /translation="MTSIIELDLTDDLIIEVQLDSTIITGDSFLANLPDLLQIYNQAK
                     SDSSKELNND"
     gene            33400..34104
                     /locus_tag="PXD94_000200"
     CDS             33400..34104
                     /locus_tag="PXD94_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285109.1"
                     /translation="MTKHKNHENHPIAITFYQLGKDVGDINRRLAVIEQREQSAQPSQ
                     PQAEAVTMPQQDAVAINNINAVLEAVEKYKTFEKYNTQWQERFEKFLSRNPNDYELSE
                     EQRQKLYHTMCQHLWQVNFTEINSKQYELPIPQQFQGKFGYIVKTSGGSTEISFELLS
                     ETHSTYLYPDSYIPEGGGDVMLKPAYIYLVIPDLVAMREPEVVGVSPLKLNILQKHLL
                     TPQKLQEQKPLNTKAN"
     gene            34273..34419
                     /locus_tag="PXD94_000201"
     CDS             34273..34419
                     /locus_tag="PXD94_000201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285110.1"
                     /translation="MNKSLVALAVAAMASKAQTKQQASKQASKQASKQASKQASKQAS
                     KQAL"
     gene            34472..35260
                     /locus_tag="PXD94_000202"
     CDS             34472..35260
                     /locus_tag="PXD94_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285111.1"
                     /translation="MFLSHFKQVLNANIEADGKDWTIIDVFGGSGLLAHTAKREKPLA
                     RVIYNDFDNYAERLNHIKETNQLRQEIYQIVDEIIPKNKRISNEIKAKIINKINDFEG
                     FKDLKCLSSWLLFSGEQVATLDELFKHDFWHCVRQSDYPEATGYLDDIEVVSESFHQL
                     LPRFHDKEKVLLILDPPYLCTRQESYKQENYFDLIDFLRLIDLTKPPYIFFSSTKSEF
                     IRFIHYMVQNRKENWQAFEGAERIVVNASASYNGKYEDNMVYKF"
     gene            complement(35297..37735)
                     /locus_tag="PXD94_000203"
     CDS             complement(35297..37735)
                     /locus_tag="PXD94_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family igA-specific metalloendopeptidase"
                     /protein_id="HDL6285112.1"
                     /translation="MKHTFKLSLLATSIAISLSPTLLASIVRGDVDYQYFRDLAENKG
                     KFFVGATNIPVIDKTGKNIGTFLQVTPTKQVEVESNISPKPNVSLSECFDNESLPGCY
                     EFLYGGGDEDSNDLPVFETVTGETMSIPMIDFSSITRQTGIATLVNPQYVISVNHNQG
                     YRDIQFGDSSSEKADDHHYNYSVVRRNNYMPDPNKERTDLGKGIEKRERNDEGEKLIL
                     DGSGNPTPRWDYHAPRLSKLVTEVAPANEIERTTENVQDFYSVFSDPFIFPMFIRAGS
                     GRQAVVNNERDRLNNGRIEVGNGPFLTGGSVLPVTNGDPSVPGSDFNSRILVAKTENN
                     TINDVFKDHGYGPLTTLGLPGDSGSALFGYDVRTKKWVVLGVYSDYFSENNTLGGDVY
                     KSYWNYHHPHYVRALEKENNAGAINANGARLTWTPSGNTSSIVGGNAPLTVNLADTSL
                     PTLQGHNGNTGDNPWNPQLQHGKTFHILGENNTLSLTENINQGAGAIHFHGNTTVEGM
                     KAGITWLGAGVDIDKDKNVIWKISNPAGDRLSKIGQGTLTINGKGENKGSVSVGDGTV
                     ILNQQADENNKKSAFSELGIVSGRPTVILNSSDQMDPNNIYFGYRGGRLDVNGNDLTF
                     RRIQNSDEGAMVVNHNTAQTANITLTGIVSPTIDQVKVQAITSPDQVSRHLDKDIFTY
                     QRFPHLPTNFIRLKKPLSVAEITAIFNARKVFIDLDFATGLIRENEYFEVLKVTDESA
                     AKQQILAEKIKAAEGTVQGFNGYFGETDPSKTNGRLNITMNSNCSEPTKLRNATTKTT
                     KENRSFTFLILMVF"
BASE COUNT        11900 a         7522 c         8803 g         9648 t
ORIGIN      
        1 atcatttaag tagtttctaa aactaacttt caaggatctc acaaaatggc aagaaataaa
       61 agagttcgag atatgagtaa tttcgaaatt cgtggtgagc tgatgaaaat cggtaagtcc
      121 ttatctcagt taggtattga gaatggatta gcaaaaacca cagttcgtaa tgcgttagat
      181 aagccatatc ccaaagggga gcaaattatt gcaaaagcga ttggtaaaac accgcaggaa
      241 atttggccct ctcgttacga ataaggagct tttatgaagg aatggttttt agccaaagaa
      301 atcgcaggat tatctggagt tccagaatta ccgaatagcg tttcaagatt ggcaaccaaa
      361 gaaggatggc aaaaacgaca aattcaaggt gtaagaggtg ttacttacga ataccacctc
      421 accagcctac ccattgaaac ccaacaacaa cttagactaa acgccgccct tgcggtaata
      481 ccgcaagcgg cagaactaca acccaaacga gacgaccccg cacttatcgc aaggctcaat
      541 aatgccaccg ataaaggcag ggataaagca aaaggcaagg cagaagcctg tatgcagttg
      601 caagcctttc tcgaccaagg gtttagttat acgcaggcag aagcgggagc ggcaaccgct
      661 aaaaatgtgt cgcaaggttc tttaaaaaat tggtactaca aagtgaaagg tcaccccgtc
      721 catttatggc aggcgatctt aatttccgaa agcggtagga gtaaaaagcc gcagctgaaa
      781 gccaagatta ctgaagaagc gtgggactgc tttttggcgg attacctccg ccctgaaaag
      841 ccggatttac gggcaagtta ccgccgaacc caagccatcg ccaagcaata cggctggcaa
      901 atagcaagtt tacagacttt ccaacgccga gtgttggcgg aagtgcctta cgaggtgatt
      961 ttgctcaaac gggaaggagc aaatgcggtt gccaagttag tgccggcact acaacgcacg
     1021 gtgaaagata ttctcgcagg agaatggatt aacggcgatg gctatcaaca caatgtgttt
     1081 gtgaaatggc acaccggcga gattgtccgc cctaaaacgt ggttttggca agatgttcgc
     1141 acccgcaaga ttttaggtta tcgaacatcg atttcggaga atactgacag catacgccac
     1201 gcattaatgg atgtgatttt taacgtaggc ataccgaaaa cgctcacgct ggataacacc
     1261 cgagcagcag cgaataaggc aatgaccggt ggtattgaaa accgctaccg ctttaaacat
     1321 accgagttag acccgaaagg cattatgccg attttaggga ttgatgtgca ttttacctca
     1381 attctttatg gtgaaggaca cgggcaagcc aagccgattg aacgtgcttt cgggcgaggc
     1441 ggaattggcg agaaaataga taaacgccct gaattaagtg gtttctatac gggcagaaat
     1501 gcacaagaaa cccctgataa ctataacggc ggtaaagacg gtgtggatta caacacattc
     1561 ttgaaagcca tagcagcagg gatagaagag tacaactctc aaaccgaacg ccataccgaa
     1621 atgtgccgag gagaattgag ttttaaccaa gtttgggagc gggattacca cccaagcaac
     1681 gtaagacaag ccagccctga acagttgcgg ttactgttct tacaggcaga aacggtcagc
     1741 attaaacgca acggcagttt tacgctgaaa gcagccggca aactttacgg cttaactaac
     1801 gtttactggg cagaaagcct gatagggata acggataaga aagtagtggc acggttcgac
     1861 cccgatgact tacacggcaa cgtgtatgtg tacaacctag aggggcaatt cttagccgaa
     1921 gcggtttgcc gagaggcgaa aggatttggt gataccagtg caagccgtga gcaaggtcgc
     1981 ttatatcaga aagtagtgaa aagtgccaaa gctcaagccg aagcccttga attactggaa
     2041 gctcacgaac tggcaagcct cgccccacaa gtggacgtgc ctgagccgat agagaagaag
     2101 gtaaaagagg tgctggtgaa agaagatttt atcgtggatt tcaacactgt gcgtaaagca
     2161 acggtggtgg aagaaaccga agagatcagt atttttgatg aagtgttact gaaacaagag
     2221 aaacaacttc gcatagtgga ataactagcg gtcagttttt gcaaaaaatt tgcaaatttt
     2281 tctaaaaaaa cgaccgcttg aaatgagatt taaacagggt ttaaacgcct ttaaggagta
     2341 aaaaatgaca ttaacagggc aagcttaccg agtgattttc aaggaactca aagagcaaca
     2401 agagagtgaa tttgaacagg ctttcaagaa agggattgcc aaaattcagc agaatcaaac
     2461 tcaggaatcc attctggtaa accgtgatat gtttcaagct gaaaagctaa cactgctttc
     2521 acaaaagttg gaagcggttg agcaattact tcgccttgaa ttaaatcagc cgaattttca
     2581 agcaaaactt gaagactacg ctgacgcatt acgaacaacc attttaagta aagtagccaa
     2641 ataagagctt tagactcttg aaggcttaac atctgagcca ctttttttac tgtcggcatt
     2701 ataccgtcaa gatcatcaat agaagttaaa tgatgaaaag aggaatggta ttcagctaaa
     2761 caaaaattga ttgtactaag tttaaacaaa gtaatgcaat ttttcatcac gatggcattt
     2821 gctcgtttta acttaaaacc agagaaggtt gcacgagcag aatcaagggc tatttgatga
     2881 atttcatcat ctgttaaaga acgaaagagc ataaaaacct ccaaacaaaa acaggacacc
     2941 attatgacat taatcgacca aattaaacaa cacatcgccg aaagcggcag ttctcagggg
     3001 aaagttgcca aagaagccgg gattaatgcc ggggcgttat cggcttacct gaatgaaaat
     3061 tacaaaggca ataatgaaga acttgaagca aagctgattg cttatttaga gcgtattgag
     3121 gcgaagaaaa gagagtttgt cgaagctccg agttttattg aaaccaaaac cgcaaaccaa
     3181 attttcggtt cgttacgatt tgctcaaaat actggcgtgc ttgccattat tcacggtgcg
     3241 agtggcgtgg gtaaaacaca agcggctaga gagtatcgta aacgctatgc gaatgtatgg
     3301 ttggttaccg caagcccttc tcgttcctca ttaagtgagg tgttatatga aattgctctt
     3361 gaacttggaa tgaacgatgc accacgccgc aaagggacat tggctcgttt aattgttcgc
     3421 aaaatgaaag gcacagcagg tttattaatt attgatgaag cagaccattt accttacgaa
     3481 gccttagaag aattaaggct gttacaagaa gaagtagtga atgaaagtga catcggagct
     3541 ggtgttggct tagtgctgat tggcaatgac aaagtctaca cccgaatgaa aggcggaatt
     3601 aaccctgctc acgaatatgg gcgactttgg tcacgcagtg ccaaacgcac cagtattcag
     3661 aaaaccaaaa aagaagatac ccaagcggta gccaaagcgt gggggttgag tgaaaaccaa
     3721 gaagcactca aggtaatgca agccattact gaaactggtg gcggattacg gattctgaca
     3781 cacaccttac gattagcagc gatggtagca aaagaccgaa tgattccctt aactgccgat
     3841 ttaattcagc tcgcacgtaa agatttactc tcaacaggag attaacaatg agaaccccaa
     3901 aacccaaaca cagttttaac cgcaccaacc aagcagcgtg ccgtttttta aaacaaaccc
     3961 aaaaggcaat tatccgctta aacagtcttg gctttacggt gctgaatatt gattttaccc
     4021 gtattaagcc ccgcattgag gtggaaatcg gcaataacaa gcacattgct caaggcttga
     4081 tttatgaggg taaagcctac cgctacagct tcggtaaaag cgaagattta ggcagatggg
     4141 aaggctacta caccatgctg gaaggtatca gagtgtggtg gcgacaagca gcaattcact
     4201 aggagaaaaa cagatgaaaa ccttagcact attaaccgcc cttttagggc ttgtagggtg
     4261 tgatttagtc caaagcaaac aagctaaaaa ttacatcggc aacaatttaa ctgaaatctg
     4321 tatagataac gtggtgtacc tgatttattc cggtgataaa aaaggcggaa ttaccccgaa
     4381 agtaaataaa gatttttacc cttatacctg tacaaacaaa gaggaaagac aaaatggcaa
     4441 aacccgatga aacacccatt acaaaagcag aatgtcaatc tcagcttgca gagttggggg
     4501 tgcaatataa aaaactgcca atggcaatta caagacatat ttgtaatgcc acaaccaata
     4561 tacacggcaa agttataaag gtcagtgtgg tagagagagt tggatatggc gtccagatta
     4621 ccgcacaagg taacgagaaa tcttgcttag taacctatga agcaatgtta ggtatggcag
     4681 aagcaatggg attatttgat gaagttaagg agcaaaataa tgactaaaaa aacaaccaga
     4741 gtaaaaagtg caacccaaag tgcgatttat caaagccgag atgaagtgca agtggctata
     4801 aaaatcattg gtgataaaca gcgtgaattg caacgtttag ctacggcaat gaatgatgaa
     4861 ttagctgcta ttagtgccag ttatgctcaa cataaccgct accaacatta tgccgagaaa
     4921 gcggctgaag cagaacgcaa aggcaactat aaggaagctc aagaccattg ggaagttgca
     4981 aaattatcag caaaaaagac cgctaaccga gactgggcgg aacaacgagc ggaattttgt
     5041 aaacgtatgc acaataaacc gttttagggg gaagtgatga cagaaaccgt aaaagcacaa
     5101 cttaacagcc aattaaatga ggcaattatc cagttgatac aagctcaaaa gtatttaaac
     5161 caagatgatg ccattcgcag tggagtgtat atcggtacgg tgcaggattt attgccaaag
     5221 gtgcatttaa agttattaac ggcaaatcgt aaacattgat agagatgagg taaaaatggc
     5281 tattacagaa gaacaatgga aagaaattaa gcaaaagcta gatagtatca tagggagagt
     5341 taaatttaga tacaaagagc atttactcac ggtagatgtt gtacaaatta agcgatcttt
     5401 aaaattggct gtgtatgttg atggggaaat tgatggagct tggacaaaag aaggacacga
     5461 aattcgccct tatttagaag aagtttggta tcgcaaagaa cgcccttttt ttaatgctaa
     5521 ggagaagaaa gagtatagag gtttaatgag caaaaaaaaa ctgaatgaaa aaatagtaac
     5581 ctactcacct atgtttccat caccaactgc actgactcgt caatataaga aactagatgg
     5641 cttagagttg attgaagtga tttaaaaccc atttacagcc catttaaccc cgagttattt
     5701 aaaggacaac aaaatgaata aaaacgaatt ttttaagaaa taccacctag agggttcact
     5761 agctattttg gatttggatg caaatgacga tcaaagccaa ttctgttacc aagcattgtt
     5821 ggagtataga gaactcccgt tagacacatt tcttgcagag tactcagatg atgaggatat
     5881 gacagagtca gagtttattg agttaatgct tgatgaatta gagtggatta tccgcagagc
     5941 taagaatgct aaacggtttg ttaaaaaacg acaaaaaacg atgagggagg aaattatgta
     6001 acagctcaac ccaaccaacg ccacatcatc cgtctagctg agaaacaggt gtggcgtttg
     6061 ttttaagtgg gcaatcaaaa gcgataacgt ttttaattgt ttatttatcg gagtaacaaa
     6121 tgacagacaa agccaagcta atccagctta tccatattgc caagtcgcaa ctagcaatgg
     6181 acgatttaag ctaccgtgaa atgctgaaac ggctcaccaa taaaaccagc tcgaccaaat
     6241 gcacggtggt cgagttgcac aaagtcttac acgaactgca aaccaaagga gcaaaagtaa
     6301 aatattttgc caaacgcagc aaaaaaccga ccgcttacag cccggcaaca ggggaaacag
     6361 cggtaaaaag ccaaattgct cataaaatcc gagcggtatg gataacaatg ggcaaacacg
     6421 gatttttaaa cgacccaagc gaaaaagcat taaatgctta tatgcgtaag gttatcaata
     6481 ataaaactag accaacaata ttattaaatg tctctgcatt agaggctgga gatgcaagtt
     6541 atatgcttga aatcctcaaa aaatggcata aacgtgtgat gattaaagcc ctaagcaaaa
     6601 gttgcacagt tgatccaaag ataagctacg atgaattagt ggagtggtac aatgaagtta
     6661 tgtagatgcc cgatttgcca cagtgatata cacctagaag ctctcgtaga agatgaagcc
     6721 gggcgtgaac tactcggcaa aatcagccaa ttaacacacg gtgttgctca accgatggtt
     6781 gcttatttag gcttatttaa accgcaaaaa agtaacctta ataactcaag agccttgaaa
     6841 atactcaatg atgtattagc actctaccct tgctcattgc tactggctca agccctatct
     6901 gaaacggtag taagcatacg caaaaaacgt cagcaagcaa tggaaaacgg gcagaaaatt
     6961 gaaccgctac cgaaccataa ctatttaaaa tcggtgtatg aaacgcaaaa agtccatttt
     7021 gctgtggtga gaaccggcaa aaccgaacgc gaaacaatga aagccgaact ggaaaaaaca
     7081 cgcaatgcga ttgcctatgt gcaacggttt gttgatttag gacgtgaaga ggttgttaaa
     7141 aatagccctg agtatcagat ttggttagct cataaaccca aataacacaa gccaccaaaa
     7201 ggtggctttt taactttttg aaagtgcgat aaaacctgat ttaaacaggc tttaaacggg
     7261 ttttaagatt gagtttaaaa aaatatttca caaaataaga atggtttttt atacaataac
     7321 cgaaacggtt tttggatagg aaatagacaa tggaattagt ggatattttt gaagaaaaag
     7381 ccccggaagt gttaaccgat ttagccaaac atattgaagt cgcattagaa gaaaaatgcc
     7441 aatttagccc cgaaaaggca aagcaaatcg gtattgatat tgcccaacaa atggctcaaa
     7501 actggggtgg cgaggtaatt tatattcctc gcaatttgtt gatggctctt aatgagcgag
     7561 atatgaaaat ttacgaagag tttaacggta acaatcaccg tgaattggct cgtaagtaca
     7621 atgtatcaat gcagtgggta tataaaattg tgaagaaagt ccataaggac gaagttgccc
     7681 gcaggcagat gagtatgttt gagatgtaaa tcattttttc ccagccctga tcattttcag
     7741 ggctttttgt gtattaaaag aacgcagtct ttaaacccgt ttaaaatcaa tttttcagca
     7801 aattctttaa actcctttta aagcaatttt aataggagtg tgctatgtct caaaatctta
     7861 cctttcgcca aatttttgac cgtttaatcg gacacgaggg cggctatgtc aatcacccac
     7921 aagacccggg cggagaaacc cactggggaa ttaccaaacg taccgcggtt gctaatggtt
     7981 atatgtatgc aatgaagaat atgacccgtg agcaagccta ccaaatctac gaaaaagcct
     8041 tctggcaacg ttatcgttgt gcagagctta agccagcagt tgcttatcaa tttttcgacg
     8101 cagtggttaa tcacggtttt ggtaatgcta gtcgtatgtt acagcgtgca gtaggcgtag
     8161 cggatgatgg cattattggc aatattaccc tgcaggcaat taatgcaaaa tctgaaagcg
     8221 atctcttaat gctatttaat gccgaacgtc tgaagtttta cacaaaatta cgcaacttcc
     8281 ccacttttgg taagggttgg gtcaatcgtg tggcaggcaa tttagaatac gcagcacaag
     8341 ataatgaggt ttaaaaatgg caaaactatt taaagaacta ctctcaacca atggcacatt
     8401 atccaccact agcaccgtgc agttttttgg cacattgtgt gtatttggtt taatggttta
     8461 tgcagtaatt acccaacaac cttatgccga aagcctatta aataatgtgt tgatttatct
     8521 ttttggggca actaccgcta agggtgtagt aaccagctat caagccaaaa ttaaaggggg
     8581 tatccataaa caacaaagta catttaaaga ggaggaggga tgttaatgca acaaatttta
     8641 ggcgtgattg gcgtcatcgc agcaatattg tcttatgctt tgtttaaaag ctggcaacta
     8701 aaacgtgagc gtaaaaagaa tgccatatta caagctgaaa aacagcaaca agcggttgaa
     8761 attgagcaaa aaaaagcaga ggtaaaatat gtgtatatat caaagaaaaa caatgaaaaa
     8821 gttactcgca gctctgctag cgatattgat gacaagttgc agcaacacgg ttacttccgt
     8881 gacaacaaca ggttgcacgg cgttcagtct gatttatccg aaccgtgcgg atacgctgga
     8941 aacgaaacgt cagattcttg ctcataatct gacttttgag gaaatttgcc aaaatgaaat
     9001 acattaaaaa attacgtaaa cgctggcaaa tttggcgttt tttaaaaaat catcctgagg
     9061 ttgcacaacg ccgagattta ttagcgattg ccattaaaca gggcgtagaa aaccctgtag
     9121 ggcgtgcaag acggagggag ccataatgga tttatttaca gtcttttggg agtctatccg
     9181 tgctaacttt ggttttattt ctgcgttact caccattgtt gttacggcat tttggctcaa
     9241 gctagatagc aaatacgcca agaaaaatga tcttggtgaa ttgctggaaa ttgccaaaag
     9301 ccacgataac cgcttaacca ccttagaaac caaggtagaa aacctgccaa gtgcggtgga
     9361 cgtggaaaag ctgaaaacct tagtcactga tgtaaaaggc gataccaaag ccacctcaaa
     9421 gcaggtggat agtatcagcc accaactcgg cttgttatta gaagcaaaat taaaggaatg
     9481 atatgtcgtt aagagattta ctcactcaag accaacggct ggtgatttta cgctcattag
     9541 cggaagccgg ctatgatgcc aatgagtcaa ttattagtga ttgcctagat ttatacggtc
     9601 acgatattag ccgtgattta gttcgcaccc atttaagttg gcttgaagag caaggcttac
     9661 ttaccttaga acgtttgcga gatggctata tggtggcaag cattacccag cgtggcttag
     9721 atgttgctca agggcgtgtt gtggtaagtg gcgttaaacg ccctcgccct aaaatttaaa
     9781 cggtatttaa ggagcgttta aatgagcgag aaaaatactc gtggtcgagc atcaaaagtc
     9841 gatttactgc cgccgaatat taaaacccaa ctggcgatga tgttgcgtga taagcagtac
     9901 tctcagtcac aaattttaga agaaattaat gacttaattc gggattgtgg cttgcctgat
     9961 gaaatgctgt taagcaaaac cggattaaac cgctacgcaa gccgtatgga acgcttaggg
    10021 gcaaaaatcc gtaatgcccg ggaagttgcc gaaatttgga ctaagcaatt cggcgaaatg
    10081 ccacaaaccg atattggcaa aaccctaatg gaaatggtca agcaaattgc ctttgaaacc
    10141 tcattgaaat taggcgaaca agaaggcggt attgagccaa aacagcttgc tttgctctcg
    10201 tctgctattc aacgtttaga acaagccgaa agtttgagtt ttaaacggga acaggcaatc
    10261 cgtaaagagg tcgcacaaca agccgctgaa accgctgaaa aagtggtggt gcaagcgggt
    10321 ttatccgctg aaaccgtccg tacgattaaa gagcaaattt taggaattgc ataatggcac
    10381 tactcaataa cagaccgctg aatgaattag cccctgaatg ccgttcgttt ttagagagca
    10441 ttcacgcttt taatcctaac gaactgttat tgggctatca aaagcggtgg attgcggacg
    10501 acagccaact caaaattgcc gaaaaaaccc gccgttgcgg tttaacgtgg gcagaagcag
    10561 cagataacgc cttaatcgca agcacccgca aatcagacgg tggctctgat gtattttaca
    10621 ttggctcaaa taaagagatg gcacgtgaat atatcgatgc tgtcgcaatg tgggcaaaag
    10681 cctttaatcg tgtggctgga gaaatccaag aagagatctt tgaagacgaa gataaagaca
    10741 tcttaaccta tgtaatctac ttctcatcgg gttttaaggt caaggcatta tccagtaatc
    10801 cgaaaaacct gcgtggtatg cagggcgtgg tggtgattga tgaggcggca ttccacgaat
    10861 acttagcgga agtcttgaag gcggcgttag ctctcacaat gtggggggcg aaagtccgta
    10921 ttatttcgac ccacaacggc tcggaaaatc tgtttaatga actgattacc gatagccgtg
    10981 ccggccgcaa acgctactct gtgcatacga ttacgattga agatgcctgc cgtgatgggc
    11041 tatatcaacg tatctgccaa gtagtgacga aaaaaacgtg gtcaccggaa gcagaaaaag
    11101 agtggattga aaacctactc aacgacaccg ccagcgaaga agacgcattg gaagaatact
    11161 tctgcgtacc gaagttcggc tcaggcttat ggctctctcg tgctttgatt gaacggcaga
    11221 tgagcgaagc cacgcctgtt atccgcttta cggcaaaaga tggtttcagc cttgtggctg
    11281 aaccgacacg ctatgcggag attgccgaat ggtgtgaatt agaacttgag ccgatattgg
    11341 caacccttaa ccctgatttg ttgcatttta tgggcgaaga ctttgcacgc agcggcgata
    11401 tgacctctct ggtggtgctt gctcagcaac aaaaccttgt tcgtaaagtc gcctttattg
    11461 ttgagcttgg caatatgccc tacaagcaac aagagcaaat cgtgatgttt atgcttaaac
    11521 gcttaccgca ttttgccggt ggggcttttg acgcccgagg caatggtggc tatttagccg
    11581 aagctgcacg agatgaattt agcaccttga ttgactgcgt gcagttatcg gaaaaatggt
    11641 atcgggaaca caccgccccc tttaaagcgg cattggaaga cggcgagctg gaagctattc
    11701 caaaagatgc ggatattttg gctgatttgc gttcatttca ggtggtgaaa ggcgtaccac
    11761 gcataccgga taaacgcact aaaagtgcag atgggaaaag caaacgacac ggtgataccg
    11821 caatttcttt gctgttggct cattatgcca gccgacaatt agtgcaaatg ccagttgagc
    11881 cggtatcacg ccgtccacgc catagccaac aattaacaga aggatattaa caaatgagat
    11941 atgtaccact ttgtttaatg atgtgtgcct cattctacct atttattaac aacattgacg
    12001 gctggggttg gggaattctt ttatctttta ttttagcgag tgcagcttat ggcaaagaag
    12061 aataatttaa ttagtgaaat tgccacccgt gccagaagta ttgattactg ggcatttggc
    12121 tattatctgc cgaaccctga cccgatttta aaacgaatgg ggaaagatat ttccgtttac
    12181 cgtgagttgt tatcagacgg gcaagttcgc tcaggcgtac gccgtcgaaa agcggcgatt
    12241 aaaggcttag agtggcgaat taccaccact aacaatgaga aagtggacga gcagatttat
    12301 caaatattta accgcttgcc gatgaataac ctgattaccg aaatgctcaa tgcagcactc
    12361 tatggctacc atatcagcga ggttatttgg gcaaaagagg gtgagttatt tgtgccaaca
    12421 gaaatcattg gtaaaaagcc tgaatggttt atgtttgacg acgaaaacct actgcgtttc
    12481 cgtaccaaag aaaactgggc ggacggcgaa ttattaccag atgataagtt cttgcttact
    12541 acgcaagaag ccacccaaga taatccctac ggtttaggcg atttatccct ctgcttttgg
    12601 gcggcgacct ttaaaaaagg cggtttcaaa tactggttgg aatttaccga aaaatacggc
    12661 tcaccgtggt tgattggcaa acaccctcgc caagctcaca atacagataa agagcgacta
    12721 gctgatgccc tagagggaat gattagctca gcgattgcgg ttatccctga agatagttcg
    12781 gtcgaaatca aagatgcagc gggcaaatct gccagcaccg atgcctttga aaagttcctc
    12841 aatttttgca aggcagagat caatatcgcc ctacttgggc aaaaccaaac caccgagcaa
    12901 gaaagcaacc gtgccagtgc acaagtaggc ttggaagtta tggaagatat tcgctcggac
    12961 gaccaagcga tgattgaagc caccttcaac accttgttgc agtggattgt gaaatataac
    13021 ttcaatgtgg aacaactgcc gaagtttgaa ttttacgagc aagaagccat caacaccgac
    13081 caagttgaac gagacagtaa actccacgca atgggcgtgc gtttttccaa agcctacttt
    13141 gagcgtgaat atggctttga ggcgggtgat attgaggtgc aagcggtcga aaaagcggaa
    13201 aaatctgcaa ataccacaga atttagcgaa ggacaacgcc gaacccaagt gccaatgcct
    13261 gtgccaattt tggaacagct agaacacgag ggagaagcaa tggtggacga ttggctacaa
    13321 ggggtgagag ataaactcgc tcaagcagat agtctggagg atttccaaag tcagctagat
    13381 agccttatcc ctgaattaag ctttgccgaa tatggcgagc tgttggcgtt aggctcaacg
    13441 gtggcagagc ttgcaggcag acaatcggta aacgatgaaa ttaaggcaaa acgcaatgcg
    13501 taaatttacc tttaaacagc aagcccaata ttttgagaaa aaactcaatc tccgcacgaa
    13561 tagctacctt gatattctgg gcgaagagca cgactatttt ttcgtggtgg ctggggcgaa
    13621 tcgtaatgaa gtgttgagtg agtttcgcaa ggcggtggat gaggctattc acaacggcga
    13681 aaccttagag aactttcgca agcggtttga tgaggttgtg gcaaaaacag gctgggagta
    13741 caacggcgga cgaaattggc gaacacggat tatttacgat accaatgttt acggggcgta
    13801 taaccgtgga cgtttgcaac aacatttaga gcttgcggag gatatgcctt attgggaata
    13861 tcaacacaac gacaacgccc accctcgccc acagcacgtg gcgtgggacg gcttggtgct
    13921 gcgttatgac gatccgtttt ggaaatatca ctatccaata aaagcctacg gttgccactg
    13981 caccgtgcaa gcgttagatg attttgacct caagcacgat ggtaaaaccg tcagcccacc
    14041 acctgaaatt gagtttgaac aaaaaacggt aggcgtgcgg acaggcaatt cacgagtcgt
    14101 aactgtgccg aaaggggttg atgtgggttt tacaccgcat aattttgata atctcaaagc
    14161 cgggagagca gcaaatgtag ataagttgct aatgcaaaaa atggttaccg ccgatcctat
    14221 atttgcctcc aaagcagtca gtgatttatt ggcaaaagag ccggttgcaa agttacataa
    14281 ccaagccttt aaaaattggg taaatgatgt tgccaatgct aaaggaagtg cattatccca
    14341 agtagagaat atgcagttgt taggggtact acctgttgcg gtgattgaac ggttacagca
    14401 gatcaattta gctccgcaat ctgcaattat tgcaataaca aaaggcgata tggtacacgc
    14461 tctacgagag ctaaaacaaa ataccggtat tgccgtaccg ttggaatttt gggaaaagtt
    14521 gcctgcaaag ttacaatacc caaatgccat cttgttggaa aaacaacaag cagaaactac
    14581 attgctcttt atctatgacg tagctcaaac caaaattgcc gtaaaagtca attatgatgc
    14641 aactttacaa gatagacacg gagcaaaagg acggcagaaa gtcttactga atcgagcggt
    14701 tacaggtagt ttattccaag atacaactgg attgaaaagt tcaaagtatg aagtgctatt
    14761 gggagaaatg tagcggcgaa ggactcgaac cttataatgt aggttaaacc tacaaccttt
    14821 accaattagg aaactaccgc tacattgtgt aaattcacta tacccctaaa ttatttttta
    14881 atcaatagga gaaacaaaat ggcagaaaaa caactttcct ttaaagtagc cgaaatagtg
    14941 ccaatgtcag ctattaacac tatgcggcac ttaattagtt tggcattaca agcgaattat
    15001 gagagagaaa aatctcgtgc ttgataattt caatggcttg tagttcggga actatattat
    15061 ctgaaacaag ccattgtttt gttgatgtca gacatttagc gatgaaatcc ataattaaac
    15121 cattagcagt agctcgttgt tccggagtaa gttgtaacca cgacgcctct gtttgttggt
    15181 ctaatgtata aagaaacata acggcttctt tttctgataa tgggtcaagt ggtaattttt
    15241 cagcttttgt gactaaccat tgacgggaaa gcaataaatc ataaaccagt tctattgctt
    15301 gttctgcatt taccatcata ttctaattcc ttaatatatc agggcaacat tacccaatct
    15361 atattttact gagatttgct taatgatcaa aatcaccctc aacaacaccc aagcggtcaa
    15421 atcgttacaa caaattgcaa atcagctgga acaacctcgc cgactgtatg gcattttagg
    15481 ggaaacccta aagaaaattc acactgcacg ctttaaagcg gaacaagacc ccgatggcaa
    15541 caattggaaa ccgttagcca gtagcacgct tgctttaaaa cgtaaacgag gtaagtccac
    15601 taaaatctta cggcaagacg gctaccttgc cgataaaacg gcttacaatg ttacaagtga
    15661 taatgttgag tttggctcgc aggaagtgta tgcacggttg catcaattcg gcggtaaagc
    15721 cggacgaggc aaaaaagtga ctgtaccggc tcgcccgtgg ttaggcgtag gcgataaaga
    15781 tgagcagtta cttttacgca aagctgagtg gcatttaggg caaattatcg gtcgtatggg
    15841 aaaataaccg cttaaaatca aatttaacgc ataaaaacgc ctctgtgcgt ttttattttt
    15901 tattcgcctg attattcgac tcaaaaaatt taaacgctgt taaacgcatt taaacggtat
    15961 ttaaacggta ttgccgattg tgataaatcc tctttattaa aacaccctcg aaaaatcttt
    16021 aaaccagttt aaaagctaat ttctctccaa atccttatta ttgccccatc acggaggcaa
    16081 tatgacccaa attgaaattt ttaaagccgg taagcgttat gacgccaacg gcacgttaat
    16141 tgacattacc cctgaaatgt tgcaacagac cgttgctgcc tataaccccg aatttcacga
    16201 agctccgctg gtgatcggac acccgaaatc caacaaccct gcgtgggggt gggtaaaagc
    16261 cctttccctt gagggcgatg tactaaaagc agaagtagat cagctagacg ctgagttttc
    16321 agaaatggtg gcaagcggca aattcaaaaa agtctcggct gctttttact tacctgatag
    16381 cccgaataac ccacataaag gcgtgttatc gctacgccac gtggggtttt taggggcaat
    16441 gccaccggcg gttaaaggct taaagcaggt ggaatttgca gaagatgatg attttgtcgt
    16501 cttttctgaa tggggggttg caagtctatt tagtcgtttt cgtgattggt tggtgagtga
    16561 gttcgggctt gaaaaagccg ataaagctct tcccccacac gaaattgatt ggctgaaaga
    16621 agaggcaatg cgaagccaaa ttagcaaaca ggtacacgcc gaacaggcaa cacctgaacc
    16681 gatttttaat gaacccgcac aaccagaagg agaacctgaa atgagtgcag aagagaagga
    16741 agagcttgaa cgcttaaaag cggaaaacga gcagttgaaa gccgaaaaag cccaagccga
    16801 agcagagaaa atggaagcag agctggaagc tgaaaaagct ggtaatgcag agttttgtga
    16861 aggtttagtc aagcaaggca aacttgcccc ggtggcaaaa gcggcgtttg tgcaggcttt
    16921 aaataacctt tcagtattaa aagccggtcg tgagccggaa tttaatgagg gcgaagatgt
    16981 ggtttcacag tttaagtctg caatgtcgca atcaccacag attatccaat ttggagaata
    17041 tgccacagca gataaagcca acccacaaga agcggaagca gtggaatatg ctgaaggcac
    17101 agatccagtt tcgattgaag ccgaccaaaa agtgcgagct tatatgaaag cccacaatgt
    17161 aggctacgct gcggcattta atgcgattta tcgctaatgt ttaacccaca actgaaaagg
    17221 aatactaatg acaactcata acttacaaaa actgcgtgta caagaccccg ttttaaccga
    17281 gctggcacaa ggctatcaca ataacgagct ggttgccgag accttaatgc cgactgtcga
    17341 aatcgaaaag gaagcaggca aaatcccaac tttcgggcgt ttagctttcc gattgccttc
    17401 taccgtgcgt aacttgcgtg gttcatctaa ccgtttagat ccagaagata ttggggcgat
    17461 tgatgtggcg ttagaagaac acgatgtgga atatgccatt gattatcgtg aagagaacga
    17521 agccattttt tctttacgtc aatttgcctt aaataccacc caagatgtga ttgcgttagg
    17581 tcgtgaaaaa gcggttgcaa ccctcgcctt agatgaaagc cgttatgaaa cagacaataa
    17641 agtcacgctg tccggcacca gtcaattcag ccataaagat gctgatgtgt ttaaggtatt
    17701 tgataccgct attcgtgccg tgaagcgggc tatcggtcgt aaaccgaatg tgtgtgtgat
    17761 ttccggtgat gtgtgggcag cgttaaaaga acatccggca gtggttgaaa agctcaaata
    17821 cagtcaagta gcgattgtta cccctgaggt gtttgccaaa ctgatcggca ttgagaccgt
    17881 aaaaatcggt gaagcggtgt atgaagaaag cggtcagctc aaagatattt ggagcaaaga
    17941 tattgtggtg gcgtatgttg ctcctcgttc gaccggaggc aaaggcacgg tttacgagcc
    18001 gtcttatggt tacacggttc gccgtcaaaa aggcttattt gttgatactt ataaagagag
    18061 tggcggcaaa attgaagtgg tacgcacgac cgatattcac aaacctcatt tagtgggtgc
    18121 ggcagcaggc tatttaatta aagctgccgt ataagccaca taccataggg tgcgtttact
    18181 aacgcaccat tatctcaaac acattacata gaggaaaaaa cgatggctaa atttattgtt
    18241 ttacataccc caattttaca cgatggcaaa cgctatgagg tgggggcaga aattgaactc
    18301 accgctgaac aagctgagaa caatgcgatc aacttaaaag cggtagaagc tgttccaaaa
    18361 ccacctaaaa atgcctcaaa acctgaagat gagccaaaac ccgaaggtga acctaagcct
    18421 gaagatgcag tgacggaaga tgatgtgaag ctggctgaag caaaaattgc agaagcagag
    18481 caaccaaaag ggaataaaaa ataatgtatg tgcagattga tgagctactc acggcgttta
    18541 gtcgcaagat tgtggtgcag ctcagtaatg atgaaccgac agcgaccgag cctgatttaa
    18601 agatgcttga aacggcagtt aaagtggcaa atgagcgaat tgatgcagcg ttacgctctc
    18661 gctatacctt gccgctggct gatgtgccta cgcttattaa tcagcacgca ttaacccttg
    18721 cccgttattg gctttatgcc cgccgtcctg aaaccaaaat gccggacacc gtgaaggaaa
    18781 cctaccaaca agcggtgaaa gagttggaac agattgcaac aggcaaactg catttaggca
    18841 ttgctgaaag cagtcaaatt ttagaagacg atgttttagc cgataacagt gaatatgaag
    18901 ttcgggcgaa cgctcgcatt gataccagtc gttattaaag attatgtcag ccacacaacc
    18961 gattttagaa gatatttgcc aacacttgcg gcaagcattg cccgagtggg aggtggaact
    19021 cttcccgaat gacccaagca cctatcatct ctcgcacatt aacggtgcgg tgttgattag
    19081 ctatttggca agcaaatttg agaaacctcg cactacacaa gcggtgctgc aggttcgcca
    19141 cgttcagatt gctttaacgg tgctgacccg tgatttgcat aatgatgtcg gggcgttgga
    19201 tttgctggat aaattgcggt tattggttgt ggggtttaag cctcaaaact gcggtgagtg
    19261 ttggttgatt gatgaatttt ttaatggctc aagcgaagaa accgggcttt ggcaatatca
    19321 gttgattttg caaaccgaaa ctcagcaggt gcaacaccaa gcggtcgaaa atctgccaaa
    19381 atttgtaacc gctcatcttc gccgtcaaga ccagcctggt tcgcccggac ttaaaactta
    19441 aatcgtaggg tgcgtttgct aacgcaccat caacatcacc tgattttacg gtgcgttggc
    19501 aaacgcaccc tacaaggaga atattatgac gtttcaccac ggttctgaaa ccgaacgagt
    19561 aaacggtggc tctgtgccgg tatcggtggt tgatagtgcc attatcggca ttgtcggtac
    19621 tgccccaatc ggcgcagtta atgaattgac cgtctgttta accaaaaaag attttgccaa
    19681 attcggcacg attttaaacc aaggcttcac cttacctgat gcctttgata ttttagctcg
    19741 ttacgcagcg ggtaaagtgt atgtggtgaa tgtgcttgac ccttcccgcc accgtactac
    19801 cgtgaccgat gaagtcctca cactggatcg caatacgctg accgctcaaa cagcgaaaac
    19861 cggcattatt gcggtgacga gagtgaaaga cggtgcaaac gtgctgaatg ctgatcggta
    19921 ttcggtcgat ctgcaaaccg gcgtggtgac ctttgtctct gcaccgtctg ccccgaaaat
    19981 tagctatatc tatgctgacc cgagcaaagt gaccgaagag gacattaaag gcggggtgga
    20041 tagcttaacc ggtaaacgca aaggttttga actattgcgt gatggtttta acctttacgg
    20101 tgcggatgcc aaaatcttaa tctgtccgga atttgataaa accgcaagct gtgccgctgc
    20161 ccttgccacc cttgccgacc aactgcacgc taaggcgtat attcaactgc cgaaagggac
    20221 gagcttatca aaagctattc aaggacgtgg cccggcaggg acgattaacg cctcggcaag
    20281 caatgagaac gtacgccatt tcttcccgta tgcgttgggg tcaagcaata ctcttgaaag
    20341 ccttgcgacc cacgccgccg gtttgcgtat gttgattgat gtggagaagg gctactggtt
    20401 cagcacttcc aataaagagc tgaaaggggt gatcgggttg gaaattccgc tgacggctcg
    20461 ggtggacgat aagcaaagcg aaactaatct gctcaatgcg gtagggatta ccaccatttt
    20521 caatagtttt ggcacaggct tccgcttatg gggcaaccgt tcgtctaact tcccgactgt
    20581 gacccatatc agcaactttg aagtcgcctc tcgcacaggc gatattattg atgaaagcat
    20641 tcgccaagcc gaattgcagt ttattgattt accgattgat gatgcgttgc tggatagctt
    20701 ccttgaaacc atcgacactt tcctgcgtac gcagaaatcg ttggtaggtt attcggtgag
    20761 cttagactat gaatatgact tggttgatgc attcagccgt gggcaagtcc cgattgtgta
    20821 tgaatacact cctaaaattc cgggtgaacg tatcagcaat aaatctgtga tgacccgtaa
    20881 atacttggca aacttggtgt cgcaacgata aggggaaata aatgagtatt gcaattaacc
    20941 aaattgtaaa cggaaatgtt tacattaacg gcaacagcca aatggggcga gtgaatgagg
    21001 tcaaaatccc cgatattgag tttgaattta tcgaacacaa aggccttgga atgcacggtg
    21061 tgattaaatt acctgccggc acgaatgcga ttgaagcaga aatcacttgg gacagttttt
    21121 atccggaagt gcgtgcggta atgcttaatc cgtataaata cactcagttg caaatccgct
    21181 ctaacctgca agtgtttaac tcgcagggct tggcagccga agagccgatg gtcaccatta
    21241 tgaatgtgtc tgccggtaaa atcggcggca cagggcataa aaacaaagaa aatgccgaat
    21301 ttgccgacac gttaaatgta cattcaatta aacagaccgt tgccggtcgt gagctgttat
    21361 ttattgatgt gctggcgaat atctaccgtg tgaacggggt ggatgtgttg caaaaattcc
    21421 gcaccaatat cggttagtaa aatctttaaa gccgtttaaa atcaatttaa acaccgttta
    21481 agtaaactcc tttgtgaagt taaacaacaa ccactcacaa aggagttttt ttatggctaa
    21541 cgcagctcaa caagtattaa ccaatcttcg tgtacaaacc acttataaat tgcaataccc
    21601 tatcgcttta ccagatggta gagttattga aaccttagat gtacgtcgtc caaaaggtaa
    21661 agattttcgt ttgcttgatg aaaaagggtt tgaccctgaa aaagatggcg taaaaattct
    21721 acgcttctat attcaacagc tcaccacact tgtgccggaa gatattgatg aactcgatgc
    21781 tgccgatatt aagggcttaa ataacctatt ggaagatatg cttattgagg gaaagtccga
    21841 caaataagtg aactagatac cgtttatgct gatctcgctt ggtggtttaa gttttcacca
    21901 agcgagcttt tagaattgga tttagtggat attccgaaat gggtagaaca gatgaacagg
    21961 caaataaaag ccggttatgg gcagatttta cggtagagcc aaataaaggg ttcggtggct
    22021 aaagtaagta aaatggcgac gccccaagct agatacacac caacccacgc caagattgtc
    22081 aaaatcaatc cataaatgag tacaaaacta acgccctcgc ttaggctttg tatagattga
    22141 atcgcatcta accagtagaa gatactccaa ccgactaatg ctaccgctaa caaccattta
    22201 gcggtgcatt ctgttcttgt tacacaagcc tcgaaaccag cccactcagt aattcgatta
    22261 tttaatttag tcagcataca ccacctcctt aaactttccc tattttacgc taaggattta
    22321 accaatggca aataatttag ctatcggaat ggtaattggt gcaagcctat catcaggttt
    22381 tcacggtgct ttttcaggag caaagaaaaa gctagaacaa ttagaacagg ctgtcgctaa
    22441 aaatcagcaa agccattcta aattaggttc tgagcttact gcattgcgta gtaaacaagc
    22501 tactctctat gcagaaatga gccgagccag tagtaaaggt ggtgctgggc ttgctcttat
    22561 gcaacaggaa tataaaaaaa ttggcaaaga aattgccaat attagccgag agcaacgcaa
    22621 atggagtaca gaattagaaa aatctattgc taagcaaact aaattacgca atgcaatgga
    22681 acgtcataca caagctaaag aaaaccgaga cgagcttaaa gggaaaataa ttggaacggt
    22741 agcttctgcg gcaatgggtg tcggcgtgat gaaaacctat atggagcaag aagaagctgc
    22801 gaataatctt aaaatttcaa tgctgaaagc agatggttct ttcgggaaat ttgaagagat
    22861 tggcaagatt gctgacacat tagggacaga tttaccggga acaaaaaaag atttctacaa
    22921 gctcgcaatg gcattaaaga aacaaggtgt ttctgatgat gtgctgaccg gaggggcatt
    22981 aaaaacttct gccgaactga atgtcttgct tgatatggat caggaaagtg gtggagagtt
    23041 tttagcgaag tttatggaat ctcaccggct caatgaaaat gagttaccac aagcagccga
    23101 ttatctgcaa cgagcaatgt ttgccggcgg tttaagtaaa gagcagatgt atgaatcgat
    23161 gaaatactat gctccaaaac tgaactcaat gaaacttaca ggggctgaga ataccgaaaa
    23221 agtgctggcg attgaagcaa tggcaggtca gcaaggctta gagggttcaa cctttggtac
    23281 gggcttaaat atgatgttaa gccgtatgaa taaaggaccg gaaatgctca aggcagcagc
    23341 caaaggaatg aaagccgaag ctcgagagat gatggaggct gtcggagtag aatttaattt
    23401 ctgggataaa aacggctcat tcaaaggcat tgatggaatg ctggctgaaa tgacgaaatt
    23461 tgaaaaaatc cgcaaaaaat tcggtgacaa aggtgtaggt ttagttgcag aagagctgtt
    23521 tggcattgaa ggtgggcgtc ttgctgatat tttggctatg aaaggtcaaa aaggcttaga
    23581 tgaaatgctg agtaaaatgc gtgaacaagc cagccttcaa gatcgcatta accttaaaac
    23641 aaatacatta ggtgctgccc tagaatcttt aggaggagcg tgggaaacct ttgtggggga
    23701 tattggctct gtctttgccg aagatataaa agggttttca aaaggcttgc aatctgcggt
    23761 ggaatggctt gtgccatttg tatcaaaaca taaagaggcg attaaatttg taggtggttt
    23821 ggttgctgga cttgttattt tacgaggcgt tttacaagct ggttcttttg ttattcaaac
    23881 ggtaactatg gcaatgaacg ccctcaaaat tgcagcaatg accaatccta ttgtcttagt
    23941 cattggagca atcgcagcgg gtgcttactt gatttatagc cattgggata gcattgcccc
    24001 ttggtttgca aatctttggc aaaatgtgac cgcttacttc aatcaatttt ggacttgggt
    24061 aaaaggtgtt tggtcgggcg tgacggactg ggtgtcttct gtgtggaatg gtgtagccac
    24121 ttttttcggg cagttatgga ctacgattac tagcttattt agtgtagctt ggacaaatat
    24181 ccaaacaata tggggaggag caacagcttg gttctctggt ttatgggatg ggattaagtc
    24241 tgcggctttt ggtatgttcg acagtattag ccaaaaagtc agctctgttg tagcaagcat
    24301 taagagcttt ttaggctttg gcgatgatgt ggataaaatg ggggcaaaaa ttggcaatgt
    24361 agctacccaa gtcatcagca aagaacactc aggacaactc agcaaaaccg cagatatggc
    24421 agccaataca gggttctcta gcggtggttt caccgggcaa ggcggtaaat atacccctgc
    24481 cggtattgtg cataagggcg aatatgtgat gacgaaagaa gcgacctctc gtttgggtgt
    24541 tggaatgctg gatagcttaa actatggcgg tgctatgggt ttcactcaac ctgttttgca
    24601 accgatttta ggtaagcaaa aaggcttttt tggctcactt tgggacgatg tgaaattcgg
    24661 ggcaaacttt gtcggcaatc tgcttggctt aaatcaaggc agcctgaaaa cgcctgattt
    24721 taacccaaat gcgaccaatg caagcggtca aaattcgtcg atttttagca attatgaacc
    24781 actcaaccga aatgcggtaa cacagcagga aagctcgcaa gcaccgcaga tagtcgtgaa
    24841 ctttaacccg acaatcaatg taaacggtaa tgctacgcaa gaggctcaat cgcaaattat
    24901 gcagcagttg caaatgagtg cttatgagtt tgagaaattg ctcaatcgtg tgctagacca
    24961 acgaagccgt agagcctatt aggagtgatt tatgtatttt atgttaggca atattgcctt
    25021 tgagccggtg aatttaactg aatttagcga gcaacatacc gccgaatttg ccgaacacgc
    25081 tgtgctaaaa ggcaaacctc gtttacaagc gatgggcgaa aagctaaatg aacttaattt
    25141 ttctcttcgg ttgcaccata ccataggcgg tgtagagagc cgttatcaag ccttgcttga
    25201 agccaaagcg aagcaagcgg cattggcgtt aatttggggc agtcgttata agggagatta
    25261 tgtaattgtc gatatttcat cgaccacatt gttcaccgat ggcagaggca atgccctcgc
    25321 tcgtgaaatg caaatcagcc tgaaagagtt tgtgggcgat aatgacggtg gtgtgttggg
    25381 tgaggcgtta aattttggtg gtggctcact gcttggctct attttgccaa gcggtgcggt
    25441 aactacccta tctcaaatca aaacagcagt cagccgaggt gtggagcttt ataacagcgg
    25501 caaacgctta gttgacgaag tgcaaaatac cgtggcgatt attcggcagt tgaaaaatga
    25561 cccggcaact gcattggcat atttacccgc tgcattagcg aatttagacg gggcattggg
    25621 gcattttggt aatttgaccg gctcatcggc gaccttgggc ggtttgcagg agaaactacc
    25681 ggcagcagtg gtctttagcg aggaaatcgg cgagatttat cagagtttgc aaacaatgcg
    25741 aagcagtttg ggtaatgcct cggcaaatag ctgggataat tggtttacac cggcagagag
    25801 tgcattgaca gaggtcacag acagctttga ccatctagca aaaccggtgg caaaaatgac
    25861 cgcttggatt gtgctgcgta ccgatgagga ggcagaaaat gacacagttg cttaaacata
    25921 tcgtcaaaca gggtgagcgt tgggataact tgagctatca atactacggc aatgcgtttg
    25981 aatatggtcg aattattgat gccaatccgc atatcggttt ttgcgaggtg ttgccgatag
    26041 gggtaacaat ttatatccct gtacttaacg ttaagccaac gaataatgaa aatatgccac
    26101 cgtggctaag ggggcgtgat gcgtaatgta ccgaagcccg atttttcact gttttatgaa
    26161 aaaaccaata tcactgccga gattgagcca catttggtgc aactgaccta tatcgaccat
    26221 ttggaaggtc aatcggacga actcactgtg gaatttgaag acattcaagg caaatggata
    26281 cgccaatggt tcccgactca gggggataag ctcaaagcgg cgattggcta tcagggcgaa
    26341 tttctgacgg acatcggtga gtttgaaatt gatgaagtgg aatatagtta taagccttcc
    26401 actattaact taaaagctct aagcaccggt atcagcaaag ccaaccgtac tttaaaaccg
    26461 aaagcctatg agaatacgac tcttgctcag gtggtggcaa aggtagcaga taacctgaaa
    26521 ttaaaggtag tcggtaagat taaggctatc ccgattaaac ggatcaccca atatcaagag
    26581 cgggacgtgg agtttttggc acgccttgcc cgagagtatc atcacagctt taaaatcgtg
    26641 ggcaatcagc tggtctttac cgataaaagc gaactggggc aaagtgagcc ggtgttaatg
    26701 ttggaagaac gggatactat ttccctacgt ttgcgagatc ggattaaaga cactgcaaag
    26761 gcagtggaaa tcagcggcta tgatacaagc ggtaaaaaag tggtgaaaaa acgcaaaaag
    26821 gcaaaagcct tacgcccgaa tataaaacaa gctaaatcag ccagtgaaga tacgctgaaa
    26881 atcatcacac gaggcgaaag tcaagaacag attgatgcac ggggtgaggc tgcgttggct
    26941 acgcaaaatg aagatcaggc ggctggtgat attgagctta tcggcaatcc taaattggtt
    27001 gccggagcaa cgattttatt gcgtaacttt ggcgtgttta gtggcaagta tctgattaaa
    27061 tcatcacgcc atacgattag ccgtagcggt ggttatacta ctcatattga ggtcagaatg
    27121 ttggagttta ttgccgatga tttaatcaca ttaggtatgg aggctggcaa tgcaaacgca
    27181 taattttggg gcgacttatc aagaagggat tgtatccgct gttgatccga aaacgcacaa
    27241 agtgcggtgt aaaatccctg cgctggaaga tttagaaact gcttggctgt cgtttttgac
    27301 cccgaacgcc ggtggcaatc agttttactg tttgcccgat gtgggcgagc tggtggcaat
    27361 gctacttgat gcacaaggcg aaggtggctg tgtgtttggg gcaatttata acgagcaaga
    27421 cccgacaccg gttgccgata gcgatatgtg ggttaagaaa ttcagcaatg gcacggagat
    27481 ttcgcataat cgcaaaaccg gcgatgtggt agtgaagact aacgggcatt taaccgcaac
    27541 ggctcaaggt ggggctacga ttaatgcaaa caccgtagtc aatggcacat tgcacgctac
    27601 cggtaaaatc acctctgacg aagaagtctc tgctcctgtt gtgaaacaag gcacggtaga
    27661 gcttggcaca cataaacacg gcggtagccc tgaacctaat aagtaattct ttaaaccagt
    27721 ttaaaatccc ttttaaagat agccctgtat catcagggct atgaacagac aaacttccat
    27781 tcaattaacc cactggcaac ttgcaccctc aaccgataca gttgaggtgg tgcaaggtat
    27841 tgatgatatt catctttgta ttgtcaatat cctttcaacc caaaaaggca cggatatttt
    27901 acgccctgaa tttggctctg accactttcg ctacattgac taccctgaag atgtggctat
    27961 cccgaatttt gtacgggaaa ttacaaccgc cttgcaaaaa tgggaaaaac gcattgaggt
    28021 tgaagaagta ttggtggacg gttcagctcc gcattttatt tttactgtga tgtggcactt
    28081 aaccgatgat gtttatcgtg aaatttaccg aacaacggta taaggatagg caatgacaga
    28141 agcgattaaa atcattgatg acgatgtaaa aaccgtactt gccgaagcga ttgccgatta
    28201 tgaaaaacgc accggtaaaa tcttacaacc tgcacatatt gagcgttcca ttattcaaac
    28261 ctacgcttac cgtgagcagt tattacgcaa aggcattaat cacgcctttt tacaaacctt
    28321 tccgcagttt gcgacgggga ttgcgttgga tttgtgcggg gaaacttttg gttgttatcg
    28381 gcttagagat aaacctgccc gtactttact acgcttttca gtaagtggtg aacatacagc
    28441 tatagttatc cctaaaggaa cgcaggtggc aattaatgac gagctatact tttcaacgct
    28501 caatgacgat gttattactc cattaattac ctatgtggaa attgatgcag agtgtaatca
    28561 ggttggtgtt atcggtaatg gttgggaatc aggaagagtc agtaaactaa aaacagcact
    28621 ggcaactaat tataaagtaa cagttgaaaa cattgatatt acgagcggtg ggttgatgca
    28681 ggaagaagat gaaccttacc gtaagcgaat tttagccgca ccggaagcct ttagtaactg
    28741 tggctctatc gctgcatatg attaccacgt ccgctctgta tcccaagata ttgcagatgt
    28801 gaatatctct acgcctaggg gtggcttggt aagaattgcg gtattgaccc aaaaagggtt
    28861 accggatacc cgattacttg atgatattaa aaaacacgtt agtgccgaac ggctacgccc
    28921 actttgcgat atggtcgagg ttattaagcc cacagaacgc cgttatcaaa ttacggcacg
    28981 tttaatattg ttagatggct accgtgagga tttggtaaaa gcaaaagccc gagaggcatt
    29041 acaactctat ttgtcagaca agactaaaaa actcggttta gacattgtgc cgtcggccat
    29101 catttcagct ttaagagttg agggtgttta cgatgtgaat ttaattcagc cagtcaaaac
    29161 tattatcaac gaaacagaat gggcgaactg cacagcaatc agcgtagaag tcgaagagga
    29221 gcgtagagat ggctagcttg cactatgcgg acattattgt gcgagaccct aaatataaag
    29281 cattagcgga tttaagcaag cggttaaatt tgctcgatac ttcgcaaatt atgactacgc
    29341 tggtggattt attgggcgac gaatgtatcc cattgctggc agaaaaatgg tcggtaaccg
    29401 gctatgacgg cttattggtt gcagatagcg aaaactcaaa gcgagcctta attaaaagag
    29461 caatcgaact acatcaacta aaaggcacgc cttggtcaat tcgtgaagta ttacgcagac
    29521 taggtttcgg agaggttgag attgacgagg gactgaaaaa tcggctgtat gaaagcactt
    29581 atgtgagcgg aatccccgaa aacgaacgct gggcgcacta tgcgattagg ttgagtaacc
    29641 cgattaccaa tgagcaagct cgcaatatcc gtaaggtctt acgcaatttt gctccggctc
    29701 gttgctctct tgcagttttg gattataaag cagatccaat tcgatataac aataaagcaa
    29761 cctacaacgg cacatataac cacggttcaa gttaaggata aattatggca ggtgtacaag
    29821 aaaccgcaca gtgggaaaac cacgtttaca gaattgaaga aaatgaccca gtccacggag
    29881 gagaaaacgg tgtaaccaat aagccaatta agcacctagc aaaccgcacg ctctatctgc
    29941 gtagattgct caccgaggca ggacaacgca ttaacccgaa aaaaatcaca gctaccagca
    30001 aaaatagtaa tgatttaact gggcatactc acgagattga ccacgcaagc ctaacgcaaa
    30061 aaggcattgt gcagttaaat gcgagtatcc aatcaactag cacaactgag tctgcaaccc
    30121 cttcagcagt taaagcagcc tatgataaag cagcaacagc agaacaaaaa gcgacattgg
    30181 caaatgagaa agtcgaggcg attaaagtag gcggacgaaa cctcctcatt caatccaaca
    30241 ctccacacaa cgggaatgcg tactcagtca gatacgcatt ggctgaggct ccgaaagtag
    30301 gcgaagagat tgtagtcact ctatggggtg aactcggctc tcaacgtact ggaattggcg
    30361 tttacaactc tcaaggtgct acggagttgg caaaactggt caaaattcgt gatggagttt
    30421 atcaaggtaa aggcatttgg cgtaagccga tgagtgaggg acgagagcga acgcctaacg
    30481 acacgcacct gaatgtgtat ttttacccga aaaccgcaac agataataac cgtattgagc
    30541 gaatccagct tgagcgaggc tcaatcggta cagattggac gccaaacgag gcagacttaa
    30601 aggcagagat tagtgcaatc cgcactaaac taatccctgt acggggtggc aatgttgcta
    30661 caaccttaaa tgcggctaag cggtcggatt ggtttagaga aatgcaaatc ccttatcaaa
    30721 gtggttgggg gtattacacc caagcatttc ataactcagg gacgcaacac aactacagca
    30781 atttaccgct gacaggtcgt tattatttta tcgccgaact ctatcataac tctggttatt
    30841 ctcacttgcg tatcacctat ccaagtttaa aacgcacctt tgagagtgct atcaacctta
    30901 acaatgagga tttgattcta gattggcgtg aggtggtgtt tttaaaagat ggattgtatc
    30961 aaggcacttt taaaacaaca ggcacactag agtctgctta tcgtctaaga gcggctcgtg
    31021 atgatgaacg ttatcagcca tacctgcaaa tggtcgatat gggctatgac cttgccaatc
    31081 ccccggtagg aaaaaacaaa attattggca gtgttgatta ttacgttaaa aacggcacaa
    31141 gcgagtcttc caaagcgacg atgatggcag cggcaatgcc tgacaagaac gtattttggg
    31201 aagtagcatt gtggaatgca gcacagcaac ggaatgtatt gtttaagggg tatagcaaga
    31261 ctaacaattt gagtattggt aaaaccaccg acaatgagcg agaccgagta caagtcaatg
    31321 gcacaatcca agccaccgca ccggctgata acgccaataa cgaccaagtg ccgactacgg
    31381 cttgggtgcg taagatggca aagattttag ctgatggcaa ggtgagtaaa gccggtgaca
    31441 caatgactgg gcatttattc atcaagaatg gggaatactc tgcggtacat acctataaca
    31501 caagtggttg gttcgcaaaa tgggaggcgg ccccattatc tgattcgcac tttgctgcca
    31561 ttgtatatgc caacgacaga gagacagtta tcaatcgggt gttaattccc aagaagaatg
    31621 gcacagtcgc tttaattaat gatattgccg ataccgttag agccgctgtg agtcgtagtg
    31681 ataaatcccg tagtagaact tttcctctgc aaacggggaa aaaatataca accacaggga
    31741 gcgttacgat ttatccagat ggccgaattg tgcagatttt ccacttaaaa aatattaaag
    31801 cggcgtggtt tgattatgag gcaagtgcgg taggagatag gcatagagtt gtcaatatcc
    31861 ctctttggac tgcgatgcca aataaaattt ttgatgtcga tgttaaaact gttcgcccct
    31921 caaataatgc ctcaacttac tacatagaag cggctgaatg gctgagtgcg tggcagattt
    31981 acggtaatga cacgaacaaa tcaagtgtca atattaatct atctcgattc agaggtggaa
    32041 aggatgaaga tatggacttg tatgtaacag ttgagggata ttaaatggca tattttgtga
    32101 atgtgattga tgagcaaggc aattatgagc taattgatga tgagtttatt gggctgtatc
    32161 cggatttgga ttttaacaca ttacccaaac tcacggataa gcaatatcag acctaccttg
    32221 ccaaagcaaa cggcaaagag cgatttgtaa atggcgaatt tgtgtatgag caaattaagg
    32281 tggatatgca agcggtcatt tccgctgaaa aatctgcaaa gttagcagag attaaccaaa
    32341 aagcacaagc ctttatcaat gatttagcca aatacaacga aacgcctaag tttgagcgtg
    32401 atacttggct tgagcaagcg aaagaggcaa aagcgtgggt ggctgaccca accgtacaaa
    32461 cgccaaccct tgagttaatt gcgcaaatgc gaggcatacc gctcgacacg ctacgccaaa
    32521 aagcctacga aaaagcgatg gcgtatcaaa cggtggctgc tattgtagca gggcaacggc
    32581 aaggatacga agaccgccta gaacaagcgg aaaccttaga gcaaatacag gcgattgagc
    32641 cgatgtatca attaccgcaa ggagcaaaca atgagcaaac tgactaaatt aaaatcgtgg
    32701 agctaccacg tcttaattgc aatagatcaa ctctttaacg ctctcaccgg tggagcagcg
    32761 gacgaaacat tttctagccg ttgctaccgt ggtgcagtgc ttgcggaaaa tccgaaaaaa
    32821 cgttggcgtt tttggcactc ttttttgaat tgcttatttt tagacccaaa gcactgctac
    32881 gccgcttata aagcggaggt acaccgcaag cagtatccga aggcatttag acttgggggg
    32941 atttaatgaa aaagcaaaac ttaaaattag tgcggggcga tgatgtgagt tttagattca
    33001 gcattaaaga cagcaccgga caacctcttg cgttaaagga tttgtggttt gatttgcact
    33061 tagtcagcta tcaaggtgag gtgctgattg ctctctctac tgatgatggc agtattgaga
    33121 cagcagaggc tggtcagatg gtgttacatt ttagtcacca gcaaactgct cgagctgaat
    33181 ggacaagtgc caattatgac ttgcaactca cgcacgacta tacaagagtc taggaggtgg
    33241 agatgacctc tatcattgaa ctagacctga ctgatgacct catcattgag gtgcagttag
    33301 atagcacaat cattaccggc gatagctttt tagccaacct gccggattta ctccaaattt
    33361 acaaccaagc aaagtcagat agcagtaagg agcttaacaa tgactaaaca caaaaaccac
    33421 gaaaaccacc cgattgctat aacattctac caacttggca aagatgtcgg cgacattaac
    33481 cgccggctag cggttattga gcaacgtgag caatcagcac agccaagcca accacaagct
    33541 gaggcggtta caatgccaca gcaagatgct gttgccatta ataacatcaa tgccgttttg
    33601 gaggcggtcg aaaaatacaa aacttttgaa aaatacaaca ctcagtggca agagcgtttt
    33661 gaaaaattcc tgtctcgtaa cccgaatgat tacgagttaa gtgaagagca acgccaaaaa
    33721 ctttaccaca cgatgtgcca gcatttgtgg caggtgaact ttactgaaat caatagtaaa
    33781 cagtatgaat taccgatccc gcaacaattc cagggtaagt ttggctatat tgtcaaaacc
    33841 tcgggcggta gcacagaaat tagttttgag ttgctatctg aaactcacag cacttatctt
    33901 tatccggata gctatattcc cgagggcggt ggcgatgtaa tgctcaagcc ggcttatatt
    33961 tatttagtga ttccggactt agtggcaatg cgtgagccgg aggtggtggg ggtttcaccg
    34021 ctgaaactga acattctgca gaaacacctg ctgacacctc agaaactaca ggagcagaag
    34081 cccttgaaca cgaaggcaaa ctgattgatg gcgtatggca ttttgagcga gagccggtaa
    34141 atccaccgag agatcagaat tattatgtgg caggtaacct gttctacaac ggcataccgg
    34201 attgataaat tgatgtttca gtaaacctaa ttagggggga atcccccttt tttattatta
    34261 taaggaaaga aaatgaataa atctttagta gcgttagctg ttgcagcgat ggcatcgaaa
    34321 gcacaaacta aacagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc
    34381 aagcaagcaa gcaagcaagc aagcaagcaa gcattgtgag atagagaaag tatttaaaca
    34441 agctccttta cccttttcag ggcaaaaacg gatgttttta tcccatttca aacaggtgtt
    34501 aaatgcaaat attgaagcag acgggaaaga ttggactatt atagatgttt ttggtgggag
    34561 tgggttgctt gcacataccg caaagcgtga gaaaccgtta gcaagagtga tttataatga
    34621 ttttgataat tacgccgaac ggctaaacca tatcaaagag acaaatcaac ttcgccaaga
    34681 aatctaccaa attgttgatg aaattatacc aaaaaataag agaattagca atgaaattaa
    34741 agctaaaatc atcaataaaa tcaatgattt tgaggggttt aaagacctca aatgcttaag
    34801 ttcttggtta ttgtttagcg gtgagcaagt tgcaacttta gatgagttat ttaagcacga
    34861 tttttggcac tgtgtgcggc aaagtgacta tcctgaagca acgggttatc ttgatgatat
    34921 tgaggtggtt agtgagtcct ttcatcaatt attgccgaga tttcacgaca aagagaaagt
    34981 attattgatt cttgacccac cttatctttg cacaagacag gaaagctata agcaggaaaa
    35041 ttactttgat ttaattgatt tcttacggtt gatagacctt actaaaccac cttatatctt
    35101 ctttagctca accaaaagcg agtttattcg ctttattcac tatatggttc aaaacagaaa
    35161 agaaaactgg caagcgtttg agggagcgga aagaatagtg gttaatgcct cggcaagtta
    35221 taacggcaaa tatgaggata atatggtgta taagttctaa aaatttaaac gccctttgat
    35281 agtgatttaa agggcgttaa aaaaccatta aaattaagaa agtaaagctc ctgttttctt
    35341 tcgtagtttt cgtggttgcg tttcgcagtt ttgttggctc gctacaatta ctgttcattg
    35401 taatgtttaa tcgaccattg gttttgctag gatcagtttc accaaaataa ccgttaaaac
    35461 cttggacggt gccttctgct gctttgattt tttcagcaag tatttgttgc tttgcagcac
    35521 tttcatctgt taccttgagt acttcaaagt attcgttttc acgaatgaga ccagtagcaa
    35581 agtctaaatc gatgaaaact ttacgcgcat taaaaatagc tgtgatctct gcaacgctta
    35641 aaggtttttt caaacggata aaattagttg gcaagtgtgg aaaacgttga tatgtgaaaa
    35701 tatctttgtc caaatgacga gaaacctgat ctggactagt aattgcttga actttcactt
    35761 ggtcaattgt tggactaact atgccggtta gtgtaatgtt tgctgtttgg gctgtattat
    35821 gattgacaac catcgctcct tcatcgctat tctgaatacg gcggaacgtg agatcattgc
    35881 cgttgacatc taagcgccca ccacgataac caaaatagat attgttagga tccatttgat
    35941 ctgatgaatt gaggattacg gtaggtctgc cactcacaat acctagctct gaaaatgcag
    36001 attttttgtt attttcatcc gcttgttgat ttaatataac agtgccgtca ccaacgctaa
    36061 cggagccttt gttttctcct ttgccgttga tagttagtgt cccttgtccg atttttgata
    36121 atctgtcacc agcagggtta ctgattttcc agatcacatt tttatcttta tcaatatcaa
    36181 caccggctcc taaccaagta atgcctgctt tcataccttc cacagtggta ttaccatgaa
    36241 aatggattgc tcctgcacct tggttgatat tctctgtgag acttagcgta ttgttttcac
    36301 ccaaaatatg gaatgtttta ccgtgttgga gttgaggatt ccaagggtta tcgcctgtat
    36361 taccattatg accttgaagt gttggtaatg aagtatctgc taaattaact gtaagaggag
    36421 cattccctcc tacgattgag ctggtattgc cagaaggtgt ccaagtgagt ctagcaccat
    36481 ttgcattaat agcgcctgca ttattttctt tttctaaagc acgtacataa tgtgggtggt
    36541 gatagttcca gtaagattta taaacatctc ccccaagcgt attattctct gagaaatagt
    36601 cactatatac acctaacaca acccattttt tggtacgaac atcataacca aaaagagcgg
    36661 agccactatc gccaggtaaa ccgagagttg ttaagggacc gtagccgtgg tctttaaata
    36721 catcgtttat ggtattattt tcagtttttg ctactaaaat acggctgtta aaatcgctgc
    36781 ctggaacgct tggatcgcca ttagtaacag gcagaacgct acctcctgtc aaaaaaggac
    36841 cgttaccgac ttcaatacgc ccgttgttta agcgatctct ttcgttgtta accactgctt
    36901 gtctaccaga accggcacga ataaacatgg ggaaaataaa agggtcgcta aatacactat
    36961 aaaaatcttg tacattttcg gtagtgcgtt caatctcatt agctggagcg acttcagtta
    37021 ctaatttcga taacctaggt gcgtggtagt cccagcgggg cgttgggtta cctgaaccat
    37081 ctaaaatcag tttttctcct tcgtcattac gttcacgttt ttctatgcct ttcccgagat
    37141 ctgttctttc tttatttgga tcaggcatat agttattacg gcgcaccaca ctataattgt
    37201 aatgatggtc gtcggctttt tcgctagatg aatcaccaaa ttgaatgtcc cgatagcctt
    37261 ggttgtggtt tacactaata acgtattgtg gattaactaa tgttgcaatc cctgtttgac
    37321 gagtaatgga agagaagtca atcattggaa tagacatagt ctcacctgtt actgtttcaa
    37381 aaacaggtag atcattagag tcttcatcac ctccaccata gagaaattca taacaaccgg
    37441 gtaaactttc attatcaaag cattcagata ggcttacatt cggcttaggt gaaatatttg
    37501 attccacttc aacctgctta gttggtgtaa cctgtaaaaa agtaccgatg tttttgcccg
    37561 ttttatcaat aactggaata ttggttgccc caacaaaaaa tttgccttta ttttctgcta
    37621 aatcacgaaa atattgataa tcgacatcac cacgtacaat agatgccagt aatgttggtg
    37681 aaagagaaat agcaattgat gttgctagaa gagagagctt gaaagtatgc ttcattatta
    37741 tttgaaacct gtataaatat gtaagtagat cagatttgag cgctattaaa aagattattt
    37801 aaatcgatta aatattcatt taaataccac acaattattc agcgcgatta tcttgttcgg
    37861 cataaatctg ctc
//
LOCUS       DANLXQ010000005        36124 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.005, whole genome shotgun sequence.
ACCESSION   DANLXQ010000005 DANLXQ010000000
VERSION     DANLXQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 36124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36124
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.005"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            5..988
                     /locus_tag="PXD94_000204"
     CDS             5..988
                     /locus_tag="PXD94_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL6285113.1"
                     /translation="MLFKIKKYLLLFWVISACYTFAWAESTIKTPLAYLTAMSEAHKK
                     STYELVYILQQGSDTESFRLRHAFTHNKEYAQLLNLDHSREEIILKNNKVSYLGYNFR
                     PFSLNSSQILDNLPNVLYTDYANLKGYAFLDAGKDRISDRITKVIRMKPNDTLRYAYI
                     LWIDEETHLLLKSQVLNMDNLVLEEFRVLQLYQSPELDRIANVIESLMLPTLTTVKKE
                     EFPKSDGWQVKWLPTGFRLIQQQTVTGATYQVESEYVDSRVYSDGLSTLTIYIMPSQG
                     VSFNEYAWQQGKLTILNQTINDRDIVIIGDVPLQSAKQIMNNIGFKEGAQP"
     gene            985..1449
                     /locus_tag="PXD94_000205"
     CDS             985..1449
                     /locus_tag="PXD94_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL6285114.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCVAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            1427..3112
                     /gene="fadD"
                     /locus_tag="PXD94_000206"
     CDS             1427..3112
                     /gene="fadD"
                     /locus_tag="PXD94_000206"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL6285115.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFAATLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            3137..3766
                     /gene="upp"
                     /locus_tag="PXD94_000207"
     CDS             3137..3766
                     /gene="upp"
                     /locus_tag="PXD94_000207"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL6285116.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(3877..4743)
                     /gene="rsmA"
                     /locus_tag="PXD94_000208"
     CDS             complement(3877..4743)
                     /gene="rsmA"
                     /locus_tag="PXD94_000208"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL6285117.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNFRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLDALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(4805..5755)
                     /locus_tag="PXD94_000209"
     CDS             complement(4805..5755)
                     /locus_tag="PXD94_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL6285118.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTTDINSGKISFEEAAKTHSV
                     DYASGADGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQYVGQ"
     gene            complement(5828..6382)
                     /gene="pyrR"
                     /locus_tag="PXD94_000210"
     CDS             complement(5828..6382)
                     /gene="pyrR"
                     /locus_tag="PXD94_000210"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL6285119.1"
                     /translation="MAREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            complement(6396..7823)
                     /gene="hldE"
                     /locus_tag="PXD94_000211"
     CDS             complement(6396..7823)
                     /gene="hldE"
                     /locus_tag="PXD94_000211"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL6285120.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLGVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVVINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGVVSEAELKAIVE
                     EAKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            complement(7919..9454)
                     /gene="der"
                     /locus_tag="PXD94_000212"
     CDS             complement(7919..9454)
                     /gene="der"
                     /locus_tag="PXD94_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL6285121.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIEAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEHQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILFQEGNNPFAGKKNKLTPSQLRKRKRLMKFIKK
                     SKK"
     gene            complement(9639..10946)
                     /locus_tag="PXD94_000213"
     CDS             complement(9639..10946)
                     /locus_tag="PXD94_000213"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL6285122.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMSTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            11200..13398
                     /gene="relA"
                     /locus_tag="PXD94_000214"
     CDS             11200..13398
                     /gene="relA"
                     /locus_tag="PXD94_000214"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL6285123.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSIHTVVLGEGEKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            13517..14713
                     /gene="ispC"
                     /locus_tag="PXD94_000215"
     CDS             13517..14713
                     /gene="ispC"
                     /locus_tag="PXD94_000215"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL6285124.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNPNQYEVFALVGGKNVELM
                     LEQCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAA
                     IVGAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQ
                     SLPPEAQQKVGFCPLAELGVSKIILTGSGGPFRTKPLNEFDAITPAQAVAHPNWSMGK
                     KISVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNP
                     DMCTPIAHTMAYPNRINAGVPPLDFFKLKELTFIEPDFARYPNLKLAIEAFAEGQYAT
                     TAMNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAK
                     QAVLQL"
     gene            complement(14756..16138)
                     /gene="nhaC"
                     /locus_tag="PXD94_000216"
     CDS             complement(14756..16138)
                     /gene="nhaC"
                     /locus_tag="PXD94_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL6285125.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSSIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(16184..17770)
                     /locus_tag="PXD94_000217"
     CDS             complement(16184..17770)
                     /locus_tag="PXD94_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL6285126.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIAFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSLGAHYLDEQN"
     gene            complement(17833..18639)
                     /locus_tag="PXD94_000218"
     CDS             complement(17833..18639)
                     /locus_tag="PXD94_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6285127.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            complement(18654..20624)
                     /locus_tag="PXD94_000219"
     CDS             complement(18654..20624)
                     /locus_tag="PXD94_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL6285128.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEDPQSKEA"
     gene            complement(20626..21579)
                     /locus_tag="PXD94_000220"
     CDS             complement(20626..21579)
                     /locus_tag="PXD94_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6285129.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            complement(21791..23374)
                     /locus_tag="PXD94_000221"
     CDS             complement(21791..23374)
                     /locus_tag="PXD94_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6285130.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            23596..24753
                     /gene="hemW"
                     /locus_tag="PXD94_000222"
     CDS             23596..24753
                     /gene="hemW"
                     /locus_tag="PXD94_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL6285131.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLIEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALADLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKIKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREVVRPTIDWALNQNYISETATHWQITQHGKLFLNELLEGFLE"
     gene            24896..25378
                     /locus_tag="PXD94_000223"
     CDS             24896..25378
                     /locus_tag="PXD94_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL6285132.1"
                     /translation="MIDKQIRNSTAEFLIFTQQNGAENITVRYEDNEIWLTQKLIAQL
                     FDCSTDNVSLHLKNIFNSRELDPNSVTEDFSVTAADVKNYRTKHYNFDAVISIGYRVN
                     SVRATQFRQWATKVLRNFAQKGYVIDRQRMENGTFLNEDYFEQLLAEIREIWLSERRS
                     "
     gene            25347..25544
                     /locus_tag="PXD94_000224"
     CDS             25347..25544
                     /locus_tag="PXD94_000224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285133.1"
                     /translation="MKFGYLNGVLEGKGKMRFDEAKLHAESEFEKYRIVQDRLFESDF
                     DKVLKEMLRSENQYRPSFSTT"
     gene            25631..26098
                     /gene="bcp"
                     /locus_tag="PXD94_000225"
     CDS             25631..26098
                     /gene="bcp"
                     /locus_tag="PXD94_000225"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL6285134.1"
                     /translation="MNILQAGDKAPKFTLQNQADEPVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPDHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGGGEQVFDKFKTGEHHQMIVDYLRGR"
     gene            26098..26646
                     /gene="gmhB"
                     /locus_tag="PXD94_000226"
     CDS             26098..26646
                     /gene="gmhB"
                     /locus_tag="PXD94_000226"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL6285135.1"
                     /translation="MGKKAIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     KDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTDKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            26651..27490
                     /gene="queF"
                     /locus_tag="PXD94_000227"
     CDS             26651..27490
                     /gene="queF"
                     /locus_tag="PXD94_000227"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL6285136.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDSLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVGKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STHYLENIAEDELVEETLVSHLLKSNCLITSQPDWGSVQIRYKGKKLNREKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAEPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            27682..28815
                     /locus_tag="PXD94_000228"
     CDS             27682..28815
                     /locus_tag="PXD94_000228"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL6285137.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVCGGGSRNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(28812..29870)
                     /locus_tag="PXD94_000229"
     CDS             complement(28812..29870)
                     /locus_tag="PXD94_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6285138.1"
                     /translation="MAEILFRSCLCCNAKRIFYLLTFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NARAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALKGILNEQFIFVHLLLFIPLGLGSA
                     FYIIFPYIFTNLLDKPELVSVSFFVNNLIAVLLQSDFSRKLNLTVHKLIYIAPILIIL
                     QIMPWFYALQWLSTITAFLYLVIFATITVYANTALSNMLVKLDNGKNQGLMFGSSKLI
                     LAITTLGVMNVLPYIFLI"
     gene            29953..30414
                     /locus_tag="PXD94_000230"
     CDS             29953..30414
                     /locus_tag="PXD94_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL6285139.1"
                     /translation="MRKIKLLHVILSLILGLSAAAIGSYPAIKEIGEERAKKHQDILD
                     LNRELYSAMVNRDFSALQTLLSDDFELYSSTGEVIDKQEWIKLIQKRVLEFDKVDLLS
                     TDFQGNQSSGLLRVNGEFFGIERDNVVVEMNIRTIVRNTERQIKCIVIKEV"
     gene            30427..31320
                     /gene="murQ"
                     /locus_tag="PXD94_000231"
     CDS             30427..31320
                     /gene="murQ"
                     /locus_tag="PXD94_000231"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL6285140.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(31404..32348)
                     /gene="prmB"
                     /locus_tag="PXD94_000232"
     CDS             complement(31404..32348)
                     /gene="prmB"
                     /locus_tag="PXD94_000232"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL6285141.1"
                     /translation="MFKYNTELLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            32441..32986
                     /gene="smrB"
                     /locus_tag="PXD94_000233"
     CDS             32441..32986
                     /gene="smrB"
                     /locus_tag="PXD94_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL6285142.1"
                     /translation="MNDTLLDDEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            33050..33535
                     /locus_tag="PXD94_000234"
     CDS             33050..33535
                     /locus_tag="PXD94_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL6285143.1"
                     /translation="MSEQTFHQLTALLDSHRARYRVVGHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(33537..34286)
                     /locus_tag="PXD94_000235"
     CDS             complement(33537..34286)
                     /locus_tag="PXD94_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL6285144.1"
                     /translation="MEKRFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            34458..35321
                     /locus_tag="PXD94_000236"
     CDS             34458..35321
                     /locus_tag="PXD94_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL6285145.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
     gene            35445..36038
                     /locus_tag="PXD94_000237"
     CDS             35445..36038
                     /locus_tag="PXD94_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285146.1"
                     /translation="MKLKALSTLIASSMLYSQSFAVPPEKTAEEPQPFFNKTESGYDL
                     VFNDQNYRLIETELNGEKIQFRAFERIVYVNNPIEPDYQTINLYVPEAYYNNGEINGY
                     QADTAPIFLPNSVGGYMPAKAASYDKKRRDGSPGTILVALSKGYVVASVGARGRTLQK
                     EGKYTGKAPSAIIDSKSAAHKACRFESDHWHHSLCKI"
BASE COUNT        10782 a         7124 c         7826 g        10392 t
ORIGIN      
        1 aaatatgctt tttaagataa aaaaatactt attgttgttt tgggttattt ctgcctgcta
       61 tacatttgct tgggcagaat caactattaa aacaccatta gcatatttaa cggctatgtc
      121 agaagcacat aaaaaatcca cttatgaatt agtttatatt cttcaacagg gaagtgatac
      181 agaatctttc cgtttacgtc atgcttttac ccataataaa gaatatgccc aattactgaa
      241 tttagatcac agccgagaag aaattatttt aaaaaataac aaggtcagct atttggggta
      301 taattttcgt ccttttagtt taaatagttc acagatttta gataacctcc ctaatgtact
      361 ttacacagat tatgcaaatt taaaaggtta tgccttttta gatgctggta aagatcgtat
      421 ttctgaccga attacaaagg taattcgtat gaagccaaat gatactttaa gatatgccta
      481 tatactatgg attgatgaag aaacacattt attacttaaa agccaagtct tgaatatgga
      541 taatttggta ttagaagaat tcagagtttt gcaactttat caatctccag aattggatag
      601 gattgcaaat gtaatagaat cactaatgtt accaacatta acgacagtga aaaaagaaga
      661 atttcctaag tctgatggtt ggcaggtgaa gtggttgccg acagggttta ggttaattca
      721 gcaacaaaca gtcacaggtg cgacatatca agtggaaagt gagtatgtag atagcagagt
      781 atatagtgat ggtttatcta cattaactat ttatattatg cctagccaag gcgtgagttt
      841 taatgagtac gcttggcaac aaggtaaatt aacgatttta aatcaaacga ttaatgatag
      901 agatattgtg attattggtg atgtgccgtt gcaatcggct aagcaaatta tgaacaatat
      961 tggttttaag gaaggagctc agccatgatg atcgaacagg ctacggtggt ggaatatcag
     1021 aatggtgtgg ctattgtaca atgttatgct aaaagtagct gtggtggctg tgtagctcag
     1081 tctgcttgtg gcagcaaagc attatcagca ttagcaggtg aaaaaattga ccctcgtttt
     1141 cgtattgagg tagatgaacc cctaaaagta ggcgataagg ttgaactagg gttagctgaa
     1201 aatatcttgc tcaaaagcgt gtttttaatt tatggcattc ctctgctggt tcttgtgatg
     1261 acagcggtcg gattttcgca attttttgca aatgagctta ttgttttatt tataatgttg
     1321 ctttcaacag gtataacatt ctgggctgta agaaaattga ttcataaaca cagcaaacaa
     1381 gcaaattttt cacctatctt tttaggtaag gttttaggag actataatgg aaaaaatatg
     1441 gctaaataat tatccgcctt attcaccaaa aacgattgaa acagatcaat atgaatcttt
     1501 agtggaaatg tttgaagcag cgatacaacg ccaccctgat attccagctt atattaatat
     1561 ggggcaggtt ttaacgttcc gtaaattgga agaacgtagc cgctcgtttg ccgcttattt
     1621 gcaaaatgaa ttacgcctga aaaaaggtga tcgaatcgca ttgatgatgc cgaatttatt
     1681 gcaatatccg atcgctttat ttggcgcatt acgtgccggg cttgtggtgg tgaatgtcaa
     1741 cccgctttat acgccgcgtg aattagaaca tcaattaaat gacagtggtg cgaaagcgat
     1801 tgtgatagtc tcgaacttcg cggctacctt agaaaaaatt gtctttaata ctgagattaa
     1861 gcacgttatt ttgacccgaa tgggcgacca actttcattc ggcaaacgca ccttagtcaa
     1921 ttttgtggtg aaatacatca aaaaattagt gccgaaatat aaattgccac atgcggtcag
     1981 cttccgtgaa gccttaagca ttggcaaaca gcgtcaatat gtaaaaccga atattgtcag
     2041 ccaagatctc gcctttttac aatacactgg cggtactaca ggtgtagcga aaggggcaat
     2101 gctgactcat caaaacttaa ttgctaatat gatgcaggca aaatggattg ttgagccatt
     2161 gttgggtaat tctgccaata tgattggatt ggtaccttta ccgctgtatc acgtcttagc
     2221 actttcgatg aattgcttgc tctttatcga attaggactt accggattat taattactaa
     2281 tccgcgcgat attccaggct ttattaaaga attaaaaaag tacaaagttt cagtgattac
     2341 tggggtaaat acattgttta atgcattact taatcacgaa agttttaaag aggttgattt
     2401 ttcacaccta aaattaagtg taggtggcgg tgcagcgatc caatctagcg tggcaaaacg
     2461 ctggcacgat ttaaccggcg tacatattat cgaaggttat ggaatgacag aatgttctcc
     2521 gctgattagt gcaacccgtg gcgattcggt ggaatattcc ggctctattg gcgtgccagt
     2581 gccaaatact gacattcgta ttgtggatga tgaaggcaaa gaagtaccac tcggcgaacg
     2641 gggcgagctg gtggtgaaag ggccgcaagt aatgcaaggc tattggaacc gtcctgatga
     2701 aacggcacaa gtgctacaag atggttggat ggcaaccggt gacattgtgg taatggggga
     2761 tgatctgaac ttacgcattg ttgatcgtaa aaaagatatg attattgtct ccggttttaa
     2821 tgtgtaccca accgaaattg aagaagtgat ttcccaaaac ccaaaagtga atgaagtagt
     2881 ggcgattggt gtgccgagtg aggcttccgg cgaaagcatt aaaatttttg taaccaaaaa
     2941 agatgaaagt ttaactcgag atgaattgag aacctattgt cgtcaattct taaccggcta
     3001 caaaattcca agagacatcg aattccgtga cgaattacca aaaagtaatg tcggcaaaat
     3061 tcttcgccga gtgttaagag acgaagaatt agcaaaggtg aataaggctt aaattacact
     3121 aattaaggag cttaaaatga aaattgttga agtaaaacac ccacttgtaa agcataaatt
     3181 gggcttaatg cgtgaagcgg aaatcagcac caaagatttc cgtgagctag cgaatgaaat
     3241 tggtagtctg cttacttacg aagcaaccaa agatctggaa atggaaagtg tggagatcaa
     3301 tggctggagc ggagagaaaa ttgcggttga gcgaattaaa ggcaaaaaag tgactgttgt
     3361 gccgattctg cgtgctggct taggtatgat ggacggtgtg ttagagcata ttccaagtgc
     3421 cagaattagt gtggttggga tttatcgtga cgaagaaacc ttaaagcccg tgccgtattt
     3481 ctccaaacta gccaacgatg ttgatgagcg tttagccatt atcaccgacc caatgcttgc
     3541 aaccggtggc tcaatggtag caaccattga cctcctgaaa aaagcaggct gtaagcagat
     3601 taaagtgcta gtgttagttg ccgctccaga ggggattaaa gtattagaag ccgcccaccc
     3661 agatgttgaa ctctacaccg cctcaatcga tagtcactta aacgaaaacg gctatatcat
     3721 tccaggctta ggcgatgcag gggataaaat tttcggcacc aagtaacatc aaaactaagt
     3781 aacagcaaaa caagtaaagc tgatttaaac aagcggccat ttttcactaa agatttgcaa
     3841 atcttggtag aaaaatgacc gcttgtaatt tattgattag caatcttcat caaccaattc
     3901 ttcggttgta tcaattgccg gcgggttgtc gtgtagccag ttagccaaac gcacataatc
     3961 ggcaatggta aggttttccg ctcgggcggt taaatcaacg cctaaggcgt ccagttgctc
     4021 cgcactaaac agggttgaaa gagcattgcg taaggtttta cggcgttggt taaaggcttg
     4081 agtcgtgacc cgattgagcc aatacacatc ttttgccgga taaggcagtt ggttataagg
     4141 cactaaacgc actaccgcag aatcgacttt tggtgccggt ttaaaggcgg ttggcggcac
     4201 ttccagcacc ggaattactt ggcaatagta ttgagccata atggttaaac gcccgtatgc
     4261 tttactgttt ggcgcggcac ataaacgttt caccacctct ttttgcaaca taaagtgcat
     4321 atcttgaatt aagtcgtgaa aggtcagcag gtggaatatc aagggcgtag aaatgttgta
     4381 aggtaagtta ccaaacacac gcaccgagtc ttcgtctaag ttgagttggt taaaatagtc
     4441 acggaaatta aagcgtaggg catcttgctc aataatggtg agtttttggt ttaaaaacgg
     4501 gtggtggcgt aaacgctctg ctaaatcacg gtcaatttca accaccgtga gtttatctac
     4561 caattcggca accggctcgg ttaatgctcc taaacccgga ccgatttcta ataaaaattg
     4621 tccgtttttc gggttaatgg ctgaaactat gctgtgaata atgttcatat cgtgcaaaaa
     4681 gttttgcccg aaacgcttac gagcggtatg acctaaatgt tttttggaat ttgtagaact
     4741 cattgttatc ctttttgaaa taagaaaaga accccgcatt atgcgaggtt cttaaaaatt
     4801 ttaattattg accgacatat tgaatattgg cggtttttct aagcgctttt acccaatctt
     4861 tagaggcttc ttcggcttgc ttgttcacca gttgctgata agcacgttgg tggtaagcat
     4921 cttcggttct gtctccgtta cgagtgtcgg tgacttttaa aatatgccaa ccaaattgcg
     4981 atttaaacgg agcagaaatt tggtttggtt tgatttttga tgcaacattc gcaaatgccg
     5041 gatcgtaaat ttctaagaag ttaaaaccta aatctccacc gtctgcccct gaggcataat
     5101 caacagaatg ggtttttgcc gcttcttcaa agctgatttt gcctgagtta atatcagtag
     5161 ttaagctgct aagttttgct ttggcttgag catcatttaa aatcgggttg gttttgagca
     5221 aaatatgact aatgcgatgc tctttgccag aaacggtttt caatttgcct gctgctttat
     5281 ctttcgccat taactctttc gccagagctt gcacttgctg tggtgcaacc tgaatcgatt
     5341 tgctaatgct gatatggcga acttgttcca ttgccatttg ttgagcaata ttacgtttga
     5401 attgttcaag tgtaatacct tgataatcta acgcatctaa taactgaccg taagtaatgc
     5461 cgttttgaat tgctacattt tcaaccgctt ggtttaccgc agctgggctg actttaacgc
     5521 cggcttcttt cattgcctgt tgcaccaaca tatcatcaat cacttgatca atagccgcac
     5581 gttgagcagt ttctgtcgcc tttttcttgc caagcacacg ttgaacctgg ctttgcataa
     5641 taggggtatc attcactaac gccaccactc tttcttcaaa tgcgtgggcg gtttgggtaa
     5701 gactcattgt tgcaacggca acagcaaata atgatttgac taatttcatt tttataacaa
     5761 tcctaatgga ggtaattaag cagtaacttt tccttagaga taagtttagc gaaaaggttc
     5821 acttaattta agcactttct ttattggcag caggaactaa gaccacttgg ttaatcccat
     5881 catacgagag cgtttgcacc tgaatttgct cgtttttagc cgttggaata ttttttccga
     5941 cataatcggc tcggataggc aattcccgat gacctctatc gacaaaaatc accagttcaa
     6001 ttctggcagc tctgccaaaa tctaagagtg catcaagtgc cgcccgaatg gttctgccgg
     6061 taaacagaac atcatcaacc aaaatcactt ttttaccgtt cagatttagc tgattatcgg
     6121 ctcccataaa tacgggatct tgataaatta agtctaggtc atcacggtag aacgtaatat
     6181 ctaacgccat ataaggcaag cgtgtttgag ctaaatcttc aatgcgtttt tggattaact
     6241 cggcaatttc agctcctcga cgtttgatgc cgacaatcac tacatcttgc aaatcaccgt
     6301 gtttttcaat aatttgatgt gaaatacgag atattgtgcg ctgaaattgc tcggtatcaa
     6361 taatgatttt ttctctcgcc attcgcttat taccattata gtgcttgaat tttcttgatc
     6421 acattagtgg tagaacagcc gttttcaaag ttcaatactc gcacatcgcc gccgtttgcc
     6481 catacttctt ggctgccggc aatctcttcc ggcttgtaat caccaccttt taccaataaa
     6541 tcaggtagga tttcactgat taaacgttgt ggtgtatctt catcaaaagg cacaacccaa
     6601 tctacagagg ctaaaccagc taaaactgcc atacgggcag ctaaatcatt aatcgggcga
     6661 gcttcgcctt ttaggcgttt taccgaagca tcggtattga cggctacaat taatcggtcg
     6721 cctaatttac gggcgttttc caaataggaa acgtgaccgg ggtgaagaat atcaaagcaa
     6781 ccgttcgtca tcacgatttt ttcgccacgt tttttcgctt cttccacaat cgcttttaat
     6841 tcggcttcag aaaccacgcc aaaaccggtg gcagtgcgtt ggtgaatcgc attttccagc
     6901 tcaaccgggc tgacggttga agtccctaat ttacctacga ctacaccggc ggcagcattg
     6961 gctaaatagc aggcttcttc ataggctctg ccatcggcaa tcgcagtggc taatacactg
     7021 attacggtat cgcctgcacc ggttacatca taaacctctt tggcttgggt cggcagatga
     7081 taaggctcgt ggttcgggcg aagtaaggtc attccttttt ccgaacgggt aactaatagt
     7141 gcagataaat catagtcggc aatcagtttt aaccctttgc tgacaatctc atcctcatct
     7201 ttacaatgcc caaccaccgc ttcaaattcc gacatatttg gtgtaagcaa tgttgcccca
     7261 cgatagcgtt caaagtctgt gcctttcggg tcgatcagca ccggcacctt agcattgcgg
     7321 gcaatttgga tcatttgttg caccgattcc aatgtgcctt tgccgtaatc cgagagaatt
     7381 aatgctccgt aagcggtaat ttcttgcgaa agttttgcaa gtagggcggt agaatctaca
     7441 ttgtgaaaac cctcttcaaa atccaaccgc aataattgct gatggcggga aagaatccgc
     7501 aattttgtga tggttgggtg cgaattaatc accacacaat gattttggat ctggtgggaa
     7561 ttaagtaatt tatctaaagc acgcccggca tcatcatcgc caattaagcc gtgtaatgtt
     7621 accggtacgc caaggctggc gatgttcatc gccacgtttg ccgcaccgcc ggcacgctct
     7681 tcaatatctt gtactttcac caccggcaca ggggcttcag gcgaaattcg attggttgcc
     7741 ccgaaccagt aacggtctaa cattacatcg ccaagcacca gcactttagc attattaaat
     7801 tttggagagt attgcatcat catagttaat attctctgtt aaatgatggc gcaagcgtcc
     7861 acgcttgtgc cattattagt tttagcacaa gcgtggatgc ttgcgctatc gttagatatt
     7921 atttcttact tttcttaata aacttcatca aacgtttacg tttgcgtaat tggcttggtg
     7981 tcagtttgtt tttcttaccg gcaaaaggat tattgccttc ttggaataaa atccgaattg
     8041 gcgagccgat aatttttaaa cttttacgga aataattact taaataacgt ttgtaagaat
     8101 ccgctaattt ttcaacctga ttgccgtgaa tcacaataat tggtgggttg tagccacccg
     8161 ggtgagcata ttttaatttc actcggcgac cattaaccaa tggtggttgg tgttcatcag
     8221 cagccatacg caaaatgcga gtcagcattg aggtcgaggt tttttgcgtt gcacaagcgt
     8281 aggcttcttt tactgaatca aacaaattcc ccacgcctga gccgtgcaat gccgagataa
     8341 aatgcactct ggcaaaatca ataaaatcta aacggcgatc taactctgat ttcacttgat
     8401 ctttaatatc ttgcgacaag ccgtcccatt tgtttaccac aatcaccaat gaacgaccag
     8461 catttagaat aaagccgagt agtgataaat cctgatcgga aataccttct cgggcctcaa
     8521 tagttaatag caccacgttt gcatcttgaa tggcttgcaa ggttttaatt accgagaatt
     8581 tctcaaccgc taaattcact ttgccacgtt tacgcacacc ggcggtatca atgatggtat
     8641 attgctgacc gtcacgctcc attggaatat aaatagaatc acgggttgtt cctggcatat
     8701 cataaactac cacacgctct tcgcctaaaa tgcggttggt cagtgttgat ttaccaacat
     8761 tcggacgacc gacaatcgca attttgatat ttttatcttc aatcgactct tctgcttctt
     8821 ctgccaaggc ttcatctaat aatgccgcat cttcttcgtt ctcaaagtcg aattcggtat
     8881 cccattcgtc tgtttcttca tttgcaacat ttttagcatt ttcgcccgct tgttcttggt
     8941 taaattgctc ggcatttagc tgttcgccta aaggagctaa cacttgctcg ataagctgcg
     9001 taaccccacg cccttgtgct gcagcaattt gttctacttc gcctaagcct aattgataga
     9061 attcggcaca gtgagagtcg gcatcaatac catcggtttt attggcaacc accaccgtgg
     9121 ttttttcacg ttggcgtaaa tattgagcaa tgccgacatc ggcaggtaat aatccggctc
     9181 gggcatcaac caagaaaagc accacatcgg cttcttcaat cgccaataaa gattgttccg
     9241 ccattttttc ttccacgcct tcctctgtac catcaatacc accggtgtcg attacaatga
     9301 aatcgtagcc ggcaatatta gcgtgaccat atttacggtc tcgggttaag ccggggaaat
     9361 ctgctactaa ggcatctcga gtacgggtta aacggttaaa taaagtcgat ttgccgacat
     9421 taggtctgcc cactagggca acaacaggag tcattttgta tcctctatat ccggttattt
     9481 gcaaaatagt tgcaaaaatt ggcttattat agcggaaatt tggggaagtt gaaattagaa
     9541 gaagatcggg aacacgggga gggacgcacg gcgtgcgtcc gcaagcggtt aaaaatgttc
     9601 aattttttgc aaatatgcct aaaagaatgt gctgagcatc attcttctag ccgtaatgct
     9661 cttgaaaaac tttgtaagcc ggtggagttg ccactttttg ccattgcttg taacgcttgg
     9721 gttggggcgt gtagtaactg gttggtgagt ttgtagctta gctcacttaa tactttttgg
     9781 ctgtcttgcc cttgactgat tgcttgtagg gctttctcca acaattcttg gcgggtttgc
     9841 tccgcatctt gacgatagcg tttgattaaa tcactagact gctgttgttt taaccattca
     9901 aaaaatgctt tgctttcctc tttgacgatt tcagcggctt gtttggcggc ttcttcacgc
     9961 tgagccatat tgcgttggat aatattttgt aaatcatcaa cactataagc gtaaacgccc
    10021 tctaagtcgc cggctttttc gtcaatatca cgaggaacgg caatatcaat cagcaaaagt
    10081 gggtcaaatt ggcgttgttt ttgcgcaatt tccaccattt ctttactgat tagggtatca
    10141 gggctaccgg tggagctaat aactacatca gcttggttta aaccgagttg caaagcactt
    10201 aacgacagaa tttgcattgg agcgtttagt ttttccgcca acatttccgc ccgaatatgg
    10261 gtgcggtttg ccaacattat atttttcgcc ccgtgttgaa ttaaatagcg ggcgactaat
    10321 tcaatggttt cgcctgcccc taccagcaaa aagcgtaact tgccaaaatt atcgaaaatt
    10381 tgacgggcta aaccgcaagc tgcataagca actgacaccg cttggctgcc gatttcggtt
    10441 tcggaacgta ctcgttttgc ggtggcaaag gtgcgttgga acaagcgtga tagcttggtg
    10501 gacatcgctt tttgctgatg ttggtagaaa tcttcgctat attgataggc ttgtttaacc
    10561 tgccctaaaa tttgtggttc gcctaaaatt agtgaatcta atccgcttgc cactttcatt
    10621 aagtggttag cggcttctaa attttgttta aaatataaac attcacgcaa ctcgctttca
    10681 tcaagttgat gaatttgggc aaaccagcgg aaacattgtt cacgccattg gatattttcc
    10741 tcgctctctt cacggggaga aatattaggc tggtggaagt agagctcggt gcggttgcag
    10801 gtagagagaa tgaccacgct ttcagccaat ccgctcgttt ggatttgttc aagagccagt
    10861 ttacgtttgt catctacaaa cgccactttc tctcttaaac ccacagaggc ggttttatgg
    10921 ttgattccta aagctaaaat tgtcattgct acccacaaaa ataaagatca aaataaaaac
    10981 gcaagtattt taatcaagag cgtgaagtcg tgcaaatgat tgtgttgata ggtttgattt
    11041 atcacaaggt tgctacctct taaggagtaa agaggataag ataacttgga ttaaattttc
    11101 gattaggggt aaattttttc tgaatttcca cgtataataa aactccattt taagctgtac
    11161 aagcggtcta atttccacaa aaatttacaa ggaggcctta tggtagcaat tcgttattcc
    11221 catcaacttg aaccgaataa ttttgagttg tcgagttggt gtgcaagcct gaatatgtct
    11281 cctgtgactt ttgagcagat tcacaatgcg tggagttatg cacaggaaaa actggatacc
    11341 gactatagtc tgatgtggta tggcattgag atggttgaag tattgcacgg cttgaatatg
    11401 gacgatgaca gcttagttgc cgcaatgctt tttcctctgg taaaacacaa tgtgatcgac
    11461 ttggtgcaat taaaagaaga tttcagtaac gacatcaaaa acttagttaa aggcgtactc
    11521 gaaatggaga atatccgcca gcttagtgcc aataatgtgt cggatctgca aatcgacaat
    11581 atccgtcgca tgttgttggc gatggtggac gatttccgct gtgtggtgat taaacttgcc
    11641 gaacgaattg cctacctgcg tgatagtaac caccattccg aagaagatac cgttctcgcc
    11701 gctaaagaat gttctcacgt ttatgcaccg cttgccaatc gattgggtat cgggcagctc
    11761 aaatgggagc tggaagatta ctgtttccgc attttacacc ctgtctctta cactcaaatt
    11821 gctaaatttg aattgggcga acgccgtgtg gatcgtgagc gttatattgc aaattttgta
    11881 gcagatctga ccgcttgttt aagtcctgaa atcaagaatg tgcagattta tggacgcccg
    11941 aaacatatct acagcatttg gaagaaaatg cagaaaaaac ggttgagatt taatcagctt
    12001 ttcgatattc gggctgtgcg ggtaatagtg ccaacgttag aagattgcta cacggcatta
    12061 ggcattattc acaccaaata tacccatttg ccggaacatt tcgatgatta tattgctaac
    12121 ccaaaaccta acggttatca gtctattcat acagttgtat taggcgaagg tgaaaaaacg
    12181 attgaagtgc aaattcgtac ccagcaaatg cacgatgatg cggagctagg cgtagccgct
    12241 cactggaaat ataaagaggg ggcaaccgca gggcgttcgg gctacgaaga aaaaatcgtg
    12301 tggctacgca aattgcttga gtggcaaaac gatcttgccg attcaggcga tatggtggca
    12361 gaaatgcgtt cgcaaatttt tgatgatcgt gtttatgtct ttaccccaag aggcgaggtg
    12421 atcgatttac cgcaaaaagc tacaccactt gattttgctt atgcaattca tagtgaaatc
    12481 gggcatcgtt gcattggagc aaaagtggca gggcggattg tgccgtttac ttatcatctg
    12541 caaatgggcg atcaggttga aattatcacc caaaaagagc cgaacccaag ccgagattgg
    12601 ctcaatccga ataccggttt tgtgaataca ggcaaagctc gctcaaaaat tattgcgtgg
    12661 tttaaaaaat tagaccgaga gaaaaatatt ccaatcggca aagaggccct tgaagccgaa
    12721 atggaaaaat tcggttttac gctcaagcaa attgaagaat atgctctgcc tcgctataac
    12781 ttaaaacagt ttgatgatct ctatgccggt attggcggtg gcgatattcg tcttcatcag
    12841 ttaatgcact tcttgcaatc aaaattaatt aaaccgaccg ccgaacaaga agacgaagcg
    12901 gtacttaaac aggtgaataa aaacaccaat cagccgaagc caagcaaagg ctatgtgatt
    12961 gtagagggcg tagggaactt aatgcattcc attgcccgtt gctgccagcc gattccgggt
    13021 gatgaaatcg tgggttacat cacacaaggc agagggattt cgattcaccg tgcagattgt
    13081 gagcaactgt ttgaattaca aagtctcaac ccagaacgag ttgtggaggc ggattggggc
    13141 gaaggctata cctctggttt aagcttaacc attcgagtga ttgccaatga ccgcaacggc
    13201 ttactgcgag atgtcagtgc aattatggca aatgaaaaag taaacgtgtt aggcgtttcc
    13261 agccgaaccg atgtgaaacg tagccttgcc accattgata tggaaattca gcttaacaac
    13321 gttgaaattt tgaataaatt attggctcgt atcgctcaat tagatgatgt tatcgaagca
    13381 aaacggcttt cgagttaatc tacaagcggt tgtttttgct aaacaattta caaacaacga
    13441 atgagtatcc gcataattac ggcgtagggg cgaaaaatct ttcgccctta tcagtcatta
    13501 gataaagaga aacaaaatga aaaaattagt gattttaggt tcaacaggtt ctatcggtaa
    13561 aagcacttta tcggtagttg agcataaccc aaacccaaac caatatgagg tgtttgcgtt
    13621 agttggtgga aagaatgttg aattaatgct ggagcaatgt cgtcaatatc agccgacttt
    13681 tgcggcaatg gatgatgaaa aatccgcaca agcattaaaa acggcattgg caactttggg
    13741 gtctaaaact gaagtgttag ccggtcaagc agctatttgt gagcttgctg cacattccga
    13801 agtggatatg gtaatggctg ctattgtagg agcagcaggg ttattaccga cactttctgc
    13861 ggtgaaagcg ggtaaaaccg tattgttggc aaataaagaa tcgctggtaa cctgtgggca
    13921 aatttttatt gatgaggctc gtaaatccgg tgcgaagctc ttaccggtag atagtgaaca
    13981 caatgcgatt ttccaatcgc ttccaccgga ggcacagcaa aaagtcgggt tttgtccact
    14041 cgctgaactg ggtgtcagta aaattatttt aacaggatcg ggtggaccgt tccgtactaa
    14101 gccattaaat gaatttgatg cgattacacc ggctcaagca gttgctcacc ccaattggtc
    14161 tatgggtaag aaaatttcag tcgattcagc cacaatgatg aacaaaggct tggagtatat
    14221 tgaagcccgc tggttgttta atgcaacagc cgaggaaatg gatatcatca ttcacccaca
    14281 atcaattatt cattcgatgg tgcgttatat tgatggctcg gtgattgctc aaatgggcaa
    14341 cccggatatg tgtacgccga ttgctcacac gatggcatat ccaaaccgaa ttaatgctgg
    14401 tgtgccacca ttagatttct ttaaattaaa agagctaacc tttatcgagc cggattttgc
    14461 ccgttacccg aacttaaaat tagcgattga ggcttttgca gaaggacagt atgcgacaac
    14521 agcgatgaat gccgccaatg aaattgccgt tgaggcgttt ttaaacgagc aaatccgctt
    14581 tacggatatt gtgaacgtga atagaacggt ggtggaaaat attgctccaa ttcaagtcag
    14641 tgaaattgct gatgtattgc atattgataa actcgccaga gagttagcca aacaagctgt
    14701 tcttcagctc taatgaaaag aaaatgccta caaatttgta ggcatttttc acatattagt
    14761 tagctttttt actgattgtt accccggtaa atccaaaaat taaggtaagg attaaacaga
    14821 tataacagaa gaatgcgtat ggtagataat ctaacaccgg tacatttaat acgctactga
    14881 taaacacacc gcacacgccc caagggacta atgggttgat taccgtgcct gcatcttcaa
    14941 tggtgcgaga taagtttttc acttctaagc ctaaacgttc ataagttgag cggaaagtat
    15001 tacctgataa caataagctc aaatattgct caccgattaa gacgttaatt cccaccgcag
    15061 tgctggcaac tacaagagta acacgtcctg tgctggttaa aaagccgcta atgccgttaa
    15121 gtagtgccgg taaaatgccg aggatttgta aaagaccacc taaacttaac gcaagcaata
    15181 caatagtgat ggtaaagaac atactattaa tgccaccacg agagactaat gatgccactt
    15241 cgcctaaatc tacgccttca acaggtttat agctggtaaa gaaataattc cctagctgtt
    15301 caagggttgg cgaactgtgg atataggtaa taattagcga gagtgttact gtagttacaa
    15361 taatataaat tgaattaaca cgcttaattg ccattaaaat taacgtaaca aaaggtagca
    15421 aagagtagcc gtgtattaat cctgtttctt gcaaattggt ttttaggttc tctatatttg
    15481 ctaaattttg tgcatcagta ggttcggtta aaaaccaaag gaataatgct gttaaaatcc
    15541 acgcagggat ggtggtataa agcatattac ggatatgagc gaacatatca atgcccacta
    15601 cggaagcagc aatggtagta gtgtctgaaa gtggagaggt tttatcgcca aaaaacgccc
    15661 ctgaaaccac cgcacctgct acaatcgcag ggttcgcacc gaaagcagaa gccataccga
    15721 taaaagcaac accgatggtg gcggttgtgg ttaaactact gcctaacgca atacctacta
    15781 cagaacttaa tgcgaatgca gaaatgtaaa ataattcagg tgaaatcaca cttaagccat
    15841 aatacattaa ggttggaatc gcaccagaca tcatcaaagc tgaaaccaat aatccaataa
    15901 aaaagaatag gtaaaccgca ccaatactgg acatcagccc gcttgccata ccctcttgca
    15961 tatttttaaa cggaatttta ttgattaagc cgaaaagtag cagcattaca atcacggcta
    16021 gaattgaaat ttgaggcacc cagccaagcc caatcattgt aaaacctaag ataaagaccg
    16081 tcaaaagcga aagtatgacc gctgtcattg gttttaattt aagtaggtag ttattcattt
    16141 atactcctaa tttattattt ttaaataagt tggctattat agactaattt tgttcatcta
    16201 agtagtgcgc gcctaaactc tgctcattta gcatttgatt gaccactaat tgagcggttt
    16261 ttaacgcatt gtaaatttca atgccttgat attgagcgtg atagattggg tcaaattctt
    16321 gttccaaacg gtttaattcc tcaagtagta gtttgaggtt gctccgagtg cggtacacat
    16381 tggcgtataa cgccattttc tctcgaatag tagaaagcac ttcactggca gttagttcta
    16441 gattagcagt tgaattagca agctgattta atgctttttc tgccattgca agcggtgaga
    16501 tttcgctgtt tttttgcaaa tcattctgtt ctagcgaacg aacaatttcc gataccgcac
    16561 gtttggcaaa cactaagcca ccgccgactg agttaccacc catacggttt gccccttcaa
    16621 tgcagtgaga aatctcacca atcgcaaata gcccttttac tgcactttcg ccccattgat
    16681 taatttgaat gccgccgtta cagctatgag caaacggctt gattgcaatc ttatcttgca
    16741 ctaaatcaat cccgacttca tttttgagcc aattcaaata gacggtataa aactcttccg
    16801 tgtcttcata cagtttgggg ctgtatttaa ggtaaatgcc tttttcgtga gggttttcaa
    16861 gcaaatgttt catcatcact aaatcgaact ccacgccggc aaaatcaagg ctgaaaggag
    16921 cataatggct acgttccagc accatttttg caaattgctc ggcatttaag tgtggaaata
    16981 gattgttacc cttagaatcg gtgatttggg tgacatattt caatgtatgt tcgccaaata
    17041 gcaccttgta ttttggctcg acaaaggcag gaatgaactg aataaaggca agattaacca
    17101 aggttgcccc ggcttgttgt gccacataat gaagtgaacc gataacatca gccgggtaga
    17161 gattatcttt atacagccca gcaatcccgc ctgcggctaa aataagatgc ttggttttgc
    17221 aaaaaatata ggaaatttga ccgcttgtag tgagcgaaaa taccgcacct tgcacttggt
    17281 tattgtctgt gacgatgtgt aaaagggtag cattttcata gaaagcaatg tgttgctctt
    17341 gcaaaatctg tttcgctcgt agggcagatt cccgccaatc tttaattaaa taaattgggc
    17401 gagggtattt ggcaaaacaa gccggacggt tgtcgttacg cttccaaggc tgaaagccga
    17461 tacggtggag tagttcaatc gcttgtggtg aatcttcgat ataggcttgt accacattag
    17521 ggttgatttt gcctttgccg atgttttcaa tatcttcttt aaagtgaatt ttatcggctt
    17581 catcgccggt tacttgaatg ccaagtgttg ccttaagcgg aaagtagctc gctcccgagc
    17641 caatcgtaga tttgctaatc agtaaggtat taatgccttt ttcttttgcc tcaactgcag
    17701 cagaaagccc agcgatgcca gagccgacaa ttaataaatc aatgttaatg gtttgggtaa
    17761 tttggatcat aaataaaaac acaagcggta aaatttgcga aaaattttac cgcttgtagg
    17821 cacaaagcac aattataata aagatggtgc tgcttcaatt aaggttcggg tgtactcttg
    17881 cgttgggcgt tcaaatacgt ctgaagcatc gcccatttcc acaattttac cgccgttcat
    17941 aaccacaatg cggtctgaaa cttggtgaac ggtttgtaag tcgtgagaga taaataccat
    18001 cgctaaattg agttcttttt tcaagtctgc cagcaggttc agtacttgag ctcgaaccga
    18061 tacgtcaagt gcagaggttg gttcatcagc cacaatcagc ttcggattta atgccaatgc
    18121 acgagcaatc gctacacgtt gcttttgccc accagataat cgggttggct cgaccaacgc
    18181 cgctgaacga ggtagaccga ctcgagagag caaatcataa acccgcttct cacgttcttg
    18241 cggttgtaag atattatgaa tatccatcgg atcttttaaa atatccaaca cacgcatacg
    18301 cggatttagg gcggttgccg ggtcttggaa aattactgac acttggctac cgaaatattt
    18361 gcgagcttcc gcactgccat atttcatctc taagccgtta aattttactg tgccggaggt
    18421 tggtttttga agcccgatca tcacactggc taaagtagat ttgccacaac cagattgtcc
    18481 aaccagaccg actgtttcac cggcgtgaat tgctaggctg acatcattta ccgccgtgaa
    18541 ttttttcggg ttaaataagg tgccgtcacg agaagcaaat tgcacaacaa tattagacag
    18601 ttcaataatt ggctgttctt tgataaattt catactcatc gtattgctct cctttatgct
    18661 tctttagatt gtggatcttc taagtgtgaa gcccagaagt gttgtgggtg atcaccggtt
    18721 ggcactaatt tcaagtgctg ttcagggttt gcatctggtc ttaatgaacg actcgcaaaa
    18781 cggtcacctt tcgcaaaatc aaatggtgaa ggcacactgc ccggaatttg gtataggcgt
    18841 tctgcacgca attcggtaga aagtaccgag cctaataatc cacgtgtgta ttcgtgtgtt
    18901 gggtttgcca ataatggcgt agttggtgca gtttcaacca cttgaccagc atacattacc
    18961 gtgatgtggt gagcgatttg tgccactaaa gctaagtcgt ggctcacgaa taccattgca
    19021 aatccaagtt tctcacgtaa ttcgtttaat aatttgatca cttcagcttg aacggttacg
    19081 tctaatgctg ttgttggttc atcagcaatc aatagtttcg gttcacgagc aagtgccatt
    19141 gcaattaata cacgctgacg ttgtcctcca gataactcgt gaggataacg gttgagcgtt
    19201 ttttccgggt caagttttac ccattgcaat aatgtttctg ctgattgttt accaccacgg
    19261 ctgattaatt gtgccatttg gtctttaata cgcattgatg ggtttaacgc acttaatgca
    19321 tcttggtaaa tcatcgagat ttcgtgacca cgaagggcat ttaaatcggc tgattctaaa
    19381 agattgtgtt gtttacctga acggtcggta aagaggattt caccagtgat ttttgcagtt
    19441 tttggtaata aacccataat tgagaatgcg gtgattgatt taccacaacc agattcaccc
    19501 actaagccca tggtttcgcc ttcgtgaaca gtaaagctga tattatcaac aagcggagtt
    19561 tcaccataac gatttgggaa acggattgat aagtttttca cttgtaaaat cggtttggcc
    19621 gttgagttta attgcatacg gtcagtacgg gtcgtttctt tctcgtgtaa tttgagtaag
    19681 taacgtttta acgcaagagt ttcagacatt gcttcttgaa cgtctgttgc caatggtttt
    19741 gcgacatctt ctttactttg agttgaacgt tttagttttg gatttactaa cgcatcggtt
    19801 aagccttcag caaggatatt taacgcaagt acggttaata aaatcataat ccctgcgaaa
    19861 gttgtagccc accagaagcc acttaataca aggttacgac cttcagataa gatatttccc
    19921 caagatggga atggaggctg gacacctgca cctaagaatg aaagtgatgc ttcaaatacg
    19981 atggcatcag caaccatcac tgttgcgaat actaaaaccg gtgcagcagt attacgcaca
    20041 acgtgtttta atagaatata agtacggcta ccaccaatta cacgctcggc acgcacataa
    20101 tcttcttccc attgagatac tacgttagca cgcaccactc gggcaagttg gggagtgtaa
    20161 acaaccgcaa tagtcacgat aatcacaggt acagagttac cgaaagtggc aagtaataca
    20221 gcagctaatg cgatacccgg gaatgccatt aagatatcca taaaacgcat aatcacttcg
    20281 ttaccgaatt tatcggcggt tgccgcagtt gcccctaaaa tactaccgaa tagaatcgca
    20341 acgcctaccg cacctaaacc gataaagaga gatgttcttg ctccccatac catacgcgag
    20401 aaaatatcac gtcctaaacg gtctgtaccg aataaataat caccgcttgg agcttgcact
    20461 ggacgtagcg tttgcagtgg gtcataaggc gctactaatg gtgcaaaaat agcgactaat
    20521 gcgataaata caataaagag taaggcgatt tttgagctgg ttgataatgc tctaaatctt
    20581 gcaccgcctg aagcaagtcg agcagctaat ccttggcgaa acatattata aactccgaat
    20641 ttttggatta atgagcaagt aaagaatatc aacaatgata ttcaccaaca cgaaagtaaa
    20701 tgcgatagtt aatactacac cttgcactaa gttcaagtcg tgattgacga taccgttgaa
    20761 aattaattta cccattcccg gtaagtcgaa gatctgttca atcacaactg caccacctaa
    20821 taaataacca acccgtaaac ctaatactgt taccggagta attaaggcat tacgaagcac
    20881 gttgtggcga atcaccgttg agtaaggtac accattaccg atggctgttc gaacataatc
    20941 tttatccatt tcttctacca ttgttgtacg cacgacacgg atcaatgagg cacatacagg
    21001 aacagctaaa gcgagtgaag ggagaaccat tgagtttaca tagccttttg gatcttcgct
    21061 aaatggaata aaaccgcctg aaggcaacca atctaatttt aaggagaagt attgaatcaa
    21121 taaaataccc aaccagaaag aaggtgtggc aacagcggca accgagatta aacggattaa
    21181 ttgatcaatc caagtatcac ggtaaagtgc tgccgttaca cctaatgtga aagagacgat
    21241 agcagctaag aagaccccaa taaaggtaag ctgtaatgtt aaagggaatg ctttagcaat
    21301 catatcaaca ataggctgtt ctggaggggt tgtcataccg aaatctaata cgaataaatt
    21361 ccctaaaaag cgaaaatatt gtacaagcaa tggatcattt aacccatgtt gttcacgata
    21421 gatttcttta gcggcttcgc tggcgctttc accgagagca acggttgccg gatctccggg
    21481 ggtgaattgt aaaacaataa ataccaaggc agttacgcct aagatcatta ccggcagagc
    21541 cattaggcga cgccataata atcggagtat aatttccatt ttcttctcct tgagtgtagt
    21601 tacactccaa aattaaatct aagtttacct acaagctaat atagtgatat gtaggattat
    21661 aaatgaaaat atagaaatgg taagaattct gagtgatata agacgaatat tacatcattt
    21721 tgatttttat ttatgcaagc ggtaaatttt ttgtaaaata ttaccgcttg catttagcta
    21781 gccgttacga ctatttagca cgaccaacac cgataaatga catacctgtt gttggtaatg
    21841 gttggaaacc tgttaatgcc ttatcattcc acgcagttgg caatttacgg tgaataattg
    21901 ggtaaagagg cacttgttca gcgataatat tgattgcttt cgcccaggct tctttagaag
    21961 cagcaggatt tttggccgct tcatcaagca gtttttgtac ttctgcatat tcagctgttt
    22021 cagaccagcg gaaacgacgt ttcggccata catcaccacg ataccaccag cttaataata
    22081 ggtctaagtc attaccgaaa actgatgggt cgccaggtgc aatcacaact tcgtatgcac
    22141 ctttatcaac gtgtgtgcca tataatgcac ctgattgtaa gtgtttgagt gaaactttca
    22201 cgccttttaa gctgttccac gattcaagaa ttaatggcgc acattctttc acccaagagt
    22261 gatcggttgc caacaattca aattttaact ctgtcacacc cgcttctgct aataacgctt
    22321 cggctttcgc tttatcgaaa tcatattgcg aatttgcttt cacataatcc gggtgagtat
    22381 cttgcacgta agatgtagca gcttttgcat tgcctaagaa caccacatca attaattttt
    22441 gggtatctaa gccataatgt aacgcttggc gaactttcgg gttgttaaac ggtgcttttt
    22501 cacagttaaa cattaagaac agtaagccga atgattgtac tgactcaacg gtacctttac
    22561 gttttaagcg ttctgcatct aagtaaggca cagactcaat tgcttgaaca cggccagatt
    22621 cttgagcggt tacacgagcg gcatcatcag aaagtaagaa ccaagtcatt ttctcgactt
    22681 gtgccgggta gccaccgtta taatctgcaa atgcttcgaa aacaatacga tcatctttag
    22741 ttgcggatac aaatttatat gggccagtcc ccactggatt agcatcaaat gcagcttgac
    22801 cggcttctac cactgctttt ggtacgattt taacaatggt taggcgctct ttgaataacg
    22861 caaatggata tttcaatttg aattcaacca ctttgtcatc aacttttttc acagtgtcaa
    22921 taaatggaat gaattgtgcg aaaagtgatg ctttagctgg gtctaataca cgttcaaatg
    22981 agtaaactac gtcttcagtg gtaaccggat taccattgtg gaatttagcc ccgtcacgta
    23041 aagtaattcg ataggttaca tcatcaattt tttcaggctc tgctgctgcc aatgctaaat
    23101 atggtttgcg agtggctgga tgtaagtcca cgaggccttc aaaaatatgg aggtttgccg
    23161 ccattgagga tgcaccgctt gaggtaagcg ggtcaaatcc ggttgatatg ggataagcta
    23221 taccggcctc aatcgttgag ccggcggcag gggctgcaaa tgctttaggt gtaaatgtac
    23281 ctaaagtgcc actaaatgcc ataccggcac cgacacctgc aattagtttc ataaaaccac
    23341 ggcgggattc gttgtgttca aactgttttt tcatcttaaa gctcctttaa gtttttatga
    23401 taggggcggt attatttgct gaagtgaatt tatcaaaaaa tgcacttttt tgaattgaat
    23461 ttatcatatc attaatgtca aagtggtaaa gcaagaatta ttgaatttgt gatctagctc
    23521 acgaaaatct ttgcttttca cttcacgtgc ttttcgttat tattcgccaa tttaatcata
    23581 aatgaaaaat tgacagtgaa cctgatttta ccgcctttaa gcctttatat ccatatccct
    23641 tggtgcgtgc aaaaatgccc ttattgcgat tttaactcgc acgctcaaaa aggattaatt
    23701 cccgagcaag aatatatcga acatttactt gcagatttat cccaagattt gaccgcttac
    23761 cgagtggcaa taggtgatcg taaattatat tctattttta ttggcggcgg caccccgagc
    23821 ttattttcag cggaagggat agcttattta ttaattgaag tagaaaaacg gattgcattc
    23881 gagccaaata tcgaaattac actggaagcg aacccgggaa ccgcagaggc agagcgattt
    23941 ttaggttacg cacaaggtgg cgtaacccgc atttcaatgg gaatccaaag ttttgagccg
    24001 gaaaaattat tgaaattagg acggattcac gatgcaaatg aagcaaaaca agcgattaaa
    24061 tttgcccaaa attctgcaaa atcgggcttg caaagtttca acattgattt aatgcacggc
    24121 ttaccaaacc aatcggttaa acaagccttg gcggatttag aaacagggat ttcactcaat
    24181 ccaccgcatt tatcgtggta tcagctcacg attgagccga atacgatgtt ctattatcgt
    24241 aagccaacct tgcctgatga tgacgagtta tgggatattt tcgagcaagg gcatcaactt
    24301 ttaacgaatg ccggctatga gcagtatgaa acctcggctt atgccaaaaa aggctaccaa
    24361 tgcagacaca atttaaatta ttggcgattt ggggattatt tggcgattgg ttgtggcgct
    24421 cacggcaaaa tttcatatcc gaccggggaa atttatcgct ttagcaaaat caaacacccg
    24481 aaaggttata tgcgaggtga atatcgctac agccaagatt tgattgaatt agacgaccgc
    24541 ccgtttgagt tttttatgaa ccgctttaga ttacttgaag cgacaccaaa acaagaattt
    24601 gaatggttta cagggttaga cagggaagtt gttcgcccga caatcgattg ggcgttaaac
    24661 caaaactata tttccgaaac cgcaacccat tggcaaatta cccaacacgg taaattgttt
    24721 ttaaatgaat tactggaagg atttttggaa taatttgcaa aaaaacgact aaaaaaagac
    24781 cgcattgtaa tgctaaatta caggcgtttt ttgtgatctg cgtcgaaaaa tcctcatttt
    24841 ttactagaca aaaaaacagt gtttagtagc atagccttca caataaggag agcttatgat
    24901 cgataaacaa atccgtaata gtaccgccga atttttgatt tttactcaac aaaatggggc
    24961 agaaaatatt accgttcgct atgaagataa cgaaatttgg cttacgcaaa aattaattgc
    25021 tcagcttttt gattgctcaa cagataatgt ttctttgcat ttaaaaaaca tttttaatag
    25081 tagggaactc gatccaaatt cagttaccga ggatttctcg gtaactgccg cagatgtcaa
    25141 aaattatcgt accaaacact acaactttga tgccgttatt tcgataggct atagggtaaa
    25201 ctcggtgcgg gcgacacaat ttcgccaatg ggcaactaaa gtattacgga atttcgccca
    25261 aaaaggctat gtgattgacc gccaaagaat ggaaaatggt acatttttaa atgaagatta
    25321 ttttgagcag ttgctggcag aaattcgtga aatttggtta tctgaacggc gttcttgagg
    25381 gcaaaggcaa aatgcgtttt gatgaagcga aattgcacgc agaaagcgaa tttgagaaat
    25441 accgcattgt tcaagacaga ttatttgaaa gcgattttga caaggtattg aaagaaatgt
    25501 tgcgttcgga aaatcaatac cgtccatcat tttcaacaac ataaaacaac cgcattttgc
    25561 aaattttttg ccaagtttga ccgcttgccg tgtataatgc aagcggttat tttttatgga
    25621 aaaattacaa atgaatatct tacaagcagg cgataaagcc cctaaattta cccttcagaa
    25681 tcaggctgac gagccggttt ctctctctca atttgccggc aaaaaagtac tggtttattt
    25741 ttatccgaaa gctcttacac ccggttgcac tacgcaagcg tgcggactgc gtgacagcaa
    25801 atcagaactt gatgagctca atgtggttgt tctcggaatt agcaccgatt taccgaaaaa
    25861 attagctcaa tttgtagaga aaaaagcact taattttacc ttattatctg acccggatca
    25921 ccaagttgca gaagcctttg gtgtatgggg cgaaaagaaa tttatgggaa aaacctatga
    25981 cggcattcat cgcatcagtt ttttaattga tgagcagggg gggggtgagc aagtgtttga
    26041 taaatttaaa accggcgaac atcaccaaat gatcgtggat tatctacggg gtcgctaatg
    26101 ggaaaaaaag ccattttttt agaccgtgac ggcacaatta atatcgatca cggctatgtg
    26161 catcaaattg atgattttca atttattgac ggtgtcggca aggcgttaaa acagctacaa
    26221 gacaaaggtt atttattggt gctggtaacc aatcaatctg gcattgcccg aggctatttt
    26281 agcgaagcac aatttatgca actaaccgaa tggatggact ggtcgctcga tgaagattat
    26341 ggcgtggtac tagacggtat ttactactgc ccacatcacc ccgaaggcaa aggcgaatac
    26401 cgtaaagatt gtgattgccg taagcctaaa gcaggtatgt ttactcaagc gattcaagat
    26461 ttaaacatcg acccaagcca atcttatatg gtgggcgata aattagaaga tatccttgcc
    26521 gcagaagccg ccggcgtgaa aaccaaagtg ctggttcgca ctgacaaagc agtgacggaa
    26581 gagggtcagg ctaaagccga tttggtgtta gatagtttag ttgatttagt aaaatatatt
    26641 aaataaagca atgaattata acgataaatc cctttccgcc ctgaaattag ggcaaaaaac
    26701 cgagtacaaa agcgaatatg atgcctcgct tttgcaacct gttcctcgta aattaaaccg
    26761 tgatagttta ggcattacac aagagcagcc ttttaaccga ggggctgatg tgtggacttg
    26821 ctatgagcta tcttggttga accaaaatgg gctaccgcaa gtagcgattg cagatgtcgc
    26881 cattgatttt aagagcgaaa acttaattga atcgaaaagt ttcaagctct atttgaacag
    26941 ttttaaccaa accaaattta gctcaataga agaagttggg aaaacgattc aagcggatct
    27001 ggctcgctgt gcaagcggtc aggtttcggt aaaaatttac caattatcac actacacgca
    27061 acagccgata gttgattttg caggcgattg cattgatgag caagatattc aaatcgacag
    27121 ttatgcgttt tcgactcatt atttagagaa cattgcagaa gatgagttgg tggaagaaac
    27181 cttagtcagc catttgctca aatctaactg cttaattacg tcacagcctg actggggaag
    27241 tgtgcaaatt cgctacaaag gcaaaaagct aaaccgtgaa aaactgttac gttatttagt
    27301 gtcgttccgt gagcataatg agttccacga acaatgtgtg gaacgcattt tctgtgattt
    27361 aatgcaattt gccgagccgg aaaaattaac agtttacgca cgctacacca gacgaggcgg
    27421 gctagacatc aatccgttta gatcgaattt tgaggaagtg ccggaaaatc taagaatggc
    27481 acgccaatga ttttgttgtt ttttgttccc gtctgaaccg cctgagcgtg tttgaattgg
    27541 taggtaaatt tactctgagc ggagcgaatg taaatttgcc gttaagccaa ttcaaactaa
    27601 gcgaggatag cggtgaagct cgggttgcct ttcttttggt tacttttctt tggcaaagca
    27661 aagaaaagta acaaatagca gatgttgatt tttatgatga atgaatatag cccaaattac
    27721 tatataggcg taatgtccgg cacgagcctt gatggggtgg atattgcttt gctggatttt
    27781 gcaaaaaatc caccgaaaat gaccgcttgt gatttttttc caatgccgga ggaattacgg
    27841 gcggatattt ctgcgttact taaaaccggc gaaaccaatc tccaaaaact gggggaaatt
    27901 gatcaccgtt taggcttgct ttatgcagaa acgatcaatg cgtttttagc gaaaaatcag
    27961 ctaaaagcag aagatattca agccataggt tgtcacgggc agacggtgtg gcattcaccg
    28021 gattctgagt atcccttcac cacccaaatt ggcgatatga atttggtggc agagcaaacc
    28081 ggtattacca ctatcgctga ttttcggcgt aaagatatgg cagtaggtgg gcaaggggct
    28141 ccgcttgtgc cggcatttca tcaagctatt tttagcgatc caaatcgtct aacagtggtg
    28201 ctgaatattg gcggcatcag caatatttcg gtgttaaatc ccaattcccc tacagttggt
    28261 tatgatgtag gagccggaaa tactttgatg gatagttgga tcgaacaaca tcaaggcaaa
    28321 agatacgata aaaacggcga atgggcgaga acgggcaagc tcaatactga actgttagcg
    28381 acgttattag acgagtcgtt ttttgctaaa gccccgccta aaagcactgg gcgggagctg
    28441 tttaatttaa cgtggcttgc aaaaaaattg gaaaatttga ccgcttgtca gccacaagat
    28501 gtgcaacgca cgctggcaga atttactgct caaagtattg ccaatgagtt attacgcttt
    28561 gaaagctctc tgcctcgctt gcttttagtt tgtggtggtg ggtcaagaaa tccgttatta
    28621 atggaacgga tttctgcttt acttcccgag tgggaggtta caaccactac agaatttggt
    28681 ttagatattg attatgtaga ggctgccgct tttgcttggt tagcttacca gcgaatccat
    28741 aatttaccca gtaatatgcc tagcgtgaca ggagcaaaaa aagccgtgag tttgggcgtg
    28801 atttaccctg cttagattaa gaaaatgtaa ggtaatacat tcattactcc gagagtggta
    28861 atagctaaaa ttaatttaga gcttccaaac attaagcctt ggtttttacc attgtctaat
    28921 ttcactagca tattagatag tgccgtattc gcatacacgg taatagtggc aaaaatcact
    28981 aaatagagaa atgcagtgat ggttgaaagc cattggagtg cataaaacca aggcattatt
    29041 tgaagtataa tcagtattgg tgcaatataa ataagtttat gtactgtcag atttaatttt
    29101 cgtgaaaaat cagattgcaa taacactgca attaaattat tcacaaagaa tgagacagag
    29161 actaattctg gtttatccag aagattggtg aatatatagg ggaatataat ataaaatgca
    29221 cttcctagac caagcggaat aaaaagtaat aaatgtacaa aaataaattg ttcatttagt
    29281 attcctttta atgcaccaaa tttaaagcgt tcgcattgcc catttgcaca ttcatgtgtt
    29341 ttgggtgcgg ttttaaacat aacgaatagc ataataattt caatgcagca gctaatccaa
    29401 ataagccatt ctgctttacg gtagaaaata aatggaatag taagtaatgg tgctaccatc
    29461 gctgacaggc ttgatacctt cagaaattta ccttgagcct tagttttgag ttggtaacta
    29521 tctccggtat cttgaatatt ggctaataag aatgctctcg cattggtact aaataaggtg
    29581 ctaccgagcc cgaagaaaaa ggtagcaagt aatagcagag ggtaattttt tgttgtcagt
    29641 aataaaatgt aagctagggc atcgagcaaa cagccaagca tcatgatagg gtaacgtcca
    29701 aaactatcac cccaagaacc tgcaaaaagg gaaaatgctt ggctggtaaa gaccaacaaa
    29761 gaaaatgcag ttgcaatttg gctggtttca aagcctttgt agaatgtaag tagataaaaa
    29821 atacgctttg cattgcagca taagcaagac cggaaaagaa tttccgccat aaaatgatat
    29881 tttggatatt catagataat atttaatgag aaaaatgatt tctaatataa ctaaatttaa
    29941 taaggaagca caatgagaaa aatcaaatta ttgcatgtta ttttatcttt aatattaggg
    30001 ctttctgcgg cagcgattgg aagttatccg gcaataaaag agattggcga ggagcgggca
    30061 aaaaaacatc aagatatatt agatttaaat agagaacttt atagtgcaat ggtgaaccgt
    30121 gatttttcgg ctttgcaaac attactaagc gatgattttg agctttattc atcaacaggt
    30181 gaagtgattg ataagcaaga atggatcaaa ttaatccaaa agagagtatt ggaatttgat
    30241 aaggttgatt tgctatctac cgattttcag gggaatcaat catctggatt attaagagta
    30301 aatggtgagt tttttgggat agagcgggat aatgtggtgg tagaaatgaa tattcgaacc
    30361 attgtgagaa atacagaacg ccaaattaaa tgtattgtga ttaaagaggt ttaagtgtcg
    30421 caactattga atgccttagg gcagatgatt accgaacaac gtaatccgaa ttcgatgaat
    30481 attgaccgcc tttcagcgtt agatatagtc caagtcatca accaagaaga caaacaagta
    30541 gcgattgcgg tggagcagtg cctgcctcaa attgcacaag cggtggaaaa aattgtgcaa
    30601 gcctttgaga aaggtggacg attggtttat gtcggtgccg gcacgagcgg aagattaggg
    30661 gtgcttgatg cttcggaatg tccgcccact tatggcgtaa aacctgaaat ggtggttgga
    30721 ttaattgccg gtggtgagcg ggcattacgt ttcccgattg aaggggcgga agataatcgt
    30781 gagcaaggca aagtagattt acaagcggtc aattttagcg aaaaagatgt attggtcggg
    30841 attgcagcca gtgggcgaac gccttatgtg ttaggggcga tggaatatgc taagcaactt
    30901 ggtgcgacca cggtgtcgat tgccagtaac ccaaattcgg caatggcaca aattgcggat
    30961 attgcgattg aaaccgttgt cgggccggaa gtgctaaccg gttccagccg tatgaaatcc
    31021 ggcacagctc aaaaattggt attgaatatg ctcactactg cgtcgatgat tttaatcggt
    31081 aaatgctacc aaaatttaat ggtagatgtg caagcaacta atgaaaaact caaagctaga
    31141 gcaattcgga ttgtgatgca agcgacagaa tgtgaacgag aagtggctga agatacacta
    31201 aaattagccg aaaataacgc taaattggca attatgatga ttttaggtaa cttagataag
    31261 cagaaagccg aagatttatt aagccaaaat caagggcgat tacaaaaagc gttaaaatag
    31321 aagtatgtct gctctcccct tgtgggagag agacagatca atcattcatt cagaatgatt
    31381 gatcagagag aggggaggca gatttaagca aataaatggc gatgtttcac tagttcttct
    31441 ttggttaaac tgaacacccc taagccaccg tgttcaaatt ccagccagtt aaacggcaca
    31501 ctggggaatt gttcgattaa atgcaccata ctgttgccga cttcgcagac tagcacgcca
    31561 tcatcattta agtaatcagc ggcttgagca agaatccgct tggttaaatc taacccatct
    31621 ttgcccgagc caagagctaa tgccggctcg tgatggaact cttcgggcat atcgcctaag
    31681 tcttcttcat caacataagg cggattagtc acaattaagt cgtatttatc ttgcggtaaa
    31741 ttgttaaaca gatcggaaca gatcgggaaa actcggtgag acacctgatg acgctcaata
    31801 ttaatttgag caacgtctaa ggcatcaagg gaaagatcaa ccgcatcgac ttctgcttcc
    31861 ggaaaacgct cggcacaggc aatcgcaata caaccgctgc cggtacacat atctaaaatt
    31921 cgatgtggct cgtttttgat aatgccttta aacccttttt tgatcagctc accaatcggt
    31981 gaacgtggca cgatcacacg ctcatcgaca taatattcac cgccacaaaa ccaagccgag
    32041 ttggtaaggt atgctacagg tttgcgagag cctaaacgct cacagattaa attgatgata
    32101 cgttctttct caactcgagt taagcgagag gaataaagtg tttccggtac atcaaacggc
    32161 aaataaagcc ctgccaacac taattgttgt gcctcgtccc acgcattatc gtagccgtgc
    32221 ccgtaataaa gatcagaggc attaaaatag ctataccccc aacgcatcat atcttgaata
    32281 gtgactaaat cattcacaat gggcagggcg gaaatttcat caagaagttc ggtattatat
    32341 ttaaacattg gcgtgccttt tttcgtattg ttaaaatggc tgtgttatac cacattttgc
    32401 tttgtgttag aatgtaaaaa tgaataagaa ggaaaagata atgaacgata cgttattaga
    32461 cgatgaattt gcattattca gagaggcagc aaaaggcacg aaaaagctga cacaaaatac
    32521 ctttgtgccg aaagcggaac ctcgcaagaa attgcaagaa ttgcgagaat taaaagaaaa
    32581 agctgataca gaattttatt tctcggatga atatgagcct ttgctgaaag aagaaaatga
    32641 aaaagtgaaa tatctgcgag aagatgttga tccttatatt ctcaaacagt tacggcgagg
    32701 cgatttccaa cccgagctgt ttttagatct gcatggttta actcgagaaa aagcgaagaa
    32761 agagttggca gcgttgattt tagcctgtga gcgggaaaag gtatattgtg ccagtgtgat
    32821 gaccggcttt ggcactcgca cgctcaaaga tcaaattcca cgctggttgg ttcagcaccc
    32881 aaaagtaatg gcgttgcacc aggccccgaa agagtggggc ggagatgcct ctattctaat
    32941 tttggttgag cagcctgaaa atcctgaaaa aatgtttggg cgttaaaatt cggctgaatt
    33001 gccaattttt tattaaaaac gaccgcttgt gtatcattaa ggagaaatga tgtcagaaca
    33061 aactttccac caacttacag cattattaga ttcgcatcga gctcgctatc gagtggtagg
    33121 acatccttct gccgggaaat cggaagaagt ggcaaaaatt cgaggaacag aaatcgggca
    33181 aggggcgaaa gccttagttt gcaaggtaaa aggcaatggg gtaaagcaag ctgtgcttgc
    33241 tatattacct gctgattacc aagcggattt aacaaaaatt gcagagcatc ttggtgggtt
    33301 aagagcctca cttgccagcc cggctgaagt ggcagaattg accgattgtg tatttggggc
    33361 aattccgccg tttagctttc accccgattt attattggtg gctgagccaa gtctgtttga
    33421 gcgttatgct gaattagcct ttaatgccgg tacgttggaa cgttcgatta ttctgaatac
    33481 cgaggattac caaaagatag ttaatcctgt gttggttcaa ttcgccaaaa gctaggttat
    33541 ggggcgaaaa actccacgat tttgccaata gcgatatcca ttaatttagg gttagaaaaa
    33601 gcgtgatctg ccccttcaat cgggagcagt tcaatcacgt tattatcggc aaactgctgt
    33661 gaagtgctta acgccaccat ttcatcttcc gtgccgtgca gaattaaaat ttgctcggca
    33721 taatccaaat aatcgttttg ggtaatatcg gttagctcat caaaaaaggc ttggctgatt
    33781 ttcatctggc gatcaaaccc ccacaaaatt tctcgccctt tggcaagttt ttgccgttcc
    33841 tcttctgaca agcggttcat taacgaatgg tagatttgta ctgccggtgc ccgcaatgca
    33901 attttccgaa acggattgcc tttttccgcc agatatttca aggttaaata gccgccaaaa
    33961 ctagtgccgt aggcatacag atttttagca ttcaggctgg ttttggcata atcaatcacc
    34021 agttgcagat aagtaatgga ttcctcaacc gagagttttt tacgggcatc atcaccgtgg
    34081 caagggaaat caaaggcaag ggcagcgtag cctttatatt ttgagctaag gcgttcccca
    34141 aatttggtat tcgatttcag atctttagac gaaccaaagc cgtgcaacac aatcactaca
    34201 ttctcgatct gctgcaattc tttttcataa aacagtttac agcgaatgct gagaccctgt
    34261 tcattaatct caaagcgttt ttccatttta tgttaagtat tgaaattgaa gatgacaaaa
    34321 ttttagcact atttgagtgc aagcggtcgg atctctcgca aaatttgcaa attttccggt
    34381 aaatttaacc gcttgtacta gccaaaattt aagcatattt atagaataca gccttcgttt
    34441 ttaaaaggag aaatgaaatg ttacaactta ttttagcgat tttatgtagc gtgactgtag
    34501 gcgtgttgat taaattcgcc cgccaaaaag ggattcgtat cgaccaaagt attgcggtga
    34561 actatatcgt gactaccacg atgacgtttt tattactaaa accggattac caagggcagt
    34621 cgattaccga tattattgct aataacccga cttcctatat ttttgtgatt ttaagtatca
    34681 cattgcctac tattttcttg gtgcaagcaa aagccctaga gtttgccggt attatccgaa
    34741 ccgatgcggc acaacgttta tcgctgttcc tgccgatttt ggcggcattc accatttttg
    34801 gtgagcaggt tacgcaaaat aaattaattg cacttctgtt agctttcgtg gcactcggtt
    34861 gcttattatg gaaaggcaat cagggaatgg aaaaaggcgg aaaaattgcg attatcagcc
    34921 ttgctctggt ttgggtaggc tatggcgtga acgatatttt atttaaacag attgccaaat
    34981 caggctctgc tttccctgtc accctttcga ttgcctttat cggtgcaggc tgttttatgt
    35041 ttatctattt gctacttaaa cgcactcaat ggcacgttcc aagtgtctta accggcttat
    35101 tgctaggcgt gttgaacttc ggcaatattc tgttctatat tcaagctcac caagccatga
    35161 aagatgaccc aactttagtc ttcactggaa tgaacttagg ggtaatttgc ttaggcactt
    35221 tcgttggagc attcttcttt aaagagaaaa tccacaaagt gaactactta ggcgtggcga
    35281 ttgcgattgt ggcgattatt tgcttgtttt attggcggta aatatacttt ccaaaattaa
    35341 gggcgaacat aatgttcgcc cttaattttt gagtaatata gtgtaaaata ttcaataaat
    35401 tcgaccgctt gttgtaccga ttcatatttc ataaaggaca aaaaatgaag ctaaaagcct
    35461 tatctacact cattgcctct tcaatgctat atagtcagag ttttgctgta ccgccggaaa
    35521 aaacagctga agagccacag ccctttttca ataaaaccga atctgggtat gatttagtct
    35581 ttaatgatca aaactatcgt ttaattgaaa cggaattaaa tggcgagaaa atccaattcc
    35641 gtgcctttga gcggattgtg tatgtgaaca atccgattga gccagattac caaacaatta
    35701 atctgtatgt gccggaagct tactacaata atggcgaaat caatggctac caagcggata
    35761 ccgccccgat cttcttgcca aattcagtgg ggggctatat gccggcaaaa gcggcaagtt
    35821 atgataaaaa aagacgtgac ggttcaccag gtacgatttt ggtggcgtta tctaaaggct
    35881 atgtggtggc aagtgtggga gcaagaggac gcacgctaca aaaagaggga aaatacaccg
    35941 gcaaagcacc ttctgccatt attgattcaa aatccgccgc ccataaagcg tgtcggttcg
    36001 agtccgacca ctggcaccac tcactatgta aaatttagtt ttacatagtt tgctgtaagc
    36061 atttgaattt tgaatcgttt gtgtaggatt gttcaaatca tattcgcaaa tacacagtta
    36121 ctac
//
LOCUS       DANLXQ010000006        35319 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.006, whole genome shotgun sequence.
ACCESSION   DANLXQ010000006 DANLXQ010000000
VERSION     DANLXQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 35319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35319
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.006"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..465
                     /locus_tag="PXD94_000238"
     CDS             <1..465
                     /locus_tag="PXD94_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL6285147.1"
                     /translation="WLWMNSSLHKEWSCELLARNVIPAIENEQYMLLIDNGIPIAYCS
                     WADLNLETEVKYIKDINSLTPEEWQSGDRRWIIDWVAPFGHSQLLYKKMCQKYPDMIV
                     RSIRFYPKQKELGKIAYFKGGKLDKKTAKKRFDTYQEELATALKNEFNFIKK"
     gene            481..3342
                     /locus_tag="PXD94_000239"
     CDS             481..3342
                     /locus_tag="PXD94_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL6285148.1"
                     /translation="MGTRLTTLSNGLKNTLTATKSGLHKAGQSLTQAGSSLKTGAKKI
                     ILYIPQNYQYDTEQGNGLQDLVKAAEELGIEVQREERNNIATAQTSLGTIQTAIGLTE
                     RGIVLSAPQIDKLLQKTKAGQALGSAESIVQNANKAKTVLSGIQSILGSVLAGMDLDE
                     ALQNNSNQHALAKAGLELTNSLIENIANSVKTLDEFGEQISQFGSKLQNIKGLGTLGD
                     KLKNIGGLDKAGLGLDVISGLLSGATAALVLADKNASTAKKVGAGFELANQVVGNITK
                     AVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLAFAGIADKFNHAKSLESYAERF
                     KKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVIASPIALLVSG
                     ITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNHGKNYFENGYDARYLANLQDNMK
                     FLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGKHIKAD
                     KLVQLDSANGIIDVSNSGKAKTQHILFRTPLLTPGTEHRERVQTGKYEYITKLNINRV
                     DSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGT
                     TEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVETGKALHEVTSTHTALV
                     GNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDT
                     IDGNDGNDRLFGGKGDDILDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRKGDGNDIIT
                     DSDGNDKLSFSDSNLKDLTFEKVKHNLVITNSKKEKVTIQNWFREADFAKEVPNYKAT
                     KDEKIEEIIGQNGERITSKQVDDLIAKGNGKITQDELSKVVDNYELLKHSKNVTNSLD
                     KLISSVSAFTSSNDSRNVLVAPTSMLDQSLSSLQFARAA"
     gene            3417..5543
                     /locus_tag="PXD94_000240"
     CDS             3417..5543
                     /locus_tag="PXD94_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL6285149.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TAWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDEKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPEIKGDISFKNIRFRYKPDAPTILNNVNLEIRQGEVIGIVGRSGSG
                     KSTLTKLLQRFYIPENGQVLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIAL
                     SDPGMPMERVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNN
                     PKILIFDEATSALDYESEHIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEI
                     VEQGKHHELLQNSNGLYSYLHQLQLN"
     gene            5555..6991
                     /locus_tag="PXD94_000241"
     CDS             5555..6991
                     /locus_tag="PXD94_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL6285150.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
     gene            7232..8230
                     /locus_tag="PXD94_000242"
                     /pseudo
     CDS             7232..8230
                     /locus_tag="PXD94_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            8247..8990
                     /locus_tag="PXD94_000243"
     CDS             8247..8990
                     /locus_tag="PXD94_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6285151.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            9145..10203
                     /locus_tag="PXD94_000244"
     CDS             9145..10203
                     /locus_tag="PXD94_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL6285152.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAIPSTAKGLTRTKELEFIALQGTNTTSLVFENVELQDEWILADDAPEF
                     LAATRPAFLGLQFGLPFGLVERSLAEIEKSLPFRAILASEWQAQVDALNDIKTRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(10324..10866)
                     /locus_tag="PXD94_000245"
     CDS             complement(10324..10866)
                     /locus_tag="PXD94_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL6285153.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAEIPINPELQPFA"
     gene            complement(11018..12529)
                     /gene="glpK"
                     /locus_tag="PXD94_000246"
     CDS             complement(11018..12529)
                     /gene="glpK"
                     /locus_tag="PXD94_000246"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL6285154.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANVVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            complement(12533..13318)
                     /locus_tag="PXD94_000247"
     CDS             complement(12533..13318)
                     /locus_tag="PXD94_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL6285155.1"
                     /translation="MSKSLRNACIGEFIGTGFILFFGAGCVAAAQVAGANFGLWEISI
                     VWGLGVSMAIYISAGISGAHLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALI
                     YFMYSDLFTAAEAAQGITRGETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTIL
                     AIGDDKNGLPNKALAALLIGLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEI
                     ALTGGKEIPYFIIPIVAPICGALFGAWGYKNLIHNNLPANTQE"
     gene            13531..13890
                     /locus_tag="PXD94_000248"
     CDS             13531..13890
                     /locus_tag="PXD94_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL6285156.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
     gene            complement(13938..15260)
                     /locus_tag="PXD94_000249"
     CDS             complement(13938..15260)
                     /locus_tag="PXD94_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL6285157.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            15409..16233
                     /gene="dapF"
                     /locus_tag="PXD94_000250"
     CDS             15409..16233
                     /gene="dapF"
                     /locus_tag="PXD94_000250"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL6285158.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
     gene            complement(16404..16946)
                     /locus_tag="PXD94_000251"
     CDS             complement(16404..16946)
                     /locus_tag="PXD94_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL6285159.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            17070..17393
                     /locus_tag="PXD94_000252"
     CDS             17070..17393
                     /locus_tag="PXD94_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL6285160.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            complement(17456..18901)
                     /gene="xylB"
                     /locus_tag="PXD94_000253"
     CDS             complement(17456..18901)
                     /gene="xylB"
                     /locus_tag="PXD94_000253"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL6285161.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAMLELSATQDLQAVKAIGLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSVYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPTPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(19054..20373)
                     /gene="xylA"
                     /locus_tag="PXD94_000254"
     CDS             complement(19054..20373)
                     /gene="xylA"
                     /locus_tag="PXD94_000254"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL6285162.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWAAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
     gene            20615..21616
                     /gene="xylF"
                     /locus_tag="PXD94_000255"
     CDS             20615..21616
                     /gene="xylF"
                     /locus_tag="PXD94_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6285163.1"
                     /translation="MKLKSKLLAIATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSSKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
     gene            21683..23197
                     /gene="xylG"
                     /locus_tag="PXD94_000256"
     CDS             21683..23197
                     /gene="xylG"
                     /locus_tag="PXD94_000256"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL6285164.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            23201..24328
                     /locus_tag="PXD94_000257"
     CDS             23201..24328
                     /locus_tag="PXD94_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL6285165.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(24344..24874)
                     /locus_tag="PXD94_000258"
     CDS             complement(24344..24874)
                     /locus_tag="PXD94_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6285166.1"
                     /translation="MSENSLKTHYSAKELAELSLACLPKTKQGVLYQAKKQGWTTHKK
                     VGVGGEILEFALSNLPQEIQTEIRSRFMSAVVEAKPKKLPAVRAEVDVGNLTTKQREI
                     ADARMALVAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLVELVAVANAKKTAKRTIV
                     PIFSPIMPDFPLDSRD"
     gene            complement(24890..25153)
                     /locus_tag="PXD94_000259"
     CDS             complement(24890..25153)
                     /locus_tag="PXD94_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6285167.1"
                     /translation="MSTLEDIKKTAKDWHRADIIAALHKAGWSLRQLSLKHGYSQSST
                     LKNAVDRPWLKGERIIAEAIGISPEEIWPERYAQRNHKKFADR"
     gene            25337..25819
                     /locus_tag="PXD94_000260"
     CDS             25337..25819
                     /locus_tag="PXD94_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6285168.1"
                     /translation="MKIECKKVIGFGNRIEQSRKNAKLSRSEVCDALGGIAISTLQAW
                     EADTREPPISKLMALSQILNVEPCYLLTGERHSQSLNVTREPQPVYATKPQTEEGEVV
                     VIKAKLQEAIETLEEVLEITQREMKPAGKAQMVWAFYELLTQEKSEKEKMVGLLKLVA
                     "
     gene            25831..26082
                     /locus_tag="PXD94_000261"
     CDS             25831..26082
                     /locus_tag="PXD94_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285169.1"
                     /translation="MDKQAILKLFRDNSTAGNSQPSQEPIQVFNNSNVIIQNGDNATV
                     IAGCTVDNNTNLTCADKQRVHDLIHSLEQRNRASRDEGA"
     gene            complement(26372..28237)
                     /locus_tag="PXD94_000262"
     CDS             complement(26372..28237)
                     /locus_tag="PXD94_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL6285170.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(28377..28892)
                     /locus_tag="PXD94_000263"
     CDS             complement(28377..28892)
                     /locus_tag="PXD94_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL6285171.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            complement(28904..30043)
                     /locus_tag="PXD94_000264"
     CDS             complement(28904..30043)
                     /locus_tag="PXD94_000264"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL6285172.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
     gene            complement(30147..32006)
                     /locus_tag="PXD94_000265"
     CDS             complement(30147..32006)
                     /locus_tag="PXD94_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL6285173.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQAAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
     gene            32308..32858
                     /locus_tag="PXD94_000266"
                     /pseudo
     CDS             32308..32858
                     /locus_tag="PXD94_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(32902..33837)
                     /locus_tag="PXD94_000267"
     CDS             complement(32902..33837)
                     /locus_tag="PXD94_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL6285174.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(33848..34645)
                     /locus_tag="PXD94_000268"
     CDS             complement(33848..34645)
                     /locus_tag="PXD94_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL6285175.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            complement(34688..35308)
                     /locus_tag="PXD94_000269"
     CDS             complement(34688..35308)
                     /locus_tag="PXD94_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL6285176.1"
                     /translation="MDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQLTRGKRIDEL
                     SDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSNQIKLAVATS
                     SPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEKQECIVIEDS
                     VTGLIAAKAANLTTFLVNTDYQNEKFSIADKRMASLLDVIEKLKQH"
BASE COUNT        10441 a         7369 c         7402 g        10107 t
ORIGIN      
        1 tggctatgga tgaactcctc cctccacaaa gaatggagct gtgaactact agcacgcaat
       61 gtgattcctg caattgaaaa tgaacaatat atgctactta tagataacgg tattccgatc
      121 gcttattgta gttgggcaga tttaaacctt gagactgagg tgaaatatat taaggatatt
      181 aattcgttaa caccagaaga atggcagtct ggtgacagac gctggattat tgattgggta
      241 gcaccattcg gacattctca attactttat aaaaaaatgt gtcagaaata ccctgatatg
      301 atcgtcagat ctatacgctt ttatccaaag cagaaagaat taggcaaaat tgcctacttt
      361 aaaggaggta aattagataa aaaaacagca aaaaaacgtt ttgatacata tcaagaagag
      421 ctggcaacag cacttaaaaa tgaatttaat tttattaaaa aatagaagga gacatccctt
      481 atgggaacta gacttacaac cctatcaaat gggctaaaaa acactttaac ggcaaccaaa
      541 agtggcttac ataaagccgg tcaatcatta acccaagccg gcagttcttt aaaaactggg
      601 gcaaaaaaaa ttatcctcta tattccccaa aattaccaat atgatactga acaaggtaat
      661 ggtttacagg atttagtcaa agcggccgaa gagttgggga ttgaggtaca aagagaagaa
      721 cgcaataata ttgcaacagc tcaaaccagt ttaggcacga ttcaaaccgc tattggctta
      781 actgagcgtg gcattgtgtt atccgctcca caaattgata aattgctaca gaaaactaaa
      841 gcaggccaag cattaggttc tgccgaaagc attgtacaaa atgcaaataa agccaaaact
      901 gtattatctg gcattcaatc tattttaggc tcagtattgg ctggaatgga tttagatgag
      961 gccttacaga ataacagcaa ccaacatgct cttgctaaag ctggcttgga gctaacaaat
     1021 tcattaattg aaaatattgc taattcagta aaaacacttg acgaatttgg tgagcaaatt
     1081 agtcaatttg gttcaaaact acaaaatatc aaaggcttag ggactttagg agacaaactc
     1141 aaaaatatcg gtggacttga taaagctggc cttggtttag atgttatctc agggctatta
     1201 tcgggcgcaa cagctgcact tgtacttgca gataaaaatg cttcaacagc taaaaaagtg
     1261 ggtgcgggtt ttgaattggc aaaccaagtt gttggtaata ttaccaaagc cgtttcttct
     1321 tacattttag cccaacgtgt tgcagcaggt ttatcttcaa ctgggcctgt ggctgcttta
     1381 attgcttcta ctgtttctct tgcgattagc ccattagcat ttgccggtat tgccgataaa
     1441 tttaatcatg caaaaagttt agagagttat gccgaacgct ttaaaaaatt aggctatgac
     1501 ggagataatt tattagcaga atatcagcgg ggaacaggga ctattgatgc atcggttact
     1561 gcaattaata ccgcattggc cgctattgct ggtggtgtgt ctgctgctgc agccggctcg
     1621 gttattgctt caccgattgc cttattagta tctgggatta ccggtgtaat ttctacgatt
     1681 ctgcaatatt ctaaacaagc aatgtttgag cacgttgcaa ataaaattca taacaaaatt
     1741 gtagaatggg aaaaaaataa tcacggtaag aactactttg aaaatggtta cgatgcccgt
     1801 tatcttgcga atttacaaga taatatgaaa ttcttactga acttaaacaa agagttacag
     1861 gcagaacgtg tcatcgctat tactcagcag caatgggata acaacattgg tgatttagct
     1921 ggtattagcc gtttaggtga aaaagtcctt agtggtaaag cctatgtgga tgcgtttgaa
     1981 gaaggcaaac acattaaagc cgataaatta gtacagttgg attcggcaaa cggtattatt
     2041 gatgtgagta attcgggtaa agcgaaaact cagcatatct tattcagaac gccattattg
     2101 acgccgggaa cagagcatcg tgaacgcgta caaacaggta aatatgaata tattaccaag
     2161 ctcaatatta accgtgtaga tagctggaaa attacagatg gtgcagcaag ttctaccttt
     2221 gatttaacta acgttgttca gcgtattggt attgaattag acaatgctgg aaatgtaact
     2281 aaaaccaaag aaacaaaaat tattgccaaa cttggtgaag gtgatgacaa cgtatttgtt
     2341 ggttctggta cgacggaaat tgatggcggt gaaggttacg accgagttca ctatagccgt
     2401 ggaaactatg gtgctttaac tattgatgca accaaagaga ccgagcaagg tagttatacc
     2461 gtaaatcgtt tcgtagaaac cggtaaagca ctacacgaag tgacttcaac ccataccgca
     2521 ttagtgggca accgtgaaga aaaaatagaa tatcgtcata gcaataacca gcaccatgcc
     2581 ggttattaca ccaaagatac cttgaaagct gttgaagaaa ttatcggtac atcacataac
     2641 gatatcttta aaggtagtaa gttcaatgat gcctttaacg gtggtgatgg tgtcgatact
     2701 attgacggta acgacggcaa tgaccgctta tttggtggta aaggcgatga tattctcgat
     2761 ggtggaaatg gtgatgattt tatcgatggc ggtaaaggca acgacctatt acacggtggc
     2821 aagggcgatg atattttcgt tcaccgtaaa ggcgatggta atgatattat taccgattct
     2881 gacggcaatg ataaattatc attctctgat tcgaacttaa aagatttaac atttgaaaaa
     2941 gttaaacata atcttgtcat cacgaatagc aaaaaagaga aagtgaccat tcaaaactgg
     3001 ttccgagagg ctgattttgc taaagaagtg cctaattata aagcaactaa agatgagaaa
     3061 atcgaagaaa tcatcggtca aaatggcgag cggatcacct caaagcaagt tgatgatctt
     3121 atcgcaaaag gtaacggcaa aattacccaa gatgagctat caaaagttgt tgataactat
     3181 gaattgctca aacatagcaa aaatgtgaca aacagcttag ataagttaat ctcatctgta
     3241 agtgcattta cctcgtctaa tgattcgaga aatgtattag tggctccaac ttcaatgttg
     3301 gatcaaagtt tatcttctct tcaatttgct agagcagctt aatttttaat gattggcaac
     3361 tctatattgt ttcacacatt atagagttgc cgttttattt tataaaagga gacaatatgg
     3421 aagctaacca tcaaaggaat gatcttggtt tagttgccct cactatgttg gcacaatacc
     3481 ataatatttc gcttaatccg gaagaaataa aacataaatt tgatcttgac ggaaaagggc
     3541 tttctttaac tgcttggctt ttagctgcaa aatcgttagc gttgaaagcg aaacacatta
     3601 aaaaagagat ttcccgctta cacttggtga atttaccggc attagtttgg caagataacg
     3661 gtaaacattt tttattggta aaagtggata ccgataataa ccgctattta acttacaatt
     3721 tggaacaaga tgctccacaa attctgtcac aagacgaatt tgaagcctgc tatcaagggc
     3781 agttaatttt ggtcacgtcc agagcttccg tagtaggtca attagcaaag ttcgatttca
     3841 cctggtttat tccggcggtg atcaaatacc gaaaaatctt tctagaaacc ttgattgttt
     3901 cgatcttttt gcaaattttt gccctaatta caccgctatt cttccaagtt gttatggata
     3961 aagtactggt gcatcgaggt ttttcaacct tgaatatcat tacggttgcc ttagctattg
     4021 tgatcatctt tgaaattgta ctaagtggtt tgagaaccta tgttttttct catagcacta
     4081 gccgtattga tgttgaatta ggcgctaaat tatttcgaca tttattatca ctacccattt
     4141 cttattttga aaacagacga gttggagata cagtcgctag ggttagagaa ttagatcaaa
     4201 ttcgtaattt ccttaccgga caagcattaa cctcggtgtt agatctctta ttctctttta
     4261 tcttttttgc cgtaatgtgg tattacagcc caaaattaac cttggtaatt cttggttcat
     4321 tgccctgcta tattttatgg tcaattttta ttagtccgat tttaagacgg cgtttagatg
     4381 agaaatttgc ccgaagtgct gataaccaag cattcttagt tgagtcggta acagccatca
     4441 atatgattaa agcgatggcg gttgctccac aaatgacgga tacatgggat aaacagctgg
     4501 caagctatgt ttcatcaagt ttccgtgtca ccgtattagc aaccattggg caacaaggtg
     4561 tacaacttat tcaaaaaacc gttatggtga ttaacctttg gttaggggca cacttagtta
     4621 tttcaggcga tctgagtatt gggcaattaa ttgcctttaa tatgctatca gggcaagtga
     4681 ttgcaccggt gattcggctg gctcagctct ggcaagattt ccaacaagtt gggatttccg
     4741 tcactcgctt aggtgatgtt ttaaactctc caaccgaaca atatcaaggc aaattatcac
     4801 taccagaaat aaaaggcgat atctcattta aaaatatccg ctttagatat aaaccagatg
     4861 caccaactat tttaaataat gtgaatttag aaattaggca aggagaagtg attgggattg
     4921 ttggacgttc cggttcaggc aaaagtactc tgactaaatt actgcaacgt ttttatattc
     4981 ctgaaaatgg gcaggttttg attgatggac atgatctagc cttagctgat ccaaactggc
     5041 tacgccgtca aataggtgta gtgctgcaag ataatgtgtt attaaaccgc agtatccgag
     5101 aaaatattgc gctatcagat ccaggaatgc caatggagcg agtaatttat gcagcaaaat
     5161 tagcaggggc tcacgatttt atttcagaat tgcgtgaagg ttataacacc attgtgggtg
     5221 aacaaggagc ggggctttca ggcgggcaac gccaacggat tgcgattgct cgagctttgg
     5281 taaacaaccc gaaaatcctg atttttgatg aggcaaccag tgccctcgat tacgaatctg
     5341 agcatattat tatgcaaaat atgcaaaaaa tatgccaagg cagaaccgtg attttgattg
     5401 cacatcgttt atcgaccgtc aaaaatgcgg atcgaattat tgtgatggaa aagggggaaa
     5461 ttgttgagca aggcaagcac cacgaattac tgcaaaacag taacggactt tattcctact
     5521 tacaccaatt acaacttaat taagaaggaa aacaatgaaa atatggctta gtggtattta
     5581 tgaatttttc ctacgctata aaaacatttg ggcagaagta tggaaaattc gtaaagaatt
     5641 agaccaccca aacagaaaaa aagacgaaag tgaattttta ccggcacatt tagaactgat
     5701 tgaaaccccg gtttctaaaa aaccacgtct aattgcttat ttgattatgc tatttttagt
     5761 tgtggcaatt gtgcttgcca gtgtaagcaa agttgaaatt gtggcgactg ctcccggtaa
     5821 attaactttt agtggcagaa gtaaagaaat taaaccgatt gaaaacgcca ttgtacaaga
     5881 aattttcgtt aaagatgggc agtttgtgga aaaagggcaa ttattagtca gcttaactgc
     5941 attgggttct gatgcagata tcaaaaagac catggcttca ctttctttag ctaaactgga
     6001 gaactatcgc taccaaactt tgcttactgc cattgaaaaa gagtccttgc cggtgattga
     6061 tttatctaga accgaattta aagattcatc ggaagaagat cgactacgta ttaaacactt
     6121 aattgaggag caatacacca cttggcaaaa acaaaaaaca cagaaaactt tagcgtataa
     6181 gcgtaaagag gctgaaaaac aaacaatatt tgcctatgtc cgtaaatatg aaggtgcaac
     6241 acgtattgaa caagaaaaat taaaagactt taaggcactt tataaacaga agtctttatc
     6301 taagcacgaa cttcttgcgc aagaaaataa attaattgag gctcagaatg agctagctgt
     6361 ttatcgctca aaattaaatg aattagaaaa tgatctactc aatgtaaaag aagaacttga
     6421 attgatcacg caattcttta aaagcgatgt gttggaaaaa ttaaagcaac atattgaaaa
     6481 tgaacgccaa cttcggctcg agttagaaaa aaataatcaa cgcagacagg cctcgatgat
     6541 cagagcaccg gtttccggta cggttcagca actgaaaatt cacactatag gtggtgttgt
     6601 tacgactgct gaaaccttga tgatcattgt gccggaagac gatgtgttag aggccaccgc
     6661 tctggttcca aacaaagata tcggctttgt tgcagcaggg caggaggtga ttattaaagt
     6721 ggaaactttc ccttatacac gctatggtta tctaactggt cgaattaaac atattagccc
     6781 ggatgcgatt gaacaaccta atgtaggctt agtttttaat gcaactatag ctatagatag
     6841 gaagaatcta acatcgcctg atgggcgaaa aattgatttg agttcaggta tgacaataac
     6901 tgctgaaatc aaaaccggtg aacggagtgt aatgagttat ttactcagcc cattagaaga
     6961 atctgtcaca gaaagtttaa gggaacgcta atcgaaccaa aacaaagcca taaaagccat
     7021 tttgagcttt tatggcttta ttttttagtc cacaagcggt caaaaaagcc caatttttta
     7081 cacttttata acaaattgtt ctaactaaaa attactaatt cttttctttt ataagacgaa
     7141 tcctctattt tcatttcatt aacattgaac tagaaaaggg gatttatgag cctaagcatt
     7201 acgaatcttt ctcttggcta ccgcaaaaat cagcaaaggc ttatttgaaa agcacggtgt
     7261 gcaggtggaa aaaccggtga tgtttcgcag ctgggctcag ttggtggaag cctttttaag
     7321 tggcaatgtg aacgtggtgc atctgctttc gcctatgagt ttgtgggcga aatatggagc
     7381 aaatgctccg gtgaaagcgg taatgtggaa tcacttggca ggttcggctt taacggttcg
     7441 ccctgaaatc aacagtattg ccgaactctc cggcaaaacg gtagcaattc cgttttggta
     7501 ttcaattcat aatatcgttt tgcaacgatt attacgagaa aatggcttga aaatcaccga
     7561 aaaagagccg aaaagcggtg aagtgcgatt aagtgtaatg gctccgtcag atatggtggc
     7621 agcgttggca agtgaggcaa tttccggctt tattgtggca gagccgttca atgcgttagc
     7681 ggaagccaaa ggtgtgggga aggtgctgcg ttttagtggc gatgtgtgga aagatcacgc
     7741 ctgctgttta accctaatgc acgaatacga tattcaacac cgcccggaat gggtgcaaaa
     7801 agtggtgaat gtcttaaccg aagcccaagt gtccacgctt gccaaccgtg cagaaaccgc
     7861 tcaattattg gctcggggca gcggctacac gccgcacgat caaaaagtgc tggaaaaggt
     7921 actggctcca acccaagctc agtgggataa atatatcgaa acaggggcaa ttcaaaaccc
     7981 tcagtggcac caacagcgaa tcggtttcca accgtatccg ttcgattcct atatggaaga
     8041 gctggtcaaa atgctcaaac agacccacat tgccggcaat aatgcgtttt tacaacagct
     8101 tgacccggta gaggcagcga aagcgattaa cgagccgacc tttgtgcgaa aagcgattga
     8161 gcaaggcggt tgggttgaaa cctttaattt acaaaccggt tggacaagaa ccgagcaaat
     8221 tagtgtataa gcgataggaa cgaacgatga aacagaaata tatttacggc ttagccggtt
     8281 tattactgct acttttaggt tggcagcttg gcacagcctt gttaagccaa cggttgccga
     8341 tggcgtcaat gttagctccg gcaaaaacct gggaaagttt tacttggctg atttcctccg
     8401 gcgaaatttt tccgcactta tgggcgagtt tgcaacgcat tgctgtcggg ctactcgctg
     8461 cgttagtgat tggtgtaccg ctgggcttat tgctcggctt ctccaagcac cttgaaatgc
     8521 tttgcacgcc gtcattccaa ctactacgta tgatttcgcc actttcgtgg atgccgatta
     8581 tcgtgatgtt acttggtgta ggcgatttgc cgatttattt tttactggcg tttgccaccg
     8641 tttggtcgtt gattatttcc accacctcag gggtaaaaag cattgatcca caatggttac
     8701 aactgggtag agtaatgtcg gctacccgca aagaactgct gtgcaaaatt gttgtaccgg
     8761 ctattaccgg acacgttctc accggtttac gtgtggcggt tgggattgcg tgggtggtgc
     8821 tggtaccgtg cgaaatgcta ggcgtgaatc aaggactcgg ctattttatt ttagacaccc
     8881 gagaccgatt agcctattcc gaactgatgg cggtaattat aattatcggc ttaatcggct
     8941 gggcgttgga tagcttaatc cgcttggctt tagcgaaagg cggcagataa attgacaagc
     9001 ggtcgaaaaa ttgcattttt ttgcaaaaaa tcgaccgctt gtttattccc aaaaagcata
     9061 ttaccttaat ttattttatt tctagggcat aaaaaagcga tttaagatag cgacaaagta
     9121 aactaattat acgaggtaaa cacaatgtcg gttctttcat cttcttttat tcaatggctt
     9181 cagcagaacg ccgaaaattt agatcaatct aacgaacgag cggatttact cattccacaa
     9241 attgccgcac tagggctata taaaatcggc gtgccggaaa cgcttggcgg cagcggtggc
     9301 actttggttg atgcgatcaa agccgttacc gaactgggta atcactcgct ggctgcggca
     9361 tttgtttcgt ggggacatcg tacctttatc gagcatatta tcgcctcaaa aaatcctatt
     9421 ccccgccaaa gctatttggc ggatctgcta aatggcaatt taaccgcagg aacaggttta
     9481 tccaatgcga tgaaatttct ctccggcatt gaagagttga atgtgaatat tcgtgaagaa
     9541 aacggtaaac gctatttaaa tggccgcctg ccttgggtaa ccaatttacg ttcataccgc
     9601 tttgttgcta gtttcgttgc cggttttcaa gacgaacgca aaccgctagt gattgcaatt
     9661 ccctccaccg ccaaaggctt aacccgcacc aaagaattgg aatttatcgc attgcaaggc
     9721 acgaatacta cctctctcgt atttgagaat gtggaattac aagatgaatg gattttggct
     9781 gatgacgcgc ctgagttttt agcagccact cgccctgcgt tcttaggttt acaatttggt
     9841 ttgccctttg gcttggttga acgtagtttg gcggaaatcg aaaaatcgct ccctttccgt
     9901 gcaattctcg cctctgagtg gcaagctcaa gttgatgccc tcaacgatat taaaactcgt
     9961 ctgtttaatg gcttaaatga agaaggttat tttgtgcgta atccgaaaga gcttttccaa
    10021 gcgagaattg acattgtcaa tgtcgtcgcc caaactattc tgctagaact gcaagcgagt
    10081 ggcggacgtg gctatctgaa aaatgatact tcaggcttca gccgccgttg gaaagaagcg
    10141 gcatttctgc ccgttgttac gccaagtgcg gtacagctga gaatggtgct ggcgaatagt
    10201 taagcggata agagacaaat tgttgcctct tttattttgc aaaaaatagt ctaaatataa
    10261 ccgcttgcaa taaaaataaa aaaggatgca ttttttatgc ttcctcaatt ttacggctat
    10321 ctattatgca aaaggctgca attcagggtt aattggaatt tcggcaatgt tgttaacgta
    10381 gttacataat gttgccaagc tcacgcctaa aatcacatca atcgcatttt gttggttgta
    10441 acctgcggca aaaaattcgt ttaattgggc ttcggttaat ttggcttttt gtaacattac
    10501 tgcaagggtg aagcgagcca gagtgtctaa tttgttatcg tctatgcgag ccgtggcacg
    10561 taaagcattt accacttctt caggcatttt taatgctttt aaggcgattt tagtatgacc
    10621 tgccacgcag aaaccacagc cgttgaccac tgctgcggtg atttgtacca cttcacgttc
    10681 ttcagcggtt aagctgttac gactattaat accacccaca gtgcggtaag tttccagtgc
    10741 tgttggtgcg ttggctaata cgccgatcag atttgggata aaaccgtttg cttgttgcac
    10801 tgcttttagg gcttcttgtg ccgtttcagg agcggattca atagtgtgaa tttgaaattg
    10861 agacataaat gttccttatc aggttgttta ttggtgtggc tcatcataaa gaaaaggcta
    10921 atttagaaca aatcaaaatc actatttaat ataactaaaa gatcttaaag ggagtatagt
    10981 tgcctatact ccctttgttt atgcacaggt tgcgttttta ctcttctgcg tcttcttttg
    11041 cccattctaa tgagcggcga acggcttttt tccaaccttt atagcgtttc gcccgttttt
    11101 cttggtcgcc gtccggtgtg aaggtgcgtt caatttccgc tttgtctttc agttcttcca
    11161 aatctttcca gaagccgaca gcaatacccg caagataagc tgcccctaaa gcagtgactt
    11221 ctttcacttt cggacgctca actttggtgt ctaaaatatc ggcttggaac tgcattaaga
    11281 agttgtttgc ggttgcacca ccgtccacac gtaacgcttg tagtttcgct ttggagtcag
    11341 actgcattgc ttctaacaca tctcgggttt ggtaagcgat agactctagg gtcgcacgca
    11401 caatgtggtt acggtttgcc ccacgggtta aaccgaaaat tgagcctcgt gcatacggat
    11461 cccaatatgg agcacctaag ccggtaaagg caggtacaac ataaacaccg ttagagtcat
    11521 cttctttagt ggcaaaatat tcgctgtcaa agctatcgtg aacaattttc agctcatcac
    11581 gcagccattg aatagacgca ccggcaataa atactgaacc ttccaacgca tattccggct
    11641 cgccttttgc gttacacgca atggtcgtta ataaaccgtt ttcagattga atcgcagtgt
    11701 taccggtgtg catcaacata aaacagcctg taccataggt attcttcgct tgtccttctt
    11761 tcacgcataa atgaccgtag agagcggctt gttggtcacc tgcaataccc gcgaccggaa
    11821 tacgcacacc gccttgaccg ccgatattag tttgcccgta aacttctgat gaatttttca
    11881 cttcaggtaa cattgagcgt ggaatattga gcaattccag cattttatcg tcccattgtt
    11941 tggtgtgaat gttaaacaac atagtacgag aagcgttggt gtaatcggta acgtgtaccc
    12001 gcccttgggt taatttccac accagccaag tatcgaccgt accgaacagc aattcgccac
    12061 gttctgcttt ttctcttgcc ccttctacgt tatctaaaat ccacttcact ttagtgccgg
    12121 agaagtacgg atcgactacc aagcctgtcg ttttacgaat atattcctcg tgtccgtctg
    12181 cttttaattt atcggtaata tccgatgtgc gacgacattg ccacacaatc gcattataaa
    12241 caggtttacc ggtctctttt tcccacacaa tcgtggtttc acgttggttt gtgatcccga
    12301 ttgcggcaat gctatccggc ttaataccgg cttttgccac cacttcattt aaggtggaac
    12361 tttggcttga ccaaatttcc atcgggttat gttctaccca gcctgcttgc ggataaatct
    12421 gggtaaattc acgctgagcc acttcaacca cattggcatt tttatctaat aaaaccgcac
    12481 gagagctggt tgtaccttgg tcgagagcga tgatatattg tttttccata gattactcct
    12541 gagtatttgc tggaagattg ttatgaatga ggtttttata accccaagca ccgaataatg
    12601 caccgcagat tggggcgaca atcggaatga tgaaatacgg aatttctttt ccgcctgtta
    12661 aagcaatttc accccaaccg gcaagataag caaagagttt cggaccaaaa tcacgagccg
    12721 ggttcatcgc aaagccggtt aacgggccgg ttgctccacc aattaccgca atcaataaac
    12781 cgatgagtaa tgcggctaag gctttattcg gcaagccgtt tttatcatcg ccaatcgcta
    12841 aaatagtgga catcaacacc acagtaatca caaattcaac gatgaaggcg gtaaccaatg
    12901 taatattagg gtttggatag gtggagaaca caccggcaaa gccaacagtt tcgccccgag
    12961 taatgccttg agcggcttcg gcggcagtaa agagatcgct atacataaaa taaatcagtg
    13021 cgacagagca aaacgctccg gcaacttggg cgatgacata gggcaaaatt ttatgctttt
    13081 caaagccgta gaatgcggca agtgctatag tgaccgcagg gttaaggtga gcaccggaaa
    13141 taccggcaga aatgtagatt gccatggaaa caccaagccc ccaaacgatg gaaatttccc
    13201 ataaaccgaa attcgcaccg gctacctgag cagcagcaac acaaccagca ccaaagaaaa
    13261 gaataaaacc tgtaccgata aattcgccaa tacaggcatt acgtaaggat ttgctcataa
    13321 agtttctcct taagatgaaa atgattggaa agtgatgaaa aaatgtttga atatgaacat
    13381 atttcggagt gatattgcca aagcggcatt ttttaagcaa ataggaagtt tcaaatttgt
    13441 gaaatccatc acattatttt tctaaatcac tcatttttag gtatgatgtg agaaaacgtt
    13501 agatgaggaa aaatgatgtc ttcaatactt atgatttcaa tcggggcggt acttggagct
    13561 atgtgccgct ggcaattagc cgtttggcta aacccgatac tcagccaatt cgcctttggt
    13621 actttagcag caaattggct tggttgtttg ttaattggta ttgcaatggg gtttaattta
    13681 ggcgagagcc agcggttgct gttcatcagc ggttttttag gcagttttac caccttttcc
    13741 agtttctctt tagaactaac ggaaaaacta ttagcagaaa aatgggggca gtttgtgaca
    13801 gtgctgagtt tacatttaat cggtgggctt ttgcttactt tgataggtgt aatgcttgca
    13861 agagccgtta caagcggtcg tttttgctga aaattttgca aataaaaaac ctcccaaacg
    13921 ggaggttttg cgagcgatta aagtaggata ttggaaagta ataaaccaaa tccgatactg
    13981 aaaatcatac taagtaagcc cggtaacata aagctatggt tgaaaatata tttaccgata
    14041 cgggttgtgc cggtggtgtc gaagtcaatc gaggcaataa tcgggccgta gttcggaatg
    14101 aagaagtagc cgtttaccgc cacaaatacc ccgattagca ccggtgcagg aatgccgata
    14161 gcaatcgcta cagggaataa ggttgcaacc gttgcccctt ggctgtttac caatacggaa
    14221 agtacgaata atgcgaaagc aaatgcccaa ggtgcggttt caactaagcc tgaaaccatt
    14281 gatttcactt cgtccatatg accttgcatt aaggtatcgc ctaaccaagc aataccgaaa
    14341 atcgcaatca ctgcacgcat accggcgtgg aagacagaac ctttggtgat ttcattaccg
    14401 tccggtttgc agaagaagat aatcaacgca ccaatactta acatcacaat ttcaatcgtg
    14461 tgagccatcc ccatcggctt accatcaaac acagggcgaa gagctggaat tgcgcccatt
    14521 aataccacta ataatgcacc gaataggaat aagcctactg ataatttcgc agtcggtttt
    14581 aacggtaatt cggttggatt ggtagtaatg tgattttctt tcacatattc agggtcttgc
    14641 aataagcgtt gataatttgg gtcgtctttt aattctttgc ccattttatt tacaaacaca
    14701 caagctaaac ctaagcccaa taaggtcgca ggaatagtca cacttaacac gttacccaat
    14761 gtaacacctt gaggttcaag gaaggcaacc acagcaacta ctgccgcagc aatcgggctg
    14821 gcgacaatcg caaattgtga ggcgattact gccattgaaa gtggacgctc agggcggata
    14881 ccgttgtggc ggcttacctc tgcaattaca ggtaatacag aataagcaac gtgacctgta
    14941 ccggcaagca cggtgaatgt ccaagttaca agcggtgcga taaaggtaat gtatttcggg
    15001 tttttacgca aaatgcgagt ggcaattttg atcatatagt ccaaaccacc ggctgcttgc
    15061 atggctgctg cagccgacac aaccgccata atcataaaca ttacatcaat cggtaaaccg
    15121 gcaggtttta gcccaaaccc aaatgaaaga atggctaagc ccaagccacc gaacacccct
    15181 aatccgatac cgcctacacg ggcgccaacc aaaatgcata gtaacactat ggcaaattgg
    15241 ataataaaca tcgcagacat aattttttgc tcctaaataa gtaaatgaaa ctctgtgttt
    15301 gaaaaaatct gcatttttca tcaaattaat cggcacgtac tatatcaata aataaaacta
    15361 atctctataa tctaaatcaa taaaatttct aattaattaa ggaataatat gcaattttca
    15421 aaaatgcacg gcttaggcaa tgattttatg gtggttgatg gtgtaaccca aaacgtttac
    15481 cttaccgaag agatgatccg tacgctttct gatcgccatc gtggtatcgg tttcgaccaa
    15541 cttttattgg ttgaaccgcc ttacgaccct gaattagatt ttcactatcg catttttaat
    15601 gcagacggca gtgaagtgtc gcaatgtggc aatggggcga gatgttttgc ccgttttgtt
    15661 acgctcaaag gcttaaccaa caaaaaagac attcacgtta gcaccgcaaa aggcaaaatg
    15721 gtgctaagtt tgcaagaaga cggcaaagtg cgtgtgaata tgggcgaacc gatttgggaa
    15781 ccttcgcaag tgccttttac agcaaataaa tttgagaaaa actatatttt acgcaccgag
    15841 ctacaaaccg tgttatgtgg cgtggtttca atggggaatc cgcactgcgt actgcaagtt
    15901 gatgatgtga aaaccgcccc ggtaaatgaa ctcgggccgt tactagaaag ccacgagcgt
    15961 tttcctgaac gggcgaatat cagctttatg caagtagtga atcgcaatca cgttaaatta
    16021 cgggtgtatg agcgtggagc aggcgaaacc caagcctgtg gtagcggtgc gtgcggcaca
    16081 gtggcggtcg gcattatgca aggcttgtta gataacaaag tgcaggtgga tttacccggt
    16141 ggaacgctga ttattgaatg gcaaggagta gggcatcctc tgtatatgac cggcgatgcc
    16201 acccacattt atgatggatt tattaaactg taagttttcc catagggagg caagcggttt
    16261 atttttaccg tttttttgca aaatagggtt ggtttataca aaagtaccag cgtggacgct
    16321 ggcactatca gactgatgta taacttttgt ttaaattcaa cttatagatg gtgcaagcgt
    16381 cctcgcttgt accaaaacag gtttcactcg agtaacttct tcaccaactc caacaccaca
    16441 ggtaattcgg taacaccttc tctatccaaa aaatgcccgc cttgatttaa ccgaatataa
    16501 tccgcgtgca aatgttgggc taacgcatca ctgtattggt gattcacgac cttatcatct
    16561 aacgcactta ccacatagct tggaaagctc gatacgctcg gcaaaatcgc ataatagttg
    16621 gtaaaggcag acagttcggg taagttttct accggttgat agaaccccga cacaaacacc
    16681 gcccctttaa tcttggcgta atttttggct aaaaaattca aaatggcaat gcaacctaaa
    16741 ctatgcccga caaaaaccgt ttgatcggtc agctccacat tttgtgcaag atgatttaac
    16801 caaccatcta atgacggctt tgccgaatcc ggcatattca accgctcaca actgacacca
    16861 cacttcgcta attcttgctc cagccaagga aaccaatgct tatcggcatt cgccgtataa
    16921 ccgtgtacga tataaacctg tgccatttca cacctcctga ttcaccaaag attgtaccat
    16981 agcttaatca ggaaaaaaca agcggtgatt tttttaaatt tttttgcaac tcaggtaaaa
    17041 tagcacgcat ttacgcaaag aggtcatcaa tgaacgtttg gattttactt gcgatttcca
    17101 tctgcctcga aatcgctgcc accaatttac tcaaattaag caacggcttc accaaagcta
    17161 taccgactat tggctcgctc gccttatacg gtttatcctt ttactttctc tctattattt
    17221 tccgcaccct accggtcgga atcgtgtatg ctatttggtc cggcgtaggg attgtcctta
    17281 ccgcccttgt tgcttatttt gctttcggac agaaaattga cttggcaggc ttaatcggca
    17341 tcagcctcat tattgccggt gtattggtga ttaatttgtt ttctaaagtg tgatacaagc
    17401 ggttgatttt acaaaaaaat ctgcaaaatc aaccgcttgt aatagttggc aagaattata
    17461 acccctttaa ccgccggtaa agctcagtaa atttttggcg tttttgttga tattctgcat
    17521 agcgggcagg ggttggttgg taaacctgtt ccaggggtaa cggctgacag aacgtagcaa
    17581 tatcctgatt tgggtttagc gcaatttgag cgagttttgc cgcccctaat gccggaccga
    17641 catcaccact ggtgcggtat tcaaaggttt taccgctaat atcggcaagt agttgtcgcc
    17701 agtacacact ttttgctcca ccgccaatta atgcaatatt gtcagcaact acaccagttt
    17761 cgtggagtac ctcaatccct tcagccaaag cgaaacttac cccttcaatc acggcttttg
    17821 ccatcgttat ttggttgtca ttatggctta atccccaaaa tacgcctttc gcataagggt
    17881 cattatgcgg tgtacgttca cccgataaat aaggtaagaa aatagcatca gatgccgttt
    17941 ggcttgcttc aacctgttgg aataaagtcg atacatcttt tagtcccaat gattttactg
    18001 cccaatctac cgcagaagcg gcacttaata ttactgacat cagatgccaa cgatcaggta
    18061 aagcgtggca aaaactatgc actgcttttt gcggattagc ctgaaattta tcgctcacta
    18121 caaaataaac ccctgaagtc cccaatgaga gcatagcttg cccggtttgg tataagccaa
    18181 tgccgattgc accggcagca ttatcgccac cgcctgccac aatcggcaca gatttcattt
    18241 tccattgctc ggcaagggtt ggacgtaaat aacctgtgat ttgattgcct tcaaacagtt
    18301 tcggcatatt ttcaatgtct aatccgcaag catttagtag cgatttgttc caattccgtt
    18361 tacccacatc aagccacatt gtgccggagg catcagacat atcagaggca tattcgccgc
    18421 tcatcaacaa gcggagataa tctttcggta gcaagacttt gctgactttt tcggcaatat
    18481 cgggctggtg tttatcaacc caacgcaatt tcggagcggt aaagcccggc atcatcaggt
    18541 tgcctgtaat ttcacggcta tttggcacaa gctgttctaa ctcttcgcat tccataaaac
    18601 tacgtccgtc attccataaa atcgcagggg aaagtacatt atctgcgtta tctagtaaag
    18661 tcgctccgtg catttgcccg gttaaaccaa tcgcttttac tgcttgtaaa tcttgagtag
    18721 cggaaagttc taacatcgct tcattggtgg cattccacca atcttgcgga ttttgttcag
    18781 accacaatgg gtgtggacga aaaataggaa gtgatttttg tgtgatggca ataatgtgtt
    18841 gattttcatc gagtaacacg actttcacac ccgatgtgcc gagatcgatt ccaatgtaca
    18901 taaatattcc tttgttattg aatacaagcg gtcattttat tcggaacatt tgcaatttta
    18961 agttttggag atctatcacc cctctttagc aaagaggggg atcagatcga gcttaacttt
    19021 gcaaaaaatt gataaaaaac aaccgcttgt agattaacgg taaatatagc tatttaccaa
    19081 attttccaaa tattcttgct gaccggaaac aggttttgga tctaaccctt gggtttccac
    19141 tatttttgcc aaatcttcaa ggcttgcttt accgtttaga atttgtaagc ccaaatcttg
    19201 gttccagcca gcgtagcggt tatctactac tttctgtaat gcttgatctt ccagcatttt
    19261 tgccgcacgt tttagcgaaa gggctaacac atcaattgcc ccgatatgtg cgtggaataa
    19321 atcatacgga tcggtacttt ggcggcggat tttcgcatca aagttgaaac cgcccgttgt
    19381 gaaaccacct gcttttaaga tttcatacat cacaagggtg ttttcttcca cgctgtttgg
    19441 gaattggtcg gtatcccaac ctaattgcgg atcacctcgg ttggcatcaa tcgagccaaa
    19501 aatatctaat gcggtggcag tcgcaatttc gtgttggaaa gtatgccctg caagagtggc
    19561 gtggtttgct tcaatgttga ctttgatctc tttttctaga ccaaactgtt ttaagaaacc
    19621 gtaaacggtg gcaacatcgt agtcatattg atgtttggtt ggttcttgtg gttttggctc
    19681 aattaataaa gtgccgttaa aaccgatttt gtgtttatgt tccaccacta actgcataaa
    19741 gcgaccgatt tgttcacgct cacgttttaa gtcagtgttg agtagggttt cataaccttc
    19801 acgtccgccc cataatacat aattttcgcc gcctaagcgt ttggttgcgt tcattgcggt
    19861 aaagacttgt gctgccgccc aagcaaagac ttccggattc ggattggttg aagcacccga
    19921 catatagcga ggattggtaa agcagttcgc cgtaccccat aataatttca cgcctgtttc
    19981 agcttgtttt tgctctaaaa tatccacaat agtgttgaaa ttgtgctgat attctttgaa
    20041 attgttgcct tccggtgcaa catccacatc gtggaagcag tagtaaggaa tgcctaattt
    20101 ttggaaaaat tcaaaggcaa tttccgcttt ttgcttcgca ccttgcagta aatcccccgt
    20161 tttttgccag cttctgtcaa gcgaacccac gccaaacata tcgttacccg tccagcagaa
    20221 ggtgtgccaa tagcaaaccg ctaaccgcag atgttctgcc atcgttttgc ctaaaaccac
    20281 ttcatccggg ttgtaatgtt tgaatgcaaa cgggttggtg gaattagccc cttcatattg
    20341 tacttttgag attttgtcga aatagttaga cataataatg ctcctatgaa ttggaaaaat
    20401 aatctttcgg taacatcatc ggaaaattat gcaaaacctt caattacgat atttcgtttt
    20461 tggatttcga ttattggtta atgtggcgta gatcacaaaa gtgaaaaatc ataatgatat
    20521 taataaatat gataattgaa cttcatttga aataagccca gactgcttac gggcgaaaaa
    20581 gatgctcaca ttttcataac caatgaggtg aactatgaaa cttaagtcta aattattagc
    20641 cattgctacg gcgactttaa tggtactcag caatgcttct gttgctaaag atctaaaaat
    20701 cggtatgtca attgatgatt tacgcttaga gcgttggcaa aaagaccgag atatttttgt
    20761 gaataaagcg gaaagcttag gagcaaaagt cttcgttcaa tcggcaaatg gcgatgcaac
    20821 cgcacaaatc tcacaaattg agaatatgtt gaataaagat attgatgtat tggtgattat
    20881 tccgtttaac ggcgaagtgt tatccaatgt gattgccgag gcgaagaaag aaggtgttaa
    20941 ggtacttgct tacgaccgct taatcaataa tgccgatgtg gatttttatg tttcatttga
    21001 taatgaaaaa gtaggtgaat tgcaggctaa aagcattatt gggcaaaagc cggaggggaa
    21061 ttatttccta atgggtggtt cacctgtaga taataacgcg aagctgttcc gtaaaggtca
    21121 aatgaaggtg ctacaaccgt taattgacag tggaaaaatt aaagtggtag gcgatcaatg
    21181 ggtagattct tggcttgctg aaaaagcatt acagattatg gaaaatgcac taactgctaa
    21241 taaaaataat attgatgcag ttgtagcttc aaacgatgca accgcaggcg gtgcaattca
    21301 agcgttaagt gcccaagggt tatccagcaa ggttgcaatt tccggacaag atgcagattt
    21361 agccgcaatt aaacgtatta ttgccggtac gcaaacgatg acagtgtata aaccaattac
    21421 caatttagcc gacaaagctg ccgaaattgc tgttgcatta ggtaaagatg agaaagtaga
    21481 aatgaatgct caattaaata acggtatgaa agaagtaccg gcatatttat tagagccggt
    21541 tgttgtaact aaagaaaata ttgatgacac agtaattaaa gacggcttcc ataccaaaga
    21601 tgcagtatat aaataaatca ctttaatttt tctagatggc gaggcttcct cgccattttt
    21661 gaaaataagg ggctaactaa gtatggcgaa attattagaa atgaagaaca tcactaaaaa
    21721 attcggtgag gttgtggcat taaataatat ttcaatttcc cttgaaaccg gcgaaatact
    21781 ttctttatgt ggtgaaaatg gttcaggcaa atcaactctg atgaaagtat tatgtggaat
    21841 ttatcctgcc ggtgattatg aaggggaaat ctatttttcc ggcgaaaaat tgattgccag
    21901 aaatatcaaa gatactgaag aaaaaggcat ttcgattatt caccaagaat tgaccttagt
    21961 aaaaaatatg tcgattttgg agaatatgtt tttagggaat gaaattacca aatcagggat
    22021 tacagatgac aatgaaatgt atctgcgttg taaaacctta cttgaacaag tgcagttgga
    22081 tattgaccca aacaccaaag ttggagagct aggtttaggg caacaacagt tggttgaaat
    22141 tgccaaagca ttgaataaac aggtacggtt gttgatttta gacgagccta ccgcctcgtt
    22201 aaccgaaaaa gaaaccgaga ttctgctgag tttaattaaa gatttacaag ctcacaatat
    22261 tgcttgtgtt tatatttcac acaaattgaa tgaagtgaaa gccatttccg acaaaatctg
    22321 tgtcattcga gacggcgaac atatcggtac tcgcagtgca gagggaatga gtgaagacga
    22381 tattattacg atgatggttg gacgtgagat tacctcgctc tacccacacg agccacacaa
    22441 tattggtgaa gaagtccttc gagttgaaaa tttaaccgct tggcacccaa ccaatacaca
    22501 tatcaaacgg gttgataatg ctaattttgt actacgccaa ggtgaaattt taggtattgc
    22561 tggtttggtc ggttcagggc gaaccgagat ggcacaatgt atttttggct cttaccaagg
    22621 caaatatcaa ggtgatattt ttctgcataa gcaaccgatt aaaatcaaaa attgtgcaca
    22681 agcgattgaa aaccatattg tgatggtgcc ggaagatcgc aaaaaacacg gcatcattcc
    22741 aattatgggg gtgggtaaaa acataactct ctcttcatta ccgcaatttt gttttggcaa
    22801 aaaagtaata aatgaaccgc tggaagaaac cattatcaac caatctattg caaaactgaa
    22861 agtgaaaact tcctcgcctg aattagcaat tggacggtta agtggcggta atcagcaaaa
    22921 agcaattttg gcaaaatgcc tattgcttaa ccctaaaatc ctgattttag atgagccgac
    22981 ccgtggtatc gacgtgggag caaaatatga aatttataag ctcatcaacc aattagctaa
    23041 agaaggtatg gcaattatcg tcatttcctc cgaattacct gaagttttag gcattagtga
    23101 tcgtgtattg gttatgcacc aaggcaaaat taaagcagat ttgattaacc aagaccttac
    23161 ccaagaaaaa gtaatggaag cggcactcaa ggagtaacct atgaataagt taaaatcgat
    23221 taatttacaa gtttatatta tgctgattgc aattgttgtg attatgagct ttttctctat
    23281 tgcaactgac ggtgcttatt taagtgccag aaatatctcc aacctgttac gccaaacctc
    23341 cattaccggt attcttgcca tcggtatggt atttgtgatt atttccgcag aaattgattt
    23401 atcggttggc tcgctaatgg gcttactcgg tggatttgcc gccatcagta atgtatggtg
    23461 gggctggccg ctctcgttaa ctattatagt tactttaatt ttaggcgtca tgctgggtgc
    23521 ttggagcggt tggtgggttg cttatcaaaa agtgccgtcc tttattgtaa ctcttgccgg
    23581 tatgctggcg tttcgtggtg tattggtcgg cttaaccaac gggacaaccg tttcaccgat
    23641 tagcaatgaa atgacaacca ttggtcaagg ctatttgcct gatatggtgg gtatgatttt
    23701 aggtactatc gggatatttt gctttattgt ttggggtagc taccaacgca aagccagaca
    23761 aaaactcaat cttactgtgc cttctatgtc gaaagacacg ctgaaatacg gactagttgc
    23821 aattatcgta ttaggtgcga tctacctact caatgattac cgtggtgtgc cattcccggt
    23881 attggtgttg gtgattctag ccattgccgg cacattcttg gccagaaaaa ccgccttcgg
    23941 gcgtcacatt tatgcgattg gtggcaacat tgatgccgct cgcctctcgg ggatttcagt
    24001 tgaaaaagtc aaacttgcga tttttgcctt aaacggctta ttagttgcca ttgcaggctt
    24061 gattttaagc tcacgtcttg gagccggcgc accttccgcc gggcaaaatg ccgaattaga
    24121 tgcgattgcc gcctgcgtga ttggtggagc cagtttagcc ggcggtatcg gtagcattta
    24181 tggcgtagtt attggagcat ttattattgc tttgctggat aacggtatga gtatgcttga
    24241 tgtgccaacc ttctggcaat atatcgttaa aggtggaatt ctactgcttg cagtttgggt
    24301 agataccatc agcaagaaaa aagcctaatt ggataacaag cggtcaatct cgactatcca
    24361 acgggaaatc cggcataatc gggctaaaaa tgggaactat tgtgcgtttt gccgtctttt
    24421 tagcatttgc tacggcaacc agctccacca agtgcggtgg catttcgcct tgtttggcaa
    24481 ggttgcaaag gtaggttatc gctttaatcc ggctcattga gccttccagt tccaacacat
    24541 acgccaccag tgccattcgt gcatcagcaa tttctcgctg tttggtggtc aggtttccca
    24601 catccacctc cgcacgcact gccggtaact ttttcggctt ggcttccacc accgccgaca
    24661 taaagcggga gcggatttcg gtttggattt cttgagggag gtttgagagt gcaaattcta
    24721 aaatttctcc cccaactcca acctttttat gagtagtcca tccttgtttc tttgcctgat
    24781 acaaaactcc ttgctttgtt ttaggtaaac aggctaaact taactcagcc aattcttttg
    24841 ccgaatagtg tgtttttaga ctgttttcac tcataaaaac ctctttaagc tatctgtcgg
    24901 caaatttttt atggtttcgt tgagcataac gttcaggcca aatttcttcg ggtgatatac
    24961 cgattgcttc ggcaataata cgctcaccct ttaaccacgg acgatctaca gcgtttttca
    25021 aggtgctgga ttgactgtag ccatgcttca atgagagttg tctaagcgac caacccgctt
    25081 tatgtaaagc agcgatgatg tcggccctat gccagtcttt cgctgttttt ttaatatctt
    25141 ccaatgtact cattagataa ctcctttcgc ttaacgattt agataatata cctattttta
    25201 ctaaaaataa agtgtaaaaa ggttgcttta tagttgattt atgagcaaat aaacttagtt
    25261 tgctgtaagt tattgattta tttataggtt tttagagtgt aaaaggctaa aaatctcaac
    25321 ttattttttt acagttatga aaatagagtg taaaaaggtt attggtttcg gtaaccgaat
    25381 agaacagagc agaaaaaatg caaaattaag tagatctgag gtttgtgatg ccctaggagg
    25441 tattgcaata tctacattac aagcgtggga ggctgataca agagaacctc ctatatcaaa
    25501 acttatggct ttgtcacaaa tacttaacgt tgagccttgc tacctattaa caggtgagcg
    25561 acactcgcaa agccttaatg tcacaagaga gccacagcct gtgtatgcca caaaaccgca
    25621 gacagaagaa ggggaagtag ttgttatcaa agctaagtta caagaggcaa tagaaacgtt
    25681 ggaagaggtg ctggaaatta cccaacggga aatgaagcct gccggaaaag cccaaatggt
    25741 gtgggctttc tacgagcttc tcacgcaaga aaaaagcgaa aaagaaaaaa tggtgggatt
    25801 actcaaactg gtggcataac aggggacaat atggacaaac aagccattct caaattattc
    25861 agagacaata gcactgccgg caacagccag ccaagccaag agcctataca ggtgtttaat
    25921 aacagcaacg tcatcattca aaacggcgat aacgccacgg tgattgccgg ctgcacggtg
    25981 gataataaca ccaacctaac ctgtgccgat aaacaacgtg tacacgacct cattcacagc
    26041 ttagagcaac gcaaccgagc cagccgagat gagggcgctt aaaattgttt taaaatccgt
    26101 ttaaacctgc tcagacctat gcaaaaaata acgtaaaaat agcccatttt tttctatttt
    26161 tgctcatttt cgttttttgc ataactcacc ccaaacaaaa acggcgaagc cttgtatttc
    26221 ctagcctcgc cgttattttt ccgcccaaat ttttttcttt ctctctatgc aaaaaagatc
    26281 agtaccccac actatgttgc cagacatact caacaatttt accgtcaacg tcagattacc
    26341 aaaaatgacc gcttgtaatt tagaggagtg tttagagcgg aaataacatc ggaatcacaa
    26401 acaccgacac cagcatcacc agtacagtaa acggcacacc aactttaacg aaatcaataa
    26461 atttatagcc tgccggtgct aataccatcg tatttaccgg tgaagaaacc ggcgtcataa
    26521 aagcggcgga ggctgaaaca gcgaccgtca tcacaaaagg tgccggtgag tagccaagtt
    26581 ggttagcaat cgcaatggct atcggcataa ccaaaattgc agtcgctgtg ttggaaataa
    26641 aagcactgat aatcgctgtt actacaaata atactattag aacaaaatgt acatctaagc
    26701 cttgcattgc atcaagtaga agcctaacgg ttaaatccac accaccggtt ttttgtaagg
    26761 caatagaaaa cggcatcatc cccacaatca acacaatgct tggcatatga atcgaatcat
    26821 aagcactttt catatccaca cagcggaact tgcctaacat taagcaggca atcaacgctg
    26881 ccatcacgtt tggcacaatg ccggtaatca tcagcaacac catagtcagc actgagaaca
    26941 gagcgtgagg ggcttggctt aacgccggag gggcgttttt actttctgcc ggatctctaa
    27001 gcaagaagaa atctcgatga tcgtcgtcca tacggtgaat ttgttgccat acgcccatca
    27061 ccaataaaat atcgccgaat ttgagtggcg tatcgagcag ttcctcttgt atgatttcat
    27121 caccacgttt taagccgaca atgcttaaat ggtatcttga gcggaatttg agttcccgaa
    27181 tagttttacc gatacattct gcttcaggca taaccgaaat ttccgccata cccaccgagc
    27241 gatattgtga ggaaaagtat tcgcctttta gctcaatcgg gcgaagtttg agctgttcac
    27301 agcgttgttg gtaagtttca tcatcgaggg cagtatcaag cagtaaaata tctttcggct
    27361 ggaaaatttc gtttaccgag gcactgacgg taatttttct aaaccgctta gtgcgttgaa
    27421 ttgcaatgat gtttagccct tgctcaatcc gtaaattcag ttgcccgata gatttgccga
    27481 cacagtctga atctgcttcc accagcacca ttttggctcg ctctttaagt tgatactctt
    27541 caattaactg cgacattgaa aatttttgag cctgactgtg agtattgctt tgctcaccat
    27601 tatccaacca acggcgggca atcagcatat agccgatgcc catcactaaa ataattaatc
    27661 caatcggagt aaaactgaag aaattgaagc cttcaaaacc tgattttact aattcggaat
    27721 gagcaacaag gttgggtggt gtggcaatta aggtcatcat accgctgata agtccggcaa
    27781 cacttaacgg catcattaac cggcgaggag aaattcccat tcccgagcaa atggcaagca
    27841 ccaccggaat gaaaatcgcc acaatgccgg tggaactcat aaaagatcca agcccggcaa
    27901 tacagagcat tagcagcacc agcactttgg tttcgctcgc tcccgacacc cgcatcaacc
    27961 aatcgctgac ttggttcgcc acgccggttc gcaccaaacc ttctccgaca ataaagagca
    28021 acgcaatcag caccacgttt gggtcactta agcctgccaa cacttctttg actgataaaa
    28081 tattagtcag gctaaaaaac agcattacaa ttaaggcaac cacgtccata cgcaacttgt
    28141 tttggataaa ttgccaaatc gcaaatacca ataacgccat tacccaaaac agttcttgct
    28201 taagaaaatc cggcacaaaa gagagcaacg attgcataac catatcctcc aaaatagtta
    28261 gggatagatt acaccgcttt aagcaaagaa aaaagccgat aagatcaaaa agaaaggaaa
    28321 aagcaatcac gtacggcgtg aatgctcagt acaagcggtt agttttgacg aaatttttac
    28381 aatgggcttt ccacattcag ttgttcgtca atttcagtta ccgccaaaat caggcttgaa
    28441 cgtaccattt tttccaaccg catttgctgc atttgaaaga gcatcgaatc tttattcgct
    28501 gcactcgctt tcatattcag caattcattg ataggcaaaa aatcaatatg gtgcaaatct
    28561 ttggcaaatt tcactaccgc cgggtgagaa aatgcgaact cgtcttcttc ctcattcaaa
    28621 tgctgtttga cttcaaggaa aagcgagata tcttcaaaca ctttaggtga gatcaccccc
    28681 aacccaagca acacttttaa acgtacttca atgtcagcca gcggtccttg atgttcaaac
    28741 aaggaatcca ccacggattt taacgcaaag tccgttttgc gaaacactcg ttcaatcaac
    28801 tgttcaaccg ctttggcaaa gcgttgattg gcaatgctaa ggaaaccacg cagggtggtt
    28861 tcctcactga gtttgtcgat gtaatccatc atagtgtcaa gaattattga tataattcca
    28921 ccacttgagc aatgaccgct tggtcggtta aaccggtgta tttttccacc gctgccgcca
    28981 caccgtgatt tgcaataaat tcggcaagtt cgaccgcttg tgggtcttcc tcattgcgat
    29041 attgcaatgc catcaccacg cctttgacca agttttgata cggtaagcca tattccaacg
    29101 tgccacgcaa cggtttgatc aagcggtcgt tttcgctcag tttgcggatt ggctcacgcc
    29161 ctacacggtt tacgtcatcg tttaagtacg ggttggcaaa acgtttgagg attttttcga
    29221 tatacgctga atgggcttgt gggtcgaagc cgtaacgttt aatcagcaca gcaccgctct
    29281 cttccattgt ggctttcacc tgagttttga tttcctcaat cgcaatcgcc tctttaatcc
    29341 atttcacgcc cgcttgtttg cccaagtagg cacagattaa atgcccggtg ttcagggtga
    29401 acagtttgcg ttccacaaac gccatcaggt tatcggttaa ttccattcct ttgatgtttg
    29461 gaatgtcacc tttgaactgg gtttgatcca caatccactc gctgaactct tccaccgtga
    29521 cttccaacgg atcagcaggg ttcggctcgg caggtggtac aattctgtcc accgcactat
    29581 caacaaaacc aaccactttt tcaatttctg cttgctgctc ggcagttaaa tgctcaaaaa
    29641 ttttaccttt aaagaaagtt gtgccacgca ccatattttc acaggcgata atgttcagcg
    29701 gttgggtgtt gccactttcc acacgagcca ctaacgcttt ggcgaacagt ggggcaataa
    29761 agccaagcac attcgggccg actgcggtgg taattaaatc ggtgtttttc acttgctcaa
    29821 tcacggcatt ttcgtctttc gagttaattg ccgcaatgtt tttgacaatt tccacacggc
    29881 tgtcatcgcc cacgattttc acgccgtatt gcttgttttg gttgatttgg tcgatttgag
    29941 tttggttaat atcggcaaaa gtcacgaaaa tgcgagcatc agcaagcaat ttgccgataa
    30001 agccacgccc gatattaccg gcaccaaaat ggagtgcgtt cattgatgat tgttcctcta
    30061 taaaacgtag gggcgggtcc tgtgcccgcc ctaaattttg taggataatg ggcgggcaca
    30121 ggacccgccc ctacacaaca gggtgattat ttagataata atgctaacac tttttccacg
    30181 tcatttgttg aggtcagtgt tttcattgct tgttcatcgt ccagtgcgtt ggtaattgca
    30241 cttaaaattt ggatatggtc gttgcctttt gctgcaatac cgaccaccag tttcgccacg
    30301 ccgtcttctt catcggtgaa gcgaacgccg ttcgggtatt ggcagaccac gataccggtt
    30361 ttcagtacgc tgtctttggc atcagatgta ccgtgtggca cagctaagcc ttcgcctaag
    30421 taagttgaaa ccatttgctc acgggcgaac attgcatcta catagcttgg ttgaacaaag
    30481 cccgctttaa ctaactgctc accggcaaag cggatcgctt cttctttact gttagcttgt
    30541 aagcctaaaa agacttgggc tgcttgcaat tttaaccctt cggcttgagg ttcttccacc
    30601 gggtttgcaa aattttgtgt attttgcacc gcttgttgtg gctggtggtt agccagtttt
    30661 gccactaagc tgtcatagaa cgcactgtct aagaagttag tgagcgataa gtgttgagcc
    30721 gttgccacac gttgtgttgc acgagcggtt aagtctttgt gggtaatcac cagttgtgcg
    30781 tcttgcggta agtcgttgat cgctaagttt tgtacttcaa tgtttaaacc tgccgcttgc
    30841 acttttttgc gtaacatact cgcacccatc gcacttgaac ccataccggc atcacacgct
    30901 acatagattt tgttgatgtt accatttact acgcctttgc tttctgcttt catcgcttga
    30961 gtttgtgctt gtgcatcttc caatgaggct tcaccttttt gggttttgag taagattgac
    31021 gcaatcgcaa aagtcacggc acaagaaaga atcacggagg caacaactgc aaagtgagca
    31081 cccggtgcgg tcattgctaa aatcgcaaaa atagaacccg gggaagccgg tgcgattaaa
    31141 ccgccaccta ataatgagtt ggttaaaacg ccggttgcac cgcctgcaat cactgcaaga
    31201 attaagcgtg ggctcattaa cacatacggg aaataaattt cgtgaatacc gcctaagaag
    31261 tgaatgattg acgcactacc agcggtttgt tttgctgtgc cacgaccaaa gaacatatag
    31321 gcaagcaaga taccaaagcc cggacccggg ttcgcttcaa tcaagaacag aatagatttg
    31381 cccacttctt gtgcttgtgc tgtacccaat ggcgtgaaca caccgtgatt gatcgcattg
    31441 tttaagaata ggattttcgc cggttcaacg atgatagagg ttactggcag aagaccagct
    31501 tcaaccaacg cccccacacc tgcacctaat gccgcagaga tttgggtgac gatacctcca
    31561 atgaaagcaa atgaaagcat tgccaaaatc ataccgatga tcccgaccga gaagttgttt
    31621 accaacatct caaaaccgct ttttactttg ccgtcaatcg ctttatcgaa ggttttgatc
    31681 gcccagccgc ctaatggacc tacaatcatc gcacctaaga acatcgggat ttcagaaccc
    31741 acgataatac ccattgtagt aatcgcacct acaaccgcac cacgctcacc accgactaat
    31801 ttaccaccgc tgtaaccgat taacagtggc agcaagtaag taatcatcgg accgaccagt
    31861 ttagcaaaag tctcgttcgg caaccaaccg gttggaataa acagggcggt gataaagccc
    31921 cacgcaataa aggcaccgat attcggcatc accatattcg ataagaaacg cccaaaacct
    31981 tggactttca cttttaaatt agacatagaa acctcaaaaa aggagagaaa attagggttg
    32041 aaaaaatcgt gcttaaatta gcaccacttt caaaagttac aaaaatgttt ctctcagaaa
    32101 tgtgaagtag atcacattaa atttttttgg gcagatttga aaatgtgatc tatctcacat
    32161 ttttggagta aagatggcgt aagcgtcctc gcttgtgcca agcataattc tatttttgcg
    32221 atctacatcg aaaaaataaa atgaattgct aaccctaact gttcacctat attactatca
    32281 gcataaactg cactttagag gttcataatg agtagaaaat ataagtttta tgagaagagc
    32341 ggagcctatt ttgttagttt tgcaacagtt tattggattg atctatttat tcgagaagat
    32401 tattttcaaa ccattatcca atcattgaat tattgtcgaa aacaaaaagg gatgatttta
    32461 tatggctact gcattatgcc aagccatatt catttattat ttcaagcaaa agaccaaaac
    32521 ccgtcagatt taataagaga ttttaaaaca tttacatcta aatgttgatt cagcaaatta
    32581 aaacaaatcc ccaagaaagt agaaaagaat ggttattatg gatgtttact cgggcagcta
    32641 aacaacgaaa taatgtcaag caataccaac tttggcaaca tcataaccaa ccgataacaa
    32701 tctattctca gaaatttttt gatgaaaagc taaaccatat tcacaacaac cctatcgtaa
    32761 gcggttttgt atgcgaggca tttgaatgga aatatagcag tgcaagaaat tatgctaaca
    32821 acttacctgt tttattagat attgatatat gtcagtgaac ggcacaagcg taaacgcttg
    32881 cgccatcgtt acatctataa attaactaga gaactccatc aatcccgtta ctttaccctc
    32941 atcattcact gcaattaatg agtgaatttt cagctctttc attaaatctt cggctttggc
    33001 taaaaattcg gtatcgttaa tggtttttgg attacgggtc ataatttcgt cagcggtttt
    33061 atttaggctc tccgccccga atttcgctaa tgtgcggcgg atatcaccat cggtaataat
    33121 gccttccaat ttatcgtctt gcataattac cgccaccccc atacgccctt cgttcatcac
    33181 agataaacat tcggtaaagg tggtgtctaa gctggcggtt ggcactttac gcaccatcac
    33241 atctcgcact ctgttcaata atttacgacc aagactgcca cccgggtggt agcgggcaaa
    33301 atcttcaggg cggaaatcac gggctttcat tagcgcaatg gctaacgcat cgcctaaagc
    33361 catcgttacc aaagtagagc tggttggagc tagattattc aggcaggctt cacgctctac
    33421 accaatattt aaaatcacat cggcatttcg cccaagtgtg gagtaaggat tacctgtcat
    33481 cgcaataatt ttattgccga aatttttcag gcttggaatc agtttattca catcatcagt
    33541 ttcaccactg ttagaaatca gaattaccat atcaatcggt ttcagcattc ccaaatcgcc
    33601 gtgaaaagcc tctgtcgggt gcaagaaaaa gctcggtgta ccggtggagg caaaagttgc
    33661 caccattttt ttgccaacca gccccgattt tccaataccg ccaacaacca ctcgcccttg
    33721 acaagcgagt atcatattta ccacctcatt aaattcttca gaaagtcgat ggtttagcgt
    33781 gataatctgc tgagcataga ggtttaacgt ttcttttgca ctgtctaaat gattcatttt
    33841 tacgccctta tacaactaac agctcaatcc ctttcgcttt aatctgttct ctcatttctt
    33901 gagataatcc ttcatcggta atgatagtat gaattttttc taacccacac acaatattcg
    33961 gacttttacg tccaaactta ctggagtcgg caagtaagac gattttattt gccgcatcac
    34021 acattagcga actgacttta tatacttcat tgaaagtcgt taatccaacg tctaaatcta
    34081 gtccgtctgt gccaataaag agtatgtcaa aatttgaacc aataaaagcg gattcagcaa
    34141 aataaccgtg aaatgaagct gatttctcac gaaatgtacc accggaaatc agtaagttat
    34201 aggatttatt gagtttcaaa atctcattga aaatagttaa actatttgtc attaaagaga
    34261 ggttatcaaa tgcttttaaa tggggaatca tttgtaacac tgtgctgcct gcatcaaaaa
    34321 taatagagtt tccctcatta attaactctg cagctttttg cccgatagct tgcttttgtt
    34381 gaagattaat gccggttttc tgtgcaagcg gtttatcgtc tttgtcctct ttattgattt
    34441 tctcttcccg ttcaagaatt tcttcatcaa ttaagactac actgccataa gtacgcaaga
    34501 ctttcttatc ttcttcaagt gaagttaaat ctttacgaat ggttgcacca gttagcttaa
    34561 aatagtctgc cagcacgtcc acttcggttc taccgtgctg agctaaatag gctaaaattt
    34621 gctgtcttct ttcaaaaggt ttcattgttc ctccaattta gcaaagattg gagcgagcta
    34681 atctgtgcta atgctgtttt aatttttcaa tgacatctag tagcgatgcc attcttttat
    34741 cggcaattga aaatttttca ttttgataat cggtattcac caaaaatgta gttaaattag
    34801 cggctttggc ggcaattaag ccggttacac tgtcctcaat aacaatacac tcttgttttt
    34861 caatgcctaa ttttttcact gcactcaaat agaccgcagg gtgtggcttg ccatattctt
    34921 cgtccattgc actgcatcga atcgggaaat aatcccacag ttttaagcga tcaaacaccg
    34981 ctttaatcac caccggcggc gatgaggttg caacggctaa cttgatctga ttagatttta
    35041 agaaatccaa taattcatat aaacccgtta atgctttgcc gtgttgttca atcgcagcac
    35101 aaccgtgtgc caaaatggct tcacttaatg cttggttgga aacagataac tgaaattttt
    35161 taatccaagt gtcgctcaac tcatcaatcc gcttacctct ggtgagttgt tcgcaatctt
    35221 gctcggtgat attaatacct aagtcggcta atactgcaat ttgagcctct gcccattggg
    35281 gttctgaatc aatcattacc ccgtccatat caaagatga
//
LOCUS       DANLXQ010000007        33298 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.007, whole genome shotgun sequence.
ACCESSION   DANLXQ010000007 DANLXQ010000000
VERSION     DANLXQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 33298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33298
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.007"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            8..1642
                     /locus_tag="PXD94_000270"
     CDS             8..1642
                     /locus_tag="PXD94_000270"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL6285177.1"
                     /translation="MTESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLCRNEQGA
                     AMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASHLIGTDA
                     FQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRDVQLAQT
                     SALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRNFVKIAN
                     IPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDRVTGKLD
                     TFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDVRNLKQQ
                     FDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHDPKNYIT
                     SAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAVKILLLD
                     NQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSDAIDRMF
                     NAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
     gene            1652..1870
                     /gene="ilvM"
                     /locus_tag="PXD94_000271"
     CDS             1652..1870
                     /gene="ilvM"
                     /locus_tag="PXD94_000271"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL6285178.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSAFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
     gene            2083..2556
                     /gene="nrdG"
                     /locus_tag="PXD94_000272"
     CDS             2083..2556
                     /gene="nrdG"
                     /locus_tag="PXD94_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL6285179.1"
                     /translation="MENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKA
                     CLGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKKQGLNTCLYSGLE
                     QHEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            2553..4334
                     /gene="nrdD"
                     /locus_tag="PXD94_000273"
     CDS             2553..4334
                     /gene="nrdD"
                     /locus_tag="PXD94_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL6285180.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     AKELSQGNSFFIYQSDNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMN
                     RLVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGM
                     AEAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNA
                     KWDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEAL
                     TQTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVI
                     GYLKRVSAFSSARQKEHSLRFYHREAA"
     regulatory      4391..4487
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4544..5362
                     /gene="thiM"
                     /locus_tag="PXD94_000274"
     CDS             4544..5362
                     /gene="thiM"
                     /locus_tag="PXD94_000274"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL6285181.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     gene            5355..6668
                     /locus_tag="PXD94_000275"
     CDS             5355..6668
                     /locus_tag="PXD94_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6285182.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     regulatory      6804..6900
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6968..8497
                     /gene="thiE"
                     /locus_tag="PXD94_000276"
     CDS             6968..8497
                     /gene="thiE"
                     /locus_tag="PXD94_000276"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL6285183.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLQIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNIPTVAIGGINEQSIENVMHTGVGSAAVVSAVTQAKDWQQAVKNLAKFANGGRN
                     E"
     gene            8490..9662
                     /gene="cytX"
                     /locus_tag="PXD94_000277"
     CDS             8490..9662
                     /gene="cytX"
                     /locus_tag="PXD94_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL6285184.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            9701..9910
                     /locus_tag="PXD94_000278"
     CDS             9701..9910
                     /locus_tag="PXD94_000278"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6285185.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            9918..10094
                     /locus_tag="PXD94_000279"
     CDS             9918..10094
                     /locus_tag="PXD94_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6285186.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(10135..11991)
                     /gene="sppA"
                     /locus_tag="PXD94_000280"
     CDS             complement(10135..11991)
                     /gene="sppA"
                     /locus_tag="PXD94_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL6285187.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            12094..12657
                     /locus_tag="PXD94_000281"
     CDS             12094..12657
                     /locus_tag="PXD94_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL6285188.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            complement(12728..15052)
                     /locus_tag="PXD94_000282"
     CDS             complement(12728..15052)
                     /locus_tag="PXD94_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL6285189.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLCEKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDVNQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EICKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(15052..15606)
                     /gene="pilW"
                     /locus_tag="PXD94_000283"
     CDS             complement(15052..15606)
                     /gene="pilW"
                     /locus_tag="PXD94_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL6285190.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
     gene            15826..16077
                     /locus_tag="PXD94_000284"
     CDS             15826..16077
                     /locus_tag="PXD94_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6285191.1"
                     /translation="MKIVVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTN
                     HQYDYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            16061..16408
                     /locus_tag="PXD94_000285"
     CDS             16061..16408
                     /locus_tag="PXD94_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6285192.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(16449..17609)
                     /gene="trmG/rlmN"
                     /locus_tag="PXD94_000286"
     CDS             complement(16449..17609)
                     /gene="trmG/rlmN"
                     /locus_tag="PXD94_000286"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL6285193.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYREVSNANHGK
                     VTIEYVMLDHVNDSTNHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            17869..18765
                     /locus_tag="PXD94_000287"
     CDS             17869..18765
                     /locus_tag="PXD94_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL6285194.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            18778..19521
                     /locus_tag="PXD94_000288"
     CDS             18778..19521
                     /locus_tag="PXD94_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL6285195.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(19578..20387)
                     /locus_tag="PXD94_000289"
     CDS             complement(19578..20387)
                     /locus_tag="PXD94_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL6285196.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(20380..21240)
                     /locus_tag="PXD94_000290"
     CDS             complement(20380..21240)
                     /locus_tag="PXD94_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL6285197.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
     gene            complement(21314..22099)
                     /locus_tag="PXD94_000291"
     CDS             complement(21314..22099)
                     /locus_tag="PXD94_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL6285198.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYPDPYQFKVDI"
     gene            complement(22187..22262)
                     /locus_tag="PXD94_000292"
     tRNA            complement(22187..22262)
                     /locus_tag="PXD94_000292"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22227..22229),aa:Gly,seq:gcc)
     gene            complement(22289..22375)
                     /locus_tag="PXD94_000293"
     tRNA            complement(22289..22375)
                     /locus_tag="PXD94_000293"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22339..22341),aa:Leu,seq:taa)
     gene            complement(22394..22469)
                     /locus_tag="PXD94_000294"
     tRNA            complement(22394..22469)
                     /locus_tag="PXD94_000294"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22434..22436),aa:Gly,seq:gcc)
     gene            complement(22635..23873)
                     /gene="pepT"
                     /locus_tag="PXD94_000295"
     CDS             complement(22635..23873)
                     /gene="pepT"
                     /locus_tag="PXD94_000295"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL6285199.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTFDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEHCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
     gene            complement(23893..24282)
                     /locus_tag="PXD94_000296"
     CDS             complement(23893..24282)
                     /locus_tag="PXD94_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL6285200.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            complement(24283..24543)
                     /locus_tag="PXD94_000297"
     CDS             complement(24283..24543)
                     /locus_tag="PXD94_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL6285201.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            complement(24659..24871)
                     /locus_tag="PXD94_000298"
     CDS             complement(24659..24871)
                     /locus_tag="PXD94_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL6285202.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            complement(24872..25159)
                     /locus_tag="PXD94_000299"
     CDS             complement(24872..25159)
                     /locus_tag="PXD94_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL6285203.1"
                     /translation="MEEINGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPD
                     HSSREERFLLLGRSNKDNLLVVVHCERNTNIRIISARLATKRESGQYKGVH"
     gene            complement(25322..28855)
                     /gene="mfd"
                     /locus_tag="PXD94_000300"
     CDS             complement(25322..28855)
                     /gene="mfd"
                     /locus_tag="PXD94_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL6285204.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGHSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRAKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESHRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            29016..30233
                     /locus_tag="PXD94_000301"
     CDS             29016..30233
                     /locus_tag="PXD94_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL6285205.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQAVG"
     gene            complement(30313..30819)
                     /gene="hutX"
                     /locus_tag="PXD94_000302"
     CDS             complement(30313..30819)
                     /gene="hutX"
                     /locus_tag="PXD94_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL6285206.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(30874..33282)
                     /locus_tag="PXD94_000303"
     CDS             complement(30874..33282)
                     /locus_tag="PXD94_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005715618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL6285207.1"
                     /translation="MKSKKLCPTILALSVSSILSSQATYAEETTAELSEVNVVAKEDV
                     KASIEKKNKEIIQQELIQNNRDLVRYSPDVGIVNQGRHQKGFAIRGVEDNRVGISIDG
                     VSPFPDSEENSLYKRYGNLNTSRQSIDPELARTIEVSKGADSFNQGSGNLGGGVNYRT
                     LEPYDIVRNGNKFGAFYRTGYGTRNNEWLNTFGVGYLGEKAEAILVYSNRHGHEMKSA
                     GGYTLPEDSLQTRSLRSSKQTPDDSTHNHHNYLAKFAYRFNDNHRVGVSYSGQNNKNY
                     IIEDSAVTITSFWREAEDRSKRDTVNVFYEYFPESKWISLAKIDADYQDTETSAYNYE
                     GTRAEDATDWSPAKQRKPSDNNIRIFNTELKRLNFRLDSQALEFGKWTHQLSFKASTA
                     ERDFDVLHKDSVYLSGVWVNSPDSTMMYPIKTKQHTFSLHDKIDLTHDWIATLGIRYD
                     WAKYEQQELNGLVCRNCVKTDNAKFNQLTWVTGLEKHLTEAWKVGYNIGTGFRIPNAS
                     EMYFDYRDNAAGAWMSNPNLKAERSLSQNLSLQGSGNVGLLSVNFHHTKYKDFLWEQE
                     TWDVYQAYGREFWRPVQQMQNIDSAKIYGLEVSGKWNLNSAMPIPEGWKLFGAFGYSK
                     GSMSNGADLLSIQPIKAVVGLDYEQPEGKWGVFSRFTFLGAKKAKDAKYLKTLPEKCL
                     KEDRTPNPYYPYWGDEYDIRCTEYSHETGLDTWKHLNAKAFVVDLYGFYKPTENITLR
                     AGIYNLFNRKYHTWDTLRGLNTTGGIVNSVGVRENATYGGYPGLQRYYEPGRNFSANF
                     EYRF"
BASE COUNT         9376 a         7489 c         6910 g         9523 t
ORIGIN      
        1 aagatttatc accgagagtt taaaagctca cggggtaacg acactttttg gctatccggg
       61 cggggctatt atgcctacct atgatgcaat ttatgattca ggcttagatc atttactttg
      121 ccgcaatgaa caaggtgctg caatggcagc tatcggttat gcccgtgcaa caggtaaagt
      181 aggggtttgt attgcgacct ccggccctgg ggcaactaat ttaatcaccg gtttaggcga
      241 tgccgcactt gattctattc ctgtggtagc aattaccgga caagttgcca gccatttaat
      301 cggcactgat gccttccaag aagctgatgt actaggtttg tcactcgctt gcactaaaca
      361 cagttttatt gttcagaata ttgaggagct ccctgaaatt ttagccctcg cattccaaat
      421 tgcacaaagt ggcagacctg gtccggtgct ggtcgatatt ccacgtgatg tgcaactagc
      481 tcaaacttcg gctctgccta tcgtttatcc taaagaacct gccgatgcac aagatcccaa
      541 caaattagtg gttgccaaac aattattagc caccgccaaa cgcccggtac tttatgtcgg
      601 cggtggcgtt agtatggctg atggtgtaca agcggtcaga aattttgtaa aaattgcaaa
      661 tattccgtct gtttctacct taaaagggtt aggcactatc gatccaaccg atccgctcta
      721 tatgggaatg atcggtatgc acggcacaaa agcagcaaat tacgcggtac aagagtgtga
      781 tttactcatt gcctgtggtg cccgctttga tgaccgtgtt accggcaaat tagacacttt
      841 tgcccctcac gctaaagtga ttcacattga tatcgacaat gcggaaatca acaagctccg
      901 tcaagcacaa gttgccttaa aagggaattt gattgaagcc gtgaattact tggctcaacc
      961 gttagatatt gaaccatggc gtgaagatgt gcgtaattta aagcagcaat ttgactttca
     1021 atatcaagaa aatagtggtg atggcaatat tgatgccgtg gcgttattac acacgctttc
     1081 acagcggaaa gccaaaaaca gcatcgtcac taccgatgta gggcagcatc aaatgtggtc
     1141 ggcacaacat ttaacttatc acgatccgaa aaactatatc acctctgccg gttttggctc
     1201 tatgggtttt ggcttgcctg cggcgattgg ggcaatcaaa gctcgagcgg aagatcaggt
     1261 tattttaatt accggtgatg gctcgattat gatgaatatt caagaactcg gcacgcttaa
     1321 aagagctgat ttagcggtca aaatcctatt actggataac caacgtttag gtatggttcg
     1381 ccaatggcaa tcgctgttct tccacgggcg acacagccaa accattttag atgataaccc
     1441 tgattttgtg ttgttggcga aagcctttga tattgacggt gagcgaattg agaaagcgag
     1501 cgaagtgtca gatgcaatag acagaatgtt caatgccaaa ggggcttact tacttcacgt
     1561 ttgtattcca caggaagata acgtttggcc actggtaccg ccgggggcgt gtaatgccga
     1621 tatgttagac gatgaggtat aggagaataa gatggaagaa tatcaattaa cgcttaaagc
     1681 taacaaacgc cctgaaacct tagagcgtat tttacgggtt atacgtcacc gtggttttga
     1741 ggtgaaatgc ttaaatgtag aatcaacaga cagcgccttt aacctaaatt ttacattagc
     1801 aagtaaacgt ccactctctc ttttaagcca tcaattagaa aaactgtttg atatcattga
     1861 aatcaattaa tcagactttt cattcgccgt gtaaaaaaat acacggcttt tttatgctta
     1921 aaatttatca ttcgctatcc ttttgatttt attcatttct tcaaatttca actctttttt
     1981 ttcacttatt gctctttatt ttcccattca acagatctaa catactatat atagtgttaa
     2041 ataatgttaa aaaacacaat atattgtgtt aatgaattag taatggaaaa tctcagattc
     2101 agctcggaac aaattgtttg gcaagaagtg ccgaatgaga cctcacttgc ttttctgatt
     2161 acaggctgcc cgcttggctg taaaggttgc catagtgcgg atagctggaa agcctgtctt
     2221 gggaaagagc ttagcagtga atatctctct gaacgcctaa aacgctacca aggcttaatt
     2281 agctgcgtat tgtttatggg cggagaatgg gaagcagaaa aattactgcc gctgttgcaa
     2341 ctggcaaaaa aacaggggct aaacacctgt ctttatagtg gtttggagca gcacgaaatc
     2401 gccgagactt tattacacga gctaacctac ttaaaaaccg ggcgttggat tgccgaacga
     2461 ggaggattgg aaagtttaac caccaaccaa cgctttactg atttacgcaa taaccaaaat
     2521 cttaaccatc tttttattcg aggagcttca atatgattcg cttacaaccg acacaattag
     2581 acggaaaact acatttcatt aaccaatata ttcaagccaa aaatgccgca gacggttcaa
     2641 aaatggacgc caacgctaac gtcacgcaaa aaaacatagc gacaatggaa caggagctaa
     2701 tgaaagattt ttttgtgcaa atcaaccgag caaaagtgag tgaaaaaatt gcaaaaattt
     2761 ttggaaaaga aaccgcttgt gaatatttac gtcaaatcga agcacacgaa atttacgtac
     2821 acgatgaaac cagcctaaaa ccctactgcg tttcagtcac tatgtaccct ttcttgcgtg
     2881 atggtttgac taaactcggc ggcgaatcca aagccccgaa acatttagcc tcattctgcg
     2941 gttcttttat caattttgtc tttgcagtca gttctcagtt tgccggtgca gtcgccacgg
     3001 ttgaatttct gacctatttt gactattttg cccgcaaaga ttacggcgat aattacttac
     3061 agactcatcg ttcagatatt gaaaaccacc tgcagcaggt tgtgtatagc atcaaccaac
     3121 cggcagccgc tcgtggttat caaagcgtct tttggaatat ttcagtgtat gacaaattct
     3181 attttgaagc gatgtttagc cgttttgttt ttcctgattt tgagaaacca aattgggaaa
     3241 ccacttcaaa attacaactt ttctttatgc aatggttcaa taaagagcga agcaaagcga
     3301 ttctcacttt ccctgttgtg accgcagcaa tgcttaccga aaagggcaaa tgtaaagatg
     3361 acgagtttgc caatcaaatg gcaaaagagc tatctcaagg caattcattc tttatttatc
     3421 aatcagataa tccggattcc cttgcttctt gctgccgctt gagaaatgaa atcagcgatc
     3481 atagcttctc ctattcatta ggggctggag gtgttgcgac aggttcaatc aatgtaatta
     3541 ctattaatat gaaccgtttg gtgcaagata accgtgattt aagcgaagaa gtcagcaaga
     3601 tccataaata tcaatacgct taccgcaaac tgatggaaga gtatttagct gccggtatgt
     3661 tgccggttta cgatgccggt tttatttcac tgcataaaca attcctgact attggcatta
     3721 acggaatggc agaagctgct gaatcacaag gtttaaccgt aggttacaat ccagactata
     3781 ttgattttgt gcaatctcgg ttaaaaatta ttttccaagc taaccaagca gccggtgaaa
     3841 agtatggggt aaaatttaat accgaatttg tgcctgctga aaatttaggg gtcaaaaacg
     3901 ccaaatggga caaagaggac ggctatttcg taccacgaga ttgctacaat tcctatttct
     3961 atgtggtaga agatgaagaa attaatgctt tggataaatt cttactgcac ggcagagagc
     4021 tgattgaatg gctggacgga ggctccgctc tacacttgaa tttagacgaa gccttaaccc
     4081 aaaccggata tcgctccttg ctggataccg ccgctaaaac cggctgcaat tatttttgta
     4141 ttaacgttaa aatcacgatt tgcaacgaat gtagccacat cgacaaacgc acgttgcaca
     4201 gttgtagcaa atgtggctcc gataatattg attacggcac tcgggtaatc ggctatttaa
     4261 aacgagtctc cgcctttagt tccgcccgcc aaaaagagca cagcttgcgt ttctatcacc
     4321 gagaagcagc ttaattgaaa actgcagttg tagcccttta caaccgcttt tttagtgatt
     4381 atattaaagc ctcatttagt cggggtgctt gcctaagggc aagctgagaa atacccgttg
     4441 aacctgatgc agttagcact gacgtaggaa actaaaggcg taaacccatc tattccccgt
     4501 tggtgttggt gcgttgtttt tcaatcatat taaggaataa acgatgacca catcactccc
     4561 ctatcactcg cattatttgc aaaaaattcg acaaaatcga ccgcttgttc acaacattac
     4621 caacattgtt gccgctaatt tttctgccaa cggcttattg gctcttggtg cttctcccat
     4681 tatggcagct gctattgagg agatggaatc cgtgccggca ctcagtaacg ccgtggtgat
     4741 taatatcggc acgcttatcg gcaaagaagt agaagcaatg gagcttgctg gcaaaaccgc
     4801 caaccgttta ggtattccgc tcattcttga ccctgtcggc gttggagcaa cacctttccg
     4861 ccgtcaaaca gttgaaacct tagtcgacca aattcacttc accgccattc gtggaaatgc
     4921 gggggaacta gctaccctcg ccggtgtaga atggcaagcc aaaggcgttg atgccggcga
     4981 aggctcgcat aatgtggttg aaatcgccca acagattgcc aaacgttacc aaaccttagt
     5041 cgcaatcagt ggtgaaaccg atgtggtaag ctctggctca cacacagctt tattgcacaa
     5101 cggcagcccg atgttcccca aaattaccgc ttcaggctgt ttattaagtg cggtatgcgg
     5161 ggcattctta gccgttgccg acaaagagca ctatttcaac gcacttattg aggcttgcgc
     5221 cgcttatgcc gttgcaggcg aagtggctgc tcaagcactc aaaccaactc aatacgggca
     5281 atttgccgtc aatctgctgg atacccttgg ggcattaacg cctgaaaccg ttgagcaata
     5341 tgtgagggtt gaatatgtct aaaactcact caaacccaac taaagtggcg gttgcctcaa
     5401 tggtcggcac ggcgattgaa tattttgata actacatcta cacgatggca gcagtgctgg
     5461 tgtttaatgt gcagtttttt aatagcgatg atccggtttc caaccaatta atgtcgcttt
     5521 ccgtactagc tcttgccttt ttcacccgcc cgatggggtc gattttattc gggcatttcg
     5581 gcgataaata cggacgcaaa caaactctga ttgcctcgct cttattaatg ggcttatcta
     5641 ccgtagcaat cgggcttttg cctaattatg ccgccattgg catttgggca accgtgttac
     5701 tctgcctctg tagaatcgga caaggtttag gcttaggcgg cgaatggggc ggagctgctc
     5761 tcgttgccac tgaacacgcc ccgaaacaca agcgggcatt ttttggtatt tttccgcaaa
     5821 tgggcgcccc aatcggcttg ctgttggcaa acggagcgtt ctacttggtg aatttaattt
     5881 acggtgaaca aaccttcttg gattgggcgt ggcgaattcc gtttgtcgct tctattgtga
     5941 tgatcgccat tgggctttat atccgaatta agatcagcga aagcccgatt ttccttaaag
     6001 cggaacaaca aggtaaaaac cgccataccc cgcttaaaac tttgtttagc caacacctca
     6061 agccttttat gatcggcgtg ctgattgcca ccgccggcta tgtgttgttc tacattttag
     6121 ttgctttcac cccgattttc gtcaaaagcc cggtggcagt ttctcaagca ggacacgcta
     6181 caggcttagg tttaccggta aatctctaca cccaatattt gctgattacc tccgtgattt
     6241 tcggcattgc gattttcttc tccggcattt attcagacaa aatcggacgc cgtttactgc
     6301 tgttacttgc cacaggagca accgtagttt acggcttaac aatgccgttt ttcctcgaaa
     6361 acggcacacc gacttcgatc tttattttcc tcaccattgg aatgatctta atcggcttaa
     6421 gcttcggccc gatggcagta attctacccg aactcttccc aacagaagta cgttacacag
     6481 gggcttcgct tgcctacacc gctgccggta ttttaggagc tagcgtgttc actattattg
     6541 cggtcaaaat caatgaaaat ttcggcttat tcggtgtagg cggctattta tcggttgcct
     6601 cattactgag cttctgggca ttgtggcaag tccacgaaac caaagatttg gaattagagg
     6661 agatataaaa aaccactcac ttaacgacgg ggcaagttac caccttgccc caagatgagg
     6721 gaatgcaaat ttccctctaa tagacttcac aacggatatt ttgcattttt ttataaaaaa
     6781 taaccgcttg taagctatac taaaaacgct tgtcggagtg ccgtaaggct gagatcgcaa
     6841 cagcgagatc cgtggaacct gtaggttagt acctgcgtag ggaacaagtt ttacccaaat
     6901 tcagattttg ggatcattac cacatctctg gcaagcaaaa ccctaagcat attatgagga
     6961 ttttgcgatg tctaattatt caaactctcc ataccaacct aacgtcattt ggacggttgc
     7021 cggctcagat tcctgtgccg gtgccggtct gcaaaccgat ttacatacct ttcacgattt
     7081 taacctagtg ggttgttcag tagttacttc tgtaacggct caacatccgc acggtgtgct
     7141 gtgtgtaacg cctgttgatg atcacacctt ccgccaacag tttgaagcct tgctggttca
     7201 aggctacccg aacgcaatca aaatcggctt actctgctcg caagcacaag tagagatctt
     7261 gtgtgaatat atccaaaaaa tccgtgcaga aagctcgcat tattgttatg tggtttacga
     7321 ccctgtggca gtggcaagca gtgggcaggc gttatccgac agtattttat tgcagatagt
     7381 gcaacaaaaa ttgtatccgc tggtggattt aatcactcca aacggcactg aactcgcttt
     7441 attaagcgac acagaaatcc agacctttgg tgatgtgaaa accgctagcc acaaactgtt
     7501 tgcacaggga atcaaagccg tgttagccaa aggtggacat tttgaatggc tgggggaaac
     7561 ggtaagagat tatctgctga ctgcaaatga aatttatgcc tttagtcacg cccgacagca
     7621 aagtgtgaat acccatggca caggttgcac ctatgcttca gcggttggag cgatgttggc
     7681 aaccggcttt gatttagctg atgccgtcac tgttgccacc gcttatttgc aaaaaggctt
     7741 atcggaaaaa caaggcagag ggcaaactgc tttaagttca ctttgccaca ccggctaccc
     7801 tgatgatatc gctttcttcc cacaaaccga gctgatttct gcccctctta aattgccgaa
     7861 agagcctttt gctccaacgg aatatcaact tgggctttac cctgttgtag atagcgttaa
     7921 atggcttgaa cgcttaattc ttgtaggggt aaaaaccttg caaattagaa tcaaaaatcg
     7981 accgcttgca gagattgagc aagaaattgc ccattcggtc gcactggcga aacagcacaa
     8041 tgtgcgtttg tttgtaaatg attactggca gttagccatg aagtatcagg cttacggcgt
     8101 gcatttaggg caggaggatt tagccaccgc cgatttaaat gcaattcagc aatctgggtt
     8161 acggcttggt gtttcaaccc actgctactt tgaaataatg cgggcattgg cggtgaaacc
     8221 gtcttatatc gcttttggcc acgttttccc tactcaaacc aaagtaatgc cgtctcaacc
     8281 acaaggctta acaaatttag ccaaatatgt tgctctcgtt gcaccgctta acattccaac
     8341 tgtggcgatt ggtggaatta atgagcaaag cattgaaaat gtaatgcata ccggtgtcgg
     8401 cagtgccgcg gtggtaagtg ccgttaccca agcaaaagat tggcaacaag cggtcaaaaa
     8461 cttagcaaaa tttgcaaatg gaggccgtaa tgaatagaac tcttagtacg ctaaacagtg
     8521 gtttgatttg ggcgggagcg ggcattagtg ccagtgcaat cgtggttggc acttggattg
     8581 ccccacttgg ctggcaacaa ggcttgctgg cgatagtgct agggcattta ttaggcggca
     8641 ttttgttttt tgctgccggc ttaattgggg cgaaaaccgg caaaaatgcg atgcaaacgg
     8701 tgcaaatttc ctttggcaga ggctcggtgt ttttctccct tgccaatgtg ctacagcttg
     8761 ttggttggac agccattatg atctatacag gggcagagat cagtaatgcc cttagccttg
     8821 ccttatggga atattcggct ttctcgcttt gggcaatcgt gcttgggttg ctgattgtgc
     8881 tatggttatt taccggctct aatcaggtcg gaaaattcaa gttagtgaca ttgatagcga
     8941 tgtttttgct caccttatgg ctaagcatta aggtattgaa tgggcaggaa aattttcctg
     9001 tttcgggtga aatgccgttt agcaccgcag tggaattagc aactattatg cctctttcgt
     9061 ggctaccttt ggtatcagac tataccgcta aagcgaaaaa gccttttgcc gcgacccttt
     9121 cggcgaccat aggctatttg atcaccagtg cgtggatgta tgcgttaggt ctgggtatgg
     9181 tgctatttac cgggcaaacc gaaattgccc ctatgttact aatgacagga atgagcctta
     9241 tcggcattat ggtgatgatt ctttcgactg tgactaccac gtttcttgat gcctattccg
     9301 ccggtgtcag tgccggcaat atcacggcaa aattcaaaga aaccccaacc gccattctag
     9361 tcacaatcat cggcacgcta ctggcaatca gtttaccggt tacccaatat gaaaatttcc
     9421 tgctgctgat aggctcggta ttcgccccga tgattgccgt gcaaattgcg gatttctttg
     9481 tgttaaaacg ccaccaagca cagcctaatg ttgattgggt tgctgccgga ttatggcttg
     9541 tcggcttcgt gctataccac cttctctctt accaccaagt gcatttacta ctcggcttaa
     9601 ccattccagt gatgttagtg atttttctca gcaccatttt tgtgaggagg ttcacaaaat
     9661 agaaagataa gcggttcaat tggagtgaaa atttgctaaa attgagtcgc ttgttttaaa
     9721 caatgactgg gtagtaacac cacaagctca acttgcctac cacaccatta acggtaaagc
     9781 cgatgaggaa cggttaaatc ttttagttgc ccgtgccggc gtaagagtag caaaaggctt
     9841 tgccttgaat aatggttgga atctgcaacc ttatgcggaa gtaaacggca ttgctgaaaa
     9901 aggccaatag tacgaaagtg caagtaaacc aatatcagtt tggagttgcc gaaaacaagg
     9961 gcagagtgca aggttcactt ggcttgagtg caggaaacgg taatcaccgt ttaggtttag
    10021 aggcatcagt tgcttcaggt aagaaactga aacaaccatt taatgtatta gtaaattatc
    10081 gttatcaatg gtaatttaag agggcaggtg aaaacctgcc cttaaattca ataattaatc
    10141 tatcttccca caatttaaac agtaaagata catctgtttc ggatcgttaa aacgtgaaag
    10201 agtctctagc tgttgttgcg gttgcttcaa cagcggtaaa tcaagccaat ttgccacgct
    10261 aaattgtaat tgagctttga aatcacgggc aagcgtgcca aataagctgt catcttgctc
    10321 cgcaaaccat tgtaagccga actgttcgac tttaggttgg ttttccgctt cacgtttttg
    10381 gttgatcaat tcagataacg cattatatgc cacattaaaa ttgcccaact catcaaccaa
    10441 tccgtgtttt aacgcatcag ttcccagcca cacttgccct tgagccactt tatctaccac
    10501 ctcttttggc attttcctgc cacgagaaac cagctctaaa aaacggtcgt agccgttttc
    10561 aatcccaatt tgcagcactt ctgcctgctc ttgcggtagg ctttttaaac cggcttgttg
    10621 tgctagggca gaagtcgcta tgccgtcttc gctcacaccg agatttttag cggttttttc
    10681 aaaagttact gctaaaccga aaataccgat agagccggta agcgtattcg ggctagcaat
    10741 aattttatcg ctggtagccg caatccaata accgcccgat gctgccattc cgcccattga
    10801 agcaaccacc ggtttgccgg cttgttgaaa ggcttccact tcttggcgaa tcagttcaga
    10861 cgccacagca ctgccgccgg ggctgttaat gcgtagaatt aagccttcca catttttgtc
    10921 atctcgagct tgttgcagta attttaccag cgtctcgctg cccacattca tttcgtcact
    10981 ttcaccgcca gcaatcgccc cttccacatt cactaccgca attttcggag cattcacttc
    11041 tgcaaagcgg tcgtttagtt cagtcatata atctaaaaat tcaatctggt tataactgcc
    11101 ttcgctgttt ttgccaaact gtgcaatcag tttatcccga ctttcttggc tggacactaa
    11161 ctgactaacc catttttgat ttaatgccag ttgagcatca tctcctttag cttgtttata
    11221 tttttcaatg taattggcag gcgataaatc cagtgcttgc ggttcaatgt tgcggttttg
    11281 agctagtgtt gccacagttt gttgccaagt gccgtttaac cagcctcggg cattttgttt
    11341 cgcctcctca gacatatcat cacgcataaa cggctcaacc gccgatttat aagtaccgac
    11401 acggaaaata tgcggttcag cctcaatttt atcgagcaag gttttgaaat aagtgttgga
    11461 ataatttaag ccttttaaat ccaccgcacc gactttattg agataaattt catcagcaaa
    11521 ggaagcaaga taatattgtt tttgcgagta gtcttcgccc actgcaatca ccggcttgcc
    11581 cgattgtttg aagttgttga tttcattgcc gataaaatcc aatgagctga aatcagcact
    11641 ttgtagcttt tgcagatcta acaccaagcc ggtaattttc gggtcatttt tagcgtggct
    11701 aatcgcttgc accacatcaa aagtcgagat tttaaacggc tcggaactac ctaattccga
    11761 ttgcaccaag cggtagaaat cagtgaattc ttcccgatta tctgctaaat aaccatctaa
    11821 atttaaacgt aacgcccctt gattgaacac cggcttttgc gtgctgccag aagagctgat
    11881 aaatgcgacc accggcacta atagcaaaat aaacagaata aaaaagagat taatgacaca
    11941 ctctctaata caacggaaga ttcggtaaaa tgcttttaaa atcgttaaca tcggaaattc
    12001 ctaaaaataa actcagtcgg cacagtataa agcaatcgct aaatgaagaa aatatgctat
    12061 tcttgaacgc aaaaaattaa caaaggaacg cttatgcaaa tcttggattt attacaccat
    12121 cgccgttcaa ataaaaaatt tggcgataaa gccccgaatg cagaacaact ggaacaaatt
    12181 ctaaaagccg gcttgcgagt gccggatcac gggcggttaa agccttatcg ctttgtggtg
    12241 attgaaaaaa gcggtatgcc acagtttggt gaatgcttaa agtctgcggt agcagaattt
    12301 gatatgggcg aggaacgctt aaaaaaagcg gaaaaattag ctaatcaagc tccaatgatt
    12361 atcggcgtgg tagcaacatt agacgacagc atcgccaaag tgccaagttg ggaacaaatg
    12421 attaccgcag gctgtgcaac ttatgcgatt cagttagcgg caaatgcaca gggctttgat
    12481 acggtttgga tcaccaataa atggattaac ggctctgctc ttcgctccgc ttttggttgc
    12541 caagaatctg ataaaatcat tgccttaatt atgcttggct caccggcaga gggcgagcaa
    12601 attgcattag cccctgaaag tgaaagttta gaggggtttg tacgttatct taagtaagca
    12661 ggttaacaga taaaagttag taataagggc gaaaaatatt tcgcccttgc ttttatctgt
    12721 tttcgcctta cttcttccag ccttttaacg catcggcaaa ggcattgtta ccaaaagcgt
    12781 tgcgattgtt gctttgcaag cggtcatttt tctgcgtatt tttgcaaatt tctgctgaat
    12841 ttcgaccgct tgttggctct tttttatcgg ttggtttgtc gtctaaacgc atcgtaaggg
    12901 caatacgttt gcgggcagcg tccacctcaa gcactttgac tttcaccaca tcaccagctt
    12961 tcactaccgt gtgcggatct tcaacaaaag agtttgagag cattgaaata tgcaccaagc
    13021 catcttggtg tacgccaata tcgacaaaag ccccaaagtt tgccacattg gtcactgtgc
    13081 cctccaaaat cataccggct ttgaggtcgg taatttcttc cacgccgtcc ataaacacgg
    13141 cggttttaaa ctctccacga gggtcacgcc ccggtttttc taactctttg aaaatatcgt
    13201 tcaccgttgg taaaccaaac tgctcatcca caaaatcttt ggcgttgagc gagtgaattt
    13261 tgctgttgtt acccattaat tctgcaagtg ttgaggtagt tgcctgtaag attttttcta
    13321 ccaccgggta agcttccggg tgaacgcttg aggcatcaag ggcatttttc ccaccgataa
    13381 ttcgcataaa accggcacac tgttcaaagg ctttcggccc taaacgaggc acttttttca
    13441 gctctgagcg gctggtaaaa cggccgtttt cgtcacggaa tgccacgata ttttgtgcca
    13501 atgttttggt cattcccgcc acccgagcca gcaacggagc ggaggctgta ttcagatcca
    13561 cgcccactgc gtttacacaa tcttctacta ccgcatcgag tttacgggca agttgggatt
    13621 ggtttacatc gtgttgatac tgcccaacac caatcgcttt tggttcgatt ttcaccaact
    13681 cggcaagtgg atcttgcaaa cggcgggcaa tcgaaaccgc tccccgtaat gaaacgtcta
    13741 aatttggaaa ctcgttagcc gcaaattcag aggcagaata gaccgatgcc ccagcctcac
    13801 tcaccaccac cgtttgcggt ttattctctt tgatttcttt aatcaccgct ttggcaaaac
    13861 gttcggtttc acgagaggcc gtaccgttac caatcgcaat cagttccaca ttgtgttgtt
    13921 tgattaagct gaaaatcgca agctgtgcct ccgcctctcg cccagtgtga gggtaaattg
    13981 tggcggtatc cagcaattta ccggtgttat ccaccaccgc cactttcacg ccggtacgca
    14041 agcccgggtc tagccccatt gtatttctcg caccggcagg ggctgccatt aataatgccg
    14101 agagattgcg ggcaaacaca tcaatcgcct cttcttcagc tttttcacac agggttgcca
    14161 ttaattcggt ttctaaatgc agagctgctt tgattttcca cgtccaagca atcacttgct
    14221 cacgccattt atctgctggt tgattattaa aaatcacgcc caaatgctca cgaataattt
    14281 cttcacaatg gctggatttt gtgccttctt ctgcagtcgg atcggcattt aatgaaagtg
    14341 ataacacccc ttcatttcgc ccacggaaca tcgccaaagc acggtgggaa ggcacgctgt
    14401 taaacggctc gctatgagca aaataatcac ggaatttctc gccctcttcc tctttgccgt
    14461 caatcacttt cgattccaac gtggcgtaag cggtcaaata ttggcgaagt tttgcaagta
    14521 gctcagcatc ttcgctaaaa cgctccatta aaatatatct tgccccgtct aatgcggcct
    14581 tggtatccgc aacgcctttt tccgcatcaa caaagtttgc cgctaaggtt tcaggatctt
    14641 gtttcggatc attccataaa ctgtccgcca atggctctaa ccctgcttca atcgcaattt
    14701 gccctctggt gcgacgtttt ggtttatacg gcaaatagag atcttccagc tcggtcttgc
    14761 tttcactgct tagaattttg gcttttaatt catcggttaa tttgccttgt tcttcaatcg
    14821 acttcaaaat ggtttggcgg cgttcgtcca tttcacgcaa ataaattaag cgggtttcaa
    14881 aatggcgaag ttgggtgtcg tctagcccgc cggtcacttc tttacggtaa cgagcaataa
    14941 atggaatggt attgccctca cctaatagcg tgatggctgc aagaatttgg tgtgagccaa
    15001 cgttgagttc gccggcaata atttggctga tttttagatt taagtcagtc attagttttc
    15061 cttcaataaa tttgctcgag gtttatccaa tttctcaagc tcggtcaatg tttcggcaat
    15121 tttttgcaga ttcggctctt ttttcgcaca taacaccata ttttctaagc tatccgcttg
    15181 gtgatagtag ggtgctttgc ttttgacagc ctgctcaaat tgctgataag cctgttgaaa
    15241 ttgaccctgt ttacataaaa aagtgccgta attattcagc acatcagggc ggttttgact
    15301 caatttcagt gccgtttggt aggctttttc ggcattttca gcatcacctg tttgttggta
    15361 ataataggca agcaccgaat aagggaggta atctttttca tcgtgagcca aggctttatc
    15421 aatattctgc ttagcttttg ggaaatcatt ttgctccaga taagctaaag ccaaattaat
    15481 acgggctttt accgcttccg agcggttaaa ttcgacttca gaggaagaag ggggaagaga
    15541 agaacaagcc gctaataaaa aaatcagaat acaagcggtc atttttggaa aaaattttgc
    15601 aaacataacg ttctcctaca acaaagggct taattttact gttccatcct cgctaagact
    15661 agcgattttt tgcgaagaag aaaaattttt cgttttcatt tttcgacgca gatcgcaaaa
    15721 ataataatgg ggtgttgtgc tagggtaaat ggtaggaata cactctctat ttaatataag
    15781 aatttcattg aaaaaactta taaaacttat aaaatagagg caaaaatgaa aatagtggta
    15841 ttccagcgta aagccttaaa gcaattacga aaaatcccaa ctggtgcaga aattcgccga
    15901 aaatgtgatg atttagctta ctttccctac tgtgccaata ttaaggcatt aacaaatcat
    15961 caatatgatt atcgctttcg ggtcggaaat taccgtattt ttttcaatat tgaaggcgaa
    16021 acgatgaata ttgtgtctat cgaagaggtt aaaaaacgag atgaaagaac ttactaatat
    16081 tcaatattta aataatgaag aaggaaaacc tgcctttgcc gtacttccga tagcaacact
    16141 tgaatggctt aagcagaaag caaatattaa aaccgcaata gagaccggta ttccatctgc
    16201 tgttgctgga attgcattag ataataacta ctcagctctg cgtgcttggc gggaatattt
    16261 aggtttaact caagaagagg tggcacaacg cttggggatt tcgcaatcag cttatagcca
    16321 acacgaaaat gcaaaaaatc tacgaaaaac gacccgcata aaaattgcct atgcgttggg
    16381 tttaaatgaa gagcaattag atttctaata acaaaaaggc aggaacctct gccttttcac
    16441 tattcagatt aatgggtttt aaccgcaata ccttgcccga atttacgttt ttcaagggtg
    16501 cgtttggtgc ggtcaatcac atcgccagca agctgtccgc aagcggcatc aatatcatca
    16561 cctcgtgttt tacgcactgt taccgtgaaa ccgtactcca tcaaggtttt ttggaaacga
    16621 tctacccgag agttagagct tttaccataa ggagcttctg ggaacgggtt ccacggaatc
    16681 aagttaattt tacacggcgt gttttttaat acttcagcaa gttggtgggc gtggtttgtg
    16741 ctgtcattga catgatctaa catcacatat tcaatcgtga ccttgccgtg gttggcgtta
    16801 gaaacttcac ggtatttatt caccgaatcc atcaacattt tgatgttgta ctttttatta
    16861 atcggcacta attcatcacg cagttcatcg ttcggagcgt gaagtgaaat tgccaacgcc
    16921 acatcaattt gctcacgcat tttgtctaat gccggcacca cacccgaagt ggaaagcgtg
    16981 acacgacgtt tagataagcc gtaagcaaaa tcatctaaca tgatttccat tgccggaatc
    17041 acgttattca tattcagcaa tggttcaccc attcccatca tgaccacatt ggtaatcggg
    17101 cgaacaccgg tcacgccaaa attgccgata attttagaag cacgccacac ttgcccgata
    17161 atttccgcca cgcttaagtt acggttaaag ccttgttgag cggttgagca gaaggtacaa
    17221 gccaaggcac agcctacttg tgaagaaaca cacagggttg cacggtcatc ttcaggaata
    17281 tataccgtct cgatttgctg atcgcccact tgcattgccc atttaatcgt accgtctgca
    17341 gaacgttgtt ctaccgccac ttcaggggct ttaatttcgg caatacgctt taatttttca
    17401 cgcaatactt tattgatatt gctcatatta tcgaaatttt cttcgccaaa atggtagatc
    17461 catttcatta gctggtcggc acggaaaggt ttttccccca tttctgcaaa cagttctcgc
    17521 atttcttgac gattaagatt taataaattg attttttctt ttggttgttc agcagaccca
    17581 cagcctgcat tggtaatcag ttctgacata aagttgaaag cctcgttatt acacggatta
    17641 cggcaaaagg ttagggcaaa tgcccttatt ttgcgattgg attttaaagc gtgcaatttt
    17701 actggctctt gagggattag gctagtaatt tttaatcaaa agagttgaat tgcctgagta
    17761 aacttatcaa gaataacctt ataaaaacaa gactatttac atcattccca atgttctata
    17821 gaatagcttt cgataattct cattctcaat aacaaaagga ggctaaaaat gaaactatca
    17881 acaattaaaa aagcattggt attaaccttt ggtttaagtg cagcgatggc aatggcagag
    17941 ccgtttaaag tagttaccac ttttaccgtg attcaagata ttgcacaaaa tgtcgccggc
    18001 gataaagcca ctgtggaatc cattactaaa ccgggtgctg aaattcacga ttatcaacca
    18061 acaccaaaag acattgtaaa agctcaaaag gcagacttag tgttatggaa cggtatgaac
    18121 cttgagcgtt ggtttgaacg tttctttgaa aatgtaaaag gtaaaccggc ggttgtcgtc
    18181 accgaaggca tcaccccaat gccgattacc gaaggtgaat ataaaaatct accaaatccg
    18241 cacgcttgga tgtccaccga taacgcatta atttacattg aaaatattcg tgcggcttta
    18301 gtaaaatatg acccacaaaa cgcagacacc tataatgcca atgcaaaagc ctatgctgaa
    18361 aaagtaaaag gtattgctga gccactccgc caacgtttag cttttattcc tgagcagcaa
    18421 cgttggttag taaccagtga aggggcattt agctatttag cgaaagacta caacttaaaa
    18481 gaactttatt tatgggcaat caatgcggaa gagcaaggtt cgccaaaaca agtgaaaaaa
    18541 gtgattgatg gtgtaaaagc caacaaaatc cctgttgtgt ttagcgaaag taccgtttcc
    18601 gacaaaccgg caaaacaagt ggcgaaagaa accggtgcat tatacggtgg cgtaatttat
    18661 gtggattcac tttcagagaa agatggtccg gttccaacct atttagattt gcttaaagtg
    18721 actatcggca ccattgtcga agggttcgaa cgaagcaaaa aataataaga tttagcaatg
    18781 acaactgttt cagcctccat ttccgtcgaa aatttatcgg tccgttataa taacggacat
    18841 atcgctttgc aagatgtctc gtttcatctg gaaaacggca caatttgtgc attaatcggc
    18901 gtgaatggag gtggaaaatc cactctgttt aaaagtttaa tggggctggt taagccatta
    18961 accggctctg ttaagctgag tcagctcccg attgctaaag ccctcaaaca aaatttggtt
    19021 tcctatgtgc cacaaagtga agaagtggac tggcaatttc cagtttcggt ttacgatgtg
    19081 gtaatgatgg ggcgatacgg ctatatgaat tttctacgcc gcccaagcca aaccgataag
    19141 ctcaaagtag agcaagcaat gcaacgcctt gatgttttac atctcaaaga caggcaaatc
    19201 ggcgaacttt ccggcgggca aaaaaaacgg gtatttttag cccgtgcctt agctcaagaa
    19261 agtaaaatca tcttacttga tgagcctttc accggtgtgg atgttaaaac cgaaaatgcg
    19321 attgttgagc tacttaaaca gcttaaaaat gaggggcatt tgattttagt ttccacccac
    19381 aaccttgcca gcgtaccaag cttttgcgat caggttttga tggtcaatcg cacgctactg
    19441 gcaaaaggta aaacggaaga aatctttaat aatcaaaatt tagaacgggt gttcggcggt
    19501 ttattgcatt atcaaaagta gtgtttcagc aacaaatcac aaacaaatag ggaggcaaat
    19561 gcctccctat ttttgtatca atgaatctta gaatatgcaa aactgaggaa aaaaatggcc
    19621 gcttgcacga gaataatcgt cggccctgtt gcggcatcaa tatggtagct aagcaacgtg
    19681 ccgaataccg atgaaaatat cgcaacgcct aaggcaatca gtaacatact atcaaaccgc
    19741 ttagtcagca ggtaagcagt gatccccggg gtaatcaaca ttgccaccac taaaatcacg
    19801 cccaccactt gcattgcact gataatagtt aaagcaagca aggaaagtaa accataatgc
    19861 aataatctaa ccggtaagcc tgctactttg gcttgattaa catcaaagca atagagcaag
    19921 aagtctttgc gtttcgccac catcaccaca aaggtaaaga ggcttatgag taaagtctga
    19981 agcaaatcgc ttttggttac gcctaataga ttaccaaata aaatatgggt taaatgctca
    20041 ccggtattaa aggcggtaaa cagcaagatc ccaaaggcaa acatacctga aaacacaatc
    20101 cccattaccg tgtcttcttt caagcgacta ttttctttta aatagcccac cgataaggca
    20161 cagcctaatc ctgaaaaaaa cgcccctatt gagagtggta acccggcaag tgaggaaatc
    20221 accacaccgg gcagaacagc gtgcgaaatt gcatcgccca ttaaagacca gcctttcagc
    20281 accagatagc aagataaaac cgcactcacg ccagcaacta aaattgcggt aattaacgca
    20341 atttgcataa attcaaattg caacggctct aaaatccaat taagcatttt gaacctccac
    20401 acggtgcaat ttccgctttt gagtaatcaa gccatattta ggagcaaagc agaatgcgaa
    20461 taagaaaatt aaggtttgta agcacacaat taccgcaccg gttgcaccgt ctaaaaagta
    20521 gcttaaatat gccccaatcc ctgatgtcag cgtgccgata agcattgcga tcagcactaa
    20581 tctagggaaa cggtcggtaa gtaaataagc agttgctccc ggcgtgacga ccatcgcaat
    20641 aaccaaaatt gccccaaccg tttgcagagc cactactaca caggcactta ataaggtaaa
    20701 gaaaatcact ttatagcgta atggcgaaag cccgactgac aatgcgtggt gttcgtcaaa
    20761 aaagacgagt agcaaatcct tccaaaatag gattaaaccc agtaacgaaa tcgcaataat
    20821 cagtaccacc tgccacaagt catcatcagc aatgcctaaa atatttccca ttacaatcga
    20881 attaacatca accgatgttg gattgaggga aacaataaac ataccaagcg caaaaaaagt
    20941 cgtgaaaata aagccgataa ccgcatcttc tttaattttc gtcaggcttt ttacccaaag
    21001 aatcgataat gccgccaaaa tcccggaaaa aaacgcacca aaggcgtagg gcaattttag
    21061 tgcataagcg atggcaacac ctggcactac agcgtgtgat aaggcatcac caatcaaaga
    21121 ccagcctttt aacatcaaaa aggcggaaag aaaagcacaa actaacccca cgcccatact
    21181 gacaaaaatc gctttttgca tatagttgta ggtaaaaggc tctaataaaa gatcaagcat
    21241 aaaatcctaa caagttgttg ataatagttc gcatatacta tcatataaac tccttttctt
    21301 gtatggatta aacttaaata tctactttaa attgataagg atcaggatag taataacgaa
    21361 aaccaagctc ttggcaaatt ttgtctgctt ttatcagcct tacaagcggt tgattttctt
    21421 cagaaaaatg caaatccgct aacccaaaac gttttgccat ttcactataa taatctttac
    21481 ggcttgggtg ctgttcggca cataaatgaa aaatacgctg cccgttcggc tgctccaaca
    21541 gcaatgaaat ggctgcaata cagtcctcta aatgcactaa attgaccggc tgccctgctc
    21601 cactcaagtt ctgttttccc gccaaataaa aaaccgggtg ccgttttttg cccaccaagc
    21661 cagctaaacg caaaatatca caatgaattg gctggctcag caaccaattt tccattttta
    21721 ctaataggtt agcagaatca accactgcat tttcatcaaa aacgcccgat ttcattggca
    21781 aaaccgaagt cgagctggtg aaaaccagat gtttcaagcc cttttctatt gcaagcgaaa
    21841 ctaatgtttg aataccgcaa ccgtaatttt cagggcgaat ttggctaagg ggcaaggcaa
    21901 tcactaacgc atcagcagca agtaattgct cgatttcgct tgtcactgct aacgcattta
    21961 aatctagagg ataacattct atcgctaatt ctccgaccgt tcgcttagta cctgcaacac
    22021 gccagccctt attttgtaat gcttcagcta aaggtagtcc gagccagcca agccctaaaa
    22081 tggtaatttt tttcatcatt tacgtttctc aagattaaaa ttggaatgag gggaaattca
    22141 ggtacaaaaa aagcacctcg tggtgctatc tattttattt ctagattgga gcgggaaacg
    22201 aggctcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg agctattccc
    22261 gcaaatttta aattgttgat tttctaaatg gtgcccgagg ccagacttga actggcacgc
    22321 cccgaaaggc gagggatttt aaatcccttg cgtctaccga ttccgccact cgggcagcac
    22381 gatatagaaa aattggagcg ggaaacgagg ctcgaactcg cgaccccgac cttggcaagg
    22441 tcgtgctcta ccaactgagc tattcccgca tttaaaaacg ctacgaacct cgtagagtct
    22501 caacaggtgc atattctatg gatttcagat ttaatgtcaa ataaatttgt aggaaatctg
    22561 taaaaaatcg tttaattggt gaaaaataac gctttttgca aatttttcat caaatttgac
    22621 cgcttgcagc cgccttattt gttgaaatca taatcagcaa atttccgaca aatatcaatt
    22681 acaacttttg tggctttttc catcgtatca accgaaataa actcaaatcg tccgtggaaa
    22741 ttttcgccgc cggtaaataa attcgggcaa ggcaagccca taaatgaaag gcgtgagcca
    22801 tcagtaccgc cacggatcgg ttctaaattc ggctcaatgc cttgttcacg cattgcgttt
    22861 tcggcaattt caaccaagta gccgtaatcg tccagcactt cacgcatatt gcgatattgc
    22921 tcgacaatct cgacttttcc gcaatgttcg ccgtaaatct gttgcatctc tgccagcgta
    22981 tcattcagca ttttgccgaa tgcagctaat tcatcacgct caaagctacg taaaatatag
    23041 tgaatttccg ctttatcaat tccaccctca atgccgtgca acataataaa gccttcacgc
    23101 ccttcgctgg tggcaggggt taaatgggga ggcagacggt gttggaactc gcaagctcgc
    23161 tctaaggcgt tgatcatctt gccctttgcc gtgccggggt gaacgctacg cccgaagaac
    23221 gtaacggtcg ctgcatcggc attgaaattt tcatattgca attcgcccag aggcccgcca
    23281 tcaaaggtaa aggcgacatc ggcaccgaat gcgtttacat caaaatgatc tgcgcctcga
    23341 ccgatctctt catcggtggt aaagccgatg cggatcttgc cgtgcggaat atgcggattg
    23401 gcttgtaaat actcgatagc cgagagaata atcgcaatgc cggatttgtc gtctgcgcca
    23461 agcaaagagg tgccgtcagt ggtgactaag gtgtcgccaa tcagcttgtt taaattcggg
    23521 aaatccgttg gcgaaagata atcgccactg ccgtttagca aaatattgcc gccttgataa
    23581 gcctcaatca cctgtggttt cacgttttca ccattaaaat cgggcgaagt atcaacgtgg
    23641 gcaatcagcc caagcaccgg cagggttttg tcaatattgg caggcaagct ggcgaacaga
    23701 tagccgttgg ggtcaagata aatctcgctt agccccatcg cctctacttc cgttttcagc
    23761 agatgaagca aatcccactg ctgttgcgag cttggcacag tttggctttt cggatctgaa
    23821 gttgtgtgaa tttgggtgta acgtagtaaa cggttaagaa gggattgttt cattgttttt
    23881 ccttactata gtttagtgta accaattctc caatttcaga tcaggtacca ttttaaatgc
    23941 ctgatcacta gaaactaaaa tacactcttc agcaagagcg tgactagcaa tcatttgatc
    24001 ctgcacgccc attactttgc catttttctc catctctgcc cgtaactttc cataaagttc
    24061 agcacaacga taattccaat ctaaaacctt tacactcgat aaaaaataat ctaaaaattg
    24121 agaaagctgc ccattatttc ttttcgccac accataaacc aattcagcag cagttacaga
    24181 tgaaatacaa agcctctcag gattaatgga cttcaatttc gccaccacat ttgcatttcc
    24241 acgaaaaaaa tagcttacag tattcgtatc taacatatac atttaagcta cctcaaaagg
    24301 atcccgttgc tcaaaggatt ggtttctctc ttttgtatct aaaaatgttt catcacatac
    24361 aacagcaggc aatagctcaa acaatcgttg ccaatgctcg gtatttctcg atctcaatga
    24421 taataaaata tcgccatttt cttttttaga aacatagact tgctctgcat caaactcaaa
    24481 ttcaaccggt aaacgcactg cttggttccg cccgttacgg aataatctcg caattttttc
    24541 catcttactt taccctccga tagatagtgt atatgccaaa gtatatgcct attttataca
    24601 actggcaaga taacccattc tcaaatactc aataacatac tgatttcaaa tcataatatt
    24661 accaactaat agtcggttta cgtttctgct caacgcaatc tttcaaataa agattgatca
    24721 acgtttggta aggcaaattc acctcttcgg caagttgttt aaaatagctg ataacatcgt
    24781 cttcaatccg aatagtaacc acacttttgc tttttagccc ttttgcgtaa gggtttttca
    24841 ctgcattaga aaaatcgtat tcatctctca tttaatgtac tcctttatat tgcccacttt
    24901 cgcgttttgt tgccaaacgt gctgaaataa tccgaatgtt ggtatttctc tcgcaatgca
    24961 caaccacaag tagattatct ttattgctac gcccgagtag caaaaaacgc tcttccctag
    25021 atgagtgatc tggatccggg attaacatcg cattattatc ataaaatacg cttaccgcct
    25081 cgataaaact aactccgtgt ttttgttgat tacgaagagc tttctcacta tcccattcaa
    25141 aaccattaat ttcttccatt gccagtttac tcctttttaa ttgtaaataa aatatattta
    25201 catctatttt tttaagttat aaacagccta gatgaaatta ctggattcgc aataaacaaa
    25261 aacgattttt acgatacaga tcgcaaaaat cgtttttcaa gattgacaac cttaggaggc
    25321 tttatccgcc cacaacctcc cttagcaaat tttccacaaa ttctaaccgc ttgcggttgt
    25381 cttcgagtgc taagcggaat ttgaacttca ccgccccttc aaaactgtac accgctggtt
    25441 gtttttggat taaggcgata aacttcattg ggtcgggctg ggctgtcggt ttaaattcaa
    25501 gataaccgcc gtgaatgccg gcatcgattt tcttaatccc gagcgtttgt gccgacaagc
    25561 gaagttgggt tatcgcaaac aggtttttgg ttgcctctgg gagcaagccg aagcgatcga
    25621 tcagctccac tttcaagtcg ttcaggcttt gcagattttc cgagcttgcg atccgcttgt
    25681 agaacgacag acgcatattc acgtcaggca gataatcgtc cggcaataag gccggtacac
    25741 gcaattcaat ctccgcttgg tgttgggtga tttcatcgag gctcggttct cgcccttctt
    25801 gcaaagcttt gaccgcattt tcgagcaagt ccatatagag cgaaaaaccg atgctttcaa
    25861 tctgtccgct ctgctccgaa ccgagcaatt cgcctgcacc acgaatttct aaatcgtggg
    25921 tcgccaacgc aaaaccggcc ccgagattat caatggtact catcgcctcc aagcgttgct
    25981 cggcatcttt ggtgagcgtt ttcgggtgcg gtgtgagcat ataggcgtag gcttggtggt
    26041 gcgagcgtcc gacccgtcca cgcagttggt gcagttgtgc cagcccgaat ttgtccgccc
    26101 gttcgataat aatggtattg gcagtcggca cgtcaatccc cgtttcaata atggtggagc
    26161 aaaccagcag gttaaagcgt tgatgataga aatcgctcat cacccgctcc aattcacgct
    26221 cacgcatctg cccgtgtccg atcacaattc ttgcctctgg cacgagttca gccaatttct
    26281 cggcacaatt ttcaatggtt gccacatcgt tatggaggta gtaaacctgc ccaccacgca
    26341 gaatttcacg caaaattgcc tcacggatta ccatttcatc actttgtcgc acaaaggttt
    26401 tgattaaaag tcggcgagcc ggcgggctgg cgataatcga gagatcacgc attccgttga
    26461 gtgccatatt cagcgttctt ggaattggcg tggcggtgag ggtcagaata tccacattgg
    26521 cacgcagttg tttgattttc tctttttggc gtacgccgaa acggtgttct tcatcaatca
    26581 ctaaaagccc aagatcacgg aacgctacat cgtcttgcaa cagtttgtgc gtgccgacca
    26641 aaatatccac tttgccctcc gccacatttt gcaaaatcgt tttctgctct ttggcggttt
    26701 taaagcgaga cagcacttcc acattcacag ggtaattggc gaaacggtct ttgaaattct
    26761 cataatgctg ttgggcgagc aaggtagttg gggcaagaat aaccacctgt ttatggttca
    26821 tcactgccaa gaaagtcgct cgaatggcca cttcggtttt gccgaagccg acatcgccac
    26881 acaccaatct atccatcgct ttggcttggc acatatcgct aattacggca ttgatggcaa
    26941 ttttctgatc gtccgtttcc tcataaggga aagtggcact aaactgatca aattccgctt
    27001 tatcgtaggc aaacgcaaaa ccggtttggc tttctcgttt ggcataaaca tcaagcaatt
    27061 ctgccgccac atcacggatt ttttccgctg ctttttggcg ggttttcgcc cacgcttcac
    27121 tgcctaattt atgtagcggc gcagtttcat ccgccccacc gatataacgg ctgattaaat
    27181 ggagcgaggc aaccggcaca tagagtttgg catcgttagc ataatgtagc acgagatatt
    27241 cggctttgat cccaccagcg tccagcacgg ttaagccacc gtaacgcccc acgccgtttt
    27301 ccaaatgcac caccgcctgt ccgattttga gttctgctaa attacggatt aaggtatcag
    27361 gattgacggc tttgcggttt ttctcacgcc gtgtttgcac acgttcgccc agcaggtcgg
    27421 tttcgcagat gatggcaagg ttgcgggcgg gcacaggacc cgcccctacg tagttattat
    27481 cattttcgcc atttgtattt aattcatcat cgtagggacg ggtcccgtgc ccgcccgttt
    27541 ccaccacaaa cccttgatca agcggtgaaa taatcaggga aaatggcaaa ccttgtgctt
    27601 catctaatga attgacctgc ttcggcttca ccccaagcgg agcaagcaaa tccagcaagg
    27661 tttctcttcg cccttcgctc tccaccgaaa acagaatgtt gccgtcaaat ttttggcgga
    27721 attgctggaa agcagaaaac ggctctttgg cagaggattg aatagcaata tccggcaagt
    27781 tagccacatt tgcattcatt ttcgctgctg atttacggat tttctccgaa gttatcgtta
    27841 aacggggata gcctttcagc cagcgattta cctcatcaat cgcaaaccag agatcactcg
    27901 gcggcagtag cggtcgcatc ggatccactc ggtggctttc atagcggttt tgggtatctt
    27961 ggtgaaattg ttcagctttt tgctgaatat cggcgaaagt gataaacagc gtttgggcag
    28021 ggaaatagtc aaacagactt gccatttcct caaaaaacaa cggctgccaa tactcaatcc
    28081 ctgcatttaa aatgcctttg ctgacttgct gataaatatg ctccggctca cggcggattt
    28141 caccgaattt ttcacggaat ttggctctaa aatgctcgat cccgttgcta tcagtcggga
    28201 actcgtgtgc cggcagtaaa ttaatttcgg caatttccgt tttggtgcgt tggttatcca
    28261 catcaaaagt gcggatactg tcgatctcat catcgaaaaa atcgaggcga aacggctctt
    28321 ctgctcccat cgggaatagg tctaaaatcg agccacgcac ggcgtattca ccatattcca
    28381 acacctgttc caccgcccga tagccggcat tttccagttg taaccgcagt ttttgaatcg
    28441 aaaaacggtc gcctttttta atcaacagca cattgttagc gagataattc ggcgggcaga
    28501 ccttctgcat taaggtgttg atcggcaaca gcaaaatctg ctgtcgccct tgttgcagtt
    28561 caaacagggc agaaagtcgg gcggagataa tgtcttggtg cggcgaaaaa ttgtcataag
    28621 gcaaggtttc ccaatcgggg aacagttgca ctggcagccc tgcaaactga ggcaaggctt
    28681 tttccaagcg taacgccgtg cgggtgtcgg gtgtgacaac caccgtcaag ccgtaaaagg
    28741 ctcgggcagc ttggctgatc gcaagggtat cggaatgccc gacaaggtta ccgagggttt
    28801 gatgatcttg aaaattgccg tcagatttcg gcagattaag tgaaattaaa gacatacaag
    28861 cggtctgttt taaataaaaa attgcaaatt gttgcttagt ttactaaagc acggctgatt
    28921 gtgcaaaata agcggagaaa ttatgttatc tagatcgcat tgcgaaaaaa ttctgataaa
    28981 atgttcccct tttttaactc ttactcacac atattatgaa aaataaaatt ttaggaagtg
    29041 cattaatgat tgcaggcact accattggtg caggaatgct tgcaatgcca ctcacctctg
    29101 caggaatggg ttttggggca accgcattac tcttagtcgg attatgggct ttattggcct
    29161 acaccggttt attatttatg gaagtttatc aaaccgctcc gcaaaaagat gttggagtgg
    29221 caagccttgc cgagcaatac tttggcttaa tcgggcgtgt attagccact ttcagtttgc
    29281 tcgtactact ttatgcccta cttgcggctt atattaccgg tggtggttca ctgttagccg
    29341 gtgtaatgcc tgaaatggca gatgctgata tgaaactgaa aatcagcatt ttaatcttca
    29401 ctgcggtatt aggtacattt gtggtagtcg gcgtgaaatc ggttgatggt ttaacccgag
    29461 tactgttttt gggaaaaatt gttgctttta ttgccgtgtt gctgatgatg ctgcctaaag
    29521 cgaagctgga aaacttgaca gcaattccat tagataactt actcattatt tctgctatac
    29581 ccattttctt cactgcgttc ggtttccacg ttattatggg aacaattaat agctatttag
    29641 acgcagatat cggcaaaatt cgtaaatcta tttatatcgg gacggcaatt ccgctagtgg
    29701 cttatttatt gtggcagctc gctacacacg gggtattaag ccaaaatgaa ttcaccacaa
    29761 ttttgacaca agatccgacc ttaaacggct tggtgaaagc cactagccag attaccggta
    29821 gtacgatttt aggcgaaatg gttcgcttat tctcagtgct tgcattgatt acctcatttt
    29881 taggggtttc gatgggaatt tttgaaggcg tgggcgattt gttaaaacgc ttaaatctac
    29941 cgactaaccg tttatggctg accatcgcca cctttacacc gccaattctg ttcgctttat
    30001 tctatccgaa cggctttatt aaggcattag gttacgccgg tttactcttt gcgttctatg
    30061 gtatgatttt acctatcggc ttagcgtgga aagcacgtcg ccaatatccg aatttacctt
    30121 accgtgtgat tggtggtaat ttggcattag tggttgcgtt aattgccggt attatcatta
    30181 tggtcattcc gtttttaatt caggctggct atttaccgca agcggtaggc taatattgaa
    30241 aagggctacg attcaatatc gtagcccttt ctataagtta caagcggtca tttttcgatg
    30301 actttttaca gattagccaa tcaactgata cagcgcctta atttgatgct cttttaatgc
    30361 cccgccaatt ttctctctgc cgacaaaaat cttaaacatt gcgttgcctt ttttattaat
    30421 gaaaatcagt gaaacagtac gttttcccat aaaaggacga tccaacaaat aaattttggc
    30481 acaattttcg tagcgtagat gcccttgtaa gcctccgccc tctttatgtt cgaaattgta
    30541 aaatccacga gaaattgaac cgcttgggag ctttcccaca aactcaataa tggtatcttc
    30601 cgtatgcgaa atcagcgtaa tcgcatcgtc ccattcgtgg atttgctgta gcacttcggc
    30661 aaaacgttcc ccttcggtta cttttagcat ttcagccggt aaattcagca ttacctcttc
    30721 cagcgtgcat tgatattttg ccgctaacat ttctaaaatt tgccccggat tttcggctaa
    30781 ttcattgcgt aaggcggttt gttgttcgat tgatatcatt aaatgttcct ttaagtaaag
    30841 agagtttctt agctctctaa gtaaatttgt aaattagaaa cggtattcaa aattagccga
    30901 gaagttacgt ccaggttcat aatagcgttg taatccagga tagccaccgt aagttgcatt
    30961 ttcacgtact ccaacagaat taacgattcc acctgttgta tttaaaccac gtaaagtatc
    31021 ccaagtatgg tatttacggt taaacaaatt ataaataccg gcacgtaatg taatattctc
    31081 agttggttta tagaaaccgt ataaatccac aacaaaggct ttagcattta agtgcttcca
    31141 ggtatctaaa cctgtctcgt gagaatattc agtacaacga atgtcatatt catcccccca
    31201 ataagggtaa tacggatttg gcgttctatc ctctttaagg catttttccg gtaatgtttt
    31261 taaatatttt gcatctttcg cctttttagc ccccaagaat gtgaagcgag aaaatactcc
    31321 ccatttacct tcaggttgtt cataatctaa gccaactacg gctttaatcg gctgaataga
    31381 aagtaaatct gccccgttag acatactacc tttgctgtaa ccgaaggctc caaatagctt
    31441 ccagccttca ggtattggca tagctgaatt taaattccat ttaccactca cttctagccc
    31501 gtagattttc gcagaatcaa tattttgcat ttgctgtacc gggcgccaaa actctctacc
    31561 atatgcttgg taaacatccc aggtttcttg ctcccataaa aaatctttat atttagtatg
    31621 gtggaaatta actgagagca gcccaacatt gccactacct tgcaaactta aattttggct
    31681 taaactacgt tccgctttta ggtttggatt ggacatccaa gctcctgcag cattatcacg
    31741 gtaatcaaaa tacatttctg atgcatttgg aatacggaag cctgtcccaa tgttatatcc
    31801 tactttccat gcctcagtca gatgtttttc taaaccggta acccaagtta gttgattaaa
    31861 tttcgcattg tcagttttta cgcagttacg acaaacaaga ccattgagtt cttgttgttc
    31921 atacttcgcc caatcataac gaatgcctag cgttgcaatc caatcatgcg ttaaatcaat
    31981 cttatcgtgc aatgaaaaag tgtgctgttt cgttttaatt gggtacatca tcgtagaatc
    32041 gggagaattt acccaaacac cggataaata aaccgagtcc ttatgtaata catcgaaatc
    32101 tctctctgca gtagaggctt tgaaagatag ctgatgagtc catttaccaa attctaatgc
    32161 ttggctgtct aaacggaaat ttaagcgttt caattcagta ttaaaaatac gaatattatt
    32221 gtcacttggt tttctttgtt tagcagggct ccagtctgtt gcatcttcag cgcgtgtacc
    32281 ttcatagtta taagcagatg tttcagtatc ttgataatct gcatctatct tcgctagaga
    32341 tatccatttt gactctggga aatactcata aaaaacattt actgtatcac gtttactacg
    32401 gtcttccgct tcacgccaaa aagaagtaat agtaacggct gaatcttcga taatataatt
    32461 tttattattt tgcccactgt aagaaacacc aacgcggtga ttatcattaa atcgataagc
    32521 aaatttggct aaataattat gatgattatg agtagaatca tcaggagttt gtttactact
    32581 gcgtaaagag cgagtttgta gagaatcttc aggaagtgta taacctcctg cacttttcat
    32641 ttcatgccca tggcgatttg aataaaccaa tatcgcctca gctttctcac ctaaatatcc
    32701 aacaccgaat gtattcaacc attcattatt acgagtacca tatcctgtgc gataaaaagc
    32761 accaaattta ttaccgttac gaacaatatc ataaggctca agcgtccgat aattaactcc
    32821 gccgcctaaa ttaccactac cttgattgaa agaatctgca cctttagata cttcaatagt
    32881 acgagctaat tccggatcta tagattggcg agatgtattt aaattaccat agcgtttata
    32941 aagggaattt tcttctgaat caggaaaagg cgagacacca tcaatactta ttcctacacg
    33001 gttgtcttcc actccacgaa tagcaaatcc tttttgatgt ctaccttggt taacaatgcc
    33061 aacatcaggg ctataacgaa ctaaatctcg attattttga atcaactctt gctgaattat
    33121 ctctttattt ttcttttcta tagaagcctt cacatcttct ttagcaacaa catttacttc
    33181 agataattcc gctgttgttt cttccgcata ggtagcttgt gaagacaaaa tgctactaac
    33241 gcttaaagcc aatatagtcg ggcacaattt tttagacttt attgaataag aggtaaac
//
LOCUS       DANLXQ010000008        32286 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.008, whole genome shotgun sequence.
ACCESSION   DANLXQ010000008 DANLXQ010000000
VERSION     DANLXQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32286
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.008"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(397..2514)
                     /gene="spoT"
                     /locus_tag="PXD94_000304"
     CDS             complement(397..2514)
                     /gene="spoT"
                     /locus_tag="PXD94_000304"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL6285208.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQNSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIRQSLKNQQHDEAIALGRRQLVRAMNLRSLDELCSETI
                     QSVLDELRLSSFDELLAEIGLGNQMSNVIAQRLLGEPLLIDTDGNPENNQPLKIEGIE
                     NRLISFANCCLPIPGDEIIAHVSSGKGLAIHNACCGNVKDYATEPSNYLPVEWEIAKE
                     KAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINSEAREGNIYQVRVQISVENK
                     EHSELILRKLLSVKGVVKAARKQ"
     gene            complement(2624..2905)
                     /gene="rpoZ"
                     /locus_tag="PXD94_000305"
     CDS             complement(2624..2905)
                     /gene="rpoZ"
                     /locus_tag="PXD94_000305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL6285209.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KPTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(3008..3505)
                     /locus_tag="PXD94_000306"
     CDS             complement(3008..3505)
                     /locus_tag="PXD94_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL6285210.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(3530..4552)
                     /locus_tag="PXD94_000307"
     CDS             complement(3530..4552)
                     /locus_tag="PXD94_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6285211.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(4592..6022)
                     /locus_tag="PXD94_000308"
     CDS             complement(4592..6022)
                     /locus_tag="PXD94_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL6285212.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVENSDRILFGWVGLPDLTYPTDKYQWHSMLTLPRKLSLQNGKVI
                     QNPIVNVGELQAVEMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENG
                     VLSLDRSQTEQTELMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFI
                     AKRENVVKSSRPIQVKVAKVLPISVE"
     gene            complement(6127..7050)
                     /locus_tag="PXD94_000309"
     CDS             complement(6127..7050)
                     /locus_tag="PXD94_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL6285213.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            complement(7167..8594)
                     /gene="miaB"
                     /locus_tag="PXD94_000310"
     CDS             complement(7167..8594)
                     /gene="miaB"
                     /locus_tag="PXD94_000310"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL6285214.1"
                     /translation="MAKLHITTWGCQMNEYDSSKMADLLNSTHGFELTENPEDADVLL
                     LNTCSIREKAQEKVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVF
                     GPQTLHRLPEMINQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYC
                     SFCVVPYTRGEEVSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFA
                     ELLRLVAAIDGIDRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMM
                     KRNHTALEYKAIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFS
                     FIYSARPGTPAADLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPS
                     KKDLMELTGRTETNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVV
                     ESAESVIARTRKEDDLGVGKYVVNL"
     gene            complement(8811..9311)
                     /gene="crr"
                     /locus_tag="PXD94_000311"
     CDS             complement(8811..9311)
                     /gene="crr"
                     /locus_tag="PXD94_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL6285215.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPSGDTIVAPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGEPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
     gene            complement(9396..11117)
                     /gene="ptsI"
                     /locus_tag="PXD94_000312"
     CDS             complement(9396..11117)
                     /gene="ptsI"
                     /locus_tag="PXD94_000312"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL6285216.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDVGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            complement(11239..11496)
                     /gene="ptsH"
                     /locus_tag="PXD94_000313"
     CDS             complement(11239..11496)
                     /gene="ptsH"
                     /locus_tag="PXD94_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDL6285217.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISADGEDEQKAVDFLVDLIPTLE"
     gene            11757..13331
                     /gene="thiP"
                     /locus_tag="PXD94_000314"
     CDS             11757..13331
                     /gene="thiP"
                     /locus_tag="PXD94_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease"
                     /protein_id="HDL6285218.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIRVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYTKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            13328..13978
                     /gene="thiQ"
                     /locus_tag="PXD94_000315"
     CDS             13328..13978
                     /gene="thiQ"
                     /locus_tag="PXD94_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL6285219.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            14285..15699
                     /locus_tag="PXD94_000316"
                     /pseudo
     CDS             14285..15699
                     /locus_tag="PXD94_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
     gene            complement(15815..16618)
                     /gene="map"
                     /locus_tag="PXD94_000317"
     CDS             complement(15815..16618)
                     /gene="map"
                     /locus_tag="PXD94_000317"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL6285220.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
     gene            complement(16704..17369)
                     /locus_tag="PXD94_000318"
     CDS             complement(16704..17369)
                     /locus_tag="PXD94_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL6285221.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAIAFYKSSDEYMAEHWFTIGSVDYAFKLFVSLLLFLPLYGVILNFIMKKL
                     DLK"
     gene            17562..18251
                     /locus_tag="PXD94_000319"
     CDS             17562..18251
                     /locus_tag="PXD94_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL6285222.1"
                     /translation="MLQLRKANERGDGSFSWLKSYHTFSFANYYDLKHIHFSHLRVIN
                     EDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDSMGNKTEVKAGEFQIMSAGTGVFHS
                     EINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFAPKEGATLILSPNAEEGSFKIYQDM
                     KLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNINGVELSTSDALGIRQEKALEINTQ
                     DDVEFLLFDLV"
     gene            complement(18319..19110)
                     /locus_tag="PXD94_000320"
     CDS             complement(18319..19110)
                     /locus_tag="PXD94_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL6285223.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQEIEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETV
                     LWNPWHNTVSAMTETAYQNMVCVETARINTLLQQNETLGVRILVR"
     gene            19234..20238
                     /gene="fbp"
                     /locus_tag="PXD94_000321"
     CDS             19234..20238
                     /gene="fbp"
                     /locus_tag="PXD94_000321"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL6285224.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
     gene            20422..24933
                     /locus_tag="PXD94_000322"
     CDS             20422..24933
                     /locus_tag="PXD94_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6285225.1"
                     /translation="MINKNFPLSYLASFVAFAVSLSATASVFRDDIDLQYYRDFAENK
                     GKFTVGASNIEIKDKSGNTLDALFPNVPMPDFSAANRNLGIATLVAPQYLVSVAHNTQ
                     YNTVEFGAPGTNADAHHYTYKVVDRNDYGIVEGGQHQDYQVPRLNKLVTEVAPATVTD
                     LGNNASAYQDSSRFTHFARLGSGRQIVKNTEHKDNQISTSYQYLTGGVHLPIAVHNSD
                     YWLDFRGNALNNSPYGALTAFGTRGDSGSGVYGYDQKTKRWLLLATYTFGTPANNYYN
                     RAGIIRQDYHDKQFAEDIAGTLTNAQQNAVFEWSAQGKDSSIGNKGKNLTVSLADRSI
                     KDNSASPLGYGGTPQLQLPQDNTGKTLNIEGQDSTIVLKTDIDQGAGALNFNANATVR
                     PENDQSWVGAGIVVAKGKQVNWQVKNPQGDRLSKLGEGTLHINGKGENLGDISVGQGT
                     VILTQQAGENGKKSAFNQVGIVSGRSTVVLNSADQVDPNKIYFGFRGGRLDLNGNNIA
                     FNRIQNSDDGARIVNNHLQKAATLTINGPKPPEATDLKWGTWKENSADIYEYINPHAN
                     NRTDYFTLKGNPNQYMPTNGASNAHWTFLSSNKDAAVKQVLAQKGLEHRYNSFNGFIG
                     ETDNTQHNGRLNVVYDPKASTPPATASEVTWGKGLVAGADIYLFTNPKTKIREYFALK
                     GDPKQPVPRGGLSSEHWEFLAADRNQAINKVLARKNAPIEQEKLNSIYTFSGGLNLNG
                     DLTVKGGKVLLSGRPTPHAYDAINKQDVVYADDWQNRQFKADNMQLNQYAQLYVGRNV
                     SNLQANLSANDHAQLHLGFINEQTPSCYYSEYTGKTSCDTQAVVSNEIFATLPTTQIN
                     GDVSLAEQSQLHIGKANLIGTIQAAATTSIRLANQASWTNTGDSRTGNLVAENGSTIN
                     LNEKFVTGEIPTRFNTLIIDGNFQGNAKINYLTDIAAGKGDHLQVNGLAEGTFTLALR
                     NSGKEAEVVSPLSLLTLTHQAQADKAKVSLENGYVDLGAYRYVLANRSNGYRLYNPLK
                     DAKDRNQSIATAKAELDHAIAEADKQKQEISRLNAEAEKERQAEKNAKQAAANAQSQL
                     SQANSELTRLQQYADYYRRYYPTYYRQIQGQITVAKQKVTQASAALTTAENNAKASAA
                     QIANVEQAVETAQNIAKQVEAKLATLRITAGNSEAILNAEALKLCQENGANCEHLQAH
                     ADTDSSNIQQSDWVSQYANTALSELSAQANSALQIGQDLDRQLFAKHDKFHVWSSIEH
                     QKTEHQSDLYRPYEQQTNLTQLGIELPLANGINAGVMLSRNHANAEFDEGVNGKSNLL
                     MASLYGKWHSENGTFVSLDGSYGKAKNRIDLFGENRFNRHIMAIGANLGHNFDLAGVQ
                     VQPAVGTRYYRFSAQNYKLGEVEVRSPKAHFMAYQAGVKVSKAFDLSSWKVEPSLAAH
                     YVDASSKRLSVAVNDNTFSQRFGRYLKTEVGVGVTKGQWQLSSHLGLLKGNEIGKQHF
                     AGFKLGYSW"
     gene            complement(24988..26097)
                     /gene="mobA"
                     /locus_tag="PXD94_000323"
     CDS             complement(24988..26097)
                     /gene="mobA"
                     /locus_tag="PXD94_000323"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL6285226.1"
                     /translation="MEEKISAVILAGGLARRMNGAEKGLQLLDQKPLISHLLARLSPQ
                     IEDIWLNINRSVEQYQQLYPELPYYQDSLPDFQGPLSGMLSGFEQIESDYLLFVPCDT
                     PFIPTQLLHKLHTALRINQAQIAYAHDGDRPHPTVALVHRSVAENLRAYLASGERRLL
                     WFFQTQKSVAVDFSAYKAAFRNFNCLEDLTAFNPYPIKTLAITGYSGTGKTTLLEKLI
                     PKLTACGIRVGLIKHSHHNVDIDKKGKDSYRLREAGANPTMVVCDERWALMTETKQAA
                     ETKQAVEFSQLIAKFDPETVDLVLVEGFKHETMPKIQLHRKEIEKPLPALDQWTIATA
                     TDYPLERENRLDINDVEQLADFIRQWLEASKEPAN"
     gene            26189..26458
                     /locus_tag="PXD94_000324"
     CDS             26189..26458
                     /locus_tag="PXD94_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDL6285227.1"
                     /translation="MKCKRLEELLELLGEHWRKNPDLHLIDILQQLSVEVSEPDNFHA
                     LRDEVLIYQLKMRNADKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            26502..27140
                     /locus_tag="PXD94_000325"
     CDS             26502..27140
                     /locus_tag="PXD94_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL6285228.1"
                     /translation="MKKFALKTVLVALSALFAFNATAVAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELNYKIPSQIKAKLPADSKLVQYHVNFLGRQSENLTRAWAL
                     AMALGAEDKVKTALFEAAQKDAFKSMDDIRAVFIANSISAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFSDSSSTNDFIQRYIDAVVFLSKK"
     gene            27160..27612
                     /locus_tag="PXD94_000326"
     CDS             27160..27612
                     /locus_tag="PXD94_000326"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HDL6285229.1"
                     /translation="MVLQVLNSKAIQPFLYANSENKMTNLSILNLVPIREGQTAKQAV
                     DSMVKLAKHAENIGIERYWIAEHHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLP
                     NHSPYIVAEQYGTLETLYPNRVELGLGRAPGTDMRTASALRPEFAFPR"
     gene            27596..28258
                     /locus_tag="PXD94_000327"
     CDS             27596..28258
                     /locus_tag="PXD94_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL6285230.1"
                     /translation="MPFPDEISELKGYFDGTNPVSAYPAQGLNVPFYILGSSPESAYL
                     AAERGLPYAFASHFAPRFMDDAIRIYRSHFKPSDVLDKPYVILGVNAIVAETDEEATQ
                     LATTQTQFFLNVVTNAQQNLQPPLESDDKVWENFVKAEIVPHFGPVAFEQNALYRQEK
                     TVVRQMTECSLIGSPQSVEQQLKALKARVEIDEIMAVNYIFDEQKQHQSYTWLKQITD
                     KV"
     gene            28260..28553
                     /locus_tag="PXD94_000328"
     CDS             28260..28553
                     /locus_tag="PXD94_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL6285231.1"
                     /translation="MSKTMQQKAAEAHNMCRLKGDSMLDNADRQIAFEAVYESEELAQ
                     QAVEFFAQKAKSVETEPCEIQSEITQIAEGFLMKMWITFSCQAEVILFQMAVR"
     gene            complement(28622..29956)
                     /gene="srmB"
                     /locus_tag="PXD94_000329"
     CDS             complement(28622..29956)
                     /gene="srmB"
                     /locus_tag="PXD94_000329"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL6285232.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRRKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
     gene            30116..30379
                     /locus_tag="PXD94_000330"
     CDS             30116..30379
                     /locus_tag="PXD94_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL6285233.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            complement(30427..30525)
                     /locus_tag="PXD94_000331"
     CDS             complement(30427..30525)
                     /locus_tag="PXD94_000331"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL6285234.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(30525..32033)
                     /locus_tag="PXD94_000332"
     CDS             complement(30525..32033)
                     /locus_tag="PXD94_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL6285235.1"
                     /translation="MSNQSSNAPQRETFSGRGAFIMAAIGSAVGLGNIWRFPYTTYEN
                     GGGAFILPYIIALLTAGLPLLFLDYSIGHKYRGGAPLAFRRFSSKFETFGWWQVLINV
                     IIGIYYAVVLGWAASYTYFSFSGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGP
                     LIAVWLIAIAVLAMGIKKGISAASNLLMPQLIVMFLVLVGYSLTLPGAAKGLDALFTP
                     NWEKLADPSVWIAAYGQIFFSLSICFGIMITYASYLKKETDLTGSGLVVGFANSGFEL
                     LAGIGVFAALGFMATAAGQEVNEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLT
                     FAALTSFISVIEVIISAVQDKLNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMD
                     KFVNQFGIVAVAFVSLIAIVTSEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFM
                     LFSEIAKVYREGYEGYPSWFVNTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
BASE COUNT         9526 a         7147 c         6275 g         9338 t
ORIGIN      
        1 agaaaatttt cgctaagccc accatcttga ttaggctttc tagatatatg ccaaaatcca
       61 gataaacgat aataccctac ctgagataat tttttctctg catagctttt atcatttatg
      121 agcattcctc tagatatgag tagttctact aattcgtgat actctttatg tggttttgta
      181 ggcataaaat tgaaggcatt ttaagaaagg caaggacgta gcttgacact ctaagtcgat
      241 tacatttagt aaacctcagg gacaggggct acgccacgat tgcaagggat tatacatttt
      301 agctgattaa agtcaaaagg tttcatcaat taataatagc aatactgtac ttttaaggac
      361 tataattgca aaaaactgat aaaattttac cgcttgttac tgcttacggg ccgcttttac
      421 aacacctttt acacttaata gcttacggag aatcagctca gaatgctctt tattttccac
      481 agaaatttgt actctcactt gatagatatt tccttctctt gcctcgctat taatgctgcc
      541 aatgttgcta tttaatttgg caagcgttga tgtgatcccc gcgagtaccc ctggttcatt
      601 gataatatcc agtacgattt ccgtttcaaa ttgaatcgct ttttctttgg caatttccca
      661 ttccacaggt aaataattac tcggctctgt tgcgtaatct ttcacattac cacaacaagc
      721 attgtggatt gccaatcctt tacctgaact gacgtgagca ataatttcat cacccggtat
      781 aggtaagcaa cagtttgcaa aactgataag gcggttctca atcccttcaa ttttaagcgg
      841 ttgattattt tctggattac catcagtatc aattaatagt ggctccccta ataagcgttg
      901 agcgataaca ttactcattt ggttacctag accaatttca gccagtagct catcaaagct
      961 tgataaacgt agctcgtcta atacggattg aatagtttct gaacacaatt catcaagtga
     1021 gcgtaaattc attgcccgaa ctagctgtct acgcccaagt gcaatcgcct catcgtgctg
     1081 ctgattttta agcgattgac gaattttagc cctcgcctta ccacttacta caaaatttaa
     1141 ccacgatgca tttgggcgct taccttgctc ggtaataatt tccaccgtct gtcctgttcg
     1201 tagcggttct gaaagtggat acggattgcg atcaaccatc gcacctacac aacgatcccc
     1261 aatgcctgaa tgcaccgcat aggcaaagtc aatagcggta gcattcgccg gcaattcaac
     1321 aatacgccct ttcggggtaa agacgaaaat atcgtcagag aacagatccg atttaacgct
     1381 atcaataaat tctgctgtat tgccggcact gttttgcaat tcaacaatac tttgtagcca
     1441 ttgctgggct ttgagttgaa cggtcgtgtt atggtgcgac tcgccctctt tgtaacgcca
     1501 gtgagctgtt acccccatat ccgccatttt gtccatatct tcggtgcgaa tctgtacctc
     1561 caccggtaca ccgtgcgggc caatcatcga agtgtgtaaa gattgatagc cgttcggttt
     1621 tggcacggca atataatctt tcacccgttt cggacgaggc ttatacagtg cgtgcatctg
     1681 ccctaataca cggtaacaat tatctacatt atcaaccact actcggaaag cataaatatc
     1741 taaaatcgaa tgaaaatgtt gatctttctg gcgcattttt tggtaaatgg aatagagatg
     1801 tttttcacgc ccatacacac gggcttcaat ttgcacctct tctaaccgac cacgaatatc
     1861 atcggcaatg cgttgaatta gctctttacg atgtcctctg gcactggcaa tagccatttt
     1921 cagtacccga taacgttgcg ggtgcattgc ctcgaagcct aaatcttcca actcgttttt
     1981 aatatgctct attcccaagc gatgtgccaa cgggctataa atctctaagg tttctttcgc
     2041 tatacggcgg cgtttatccg gacgcaaagc accaagcgta cgcatattat gggttcggtc
     2101 ggcaagttta attaacacaa cccgaatgtc tttggtcatt gccaaaatca ttttacggaa
     2161 gtttgccact tctgcctctt ggcgggtgcg gaattttaat ttatccagct tagaaacgcc
     2221 ttgtacaata tctgctacgc tctgtccaaa ttccgccgcc agttcttctt cggtgtaagg
     2281 tgtatcttca atcacatcgt gcaacaaagc cgcaatcacc gcttcttgat cgagcttcat
     2341 ttccgcaata atcgacgcca ctgctaccgg gtgagtaata tagggctcac cgctagagcg
     2401 tgtttgtccc tcgtgagcat cacgggctat cacataagca cgttttacac gctcaatctg
     2461 ctcgggtggc aaatagcctt gaataattcg atctaaaggt tcaaaaagat acacgttata
     2521 cctcaacaac agaaataaaa atggacaacg aatttgcaaa ttttgaggaa aatcctaccg
     2581 cttgcagact caattgtcca ttctctattt acgaaaaata aaattacgct tcgttgctca
     2641 tttcagttaa aaatgaaatc gcctcttgct ccgccgcttc ttgcttaata gagtcgtgat
     2701 tttccagctg atccataatg tcagttgtaa ttaaaccttc ttcaatttca cgtaatgcaa
     2761 tcacggtcgg cttatcgttt tcttcaggca caagtggctc acgggtgtga agttgtaatt
     2821 gtcttgcacg acgtgcagca attaagatta aatcaaaacg gttgccaatt ttctccgctg
     2881 cttcttgtac ggttacacga gccataaatt tactccaaag atttattcgg ttttattcat
     2941 aaaaagattg cgaattatac agaaattccg ccaattccgc aatgaaagcg ggctaaattc
     3001 acaaaattta tttggtattc agtaaaatcg ccaataattc agcctcaacc tctttgccga
     3061 attttcccat tctaaaatcg gcttttagtt gctcgaattg ctcagggttc gctgccattt
     3121 tatcttttaa aaattgcgga aattcagata acactaattc tgttttgcca ttacgaatat
     3181 caaaaatagt tggaattttt tgcgcagaaa tgaccgcttg ttgctgttca tcgctgtcaa
     3241 aatgtttgcg ttcctgatta tctttgcgag ccacatattt caccttaatg ttaggattga
     3301 gctttgccat tcgctggaaa taagccacaa tttccacaca atcggggcaa tatggctctg
     3361 ccattaccag ccagtcccct tgcacgccaa cagcttgcag tttttcttcc gtatctgtgg
     3421 aaagcttgac ctccacatac acttctaatt gattttcacg atcttcatcg ctgttaaatt
     3481 ctaaatattc gtaaaaatgt gccataattt tgtcttttcc tatctcatct taaatggatt
     3541 tacgccataa aatttcacaa tcaactttaa cgtcatctat tttctgttgc gctaactttt
     3601 gctgaattaa ttcaaaggta cgttcggcaa tttgttggtg gtcttgctta attgaatgaa
     3661 tatgaaaagg tagtgcattc agtaaatggt gatcatcaaa ggttgctaaa tgcagttctt
     3721 gagtcaataa tttatccatt tgttggtgtt cagtcagata gcgcattact ccctcaagta
     3781 gggtaaaaga ggctgtgaat accgcttctg ggagcctgcc aagtttttca actatctgag
     3841 cgaacatttg atagcccgat tccggctgat agtcttgatg cagaacccaa ttttcatcaa
     3901 gtgccaaatg tgactgctcc aaaccttcac ggaagccttt taaacgattt tgacttggcg
     3961 agagtgaaag ttgtccacct aagtaaaaaa actcttttaa gtgataagcc cgtacaatat
     4021 tttccaccaa catactgata ctttgtgtat cattactggt gatagaaggg atattcaacc
     4081 cagaaatata gcggtctagc tgtaacattg gggtatattg tgccgcattt tgataataag
     4141 tcggctcatt atgtgtcgga gcagtaatca gtaaatctac ctgtctgtct aataaacgct
     4201 ctatcgcttt cttctcacct tcaaaggtat catccgagca agcgatcact agctgtaacc
     4261 cgttttcacg gcataatttc tctaaggttc ttgaggtcag agcaaagcca taattcgtta
     4321 aatcgggaat gactaaaccg atcacattcg tgcgttgagc ctgtaatgat tttgcatata
     4381 cattagcacg ataaccgtgt tttttagcga tctctttcac tttttggcag gtttcggatt
     4441 tgattctaaa atcattgccc cgcccattta aaatcatact gactgttgtt ttagatacgc
     4501 cgctgagttc cgcaatatca ctgagcgtta ggcgtttttt agtcacactt atctcctttt
     4561 agcataaaaa tgggcattac tgcccattgc tttattctac gctaattggc aatacttttg
     4621 ctactttcac ttgaatcgga cgagagcttt ttacgacatt ttcacgctta gcgataaaaa
     4681 agcgtgaagt cagcactttt tccccgccgt ttacgaaaat ttccaccaca gaacggtcaa
     4741 agaaaatctc gattgtctct aattgttcaa ttttgcaatg acgaaccgag ccaaattttt
     4801 ccatcaattc tgtttgttcg gtttggctac gatctaagct caacacccca ttttcaaaac
     4861 gtaagctgag cttttgcccc tgttcattgc tgaaaaaatc caacacaagc ggttgatttt
     4921 ccacagaaat ttgcaagtag gcagtatcca aattctcgat tgcaaaattt tctgacattt
     4981 cgaccgcttg taattcgcct acattcacga ttgggttttg tatcactttg ccgttttgca
     5041 gggaaagttt acgaggcaat gtcagcattg aatgccactg atatttatca gttgggtaag
     5101 tgagatcagg caagccgacc cagccaaaca aaatgcgatc ggaattttcc accgtttgcg
     5161 gtgcgtaaaa gtcaaagccg tagtccagct cttcaatatg ctctgcccgt aatctgttgc
     5221 cgtttagctc gccaagtgcg taagtggcgt ggtagttatt ttggaattga tggctttcac
     5281 gcagtttgcc ttgtggcgac cacacaaaca gatctttacc accaagtgag aacagatccg
     5341 gacactccca cataaacaca ttgctgttat caaaattgtg gacttccagt tctgcgatta
     5401 aacggggtgt ggcatttaaa tcgtccattt cataaattaa cgccgtgccg gtcaagtttt
     5461 cacgctgagc ccccaacaca aaacgaattt tgccctctgc ggtaaggtaa ggtttcgggt
     5521 cacgcacgtg ttcggtgtag cctgccggtg cttggtcgat aatgcaacgc ttttccaata
     5581 atttgcccga tttatcaaaa atcgcgatat tttggtgagg aatgcgggca ttatctgatg
     5641 ggcggcgtgt attgccggtg taaaaagcga caatcttatc ttgccataaa atcgccccac
     5701 ctgaataaca gccgtgggat tcaaacagct catcgggaat cagcaaatcc cctttactga
     5761 acgtttgaaa atcggtagtg atgaaatgct gccaatgttt cattccatgt aatgccccat
     5821 aaggaaacca ttgggcgaaa atatggtatt tttcaccatc gaaaatcaag ccgtttgggt
     5881 cattaaataa gcctgtttcg ggagcgagat gataggttgg gtaaaaatcg ggatcagata
     5941 aaacggtttg gcgaagagcg tttaattcgc cctcgctttg tgcgtgcagg cttttgtatt
     6001 tgccgttgtt gaaaatgtgc atagagtgtt cctgtcattt cagaaattac gatggtgcaa
     6061 gcgtccacgc ttgtaccctt ttgtaaaaaa tgaggcacaa gcgtggacac ttgcgccatc
     6121 attacattat tccgccaaaa atgcgtctaa ctccgcttta ttcggtaatg ccgacatcgc
     6181 cccttttgct gtggtggcta acgcaccgga agcatttgct ttgcggatca cttcaactaa
     6241 cacagtttca tcgtgccaat tcggcgtttg agcaagaccg gctaataatc cgctgacaaa
     6301 ggcatctccg gcaccggtgg tatccacagg tttcaaggct ttgccggcaa cgacttggct
     6361 ttttccgttg aaatggtaga ttgcaccgtc tttgccaagg gtgataataa tcaatttctc
     6421 cggatactga gcggtaatag cttgtgtggc ttgttccaaa gtttctgtct cggttaataa
     6481 ggtcagctct tcttcggaaa atttgagaat atccgccatc gccaccacag aatttaccac
     6541 tgttttcatt tcatcaaggc tcttccagag cgattcacgc aagtttggat caaacgaaac
     6601 atagccaccc gcctttttca ctcggcgaat cccttcaatg gtagtggaac gggatgggtc
     6661 gttaatcaag gcaatagaac agcagtgtaa gaaatcacct tggttaaatt caggtaaatc
     6721 gccgatttct aaaaattggt cggcactcgg atttaccata aaagtaaaac tacgctcacc
     6781 gttatctaag ccaacaatca cggtggaagt gcggtgctga gcgtctaaaa tcatctgttt
     6841 ggtgcaaaca ttttctgcct gtaacacttc tcgcataaat ttacccagag ggtcgttacc
     6901 cacacgcccg ataaatcctg cctcaacccc taaacgagaa acgcctactg caacattcgc
     6961 cggagcacca ccggcacatt ttaaatagtg atgttcgccg tcgggaatta aatccaccac
     7021 cgcatcgcct gttacccaaa tcttactcat ctcatacacc tttaattagt cacaaaacac
     7081 caagctaaaa cgatttagca tttattcgat tatatactaa aagaccgctt gtacatacct
     7141 acaagcggtc attttttctt taatttttac agattaacca cgtatttacc tacacctaaa
     7201 tcatcttctt tacgagtgcg ggcaatcacg ctttctgccg attccaccac acgtaatccc
     7261 atttggtctt cggtacgaat cacttcgcca cggagtgagt tggtataaac atcggtgatt
     7321 ttcacatcga caaatttgcc aatcatatcc ggtgtgccaa caaagtttac tacacggtta
     7381 gtttcggttc tgccggttaa ttccatcaag tctttcttag acgggccttc aaccaaaatg
     7441 cgttgttctg taccgagcat tgcacggcta aattgcattg cttggtggtt gatgcgttgt
     7501 tgtaaacggg ataatcgctc ttttttctca tcatcggaga cattatcagg cagatctgcc
     7561 gctggtgtac ccggtcgagc cgagtagata aagctaaagc tcatatcaaa gttcacttgc
     7621 tcgatcactt tcattgtttg ctcgaaatcc tctgcggttt cgcccgggaa gcctacaata
     7681 aagtccgaac taatttgaat ctcaggtcga acagcacgca atttacggat aatcgctttg
     7741 tattccaacg ctgtgtggtt acgcttcatc attgttaata cacggtctga accactttga
     7801 atcggtaaat gtaagaaact gaccaattca ggtgtatcac gataaacttc gataatatca
     7861 tcggtaaatt caatcgggtg gctggtggtg taacgtaagc ggtcaatacc gtcaatcgct
     7921 gccactaagc gtaataattc ggcaaaggta caaatgccgc catcaaaagt ttcaccacgg
     7981 taagcattta cgttttgacc gagtaaatta acctcacgca caccttgtgc cgcaagttgg
     8041 gcgatttcaa ataatacgtc atccaccggg cgggaaacct cttcgccacg agtgtaaggc
     8101 acaacgcaga acgaacaata tttattgcag ccttccataa ttgaaacgaa tgctgtaggg
     8161 ccttcggctt ttggctccgg caagcggtca aatttttcaa tttccgggaa agaaatatcg
     8221 acaatcgagc ttttaccgcc acggatttgg ttgatcattt caggcaaacg gtgcaaagtt
     8281 tgtggcccga aaacaatatc tacataaggg gcacgatcac ggatatgctc cccttcttgt
     8341 gaagccacac aaccgccaac accgataatc aggttcggtt tatctttttt ccagtttttc
     8401 caacgcccga gttgcgagaa tactttttct tgtgcttttt cacggataga acaagtgttt
     8461 agcagcaaca cgtctgcatc ttcagggttt tcagtcagtt caaaaccgtg agtagagttt
     8521 aggagatccg ccatttttga tgagtcgtac tcattcattt ggcagcccca agtggtaata
     8581 tgtaatttag ccataacgtt taaaattaat aaataattca ataacttaga aaagaaaagc
     8641 gtaaagcaat ttattatttt tgacttaaaa taaacccttt acgcaaaatt tgtggcgtta
     8701 ttttaccttg ttgcctattt tttatctata aataatcgcc atttatccga ttttacctaa
     8761 aacaaaaaag ccacgatctt ccatcgtggc ttccagctaa gtaggaggta ttatttggtt
     8821 aaggttaaaa ccacgctttc accggcaaca acttggccat ttaatttaga taagttgctg
     8881 atttcatcca tatttgaaat cacgattggt gttaatactg atttggcttt gccttcaagt
     8941 agagctaaat caaatttaat gatcggctcg cccactttta cggtttgacc ttcctgtgca
     9001 aggcgagtaa agccttcgcc ttttaattca acagtatcaa taccgaagtg aacaaataat
     9061 tccacgcctt cttcagattc aatcgaaaaa gcgtggttgg tttcaaaaat tttgccgata
     9121 gtcccattta ctggagcaac gatagtgtct ccactcgggc gaattgccac accgtcacct
     9181 acaatttttt cagagaaaac aacatcaggc acatcttcaa tatttacgat ttcacctgtt
     9241 aatggcgcat agattttgat ttcttgactc tctttttgtt ttgagccgaa taatttatca
     9301 aataaaccca tcttaaaatc tcccttagat attagataca aaattctcgt acattttata
     9361 cgagaatttt gtatttgtat gctaaataat taaaattagt ttaatttctg ataaaactca
     9421 tctactaagc gttcaatatc tgcagcagtt ggctgagcaa gcgcctcatc agctaatgct
     9481 ttagcatcgg caaaattaat tgagcgaatg agttttttaa cgtgtggcac agagataccg
     9541 ctcatactaa attcatctaa tcccataccg agcaacaacg cagttgcacg cacgtcaccg
     9601 gctaattcac cacacatacc tgtccatttg ccttcagcgt gtgaagcatc aataacttgc
     9661 ttgattaagt ttaagactga tgggcttaat gggttatata aatgagcaat aatttcatta
     9721 ccacgatcta ccgcaagtgt atattgggtt aagtcattag taccaatact aaagaagtcg
     9781 gcttcttttg caagatgacg tgcatttacc gctgctgatg gggtttccac cataatacct
     9841 agttttaagt tctcatcaaa agcgtgacct tctgcacgta attgatcttt caattccgca
     9901 acgatggatt ttaataaacg gatttcttcc accgaaataa tcatcgggaa cattaccgct
     9961 aaattaccga acgcagaagc acgtaatacc gcacgtaact gagtatctaa aatttcacgg
    10021 cgggtgaaac cgatacgcac cgcgcgccaa cctaagaatg ggttcatttc tttcggtaaa
    10081 tcaaggtatg gtaactcttt atcacctccg atatccatcg tacgcagaat gaccatttga
    10141 ccattcattg cttcaacaat ctctttgtag gcttgaaatt gctcttcttc tcccggtaat
    10201 tgagtacgat ccataaacaa aaactcagta cggtataaac caaccgcttc accaccgttg
    10261 cgtaatacac cttccacatc acggatagta ccgatattcc ctgccacatc aactttatgg
    10321 ccatctaatg tttgagcagg caactctttt aatttggcta attcttcttt ttccgcattt
    10381 aatttctctt gtaatgcttt gaaattagcg attttttctt ccgatggatt taaatgtact
    10441 tcattgttca ctgcatctaa aatcagaaaa tcaccagttt taactaggga agtaacattg
    10501 ttagtaccaa caatggcagg aagctctagg gaacgagcca taatagaagt gtgagaagta
    10561 cgaccaccga catcggtaat aaagcctaat actttatcta aatttaattg agctgtttcg
    10621 gaaggggtta agtcataagc gactaaaata acttcatctt gaatatcacc aagatctacg
    10681 atattcatat ttaaaatatt gcgtaccaag cggttaccaa tatcacgaat atcacctgca
    10741 cgttctttta aatattcatc gtcaatttcc gcaagcattg aagcctgcat ctcaatcact
    10801 ttacttgctg caacatccgc agtcacaaga ttatcagtaa ggtaactcaa aatttcttct
    10861 tcaagctctt catcttctaa gatcatcaag tggccttcga aaatcgcctc tttttcctca
    10921 cctaatgtac ggcgagcttt ttctttaatc gcggttaatt gagcagctgc tttttcgcgg
    10981 cctgtaaaga attttgcttt ttctgcctca acttcgcttt cagtaatttt gcgagtattt
    11041 agtacaatcg gctcttcttt taaaaccaat gcttttccaa atactgcacc aggggaagct
    11101 gcgatacctg taatcataat tgtgtcctta atttaagatg atgtaaaaat catctggatg
    11161 ttacatagca aaacacttta ttgtagctta ttaagctaca ataaagtgac ataaatctta
    11221 aatccgagaa tagaaaaatt attctaaagt aggaatcaaa tcaactaaga aatcaactgc
    11281 tttttgctca tcttcgccgt ccgcagaaat tgtaattgtt gtgccttgag tcaaacctaa
    11341 tgtttgtaat ttgaataagc tttttgcact tgcactttta ccacctgaag tcacagtgat
    11401 atcagaagca aaacctttag ctgcttttac aaattcagcc gctgggcgag tgtgtaaacc
    11461 atttggagcg gtaataacaa catcttttga gtacataaaa tcttcctctt aaatttgaaa
    11521 gttaaaaaaa tttgttttaa tccatttatt ttgcgcacgc aactttacta caaattcaaa
    11581 ttaaaaacac gctaaaaagc gcaaaaactc gtcgtatcac cctgctaaat agggtgtttt
    11641 ttaagctaac ttaaaaatca tttgtcgaat atcaagattc tagcacacac ttaagcggaa
    11701 atgttacaat aatttcgcta ccacttcttt cacttatatt ctaaatttgg agcaatatgc
    11761 ttagcacatt aaccaaaaca accgcatggt ttatctactt tagtattatt gctctctatg
    11821 tttttagtct atgggcgtta atggactatc accaaaatga taccttattt gttttttccg
    11881 atataacggc cacggtcgct tatagcctac ttcaagccag cctctctgcg gtaatttcac
    11941 tatttttagg cagcctactg gcaagatctt tcttttattt ggactttaaa ggaaaaaatt
    12001 tactctataa aaccatttcg tttatctggg cattaccctc actagtaatt atttttgcgg
    12061 taattggcgt atttggtaac tcaggctggc tagccaaact tttccaacaa ctcggttgga
    12121 attggcaatt taatttatac ggcttgcacg gcatcatttt ggctcactgc ctgtttaaca
    12181 ttccgtttgt gatgaaatat tataccggag ggcttcaatt gactccaagc agtcgctttc
    12241 agcttgcggc tcaattaaac ctaacaggtt ggaattatat ccaaattgtg gaactaccga
    12301 taatcaaaac tttgctgcct tatatgttta tgactgtttt tctggtctgc tttaccagtt
    12361 ttccaatcgt attaatgtta ggcggtagcc caaaatacag cacgcttgaa gttgccattt
    12421 atcaagcggt cacttttgaa tttgattttg caaaagccct catcttaatt agggtacaat
    12481 ttgtagtggg cattggctta caattagtaa tgaattatat ctcgacttct gcactcaaaa
    12541 agctaaaaca gcaacctaac cgagtagaaa tttggaaacc aaagcctacc ggactggcta
    12601 aactcggctt acaagcggtt atttttttgc aaagttttgc aataattcta ccgcttgtca
    12661 gcgtcatttg ggcaggacta agcgtgtcaa aactcagtga gcgtttgctc aaccctactt
    12721 tatggcaagc tactcagtat tctttatact taagtttaat tgcttctata ttcgtaatca
    12781 caatcagcta tttgattgca cttgaagctc gccaattact ctatacaaaa gccaagctca
    12841 gatatagcat tctcaccgca atcaccctat tccccttact tctgccgatc tttttattat
    12901 cggttggttt gtttatttta ttaatgaata gtgaactctc aagcgaggca ttactgctac
    12961 tggtcgggat atgcaacggc ttaacattac tgccatttat ttacccaatg cttttctccg
    13021 caatgtggaa tagcctcacc tcgcacgaca aattagcaca aggacttggg ctaaaggggc
    13081 ttcgccgttg gtggattgtg gaaaaaaatc aactaattcg accgcttgtt accgtatttg
    13141 cgttagcaat gtcatcgagc ttaggcagtt tcaccgtcat tgcttttttc ggcaacgctg
    13201 atttcagctc actcccttat ttactctacc aacaacttgg tagctataga accgaagatg
    13261 ctgccgttac cgcactcatt ctgatgttat ttgcgttact accattctta ttcagcaagg
    13321 ataaactatg atcgaactgg atctccattt cacttacgaa caaatgaata tgcactttca
    13381 gctttctgtc aaaaaaggcg aacgtattgc agtggttggc gaaagtggcg caggcaaaag
    13441 cacattactt aatctaattg ccggttttga aaaggcagaa agtcagcaaa gccagctgtg
    13501 gctaaatcag aaaaatcatc ttgataccga agttgcacaa cgtcctgttt caatgctatt
    13561 ccaagataac aatattttcc cgcacttaac ggtagaacaa aacatcggct tagctttagt
    13621 accaagtttg tcgctcaatc cgaaacaaaa agcacaggtt aaacaaattg ccacacaaat
    13681 ggggattgaa accttccttc agcgtcaagc cgatcagcta tcgggcggac aaaaacagcg
    13741 agtggcatta gctcgtactt tgttgcggga taagccgatt ttattattgg acgaaccctt
    13801 ctccgccctc gacccgcaac gccgagccga attacagcaa atcgtgtttg acatttgcca
    13861 aagcagaaat ctaaccctgt taatggtaac tcaccaacta tccgaaagcc tatttctatt
    13921 taatcgggtg ataacggtag cagatgggga gattgtttct gatctctccc ctctttagca
    13981 aagcagatct gctaggttct aaatgtaggg acgccacgct ggcgtacgca acgatgtcaa
    14041 ttccaacaat ctaacggtga atgatgttcc gcacggatac tagcgttatt catttttttc
    14101 agaactcgcc ccctacttta gcaaagaggg gaactgtaac taatattacc tccctattcc
    14161 gctattctct tccccaaaat gtgatctact tcacaaattt ggaaatattt taaatcttct
    14221 cttttttagc taaaccgatt tagttaaaat gcaaccgatt attttttatg aaaggtaaat
    14281 tcaaatgaac tatccaaaaa ttgcagaaca aacgattgca aaattaggtg gcaaagaaaa
    14341 cattaccaca cttgcccact gtgcaacccg tttacgctta accattgctg atgaatccaa
    14401 aattgataaa gaggcgattg aaaatattga aggcgtgaaa ggtcaattct ctactcccgg
    14461 tcaataccaa attatttttg gatcaggcac ggtaaatgaa gtgtatgccc aaatgcaaaa
    14521 acaaatgggt atggcagata tgtccacctc tgaagtagcg gctgccggtg cagcaaatca
    14581 gccattacta caacgtttgg taaaaggctt agccgatatt ttcgtgccga tcattccggc
    14641 tattgtggct ggcggtttgt taatgggtat tcactcaatg ctaacggcag tcggtttctt
    14701 ctgggaaggt gaatctgttg ttagccgtta tccgaatatt tccgatttag ttgatttcat
    14761 caacactatt gctaacgcac catttgtatt cttacctgta ttactcggtt tctcagctac
    14821 acgcaaattt ggcggtaatc cattcttagg tgcggcactt ggtatgttat tggttcaccc
    14881 ggcacttgca gacggttgga actatgccaa aaccttaatg gaaggtaaca tcaaatactg
    14941 gaatattttc ggcttagaaa ttgaaaaagt gggttatcaa ggcacggtta ttccaactat
    15001 cgtttcggct tgggtgttag caacccttga gaaaactttc cgtaaatttg tgccaagcta
    15061 tttagataac ttggtgacgc caatgatgtc attatttatc gccggtattt tagctttcac
    15121 tgtaatcgga ccattgggtc gtgaagcagg ttcagcgatt tcagcaggct taacgtggtt
    15181 atatgacagc ttaggcttta tcggtggagc aattttcggt acattctacg ctccaatcgt
    15241 gattaccggt atgcaccaaa cctttattgc ggttgaaacc caattattag ctgaagtagc
    15301 acaaaccggc ggtacattta tcttcccgat tgcagcaatg tctaacattg cacaaggtgc
    15361 agcctgttta ggtgtagctt ttgcaatgaa agatgccaaa gtgcgtggtt tagccgtgcc
    15421 gtcaggtatt tcagccctgt taggtatcaa ccggcaatgt tcggagtaaa tttacgctac
    15481 cgccaagcct ttattgcggc gatgatcggt tcaggtttag ccagtgcatt tatcgctttc
    15541 tttaatgtga aagcaattgc cttaggtgca gccggtttct taggtattcc gtcgattaaa
    15601 cctgacagcc ttgcaatgta ttctgtcggt atggtaattt catttgtagt tgcatttacc
    15661 atttcagcca tttgggtgaa aaaagcacaa gcgaaataat caaaaataga tcacaaaaaa
    15721 gggattaagt gttagcttaa tcccttttgt ttttaaacgc cttagcttaa caatgagttt
    15781 cgcaagcggt catttttcgc taaaaatttg caaattagct gttgtgcata attcgagaaa
    15841 tacgcccttc ggcaatctct tcatcacgaa ttgtcagcac ttcacagccg tctttggtaa
    15901 ctacaatttg atgctcatat tgtgcggagt ggctgcggtc tttggttttc actgtccaac
    15961 cgtcacccat taagcggact tcttttttgc cggcattcac cattggctca atcgtaaaca
    16021 ccattccttc ttttaacact acaccgccat catcggtagc ataatgcact acttgtgggt
    16081 cgcaatggaa ttcagtacca ataccgtgtc cgcaatattc tcgtactacc gaaaagcctt
    16141 ctttttccac atatttttga atcgctgtac caatttcttt taaacgcacc cccgctttca
    16201 cggtgcgaat cccgacataa agggactcta gcgtaaccgc cattaagcgt ttatcccgca
    16261 cacttggctc accgactaca tacatcatcg agttatcgcc atagtagcca tctttgataa
    16321 ccgtcacatc gatgtttaaa atatcgcctt ttttcagctt tttgtcatca cttggaatac
    16381 cgtgacaaac gacctcattt aacgaaatgc acaccgattt tgggaagccg tgatagccca
    16441 aactggctga agttgcccca tttgccaaaa tgtattcgtg acaaatttta tctaattcac
    16501 cagttgttac gccctctttc acataagggg cgatcattac taaaacatct gaagccagtt
    16561 tacaggctac tcgtagtttt tgaatttctt cttcgtttct taatggaata ttattcatta
    16621 cattgctctc tgctatatct gccataaaaa gtaaggggat tatacctttt ttcagcacaa
    16681 tccccaaacc tcagtttaga tttctacttt aaatcaagtt tcttcataat gaaatttaat
    16741 atcacaccat aaagcggtag aaatagcagt aggctcacaa agagtttaaa cgcataatcg
    16801 accgaaccaa tagtaaacca atgctctgcc atatattcat cgctactttt ataaaaggcg
    16861 atagcaaaga atacaaaagt atcgattaaa gtaccaaaaa tggttgaaca ggttggagca
    16921 agccaccaac gcttaccttg tcttaaacta ttaaatacta gaacgtctaa taattgccca
    16981 actaaataag ctgcaaagct agccagtgca atacggaaaa caaaaaagtt gaactcagtt
    17041 aatgcactaa agccttgaaa ttgggtttca aagaataaaa cggaaatcac atagcttatg
    17101 acaagtgccg gaaacatcac aacaaaaata atttttctag caagatctgc accaaataca
    17161 cgcacggtta aatcggtcgc taagaaaata aatgggaatg ttaacgttcc ccaagttgtc
    17221 ggaaagctaa attctgttag tggtacttga acttcaaagc taatttgcac taaatagttg
    17281 ctaattgcga tgataaaaat atggaaaagt gaaagtaaaa ttagagaacg acgtttgttc
    17341 tcatccgaaa aataagtagt aaagttcata ttgtatcctt tttcgttaaa tgaaaacttg
    17401 aggtaaggga actcaagggg gcattattct atacattttc ccataaaaat caagctcata
    17461 ttatttcaaa aaatgaaaca attaatctat taaattaggg tttatctatt tttgacataa
    17521 agtaaaatgg cacaacttaa cacttacata aggaatagat aatgttacaa ctgagaaaag
    17581 ccaatgaacg aggcgatggt tcttttagct ggcttaaaag ctaccacact ttttcctttg
    17641 ccaattatta cgacctaaaa catatccatt tttcacattt acgggtaatt aacgaagatt
    17701 tcattgcacc taatcacggt tttggcaagc acccgcacga taatatggaa attttaactt
    17761 atatgttaaa aggacgtgtg gctcacgaag acagtatggg taataaaacc gaagtaaaag
    17821 ctggcgaatt tcaaattatg tctgccggaa ccggggtttt ccattcagaa attaatccag
    17881 atccgaatga aaccttacac ctttaccaga tttggattat cccaaaccaa aaagatgtga
    17941 cccctcgcta caaccaagat agatttgccc cgaaagaggg agcaaccttg attctttcgc
    18001 caaacgcaga agaaggttca tttaaaattt accaagatat gaagctgtgg cgttatcaat
    18061 acccaacagc caaatcagag caactggcat tagagccaaa ccgcagttac tggctacaag
    18121 tggtgaaagg gaatctaaac atcaatggcg ttgaactttc caccagcgat gccttaggca
    18181 ttcgtcaaga gaaagcactt gaaataaaca ctcaagatga cgtggaattt ttactgtttg
    18241 atctagtgta aatacaagag acaagcggtt aaatttgtaa aaaatattac taattcagct
    18301 gcttgctctc attaaacatt atcgcaccaa aattctcact cctagcgttt cattttgttg
    18361 aagtaaggta tttatccgtg ctgtttccac acataccata ttttgatacg ccgtttctgt
    18421 catcgcacta acggtattat gccaaggatt ccaaagtacg gtttcggtag cattaatatg
    18481 ctcaacttta atggtgcgct gatttgcaaa atcttgaata aaattcaccg cttgtgccgg
    18541 ataaatacaa tccacatttt cacttatctt gcgaggtgat ggcacttcaa tttcttgatt
    18601 tgtcaattta tcaaaacatt gtgttgctaa gccttgtact tccgttttcg taatatcacc
    18661 gacctgaaaa taagtatgca atgcagcctg tgccggctcc ggtgctaagt gagtaaaata
    18721 caactcacag acttcgccta gtatcatctc gagttttgcc tgattttcta accccaaaac
    18781 aacacgaatc ccatctgctg aggaagtatg ttccagcaaa ttccacgctt gaatacgggc
    18841 tgtgccgtga gcaggctttt tgtctgtacc aaaccaaggg tggcaaatcg gaatgccgcc
    18901 acgaatcgcc acacctgttt ggaaaggttc aatttcactt aaccataata catcttgctg
    18961 agtattttta ggttgccaac tcagtaactg cgcaccttgc aatgcaattt tagccttcac
    19021 tttggagtga ttaatctcta aaacatccaa ttcattatat tttgctaatg ctatttcagg
    19081 gctgatcatt ttgattcgta gattattcat cgtattgctc ctcatcttga aagataaaat
    19141 gtgaagtgga tcacagaatt aaagcttaaa ccgctacctc tttaaaggta atcgttataa
    19201 taagggcatt ctaacatagc gaaggagaaa aaaatgaaaa cactcggcga atttattatt
    19261 gaaaaacaag cagaataccc aggggcaaaa ggggaattaa gtgggatttt atcttctatt
    19321 cgtttagccg ctaaaattat tcatcgagaa atcaaccgtg ccggtttaag tcaagatatt
    19381 ttaggtactg caggttctga aaatgtacag ggtgaagccc aaatgaaact tgatgtattc
    19441 gccaacgaaa cgatgaaaaa agcgttactt gcacgtgaag atgttgccgg ctttgcatcg
    19501 gaagaagatg ataattttgt tgcctttgat aatgagcgtg ggagaaatgc aaaatatatt
    19561 ttaatgaccg atccgcttga tggctcctcc aatattgaag tgaatgtttc tgtgggaaca
    19621 atcttctcta tttaccgccg tgtctcccct atcggcaccc cagtaacaca agaagatttc
    19681 ttgcaggaag ggcgtaaaca agttgccgcc ggttatgtta cttatggttc ttcaaccatg
    19741 ctggtttaca ccacaggcaa tggcgtgaac ggttttacct atgatccgtc attaggctta
    19801 tttattcttt cacaccctga tctaaaaacc cctaccgaag gaaaatatta ctccatcaat
    19861 gaagggcagt atgttacttt cccacaaggt gtgaaacgct ttattaagta ttgccaagaa
    19921 accgatgaag ccatcaaacg tccatactct tcacgctata tcggctcgct ggtgtccgat
    19981 ttccaccgca atttattaaa aggcggtatt tatatttacc caacctccac cgtatatcca
    20041 aaagggaaat tgcgtttact ctatgaatgc aacccgattg cgtttcttgc cgaacaagcc
    20101 ggaggaatgg cgacagatgg tattaatccg attttagata ttaagccaac agagctacac
    20161 cagcgtgtac cgttctttgt cggttcaacc tcaatggtca aacaagcggg cgaatttatg
    20221 caagaatttg caaaataatg ctcctgctta tcactcaata aaactttcaa caaagcagct
    20281 gcgtattccg tagctgcttt tgcctatctt aagattgtaa atttgtataa aaaacaggta
    20341 agacctcttg ccaaataaat aattatctat agaatacccc acctttttac tccattaata
    20401 ttagcaattt attaacattt tatgataaat aaaaactttc cactttctta ccttgccagc
    20461 tttgtggcat ttgctgttag tttatctgca acagcctctg ttttcagaga tgatatcgat
    20521 cttcaatact accgtgattt tgcggagaat aagggtaaat tcaccgtagg tgcaagcaat
    20581 attgaaatta aagataaaag tggcaataca ttagatgcac tcttcccaaa tgtaccgatg
    20641 ccagatttta gtgctgcaaa ccgtaactta gggatagcaa ctctagttgc accgcaatat
    20701 ttagtcagcg ttgcccataa tacacaatac aatactgtgg aatttggcgc cccaggcact
    20761 aatgccgatg cacaccacta cacttacaaa gtcgttgatc gtaacgacta tggaattgtt
    20821 gaaggtgggc aacatcaaga ttatcaagtg ccacgtctca ataaattagt cactgaggtc
    20881 gcacctgcaa cagtgactga tctgggtaat aatgcttcag cctaccaaga tagtagtcgt
    20941 tttactcact ttgctcgctt aggctctggt cgtcaaatag tcaaaaatac cgagcataaa
    21001 gacaaccaaa tttcaacatc ttaccaatat ttaaccgggg gagttcattt accaatcgcg
    21061 gtacataatt ccgattactg gctagatttt agaggaaatg cattaaacaa tagcccatat
    21121 ggagcattaa ctgcatttgg tactcgtggt gatagcggtt caggtgtgta tggctacgat
    21181 cagaaaacga aacgctggct actactggct acttacacct ttggtacgcc tgcaaacaac
    21241 tactacaacc gtgccggtat tatccgtcag gattatcacg ataaacaatt tgccgaagat
    21301 attgccggca cattaaccaa tgcgcaacaa aatgccgttt ttgaatggtc tgcacagggt
    21361 aaagacagta gcattggtaa taaagggaaa aacttaacgg tttcgcttgc tgatcgttca
    21421 attaaagaca atagcgcttc tccattaggc tatggtggca cacctcagct acaacttccg
    21481 caagataaca ccggaaaaac cctaaacatt gaaggacaag atagcactat cgtattaaaa
    21541 accgatattg accaaggtgc tggcgcatta aatttcaatg ctaatgcaac agttcgccct
    21601 gaaaatgatc aaagctgggt aggagctgga attgtagtgg caaaaggtaa acaagttaat
    21661 tggcaagtca aaaacccaca aggtgaccgt ttatctaaat tgggcgaagg gacactacat
    21721 attaacggta aaggcgaaaa ccttggtgac attagtgttg gacaaggcac tgtcattttg
    21781 acccaacaag cgggtgaaaa tggcaaaaaa tctgcattta accaagttgg tattgtgagc
    21841 ggccgttcaa ccgttgtatt aaacagtgct gatcaagtcg atccaaataa gatttacttt
    21901 ggcttccgtg gtggtcgttt agatctcaac ggcaacaaca ttgcgtttaa tcgtattcaa
    21961 aatagtgatg atggtgcgcg tattgttaat aatcacctac aaaaagctgc aacactcacc
    22021 attaatggcc cgaagccccc tgaagcaacg gacttaaaat ggggaacgtg gaaagaaaac
    22081 agtgctgata tttatgaata tatcaaccca catgctaata accgtaccga ttactttacg
    22141 ctcaaaggca atccaaacca atatatgcca acgaatggcg caagtaatgc ccattggacc
    22201 ttcctgtctt ctaacaagga tgcagcagta aaacaagtac tcgcgcaaaa aggtctggag
    22261 catcgctaca atagctttaa tggttttatt ggcgaaacag ataacaccca gcataacggt
    22321 cgtttgaatg tggtgtatga tccaaaagcc tctacaccac cggcaactgc ctctgaagta
    22381 acttggggta aggggctagt agctggtgca gatatttatc tgttcaccaa tccgaaaacg
    22441 aaaattcggg aatactttgc attaaaaggt gatccaaaac aacctgttcc tcgcggtggc
    22501 ttaagcagcg agcattggga atttttagct gcagatcgca atcaagcgat caacaaagta
    22561 ttagcccgta aaaacgcacc aatagagcaa gaaaagctca atagtattta taccttctct
    22621 ggtggtttga acttaaacgg cgatctaact gtgaaaggag gtaaagtgct actttccggc
    22681 agaccgaccc ctcacgctta tgatgcgatc aataagcaag atgtagttta tgccgatgat
    22741 tggcaaaatc gtcaatttaa ggctgataat atgcagctaa accaatacgc tcagctttat
    22801 gttggtcgta atgtctcaaa cctgcaagca aacttgtcag cgaatgacca tgctcagcta
    22861 catttaggct ttatcaacga gcaaactcca agttgctact attctgagta cactggtaaa
    22921 acaagctgtg atacacaagc ggtcgtttct aatgaaattt ttgcaacact gcccacaaca
    22981 caaattaatg gtgatgtcag cttggcagaa cagagccaat tacacatcgg caaagcgaac
    23041 ctgattggca ccattcaagc agcagcaaca acgagcattc gtttagctaa ccaagcaagc
    23101 tggacaaaca ctggggatag ccgtactggc aatttagtgg cagaaaacgg ttcaaccatc
    23161 aacctgaatg aaaaatttgt aacaggcgag ataccgactc gttttaatac gttgattatt
    23221 gacggtaatt tccaaggtaa tgccaaaatc aactacttaa ccgatattgc tgctggcaaa
    23281 ggcgatcacc tacaagtaaa tggtctcgcc gaggggactt tcactcttgc attacgcaac
    23341 agtggcaaag aagctgaagt ggttagcccg ttaagtttat taaccttaac ccatcaggct
    23401 caggctgata aagctaaagt ttcactggaa aacggttatg ttgatttagg tgcatatcgt
    23461 tatgtgctgg caaatcgaag caacggctat cgcttataca atccgttaaa agatgctaaa
    23521 gatcggaatc agtcgattgc aacggcgaaa gcggaattag accatgctat tgcagaagcg
    23581 gataaacaaa aacaggaaat cagccgtttg aacgcagaag ccgagaaaga gcgccaagca
    23641 gagaaaaatg ctaaacaagc cgctgcaaac gcacaatctc agcttagcca agcaaatagc
    23701 gaattaaccc gtttgcaaca atatgctgat tactaccgcc gttactatcc aacgtattac
    23761 cgccaaatcc aagggcaaat tacggtagca aaacagaaag tcacacaagc gagtgctgca
    23821 ctcactacag cagaaaacaa tgctaaagca tccgctgcgc aaattgctaa cgtagaacaa
    23881 gcggttgaaa ctgcacaaaa tattgcaaaa caagtagaag caaaattagc taccttacgt
    23941 ataactgctg gaaatagtga agcaatttta aacgctgagg cactaaaact ttgccaagag
    24001 aatggtgcaa attgtgagca tctacaagca cacgcagaca ctgacagtag taatattcaa
    24061 caaagcgact gggtaagcca atatgccaat accgctcttt ccgagctatc ggcacaggca
    24121 aattctgcgt tgcaaatcgg gcaagactta gatcgccaac tgttcgcaaa acacgataaa
    24181 ttccacgttt ggagcagtat cgaacatcaa aaaaccgaac accaatcgga tttatatcgc
    24241 ccgtatgagc aacaaaccaa cctcactcag ttaggcatag aattaccgct tgcaaacggt
    24301 atcaatgccg gtgtgatgct atcccgcaac cacgctaatg cggaatttga tgagggagtg
    24361 aacggtaaat ccaacttact gatggcaagc ctatatggta aatggcattc ggaaaacggc
    24421 actttcgtca gcctagacgg tagctacggt aaagcgaaaa accgtatcga cctgttcggg
    24481 gagaaccgct ttaaccgtca tattatggcg attggggcaa atcttggaca taacttcgac
    24541 ttagccggcg tacaagttca gcctgctgtt ggtacacgtt actatcgctt cagcgcacaa
    24601 aattacaaac tgggtgaagt agaagtccgc agcccgaaag cacactttat ggcctaccaa
    24661 gcaggtgtga aagtcagcaa ggcctttgac ctctccagct ggaaagttga gcctagcctt
    24721 gccgcacatt atgtcgatgc cagcagcaaa cgcttgagtg tggcagtcaa tgacaatacc
    24781 tttagccaac gtttcggacg ttatctgaaa actgaagtgg gcgtgggtgt tacaaaaggt
    24841 caatggcaat taagctctca ccttggtcta ttgaaaggta acgaaatcgg caaacaacat
    24901 tttgccggct tcaaactggg atatagctgg taattgctat tagcccctaa actaagcaat
    24961 cgcttacttt aggggctttt tagtcaatta attagccggc tctttgcttg cctctagcca
    25021 ctgccgaata aaatcagcaa gctgttcaac atcattaata tccaaccgat tttcccgctc
    25081 aagcggataa tcggttgcgg tcgcgattgt ccactggtct aacgccggca acggtttttc
    25141 aatttcttta cggtgtagtt gaattttcgg cattgtttcg tgcttaaagc cttccactaa
    25201 aactaaatct accgtctctg ggtcaaattt tgcaattaat tgtgaaaatt cgaccgcttg
    25261 tttagtttct gctgcctgct tagtttccgt cattaatgcc caacgttcat cacacacgac
    25321 catagtcggg tttgctcctg cttctcgcaa gcgatagctg tcttttcctt ttttatcaat
    25381 atcaacattg tggtgggaat gcttaatcaa gccaacccga ataccgcaag cggtcaattt
    25441 tgggattaat ttttccaata aggtggtttt gcctgtgccg ctatagccag taatcgccag
    25501 ggttttgatg ggatatggat tgaaagccgt taaatcttct aaacaattaa aattgcgaaa
    25561 tgcggcttta tatgccgaga aatccactgc cacacttttt tgggtttgaa aaaaccacaa
    25621 taatcggcgt tcgccggagg ctaaatacgc tcttaaattt tccgccaccg aacgatgcac
    25681 caaggcaacc gttgggtgtg ggcggtcgcc atcgtgagca taggcaattt gagcctgatt
    25741 aatgcgtaat gccgtgtgta acttgtgcaa tagttgggtt ggaataaacg gcgtgtcaca
    25801 aggcacaaac agtaaatagt cgctctcaat ctgctcgaac ccggaaagca ttccacttag
    25861 tgggccttga aaatcgggca agctgtcttg gtaataaggt aattcagggt aaagttgttg
    25921 atattgttcc accgaacgat tgatattgag ccaaatatcc tcgatttgtg gcgaaagtcg
    25981 ggctaacagg tgcgaaatca acggtttttg atctaacagt tgcaaccctt tttccgctcc
    26041 attcattcgc cgagccaaac ctccggctaa aattaccgct gaaattttct cttccatttc
    26101 cctctccaaa cctgaatttt tttgccaaat gagggctaaa agtatattat tccgcttact
    26161 attcaacaag ttttttaaga ggcatactat gaaatgcaaa cgtttagaag agctattgga
    26221 actgcttggc gaacattggc ggaaaaaccc cgatcttcac ttaattgata ttctgcaaca
    26281 gctttcggtg gaagtaagcg agccggataa ttttcatgcc ctgcgtgacg aggttttgat
    26341 ttatcagctc aaaatgcgaa acgccgataa atttgagccg attccgggca ttaaaaaaga
    26401 ttatgaagat gattttaaaa ccgcattgct ccgagctcgt ggaattttaa acgattaagt
    26461 ttgtcttatt tctcacttat catatttttg aaggaatcat tatgaaaaaa tttgcattaa
    26521 aaaccgtact ggttgctctt tctgcccttt ttgcatttaa cgcaaccgca gttgcagcag
    26581 atccaactgc cggcaaagag tatattgagg tgcgtaaagc cccatcggct caaaaagaag
    26641 tggtggaatt tttctcattc tactgccccc actgttacga ttttgagcta aattacaaaa
    26701 ttccaagcca aatcaaagcg aaattgcctg cagacagcaa attagtgcaa tatcacgtca
    26761 atttcttagg tcgccaatct gaaaacttaa cccgtgcttg ggcattggca atggcattag
    26821 gagcggaaga taaagtaaaa accgccttgt ttgaagccgc tcaaaaagat gcgtttaaat
    26881 caatggacga tattcgtgcg gtcttcatcg ctaacagcat tagtgcagaa cagtttgaca
    26941 gcggtatcaa cagctttgca gtaaacggtt tagtcaataa acaagttcaa ttagctgaag
    27001 acttccaaat tcgtggtgta ccggcgtttt tcgtgaatgg tcaataccag cttaatctag
    27061 aaggattttc cgattcaagc tcgaccaatg attttatcca acgctatatt gacgctgtgg
    27121 tcttcttgag taagaaataa tcgtccccat attaaggaga tggttttaca agtgctgaat
    27181 agtaaagcta ttcagccttt tttatatgca aactcggaga acaaaatgac aaacttatcc
    27241 attctcaacc ttgtgccgat tcgtgaaggg caaactgcca aacaagcggt cgattctatg
    27301 gtgaaattag caaaacacgc agaaaatatc ggcattgaac gctattggat tgccgaacac
    27361 cataatatga aaaatcttgc cagttccgcc acgcaattat tgattcaaca cgccctctcc
    27421 cacaccgaaa aattaagagt cggctccggt ggcgtgatgt tacccaacca cagcccttat
    27481 attgtggcag aacaatacgg cacgcttgaa acactctacc caaaccgagt agaactgggg
    27541 cttgggcgag cccccggcac cgatatgcga accgcctctg ccttacgccc agagtttgcc
    27601 tttccccgat gaaatcagcg aattaaaagg ctactttgac ggtacaaacc ctgtttccgc
    27661 ctatccggca caagggctaa atgtgccgtt ttatattcta ggctcaagcc ctgaaagtgc
    27721 ctatttagcg gctgaacgag gcttgcctta cgccttcgct tcacactttg cccctcgctt
    27781 tatggacgat gcaatccgca tttaccgctc acattttaag ccctctgatg tgttagataa
    27841 accttatgtg attttaggtg tgaatgccat tgtggcagaa accgatgaag aagcaacaca
    27901 actggcgacc acccaaaccc agtttttctt aaatgtagtg accaatgccc aacaaaatct
    27961 gcaaccaccg ctcgaatctg acgacaaagt gtgggaaaat tttgtgaaag cagaaattgt
    28021 gccgcatttc gggccggttg cctttgagca aaatgctcta taccgacaag agaaaaccgt
    28081 tgtccgccaa atgaccgaat gctccttaat tggtagcccg caaagcgttg aacaacaact
    28141 taaagcccta aaagcacgag tggaaattga tgaaattatg gcggtaaact acattttcga
    28201 tgagcaaaaa cagcatcaat cctacacttg gttaaaacaa attacggata aggtgtaata
    28261 tgagcaaaac gatgcaacaa aaagcggctg aggcacataa tatgtgccgg ctaaaaggcg
    28321 attctatgtt ggataatgca gaccgccaaa ttgcctttga agcggtgtat gagagcgaag
    28381 aactggctca acaagcggtc gaatttttcg cacaaaaagc aaaatcggtc gaaaccgaac
    28441 cttgtgaaat tcaaagcgaa attacacaaa ttgcagaagg gtttctgatg aaaatgtgga
    28501 tcactttcag ctgccaagca gaagtgattc tgttccaaat ggcagtaagg tagatataaa
    28561 caagcggtta tttttgatga aatttttgca aaaaatcttc agaaaataac cgcttgtatc
    28621 attatgcctg tttaaccgca gccgattcac tacttggttt acgccgttta ccgatatttt
    28681 tggtatcttt gtggcggact tttacttttg gttttgccgc ttcttttttc gcctcttttt
    28741 tcttcttaat gcgtgctttt tctttcttgc tggtcacatt tagctcgcca tctttcgggg
    28801 ctttggtgcg aggctctaag ccctcgataa tgcgaggttt tagtacttct tcggtgtaac
    28861 gtttaatttt gccgagtaat ttataatcgt gagcttccac caatgaaatt gccgaacctt
    28921 ttttgccagc acgagcggta cgcccaatac ggtgcaagta agtatccgca ctataaggta
    28981 aatcgtagtt cagcacgaaa tcaatatcgt ctatatcaat gcctcgagcc gccacatcgg
    29041 ttgccactaa cacattcact acgccttctt ttaaacggtt aatagcttgg ttacgttggg
    29101 tctgagccat ttcaccttcg agataagtag aacgaattcc ccgcttacgc aaggtttcgc
    29161 tgagttctcg cacatcttct cggcggcgga caaacacaat cgctctttcc accgaaaatt
    29221 cgctgataat acgagctaat aatttggttt tatgctctac attatcggcg tggtaatacc
    29281 attgctgaat ttttttacgc tcacggcgac ttggttcggc atcgacttta atcggatcat
    29341 ttaataatcg ctcggcaaaa tcctctaata actcgccctc gagggtcgct gaaaacaacc
    29401 aagtatgctt acgccaacgg gtttctgccg caattttctc cgcatcttgc ccgaagccca
    29461 tttgcaacat tctatctgct tcatcaaaaa tcagcacttc caccgctcgg cagtcaaaat
    29521 tttcctcttt aatgtattgc attaaacgac ccggggtagc aaccacaata tcttgatttt
    29581 tattaaagac ttcaccgtga ttttgatacg ccaccccacc ggtaatagtc gcaatgctca
    29641 actgagtaaa tttagccagt gcttgggctt gctccgccac ctgcattgcc agctctcggg
    29701 ttggggtaag aattaaaatg cgaggagccc ctggtttgcg gcgaggataa tctaataaat
    29761 gctgaattgc cggcagtaaa aaagcggcgg ttttgccggt accggtaggg gctgaaccga
    29821 gcagatcacg tccttctaac gcagccggaa tcgtggcttg ttgaatagcg gttgggcggt
    29881 tatagccttt gtgtgctaag gctttaagta attgagggct taaatcaagc tcctcaaacg
    29941 tcattaattg ggtcatctct gtttctctac ttgtaaattt gccccagtat atcacattaa
    30001 ctgcttgccg tgatggtgaa gaaatttatc ctaaaaggtt gaaaaaattg ttacacttac
    30061 acagagccgt ttaacggaca attcgctcac ttttacttta tagaaggaac attatatgtt
    30121 tgttgaaatt tatggacgtt tatcttgccc atactgcgtg cgtgcaaaaa cattagccga
    30181 gaaaatgaaa caagaattaa gcgattttga ttttaaattt attgatatga tcgctgaaaa
    30241 tatttcaaaa caagacctag agcctcgtgt tggcaagccg gttgcaactg taccacaaat
    30301 ttttgtggat aataaccacg ttggcggttg caccgatttc caagcctttg tggaagagaa
    30361 atttgggatt accgtatagc caaaagcccg ctaacaagcg ggctttttta tacaaaaatt
    30421 tgcaaattat tgttcttctt tcggcagacg tttaatcgca aaaagtaaac cgccccagat
    30481 aatcgtcatt gccaccacca tggtaataat tgctgctgaa ctcattactc atctcctttt
    30541 ggttcatttt tccacgccat ttttgatagc aaataagcca ccaccactaa gccgattgcc
    30601 attccccagc cgaaggtgtt tacaaaccag cttgggtagc cttcataacc ttcacgatac
    30661 actttggcga tttcgctgaa taacataaat gccagtacgc cggaggtaat aacgatacat
    30721 aaacgccaga taaagccgac tttgaatgaa gaaacttcgt tcaggtgttt ttcaagggtg
    30781 cttaatttct cgctggtgac aatcgcaatc aaggaaacaa acgccaccgc aacaataccg
    30841 aattggttta cgaatttatc catcacatca agcattggta aaccggtggt tgtaccgaat
    30901 agcaagacag ataccaccat catcggaatg ccgactagta gcgtggcttt cacacgacct
    30961 gaattgagtt tgtcttgcac cgctgaaata atcacttcaa ttaccgagat aaatgaggtt
    31021 aatgccgcaa aggtgagtga accaaagaac agcacgccaa ctaatgcccc cattggtgct
    31081 tggttaataa tggttgggaa agcgaagaat gccaagccga taccgccttt tgctacctcg
    31141 ttcacttctt gccctgcagc cgttgccata aagccgagtg ccgcaaatac gccaataccc
    31201 gctaataatt caaaaccact gttcgcaaaa cccaccacta agcctgaacc ggttaaatcg
    31261 gtttcttttt tcaggtatga agcataggta atcataatcc cgaagcagat agagagtgag
    31321 aagaagattt gaccgtaagc ggcaatccac acacttggat cagccaattt ttcccagttt
    31381 ggggtaaata acgcatctaa gcctttcgca gcacccggta gggttaatga atagccgacc
    31441 aacactaaga acatcacgat caactgcggc attagcaggt ttgaggcagc agaaatccct
    31501 tttttgatcc ccatagccaa cacagcaata gcaatcagcc ataccgcaat taacggccct
    31561 gtaaccatac cgacaaattc aaagctcacg ccttgagtaa tatcgcccat ttttaagaat
    31621 tcaccgataa agaaatccac cggtttatcg ccccacgctc cgctgaatga gaagtaggtg
    31681 taacttgctg cccagccgag taccaccgca taataaatcc cgatgatcac gttgatcagc
    31741 acttgccacc aaccgaaggt ttcaaatttc gagctgaaac ggcggaaagc gagcggtgca
    31801 ccaccacgat atttatgccc gatagaataa tctaagaaca gcagcggtaa acctgcggtc
    31861 aaaagggcaa tgatatacgg cagaataaaa gccccaccgc cgttttcata agtagtataa
    31921 gggaaacgcc agatgttccc taagccgaca gcggaaccaa tagccgccat aataaatgca
    31981 ccacgtccgg agaacgtttc tctttgcggt gcgtttgatg attgattaga cacggtaaat
    32041 acctcaaact gataaagttt tatataaaaa agaattgagt taaatgtaaa ttaatcacac
    32101 tttaagtgcg gaatatgccc aatttcaggt tgaatgtaaa gtagaaaaat atagaaaaac
    32161 agataaaaaa cgctcaattt tcaatataaa actccaaata aaaacaaaaa ttcaaacaaa
    32221 aaaactaaaa acaagacatc ttcagttaaa tataaataaa aacctaaaag ctaccagcat
    32281 aattat
//
LOCUS       DANLXQ010000009        31230 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.009, whole genome shotgun sequence.
ACCESSION   DANLXQ010000009 DANLXQ010000000
VERSION     DANLXQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 31230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31230
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.009"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            240..968
                     /gene="ttrB"
                     /locus_tag="PXD94_000333"
     CDS             240..968
                     /gene="ttrB"
                     /locus_tag="PXD94_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL6285236.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVMVINNEQCIGCGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
     gene            970..2001
                     /gene="nrfD"
                     /locus_tag="PXD94_000334"
     CDS             970..2001
                     /gene="nrfD"
                     /locus_tag="PXD94_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL6285237.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWRSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            1991..5086
                     /gene="ttrA"
                     /locus_tag="PXD94_000335"
     CDS             1991..5086
                     /gene="ttrA"
                     /locus_tag="PXD94_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL6285238.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIINGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGAAYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALTDYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            5227..6072
                     /locus_tag="PXD94_000336"
     CDS             5227..6072
                     /locus_tag="PXD94_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6285239.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(6185..7582)
                     /locus_tag="PXD94_000337"
     CDS             complement(6185..7582)
                     /locus_tag="PXD94_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL6285240.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(7672..8295)
                     /gene="gmk"
                     /locus_tag="PXD94_000338"
     CDS             complement(7672..8295)
                     /gene="gmk"
                     /locus_tag="PXD94_000338"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL6285241.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            complement(8408..8881)
                     /gene="gpt"
                     /locus_tag="PXD94_000339"
     CDS             complement(8408..8881)
                     /gene="gpt"
                     /locus_tag="PXD94_000339"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL6285242.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            complement(9027..10436)
                     /locus_tag="PXD94_000340"
     CDS             complement(9027..10436)
                     /locus_tag="PXD94_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6285243.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            complement(10632..11762)
                     /locus_tag="PXD94_000341"
     CDS             complement(10632..11762)
                     /locus_tag="PXD94_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL6285244.1"
                     /translation="MSIKKVTLASLILLGLTACSSGGSGSSSNNVAKPNTKPQTTQQQ
                     TDKAAAEKAAKDKAAKEKAEAERLAAEKAAKEKAEAERLAAEKAAKEKAEAERLAAEK
                     AAKEKAEAERLAAEKAAKEKAEAERLAAEKAAKEKAEAERLAAEKAAKEKAEAERLAA
                     EKAAKEKAEAERLAAEKEVDPIAIIKAKGIDDTNYGLKLGNSKETIEGKVTVRTQSYL
                     YNQPYSVVTAQAVQRNGWENGNYIDKVDGTVTVKGLKTEKLPTEGTATYSGKAFNHDG
                     AKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLIVLEEGTINNTQISSTVHGNNPLNLSG
                     KYTVEFFGKNAEEIAGEIQTKANLTGEVSNFGLAGTRGDIQK"
     gene            11965..13419
                     /locus_tag="PXD94_000342"
     CDS             11965..13419
                     /locus_tag="PXD94_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL6285245.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEIYWKLLTKLLEQAPEK"
     gene            complement(13494..14783)
                     /locus_tag="PXD94_000343"
     CDS             complement(13494..14783)
                     /locus_tag="PXD94_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6285246.1"
                     /translation="MDIRQKIAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSI
                     REELQLDGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYF
                     ATSAFEIGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGM
                     SAVLLQGEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVK
                     LGLGNGWELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPAL
                     LEEAGMPKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVF
                     AGNLGLAITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLGSIVSPT
                     LAGILLDAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
     gene            complement(14987..15322)
                     /locus_tag="PXD94_000344"
     CDS             complement(14987..15322)
                     /locus_tag="PXD94_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL6285247.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
     gene            complement(15312..15746)
                     /locus_tag="PXD94_000345"
     CDS             complement(15312..15746)
                     /locus_tag="PXD94_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL6285248.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            complement(15801..16856)
                     /gene="modC"
                     /locus_tag="PXD94_000346"
     CDS             complement(15801..16856)
                     /gene="modC"
                     /locus_tag="PXD94_000346"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL6285249.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVNQLPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVRKIETHLDRVDLAIQVENQQIWSTISLWSFNEL
                     AIQPEQEVYLQIKSVSL"
     gene            complement(16843..17535)
                     /gene="modB"
                     /locus_tag="PXD94_000347"
     CDS             complement(16843..17535)
                     /gene="modB"
                     /locus_tag="PXD94_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL6285250.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIMHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGAIPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
     gene            complement(17719..18474)
                     /gene="modA"
                     /locus_tag="PXD94_000348"
     CDS             complement(17719..18474)
                     /gene="modA"
                     /locus_tag="PXD94_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6285251.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKHRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWES
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVAVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
     gene            complement(18495..19256)
                     /locus_tag="PXD94_000349"
     CDS             complement(18495..19256)
                     /locus_tag="PXD94_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6285252.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            complement(19258..20166)
                     /locus_tag="PXD94_000350"
     CDS             complement(19258..20166)
                     /locus_tag="PXD94_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6285253.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            complement(20172..22229)
                     /gene="dnaX"
                     /locus_tag="PXD94_000351"
     CDS             complement(20172..22229)
                     /gene="dnaX"
                     /locus_tag="PXD94_000351"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL6285254.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAEKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            complement(22320..22859)
                     /gene="apt"
                     /locus_tag="PXD94_000352"
     CDS             complement(22320..22859)
                     /gene="apt"
                     /locus_tag="PXD94_000352"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL6285255.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGVKSFSLVSFEGH"
     gene            complement(22987..23937)
                     /gene="lpxM"
                     /locus_tag="PXD94_000353"
                     /gene_synonym="msbB"
     CDS             complement(22987..23937)
                     /gene="lpxM"
                     /locus_tag="PXD94_000353"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL6285256.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            complement(24378..25061)
                     /locus_tag="PXD94_000354"
     CDS             complement(24378..25061)
                     /locus_tag="PXD94_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL6285257.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
     gene            complement(25070..25702)
                     /gene="rsxG"
                     /locus_tag="PXD94_000355"
     CDS             complement(25070..25702)
                     /gene="rsxG"
                     /locus_tag="PXD94_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL6285258.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            complement(25702..26757)
                     /gene="rsxD"
                     /locus_tag="PXD94_000356"
     CDS             complement(25702..26757)
                     /gene="rsxD"
                     /locus_tag="PXD94_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL6285259.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMMLPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            complement(26803..27438)
                     /locus_tag="PXD94_000357"
     CDS             complement(26803..27438)
                     /locus_tag="PXD94_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL6285260.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            complement(27431..29725)
                     /gene="rsxC"
                     /locus_tag="PXD94_000358"
     CDS             complement(27431..29725)
                     /gene="rsxC"
                     /locus_tag="PXD94_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL6285261.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGANNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNAVIGVNNDLPLQSMNETVSDKSVGANNHSPETESEDPRKAAVAAAIAR
                     AKAKKQAQQAVEKPEDIANAVIGVNNDLPLQSMNETTSDKSVGANNHSPKTESEDPRK
                     AAVAAAIARAKAKKAAQQEKSNNV"
     gene            complement(29712..30329)
                     /gene="rsxB"
                     /locus_tag="PXD94_000359"
     CDS             complement(29712..30329)
                     /gene="rsxB"
                     /locus_tag="PXD94_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL6285262.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMA
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            complement(30329..30910)
                     /gene="rsxA"
                     /locus_tag="PXD94_000360"
     CDS             complement(30329..30910)
                     /gene="rsxA"
                     /locus_tag="PXD94_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL6285263.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
BASE COUNT         8659 a         7202 c         6753 g         8616 t
ORIGIN      
        1 agattagcga tacctagtac cccgagtgat attaatgcgt ataaaaactt tagatctaaa
       61 ctaaaccatc ttattgcttt ttaattagta tcaatgccct attatctact tgtatagggc
      121 atttcccttc atccttgacc caaatcaact ttccccttga tatgtggaaa accacaatac
      181 cttgccgttc cctctccgcc ttagaatacc tttgttattt ttaaatttgt gggggaaata
      241 tggatttaag caaacgttcc tttttgaaag agctttccgc cttgacggtg ggggcgagtt
      301 ttgtgccgtt gcaatcggcc caggcgttta tgccggcttg gcgtgagggc gatgagaaca
      361 aacgctatgc gatgctgatt gatttgcgta aatgtatcgg ctgtcaagcg tgtaccgtga
      421 gctgttcggt ggagaacgcc acgccgttgc acagtttccg caccacggtg cgtcaatatg
      481 aaatcaccaa cggcacgcag gtggtgaaca atgtggtgtt gcctcgtttg tgtaaccatt
      541 gcgatcaacc gccgtgtgtg ccggtctgcc cggtgcaggc aacctttcaa cgtaaagacg
      601 gtgtgatggt gattaacaat gagcagtgca tcggctgtgg ttactgcgtg caagcctgcc
      661 cgtatgatgc ccgttttatc aatgaagaga ccaaaaccgc cgataaatgc accttctgca
      721 ctcaccgttt ggaggtaggt ctgctgccgg cgtgcgtgga aagttgcgtg ggcggggcaa
      781 gggtgattgg cgatttgaac gacccgaaaa gtcaaatttc ccaactttat caagcccaca
      841 aagatgattt gaaagtcctg aaaccggagg ccggcaccgt gccacacgtt ttctatgtgg
      901 ggctagacga tgatgccttt gtgagcaaaa ttgacgggca accaatgctc tggacggggg
      961 aggcgtaaaa tgattcgtga agtattagtg gaaccgcaac atatcgtctg gttgccgtgg
     1021 attgtgcatt atttcttctt cgtaggcgtg gcggcaacgg cagtgtttac cgccgtgctg
     1081 tttgcaaaaa aatcactgaa accgaccgct tgcgagctgg cggcggtgac ggtagccttg
     1141 attggctcga ttgtcgcccc tgtggcattg actgccgacc tgcaccaacc gagccgtatt
     1201 ctgcatttct acaccgattt tgcgtggcga tctccaatgg cgtggggagc gattatcctg
     1261 cccctgttca gcgtggcggt agcgggttat tttgtgctgg cattggctca ccatacccgg
     1321 ccgaacctgc cgaaatggtt agcgtggcta cagttgccga tactgcaaaa tcaaggcttg
     1381 ctttgggcat tccgcctgtt tgccacactc accgctatcg gcattatcgg ctacaccgtg
     1441 cttgaaacct accaaaccgg cacaagaacc ttatggcaca gtgcgtggct gctgccgatt
     1501 atgctctttt ctgcgtgggc ggtggcattg gggcttaccc aagcggtctg caaaatgctg
     1561 ttccacaccc aaaccaacac ggggctatgg acaaaactct gcctgatttt aaccgcactt
     1621 tcgcttgtcg gactagcatt cagtagtcca caagccacac aagattttgc ccagctcttc
     1681 aatggctcaa tcaccgctta ccttgtcggc attttctggc taattgccct catttgcaat
     1741 ttttctgcca aaaaccaccg cttgcaatgg ctcggcgtgc ttgccctcat cgctttcggc
     1801 tggctactcc gctgggtact ggtgatccaa gtccaaacca ttgccaaaac caatgccttg
     1861 caaaatccat atcatttcga ttggacagcc gtcgatggtg gtttggggat tgtctcgatt
     1921 tttgggctaa cggtgctagt aagtatcgtg gtatggcaac tgattttaat aatttgtggg
     1981 ggaagaaaag gtgaacaata aacgcagaaa tctgttgaaa ggcgggctgg cacttggtgg
     2041 ggcggcggcg tttggggccg ggtattcgcc gaaggtgtcg gagattgcca aaggggtgat
     2101 gcacggcaca tcgggcgaga aaacccgtga tgcaattcac ggtaattcgc tcacgccgga
     2161 gtatcaggta aaagacggca aactgctcag caatcctgag caaattgtgt gcaatacaca
     2221 gtgtatgggc tgttggacgc agtgtggcat tcgggcgagg gtggatttga acaccaacca
     2281 agtgattcgc atttcgggca atccgtatca cccgctttcc tccgacaaaa ccctgccgtt
     2341 cggtatgccg attgccaagg cggaactgtt gctggcgggc gaaagtggca ttgaaaatcg
     2401 ctccaccgcc tgtgctagag gggcggcgtt cttggagggg gtgaacagcc cgtatcggat
     2461 tacccaaccg ttgaaacggg tcggaaaacg gggcgaaggc aagtggcaga ccatcagctt
     2521 tgagcagttg gtggaggaaa tcatcaatgg tggcaaccta tttggtgaag gcgaagtgga
     2581 gggcttgaag acgatccgca atctggtcga tccgattaac ccgaaacagc ctgatttggg
     2641 aatgaaagca aaccagcttt tagttacttt tgccggcccc gaaggtcgtc agccgatttt
     2701 gcaacgcttt gcccaaaaga gcttcggcac gatcaacttt tccaaccacg gggcttattg
     2761 tggcgctgcg taccgtgccg gttcgggtgc ctttatgaaa gattttgaca accaacaaca
     2821 cgccaagccg gactgggatc acgctgaatt tattctgttt atcggcacgt ctccggcaca
     2881 gtcgggcaat ccgtttaagc gtcaggctcg ccagcttgcc aaacgccgcc ctgatgacaa
     2941 tttcagctat gtggtggttt cccctcgttt ggagatgacc tcctccctcg ccacccaaaa
     3001 caaccattgg gtgccgatta agcccgaggg cgatttggcg ttggtaatgg gaatgttgcg
     3061 ttggattatc gaaaacgagc gtttcaatgc tgactacctc tcccgcccaa gtctggcagc
     3121 gatggaaaat gccggccacg tcagctatgc caatgcgtca catctgatta tttgcgaacc
     3181 gaaccacccg aaattcgggc aggcgttgcg gattagcgat ctgcaagatg tgcaactcga
     3241 ccccgaagag aaagtggaag aaaacctgat tgtggtaaaa gatcaggcaa gcggcgaact
     3301 catttcagca aaagattgct tacaagcggt catttttgtc gaacaaattg caaaactgct
     3361 tgatggctcg gaagtgttgg tgaaatcctc aatgcagttg ctgaaagagg cgacacaaga
     3421 gaaaaccctt gccgaatata gcgacatttg tggcgtgccg gtgcaaacca ttgaaaactt
     3481 agcgaaaaaa ttcacttcgc acggtcataa agccaatgcc atcactcacg gcggcacgat
     3541 gcacagcacc ggtttttata ccagttgggc gattttgttg ctgaatgcaa tggtcggtaa
     3601 cctcaacaaa aaaggcggaa tggcagtagc cgcaggcaaa ttcaaagatt tcggggacgg
     3661 gcctcgctac aatttagcga acttcccgaa tgaagtgaag ccaaaaggca cgaaccttgc
     3721 ccgcagtaaa aaggcgtacc aaaattcaag cgaatttaag cagaaagtga cgaagggcga
     3781 aaatccgtat ccggcacaag gggcgtggta tccgttcacc ggcgggcaaa ctaccgagat
     3841 gtttacctca atggtgcagg gctatccata ctttgcgaaa gcgtggatca accatatggc
     3901 gaaccccgtt tacggcttaa ccgctatgaa cccgattgcg ttggagaaat taaaagatcc
     3961 gaaaattcta ccgctctttg tctctattga tgcttttatg aacgaaacca ctgcactgac
     4021 ggactacatc gtgccggaca cccacaactt tgagagctgg ggcttttcaa cggcgtgggg
     4081 cggtgtgcct caacgcacca gcaccgcacg ccacccgatt gtgccgtcca aaaacgccaa
     4141 aactgccgaa ggcgacacca tctgtatgga gaatttcgtg attgcggtgg cgaaaaaaat
     4201 gggcttgccg ggctttggcg aaaatgcgat tgccgattta gaaggcaacc gctatccgct
     4261 ccaccgtacc gaagattact tcctacgggc ggcagcgaac attgcctttg ccggtgagaa
     4321 acctgcaccg gacgccagcg agcaagatct gctgctcacc ggcgtgcaac ggattatgcc
     4381 gaaaattgaa caaacgctaa aagccgaaga gcgattaaaa gtggcgaatg tgtattgcaa
     4441 aggcggacgt tttgccccac acgaaagtgc gtggaaagag gagaatatgg agttccagtg
     4501 gaaaaactgc ctgcaaattt ggaacgaaaa cgtgtataaa gcccagcacc acagcaacgg
     4561 tgaacactat tggggctgcc ctcgttatat gtcgccacgt tttgcggacg gctccacgct
     4621 ggagcagcac tatccgcagt cggaatgggg ctttaaactg atttcgttta aatcgaacat
     4681 tatgagcagt attaccgccc cactgttgcg tctgctctcg atcaagcctg agggcttagt
     4741 ggcgatgaac cgtttagatg cggaagaaaa aggcattaaa cacggcgatg tggtgaaact
     4801 cagcacccca accgccggct tgacggtgca gattgtggtg ttggacggta tcgcccgagg
     4861 cacaatcggc atcgaacacg gctacggtca taaacagatt ggggcaagtt cctacaccat
     4921 tgacggcgtg gaaattccag ccaacccgat gatcgccaaa gggattaacc tcaacgacct
     4981 cggcattatc gaccacacca aagcggtcaa atccccgtgg gtagattggg tctgtggctc
     5041 agcggtgaga aatggcattc cggcgaaggt agagaaatta gcctagccga acaagcggtt
     5101 ggttttgcag aaaaatgtgc aaaatcgacc gcttttattg atgtaaggga cactgttcct
     5161 tatgcaaacc agcattgcag agcgtaaaaa atcatactct gcattttaat ttattttgga
     5221 gaacctatga cttttgtcaa aaaaacagca ctgagcctga ttgcaacggc acttttcgct
     5281 acatcaagtt ttgccgctga gcagagccaa taccaacata ttcgtaatgc aacggcgaaa
     5341 attgaatatg ccggcaagac gtttttggtt gatccgtatt tagcggagaa agatcgctac
     5401 ttaccgccga atgcgaaaaa acgtaatccg accttggata tgactgtgag cattgatgaa
     5461 gtgttaaatg gcgtggatgc ggtgatcgta actcacactc accacgctca ctgggacgat
     5521 gtagcacaac aacgtgtgcc gaaaaatctg ccgatttttg tgcagaatgc cggtgatgcc
     5581 cgtttaatcc gttcacaagg ctttaccgat gtgcgtgtgg tcggcaaaga taccccgttt
     5641 gaaggcgtaa ccttaacccg aactcgtggc cagcacggca ccgatgcgat gtatgcgaac
     5701 ccgaaaattg ccgaaatggt cggcgatgcg atggacgtag tgatgaaagc ggaaggcaaa
     5761 ccgaccgtct atttggtggg agataccatt tggaaccacg aaatcgaaca ttcgttcaac
     5821 acttacaaac cagactttgt ggtgctgaat gccggctgtg ccaaactacc aaactttgaa
     5881 ggcagcccga ttatgtgtac cgaagatgtt tccaaagcac acaaaatcgc tccacaagcg
     5941 aaaatcttaa ctgtgcatat ggacgccctg ccacaaacct caatcagcag tgatcagatg
     6001 cgtgattatg tacaaaaaga gggattaaat cgtgtaactg tgccgaaaaa cggtgatgtg
     6061 attaagttct agtagctaaa ctaacattac caaaatggtt taagtagcaa taaatagcga
     6121 agcgggttaa gccatcaagc cttaacccac ttttaccaac catagtatat agttttagat
     6181 ataattatct tctgtattta cctgccattg cttggtaaac cgcattctcg ttggtcaaaa
     6241 tcgcatattt tgcatttaaa atcgccaact gagaattaag ctcggtattc agtgcggtaa
     6301 tccactcacg gaactccgac accccttgtt cataacggtt tttatagtat gaactgatac
     6361 gtttattttt cgcattggta tcttgaagga tttttaacga gcttctcgac tgcgtataac
     6421 tgtaataata acggtcaatt tcatttaagg ctgaggtaat tttttgctca tagtttaaac
     6481 gggcagtcac ataatccgtt tcagaaattt ttacgttatt tttaacccga ttccaatcta
     6541 agaacggtaa atccaattta attaagccgt tacccacagg gttatctgcc acattgccga
     6601 tattggttgc actaccggta atagaaccac cgatggttaa ggttgggaac cagcttttct
     6661 cggttgcggt taaggtttta aatgcacttt gtaagcgttg taacgagcct actacatcag
     6721 ggcgattagc aatggctgac actggcacat ttaaatctac cccttgcaat ttcacgctca
     6781 taatattcgg gtaacgaatg ttaagcggag tatttggctg aacatttaac aaattgcgta
     6841 atgtttgctc ggcaattttt tggttggttt tcagatcaat tacactattt ctggcgttta
     6901 agaccgcttg ttgagcctga tcaaccgcta aactatcaat cgcaccggca ttcattttgt
     6961 tagttaaaat acggctgatt tgctcataat ggcgaatgct ctcttcggta atacgaatcg
     7021 catcacgata gtaagctaac tggtagtaag tagtcacaac cgcattaata atagataaac
     7081 gggtagcttt taagtcttca atggtggctt tatgcgtcca ttctgccgca ctggcggtat
     7141 cggctaaacg tcgccataaa tccacagtat aacttaaatt aaatgcagct tgagctgcgg
     7201 ttgttgaaac accggtcgaa acattatttg aagaagccga acctaaacct ttagccgcag
     7261 aaacagaacc tgaaccacta aaggttggca ctaaatttgc ccctactaaa ttagcgttat
     7321 acaaggcttt attgactgca attccgcttt tggctaaatc aaggttattt ttgatggcta
     7381 aatcaaccac acggtttaat tccgcatccg tatagccttg ccaccattgc tcgttaattt
     7441 gatattgttt ggtaatttca tcatagcttt gataggtttg ctgtgcttgt tgcaaagtcc
     7501 cgtcattgct catattccct gtggtacaag ccgttacggt tagagccaac gccacagaaa
     7561 atgctaattt tgaaagtttc attacatttc cttttgaaaa taaagagggg aaaatgggcg
     7621 tattctaccc aaaaacagat tgcctttcca gttaaatgaa caactgttca tttaattatt
     7681 taacaaatca gtaatcaaat gctgatgacg aatttgttgc gaacctaagc tcaaacgctc
     7741 ggcaattaaa atcgctttta gctctgccaa tgcctgttgg aaatcatcat taataatcac
     7801 ataatcgaac tcattgtaat ggttcatttc cgacaccgcc tctgccatac gtttggcaat
     7861 ggtttcagcg gaatcttgcc cacgcccgat taaacgtttt tccaattctg tttttgacgg
     7921 tggcaaaata aaaacggttt tcacattcgg cactttttcc cgaatttgtc tagccccttg
     7981 ccagtcaata tccaaaaaga catcaatgcc ctgctctagg ctacgttcaa tcatcggcaa
     8041 tgatgtgccg taataattgc caaacacctc cgcccattct aaaaaatacc cttgctcaat
     8101 cagctgctta aactcatcgt gcgaagtgaa ataataatgc acgccgtcct gttctgccgg
     8161 acgaggctga cgggtagtgt gtgaaatcga aagctgtact tccgaacggg gtaaatccgc
     8221 caacaaggcg ttaattaagg aagattttcc tgctccactt ggggcagaaa taatataaag
     8281 attgcctaaa ctcattattt gtatatacct atttgatttt gggaataggg cgaatgatga
     8341 ttcgccccta catttcatag gaataaataa aaaataatgg acaactttca ttgtccatta
     8401 cacaaaatta tctacgtgcc aaaggctgaa cgaaggtaat gcctaaatcc caaggctgct
     8461 caatccacgt ttcttgtgga atatcaatca cataatcatc aaccaacggt gcaccgtgcg
     8521 gtttcgcaaa cacggttaca aaacgagctt tcgggtacat tctgcgaatt tcttttgccg
     8581 tgttgccggt atccactaaa tcatctacca caataaagcc ttcgccatcg gtatcggctg
     8641 cgtgtaacac atttaactca ccttggctat cgtgatcgta gctggcaatg cacactgttt
     8701 ctacgtggcg aatacccaac tcacgtgcta aaacggctgc cggaaacaag ccgccacggc
     8761 tcaccgcaat aatccctttc cactgtgtgg caggtaataa attttctgcc aacttgcgag
     8821 cgtgcatatg gaacatatcc cacgtcacga catatttttc atttgtgtat ttttcactca
     8881 tagaaacaac ctaatataaa attgggggct accccgatat aaaaaattgc actaggataa
     8941 cccgaaaatg cttttttcgc catcatttca tttatttttt tgtgccggca gctacgcaat
     9001 cgtatgctta gcacaaaaaa ccaacactaa aatagctttt ctaaagtgaa aaagacttcc
     9061 ttgttttggc gagagtaaaa atcagcaata ttgctgtcaa ttttttgata acggaaattc
     9121 agcttcggca caatgccctg ccaatgccaa gtgcggttcc aaaccgctaa attggcttga
     9181 tattccgtgt ctaaccgctt atagccaaac aaatagtgct tgtcggcaaa atatcgcttg
     9241 ccaaagccga tacttgcctg cgttgcccag cgttcgcctt gaaaaaccgc ccccactcga
     9301 gccagccact tattcgagct ttgtgctttt tcccgtgcaa tttcacgcac gccgtctaaa
     9361 tttgcaaaaa accgcccatt tttgaccgct tgccactgca caccgccact gagttggtgc
     9421 tgatagctgt tataacggct tgcggttagc tcctcaaaat accgtttttg caaatgggta
     9481 aaactccccg acaccaacca ttgcgaatta aggtaacgca aaccgttgag tgttgcccca
     9541 aattgaacat tataacggct actccccagc caattttgct caataaaagg cgaaaaagtc
     9601 agccgataag cgacggtgcg gtagctataa ttcggcgaaa cggatacgga ttgctcacgg
     9661 taatctcggt tatcccaata gtaaatcccg ctataattga gcgttaaacc gacaaaatga
     9721 ttgcctttca cgttgtgcaa tttactcaac cctaagccat aacgcacgcc ttttgctgat
     9781 tgtggcaaac tgtctgcatt tttgcggaaa atccgcccat ttacattcac aatcggcgaa
     9841 gatgaggcat tattcacgtt atctgtttgg gtatattgca gataaaaatc gggctgccac
     9901 gcctcatttt cagcaatttg gacttgagta ttgcgggcaa ttttagcaag ttccggcggt
     9961 aaatgggctt gaatagcctt aaattcctgc tctgccgcac gaaattgctg atcttctgcc
    10021 aaaatcaacg ccaaatcaaa acgagggtaa accaaactcg ggggcttagc taacagttgg
    10081 cgatagtgaa aaactgcggt ttgatgatct tttttcgcat aggcaagtgc ggcttgcgca
    10141 tagtgccaca acaacgggtc aaaattttca gtttgcggat aacggcttaa aagcggttca
    10201 agcgttgccc aatcttggct gagaatcgct tggttaattt gctgttcaac attctgattg
    10261 actgaatacg caggctgata agcggtcggt ttttgtgaat tttttacaac attttgctca
    10321 aatttaaccg cttgcggctg acgctcaaag gaaatcgggt cagtgttgtg ttcaatcacc
    10381 ttttctgccc atacaacggg gctgaacagc gaaaataaaa ggggatattt tttcataaag
    10441 ataaagaaaa ttggtagggg cggacctata tgttcgcctc tacgggattt atcactctct
    10501 ccccttgtgg gagagggtta ttagaagctg taaattgaaa tctaaactat tgtgtcttat
    10561 ggtatctaca agcaatatca agacaaatgc cgtttaattt ttcgttaaac ggcattgaac
    10621 gttagaaaag gttatttttg gatatcgcca cgagtgccgg caagtccaaa attactcact
    10681 tccccagtaa gatttgcttt tgtctgaatt tcaccggcaa tttcctctgc atttttaccg
    10741 aaaaattcga ctgtatattt tccagaaagg tttaaaggat tattgccatg tactgttgaa
    10801 gaaatctgag tattattaat cgtcccctct tctaatacga ttaagtttcc aaattctccc
    10861 tcaactctac ctgaaccggt acgcttagag aaattaacat tataggttag atcaccacca
    10921 ttaaaacctt tagctccatc gtggttaaat gcttttcctg aatatgttgc agtaccctct
    10981 gttggtaatt tctctgtttt caatccttta actgtaactg ttccatcaac tttatcaata
    11041 taattaccgt tttcccagcc atttctttga actgcttgtg ctgttactac agaatatggc
    11101 tgattatata aataactctg tgttctgact gtgactttac cctcaatagt ttctttacta
    11161 ttaccaagtt ttaagccgta atttgtatca tcaatacctt ttgctttaat aatagctata
    11221 ggatctactt ctttttctgc tgctaaacgc tccgcttccg ctttctcttt cgccgctttt
    11281 tccgccgcta aacgttctgc ttcggctttt tctttcgctg ctttttccgc cgctaaacgc
    11341 tccgcttccg ctttctcttt cgccgctttt tctgccgcta aacgttctgc ttccgctttc
    11401 tctttcgccg ctttttccgc cgctaaacgt tctgcttcgg ctttttcttt cgctgctttt
    11461 tccgccgcta aacgttccgc ttcggctttt tctttcgctg ctttttccgc cgctaaacgt
    11521 tctgcttccg ctttctcttt agctgctttt tctgctgcta aacgttctgc ttctgctttc
    11581 tctttcgctg ccttgtcttt tgctgctttt tctgctgccg ctttatcggt ttgttgctga
    11641 gtagtttgcg gtttagtatt cggttttgcc acattgtttg agctactgcc cgaaccacca
    11701 ctactacacg ccgttaaacc caataaaatc aaactggcta aagttacttt tttgatagac
    11761 ataaaatgtt ccttttgtaa gataaacaaa aatagatgtg tataaaacac acgcatattg
    11821 tacaaaaaaa aaacaactcg tcatcaagta ctttttaaga tttttttgtt aaatttctcc
    11881 taaatattct acaatgtcag aggtctatat cagcttgaat acaagcggtt attttttctt
    11941 aaaattttac aaaaggggct tattatgtct gaaatctcaa cgctcgcacc gcagctactt
    12001 tggcagtggt ttgataaagc ctgtgctatt ccgcacccgt cttaccacga aaatgaactg
    12061 gctgaattta ttgtgaattg ggcaaaatcg aaaaatttct ttgttgaacg tgatgaagtg
    12121 ggtaatgtgt taatccgtaa accggcaact aaaggtatgg aaaaccgcca aactgtcgct
    12181 ctgcaagccc atcttgatat ggtgccacaa gccaataccg gcacggttca cgatttcact
    12241 aaagatccga ttcagcctta tattgacggc gaatgggtaa aagccaaagg cacaacattg
    12301 ggggcagata acggcattgg attggcttcg tgcttggcgg tgttggatag cgatgattta
    12361 gcccacccaa ccattgaagt gttgctcaca atgaccgaag aagccggtat ggaaggggcg
    12421 attggtttac gcccgaactg gctaaccgcc gatattatga ttaacaccga caccgaagaa
    12481 aacggggaaa tttacatcgg ctgtgcaggt ggggaaaatg tgaatgtgac actgccaatt
    12541 ttcttggtgc ctcatcagca agatagtgca gtaaccgtta cgcttaaagg cctaaacggc
    12601 gggcattcgg gctgtgatat tcacaccacc cgagcgaatg cgattaaagt gttcgcccgc
    12661 attttggaac aagcccacaa cgaagtggaa ttccaaattg cagaaattca aggcggttcg
    12721 gtgcggaatg ccattccacg agaagcccac gcaaccttgg ttttctctgc aaaaaatcag
    12781 caaaaattaa ccgcttgttt aagccaaacc gccgaacaag taaaagcaga actcaaaatt
    12841 gccgagccaa acttccaatt tttaattgaa gaagctcaac tgcctgagca agcctttgac
    12901 cttgccagca ctgctcgagt aatccatttc ttaaatgtga tgccaaatgg tgtggtgcgt
    12961 aatagcgatg tggtggaaaa tgtggtggaa acctcactca gtattggtgt gcttaacacc
    13021 gaagcgagtg aggtgaaagc gaccattctt gcccgctcat taattgaggg cggaaaagag
    13081 gaaattcgca gtaaaatccg ctcattaacc gcattagtgg gagcagaagt ggaattttcg
    13141 ggcaactacc cgggctggga gccaaaccca aattccaaaa tcacgccatt aaccaaagcg
    13201 atttatgatg aaattttagg ttatgaatct caaattaaag tgattcacgc agggctagaa
    13261 tgtggcttaa tcaacaaaat ctacccgaat atggattttg tctcgattgg accaactatt
    13321 ctcaacgccc attctccgga cgaaaaagtg catattccgg cagtggagat ttattggaaa
    13381 ctcctaacca aactacttga acaagcacct gagaaataaa tttaatcaaa aaaattatat
    13441 aatcttgccc cgtaattaat tgaataaaac ctcaattaat tacggggtag ctcttatttt
    13501 ctctgtttta aaaaatgtac cgtgaaagca gcaattaata agacgactgc tgcacctaaa
    13561 taaagctcgt tctttttcca gccggcgtct aataaaatac ctgcaagagt tggggaaaca
    13621 attgagccta aacgccctac accgatggcg gtgcctacgc ccgtgctgcg gaaatccggt
    13681 gcgtataggg ttgggttaat ggtataaagc ccggtaatgc agccgttaat taatccgcca
    13741 accaaaatgg ctagtgttat cgcaagccct aaattgccgg caaacacaaa ggcaaccaca
    13801 gcagatgcag ccagaatact gaatgcctgc aaaatatgtt ttgccgccca gcgactgact
    13861 aaaaagccga aaattaacga gccgattgta ccaccaactg aaattgccat tcccacagtt
    13921 tggctttggg ttttaggcat tccggcttct tctaatagtg ccggagtcca agagctgata
    13981 aagtaaaatg aggctgaaat tgcaataaag gcaagccaaa ttagcaacgt attgcggcga
    14041 tattcaggct cgaataaacg ggtaattgcc actttgcttt tactttttgc ctcaatttca
    14101 ggaagttccc agccgtttcc taagcctaat ttaacggcaa tttgatttaa acgagcttta
    14161 gcattcaccg ggtgttttga aattaaaaat tccaccgatt cgggcaatac aaagaataat
    14221 aaaacgaata ccgcaccggt taaaatcgac ccaacgagga aaaccgcacg ccagccatat
    14281 tctccttgca ataacaccgc agacattccg cccagcattg caccaatccc aaagcctgag
    14341 gcataaattg caatcgctaa gccacgccat ttttggctgg agtattcact cactagcaca
    14401 ttagtgccgg ttaaaatggc ggctacgcct aaaccggtta aaacacgcca tacaccaatt
    14461 tcaaacgcag aagtagcgaa ataagttagg agcattccta acgtggctaa gcctgtgccg
    14521 attaaaagca acgggcggcg accaaattta tcagcaagtg gtgaaagaaa gagtgagcct
    14581 atcgccatac cgaataaacc ggcactcatt aagctgccga tggctgcacc gtctaactgc
    14641 aattcttctc ggatgctttt ggcggtaaag gcgagtgcca ttaaatcaaa gccgtccagc
    14701 atattaagta acaccgtaag cccaaccact aaccattgga atgaagtcat tttgttatga
    14761 gcaatttttt gacgaatatc cataagctcc ctctgcatca aaattgataa aattttattt
    14821 agagtgaact atagaaagtt gattaaatat aatcaaactc aaaatttaca taaaatttat
    14881 gaaagatttt catatttaat caaaggaggg aatttatagc aggaatggcg agggagaaaa
    14941 tagatagggc gaataattat tcacccctag attgaatagg agaaagctat ttcttctccg
    15001 cttgctcagc cgctcttttg cgtctgattt ctttcggatc agcaattaac gggcgataga
    15061 tctcaattct gtcgccttct tccaccgttt cattcagttt tgccgggcgg ctaaaaatac
    15121 cgactttgtt ttcacgcaag tcaatttcgg tatatttttc caacacgccg gagagcaaaa
    15181 taatagtttg aatggtcgcg ttttgcggta cttgcagttt tttcagaaaa tatttatcag
    15241 ggtaagcata aagcacctca acattaattt tctgttcttc acttttttgt tcttctggat
    15301 tttgacgttg ttcagacacc atacacctct tttgcacgtt gtttgaaagc atttaccatt
    15361 tttaaagtta attcgttgaa caccttgccg aacaccattg ctacaattgg gctggaaaat
    15421 tcaaaattta attgcagggc aattttgcaa ctttgctcat caaagggggt gaatgtccaa
    15481 gctccgtgta aatggcggaa aggcccttca attaatgcca tctcgatttt atagggcggc
    15541 gtcattttat tgtgggtact gaaagtttgg ctaatgccta atttctgaat gtgtaactct
    15601 gcctttaatt cattttcgcc ggtgctaagg gttttggtac tcacgcagcc ggataaaaat
    15661 tcggggtatt tttcatactg attaacaagt tgatacattt gctcggcact ataaggcacg
    15721 agggcagatt gattaacgat tggcataatg tctcttttgg gaattaacaa gcggtcatat
    15781 tttagcgtaa atttgcaagc ctataaagaa acgcttttga tttgaagata gacctcttgc
    15841 tccggttgaa tcgctaattc attaaaagac caaaggctga tcgtactcca aatttgctga
    15901 ttttcgactt gaattgccag atcaacccga tctaaatgcg tttctatttt ccgaacattg
    15961 ccttttaaca cattacgaat tgagctttga gtcggttttt gtaagctgat tgaaacctct
    16021 ttgctggcaa tagttaccct tagcttctcg cctatttgat ggcgggggag ttggttcacc
    16081 cataatcgct gtgcgttcaa gctcaagcca agcatattat agtggggaag tgtttcttcc
    16141 accggtaatt caagcaggct aattcgctga tgatttggct gccaagcgga aaatgccgag
    16201 ctgtgccaaa ccgctgaaag ggtatcaaat gcggttactt tgccgttttc caacagcaca
    16261 agatgatctg ccaatcgaac tacttcctct aaactgtggc tgacatacag aatcggtatt
    16321 tcaagccgag cggataattt atctaaatag tccagtagct cttgcttacg agggagatcg
    16381 agggcagata atggttcgtc catcagcaaa atattcggtg agcttaataa cgcccgccca
    16441 atcgccacac gttgtttttc gccaccggaa aggcttgccg gatagcgatt aagcaaaggg
    16501 gcaatgccta acagttccac aatttgcaaa aaatccgcag aatccgaccg cttgtagcca
    16561 tattttaagt ttttttccac cgaataatga gggaaaaggc gatgctcttg aaacacatag
    16621 ccaatgttgc gtttttcagg tggtaaattg atgccgtttt ggctgtcaaa cagcacctga
    16681 ccgtttaatt cgataaagcc ggcttgagcg gttgaaagcc ccgcaattaa attaatcagg
    16741 ctggatttac cggcaccgga acgcccgaaa atagcggtga ttcctttatt cggcaaatcg
    16801 agattgacat tgagcgtcag attgccgagt tgctgaagaa gtttaagttt gagcatagcg
    16861 tttttgcctt tctgccagcc attccgaaca gaacagggca atgagtgaaa tcagaatcgc
    16921 caccaagcac aaacgggcgg cagccatttc gccatcgggg gtttcaataa aggtgaaaag
    16981 ggcggcagga atggtttggg tttggttggg aatgttggag acaaaggtga tggttgcccc
    17041 aaactccccc aaagagcggg caaaacccag cacgccaccg gcaataatgc ctgaaaaaga
    17101 gagcggtaaa gtgagcgtga aaaacacttt taacggtatt gctccaagcg ttctggcggc
    17161 ttgctccaat ttaggatcaa ctgcctcaaa ggctaaacgg atagaacgca ccattagcgg
    17221 aaacgccatc accattgagg caagcactgc tcctttccac gaaaaactca agctgaaatg
    17281 aaaccattcc cacaaatatt gcccgattac ccctcgtttc gccatcgaaa taagcaataa
    17341 atagccaatg acaaccggcg gtaacaccag agggagatgc ataataccat tgagcagatt
    17401 ttttccccaa aactgtttgc gggcaagcag ccacgccacc cacacggcaa gcggtaatgc
    17461 aagcactaca gcaatggagg caacttttag gcttagaaca atcgcattga gttcttgtgg
    17521 ggtgaaatta agcataatct aaaactattt ggttataagc ttaccctcgc cccttgtggg
    17581 agagggatag atttttcttc gttcagaaga aaaatcaggg agagggggaa tattttaaac
    17641 gctatttgcc ctctctctga aaaatatgtc tgaacgacat atttttctgt ctctctccca
    17701 caaggggaga gagtaaattt aatttacagg ataaaaccca gctgcctcaa atttcgcttt
    17761 tgctttcggg gttttgagaa aatctaaaaa tgtggcggca gaggcttttt cactgactgc
    17821 cgcagcaggg taaacaattt cgccgtagct ttccggtggg aaaacggcaa ccactttgac
    17881 tttattcgac actttagcat cggtggaata gacaatccca agtggggatt cgcctcgctc
    17941 gacaaaagag agggcatctc gtacattttt tgccctcgct aaacgaggtt caacactttc
    18001 ccataattgg taatgactaa gggctttttt ggcatatttg ccggcaggaa cgtggtttgg
    18061 gtctcccacc gataaatagc tgtttgctaa ttctttggca aaatcgaccg cttgtatcgc
    18121 ttttggctca atgttacttt cggtaggtgc aattaatact aactcgtttc tgactaaatt
    18181 gcggcgatgt ttaactttct caggttgttt ttcggcaaca taatccatcc acttttggtc
    18241 ggcggaaata aaaatatctg ccggtgcatc ttgctcaatc tgctttgcta ataccgatga
    18301 tgaagcgaag gagaacgtga ttttatcctg tggataggct ttttcaaaat cctgctgaat
    18361 atcttgtaac acatttgtca ttgaagcggc agcaaacacg gtaatctctt gtgaaaaggc
    18421 tgtgcctgat aacgttaatg ccattgtaat tgctagtttt tttaatgctt tcattttcat
    18481 aacctcacaa ttagttaaga acgcagccca acccctcgtt gaattaagga ataggcaata
    18541 atatacaagc cgatgcaaag tgaagtaagc actgcaaaag tataacctaa cggcacatcg
    18601 ctgataccga gaaagccata tcttaagccg ttaatcatat aaacgatcgg gttgaattta
    18661 gacaccgttt gccaaaattc aggcagtaat gaaatagagt aaaatacgcc gcctaagtaa
    18721 gttaagggag taagcacaaa ggttggaatc agccccacat catcaaagga gcgggcgaac
    18781 accgcattaa ttacgccacc taacgcaaag gcaatgattg tcagcaacag ggttaagacg
    18841 ataatcgcaa ccgagtagat ttcaaacgag acaaacacca ttgccacgca cattactaag
    18901 gttgcggcta aacctgcacg caaaatactg ccggtgacat aaccccaaat aatattatgg
    18961 gtagagagtg gcgaaatcag catctcttca aaatctcggg taaatttact caagaaaaat
    19021 gaagaggcgg tattcacata agaggcggtg atggcggtca tcataattaa gccgggtgca
    19081 ataaattggg tgtaacccac gccattcatt tccccgattc gcccaccaat cagcgtgccg
    19141 aagattaaaa aatagagcgt ggtagtaatc accggtggca ctaaagtttg tcgccaaatc
    19201 cgcaaaatgc ggcggcactc tttgtaagct aaggtataaa atccgattaa attcatttta
    19261 ctgagccata ttgataaata gttcttccaa gcggtttgat ttgtttcgca tacttagcac
    19321 ctcagcccct tgggcggaaa tttgcgaaaa cagattggtt agcccttgct ggcgttttac
    19381 attcacttca agggtgtttt catcaagcca tttatgcggg taattttgaa tcacaagcgg
    19441 ttgattttgc tcagaatttt gcaaatctaa cacaaatacc tcactttcca atttggcaag
    19501 caaggctttc attgaggtgt tttcaattaa ttcgccgtgt tgaataatgc cgatgtgacg
    19561 gcatagggtt tccgcctctt ctaaatagtg ggtagtgaga ataatagttg tgccttgatt
    19621 gtttagatcc cgcaagaaat cccacaatga gcggcggagt tcaatatcca cccctgcggt
    19681 aggttcatct aaaatcagta gttttggatt gtgcattaag gctcgggcaa tcatcacccg
    19741 acgtttcatt ccaccagaaa gctcacgggt gaggtgggtg cgtttttccc ataggtcgag
    19801 caatttcagc cacttttcgg cacgtactaa ggcttcactg cgaggaatgc cgtaaaaccc
    19861 ggcttgttgc actagcacat caatcacttt ttcaaactga ttgaaattaa actcttgtgg
    19921 cactaagccg atttgatgct ttaattcgac tttttgggtg tctaaatcat agccaaacac
    19981 tttcacgctg ccgctggttt tatttaccag tgagctgata atgccaatag tagtagattt
    20041 acctgccccg ttatgcccaa gtagggcata gaaatcacct tgctcgacca caagattaat
    20101 gcctttaacg gcttttacac ctgttgggta ggtttttacc aaatcaatta attctaatgc
    20161 tttcatttgt tttactcagt ttccgccacc gctcgaatgg tcgattcatc aatttgtgca
    20221 tcaaaagcgt tgcgaagtaa cattaatttt tcatcgttaa gcagagctga ttttgctgcc
    20281 tcagttaatt gtttgaaaat ggctcgccga atttcaagcg gagtttgctg ttcgctattg
    20341 tcaccaatgg tcaattcgta tgaatagcct ttttcggcaa gggcagcttc taaattttta
    20401 cgtgattccg gattatctaa gtgagccata ttcggcttta acaccaatgc gaggtgctgt
    20461 tcattttgtt ccgctaaata gctatttaag gcgatctgac gggaaaatcc gcctaaattg
    20521 agtgagttgg cgatgtcaca ccatttatcc tgctgacaag ataaatgaat ggtttttgcc
    20581 actaattcag gggtgcgttc ttgcaaaatc gcttgtttaa tatcagaagg tttggcactg
    20641 tctgcttgat tttctaattc aggattgagc cacgtccagc gataatcttc atcttccaca
    20701 cttttagggg cggattcggt tttctttgaa acttgcagta cggctttgtg ctgattttcc
    20761 gccaagctga taaaccgctc ttgcaatgaa gcggtctgat ttgccgattt ttttgcaata
    20821 acttgagggg cttttagctc aggctttttt ttttcggttt gagctaaatt tgcctgctgt
    20881 gcttgtagct ttttgcgggc ttcgagggct gccattgccg gtgaaagagt cgcaggagca
    20941 gacggcgaaa tagcggtatc tactgcctgt ggcggttgag caatgctttc aaccgctgtg
    21001 gtagtattgg caacagtaac cgttttttgc tgattttgct caaaattttc tcttagctgg
    21061 ctaatttgat tttgtggttt tgctgtcgta accgctgaaa ccgaatggtt tggttgtgta
    21121 accttttcca ctgcaactgt tgctattttt tctgcctgtt tagggtggaa agccaacgca
    21181 cgcaaaatcg ccatttcaac accggcacgt tgctccggtg caaaaggcaa ctctttttta
    21241 ctgttgagga ttaattgata gaaaaattgc acgtcttcgg gcggaatttg ttttgccaga
    21301 aaatgcacta atgtttcttc ggttgtaggt gttttcacta attgcaacat tgcaattttg
    21361 tgtaacactt ctgccacatc gcttaataat tgttgccaat caacgccttt cgctgcaatt
    21421 tgctcgatga tcgccattgc tttttcaccg tcggcttggg cggttgcttg cactaattct
    21481 agtggttgat tatcgtcaat caaccctagc atttggctga ctatcgcaag cgtaatattg
    21541 gcattactca ttgcaatggc ttgatcggtg aggctgagag agtctcggat actaccttgt
    21601 gctgcttttg ccaacttttc tagtgccaaa ggctcaaacg gaatgttctc tgcatttaga
    21661 ataaattcaa gatgacgggc aatttgcgat tgctctaatg cccgcagatg aaattgcata
    21721 cagcgagaga ggatcgtaat cggcagtttt tgcggatcag tggtagccag caagaatttg
    21781 acatattcag gcggctcttc aagggttttt agtaaggcgt tgaaactatg acgagagagc
    21841 atatgcactt cgtcaattaa ataaacctta aaacgcccaa cggtgggttt gtattgaacg
    21901 ttatcaagta gctcacgggt gtcttccact ttagtacgag aagcagcatc aatttcaatg
    21961 agatcgataa aatgcccttc ttcaatcgct ttacaattcg cacattcacc gcaaggctcg
    22021 gcggtgatcc cgtttacaca gtttaagcct ttggcgaata aacgggcaat agaggtttta
    22081 cccacaccac gagtgcctga aaacaggtaa gcgtggtgca gtctatcttc tcttaagctg
    22141 ttttcaagag cagataaaac gtgttgctga ccaactactt ccgagaagcg ttgcggtctc
    22201 catttacggg ctaaaacttg ataactcatt gatttttcta ttcaatttgg aaatcacaag
    22261 cggtcatctt tccgaaaaat tttgcaaaat tttagcaaaa agccaccgct tgttccccat
    22321 taatgacctt caaagctgac taggctaaag gatttaaccc cttctttttc caaacgctct
    22381 ttaccaccta actcaggcag ccaaatcaca aaggcagcgt gttctacgtt accacctaag
    22441 cgacgaatta gttttgcagt tgcatcaata gtaccaccgg ttgcgagtaa atcgtcgatg
    22501 accaatacat tatcattagc actcactgcg tctttatgaa tttctagcgt gtcttcacca
    22561 tattcaagcg tgtaagattg cgaaatcact tcacgtggta attttttcgg tttacgaacc
    22621 aacacaaaag gcacgcctaa cgctaatgca acaggggcac caaaaataaa cccgcgagat
    22681 tctgtgccga caattttagt aatacctttg tctttaaatt ctgcaacgat ggcatcaatg
    22741 gtggcttgga atgcagttgg cacttctaaa agtgaagtga tatcacggaa gataatccct
    22801 gctttcggat aatctggaat ggatttgata gaagatttaa ttaaatctaa ttggttcatt
    22861 gtttgaccta cgtgtaataa aaaaataact ggaaaactct atagaaaaac agcagaaatt
    22921 tcaaggagaa attgataacc ttacgtttga tagtacagct tacttttcag tttccttttc
    22981 cctatattat agataaatat cttccctgtt tcgtctggtt ttaagcaaac gtaaaatcca
    23041 aacatactgc tcggggtttt tctccacaaa atattcaatt actcggttca tttctcgggc
    23101 ggattgaatt gggtctcctg aaaattcaag cggtggcaaa atttccattt catattttgc
    23161 tttttccgca ttataaatcg agaacattgg gatcacttcc gcatttgcca ctcttgccat
    23221 tttatttaag cccggcaaag tggctttttc ggtggcaaaa aagtcggcat acacacttag
    23281 ctcttcgccg aaatcttcat cgggcagaaa ataaccaaca tcacctttac gcacatcggc
    23341 taaaaacgct ttaatcccat tttgacgagt gtgcattcgc ccacctaaac gctcacgggt
    23401 aatattccac agccagtcca ccaacgcatt acggtgtgga ttgtacattg atgtcatcgg
    23461 ataaccgagc gagaacatcg ccacccccgc catatcaatc gaccaagtat gtggcactaa
    23521 cataatcacg tttttaccgc tctctttggc tttcaccaca ttttcaaagc cggtaaattc
    23581 acaccgtttt tgtaaataag aggccgggcg gattgcagtt tcgccaatcg ccaacatggt
    23641 ttgtgctacc gtgataaaca ttttctcgac cacttcttca cgctttttct cactccaatc
    23701 gggaaaacag taacgcaaat tcactttagc acggtggtag ccttttttat ttttctgctt
    23761 gagatagcga tacgccatct tacccacaaa agcggataat ttatccctaa ttcgcaccgg
    23821 cacataggct aaaatcacta atcctaatac acctaaccat acgccccaat atttcgggtg
    23881 tagaaaagac cataggaatt tgtgcgtata gccgtcttgg gcggtaattc gtgtcattct
    23941 ataattcctc attatttgtc actttctttg cttcgccaaa gaaagtaacc aaagaaaggc
    24001 aaccctactt caccgctatc ttcgcttggt ttgaatttgc ttaacgaaaa aattccaaac
    24061 tcgccgcttc gctgctcaaa caaggaattt ttcctacaaa ttcaaagtcg ttcgggcggt
    24121 tcagaaggga acccggcaga gcatcatttt gcaaaatttt ccaaaaaaat caccgcttgt
    24181 atggtcggat cgggttccct tctgagttgc ccgaacgact tgaaattttt aggaaaataa
    24241 ctcgagcgag agcgaagcga acatcagggt tatttttcgt taagaaaatt tcaaccaagt
    24301 gaggatagca acaaagcagg gtgtgctttc ttttggttac ttttctttgc acaagcaaag
    24361 aaaagtgaca taacacatca tctcttatca ataatattct tcaccgccaa tatcacccca
    24421 agtccaataa aagcccccgg aggtaaaatt gccagtagta aacctgaatc aatatgcaat
    24481 acatcaatgc gtagggcttt tgcccaatcg ccaaggagta gatctaagcc tgaaaacagc
    24541 gtgccgttgc caataatttc acgcaacgcg cctagcacca caaggctaaa agtcattccc
    24601 agccccattg aaaagccgtc aaaagccgag aatttgacct cattttttga ggcataggct
    24661 tctgctcgtc caatcacgat acagttggtt acaatcagcg gaataaaaat cccgagcgat
    24721 tggtaaacag ggaaagcaaa agcattcatt aaaagctgta ctgccgttac caccgttgca
    24781 attaccatca cataaatcgg aatccgaata tcgtgtggag tgattttccg aaacagggaa
    24841 accgtgatat tggtgcaaat tagcacaaac attgttgcca agcctagtcc aagtgcattg
    24901 gttacgctgt ttgaaaccgc cagtagtggg caaagcccta ataattgcac caaagcacca
    24961 ttatttttcc acacaccatc agcgaaaatc gttttccaaa cgctggattg tttcggggca
    25021 acgtcaatat tcgtcacagg gatttgttgt tctctttcca tttctccccc tatttgcacg
    25081 ctttaaattg attaaacatt gtcgggtttt gagccagctc tgtcaccacc catttcgcac
    25141 tttggcggat atgattgacc accgcacgag gtgtaatagt tgcaccggta aattggtcga
    25201 acttgccgcc atcttttttt acattccaat ccgcttcgtt ttctaaacta aaaagttgat
    25261 tggtaaaaga taagatccaa tccgacactc gggtctcaat tttatctccc agccccggag
    25321 tttctttatg ctcaagggtg cgaacgccta aaatgttgcc gttcggctca atccccatca
    25381 atagcacaat atcgccggaa tagccgtccg gagccgttcc ttgaactagg taagcggtca
    25441 gattttccga tttttttgca acataaatac ggttcaaata aggggcattt tcgagctgaa
    25501 ccagtttgca agtcgccaac acatcattat caaaatggct tttcggcaca acctcttcca
    25561 ataatttgcg ttgttgcatt gcggttacat catcaatctt gcctttggta agcaggtaca
    25621 caccggttga aacagcggtg caaagcaagg caataaagcc taaaattaag gcgtaattta
    25681 aggtaatttt tgaggtgttc attatctgtt atccaatttt gtgccgtata atctcggctg
    25741 cgtataatgg tcgattaacg gtacgcaaat gtttgccaat aacactgaaa atgcaatcgc
    25801 atcagggtaa tttccgtagt agcggattag ataaaccaat aatcccacca agccaccgaa
    25861 aatcagtttt cctcttgggg taatggaggc tgttaccggg tcggtcgcaa taaaaaatgc
    25921 cccgaacatc atcgcaccgc taaagaaatg gctcacaaaa tgcaagtgcg attgttggct
    25981 aaacagctcg gtgaatccac taaaaaaggc aaaagttgcc aacattgcta ccggaatttg
    26041 ccaatggata atccgtttat aaatcaacac caaaccgccg aataagaacg caaggttcag
    26101 ttgtagccaa ccggtcgcaa acagcccgtt gaaaatcggc gtattcatta ccgcgtcaaa
    26161 acctgatttt gcaaggctgc ttttcatact atcaagcggg gtagcttgtg caatgccatc
    26221 aacactatta actagctgat gaatactaaa accatcgcta gttaccccgt aaaaaatcaa
    26281 cgaaatcgca tcacgaaatg tcggtggttc acttaataac tcaatcggca ctatccacgc
    26341 cgtcatttgc accgggaaag aaaccaacaa caacgcatag ccaaccattg ccgggttaaa
    26401 tagattctgc cccaagccac cataactgtg ttttgccaat aataacgcca caatcacgcc
    26461 aatcaacaca agccaataag gagcataagg cggaatcgac atcgccaaaa tcaaggcggt
    26521 taaaatacct gataagtcag ccaaatagaa agcggttgtt tttttgcgta attttgcaat
    26581 cccgatttca atcacaatcg caaggctaac ggcaagcaac gtttgaatca gcacaccata
    26641 gccgaaataa taaagctgaa ccccaaaagc aggtaacatc atcgcaatca cccacagcat
    26701 aaaattcgca gtgagattgc tcgaatgagt gtggggggaa ctgaccattt taaacatcta
    26761 taaaaccttt ttagtaaaaa attgcaaaaa aatgaccgct tattaaagtc tcggcaaatt
    26821 caaattaaag cgtattgcca gcaggcggat aataattacg gtgaacacgg tggcaaggta
    26881 aacccaatcg ctttcgagcc caactgagtt tgcagccaca aaaatcacac caccgataag
    26941 gctggcggtc acatacactt ctttttgcaa aatgatcggc acttcgttgc gtaaaatatc
    27001 acggattaag ccaccaaaac agcctgtcac accgcccaga gccacacaga tcagtgggtg
    27061 caagccgaaa tccaagcctt tttgcacgcc aatcacggta aatacgccaa gcccgatggc
    27121 atcaaacacc agcaagccgg tttgccaact tttgcgggaa atttggttgc ggaaaatagc
    27181 ggtaatcacc actgcaaggg taatcatcag cacatagatc ggttgtttca tccaaaacac
    27241 cggcgtggag ccaatcatca tatcccttag cgtaccgccc ccgaccccgg tcacaaacgc
    27301 taaaataaac ataccgaaaa tgtccatttt gtgctggcgg gcagcaattg cccccgaaat
    27361 cgcaaacacg atagtgccga ttaagtcctg aataaacagg aacgaccacg gcaatgccgg
    27421 aattagctcg ctaaacattg ttgcttttct cctgctgagc ggcttttttc gctttagctc
    27481 tggcgatggc ggccgcaacg gcggctttgc gtgggtcttc gctttccgtt ttgggcgaat
    27541 ggttattcgc ccctacagat ttatccgatg tagtttcgtt cattgattgt aggggcaaat
    27601 cattatttac cccaatcaca gcattggcaa tatcttccgg tttttcgacc gcttgttgag
    27661 cttgtttttt cgctttagct ctagcgatag ctgctgcaac ggcggctttg cgtgggtctt
    27721 cgctttctgt ttcgggcgaa tggttattcg cccctacaga tttatccgat accgtttcgt
    27781 tcattgattg taggggcaaa tcattattta ccccaatcac ggcattggta atattttctg
    27841 atttttcgac cgcttgctgt gcctgttttt tggctttggc tcgggcaagg gcagccgcaa
    27901 cggcggcttt gcgtgggtct tcgctttctg cttcgggcga atagttattc gcccctacag
    27961 atttatccga tgcagtttcg ttcattgatt gtagaggcaa atcattattt gccccaatca
    28021 aggcatttgt aacattttcc gatttttcga ccgcttgttc tgcctgtttg gctaaacgtc
    28081 tggctctgcg ttgtgccatt aaatcgctgt tatcaggctc gccgttaggt tttacctgta
    28141 cttctgccgg tatttcttgg actgtttcaa ccgctttttt cgctttcagg cgttcaagag
    28201 ccgcttttac aggatcaaca ccacctgcat ttgccacttc ttcacggcgt ttttccgccg
    28261 cttggctaat gcgagcagtt ctggcatctc gttctttttg taagcgagct tctctcgcct
    28321 caaagcggat tttagcctct tccgctttgc gggctttttc ctcaatttcc tcgatttttg
    28381 ctttttcttg gcggaaatat tggattaacg gaatgtagct tgggcaaacg taagcacaaa
    28441 cgccacactc aatgcaggca tctaaatggt attctttcga tttatcgtga tcttctgcac
    28501 gggcaaacca ataaagttgc tgtggcaata accccaccgg gcaagcatcg gaacagcttg
    28561 aacagcggat acaactacgc tccggttcag gtggtgcata ttcaaaatga tcaggggcaa
    28621 tcaagcagtt cgcagttttg gtaatcgggg cttgcaatgc aggcaaaata aagcccatca
    28681 tcggtccgcc taaaaacacc ggaaagcgtt tatcggcttg ataatctacc tgctccaaca
    28741 agtgctgaat tggcgtgcct aatcttgccc acacgttccc cttgttgcga attttatcgc
    28801 ctgtcagcgt cactacccgt tcaatcaaag gttcatcatc aataattgcc cgtttaaccg
    28861 caaacgccgt gccgacattg tgcatcacaa tgcccatttc aatagtgcgt ttgccagccg
    28921 gaatctcaag tccggtcaac acttgaatca gctgatcaga agctccggag ggatatttag
    28981 tcgggatcgc ccgcacaaac atatcgttcg agccttttaa agcggtttct aaagcttgaa
    29041 tagcgtgcgg tttgttgtct tcaatcgcaa tcaccacttc ttccgggcga agcacataac
    29101 gcagaatgcg aataccttcg ataagatccg cagtgaaatc ttgcattaag cgatcgtcac
    29161 aggtgatgta aggctcacat tctgccccgt taatcaccag taatttacag cgtttatccg
    29221 ccaaacttaa ttttgttgcg gttgggaata ccgcaccgcc taaaccggca atgccatatt
    29281 ggtaaacttt ttggataatt tgatcgcttg ttaggcttaa aaaatcctca atcggctggc
    29341 gttccaccca tttatcttta ccatcggttt caataatcac cgttatttcg ggcaaacctg
    29401 aggcgtgagc ggaaacataa ggctcaatcc caatcacccg cccggaggtt gggctgtgta
    29461 cgggcagttg acggaagtga tcgcctttcg tgagcggctg ccctttcaat accacatcgc
    29521 cgactttgac taataattcc cctgcccagc cagaatgttg aaccagtggc acatagaaaa
    29581 acttcggtaa atttaaggtg cgaattggct tgctgtttga ttgggattta ttttccatcg
    29641 ggtgaatacc gcccgggaaa tcccacagtt tacccgtttc tttattttgg cggattcgct
    29701 caatcactaa attaggattg ctcatttttg cctccgccgg taactaattt tctctgcaat
    29761 tcagtggtat tcacaatcgg aataattaaa tcttggtcaa atttccagtt ccaagattgg
    29821 gttgtcggtt taattggtcg catttcaata cagttggtcg ggcaaggggc gacacaaagt
    29881 tcacagccgg tgcaaagatc cggaataatg gtgtgcattg ctttattcgt gccgataatc
    29941 gcatcaaccg ggcaagcctg aatgcactta gtacagccaa tgcagagatc ttcaatcaca
    30001 aaagccactt tcatctcagg ctctgccata ccttccattg gcggcacttc taccccaagc
    30061 aattcggcaa gattcaccac taaaggttgt ccacccggca cacatttagt gatcacatca
    30121 ccattagcaa tcgcctcagc ataaggctta cagcccgggt agccacattg accgcattgg
    30181 ctttgtggta aaagggcatc aatttgatcg acaatcgggt cggattcaac tttcagttta
    30241 atcgaggaat agcctaaaac cgccccgaaa atcagtgcga tcacagcaat agcgataaga
    30301 atataagcga taattggcaa atcaagcatt agagtttcac caatcctgta aagcccataa
    30361 aggcaagcga catcaagcct gcggtaatca gagcaattga tgctccttgg aacggacgag
    30421 gcacatcagc ggcagccaaa cgctcacgca gagccgcaaa taaaactaac accagcgaga
    30481 aaccggcagc ggctccaaaa ccataaagaa cagattccac caagttattg gctaaattca
    30541 cgtttaataa tgccacccct aatacggcac agttggtggt aatcagcggt aaatagatcc
    30601 caagcaagcg ataaagtgtc gggctggttt tatgcacaat catttccgtt aattgcacga
    30661 tcaccgcaat cactaaaata aacaccaagg tacgcaaaaa ttgagcgttt aacggctcaa
    30721 gcaaataggt ttccatcaaa taagcagaga gcgaggcaac cgtcagcaca aaggttgtcg
    30781 ccaaccccat accaatagca gtttccacct ttttcgacac tcccataaac ggacaaagcc
    30841 ctagaaattt aactaagaca aagttattaa taagggcggt gctgatgacc aacagaatat
    30901 aatcgaccat aaattccaca aaattaaata tacaagcggt caaaaacgca ttttattttg
    30961 caaaaaacag agaaaatctg accgtttgtt aaagcatctc attaaaaata aacgttatat
    31021 tatctcttgt tttcggctca ttcacaataa gaaatcggag gtacttctag taaacatacc
    31081 atatcaaata tgatcttgtt acctttgttt ctatctctgg tagaatggag aatgtgggga
    31141 ctaagctata cagcatgaaa ggtgaaagga gcttgatttt ctaagttaat cctaaaaagt
    31201 ctcactcctt tgatatagag gatattagaa
//
LOCUS       DANLXQ010000010        30128 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.010, whole genome shotgun sequence.
ACCESSION   DANLXQ010000010 DANLXQ010000000
VERSION     DANLXQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 30128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30128
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.010"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            182..928
                     /locus_tag="PXD94_000361"
     CDS             182..928
                     /locus_tag="PXD94_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL6285264.1"
                     /translation="MQLRHKQIMEYIQQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            1124..2887
                     /gene="fucI"
                     /locus_tag="PXD94_000362"
     CDS             1124..2887
                     /gene="fucI"
                     /locus_tag="PXD94_000362"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL6285265.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLHFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDSVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGLSSQFLTK
                     GGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRANETWPTTWFVPRLTGK
                     GAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCVHNVEDKDVFRPSAWNG
                     FGQDKEGQDYRACANFGPLYK"
     gene            3081..4514
                     /gene="fucK"
                     /locus_tag="PXD94_000363"
     CDS             3081..4514
                     /gene="fucK"
                     /locus_tag="PXD94_000363"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL6285266.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVESPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYLLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAMKPNVKRVEPSSNSQFYK"
     gene            4589..5020
                     /gene="fucU"
                     /locus_tag="PXD94_000364"
     CDS             4589..5020
                     /gene="fucU"
                     /locus_tag="PXD94_000364"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL6285267.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            5080..5733
                     /locus_tag="PXD94_000365"
     CDS             5080..5733
                     /locus_tag="PXD94_000365"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL6285268.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            5795..7324
                     /locus_tag="PXD94_000366"
     CDS             5795..7324
                     /locus_tag="PXD94_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6285269.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            7321..8319
                     /locus_tag="PXD94_000367"
     CDS             7321..8319
                     /locus_tag="PXD94_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6285270.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            8343..9290
                     /locus_tag="PXD94_000368"
     CDS             8343..9290
                     /locus_tag="PXD94_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6285271.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            9346..9597
                     /locus_tag="PXD94_000369"
     CDS             9346..9597
                     /locus_tag="PXD94_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL6285272.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            9726..10877
                     /gene="fucO"
                     /locus_tag="PXD94_000370"
     CDS             9726..10877
                     /gene="fucO"
                     /locus_tag="PXD94_000370"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL6285273.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAVQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
     gene            11144..12589
                     /gene="aldA"
                     /locus_tag="PXD94_000371"
     CDS             11144..12589
                     /gene="aldA"
                     /locus_tag="PXD94_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL6285274.1"
                     /translation="MVKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
     gene            12845..13492
                     /locus_tag="PXD94_000372"
     CDS             12845..13492
                     /locus_tag="PXD94_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL6285275.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(13625..15001)
                     /gene="trkA"
                     /locus_tag="PXD94_000373"
     CDS             complement(13625..15001)
                     /gene="trkA"
                     /locus_tag="PXD94_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL6285276.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARIRSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     RAVMSELQRLDRPHKRIMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSK
                     TMVLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPA
                     YLHLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTS
                     KVVGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKL
                     FQLGVFFL"
     gene            complement(15001..16332)
                     /gene="rsmB"
                     /locus_tag="PXD94_000374"
     CDS             complement(15001..16332)
                     /gene="rsmB"
                     /locus_tag="PXD94_000374"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL6285277.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            complement(16384..16809)
                     /locus_tag="PXD94_000375"
     CDS             complement(16384..16809)
                     /locus_tag="PXD94_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL6285278.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            complement(16902..19550)
                     /locus_tag="PXD94_000376"
     CDS             complement(16902..19550)
                     /locus_tag="PXD94_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL6285279.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDRIYP
                     SERHIEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNL
                     GLTLIQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWI
                     FIPALLLVFGIVANQRRRVKKQCVIA"
     gene            complement(19752..20714)
                     /locus_tag="PXD94_000377"
     CDS             complement(19752..20714)
                     /locus_tag="PXD94_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL6285280.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
     gene            complement(20898..22745)
                     /gene="selB"
                     /locus_tag="PXD94_000378"
     CDS             complement(20898..22745)
                     /gene="selB"
                     /locus_tag="PXD94_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL6285281.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLALVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDELQYGRKLTVQLIEYFDRSGFLRRKGDVHLLRDSETF"
     gene            complement(22755..23051)
                     /locus_tag="PXD94_000379"
     CDS             complement(22755..23051)
                     /locus_tag="PXD94_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6285282.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(23048..23224)
                     /locus_tag="PXD94_000380"
     CDS             complement(23048..23224)
                     /locus_tag="PXD94_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285283.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(23293..23925)
                     /gene="pdxH"
                     /locus_tag="PXD94_000381"
     CDS             complement(23293..23925)
                     /gene="pdxH"
                     /locus_tag="PXD94_000381"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL6285284.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSEAINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGWEIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(23983..25365)
                     /locus_tag="PXD94_000382"
     CDS             complement(23983..25365)
                     /locus_tag="PXD94_000382"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL6285285.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIELSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVAE
                     FDKLIEMLEY"
     gene            25433..25527
                     /locus_tag="PXD94_000383"
     tRNA            25433..25527
                     /locus_tag="PXD94_000383"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25467..25469,aa:Sec,seq:tca)
     gene            25734..26423
                     /locus_tag="PXD94_000384"
     CDS             25734..26423
                     /locus_tag="PXD94_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL6285286.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EMPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            26433..27335
                     /locus_tag="PXD94_000385"
     CDS             26433..27335
                     /locus_tag="PXD94_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL6285287.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCVLENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            27360..28238
                     /gene="nanA"
                     /locus_tag="PXD94_000386"
     CDS             27360..28238
                     /gene="nanA"
                     /locus_tag="PXD94_000386"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL6285288.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGAIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(28299..28505)
                     /locus_tag="PXD94_000387"
     CDS             complement(28299..28505)
                     /locus_tag="PXD94_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285289.1"
                     /translation="MDNKQTNGFNDIIVKADITQNIWSTGFYDIGRELKSENTRQEKV
                     RLKYNGDTLHRGRKSRMASLIASR"
     gene            complement(28463..29101)
                     /locus_tag="PXD94_000388"
     CDS             complement(28463..29101)
                     /locus_tag="PXD94_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285290.1"
                     /translation="MKKILLLVLLLTLATQADEGNNTQVCAEQQHQIIAHYFKISQEK
                     LSARTLASACKTAPNNSTETLVSYAVIEPKYSKENYQNDENTPKWTLFVARVNDNAIL
                     QSWEQDMEIDASIDVNADSLKIDTARYILNDKVRAFGVRFNNAARGPSAPEYSANDHL
                     MLFVPEKEKLKLVFDYPMTFWSILDDKETKTETQTWYCKWITNRPTASMILL"
     gene            complement(29343..30062)
                     /locus_tag="PXD94_000389"
     CDS             complement(29343..30062)
                     /locus_tag="PXD94_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6285291.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINSFRTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADSDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
BASE COUNT         8790 a         6507 c         6367 g         8464 t
ORIGIN      
        1 tgattgtcaa ttagtctgaa atgaaatttc acaaaatata ttacaaattt tacaaaaagt
       61 tccctaaatt taaccgcttg caacacaaaa tgtgatcgcc ttctaaaaat caaatttgct
      121 tgctcgacag taacgctaca agcggttaaa tttggagaaa aatttacaaa aaggatagat
      181 tatgcagctg cgacacaaac agataatgga atatatacaa cagtttgatg aagctaacgt
      241 tcaagaattg gcggaacatt gtggggtttc gattgaaact atccgccgag atttaaataa
      301 gctagataaa gatggattat tacatcgcac tcacggtgga gctgtgagcc ataaaaagcg
      361 agatattggt cgctcattta ccacccgaca acggctgaac agtgaggcga aacgcagtat
      421 cgcagaaaat gtgttggaat atttacaacc agaatcggtg attgctttag atgccagctc
      481 aacctcttgg aatgtggcac agcgtattcc aaatattcct tgcaccgttg tcagcagttc
      541 aatgcggatt atctacagcc tttcacacaa accgcacatt aaaacgattg ccaccggtgg
      601 tatttattcc gaaaaatacg atgccttcta tggtccatta tccgagcagt tactgacccg
      661 tttaaaaatt gatcttgcca ttctttcttg tacaggcatt gccgatgggg cgatttggga
      721 atcaaatgaa attaatattt cccttaaacg taaattaatt gcgaacagta aacaggtttt
      781 tttattagcc gatcacagta aatttgagcg taaagacttg tttcgtatcg aagatttatc
      841 ttgcattgat aaattattta tcaaccaacc cccaccggac tttctcaaaa attattgctt
      901 agcacatcaa attgaaatca ttatttagtg atttctgccc gattttattc agaaataaaa
      961 caaatcgggc attctttttt cactcatttt taccaaaatc ccccattttt tgtgatcgca
     1021 ctcacaaaat ttaaactaaa ttcgcatttt tcttcataac tcatattgcg tatgcataat
     1081 aaattcaatc gcaaattttt ttaaccaatc gggagaacta tttatgacaa cgttgaaatc
     1141 tacccctgta aaaattggta tccgcccaac cattgacggt cgccgtttag gagtgcgtga
     1201 atcacttgaa gatcaaacaa tgaatatggc aaaagccgtt gcacaattaa ttcaatctga
     1261 agtccgccac ccaaatggtg atttcgttga atgtgtgatt gctgattcca ccatcggtgg
     1321 tgttgccgaa gctgcggctt gtgcagaaaa attcaaacgt gaaaatgtcg gagcggtgat
     1381 taccgtaacc ccttgctggt gctacggttc tgaaaccatt gatatggacc cacatatgcc
     1441 gaaagccatt tggggcttta acggaacaga acgcccaggt gcagtttatt tagctgctgc
     1501 tttagcaggt catagccaat taggtttacc ggcattctcc atttatggca cagaagtaca
     1561 agaagctgac gatcaatcta ttccagacga tgtgcgtgaa aaattactcc actttgcccg
     1621 tgccggctta gcagtggcaa ccttacgagg aaaatcatat ttatctatcg gctctgtttc
     1681 aatgggtatt gccggttcta tcgtaaacca acaattcttc caagaatatt tgggtatgcg
     1741 taacgaatat gtggatatga ccgaaattaa acgccgttta gatcgccata tttacgatgc
     1801 cgaagaattt aaattagcaa tgagctgggt gaaaacctat tgcaaagaag gtattgatgt
     1861 caacgcaccg gaaaatcaac gcagcccgga agagcgtgaa aaactctggg aagacgtggt
     1921 gaaaatgacc attattgccc gtgatttaat ggtgggtaat cctcgcttag cagagctagg
     1981 ctatagtgaa gaagccttag ggcataatgc gattgctgcc ggtttccaag ggcaacgcca
     2041 atggacagac catttgccaa acggcgattt tatggaagct accttgaatt cgacctatga
     2101 ctggaacggc gttcgcgcac catacattat ggcaactgaa aacgacagct taaatggcgt
     2161 gaatatgttg ttcggtaact tattaaccgg tcaggcacaa atctttgccg atgtgcggac
     2221 atactggagc gaagactcgg tagaacgtgt caccggctgg cgtccggaaa gcggttttat
     2281 ccacttaatc aactccggct ctgcggcgtt agacggaaca ggtcagcata ccgatagagc
     2341 aggcaaacca actattaaac cggcttggga agtgactgaa gaagacggta aacgttgttt
     2401 agaaaacacc tgttggtgtc cggcagttca cgaatatttc cgtggtggtt tatcttccca
     2461 attcttaacc aaaggtggaa tgccgtttac gatgcaccgt atcaacctga tcaaaggtat
     2521 cgggccggtt ttacaaattg ccgaaggttg gtctatcgac ttgccggaaa atgtacacga
     2581 cattttaaac aaacgcgcca atgaaacctg gccgaccact tggttcgtac ctcgcttaac
     2641 cggaaaaggc gcatttaccg atgtgtatag cgtaatggcg aactggggtg caaaccactg
     2701 tgtggcaacc ttcggacacg ttggggcaga cttaattacc ctcgcctcaa tgctccgcat
     2761 tccggtttgt gtgcacaatg tggaagataa agacgtattc cgcccaagtg cgtggaacgg
     2821 tttcggtcaa gacaaagaag gtcaagacta ccgtgcttgt gctaacttcg gaccattata
     2881 taaataatac acaatcaccc tctccccttg tgggagaggg acagccaagc aattcgttca
     2941 gaattgaatg gcagggagag ggctacaagc ggtcgctttt ctctcaaaat ttacaaatcc
     3001 ctctctctga ttaaattgct aaacgcaatt taatctatct ctctaaaagg gagatattag
     3061 gataagtaaa ggagataaca atgccaattt ctcttatttt cgactgtggt gccaccaacc
     3121 tccgtacgat tgcgattaat caagcgggtg aaattgtggc agcccatcat ctaccgaata
     3181 acacacaacc agatgtggaa agcccagaat ttcatatttg ggatattgaa gagatctggt
     3241 cgaaattaat gacctgtgcc aaacagacgc tttctcaact ttcagaacaa caacggcaag
     3301 agattgttgg gatttcagta accacctttg gcgtggacgg tgcattattt gacaaaaacg
     3361 gcaagcagct ttatccgatc atttcttgga aatgcccgag aaccctaccg attatggaaa
     3421 accttgccaa ttatcttgat gttgaggcgt tatatcaacg taatggcgtt ggacattata
     3481 gtttcaacac cttattcaaa ttattgtggt taaaagagaa caaaccagaa atttatcaac
     3541 aagctgagag tttcttattc atctcatcta ttttgactta ccttcttaca ggcgtgcaaa
     3601 caaccgaccg cacaatggcg ggaacgtcaa tgatgacaaa catcgaaaac gattcgtggg
     3661 ataaaacagt tttagattta ttaggcttaa ccgaaaacca ttttcctcca atgaaaagtg
     3721 caggagaagt tgtgggaaca ttaaaaggag aaatttccca agagttaggt ttaagcggtc
     3781 aaattccagt aatttcttgc ggacacgata ctcaatttgc catttttggc tcaggagctg
     3841 gctataacca acctgtgtta agttcaggca cttgggaaat tttaatggtt cgtaccccaa
     3901 aagcaaaacc acaatggcaa ttcgttcaaa acggcttaac cattgagttt gatagccaat
     3961 ctggttattt caacccaggt gtgcagtggg tagcttcagg tgtgatggag tggtttggca
     4021 aacgtttctt ctctgatata gccaatactg aacattacta ccgcacaatg attgaagaag
     4081 cggcagaagt gcctgctggc tcaaacggtg tgaaattagt cggcaatttt gatggcacaa
     4141 cagctaaaac aggttcaatt atcggcttat ctatgcacat cactcgtggc gaaatttacc
     4201 gtgcaggctt ggaatatatg gctcatcgtc taaaagaagg gttagatgta ttacaagaag
     4261 tcggtcattt taatgctgag agtttgattt gtgtgggagg tggttctaaa aacgctttat
     4321 ggaatcaaat tcgagccgat gtcttaaata gaccgattga tgtagtggat ttccctgaaa
     4381 gtaccgtatt aggcgcggca atgtttactt ttacaggtgc tggtgtattt gaaaatccaa
     4441 atgcggctca acaggcaatg aagccaaatg tgaaacgggt tgagccatcg tcaaatagtc
     4501 aattttataa ataaagtttc tcccctctct ctgatttgag gttcattcag aacatcaaat
     4561 cagagagagg gtaaaaaagg agttcaaaat gttaaaaggt attcacccgg caatttctcc
     4621 agatttatta aaagtcttag ccgaaatggg gcacggcgat gaattagtgc tttctgatgc
     4681 acacttccct gctcactcta ttcatcaaaa cgtcattcgt gcagatggct taagtgttgc
     4741 caccttatta gaaggcattt ctgctctgtt tgaatttgac caatacgttg aagccccact
     4801 tgcaatgatg caagcagtaa aaggcgacac gctcgatcca agcgttgaag agcgttacct
     4861 caatgcaatt aaaaaagtaa acggaacagc cccgaaaata gagcgtgtag aacgcttcgc
     4921 tttctatgat agagccaaaa acgcttatgc cgttgtcatt acaggcgagt tagccaaata
     4981 cggcaacatt attatcaaaa aaggcgtaac accggtttaa ttacaagcgg ttaaaaaagc
     5041 agaatttttt acaaatataa aattaaaagg agttcaacaa tggacagaaa aatactggcg
     5101 agacaaatca ttgatacttg tcttgaaatg acccgaattg ggttaaatca aggtacagca
     5161 ggcaatgtga gcgtacgtta taaagacggt atgctcatta cgccaacagg tacaccttat
     5221 gagcaaatga ccgaagattc tatcgtcttt gttgatggca acggcaaaca cgaagaggga
     5281 aaactccctt caagtgagtg gcagttccat ttagcggttt atgaggctcg ccctgaatta
     5341 aatgcggtag ttcacaacca cgctaaaaac tgtgccgcag tgtctatttt aggtgaacct
     5401 atccctgcca ttcactatat gatcgcctgc accggcacta atcacattcc ttgtgtacct
     5461 tatgccccgt tcggcactca ccaattagcc gactatgtac gtgaaggcat taaacacagc
     5521 aaagcaattt tactcgctca ccacggctta atcaccgcag ataaaaccct cgaaaaagcc
     5581 ctcggcatcg cccacgaggt ggaagtgatc gcagaatggt atttgaaact gctcgctaca
     5641 ggaaaaccta tcccaacgct aaacaaagaa caaatggata tcgtactcgg aaaatttaag
     5701 tcctatggtt cttgggtgga aagcgaacaa tagcctgcca taaataacat acaagcggtg
     5761 atatttctta aaatttttac aaacggagtg cagtatgcaa aatgaagtaa tactcacgat
     5821 gaaaaacatc agtaaatcct ttgcgggggt caatgcgtta aaaggtgttg agcttacgct
     5881 gcgtaagggc gaagttcacg cattaatggg cgaaaacggt gcaggtaaat ccaccttgat
     5941 gaaatgcttg ctgggcgtat atcaagccga tgaaggtgaa atcatatata aaggtcagcc
     6001 tgttcgcttt gagtcggtat tagaggcaca aaaagcgggc attagtatga ttttccaaga
     6061 actgaacctc attccccatt taacggtggc agagaatatt ttcttcgccc gtgagccact
     6121 caaacacggt ttagtggata aagacaaaat ggtggaagaa tcggctaaat tgctgaattt
     6181 ctttgagatt gatgttgatc caaacgatga agtgagaatg ttgtcggtag ccaaacaaca
     6241 gatggtagaa attgccaaag ccctttcatt tgatgtggaa gtactgatta tggacgagcc
     6301 gacctccgct cttaccgaaa aagagatcga taaattattt gaattggtcg atagactgcg
     6361 tgccaaaggg gtgagcattg tctatatttc tcaccgaatg gaagaattaa aacgtatctg
     6421 taaccatatt acgattttcc gtgatggcac ctatgtatct aaccataaat tcaatgaaat
     6481 cacgatggat gagatcatta ccaaaatggt tgggcgttca ttggataacc atttcccacc
     6541 aaaaaccgct gtggtgacaa acgacattat cctctcgatt atgaatgctg aacgtaaggg
     6601 agtgtttgaa ccattgaact ttgacttacg caaaggagaa attctgggca ttaccggctt
     6661 ggttggagca aaacgtactg aactggctcg agccatcttc ggagcagacc cacttgatag
     6721 cggtgagatt tttgtgcacg agaacaaagt cagcattaaa gatccgagtg atgcaattaa
     6781 agccggtata gcctatttgt cggaagaccg taagctcaat ggtgttgcag taagaatgtc
     6841 gattcgtgag aatatcacga tggcatctat ggataaagtt gccaatcaaa taggtgtgat
     6901 ttcttacgat gaagaagaaa aagcctcaaa aactttcatc gacaaaatgg aaatcaaaac
     6961 cccaacgatt gagcagaaag ttcaaaactt aagtggtggt aaccaacaaa aagtggtaat
     7021 tggtaaatgg ttattccgtg aagcaaaagt gatgattttt gatgagccga ctcggggaat
     7081 tgatgtcgga gcaaaatatg ctatctatca actgctcgat gagcttgctg ccaatggcgt
     7141 gggtgttatc gtgatctctt ccgaattgcc tgaagtcttg ggagtttccg accgcatcat
     7201 tgtaatgcgt gaaggtagaa tgacaggtat gctcgagact aaaaaaacca accaagaaga
     7261 aattatgcat tatgcaactg gtgttaaaaa tatgtttgct cgtgaatatg gggtggaaaa
     7321 atgaatgaaa aacaaaaaga aatgttaaga aaaatggctg cattggctgg cttagtacta
     7381 ttaatcatct ttttcagcgt aaccaatgaa tacttcttta cctccaataa cattatgaca
     7441 gtagggctac aaacctctac cattgcgtta attggtattg gggcaacctg tgtgatctta
     7501 accggtggaa ttgatttaag taccggttca gtagttgcac tctccggtgt ggcagctgca
     7561 atgattgtaa atgccggtgt acccgtacca ctaggaatga ttttagggat tttggtcggc
     7621 ggtatttgtg gcttaatcaa cggcatttta gtcaccagaa tgaaattgcc tccgtttatt
     7681 gctaccctcg gtatgatgat ggttgcccgt ggtttagcat tgtatgttac caatgccgca
     7741 ccagtttccg gaatgcctga atcctttgcc aatctcggta acggagcatt atttaaaatt
     7801 gtggaagaag gggcaaacgg cttgccgaaa gtggtatttg caggtattcc ttaccctgtg
     7861 attattatga tctttattac cgtactattc actttcgctc tctctaaact gaaagtcgga
     7921 cgctatcttt acgcaattgg ttctaacgaa gaagccgcac gcttatccgg cattaaaacc
     7981 aatattgtca aaatctatgc ctatgttgcc agtggcttac tctctggttt aactggcgtt
     8041 atccttgcct cccgcctcgt caccgcccag ccaaacggtg gggtctctta cgagctggat
     8101 gcgattgcca gtgctgtggt gggcggcaca tcgcttatgg gcggtgtggg tactatcccc
     8161 ggcacactca tcgggtcatt cattatcggg gtactccgta atggtttgaa tatgaatggc
     8221 gtttcttcat ttgttcaaat gattgtcatc gggcttgtga ttatcgtggc ggtctctctc
     8281 gaccagctcc gccaatccaa aaaagccggt aaaaaataat ccactcttaa aggagaatta
     8341 aaatgaaaaa atcagcatta tttactgcga ccctctttgg tttatcgacc ttatttgcag
     8401 gcagtgcaat ggcaaaatct gatgaaatcg ctgtgattgt gaaatctgcc aactctacct
     8461 tctggcaaaa cgtgcgtaaa ggggctgaaa ctgccggcaa agatattgga ggacaataca
     8521 aagtcacctt ccaagggccg gaagccgaaa cagcgattga cgcccaagtg aatatggtgg
     8581 acaacgccgt aaaccgtggc gtggcaggca tcgtactggc tgcctcagac ccggtagcat
     8641 tagtgccggc ggtgaaaaaa gcttatgaga gtggcattcc ggtggtgctt atcgattccg
     8701 gcatcaacag tgacggcaaa tactaccaat ctttccttgc caccgacaac cgtgcagcag
     8761 gtaaattagc agcggaaaaa ctgcttgcca aagtcaaagg cggcaaagtg gcagtgatga
     8821 gctttaccca aggggcagca tcagcgattg agcgtaccgg tggttttatt gacgaaatca
     8881 aagcaaaagc cgattacaaa ctggtcggcc cgtactactc cgattcggat atgaccaaag
     8941 cgttaaacca aaccgaagac gtattaggtt ctaaccctga tcttgcagcg atcttcgggg
     9001 cgaacgaacc gactgcggta ggaatggcac gagcggtgaa acaaaaaggc tttgccggca
     9061 aaattgtagc ggtgggcttt gatggaaact ccgacctaca aaacttcgtg cgtgacggaa
     9121 cactagacgg catcgtggtg caatcttcct accaaatggg ctataaaggc gtggacacca
     9181 tcggcaagct gattaaaggc gagaaagtcg aaaaagtgat tgataccggg gtggtttatg
     9241 tcacgaaaga aaacattgat agcgaagaag cgaaaggtgt tctttactaa cattcaagtt
     9301 aaacggggtg gggttgccca ccctttcagc cgaaaggaag ccaatatgtc aaataccctt
     9361 accgataaag ccttagtggt gattgccgca cttggcggtc gagaaaatat tgtcgatgtt
     9421 tacgcctgta ttacccgcct acgtgtggag gtgaaaaata ccgacattgt ggaatgggat
     9481 aaaattaaat ctgtcggtgc gttaggcgtt gtgacctcag gctcagttat tcaatctatt
     9541 tttggtagcg atgccgaagc ctaccgtgat gaaatcaaac gtattttgaa tatttagcct
     9601 aagcacactc acaacaaaag aatcgcaaca agcggtgaaa tttttatcaa aatttgcaaa
     9661 aaaatgacgt aatttgaccg cttgcaagtt aatcgaattg tttaactttt aagaaggaaa
     9721 atagtatggc aaatcgtctt atcctcaatg aaaccagtta ccacggcaaa ggagcaatcc
     9781 aaaatattgt tcacgaagtg accagccgga gtttcaaaaa agcccttgtg gtaacagaca
     9841 aagacctgat caaatttaac gttgcccaaa aagtcaccgc cttgcttgat gccgcaggct
     9901 tgccttatga gattttcgat gaggtcaaag ccaacccggc agtcgatgtg atcaaagcgg
     9961 gggtcgcaaa attcaaagcc tccagtgccg attatttaat tgcgattggt ggcggctcac
    10021 cgattgatag tgccaaagcg attggtatta tcatcaataa ccctgagttt agcgatgtac
    10081 tttcattgga aggtgtcgcc ccaacccacc aaaaatgtgt gccgattatc gccattccaa
    10141 ctaccgcagg tacagccgca gaagtcacga ttaactatgt cattaccgat gaagcgaaaa
    10201 aacgcaaatt tgtgtgcgtg gacgttcacg atgtgccggc agtggcgtta gtcgatccag
    10261 atatgatgga atctatgccg aaaagcttgg ctgccgcaac cggtatggac gctctcaccc
    10321 acgccattga aggctacatt accaaagcgg cgtgggaatt aaccgatgct ctacacctta
    10381 aagcgattga gctgatcggc cgttcattgc gtggtgcagt ggagaaaaat ccgaaagatg
    10441 tcgaggcaat ggcattggga caatatgtgg ctggaatggg cttctccaac gttgggcttg
    10501 gcgcggttca cagtatggca cacccgcttt ctgcctatta cgacacccca cacggcattg
    10561 ccaacgcagt attactgccg tatgtgatgg aatttaataa aaattacacc ggcgaaaaat
    10621 accgtgaaat tgcccgagca atgggggtaa aaggcgtaga tgaaatgagc caagaagagt
    10681 accgcaacgc cgccattgct gccgtacaac agctttcaca agatgtcggc attccgccaa
    10741 aactgcatca aatcggcgtt aaagaagccg atttagcggc attatcggtc gatgccttta
    10801 atgatgtctg caccggtggc aacccgagag attgtacggc tgaggagctg ttggaggtat
    10861 atcggatcgc atactaagtt ctatctttgt taaatagaca agcggtgatt ttttaataaa
    10921 aatttgcaat ttctgttaaa aaaatactga tagtcataat gcccgatttc tcatatttat
    10981 ggaaaaatcg ggcattttcg ttgctttttt atcgccaaga aatgtgattt tgttcacatt
    11041 tatatctata aattaacaaa aataaaacaa tttgtttgca aaggcggttc agttaccgaa
    11101 tgatagtcat caacttccac tgtggataaa aaaggagcaa ctcatggtga aacaacataa
    11161 aatgtatatt aatggtgaat ttgtgcctgc gttagatggt cgcactttag cagtctataa
    11221 ccctgccacc gaaaaagcta tctccgaagt ccctcgtggc ggagctgatg atgccaaaat
    11281 cgctatcgat gctgctgaag ccgcccaaga agcttgggaa cgtttaccag cgattgaacg
    11341 aggaaaatat ttacgtcaaa ttgctgtggg gattcgtgag cgtgccgatg aaattgctcg
    11401 cactatttcc gaagaaatgg gtaaaactct gtccttagca accgttgagg tacattttac
    11461 cgctgactac ttagactata tggcagaatg ggcgagacgt tatgaagggg aaattatcca
    11521 aagtgatcgc ccaaatgaac atattttcct atacaaaaaa gcgattggcg tcaccaccgg
    11581 cattttacct tggaacttcc cgttcttcct cattgcccgt aaagcggctc cggcattagt
    11641 aacaggtaat actattgtga ttaaaccgag tgaagatgca cctaataatg cgattctctt
    11701 ttcggaaatc gtgcatcaag tcggtttacc taagggggta tttaatgtcg tcaccggcta
    11761 tggcaatgaa gtcgggccgg aactttcagg caatgaaaaa atcggtatgg tttcctttac
    11821 cggtagccag tttgccggac aaaaagtgat ggaagccgcc agcaagaata tcattaaagt
    11881 caatttggaa ttgggcggaa aagcaccggc tatcgtatta gacgatgcag atcttgagct
    11941 tgctgctacc gctgtagtaa attctcgaat catcaattca ggacaggttt gtaactgtgc
    12001 agaacgcatt tatgtacaga gttcaattaa agagcaattt gtccaaaaat tggtagagaa
    12061 aatgcaagcg gtgaaatatg gtaatccatt agaacaacaa gacattgata tggggccgat
    12121 ggtaaatgaa aaaggcttgc ataaagtgca ggaaatggtg acacacgcac tgcatcaagg
    12181 aggtaatctg gtattaggtg gtaacgtggt tgaaggaaca ggctactact ttgagccaac
    12241 tattatcgac aatgtagata actctatgga tattatgcgt cacgaaattt ttggtccggt
    12301 cattccggtt gcgacatttg ataccattga tgaagcgatt cgcttagcca atgattgtga
    12361 atatggctta acctcttcgg tttacacgca aaatatcaat aaagcgatga aagtaatttc
    12421 ccgcttaaaa ttcggagaaa cttatgtaaa ccgtgaaaac tttgaagcaa tgcaaggcta
    12481 ccacgccggt tggcgtaaat cgggtattgg cggtgctgac ggtaagcacg gcttagagga
    12541 atacctacaa actcaggtgg tttatctgca atttgatgat aaagtataga ttcttctaaa
    12601 aactcccact tatcaaaata agcgggagtt ttcttttagg ttaaccttgt atcaaaattg
    12661 caaacaaaaa tattctaatg ttaagtaaat gtttaataat tatttatggt taactctttt
    12721 tggctaatta atttgcaatt taaattaatt gagtgcagaa tgccattagc cttttttgaa
    12781 atcacattcc ataaaatggc ttgattaaca caacacaatt atcactcaaa ttaggatttt
    12841 cattatgagt attttccaac aaaccaatcc aaaccgccgt cgttatgggc tagctgtatt
    12901 tatcgggatt attgccggta ttatttcagc attcgtaaaa tggggggcag aacatccttt
    12961 cccacctcgt agccctatcg atctgtttac cgctgcttgc ccgcaacctg tgttagatgc
    13021 aatgaatgcc gcagcagacc aagtggctgc caaaaccatc gcattacaag aatgttcacg
    13081 tgcattttta aacccaccac acgttttctt acgtgattat attggtatcg atccaactga
    13141 agcagcattt acctttgctg accacgcgtt caactggatt ggtgtcaccc atatgatttt
    13201 ctcgttagtc tttgcgattg gttactgcat tgtggcggaa atcttcccaa aaattaaatt
    13261 ctggcaaggt atcggtgccg gtatcatcgc taatatttgt gttcactaca tcaccttccc
    13321 ggcattaggt ttaacaccac cggttgctga atggccgtta tatgaacaca tttctgaatt
    13381 agttggtcat attttctggt tctggaccat tgaggtgatt cgtcgcgatt tacgtaaccg
    13441 tattacgcgt gaacctgatg ccgaagtgcc gttagatcaa ccgttccgtt aatttaaaat
    13501 tagctcgatt tatacaaaag ccgcaatcac gttgcggctt tcttatttta caagcggtca
    13561 aattttcatg aaaatttacc aaacaaaaag cacattgttt catgtagcaa tgcggagctt
    13621 ggctttataa gaaaaataca ccaagttgga acagtttttc aatttcatcg atctgtttct
    13681 tatcgttcac aaaaataatc acacggtcat tttctgcaat catggtggat ttgtgggcga
    13741 taataacttc ttcgcctcgc accaccgccc caacaattgc cccttgcggt agttttaatt
    13801 cacggatttg tcgtccaacc acttttgaag tggttgcatc gccgtgagcg ataatttcta
    13861 ccgctcctgc aaccccaagt ttgagtgaag aaacttgtaa aatatcgcct tttctaacgt
    13921 aactagcgag agcagaaata gtagcttgtt gtggggaaat ggcaatatca atagtgccac
    13981 cttgaatcag gtgtaaatag gcagggcgtt gcactaaaat aatcactttt ttcgccccaa
    14041 gccgttttgc cagcagggcg gacataatat tcgcttcatc atcactggtg acggcaagga
    14101 ataaatcaat attctcaata tgctcctcaa acagcaactc ttcatcggag gcatcaccgg
    14161 taagcaccat cgttttagat agtttttctg ctaatttttc ggctcgaacc ggattgcgtt
    14221 cgataatttt tacccgatac tgatcttcta aatcccgagc taacgaagag ccgatgctcc
    14281 ctccaccgac aatcataatc cgcttatgtg ggcgatcaag ccgttgtagt tcactcatta
    14341 ctgctcgaat atgttgggtt gcacagatga aaaagacttc atcacctgcc tcaataatag
    14401 ttgaaccttg tggcacaatc gctttgtctt ggcggaaaat tgccacgacc cgagcatcaa
    14461 tatgcggtaa atgatcacgc agggctgaaa tcgggtaacc gaccaaaggc ccaccgtaat
    14521 aggcttttac attcacgata ctgactaact catcagcaaa atgagtaatt tgcaacgccc
    14581 ccggatagtg aataaggcgt aaaatatctt ctttgactaa aatttcaggg gcaataatat
    14641 ggtcaatagg caatacatca tcattaaaca accgctctcg ttctctcaca taatcagcac
    14701 tgcgaatacg ggcaattttg gtcggcacat taaataaagt gtaagcaatt tggcaagcga
    14761 tcatattcac ttcatcacta ttagttacag caactaatag atcagcatca cctgcaccgg
    14821 catcacgcaa ggtttgaggt gaagcatatt cgccccgaat cacctgtaaa tcgtgtttat
    14881 ctttgagctt gtctaaacga gttgggtcat tatccaccaa gacaatatca ttgtcttcac
    14941 tcaccaaatt ttctgccagc gtagaaccaa cttgtcctgc ccctaaaata attactttca
    15001 ttactctgct actttttgta gttttgcgta gaagaaacca tcaccaccgt ttggttgtgg
    15061 gaagaactgt ttttctaaca ctttttcgcc gttaaaatcg atttcaagtt gttttgcatt
    15121 cggtgtatct tgtaaaaacc gttgaatttg ctcgctattt tcttgcggta acaccgagca
    15181 agtggcatat aacaaaatgc cgtttggctt taacctttgc cataacgcct tgagaatctc
    15241 accctgtaat gtggctaatt ctgcaatatc accctcttta cgcaaccatt taatatcagg
    15301 gtggcggcga atgactccgg ttgcggaaca aggggcatca agtaaaatgc ggtcaaacag
    15361 cacaccctct tcaagccatt cctcaggttt ggaagcatcg ccacaaatta cctgtgcaat
    15421 ctgccccagt cggactaagt tttcacgcac tcggcttaaa cggctctctt caatatccaa
    15481 cgccataacc tttgctttcg gtgctttttc taaaatatgg gtagttttgc cacccggtgc
    15541 ggcacaggca tctaaaacgg tttcaccatt ttctgccccg agcaactccg ctgaccattg
    15601 tgcgtgagca tcttgcacgg ttgcccaacc ttgctcgaaa tgtggcagtt ggttcacatt
    15661 taccgctctc tccagcaata aagcacactt tggcacgcaa gcggtcggat tttctgaatt
    15721 ttttgcaaat tcaggctcaa ccagctcgct aatcaacgcc gcataatcat tggtcttgat
    15781 atgttgttga ttgactcgaa tccacattgg tgggcgttgg ttgtttgctt caacaatttc
    15841 tcgccagttc ggataggctt ttttaagttt attgaccaac cattcagggt ggagcgtttg
    15901 ccaatgttta tctaccttcg ctaagatttc ttcttgctct cgcagaaaac ggcgtagcac
    15961 accgttagtc aaggctcgga agctatccag ttttagcgtt ttagttgcat tcactacctc
    16021 atcaaccgca gcgtgtgccg gcactcgcat ataaagcagt tggtataaac ccactaacag
    16081 taagcaatgc accaaacggg ttttgccttt cagcggtttt tcaactaata atttgatgat
    16141 cgattccaag cgaggcagca cacggcacac tccgaaggtg atttcttgca ctaagggtaa
    16201 atcttttggt tcaagctgtt tttgagcgtc aggaatcaag gtggaaagtg atttaccttg
    16261 atctaacacc tgtaaaataa tggttgcact tattgctcta gaagaaagtg ctttgaacga
    16321 ttgttttttc ataaataaaa atgccattcc gacagaatgg cactattgta actgaatgcg
    16381 ggattatctt aaaactaaaa ttggcttggt ggtgttgcga gccactttaa ccgtattaga
    16441 gcttaagccc ttcgcatttg gtttgctgct tgccagcatc ataattaaat ccaccgactt
    16501 ttcatcggca atacggcaaa tttcttcata aatcgtacca tacgccacaa tgtgctgcac
    16561 ttttgaacct tcaggaaaat ggtttttggt aaattcgtgc aatttttggt ttgcttcagc
    16621 caccacttct ttatcgaaat ttttcggcaa gaatgcagaa attaagctgt tatccaccgg
    16681 ctcaataata gacacaaccc gataaaccgc attcggattt tttgccgtca tttgtaatgc
    16741 cgtatctacc acatatttag cactttttag gtcggctaaa tcaatcgcaa taagtaattt
    16801 gttatacata tttattccgt aggggcaggt cctgtgcctg cccgaatatt aactaaaata
    16861 gcggacgggc acaggacccg tccctacata gcatataaat actatgcaat aacacactgt
    16921 tttttcactc tacggcgttg gtttgccaca ataccaaata ccaacaatag agcaggaatg
    16981 aagatccaat ctttagaagg agcatcttgc ggtacggcaa tgtagtgaat ggtttgatcc
    17041 cagtttaagc ctgctttagc tgccggagaa tccatttcta ccatatcaat caccactttt
    17101 ggtgtttcta cgccatcaac tttgattttt tcatcggttt gaattagcgt taagcctaag
    17161 tttttcaaac gctcttcgcc ggttgctcct tccggtacag gtaattgagc gtagaactca
    17221 atttctttac cgtaagggtt taatccctct acttttaaag taacattttg acctactttg
    17281 cttgcaagca actgttcttc aaactgcacc ggctcgatat gacgttcaga cggataaatt
    17341 ctgtccataa agaagcccgg acggaacaag gcgaaagcgg ctacaaccag caacgccgtt
    17401 tcccaccatt tgtttttagt cacaatatat tgcattgtag cagaggtaaa ggcgagaacc
    17461 ccaacagttg cggtaataaa cacaacccag ccgtgccccg gataacgcag atcaattaat
    17521 aataaatcgg tattaaaaat aaataagaac ggtaaaatga cggttcttaa actatattta
    17581 aatgccacca taccggtatt aatcgggcta ccgcctgaaa ttgctgccgc agcgaacgag
    17641 gctaaaccaa caggtggagt cacatctgcc ataatcccaa aatagaatac gaataaatgc
    17701 accgcaatca acggcacgat taacccgttt tgacgaccga cttccatgat aaccgttgcc
    17761 attaatgatg acaccacaat gtagtttgca gttgtcggta agcccattcc caaaattaag
    17821 ctgaatacgg ctaccaagat caacattagc acaatgttgc ccattgatag gagttcaatc
    17881 acacttgaaa gctgaacccc aaagcccgtt agtgccacaa cgccgacaat aatccctgct
    17941 gttgcggtag cgagggcgat accggtcata ttacgaccgc cagcctcaag gctatctacc
    18001 acatctcgaa caccttgttt gataatctct ttggttacgc tctctttacg gaagaaattc
    18061 agtaacgggc gttgggttaa ttgaataaaa attaaggtaa tggttcccca gaatgcggat
    18121 aagcccggcg aaagcatttc caccattaag caccaaagta gcacaaccac aggaataagg
    18181 taatgtaaac ctgcatttac ggttggtttt ctttttggta gagaaacaat ttctgagttt
    18241 ggatcgtccg cttctaaatc agggtagctt gccacacggc gaatagccag tagataccat
    18301 aagcctaaga aagcacagac aatcataaac gcataatctt ctgccacatc acgaatccaa
    18361 cctaagccgt agtaaacgcc aatcgctaat cctacgatca ctaaaattga agctacggtt
    18421 cttagcaaga agactaaaat cgggcttggc tcttcggctc ttggtaagcc ttgtaaattc
    18481 atcttacaag cttctaaatg cacgatatac accaacgcaa tatacgaaat taacgcaggt
    18541 aagaaggcgt gagtaatcag ctggctatac ggcatattca catattcgat catcaagaac
    18601 gctgcagccc ccattaccgg tggcataatt tgaccgttta ctgatgaggc aacttccacc
    18661 gcaccggctt tttctttggt aaagccgacc cgtttcatca ttggaatagt gaatgtacct
    18721 gtcgtcacga cgtttgcaat cgaagagcct gaaatcaagc cggttaaagc ggaagaaacc
    18781 accgccgctt ttgccggacc accacgcaaa tgccctaaat aggcaaaggc ggttttaata
    18841 aagtagttac ctgctcctgc tttatcgagc aacgccccaa ataatacgaa caggaacaca
    18901 tatttggtcg aaaccccaag ggctacacca aacacgcctt cagtggtaat ccattgttgg
    18961 ttgataattt gagaaagcga acctgagcgg tggcttacaa tccaatctgc cgggaaatat
    19021 tgcccgaaga agttatagat caggaataca atcgcaataa tcacaagcgg taagccaaga
    19081 ctacggcggg tcgcttccag caccaataaa atccctaaac agccggcgat aatatcttgc
    19141 gtatttggcg ctccaaaacg tttcaccaaa ccttcataga agaaaatata atacaacgct
    19201 aacccagctc ctacgatggc aaacacccaa tctattttag gaatatgatg tttaggcgag
    19261 ctggcaaaag cagggtaaga taggaacgcc aagaacattg caaaggctaa gtggatagaa
    19321 cgggctttgg tgtcgtccac caccacattc aaattaatgc ctgcgtgagt aagccattct
    19381 tggaatacaa acggcaatgg agaagcataa taaatttgga acacagacca aagaatggcg
    19441 acatacacaa tggctttttt cgcaagaccg gacggggttc gccccccact gtcattggag
    19501 gcaaccatat cttgtaggtc atcgtaatca accttagatt caactgacat aaagactcct
    19561 caaaacgcaa gcggtcattt tcttattaaa ttttacaaat ttcctcaaaa aacaaccgct
    19621 tgctcgtgaa aacactgttg ataagttatc aattaaaggt gaaaatcgat aacttatcaa
    19681 cagtttcaaa aaagcaaaaa accagcacgc taaaaaacgt gctgattgaa tcaaattaca
    19741 gaaaggaaat attacttaat ccaacctttc tctttatagt aacgaattgc accttcgtgt
    19801 aacggagcag aaagggcgtt tttgatcatc tcttcttgtt ttaagtgttc aaacgccgga
    19861 tgtaaacgtt tgaaacggtc gaagttatca aataccgctt tggttacatt gtacacttta
    19921 tctgcatcca catcggcaga agtcactaac gttgcatata caccgaaggt ttctgtcggc
    19981 tcatccgaac ctttatataa accgcccgga atcgtagctt tggcataata tgagtgatct
    20041 gccactaatt tatcaatttc agggccggtt accggcacta atttcgcatc gcaagaagcg
    20101 gctgcttctt ttaacgcacc attcgggtga cctacgttat aagtgatggc atctaagttg
    20161 ttatcacaca ttacagatgc catttctgcc ggtttcaatt cagaagccac tttgaaatct
    20221 ttatccgtcc agccttttgc cgctaaaatc acgttcattg ttgcacgagt accggaaccc
    20281 gggtcgccca cgtttacacg ttttgctttt aaatcatcga acgatttaat gcctgaatcg
    20341 gtacgagcca ttactgtaaa cggttccggg tgaagcgaga aaatggcacg aattttatcg
    20401 tttttcttac cttcaaatga gcttgagccg ttataggcgt ggtattgcca gtctgattgt
    20461 gcaatcccca tatccatttc ttttgctgca atcgcattta agtttgcaac cgatgcccct
    20521 gttgaaggag cgttacattt taaacctgtt tttgctgaat cacggtttac taattggcaa
    20581 atagattgac ccacaacata ataaacaccg gtttgaccgc ctgttccgat agtcacaaat
    20641 gtttcagccg cttgaacaga tgctgttccc atcacaaggc ttgcaaggaa agaaagtttg
    20701 actaattttt tcatttttaa ctcctaaaat taaggatcag aaaacaaaga tttattgttt
    20761 tgagccactg ctcacactac aagagataca aaacttcttt gagttgcatt tgcctcttag
    20821 agcttgtttt tagcatagat tttataatta gtaaatttat tttttcgcat aaaaaaatct
    20881 atgcctaatg ccacaagcta aaatgtttca ctatctctta acaaatgcac atcgcctttt
    20941 cgtcttaaaa aaccgctacg atcaaaatat tcaattaatt gtaccgtcaa ttttcgccca
    21001 tactgcaact catctcgcaa ctggttcacc gaaatgctgc cgttttgggc gataaaatct
    21061 ttaattaacg ttgcaaattg cccgatttgt tcgcttaata aaaatctatc tttgacgatt
    21121 gggattaaat agcccaattt cccgacttta tacagcaaat tccgcatttg ggtttcatca
    21181 atcgagagct ggcttgcaat atccctcacc caaagagctt gattggttgc ctcaaacagc
    21241 ggtcgaattt gttgccaaat ttgcaattca tcggcgttca attcgattcg atgttccggc
    21301 aaatgtaacc aaccacgact attgaccaag gctttttgct ccaccaaatc ctcaataaat
    21361 tgatttgcca agttttcagg caaatccaat aacgctatgc gatataaacg agctttggtc
    21421 acgccgattt gatcttgatg ttgcaggtgg tattctgcca ttttttccaa cacccgttgc
    21481 tgggcttgag ctttaaaatc cgaattaaat aaccatttag acgagacatc aaaccccagt
    21541 tttgcaatat cctctgcaaa acattgctcc gcccataaca gcaaggataa atccatcgct
    21601 ctgttttgca gatagagatc aaggcgtgtc gcaaagtcat tacaagcggt ggtttttgct
    21661 aaatttttta ccaaagctaa acgggcttcg gtacgtttat gccgttttgg tgagtgaatt
    21721 tccagcacct ccgctccggc taacgttagg ctgtcatcac cgctgcggat gatcaactta
    21781 tcaccaacgg caatatgaag tggttcatct aaaatcactt ctgcaaaaaa ttgctgattt
    21841 ttgaccgctt gttgcccctc aagcaaattc aatttgccgg taatgtgcga agcaaaatgg
    21901 taaagatgca caatgctgtt ttctttgaag gcttgattgg cagtgagttg aaccgtaatg
    21961 cgatcggtgg caaatttcgg ctcaagctcg gtaatccaat cgcctcgctt gatttcctct
    22021 ttttccacgt tagcgatatt taacgccaaa cgttgccctg ccactcccgt ttccgaaggc
    22081 gtattttggg cgtgaatcgc tttgacccgc acttttttac cattcgacaa aaataactca
    22141 gagccaacgc tcacttttcc cgccaccgtt gtgccggtaa cgaccaaccc tgctcctttc
    22201 acattaaaca cacggtcaat cgcataacgg aacggtttat gggtttgggt atgttgttga
    22261 tttaattgaa ttaaatgtgc ttttaattct tcaatccctt gttgcgtttt tgccgaagta
    22321 acaaaggttt ttgcttccgc taaaaacgga tagcttgttt tcacttgctc aattaagtgg
    22381 ctgatttgag tttcttccgc ccgatctgcc ttagtaatca caatcataat atgctggaaa
    22441 ttcagcaggt ggagaatatc caaatgctct tcggtttgtg gcttaatccc ttcttcggca
    22501 gaaatcacca acagggcgtg ttgaatgccg ccaagccctg ccagcatatt agataaaaaa
    22561 cgctgatgac ccggcacatc aataaaccct aaaatatcgt tctcaatcgg taaataggca
    22621 tagcccaaat caatggttag cccacgtttt tgttcttcgg gcaaatgggc ggtgttagtg
    22681 ccggttaagg cttgtaaaag cgatgttttg ccgtggtcaa cgtgaccggc ggtgcagatg
    22741 atcattgata gtccttataa aaatgagtaa ctattctggc gagcagattt aaaatctaca
    22801 atatagacat tttcctgacc aatcaggaac tttacttggt acttaccgtt taaaatggga
    22861 atttcttttt tcttgcgaag tcgataaaca tcggcagtaa aggaaagttt atccacttca
    22921 ctttttattg catcataaaa ttgttctgca ataatcgggt tttgggattg ttgtaatata
    22981 taatctgctt gataaaacaa ttttatttct gctttctgta gccaaacaat attgtaatca
    23041 aacttcatca tttttccgtg attcccaatt ttttgcgtaa atcttccatc acctgagcgt
    23101 gggatttagt ttcaattaag ccattttcta attgatgtaa taaatcaagt tcatcattag
    23161 tataaaaact ttgtggctta tctgtgcgta attttgctaa atcttgtgcg agtatgagtt
    23221 ccatatttcc cccttacaaa cttagattat tcaatattgt aacctgataa aactaaagtt
    23281 caaggagtgt atttaaggcg ataaccgctc ttttttccac tcattatttt ccaaccaata
    23341 atgaatgcgg tcgtgtagcc ggctacttct gccttgccag agttcaatac tgtcgggaat
    23401 cagaatatag ccaccccaat gcggtgggcg aggcactgaa agtggatatt tcaatgcaaa
    23461 ttgagctgat tttagcaaca aagccttgta gttttcgata ggctgactct gctcgctcgc
    23521 ccaagccccc aaacggctgg tgtaaggacg agagtcaaaa taggcatcgg attctgcctc
    23581 tggaattttt gctattttcc cttcaatccg cacttgacgt tgtaattctg cccaaaagaa
    23641 ggttaaacat ccaaaaggat tatcggcaat ttcccaccct ttacggcttt gataattggt
    23701 aaagaacaca aagccttgtg aatttacctc tttcaacagc accattcggc tacttggtcg
    23761 cccgtttgct gcaacggtag caatattcat tgctgtcggc tcattcactt tggcattgat
    23821 tgcctcactc aaccattgtt caaattgggt gattgggttt gagtgacaat ctcggattga
    23881 aagcactttt ttggtgtaat cttctcgaat gtggtgcaga tccataagga ttcctttatc
    23941 ttattctaaa taatcggaca ggcacagtct gccctaccaa aattaatatt ccaacatctc
    24001 aatcaactta tcaaactccg cgaccgagcg taaatctaac caaaatttct gctgagcaaa
    24061 acgcccgata atcgggcttg ggtaggtttt aaattgttgt tccaatgcaa ttaaatcgct
    24121 ctgtttttca gcggaaatag tcactgccac cgaaggtaag gtttctgtgg gtaacgcacc
    24181 gctgccgatt tgagcgaggc ttagctcaat ttgcaaaata taagcagaat ttaaccgctt
    24241 gtgtagtgcc tgttttaatc gctctgcttt gtgttgcaag gtttcaagcg gttgagtgag
    24301 taaatgaaga gtcggcaagt tatcggttaa ttgctccgga aataagtagt ggcgtagcgt
    24361 tgcctcaagt gccgataaaa tcactttatc gcaacgtagc acccgtttta gcgggtgttg
    24421 ttgcagttgg ctaatcatcg cttggctacc gacaataatg ccggcttgcg gcccgcctaa
    24481 taatttatcg ccagagaagg aaaccaaatc taccccagcc gctacttttt gctgaacctt
    24541 tggctcagca ggcaaattaa gagcttgcat atcagttagc gaaccactgc ctaagtcggt
    24601 aatcacaggc aggttaaact ctttaccgag agccaccaat tcctcttccg acaccgaact
    24661 ggtgaacccc tcaatgtggt agttactggt gtgaactttc attaaaaagg cggtattttc
    24721 ggtgatcgcg ttgcgataat cctttaaatg agtgcgatta gtcgtaccaa cttccaccaa
    24781 tttgcaaccg gcttgagcca taatatccgg aatgcggaat gccccgccaa tttccaccag
    24841 ctcgccacgg gaaacgatga cttccttgcc ttgtgcgaag gttgctaaca tcaacaacac
    24901 cgctgccgca ttattattca caatgcaagc ggcctccgct ccggtaagta tttgcaacaa
    24961 ctcggaaatg tagttatctc ggtgcgagcg cttgcctgct tcaatatcaa attccaacgc
    25021 cacattgttt cgcattgcgt tggttgctgc ctgtatcgcc ttttccgacc acaacccacg
    25081 ccctaagttg gtgtgtaaca ccgtaccggt taagttgaac acggaacgga ttttcacctc
    25141 actcaacgct tgcacttttt gttccagcct tataaaaaac aggttttcat cggcaataaa
    25201 atccggcaag gcttgctgtt gggaaataaa ttgacgtgct tgagtaagca aactcctagc
    25261 ttgttcagcc acgctggaat atgcaaattt ttggcataat tcgaccgctt gtggcttttt
    25321 caacaagagg tcgagcgagg gaatttggcg aaatagttgt tgcataatgt gaatgattga
    25381 gagaatattt tggacaattc taataaataa agtacaatat tgccaatttt tcggaagtgc
    25441 gtcatctccg gtgaggtggc aggacttcaa atcctgttga ggacgccagc gttctcgggt
    25501 gggttcaact cccatgcact tccgccacat ttcaaaagcc gttaatcttt aacggctttt
    25561 tgcatttctg ccacattctc tctttccgtt ttgataaaaa atgagactta gctcacattt
    25621 ttttattttt ctgtttgtag taaaaattta ttaaattata attatttgga gtattttttc
    25681 acaaacaaaa tctagtccat tgactacctt ttgaacataa aaaaggaact gttatgtcaa
    25741 cactatctcg tgattctatt ttaagtcaaa ttcaaaacgg gctgattgcc tcctgccaac
    25801 cggttgatga cggccccatg gataaacctg aaattgtcgc cgcaatggca gcagcctctg
    25861 tgattggtgg tgctaaaggc attcgcattg aaggtgtcga taatttaaaa gccgctcgtg
    25921 cggcagtgaa tgtgccgatt atcggcattg taaaacgtga tttgccagat tcccctgtgc
    25981 gtattacgcc atttttagaa gatattgatg ccttagccga agccggtgcc gatattattg
    26041 cggtggacgg cacattccgc acccgcccgg tcagcattga agaggcggta aaacgcattc
    26101 acgaaaaagg ttgcttggca atggcggatt gctccaccct tgaagaaggt ttacattgcc
    26161 aacaattagg ctttgatatt gtgggtagta caatgtctgg ctacaccggt ggcgaaatgc
    26221 ctgatgagcc ggactatcaa ttagtgaaag atttaaaagc tgccggctgt aatgttatgg
    26281 cggaaggtcg ctacaacacg cctgaactgg caaaaacggc gattgaaatc ggggcggatt
    26341 acgttaccgt tggatcggca ttgacccgct tagagcatat tgtgagctgg tttgccgact
    26401 ctgtaaaatc ggctcaaaaa taggagctgc ttatgcgttg tttagcaata gacatcggtg
    26461 gcacgaaaat tgccacggca attgtgcaac acaatcaagt gtcacaacgc caacaaattc
    26521 agaccccgac tgataaacca caagctgatc aggcaggcag tttgcatcaa gcgattggtg
    26581 agataatgca acattatcaa gggcaatttg attttgtggc agttgcctca acaggcatta
    26641 tcaataaagg ggtgcttacc gcattaaatc ctaaaaattt aggcggattg gcagaatttc
    26701 cgttgaaaca aagcattgaa aaacataccg ataagccgat tgcattatta aatgacgtgc
    26761 aagcggcggt gtgtgccgaa tatttaaatg aagaccaaca agcggtcaaa aattttgtat
    26821 ttattaccgt ttccaccggc gtaggcggcg gcattatttt aaacggcgaa ctgcaaatcg
    26881 gcagtaacgg cattgcagga catatcgggc atactctagc cgatccgaac gggcctctct
    26941 gtggttgtgg acgagtcggc tgtgtcgaag cagtcgcagc agggcgtgca atagaggcag
    27001 tttccagcca atgggaaaag ccttgttcac cgaaagaggt gttcgagcga ttccgccaag
    27061 gcaacccaca agcggtcgaa ttagtacaaa aatctgcaaa agcgattgct aatttaattg
    27121 cagatttaaa aatcagttta gatatacaaa aagtggtgtt aggtggcagt gtgggcttag
    27181 cggaagggta tttaccgcag gtgtccaact atttagctga aatgccgagc gtatatcatt
    27241 gcgtattgga aaatgctcta tccgggcaag atgccggctt aatcggtgcg gcttggtggg
    27301 cggaaaacca acttaatcaa ggttttactc tttaacaaat tctaactccg gagatccaaa
    27361 tgaaaaactt aaaaggtatt tttagtgcgt tactcgtctc tttcaacgaa gacggcacca
    27421 tcaacgaaaa aggcttacgc caaattatcc gccacaacat cgacaaaatg aaagtggacg
    27481 gtttatatgt cggcggttca accggtgaaa actttatgct ttctaccgaa gagaaaaaag
    27541 aaattttccg cattgccaaa gacgaagcga aagatgaaat cgcccttatc gcccaagtcg
    27601 gtagcgtaaa cttaaaagag gcggtagaac tgggtaaata tgccacagaa ttaggctacg
    27661 acagcttatc tgcggtcaca ccgttctact acaaattcag cttcccggaa atcaaaaatt
    27721 tctacgaaac cattattcgt gaaaccggca acaaaatgat tgtttactcc attccgttct
    27781 taaccggcgt aaacatcggt gtgagccaat tcgctgaatt atttgaaaac gaaaaaatca
    27841 tcggtgtaaa attcactgcc ggcgatttct acctgttaga gcgtttacgc aaagcgttcc
    27901 cgaaccactt aatctacgca ggtttcgatg aaatgatgct accggcaacg gtattaggtg
    27961 tggacggtgc aatcggctca accttcaacg taaacggtat tcgtgcaaga caaatctttg
    28021 aattagccca acaaggcaaa atcaaagaag catttgaaat ccaaaatgta accaacgatt
    28081 taatcgaagg cattttaggc aacggtttat accaaaccat caaaggctta ttggagttag
    28141 aaggtgtgca agcaggttac tgccgtgagc cgatgacgaa agaacaaact ccggcacaaa
    28201 aagaagtgac caaacagtta aaagctaaat tcttgtaatt taagttgcaa aaggctaaac
    28261 gaaaatcacc gcttgtagta acaagcggtg attttttctc aacgacttgc aattaatgaa
    28321 gccattctgc tttttctacc acggtgtaac gtgtcaccat tatattttaa tcgcactttt
    28381 tcttggcgag tattttctga tttcaactct ctacctatat cataaaaccc tgtactccag
    28441 atattttgag taatatccgc ttttacaata atatcattga agccgttggt ctgtttgtta
    28501 tccatttgca ataccaagtt tgtgtttccg ttttggtttc tttatcatct aaaatagacc
    28561 aaaaagtcat cgggtaatca aaaacaagtt ttaacttttc tttttccggc acaaataaca
    28621 tcaaatgatc atttgcagaa tactcaggag cacttggacc acgagcagca ttattaaacc
    28681 tcacgccgaa agctcttact ttgtcgttta gaatataacg ggcggtatca attttaagac
    28741 tatctgcatt cacatcgata gaggcatcga tctccatatc ctgctcccac gattgcaata
    28801 tcgcattatc attcacacga gcaacaaaaa gcgtccactt aggtgtgttt tcgtcgtttt
    28861 gataattttc tttgctgtat ttaggctcta ttaccgcata actcactaag gtttccgttg
    28921 aattgttagg agctgtttta caggcagaag ctaaagtacg agcagaaagt ttctcttgag
    28981 aaatcttaaa gtaatgagca ataatttgat gttgttgctc agcacaaacc tgtgtgttat
    29041 taccttcatc tgcttgagtt gcaagcgtaa gtaaaagaac taagaggagt atctttttca
    29101 tatagattta tccgtataga aagataactc attctatggg gtactgactc tttttgcata
    29161 gagagaaaga aaaaaatttg gtcggaaaaa taacggcgag cctagcgaaa acaagggttc
    29221 gccgtaattt tatgggggaa gctatgtaaa aaacaaaatg agcaggatta gaaaaaaatg
    29281 agcagttttt gcgatatttt ttgcataggt ttgatcggtt ttaaaactat tttaaatctg
    29341 ttttaaacta aagagtgtcc gatgtggact acctgcccga tgatttggag gttttgagct
    29401 tcttcggcgg taatctgtat tgggcggtag gactcgttat cgctaattaa ttcaatatag
    29461 tcccattctt ttctaatccg tttaacccat acgctttccc cattctgcac aagataaatc
    29521 ttatcatcac cacgaggctc tcgtttgctg aaatctacca gcatttggtc actgtctgca
    29581 atggtagggt gcattgatgt tccatttgcc caaaacacgc cgcagttgtg ggcttttacg
    29641 ccgaggcttt gcagcaggct gtcggcgtaa ggttcttgcc cgtctggctt cgttacgccc
    29701 tcgttaaagc tgccaaagcc tgctgaaacg ttgattgttc tgaagctgtt aatcattgaa
    29761 acaatatcat cagccatttt ttctagtcgc tcaaactctc ttgccacaat actggtattc
    29821 tctgattgtg gtttgctttc cttacctgtt gccagccatt caaggctcac atttgacacc
    29881 tctgctattt ttacaagatt tgaacgagat ggatctgcct ctccttttac ccacctagat
    29941 agtgatgctt gggctacccc cactgctctt gcaaactcac tgtaattgtc cttaaagcta
    30001 ttttttgcaa tccatttcat tctttctgag aagctctcat cgtaaatgtt tggtttactc
    30061 atcttttacg tttccattaa taaagtttta ctcgtaaaac atactcataa accattgatt
    30121 tttaagtt
//
LOCUS       DANLXQ010000011        30074 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.011, whole genome shotgun sequence.
ACCESSION   DANLXQ010000011 DANLXQ010000000
VERSION     DANLXQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 30074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30074
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.011"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            42..1031
                     /gene="dusB"
                     /locus_tag="PXD94_000390"
     CDS             42..1031
                     /gene="dusB"
                     /locus_tag="PXD94_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL6285292.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFETGLSSPLSIDKKYQLMFKHIEDLHQFYGEEKGYR
                     IARKHVGWYTEKLNPESNFKRTFNTLSSTKEQLYALEDFVKSIHLESQLRKVG"
     gene            1036..1332
                     /gene="fis"
                     /locus_tag="PXD94_000391"
     CDS             1036..1332
                     /gene="fis"
                     /locus_tag="PXD94_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL6285293.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(1437..4223)
                     /locus_tag="PXD94_000392"
     CDS             complement(1437..4223)
                     /locus_tag="PXD94_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL6285294.1"
                     /translation="MKYHHFRYSPVALTVLFALSHSYGAATENKKIEENNDLAVLDEV
                     IVTESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSG
                     YAIRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEY
                     GSGALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALV
                     IATHRHGKETKIHSEANKLKHNIRRITGFENRYDLYPNSAQNAPGGSFFIVEDTCPTL
                     DCTPRARVKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLD
                     YKSNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINLSLRNYVYEG
                     DNILDGLVFKPRIPYGLRYSHVKFFDERHHKRRLGFTYKYKPENNRWLDSIKLSADKQ
                     DIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDKALNA
                     GQGVFKQTHKLNLGLGFDRFNSLMDHGDMTAQYTKGGYTSYRGRGRLDNPYIYRRDPR
                     SIETVSLCNNTRGDILNCEPRKIKGDSHFVSFRDLVISEYVDLGLGVRFDQHRFKSDD
                     PWTLSRTYRNWSWNGGITLKPTEFVSLSYRISNGFRVPAFYELYGKRDHIGLKDNEYV
                     QRAQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKSHCFY
                     NYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLTDSRLDSVND
                     PILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVAGTRHHGIHRVDLGGKL
                     TGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNYTRFA
                     APGRNFSLAFEMKF"
     gene            complement(4317..6074)
                     /locus_tag="PXD94_000393"
     CDS             complement(4317..6074)
                     /locus_tag="PXD94_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL6285295.1"
                     /translation="MFKLKSSFVLLNAALLAACSSNGGSFDVQSAKVESQTQTTPKKP
                     SLQDDNSNARRTVSASETEALLQPGFGFSAKIPRRNLFPQGKEDVAPIGDIKEITGDL
                     TKIPYEEEVKAYGSSADGFSHTHDRNHKLYTRDFNFVRSGYVLHSGAKPEIKPKEILR
                     TGAHGYVYYLGIEPAKAIPTQKATYKGYWDFTTDARKGRDSKYFSNSAGINIGATPEN
                     SYDINVDDSEKPMGHTGEFTADFANKTLTGTLVRNGYVSRSKEQKITTIYDIDAKIKG
                     NRFSGKANAKNTDDPYFGKSSTTLEGGFFGGKAQELAGKFLADDKSVFVVFAGKRDAK
                     KDDSESAFDAFKIKLKDLNKSEMDTFGNATHLIINNKQIPLIAEGKKSFAEMKFYDLV
                     TRTIDDKTYRVSVCCNNLDYVKFGTYSEGNNSDTAHQYLVGERTAVADLPTGTVKYRG
                     TWDGVMYSKSGSAGAESPSNSESGTRSLFDVDFVNKKINGKLIANDGVEERPMLTLEG
                     NLKGNGFAGTAKTGNSGFNLDPKSTNGGTVVHINTQFEGGFYGPKATELGGIVHSAET
                     DKDRVSITFGGKRQIQIEK"
     gene            complement(6495..7142)
                     /gene="rnt"
                     /locus_tag="PXD94_000394"
     CDS             complement(6495..7142)
                     /gene="rnt"
                     /locus_tag="PXD94_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL6285296.1"
                     /translation="MEQTTEQIDYKLLKHRFRGYLPVVIDVETAGLNAQTDALLELAA
                     ITLKMDEQGYLVPDQQCHFHIAPFEGANINPESLKFNGIDIDNPLRGAVAENIAIPEM
                     FKMVRKAMKEQGCQRAVIVAHNAAFDQGFLQAAIKRINAKRDPFHPFAMFDTATLAGF
                     MYGQTVLVKACQVAKIPFDGKQAHSALYDTERTAELFCAMVNRLKDLGGFPPLSD"
     gene            complement(7227..7634)
                     /gene="gloA"
                     /locus_tag="PXD94_000395"
     CDS             complement(7227..7634)
                     /gene="gloA"
                     /locus_tag="PXD94_000395"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL6285297.1"
                     /translation="MRILHTMLRVGDLERSIKFYTDVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDKYELGTAYGHIALGVDDIYKTIEDVRAAGGKITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKDAKAALGN"
     gene            7724..8539
                     /locus_tag="PXD94_000396"
     CDS             7724..8539
                     /locus_tag="PXD94_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285298.1"
                     /translation="MAHDHDSPSKLEISTHAALPMVLMLFIEMALNFYSAPKNSIFIS
                     PYLLAFFVTSLISFLVLWKGEICRGQASRLSFILPFLLIFAVGNFLYSIGFTPKHNPM
                     AVASIAAVFACLIYFRLPEDQTLYNTLMYCGLAISSIGIIQYLAIYWFELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDVFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFIIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLAILYPFANVV"
     gene            8543..9289
                     /locus_tag="PXD94_000397"
     CDS             8543..9289
                     /locus_tag="PXD94_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285299.1"
                     /translation="MKRALKFFGIATVLSVVAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLSVDKLDIDYRCLALFP
                     QNEHSNSDTSLNALLAVIPDGEATIHALNWKNLPTDFNPRLQRLFEQTAIKLSLSKGE
                     IQATAAQQAVKFAANFANGRLNADLSYQPNEKEQHNLRISAEIEDNFTQLPPTISAEY
                     DWQLSYEIIANKALQKGALFSVGKKMPHKS"
     gene            9475..11250
                     /locus_tag="PXD94_000398"
     CDS             9475..11250
                     /locus_tag="PXD94_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HDL6285300.1"
                     /translation="MPLKTAIRISLLSQNSWGKGNIVISNSDGEITEKGLNLPLRLTG
                     NIKHGNFILYSSVPLDFQGNFDDLSVKFLPSSLLRLTGKERYLTIEDLRFPLAGIKID
                     KHGIHGRLHAILRGQSPDFNNIEFHLDGQAQNFKAGALDFFQDPKDANAVKDSWKWRI
                     WGASILKAFNSKVNLSGRGQWHKQLVRLEELKGDLATVKLADTTISKITLNNTQAIRF
                     NVKKFTLDGAVMLQSPEIAFSYGGVLPKPRVNLAFDGEVEKLRFKGAVNTTELGPLKL
                     FARRQLSQDASQIIGKLYWPEQSAQGFQPLFPFRSQWVINNGTIRGETAFSAGSKNGL
                     IAGGHLAIRNGGLSLPNGEAKGIEFSLPYRYQNGRFHFGAKKPLDVKIAQIKLGDLAL
                     DNASMKLNGYYPYTKAKPLNLNQLSFDLFGGKLNVKRFALPQTELAYLNLERIDFEQI
                     LQFMQYQQIDLKGKANAILPFWLSGKPCYICDGLLTQAETSYLTFTPELLSEMQKSGY
                     TEQILLHLVDKSTINDFRSLINVGPKGDLVLDGKLKLSSNQNKSKVNLNYNHRENLFD
                     LWHLINYGSQFEQKLENYLYQKLER"
     gene            11268..11462
                     /locus_tag="PXD94_000399"
     CDS             11268..11462
                     /locus_tag="PXD94_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDL6285301.1"
                     /translation="MKPATFYFAIFLLNLTACTPKVQLETPSEGITINMNVVVDHRID
                     VKMDEKSRAVITKSENKTKN"
     gene            complement(11459..11932)
                     /gene="folK"
                     /locus_tag="PXD94_000400"
     CDS             complement(11459..11932)
                     /gene="folK"
                     /locus_tag="PXD94_000400"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL6285302.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKFANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
     gene            complement(12135..13466)
                     /gene="pcnB"
                     /locus_tag="PXD94_000401"
     CDS             complement(12135..13466)
                     /gene="pcnB"
                     /locus_tag="PXD94_000401"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL6285303.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLREDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPTIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAVLLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTAKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSYDAMMLAANDVLAESCN
                     AIALHRRHTSVIRDIWALQFQMMKRSGNRPQQTLEHVKFRAGFDLLVMRAEIEGGDLV
                     ELSAWWHEYQFSNEGQRTELLKSVRHLGNVPSEKKKTRRRRTFKKKPKVNG"
     gene            complement(13711..14157)
                     /gene="dksA"
                     /locus_tag="PXD94_000402"
     CDS             complement(13711..14157)
                     /gene="dksA"
                     /locus_tag="PXD94_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL6285304.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDEYGYCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            complement(14407..15360)
                     /gene="thiB"
                     /locus_tag="PXD94_000403"
     CDS             complement(14407..15360)
                     /gene="thiB"
                     /locus_tag="PXD94_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL6285305.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTIEFTPPTTETIQKWLADWSKSW"
     regulatory      complement(15404..15547)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15831..16817
                     /locus_tag="PXD94_000404"
     CDS             15831..16817
                     /locus_tag="PXD94_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL6285306.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDMIGGLPYRDELDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     gene            complement(16871..17290)
                     /locus_tag="PXD94_000405"
     CDS             complement(16871..17290)
                     /locus_tag="PXD94_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL6285307.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(17368..18123)
                     /gene="kdsB"
                     /locus_tag="PXD94_000406"
     CDS             complement(17368..18123)
                     /gene="kdsB"
                     /locus_tag="PXD94_000406"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL6285308.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            complement(18133..19086)
                     /gene="trxB"
                     /locus_tag="PXD94_000407"
     CDS             complement(18133..19086)
                     /gene="trxB"
                     /locus_tag="PXD94_000407"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL6285309.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            19253..20323
                     /gene="dhaK"
                     /locus_tag="PXD94_000408"
     CDS             19253..20323
                     /gene="dhaK"
                     /locus_tag="PXD94_000408"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL6285310.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            20344..20970
                     /gene="dhaL"
                     /locus_tag="PXD94_000409"
     CDS             20344..20970
                     /gene="dhaL"
                     /locus_tag="PXD94_000409"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL6285311.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            20980..21387
                     /gene="dhaM"
                     /locus_tag="PXD94_000410"
     CDS             20980..21387
                     /gene="dhaM"
                     /locus_tag="PXD94_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL6285312.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAVKIMQAIESVFSPDGVIVFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEG
                     AFAAVVAASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(21427..22272)
                     /locus_tag="PXD94_000411"
     CDS             complement(21427..22272)
                     /locus_tag="PXD94_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL6285313.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(22316..22642)
                     /gene="glpE"
                     /locus_tag="PXD94_000412"
     CDS             complement(22316..22642)
                     /gene="glpE"
                     /locus_tag="PXD94_000412"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL6285314.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFENVYSVKGGFEAWERNGL
                     PIETSY"
     gene            22793..23251
                     /gene="exbB"
                     /locus_tag="PXD94_000413"
     CDS             22793..23251
                     /gene="exbB"
                     /locus_tag="PXD94_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL6285315.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            23288..23725
                     /gene="exbD"
                     /locus_tag="PXD94_000414"
     CDS             23288..23725
                     /gene="exbD"
                     /locus_tag="PXD94_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL6285316.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            23741..24652
                     /locus_tag="PXD94_000415"
     CDS             23741..24652
                     /locus_tag="PXD94_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL6285317.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREELQEQEKPKDKPKERPKHHHKHIKALERGPEI
                     KQGIVAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVA
                     LQRALQRKANNSYPQREKMMRKTGTVTIKFTITSSGSITNVSVLNSSGNSNLDNAAVR
                     SAEGLSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(24721..25602)
                     /gene="cysZ"
                     /locus_tag="PXD94_000416"
     CDS             complement(24721..25602)
                     /gene="cysZ"
                     /locus_tag="PXD94_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL6285318.1"
                     /translation="MSSVFSEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTV
                     LMVGLIWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAP
                     FNALLAEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLP
                     VVGQTVAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSL
                     FTMIPFVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTET
                     RTGEVSPNVRNGDIRKQ"
     gene            25714..26616
                     /gene="zipA"
                     /locus_tag="PXD94_000417"
     CDS             25714..26616
                     /gene="zipA"
                     /locus_tag="PXD94_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL6285319.1"
                     /translation="MELHIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIA
                     VPPQNNKMSNSIPTSLQPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQ
                     QPEQIQPIQSVAEPTVSTYGQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQE
                     TQSAEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPI
                     IFSAANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGG
                     FLYDDNQQPFGEESRQRYMQRVMH"
     gene            26681..28738
                     /gene="ligA"
                     /locus_tag="PXD94_000418"
     CDS             26681..28738
                     /gene="ligA"
                     /locus_tag="PXD94_000418"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL6285320.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGEEI
                     TANIRTIRNIPLKLNTTNPPARLEVRGEVFMPQSGFNELNEKALAKGEKTFANPRNAA
                     AGSLRQLDPKITRQRPLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLV
                     QGKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQ
                     EEMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVI
                     IRRAGDVIPQITGVMLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEA
                     QRKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKS
                     AQNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQ
                     DVGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLT
                     QLTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIEL
                     TQH"
     gene            complement(28801..30069)
                     /locus_tag="PXD94_000419"
     CDS             complement(28801..30069)
                     /locus_tag="PXD94_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL6285321.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
BASE COUNT         9118 a         6468 c         5820 g         8668 t
ORIGIN      
        1 ctggttaaaa ttacttcaaa tttgttaata aaaggttaaa aatgcaaata ggacaatatg
       61 caatcaaaaa tcgtattttc cttgcaccta tggctggaat cacagatcag ccttttcgcc
      121 gcttatgtag ccaacttgga gcaggtttaa cattttcaga gatgatgtct accaatcctg
      181 atgtatggca tactgagaaa tcacgattgc ggttagcaca ccacgatgaa ataggcttaa
      241 atgccgtaca aattgcaggt tctgatcctg ttgaaatggc aaaggctgca aagataaatg
      301 ttgagtatgg tgccgatatt attgatatta atatgggttg cccggcaaaa aaggtgaata
      361 aaaatatggc aggctctgct cttttacgta accccaaatt agtcgctcaa atattagatt
      421 ctgtagtcaa tgccgttgat gtccctgtga cactaaaaat tagaacaggt tgggatcagg
      481 aaaatagaaa ttgtctgcaa attgcgaaaa ttgcagaaca agccggcata aaagctctca
      541 ctattcacgg gcgtactcga agctgtttat ttaatggcga agctgaatat gaaagtatta
      601 aggcagtaaa agaagcaatt tctatcccag tgatagcaaa cggagacatt gactctgcgg
      661 aaaaagcaaa gtttgttctc gactatacca aagccgatgc aatcatgatt gggcgcgcta
      721 gctttggaaa tccttggata tttaaggaaa ttaatgattt ttttgaaact ggattatctt
      781 caccattatc aattgataaa aaataccaat taatgtttaa acatattgag gatttgcacc
      841 aattttatgg tgaagaaaaa ggttatcgta ttgcacgaaa acatgttggt tggtataccg
      901 aaaaattaaa tccggaatca aattttaaac gcacttttaa tacactaagt tccacaaaag
      961 agcaacttta tgcattagaa gattttgtta aatcgattca tttagaatct caattaagaa
     1021 aagttggata aaaaaatgtt agagcaacaa cctacacaaa acccattaac agtaacaatg
     1081 ttaaatgcac aagcgcagca ggttaataaa ccacttcgcg ataatgtgaa agcagcatta
     1141 aaaaactatt tatctcaatt aaatggtgaa gatccaacag aattatacga attagtatta
     1201 tctgaaatcg aacacccaat gttagatatg gtgatgcaat atactcgcgg caatcaaact
     1261 cgtgcagcaa caatgctagg cattaaccgt ggcacattac gcaaaaaatt aaaaaaatac
     1321 ggtatgggtt aatctaaccg accgttaaaa cacgatttta attcgtatgt aattagttag
     1381 agccaacaaa aaacgctgaa tatactattc agcgtttttc tattttattt tgccgtctaa
     1441 aacttcattt caaatgctaa actgaaattt ctccccggag cggcaaatcg agtgtaattg
     1501 ctaccccggt cttggtttac tgcattcaca ccggattggc gcactgattc ccaagtggaa
     1561 tatttacgat tagtcacatt ataaattcct ccacgtaagg tgtagttttt ataattgatg
     1621 taaccggtaa tatcatgggt gtaccaagaa ccggtcagtt tgccacctaa atcaacgcga
     1681 tgtatgccgt gatgacgtgt gcctgccacc tcatcggcgt ttttggcttt agaataggtg
     1741 gtagtaatgc caattcccca tttttcttct gggtgatcgt agccgaatcc aagcacatag
     1801 cgtgctggct gaatcgcatc tagaatagga tcgtttacgc tatcgagtct tgagtcggtt
     1861 agtttccgct cttttacttt tacacggtta taagcaactg atgaataaaa accatctggc
     1921 agcatagata aaataccgtg taagtcaaat ttagcgacta aatttatccc gtttagtgct
     1981 acatcttgaa tattatggta gttatagaaa cagtgtgatt tttgtattat tcttttacat
     2041 gctgtcgcaa tcatattttt atagttatta cggaaatagc tcacatcaag gtaaccaaat
     2101 tgacctttaa agctaactcc aatctcatga ttagtcgatt tttctggctc taactggtgg
     2161 ctacgttgcg cgcgttgcac atattcgtta tctttaagcc caatatgatc acgtttacca
     2221 taaagttcat agaatgcagg cactctaaaa ccgtttgaaa tgcgataaga aagcgataca
     2281 aactctgttg gtttaagcgt aatcccacca ttccaagacc aatttcgata agttcggcta
     2341 agtgtccacg gatcatcaga tttaaatcga tgttgatcaa aacgcacccc taatcccaaa
     2401 tccacatact cgcttatcac tagatcgcgg aagctaacaa aatggctatc gcctttaatt
     2461 ttacgcggtt cacagtttaa gatgtcgccg cgtgtattat tacacaaaga taccgtttca
     2521 atactgcgtg gatcgcggcg ataaatatat ggattatcta aacgccctct accgcggtag
     2581 ctggtataac cgcctttggt atattgggca gtcatatccc catgatccat aagcgaatta
     2641 aatcgatcaa agcccaaccc taaattcagt ttgtgggttt gcttaaatac gccttgacca
     2701 gcatttagcg ctttatcaaa ttctaaatga atgacacgat gcttttcttg gtaattatta
     2761 cgctcagttc gatacataga ccaagattta tccaaagtcg ggcggcaatt tttatctacc
     2821 acaggataat cgctacaatg caagcgatgt agccggctat atagttcaat atcttgttta
     2881 tccgcactga gtttaatgct atccaaccag cgattattct ctggtttata tttataggtg
     2941 aatcctaaac gacgtttgtg gtgacgttca tcaaaaaact tcacatggct atagcgcaac
     3001 ccataaggga tccttggctt gaacactaag ccatctaaaa tattatcccc ttcataaaca
     3061 tagttcctaa gtgataagtt aatatcgtct tttgtatagt aagctggcgt ttgcatatca
     3121 cggatatcgt agcgttgttt tgtatcttct aagattgcgc caagataatg agacgagtta
     3181 aagtgatagc caaacttcat aaaaacagaa ttactcttgt aatctaaagg gtttggtgca
     3241 atgcgatctt taccggtata ttcttgggct gagagctgct cagtgcgata aggaatctgc
     3301 tcaagctgtt tgcgctcttc aggcgtatat tccggaaatg ttctcactgg gaaattatcg
     3361 cggttcaact taacccttgc acgaggagta caatctaatg ttgggcaagt atcttccaca
     3421 ataaaaaaag atcctccagg agcattctgt gcggaatttg ggtaaagatc gtagcgattt
     3481 tcaaagccgg ttatacgccg aatattatgt tttaatttat ttgcctcgga atgaattttg
     3541 gtctctttac cgtgtcggtg agttgcaata acaagtgctt caaaaccacc cgcctcacca
     3601 gccgctgcga tagactgtaa aaaatggcta tttttgctgg cataagaggt cttactatct
     3661 aagccccaat gctgcccctc tttaataata tcctgcgcat ctttagtacg aaaaccaata
     3721 gcaccaccta gcgcaccaga gccatattcc gcagaacttg ctcctttgct taactcaatt
     3781 gaacgaatgt tttcatactc aatctcatta attgcaccac cattagcatc tgaacctagc
     3841 gtatgataat gctgcgcttg tggtagccca tcaacaagta agctgacacg gtttttatct
     3901 acacctcgaa tggcatagcc actacttgca ccgcgacctt gttccaccac cgaaatacca
     3961 gggtcatagc gagttaaatc acgaatacca agaatttgat ttttactcat ttcgtgataa
     4021 tttttcacta ctttccccaa gccagttact tcgttttgac gttcgtgagc ataatggctc
     4081 tctgtcacaa taacttcatc cagaacagct agatcgttat tttcttctat ttttttattt
     4141 tcagtcgcag caccgtatga atgagaaaga gcaaataaca ctgttaaggc aacaggtgaa
     4201 tagcgaaaat gatgatattt cattatcatt agatagggtc tcctttattt aaattttgca
     4261 aatttttata aaaaattgcc cgcttgctat ctacaaccag caaaggggaa ttatgattat
     4321 ttttctattt gtatttgacg ttttccgccg aatgtaatac tgactctatc tttatccgtt
     4381 tctgcactat gtacaatacc acctaattcc gtcgccttag ggccataaaa gcccccttca
     4441 aattgagtat ttatatgcac taccgtgcca ccattcgtac ttttcggatc aagattaaaa
     4501 ccagaattgc ccgttttggc tgtgcctgca aaaccattcc ctttcagatt gccttcaagt
     4561 gtcagcattg ggcgttcttc aacaccatca ttagcaatca gcttgccatt aattttttta
     4621 ttgacaaaat ctacatcgaa tagtgaacga gtaccacttt cgctgttact tggcgattca
     4681 gcccctgccg agccagattt actgtacatt accccgtccc aagtacctcg atattttact
     4741 gtccctgttg gcaaatctgc cacagctgta cgttctccta ctaaatattg gtgagcagta
     4801 tcactattat ttccctcgct ataagtccca aatttgacat aatctaaatt attacagcag
     4861 actgaaactc gatacgtttt atcatcaata gtacgggtaa ccaaatcata aaatttcatc
     4921 tcggcaaagc tttttttgcc ttccgcaata agtggaatct gcttattgtt aataatcaaa
     4981 tgtgtcgcat tcccgaaagt atccatctca gatttattta aatctttaag tttaattttg
     5041 aaagcatcaa aggcagattc actatcatct tttttagcat ctcgtttgcc agcaaaaaca
     5101 acaaataccg acttatcatc agctaagaat ttaccggcaa gttcttgagc cttcccacca
     5161 aaaaatccac cttcaagtgt cgtagagctt ttaccaaaat aaggatcatc ggtatttttt
     5221 gcgtttgctt taccagaaaa gcgattacct ttaatttttg catcaatatc gtaaattgtt
     5281 gtaatttttt gctctttgct acgactaaca tacccattac gaaccaatgt tccagttaaa
     5341 gttttattag caaaatcagc cgtaaattct cctgtatgcc ccattggttt ttcagaatca
     5401 tcaacattaa tatcgtaact attttccggt gtggcgccaa tattgatgcc tgcggaatta
     5461 gaaaaatatt tactatctct cccttttctc gcatcggtag taaaatccca atatccttta
     5521 taagtagctt tttgagtagg tattgctttt gccggctcta tacctaaata gtaaacatac
     5581 ccatgtgcac ctgttctcaa aatttcttta ggctttattt caggttttgc accagaatgc
     5641 agaacatagc cagaacgaac aaaattaaaa tctctagtat acaacttatg atttctatca
     5701 tgagtatggc taaatccatc agcactacta ccgtacgctt taacctcttc ttcatacgga
     5761 atttttgtca gatctccagt aatctctttt atatcaccaa taggggctac atcttccttc
     5821 ccctgcggaa agagattacg acgcggaatt ttggctgaaa aaccaaaccc cggctgcaat
     5881 aaagcttcag tttcagaagc gcttactgta cgtcttgcgt tactattatc atcttgtaaa
     5941 cttggctttt tgggggtagt ttgcgtttga gattcaactt tggcagattg aacatcaaag
     6001 cttccaccat ttgaggaaca agcagcaagt agcgccgcat taagcagtac aaaactactt
     6061 ttaagtttaa acataatgtc tccttttaat ttataaaatg ataatcatta taatttatta
     6121 tataaactaa tgtaaaggtt tgaactttat tagtttcagg atcatgttaa tcatttcaag
     6181 gtggaaacgc ttgtttttag attagataaa aaataaggag ggtcgaaata gcgggtatac
     6241 agaaagtgct tatagccaaa aaatagtcta ttgttattga atacaagcgg tctattttgt
     6301 cacatttttg ccaaatttgg ttaaaaaacc aaccgcttgt aatcttaact gaagatagat
     6361 agttttttat tttttctaca actctacagg gctggattta ttgcagaatg gtacttgcca
     6421 ctaactatca tcactcccag gataatttat agaaacaata cgagaaattg tgttaataaa
     6481 tgaaatgaat aacgttaatc cgataatggt gggaaaccac ctaaatcttt taaacgattg
     6541 accattgcac agaaaagttc tgccgtgcgc tcggtatcat acaacgcaga atgcgcttgc
     6601 ttaccgtcaa acgggatttt tgccacctgg caggctttta ccaacacggt ttgcccatac
     6661 ataaaaccgg caagggtggc ggtgtcaaac atcgcgaaag ggtgaaaggg atctcgcttg
     6721 gcattaattc gtttaatcgc agcctgtaaa aagccctgat cgaacgctgc attgtgggca
     6781 acaatcaccg cccgttggca gccttgctcc ttcattgctt tgcgaaccat tttgaacatt
     6841 tccggaatcg caatattctc cgcaaccgct cctcttagag ggttgtcaat atcaatgccg
     6901 ttgaatttta atgactcagg gttaatattc gccccttcaa atggcgcaat atgaaaatgg
     6961 cattgttgat ccggcactag ataaccttgc tcgtccattt ttaaagtaat tgccgccaat
     7021 tccaataagg catcagtttg ggcatttaag cctgctgttt ccacatcaat cacaaccggt
     7081 aaatagccac gaaaacgatg ctttaataat ttataatcta tttgttccgt tgtctgttcc
     7141 atttatctat caacctaaaa tacaagcggt ctgatttgca aaatttttta caaatccgac
     7201 cgcttgaata ttcatattcg caaaaattag tttcctaacg ctgctttcgc atctttattc
     7261 tcaataaatt cgattttata accgtccgga tcttcagcaa aggcaatcac agttgttcca
     7321 cccaataccg ggcccggctc acgggtaatt ttaccgcctg ccgcacgcac atcttcaatg
     7381 gttttgtaaa tatcatctac gcctaacgca atatggccat aagccgtgcc tagctcatat
     7441 ttatccacac cccagttata agtcagctca atcaccgcac tttcgctttc atcggcatag
     7501 cctaaaaatg ctaacgaata cttatactgc tcgttctcgc ttctacgtaa cagacgcata
     7561 cctaacacat cggtataaaa cttaattgag cgctctaaat ctccaactcg taacattgta
     7621 tgtaaaattc gcatattttc ttccccctga agttaaaaaa cgacccctat tatggcataa
     7681 tcttggctta aaatcatttt ctttatacct tttgggatta tctatggctc acgatcacga
     7741 ttcgccgtca aaacttgaaa tttcaaccca cgctgcactg ccaatggtat taatgctgtt
     7801 tattgaaatg gctcttaact tctactctgc accgaaaaat tcaattttta tttctcctta
     7861 cttattggcg tttttcgtga cctcattaat cagtttttta gtgctatgga aaggcgaaat
     7921 ctgccgaggg caagccagcc gtttaagctt tattttaccc tttttactta ttttcgccgt
     7981 agggaatttc ctttattcca taggctttac gccaaagcat aacccaatgg cggtggcaag
     8041 cattgctgca gtttttgcct gcttaatcta cttccgcctg ccggaagatc aaaccttata
     8101 taacacattg atgtattgtg gtttggctat atcaagtatt ggtattatcc aatatttagc
     8161 catttattgg tttgaactgc cttcattgtt taacgggatt cgagccaaca atttcgccca
     8221 agtgctgtta ggcattttat tagccggctg gtatttaatg ttagcgaaaa gccgtttaga
     8281 tgttttcctc aaactgttgg tgaaaaccgc ccttgtggtg ctgattctca attattgctg
     8341 gacagctttt gtgctttatc aacatctaca aattatgccg gatatgccat tgctttcatt
     8401 tatcatctat tttgcagcac aatttgtgat tttattgatg ttggcgtggt tattacttgg
     8461 aaaaaacatc aaaaatccga ccgcttggac agttgctacg ctgttagcga ttttataccc
     8521 tttcgctaat gtagtctgat ttatgaaacg ggcattaaaa tttttcggca ttgcaaccgt
     8581 tctatctgtt gtggcaatct actatttttt aatgcccagc cactatcttg ccacgccgat
     8641 taactggatt ttgccgaaag cgtggaaagt tcaactccca caaggcttaa acctgaattg
     8701 gcaaggctca tcacttgctc aatttcaact ctcttatcga gattgcccgc tactaagtgc
     8761 cgataaaacc gatttgggtt ggattgagaa aaaccatctt tctgtcgata aattggatat
     8821 tgattaccgc tgccttgcct tgtttccaca aaatgaacac agcaattccg acacttcact
     8881 caacgcattg ctggcggtca ttcctgatgg cgaagcgact atccacgccc ttaactggaa
     8941 aaatttgcct accgatttca acccaagact tcagcggctt tttgagcaaa cagctattaa
     9001 actctcactt tcaaaaggtg aaattcaggc aactgccgct caacaagcgg tcaaatttgc
     9061 agcaaatttt gcaaatgggc ggttgaatgc cgatttatca tatcaaccga atgaaaaaga
     9121 acagcataat cttaggattt cagcagaaat tgaggacaat tttacccaac tgccgcccac
     9181 tatttccgct gaatatgatt ggcaattatc ttatgaaatc atcgccaata aagccttgca
     9241 aaagggcgct ctattctcag ttggcaaaaa aatgccgcac aaaagttaga aggcaactgg
     9301 caggttgaac ttgccgaaag tgaaaataac aaactggatt tcccttttct gtttgatggg
     9361 gaaagcctag aaattcaaca agggcggttt gattggcact ttacccaaca atttccactg
     9421 caagggtttg ttagcaccaa gctcacaccg aaatcattca accaaaacgg ctttttgcca
     9481 ctcaaaacgg cgattcgtat cagcctgctt tcccaaaaca gttggggtaa aggcaatatt
     9541 gtaatctcca attcagacgg agaaataacc gaaaaagggc tgaatttacc gcttcgtttg
     9601 acgggtaata tcaaacacgg caattttatt ctttacagta gcgtgccact ggattttcaa
     9661 ggtaattttg acgatttaag cgtaaaattt ttgcctagct ccctacttcg tttgaccgga
     9721 aaagagcggt atttaaccat tgaagattta cgttttccac ttgccggaat taaaattgac
     9781 aagcacggta ttcacggacg actgcacgca attttaagag ggcaaagccc tgattttaac
     9841 aatatcgagt tccatttaga cggacaagca caaaatttta aggctggagc cttagatttt
     9901 ttccaagatc ctaaagatgc taacgcagta aaagacagtt ggaaatggcg aatttgggga
     9961 gcatctatcc tcaaagcctt caacagtaaa gtgaatttaa gcggcagagg gcaatggcat
    10021 aaacagcttg tgcggttgga agaactcaaa ggcgatttag ccaccgtaaa gttagcagat
    10081 acaaccattt ctaaaatcac gcttaacaat acgcaagcta ttcgatttaa tgtcaaaaaa
    10141 ttcacccttg acggagcggt aatgttgcaa tcacctgaga ttgccttcag ttatggcggt
    10201 gtgctaccca aaccacgagt gaatctcgcc tttgatggtg aagtcgaaaa actacgtttt
    10261 aaaggagcgg tgaataccac cgaactcggc ccactcaaac tgttcgcccg ccgccaatta
    10321 agccaagatg ccagccaaat tatcggcaaa ctttattggc cggagcaatc tgctcaaggc
    10381 ttccagccgc tttttccgtt tcgtagccaa tgggtgatca acaacggtac aattcggggc
    10441 gaaacggcgt ttagtgccgg cagtaaaaac gggctgattg ccggtgggca tttagccatt
    10501 cgtaacggcg gtttatcttt acctaatggc gaagcaaaag ggattgaatt ttcgcttccc
    10561 taccgctacc aaaacggacg ctttcatttc ggagccaaaa aaccgcttga tgtaaaaatt
    10621 gcccaaatca aactggggga tttagcatta gataacgctt caatgaaact caacggttac
    10681 tacccttata ccaaagctaa gccattgaat ttaaatcaat tatcctttga tttgtttggc
    10741 ggtaaactga atgtaaaacg ctttgcctta ccacaaaccg aactggctta cctgaattta
    10801 gagcggattg actttgagca aattttgcaa tttatgcaat atcagcaaat tgatctcaaa
    10861 ggcaaagcca acgctatctt gccgttttgg ttaagcggaa aaccttgcta tatttgcgat
    10921 ggcttactga cacaggcgga aacttcctat ttaaccttta cccctgaatt gttgtcagaa
    10981 atgcagaaaa gtggttacac cgagcaaatt ttgttacatt tagtagataa aagcacgatt
    11041 aacgattttc gcagtctgat taatgtcggg ccgaaaggcg atttagtatt agacggaaaa
    11101 ttaaaattat cttccaatca aaataaatca aaagtgaatt taaactataa tcaccgagaa
    11161 aatctgtttg atttgtggca cttaattaac tatggctcac aatttgaaca aaaattagaa
    11221 aattacctct atcaaaaact ggaacgatga taaagaatgg aataaatatg aaaccggcca
    11281 cattttattt tgcaatattt ttactgaatt tgaccgcttg tacgccgaaa gtacagcttg
    11341 aaacgccaag tgaaggcatt acgattaata tgaatgtggt cgttgatcac cgcattgatg
    11401 tgaagatgga cgagaaatcc cgagcggtga tcaccaaaag tgagaacaaa acgaaaaatt
    11461 agctgaagat aatcatttga tgatctttaa attgtgcata aatatcggct aatttttccc
    11521 ctgtcggtag tactaaatca ggcgttaaat caaacatcgg cacaatcaca aattcccgat
    11581 ttttcatctc gatatgcggc acgttcaaac gctcggtttg aatttgctga ttgccgtaca
    11641 aaataatatc cagatctaaa gttctctccc cccaacggcg tacacgcact cttccctgct
    11701 cattttcaat gctttgcaat ttatccagca atgcagtggg cgataggtcg gtactaaatt
    11761 ttgctaccgc attgacataa tcaggctgat cttgcggtcc gacaggcttt gagccgtaga
    11821 acgggctgat ttcaaactca tttgcaaatt tttttaattt ttcgaccgct tgtttaagct
    11881 gctcaagcgg attctcaagg tttgatccta aggcgatata gacggtttgc ataaaatata
    11941 cttacaaata taacaataaa ctctctcccc ttgtgggaga gagaccgctt atgagcgttc
    12001 agcgaaataa gcagagagag gggacaagcg gtaactttta ttaaattttt tacacatttc
    12061 ccctctccct gatttttctt ctgaacgaag aaaaatcgct ccctctccca caaggggaga
    12121 gggaaatata ttaattatcc atttactttc ggttttttct taaaggttct acggcggcgt
    12181 gttttctttt tctcgctagg cacattgcct aaatgacgaa ccgatttcag caattcagta
    12241 cgctgacctt cattgctaaa ttggtattcg tgccaccaag cggaaagttc gaccaaatcg
    12301 ccgccttcaa tttctgcacg catcactaat aaatcaaacc ctgcacggaa tttgacgtgt
    12361 tcaagggttt gttgcggtcg gttgccactt cgtttcatca tctgaaattg caatgcccaa
    12421 atatctcgaa tgactgaagt gtgtctgcgg tgcaacgcaa tcgcgttgca actttccgcc
    12481 agcacatcat ttgctgccag catcattgca tcgtagctgt tgaagccacc ttcgttttta
    12541 agctgctcca ttttctcccg gagcggatac caaagtagtg cagcaaataa aaacgcagga
    12601 tttaccggta atttatcggc aattcgctca tcagttgaat ttaacgcttt ggtaatcatt
    12661 ctttcggcat aggaatcacc tttggcagta aaaaatgccg gcaataccgg aaacaagaat
    12721 tgccatagtt catattcacg caaaagctga taagttttca gccctgcacc ggtttgtaat
    12781 aatttgagtg attcgtcaaa caaacgggct gccggaatgt tggttaacaa caccgccata
    12841 tcccgaattg gggaggccgt ggctttatcc aagaacatat ccaacttcgc cataaaacga
    12901 acggctcgca acattctcac cggatcttct tggtaacgaa tagtaggctc tccgataagc
    12961 gaaagtttac ctgctcgtaa gtcttcaata ccgttgaaga aatcaaaaat aagattgcgt
    13021 tttatatcgt aatataaggc gtttacggtg aaatcacggc gctctgcatc ttcacggagc
    13081 gtgccgtaaa cattatcacg cagtagcatt ccttcatcgc tgactttaga aattttgtca
    13141 ttgcgatgag attcgtgtcc tgcacggaaa gtcgccactt caaaaatttc acgcccgtaa
    13201 acaatgtgtg ccaaacggaa acgacgtccg attaatcggc attgcctacc aaaaatacgc
    13261 tgaatttctt caggtctcgc attagtcgca atatcgaaat ctttaggctt tttctccaag
    13321 agtagatctc gaatacagcc acccacaata taagcttcat aaccggcaat ctgcaattta
    13381 tcgacaatcg aaatcgcatt tttaggaaaa tccttcgggc taattctata ttggctggca
    13441 ttcaccgcaa atttcttgtg taacaaatgg gagggaggat tatcttgttg tttcttccca
    13501 cctctataag tcttttggtc cgttaagttc ggggctgggc gtttagaacg ggaagttttg
    13561 ggttctcttt ccgctctttt ttcactctct accttttcat ttttctgagc cgacttttta
    13621 ggcttggaaa ataatgattt aatataatta aaaataatac acctctaaat agtaaatgcc
    13681 tcttaaaaaa gaggcaccct gtaaagttga ttataagcct agctgctttt ctcgaatttc
    13741 agctaaagtt ttacagtcaa tacataaatc agctgttggg cgtgcttcta aacgtttaat
    13801 cccaatttct acgccacagg tttcgcaata accatattca tcttcttgga ttttatacaa
    13861 ggtttgctca atttttttca ataatttgcg ttcacgatca cggttacgaa gctctaaact
    13921 gaactcttct tcttgactgg ctcggtcgtt cggatcggca aagttagtca tatcttcttg
    13981 aagctggctt ttggtacgct ccgcttcttc cataatttga ctgtgccacg ctttcagaat
    14041 tttggtgaaa tgctcacgtt gagcttcatt catatattct tcgtcttttt taagctcata
    14101 aggctcaacg cctgctaatg ctaataaacc gagtgaggtt ttagccgctt gagtcataga
    14161 tatttactcc ttgttccctg tttattggaa gttttattgt taaaattatt gcccgaatac
    14221 agactaactt ccttgtaacc gcactatacg aaaaaatcaa catcttcggg ttttaaataa
    14281 tggcggctat aaataacaca aaaacgactc attaatcaaa tgaatttgca aaaattttgc
    14341 attcaaattg atttgattgc acaaaaaaat agccgaacat ccattcggct atttttcaaa
    14401 cactttttac cagctctttg accaatcggc taaccatttt tggatagttt cagtagtcgg
    14461 tggcgtaaat tcaatggttt tataggttgg cattgccgca aaagccggat caacgccgtc
    14521 ggttaaaacc ggattcatta cgttgtggta agaaataata tgttgcgatt ccggtttgtg
    14581 caaaaattca agaaattgat ttgccaatgc tttttgttgg ctgcttttga caatagccgc
    14641 attttctact tgcaccaaat gcccttcctc aaacggagca gccacatatt tatcggtgtt
    14701 ttcgtgccac ttatgatata aaggcgatgt agtatagctt agtaccaaat ccgactcgcc
    14761 ttttaagaac gctccataag tttcagacca acctttgcct accgttaccg tatgttttgc
    14821 caatgttttc caagcagttg gagcttcatc gccatacact tgatttaccc acattaagaa
    14881 gccccgccca acgctgctgg tacgtgggtc ttggtaaatc acttttaaat cttggcgttc
    14941 caccaactct tttaaacttt ttggtggatt tttcagcttc tctttatcgt aaataaaagc
    15001 ataagaaccc gtgtcataag ggaagaaaat tttctgttgg gtttctaatt tggtctttgc
    15061 ttcaaactcg gcaaacaagc cacttttttc ggcttcaggt tgcaggaaat tatcaatacc
    15121 gaccaccaca tcagctttgg tttttgctcc ctccaaacgc aagcggttga acatcgttac
    15181 gctatcgtca agcggaacaa atttgacatc acagccacac tctttctcaa aattctgctc
    15241 taatttagga gcaggtccat attccgaagc aaacgagctg taagtgtaaa cggttaaggt
    15301 cggttgattg gcataaacac ttgcacttaa taacgcaagt gaagagagga taaatttcat
    15361 aacaattcct tctaaattaa atgaatagaa ggatttaaga aaggcaagtt gctatctcaa
    15421 atccctacgc tgatattaat cagatcaggt tctatgggta tttctcagcc acaagcggtt
    15481 aaatttcggc aaaaatttgc aaatttttac gcaaaaacaa ccgcttgttg caccccaatg
    15541 agaacggctt cattgtaaaa agtttgcagg cagatttcaa ccaaagcaaa cgtttgctat
    15601 ttgtattaaa aatccgtata ctaccgccct agtccaaaca aggaactaga aatgaattgc
    15661 acaacatcgg ttaaatcaaa ttcccaacag ctacgcttca actttagagg cgtagctgtt
    15721 tttctattca tttcaaacca ttcaaaatcg ctgttatagc gatttttttt cgcacgcttg
    15781 caattaccgc aagcggttga gccacattaa cagagagaaa aggaataaaa atgagccaat
    15841 tgattcgtac cctaaaagga catattcgtg atgaaatcat caaaaaaggc ggttgggtaa
    15901 atgcacacgc ccacgcagat cgtgcattta cgatgacgcc tgaaaaaatc accgtttacc
    15961 aaaatgcgaa tctacagcaa aaatgggatt tggtcgatga ggtaaaacgt aattcaagcg
    16021 tagaagatta ctaccgccgc ttttcccaag cgattgaatt gatgatttca caaggggtaa
    16081 cggcatttgg tacttttgtg gatatcgacc aagtatgtga agacagagcc attattgccg
    16141 ctcacaaagc ccgtgaagta tataaaaacg acatcatctt gaaatttgct aaccaaacgt
    16201 tgaaaggcgt gattgaaccc aatgcgaaaa agtggttcga tatcggctcg gaaatggtgg
    16261 atatgattgg tggcttgcct taccgtgacg aactggatta cggcaaaggc ttggaagcaa
    16321 tggatatttt aatggataaa gccaaatcgc tcggcaaaat gctccacgtc cacgttgatc
    16381 aatttaactc acctaaagaa aaagagaccg aacaactgtg tgataaatcg attgagcatg
    16441 gtatgcaagg gcgagtggtt gccattcacg ggatttccat cggctcgcac ccgaaagaat
    16501 atcgaaaaat gctttaccaa aaaatgcgtg atagccaaat gatggtcatt gcctgtccga
    16561 tggcgtggat tgatagcgga cgtaaggaag atttacaacc gttccataac gctttaaccc
    16621 ctgctgatga actcattcct gaaggcatta ctgttgctat cggcacagat aatatctgtg
    16681 attatatggt accgctttgc gagggcgata tgtggcaaga actcagcctt ttatcagcag
    16741 gctgtcgttt tactaaccta gaagaaatgg caaatattgc cagtatcaat ggtcgaaaag
    16801 tactgggttt agtctaactg aataaacgaa aggcgaaccc taggttcgcc tttaataaca
    16861 gcataaccga ttatttaaac aagttaccca aaatcccttt tgccgcttga gctaataagt
    16921 catttgcatc aaagtttttt aagtcttcaa cattaccatt cggggttaaa gtatccacga
    16981 tttttggcaa gaactgggct aataaatcgc tcgcatcagc agcatcaaca cccgcttctt
    17041 gagccacatt ttgaatgttt tcttgcccga acacattact gatttgttcg cccgaaacag
    17101 gtgcattgtc ttcatcgctg ctgatccaag tgcgtaacac atcatctaat ccgccttgtt
    17161 ggaatttggc aatcagccct tcaatcccac cttgtgaggc gagtaaattt tgaattaatt
    17221 gagttgcagt tgattggtta ttgccactca atactgaaga agccaatgaa cctaaaatat
    17281 ttcctaacat aatttctcct atgcaagcgg tacaattttg cgaaaatttt gcaaaaaaac
    17341 agcaaacttc gaccgcttgt aatcacttta acgagctaaa atggctcgca ctctttccaa
    17401 atcttccggc gtatccaccc ccacttgcgg ggcttctttc gccggttcga tatgaatttt
    17461 ttcaccgtgc cataaggctc ttagttgctc aagggactca agctgttcta attgtgtcgg
    17521 ctgccacgcc acatattgtt tcacaaaacc ggctcggtaa gcgtaaatgc cgatatggcg
    17581 taaataacct tgagcggcaa caaaactgtc atcacaatcc ggaaaactgt ctctggcaaa
    17641 cggaatgctg gctcgggaaa aataaagtgc cattccgttt ttgtctgcca ccactttcac
    17701 cgcatttggg ttaaatagct cttctcgggt cgctaatttc acaccgagtg ttgccatatt
    17761 gacttgataa gcatctaaat tttgagctac ctgagtaaca attaccggtg gaatcagcgg
    17821 ttcatcaccc tgaatattca caataatttc gctgtcggca atctgcattt tttcgatcac
    17881 ttccgccaaa cgctcagtgc cggaattgtg tttttccgaa gtcatacaga cttccgcccc
    17941 aaaagattga gcgagggctt caatttcagg gtgatcggtg gcaataatca cacggcttgc
    18001 accggcaagc tgagcttttt cccaaacgtg ttgaatcatc ggtttgcctg caatatccaa
    18061 caacggctta cgaggcaaac ggctggaagc gtaacgagca ggaataatta cagtgaattt
    18121 cataggctat ccttatgctt cttgagcatc taagaaacgt tctgcatcca gggctgccat
    18181 acaacctgtg ccggcagagg taatcgcttg gcgatagtta tgatccatca catcaccggc
    18241 agcaaatacg ccttccacag aagtggcagt agcattgcct tccaaacctg atttcacttt
    18301 aatgtagccg ttttcaagtt ctaattgacc ttcaaacatt gccgtattcg gtgcgtgccc
    18361 aatcgccaca aagaaaccat caaccttaat ttcttcgctc aattcttctt tggttgattt
    18421 taaacgtaag ccggtaacgc ccatattatc gcctaacacc tcatcaaccg tacggtctgt
    18481 gtgcagaata attttgcctt cttcaatacg tttttgcaga cgaccaagca agattttttc
    18541 agcacggaag ctatcacggc ggtgaattaa atgcacttca cttgccaaat ttgccaaata
    18601 aagtgcttct tccacagcag tattcccacc accgactacc gcaaccggtt tattgcgata
    18661 gaagaaacca tcacaagttg cacaagccga cacgcctttg cctttgaaat gctcttcaga
    18721 aggcaaacct aaatatttcg ccgacgcacc ggtggcaata attaacgcat cacaggtgaa
    18781 agtgtataaa tctccgttta gcgtgaatgg gcgtttagat aaatccactg aatgaatatg
    18841 atcaaacacg atttcagttt caaatttttc agcgtgcttg agcattttgt tcattaactc
    18901 tgtgccggta atttgttcaa attcccccgg ccagttctcg atttcatcgg ttgtagtgag
    18961 ctgtccgcct tgttgcatac cggtgactaa aaccgggttt agattggcac gggcggcata
    19021 aacagcagcg gtataaccgg caggccctga gcctaaaatt aaaagtttgg tgtgtttagt
    19081 ggtcataatg aatcctctga ataaatatta atgtttattt tagcctaaat ttcagttcaa
    19141 ctctattttc gttttcgctc aacttttttc aattttgtga caaagttaac agaaatccga
    19201 ctgtgtttct tgtaaattgt gacagtctaa ttcatttaaa aggggcaaaa agatgaaaaa
    19261 attaattaat tcaatagaaa ccgtcttaga tgagcaactt tctggttttg tgaaagcaca
    19321 ttctgattta acacttaaca cagaacctgt ctatatttac cgcacaacgc caagtaatca
    19381 acctaaagta gcccttattt ccggtggtgg gagtgggcac gagccaatgc acgccggttt
    19441 tatcggtaaa ggaatgttag acggagcttg ccccggagca atctttacat ctccaacgcc
    19501 ggatcaaatg tttgaatgcg gtttagcggt tgatcgtggc gagggtgtat tgctaattat
    19561 taaaaactat accggtgatg tgctgaattt tgaaacagcc accgagctac ttgcagatag
    19621 cggtgtaaaa gtcgcaaccg tgttggttga tgatgatgtg gcagtcaaag acagtttata
    19681 tactgccggc cgccgcggtg ttgcaaatac agtattaatt gaaaagctgt taggagctgc
    19741 agcggataaa ggctataact tatccgaatt agccaaatta gggcatcagt taaacaataa
    19801 cgggcattct atcggaattg ccttatctgc ttgtactgtg ccggctgccg gtaagccatc
    19861 atttacttta gctgaaaatg agatggaatt tggggtaggt attcacggag aaccgggcat
    19921 tgaacgtcgc ccctttgaaa atctggatag taccgtgaaa caaatgtttg agaccttaat
    19981 tgctcacggc gattatcaac gtaaaattcg ccattggaat acacaaaccc aacaatggga
    20041 cgaaatcgaa gaaagcaaag tggcattgaa aaaaggtgat cgtgttattg ctttagttaa
    20101 taacttagga gcagtgccac tatccgaact ttatgctgtt tatgatgcgt tagaaaaaga
    20161 gtgccaaact ttcggcttaa gcattgaacg cagtcttgtt ggatcatatt gcacctcgct
    20221 ggacatgcaa ggtatctcta tcacattact gaaagttgat gaggatattc tcaaactatg
    20281 ggatgctccg gtaaatacac cggcaatgcg ttggggtgaa taattagatt gaaaggataa
    20341 gttatgacaa tatcaaaaca acagatctta aaatggcttg aaaattgcca acaggtaatg
    20401 actgaaaaac gggattattt aaccgaattg gataccgtta ttggcgatgg cgatcacggt
    20461 ttaaatatgc agcgtggatt tagtaaagca cttgaaaaag cccataccgt gcaagataaa
    20521 gatatcggca ccctgcttaa aaccgttgga atgacattat tatcgcaagt agggggagcg
    20581 agcggccctc tctatggttc attttttatt aaagcggctc aaggtgctac aggcaaagag
    20641 caactgagct ttaacgagtt actggcactt ctacagcaag gagcagaagc tatcgtacaa
    20701 cgtggacacg cacaagttgg cgataaaacg atgtgtgatt tatggctgcc attactcgaa
    20761 gacttagctc aagccgatag cactcaacca attgctcaaa tcttattaca agcggtcgaa
    20821 aaagcagaaa attttgcaaa tcaaaccgct ccattaatgg cgaaaaaagg gcgtgccagt
    20881 tacttgggcg aacgcagtat cgggcataaa gatccgggag caacgtccag ctactatatt
    20941 tttaaagcct tagctgaagc attacagtag gaggggtaaa tggttaattt agtgattgtt
    21001 tcacatagtg ctaaactggc ggaaggcgtg ggggaaattt tggctcaaat ggcacagggg
    21061 ggctgtaaaa tcgcccttgc cggcggcatt gaggatacct caaatcctat cggcactgat
    21121 gcggtaaaaa taatgcaagc gattgaatcg gtatttagcc cagacggtgt gattgtgttt
    21181 gtcgatttag gcagtgcaat tttaagtacc caaaccgcca tagatttact ggagccggaa
    21241 cttgccgaaa aagtgatcat tagctacgct cctttagttg aaggtgcttt tgctgctgta
    21301 gttgcagcct ctgccggcga tagtttagaa gcggtattaa atgaagcgaa cgaagccgct
    21361 gctcttaagc aggaacaagc caattagtaa aaatgccgta tcgaaaaata cggcatttta
    21421 gttttattag tacaatagtg aataaagctg tcttctaaat ttcggcacaa tcgggtctgt
    21481 acccagtgca gataagattg ccagaaattg gctttttacc tcgccgttcg ctacatttaa
    21541 atccgatttt aaccaatcaa aaagtaacgt gagtgcttct tcatttttat gagcttggtg
    21601 tagctgattt gccaatttaa tcgcaatttc aggggttttg ctgttcgcat aatccgcttg
    21661 aagctgttgt aattcgggcg attcgctggc ttgttccaat aattcgattt gggcttgcaa
    21721 accgtgccaa cggctgtctc tgtcttgaat cgggatcgct ttaaggattt cacgagcctc
    21781 ttccacgctt ttcattgcaa tgtaagtttc ggcatagagc aaagcgaaat cgctgttttt
    21841 cttatcggtc atttcccacg ctgatttcag cagcggcaag gcttcggtat aacgctgatc
    21901 ttgcaataat tccaaggctt gttgaaattt cagctcttcc tctttcggta aaatatttgc
    21961 caaccgaatc cgtaattcct gctcacttac tgcaccttga attgcatcaa tcggctgacc
    22021 gccggcaaac aaatagagcg ttggaatttg ttgaatgcga aattgagcag ccaatgcttg
    22081 ttgctcatca caatttaccg tcgccaataa aaattggtta gggttttcat ccgaaatacg
    22141 acgtagtaag gcatccattt ccaatgaagc cacttccctt ggcgaaataa aatgaaacac
    22201 cgtcggcact tgtgccgctg ctgcccatac ttcactaaaa ttctgctcgt ttacatttac
    22261 aaagtagtcc atacaaaacc ttcttaagct aaaaattact ttttatattg tagcattaat
    22321 aagaagtttc aataggtagc ccgtttctct cccacgcttc aaaaccacct tttacactat
    22381 aaacattctc aaacccttgt tcgattaaaa actgtgctgt actttggcta ctcactccgt
    22441 gatagcaaat cacaaccacc ggttcgtcat aatcgacctc gtctaaaaac ttaccataac
    22501 tctggttggt taaatgaaac gcatcctgtg ggtggctgta aacataacgg cgggcatcac
    22561 gcacatcggc aagcaccgca ccttcgtttt cgattaattc ccaagcctgt tgcgggctaa
    22621 tttctttaaa tgtagtttcc ataatcactt tccctcaaaa aacgtgcaac tataccataa
    22681 aattctaaaa agaatggcta ttattccaag ttgaaaattt gatgtttgtc agatataatt
    22741 gcaaactatt ttcaaatgca ctgatttaaa actaccacag tttgaggatt ttatggagca
    22801 attatttcaa tttttagaac atcatactga ctttgttata ttaggattac taggcttatt
    22861 aagcgtaatc tgcctttgga aagctattga acgtattatg ttttattcca aagtagatat
    22921 ttcaaaatac gataccctcg aagaattaga catcgattta acccacaatt taactaccat
    22981 ttcgactatc ggctctaatg ccccttatat cggcttatta ggaaccgttg tcggtattct
    23041 tttaactttc tatcatttag gtcaatcagg tggtgatatt gatgcggcct ctattatggt
    23101 aaacctttct ttagctctaa aagccactgc tgtcggtatc ttagtggcaa ttattgcaat
    23161 ggttttctac aatggttttg gtcgtaaagt ggaagaaaac aaactgaaat ggttggcatt
    23221 acaatcaaaa aagcctcatc aagcggtgtg atttagtcca ttttttgcaa attaataagg
    23281 cagaacaatg aaaaaattcg atgaaatcaa catcattcca tttattgata ttatgttggt
    23341 attactggct atcgtgttag ttaccgcctc tttcatttct cagggtaaaa tccaagtaaa
    23401 tgtaccgaaa gcaagtacaa cacagccaat gaaagcagac gatttagcga agcttttaac
    23461 cattaccgaa aataatgagt ttttctttaa tgatcaagca ataactaaag agcaattaat
    23521 tgctgaagtt gccacttggg ataaaagcca aaaagtgtca ctcaaagttg atggtgcagt
    23581 cgccttcgaa aaatttgtgg aattaaccga tatcctttct gcaaatgaaa ttaaaaatgt
    23641 agcaatcatt actaaaaaag aaactgctcc agcaccttca agcacacctg gtagccctgc
    23701 acaagtgcca gcagtcgcac cttaaatagg gtaaacaggt atgaaaaaaa aacattctcg
    23761 tatcggctta ttggcatctt tggctattca cactgcctta attggtggca cttttgcttg
    23821 gttacataac cacaaagtgg aaaaaattgc cgagcaaaac tctctttcta tggaaatggt
    23881 ggcagcatta ttagagcaac ctcaggttgc cgttgctccg gatcctatta ccgaagaggt
    23941 caaagaaatc gaaccggaga aggcagaagt accacctgaa cctaagctta ctccggagcc
    24001 aactccagag ccggaggcaa ttccagatcc tacgctaaat ccgaaaaagg aaaaaccgaa
    24061 agaagaaaag cgtaaagagc gacaccacca taaacataag catcgtcaca aagagaaaag
    24121 ggaagagcta caagagcaag aaaaaccgaa agataagcct aaagagcgcc cgaaacatca
    24181 tcacaagcat attaaagcat tagagcgcgg gccagagata aaacaaggta ttgtcgctaa
    24241 agcgattcca aatgcggctg aaggtgaaaa agtggttgcg ggggttgttg gagggcaaaa
    24301 aaacggttca ccaaattcaa cctcaactac aggttcagca aatactgggg ctagcagtgg
    24361 taatgccagt gaacttgctg cttataaagt cgcattacag cgagcattac agcgtaaggc
    24421 gaataactct taccctcagc gtgaaaaaat gatgcggaaa accggtacag ttacgattaa
    24481 atttaccatt acatcgtccg gttcaattac aaatgtatcc gtactcaatt catcgggaaa
    24541 tagtaattta gataacgctg cggtacgcag tgcagaaggt ttgagtatgc ctccgccacc
    24601 ggcaggcttc cctcctacgg taacagtacc ggtaagattt gcccttgaat aaaaacagcc
    24661 ctcaaaagca atgtactttt gagggctttt tattgcaaaa aattgactaa aaatgaccgc
    24721 ttactgcttg cgaatatcgc catttctcac attcggactc acttcacccg tacgagtttc
    24781 tgtgcttaac gaggtagaat ttactttcgt aaaagtataa tccgctttgc tgaattcacc
    24841 tgtttgatta tttaagaaga aattgcggta ctcttttacc cataatgccg ttgcgccaca
    24901 aactgccaca ggcataacta ctaaattcac aaatggaatc atcgtgaata ggctcactaa
    24961 agcaccaaag gtaaaattta aagtgcgaga ctgcgacaac gcatttcgca tacgctgaaa
    25021 actgatttta tggttatcaa acggatagtc acaatattga atggcgatca accaagcacc
    25081 gaagataaaa gtcagcacag gtgctacggt ttgcccaacc acaggcaaaa agcctaatac
    25141 aaaaagagca atcagccttg gaatgctata catcattttt tgccattctc gtttcagcat
    25201 tcgaggcaca tcttttaaca ttgcaccgaa tgacatatcg cccaaaggct cgccggtaag
    25261 ttgcagttcg actttttctg ctaataacgc attaaaggga gcagcaacaa agttagccaa
    25321 ggtagtaaaa gcaaaataaa atcccactaa tatcatcaaa aaaatcagtg gaatcagtac
    25381 aaagctaagc cattccagcc agcttggcag taacatatcc aacaagccac cgatattggc
    25441 aaaaaatccc caaatcagcc ctaccatcag cacggtatta ataagaattg ggaaaatcac
    25501 aaaaggcaaa aattgacgtt gtaacaataa ccgccagcca tatataaaat aatgaaagcc
    25561 taatccgact tgattttgcg gctcagaaaa aactgaagac ataatgattc cttattttct
    25621 atcaaaatta attagaaata tggtaagcta cgcacattat tttcgcaaat atgaaaatac
    25681 attttttatt gattatttga taaagaggac acaatggaac tgcatattat tttttttatt
    25741 cttgccgggc tactgattgc tgtactaatt ggatttagcc tttggtccgc tcgtcgtgaa
    25801 aaatcaaata ttttttcaaa tacattctcg accagaccta ttgctgtgcc accacagaat
    25861 aataaaatga gcaatagcat cccaacctca ttacagccac aaaatgagga ttatcaagca
    25921 atgtctgtgc cggaagaaaa tccgcaacag atacaacagg aagttgaaag ctcgctgcaa
    25981 aatatcaaaa tcagtttgcc gggcggaaat cctcagcaac agcctgagca aattcagccg
    26041 atacaatcgg tagcagaacc aacggttagc acttacggtc agaaaatttc taatcagcaa
    26101 catatccaat acaatacgcc ggattttgca aacccaatgg tgaacacagt ggtggaagaa
    26161 acacctcaag cagtagctat cgaacctcaa gaaacccaat cggcagagga agaaaatcca
    26221 tttgtaaccc tctatcttta cgcaccacaa aatgaagctt tccgcggtga ttttgtagta
    26281 cattatttag aagaagttgg cttacgtttt ggcgaacacc aaattttcca ccgccatcaa
    26341 catattgatg attcattaag tccgattatt ttcagtgcag caaatttaat gaacccagga
    26401 acctttgatt taaataatat ctcaaacttt gcgacagctg gtttagtgct atttatgcaa
    26461 cttccaaccg aaggcaatga tgtggctaat atgaagttgt taatcagtaa cgctgaacac
    26521 ttagcagcat cgttaggggg attcttatat gacgataatc aacagccttt tggcgaagaa
    26581 tcccgccaac gttatatgca acgtgtgatg cactaattgc gaaaaaatta ataaatccga
    26641 ccgcttgtac aagcggtctt ttttgtagag aattttacca atgtctttat ttaaccaaac
    26701 ctctttattt gaagagaatg ccacgcccga gttagtcgag aaaatcgaaa ctattcgcca
    26761 aacattgcgt caatacgagt atgaatatca cgttttagat aacccaagcg tacccgatgc
    26821 cgaatacgac cgtttaatga acgagctgaa aaatctggaa tggcagcacc cggaactgat
    26881 tagtgccgac tcgccgactc aacgggttgg tgccaaaccg ctggcaggct ttgctcaagt
    26941 cacccacgaa atccctatgc tttcgttgga taatgccttc tccgatgaag acttagatgg
    27001 ctttttacgc cgtatggaaa gctatatcac cgcctctcct tcgcaactgg aattttgttg
    27061 cgaacctaaa ttagacggct tggcagtgag tattttatac gaaaacggta tacttacccg
    27121 agctgcaacc cgtggcgatg gcacaaccgg cgaagaaatt accgctaata tccgcaccat
    27181 tcgcaatatt ccgttaaagc tcaacacgac taatccacct gctcgattag aggtgcgagg
    27241 cgaagtcttt atgccacaat caggctttaa tgagctgaat gagaaagcct tagctaaagg
    27301 cgaaaaaacc tttgccaacc ctcgcaatgc cgcagcaggt tcgcttcgcc agttagatcc
    27361 gaaaatcacc cgccagcgtc cgttagtgct gaatgcctac ggaattgggg tgtatgaaag
    27421 cgatgacgaa ctgccagcca cccatttcga acgtttgcaa tggctgaaat cgcttggtat
    27481 tcctgtaaat aatgaaatcc gcttggttca aggcaaagct gctctcttgg cttattatgc
    27541 ggagattcag gctaaacgcc caacattagg ctatgatatt gatggcacag tattgaaagt
    27601 aaatgacatt gccctacaag agcaactcgg gtttatctct cgctcctctc gttgggcgat
    27661 tgcgtataaa tttccggctc aagaagaaat gacaatcctc aaagaagtgg acttccaagt
    27721 gggaagaaca ggggcaatca cccctgtggc gaaattagag cctgtttttg tcgctggcgt
    27781 aacggtaagt aacgcaacgc tacacaacgg tgatgaaatc gagcgacttg gtattgtgat
    27841 tggcgatacg gtaattattc gccgagcggg cgatgtaatt ccgcaaatta caggcgtgat
    27901 gttagaacgc cgcccggaaa atgcaaaaaa aattgaattt ccgaccgctt gtcctatctg
    27961 tcactccgct gttactcggg tagaaggcga ggcagtagca cgctgtaccg gtggtttatt
    28021 ctgcgaagct caacgtaaag aagccttgaa acatttcgtt tcacgcaagg caatggatat
    28081 tgacggcgta ggcgaaaaac taatcgagca gttaatggct cgagagctga ttgaaacgcc
    28141 tgccgatctg tttaagctcg atcacactac cctaatgcga ttagagcgaa tgggggaaaa
    28201 atcggctcaa aatgctctca acagcatcga aaaagcaaaa aacactactc ttcctcgctt
    28261 tttattctcg ttaggtattc gtgaagtggg cgaggcaacc gctctcaact tagccaacca
    28321 ttttggtagc ttagaggcga tccgccaagc cacttttgag caattaaaag aagtgcaaga
    28381 tgtgggcgag gttgttgcca accgtattgt cagcttctgg caagaaccgc ataatgtgga
    28441 agtggtggaa gatttgatcg ctcaaggtgt acgttgggaa aacgttgtga aagccgaaat
    28501 tgtagataac ccgttaaaag ataaaaacgt ggtcttaacc ggcacgctaa cccagctcac
    28561 ccgtgatgaa gccaaagccc tattacaaag tttgggctgt aaagtctccg gctcggtttc
    28621 cagcaaaacc gattacttaa tcgccggcga aaaagccggt tccaagctgg caaaagctca
    28681 agagttggga attcagattt taagcgagca acaatttatt gagcttactc aacattaaca
    28741 aacaaagggg cagatgattg aaatctgccc cttattctat ctattgaatg cttaaaagca
    28801 ttaaccaatc aatttaaact gctgagctaa aaacagtaac gtaaagctag ttaagtaaat
    28861 taagccgata actgccaacc aaaataacca gccttttgca cgatgctcac cttttagcac
    28921 taaagagaaa tttaaccacg caaaaacagg ggtagaaaca aatgatgaaa tcattgcaaa
    28981 ttgtaacatt ttcgccacat cactcataaa ttggctaata attaaaatgc cgatgagtgc
    29041 agttaccgtc atccaaatac ttaattctgt ttgtgaatgt tgcttacgcc cgctaattaa
    29101 acgcaacgct tctgcattcg ctcgtgaata tccatcaata acagtgatcg ttgtgccgaa
    29161 catacacata aatgcaataa atgcgattaa aagcttagac cagtcaccaa tcgcaaaagc
    29221 atacattttg attaattgag caatataggc aacaccggct tgttctactt tctcacctga
    29281 accgtactga actaacgcac ctaatgctaa aaaaacaatc gccaaaattg ccgtgccgat
    29341 ataacccaca ttaaaatcaa aaataccgtc ctcataattc actttactta aacgtttttt
    29401 cgcaataacc cacattgagt taatggcaga aatttcaatt ggtgcaggca tccagcccat
    29461 taatgcaacg atgaacacaa gtgctgctaa attccacggc gaaggctcta cgaaatctgg
    29521 agcaatttca acgccacctt ttaaagcagc aatgatcacg gcagataccg tagagatggt
    29581 taaagcaatc ataatccatt tagataatgt atctaagaat ttatattgcc ctagaagtaa
    29641 aataccccaa gtgaccacaa tcacaatagt tcctgccatt ggaatgctta aatctaacgg
    29701 aatacctaaa gcagaaaaaa taaattttag aatcgctgct gaaacaatcg ccaccgcagc
    29761 tgtgttaatc attgcagcaa aaacgttaag gataaagaaa ataataagat aaaactttcc
    29821 tttttctttg tagccttcca ataaagtttt accgctagct aaggtatatt gcacaccaaa
    29881 gcggaaaaac ggatatttaa ataaattggc taacagaata ataatcgcca gctgccagcc
    29941 ataaatcgca ccagattgtg tagaggcaat aatatgcgaa ccacccactg ccgcagatgc
    30001 catcaaaatc cccggcccca ttgcagcaaa tttactttgc caagttgaat gttctatttg
    30061 ttgtgtcata tagc
//
LOCUS       DANLXQ010000012        28656 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.012, whole genome shotgun sequence.
ACCESSION   DANLXQ010000012 DANLXQ010000000
VERSION     DANLXQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28656
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.012"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            25..999
                     /gene="znuA"
                     /locus_tag="PXD94_000420"
     CDS             25..999
                     /gene="znuA"
                     /locus_tag="PXD94_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL6285322.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPREKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(1051..1371)
                     /locus_tag="PXD94_000421"
     CDS             complement(1051..1371)
                     /locus_tag="PXD94_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL6285323.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            1512..3875
                     /gene="rnr"
                     /locus_tag="PXD94_000422"
     CDS             1512..3875
                     /gene="rnr"
                     /locus_tag="PXD94_000422"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL6285324.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            3879..4145
                     /locus_tag="PXD94_000423"
     CDS             3879..4145
                     /locus_tag="PXD94_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285325.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEFLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
     gene            4368..4826
                     /gene="mraZ"
                     /locus_tag="PXD94_000424"
     CDS             4368..4826
                     /gene="mraZ"
                     /locus_tag="PXD94_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL6285326.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            4842..5780
                     /gene="rsmH"
                     /locus_tag="PXD94_000425"
     CDS             4842..5780
                     /gene="rsmH"
                     /locus_tag="PXD94_000425"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL6285327.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINGEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIGKAIMPSEAEIEANARSRSAVLRIAEKR"
     gene            5799..6110
                     /gene="ftsL"
                     /locus_tag="PXD94_000426"
     CDS             5799..6110
                     /gene="ftsL"
                     /locus_tag="PXD94_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL6285328.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            6114..8174
                     /locus_tag="PXD94_000427"
     CDS             6114..8174
                     /locus_tag="PXD94_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL6285329.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRNGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKKNFSKEKVDFDPRS
                     ILNTKSEGYWTQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRERQKFQALSKEI
                     GRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVESRRFYPLAEES
                     SQLIGFTDKDDMHGSEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIRSEKQYDPQDV
                     MLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPSFNPNKRDSFK
                     PELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNGHTIKDVAPRD
                     SLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQRGTNGDRKRW
                     ADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIGERVLPEKTTR
                     DVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTIHKLEKGKYVEKYIAYTAGLAPASDP
                     RFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENTQSAVREIKLE
                     RPIN"
     gene            8183..9673
                     /gene="murE"
                     /locus_tag="PXD94_000428"
     CDS             8183..9673
                     /gene="murE"
                     /locus_tag="PXD94_000428"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL6285330.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEIELDPKFAKFNLDRTACCKVVSVPRLPKLL
                     SEIAGALYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQHNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPKFAGN
                     HQFVKATAVKFTLQGASIAFESSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQE
                     LVKTAPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHEDYQIIGTEKLHFSDQETARKYLF"
     gene            9761..11155
                     /gene="murF"
                     /locus_tag="PXD94_000429"
     CDS             9761..11155
                     /gene="murF"
                     /locus_tag="PXD94_000429"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL6285331.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIGSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDTYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            11176..12258
                     /gene="mraY"
                     /locus_tag="PXD94_000430"
     CDS             11176..12258
                     /gene="mraY"
                     /locus_tag="PXD94_000430"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL6285332.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWS
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            12322..13623
                     /gene="murD"
                     /locus_tag="PXD94_000431"
     CDS             12322..13623
                     /gene="murD"
                     /locus_tag="PXD94_000431"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL6285333.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     ADKLDVTIPLHTGGLNSEWLLKSDLIVISPGLAVATPEIQQAIQAGVEIVGDVELFCR
                     EAKAPIIAITGSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLEL
                     SSFQLETTYSLKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQT
                     YPIQAVKKVIRFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAE
                     AANVPREGIVKALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGT
                     LYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAI
                     RPQLKKGDMVLLSPACASLDQFKNFEQRGEVFMALAQGQHA"
     gene            13623..14801
                     /gene="ftsW"
                     /locus_tag="PXD94_000432"
     CDS             13623..14801
                     /gene="ftsW"
                     /locus_tag="PXD94_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HDL6285334.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLLLILLTVRALKISKESLVLEERFKGYMAFGIAIWVFLQG
                     FVNLGVASGLLPTKGLTFPLVSYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI
                     "
     gene            14920..15975
                     /gene="murG"
                     /locus_tag="PXD94_000433"
     CDS             14920..15975
                     /gene="murG"
                     /locus_tag="PXD94_000433"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL6285335.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAKRGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMSMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            16051..17478
                     /gene="murC"
                     /locus_tag="PXD94_000434"
     CDS             16051..17478
                     /gene="murC"
                     /locus_tag="PXD94_000434"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL6285336.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSTGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
     gene            17568..18479
                     /locus_tag="PXD94_000435"
     CDS             17568..18479
                     /locus_tag="PXD94_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL6285337.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQAVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            18479..19270
                     /locus_tag="PXD94_000436"
     CDS             18479..19270
                     /locus_tag="PXD94_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL6285338.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFV
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIQEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVIIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            19293..20564
                     /gene="ftsA"
                     /locus_tag="PXD94_000437"
     CDS             19293..20564
                     /gene="ftsA"
                     /locus_tag="PXD94_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL6285339.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYHVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARTFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            20597..21844
                     /gene="ftsZ"
                     /locus_tag="PXD94_000438"
     CDS             20597..21844
                     /gene="ftsZ"
                     /locus_tag="PXD94_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL6285340.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            21876..22796
                     /gene="lpxC"
                     /locus_tag="PXD94_000439"
     CDS             21876..22796
                     /gene="lpxC"
                     /locus_tag="PXD94_000439"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL6285341.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYKSGHGVNNKLLRAV
                     LENKNAWEFVTFEDKAEVPQGYQVQQEIFI"
     gene            22969..24651
                     /gene="recN"
                     /locus_tag="PXD94_000440"
     CDS             22969..24651
                     /gene="recN"
                     /locus_tag="PXD94_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL6285342.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDATHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQPQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(24698..24773)
                     /locus_tag="PXD94_000441"
     tRNA            complement(24698..24773)
                     /locus_tag="PXD94_000441"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24738..24740),aa:Ala,seq:ggc)
     gene            complement(24853..26292)
                     /gene="gltX"
                     /locus_tag="PXD94_000442"
     CDS             complement(24853..26292)
                     /gene="gltX"
                     /locus_tag="PXD94_000442"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL6285343.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
     gene            26467..26542
                     /locus_tag="PXD94_000443"
     tRNA            26467..26542
                     /locus_tag="PXD94_000443"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26500..26502,aa:Val,seq:tac)
     gene            26591..26666
                     /locus_tag="PXD94_000444"
     tRNA            26591..26666
                     /locus_tag="PXD94_000444"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26624..26626,aa:Val,seq:tac)
     gene            26719..26794
                     /locus_tag="PXD94_000445"
     tRNA            26719..26794
                     /locus_tag="PXD94_000445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26752..26754,aa:Val,seq:tac)
     gene            26843..26918
                     /locus_tag="PXD94_000446"
     tRNA            26843..26918
                     /locus_tag="PXD94_000446"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26876..26878,aa:Val,seq:tac)
     gene            complement(27072..28565)
                     /gene="mltF"
                     /locus_tag="PXD94_000447"
     CDS             complement(27072..28565)
                     /gene="mltF"
                     /locus_tag="PXD94_000447"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL6285344.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYEKIEEKEIPTDKEITPASN"
BASE COUNT         8696 a         5509 c         6389 g         8062 t
ORIGIN      
        1 attaaatgcc ataaggggaa ttttatgttt aaaagaacat taatcggtct agccgtgtta
       61 ggtgcaagtg ttgctcaagc ggatgtatta accagtatta agcctttagg ctttattgcc
      121 agtgccatta ccgaaggggt aactgaaact aaagtattat taccggtaac tgcctctcca
      181 cacgattaca gcttaaaacc ttcagatgtg actcaattac aatctgctca attagtggtt
      241 tgggtgggtg acggcttgga aggcttttta gaaaaaagta ttgaaaaact gccgagagaa
      301 aaagtattaa cccttgaaga tgttgatgcg attgatgata ttgtcgataa agcaagcaaa
      361 aaagaagata aagacgatga tcacgatcat aaacatgaac ataagcacga acacaagcac
      421 gagcataaac acgaagggca taatcacgaa ggacataacc acgatcatca cggacatagc
      481 cacgatgaag attggcatat ttggctctca agcgaggcaa gtgagctggc agcagagcaa
      541 attgcggcac gtttaagcga acaaatgcct gaacacaaag cgaaaattgc ggaaaatctt
      601 gcaaaattta aagcaaatct aaccgctaaa aaagccgaaa ttgcgaaaca attagagccg
      661 gtaaaagcca aaggttacta cacattccac gatgcttacg gctattttga aagagaatat
      721 ggtttaaatt cattgggttc atttaccatt aacccaactg ttgccccggg tgcaaaaaca
      781 cttacagcaa tcaagaaaaa cattgcgaaa aacaaagcac aatgtttatt tgccgagccg
      841 caatttacgc cgaaagtaat tgaaacctta gcaaaagaaa ctaaagtaaa tgttggccaa
      901 ttagacccac tcggtgcaaa agttcagtta agtgcgactg cttaccctga atttttacaa
      961 gttttggctg acgaatttag ccaatgttta acaaaataag ttaaaaatcc cccatttaaa
     1021 tataaatggg ggattttgcg attacatcac ttaagctttt tttaagaatt ctgatttcaa
     1081 cataatttta ccgcaatcga cattatggtc accttgagct aaacggattc ctttgaattt
     1141 tgtacctttt ttcagtactt cagacgagcc ttttaatttt aaatctttga ttaataatac
     1201 atcgtccccg tcagataata aattgccatt actgtctttt acaattagca catcatcatc
     1261 ttgatccgtt tcttcgcccg tccattcata cgcacaatcc gggcaaacaa attgaattga
     1321 gtcgtggtaa gtattttcgc ttttacattc agggcaggtt gggtaaatca ttttaattcc
     1381 tcaaattaat aaagaaccag attatatacc gaaatccagc aaaacgctaa gcggtcatat
     1441 tttgccgatt ttttaccaat cgagctaaaa gtcagttaga atagccttaa ttacacaaaa
     1501 aggaggatac tatgatagtc gatccgaatt atcagcaaga attagaaaaa tacgaaaatc
     1561 cggtaccaag ccgagagttt attttaaata caattcgtga acataacgcc ccaatgagcc
     1621 gtgatgaatt attgtcggct tttcatattg acgatgaaga acgaatggaa ggcattcgcc
     1681 gccgtctgcg tgcaatggaa aatgacggtg aattggtgtt caccaaaggc aaacgctatg
     1741 cgttgccgga aaaaatggat ttaatcaaag gtacagttat cggccatcgt gacggttacg
     1801 gttttttgca agtggaagga cacgaaaaag gggcgactaa aagcgaagat tggtttattc
     1861 ccaacgccca aatggtaaag gtaatgcacg gtgactttgt gctggcacaa ccaaacggca
     1921 cagaccgcag aggtcggaaa gaagtccgca ttgtgcgagt gttggaggcc cgtaagaaac
     1981 agattgtcgg gcgttttttc cttgaaagcg gtatcggttt tgttgtgcct gatgacagcc
     2041 gcgttaacca agatattctc attccggatg aacaccgtat gggggcgaga atggggcaag
     2101 ttgtggtggt ggaattacag gaaagaaaag cgagttttag ccgcccggtg ggaatcatta
     2161 ctgaaattct aggcgataat ttagcgccgg gaatggaaat tgaaattgcc ctacgcaatc
     2221 acgatattcc gcacatttgg ccggaagggg tagaaaaaca aatccgccaa tttagtagcg
     2281 aagaagtgcc ggaagaagcc aagcaagggc gggtggattt gcgagatctg ccactgatta
     2341 ccattgacgg cgaagatgcc cgagatttcg atgatgctgt atttgccaaa aaagagggca
     2401 acggctggcg gttatgggtg gcgattgccg atgtaagtta ttatgttcgc ccaaaaaccg
     2461 cattagattt ggaagcccta aatcgtggta actcggtcta tttcccgaat cgggttgtgc
     2521 caatgttgcc ggaagtgctt tccaatggct tgtgttcact caatccacag gttgacaggc
     2581 tctgcttggt ggcggaaatg tcggtttcca gcaaagggga attacaagat tatcaattct
     2641 acgaagcggt aatgaactct cacgcccgtt taacttacac taaagtgtgg aaaatgttgg
     2701 aaggtgatga agccttgcgt gaacgctatg ctccattagt gccgcatatt gaagaactct
     2761 atgcaatgtt ccaaacctta atgaaagctc gccataaacg aggggcgatt gaatttgaaa
     2821 ccattgaaaa tcaatttatt ttcaatccgc aagggcgaat tgagcgaatt gagccgttaa
     2881 tccgcaacga tgctcataaa ttgattgaag aatgtatgat tttagccaat attgctgccg
     2941 ctcgttttgt ggaaaaagcg aacgaaccgg cgttatatcg gattcacgat aagccgagtg
     3001 aagaaaaatt gctcagtttt aaatcctttg tgcgtgagtg tggcttaacc tgggacgtag
     3061 gcttagatcc aaccccaaaa gattacggtg tactactgga gcaaattgcc gaacgtgccg
     3121 ataaagagtt gattcaaacg atgttgctcc gctcgctgaa acaagcagtg tatgcggcgg
     3181 ataatatcgg gcatttcggc ttggcattaa ccgaatatgc tcattttacc tcgccgattc
     3241 gccgttaccc ggatttactg ttacaccgtg caattaaata tctgatcgaa aaaggcaaag
     3301 gcaacactcg ctactatacc gatggcggtg gttatcacta caagttagat gatatggacg
     3361 aattcggcga taaatgttct gccaccgaac gccgagctga tgaggcaacc cgagaagtgg
     3421 cagattggct gaaatgcgaa tttatgcaag atcatcttgg cgaagagttt gatggcgtga
     3481 tttcgagtgt taccggcttt ggcttatttg taaaactcaa tgagctttta attgacggat
     3541 tggttcatat ttcaacgctg gataatgatt attaccacta tgatgcggac cgacaacgtt
     3601 taatcggtgg cagtgggatc atctatcgtt taggcgatgc agttagagta aaagtaatca
     3661 atgtgaattt agacgagaaa aaaattgatt ttgagttact caccggtaat aagaaaaccg
     3721 gcaaaaccgc caagaaaaaa gcaaaagcga cagaggttcc tgttttcaaa gagcctaaaa
     3781 cggtaaaaaa agccaaaaaa gtcgctgaga aaaaaccggc aaaatctgca aaaaaacgga
     3841 caaaaacgac cgctagtaaa tcaaggaaaa aatagcaaat ggattacaaa aaacaaattt
     3901 tattcagcct tttatttgct ttagtaattg caataataag ctattttttg tttacctata
     3961 tcccgtttga ttgtagcgat ctgagcaaac tcaacgattc acaacgttta gagtgccgaa
     4021 ctgccgaatt tctgggcgtt tattactggc tgccgcacac taccacgctg tttttcggct
     4081 cggtgttatt atattgggcg gtttatggca tgatttcatt agtgcaagca gtgctcaaac
     4141 gctaacaagc agttttgttt ttgcaaaaag ttgcaaatct catctgaata atccttccaa
     4201 gatcaaaggt ctatcaaaaa agatagacct ttattgtgta aataaactaa aactccttga
     4261 tagatgtatc acatatctat aaaattccag caattaagtg ggaaaaagtg gtaaattgtg
     4321 gaacttttcc ttaaatttaa cctcatttaa aaaggtaaat cagtaaaatg tttcgtggtg
     4381 caagttcaat cagcattgat agcaaagggc ggatcgctat tccgacccgt tatcgtgccg
     4441 agctattgga aaaacatcac gggatcttag tttgcactgt ggatatccgc cagccttgtt
     4501 tgttgcttta cccacttcac gaatgggaaa tggttgaaca gaaattgttg gcactatcta
     4561 attttgaccc tgttcaacgc cgtattcagc gagtaatgca aggctttgct accgaatgtg
     4621 aaatggattc ggcagggcga atactgctta gccctacact tcgccaacac gcacaattag
     4681 agcaacaaat tatgctggtt ggacaattaa acaaatttga aatttggcaa gataaacaat
     4741 ggcaagcaca aattgccgaa gatcttgctt ttggcagttc ggcagaaatt ttagactgcg
     4801 aagcattgaa aaacctgtct ctctagtaat tggaaattga gatgagcgag aacccaaaac
     4861 atattacagt attattgcac gaagcggttg acggcttggc aattaagcct aacggtatct
     4921 atattgacgg tactttcggg cgtggcgggc attctcgctt gattttatcc aaactgggcg
     4981 aaaatgggcg attaatcgca actgatcgtg acccacgagc gatagccgaa gctcaaacga
     5041 ttagtgaccc tcgttttcaa attgaacata cggcattttc tgccattccc gaactgtgtg
     5101 aacgaatggg gttggtgggc aaaattgacg gtattttatt ggatttaggt gtgtcttccc
     5161 cgcagcttga cgaagccgaa cgaggcttta gttttatgcg tgacgggccg cttgatatgc
     5221 gaatggatac caccaaaggc ttatcggcag ccgagtggct tgcccaagtt tcggcagaag
     5281 atttagcttg ggtattgaaa gaatttggcg aagagcgttt tgccaaacgg attgcacaag
     5341 cggtcgtttc ttacaataaa tctgcaaatg aaaaaatcag ccgcactttg cagcttgcgc
     5401 aaattattgc ggatgcggtg ccgtttaaag ataagcacaa acaccctgca acccgttcgt
     5461 tccaagcaat tcgcattttt atcaatggtg aattagacga gctggaaaaa gctttaaatt
     5521 ccgcattaac ggtgttagcc cctgaagggc gtttgtcgat tatcagcttt cattctctag
     5581 aagaccgaat ggtgaaacag tttgtgcgta agcatagtaa aggaatggac gtaccccgtg
     5641 gtttaccgat tttagaagca gagctaacca aaaatattcc gctgaaaacc attggcaaag
     5701 cgattatgcc gagcgaagcc gaaattgaag caaatgcccg ctcacgcagt gcggtattgc
     5761 ggattgccga aaaacgctaa cagtaaatag ggaagaatat gtcgaacgaa cgttatccgt
     5821 tacatcaaat catcattgat gatatcttta gccataacaa agtggctctt ttattgttga
     5881 ttggtgtagt gatttcagca gtggctacga tttggataac ccaccagact cgtttatcgg
     5941 tgagcgaaca agggcaactg attcaagcaa accaaaagtt ggaaagccaa tatactaatc
     6001 tgcaattaga agaaaacagc agaagcagtc gggcaagagt tgatgccgcc gctaaatcgt
     6061 tcggattaca gccgattaaa aaagagcaag aaattatttt agttgaataa aaaatggtta
     6121 aatcagttaa attaaaacgt aagcaagaag cccctgtcgc aaaagcagag cctcaacaag
     6181 cagaaggtaa aaaaacaccg agttttttac ctactcgctt tagagtggtt gtgtttttta
     6241 tgctattaat ggttggtggc ttactagcac aaaccgccta tattcagctt tttgactctg
     6301 aaagattaat taaagaggct aataaccgct cacttcgtac taaagaactc caatttaccc
     6361 gtggacgtat tttagatcgt aacgggcgtt ttctctctat tagtgtgcca atgtactcat
     6421 taacacttga cccgagagaa tactttgata ctaagctgcg tcgttctaac gaaagctggc
     6481 gtgccttagc gattgaaatg gaaacatcaa aaggtaaaat tgaggcaaac gttaataaat
     6541 ttattgaaaa gaaaaatttt tcaaaagaaa aagtagattt tgatccacgt tctattttaa
     6601 atactaaaag tgaaggttat tggactcagc ttgcccaagt tacaggtttg gattacaaca
     6661 gtttaataga aaaagtacgg aataacccta gctcggtttt tttacgttta gataacgaaa
     6721 atttagtaag agaacgccag aaattccaag cgttaagcaa agaaatcggc cgttcctata
     6781 atgacataat ggacgagctt tatagcaaaa accgccagcg attcctttac ttatcccgtc
     6841 atcaatctga agcgatcagc gaatatgcaa aagaactaga tattgatgca ttagtgatta
     6901 aagtcgaatc acgccgtttt tacccgctgg cagaagaatc ttcacagttg atcggtttta
     6961 ctgataaaga tgatatgcac ggttcggaag ggctggagcg tagttttgat tccttactta
     7021 tcggcaaaaa cggtaaacaa attatccgaa aagatgccaa aggcaatatt gtggaaaata
     7081 tccgttcgga gaaacagtac gatccgcaag atgttatgtt aagcattgat gaagaattgc
     7141 agtctatggt gtacggtgaa atcaaaaaag cggtacagga aaataatgcg gaatcaggca
     7201 ctgcggtttt ggttgatgtg caaaccggcg aaattttggc aatggcaaat gccccttcat
     7261 tcaacccaaa caaacgggat agttttaaac ctgaattaat gcgtaaccgt gctattaccg
     7321 acacttttga accgggttca acggtgaaac cttttaccgt attaaccgca ttacaaaatg
     7381 gggtaactta tcgtgatgaa gttattggca ctcgcccatt tgtcgtcaat ggacatacaa
     7441 ttaaagacgt tgctccaagg gacagcctta cgcttaccgg catcttgcaa aaatccagta
     7501 acatcggggt cagtaaatta gccctcagaa tgccggcaaa tgcgttagtg gatacttaca
     7561 cgaaagtcgg tttcggtaaa gacaccggtt taggcttggg tgaacaacga ggtacgaacg
     7621 gtgatcgcaa acgttgggcg gatattgaac gtgctaccct ttcctatgga tacggtttaa
     7681 atgtgacccc attacaactt gcccgtgctt atgcgacttt aggaagtttc gggctgtatc
     7741 gcccactttc gattaccaag gtcgatccgc cggtgattgg cgaacgggtg ctaccggaaa
     7801 aaaccacccg agatgtcgtg catatgatgg aaagcgttgc ggctaaaggc gaaggcggac
     7861 accgtgcagc ggtggacggc taccgtgtag ccatcaaaac cggtacaata cacaaattag
     7921 aaaaaggtaa atatgttgaa aaatatattg cttataccgc agggcttgcc ccggcaagcg
     7981 atcctcgctt tgcattagtg gtattaatca acgaacctaa agcaggcaac tattatggtg
     8041 gctttgtttc tgcaccactg ttctcacgca ttatgggtta caccttaaaa caacgtaata
     8101 ttaagccgga taacttaacg gaaaataccc aaagtgccgt gcgtgaaatt aaattagaac
     8161 gccctatcaa ttaaggttag ctatgaaacg tttactccct tttctgacag aacttgatgc
     8221 ttgggtggaa gaactcaccg agctaaaaca aatgacactt gatagtcgcc aagttcaagc
     8281 cggtgatctc tttgttgctt taaaaggaca tcaagtcgat ggcagaaaat ttattccgaa
     8341 tgcgatagaa caaggagcag caattatttt ggctgaggct gatgaagatc aaccggaaat
     8401 tgagcttgat ccgaaatttg caaaatttaa cctagatcgc accgcttgtt gcaaggttgt
     8461 ctctgttcca cgtctgccaa aattgctctc tgaaattgca ggagctcttt accataaccc
     8521 gtcacacaag ctgactttag ccggcattac cggcacgaac ggtaaaacga ctactgctca
     8581 attattagca caatggcata atttattggg tgggaaatcg gcagtaatgg gtactatcgg
     8641 caacggtttg tacggacaag tgcaagaagc ggtaaatacc accggctctg ccattgaaat
     8701 tcagcataat cttgcgaact ttgttgaact tggagcggat ttttgtgcga tggaagtcag
     8761 ctctcacggt ttggctcaat ttagagccga agccttggat tttgatttag cgatctttac
     8821 gaatttaagc cgagatcacc ttgattacca caacacgatg gaagagtatg cccaagccaa
     8881 attccgtctc ttcaacgaat taagcaccaa agctcaagta attaacgccg atgatgaaat
     8941 cgggcgtgaa tggctgacac aattaccaaa tgcagttgcg gtaagcaccg atccgaagtt
     9001 tgcaggcaac caccaatttg tcaaagccac tgcggtaaaa tttaccctac aaggggcaag
     9061 tattgcgttt gaatcaagtt ggggcaatgg cgagttacat agccgtttaa tcggggcttt
     9121 caatgtaaat aatctgctta ccgcctttgc cggactttta gctcttggct ttgatattca
     9181 agaactggtt aaaaccgccc cgaatttagt tggcgtagca ggtcgaatgg aatgcattac
     9241 cgctccaaat aagccaatgg taattgtgga ctatgctcac acccctgatg ctcttgaaaa
     9301 agccctacaa gcggctcgtt tacattgcga aggcgaactt tggtgcattt tcggctgtgg
     9361 tggagaccgt gacgccggca aacgcccatt aatggcgacg attgccgaga aattaaccga
     9421 taaagtgatt gctacagacg acaaccctcg caccgaagat aatcaaaaga ttatggcaga
     9481 tattgtgaaa ggttttagta aaccccaaca aattatccac aatcgtgaag aggcaattaa
     9541 aaccgccatt gagcaagcta aagccacaga tgtgattcta atcgcaggca aaggtcacga
     9601 agattatcag attattggca cagagaagtt acatttttcg gatcaggaaa cggcaaggaa
     9661 atatctgttt taatgtcact ttctttgcac aagcaaagaa aagtgacagt gacaaataca
     9721 aaatgctcgt aattaatttt ataaagaaaa agataaatca atgataaaac taaccaccca
     9781 acaaatcgct gaaatcctaa atgccaaatt gatcggcaat ggcgaaactg tggtagaaac
     9841 caccagcacc gacacccgac aagcggttga aaacggctta ttttttgcat taaagggcga
     9901 gaactttgat gctcatcact atcttcaaaa tgcagtcgca caaggttgtg tagcggttgt
     9961 ggttgagcgt gagtgtgaaa ttgatgtacc acaacttgtg gtggcagata cccgtttggc
    10021 attgggggaa ttggcgaaat ggttaaaagc taagctgaac ccgaaaacgg tagcgatgac
    10081 aggttcttcc ggcaaaacaa ccgtcaaaga gatgacggca aaaattctgc aaaaaatgac
    10141 cgcttgcgaa caggaagtgc tttatacttt tggtaactta aacaatgact taggtgtacc
    10201 gatgaccttg cttcgcttaa ccgaacagca taaatatgcg gtcattgagc tgggggcaaa
    10261 tcatatcggt gaaatcgctt ataccaccgc tatcgctcag cctgatgcct gcttggtgaa
    10321 taatgtggct gcggcgcatt tagaggggtt tggttcatta gcaggcgtgg cacaagccaa
    10381 gggcgaaatt tatcgtggct tgaaagaaaa tggcaaagcg attgtcaatt tagcttttta
    10441 ttatccgcaa tggcaaaaag agattggctc acgggaaatg caatctttct cttatatcgg
    10501 gtcagattcg gattcttacg cagattactg ggcggaagat gtagaactgc atttaagcgg
    10561 ttcacgcttt acgctacata ccccacaggg cgaaattgct attgatctgc cttatttggg
    10621 caaacataac gtgagcaatg ccttagcggc aacttcgctg gcgatgtctg ttggggcaac
    10681 gctagaagca gttaaggcag ggcttgagca gcgttcaatg gtaaaagggc gtttgtatcc
    10741 ggtgcaaatt aacgaccact gtttgtttat tgatgacact tacaacgcca atgtggattc
    10801 aatgaaatcg gcgatttctg ttttggaaaa ttatccggct tatcgtattt ttgcagtggg
    10861 tgatatggcg gaattgggtg aagaaagtag caaatgccac caagaagtag cggattttgc
    10921 caaaaatgca gcattagatt taatcgtgag ctttggaaaa gaaagtgcgg taatcagcca
    10981 acaggcagaa catcatttta ccgataaaca gcaaatggtt gatttcctac tgccgattat
    11041 tttgcaaaaa atcgaacaaa aacaaccgct tgtattattg gcaaaaggct ctcgtagcca
    11101 gaaaatggaa accgtgattt ccgaattgtg taaaccgttt aacctaaatt tctaattatt
    11161 tttaaggact aagcaatgtt agtttggctt gcagaactat tggttcaata taacacagcg
    11221 tttaacgttg tctcttatat tactttccgt gccattatgg cgttattaac tgcaatggga
    11281 atcgggcttt ggatcgggcc gaaagtaatt cgccgtttgc agatcttaaa atttggtcag
    11341 gaagtgcgta atgacggacc ggaaagccat ttcaaaaaac gtggtacgcc aacaatgggc
    11401 ggagtgatga ttctcgcagc gattggggtg agtacattgc tttgggcaga tctgcgtaat
    11461 tcctatgttt ggttcacgct gtttgtgcta tttggctacg gtgcagtggg ctttgttgat
    11521 gattatcgta agatctctcg taaaaatacc gatggtttga ttgcccgttg gaaatatttt
    11581 tggttatctg ctatcgcatt agtggcggtg tttggtatgt atgcggtggg taaagatacc
    11641 gctgcaacac aattagtcgt gccgtttttt aaagatgtga tgccgcaact cggtttattt
    11701 tatattgtgt tatcctattt cgtgattgtc ggcaccagta atgcggtaaa cctaacggac
    11761 ggcttagacg ggcttgcgat tgtgccaact attatggtcg catctgcctt tgcgttaatt
    11821 gcgtgggcaa ccggtaactt taattttgca caatatttac atattccttt tattccaaat
    11881 gccggtgaat tggttatttt atgtactgcg attgtgggag caggtttagg gttcttatgg
    11941 tataacacct accctgcaca ggtgtttatg ggcgatgttg gctcactttc gctcggcggt
    12001 gcattaggcg tgattgcagt attagttcgc caagaattat tattggtggt aatgggcggt
    12061 gtatttgtgg ttgaagccct ctcggtgatt ttacaagtag gttcatacaa attacgccaa
    12121 aaacgtattt tccgaatggc tccaattcac caccattttg aactcaaagg ctggtctgaa
    12181 ccacgggtta tcgtgcgttt ctggattatc acgctaatgc tggttttagt cggcttagta
    12241 acattgaaat tacgttaatt gattgaataa ggtgcattga gataatgtac cttatgttat
    12301 ttttaaatta cagaataaat tatgcaaaaa caatacgaaa acaaaaccgt tactattatc
    12361 ggcttaggta caacagggtt atcttgtgtg gatttcttta agaaaaaaaa tgccaatatc
    12421 caagtgattg acacccgagc aaacccagcc ggagcggata aattggacgt aactattccg
    12481 ttgcataccg gtgggttaaa cagtgaatgg ttgctgaaat ctgatcttat tgtaattagc
    12541 ccaggtttag ccgttgccac acctgaaatt cagcaagcga ttcaagctgg tgttgaaata
    12601 gtcggtgatg ttgaactgtt ttgtcgggaa gcgaaagctc cgattattgc gatcaccggt
    12661 tcaaacggca aaagtaccgt aacgacttta actaccgaaa tggcgaagca agcggggatt
    12721 aaagtgggaa tgggtggcaa catcggtgtg ccggtattaa gtttgctgga gggcgattat
    12781 gacctttttg tgctggagct atccagcttc cagcttgaga caacttattc actgaaagca
    12841 aaagctgcca ctgttttaaa tattagcgaa gatcatatgg atcgctacga ttcgattgcc
    12901 gactatcgcc aagcgaaatt gcggatttac gataatgcgg aaaatatcat cgtaaacggt
    12961 gaagatgccc aaacttaccc gatacaagcg gtcaaaaaag tgattcgttt tgcagaacaa
    13021 aatgcggaat atagcctacg caatcagatg ctttattcag gcgatgatgc aattatccaa
    13081 actaaacaga tgctgattag cggccgtcat aatgagatga atgcgttagc ggcgatggca
    13141 ttagcagaag cggcgaatgt tcctcgtgaa ggcattgtga aagctttgca aatttatggt
    13201 ggtttgccac accgtttcca agcggtggca accaatgatg gcgtgcgttg ggtcaatgat
    13261 tccaaagcga ccaatgtggg tagcacggta gcggcattaa acggcttgaa tattgccggt
    13321 acactttatt tacttttagg tggtgacggc aaggctgcgg atttttccga actcaaacca
    13381 ttaattaata agccaaatat tgtctgctat tgtttcgggc aagatggagc ggcattggca
    13441 gaattaagcg aacaaagtgt gttggttgaa acgatgcagc aagcaattga agcaatccgc
    13501 ccacaactga aaaaaggcga tatggtactg ctttctccgg cttgtgccag tttagatcag
    13561 tttaaaaact ttgagcaacg tggcgaggta tttatggcat tagcacaagg acagcacgca
    13621 taatgttaga aagaattaag tcggaatggg ataaatgggt cagattaacc ccttctaatt
    13681 cactttatga cagaactcta atttggctat ttttaggcct gctaattatc ggttttgtga
    13741 tggtaacatc tgcttcactc ccagttagca caaggttgaa taacgatcca ttttattttg
    13801 cgattcgaga cgggctgtat attattgctt caattatctt ttgttatgtt tttgtacaaa
    13861 tcccaattga aaaatgggaa aagcataact tggcactgtt ttttatttca atcggttttt
    13921 taatcgccgt actcattttc ggccgctcaa ttaatggtgc agtgcgttgg attccgttgg
    13981 gtattctcaa ctttcagccg gcagaattag ccaaattagc ggtcatttgc tattttgcca
    14041 gtttctatgt acgtaaatat gatgaaattc gtaaagaaaa agccagtttc tggcgaccgg
    14101 cagtgatttt attcttattc ggcttcttgc tgattttgca gccggattta ggtagtacct
    14161 ttgtattatt tgttctcact ttctcaatgc ttttcattgt gggggcaaaa attatgcaat
    14221 ttatgttttt aggcgtggtg ggaacggtgt tatttgcggt gttgatttta acctcggaat
    14281 accgtttaaa acgtgttacc tcctttatgg atccgtttgc tgatgcttat ggcgacggtt
    14341 tccagctctc gaactcacaa atggcatttg gacaaggtga attttgggga caaggtttag
    14401 gtaattcggt acaaaagcta gaatatttgc cagaggcaca tactgacttt gtaatggcgg
    14461 ttattggtga agaatttggt ttctttggta ttgcttgcat cgtactttta cttattctat
    14521 taacggtgag agccttaaaa atcagtaaag aatctttggt gctggaagag cgttttaaag
    14581 gctatatggc atttgggatt gcgatttggg ttttcctaca aggcttcgta aacttagggg
    14641 tggcatcagg tttgctgcca acgaagggct taaccttccc attagtgagt tatggtggtt
    14701 cgagtttggt aattatgtcg attgcgattg cagttttgct acgaattgac cacgaaaacc
    14761 gtgccgagcg aatcgggcac gcaaaaatta aaaccatcta gccgagcggt ctgcaacgtt
    14821 acaatttgca aaattttaca aaaatgagac cgcttgtatt agctggaaag cataaaaatt
    14881 cagtaaaata gcacgtttta gaataaaaaa gagttgaaaa tggcgaagaa attattagta
    14941 atggcaggtg gaacaggggg gcacgttttc cctgcaattg ccgtagctcg agagctacaa
    15001 aaacaaggct ggcaaatccg ctggctgggg acgaaagacc gaatggaagc cactcttgtg
    15061 ccgaaacacg gtattgagat tgattttatt gaaatctcag gcttgcgggg taaaggcgtt
    15121 gcagcattgt tgaaagcacc gtttgctatt tttaaagcgg tgatgcaagc ccgcaaaatc
    15181 attaaaaatt atcagcctga tgcggtgttg ggaatgggcg gttatgtgtc cggtccgggc
    15241 ggtattgcgg ctaaattatg tggcgtgccg gtgattttac acgagcaaaa cgcagttgcc
    15301 ggcctaacca atgtgtggct atccaaaatt gcccgcaagg tgttacaggc tttccctact
    15361 gcttttaaag aggccgaagt agtaggtaat cctgtacgtg aagatttatc tgctcttccg
    15421 ctgccgaatg aacgtttcgc caaacgaggc tacccgatta acattttagt aatgggtggc
    15481 agccaagggg caagagtgat caaccaaacc gtgccggaag tggcaaaagt gttgggtaat
    15541 aatgtgttta tcagccacca agtcggtaaa ggtaatttag ccggtatcga agaagtctat
    15601 caacaaaccg gcaatgggat tgcttccgag tttattgatg atatgaaagc cgcgtatgag
    15661 tgggcagatt tggtgatttg tcgttccggt gcgttaacgg tgtgtgaaat tgccgccgta
    15721 ggtctgcccg ctgtgtttgt gccgttccaa cataaagaca ggcaacaatt tttaaacgct
    15781 aactatttgg cggcagatgg agcagcagtg attatcgaac agagcgattt cactccaaag
    15841 gcattacagc aagccttaga acccttaatt gccaatcgcc aactcttatt ggatatgtca
    15901 atgcgagcca gagcaaaatc caccccaatg gcagcaaaac gtgtagcgga agtgattatt
    15961 caagaagcga attaacttac tacaagcggt cgtttttggt aaaaattttg caaaatgtaa
    16021 ccgcttgcga atagattaaa aggacaataa atgaaaaatt tccaagataa agtcaaaaaa
    16081 ttagtgcctg aaatgcgtcg tgtcagccaa attcatttta tcgggattgg cggagccggt
    16141 atgagcggca ttgcggaagt gttattaaac gagggatacc aaatttcagg ttcggatatt
    16201 gcagacagag cggttacaca acgtctatcc gcagccggag cgaaagtctt tatcggacac
    16261 caagcagaaa atattgctgg ggcgagtgtg gtggtggttt ctactgcgat tgatgaaacc
    16321 aaccctgaaa ttattgccgc tcgagaagct cgaattccgg tgattcgccg agcagaaatg
    16381 cttgccgaaa ttatgcgttt tcgccacggc attgcgattg caggcacaca cggtaaaacc
    16441 actacaaccg caatgatttc aatgatctac accgaagcaa aattagatcc gactttcgtg
    16501 aatggcggtt tggtaaaatc taccggcaaa aatgctcatt tgggggcgag ccgttattta
    16561 attgccgaag ccgatgagag tgatgcttct ttcttgcact tgcagccaat ggtgtcggta
    16621 gtaaccaaca ttgagcctga ccatatggac acctatggtg gcgattttga gaaaatgaaa
    16681 gagacttacg tacgattttt gcgtaactta cctttctacg gcttagcggt aatgtgtgcc
    16741 gatgatgaaa cggtaatgga aattgctcct cgtgtcggca gacaagtgat tacctatggc
    16801 tttagcgaaa atgcggatta ccgaattgaa gattaccagc aaaccggctt ccaagagcat
    16861 tacaccgtag tttgcccaag cggtgaacga attgatgtgc tgttgaatgt gccgggtatg
    16921 cacaatgcct taaatgcaac cgctgcgtta gcggtggcaa aagaagaagg gattggcaat
    16981 gaggcgattt tagctgcttt agctgatttc caaggggcag gacgccgctt tgaccaatta
    17041 ggttcattta ttcgcccgaa cggcaaaatt atgttagttg atgactacgg ccaccaccca
    17101 actgaagttg atgtaaccat tcaagccgct cgttcaggct gggaaggtaa acgtgttgtg
    17161 atgattttcc aaccgcaccg ctattctcgt acccgtgatt tattcgatga atttgtacaa
    17221 gtactgtcta aagtcgatgc cttgatttta ttggatgtgt atgctgccgg cgaagctccg
    17281 attgtggggg cagacagtaa agccttagcc cgttcaatcc gtaatttagg taaggtcgat
    17341 ccgattttgg tttcagatac cgatcaactt ggcgaggtat tagatcaaat tattcaagac
    17401 ggtgatttaa ttttggcaca aggtgcgggc agtgtaagcc gcatttctcg tggcttggct
    17461 gagagctgga aaaattaaat actctctccc cttgcgggag agagaccgaa aaatacttcg
    17521 ttcagaagta tttttcagag agagggtata tatttaaagg atttcaaatg agtattaaaa
    17581 acgaaaaaat tgccgtctta ttcggtggcg tttcgcaaga gcgtgaagtt tcactgaatt
    17641 ctggtgcagc ggtaacagct gcattaaaaa gtttaggcta taacgtagaa ggcattgata
    17701 ctaaagaatt cccgattgaa aaattaaaag aaaaagggat tcagcgtgta tttaatattt
    17761 tacacggcgg tattggcgaa aatggcgtac tgcaaggtgc gttagagcaa atgggtattc
    17821 cttacaccgg ttgtggcgta atggcatcag cagtgacgct ggataaattc cggactaaat
    17881 tattatggaa tgcagtaggt ttaccaacag cggatatggt ggtggttcag cgtggacaag
    17941 cggtcgattt tgaccaaatt attgcaaaat taggcttacc ggtatttgtg aagtcttcgt
    18001 gtgaaggctc aagtgtgggt gtattcaaag taaaaaccaa agaagaattg gcaccggcaa
    18061 ttcacgaggc attaaagttt gattccattg tgttggtgga agaattttta gccggtaatg
    18121 aatattctgt accggtatta gacggacaag ttctaccggc ggtacaagta attccggacg
    18181 gcgaatttta tgactaccac gcaaaatatg tgtcggataa cacccaatat gtagtaccgg
    18241 cattaaatga cgaacgccaa gcagaggtcg ccaaattagt gaaagcggct tacgatgtgg
    18301 ttggctgtcg aggttggagc cgtattgatg tgatggaaga tggcgaagga aaattccgct
    18361 tggttgaagt aaataccaac ccgggaatga ccagtcacag tattttccca aaatcagcag
    18421 caacagttgg tatttccttt gaaaaattag ttgaacgcgt attggagtta agtgcttaat
    18481 gggcatcttc cgcaagaaga acagcacaaa tgtgcgtttg aatgttctta atccaaaaac
    18541 acctgtttct ccacgtaaat ggctagtatt cattaagcca ttaattgtgt tattatgtac
    18601 actatttgta ttcggtgtgt atagtaattg gcaaaatata ctcgaaagtt gggataaaac
    18661 accgattcgc gcttatgcat taacacataa aacacaattt acaacaaatg cagatattcg
    18721 tgaaactttg tctaaagaac ctgctctaaa aggatatttc ggacaaaata ttcaagaggt
    18781 caaagataag cttttatcta taccttgggt taaagatgtg gctgttcgta aactttatcc
    18841 cgaccgatta agtattactt tgctagaaca taaacccgtt gcaatttgga atgatactaa
    18901 ctttgtctca gaacaaggaa ccgtctttag tttaccgaaa gacagaatcg ataaaaatgg
    18961 acttccatta ctctatggtc cagatacgga aggaaaaagt gtattagagg catggaataa
    19021 aatccgagct gatctaaatg cccgcaactt ggatttaacc tccgtttctg tagataatcg
    19081 aggttcttgg acaattaggc tttctaatca agtagaatta agattaggaa gaggggaatg
    19141 gacttctaaa attgatcgtt ttgtaataat ctttcctgaa attcatatac ctgaaggtaa
    19201 tagattagct tatgttgatt tacgttatcc acatggcgcg gcggttggat ttagtcctat
    19261 aaaaaattaa actcagtgaa cacaaaaaca ttatgacaaa agcagtagaa tcaaaaatta
    19321 tcgttggtct tgacattggg acatcaaagg ttgttgccgt tgttggtgaa gtattacctg
    19381 atggtgtaat taatgttatg ggagctggcg tttgcccttc taaaggagtc gatcgaggtg
    19441 gcgttattga tcttgacgct gccgttaatt ctattcaaag agccattgag caggcggaat
    19501 ctattgccga ttgtcaaatt atgggtgtaa cactggcaat ctcaggtcag catattgttg
    19561 gtttaaatga agcaggatca tcaacattat ctggaggtat tgttacccaa gataatattg
    19621 aaagtgctgt ggatatggca aaggcaatta aattacaaga tggcttagaa acattacaca
    19681 tgattccaca agaatatcac gtggatagat tgcctgcgac taaaaatcca attggcttat
    19741 cgggtatgcg tttacaagtt caagcacact taattgcttg ccatcaagct tggttaaaaa
    19801 acttaaaaaa tgcggttgaa catgctaaat taaaagtcga tcaagttgta ttttcaggtc
    19861 ttgcctctag ctattctgtt ttaactgaag atgaaaaaga gctgggtgtt tgcttaattg
    19921 atattggtgg cggtacgatg gatgtactag tttataccga tggagcatta agatacagta
    19981 aagtcattcc atttgcaggt aataacatta ctgattattt ggctcgtgta tttacgacat
    20041 ctcgccctga agctgaaagc cttaaagtag gttatggtag cgcaatttcg cctccaaccc
    20101 ataattcaga taaaaaaatt gaggtggcag gactaggtgg acggatggca agaaccttta
    20161 cccgagctca ggtggcaacg gttacctcac aatgttataa cgatttgcta aaagttgttg
    20221 aagaggaatt aacccaatta cgccatgaac ttttccaaaa aggaataaaa caagagttaa
    20281 ttgccggtgt agtcattact ggtggtggct cacaaattga agatattgta gaatgtgcta
    20341 aatcaatttt tggttcacaa gttcgtgttg gatatccact caatattact ggcttaaccg
    20401 attatgtgaa taaaccacaa tatgcaacag cattagggct tttacaatat agccactata
    20461 attatactga acaaggtaca agtacagatt ctaatgaaga tatctttgct cctattggtc
    20521 gtggaaccaa gaaaatcatt gacttcttca aatcaaaatt ttaatagtgt taaaataaac
    20581 acgcagggag ataaaaatgt ttgaacctat tattgaacaa acgcaagatg ctatcattaa
    20641 ggtagtgggt gttggtggcg gtggcggtaa tgccgtagac agaatgtctc gctctgccga
    20701 tgatattaaa ggtgttgaat tctttgatgt aaatactgat gctcaagttt taagaaaaag
    20761 aaccacacgc caaactatcc aaattggagc aagtacaact aaaggtttag gtgcaggtgc
    20821 tgatccaatg gtaggaaaac aagctgccga agaagatcgt gaggctatcg ctaatgcatt
    20881 aaaaggtgca aatatgacct ttattgctgt tggtatgggt ggaggtaccg gtaccggagc
    20941 agcccctgtt gttgctcaaa ttgccaaaga gcaaggctca ctaactgtag gcgttgtaac
    21001 aaaacctttc cgcttcgaag ggcctcgccg tatgcgcttt gcagaccaag gcattaaaga
    21061 gctttctcaa tatgttgatt ctcttattat aattcctaat gataaattac gtggtttagg
    21121 taagcaaaca acagcagtag atgcctttgc cgcagcaaat gatgttttaa gtaactgtgt
    21181 gcttggtatt accaatatga ttaccagttc gggcggttct accggtgcag atattaacgt
    21241 ggacttcgcg gatgttcgta cagtaatgtc tggtaaggga cacgctatga ttggtacagg
    21301 ttttgctgaa ggtgaagttg gtgaaggacg agcggaaaaa gcaatgaatg atgccatttc
    21361 tagtccatta cttgaaaatg tggatatttc aggtgcaagt ggtatgctca tcaacattag
    21421 tgctgggaca gatttcttat tagaagaagt ttatgctatg atggacttaa tttatggctt
    21481 tgcaacagaa gatgccgcaa ttgtatttgg ttgtaactat taccctgaaa tggatggaaa
    21541 agtgagtgtc actcttgtcg caacaggaat aggccaacct gaggaagctc tacatatgcc
    21601 acataaggct cagccagtat atgcacagca aggaaatcat gttcaagcta cacagcctac
    21661 tcagccaaac catgcggtac aacaaccaaa ctttactcaa ccgtcaagtt atggtcagcc
    21721 aaatcaattt agtggtcaac cacaaccact acaacaacct gtggctccaa aaccacaatc
    21781 ggttgataca gcacaaattt ttaacccaaa ttcaattccg ggttttatga gaaatagtca
    21841 ataacttcca ctcatttcgg aagtaaggac aaaatatgac taaacaaaga acattaaaac
    21901 aagcagcaaa agtaacaggt attggcttac atagcggcaa aaaagtaacc ttaacgttac
    21961 gccctgcccc agctaataca ggagttattt atgcccgtac cgatttagag cctgtcgttt
    22021 atttccctgc aagcgcagac tctattcgtg atacacaatt atgcacatgt atggtaaatg
    22081 atgatggtgt tcgtatttca acagtagaac atttaaacgc agcaatgtca gcattaggct
    22141 tagataatgt tattgttgaa gtagatgcac ctgaaattcc gattatggac ggtagttcaa
    22201 gcccatttat ctatctttta ttagatgccg gcatcgaaga acaagatgca ccgaagaaat
    22261 ttattcgcat taaagaaaca gtacgcgttg aagaaggcga taaatgggcc gaattaaaac
    22321 catacaataa aggttttaaa ttgaacttca ctattgactt tgagcaccca ttaatcccca
    22381 aaaatgtagg gagctattcc attgattttt ctgctgagaa ctttattcag caactaagtc
    22441 gagcgagaac ctttacattt atgaaggatg tagaatactt gcaatctatt ggtcttgcat
    22501 taggtggaag ccttgataat gctattgtat tagatgatta ccgaattcta aatgaagacg
    22561 gtttacgatt taaagatgaa ctggttcgcc ataaaatgct ggattcgata ggtgacttat
    22621 atatgtgtgg ttataatatc ctaggtgaat ttagtgcata taaatcaggt catggcgtta
    22681 ataataaatt attaagagct gtattggaaa ataaaaacgc ttgggagttt gttacctttg
    22741 aagataaagc agaagtccca caaggttacc aagtacaaca agaaattttt atttagtcta
    22801 aatggcttaa aagacgaaca gaaatgttcg tcttttattt tgcaaatttt ttctaatatt
    22861 taaccgcttg caacccgcct cattagcgat tttttcgccc ttatctattt actgtttaaa
    22921 aaaacagttg tactatatac ccatacagtt aatcttgtgt ggtgtattat gcttactcaa
    22981 cttactatta ataattttgc gattgttcgc catcttattc ttgaattaaa tgaaggtatg
    23041 tctgttatta ccggtgaaac cggtgccggg aaatcaatcg ctattgatgc cttagggcta
    23101 tgcttgggtt atcgttctga aagtgggatg attagaaacg gttcggacaa agcagatata
    23161 agtgcaacct ttactatgca gccacatagc cctgcttatt tatggctaca agagcatgaa
    23221 ctgcttgatg aagataatcc tcaagagtgt atcttacgcc gtatgattaa tattgatgga
    23281 cgctcaaaag cctttgtaaa taaccgatca ttacctgttt cacaacttcg agagttgggg
    23341 caatatctta ttcatttaaa tgggcaacac gcaccacaat tactcctaaa aaacgaatat
    23401 caacttgaaa tagtcgatca ttatgccacg ttacagcctc tattgatgaa gatggcaaag
    23461 caatatggga attggaaaga actgaataag caggtaaaaa atttccaaaa acagtgccaa
    23521 gaaaatgaag ctcgcaaaca gctattagaa tatcaagttg aggaattaga tgaatttgca
    23581 attaaagcaa acgaatttga agaaatggaa gagaaataca atatcttgtc ttcttccgag
    23641 gagcttactg agctttcaga tgaaatcaca cgcttattaa gtgatgatga aatcaatgca
    23701 aacagtctga tttataaagc gttacgcaat gcagaaaact tggctgaggt tgatagccaa
    23761 tatcagggca ttttagatat gcttaatgag gccctgattc agattcaaga agcaagctcc
    23821 gaagtggctc aattagccag taaaattgaa caagatccgg aattactaca ggagctggat
    23881 aatagaattg cacaaactat atcgttagca cgcaaacacc atattcaacc gaatgaatta
    23941 tggcaatatc acgcgacatt aaaagcagaa ctacaggaat tactggattt tgaagataat
    24001 gaagaacaac tcattcaaaa tgaaaaacaa gcttaccaag agtgtataac attagcagaa
    24061 gaaatttacc aaaaacgtct agatgccaca cataagctct ctgaacaagt aacaaaacaa
    24121 attaaacact tagcaatgga aaacggtgag ttctttattc atatagaaca taatcttgaa
    24181 aaaatgtctg caaatggtgc tgactatgtc gagtttaatt tacgcagcaa tttaggacaa
    24241 caaccccaac cactggcaaa aattgcttct ggtggtgagc tatctcgcat ttctctggca
    24301 gtacaagtgc ttaccgcaaa taaactttct acgccaacta ttatttttga tgaagttgat
    24361 gttgggatta gtggctctgc tgccactaca gtcggaaagc tactgcgtaa gctaggcgaa
    24421 aaatgccaag tattatgtgt aacccattta cctcaagtgg caagttacgg caaccagcag
    24481 tttaatgtac aaaaatatgt agaaaataat caaactgaaa cgcaaatgac attactcaca
    24541 gaagcagagc gtattcacgc cttggctcga ctattaggcg gaagcgaaat caccgaaacg
    24601 gttttagcta atgcaaaaga aatgctaaat ttagcgaaaa ataacgaata gccaaaaaga
    24661 aaaatcgcat caatgaatat tgatgcgatt atgaatttgg tggaggtaaa cgggatcgaa
    24721 ccgatgacct cttgcatgcc atgcaagcgc tctcccaact gagctatacc cccttaatac
    24781 aagcggaata atatgctttc cgcttgaaga tgtcaatggc tttaatcaag caaggtgttg
    24841 attgattaaa aactagctat tcgcctgaat aaactcaatc gctttttgta agcgagctaa
    24901 ggttctttct ttaccgacta atttggcggt gatatccatt gacggcgatt gcccacttcc
    24961 tgttaccgct aaacggaacg gcataccgac tttgcccata ccaatttcca gctcggcagc
    25021 ggtttggttc attgcctcgt ggatattttc caccgtccag tcattgcaag cggtcaattt
    25081 ttccaataat tttgcaagtg gagcaacggc ttcggctttg aagtttttag cagcagcttt
    25141 ttcgtcgtaa gccttaaatt cttgatagaa atagcggctg ctttcggcaa gctcttttaa
    25201 ggttttgcaa cgttcgccca gtacagggat aatttcttcc aattttggac cgcttgtgaa
    25261 atcaatgcct tgagtttgca tatgccattc aagatgtttt gccacataac ttggggcaag
    25321 actgcggata tagtgttggt tcaaccattg taatttttca gtgttaaatg cacttgccga
    25381 tttactgact gaatgaatgt cgaacagctc gatcatttcg tcacgagaga aaatctcttg
    25441 gtcgccgtgt ccccagccta aacgcacaag gtagttaatt aaggcttccg gtagatagcc
    25501 gtcatcacga tattgcatta cgctcaccgc accgtggcgt ttggagagtt tttgcccgtc
    25561 atcgccgttg atcattgaaa cgtgggcgta ggttgggatt ggtgctccta atgctttcaa
    25621 aatgttgatt tggcgtggtg tattgttgat atggtcttca ccccgcacaa cgtgagtaat
    25681 gcccatatcc cagtcgtcca ccaccacaca gaaattgtag gtcggcgagc cgtcagtacg
    25741 gcgaataatc aagtcatcta attcgctatt ggcgatttca attcgcccac gcaccgcatc
    25801 gtcaaatact accgagcctt cggtcgggtt tttgaaacgc acaacatgtg gttggctcgg
    25861 gtcgttttgc ggattgtgta agcagtgctg atcgtatttc gctttctctt tatttgcttc
    25921 ttgctctgca cgtacttcgt ctaaacgttc tttcgagcag tagcaacggt atgccgtgcc
    25981 ggcttctaac atttggtcga tcacttggtt atagcgatcg aaacgcttgg tttgaaagta
    26041 tgggccgtgt tcccacgcca aatccagcca ttccataccc tctaaaatag cggcggttgc
    26101 ctcaggggtt gagcgttcaa gatcggtatc ttcaatacgt aacacaaatt caccatcatt
    26161 atgcttagca tataaccaag agtagagcgc ggtcctcgcc ccacctacgt gcaagtagcc
    26221 agttgggcta ggtgcaaaac gggtacggac ttttacattc ggatctaagg ggaaaagggt
    26281 ttcaattttc attttatatt gcctaattga tgattcttaa gaaaaacttg tctattttac
    26341 tacgcttgag ggtgaatcat agtaaaaaat taaatttgcg gggtaaaaaa tcagcaaacg
    26401 gggtaaaaag aacaaaaagg gtttgacgaa acagcaaaaa tctctataat tcacttccac
    26461 aacagcgggc gattagctca gttgggagag cacctccctt acaaggaggg ggtcactggt
    26521 tcgagaccgg tatcgcccac cacttcaact taagtgtttc gttgttgtat cacaatctaa
    26581 ttttgaaaac gggcgattag ctcagttggg agagcacctc ccttacaagg agggggtcac
    26641 tggttcgaga ccggtatcgc ccaccacttc aacttaagtg tttcgttttc aaattccctt
    26701 tgattttaag tctcaagtgg gcgattagct cagttgggag agcacctccc ttacaaggag
    26761 ggggtcactg gttcgagacc ggtatcgccc accacttaag actaaaatca taaacttcaa
    26821 atggcgattt ttcgtatctg aagggcgatt agctcagttg ggagagcacc tcccttacaa
    26881 ggagggggtc actggttcga gaccggtatc gcccaccatc tatttagtat caaagcctaa
    26941 aacttatatc ataatctaat aaccccttta aagtcataac taaaaaggga aaataaattc
    27001 agatggcctg ttgcatctgc ataaatgcct ttctacgtgc agaaattaat ctttttctat
    27061 ctttaatttg cttaatttga cgcaggcgta atttccttgt cagtagggat ttctttttct
    27121 tcaattttct cataagaaga attctcctgt tttatttgac catctggtaa attttcttgt
    27181 tcttgcatct tttgctcttc aactcgttga taattaataa tactaccata ataacgccgt
    27241 atattttcaa catactgtaa tgcttcatat cctcgagcat agccgtattt tagattagca
    27301 taataacgtt tttccgcaag caatggcaga ttctttttaa catcaagcca attatccgga
    27361 tcaccaccaa gttgttttgt taatcgacga agatccaaca agtgccccaa tcccatatta
    27421 tacgctgcta ggctatacca aattctatct gcttgaggaa tagtttccgg catttgtctc
    27481 attagcatat gtaaatattc agatcccgct ttgatacttt gtgcagggtt agtcctatca
    27541 gaaattttca ttctatctgc cgtatctttt gtcagcatca taattcccct cactccggtt
    27601 ggagaagttg cattaggatc ccaatgagac tcctgatagg cgatagccgc cagcatttgc
    27661 cattctaatt cacctttatg tgcccgaaaa aggggttcat atttaggtaa aatagtttta
    27721 attgcattca aatagctctg aatatcaaca taatcaaact tagctaaatg attaaaatat
    27781 ttctcttcta ttcttgatat cagcccagtt tccatagaat aagacataaa atccagcagc
    27841 acagattgca gttcactata agaactattt gctaaatacc ataacactgg gttttcatca
    27901 gttaaatcaa accctacggc taaattagga ttaatatgct gtgcagcaga aatatcaacc
    27961 gacattgcaa ttgtatgtga aatttcacct tgtgcgactt ttaacaatag ctcttcttgt
    28021 gtaaatttat ccgagatctg ccattttagt tccggattat ccaccttaag ctgatttaat
    28081 attggaatta ccgcagaacc ggctggaata atcacatttt cccgcaaatc ggaaagtcga
    28141 tatggtctag cacttccctt tttataaaca agctgccaag aagccgaata ataggaagga
    28201 cctatctgaa attcatcgtt taattcagaa tggtaaagca gcccagcggc agcaatatca
    28261 acatcattat ttcttaaggc ctcaaacaat ttctcgctac tatcataagt tttaatatca
    28321 agattgacct ttaaatattg agcaaaggct gttgctaaat catattcaat gccacttttt
    28381 ccttcaggac caacaaagta agaaagtgga tggttgatca ttccaacctt taacgttttg
    28441 ctttgctgaa tttgtgtata atgatttgct tctgaatgaa tgattttttg ccaaggaaaa
    28501 atcatatccc aagcccacag taacaatgct acggcaataa aaaggcgagc aattaatcct
    28561 ttcaatttaa aatcctcatt atttcaagct aagcattaag cctatctgaa attacaaatt
    28621 aacggaataa tactttatag tttaattatt tattaa
//
LOCUS       DANLXQ010000013        28527 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.013, whole genome shotgun sequence.
ACCESSION   DANLXQ010000013 DANLXQ010000000
VERSION     DANLXQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28527
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.013"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            1..228
                     /locus_tag="PXD94_000448"
     CDS             1..228
                     /locus_tag="PXD94_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDL6285345.1"
                     /translation="MKMVEHCVKELLEQLTATLEEGERIEVRGFGSFSLHYRQPRLGR
                     NPKTGESVLLGAKYVPHFKAGKDLKERVDLL"
     gene            265..552
                     /locus_tag="PXD94_000449"
     CDS             265..552
                     /locus_tag="PXD94_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL6285346.1"
                     /translation="MIKYILGILIVIAVIIVAVTIGANNDQLITFNYIFAQSELRLST
                     LVAILFGLGLILGWLVTGIFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            556..1752
                     /gene="lapB"
                     /locus_tag="PXD94_000450"
     CDS             556..1752
                     /gene="lapB"
                     /locus_tag="PXD94_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL6285347.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQDPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            1776..2468
                     /gene="pyrF"
                     /locus_tag="PXD94_000451"
     CDS             1776..2468
                     /gene="pyrF"
                     /locus_tag="PXD94_000451"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL6285348.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            2551..2862
                     /gene="yciH"
                     /locus_tag="PXD94_000452"
     CDS             2551..2862
                     /gene="yciH"
                     /locus_tag="PXD94_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL6285349.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            2965..5127
                     /locus_tag="PXD94_000453"
     CDS             2965..5127
                     /locus_tag="PXD94_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL6285350.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELLEYAQ
                     NVKQSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGLKTDEKMRLKAVELIKQNANAELANLAANVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYADNKAIIQQLFAKYVRTLSENMENPSFQQYQT
                     WAEKYQLSSEEINGWKNAFITRFFDNPEPTFQFWRDEQIKLLKTDNLTERRLRMAIWQ
                     KADLNEWLVLLSQQAKEKAEWRYWAAKEEKNEAKRKVDFESLAKERGFYAMLAAQQLG
                     IVYQIPMLEVPELTQAQLELYANQLARIQELRELGQFDQARKIWIDWIKNIKPEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQTKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMLYSDINDKNGLKMFYQEELTRKY"
     gene            5176..5252
                     /locus_tag="PXD94_000454"
     tRNA            5176..5252
                     /locus_tag="PXD94_000454"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5210..5212,aa:Met,seq:cat)
     gene            5479..5940
                     /gene="rimP"
                     /locus_tag="PXD94_000455"
     CDS             5479..5940
                     /gene="rimP"
                     /locus_tag="PXD94_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL6285351.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDRQFAFGNIQKANLVPVFNF"
     gene            5961..7457
                     /gene="nusA"
                     /locus_tag="PXD94_000456"
     CDS             5961..7457
                     /gene="nusA"
                     /locus_tag="PXD94_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL6285352.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            7442..10009
                     /gene="infB"
                     /locus_tag="PXD94_000457"
     CDS             7442..10009
                     /gene="infB"
                     /locus_tag="PXD94_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL6285353.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
     gene            10094..10474
                     /gene="rbfA"
                     /locus_tag="PXD94_000458"
     CDS             10094..10474
                     /gene="rbfA"
                     /locus_tag="PXD94_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL6285354.1"
                     /translation="MSREFKRSDRVAQELQKEVAVILQREVKDPRIGMVTVSDVEVSR
                     DLAYAKIFVTFLFDNDAEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESL
                     VEGMRMSNLVSSVIKNDEEKHKED"
     gene            10477..11400
                     /gene="truB"
                     /locus_tag="PXD94_000459"
     CDS             10477..11400
                     /gene="truB"
                     /locus_tag="PXD94_000459"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL6285355.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            11403..12483
                     /locus_tag="PXD94_000460"
                     /pseudo
     CDS             11403..12483
                     /locus_tag="PXD94_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
     gene            complement(12521..13045)
                     /gene="tadA"
                     /locus_tag="PXD94_000461"
     CDS             complement(12521..13045)
                     /gene="tadA"
                     /locus_tag="PXD94_000461"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL6285356.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVHGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(13127..14935)
                     /locus_tag="PXD94_000462"
     CDS             complement(13127..14935)
                     /locus_tag="PXD94_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL6285357.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSANSASNANPFEGRGRR
                     GNDRNDRGGRRDGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            complement(15137..16060)
                     /gene="nlpI"
                     /locus_tag="PXD94_000463"
     CDS             complement(15137..16060)
                     /gene="nlpI"
                     /locus_tag="PXD94_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL6285358.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            complement(16141..18291)
                     /gene="pnp"
                     /locus_tag="PXD94_000464"
     CDS             complement(16141..18291)
                     /gene="pnp"
                     /locus_tag="PXD94_000464"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL6285359.1"
                     /translation="MTPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVV
                     AKKDVKEGQDFFPLTVDYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLF
                     PEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
                     VLNPTTSEQKISRLDLVVSGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENI
                     KEFVKEAGKPRWDWVAPEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEV
                     IAQLTAEDETVSEGTIIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLP
                     RTHGSALFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRI
                     GSPKRREIGHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALM
                     DAGVPIKAAVAGIAMGLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDI
                     KIEGITPEIMRIALNQAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKD
                     VIGKGGATIRALTEETNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIY
                     NGKVTRVVDFGAFVSILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQG
                     RIRLTMKDINNTNEANAEETVAENVVETEQENNF"
     gene            complement(18553..19833)
                     /gene="phoR"
                     /locus_tag="PXD94_000465"
     CDS             complement(18553..19833)
                     /gene="phoR"
                     /locus_tag="PXD94_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL6285360.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
     gene            complement(19836..20522)
                     /gene="phoB"
                     /locus_tag="PXD94_000466"
     CDS             complement(19836..20522)
                     /gene="phoB"
                     /locus_tag="PXD94_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL6285361.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLMTHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            complement(20606..21376)
                     /gene="pstB"
                     /locus_tag="PXD94_000467"
     CDS             complement(20606..21376)
                     /gene="pstB"
                     /locus_tag="PXD94_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL6285362.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            complement(21386..22234)
                     /gene="pstA"
                     /locus_tag="PXD94_000468"
     CDS             complement(21386..22234)
                     /gene="pstA"
                     /locus_tag="PXD94_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL6285363.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMISYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            complement(22231..23190)
                     /gene="pstC"
                     /locus_tag="PXD94_000469"
     CDS             complement(22231..23190)
                     /gene="pstC"
                     /locus_tag="PXD94_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL6285364.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            complement(23271..24308)
                     /gene="pstS"
                     /locus_tag="PXD94_000470"
     CDS             complement(23271..24308)
                     /gene="pstS"
                     /locus_tag="PXD94_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL6285365.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKINYQSIGSGGGQQQIIAKTIDFGASDDPMKGETLAEHKLLQFPAIIGGTV
                     PVVNIPELKSGELKLSGKVLADIFLGKITQWNDPAIVALNQDIALPAKTILVVRRSDG
                     SGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPVGQGGKGNEGVAAYVKQLKYSIGYVEY
                     AYAKQNALAWVSMQNADGKFVQPSRETFAAAAANADWSQTAGMGVILTNEAGEKAWPI
                     TAASFILIHQHSDKPEQTKGVFKFFDWAFKHGKDAAAELDYVPLPDNVVEKIQTQWKT
                     EVKDGQGNSLY"
     gene            24493..26739
                     /gene="parC"
                     /locus_tag="PXD94_000471"
     CDS             24493..26739
                     /gene="parC"
                     /locus_tag="PXD94_000471"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL6285366.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYKAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQGP
                     DYPTEAEIITPKAEIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQVA
                     TQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVNM
                     NMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLHILDGLMIAFLNID
                     EVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKERD
                     ELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPTEDVTVI
                     LSEKGWVRCAKGHDIDVQALSHRAGDGYLAHARGRSNQPVVFLDSTGRAYALDPTSLP
                     SARSQGEPLTGKITLPEGAVVQQVLMASPETKVLMASDSGYGFICTFADLVSRNKAGK
                     AVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPQLSKGKGNKIINISA
                     AAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDVDNYRGERARKGSQLVRGLST
                     NSTVEIVE"
     gene            complement(26810..28162)
                     /gene="lysC"
                     /locus_tag="PXD94_000472"
     CDS             complement(26810..28162)
                     /gene="lysC"
                     /locus_tag="PXD94_000472"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL6285367.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
BASE COUNT         8539 a         5583 c         6362 g         8043 t
ORIGIN      
        1 attaaaatgg tggagcattg tgtaaaagag cttttagagc agttaacggc tactttagaa
       61 gaaggtgaaa ggatagaagt aagaggattt ggtagttttt ctctacatta tcggcagccg
      121 cgtttaggtc gcaatcccaa aacaggagaa agtgttcttt taggcgcgaa atatgtacca
      181 cattttaaag ctggtaaaga tctaaaagag agagttgatc tgctttaagt cttattaatt
      241 caataatcgt agtaaggaga ttatatgatt aagtatattt taggtatttt aattgtgatt
      301 gcagttatta ttgtcgctgt tacgattgga gccaataatg atcaacttat cacctttaac
      361 tatatttttg ctcagagtga attacgctta tcaacgttag ttgcgatttt atttggctta
      421 ggattgattc ttggttggtt ggtaacgggt attttttact taaaaattaa gtttcaaaac
      481 gttgctttaa atcgtcgtgt aaagcgccaa gctcagcaaa taactgaatt aacgactcca
      541 aaggctgaat aatcaatgct tgaattattg ttccttttat tgcctattgc agcactatat
      601 ggctggtata tgggacaacg tagtgcaaag aaggatcaag aaaccgctag taataagttc
      661 tctcgtgagt atgttacggg gttaaatttt cttttatcta atcagcagga aaaggcggtg
      721 gaccttttcc tctctatgtt acaaaaacaa gaatccgaaa atcatattgc atctgaatcc
      781 caatttgaag cggagttaac cctaggtaat cttttccgtt caagaggtga ggtagatcgt
      841 gccttacgta ttcaccaaac attagtgaat agccctcatt attcatttga tcaaaaatta
      901 ttagcaaaac agcaattagc taaagacttt atggttgcag gtttctatga ccgtgcagaa
      961 aattattaca tttcattagt tgatgaacct gagtttgcta aatattcttt atctcagctt
     1021 gctgttattt accaaaaaac cagagaatgg aaaaaagcga ttaatgttac agaaaaacgg
     1081 cttcgaatag agccggatgc agataaaata ccgctttcac atttttattg tgaatatgcg
     1141 caaggggtta aatttgatga taaagcggca tttattgaat cgttaaataa agcgttagaa
     1201 tattatcctc aatccacacg cgcttctata atattaggtg atttctattt agaagaggga
     1261 gaatatctaa gagcattaag ctatttcgaa cagattcttg tgcaagaccc tgattatatt
     1321 agcgaagtgc ttggacgaat taagcaaagc tatatggctc ttgaggactt aaatggctat
     1381 gagttatttc tcattcgagc aaaccgagta aaacataata gtagtgtaga tattgcttta
     1441 actgaattta ttgaagaaaa agatggtatc aatgctgcac attctaaact ataccagcag
     1501 ttgagtactt atccgaattt aattactttt caccgcttta ttcgctatca agcagatttt
     1561 gcggaggaag gaaatggtaa ggaaagctta gttttattac ataatatggt gggaaatcag
     1621 ataaaacgca gcttccaata tcgctgttta aattgtggtt atcaaagtta tcgcttaatg
     1681 tggcaatgtc cttcttgtaa tcaatgggaa aaaattaagc cggtacaaag tatcgaaggt
     1741 attattcaat aaagaaagaa tctgaggaat tgaaaatgaa caataaagta attgttgcgt
     1801 tagactatga aactgaacgt gaagcattag aatttgtgga tttggtcgat ccgtcgcttt
     1861 gtcgtttaaa agtaggcaaa gaaatgttta caaccttagg cacaaatttt gtcaaacaat
     1921 tacacgaacg taaatttgat gtattccttg atttaaaata tcatgatatt ccaaatacgg
     1981 ttgctcgagc agtacgttct gctgctgatc ttggtgtgtg gatggttgac ttgcatgctt
     2041 ctggtggttt agccatgatg gaagaggcaa agaaaatttt ggagccttat ggcaaagagg
     2101 caccattatt aattgcagtg acggttctaa ccagtatgga agatttggat ttgcttcaaa
     2161 taggtattaa tgcttcgccg atggaacaag ttattcgttt agcacattta gcacaacggg
     2221 ctggtttaga tggcgtggta tgttctccac aagaagttga ggtattacgt acccattgtg
     2281 gtcaagattt taaattagtg accccaggaa tccggccaaa aggaacagat tttggggatc
     2341 aacgccgagt gatgacaccc aaacaagcag tagaaacagg ctcagatttt ttggttattg
     2401 gtcgtcctat tacccaagct caagatccgc ttgcggtatt aaaatctatt aaccaatcta
     2461 tcagttaatt cattgcattt ttaatgaagg cggaaaaatt tccgcctttt ttaatcaaac
     2521 aagcggttat ttttgaggaa aaatttgtaa atgacattag tctattcaac ggaaacaggc
     2581 agaataaagt ccgaagtaat aaaagaagaa cgccctaaag gggatggtat tgtgcgtatt
     2641 caacgtcaaa caagcggtag aaaggggaaa ggtgtttgcc ttatcaccgg tcttgattta
     2701 gaggataaag ccttaaattc tcttgcttca gagcttaaac gtaaattggg ttgtggtggc
     2761 tcatttaaag atggagtaat tgaaatccag ggcgataatc gaggattcat taagcaaatc
     2821 ttagaacaga aaggtttcaa agtgaaactg gcaggtggtt gagttttaaa caattatcag
     2881 ctggcagttt ttaaataact tcattttatt tgaaacttca ttcctacttt tctgcctaaa
     2941 actcacgatt aaggagaaaa actaatgtgg aagaaaacgt tgattgctgt atttttagca
     3001 agccaatatg cttttgcggt aggaaccggg aaagaaccat caaaaagtta tactgaaaca
     3061 gaattaaaac aattaaaaaa tgattggtta gcgatttatc aaaaagagct acagtatgca
     3121 gaaaatttgc gtttaaaaca gagagaaaat tttcagcaaa ttaattattt ggcagatgca
     3181 gcgattagtc agaagaattt atcactttca acggagcaga taattcagcg tttattacta
     3241 accttagcgg gttatccgtt ggagatgaaa gctgaatgga agctgctaaa tgctaaaata
     3301 acgttaaaac aagccacaga aagtgaagtt aaagcattta tttcaaaata tcctgattct
     3361 ttgtatcaaa ggcagttaca gaacttgatg tttgagctgc tctatcgtga acagaaattt
     3421 ccggagttac tggaatatgc tcaaaatgta aaacagagta gtcttgccga ccaatgccgt
     3481 ttattttctg ctcgttatga actggcagca agtaaagctc aaatcaatcc ggaagtttct
     3541 caagtaactg agcaaaaagc agaaaatgca gaactgcttg ctttggtgca agagtttgat
     3601 cgcttttggc ttgctacgcc gaaattaact tctgattgtg cgaatttaga aagttattgg
     3661 cgggatcagg ggcttaaaac cgatgaaaaa atgcgtttga aagcggtcga attaattaaa
     3721 caaaatgcaa atgcagaatt ggcgaatttg gcggcaaatg taaataatga gaatttgaaa
     3781 atctggctat cttccgttga aaaggtagcg aataatccgg cagatttgca aaattttatc
     3841 gaaaatcaac cgcttgattc cccattttat gctgataata aagcgattat tcagcaactc
     3901 tttgctaaat atgtgcgaac tttatcggaa aatatggaga acccgagttt ccagcagtat
     3961 caaacgtggg cagagaagta tcaactttcc agcgaggaaa tcaatggctg gaaaaatgct
     4021 tttatcactc gattttttga taacccagaa ccaacattcc aattttggcg tgatgagcaa
     4081 atcaagttgc tcaagacaga caatttaact gaacgccgtt tacgaatggc aatttggcaa
     4141 aaagcagatt taaatgagtg gctggttctg ctatctcagc aggcgaaaga aaaagcagaa
     4201 tggcgttatt gggctgcgaa agaggagaaa aacgaagcga agcgtaaagt tgattttgaa
     4261 agcttagcta aagagagagg cttttatgct atgttggctg ctcaacaatt aggcattgtt
     4321 taccaaatcc cgatgcttga agtgcctgaa ttgacacaag cacagcttga attgtatgca
     4381 aaccaattag ctcgtattca agagttgcga gagctagggc agtttgacca ggcaagaaaa
     4441 atatggattg actggataaa aaacatcaag ccggaagcag gtgaaaatac accaactaaa
     4501 atccagttag ccctgataaa atatgccaag gataaaaatt ggtatgattt ggcggtggaa
     4561 ggtacaattc aaacaaaagc ctttgattat cttgatttgc gattaccgaa tgcctattcg
     4621 gattggttcg atttacatct gcaagataaa aaaatcagca aaacgtttgc tcaggcgatt
     4681 gctcgtcaag aaagtgcgtg gaatgctcag gctcaatcgc acgctaatgc gagagggcta
     4741 atgcaactgt taccgacaac agcacaaaaa accgctaaag ataacggttt aaccttcaaa
     4801 gatggcaatg atctctttca accgtttaac aatattatgt taggcacaac ccatttaatg
     4861 gagttaaacg aaaaatatcc gaataatcgg attctgattg cctctgctta taatgcagga
     4921 gcagggcgtg ttgagcagtg gctgaaacgt agtaacgggc agttagcaat ggatgaattt
     4981 attgccagta tcccgtttta tgaaaccaga ggctatgtac aaaatgtgct ggcgtatgac
     5041 tactattatc aaatgctcta tagcgatata aatgataaaa atggattaaa aatgttctat
     5101 caagaggaat taacaagaaa atattaaaaa atgcttgtgt ttttgtgtag aaccgctata
     5161 atgcacccca tcagacgcgg ggtggagcag cttggtagct cgtcgggctc ataacccgaa
     5221 ggtcgttggt tcaaatccgg cccccgcaac cagttttaag ctcacagctt tggttaaagt
     5281 tgtgagtttt gttttatatg ctgtagcaaa tttgcaaaaa atttggcaaa aaagaccgct
     5341 tgttgtggtt gctacagcaa aaaacaaacc gcttaaagcc ccacggtttc ccgtctgggg
     5401 cttttttgcg gaaattggta cagcattaac gtatcaaagc tggtttatcc ggcttttttt
     5461 atatttggag attatgactt ggcaacttta gaacaaaaat tagaagattt agtgctcgat
     5521 tctattgaag cattaggctt tgaattagtc ggcattgaat gccaacgtgc aggccgtttt
     5581 ttaacggtgc gtttatatat tgataaagaa ggtggcgtga cgattgatga ttgtagcgat
     5641 gtcagccgcc aagtgagtgc gatttttgat gtggaagatc cgattgccga taaatataac
     5701 ttagaggttt cttcaccagg gttagatcgt ccattattta ccgttgccca ctatgagcgt
     5761 tttatcgggc aagatgtagt gattcattta cgcattccga tgttcgatcg ccgtaaatgg
     5821 caaggtaaaa tcaaacaagt agaaggtgat ttcatcactt taacggtaga aaatgaagat
     5881 cgccagttcg catttggcaa tattcaaaaa gcaaatttag ttccggtttt taatttttaa
     5941 gataaaggta aactgacaaa atgagtaaag agattttatt agctgcggaa gcagtttcaa
     6001 acgaaaagca attaccaaaa gaggcaattt ttgaagcctt agaaaccgct ttaaccattg
     6061 caaccaaaaa gagccgtgat ttagatattg acgtgcgtgt ggtggtagat cgtaaaaccg
     6121 gtgatttcca aactttccgt cgttgggctg tggttgaaaa tgtgaataat atgacccgtg
     6181 aaatcagctt agaagcggca caatttgaag atccaaacgt acaattaggc gattttattg
     6241 aagatgaagt agagtctatc gagttcggtc gtgtggcgat tcagactttt ggtcaggtag
     6301 tcaaaaccaa aattcgtgaa gcggagaaga cgaaaatcat tgatgaattc cgttcggatt
     6361 taggcaaaat tattcttgcc actgtgaaaa aagtaacccg tgataatatt ttccttgagt
     6421 tacaaggtaa atcagaagat atcaaaggcg aagcggtcat tgtgcgtgaa gatatgatcc
     6481 cacgtgaaaa cttccgcccg ggtgaccgtg tgcgtggtgt gttatacgaa atcaagcctg
     6541 aatcaaaagg acctcagctt ttcgttaccc gtgctaaacc ggtgatgtta gaagagctat
     6601 tcaaattgga agtgcctgaa attggcgaag gcgtaattga gatcaaaggt tcagcccgtg
     6661 atgtcggctc tcgtgcaaaa attgccgtga aaacccacga taaacgtatt gacccggtag
     6721 gggcttgtgt gggtatgcgt ggtgctcgtg ttcaagctat cagcaatgaa ttaggcggcg
     6781 agcgtgtgga tatcgtactt tgggacgaaa atccggctca atttgtgatt aatgcaatgg
     6841 ctccggcaga tgtaagctct attgtgatta acgatgaaac tcactcaatg gatattgcgg
     6901 ttgaagcgaa aaatctggct caagctatcg gtcgtaacgg tcaaaacgtg cgtttggcga
     6961 cacaattaac tggttgggca ttaaatgtaa tgaccgttga agagctaacg aagaagagcg
     7021 aagaagaaga tggtaaagtc atcaatttat taacttctgc tttagaaatt gatgaagaat
     7081 ttgctcaagt tttaattgaa gagggcttta cgtctcttga agcgattgct tatgtgccgg
     7141 ttgatacttt aacggcgatt gacggtttag aagatgaaga tttagtcgaa gaattacaat
     7201 ctcgtgccaa aaatgcgatc acgaaaaaag cgttagaaga agagaagtta ttaaaatcag
     7261 ctcaaattga agaaaaatta ttaacgcttg aaggtatgga tcgccatatc gcattcaaat
     7321 tagctgaaaa acagattacg actcttgaag aacttgcaga gcaaggtgtt gatgatttag
     7381 ccgatattga agaactttca gaagagaaag ctggcgagct aattatggca gcacgccaaa
     7441 tttgttggtt caactaagca aagaggataa taaaatgaca gaagagaaaa aaacactttc
     7501 gcttggtggc gctcgcaaag cgagtaaagt gaatacaacc acaaccagtg gtaaagtaaa
     7561 agcggttgaa gtaaaagaga aaaaaccgaa aattgatgca aaagcattgc aagaaaaagc
     7621 agcaaaagaa gctgccgcag cgaaagctag agctgaaaaa gaagcagctg aaaagttagc
     7681 caaagaaaaa gccgaagctg aagctaaaga gaaagctgca aaaaaagcag cagaatcgac
     7741 cgctagtaaa ccgatttctg tgccggtaat gccaagcagc aaagcaaaag aagaaaaacc
     7801 gaaagcacaa caggtacagc caaaacaaga gaaagcggtt gatcctgaaa aagaagctaa
     7861 acgcaaagaa gaagctgagt tacgccgtaa gcaagaggaa ttagctcagc aaaaagcgga
     7921 aatggaggca aaacgtgctg cagaaaatgc ccgccgttta gcagaaattg ctcgtgaaga
     7981 agcggaagat aacggcaacg aagattttga tgatgagcgt tttacttctt cttatgctag
     8041 agaggctgag cgtgataatg accgccgtag tgagggaaat cgtggtcgta acggcaaagg
     8101 caatgtggct aaagcgaaaa aaggcggacg tgaagaggat aaaaacgaac gcagtgcaga
     8161 tcgtcgcaac cataaagaag taaaaggcaa aggtaaacaa ggtaagaaag gtgcagcttt
     8221 acaacaagcc tttaccaaac cagctcaagt tgttaaagct gatgtggtga ttggtgaaac
     8281 tatcactgtg gctgaattgg ctaataaaat ggcagtgaaa gcgactgaaa tcatcaaaat
     8341 gatgatgaaa atgggtgaaa tggtgacgat caaccaagtt atcgaccaag aaaccgcaca
     8401 attagtggcg gaagaacttg ggcataaagt gattttgcgt aaagaaaatg agttagaaga
     8461 agcggtttta gaagatcgtg atactcacgc agaaaaagta acccgtgcac cggtagtgac
     8521 tattatgggc cacgttgacc acggtaaaac ctcgttactt gactacattc gtaaagccaa
     8581 agtggcggca ggtgaagcag gtggtattac ccagcacatt ggtgcatacc acgttgaaac
     8641 cgatgacggc aagatgatca ctttcttaga taccccgggg catgcagcct ttacctcaat
     8701 gcgtgcgcgt ggtgcgaaag caactgacat cgttgttctc gtcgttgcgg ctgatgacgg
     8761 tgtaatgcca caaactattg aagctatcca gcacgccaaa gcagcgggtg cgccattagt
     8821 ggtagcggtg aataaaattg ataaaccgga agcgaaccct gaccgtgtag aacaagagtt
     8881 actacaacac gaggttattt ctgagaaatt cggtggcgat gtgcaattcg tgccggtatc
     8941 tgcgaaaaaa ggtatgggga tcgatgattt actagaagcc attattctgc aatccgaagt
     9001 gcttgaatta accgcagtaa aagacggtat ggcaagcggc gtggtaatcg aatcttactt
     9061 agataaaggt cgtggccctg tggcaactat cttggttcaa tccggtacgc taaacaaagg
     9121 cgacattgtt ctatgtggtt ttgaatacgg acgtgtgcgt gcaatgcgtg atgaaaacgg
     9181 taaagatgtt gaatcagcag gtccgtctat tccggtagaa gtattaggct tatcaggcgt
     9241 gcctgctgcc ggtgatgagg caaccgtcgt gcgtgatgag aagaaagccc gagaagtggc
     9301 tctttatcgc caaggtaaat tccgtgaagt aaaactggct cgtcagcaaa aagctaaact
     9361 ggaaaatatg ttcagtaata tggcagccgg tgatgtggca gaattgaaca tcattgttaa
     9421 agcagacgta caaggctcgg tagaagcgat cagccaatcc ttaatggaac tttcaaccga
     9481 tgaggtgaaa gtgaaaattg ttggttcagg tgtaggtggt attaccgaaa ctgatgcaac
     9541 cttagccgca gcttctaatg ctattatggt cggctttaac gtgcgtgccg atgcctctgc
     9601 ccgccgtgtg attgaaaacg aaaatatcga tttacgttac tactccatca tttatgagct
     9661 attaaatgac gtgaaagcag caatgagcgg tatgttacag cctgaattca aacaagaaat
     9721 tatcggcttg gcagaagtgc gcgatgtgtt ccgccacccg aaatttgggg cgattgccgg
     9781 ttgtatggtg acagaaggta tcgttaaacg taacaaccca atccgtgttc tgcgtgataa
     9841 cgtagtaatt tttgaaggcg agttagaatc tctccgccgt ttcaaagatg acgtatctga
     9901 ggtgcgtaac ggtatggaat gtggtatcgg cgttaaaaac tacaacgacg taaaagttgg
     9961 cgaccaaatc gaagtgtttg aagtggttga agtaaaacgt actatttagc ctaattttgt
    10021 gtaggggcgt attgcatacg ccctttgtat tttttagggc gtgaagcatc acgcccctat
    10081 aaaagagaaa aatatgtcca gagaatttaa acgtagtgac cgtgtggcac aggagctaca
    10141 aaaagaagtg gcggtgattt tacaacgtga ggtaaaagat cctcgcattg gtatggtgac
    10201 tgtttctgat gtggaagtat caagagattt ggcgtatgcc aaaatttttg tgactttctt
    10261 atttgataac gatgctgaag ctattaagca aggtatgcaa ggcttggaga aagcaagccc
    10321 ctatattcga acccttttag gtaaagcaat gcgtttgcgt attgtgcctg aactgcgttt
    10381 tatctacgat gaatcgctag tcgaaggtat gcgtatgtct aaccttgtgt ctagtgtaat
    10441 caaaaatgac gaagaaaaac ataaagagga ctaattgtgt ctagacctcg caaacgtggc
    10501 cgtgatattc acggcgtttt cttattagat aaaccacaag gaatgtcttc aaacgatatt
    10561 ctgcaaaagg taaagcgaat tttccaagca aataaagccg gacataccgg ggcgttagac
    10621 cctctcgcaa ccggaatgct gccgatctgt ttaggtgaag cgaccaaatt ttcgcaattt
    10681 ttgttggatt cggataaacg ttatctcgtc accgcaaaat taggcgagcg aaccgatact
    10741 tcagatgcgg aagggcaagt ggtggaaacc aaagcagttg cagtgacaaa atctcaaatt
    10801 ttggctgctt tggagaaatt tagaggtgat attttgcaag tgccgacaat gttttcggcc
    10861 ttaaaacaca acggcaaacc cttgtatgaa tatgcccgag cagggattac ggtggagcgt
    10921 gaggctcgcc cgattacgat ttttgaacta aatttcattg aatacaaggc tccattttta
    10981 acccttgagg tgcattgttc taaaggtact tatatccgaa ctttggtcga tgatttaggc
    11041 gaagagttgg gctgtggtgc acacgttacg atgctacgcc gtacagcagt ggcagattat
    11101 ccgattgaaa aaatgatgag ctatgcagat ttgcaaaatt tagccgaaaa tcaaccgctt
    11161 gcagagttag accagcactt attgccgatg gatacggcag tttctgcatt agcaaaaata
    11221 aatttaagtt cagagcaaac taaagcggtg ggttttggtc agagaattag atttgataat
    11281 ctgaaccaac tttatggttt ggtacgttta tattcagaag atgaacaatt tctcggtgtg
    11341 gcagaaatca ccaatgatac tgtgattcgt cctaaccgaa tgattaactt aacggaataa
    11401 gaatgaaatc gataattttt accttacctt tagcgattat cactttagcc ttagccggtt
    11461 gttcaagtga taatcaagaa agttatgccg gcttaaactt agatgctaaa tacagtaaat
    11521 ctcgctcggt cagtcatttt aatgattatg tgcattttct gaaacagaag gcagcagggg
    11581 caggagtgtc tgcaaaaacc ttaaatgctc agaaattcat tcattacaac gccagagcgg
    11641 tgcagttaga tcaacagcaa gctgcacgaa gacgtgaccc gaatgtaccg cctccgccac
    11701 caaatccaag ttttaaacaa agtattaact caagcgaagg tggatcaggc aagtgaacgt
    11761 tggtatgaat ataatagcca gcttacccat gccagccaaa aatatggggt acaaaaagag
    11821 tatattttgg cactttgggg aatggagagc agctttgggc gtaaccaagg ggattttgat
    11881 gtactttcgg tgttggcaac cctcgccttt gatggtcgcc gtgagaagtt atttacgcaa
    11941 gaatttgtga atgcgatgaa aatgctcgat gacggcacaa ttaaacgcta tgaattgctt
    12001 ggatcttggg caggagcgat gggacaaact caatttatgc caacttctta cttaacctat
    12061 gcagctgatg gcaataatga cggtaaaaaa gatatttggg agaatgaagc cgatgcattt
    12121 gcctcgattg ccaattatct ttcaacagta ggttgggata atcaactacc ttggggtgtt
    12181 gaggttaaat tagcttcacc gatggattta agtttcgccg gtcttgaaac caacaaagcg
    12241 aaaacattgg cggaatggca agccttaggt atttatttag cttatccgaa tggacaagaa
    12301 tcggcaaaat tagctcaatt acacggcaaa gaggtttggt tgattcgtcc ggataaagaa
    12361 gcgggaagag cgtttttagt ttctaataat ttccgtactt taatggattg gaataaatcg
    12421 aataaccttg gtatcagtat cggaaaattt gccgatcgaa ttttagcttc agttggtcaa
    12481 taatcgaata aaagcatagt agcaaagcct atctatgctt ttattttatc aaagcgtcgc
    12541 ctttcttctg caaacgacgt tgcttaaaaa aatcactgat cttttggcta cattctctgc
    12601 ccaatacatt gccatgcact tctaagaaat ggttcatttt ataatcttca aacagatgat
    12661 agcgtgaacc taccgcacct gttttataat cacttgcccc aaaaaccaat cgcttaatcc
    12721 gactatgtaa aattgcaccg gcacacattg ggcaaggctc gagcgtaaca taaagggtac
    12781 aatctaacag acggtaattc cctaaagttt gtcctgcttg acgaattgcc tgcatttcag
    12841 cgtgtgcaga tggatcacag agttgaatgg tttggttcca gcctctgccg ataatctgtt
    12901 ggtttttatc aaccagtact gccccaaccg ggatttcgcc ttttgcttcg gctaaatcag
    12961 cgagatcaag ggcgtattgc ataaaggcga tgtcttgctc ggagatctga agtggacaag
    13021 aagtttgatt tggtttgata aacatagttg caaaaaattg agtttttttg accgcttgta
    13081 aaatgcccca cgagggtggg gcatcgctta cttcgcttag tgtcgtttag tcacgacgac
    13141 cacgtttttc actgaaacgt ttttctttaa agccaccttt gcggtcttta ttgaaacccc
    13201 cgtctcggcg accaccacga tcattacgat cgttaccacg gcgaccacga ccttcaaatg
    13261 gatttgcatt acttgcactg tttgcagagc caagtaatgt catctgcatt ggtttgctta
    13321 atacacgtgc tttgtgagcg aaatgttgta cgagatgatt tggcattcct tgcggcaact
    13381 caatggttga gtatttatca taaagtttga tatgcccgat gtaacggctg ctgatatccc
    13441 cctcgttggc aatcgctcca acaatatgac gaacttccac accgtcttca cgccccaatt
    13501 caatgcggta taaatccatt gccacaccat tgttttcacg gtgttcacga ccgttacgac
    13561 gatcgtttcg ctcattgcgg tcacgtccac gttcagaacg ggcagcacgc acaaccggat
    13621 ccggtggcag aatcagtttt tgtttttctt gcagtaacat catcatcgcc gctgctaatt
    13681 cttcgtggtc ttgatccgca gtaaataaat cttccaacaa ttcacgatat ttttctaaat
    13741 cgtgatgttc taactgctta gaaatgcggg ctttgaattt ctcacggcgt ttttccatta
    13801 aaatttcgtg gtttggaatc gccacttcat taattggttt tttgattaaa ttttcgatat
    13861 tgcgtaataa gcggcgttca cgaggctcaa caaacagtaa ggcacgacct gaacgaccgg
    13921 cacgaccggt acgtccgatg cggtgaacat aagattcttc ttctaacgga atatcgtagt
    13981 taataaccaa gctgattcgc tcaatatcaa taccacgggc agcaacatcg gtggcgacta
    14041 aaatatctaa acgaccggag cgtaatttat ctaaggtttg ctcacgggct tgctgggtca
    14101 tatcgccgtt taatgccgca gtacggtagc cgttgcgttc acacaattcg gtaatatcaa
    14161 tagtaccggt tttggtgcgg gtaaagatga tagcagcatc aaattcttct acttctagga
    14221 aacgcaataa cgcatcgttt ttacggaagc cgtttaccag ccagtagctt tgttcaatat
    14281 ccggggctga acgctcggtt gccttaattt ttacctcttg cggatctttc ataaaacgac
    14341 gggtaatacg gcgaatcggc tccggcatgg tggcagagaa gagagcagtt tgatgtgttt
    14401 ccggtaattc agccattacc gtttcaacat cgtcaataaa gcccatacgc agcatttcat
    14461 cggcttcatc taacacgatg aattttaatt cggataaatc taatgtgcca cggcggatat
    14521 ggtcaagaat acgcccaggt gtacccacta ctacttgtga gccttgtttt aaagcacgta
    14581 attgaatgtc gtaacgttgt ccaccataaa ccgtcactac acgaatgcct ttcatatttt
    14641 ttgcaaactg ctcacaagca tcggctactt gaatcgcaag ctcacgggtg ggtgccatca
    14701 ccaacatttg cgggtggcgt tgtgttgggt caatttgtgc caacagcggc agcgagaatg
    14761 cggcggtttt accgctcccg gtttgtgcca taccaagcac atcacgaccg gctaataaat
    14821 gaggaataca ttcttgttga atgggtgatg ggtttacaaa gcccatttca tttacagcat
    14881 caaggattgg ttgaggtaag cctaaatcgg cgaaagttaa agattcagtt gtcataaaaa
    14941 accatttgtt tggaacgcac aaagcagaaa atatcgacta tttttctgca cagccattaa
    15001 tgaaaattat attgttgaaa tttaggaaga aatgaaataa caaagcaagt gatgctttaa
    15061 aaatcccctg agtataagcg gttaaattta caaaattttt tgcgaaatta accgcttgta
    15121 ttgtcctaat ctttccctat tctttatcaa caacgggagt cgtttgagtc ataccacgaa
    15181 tttgtgataa ctcaaacagg gcaaaacgat actcaacgaa gttaaacact tgatttgcaa
    15241 gagcaagacg aaagaggctg attgcctcat ctttatgatc taatttgagt tgttgttttg
    15301 ctaagtagaa atacgtttct gtcagtattt ctgcatattg tgagctattt tccttagcgt
    15361 cacgttccat tatagcacga acttgagcta aattgagctt ccctaaaaaa taatttacga
    15421 tatttgttcc ccagtaatcc gaagaaagat tattagctcg cttttgtaaa ttatttttag
    15481 cttctgttgg tgtaaattcc agctcattaa aatagagcca taatgctcga taaggatctt
    15541 tcttatcttc ttcataaaaa tggagaagat cacgctgggc ttcagaatat ctgccgctat
    15601 aatagaaagc caaacctcga ttgagatagg tgtagctata ttcccgatct aattccaata
    15661 cggcattaaa cgcatcaatc gaactgtcgt aatcgccttc cagcagcaga tacagcccca
    15721 tatagttata agctgccgcc atacgttgat tgagcgaaat ggcttgagca aaatcataac
    15781 gggctaacga ccataaacct aaactgtcgt aaataacgcc tctttcaaaa tagaggtctg
    15841 cttgctcaga gggcgttaat ggggcttctt ccaatacttg gctgagtctt accaccattg
    15901 cctcttgttc aaagcgaagt tgaggattaa gttctgccaa tgctaagttt ttaattgaaa
    15961 ccaaatcggc ataccgactg gtacagccgc ttagcattaa aacagaaatt aaattcagta
    16021 ataagaaacg gattcgaaac aacttaaaaa gttgtaccat tacagcatat ccctcaatta
    16081 aggaaaatta aaaactgaat aacggacact caaagtccgt tattcgcctt tattattgaa
    16141 ctagaaatta ttttcttgtt ctgtttctac cacattttca gctacagttt cttctgcatt
    16201 tgcctcgttg gtattattga tgtctttcat cgtcagacga atgcgtcctt gacggtcaat
    16261 ttctaccact ttcacttgta cttcttgacc aacggttaag tagtccgcta cacgctcaac
    16321 acgttcgttg gtgatttgtg aaatgtggac taaaccttct ttgccaccta agatggaaac
    16381 gaatgcaccg aagtccacca cacgggttac tttaccgttg tagatttggt ttacttccac
    16441 ttcggcaacg atctcttcaa tacgagccat tactgctttc gctgcattgc cgtcagttgc
    16501 cgcaattttc accgtaccgt catcatcaat gtcgatagaa gtattggtct cttcggttaa
    16561 agcacgaatt gttgcaccgc ctttaccaat cacatctttg attttcttcg gatcgatctt
    16621 cattgtgtga atgcgtgggg catagtcaga aatatctgca cgaggtgccg gaatggcttg
    16681 ttccattaca cctaaaatgt gcattcttgc acctttagct tggtttaagg caatacgcat
    16741 aatttcaggg gtgatccctt cgattttgat gtccatttgc agcgcagtca caccggtacg
    16801 ggttcccgct actttaaagt ccatatcgcc taagtggtct tcatcaccta agatgtcaga
    16861 aagtaccacg aatttctcgt cttctttcac gagccccatt gcgataccgg caaccgctgc
    16921 tttgattggc acacctgcgt ccatcaacgc aagagacgca ccacacacag atgccattga
    16981 agaagaaccg ttagattcag tgatttcaga caccacacgc actacatacg ggaactcttc
    17041 agcggttggc atcacggcta atacgccacg ttttgctaaa cgaccgtgac cgatttcacg
    17101 gcgttttggc gagccgatac gaccggtttc gcccacagag tatggaggga aattgtagtg
    17161 gaataagaaa cggtcagatt tttcgccggt caattcgtca atgatttggg catcacgctc
    17221 tgtgcccaat gttgctactg ctaatgcttg ggtttcgcca cgggtgaaaa gagcagaacc
    17281 gtgggtgcgt ggtaacacac tggtgcaaat atccaatgca cgcacggtat ccaccgtacg
    17341 gccgtcaatg cgtggttcgc ctgcaataat acggctacgc acgatttggc tctctaatgc
    17401 ggtgatgatg tcgatgatag tcccttcaga aacagtttca tcttctgcgg taagttgtgc
    17461 aatcacctct gctttaatcg catcgatttg ctcgtaacga acttgttttt ctacgatacg
    17521 ataagcatcg ccaaggcgtg tttctgctaa tgcttttact ttgttgatta aatctgtatt
    17581 tggctctggt gcaacccaat cccaacgtgg tttgcccgct tctttaacaa attctttgat
    17641 gttttcgatt acaacctgtt gttgctcgtg gccgaacacc accgccgcta acatttgctc
    17701 ttcggttaag atatccgctt cagattccac catcaacacg gctttgtctg tacctgaaac
    17761 cactaaatct aagcggctga ttttttgctc gctggtggtt gggtttaata cgaattgatc
    17821 gttgataaaa ccgacacgag ccgcaccgat tggaccgtta aacggcacgc cggataatga
    17881 aagggcagcc gatgcaccga tcatcgcaac cagatcagga ctgatttgtg ggtttaccga
    17941 tactacggtc gcaatcactt gaatttcgtt aaagaaacct tctgggaaaa gtggacgcac
    18001 aggacggtcg atcaagcgag cgattaaggt ttcaccttcg ctaggacgtc cttcacgttt
    18061 gaagaaaccg cccggaatac gaccggctgc gtaagtacgc tcttgataat ctacggttaa
    18121 tgggaagaaa tcttgccctt cttttacgtc ttttttcgct actacggtaa caaatacggt
    18181 tgtgtcgtcc atacttgcca ttactgctgc cgttgcttgg cgtgcgatag caccggtttc
    18241 taaggtcacg gtgtgctgac cgtatttaaa ctgttttaca attggagtca ttgaaatttt
    18301 ccttttaaag aatttactat tattgtcgtt ttatttctct aaattgcgac tagcaaaaag
    18361 gcttttaagt aaaacatttt tgatagccgc ctctcaataa caagaaataa aacttgtcta
    18421 gtatagcgtg atttttataa aaaatcgcct attattttga tcttgcacct cagttataaa
    18481 gaaattcaaa acttcagtgt aaaatgggga ttagtttgtg aaaagccttg tgaaaatagt
    18541 ttgattgcat cgctattttt ttattagagg tagtttaaaa ctaaatctgc ttcctttacc
    18601 ggcttcactt tcaatttcca gtgtggaatg gtgctgttca agagcgtgtt tgacaatagc
    18661 cagacctaaa ccgctaccgc cggttttgtt gctacgagat tcgtccacac gatagaaacg
    18721 ttctgtcaga tgggtaatat gatgggcatc gataccaata ccgtcatctt gcacgctgaa
    18781 aatcgcagta ttcggctctt gattatcacg ttgacaacat atttgaatgg taactccttc
    18841 accggcgtgc cgtattgcat tgtaaattaa atttgataca gcgctttgga gttgctcttg
    18901 atctccccat accgttaaat taggctcaat atcaaacagt atttgctgat tttggcttaa
    18961 aatctcggta tttttttgta tggcggagat taacgccgac atctggacct cattgtgttc
    19021 ggaatcattg gaatgctcga ttttaaccaa aagtgagagt tggcgaagca ggttagtcat
    19081 acgttgactt tgctctttca tcgcattgag cgctttttgc tgtaatgcca tattatgcgg
    19141 ctcgttttcc agcatttcca aataaccttg taatatggta agaggtgttc gcagttcgtg
    19201 gttcatattt gccaaaaagg tttgtcgtga gtgcataagg cggacaaaag aggttacatc
    19261 tcgtgcgaca ataagtctgg agttactgtc atatcgattg agattcaatt caatatagcg
    19321 tttattatga gtcagcagta ccaatgggcg atggtgtcgt tgttggcgga aatagctttt
    19381 aaattcagga taaaaaatca cattaaaaat gcttttattt gattttttgt cccaataaaa
    19441 ctcaaatagc tcttgtgcaa cattattgca ccaagaaatc tccccgtttt cttggcaaat
    19501 aatgatcgca tcgggtaagt attgaatatg cttatttaat tttgataaca gacgtaaggt
    19561 tttgatttta tcttggcgta aacgttcgtg atagaatgcg gcactttgag ataaatgctc
    19621 cattgtcgtt acttttgacc tgccgctttg ctcattaggt ctgagtaact gaaggagaga
    19681 agattcggtg tggtgatgcc aaataagtag aaccagtaaa caaaaactaa accagaaaaa
    19741 ataagcaggg aaaaacagat tgaatacgcc ggcaacggct gctgccagca aaagttccaa
    19801 taaaaagtgt ttaagtgaga gcttaacttt cattattagt cctgaaaatg gttggaaaaa
    19861 cggtatcccg agccacgtac ggtttgaata taacgctcaa aaccataggg ttcaagactc
    19921 ttacgcaatc gccgaatata agagtctacc gtgcgatcct caacataaat atcattaccc
    19981 caaataaaat caagcaactg ttcacgacga tacacttttt cagggtgagt cattaaaaag
    20041 tgaagcagtt tatattccgt gccgcttaat ttcacttcct gttcggcaac caaaacccgt
    20101 tgtgcctgtt gatcaagaat caatccgtct atattgatga gattgcttgg cggatcatag
    20161 cttcgccgcc atactgcttc aatccgggca agcaacacct gtggtgaaaa cggtttggta
    20221 atgtagtcgt ctgcaccggc attcagaccg gtaatacagt cttcttcgct actgcgggct
    20281 gttagcataa tgattggaat tttttcggtt tcaggctgtt tttttaggta tttaattaaa
    20341 tggatacccg aacgccccgg catcatccaa tccagtaaaa taagctcggg tttttcgccc
    20401 agtttattga tagcactttg gtagtcttcc gcttcaatta cctgatattg tttgctgaga
    20461 tagagactta gcatttcacg aatagcacgt tcgtcttcaa cgatcagaat tttgttattc
    20521 attcttcctc tccgatgcgg tgtgacaagc ggtcgattta tcgcagaaat ttgcaaattt
    20581 tttactaaaa ccgaccgctt gcagattacc ccattttacc tttaatgtaa tcttccgtct
    20641 gccggaattt tggtttgtcg aaaatttgtt tggtatcacc gaattcaatc agctcgccta
    20701 gatacatata agcggtatag tcagaacagc gggctgcttg ctgcatattg tgggtaacta
    20761 tcgctaccgt gtagtcgtgc ttgagttcgt tgataagttc ttcgattttc atagtcgaaa
    20821 tcgggtcaag tgcggagcag ggctcatcca gcagtaacac ctcgggtttc actgcaatac
    20881 ctcgtgcaat acagagccgt tgctgttgcc cgccggagag actgtcacca ttttggttta
    20941 atttatgctt tacctcattc cataaagccg ctttggttaa tgcccactct acacgatcgt
    21001 ttagctcgga tttcggcaat ttttcaaaca gacgaatacc aaaagcaaca ttatcgtaaa
    21061 tggacatcgg gaatggggtc ggtttttgga aaaccatacc gactttggct cgaataatcg
    21121 aaatatccgt atcggaggtg agtagattct ctccatcaag attgatttcc cccgttgcac
    21181 gttgattcgg gtagagctca aacattcggt ttagggttcg cagtaatgtt gattttccac
    21241 aaccggaagg accgataaat gcggtcactt tatttttggc aatacgcata ttgatatttt
    21301 tcagagcgtg gaaatcgcca taataaaaat tgaggttgtt aatatgtaat tttgtctcta
    21361 aatccgttgg taacatatag agtccttatt ttgttttgct gaaatacaga cgagtgatga
    21421 tattaagaga aagcacaaac agagtaataa gcattgcacc tgcccaagcc aatgtattcc
    21481 agtcagcgaa cgggctggct gcgtattgat aaatcaccac agggaggttt gccattggtg
    21541 cattcatatc taaagaggca aactggttgg ataatgcggt aaacaatagc ggtgcggttt
    21601 ctccggaaat acgggcaaca gcgagcaaca cgccggtaat aatgccggag cgggcggcac
    21661 gataagagat cattgtaatt accctccatc gtgggcagcc gagagccgca gctgcttcac
    21721 gcagattatt aggcacaaga ttgagcatac tgtcggtagt tcgaacaaca atcggaatca
    21781 ctaataccgc caatgctaat gagcctgccc agcctgaata gtgtttaacc tgtgcgacat
    21841 aaatcgcata aataaataag ccgataataa tagaaggagc agagagcaac acatcattca
    21901 aaaaacgggt gaattttgcc aatttggtat aacgcccgta ttcgctcaag taagtgccgg
    21961 ctaaaatacc gattggtgtt gcaatcagtg ttgcggtact gacaatcagt atagagccga
    22021 caattgcatt acgtaatcct cctgtttcat ttggtgcagg agtcgggcgg gtaaacagtt
    22081 cccaagacag tgcagggata cctttttgga gtaaagtaac aataatccag accagccaaa
    22141 ataagccaaa taatacagcg gaataggcaa gacttaacat caagacattt tttatttttc
    22201 ggcaataaaa gcgtcggttt tggtaatttt tcattctcgt tttccttcct gtttacccat
    22261 tcgtgcgatt aataaacgtg aagcggctaa aacaatcgaa gtaataagga acagaattag
    22321 ccctaggctc atcaacgctg atttttgtaa tccgccggct tcattaaatt cattggcgat
    22381 agcagaagcg atggaggttg aaggggaaaa taccgaatca ggtagcataa aggcattacc
    22441 gatcacaaag gtaactgcca ttgtttcgcc taatgctcga cctaacccga gcataattgc
    22501 accgattact ccggttttgg tgtaaggtag cacgactttc caaataactt cccaagtggt
    22561 tgagcctaaa ccgtaagcag actctttcag taaaggcggt acaacactga aaacatcacg
    22621 cataattgag gcaataaagg gaataatcat aatcgccaat accaagccgg cactgaatag
    22681 cccgatcccg aaaggagctc cgctaaaaag cataccgata aagggaagct gaccaaatgt
    22741 atctatcaaa tacggttgca catattcttg aaataagggt acaaatacaa acagccccca
    22801 cataccgtaa ataatagagg gaatagccgc tagcatttct accgctgtgc cgacagggcg
    22861 ttttagccat tgcggtgcaa gttcggttaa gaaagtcgca atgccgaacg agaccggaac
    22921 ggcaatgact aatgcaatgc cggcagaaat caatgtgccg ataatcggca ctaatgcacc
    22981 gtatcggtca ttcacagggt tccactcgtt ttcccatagg aatgataccc cgaatcggct
    23041 taatgcttcc caactgccaa gcaataatga aaccagcacc accaccaaca aggcaaaaac
    23101 cagcaaagca aagaaacggg ttgtgtgatg aaataacccc tctacccaag gatggtttaa
    23161 gcatttgtta tcaattttat gtttatgcat agtgacaact cttttgcaaa atgttgtgaa
    23221 aaaacgaccg cttgttgtta gttaagctcc atacaagcgg tcgttttgca ttagtagagc
    23281 gaatttcctt gtccatcttt aacttccgtt ttccattgag tttggatttt ctctactaca
    23341 ttatccggta gaggcacata atccagttcg gcagccgcat ctttaccgtg cttaaatgcc
    23401 cagtcgaaga atttaaatac ccctttagtt tgctctggtt tgtcgctatg ttgatgaatc
    23461 aagataaaac tggcggcggt aatcggccag gctttttctc ccgcttcgtt ggttaaaata
    23521 acgcccatac cggctgtttg gctccaatcg gcgttagcag cggcagcagc aaacgtttca
    23581 cgggaaggct gaacaaattt accatctgca ttttgcatag aaacccaagc cagagcattt
    23641 tgtttagcat aggcatattc tacgtaacca atagaatact tgagctgttt cacataagct
    23701 gcgacacctt catttccttt tccgccttgt ccgaccggcc atttcaccga ttttccttcg
    23761 ccgactttct ccttccattc ggcagagact ttagaaagat agtttgtcca accgaaagtt
    23821 gtgcctgaac cgtctgagcg gcgtacgact aaaattgttt ttgccggcag ggcgatatct
    23881 tgattaagtg cgacaattgc cggatcgttc cattgtgtaa ttttgcctag gaatatatcg
    23941 gcaagaactt taccggacag tttcagttcg cccgatttaa gttcaggaat attgacaacc
    24001 ggtacagttc ccccaatgat ggcgggaaat tgcaaaagtt tgtgttctgc taatgtttcg
    24061 cctttcatcg ggtcatcaga agcaccgaaa tcaattgttt tggcaataat ttgttgctga
    24121 ccgccaccgg aaccgataga ttggtaattg attttattac cggtctcttt ttcatataaa
    24181 cctgcccatt tggcgtagat aggataaggg aaagaagcac cggctcctgt gatggtttga
    24241 gcatttgctg cgacagaaag tgaaaaagaa atgcctaaaa cggtaagtag tttaaatcgt
    24301 ttggcaatca tattgttctc cttaaagtta gcgtgagtta atttcacgtg cttagtgtag
    24361 aaaaagaata tgacaaaatg atgacaatgg taaaaaaatc agaaaaaacg accgcttgtg
    24421 tttcaggcta gactaaacag gctaaatcgt agaaaatagc cctcgttttt ttaatataaa
    24481 tatcgataaa aaatgaccac agaaatcaat tatgaaggca ttgaacagat gccgattaag
    24541 cgttttaccg aagatgctta cctcaattat tcgatgtatg taattatgga ccgggcattg
    24601 ccgtttattg gcgatggctt gaaaccggtg caacgccgta ttatttatgc gatgtcggaa
    24661 cttgggctaa acgcctcggc aaagtataaa aaatcagccc gtaccgtggg ggatgtatta
    24721 ggtaaattcc atccgcacgg tgatagtgcc tgctataaag caatggtgct aatggctcaa
    24781 ccgttctctt accgctatcc gttagtggac ggacaaggca actggggggc tccggacgat
    24841 ccgaaatcct tcgctgcaat gcgttatacc gaatcgaaac tgtctaaaat tgcagaaatt
    24901 ttattgggcg agttagggca aggaacggtg gattatcagc caaattttga tggctcgttg
    24961 gaagaaccga aatatctgcc ggctcgttta ccgcatattt tgttgaatgg cacgatgggg
    25021 attgcggtgg gaatggcaac cgatattcca ccacacaata ttaatgaatt ggctgatgcc
    25081 agtgtgatgt tgctggataa cccgaaagca acgcttgatg atgtactaag cgtggtacaa
    25141 gggccggatt acccgacaga ggctgaaatc atcacaccaa aagcggaaat tgccagaatg
    25201 tatgagcagg ggcgtggttc tattaaaatg cgtgcggtgt ggaaaaaaga agagggtgaa
    25261 attgtgattt cagccttgcc gcaccaagcc tctccttcaa aaattattga gcaagtagca
    25321 acccaaatgc ggaataaaaa actaccgatg gtggacgata ttcgtgatga atctgaccac
    25381 gagaacccga ttcgcattgt gattgtgcca cgctctaatc gcattgattt tgatgcgtta
    25441 atggatcacc tgtttgcgac gaccgatttg gagaaaagct accgagtcaa tatgaatatg
    25501 attggcttag acggcaagcc ggcagtgaag aatttgctga ctattctcaa tgagtggctg
    25561 agttttagac gtaccactgt cactcgccgc ctgaattacc gattagataa aattttaaac
    25621 cgcttgcata ttttagacgg tttgatgatt gcatttctca atattgatga agtgattgag
    25681 attattcgca atgaagatga gccgaaagcc gagttaatgg ctcgttttaa tctgaccgat
    25741 gtgcaagcag aggcgatcct gaacttacgt ttacgccact tagccaaatt ggaagagcat
    25801 caattacagg cggaaaaatc cgaactggaa aaagaacgtg atgaattgca actgatttta
    25861 ggctcggaac gccgtttgaa cagcctgatc aagaaagaga ttcaagccga tgccaaagca
    25921 tttgccagcc cacgccgctc accattggtt gaacgagcgg agtctaaagc gattgcggaa
    25981 agtgacttga ctccaaccga agatgtgact gtgattttgt ctgaaaaagg ctgggtacgt
    26041 tgtgcgaaag gacacgatat tgatgttcaa gccctaagcc atcgagccgg agatggttat
    26101 cttgcccacg caaggggtag aagtaatcag cctgtggtat ttttagacag tacagggcga
    26161 gcttatgctc tcgatccaac ttcactgcct tcggcacgct cgcaaggcga accgcttaca
    26221 ggcaaaatca ccctaccgga aggtgcagta gtgcagcaag tgctaatggc aagtccagaa
    26281 actaaagtgc taatggcatc cgattcaggt tacggcttta tttgcacctt tgcagattta
    26341 gtttctcgta ataaagccgg caaagcggtt atttctttaa ccgaaaatgc aaaagtactg
    26401 ccgccgcaat tattggaaaa tgatgaaaat ttatcgcttg tagcgatgag taatgttggc
    26461 agaatgttgg ttttcccggt gagtgaatta cctcaacttt ccaaaggtaa aggcaataaa
    26521 atcatcaata tttccgccgc agcggcaaaa tccggtgatg aatatttagc ccgtttatta
    26581 gtcatcaaac caaccaactc gctggtcttt gtgtccggta aacgcaaaat cacattaaaa
    26641 ccgagcgatg ttgataacta ccgtggcgaa cgagcaagaa aaggctcaca gctagtgaga
    26701 gggcttagta ccaattcaac ggtagagatt gttgagtaaa gagaagaagc ggttaggggg
    26761 taaatccaac aattttgccc caaataaccg cttgcaatgg agttattgct tattcaaata
    26821 agcctttgtg aagcgaacga accacatcgt ctgcttctgc atttttcact aaggtacaaa
    26881 cattattgct gcttgctcca tagctgatta ggcgaatgtt gtaattctca agagtgtgga
    26941 ataatttttt cgccacaccg gactgagcgt gtaagttgtt gccgataata gcgactaaag
    27001 cgaggtcgct ttctacttgc acattgcaat atgcgttaag ctcatcgagt aattccgctg
    27061 ataacatatc tgcacctgaa gaggctgagc ccgttttatc tagggtcaat gcaacgctga
    27121 cttcggaagt ggtaatcaca tcaaccgaaa ttttgtgttt cgctaaaatc gcaaatacat
    27181 tcgctaagaa accttgtgcg tgtagcattg aaaggctgga aagcgtgagt aaaatttggt
    27241 tgcggcgtaa tgcaatcgca cggaaagtcg ggcgtggttg tggatcacgg gttacccaag
    27301 tgccgccttg ttccggtgct ttgcttgagc caacatatac agggatattg ctacgcactg
    27361 ccggtaataa ggttgctggg tgtaatactt tcgctccaaa tgttgccatt tctgccgctt
    27421 cgttaaatgc cattgtgtca atgcgttgag cgttcggtac aacacgagga tcggtggtat
    27481 aaatccccgg tacgtctgtc caaatgagta catcgttagc atttaatact tctgctaaca
    27541 atgcagcaga gtagtctgaa ccgccacgtc ctaaagtagt ggttttgcct tcatcatcac
    27601 gaccaataaa gccctgtgta atcacaattt tgcctgatgc gattaatggc ttaatcacat
    27661 tgtctgattg ttgctgggtt ttctcgtcat ttggtgctgc ttttccataa tggctgtttg
    27721 ttgccacaac gtcacgcaca tctacccata ctgccgggtg tccacgttca attaataatt
    27781 gcgtgaagat tttggtggac atcatttccc catggctgat taactcatca cttaaggcag
    27841 ctgaagtagc aaggctggct gattctgcta atgtttcaat acgttctaat aattcgtcga
    27901 tttccgcttt aacggcatct tgatgttgta atttttcaat aatattgtat tgaatttggc
    27961 ggactgctgc gatgacttca tcacggtgtt ctttttcaca gccgttagca agttccacta
    28021 agtaattggt tacgccagca gaggcagaaa gtaccaccac acgagtattt gcatcagcaa
    28081 ttacaatatt ggcagaagag gtcattgcat caaaatttgc aacacttgta ccaccaaatt
    28141 tagccacaga aagatgagac ataataaaaa tttccttaaa acaaaaatag ataaatattg
    28201 tgttagggga tattcggaaa ggaaatttga gtagattata cggttttatt gttgaaccaa
    28261 ttaatcagag cactccattt aatatggtga aataacctat taaatgacag ctgataggat
    28321 tcaacctatt cagccgatgt taattccagt ctagtgaatt cacacctcgg caatcactcc
    28381 catcacttaa ttctcaaacg gccctagttg cctaaaatca agttgcctga gtgttgcacc
    28441 tctcccgtaa tggcaagccc attactcttt ttcaacagta ttgaaaagtg tgcatagaaa
    28501 taccgtttta ttaatgtttt gtcaatg
//
LOCUS       DANLXQ010000014        27400 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.014, whole genome shotgun sequence.
ACCESSION   DANLXQ010000014 DANLXQ010000000
VERSION     DANLXQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27400
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.014"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(266..448)
                     /locus_tag="PXD94_000473"
     CDS             complement(266..448)
                     /locus_tag="PXD94_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285368.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            598..1638
                     /gene="selD"
                     /locus_tag="PXD94_000474"
     CDS             598..1638
                     /gene="selD"
                     /locus_tag="PXD94_000474"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:643..645,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL6285369.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
     gene            1638..2111
                     /locus_tag="PXD94_000475"
     CDS             1638..2111
                     /locus_tag="PXD94_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL6285370.1"
                     /translation="MFKPNVTMACVVHCKGKFLFVEEIEYGKRTLNQPAGHLEKDETL
                     LEGASRELFEETGIRAEMANLVKIYQWQAPRSQTDFLRFLFSVELEEWLEPNPQDADI
                     TQALWLTLAEFEDYIQQQGQCARSPLVVQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            2101..3309
                     /gene="ubiH"
                     /locus_tag="PXD94_000476"
                     /gene_synonym="visB"
     CDS             2101..3309
                     /gene="ubiH"
                     /locus_tag="PXD94_000476"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL6285371.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            3572..4804
                     /locus_tag="PXD94_000477"
     CDS             3572..4804
                     /locus_tag="PXD94_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL6285372.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            4871..5857
                     /locus_tag="PXD94_000478"
     CDS             4871..5857
                     /locus_tag="PXD94_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL6285373.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKPFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNTLPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVKGIGVFKEILSE"
     gene            complement(5898..6308)
                     /locus_tag="PXD94_000479"
     CDS             complement(5898..6308)
                     /locus_tag="PXD94_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL6285374.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(6374..7684)
                     /gene="eno"
                     /locus_tag="PXD94_000480"
     CDS             complement(6374..7684)
                     /gene="eno"
                     /locus_tag="PXD94_000480"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL6285375.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVAKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(7875..9053)
                     /gene="rlmC"
                     /locus_tag="PXD94_000481"
     CDS             complement(7875..9053)
                     /gene="rlmC"
                     /locus_tag="PXD94_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDL6285376.1"
                     /translation="MSSLLHCSHFERGDCTSCQWIEKPYSTQLAAKEADLKRLILPFI
                     LQNKTEILPLVGSPISQFRNKAKMVVSGSVERPILGILKDQNDAQSGVDLTDCLLYPS
                     SFHRLFPILKDFIARAGLVPYNISKKKGELKYILITESQYNQSFMLRFVVRSEAKRAL
                     VERELPELLEKLPPNSVVSLNIQPQHAALLEGETEIFLTEQKVLEEHFNGIPLFIRPQ
                     GFFQTNPVVASQLYHTAQSWIEHLPIAKLWDLFCGVGGFGLHCANALQAKNSNVELTG
                     IEISASAIASATKSAEKLGLKQVKFASLDSAQFALNEAGSPPDLVIVNPPRRGIGKLL
                     AEFLNQLGSPYLIYSSCNAVSMANDFTFLTNYELHKVQLFDMFAHTAHYETLTFLVKK
                     "
     gene            complement(9046..10083)
                     /gene="nagZ"
                     /locus_tag="PXD94_000482"
     CDS             complement(9046..10083)
                     /gene="nagZ"
                     /locus_tag="PXD94_000482"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL6285377.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFNQLATTPEQAMQLAKETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGQEPAAILPVAEAFIDGMREIGMATT
                     GKHFPGHGHVLADSHLETPFDDRPKERIFNHDILPFKQLIAKGKLSAIMPAHVVYTQC
                     DSQPASGSSYWLKEVLRKQLNFNGVIFSDDLGMKGAGFMGNFVERSEKAIQAGCDLLL
                     LCNEPEGLVQVLDGLKYQPTQAQKERRLSLMKRKTVTWRELENSSRYQQTQAELTALQ
                     NNWLEWKEANV"
     gene            complement(10209..10940)
                     /locus_tag="PXD94_000483"
     CDS             complement(10209..10940)
                     /locus_tag="PXD94_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL6285378.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAAVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            complement(11029..11709)
                     /gene="mutH"
                     /locus_tag="PXD94_000484"
     CDS             complement(11029..11709)
                     /gene="mutH"
                     /locus_tag="PXD94_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL6285379.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     IWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            complement(11801..12436)
                     /gene="lexA"
                     /locus_tag="PXD94_000485"
     CDS             complement(11801..12436)
                     /gene="lexA"
                     /locus_tag="PXD94_000485"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL6285380.1"
                     /translation="MRKHLTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAA
                     EEHLKALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEH
                     VENHYPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVND
                     EVTVKRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
     gene            12603..15038
                     /gene="plsB"
                     /locus_tag="PXD94_000486"
     CDS             12603..15038
                     /gene="plsB"
                     /locus_tag="PXD94_000486"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL6285381.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRSLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLGSYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGYLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            complement(15143..15706)
                     /locus_tag="PXD94_000487"
     CDS             complement(15143..15706)
                     /locus_tag="PXD94_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL6285382.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTSQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(15904..16800)
                     /locus_tag="PXD94_000488"
     CDS             complement(15904..16800)
                     /locus_tag="PXD94_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6285383.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDWQLENPHQTGQAIAHIIS
                     YGQAYLQHNGKTHHLKQGDIVFFPRFADHTLRSQIQPLTSKNAVNPEQLGAFTLTQAG
                     LSQSECDMFCLHFYYDHQAELMESLPEMIVFQVAETPLKAMLDILKQEAETPNLAVKS
                     VVNSLAVVILTMILREHLVNHNNQLFGILKGWQDLHLRPLIKAILLNPEHEWQVDDMA
                     AFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAMLLKSQAGSVLSIALSCGFQSES
                     HFITAFKKQYCVTPSQYRQLNP"
     gene            16936..17277
                     /locus_tag="PXD94_000489"
     CDS             16936..17277
                     /locus_tag="PXD94_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL6285384.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            17288..17641
                     /locus_tag="PXD94_000490"
     CDS             17288..17641
                     /locus_tag="PXD94_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL6285385.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            17701..18339
                     /locus_tag="PXD94_000491"
     CDS             17701..18339
                     /locus_tag="PXD94_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL6285386.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            18474..19655
                     /gene="purT"
                     /locus_tag="PXD94_000492"
     CDS             18474..19655
                     /gene="purT"
                     /locus_tag="PXD94_000492"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL6285387.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSDIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(19747..21240)
                     /locus_tag="PXD94_000493"
     CDS             complement(19747..21240)
                     /locus_tag="PXD94_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL6285388.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFPPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVFFLIWPAFQQWLVSAGQGIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPL
                     FWYTELGGTEVVNGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAA
                     CLAMYHAVPKARRGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVS
                     FYIADLLNISIGNTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLI
                     KKFNVMTPGRAEESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKE
                     VKAVDKATLKKLGAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
     gene            21441..23288
                     /gene="ilvD"
                     /locus_tag="PXD94_000494"
     CDS             21441..23288
                     /gene="ilvD"
                     /locus_tag="PXD94_000494"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL6285389.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(23422..23772)
                     /locus_tag="PXD94_000495"
     CDS             complement(23422..23772)
                     /locus_tag="PXD94_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL6285390.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(23801..24466)
                     /locus_tag="PXD94_000496"
     CDS             complement(23801..24466)
                     /locus_tag="PXD94_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL6285391.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            24672..25220
                     /gene="pgsA"
                     /locus_tag="PXD94_000497"
     CDS             24672..25220
                     /gene="pgsA"
                     /locus_tag="PXD94_000497"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL6285392.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPTKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            25298..27136
                     /gene="uvrC"
                     /locus_tag="PXD94_000498"
     CDS             25298..27136
                     /gene="uvrC"
                     /locus_tag="PXD94_000498"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL6285393.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALVLQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(27141..27338)
                     /locus_tag="PXD94_000499"
     CDS             complement(27141..27338)
                     /locus_tag="PXD94_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285394.1"
                     /translation="MDMQAVQDYHQRVINKQTVDKNTKQHDEPLNQSDKRSESINLPI
                     HPIIYPSIGVGYHYGWKRYRY"
BASE COUNT         7896 a         5338 c         6146 g         8020 t
ORIGIN      
        1 gaaaacggat ttgggtggaa aagaatttaa gctcaaatta atctgacaga cacggtaatt
       61 taaaacggga gactgtcaga tcaagtctga tcttctacaa ctaagccttc tcagaataaa
      121 taaattttca caagcggtgc aactttgcaa aaacttcggt attatgtagc agttgcacaa
      181 aaccccaaat tcattaaaac cgcttaaaaa tgaaatgccc taaactgatt tttcagtcta
      241 gggcatttat tatcacatat tatggttaat agggaaaact cacatcattg ttatgctggt
      301 gatatttttg ccaaaatgcg tgatattgtt ttttcaccga tgtgtttaat aaaaacggga
      361 ttatcaagac ataggtcggg caattccata gcattgcacg ttgtgtatcc gtgccgattt
      421 tggtgcgtaa gtaaccaaac tgatacatta gacccaatcc aaccgtcacc tattctaata
      481 gcataataac taaggtcgca tcattaaatt aaaagctcat tttttgcaaa aaatttccca
      541 aattccaccg cttgttggcg tacaataggc ataattttga tttacgatgg aggcagaatg
      601 tctgaaccga taaaacttac gcagttcagc cacggggcgg gttgaggctg taaaatttcg
      661 cctaaggtgt tagggacaat tttacaaact caattagaac aatttgtcga tccgaattta
      721 ttggtaggca atgaaacggc ggacgatgct gccgtctatg atttagggaa cgggcaggcc
      781 attatcagca ccaccgattt ctttatgccg attgtggacg ccccgtttga tttcgggcga
      841 attgctgctg ccaatgcgat tagcgatatt tatgcgatgg gtggcaagcc gattatggcg
      901 attgcgattc tgggctttcc ggtgaataaa ttgccggctg aagtggctca acaaattgtc
      961 gaaggtgggc gttttgcgtg ccagcaagcc ggtattgcct tggcgggcgg gcattcgatt
     1021 gattcgcccg atccgatttt cggcttggct gttaccggca ttgtgccgac cgaacgggtg
     1081 aaacgcaatg cctctgcggt ggctgggtgt gagctgtttt taaccaagcc tttggggatt
     1141 ggcattctaa ccacggcaga gaaaaaaggt ttgttgaagc ctgaacatca acatctggca
     1201 agcgaggtaa tgtgccaaat caacttgatt ggttcagcgt tttccgaact gcctgaagtc
     1261 acggcaatga ctgatgtaac cggttttggc ttactcgggc atttaagcga ggtgtgtcaa
     1321 ggctcgggct tacgggcgga ggttgatttt gaggatattc aagtccttga tggcactaag
     1381 gagtatattg cattgggagc agttccgggt gggacaaatc gcaatttcga cagctatggg
     1441 catttgattt cggcaatgac ggacgagcaa aaagcgattt tgtgcgatcc gcaaacctct
     1501 ggcggtttat tagtggcggt gctgccacaa gcggtcgaaa aagtgcaaca aattgcaaat
     1561 cgggcaaatg tgccgttatt ccacgttggc agattgctgg aaatggaaca gggcaaacca
     1621 cggattgagg taatttgatg tttaaaccaa atgtaacaat ggcttgcgtg gtgcattgta
     1681 agggaaagtt tctgtttgtg gaagaaattg aatatggcaa acgtaccctt aaccagccgg
     1741 cagggcattt agaaaaagat gaaaccttgc tggagggggc aagccgtgag ctgtttgagg
     1801 aaaccggtat tcgggcagaa atggcaaatt tggtgaaaat ttatcaatgg caagcccctc
     1861 gttcacaaac cgatttcttg cgttttctgt tttcggtgga actggaagag tggctggagc
     1921 cgaatccgca agatgcggat attacccaag ccctttggct tactttagca gaatttgaag
     1981 actacattca gcaacaaggg caatgtgcca gaagcccgtt ggtggttcaa tcggtcaaag
     2041 attatttaag cggtgagcgt tatccattaa gtttgttgaa tgcgtttgaa tgggcgagaa
     2101 atggaaaata aaacaacaac ccaatttgat gtggtgattg tgggcggggc agtgaccggc
     2161 tcggtgctgg cattggcgtt aagtagtttt actcatcaca aaatctcgat tgcgatagtg
     2221 gaaaaatcgc tgccggacta tgaacagcaa ggcggttttg atgctcgcag tattgcctta
     2281 gccgaaggca gtttgcaaaa aatggggcaa atcagaccgc ttgcaggctc aagtttaggc
     2341 gagatgatta gcgaaatcgg cacgccgatt cagcaaattc aagtagcgga taaagggcat
     2401 tttggtaaaa cgaccttgaa agcaagcgag cagaatgtgg ctcaactggg tatggtggtg
     2461 gaacttgcaa aattcggcag aaaattgacc gcttgtcttg cacagcaagc taatattacg
     2521 cttttttgcc cgaatcaagt ggttggcatt gagcgttccc aacaacattg tggattgagc
     2581 ttgaaaaatg gtgagaaact aagctgttcc ttgctggtgg cggcagatgg tattcaatct
     2641 cagattgcaa aacagtgcgg tgtggaaacc gtaaaagtaa gggattatgc acaatcggca
     2701 attatcgcca atgtggaact gagtgaatct caccaaaatc aagcctttga atattttacg
     2761 cctcaaggcc cttttgcttt attgcctttg agcggaaata tgatgtcgtt ggtgtggtgt
     2821 gtgaaagagc cgaacgagtt aatccaactt tcagacagtg aatttttagc ccgtttgcaa
     2881 caacaatttg gttggaagtt agggcaattt gttcgagtga gcaagcggtt tgtttatccg
     2941 ctcattttgc aaaaagccca gtcgcatatt caccaccgct tagcagttgt cggcaatgcc
     3001 gcacaaatgc ttcacccggt tgctgggcaa ggtttcaact taggcttgcg tgatttgact
     3061 accttagcgg aattagtggc tttggcgttt aatcaaggga aagatatagg cgaattttcg
     3121 ctcttatcaa cttttgagaa tgagagaacg caagatcaag ccagaatgat aaatgctaca
     3181 agcggtctga tttcgctttt ttgttgcgaa atgttaccag ttcaggttgt gaggaattta
     3241 gggttattaa cggtttccca ttcaaaaata gccagagatt ttgttgctaa tcaggcatta
     3301 ggttggtaaa aaacacaaaa gcagcaattt tctatttgac actgttttta aaaaggcgta
     3361 ttatccaacc cgtttaaaaa tctcaccaaa gccttgaatg caaattcaag gctttttcct
     3421 ttttagtgaa agcctatgaa ttttgtttgc cgtaaaatcg gcaaaaaaat tttgcctaaa
     3481 attcgcaatc gtttgcgaaa taggtttata gcgttgtttt catctgggta actgcaatgg
     3541 ttacatcgca atacatcaaa gagagtaaaa aatgaccaca gaaaatatga gtacgccaaa
     3601 gcaggcgttt atcgggctgc aaatgctgtt tgttgcattt ggggcgttag tattagttcc
     3661 gctgattacc ggtttagatc ctaatgttgc ccttcttacc gccggtgtcg gtacgttaat
     3721 cttccaaatt tgtactaaac gtcaagttcc tgttttcctc gcatcgagtt tcgcttttat
     3781 cgctccaatc caattcggtg ttcaacaatg gggaattccc gtcacaatgg gagcattgct
     3841 ctgttccggt tttatttata tggcattatc tgctttagtg aaagcgaaag gggtgaatat
     3901 tctgcataaa tacttccctc caatcgtgat tggaccggta attatggtga ttggtttagg
     3961 tcttgctcct gttgcggtgg atatggcaac gggtaaatcg gcgaatattg cctataacac
     4021 cgctattatt gtttcaatga taacgctggt aactacgctg attgtggcgg tgtttgcgaa
     4081 agggctatta aagctcgtgc caatattagc aggtttaacg gtgggatata tcttgtctgt
     4141 ttttatggga ttggtggatt ttactaaagt ggttgaagct ccttggttta gtctaccgaa
     4201 agtttccgca ccggaattca aattagatgc aatcttgtat ttattaccta tcgcacttgc
     4261 tccggcagtc gaacacttag gcgatgtaat ggcaatcagc caagttgccg gtaaagattt
     4321 tatgaaaaaa ccggggttac accgcacgct tttaggtgat ggtttagcaa cttcagcagc
     4381 tgcggcatta ggtggcccac caaatacgac ttactctgaa gtaaccggtg cggtaatgct
     4441 aaccaaaaac ttcaatccga aaattatgac ttgggctgcg tgctgggcta tcgctttctc
     4501 attctgcgga aaaatcgggg cattccttgc cactatccct acttttgtga tgggcggtat
     4561 tatgatgttg ctgttcggtg cagttgcggt tgtggggatc aatactctaa ttaaagctaa
     4621 agttgattta agcattccac gtaatctttg tattgcttct gtagtaatga cattcggtat
     4681 tggcggtatg ttaattaaca tcggcgaatt ctcattaaaa gggatcagct tgtgtgcatt
     4741 agtcgctatt gtacttaaca ttgtattacc aaaagaaaaa gtagaagatt ctgctgctca
     4801 ttaatcaaag gacgctgtgg cgtccttttt gctaataacc ctctatttgt aaataaaaag
     4861 gaataacact atgcaaaaac tcacattttc accaccactt ttaaatacgc cttgcccttg
     4921 gtgttcggag ttagagagct tgcgtgaact ttatgcgtgt gaatataccg gtgcggtaac
     4981 aacccgtact tcaatgcctc aaccatatcc acacgattgg gcgaagaacc aatatgtact
     5041 ctttgattcc aacgcacaga aaacagcaag tgtgaatact caagatgcaa ctagcttaca
     5101 aacagcaagc cttaacacca tcggtttaag cccaaataat ctggatacca ccatcagctt
     5161 tgtacgcacc atttcaaatg agctgacagc gccatcaagc aaaccattta ttatttcggt
     5221 gtttggttca cccgaagagg tgggggaatg ttatgaaaaa attgttgctt tccaaactga
     5281 agtgaaaatg cctttggcaa tggagattaa tatctcttgc ccgaatattc cgggtgaaat
     5341 ttcgcctgct tattccgcag aagaattatc acattacttg cacgcactac aaacttcgct
     5401 aaaaaaactg gctcaaggtg ataatacttt accaatcggg attaaaattc caccttttac
     5461 ttaccaaaat caatatgatg aattaatcaa aggcttatta caagcggtcg aaaatggcga
     5521 aaatttacca atttccttta ttacttcaac caatacgctt ggctcatcgc ttgtcttaaa
     5581 tgctggcaaa gcggtattaa attcagaagt gggaacgggc gtaggcggaa tggcaggaac
     5641 ggcaatccat gcactttctt tggggaatgt ttacacgatt cgccaaatgt tagatcaaga
     5701 gcctaagctc aaacatcttc aaattattgg tgtgggcggc gtgaatgata aagccggctt
     5761 tgagcgaatg aaatcggttg gggcagactt tgtggggtta gctaccgcat taggggtgaa
     5821 aggaatcggc gtttttaaag agattctgtc tgaataatga agaggagggc gtgcattgca
     5881 cgcccgaata aatgaaatta gcctaaccat ttctccacta attcattagt ggtttcttta
     5941 tgtacccaca tactgccaag ttccggcatt gggaatttgg tgtggttgta gccgacaaat
     6001 tggcggaaat caaacacggc gtgcgggtag ccgttgatca gtaaaaaggc ttgatagccg
     6061 tcttcactcc agcaaatttc taaacggcga ggttctttta atgaattttg ctcaatatcg
     6121 ctgacccgaa acacaaataa actatccact accggctgtt cggtttggtg taagtccatt
     6181 gcataaaagt agccgctttc ttcatcaatt tcaaacatca ctactaaatg ttcgtgctgg
     6241 gttgaatgag cgccattacg gtaaggttcg ttcagtagaa tatgagcagt taaaacagag
     6301 tagagcatta aattatcctt ataagtaaaa tgccaccgat ttctcagtgg catttaaata
     6361 tttaatcagg caattacgct tgacctttaa tctctttacg gccgttgaac ggagccggtg
     6421 taccagcacg ctctaacgct tcttcgatac ggattaactg gttgtatttc gctacacggt
     6481 ctgaacggct cattgaaccg gttttgattt gacctgctgc tgtacctact gctaaatctg
     6541 caatggttgc atcttcagtt tcgcctgaac ggtgagagat aactgcggtg taacctgcat
     6601 ctttcgccat tttgattgca gctaaagttt cagttaaaga accgatttgg ttgaatttga
     6661 ttaagattga gtttgcgata cctttctcaa taccgttttt caagattttg gtattggtta
     6721 cgaataagtc atcaccaact aattgtactt tgtcgcctaa tactttagtt tggtatgcga
     6781 aaccttccca gtcagattcg tcttgaccat cttcgataga cacgattggg tactctttgc
     6841 ataagccttc taagtagtga gtgaactctt gtgaagtaaa ggttttgcct tcgcctttca
     6901 tatcgtacac attgcgttct ttgtcgtaga attcagatga tgcacagtcc atcgctaaag
     6961 ttacgtcttt acctaacaca tagcctgctt tttctaccgc ttctttaatg cacgctaatg
     7021 cgtctgcgtt tgaagccaag tttggtgcga aaccaccttc gtcacctaca gcagtattta
     7081 agcctttcgc ttttaatact tttgcaagat tgtggaatac ttcagcaccg atacgaagag
     7141 cttcttttaa tgtagaagca cctaccggtt gaatcataaa ttcttgaatg tccacgttgt
     7201 tgtcagcgtg ttcaccacca ttgatgatgt tcatcattgg taatggcatt gagtaaacac
     7261 ccggtgtgcc gtttaattca gcaatgtatg cgtaaagcgg taagcctttt gaagcggctg
     7321 ctgcttttgc tgttgctaaa gaaaccgcta agatagcgtt tgcaccgaat tttgatttgt
     7381 tttctgtgcc atctaaatcg atcataattt ggtcgatttc agcttgtgca gaaccgtctt
     7441 tgcctactaa tgcgtttgca atttcgttat ttacggcaga aaccgctttt aatacacctt
     7501 tacctaagaa acgacctttg tcgccatcac gtaattctaa tgcttcacgt gaaccggtag
     7561 aagcacctga tggagcggct gctaagccta caaaaccacc ttctaagtga acttctgctt
     7621 ctacagttgg gttaccacga gagtcgatga tttcacgacc gattacttta acaattttag
     7681 ccattgttga tgtcctctaa tatacgagtt aatagaaaaa agaataggta tatagtaaag
     7741 cgattttctg cttttgtctt ggaaaaactg attgagtggc agaaaatttg ccactaattt
     7801 gatctaagac tatattcgcc tgagattata gcaacctttg caaaaaacgg caaaaatttg
     7861 cccgcttgta atgcttattt tttgactaaa aaagtcaagg tttcataatg tgctgtgtga
     7921 gcaaacatat caaacagctg cactttgtgt agttcgtaat ttgtcaaaaa tgtaaaatcg
     7981 ttcgccattg agacggcatt acaactggag taaatcaaat aaggtgagcc taattgattt
     8041 aaaaattctg ctaatagttt gccgatacca cgtcgaggtg ggttcacaat cactaaatcg
     8101 ggtggagaac cagcttcatt taaagcaaat tgtgcagaat ctaatgaggc aaattttacc
     8161 tgctttaggc cgagtttttc agccgatttg gtcgcacttg caatagctga ggctgaaatc
     8221 tcaatgcctg tcagctccac gttcgagttc ttcgcttgca aggcgttggc acaatgtaag
     8281 ccgaaaccac cgacaccgca gaacaagtcc cacaatttag caataggtaa atgctcaatc
     8341 cagctttgtg ctgtgtggta aagctggctt gctaccacag ggttagtttg gaaaaagccc
     8401 tgagggcgaa taaatagcgg tatgccgtta aaatgttctt ccagcacttt ttgttcggtg
     8461 aggaaaatct cagtttcgcc ttctaaaaga gcagcgtgtt ggggctgaat attcagacta
     8521 actactgagt tgggtggcag tttttccaac agttcgggca attcccgttc aaccaatgca
     8581 cgctttgctt cactacgaac cacaaaacgg agcataaatg attgattata ttggctttcg
     8641 gtaatcagaa tatattttag ctcacctttt ttcttactga tgttatacgg cactaaacca
     8701 gctcgagcga taaaatcttt caaaatcgga aaaagtctat gaaatgagga cgggtagagc
     8761 agacagtcgg ttaaatccac cccgctttga gcatcatttt gatcttttaa aatgcctaaa
     8821 atcgggcgtt ctacactgcc ggagaccacc attttcgctt tgttacgaaa ttgcgaaatt
     8881 ggcgagccga caagcggtaa gatttccgtt ttattttgca aaataaacgg caaaatcaac
     8941 cgcttgagat cggcctcttt ggcggctaat tgcgtgctgt atggcttttc aatccactgg
     9001 cacgaagtac agtcgccacg ctcaaaatgc gaacaatgca acaagctaga cattcgcttc
     9061 cttccattcc agccagttgt tttgtaaggc cgttaattca gcctgagttt gttggtagcg
     9121 agagctgttt tccagctctc gccaagtcac ggttttgcgt ttcattaaag aaaggcgacg
     9181 ttctttttgt gcttgagtcg gctgatattt cagcccgtct aacacttgca ccaggccttc
     9241 aggctcgtta caaaggagca ataaatcaca gccggcttgg attgcttttt cagaacgctc
     9301 gacaaaattg cccataaagc ccgcaccttt cattcccaaa tcgtcagaga atatcacacc
     9361 gttaaagttc agctgtttac gcaatacctc tttaagccaa tagcttgaac cgcttgccgg
     9421 ctggctatca cattgggtgt aaacgacgtg ggcaggcata atggcagaga gttttccttt
     9481 ggcaataagc tgtttgaacg gcagaatatc gtgattaaaa atccgctctt tcgggcgatc
     9541 atcaaacggg gtttctaaat gcgaatcggc taaaacgtgt ccatgaccgg ggaagtgttt
     9601 gccggtggtc gccataccga tttcacgcat tccgtcaata aaggcttcgg caacaggcaa
     9661 aatcgctgct ggctcttgcc cgaatgagcg atcgccaatc gctttgcatt cgtgtcctaa
     9721 atccagtacc ggagcgaaac tgaggtctat atcgagggca aacatttctg ctgccatcaa
     9781 ccagccggtt tctttcgcta attgcattgc ttgctctggc gttgtggcaa gttggttaaa
     9841 agattgcatt gccggcagtt tagtaaagcc ttcacgaaag cgttgcaccc gaccgccttc
     9901 ttggtcgaca gtaataagca acggtttttt cacccgctgg cgaatggatt tcactaatgc
     9961 ctgaatttgt gctttgtcgt agaaattacg ggtgaataaa attagcccgg aaactaaagg
    10021 gtgttccagc atttcgactt cttcttgagt cagttcttta tctttaatat caatcagtaa
    10081 cataattttc gttgagctat ttgcaaaaaa atgagcagat atgaccgctt gttgtgccta
    10141 ttctacatta aaaagccctt gatgttgatc aagggcgaat gattaagggc ataaatgaga
    10201 cagagcaatt atgcttgttc taatttagct tggttacgac gcatttttat gttgagcatt
    10261 tcaaccacaa ccgagaagcc cattgccgta taaaccgctg cttttggaat atgaatatca
    10321 aaaccttcgc cgactagtac aataccgatt aaaatcaaga acgctaaagc gagattttta
    10381 atggttgggt tgttatctac aaagtcccca atggttttcg ctgctaacat cattaccgca
    10441 actgcaatga taatcgcaat aaccattacc ggaatatcat ctgccattgc cacagcagta
    10501 atgactgaat caagggagaa aataatatca aaaattgcaa tttgaattaa caccataaaa
    10561 aagctggctt ttttaatgga ttcgcttttt tcttcgtgag attcggcaga aatggattct
    10621 ttcagctcca ttgcactttt aacaatcagg aataatccac cgatgagtag aatgagatct
    10681 ctacctgaaa ttggattgtt gccaatagtg aaaagaggct cgaccaaacg catcatccaa
    10741 gcaagcgaga gaagcaataa aatacgggtt cccattgcca gccccaagcc gataattcgg
    10801 gcagattggc gttgttcaat aggtaaacgg gaaacgagaa tactgatgac gatgatgtta
    10861 tcaatgccta atacgatttc aagcccggta agtgtaaaga gcgcaaccca agcttcagga
    10921 tttgtgatcc agtcaaacat aaaaatgtcc ttttaaaaat aagggattaa gtgatatttt
    10981 ctatcaaata gaattaaagc cgacatctta tagagtgtga ggacaaattt aaagggggca
    11041 atgcaaaaag ttttgtaaaa tttcggcggt aaaatgttta cgcaaataga aaccaagagg
    11101 gagagattga acaatctccc ctttagcgtt gatagcagag gtgatagagc ggttatttct
    11161 acctttgggt ctgagttgta acacttcgcc taaggttgca ttaattttat gcagttcacc
    11221 taacacaatc aactccatta attcctccca atcttgctgt aggcgggatt cttgtgtgat
    11281 agtcggagac cataaaatcg gctgaccgat atggcgttcg gcaaggggta tctttcgttc
    11341 cccttcaacc ggaatccaaa gcacacggga aagcttatgt ttaacgtggg aggtttccca
    11401 aataataccg ttgttttggg ttaaaggagc aaggctgacg aatgtggttt ccagcggtaa
    11461 tcctttctga ttgaccggaa tggttttcag ctcaatgcct aaatgtgcaa aatcctgttc
    11521 ggctttactg ccggctttcg cacctaaagc ggtttcaatc agagtaccga cccagccttt
    11581 atctcgtttc agatctgctg gtatcggaat atgaagcatc tcggcaattt cacctaaagt
    11641 gaaccctgcc agccattccg ctttgcttaa taatgcctgc tcggttggag agaatgtaga
    11701 taattgcatt gcacttagtt aattagacta aactggcaag cggtcaaatt taggcagaaa
    11761 tttgcaaaaa attactaaaa aataaccgct tgtgaggaaa ttacatccaa gcattactgc
    11821 gaataacgcc tactgcaatg ccttcaattt caatatattc ttgagcaggg tttactacaa
    11881 tcggttttaa ttcatcgttt tcagggtgta agtaaattaa ttcacctttc ttctctaagc
    11941 gtttaacagt cacttcatca tttacccgag caaccaccac ttgcccgtta cgagcggtat
    12001 tggttttatg aacggctaat aggtcaccgt ctaaaatacc gattttttcc attgaattgc
    12061 catttacttt gagtaaataa tcagcacttg gattaaacat cgcaccattc accgggtaat
    12121 ggttttcaac gtgttcaacc gctaaaatag gtgtgccagc agctactttg ccaatcaacg
    12181 gtaagccttc atcttcatcg ttggcagctt ggttttcgtc ttcgactaaa atgcgaattc
    12241 cacgagatgt acctgataac atttcaatat agcctttacg agcaagggct tttaagtgtt
    12301 cttctgccgc attaggtgat ttaaagccga tttcacgggc tatttctact cgggttggtg
    12361 gcataccggt ggtttcaata tggtttttga cgaaatcaaa aatttcttgc tgacgggcag
    12421 ttagatgttt acgcatttta agttaatcct cactgtcttt atatacagaa aatattgata
    12481 gtatatacag tatttttgat tttgtaacta ataatttcac gaaattccat aaaaatttat
    12541 ggtagaatgg aagaaattag aggttagacc tccaacaata acgattctaa aaggatattt
    12601 atatgtctgg cttattaaat ttttatcgag ctttcctaaa cttaccactt tcacttttag
    12661 taaaatctcg ctctattccg actcatccgg tggctgaact ggaactaaat cttgaacagc
    12721 cgattgtcta tgttctacct tatacctcgc aaaccgattt attaatttta caaaaaaact
    12781 gcttggcttt gaatttgccc gatccgttgc aagaaaatgt gattgaaggg caaacattgc
    12841 ttcgttttgt atttttagat gaaggtcgcc gtttttttaa atctaaaggg gcgaaaagtg
    12901 agacagagtc gattttttat cgttatttag atttacatcg tgctaatgct gaattagacg
    12961 tccaattagt gccggtttcg gtattatggg ggcgggctcc gggcaaagaa gatgcccgcc
    13021 atttacaggt gcttacctca ttccaacgtt ttttatccat ggtttggttt gggcgtgata
    13081 attttgtacg tttctcccct gcagtatcgt tacgctatat ggtaactgaa tatggtgcag
    13141 atgaaaaaat cgctcaaaaa atcgcgcgtg tggcgaaaat gcactttgca aaattgcgtt
    13201 attcagcaat ggggcctcgt ttaccaaatc gtgatgcgat gtttaataaa atcctgaatt
    13261 cagaggtgat tcaagctgcg attgcggaag aagcgaagaa atcatcgcct gaaaaggctc
    13321 gcaaagaagc cgagaaaatc attaatgaaa ttgccgctga tgtaaaacac gagagtttgc
    13381 gtgtagcaga tcgagttctg agctggcttt ggaataagct ttatcaaggg atcaacgtgc
    13441 aaaatgcaga ccgtgtgcgt aaattggcat tagaaggtca cgagatcgtt tatgtgcctt
    13501 gccaccgtag ccatatggac tatttgctcc tatcttatct actttaccat caaggattag
    13561 taccgccaca tattgccgct gggatcaatt taaatttctg gccggcaggc ccgattttcc
    13621 gtagttgggg agcatttttc attcgccgta cctttaaagg aaatcgcctc tattccacca
    13681 ttttccgtga atatttagcg gaattattct atcgtggtta ttcggtcgaa tattttattg
    13741 aaggcgggcg ttcacgcaca ggtcgtttat tagagcctaa aaccggtatg atgtcgatga
    13801 gcctacaagc cttacagcga gggctgaata gatctttaag tattgtgcct gtttatatcg
    13861 gttatgaaca cgtcttagaa gtcgatactt acgctaaaga gttgagaggt gcggcaaaag
    13921 agaaagaaaa tgcgggcttg gtgctacgag tgctgaaaaa actgaaaaat ttaggtcaag
    13981 gatatgtgaa tttcggtgag ccaattttgg tgaataatta cttaaatcaa tatttccctg
    14041 agtggaaaga gccttccgaa gatggacgac ctaagtggtt aaatgaagcc gtggataata
    14101 tttcacatca agtaatggtg aatatcaata aagcggcagc agttaatgcg aaaaacttag
    14161 tggggtcagc attattggca tctcgccagc gtgcattaac ccgtgaacaa ctcattgaac
    14221 aacttggaag ttatattcag ttatttcgta atgtacctta ttctaaagat atgacattac
    14281 caactgaaag tgcagaagtg atgttgaatc atgttattca tttacctcgt tcgggcgttt
    14341 taattgaaaa agataatttt ggtgaactgg ttcgtttaga acgagaatct gctgtgttaa
    14401 tgacatatta cagaaataat gtgcagcatt tatttgtgtt gccttcactt gttgcaagta
    14461 tggtacttca tcatgaggct gtttcgaaag atgttgtgat taagtcggtt aatcgtattt
    14521 atccattctt gcaagcagag ttattcttac actttaagca agaagaatta aaagcacata
    14581 ttgaagcaat tattgcggaa ctaagtcggc agggggtgat taaaaacgaa agtgatatgt
    14641 taatgatcaa tcgctcacat attcgtacct tgcaattaca ttcagctggt gtgcgtgaaa
    14701 tccttcaacg ttattatatt agcctaagtg tcttgttgga gcagccaaat attagtcgtg
    14761 gctatttaga aaaagagagt agttcaattg ctcagcgttt atcagtcttg catggtatta
    14821 atgcgccgga gttttttgat aaggcgattt tctctacctt tacatcaagt ctaaaagcac
    14881 aagggtattt tgatggtgat gataatttag cgattgcaaa agttaaagaa acggaatcgt
    14941 tgttacgtag tttgatttcg attgaagttc aacagaccat tcaaggtgcg atggctaaag
    15001 tcgcagaatc tgtagagaat cctaatatcg caggataata aatcaaagaa gggcgctaac
    15061 aagcgctcgt ttcttgcaat tttgttgcca aaaataaaag ctgaatgcgc gaccacattc
    15121 agctttttta cttcctatat aattattttg gttcaccgat tggtaaaaca gtcttaccaa
    15181 aactttcatt aataatatcc gcagcggcta agtagaacgc taagaatgcg gttaataaac
    15241 cgaagttacc accaatgtta gttagggtgg tgttatgggt aaaatcacct gcggctaaaa
    15301 ggtagaaagt aatggttaaa cttaagaaaa tagcttgtaa tgcacgggct tttgctagtg
    15361 tgccgatgaa catcataagt gtgaatgttc cccaagctac taagtaccaa ccaacgaatt
    15421 ctgccgatac attcgcagca aagaacactt taaataatgc aaaagaccac cagaatgcac
    15481 catagctggt aaacgcagta aagccgaacg tgttaccttt agcaaattca aacatacctg
    15541 caatcatttg tgatgtgcca ccgaaagcaa atcccattgc taaaactaaa cctacgtttt
    15601 caccgctgaa tgtaccgtta ttgattaggc ttagtaaaaa tgtagtaagt gcaaaaccgc
    15661 ataaaccgag cggagccggg ttagttcctg aatttggagc tgacattgaa aacctctgtt
    15721 atattgttac accataagtg tggtaattgt ttatttacgc cgataaatca gacgttatgt
    15781 gcctaaaatt tagggcgata aagtatagcg attttgcagt aaattgacta gaaatttccg
    15841 agacaagcgg tgagattttt gcaaaatttt gccaaaaaat gaccgcttgt tgttgtcatt
    15901 tagctatgga tttaactgcc gatattggct gggtgttacg caatattgct ttttaaaggc
    15961 ggtaataaaa tgcgactcgg attggaagcc gcaagataag gcgatactta ataccgaccc
    16021 agcttggctt ttgagcagca ttgctgcttt ttgtagccta attttgtgta caaaagcatg
    16081 tgggctaata cataaatgtt tgttaaataa gcggactaac tgggcacgag aaaggtggat
    16141 aaatgccgcc atatcatcga cttgccattc gtgttcggga ttaagcaaaa tagctttgat
    16201 caaaggtctt aagtgtaagt cttgccaacc ttttaaaata ccgaataact gattgttatg
    16261 gttaactaga tgttctcgta agatcatagt taaaataaca acagcaagcg agttaaccac
    16321 agattttacg gctaaattag gtgtttctgc ttcttgtttt agaatatcta acattgcttt
    16381 taatggtgtc tcggcaactt gaaatacgat catttcaggt aagctttcca ttaattcggc
    16441 ttgatggtca taataaaaat ggaggcagaa catatcgcat tctgattgag aaagtcctgc
    16501 ttgggtgagt gtaaatgcac ccaattgttc agggtttacc gcatttttgc tggtcaaagg
    16561 ctgtatttga ctacgtaatg tatgatcggc aaatctgggg aaaaatacaa tatctccctg
    16621 tttaaggtga tgggttttcc cattatgttg tagataagct tgcccatagc tgatgatatg
    16681 agcgattgct tgtccggttt gatgcggatt ctcaagttgc caatctccct gtaactgaca
    16741 tttcacactg attccggctg agatttgagc gaaagctaga agcctatcta atatatccat
    16801 tgttcattcc ttatatttcc accttaaatt gcgattattt cattataata tgagctgatt
    16861 tgataataaa gtttatcaga taaacgcaat aataaatgcg ttttttcata ttattcactt
    16921 taggaggaaa tcaatatgtc ttcattccaa aattggacag aacacacagc tcacgttaag
    16981 caatcttttg gtaaattagg gcaaaaacac cctaaaatgc ttgaagctta taaagcatta
    17041 ggtacagcgg ccgcagcgga agcattagat cacaaaactc gtgaattaat tgcattagca
    17101 gtagcgatta caacacgttg tgaaagttgc atcagtgtac acgcagaaga agcggcaaaa
    17161 gcaggtgcaa ccgaaagcga aattgccggt gcattagcga ctgcgattgc attaaatgcc
    17221 ggtgcggcat atacctatgc tttacgtgca ttagaagcgg tggaagagca aaaataagga
    17281 gcaataaatg aagaaattgg cttggatttt aggctttgca gctatgatgc caatgatgtc
    17341 tagtgctaaa caagcatcag ttcagcaagt gcaggctgaa aaagcagcgg gcatttggat
    17401 tgatgtacgt tctgctgaag aatttcagca agggcattta aatggtgcaa ttaatgtaac
    17461 gcatactgaa attgcacaac gtatcgcaga agttgctcca gataaaaatc agccgattca
    17521 tctttattgc cgctcgggtc atcgagctga agcggcgctt aatgagctaa aaaaattagg
    17581 ttacagcaat gtaactaatc atggtggtta tgacgatctg attaaagccg ggttgaaata
    17641 actgttttga gtgccaagca tttgcttggc acttttcgtt tatatctgag gttaaaaatt
    17701 atgagtgctt ttaacaattt tgttaatttt gttgcaaata ttgttgctcc aatgcaacgc
    17761 caattcatta actttgtacg tattgccatt tgtatcgtga tggtttggat tggtggttta
    17821 aaagtctgcc aatatgaagc agacggtatt gcccattttg tgtctaatag cccgttcttt
    17881 agttatatgt acaaaaaagg cccgaattta gttgatgacg gcacaggtaa aatggtgatg
    17941 gaatataccc ttcacaaaaa ccctgaaggt aaaatggtgg caaaaaacat cgaatggcat
    18001 aaagaaaacg gcacttatac tgcatcttat atcatcggtg caaccattgt gacggttggt
    18061 ttattgacgt tatccggtat ttggttcccg gtttccggta tggcgggggg cttactcacc
    18121 tttggtatgt cgattgttac actctctttt atgatcacca cccctgaaat ttgggtgccg
    18181 aatttaggtg gggatatgcc aaccccagcg catggtttcc catatctgtc agcagtcggt
    18241 cgtttaattg tgaaagatgt gattatgatg gcaggcggct tggttgccgc agcggaatgt
    18301 gcaaatcgca ttttagccag acgtaaagcg attgcttaat tgatagtggt aatgcaagcg
    18361 gtcattttct tgtaaaattt tgcaataaaa tgaccgcttg ttttttgtca atttgccata
    18421 atttcaagta taattcaatt acgtaatcgt ttgcttttat ggagaggaat aaaatgacaa
    18481 caatcggtac accgttaaga acgaacgcta caaaagtgat gatgttaggt tcgggcgagt
    18541 tggggaaaga agtggtaata gagctgcaac gcttaggcgt agaagtgatt gcggtggatc
    18601 gttatgacaa tgcacctgca cagcaagtgg ctcaccgtgc ttatacgatt tcgatgttag
    18661 acgggaatgc attgcgagat ttggtggaga aagaaaaacc ggattttatt gtgcccgaag
    18721 tagaagccat cgcaactgca accttggttg agttagagca agagggatat aacgtaatcc
    18781 caacggcaaa agcaacgcaa ttaacaatga atcgagaagg cattcgccga ttggcggcag
    18841 aagagttggg cttaaaaacg tcgccttatc gttttgtgga taattttgaa cagttccagc
    18901 aagcgattca agaaatcggc attccgtgtg tggtcaaacc gattatgtct tcttccggac
    18961 acgggcaatc agtaattaaa tctgaagcgg atattcaaca ggcttgggat tattcgcaac
    19021 agggcggacg tgccggcggt gggcgtgtga ttgtagaggg ttttatcaaa ttcgattatg
    19081 aaattaccca gcttaccgtt cgccatattc acggcacgtc gtttttagct cccatcgggc
    19141 atattcaggt tgatggcgat tatcgtgaat cttggcaacc gcagcaaatg tcggacattg
    19201 cattgaaaaa agctcaagaa accgcagaaa aaattacctc tgcactgggc ggaagaggga
    19261 ttttcggcgt ggaacttttt gtctgtggcg atgagattat ttttaacgaa gtctccccac
    19321 gtccacacga taccggaata gtaacaatgg catcacaaga gctttctcaa tttgctctgc
    19381 acgcccgtgc gattttgggc ttaccgattc cggaaattta ccgcatcagc ccggcagctt
    19441 ctaaagcgat tgtggttgaa gggaaatcgg ataatgtgcg ttttggcggt gtagataagg
    19501 tattagccga aatcgggacg aatatccgct tattcggtaa aggcgaagta aacggacacc
    19561 gccgattagg ggtaattctt gctcgtgatg aaaatacagt gagagcatta gaaaaagctc
    19621 ggcgagctta tgataagtta gatattcaat tataaagtca ggattttgat attcgttgat
    19681 agtaggcatt ttatgcctac tatcctatag gcctctaatc aaattggatg aaaaagttga
    19741 agattgttaa tcctctttgc cgagaatttg gttcacttcg ctcttataca atactgcttt
    19801 tgctccaaat acggcttgta caccgccacc gacttccagc acgtcaatcg caccgagttt
    19861 tttcagtgtt gctttatcca cggctttcac ctctttcact gccacacgca gacgggtaat
    19921 acaagcatcc acattttcga tattatttgc agagccaaga gcggcgataa ttttatgggc
    19981 attttcagtc agtgagctgg tttgttgttc gactacctct tcactctctt ccgcacgtcc
    20041 cggtgtcatt acgttgaatt ttttgatcag gaataggaac gagaagtagt aaagtgccgc
    20101 ccacggcaag ccgactatga tcactcgaag ccagttagtg ttctcgttgc cttgcaaaat
    20161 gccaaacagg gtgaagtcga tcacgccacc ggagaaggtg ttaccaatcg agatgttgag
    20221 taagtctgca atgtagaacg acacgccgtc taaaaaagcg tggaacacat agagccacgg
    20281 ggcaacaaac aggaacataa attcaatcgg ctcggtaatg ccggtgataa aagaggtgag
    20341 cgctgcacct aagaacagac cgctaatcgc accacggcgg gctttcggca cagcgtgata
    20401 catcgctaaa caggcggcag gtaagccaaa catcatcgta tcgaaacgtc cagcgaagaa
    20461 acgtgtacct tcggtaaata agccttgatg ggttggatcg gcgagttggg cgaagaagat
    20521 tttttgcgcc ccgataatgg tttcaccatt taccacttct gttccgccca attcggtgta
    20581 ccaaaacagc gggtagatca tatggtgtaa gccgaccgca ccgcttaacc gcattaagaa
    20641 accgtagaaa aaggtgccga tttcgcccat ttccgcaatc ccttgccctg ctgaaaccaa
    20701 ccactgttgg aaagccggcc agatcaaaaa gaacactgcc ccgacaaaaa tcgcagcaaa
    20761 ggcggtcaca atcggcacaa accgagagcc gccaaagaag ccgagaattt gcggcagctg
    20821 aatgttgtgg tatttgttat gcagtttcac cgtaatcaag cccattacca gcgagccgat
    20881 cacaccggta tcaatccctt tggtttcagg aggaaacagc ggaatgaggg cagaaatcgt
    20941 gcctgtcata atcaaataac cgaccactgc tgccagtgcc gctacgcctt tatctcgttt
    21001 tgctaatcca atgcctaaac cgatacacag taataatgca aggttgccaa acaccacact
    21061 gcctgccgat gccataatct ggaaaatgcc ctgtaaagtc gggttatcca gtatcgggta
    21121 ggcttgcacc gttgccttat tcgacaaagc tccgccaatc cctaacagca aaccggctgc
    21181 cggtaaaatc gcaatcggca gcataaaagc ttttccgact tgttgcagtt gtttgaacat
    21241 aaaatttctc cttttttatg gttgtatttg atggtttata gcatactgaa atttagccgt
    21301 atgaggtgtg atataactca cagatttgca aaatttttag caaattcgac cgcttgtaaa
    21361 aaaattaccg cttgcggaaa aattgaagta gaattaagca tttttttagc aaacacaaca
    21421 taaaaaattt ggagaagatt atgcctattt tacgttcagc aacttccact cagggtcgta
    21481 atatggcggg ggctcgtgca ttatggcgtg caaccggtat gaaagaaaat gattttggta
    21541 agccgattat tgcagtggtg aactcattca cccaatttgt gccggggcac gtccacttaa
    21601 aagatatggg gcagttggtt gcggcagaaa ttgaaaaagc cggtggggtg gcaaaagaat
    21661 tcaacaccat tgcggttgat gacggcattg caatgggaca cggcggaatg ctttattctt
    21721 taccaagccg tgatttaatt gcagacagtg tggaatatat ggtgaatgct cactgtgctg
    21781 atgcgatggt gtgtatctct aactgcgata aaatcacccc gggaatgtta atggcagcaa
    21841 tgcggttaaa tattccgact atttttgtct ctggtggccc gatggaagcc ggaaaaacta
    21901 aactttccga tcaattaatt cgcttggatt tagtcgatgc aatgatcgaa agtgccgacc
    21961 cgaatgtgtc tgatgaacgt attgatgcga ttgaacgtag tgcttgcccg acttgtggtt
    22021 cttgttcagg tatgttcacc gcaaactcaa tgaactgttt aactgaagct ttaggcttaa
    22081 gtttaccggg caatggttca atgttggcaa ctcatgctga ccgtaaagag cttttcctaa
    22141 gagcaggtcg tgaaattgtg gaactttgcc gtaaatatta ccaacaagac gatgagagcg
    22201 tgttacctcg ctcaatcgct accttcgatg catttgaaaa tgcaatgagc cttgatattg
    22261 caatgggtgg ttcaagtaat accgtgcttc atttactagc gactgctcaa gaagccggcg
    22321 ttgattttaa aatggaagat attgaccgcc tctctcgtgt cgtgccgtgc ttaagtaaaa
    22381 ttgccccgaa taccaataaa taccatatgg aagacgtaca ccgtgccggt gggattatgg
    22441 gcttgctggg cgaattagat cgtgcaggtt taatccatcg ccacgttaaa acagtgctag
    22501 gtttaacgat agaagagcaa ctgaatcaat acgatattat tcgtaataaa gatgaagatt
    22561 tacacaaatt cttccgtgca ggtccggcag gaattcgtac tactaaggct ttctcgcaag
    22621 attgccgttg ggacactgtt gatgatgacc gtgtaaacgg ctgtattcgc aatgtggaaa
    22681 atgctgtgac caaagagggc gggttagcgg tattatttgg gaatattgca gaagatggat
    22741 gtattgttaa aaccgcaggg gtagatgagt ctatttggaa attcactggc accgcaatcg
    22801 tgtttgaaag ccaagaagat gcggtagcag gtattttagg cggaaaagtg aaagaagggc
    22861 atattgtgat tatccgctat gaaggtccga aaggcggccc gggtatgcaa gaaatgcttt
    22921 atccaactag ttatttgaaa tcaatgggct taggcaaaaa atgtgcttta ttaactgacg
    22981 gtcgtttctc tggcggtacc tcagggttat ctatcggaca cgcttcaccg gaagccgctt
    23041 caggaggagc gattgcctta attaatgatg gcgatacggt ggcgattgat attccgaatc
    23101 gttcgattaa cttaatggtt tcagatgaag aacttgctgc tcgccgtgca gaaatggaag
    23161 cccgtggcga taaagcgtgg cagccgctaa aacgtgagcg agaagtgtcg tttgcactaa
    23221 aagtgtttgg acattttgcc acctctgctg ataaaggtgc ggtgagggat aaatcgctgt
    23281 taaagtaaat aaattagaaa aaagaagcat ttcaataata tattgatctg ctctttattc
    23341 agctgaatat ttaatctagc tgaatggaga gcagatcgat agcctaattt acatcttttt
    23401 gttttgatac agggaaatgt attacaagca ttttgccggt tttggcagac ctgcaatttt
    23461 agtggcttgt tttgcaggac catcaggaaa caagcgttgt aaataacggc tgttgccttt
    23521 ttcttcaccg aatttttgtg ccattgtctt taccagcatt cgaattgctg gtgaagtttt
    23581 gtattcttgg tagaaatccc gcaccagata aataatttcc caatgttctg cggttaagct
    23641 aatttgctct ttttcggcga ttttttctgc taattctaca gaccaatcat ttaaattagt
    23701 taaatagcct gaagcatcgg ttgggtattg gatattattt aattcaatcg tcatcaaata
    23761 aaaatgccac ataatgtggc attcctctta aactaaaacg ctagtcgtca ctacctagca
    23821 caccgaagat ttgtaagaag cttacaaata ggttgtagat agacacatat aaatcaacgg
    23881 ttgcacgaat gtagttggtt tcgccaccgt gaataatgtt gctggttgcc agtaaaatcg
    23941 caccgcttga gaaaatgaca aacaagctgc ttagggctaa acttaatgcc ggaattgcta
    24001 agaagatatt ggcaataata ccgactaata ataccacaaa cagggcaacc atcattccgc
    24061 tgataaagct catatctttt ttggtggtta atacataagc tgaacaagcg aagaaaacca
    24121 atgcggttgc accgaatgca agggcgacaa tatcccctaa acccgcaccg atgtaacggt
    24181 ttaaaatagg acctaaggta tagcctaaga agccggttaa agcaaatacg cttaagatac
    24241 ccgcactgct gtttgaggtg gcattcgtca agaaaagtaa gccgtaaaag ccgaccaata
    24301 atccccacca cggcatagcc ggtgcgttca tttgaattgc aacaaaagca acaactgcgg
    24361 agaaagtcag ggtcattgcc aataacatat aggtattgcg aagcactttg tgggtgctaa
    24421 ttagagattc accagttgaa tagctgtgag ctaaacgatt ttccataaaa attccttctg
    24481 tttttgtgtt taaatcggaa catccgatgg attaatatct atggataaat ttccataaat
    24541 caagaagtaa ggtagccgta atttattgta aagtcaatgc gattttgtaa cagaagagta
    24601 aaaaaaaaca tttagttgtg ttacacttaa taccactcta ctttatattc cttttcatcg
    24661 atgaattcgc tatgaagctc aatttcccaa cttatttaac cttatttcgg gtaggattaa
    24721 ccccactttt tattgtggct ttttatctgc cgacaaaata tgcgcctgaa atttcaacac
    24781 ttattttttt tattgcatcg attaccgatg cctttgacgg ctatttagca cgcaaatgga
    24841 atcaaaccac ccgacttggt gcatttttag atcctgttgc ggataaagtc ttggtagctg
    24901 ttgcgtttgt ctgcgtggcg gagtattacc atacttggtg gattactatt ccaatctgca
    24961 ttatgattgc ccgtgaaatt attatttcgg ctttgcgtga atggatggcg gagcttgggg
    25021 aaagggccag tgttgccgtt tcgatttggg ggaaagtaaa aacgactgct caaatgctcg
    25081 cattaggtgg aatgttatgg agacagaacc aactaatgga gattttagcc tttattctgc
    25141 tttatattgc agtgggttta acgatctggt ctatgatgca gtatttaaat gcttcaaaga
    25201 atagtctatt gaagtcataa gcggtcatat ttttattctt ttttgcaatt tgtagagtgg
    25261 aaatatcgct ctacaatttt caattttatc aactctaatg tctgaattta acgctaaatc
    25321 cttcctgtta aatgtgcctc accaaccggg tgtttatcgt atgtatgata gtaaaggcac
    25381 gataatctat gtgggtaaag ccaaagattt aaaaaagcgt ttagccagct attttcgtag
    25441 ccaactttcc agtaaaaaaa cggaagcctt ggtggcaaat cttgctaata ttgaaacgac
    25501 gatcactcat tctgaaacag aggcgttact ccttgaacat aattatatta aggaaaatca
    25561 gccgaaatat aatgtgttgc tcagagatga caaatcttat ccttatattc tgttaactaa
    25621 acataagcat cctcgtttaa cagcatttcg tggtagtaaa aagatcgccg gtgaatattt
    25681 tgggccttat ccgaatgtta cggcagttcg ggaaacactg aatttattgc aaaaaatttt
    25741 tcctattcgt cagtgtgaag atacttatta taaaaaccgt tcacgacctt gcttacagta
    25801 tcagatcgga cgctgtttag ccccttgtgt agagggctgt tattcacccg ttgaatacga
    25861 tcagcaggtt aatttggttc gtttgttttt acagggaaaa gatcaccaag tagtcgagca
    25921 tctggtacag aaaatggaag atgcggcgga ggctttggat tttgaaggtg cggcacgttt
    25981 tcgggatcaa attcaatcag taagagcggt gcaagaaaaa caattcgtct ctaatgatag
    26041 gctagatgat cttgatatta tctcgatcgt ttatcaaaat gggattgcct gtgtgcatat
    26101 cttatttgtt aggcaaggta aggtactagg caatcgttct tactttccaa aagtgccaag
    26161 taatacagat ttaaccgagc tcgcagatac ctttgtcggt cagttttatt tacaaatgaa
    26221 ccagcaccgc accataccta accaaattgt aattgatcat caattaagtg aatctaaggc
    26281 actcagtgaa attttaagtg agcaagcagg gcataaagtc agcattttag ataatgccat
    26341 cagaggtgat aaacagcgtt atttagcttt agcgagaact aatgcaatgg cagcattagt
    26401 tttacaactt aagcaagaat cccatattca aagccgttat caggcattga aagaaatgtt
    26461 gaaaatgtca gagattaaaa gaatggagtg ctttgacatc agtcatacaa tgggcaacca
    26521 aacagtggct tcttgcgtgg tgtttgatga aaatgggccg ctaaaatcgg attaccgccg
    26581 ttttaacatt gaggggatta ccggaggtga tgattatgca gcaatggagc aggctctctt
    26641 gaaacgctat aatcgagaat taccgccaga aaagatccct gatattattt ttattgatgg
    26701 tggcaaaggg cagttgaacc gagcgttaga tgtcttcgct aaacttgatg tggaatggga
    26761 taaatcgaaa ccgcttttaa ttggtgtggc aaaaggcgta gaacgtaaag ctgggctgga
    26821 aactttaatt atcagcaaat gggataaaga aattcactta ccgccagata gtcttgcctt
    26881 gcatttaatt cagcatattc gagatgaatc ccaccagcat gccattagtg ggcatcgtaa
    26941 aaaacgccaa aaggccttta ccgaaagtgg attagaatct attgccggtg tcggtgcaaa
    27001 acgccgacaa gcgttgctaa aatatttagg cggaatgcag ggggtgaagt ctgcaacctt
    27061 ggaagagatt caatcagttc cgggaatttc taaagcgttg gcagaggtta tttttgatac
    27121 attaaaaaat agttaaatta tcaatagcga tatcgcttcc agccgtaatg gtagcccact
    27181 cctatgcttg ggtaaattat agggtgaatt ggcagattta tgctttctga gcgtttatcg
    27241 ctttggttta atggctcatc atgttgcttt gtatttttat caacagtttg cttattgatt
    27301 actcgttgat ggtaatcctg aactgcttgc atatccattg gtactgtgct ttgttgttgg
    27361 ggtgtcgaac aggcggttag tgcaacgctc aatccgagta
//
LOCUS       DANLXQ010000015        27306 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.015, whole genome shotgun sequence.
ACCESSION   DANLXQ010000015 DANLXQ010000000
VERSION     DANLXQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27306
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.015"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            72..2681
                     /gene="adhE"
                     /locus_tag="PXD94_000500"
                     /gene_synonym="adhC"
     CDS             72..2681
                     /gene="adhE"
                     /locus_tag="PXD94_000500"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL6285395.1"
                     /translation="MAKNTQPFDAQAEVNELVEKGLKALDEFRKLNQEQVDFIVAKAS
                     VAALDKHGILAMHAYEETGRGVFEDKATKNLFACEYVVNNMRHLKTAGVISEDDVTGI
                     TEIADPVGVVCGITPTTNPTSTTIFKALIALKTRNPIVFAFHPSAQQSSAHAAQIVRD
                     AAVAAGAPENCIQWITQPSMEGTSALMKHPGIATILATGGNAMVEAAYSCGKPALGVG
                     AGNVPAYVEKTAKLKQAVYDIVMSKSFDNGMICASEQAAIVDKEIYAEFIKEMQSYGV
                     YLVNKKEKALLEKYIFGIDKAKDENCSGAKLNSAVVGKPAAWIAEQAGFKVPEKTNIL
                     LAECAFVGEAEPLTREKLSPVLALLKSDSTEHGLELSEAMVNFHGLGHSAAIHTENKA
                     LAKTFGERVKAIRVIWNSPSTFGGIGDVYNSFLPSLTLGCGSYGKNSVGNNVSAVNLI
                     NIKRVGRRRNNMQWFKVPSKIYFERDSIQYLKSMHDVHKVMIVTDRSMVDLGFVDRIT
                     EQLRQRRNQVMIQFFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMDAAKIMW
                     LFYEQPEVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTGSEVTPFAVITDG
                     DIKYPLADYSLTPTIAIVDPALVMSVPSHVAADTGLDVLTHATEAYTSILANDYTDGL
                     ALQAIKLVFENLEKSVKEFDETAREKMHNASTMAGMAFANAFLGICHSMAHKIGGKFH
                     TIHGRTNAILLPHVIRYNGTRPTKVATWPKYTNYVADVRFQDIARMLGLPAETPEQAV
                     EAYAQAVHDLAVRCGVKMSLREQGVDEQAFLNARRELALHAFEDQCTPANPRLAMIED
                     MEAIMTKAYYGKE"
     gene            2828..3724
                     /locus_tag="PXD94_000501"
     CDS             2828..3724
                     /locus_tag="PXD94_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL6285396.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSGNIATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            3734..5143
                     /locus_tag="PXD94_000502"
     CDS             3734..5143
                     /locus_tag="PXD94_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL6285397.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVSDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
     gene            5250..6473
                     /locus_tag="PXD94_000503"
     CDS             5250..6473
                     /locus_tag="PXD94_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HDL6285398.1"
                     /translation="MQTYQHKALCRVRTITLFLSLTKDKLQWETALNAAKAEFDLLVP
                     AIQTQGYHIQSIRIVTNSFGEYLDLTNIETAKQDLAYLKQLLTQLNDSGLRIRFAIGE
                     AKTPHELALLPELILNYGDLSNACVNIEPDEFGILDNETILQSAQIIKQIAEITPRGE
                     GNFNFTVNFNCKPFIPYFPASYHSSKLPNSFVIGLETPDLLVAVLKELKASPHNDFYR
                     QCYETMSQALQYHVDEVLEAVKSVQHKLQFAFAGIDSSAAPSKNCASMTEVYELMGVP
                     FFGAAGTVEASSLLTKVFKSIKHVPLVGFSGLMLAVTEDLGLAAGTHKAQFDIRALLT
                     YSAVCGIGLDTVPISNEATVEQIAALMRDTGTMAFRLNKPLTVRLFPIPNKSAGEVTE
                     FESDDLCNCRILAVP"
     gene            complement(6511..7707)
                     /locus_tag="PXD94_000504"
     CDS             complement(6511..7707)
                     /locus_tag="PXD94_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL6285399.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
     gene            complement(7727..8218)
                     /gene="tsaE"
                     /locus_tag="PXD94_000505"
     CDS             complement(7727..8218)
                     /gene="tsaE"
                     /locus_tag="PXD94_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL6285400.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILVNF
                     HQK"
     gene            complement(8218..8772)
                     /gene="orn"
                     /locus_tag="PXD94_000506"
     CDS             complement(8218..8772)
                     /gene="orn"
                     /locus_tag="PXD94_000506"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL6285401.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELARRWKPEILDKFSKGNTHLA
                     LDDIRESINELKFYREHFIQLDQK"
     gene            complement(8782..9261)
                     /gene="coaD"
                     /locus_tag="PXD94_000507"
     CDS             complement(8782..9261)
                     /gene="coaD"
                     /locus_tag="PXD94_000507"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL6285402.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(9263..10543)
                     /gene="waaA"
                     /locus_tag="PXD94_000508"
     CDS             complement(9263..10543)
                     /gene="waaA"
                     /locus_tag="PXD94_000508"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL6285403.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATLLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(10723..12102)
                     /gene="yegQ"
                     /locus_tag="PXD94_000509"
     CDS             complement(10723..12102)
                     /gene="yegQ"
                     /locus_tag="PXD94_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDL6285404.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYKMEEGKIDEVGQI
                     VPKYEPEIEVKNVAPTLGNGEATDKVFLYTESQRPDEQMTAFEDEHGTYFMNSKDLRA
                     VQHVERLTQMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDESLMDTLESL
                     AHRGYTEGFLRRHTHDEYQNYEYGYSISERQQFVGEFTGKRNAEGLAEVAVKNKFLVG
                     DSVEMMTPKGNIVFKIERMLNRKNENVDAALGDGHFVFLDVPADIELDFALLMRNLVN
                     TNTRNPHQA"
     gene            12230..12601
                     /locus_tag="PXD94_000510"
     CDS             12230..12601
                     /locus_tag="PXD94_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285405.1"
                     /translation="MKLKYLLATTLLAFSTFTAAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQASEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            complement(12640..16116)
                     /gene="dnaE"
                     /locus_tag="PXD94_000511"
     CDS             complement(12640..16116)
                     /gene="dnaE"
                     /locus_tag="PXD94_000511"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL6285406.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAAANNMVAMGLTDF
                     INFCGLVKFYGEALGAGIKPIIGADVYVRTESGNEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYVEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKLAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETIPLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRGSRIGIATIEDRSGKLDITLFS
                     EALENFGHLMQQDQIIVATGSVQFDDFSGGLKMSAREVLSLDEARGRFAKSLALAISK
                     EQLSADFIKKMTAIITPHKEGTLPLHFYYQSPEGRVLLKGGVEWRISPNEAMFSELKA
                     LLGENSVELEFE"
     gene            complement(16341..17438)
                     /gene="lptG"
                     /locus_tag="PXD94_000512"
     CDS             complement(16341..17438)
                     /gene="lptG"
                     /locus_tag="PXD94_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDL6285407.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASLSELVVMQASGFSRF
                     RIGLAVMKTALPLVIFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAKDGN
                     SFIYINHIKNEKSLDNVLIYQFGEGQNKRELQSVLKARTANYSDEKGWVLSKVEKSTV
                     GENQIQQAKQPDQVWQTSLTPSKLGIVSLKPESLSISGLADYVGFLKDTGQDPKRFEI
                     TFWRKVFQPISMAVMMLLAISFIFGPLRSSTMGAKIVIGIVAGFMFYVANIVFGNLSL
                     IATWLPIPVGALIPSLLCLIIVWWLLSKKRD"
     gene            complement(17438..18547)
                     /gene="lptF"
                     /locus_tag="PXD94_000513"
     CDS             complement(17438..18547)
                     /gene="lptF"
                     /locus_tag="PXD94_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL6285408.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            complement(18721..19518)
                     /locus_tag="PXD94_000514"
     CDS             complement(18721..19518)
                     /locus_tag="PXD94_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285409.1"
                     /translation="MRFWGKTFVVGLIGLGTQNTIFANDDAQISRYEQAKLRLSYDGN
                     VGNLLQQLSQRLKVGFIAYDVDITRKISLQNETETAIKTINEQIESQLTNIDVRFEKI
                     GERLFLVVSAKDVAPLLQNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIA
                     TNQEKIMAAKGKKAPQYKTVTKDNIGLKNIRVTALGTFLVFDTSVDAAKLRVKGNFED
                     IAQGENIIAILHQNTEAPPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
     gene            19756..21252
                     /gene="pepA"
                     /locus_tag="PXD94_000515"
     CDS             19756..21252
                     /gene="pepA"
                     /locus_tag="PXD94_000515"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL6285410.1"
                     /translation="MEFSVKNGSVEKQRTACLVVGVYEPRRLCSAAEQLDKLSNGYIS
                     TLLRRGDLEGKAGQTLLLHNVPNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINE
                     TGSMEAVSFLTELHVKGRTPYWNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFN
                     VAHRKDLPDAERALEHGKAVALGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAIS
                     TTIVDEKQMAELGMNAYLAVSRASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSG
                     GLSIKPAEAMDEMKYDMCGAATVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPG
                     DILTTMSGLTVEVLNTDAEGRLVLCDALSYVERFEPELVIDIATLTGACMIALGNHNS
                     GLMSPHNGLANELLAAAEQADDKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAG
                     QFLSNFTKKYTWAHLDIAGTAWKSGAAKGATGRPVSLLTQFLINKAQV"
     gene            21426..22373
                     /gene="cysK"
                     /locus_tag="PXD94_000516"
     CDS             21426..22373
                     /gene="cysK"
                     /locus_tag="PXD94_000516"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL6285411.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIAASDPDRYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSTLLFEGIEA"
     gene            22559..23821
                     /gene="rho"
                     /locus_tag="PXD94_000517"
     CDS             22559..23821
                     /gene="rho"
                     /locus_tag="PXD94_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL6285412.1"
                     /translation="MHLTQLKNTPVSKLVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
     gene            complement(23882..25000)
                     /locus_tag="PXD94_000518"
     CDS             complement(23882..25000)
                     /locus_tag="PXD94_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL6285413.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDIHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(25013..27160)
                     /locus_tag="PXD94_000519"
     CDS             complement(25013..27160)
                     /locus_tag="PXD94_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6285414.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAEKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGIQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
BASE COUNT         7905 a         6095 c         5337 g         7969 t
ORIGIN      
        1 ataactcttt tgggtaataa tttcatcagt taaaaaaatt atcaaattta aattttatga
       61 ggaaccaaac tatggctaaa aatacgcaac catttgatgc ccaagcagaa gtgaatgagc
      121 ttgttgaaaa aggcttaaaa gcccttgatg agtttagaaa actcaaccaa gaacaagtgg
      181 attttattgt cgctaaagcc tctgttgctg cccttgataa acacggtatt ttagcaatgc
      241 acgcttacga agaaaccgga cggggtgtgt ttgaagacaa agccactaaa aacctgttcg
      301 cctgtgagta tgttgtcaat aatatgcgac atttgaaaac cgcaggggtc attagtgaag
      361 atgatgtcac cggtattacc gaaattgccg accctgtcgg tgttgtctgc ggtatcaccc
      421 caaccaccaa cccaacctca accaccattt tcaaagcgtt aatcgccctt aaaacccgta
      481 accctatcgt ttttgctttc cacccgtctg ctcaacaatc ttctgcccac gctgctcaaa
      541 ttgtgcgtga tgctgccgtg gctgccggtg caccggaaaa ctgtattcaa tggattaccc
      601 agccttcaat ggaaggtaca tctgctttaa tgaaacaccc gggcattgca actattttag
      661 ccaccggcgg taatgcaatg gttgaagccg cttactcttg cggcaaaccg gcattaggag
      721 ttggtgcagg caacgtaccg gcttatgtag aaaaaaccgc taaattaaaa caagcggtgt
      781 atgacattgt aatgtcaaaa tctttcgaca acggtatgat ttgtgcctct gaacaagcgg
      841 cgattgtaga taaagaaatt tacgctgagt ttattaaaga gatgcaatct tacggcgtct
      901 atttagtgaa caaaaaagag aaagccttat tggaaaaata cattttcggt attgataaag
      961 ccaaagatga aaactgctca ggtgcaaaac tcaattcagc ggtggttggc aaaccggctg
     1021 cgtggattgc agaacaagcc ggctttaaag taccggaaaa aaccaatatt ctgttagccg
     1081 aatgtgcctt tgtgggcgaa gctgagccac ttacccgtga aaaactttcg cctgtactgg
     1141 ctctgttaaa atccgactcc accgaacacg gtttggaatt gtctgaagcg atggtgaatt
     1201 tccacggttt agggcatagt gccgccattc ataccgaaaa caaagcctta gccaaaacct
     1261 ttggcgaacg ggtaaaagcc attcgggtga tttggaactc accatcaacc tttggcggta
     1321 tcggtgatgt atataactcc ttcctgccat cgcttacgct gggctgtggt tcctacggta
     1381 aaaactctgt aggtaacaac gttagtgcgg tgaatttaat caatatcaaa cgtgtgggca
     1441 gacggagaaa taatatgcaa tggtttaaag tgccatcaaa aatctacttt gagcgtgact
     1501 caattcaata tttgaaatca atgcacgatg tgcataaagt aatgatcgta accgaccgct
     1561 caatggttga tctcggcttt gtggatagaa ttaccgaaca acttcgccaa cgtcgcaacc
     1621 aagtgatgat tcagttcttt accgatgttg aaccaaaccc aagcctgcaa accgtacaac
     1681 gtggtacaga attaatgcgt agcttccagc ctgatactat catcgcactt ggtggtggtt
     1741 caccaatgga tgcagccaaa attatgtggt tattctacga acaaccggaa gtcgatttcc
     1801 gtgatttagt gcaaaaattt atggatatcc gcaaacgtgc attcaaattc ccacaattag
     1861 gcagaaaagc gaaatttgtc ggcattccaa ccacctccgg aacaggctct gaagtcacac
     1921 cgtttgcagt aattaccgat ggcgatatta aatatccgct cgctgactac tcgcttaccc
     1981 caaccattgc gattgtcgat cctgcgttag taatgtctgt gccgtctcac gttgctgccg
     2041 acaccggttt agacgtatta actcacgcta ccgaggctta cacatcgatt ttagccaacg
     2101 actataccga cggcttagcc ttgcaagcca ttaagttagt gtttgaaaac ttggaaaaat
     2161 cggtcaaaga gtttgatgaa accgcccgtg aaaaaatgca caatgcttcc acaatggcag
     2221 ggatggcatt tgccaatgcc ttcttaggta tttgccactc aatggcacac aaaatcgggg
     2281 gtaaattcca caccatccac ggtcgaacca atgcgatttt actgccacac gttattcgct
     2341 ataacggcac acgcccaacc aaagtggcaa cttggccaaa atacaccaat tatgtcgcag
     2401 atgtgcgttt ccaagatatt gccagaatgc ttggcttacc ggcagaaacc ccggaacaag
     2461 cggtggaagc ttacgcccaa gcggtacacg atttagccgt tcgttgcggc gtgaaaatgt
     2521 cgcttcgtga gcaaggcgtt gatgagcaag ccttcttgaa tgcccgccgt gaacttgccc
     2581 tacacgcatt tgaagaccaa tgcacaccag caaacccacg tttggcaatg attgaagata
     2641 tggaagcgat tatgactaaa gcctactatg gcaaggaata aagcacaatt taatcgttaa
     2701 acttactaaa tggccgcttg caagcggtca tttttcttga aatttttact tttcccttca
     2761 caaaatactt tcgctctttt acaaagcata gtatttttat cgctcctttt atcttacagg
     2821 aaatactatg caaattacga ttttagacgg cggaattagc cgtgaattaa tgcgtcgcaa
     2881 tgctccattt catcagcctg aatggtctgc tgccgctttg tatgaaggcc cacattttgt
     2941 gcaagcggta cacgaagatt ttatcgctca cggggcagaa gtgattacta ccgacagcta
     3001 tgcggttgtg ccgtttcaca ttggcgaaaa acgctttgcg gcagacggca aaatgcttgc
     3061 cgatttagcc ggtcgccttg ccaaacaagc ggtcaaaaat tcaggaaata ttgcaactca
     3121 aattgcaggc tcacttccac caatgtttgg ctcttaccgt gccgatttaa ttcaaatgga
     3181 acgctttgct gaagtcgctc agcctttaat tgacgggctt tcgccttatg tggatatttg
     3241 gctttgcgaa acccaaagtg cgattattga gccgatttcc atcaaagcat tattgccgaa
     3301 agacaaccgc ccgctttggg tctcttttac cctcaccgat gatgaaccaa ccaccgagcc
     3361 acaacttcgc tcgggcgaaa gcgttaaaca agcagtcgaa gaaatggtga agttaggcgt
     3421 gcaagcgatt ttatttaact gctgtcagcc tgaagtgatc ggcgaggcat taagtgtaac
     3481 ccaaaaaact ttagccgaat taaaagctac ccatattcgc acaggagctt atgccaacgc
     3541 ctttgctcca caaccaaaag atgccaccgc caatgacggt ttagatgaag ttcgccaaga
     3601 tttaaccctg gaggcttatt taggctgggc aaaaaaatgg acggaacaag gggcaactat
     3661 tatcggtggc tgctgcggca ttggggttga gtatattgaa acgctggcaa aaaatttaaa
     3721 ataaggaaat cgaatgtcaa ataaaaaaat aggattaatt tcactcaccg ccttagtgct
     3781 aagctcaatg attggctcgg ggattttcag cttaccgcaa aatatggcag ccgttgccgg
     3841 agcagaagcc attaccattg gctggctgat taccggtgtc ggcattatct ttttaggttt
     3901 atcgtttttc tttatttccc gcttgcgtcc ggatttagac ggtggcattt acacctacgc
     3961 acgggagggt tttggcgagc tgatgggctt tatgtcagcg tggggctatt ggctctgtgc
     4021 caccattggg attgtaggat acctcaccgt tgcttttgaa ggtttgggtg tatttaccga
     4081 caacccgaac accgtgattt tcgggcaagg taacaccctc gcctctttta ttggatcttc
     4141 aatcattgtg tggctggttc acgccctcat cgcaggcggt atcaaagaag ctgcctttgt
     4201 aaacttaatt gccacttttg tgaaagtcgc tccgctcatt ctgtttatcg ggttaggcat
     4261 ttggtatttc gatgtggatc tgtttaatag cgatgttaaa gccaccgcac ttaataatag
     4321 tgtaagcgac caagtgaaaa gcacaatgtt aatcacctta tgggtattta ccggtgtgga
     4381 aggggcttct gtgctgtcgg ctcacgcgaa aaagcgttct gacgtgggac tggcaaccgt
     4441 attaggtatt attattgcac ttgctcttta tgtggcaatt accattcttt cactcggaat
     4501 tctgccacgg gaaactattg ccaatatggc aaatccgtca atggggccgt tgctcgatgc
     4561 aatgatgggc ccaaccggca aagtgattat tactgcttgt ttaattgtat cggtgcttgc
     4621 ctcttacatt agctggacga tgtattcggc agaagtgcct tatcgtggct caaaaaacgg
     4681 ggctttcccg aaaattttag acaaagtgaa taaaaacggc acgccgatta actcactctg
     4741 gtttaccggc tttatcgtgc aattttgctt ggtgttggtg tttgtttttg agcaaagcta
     4801 taacacctta ttgctgattt ccacctcgat gattttaatt ccctatttct taatcggggc
     4861 ttatttattc aaattagcga ttcaaaccaa cgccacttgg tacattaaac tcaccggctt
     4921 tatggcagcc ctttacggat tatggattgt ctatgccgca ggactagaat atttgctgct
     4981 ttcggtggtg ctttatgtac ccggaatttt actcttcctc tactccaact ataaattcaa
     5041 cggcagatta accctttcaa ccgctgaaaa ggtcatctta atcgtgctac tggtaatttt
     5101 tggctatgcc gtgattgagc tgccagcact attagcagca taattcccat caaaaatgac
     5161 cgcttgcaag cggttatttt tctaggatct tctgcaaaga ttcattagaa tagaaacaac
     5221 gtcaataata aataaaaaga ggagaaacaa tgcaaactta tcaacataaa gccctttgtc
     5281 gggtaagaac cattaccctt tttctgagtt taaccaaaga taaattgcag tgggaaacgg
     5341 cattaaatgc ggcaaaagca gaatttgatc tattagttcc cgctattcaa acacaaggct
     5401 atcacataca atcgattcga atcgttacca acagtttcgg ggaatatctt gatctcacta
     5461 atattgaaac agcgaaacag gatttagcct atcttaaaca gctattaacc caacttaacg
     5521 atagcggttt acgcattcgc tttgccattg gtgaggcaaa aacaccccac gaattagcat
     5581 tactacctga gctgatttta aattatggtg atttatctaa cgcttgtgtc aatattgaac
     5641 ccgatgaatt tgggatttta gacaatgaaa ccattttaca atctgctcaa attatcaaac
     5701 aaatcgctga aattacccca agaggtgaag gcaattttaa ttttacggtg aattttaact
     5761 gtaaaccttt tattccttat tttccggcaa gctaccattc aagcaagctc cctaacagtt
     5821 ttgtgatagg cctagaaaca ccagatctat tagtagcggt gttaaaagag ctgaaagcca
     5881 gcccacataa cgacttttat cgccaatgct atgaaacgat gtctcaagcc ttgcaatatc
     5941 acgttgatga agttctcgaa gcagtaaaat cggtacaaca caaattacaa tttgcctttg
     6001 ctggcattga tagttctgct gctccgtcaa aaaactgtgc ctcaatgaca gaagtttatg
     6061 aattaatggg cgtaccattt tttggggcag ccggaacagt agaagcctct tcacttctga
     6121 caaaagtctt taaatctatc aaacatgtac cgcttgtcgg tttttcaggt ttaatgttgg
     6181 cagtaaccga agatttaggc ttagccgcag gcacacacaa agcccaattc gacattcgtg
     6241 ctttactaac ttatagtgct gtttgtggca ttgggttaga taccgtaccg atttccaatg
     6301 aagccactgt tgaacaaatt gccgcattaa tgcgagacac cggcacaatg gcatttagac
     6361 taaataaacc gcttacagta cgtttatttc caattccgaa taagagtgct ggggaagtga
     6421 cagaatttga aagtgatgat ttatgtaact gccgaatttt agctgtacct taaaataaag
     6481 gctgatggat atttccatca gcctttcaat ttacttcacc ttcaattttt tacctaccgt
     6541 gatctgataa tttttcaaat tattcagctc actgattttt tgcggcgtag taccgtaagc
     6601 tctagcaatc gagtaaaccg tttcatcttg ttggacaata tggtagccac tgttagacac
     6661 cttaatttct ctttgagagt ctgatttttt agactgattt ttttgatctt tctcaagagc
     6721 tgttttttct tttagttgct gctctttttt cttattggtt ttagccgaat cttctttttt
     6781 atcctctttt ttgctctttt ctttcaattt tgtcttatct gattgcgtat tcttctcttg
     6841 tttctcagaa gcaggctttt tagttaaatc agctttagca gttttttcct ctttcttatt
     6901 ttgcttatct tctttgtccg attttttact gaccgaagaa ctagcaactg cattttttgc
     6961 ccgataagcg actaatccat tataaatggc ccgagcaact tgtttacgat aatttgcact
     7021 ggctagcttt gcttcctcgg ttgaattaga taaaaatccc gtttccacca atacagatgg
     7081 aatatcaggt gaacgcagaa cacttaagct tgcgtgctga ggagacgatt ttgctaatgc
     7141 agccacattt gacattttag ctaaaatgct cttgcctaat tcatagccgg cacgttgaga
     7201 gtgagagaac tgtaaatcaa gcactgtttg atttaaataa cgttcattgt tgcttgaaag
     7261 caccgaacca gccccgccaa gtaattccga ctgcttctcg tggtcttcca accatttacc
     7321 catttcatcg ctagcacggc tattagaaag tacccaaacc gaagcccctt tcaaggaatc
     7381 gccacgaggc gaagagtctg cgtgaataga aaccagcaga tcggcacgat gcttacgggc
     7441 aatctcggta cgttgcggta attggataaa ataatctccc gagcgtgtca tcactgcttt
     7501 aaaattagga tcggcatcta acaaggcttt tagctcttta gaaattgcga gggtaacgct
     7561 tttttccaaa atccccagat ttttaccaat agcccccgga tcttttcccc cgtgtccggc
     7621 atcaatcgcc acaatggttt tggcaaatgt ggaaaaactg aataaactca atgtgctaaa
     7681 aacaataaat ctgataactt gcttcattac ctcccctaca agcggtctat ttttgatgaa
     7741 aatttactaa tatatctact gcagtttgat tttgcggcaa caggctaatc tgccgtccgt
     7801 cattcttata atcaatttgg atcacaaaat cagcctctgg aatcattcct ttacctttaa
     7861 ctgcccattc aagcaaacaa agcgtttgtg gtttgaaata atcacgaatt cccataaact
     7921 ccagctcttc aggatcggct aagcgataca aatcaaaatg gtaaaggctg aaaggcggaa
     7981 gttggtattc ttccaccaga gtataagttg ggctttttac attgccttga tgcccgaaag
     8041 cccgtacgat cgagcgggtt aaggtggttt tacctgcccc aagttcgccg tttaggtaaa
     8101 caaccagcga atgagcttgg tcttgcaata aatatttctt aagttctaac gcgaatgatt
     8161 gaccaaattc cagcaattta gtttcatctt cgcagtaaaa agagagagaa tctgtcatta
     8221 tttttggtct agctgaataa aatgttcccg ataaaatttc aattcgttaa tggattcacg
     8281 aatatcgtcc aacgctaaat gcgtattgcc tttactgaat ttatctaaaa tctccggttt
     8341 ccaacggcgt gccagctctt ttaaagtact cacatctaag tggcgataat ggaaataatc
     8401 ggctaaatca ggcatatatt tatacaaaaa acgtttatct tgagcaatgc tgttgccgca
     8461 aatcggagaa gcgccttttg gcacccagcg ttttaagaag tctaaagttt gcaactctgc
     8521 tgcccgttca gtcaatttgc tcgctttcac tcgttcaatc agcccgttag cggtatgggt
     8581 tttgacgcac caatcactca ttttctctaa taaatcatcg gtttggtgta ccgcaagtac
     8641 cggtccttcc gctaaaatat ttaaatcctt atcggtcaca atggtggcga tttcaataat
     8701 gcgttctttt tccggatcta accctgtcat ttcaagatcg atccaaatta aattttgttc
     8761 gttttgagcc attttatttc cttacacttt tgcaattttt agggcatttt tgaccgcttg
     8821 cggcacaaat tggctgatgt caccattgtg gcgatagatt tcccttacca tcgttgaaga
     8881 aaggtaacgc cattctattg aagggggaaa aaaaatagtt tctaaatcgc ccgataattt
     8941 gtaatttaac tgagcgagtt ggatttcata gtcaatatca tctgccccac gaataccacg
     9001 gattaacgct ttggcattct gctgtttggc aaagtctgct aacaatccac taaagccaat
     9061 cacctcaata ttttgccact gggtacagct ttgtttaaca agctccactc gttcttcaag
     9121 actaaaaagc ggctgcttgc ttgggttttg agcaacggca acaatcactt ttccaaataa
     9181 gtgagaggct ttgcaaatta tgtccaaatg cccattggta atgggatcaa aggtgcctgc
     9241 ataaattacg ttataactca ttttatttct ctaaatagga ggctaataaa tccaaatggc
     9301 gttttaatgc cccttgattt tcctgtaaca cgcttaagcc ggattttgca atttcttgtc
     9361 cgatttgggg ctgcttaagc aataattgaa tgctctcggt caaagcctcg actgaacttt
     9421 caatttcaat cacgcctttc acctcacgca atttctcaaa gatttcaggg aaattatagg
     9481 tgtaaagccc agaaaccacc ggaatattaa aagcgattgg ttcaagtgga ttatgcccac
     9541 cgtgtttgac caaactgcct cctacaaaag caatgtcaga caacccatac agtaacatca
     9601 tttcccccat tgtatcacct aacattactt gagtattttg gcttaacgtt ttattctcag
     9661 tacgttttac ataatttaaa tctgattttt gaatcagtag ctcaactaaa ccaaaacgct
     9721 ccggatggcg aggcactaaa atcaacacta aattcgggta atgctctaat aagagtttat
     9781 gagcatctaa aatcagcttc tcttcccctt cgtgagtact gccggcaatc caaatcggtc
     9841 tctctgttaa attcagacta gctttagttt taatcaccga ttgatgaagc tgtggcgtaa
     9901 tttctaaatc aaatttcagg ttaccggtat taaccatttt tgccggtgca taaccgagtg
     9961 ctaaatagcg atctcggctt acctcatctt gagccataat cagactaatt tggttgagca
    10021 tatccttaat gctacctttt acccagccat aacgcttcgc agaacgtggc gataagcggg
    10081 cattggcaat cacaaatgga attttacgaa tatttacttt acgaattaaa ttcggccaaa
    10141 gttcggtttc aattacaatc atcaatttcg gatcgataaa attcaaaaac cgctcgatag
    10201 catcaggtaa gtcataaggc aaataaaaat gtgaaacact attaccaaaa gcagcttgca
    10261 cacgcccaga gcctgttggc gttaccgttg tgactatcaa cggaagttca ggatattgct
    10321 tgctgactgc cttaatcaaa agcgttgcag caatcacctc accaaccgat gccgcatgga
    10381 ttaccacccc ttttgctttc ggtttttccg attcgtcata aatcccataa cgctcccata
    10441 aacgtttccg ataagccggt tgtttacgcc ctttgtacca cattaatagc agaaccacag
    10501 gctgtaatag ataacttaaa caaatataga gtaaacgtag catttatttt ccttatacca
    10561 taaccctcac ctcttgtggg agagagtcaa atacgccaat aaatcgttcc tctcttgttc
    10621 gagagggggg aacaagcggt tatttttacc aaaaattttg caatctatat cctcaaagac
    10681 aaaaccacgg aataacctta atccgtggtt tatctcgcat cattacgctt gatgtggatt
    10741 gcgggtattc gtattcacta agttacgcat taaaagtgca aaatccaact caatatctgc
    10801 cggcacatct aaaaagacaa aatgcccgtc accgagtgct gcgtccacat tttcattttt
    10861 gcggttaagc atacgctcga ttttaaatac aatgttgcct ttaggggtca tcatttccac
    10921 tgaatcgccc actaagaatt tgtttttcac tgccacttcc gccaagcctt ctgcattgcg
    10981 tttgccggta aattcgccca caaattgttg gcgttctgaa atcgaatagc catattcata
    11041 gttttggtat tcatcgtgag tatgacgacg taagaagccc tctgtataac cacggtgtgc
    11101 aaggctttca agagtatcca ttaaactttc gtcaaacggt ttaccggcag cggcatcatc
    11161 aatcgcctta cgatacacct gagccgttct cgcacagtaa tagaacgatt tcgtacgccc
    11221 ctcaattttc agtgaatgca cccccatttg agttaaacgc tcaacgtgtt gtactgcacg
    11281 cagatcttta gagttcataa aataggtgcc gtgttcatct tcaaaggcgg tcatttgctc
    11341 atcagggcgt tgtgattctg tataaaggaa cactttatct gttgcttcgc cattacctag
    11401 agtaggagcc acatttttga cttcaatttc cggctcatac ttaggcacaa tttgccctac
    11461 ctcatcaatc ttaccttctt ccattttata ttcccaacgg caagcattgg tgcaagtgcc
    11521 ttggtttggg tcacgcttat tgatatagcc tgaaagcaag cagcgacctg aatatgccat
    11581 acacaaagca ccgtgaacga agatctcaag ctccatatcc ggcacttgtt cacgaatttc
    11641 ttcgatctct tcgagtgata attcacggga taaaatcaca cgggttagcc ccatttgacg
    11701 ccagaatttc accgttgccc agtttaccgc attggcttgc accgaaagat gaatatccat
    11761 ttcagggaaa ttttcacgca ccaacataat taagcccgga tcagacataa tcaaagcgtc
    11821 cggcttcata tccactacca cttttagatc tttgatgaag gtttttaatt ttgagttgtg
    11881 tggcgcaatg tttaccacca catagaattt ttttcctaag gcgtgggctt catcaatcgc
    11941 aacttttaaa ttggcgtggt taaattcatt attacgcaca cgcaagctat aacggggctg
    12001 tcctgcataa atcgcatcgg caccataagc aaaagcgtaa cgcatatttt ttaaagaacc
    12061 tgcaggcgaa agtagctcag gtttggcata ttttaaagtc atattattct cttttctgat
    12121 aacaagtcag gacttctcga cgtgagaagc gtaagcggta tattttctaa gaaatattgc
    12181 gtgatggcaa gctaatccat tacaatccga tttttatgtg gggaacaata tgaaacttaa
    12241 atacctttta gcaacaacat tactggcttt ttcaacattt accgccgctc aaagcatcaa
    12301 gacggagatg atgcaaatga atatgcaagc gaataaattg atgcgtgccg aatcggcaga
    12361 agagtttagc gaaattgcga atgatttcat tataattacc gagaaaacca aagggatttt
    12421 tcccaatagc gtagaaggcg ataaagcagc tcaggaagat taccaagccg gtatgcaaaa
    12481 attaattgat atggttaatc aagccagtga gaaagctcaa gcaggtgaat tgcaagaagc
    12541 aaaaatgctg atttccgagc ttgaaatgat caagcgtgaa tatcacaaaa aatacaaata
    12601 attcaacaca ttaaaagggt gagctaaagc tcaccctttc tattcaaatt ccagttcaac
    12661 cgaattctca ccaagaagtg cctttaattc gctaaacatt gcctcattcg ggctgattcg
    12721 ccactctact ccgcctttga gcaataccct accttccggg ctttggtaat aaaaatgcag
    12781 cggtaaagtg ccttctttat gtggggtgat gatagcggtc atttttttaa taaaatctgc
    12841 agaaagttgc tctttgctaa tcgctaacgc taaacttttg gcaaatcgac cccttgcttc
    12901 atcaagggat aacacttctc gagccgacat tttcagtcca ccgctgaaat catcaaactg
    12961 taccgaacct gttgccacaa taatttggtc ttgttgcatc aaatgcccga aattttctaa
    13021 ggcttccgag aaaagggtaa tgtccaattt ccccgagcga tcttcaatag tcgcaatccc
    13081 aatacggctt cctcgcttgg taactgccac tcgagatgcc ataacaattc ctgaaaccgt
    13141 ggtcatttgc cctcggaaag tcggttgcaa ttcattcagc cgattagggg catagtggga
    13201 taattctttg aggaatctac caatcgggtg tccgcttaaa tacagcccga gcgtggcacg
    13261 ctctccatct aaaatcactt gctccgacca ttttggcgta ttggcgtaag cattttgcac
    13321 ttcttccggt gtctcggtaa gcacgccaaa catatcgctc tgcccgagtt cctccatttt
    13381 agcgtgctga tctgaggctt taagtgcatc ttccaaattc ttcatcaagg cagctcggtg
    13441 agggcctaat ttgtcaaaag caccggacat aatcaggctt tcaaaggtgc ggcggttgat
    13501 ttttttcaga tcgacccgag cggttaaatc aaatagatct ttaaatttcc cctctttttg
    13561 gcgagctgcc agaattgcct caatcggtcc ttccccaacg cctttaattg ctcctagccc
    13621 gtaaacaatt tcgccttttt catttacgct aaaacgatgt ttaccgctgt ttacatcagg
    13681 cggcacaacg gttaagccca tattcataca ctcatcgtag aacccgacaa ttttatcggt
    13741 attatccatc tctgaggtca ttaccgcagc cataaattct gccggataat gggcttttag
    13801 ccaaagtgtt tggtaagaaa ccaacgcata agctgctgag tgagatttat taaagccgta
    13861 gccggcaaac ttttctacca agtcaaaaat ttgaccggca agtttaccgt caatcccttt
    13921 ggcttccgcc cctttttcaa acacttcacg ctgtgcagcc atttcctccg gtttcttctt
    13981 acccattgca cggcggagta agtccgctcc accgagtgta taaccggcaa gctcttgtgc
    14041 aatttgcata acctgttctt ggtaaacaat gaccccataa gtcggctcga gaataggttt
    14101 taagcattcg tgttggtatt gcggatcagg ataagaaacc tcttctttgc cgtgtttacg
    14161 gtcaataaag ttctgcacca tacctgattc cagcggccct gggcggaata gtgccaccaa
    14221 tgcgataata tcttcaaaac agtccggctt caggcgagaa attaagtctt tcattccccg
    14281 tgattcaagc tggaatactg ctgtagtttt agctgccagc aacagagcaa aggatttttt
    14341 atcatcaagc ggaatggttt caatccgaat cggctctttg ccttcacgag ccaaacgttc
    14401 gttaatcatc tccaacgccc atttgatgat ggtgagcgta cgcaagccta agaagtcaaa
    14461 cttcaccaat cctgcatatt ccacgtcatt tttatcaaaa tgggtaaccg ggtgtaagcc
    14521 ctcgctatcg caataaagcg gcgaaaaatc ggtaatcgca gttggggcga tcaccacacc
    14581 accagcgtgt ttacctgcat tacgagttac accttcgagc ttacgagcca tatcaataag
    14641 gtctttcacc tcttcatcgg cttcatatag ctcaggcaat tttggctcag cggcaaaggc
    14701 tttttcaagc gtcattcctg gatcgggcgg aatgagtttg gagatgcgat ccacaaagct
    14761 ataggggtgt ccaagcactc gccctacatc tcgaatgacc gctttcgccg ccatagtacc
    14821 gaatgtgata atttgcgata ccgcttgtcg cccgtaggtt tccgcaacgt gttcaatcac
    14881 acggtctcgc ccgtccatac agaaatcaac gtcaaaatcg ggcattgaaa cacgctcagg
    14941 gttgagaaaa cgctcaaaaa gcaaatcaaa ttccaatggg tcaagatcgg taatcttcaa
    15001 cgcataagcc accaatgagc ctgcccctga gccacgaccc ggtcctaccg gaatatcatt
    15061 atctttggac cattggataa actccatcac gatcaaaaag taacccggga accccatttg
    15121 gttgatcaca tctaattcaa cttgcaatct ttcgtcataa accgaccgct tgttcgccct
    15181 ttcctcagga tcagggaata gaaactctaa acgctcttct aagccttctt tcgatttttt
    15241 taccaagaaa tcttcggtgg aaagctcacc ggtcgggaaa tttggcagga aatactcgcc
    15301 caaacgcacg gtaaccgaac agcgtttcgc aatttcaacc gtgtttgcca atgcactcgg
    15361 taaatcggca aacagcttgc acatttcctc ttcagtacgg aaatattgtt tcggtgagta
    15421 ttttttcggg cgtttcgggt cgtctagggt gtagctgtcg tgaatggcaa cccgaatttc
    15481 gtgtgcgtca aagtcatctt ctttcaagaa gacaacatca tttaccgcca ctaacggaat
    15541 tgcatttttt tgtgaaaatt cgaccgcttg tttgatatag cgttcttcgt ctgcccgtcc
    15601 ggtacggcaa acggaaaggt aaaaatgatt cgggtaatat tgctgataaa aagctaattt
    15661 ttcttcagct tcttgtcggt tttctttgag taaggcttta cccacatcgc ccattcgccc
    15721 gccggaaaga atgatgatgc cctcattcag ctctgctaac cattcacgtt ccaccatcgg
    15781 aaattccaca tagccttgct gataggcttt ggacaaaatt tgggtaatat tatggtagcc
    15841 tttgttgttc ttcgccagta atgtcagctc aaaataatct tcattgccgc tctcggttct
    15901 aacataaaca tctgccccga taatcggctt aatccctgct cccaatgctt cgccatagaa
    15961 tttcaccaac ccgcaaaagt ttataaaatc cgttaagccc attgccacca tattattagc
    16021 ggcagcggtt ttaactaagg attttacctt agctaaacca ttaatcattg agaaatcact
    16081 gtgaactttt aaatgaacga aacgacaatc tgacatagga atataatcta agtaaagaag
    16141 tttcccctct ttagtaaaga ctgtcaggtt ctaaaaagtg aagccttctg tgaataatcc
    16201 cgttgggcga atgttattcg cccctacaaa cgagcattat tcttgctcag agttacattt
    16261 aaaatccgaa tgtaggggcg aattacattc gccctttcag aacctaacaa ccctgcttta
    16321 tctaaagaag ggagttaaaa ttaatcacgt tttttgctta acagccacca aacgatgatc
    16381 aaacagagta ggcttggaat taatgctcca accggtatag gtaaccaagt ggcaattaag
    16441 cttaaattgc caaacacaat atttgccaca tagaacataa agccggcaac aataccaatc
    16501 acaattttcg cccccatcgt actactacga agcggcccga aaataaagga gatcgcaagt
    16561 agcatcatca ccgccattga gattggttgg aatacttttc gccagaaggt gatctcaaaa
    16621 cgtttcggat cttgtccggt atcttttaag aagccgacat aatctgccaa gcctgaaatc
    16681 gagagcgact ccggtttgag tgaaacaatg cctaatttgc taggggttaa actggtttgc
    16741 caaacttgat ctggctgttt agcttgctgg atctgatttt ctcccacagt cgatttttcc
    16801 actttagata gcacccagcc tttttcatca ctgtaattcg cagttcgggc ttttaaaaca
    16861 gattgcaatt cacgtttgtt ttgtccttcg ccgaattgat aaatcagtac gttatctaag
    16921 cttttttcat ttttaatatg attaatatag ataaatgaat tgccatcttt cgcccaaaac
    16981 ccgtcttttg tggcaagcat tgagccgcca ctttgggcaa tcgaacgcat attacgggca
    17041 aactgctcgg tttgtggcac gccccattcg ccgataatca tcgtaaaaat gactaacggt
    17101 aaggcagttt tcatcacggc taaaccaatg cggaaacggg aaaaaccgga ggcttgcatc
    17161 accaccaatt cactaaggct cgctaggttg cctaaaccga gtaatgagcc aagtaatgcc
    17221 gcaatcggga aaaaggtttc gatatctcgt ggaatagtta aaaaagtata gtatgccgct
    17281 tttaagccat cgtaagatcc acgtcctaca tcacgaaact cttccacaaa tttaataatc
    17341 gccccaagcc caatcaacat aaacagggtt aaaaaaatcg cggataaaat cgttttgccg
    17401 atgtagcgtt ctaaaatatt cattgagaaa aattgcatta tgccaccgcc ttattggtta
    17461 atttttttgc accaaattga tagcgtaact tagacataaa tttactatcc cacgcattta
    17521 aaataacacc taaaactaaa aaggcaactg ataccaacgg cattaacact tccgcattca
    17581 acttgcctga tgcccctgac gattttagcg agctttgtag taagaaatag attaaataaa
    17641 gcaataacgc cggcagtagt ttagcaaaac gcccttgacg agggtttaca ctactcattg
    17701 gcaccgctaa taatgccatt aacggtacag ctaaaatcaa agcaagccgc caatgaagct
    17761 cggcttttgc ttccggcgtt ttggtttcca ttaatttctt gaaatcggct cgttgcacta
    17821 atttctcatc agattcaacg tcttgatagc ctaaataagc agaataattc tcaaaatgtg
    17881 aaatacggaa atcggcactt tgtggtgtac cttcaaaacg cttgctctct gccaaggtta
    17941 agacttgatc cccatttggc aaaccttgca acgtgccgga ttcagcaaca accactgaag
    18001 gtttgttttt cttctgctgt tccggctgga aaacgtagat atcatttaga ctgttatttt
    18061 caattttatc aataaataac acatagccac cggcagacat aaattgtccg gccgaaagag
    18121 ccgcaaaacg tgggttggct ttcgcttccg ctaacatttc actctgttta ttaattgccc
    18181 aaggcgtaag ccaaaacacg ttataaaccg ctaatgcagt ggtaaaaatc gagagaaaca
    18241 tcgccacttt aaccaatagc ccttgcccga taccacaagc ccgcattacg gtaatttcac
    18301 tttcggcata aaaccgcccg agggttaata acaaggcgat aaacagtgag agtggcagca
    18361 ttaattgtgc cattgtcggc atacctaagc ctaataagct caccactaaa tctgccggta
    18421 ctttaccact cacagcagaa cttagaacac gcactaattg ctgacaaaaa aagatcagta
    18481 ataagataaa caatatcgcc aattggcttt taaatatctc tttggttaaa tatcggctta
    18541 agatcacgtt ttttctctat aacatttaca aatctgaata ccgctaaaca caagcggtct
    18601 atttttgtta attttttgca aagagcgcaa aataacttat tcgaatcgct aataatacct
    18661 ttttcataca aaaaaatcac ctatagaaaa tatcacgcac ggcaaaaccg tgcgtaagta
    18721 ttattgtttt ttcgattttt tcgatgatgt tgctttatga gcttcaagaa ttaatttctt
    18781 accttggcta tcttcaattt caatcttagg tggtgcttcc gtattttgat gaaggatagc
    18841 tataatattt tcaccttgtg caatatcttc aaagtttcct ttcacacgta acttagccgc
    18901 atcaacacta gtgtcaaata ctaagaatgt acctaatgcg gtaactctaa tatttttaag
    18961 ccctatattg tccttcgtta ccgttttata ttgaggtgct tttttccctt ttgcagccat
    19021 aatcttctct tgattagtcg caatattaag aatattttga actttagtcg cttctgctgt
    19081 agattgtgcc tgttctgtta aatttggttc tccatcaaaa ataacatccc caacaaattg
    19141 cacctcttta ggctcatttt gcaataatgg tgcaacgtct ttagctgaaa caaccaaaaa
    19201 taaccgttca cctatttttt caaaacggac atcaatattg gttaattgtg attcaatttg
    19261 ttcattaatt gtcttgatcg cagtttctgt ctcattttga agcgatattt tacgtgtaat
    19321 atcaacatca taagcaataa aaccaacttt taaacgttgt gataactgct gaagtaaatt
    19381 gcccacattt ccatcataag aaagacgtaa ctttgcctgc tcatagcgac taatttgagc
    19441 atcatcatta gcgaaaatag tgttttgagt ccctaagccg attagaccaa caacaaacgt
    19501 tttaccccaa aagcgcatca tttttatctc caattattct cttaaatcta aaaaactctt
    19561 ttagtataaa gtaagcaaac taaaagaaaa accagtaaaa ttatctatac gaccgcaaaa
    19621 aacatttaat atttgcaaat aaaaagtaaa ttattttaaa taatgattta catcagggta
    19681 tatctatatt ttacttatca ttgatgatta tgttggcatt ataaaatctg tagcaataaa
    19741 aattaaggag gaaaaatgga atttagtgtc aaaaatggca gtgttgagaa acaaagaacc
    19801 gcctgtttgg ttgttggtgt ctatgaaccc cgccgattat gttctgccgc agaacagctt
    19861 gataaattaa gtaacggcta tatcagcaca ttactgagaa gaggagattt agaaggcaaa
    19921 gcagggcaaa ccttactctt gcataatgtg ccgaatgtac tcgctgatcg agtattatta
    19981 gtcggctgtg gaaaagagcg tgaattaact gaacgccaat acaaacaaat tattcaaaaa
    20041 agtgtacaaa ctattaatga aaccggttca atggaagctg tcagcttttt aaccgaactg
    20101 cacgttaaag gtagaacacc ttattggaat gtgcgatttg cggtagaggc aatccaagaa
    20161 caactatatg cctataacga tttcaaaagc atcaaacctg aagttcgccg tgaattacgc
    20221 cgtgtgattt ttaatgtcgc acaccgtaaa gatcttcctg atgccgaacg agctttagaa
    20281 cacggaaaag ccgtagcact tggaatcagc cacgctaaaa atgtagcaaa ttgtccgcct
    20341 aatgtgtgta atcctcacta tttagccgaa ttggctaagg gtttagccta cgactatcct
    20401 gccatttcaa ctaccattgt tgatgaaaaa cagatggcag aattgggaat gaatgcttac
    20461 ttagcggttt ccagagcctc tgaaaatgag gcctttcttt cagtgattga atataaaggc
    20521 aacccgaatg ctgatgcgaa gccgattatc ttagtcggca aaggaatgac ctttgactct
    20581 ggcggtttat ccattaaacc tgcggaggcg atggacgaaa tgaaatacga tatgtgcgga
    20641 gctgctactg tttacggtac aatcaaagca attgccgaga tgaaattacc actcaatgtt
    20701 atcggcttac tcgccggctg tgaaaatgcg gttgatggca aagcctatcg accgggggat
    20761 attctcacta caatgtctgg tttaaccgtt gaggtactca acaccgatgc agaaggacgt
    20821 ttagtgctat gtgatgctct cagctatgta gaacgttttg aaccggaatt agtgattgat
    20881 attgctaccc taactggtgc ttgcatgatt gccttaggta atcataacag cgggctgatg
    20941 tcacctcata acggtttagc aaacgaactc ttagctgccg cagagcaagc cgatgacaaa
    21001 gcgtggcgtt tacctttagg ggaagaatat caagaacagt taaaatctaa ctttgctgat
    21061 cttgctaata tcggcggacg tttaggcgga gcctgtactg ccggtcaatt cctctctaac
    21121 tttacaaaga aatatacttg ggcgcatcta gatattgccg gcacggcttg gaaatctggg
    21181 gcagccaaag gggcgaccgg cagaccggta tccttactaa cacaattttt gatcaataaa
    21241 gcccaggtct aactctcaca agcggtctga ttttatcgag tttttgcaaa aagaaaatca
    21301 gaccgatcct cttcatacgc ccctatattc tttttagtta tatcaaataa attccagcta
    21361 ctagaaaagc gattaaaaat aagtatgata aatcccaatc tcaatactta ttaaggaata
    21421 tcactatgac tatctatgca gacaactcat actcaatcgg caataccccg ctggtgcgtt
    21481 taaagaattt tggcaacggc aatattgtgg taaaagtgga agggcgtaac ccaagtttta
    21541 gcgtgaaatg ccgtattggt gcgaatatgg tgtggcaagc agagaaagac ggtattctaa
    21601 ccaaagacaa agaaattgta gatgcaacca gcggtaacac tggaattgcc ttggcttatg
    21661 tagcagccgc tcgtggttat aaattaacgc tcacaatgcc ggaaacaatg agtaccgagc
    21721 gtaaacgctt attgcgtggc ttaggagtaa atttagtctt aaccgaaggg gcaaaaggaa
    21781 tgaaaggagc gattgcgaaa gctgaagaaa tcgcggcttc cgacccagat cgctatgtta
    21841 ccttaaaaca gtttgaaaac ccggcaaacc cggcaattca ccaacaaact accggtcctg
    21901 aaatttggga tgcaactgaa ggtaaaattg atgtattagt ggcaggcgta ggaacaggtg
    21961 gcactattac cggtgtttcc cgtgctatta agcaagataa aggcaagcaa attatttcag
    22021 tggcggtaga accgagtgaa tcgccggtca ttacgcaaac tttaaacggt gaagaagtca
    22081 aaccgggccc gcacaaaatt caaggcattg gtgcaggctt tattccgaaa aacttagatt
    22141 tatcactgct tgaccgtgtt gaacaagttt caagcgaaga agcgattaca accgcacgcc
    22201 gtttaatggc agaagaaggc attttaggcg gtatttcatc aggtgctgcc gttgctgccg
    22261 cagatcgcat cgctaaacta cctgaatttg ccgataaatt aattgtagtg attctgcctt
    22321 cagcctcaga gcgttattta agtacgctac tgtttgaagg gattgaagcg taatattaaa
    22381 ttaatataaa aatgccggct gatgtcggca tttttattgc aaacaagcgg ttatttttta
    22441 taaaaaattt gcaatttgtc tcctattgcg ttattcttcg ccacatcttt acggttggcc
    22501 gtatctaatc aatatcaaca aatccaatct taattaatca caattttaca ttcaaattat
    22561 gcatcttact caacttaaaa acacccctgt ttcaaagctt gtggaaatgg gtgaaaatca
    22621 aatgggctta gaaaatctgg ctcgtttacg taaacaagac atcgtatttg ctattcttaa
    22681 acaacacgca aaaagcggtg aagacatttt cgggcaaggc gtgcttgaaa ttctaccgga
    22741 cggcttcggt ttcctccgct catcagatag ctcatattta gccggccctg atgatattta
    22801 tgtttctcca agccaaatcc gccgttttaa cttacaaact ggcgataaaa tagaaggtaa
    22861 aattcgccca ccaaaagagg gagaacgcta ttttgcccta ctgaaagttg attcagttaa
    22921 tgatgacaaa ccggaagcct ctcgcagtaa aattctcttt gaaaacttaa cccctctgca
    22981 cgccaattct cgcttaaaaa tggagcgtgg caatggatca aaagaagatt taaccgctcg
    23041 tattttagat ttagccgccc caatcggtaa aggtcagcga gcgttaattg ttgccccgcc
    23101 aaaagctggt aaaaccgtgt tactgcaaaa tattgcacaa agcatcaccc ataatcatcc
    23161 tgaatgtgag cttatcgtat tattaattga tgagcgtccg gaagaagtaa cagagatgca
    23221 acgcaccgta cgtggtgaag taattgcctc aacctttgat gaaccggcaa cccgccacgt
    23281 tcaagttgcg gagatggtaa tcgagaaagc aaaacgctcg gtagagcata aaaaagatgt
    23341 ggtgatttta ttagactcca tcactcgttt agcccgtgcc tacaacaccg ttaccccggt
    23401 ttccggcaaa atcctttccg gtggtgtgga cgctaacgcc ttacatcgtc caaaacgttt
    23461 cttcggtgca gcccgtaatg tggaagaagg tggcagctta accattatcg caaccgcact
    23521 tgtggatacc ggatcaaaaa tggacgaagt gattttcgag gagttcaaag gtacgggtaa
    23581 tatggaatta cacctctcac gccgtattgc tgaacgccgt atcttcccgg ctattgactt
    23641 caaccgctca ggtactcgta aagatgacct cttaatggca gcagacgaac tccaaaaaac
    23701 ttggatgtta cgcaaagtcc tcaacccaat ggacgaagta gatgctatcg aatggctaat
    23761 cgacaaatta atgatggcaa aaactaacga agagtttttc gagattatga aacgttcgta
    23821 gaacataaaa aaatcccgct taacgcggga tttttatcaa tagcagctgt caataaagta
    23881 actacttaaa atcccccaac tcaaacaacc ccattgatct cgccacattt acataagtat
    23941 aaagtgcctc gattttggct tttcccggtg ttgccggctt gccaccacca acggaatcca
    24001 tctcaatcgg ataaccaatg tgctgtgcat aacctttgag aaccgctgtt gcacgaggga
    24061 tatgttgtaa cgtaccgata cctaatactt tcttgccatt ataagtttgc atcagcttga
    24121 taatcccaac cgcatttccc ggggtatctc tggcaacagg atcaagtagc aaacggtctt
    24181 tttcaatacc attttcgcta agccattttg ccatcacatc agcctcagca aattccgttt
    24241 tcacagcacc gccactgacg ataatttttg cttgcggaaa tttttgtgcc atctctttgg
    24301 ttttctgtaa tcgatctaac aacagcggcg aaggtgtgcc gtctttgttt ggcgtagaac
    24361 caaaaacggc aatcgtatca gggatcattg ctgtctgtgg ttcattaccg tagcttgccg
    24421 ccagcgtttg ttcggtaaag gcaaaaaagt ctgtcattcg ttgtgcgact tcgggagcaa
    24481 gctgtttcag tgcagaaaga cgagccgttt cttgttcagt atcttctagc acccttgaag
    24541 ctaatactgt catcgcaaga gcgtgaatat cacgggggtt ttggcgcaaa atttggtcga
    24601 gctgagaaag tgcttcccga tactctttat tgacgaaata cgcctctgct tgtaaacggt
    24661 aagtgttaag gtaggtcggc ttctctttaa ctttctgatc aagcgaagca tacatcgctt
    24721 gccaatacca acggcggaaa ttcagcacat cctcttttag ataagggtca tcaacatagt
    24781 ctggcgcata ttgaataact aaatgctcaa tcggcgctcc tcgaccatct tctttacctt
    24841 ttggtgcagg cagtactttt gctaatacaa agttaaacgc cttatcatat tgactgatat
    24901 tgtcttggct caaataatca gctggtatcg cccagctatt tacacttatc aaagaaaggc
    24961 ttaaagcaag ggcagaaagg gttgttttga tttttttcat agaggctcct atttaccatt
    25021 gatagtgata atttgcgtgt aatccgtatg aacgcttcaa tgcatccccg tgtaacattg
    25081 atccttcgat tgcaaaataa tggcgatttc caactgcttg tatcccaata ctgattacat
    25141 tccgatgacg atcagataaa ctatctaagg tatcaatatg acgatttcga aaacgatagc
    25201 taatatcagc ataaggttta agtttgattg cattgagttc aatcactttt cctaaacgta
    25261 gccctgtccc agcccaaaac attcgtgctg atccagcttt tgcccagcct aattgcagct
    25321 ttggcgtaat ttgccattga tgactcatca cgatatttct tccaatttca ctactcagac
    25381 tgtatgcatt attatctcgt tttgttacac cagattgatt agtaaaatga ctgtaattaa
    25441 aaaaatttgt ccaaaacaga ccacttgtag gacgataacc agcataaata ccagcagatg
    25501 tccctttcac tttatgcgtc ctattctcaa tttgcccctg actgatacca aatactccac
    25561 caatagacca attatgggaa acctgatgat ctagcccgat atgtaataag tgatagtctt
    25621 cggttgaacg attttttaaa cgatatttgg atagactatc cgaaagccat aaattccaat
    25681 cttgtgtatg ttctatttct gctaacctac ggtacagatc ataagttgct tgttccatca
    25741 aattaagctg tacaacataa tgctcggaaa aaggattgtt ctgctttatt attaaacgac
    25801 taagggcttc cgtactctgt tttaactgct cttgcgtttt actaagttct ttattcaatt
    25861 cctcttcagc ttgctgatga gcctgttttg ctgtttctaa cgcctgtctg gctacttcca
    25921 gctcatttgc agaattttct gcatttttta ctgcttgatc tcgctcagct accaattttt
    25981 ctaaataatt ctgctgtgct ttttccaacg cttctctttg ttctttttca gcttgttctt
    26041 tggcaagcaa attctcaaaa tcgcccaagg taaataaccc cattgcacga gcaacattaa
    26101 caaatgtata aagagcttca ccttttttat ttttttcatt aggaggagaa ccgcctccgg
    26161 cagaatcaat ttccaattca tatccaatgg aatccgcata gcttttcaat acagacattg
    26221 ctctcggtaa atgtaagatc gtaccgatcc ccaatacttt tctacctcca tattccttca
    26281 taaaatcaat gataccgaca gcatttcccg gagtatctcg tgccacatca tcaagcaaca
    26341 gtctgctttc atcaacgcca ttctctatca accatttttt cattacatcc gcttctgcat
    26401 agatataacg aacaggtcca ccacttaaaa taatttttgc attcggaaat ttctctgcca
    26461 tttctttggt tttctcaaga cgttgcaata atcccttgct tggagtaccg tctgggttag
    26521 gcgattgccc aaataccgct atggtgtcgg ggatcattgt tgtttgaggc tcactaccat
    26581 agctggcagc aagcatagtg tctgtaaaat gtagatattg ctgcaccttt tgcgcaatac
    26641 ttggagagat ttgataaagc gctaataaac gctcggcttc agtattagga ttttccaata
    26701 cccgacttgc aaccacactc attgccattg catgaacatc attagggtta cgccgcagaa
    26761 tttgatccag ttgagataat gcttcttggt attgcttatt gataaagtac gcttccgcct
    26821 gtaatcggta agtatttaaa taatcgggct tttgtttcgt ttttaaatcc aatgacgtat
    26881 agagatcttg ccactgccaa cggcgaaaac gaataacatc ctctttaaga tagatatcat
    26941 taagataatc tgatgcatat ttcaccacca aattctcaat cgcttcacca cgcccatctg
    27001 cttttgcaat aggcgcaggt agtggatttg cgaggaaaaa attaaaacgg gcatcaaatt
    27061 gccgaatatt atcgttactt aaataaccgt cagcataagc cgataaaggt aaggctgaac
    27121 ctaatatcaa agcaagataa gaataacgca agcgtttcat tatgatttct ccttatattg
    27181 ggtgaagaag tatttataac tttttacatc taaccaacaa agttgattaa ggataaaatt
    27241 tgtttgtgct attttattaa taatattcaa aatcattttt aatttttgtg gttgctcaat
    27301 gacatt
//
LOCUS       DANLXQ010000016        26146 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.016, whole genome shotgun sequence.
ACCESSION   DANLXQ010000016 DANLXQ010000000
VERSION     DANLXQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26146
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.016"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            56..1048
                     /locus_tag="PXD94_000520"
     CDS             56..1048
                     /locus_tag="PXD94_000520"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL6285415.1"
                     /translation="MKKSFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSEVGNGM
                     QDNLSGIEKAVKVNVKCIPSATYEVVHSLAKWKRHTLARFGFKEGEGLFVHMKALRPD
                     EDSLDRTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSV
                     LPKQITFVHSEDLVQRYPNMTSKERENAICKECGAVFLIGIGGKLSDGKPHDGRAPDY
                     DDWTTESEQGYKGLNGDILVWNEELGTAFELSSMGIRVDETALRLQVALTGDEDRLEM
                     DWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(1172..1591)
                     /gene="atpC"
                     /locus_tag="PXD94_000521"
     CDS             complement(1172..1591)
                     /gene="atpC"
                     /locus_tag="PXD94_000521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL6285416.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADMTAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(1619..2992)
                     /gene="atpD"
                     /locus_tag="PXD94_000522"
     CDS             complement(1619..2992)
                     /gene="atpD"
                     /locus_tag="PXD94_000522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL6285417.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(3013..3879)
                     /gene="atpG"
                     /locus_tag="PXD94_000523"
     CDS             complement(3013..3879)
                     /gene="atpG"
                     /locus_tag="PXD94_000523"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL6285418.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKAISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKSRLAVVGNKGISFFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAY
                     REGEIDEVYVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLD
                     SLLVRYLESQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITN
                     ELNEIVAGAAAI"
     gene            complement(3905..5446)
                     /gene="atpA"
                     /locus_tag="PXD94_000524"
     CDS             complement(3905..5446)
                     /gene="atpA"
                     /locus_tag="PXD94_000524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL6285419.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(5459..5992)
                     /gene="atpH"
                     /locus_tag="PXD94_000525"
     CDS             complement(5459..5992)
                     /gene="atpH"
                     /locus_tag="PXD94_000525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL6285420.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(6006..6476)
                     /gene="atpF"
                     /locus_tag="PXD94_000526"
     CDS             complement(6006..6476)
                     /gene="atpF"
                     /locus_tag="PXD94_000526"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL6285421.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRVIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDAAANNDIIDKLVAEL"
     gene            complement(6537..6791)
                     /gene="atpE"
                     /locus_tag="PXD94_000527"
     CDS             complement(6537..6791)
                     /gene="atpE"
                     /locus_tag="PXD94_000527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL6285422.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(6909..7697)
                     /gene="atpB"
                     /locus_tag="PXD94_000528"
     CDS             complement(6909..7697)
                     /gene="atpB"
                     /locus_tag="PXD94_000528"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL6285423.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(7720..8103)
                     /locus_tag="PXD94_000529"
     CDS             complement(7720..8103)
                     /locus_tag="PXD94_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL6285424.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
     gene            8571..9704
                     /gene="carA"
                     /locus_tag="PXD94_000530"
     CDS             8571..9704
                     /gene="carA"
                     /locus_tag="PXD94_000530"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL6285425.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIVTLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSQKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            9807..13013
                     /gene="carB"
                     /locus_tag="PXD94_000531"
     CDS             9807..13013
                     /gene="carB"
                     /locus_tag="PXD94_000531"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL6285426.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPKSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(13088..13879)
                     /locus_tag="PXD94_000532"
                     /pseudo
     CDS             complement(13088..13879)
                     /locus_tag="PXD94_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            14082..15080
                     /gene="yiaK"
                     /locus_tag="PXD94_000533"
     CDS             14082..15080
                     /gene="yiaK"
                     /locus_tag="PXD94_000533"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL6285427.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNLRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            15205..15570
                     /locus_tag="PXD94_000534"
     CDS             15205..15570
                     /locus_tag="PXD94_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL6285428.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNF"
     gene            15634..16113
                     /locus_tag="PXD94_000535"
     CDS             15634..16113
                     /locus_tag="PXD94_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL6285429.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            16114..17391
                     /locus_tag="PXD94_000536"
     CDS             16114..17391
                     /locus_tag="PXD94_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL6285430.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVVAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            17494..18480
                     /locus_tag="PXD94_000537"
     CDS             17494..18480
                     /locus_tag="PXD94_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL6285431.1"
                     /translation="MKLFNLKTLFALVAGVAVMSGSAMAETSLRFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEVRKAFVEKHGDELLKKVDALAQ"
     gene            18554..19537
                     /locus_tag="PXD94_000538"
     CDS             18554..19537
                     /locus_tag="PXD94_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL6285432.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSKLYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            19668..21152
                     /locus_tag="PXD94_000539"
     CDS             19668..21152
                     /locus_tag="PXD94_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6285433.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVVVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMHLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            21347..22393
                     /locus_tag="PXD94_000540"
     CDS             21347..22393
                     /locus_tag="PXD94_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6285434.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            22406..23515
                     /locus_tag="PXD94_000541"
     CDS             22406..23515
                     /locus_tag="PXD94_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL6285435.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            23620..24510
                     /locus_tag="PXD94_000542"
     CDS             23620..24510
                     /locus_tag="PXD94_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL6285436.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIAILPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            24582..26051
                     /locus_tag="PXD94_000543"
     CDS             24582..26051
                     /locus_tag="PXD94_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL6285437.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPIVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHLLQAIYEGVLFSLMSHLNRMFIR
                     FPKADVLRVTGGPVKSKVWMQMLADLTGMRLEIPQMEETGCLGAALMAMQGVGLDISQ
                     VQALNNEMQVFEPNPENYAAYQTKYQRYQKLTEALNTMI"
BASE COUNT         7508 a         5746 c         5714 g         7178 t
ORIGIN      
        1 tgcaatcaag cacgagaaat aaaaaatcaa cattctttaa ttaggaaagc aaacaatgaa
       61 aaaatctttt attttacagc aacaagaaat cagttttgtt aagaacacat ttactcaata
      121 tttaattgat aaattaggca ttattgaagt tcaggggcct attttgagcg aagtcggcaa
      181 cggtatgcaa gacaaccttt ccggtatcga aaaagcagta aaagtaaacg taaaatgtat
      241 tccaagcgct acttatgaag tggttcattc tcttgcaaaa tggaaacgcc acaccttagc
      301 tcgttttgga tttaaagaag gagaagggtt atttgttcat atgaaagctc ttcgccctga
      361 tgaagattct ttagatcgca cacactcggt atatgttgac caatgggact gggaaaaagt
      421 cattcctgaa ggtcgccgca acttcgattt cttaaaagaa acggtaaacg aaatctataa
      481 agcaattcgt ttaaccgaat tagcggtgga ggctcgtttc gatattccat ctgtattgcc
      541 taaacaaatt actttcgttc acagcgaaga cttagtgcaa cgctatccga atatgaccag
      601 caaagagcgt gaaaatgcta tctgtaaaga gtgcggagcg gtgttcttaa tcggtatcgg
      661 cggcaaatta tcagacggta aacctcacga tggacgagct ccggactatg atgactggac
      721 aaccgagtcc gaacaaggct acaaaggctt aaacggcgat attttagttt ggaacgaaga
      781 actcggcaca gcctttgaac tttcctcaat gggtattcgt gtagatgaaa ccgccttacg
      841 cttacaagtt gctttaactg gcgacgaaga ccgcctcgaa atggattggc acaaagattt
      901 attagccggc agactcccgc tttctatcgg cggcggtatc ggtcaatctc gcttagtgat
      961 gttcttacta cgcaaaaaac acatcggtga ggtgcaatcc agtgtctggc caaaagcggt
     1021 atttgaagag tttgagaata ttctttaata aaacaaaaaa gcgaacctta gggttcgctt
     1081 ttgctttcta caagcggtct tattttgcaa aaaatttact gtttttaacc gcttgtaaaa
     1141 caaaacccca ctttcgtggg gttttttgat attaacgttt attcgctaat ttggttttag
     1201 tgagttcgta aacacgcagt ttcgcaattt ctctcgctaa cttcgcggtc atatccgcat
     1261 cgctggtttt tgaaagtgtt tcttctactt tgcgttttgc ggcaagaata cgctgttcgt
     1321 ctagttcttc accacgaatt gcggtatcag ccaacactgt tactacggtc ggttgtactt
     1381 caaggaaacc acctgaaacg tagataaact cttctttgcc gtcagctaaa gtgaatttaa
     1441 ccattcccgg tttaattgcg gttaataacg gtgcatgccc tgcatacacc cctaattcac
     1501 catctgtccc tgaaacacgc acgctggtaa ctgtgccttc aaagatttta cgttctgcac
     1561 tcactaccgt gagttcaaat tgagatgcca tgttgttctc ctttagttta gaacaaaatt
     1621 acatctgttt tgctctttca accacttcgt caattgaacc cgccatatag aacgcttgtt
     1681 ccggaatatg gtcgtattcg ccttctaaaa tgcctttaaa gccacgaatt gtttctttta
     1741 acggtacgaa tttgcccggt acgccgttga atacttccgc aacgtggaat ggttgtgaaa
     1801 ggaaacgttc gattttacgc gcacgagcaa caaggcgttt gtcgtcttcc gataattcat
     1861 ccatacccaa aattgcaatg atgtctttta actctttata acgttgtaat gtaccttgta
     1921 caccacgagc aacgttatag tgttcctcac caaccacgag tggatctaac tgacgagagg
     1981 ttgagtcaag agggtcaacc gccgggtaaa taccgagtga agcgatatta cggcttaata
     2041 ccaccgttga gtctaagtgg gcaaaggtag ttgccggaga cgggtcagtt aagtcatccg
     2101 ccggaacata taccgcttgc acagaggtaa tagagccggt tttggttgag gtaatacgct
     2161 cctgtaatac gcccatctct tctgcaagag tcggctgata acctaccgca gacggcatac
     2221 gacctaataa cgcagatact tcggtaccag ctaaagtata acggtagatg ttatccacga
     2281 agaataatac gtcacgacct tcatcacgga atttttctgc catggttaag ccggttaatg
     2341 ccacacgcag acggttgccc ggtggttcgt tcatttgacc gtaaaccagc gatactttgt
     2401 ctaatacgtt agagtctttc atttcgtggt agaagtcatt accctcacga gtacgctcac
     2461 ctacaccggc gaataccgag taaccgctat gttcgatagc gatgttacgg attaactcca
     2521 tcatatttac ggttttacct acacccgcac caccgaataa gccaacttta ccacccttag
     2581 caaatggagc aactaagtcg ataactttga taccggtctc taaaagctcg gtgctgttcg
     2641 cttgttcttc gtagcttggt gctgcacggt ggattgtcca acgttcttct tcaccgattg
     2701 ggcctgcttc atcaattggc tcacctaata cgttcataat acgaccgagt gtttttgtac
     2761 caaccggtac ttcaatcgct ttattcgtat tttcaacttt taagccacgt tttaaaccat
     2821 cagatgaacc aagtgcgata caacggacca caccgccacc taattgttgt tgaacttcaa
     2881 gggttaatcc tgattcaact tttaatgcat cataaacttt tggtactgca tcttgcggaa
     2941 actcaacatc gattaccgca ccgatgatct gtacaatttt tcccgttgcc attaccgttc
     3001 ctctattcat tgttaaattg ctgcggcacc cgcgacaatt tcatttaatt cattcgtaat
     3061 acttgcttga cgagctttgt tatacactaa ctgtaactca ttaatcagat tacctgcgtt
     3121 atcggttgct gctttcattg ccaccattcg agcagcctgt tcagacgcaa gattatctac
     3181 cactgcctga taaacttgag attctaaata acgaaccagt aagctgtcta ataacgcttg
     3241 tggatttggt tcatagagat aatcccaaga accagtttgc tctaatgagt cattttccag
     3301 tgcaggcaaa ggaagcaact gttgtactgt cggtttttgt gacatcgtgt ttataaaacg
     3361 gttatatacc acatacactt catcaatttc gccttcacgg taggcattta ccataccatt
     3421 aacaataccg actaaatctt ccattgccgg tgtatcaccc aatccattga tatgaccttt
     3481 aatctctagc cccattgggt taaaaaagga gatcccttta tttcccacca cagcaagtcg
     3541 agacttaaca ccttggtcat ctttttcttt caattcatgt aaaacggttt taaataaatt
     3601 gatatttaag ccaccacata aaccgcgatc ggttgaaact actaaatagc caactttttt
     3661 taccggacgt ggggtcaaaa atgggtgttt atactcaatg ttacctttgg caatatggct
     3721 aattgccttg cgaatacttt cagcataagg gcgagaagcc gccatacgct cttgggtttt
     3781 acgcattttt gatgcggcaa ccatttccat cgctttggta attttttgtg tattacgaac
     3841 acttgcaatt ttggttctta tctctttagc acctgccata ttatttctcc gctaaaaagt
     3901 tagattacca agcactgttc tttttgaaac cagataaaat ctcgttcaat ttatctttaa
     3961 tttcatcgtt gaaattgccg gattttgaca gttcttgcat aaactcgccg tagttacttt
     4021 gagcataagc taataagttt gcttcaaatg agccaacacg ctcaaccgga atatcatcta
     4081 agtaaccgta ttcagctgct gctaatgata aacctaattg tgcaacagac atcggctcga
     4141 attgtttttg tttcaacaat tcagttactt tttgaccgtg tgaaagctgt ttacgtgttg
     4201 cttcatcaag atctgatgcg aactgggcaa atgccgctaa ttcacggtat tgagcaagtg
     4261 cggtacggat accaccggca agcttcttaa ccagtttagt ttgtgcagaa ccacctacac
     4321 gggaaaccga gatacccggg tttaccgcag ggcgaatacc ggcattgaat aagcctgatt
     4381 ctaagaaaat ctgaccatcg gtaattgaaa ttacgttagt cggtacgaag gctgaaacgt
     4441 cacctgcttg ggtttcgatg atcggtaatg cggttaaaga accggtttga cctttcacag
     4501 caccgttagt gaaacgttct acatagtctg catttacacg agacgcacgc tctaataagc
     4561 gagagtgtag atagaacaca tcacccggga atgcttcacg acccggtgga cggcgtaata
     4621 acaatgaaat ttgacggtaa gcaactgctt gcttagacaa atcatcgtaa acgatcaacg
     4681 catcttcacc acggtcacgg aagtattcac ccattgcaca acctgcataa ggtgcaagat
     4741 attgtaatgc agcagattca gacgcagaag ccactacaac gatagtgttc gctaatgcac
     4801 cgtgttcttc taatttacgc accacgttgg cgatagttga tgccttttga ccgattgcaa
     4861 cgtagataca tttaatgcct gaatcacgtt ggttgatgat cgcatcaatc gctaatgcgg
     4921 ttttaccggt ttgacggtca cctataatca attcacgctg accacgaccg attggtacca
     4981 ttgagtccac cgctttgtaa ccggtttgaa ccggttgatc aaccgattga cggtcgataa
     5041 cgcccggtgc gatcacttcg attggtgaga aaccgtcatt ttggatttcg cctttaccat
     5101 cgattggctg acctaaggta tttacaacac gacctaataa gccacgacca accggcactt
     5161 ctaaaatacg accggtacat tgtacttgca taccttctgc taaatcggca taaggtccca
     5221 ttacaactgc accaacagaa tctctttcta agtttaatgc aatcgcataa cgattgcccg
     5281 gtaatgcaat catttcacct tgcattacat cagataagcc gtggatacga ataaccccgt
     5341 cacttaccga aacgattgtc cctgtgcttt gagcttcact caccacatta aactgggcaa
     5401 tacgtttttt aatcaattca ctaatttcag ttgaatttag ttgcattttt tattcctctt
     5461 acaagctcaa ctcttgactc aagcggttta gctgtccacg actactacca tcaatcacta
     5521 catcatcata acgaatgatc acacccgcaa ttaatgaact atctattgat gaagtaatac
     5581 gtactttcgt tccgagtcgc ttttccatcg cattggcaat ttttgcttct tgtgcatctg
     5641 ttaatttatc tgcagaaaca acctcaacat ctttaattga ttcctgttct gctcttaatt
     5701 ctaaaaatgc attcaatacc gcaggtaata ctgctaaacg cttgttatca gccattacgc
     5761 gaatgaaatt ttgcccatat tgatctaatt gttcgccaca aatcttgata aacgtctcag
     5821 aaatctgacc gcttgcaagt gatgagttaa gaaaactagc tacttgctca tcgttagcga
     5881 cggcggcaga aaaagttaac atctgctgcc attgttccaa ttgaccttgt tctaaagcaa
     5941 aatcgaaagc cgctttagca taggggcgag ctactgtgct taattctgac atctgcccct
     6001 cctagttata gttccgcaac tagcttatca ataatatcgt tgttcgcagc ggcatccacc
     6061 gaacgaccca cgattttttc tgcaccagca atagccaatg cagccacttt ttgacgaagc
     6121 tcttcttgaa cgcgtttacg ttcgctttca acttccgcgt aaccctgttc aataatacgt
     6181 tgacgttcca cttcagcttc tgcttgtaca gattctaaaa tttcattacg acgttttgtt
     6241 gctaaatcaa tgatattttg tgcttcttct ttcgctctaa ggatttcttg atccgcagcc
     6301 gctttagaat cagcttgttc ttgtttcgct ttttcggcag aagccaatgc gctagcaata
     6361 tttgcctgac gctcttctat cactcgaatt agcggtggcc atacaaactt catacagaac
     6421 gcaacaaaaa gtgcgaacgc aatcagttga ccaattagtg ttgcatttaa attcacaacg
     6481 ccctcctaaa agccggtaaa gttattcaac tgaataacgg ttaatcaaat cccgacttat
     6541 ttcaataaat caatgaacgg gtttgcgaag atgaatagta atgaaatacc tacagcgatc
     6601 attgcgatag catctaaaag accagcaacg ataaacattt tagtttgtaa gcttgatgct
     6661 aattccggct gacgtgcaga tgactctaag aatttgccac ctaagatagc aaagccgata
     6721 gcagtaccaa gagcagcgaa agctaataaa attgaagcac caataattgt tgctgtgatt
     6781 actgattcca taatgttctc cattagattg aggaattagc ccgaaggctg ggttgttgat
     6841 aaaaatacat atcaagcggt caatattttg caaaaatttg cgaaaaacct accgcttgta
     6901 aggctagttt aatgatccgc tttgttataa gcgatactca aataaacaat cgttagcatc
     6961 ataaaaataa aggcttgaag cgtaataact aaaatatgga aaatcgccca aaccaagtgt
     7021 aatggaatcc ccaacgcctg tagagcgaag ttgtctgcca tatacattac tgcaataagg
     7081 ataaagatta actcacctgc atacatatta ccgaataaac ggaacgccaa tgaaatcggt
     7141 tttgccaaaa gcgttaccgt ttcaagaata aagtttaccg gaatgaatgc ccagtggtta
     7201 aacgggtgta acgtgtattc tttcactaaa ccgccaaaac ctttcgattt caccgtgtaa
     7261 aataaaatta acgcaaatac acaaatagac atacccagtg ttgcactaat atctgccgtt
     7321 ggtactgcac gcaaatagtg aataccaaac agattggcta actgtggtaa gaaatcgacc
     7381 gggataaggt cgatcgcatt cataataaac acccaacaga aaatcgttaa tgctaatggt
     7441 gcaattacat tgcgaggtcc gtggaagtta tccttcacta aaccgtttac ccactcaata
     7501 acaatctcaa caaaacattg caattttccc ggaacgcccg ttgttgcatt ttttgctaca
     7561 cgcgagaaca cccataaaaa aatcgcacct gccactaaag agaagaacag tgtatctaaa
     7621 tgcactgccc aaaagccttc acctgtcgat aaaaaactca agtgatggct aatatattct
     7681 gctgtcgttc cagccataac atatcctttt aaattaaaat tagtggtgat gtttgcttaa
     7741 tacaaacggg atcatattat ttaaaaataa tgcgataaaa taacccacga aaaacaacac
     7801 aacatctaga gcagggataa gtttaaaaca gccgatcacc aaaataatag taaccaacca
     7861 tttcattcct tcaccacgat agaaagcggt catttttgac tgattttttg cagatttctt
     7921 aaagaaaacc caatatacaa acacaagatg aggcaaaaaa ctcgctaaag caccgcctag
     7981 aaaggaaaaa ttaaaattca ttttccataa agacactcca agtgcaaaaa cgcacaagag
     8041 aaaaaattca acctttaatg cttgtttata ttgtgatttt gctctattga ttacagctga
     8101 cacattttat cctttttgaa taagtaaacc acacaatcag gctaataatt agccactgtt
     8161 gaatcaaaga aaatccgtgc taaattatac tttacaaatg aattgttgca accgaaaagc
     8221 gacacaaaaa cttgttattc ttcacatttt tgaaaaaaaa ttttatttac gttgcatttt
     8281 tttgagtaaa accatcaaaa tcagttaaca aaaaaacaac aaatagaggt gtgtgtattt
     8341 ttctctgtca cattatttca atatttgata aattcccctt gcttaaaaat gaataaaaat
     8401 tcataataat tgcatatatt ttcacttccg agtagaaaat gaaataaaaa aagaagaaag
     8461 aaataggcaa aataactaat ttttacttga atgcaaacgt ttgcttgatt aaacttagtc
     8521 gcctacttct aaggctacac agataaaatt tgatgattag gagtaaaaca atgttcaaac
     8581 cagcaattct ggttctcact gacggttctg tcttttccgg caaatccatt ggtgctgacg
     8641 gtcacactat cggtgaagtg gtatttaaca ctgcgatgac cggttatcaa gaaatcctta
     8701 ccgatccttc ttatactaaa caaatagtca ccctgactta cccccacatt ggcaataccg
     8761 gcattaattt agaagataaa gaatccccca atgtttgtgc ctccggctta attattcgtg
     8821 atcttcctct tctccatagc aacttccgtg cgtcctcctc actttcagat tatctcattc
     8881 aacaaaacat tgtcgctatt gccgatattg atacccgccg tttaacccga attttacgcg
     8941 aaaaaggggc acaagccggt tgtattttag taggcgatgc cacagaagag aatattgcta
     9001 acgctttggc atttgccaac agtttcggtt ctatggctgg gaaagatttg gctcaaagtg
     9061 tcacctgcaa tgaattatat aaatggacag atggtgaatg gcaattaggt aaaggttatg
     9121 taccgcaaca acaaaccgag ttccacgtag ttgcctatga ttacggtgta aaacacaaca
     9181 ttttgcgaat gcttgccgaa cgaggctgtc gcttaaccgt tgtgccggca aaaacctctg
     9241 ctgaagctgt gcttgcgtta aatccggacg gtattttcct ttctaacggc ccgggggacc
     9301 cggagccgtg cgattatgcc attaccgcaa tccgaaaatt attggaaacg caaaaaccca
     9361 ttttcggcat ttgcttaggg catcaacttt taggtttagc ggcaggcggt aaaaccaaaa
     9421 aaatggtgtt tggtcatcac ggagctaatc acccggtaca agatttagac agccaaaaag
     9481 tgctcatcac cagccaaaac cacggttttg aagtagatga atccagctta ccatctaatg
     9541 tgcgagtgac tcaccgctca ctgtttgacg gcacggtgca ggggattgaa ctcactgata
     9601 gaccggcgtt ttcgttccaa ggtcacccgg aagccagccc ggggccgaat gatgttgcct
     9661 atttatttga ccgatttgtc agtgagatga aaaaagtgaa gtaaggacgt acctatgtgt
     9721 gtccatcaag cggtcgaaat ttgcaaaaaa cttaccaaaa atgaccgctt gtagcctgaa
     9781 ataaacgaga taaaagagag cccaaaatgc caaaaagaac agacataaac actatcctaa
     9841 ttatcggtgc cggtccgatt gtgatcggtc aagcgtgtga atttgactac tccggagccc
     9901 aagcgtgcaa agcgttgcgt gaagaagggt ataaagtggt gttggtgaac tccaaccctg
     9961 caaccattat gaccgacccg aatatggcag atgtgactta cattgagccg attgaatgga
    10021 aaacggttga aaaaatcatt gaaaaagaac gccctgatgc cattttaccg actatgggcg
    10081 ggcagacagc gttgaactgt gccttagatc tttcgaaaaa cggtgtgctg aaaaaatata
    10141 atgtagagct gatcggagct accgaagacg cgattgataa agccgaagat cgtggtcgtt
    10201 ttaaagaagc aatgactaaa atcggcttga atacgccaaa atcattcgtt tgccacagtt
    10261 ttgaagaggc cttagcagca caagagcagg ttggctttcc aaccctgatt cgcccctctt
    10321 tcacgatggg aggctccggc ggtggtattg cttataatcg agatgaattt atcgccatct
    10381 gcgaacgtgg ttttgaagcc tcgcctactc acgaattact gattgaacaa tccgtactag
    10441 gctggaaaga gtatgaaatg gaagttgtgc gtgaccgtgc cgataactgc attatcgtct
    10501 gctcgattga aaacttcgac ccgatgggtg tacacaccgg cgactccatt accgttgctc
    10561 cggcacaaac gctaaccgat aaagagtatc aaattatgcg taacgcttcg ctggcggtat
    10621 tgcgtgagat cggcgtggac accggcggtt caaatgtgca atttgcgatt aaccctgcaa
    10681 acggcgagat gatcgtcatt gaaatgaacc ctcgtgtgag ccgttcttct gcactcgcat
    10741 ctaaagccac cggcttcccg attgccaaag ttgcggcgaa attggctgta ggctacacac
    10801 tcaacgaatt gcgtaacgac atcaccggag ggctgattcc ggcttcattc gagccgactt
    10861 tagattatgt tgtgaccaaa attccacgct tcgcctttga aaaatttgcc aatgcagacg
    10921 accgattaac cactcaaatg aaatcggtag gcgaagtaat ggcaatggga agaaccttcc
    10981 aagagagttt acaaaaagcc ttgcgaggct tggaaaccgg catttgcggc tttaacttgc
    11041 tttccgaaga gcctgaaaaa atccgccgtg aacttgccaa ccccggacca aaccgtattt
    11101 tgtatgttgc cgatgccttt ggagcaggct tttcattgga agaagttcac cactattcta
    11161 aaatcgaccc gtggttcttg gtgcaaattc aagacttagt acttgaagaa ttagcgttag
    11221 agaaaaaaca gtttagcgat ctagatgcta acgaaatccg ccgcttaaaa cgcaaaggct
    11281 tctcagacaa acgtatcgca cagcttacca aagtcagcga aaaaatggtg cgggattacc
    11341 gccatactta cgctattcta ccggtgtata aacgggtaga tacctgtgca gccgagtttg
    11401 cctcagatac ggcttacctc tattctactt acgaggaaga gtgtgaagcc aaaccgtctg
    11461 atcgcaaaaa agtgatgatt ttaggcggcg gaccaaaccg tatcgggcag gggattgaat
    11521 ttgactactg ctgcgttcac gcctcgcttg ctctgcgtga ggcaggttat gaaaccatta
    11581 tggtgaactg caaccctgaa accgtttcca ccgatttcga cacctccgac cgcctctatt
    11641 tcgagccgct aacattagaa gatgtactgg aaattgtgcg tgtagaaaac ccgtggggcg
    11701 tgattgtgca ttacggcggt caaacaccac tgaaattagc gaatgcctta tctgatgccg
    11761 gtgtaaacat tatcgggact tccgccgaca gcattgatgc cgccgaagac cgtgaacgtt
    11821 tccaacaaat tctaaacact ctcggtttaa aacagccggc aaatcgcacc gcccgtaatg
    11881 cgttggaagc ggtggcattg gcaaatgaag taggctatcc gctggtggtt cgcccatctt
    11941 atgtgctagg tggtcgggca atgcaaattg tgtataacga tgaagagcta aaccgctata
    12001 tgcgtgaagc ggtatcggtt tccaacgaca gcccgatttt actcgaccac ttcctcaata
    12061 atgcgattga agttgatgtg gattgcattt gtgacggcga aaatgtactc attggcggca
    12121 ttatgcaaca cgttgagcaa gccggtattc acagtggcga tagtgcctgc tcactacctg
    12181 cctattcgtt aagcggtcat gttttggacg aaattcgcaa acaaaccaac gcaatggcgt
    12241 ttgctctcgg tgtgaaaggc ttgatgaacg tacaatttgc agtacaaaat gaggtcgttt
    12301 atgtgttgga agttaaccct cgagccagcc gtactgtacc gtttgtcagc aaagctaccg
    12361 gtcgcccact ggcaaaaatc gctgctcgcg tgatggctgg tgaaaccctt gccgacttaa
    12421 atgcattaga ggaaattatc ccaagcaaat tcttcgttaa agaagcggta ttcccattca
    12481 tcaaattccc gggtgtggat accattctcg ggcctgaaat gcgttctacc ggcgaagtga
    12541 tgggcatagg taataccttt gctgaagcgt tcttcaaagc acaactgggt gccggtgaac
    12601 gaattccaaa agtcggcaaa gtgtttatgt cggttattga agagcataaa cctgctttaa
    12661 tttacattgc caaacgtttc caagaacaag gctacggttt atgtgccacc ttcggcactg
    12721 caaacttcct acggcaacac ggaattgcag tccaaacggt aaacaaagtt cgtgaagggc
    12781 gaccgcatat tgtcgatgcg atcaaaaacg gcgaaattgc gttggtaatc aacaccgtca
    12841 gcggcgagcc tgaagtgatt gccgacagcc actctatccg ccgtaccgcc ttgcaacaac
    12901 gtgtgcctat ttacaccacc cttgcaggtg ctgatgctat ctcctacggc gtaaaacata
    12961 tcaatgagtt tgatgtgtat tcagtgcagg aattagaaca aaaggctaac taaaataaag
    13021 ctaaaatgac aagcggtcag attttgcaaa ttttttacaa attctgaccg cttgtgtttg
    13081 tagaggatta tttagtaaat ttatatgcta aatcattcca gcgtaattca tttttgagtt
    13141 gtggtagctc ggtattttgg ttgatatgga taaactcaat accaaaatat tcagcgaaca
    13201 ttcgtagcat atcggcatca atatctaaac taaatacact atgatgagca ccgccggcta
    13261 gaatccatgc ttgagtacct acataaaaat ttggttgaag cttccaaaaa gcatgcccaa
    13321 ctggtaaatt tggcattgga tttggtgagg cgatagcgtc taaatccgca acaaccattc
    13381 taaaacggtc tcccatatct acaacggttg cattcactgc ttttcccgcc ttactactga
    13441 aaattaagcg aacaggatct gccttaccgc caatacctag cggtcgaatg gctaatgttg
    13501 atttctcttt ggcaatagac ggacaaactt caagcatatg agcccccaat accacttggt
    13561 tttgttcatc taaatcatag gtgtagtctt ccataaagga agaaccgttt ggtaagccat
    13621 aacccatcac tttcacttca cgcacaagag ctgcggtttt ccaacctcct tcaccaccaa
    13681 atccgtagcc ttgttccatt aaacgctgaa cagccaagcc cggtaattgt ttaatcccat
    13741 ttagattttg gaaattatca gtaaaagctt taaatccacc attatctaag aatgttttta
    13801 agccgatttc aatacgagca gaatatgcga gagattctct tgctgaacca ttttcttgca
    13861 agctatcctc aacattatac aagcggtcag attttgcaaa aaaatgaccg cttgttgcca
    13921 tttctaaagt gtgatctacc tctcaaattt acaaaatttt ttataacata ttgatttaaa
    13981 atgtttttta tatttttcct ttaattgact ttaatttgtc ttaagagtaa cttataaaac
    14041 gtgattttac tatttgaaat attgaggtat tcaagatgac tattcgagtt tcctatgaag
    14101 aattaaaagc tcaatttaaa cgtgttctgt tggctcgcaa tgtgcgtgaa gagattgccg
    14161 aagagtgtgc cactgtgttt gccgacacca ctcaagcggg tgcttattca cacggggtaa
    14221 accgtttccc acgctttatt cagcagttag agaacggtga tattgtgccg gaagccgtgc
    14281 cgactaaagt gctttcactg ggggcgattg agcagtggga cgctcaccaa gccatcggta
    14341 acttaaccgc aaaaaaaatg atggacagag caatggagct tgcatctgaa aacggcatcg
    14401 gcgtagtggc gttacgtaat gcaaaccact ggatgcgtgg cggttcttac ggatggcagg
    14461 cggcagagaa aggctatatc gggatctgct ggacgaacgc ccttgcggta atgccgcctt
    14521 ggggggcgaa agagtgccgt atcgggacta acccgttaat cattgcggtg ccgactaacc
    14581 caatcacgat ggtggatatg tcttgctcaa tgtactcata cggaatgttg gaagtgcatc
    14641 gccttgctgg tcgtcaaacc tttgtcgatg ctggttttga tgatgacggc aacccaaccc
    14701 gtgatccggc aacggtcgag aaaaacctcc gtctaatgcc gatgggcttc tggaaaggtt
    14761 cgggcttatc gattgtgtta gatatgatcg ccaccttgct ctctaacggt gaatccactg
    14821 cggcagtcac cgaagataaa gacgatgaat attgcgtgtc gcaagtttat atcgctattg
    14881 aagtggatcg cttaattgac ggcaaaacca aagacgaaaa actcaaccgt attatggaat
    14941 atgtgaaaac cgcagaacgt gccgaccctg atgtggcagt acgtttaccg ggccacgagt
    15001 tcacccaaat tctagccgat aacaaagcca acggcattcc ggtggatgac accgtttggg
    15061 cgaaattaca ggcgttgtaa ttgcaaattg accggtaggg tgggcgtaag cccacgcatt
    15121 agacacaatc ataagatgac gtgggcttac gcccacccta caagcggtca tttttccgca
    15181 gaattttaca aaacggaggt atgaatgttt tttggtcata tttcacaagt taaagcgaat
    15241 cgatacccgc cggcgatcca aattgcgttg aactatctga aaaataccga ttttgatgcg
    15301 atggaagcag gggtttacga actcaaaggc agacaaattt atgtgcaggt gttggatttg
    15361 aacaccaaat cgaaacacga atttcagccg gaagtgcatc gcaattacct cgatgtgcag
    15421 tatctacacc gaggcaaaga gataatggct gcggcggtgg ataccggcac caatccgatt
    15481 gcgatggaat acaaccctga gcgggatatt caatattatc aatccgttgc caacgaaaat
    15541 gaatttcgct gcgttgaagg caatttttga cggcacggaa accattcgca aagtggtggt
    15601 aaaaattgcg atgagcgaaa ttgaggagca acgatgaaag ccttagctca attagtcgga
    15661 aaactgttag aaatcttggt ggtggtgatt ttatccgcaa tgtcgatttt ggttttcgtc
    15721 aatgtggtac tacgttacgg ttttaacagc agcattagcg tgaccgaaga ggtgtctcgc
    15781 tatatgtttg tttggttggc gtttttaggg gcggtgttgg catttagcga aaatcaacac
    15841 gttaaagtca caatgctgac cgataaatta tcgccaacgg ctcgtaatat cttaagcgta
    15901 gtcacagatt tagcgatgtt gttctgttgc tacttaattg tggacggtag ctggattcaa
    15961 tttaacctca acctgaataa ctttgccccg atttcaggct taccgcaagg catcactttc
    16021 cttgccagca cagtggcagg ggtattaatc ggtttattaa ttattgcccg cattgtggca
    16081 acggttggcg tgattgcaaa aggagaaaaa tagatgactg tggtaatttt tctttccgtc
    16141 ttgcttggct cgattttatt gggcattccg gtagctttcg ccctgttggt ctgcggtgtg
    16201 gcgttaatgt tgcatttaga tctgttcgat gcccaaattt tggctcaaca aattgtgagc
    16261 ggtgcagaca gtttctcgct aatggcaatt ccgttcttca ttcttgccgg tgaaattatg
    16321 aacgagggcg gtctctccaa acggattatc gacctgccga tgaaattagt cggtcataaa
    16381 cggggcggtt taggttttgt agcaatttta gcggcgatga ttatggcaag cctttccggt
    16441 tcggcggtgg cggataccgc agcggtagtc gcaatgctac tgccgatgat gaaaaccacc
    16501 ggttatccga ttgaccgctc tgccggttta atcggtacgg cagggattat cgccccaatc
    16561 attccgccaa gcattccgtt tatcgtattt ggtgtggcta gtggcgtgtc cattaccaaa
    16621 ctgttcttag ccggtatttt cccgggcatt atgatgggga tctgcttggc tgccctttgg
    16681 tggtggcagg caaaacgttt ggatttaatg accttctcca aagcgaccaa agaagaactt
    16741 tgcatttcat ttaaaaacag tatttgggcg ttattgttgc ctgtcattat tatcggtggt
    16801 ttccgctcgg gaatgttcac cccaaccgaa gcgggtgcgg tcgcaacttt ctatgccttg
    16861 attgtttcac tgtttgttta ccgtgaaatg aagttcaaag atttatacaa ggtagtgttg
    16921 gcggcagcga aaaccacggc agtagtgatg ttcttagtgg cagcggcaaa tgtgaccggc
    16981 tggctgatta ccgtagcgga attaccgatg atgctcaccg agctgttaga gccgttaatt
    17041 gaaagcccga ccacactctt attggtgatt atgttagcgg tgtttattat cggaatggtg
    17101 atggacttaa ccccaaccgt gctgatttta acgccggtat taatgccgtt ggtggaagag
    17161 gcaggcattg acccggttta cttcggtgtg ctgtttattc ttaacacctc catcggcttg
    17221 attacaccac cggtcggcaa cgtgctaaac gtgattaccg gcgtgtcgaa actgccgttc
    17281 gaccaagcgg ctaagggcgt aatgccttac ctcgtaatga tgatttgttt actactgttg
    17341 ttcgtgttct tcccgtcact ggtgttagtg ccgttaagct ggatgcagta atggtttgaa
    17401 ccgaaacaag cggtcgaatt gacgcaaaaa tttgcaaatt tttttggcaa aacagaccgc
    17461 ttgcaactca atttttaatc acaggagaac cctatgaaat tatttaattt aaaaacctta
    17521 tttgccttag tggcaggcgt tgcggtaatg agcggctctg caatggcaga aacctcactg
    17581 cgttttggtt atgaagcccc acgttccgat acccaacaca tcgccgccaa aaaattcgat
    17641 gaattattgg cagcaaaaac caaaggtgaa attaaattaa aactgtttcc ggacagcacc
    17701 ttaggtaatg ctcaaacgat gatcagcggc gtgcgtggcg gtactatcga cattgaaatg
    17761 tccggctcgc caaactttac cggcttagag ccaaaactaa atgtgattga tattccgttt
    17821 attttcaaag atcgtgaaca cgtttacaaa gtattagacg gcgaagttgg tcaaggctta
    17881 ttaaaagatt tagaagccca aggcttaaaa ggcttagcct tctgggacgt tggcttccgt
    17941 gctttctcca actcaaaaca ccctgtgacc aagccggaag acatcaaagg cttaaaagtc
    18001 cgcaccaacc aaaacccgat gtacattcaa gccttcacct tactaggcgg caacccggtg
    18061 ccaatgccac tttctgagct ttacaccgca ctcgaaaccc gtgccgttga tgcccaagaa
    18121 cacccaatcg gcattttctg gtcggcaaaa ctgtttgaag tacaaaaaca tttaagttta
    18181 accaaccacg gctacacccc gttaatcgtg gtgatgaaca aagcgaagtt tgacggctta
    18241 gctcctgaat tacaaaccgc attagttgaa gccgcaaaag aagccggtca attccaacgt
    18301 gatctcaacg tgaaaaacga aaaagagatc atcgaaaaat tacgcaaaca aggcatcgaa
    18361 gtgatcgaac aagtggataa cgccccgttc aaagcagcga ttgaagcgga agtccgcaaa
    18421 gctttcgtgg aaaaacacgg cgatgaatta ttgaaaaaag tggatgcgtt agcacagtaa
    18481 ttgactttat tttagtctaa agctccctcc gtttgggggg agttgttgta tagactctac
    18541 tataggagag actatgaaac tgtttaattt aaaaacttta gccgctttcg ttgccggttt
    18601 tgcactgttt tcaaccgcac aagcagaaac ctcattccgc tttgcgtatg aagctcctcg
    18661 ttctgatact caacatattg cagcgaaaaa atttaatgat ttgctgcaag aaaaatccca
    18721 aaaagggctg aaattaagcc tcttccctga tagcacgctc ggcaacgcac aaacggcgat
    18781 tagcggtgtg cgtggcggta ctattgatat tgcaatgtcc agctcctcta actttaccgg
    18841 gttagaacca aaactgaatg tgattgatat tccgtttatt ttcaaagaca gagaccacgc
    18901 ctataaagcc ttagatggag agattggtca aggtttatta accgcattag aggctcacgg
    18961 tttgaaaggg ttggcgtttt gggaagttgg tttccgagcg ttttccaact ccaagcaccc
    19021 gatcaccaaa ccggaggata ttaaaggctt aaaaatccgt actaaccaaa acccgatgta
    19081 tattcaagcc ttctctattt taggtgcaaa tccggtgcca atgccgcttt ccaagcttta
    19141 cactgcactg gaaaccaaag cggttgatgc ccaagaacac ccaatcggca ttttctggtc
    19201 ggcaaaactc tatgaagtac aaaaatattt cagctttact aaccacggtt acaccccgtt
    19261 aattgtggcg attaacaaag cgaaatttga tgcactgcca gccgagcaac aaaccgcatt
    19321 agttgaagcg gcaaaagaag ccggaaaata ccaacgccaa cttaacctag aaaatgagca
    19381 gaaaattatc gactcgctca aaaaagcagg tattgaattt gtggataact tagataccac
    19441 accgttcaaa gatgccgtat cggctgaaac ccgcaaggca tttatcgaga aaaacggtga
    19501 tggcttagtt aaagccattg atgcattggc gaagtaaaaa tagagctaca aagtgctcgg
    19561 ttaaactaac ctcacacgaa tcgtgtgagg tacaagcggt caaaatccgc aaaattttta
    19621 cacatctttc ctcaaaggag gaacaaatgg cacagcccat tttaagtatg cagcacatca
    19681 tcaaacgctt tggtagccat acggcggtga atgatgtcag ccttgatatc cactcaggcg
    19741 agatcgttgc cttactcggt gaaaacggtg caggtaaatc gaccctaatc aaaatccttg
    19801 ccggtattta tgaccgtgac gagggtgata ttcttttcca taaccaaaaa atccaatctg
    19861 cccacgctat tgagaaaggc aacaaaaagc ccatcgcttt tatccaccaa gatttaggcc
    19921 taatagaatg gatgacgatt gccgaaaata tggcatttgt aatggggttc cctcgcaaat
    19981 ttggcttgat tgactgggcg aaagtgcgag agcagtcgca aaaagccctt gattttgtag
    20041 gcattaaact ctctgccgac actcgagtgt ttgatttaag ccgtaccgaa aaagccttac
    20101 tcgccattgc ccgtgcgatt gccgtcaatg cagaaatctt ggttttagac gaacctactg
    20161 cttccctacc tgcctctgat gtagagcatc ttttcactgt gttaaaccgc ttacgttccc
    20221 aaggtgtggg aatgatttat gtgacccacc gtttagatga agtgatcgcc atttccgacc
    20281 gcattttggt gatgcgggac ggtttcccgg ttgcagaagg gcaaaccaag ctctatgacg
    20341 tcaaaggctt ggtcaaagcg attgtaggcg aagagagccg tggcagacag cgggtggatt
    20401 taccaaccga tactcctgaa attttaagcc tgcaaaatgt cgtggttggc gacaccggcc
    20461 cggtgaattt ccgtctgcac aaaggtgaga tgattgccct tgccggcttg cgtggagcag
    20521 ggcaggaaga aatcggccgc ttattatttg gggagcgaac cttagacaaa ggcgaaattc
    20581 agttagacaa gcggtcatat tctgctaaag atccgcaaga tgcgatagcc aaaggtgtgg
    20641 cattggttgc gggagaccgc acccgtgaaa gcgtgtttat gtcaatgact accagtgaga
    20701 acctgtttat caacccggta ctaagcggtc attcaccatt aaaacgctac tcccgccgtg
    20761 aagagtgggg tgagagctgg tacaaattcc aactattcga tattcgtccg aaaaatttgt
    20821 ttattgatgc cagtgcgtta tccggtggta atcagcagaa aatcgtgttg gcacgttgga
    20881 tgcacctcaa tacgcctgtt ctgattttag aagacccgac tgccggcgtg gatgtaggag
    20941 caagggcaga gatttacgac ttgctcaaca aagcccttgc agaaggggtc gcaattattg
    21001 tgatttccac cgactttgaa gagatcgctc atttgtgtaa ccgagcatta gtcttcaacc
    21061 gaggggaaat tgccggtgaa ctactgaacg aagaggtgac tttcgccaat ttgctggcgt
    21121 tggcatcgga tagtcagtcg aaaaaacaat aacttactct cgcccgcttg cgggagagag
    21181 acagctaaaa atagcgtcag gctattttta gcagagagag ggtaaacaag cggtcatttt
    21241 tcttaaaaaa tattgcgaac cctctccctc cgaaagccta acggctttct cctccctctc
    21301 ccgcaagcgg gcgaggggaa aatgcagaat tttaacggag taatttatgg ctcaaacaaa
    21361 catcaaatca accgcactgg aacaaccggt atctatcgcc cgtgacggtt ttatggcatg
    21421 gttttcacaa atgtccactc gttatggctt actgtggtta tgtgtgttat tggtgattat
    21481 tttctcactt accaccgaat ctttcgcctc aatgctgaca ttaaatgcga ttttagaaag
    21541 taaatcgaaa attgccctac ttgctttggc agccacaact acaatgattg tcggcaaaat
    21601 cgacctgaac gtgggtttcg gtattgtgtt gtggcacatt cttgtgatta ctctgcaagt
    21661 ccaatacggc ttctcgtggt atatggcgat cttaatcgtg ttagtgattg ccgctcttta
    21721 cggcttgctg aatgggattt tagttgctct tgctgatatc gatagctttg tggcaacact
    21781 tggctcaggc acagtgcttt atgccgtagc actctggcac tcgggcggtc gccaaattgt
    21841 cggggattta cccgatgaat ttatcgcact caacagcaca gaaattttcg gtatcccgat
    21901 ttctgctttc tatgtgctgg cagtggctat cgtaatgtgg ttggtgacag aacatacccc
    21961 gaccggtcgt tgtatgtatg cggttggtgg caacccaacg gcagctcgcc taaatggtat
    22021 caatattaaa aaatacacca ttgtgccgtt tattgtctct agtgtaatca ctgctttcac
    22081 aggcgtattg atctctgctc aacaaggcgt tggtcaggca agtgtcggta tggactactt
    22141 actgcccgca ttggttgggg cattcttagg gagtaccact attcgcccag ggcgtattaa
    22201 cgtatgggga acagtggtgg ggattgccat tcttgccatc ggtatttcag gtattcaaca
    22261 attcggtgga gcgttctggg ttgagccact ctttaacggt gcaacactgt tactttccat
    22321 cactattgca ggctatgccc aacgcaaacg cttgttaaat caaaaagctg tacagaaaaa
    22381 atcgaccaac taagaggatt tgattatgaa aaaagcattt cgtacaacct taactgcaat
    22441 cgcaacctta actgctattg ctttcggaaa taatgcgttt gccaatgacg acccgttcgt
    22501 acaagaagca aaacagcttg tcgcagcagc tacggctaag caagaaaaat gggacggacc
    22561 gacaaccggc cctcaattac aaaaagagaa aactgtgatt tttattgcct ctgatatgaa
    22621 aaacggtggt gtacttggtg tgattgacgg aatgaaagaa gctgttggcg taacgggctg
    22681 gaagttagat gtattagacg gtgcaggtac ggtaaacaac caactcgccg cacttaacca
    22741 agcgattgcc cgcaaaccgg atgcgattgt gattggtggc tggaacccga atgtggcgaa
    22801 aatcccacta caaaaagcga ctaaaaacgg cattaaactt gcagcttggc acgcaacacc
    22861 ggagccgggt gcgattgata aatacggcat tttctacaac gtgacctctg actcagacga
    22921 aattgcacgc ttatcggcaa tgcttgcggt ggcagaatct aacggtaagg caaaagcgat
    22981 tattttaacc gactcgctat atgaaattgc gttacgcaaa gcgaacgtca tgaaagaagt
    23041 tatcgaaaaa tgtaaagagt gtaaggtgtt agagtttatc gacacgccat tggcggatac
    23101 ctcaagccgt atgcctaacc tcactttcag cttactgcaa aaatatggcg acgatttaga
    23161 gtatgcgttg gcaattaacg atctctactt tgactttatg gctccgtctt tacgcaccgc
    23221 tggtaaagat aaaccgttta gcatttccgc aggtgatggc tcagtaacgg cttatcaacg
    23281 tattcgcaat ggtgataaac agtttgcaac cgtccctgag ccacttaact tacacggctg
    23341 gcagttagtc gatgagctaa accgtgcttt cgctaacgaa aaaccgtctg gttatatcac
    23401 accggctcac ctagtgacta aagaaaatgt ggcgtttgac ggtggtgaaa acaacctgta
    23461 tgccccacaa aacggttaca aaggcgctta caaagcgatt tggggcgtga agtaatgacg
    23521 taggggcaaa taaatatttg cctcgaacaa gcggttgttt tttgcaaaga ttttgcgaaa
    23581 tttaaccgct tgtcataaaa aatagagagg gtaatacaaa tgagcaataa atttatcccc
    23641 aaaatctcca accctcgttt taatgagctg gttgggaata cgtttgaact tgaagagctt
    23701 tttgataagg caatctgggc agaagggccg gcgtggagca agccacaaca agccctctat
    23761 tttagtgatg taaaaggttg cacaatgtac cgctggacag agcaagatgg tacacaggtt
    23821 ttccgccagc cgtcttattt ttctaacggc aatgcgtttg atgcaaacga caacttgatt
    23881 acctgtgaac acggcaatcg aggtatcagt ttaaccacgc caagcggtga atatcgtttt
    23941 ttggtcggtc aattagacgg aaaacgactg aactcaccca atgatgtggt ggtaaaatca
    24001 gacggcatca tttggttcac tgatccacct tacggtattt tgtcggataa cgaaggcaaa
    24061 cagtcgccaa gcgaaattat cggttgttat atttactgct atgatccgaa aaccgacaag
    24121 ctgaatattg caactttcaa cacgatgcga ccaaacggct tggccttctc gtcagacgaa
    24181 aaacaactct ttgtggcaga tatgtcgagc gtggaatttg acgaagatgg cttacaccac
    24241 ttagtcgtat ttgatgtgga cggtaataaa ctaaaaaatc gccgagatgt ggcggaaatt
    24301 agtcccggca ttccggacgg cttccgaatt aaacaaaacg gcgtaatttt ctgctcttgt
    24361 gaagagggaa ttattgctat tttacctgac ggcacggagc tgggacgatt tgtgctgggt
    24421 aagctaacct caaattgtag ctttggcgat gatgaacaaa cactgtttat tacttgttcg
    24481 aatagcgttt atcgtttaac aattaaatag cagaatgcat taagtgcgtc tgttgtagca
    24541 ttacaagcgg tttctttttc aaaatttttt acaaacaaat aatggataac aaaatgaact
    24601 acacactcgg cattgattgc ggtgggactt ttatcaaagc cgctcttttt gatacacaag
    24661 gtaatattca agcccttcat cgtgaaaacg tttcggtaat cagtgaacag gcaggttatg
    24721 cagagcgtga tatgtcacaa ctctgggcta tttgtgcgga agtagtgcgt tcaaccattg
    24781 aaaaaagtgg ggtaaaccca gcggatatta aagcagtagg gatttccgct caaggcaaag
    24841 gggcgttttt actggataag cagaatcgac cgttggggcg tgcgatttta tcttccgacc
    24901 aacgttccct tgagattgtt aaacaatggc aggctgaagg cattccgcaa caactttacc
    24961 cgattacccg ccaaactctt tggacaggac accctgtgtc gattttgaag tgggtgcagc
    25021 aaaatgaacc ggagcgttat gcacaaatcg gggcggtgtt gatgtcgcac gactatttgc
    25081 gtttttgctt aaccggtgag ttgcattgcg aagagaccaa catttccgaa agcaatcttt
    25141 acaatatgga aaaaggcgaa tacgaccaac agttagccga tttgttggga atgccggatg
    25201 tgttggagaa attaccacca gtgattgaac cgaataaaat ttcgggatat gtgactgaaa
    25261 atgccgccaa attaaccggt ttagcggtgg gtacgccgat agtcggtggc ttgtttgatg
    25321 tggtttctac cgcacgttgt gccaatttag atgacgaaac caaactgaat gcagtgctgg
    25381 gaacgtggtc ggtggtgagc ggcattaccg atcatattga tgcaagccaa tcattacctt
    25441 ttgtttatgg gcgttatgcc gaggcaggga aatttatcgt ccacgaagca agcccgactt
    25501 ctgccggtaa tttagagtgg tttgtgaatc agtggaaact cgattatgtg aaaatcaacc
    25561 aagctgttgc ccaattaccg ccggcggcaa gctcggtgct gtttgtgccg tttctgtatg
    25621 gctcaaatgc aggcttagga atgcaagccg gtttctatgg tatgcaagcc caccataccc
    25681 aagcccatct cttgcaggca atttacgaag gagtgttgtt tagcttgatg agccatttaa
    25741 accgtatgtt tatccgcttt ccaaaggctg acgtgctgcg agttaccggc ggaccggtga
    25801 aatctaaagt ttggatgcaa atgttggccg atttaaccgg aatgcggctt gaaataccac
    25861 aaatggaaga aaccggctgc ttgggtgcgg cattaatggc aatgcaaggg gttgggctgg
    25921 atataagcca agttcaagcc ctaaataatg aaatgcaggt gtttgagcca aatccggaaa
    25981 attatgcggc ttatcagacg aaatatcagc gttaccaaaa attaactgaa gcattaaata
    26041 caatgattta acctgaattt atgcttttac aagcggtaat atttggttac ttttttgcaa
    26101 aaattactgc ttttttgttt atttaattta tgaatttaat taaata
//
LOCUS       DANLXQ010000017        24168 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.017, whole genome shotgun sequence.
ACCESSION   DANLXQ010000017 DANLXQ010000000
VERSION     DANLXQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24168
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.017"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            3..236
                     /locus_tag="PXD94_000544"
     CDS             3..236
                     /locus_tag="PXD94_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL6285438.1"
                     /translation="MEELKAYLNSLTTKEQESFALRCNTTVGYLRKSISRNSRLGAEI
                     CVAIERETKGLVTRKHLIEDWHLRWPELANDKQ"
     gene            393..635
                     /locus_tag="PXD94_000545"
     CDS             393..635
                     /locus_tag="PXD94_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6285439.1"
                     /translation="MYGINLNLNEKSSQTQNARILAHLQNGGRITSLSALDDFGCLRL
                     SARIKDLRDRGHDIQSVFITVPNGKKVKQYFMECAK"
     gene            632..983
                     /locus_tag="PXD94_000546"
                     /pseudo
     CDS             632..983
                     /locus_tag="PXD94_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1121..1405
                     /locus_tag="PXD94_000547"
     CDS             1121..1405
                     /locus_tag="PXD94_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285440.1"
                     /translation="MLLEQFGITGKLAEDFIVHRKAKKAPITETALNGYQREADKAKI
                     PIQKAVEIAIERGWTGFKADWQWQDDQPKHRPKDNMRAEWNNPEAWAEVF"
     gene            1405..2052
                     /locus_tag="PXD94_000548"
     CDS             1405..2052
                     /locus_tag="PXD94_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL6285441.1"
                     /translation="MSLQKTEQNRPLAVLGEQQGKLSGEVESLVDRIFDQLLASCPSI
                     QYWSEKQVATAKKQWILGFAENGIRTVEQVRQGMKALRAKTDDFVPSIGKFIQWCNTI
                     DYHELGLPDLEQLLKLLDRSLRFDFIEIQKLKFKSNAEYWLITDLYARNKKYSWKDET
                     LRNQAEKALVAMAKRIMSGEQIPPPKPLIEEKKKVPIDPRIQRILDEKKQAGAMQ"
     gene            2049..2621
                     /locus_tag="PXD94_000549"
     CDS             2049..2621
                     /locus_tag="PXD94_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL6285442.1"
                     /translation="MTDFDKDTYQTPKYFRNWLNHRYAWFHIDGCSNGKNALYSYWIG
                     KAAEGLDEDTLSGQIAEDFLAENLLDVILERVADWGELLRIFVNPPYSNPLPFVQRAA
                     ELKKAGHIVVMLLPADKSTRWYQVIQENASEVIDIIGGRINFINPLTGEEVKGNNKGS
                     MVAVFDPFVEGIVTRQMPLDKIKELGGYEK"
     gene            2740..3192
                     /locus_tag="PXD94_000550"
     CDS             2740..3192
                     /locus_tag="PXD94_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL6285443.1"
                     /translation="MAKYQMMKLPGGILSPLNDEEAERLKSFQNGEQYEIEIKRQRNP
                     AFHRKVFAFFNFCFDCWSGSHSDWEFQDPTAQFDTFRKHLTVMAGFYVQTWNLKGQVR
                     IEAKSLSYANMEQDEFEQAYKALINAAIKHVFNNTKDENTINQLYSFF"
     gene            3242..3454
                     /locus_tag="PXD94_000551"
     CDS             3242..3454
                     /locus_tag="PXD94_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285444.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLISLSILTLRYIGYLLIYPLAFFVA
                     VGSFIVIGQWKYQECLDDFKSDFNPI"
     gene            3831..4115
                     /locus_tag="PXD94_000552"
     CDS             3831..4115
                     /locus_tag="PXD94_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDL6285445.1"
                     /translation="MANLRKEAKGRECQVRLPGICNHNPETVVLAHYRMAGLNGVGMK
                     PDDIFGAWACSSCHDECDRRTRKLEADYVRQAHAEGVFRTQAILRREGKL"
     gene            4112..4480
                     /locus_tag="PXD94_000553"
     CDS             4112..4480
                     /locus_tag="PXD94_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL6285446.1"
                     /translation="MSDWLEIALPYPPSVNHYWKHTRNGRHYISDKGKAFRAEVVKIC
                     KMFDPFKGAVQTVLQIYYPDKRKRDPDNLEKALWDALKIGGLIEEDDNQILVDKRTIT
                     AGFKKGGMVVLKIKAVGNAN"
     gene            4470..4835
                     /locus_tag="PXD94_000554"
     CDS             4470..4835
                     /locus_tag="PXD94_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285447.1"
                     /translation="MQIDVKGVLNLWGGYVRFTDCKGYPTMQAFMRESPQERTKQRYL
                     ARLDDDTLEKVDRQMRKLQANDKRQFDVLFYKYAVGLENKQVWQKLSLSKTSYHTELL
                     LAERFMEGALAGSEIRLLL"
     gene            complement(4803..4997)
                     /locus_tag="PXD94_000555"
     CDS             complement(4803..4997)
                     /locus_tag="PXD94_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285448.1"
                     /translation="MALSRTEIVNRSNAKRGIKLKAFKLHESTIAQIEQLAQQLNIPQ
                     NQLIKLAVEKLQEQTNFTTG"
     gene            5424..5499
                     /locus_tag="PXD94_000556"
     tRNA            5424..5499
                     /locus_tag="PXD94_000556"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5457..5459,aa:Trp,seq:cca)
     gene            complement(5514..6092)
                     /locus_tag="PXD94_000557"
     CDS             complement(5514..6092)
                     /locus_tag="PXD94_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285449.1"
                     /translation="MIEFDQIDDSFYHFPFRPRPGDTSSDTATPCIGGVDLVSNPEKV
                     ELIPEAAHSPMLKKLLTDLNTPNSKLLTLGCAYWAHKDNRDTSYAYLEFSFREHSVAT
                     NLEFIRSIDEQFEQFLQENKKQLSIEFSVPEQAFDIVSQALFWSIRPFSYFGSEERIL
                     IYFQAGSPRHQDLEIFLDLLHRFLTEYLVVPA"
     gene            6211..6555
                     /locus_tag="PXD94_000558"
     CDS             6211..6555
                     /locus_tag="PXD94_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDL6285450.1"
                     /translation="MPEKNPDIYTQAWNYLVMFLSNHNGWICGVIIAFFTSITKSFLY
                     GKTDTPKRVLAEAILCSVIAGSMRPILIYLNADVSLITPIGAGIGLLGTSAIRQMILK
                     FLNKKAGGENVD"
     gene            6545..7138
                     /locus_tag="PXD94_000559"
     CDS             6545..7138
                     /locus_tag="PXD94_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDL6285451.1"
                     /translation="MWISESKFKQVFPNAVSGIYSIIDKHIEKAGCKTKEQQAMFLAQ
                     CGVECAGYSVFEENLNYSATALLRTFPKYFSQHNVVLYARDKRAIANRVYANRMGNGN
                     EQSGDGWKYRGRGLIQITGKANYIEFAKWEGLPSLVTDPDQITKNLALTVQVAVWYWV
                     SRKLSEFSDILTVTKKINGGTHGLTQRTAYYKKLMAD"
     gene            7139..7468
                     /locus_tag="PXD94_000560"
     CDS             7139..7468
                     /locus_tag="PXD94_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL6285452.1"
                     /translation="MIKYIYLAATVMILGLCGWIWHQSKQIDGLRAENQTQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLH"
     gene            7434..7667
                     /locus_tag="PXD94_000561"
     CDS             7434..7667
                     /locus_tag="PXD94_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285453.1"
                     /translation="MVLLIALSGCTSKTIVSTEYLYPPAAYLVPCERTAFSGKTYGDT
                     VDYLIKVMGERDLCASQIDRIREWQAQTKQGFK"
     gene            7934..8410
                     /locus_tag="PXD94_000562"
     CDS             7934..8410
                     /locus_tag="PXD94_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDL6285454.1"
                     /translation="MDNLFDLKLNINQIAELTGLHRQTVSQRVAGLNPALGSNSKLKL
                     YALRDLILTGLAEKMSADVDSLSPADRRAWFQSENERLKFEKEIGELIPASEVALEMS
                     ALAKTVVQALETLPDILERDCGLQPKDLIRVQQVTDDVRDQMALHIQEVTTDTENE"
     gene            8410..10521
                     /locus_tag="PXD94_000563"
     CDS             8410..10521
                     /locus_tag="PXD94_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDL6285455.1"
                     /translation="MFASAKEIRRDIANAIKAPRRMRVSEAVAEYMRVPLGGGNSVKW
                     DKHTAAYMLEPMDCLNSREYDAVIFVGPARTGKTIGLIDGWITYSIICDPSDFLLVQL
                     TQEKASEHSRKRLDRTFRCSPEIAKRLSPRKNDNNVHDKYFRAGNLLKIGWPSINVLS
                     SSDYKYVALTDYDRWPEDVDGEGDGFSLASKRTTTFMSAGMTLVESSPGKDIVDLKHI
                     PKSTHEAPPTTGILSLYNRGDRRRFYWQCPDCSEYFEPSMANMVGYREDSDFVKASEN
                     ARLQCPHCQTLIAPELKRELNIKGVWLKEGQKIDASGQISGESRKSRIASFWLEGPAA
                     AYQTWAQLTYKLLNAEHEYEMTGSEETLKAVTNTDWGLPYLPRSALEQRRSDELMERR
                     EEVEEKTVPAQCRFIVAAVDVQGGKNRRFVVQMVGYGENGERWLIDRYNISHTLPDSD
                     GVIEKIDPRIPDDWYILISDVLEKRYPLAHNPNHFMPILAMAVDSGGEDGVTDNAYQF
                     WRKCRRDGYAKRVYLVKGDSTKRQKLITKTYPDNTARSDRHSSARGDVPLYLLQTDYL
                     KDRINNALFRDTVGANYIHFPDWLGEWFFNELTYEERGADGKWRKPGKGNNEAFDLFC
                     YAHAIAILRGYERIKWGDENDVPSWAKLPDINPNIIRETPRTAEQAVEIEEKSEKPQP
                     KPTKAKSSWLSGGGRKTGGWL"
     gene            10518..10742
                     /locus_tag="PXD94_000564"
     CDS             10518..10742
                     /locus_tag="PXD94_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285456.1"
                     /translation="MSLYTIDELKQKIRTLDEKIETAQSQVSFNGRSVSYQVAELTKQ
                     RDRYQTMLDEQLAQSGQRTKQHRIKYARFI"
     gene            10742..12241
                     /locus_tag="PXD94_000565"
     CDS             10742..12241
                     /locus_tag="PXD94_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL6285457.1"
                     /translation="MNFLEKTIATLSPKWAANRSRNRYVLNAYEAAQPSRTHKASRES
                     KGANSTVRQSAVSLREQARALDQNHDIVIGILDKMEERVIGSKGIHIEPQPLTLAGEV
                     HEELAGQIRKLWAEWSVKPDVTGLYTRPLLERMLLRTWLRDGEVFVQLVKGKVAGLEH
                     GSQVAFSLEALEPDFVPMQSDEAKNGLVQGVFLNAWRKPTAYQVYLDNPQESNGMYGK
                     VKTVPADNMLHLAFRKRLHQIRGVSMLHGVIVRLADLKEYEESERVAARIAAAMTMYI
                     KKGDAALYDDDSNSDGERLFDIAPGAVIDDLKPGEDIGLINSNRPNVNLETFRNGQLR
                     ATAAGTRSSYSSIARDYNGTYSAQRQELVESFEGYAVLQDAFVAAISRPIYREWLKMA
                     IASQAIKLPPDIDQNSLFNAVYSGPVMPWIDPIKEANAWKERIKGGLATEGQAIRASG
                     NNPAEVKRQRIVEIKENQAEGLKFDTDLTNYGGQDKSFSNQKDEINEDE"
     gene            12253..14214
                     /locus_tag="PXD94_000566"
     CDS             12253..14214
                     /locus_tag="PXD94_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDL6285458.1"
                     /translation="MKTWFNIQAKANDTAEISIFDEIGFWGVTAQQFAKDLKALGNNL
                     KQINLHIHSPGGDVFDGIAIYNLLKNHPANKTVYIDGLAASMASVIAMAGDEIIMPEN
                     AMMMIHKPWGIQGGDADDMRKYADLLDKVESTLIMAYVAKTGKYETDLAEMLKEETWL
                     TGKECVEQGFADKLAEPLVAMACIQSKKLEDYTKMPDKIKNMLFAPQGNAGANPKHEQ
                     PQTPPQAGENPQNVAKPDAMVALAQRNATIKATFAAFGTQFDGLLAECLADVSMTAEQ
                     AKDKLLAKLGENTTPSVPQNHIHAGNGNIVGDSVKQSLMARAGQDTDKTNARDNAYNA
                     MTLRELARASLVDRGVGIAGYTPMQMVGLAFTHSSSDFGQILIDVAHKSLLKGWETAT
                     ENYEQFTTRGTLTDFRPAKRVGLGEFGYLPEVGEGEEYTYGTIGDEGASVALATYGQL
                     FSITRQAIINDDMHLLTKIPEKMGAAAKATITKLVFALITGNATAQDGKKLFDATHKN
                     SLTGAALNVENIDKAIQLMNGFVNSRGEPLSIEPEFMLLPTSLHTKAKQILGSSSVEG
                     ADINSGIINPIRDIVSPIKSPRLQIADPKSWYLLNKEAIEVSYLDGVDSPYIEQQHGF
                     TVDGVSTKVRIDAGVNVIDYRGVVKVTNA"
     gene            14288..14611
                     /locus_tag="PXD94_000567"
     CDS             14288..14611
                     /locus_tag="PXD94_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDL6285459.1"
                     /translation="MAKNYIQDGNTVRLTATKAITSGDVIVAEDLIAIAVSDAAKNDA
                     VVGLTTGVFSVKAKQADDIKQGAVLYWSETEGATLTAGSNKRLGIAWKDSGTSSNQVD
                     VKINV"
     gene            14604..14906
                     /locus_tag="PXD94_000568"
     CDS             14604..14906
                     /locus_tag="PXD94_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285460.1"
                     /translation="MSSPFEQAIAAADQTIQQTMMSEWLIGGKPYPATFDEAPAIFNG
                     LHSTDDRAIHGTERTLTLFRASGYKPRLDDRVSGNGKKYLVKSYHFVDQLIVLQLE"
     gene            14910..15434
                     /locus_tag="PXD94_000569"
     CDS             14910..15434
                     /locus_tag="PXD94_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285461.1"
                     /translation="MGSQVTGLKELQASLKKIARQTVPKAAAQAIRTVGRQAMNKAVK
                     SVAAEIGVNQKTIKGRAKMTAKPTPSRLQATIKVNRSHMPMIRVLERKSNRLSPTKGS
                     IRVGKHTVQRGFRQRLANGRTHIMYRQGRKRYGIDVAKVPLSQPLTQAFDRELKNYPE
                     QVQAELAKQIAGKL"
     gene            15431..15826
                     /locus_tag="PXD94_000570"
     CDS             15431..15826
                     /locus_tag="PXD94_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285462.1"
                     /translation="MKIHTKIRKEVLELLQTKLPEIDNFYNGLPTFIDIEEEQLAVSV
                     YLDDISLQEITVCNNQWDAKLNITIHLKSTESAEDELDEWAEKIREIIDDYPEFEHLE
                     SVALSQYQYEQDQNQRTWHMATLIFDVEY"
     gene            15854..16495
                     /locus_tag="PXD94_000571"
     CDS             15854..16495
                     /locus_tag="PXD94_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285463.1"
                     /translation="MAKTTKVQGTKFRISVGREAQKAITAINLNTATLTIASSGYKKG
                     DAIELTGCGQLDGIYPVLSVTTDQVKLCEEVNWTGKDLPANYTKAKAALVQYSEQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPTTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            16579..16980
                     /locus_tag="PXD94_000572"
     CDS             16579..16980
                     /locus_tag="PXD94_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285464.1"
                     /translation="MTLREKLLANKPKLQPIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGVLLFNPLDINDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            17025..17255
                     /locus_tag="PXD94_000573"
     CDS             17025..17255
                     /locus_tag="PXD94_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL6285465.1"
                     /translation="MPERHFAEYRLFYQEQPFGLWRDDYRTAQLAHLTAMFNRDPKGK
                     APKLAEFMPFFNRTDEVEEIEDDGVADYLAKR"
     gene            17318..17545
                     /locus_tag="PXD94_000574"
     CDS             17318..17545
                     /locus_tag="PXD94_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285466.1"
                     /translation="MFEALDRFMINTIKIAIIVFLVACIFISMPNWWIASILVLFPTA
                     YLLDKYWWRNTEMYRRKMDKLVEEMKNNRQI"
     gene            17599..21150
                     /locus_tag="PXD94_000575"
     CDS             17599..21150
                     /locus_tag="PXD94_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6285467.1"
                     /translation="MSSSNRLTITLDVENSKLLAGFAQSDTVANKFARSFIVNMDQAQ
                     QKARQFADRSTKYLKNIEQAANNLNSSVSKERFWQIANRLQFPTSKVISYANSYTELG
                     NKLKLVTESESQHARAMAAVYDISLKTAQSTQATSSVYQTFAQNAKQLGISQNDVAKL
                     TETVAKSVAISGASSATASNALVQFSQSLLMGKLKAQEFNSLMTQTPSVIQAIAKGLG
                     ITTAELKAMVDNGEMSAEKMIEGLKKAESYVNGQYAKTTTTISGAMQNLSTAAEKWVG
                     EMDSTVGASQKAVAVLNALNAHFDTFAKTALVAGGAFVLFKGYMSVRPFIEARAAIYE
                     NRKAIIAENQARQQTIATRQQELVVLSQQTALKLEQTLQTYNQNKAELEAIAVKQQKI
                     TKEREEIAVAIQAAATRTERLALSAELQALDQKEIALGQQKIAVERQQIATKQALKVA
                     YAENAVAQSAMNAGMVTATTTTRAMNGVLAAARAELNLLKAAALSNPLMTLAMVATTA
                     GTALWGFFESQKAAREEALRYADSLDSVRANIEKMTKAQTDAEMVKLSRSIKEQETAL
                     ASLKQRQAELTREIEQGATVLDDAFGAGVRFEKSAKELAKAQEELKLVTADVEKAQNT
                     LNASLEAQEALQAHVPIANLREEFVKLYPHIDESQIKVDGLNIAIGNFTVKSPEMVIA
                     ANNIANALGGVAGEAMRAAILVANLSSMKLDANGAAIIDPKHLEKIEQIERNNAIASA
                     KGKDKIALQVKDALIKSGMKEGDAGYERLKAAYEQQFTLQNAPKRGGKPKKTKAERSA
                     ESYQNQVAEMTNRLSGLKIDKEDLVKYGGISQYQEVRKLTEDIALNAEKYKNYGTEGV
                     AKLKELAAKIDSEQQLLDIEQFQVDHDKKLEAMQFELDLLGKTRTEKELLQFNNQLDL
                     EAERLKINMTKENIALLDQKIAKIKEEYELHQKKQEETRASVMMGIKEGWNNIETDVS
                     NVAANVANITQNTFDGMADSLTNFVMTGKADFRSFANSILSDLSKMIIKMMLFNAIKQ
                     GATMLFGYQGPTTTVNGNAVYGLGTWATGGYTGNGGKYTPAGIVHKGEYVITKEATSR
                     LGLDYLNYLNYGRRGFASGGGVAVPRVPSSSYQPKSAQSSISVQVINNGEPVDAKVSQ
                     KQQGEQTQITVELMRKIARQEANGMIQNNFRAGGVFA"
     gene            21150..21479
                     /locus_tag="PXD94_000576"
     CDS             21150..21479
                     /locus_tag="PXD94_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285468.1"
                     /translation="METLQWCVRTDLAVENTPEIMEVKFGDGYTQRSPKGLNNLLRAY
                     TGTIKVKKGEHLAVEAFFAKHRGVSPFYFKDPYTQQNKKVVCSSWPAKMVGLNHWEFS
                     VTFKEVP"
     gene            21479..22195
                     /locus_tag="PXD94_000577"
     CDS             21479..22195
                     /locus_tag="PXD94_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6285469.1"
                     /translation="MPVSISNQMKLDLAKLEQNAMLDLYEVDLRTLKDKSGNAGSVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEASGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKATSDPKADKCSHCLNG
                     CELRGNQRNFGGFVSVNKLG"
     gene            22199..22930
                     /locus_tag="PXD94_000578"
     CDS             22199..22930
                     /locus_tag="PXD94_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6285470.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEEKGELVAVVHSHPEPNGKPILSVLDRKMQVQTGLDWWLVHNRQIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFDRQDDWWHTGQNLYLDNIQG
                     QGFERVGTPQIGDVILMQVGADVPNHAAIYIGEQMVIHHSPNRLSKRDLYDGYWLRHT
                     HSIWRHKLADKLDFDGILNDIAVNN"
     gene            23199..23789
                     /locus_tag="PXD94_000579"
     CDS             23199..23789
                     /locus_tag="PXD94_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL6285471.1"
                     /translation="MITVKFYGHLRKFGTDFKLEVKDTAEAIRALCTQIRGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFQAVLGVALIGAA
                     FFLGPVGWGVLGASSAMMMGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETMTVEV"
     gene            23792..>24168
                     /locus_tag="PXD94_000580"
     CDS             23792..>24168
                     /locus_tag="PXD94_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285472.1"
                     /translation="MGLFSRKRKNHTPKEAPETGRSKQIVNIVELLCEGEIEGLVDGF
                     KSIYLDGTKIQNDDDSYNFNNVSGQLNVGTQDQEVLEGYDSSQNEVSVGVEVKKKNGA
                     IVRTVTDERISRLRLTLGVRSLF"
BASE COUNT         7517 a         4817 c         5657 g         6177 t
ORIGIN      
        1 caatggaaga attaaaagca tacctcaatt ctttaaccac aaaagagcaa gagagttttg
       61 ccttgcgttg taatacgaca gttgggtatt taagaaaatc gatctcaaga aattctcgat
      121 taggtgcgga aatttgcgtt gcgatagagc gtgaaacaaa aggtttagtt actagaaagc
      181 atttaattga agattggcac ttacgctggc cggaattggc aaatgacaag caataaaggg
      241 aaagtttttt ctaaaaaact caaatcattc taccacagta aaaattcaat ttttattaga
      301 gtggcaattt ttatgctgct tgcgtggtgg gtttttaaca attaaaaaaa ccaccgctgt
      361 aacggtggct aattactaag gaggtttcct atatgtatgg gataaatctt aacctaaacg
      421 aaaaatcaag tcaaacccaa aatgcaagaa ttttggcaca tcttcaaaat ggcggtcgta
      481 ttacttcgct ttctgcgtta gacgatttcg gctgcttgcg tttatccgct cgcattaaag
      541 atttgcgtga ccgtggtcac gatattcaaa gcgtatttat tacagtgccg aatggcaaga
      601 aagtgaagca gtattttatg gagtgtgcga aatgagattt agcacttaca tcaacaatgc
      661 aaaatgcctt gaatggaact taaatgctaa tcaaggagca ttatttgatt tactgaatca
      721 agctcctgct tgggcgaaag aaatcattat tgatggcgtg gtttattact gggtttcccg
      781 taatgcggtg atcgaagaac tacctttgtt ttactcaaag cctgataccg tctatcgcca
      841 ttttgtcgag tttcacaaaa aagggttgat cgtgtatcaa aaacacggag ataaagacct
      901 tattcgatta actgaaaaag ggaagacttg gaatgagttt aactcggatt taaatccgac
      961 actcggaaat aaatccgagt tagctcggaa acagatccga aaaagctcgg atttaaatcc
     1021 gacaaataat aatactaatt ataaaaatac taatgatcat aataataccc ttacgggtat
     1081 taacgcacac acacgtgaga ctaaaaaatc tgctgtgttg gtgttgcttg aacaattcgg
     1141 cattacaggc aaattggcgg aggattttat cgtacaccgc aaagccaaaa aagccccgat
     1201 taccgaaact gcactgaatg gctatcagcg agaggcagac aaagccaaaa tcccgattca
     1261 aaaggcggta gaaattgcga ttgaacgtgg ctggacagga tttaaagccg attggcagtg
     1321 gcaagacgat caacctaaac atcgcccaaa agacaatatg cgagctgaat ggaataaccc
     1381 tgaagcgtgg gcggaggtgt tttgatgagc ctgcaaaaaa cagagcaaaa tcgaccgctt
     1441 gcggtgttag gtgagcagca aggcaaatta tccggtgagg ttgaaagcct tgtggacaga
     1501 atatttgacc agttgcttgc gagttgccca agtattcaat actggtcgga aaagcaagtt
     1561 gccacagcca aaaaacaatg gattttaggc tttgcggaga acggaatccg cacggttgag
     1621 caagttcgac agggtatgaa agcgttaaga gcaaaaactg atgatttcgt cccgagtatc
     1681 ggcaaattta tccagtggtg caatacgatt gactatcacg aattaggatt acctgattta
     1741 gagcagttac tcaagctttt ggatcgctct ttaagatttg attttattga gattcagaaa
     1801 cttaaattta aatcaaacgc tgaatattgg ttgataactg acctatacgc acgtaacaag
     1861 aaatatagtt ggaaagatga aaccttacgt aatcaagcag aaaaagcctt agtggcaatg
     1921 gcaaaacgaa ttatgagcgg agagcaaatt ccaccaccta aaccgctaat cgaagagaaa
     1981 aagaaagtgc caatagatcc acgcattcag cgaattttgg acgagaagaa acaagcagga
     2041 gcaatgcaat gacagatttt gataaagaca cttaccaaac gccaaagtat ttccgtaatt
     2101 ggctaaatca tcgctatgcg tggtttcaca ttgacggttg ttctaacggc aaaaacgcat
     2161 tgtatagcta ctggattggc aaagcggcag aaggcttgga cgaagacacg ctaagtggtc
     2221 agattgccga ggatttcttg gcagaaaact tgctcgatgt gatcttggaa cgtgtggcag
     2281 attggggcga attactccgt atcttcgtta atccgccata tagcaatcca ctcccttttg
     2341 ttcagcgtgc tgcggagctg aaaaaagccg gtcatattgt ggtaatgctg ttgccggcag
     2401 acaaatctac tcgttggtat caggttatcc aagaaaacgc tagtgaggtg atcgacatta
     2461 tcggtgggcg tattaatttt attaatccgc tcacaggcga ggaagtgaaa ggcaacaaca
     2521 aaggctcgat ggtagcggtg ttcgacccgt ttgtggaggg aatagttacc cgtcaaatgc
     2581 cgctggataa gattaaggag ctgggcggct atgaaaaata acgaagggtg ggaagtgtga
     2641 tgagtgattc tactattgga tttttaggga ttctaatttt cttcgggtgg ttagcatatt
     2701 tggtatttag agcttttgga ttttcattga gggatagaga tggctaaata ccaaatgatg
     2761 aaattgccgg gtgggatttt aagcccgtta aacgatgaag aagccgagcg gttgaaatcg
     2821 tttcagaatg gcgagcagta cgaaatcgag attaaacgcc agcgtaatcc cgcttttcat
     2881 cgcaaggtgt ttgcgttctt taatttctgt tttgattgct ggagtggtag ccattccgat
     2941 tgggagttcc aagatccaac cgctcaattt gatactttcc gcaagcattt aactgtaatg
     3001 gcagggtttt atgtgcagac gtggaattta aaagggcaag tgcggattga ggcgaaaagc
     3061 ctaagctacg ccaatatgga acaagacgag ttcgagcagg cttataaagc gttgattaat
     3121 gcagctatca agcacgtttt taacaatacc aaagatgaga atacgataaa tcagttgtat
     3181 tcgttttttt aattaaggcg aaatcgccat atttaattag gtttaattgc aggagattaa
     3241 aatgaaaaag aatgttatta gatattggct tagcgtgcca ttcagaatta ttgcagtgct
     3301 tatttctttg tcaattctta ctcttagata tatcgggtac ttattgattt atccattagc
     3361 attttttgtg gcagtcggta gttttattgt tatcggtcaa tggaaatatc aagaatgcct
     3421 tgatgacttt aagagcgatt ttaatccgat ttagtgtgtg tttattaatt ccctcgaatt
     3481 cgggggtagg agaaatcaaa atgacaaaaa ttctttgaaa ctatcgcact cgtgatgtta
     3541 tttggcatta ttccaagctg tttattggcg attaatacta ctttaatgaa tattaaaaac
     3601 gcaccgtggt ttatggtgat tattcagtta gggttttgga ttttaatatc gtttgttatc
     3661 ggttgcggat tattcctttt atgtggaatg ttatttaatt taaggagatt aataagtgag
     3721 ccagtgcaat gaactgactg taattttaat cactatcgct attcttagtg tatgtgccgg
     3781 ttcactcttt tttattgagt ggtttgataa aagagaccaa ggaggttgga atggctaatc
     3841 tacgcaaaga ggcaaaaggc agagagtgcc aagtccgttt accgggtatc tgtaatcata
     3901 atccggaaac ggtggtattg gctcactatc gaatggcagg attaaacggt gtgggaatga
     3961 aacccgatga tattttcggg gcgtgggctt gcagtagctg ccacgatgag tgcgaccgta
     4021 gaactcgcaa attagaagcg gattatgtga gacaggctca tgcagagggc gtatttagaa
     4081 cgcaggctat tttacggaga gaagggaaat tatgagtgat tggcttgaaa ttgcgttgcc
     4141 ttatccgccg agtgtgaacc attactggaa gcatacccga aacggtaggc attatatttc
     4201 agataagggg aaagccttta gggcggaagt ggtgaaaatc tgcaaaatgt ttgatccgtt
     4261 taaaggagcg gtacaaacgg tattgcagat ttactatcct gataaacgca agcgtgatcc
     4321 agataattta gaaaaagcct tgtgggacgc attaaaaatc ggtggcttaa ttgaagaaga
     4381 cgataatcaa attttagtcg ataagcgaac cattacggca ggttttaaga aagggggaat
     4441 ggtggtgtta aagattaagg cggtgggtaa tgcaaattga tgtaaagggt gtattaaatt
     4501 tatggggtgg atatgtgcgt tttaccgact gcaagggcta tccgacaatg caggcattta
     4561 tgcgtgagtc tccacaagag agaacaaaac agcgttattt agcccgctta gatgatgata
     4621 cgcttgagaa agtcgatcgg caaatgcgga agctacaagc gaacgacaag cgacaattcg
     4681 atgtactatt ttataaatat gcagtagggt tagagaataa gcaggtttgg cagaagttat
     4741 cgctcagcaa gacttcatat catacagaat tacttcttgc tgaacgattt atggaggggg
     4801 cattagccgg tagtgaaatt cgtttgctct tgtagcttct caacggctaa tttaattagt
     4861 tgattttggg gaatatttaa ctgttgggca agttgctcga tttgtgcgat tgtgctttcg
     4921 tgcagtttaa aggctttaag tttaattccc cgttttgcgt tgctacggtt tacaatttcg
     4981 gttcttgaca gtgccatcat aaatccttaa tataggtttt gaagagtagg gagatttctc
     5041 cccctactta gtttcattca attagtatgc tggcaggcta atcactaaga gaatgataac
     5101 taggataatg cggtaaagca tttgttatcc ctccaaaagt cggtgggatt aaaggtcgcc
     5161 accggctcgt ttccagcact attactgaaa acaagtttat tatatgttgg ataaccaata
     5221 tattcaagca aaatatcgtt aaaaagccca tggttttgta aattatgggc ttttttgcat
     5281 ttaaatgatt aaaaagtgtt gcaaagttcg gacttaatct atattattta tggtaagttg
     5341 cggttcattg cataggtggt gaacttaaaa gaattttaca gccctgagca gaaatgcttg
     5401 gggctttttt gtttacggat tttaagggtg tagctcaata ggtagagcaa cggtctccaa
     5461 aatcgttggt tgttggtttg agtccaacca ccctttccaa atgcgagtaa atgttatgct
     5521 ggaacaacta aatactcagt caaaaagcgg tgtagtaaat ccaaaaagat ttctaaatcc
     5581 tgatgtcgag gactaccagc ctgaaagtat atcaagatgc gctcttcgct tccgaagtaa
     5641 gaaaatggac gaatactcca gaaaagagct tgtgaaacta tatcaaatgc ttgctcaggc
     5701 acggaaaatt caatagataa ctgcttttta ttttcttgca agaattgctc gaactgttca
     5761 tcaatacttc tgataaattc taggttagtt gcaactgaat gttcccggaa tgaaaactct
     5821 aagtaagcgt atgatgtatc tctattgtct ttatgagccc aataagcaca gcctagtgtt
     5881 agaagtttgc tgtttggagt gttcaagtct gtgagtaact tttttaacat tggggagtgt
     5941 gcggcttctg gtattagttc taccttttca ggattggaaa caagatctac tccaccaata
     6001 caaggtgtgg cagtgtcgct tgaagtgtcg cccggacgtg gtctaaatgg gaagtgatag
     6061 aaactatcgt caatttgatc aaattcaatc atttttaacc tctgctagtt tatttgttgg
     6121 ggaacagtat tctagcagaa tttttaacca aggctcagtt ttacgactgg gcttttttat
     6181 tgcctcgaaa cggggtggag tatgaataca atgcctgaaa agaaccctga tatatatacg
     6241 caggcttgga attatcttgt aatgttttta agcaatcata atggctggat ttgtggcgtt
     6301 atcattgctt tttttacatc tattaccaag tcatttctgt atggtaaaac agatacacct
     6361 aaacgagtgc tggcagaggc tatcttgtgt agcgttattg ctggctcaat gcgtcctatt
     6421 ttaatttatc tcaatgctga tgtatcactg attaccccta ttggtgcggg aattggatta
     6481 ttgggtacaa gtgcaattcg ccagatgatt ttaaaattct tgaacaaaaa agcaggtggt
     6541 gaaaatgtgg attagtgaaa gtaagtttaa acaagtattc cctaatgcag ttagtggtat
     6601 ttactcaatt attgacaaac acattgaaaa agcaggctgc aaaacaaaag agcaacaagc
     6661 gatgttcttg gctcagtgtg gtgtagaatg tgccggatat agtgtatttg aagaaaacct
     6721 taactattct gcaactgctc tattgcgaac attcccgaaa tactttagcc aacacaatgt
     6781 cgtactttat gctcgagata agcgtgctat tgctaatcgg gtgtatgcaa atcgaatggg
     6841 taacggcaat gaacaaagtg gcgatggttg gaaatatcgt gggcgtggct taattcaaat
     6901 taccggtaag gcaaattata tcgagtttgc taaatgggaa ggcttaccaa gtctagtgac
     6961 tgaccccgac caaatcacta aaaatctagc attgacagta caggtggctg tttggtactg
     7021 ggtaagtcgc aagttatccg aatttagcga cattcttact gttactaaaa agattaatgg
     7081 cggaacgcac ggtctaactc agcgtacagc ttattataaa aagctaatgg cggactaaat
     7141 gattaaatac atttatctag ctgccacagt aatgattttg ggtttgtgtg gctggatttg
     7201 gcaccagtcc aagcagattg atggcttaag agccgaaaac caaacacaag cccaaaccat
     7261 tgagcagcag caaaaagcca atcaacggct aaccgatagc ttagagcaag agcgacaagc
     7321 ggtcgaaaaa atccaaaaaa ttgcaaatga gctgcggaat aaagtggaga tggccaagaa
     7381 tgaaattaca tcaatactgg cacaagacag ttgtgctaag gctgatttgc ctaatggtgt
     7441 tgctgatagc attaagcggt tgcactagca aaacaatcgt cagcactgag tatttatatc
     7501 cgcctgcggc ttaccttgtg ccgtgcgagc ggacagcatt tagcggtaaa acctacggcg
     7561 atacggtgga ttatctcatt aaagtaatgg gagagcgtga tttgtgtgcg agtcaaattg
     7621 accgtattag agagtggcaa gcacagacta agcaagggtt taaataactt ggcggattaa
     7681 accgctaatg agagtgggag cttaattgct cccttttcat ttgcgttaca ttacttagtt
     7741 atgcttttga ttgtatcaag atcataacta agcaatgtgt tgcatagttt gtaaaaggta
     7801 ctcccgaggg gatacccctt tccacggggt tgcggcagcg tggttttcgg caattttttg
     7861 agtttctagg catcatcatc ttcctatgat gttgctgttt tttaggcggt tttgattttt
     7921 aggatttgag caaatggaca acttatttga tctaaaactt aacatcaatc agatcgccga
     7981 actcacagga ttgcaccgcc agacggtatc tcagcgagtc gctgggttaa atccagcact
     8041 cggtagtaat agcaaattaa aactctatgc tctgcgtgat ttaatcctca ccgggttggc
     8101 ggaaaagatg tcggcagatg tcgatagcct atcacctgct gatcgtcgtg cgtggttcca
     8161 gtcggaaaac gaaagactaa aatttgaaaa agaaattggc gagcttatcc cagcaagcga
     8221 agtggctctt gaaatgtcag ctttagcaaa aacggtagta caagccttag aaaccttgcc
     8281 tgatatttta gagcgagact gcggattgca gcctaaagat ttgatacgag tgcagcaagt
     8341 aactgacgat gtacgagacc aaatggcatt acatatccaa gaagttacca ctgacacgga
     8401 gaatgaataa tgtttgctag tgctaaagaa attcgacgtg atatagcaaa tgccataaaa
     8461 gcacctcggc gaatgagagt gtcagaagca gtagcggaat atatgcgtgt accacttggc
     8521 ggaggcaact cggtcaaatg ggataagcac accgccgcct atatgttaga accgatggac
     8581 tgcctcaata gccgagaata cgatgcggtg atttttgtcg gcccggcacg aacgggtaaa
     8641 accatcggct tgattgacgg ctggataacc tactcaatta tctgcgatcc gtctgatttc
     8701 ctgttggtgc aacttaccca agagaaagct agcgaacata gccgaaaacg gcttgaccgc
     8761 acattccgct gttcgcccga aattgccaag cggctaagcc cacgcaaaaa cgataacaat
     8821 gtccacgaca agtatttcag agcgggcaac ttgctcaaaa tcggctggcc gtctatcaat
     8881 gtgctttctt catcggacta caaatatgtt gctctgactg actacgaccg ctggccggaa
     8941 gatgtggacg gagagggcga cggtttctcg cttgcctcaa agcggacgac cacctttatg
     9001 tcagcaggaa tgactttggt ggagagttcg cccggtaaag atattgttga tctcaaacat
     9061 atcccgaaaa gcacccacga ggctccaccg actaccggta ttttaagcct ctataatcga
     9121 ggtgatcgcc gtcggtttta ctggcaatgc cccgactgct ccgaatactt cgagccgtca
     9181 atggcaaata tggtcggcta ccgtgaagac agtgattttg tcaaagccag cgaaaacgca
     9241 cggttacaat gccctcactg ccaaaccctg attgcccccg aactcaaacg agagctaaat
     9301 atcaagggcg tatggctcaa agaggggcaa aaaattgatg caagcggtca aattagtggc
     9361 gaaagtcgca aatcccgtat tgcctccttt tggcttgaag ggccagcggc agcctaccaa
     9421 acgtgggcgc agctcaccta caaactgctc aatgccgaac acgaatatga aatgactggc
     9481 agtgaggaaa ccctcaaagc ggtaacaaat accgactggg ggttacctta cttaccacga
     9541 tctgcattag aacaacgccg ttctgatgag ttgatggaac gccgagaaga agtagaagag
     9601 aaaactgtac cagctcaatg tcgctttatt gtggcggcgg tggacgtaca gggcggtaaa
     9661 aaccgccgat ttgtggtgca aatggtcggt tacggtgaaa atggcgaacg ctggttgatt
     9721 gaccgctata acatctcaca tactttgccc gacagtgatg gcgtgattga aaaaattgac
     9781 cctcgtattc ccgatgattg gtacatactg atttcggacg tactagaaaa acgctaccca
     9841 cttgcccaca atccaaacca ctttatgccg attttggcga tggcggtgga cagtggcggt
     9901 gaagatgggg taaccgataa cgcctaccaa ttttggcgca aatgccgccg agatggctat
     9961 gcgaaacgag tctatttagt caaaggcgac agcaccaaac ggcaaaagct gattaccaaa
    10021 acttaccccg acaataccgc acgaagtgac cgccactcct ccgcacgggg cgatgtgccg
    10081 ttgtacttgc tccaaaccga ctacctcaaa gatcggatta acaacgccct gttccgtgat
    10141 acggtggggg cgaactacat ccacttcccc gactggctag gtgaatggtt ttttaacgaa
    10201 ttgacctacg aagaacgagg agcggacggc aaatggcgga aaccgggcaa aggcaataac
    10261 gaagccttcg acctgttctg ctatgcccac gccattgcaa ttttacgagg ctacgaacga
    10321 atcaaatggg gcgatgaaaa cgatgtgcca agctgggcaa aactgcccga catcaaccct
    10381 aatatcattc gggaaacgcc aagaacggct gaacaagcgg tcgaaattga agaaaaatcc
    10441 gaaaaaccac aacctaaacc caccaaagca aaaagcagtt ggttaagtgg tggcggtaga
    10501 aaaacaggag gctggctatg agcctttaca ccatagacga gcttaaacaa aaaatccgca
    10561 cgcttgatga aaaaatcgaa accgcccaaa gccaagtgag ctttaatggt cggtcggttt
    10621 catatcaagt ggcggaacta accaaacagc gtgatcgcta tcaaacaatg ctggatgaac
    10681 aacttgccca aagcgggcaa cgcaccaaac agcatcgaat taaatatgcg aggtttattt
    10741 gatgaacttc ctcgaaaaaa ccattgccac cctttccccg aaatgggcgg caaatcgctc
    10801 tcgaaatcgc tatgtgctaa atgcctatga ggcggcacag cctagccgaa cccataaggc
    10861 aagccgagag agcaaagggg caaactctac tgttcgccaa agtgcggtca gcctacggga
    10921 acaagcccga gccttagacc aaaatcacga tattgtgatc ggcattctcg acaaaatgga
    10981 agagcgggtg attggctcaa aaggcattca tattgaaccg cagccgctga cgttggcagg
    11041 tgaagtccac gaagaattag ctgggcaaat ccgaaagctg tgggcggaat ggtcggtgaa
    11101 acctgatgtg acgggattat acactcgccc attgctagaa cgaatgctac ttcggacttg
    11161 gctacgagac ggcgaagtct ttgttcagtt ggtgaaaggc aaggtggcag ggttagaaca
    11221 cggctcacag gttgcttttt cccttgaagc cttagagccg gattttgtgc caatgcaatc
    11281 cgatgaggca aaaaacggct tggtgcaagg cgtgtttctc aatgcgtggc ggaagccgac
    11341 tgcttaccaa gtctatttag acaatccgca agaaagcaac ggaatgtacg gcaaagtgaa
    11401 aaccgtgccg gcagacaata tgttgcacct cgcctttcgc aagcggttac atcaaattcg
    11461 tggtgtaagt atgttgcacg gtgtgattgt gcgattggcg gatctgaaag aatacgaaga
    11521 gagcgaacga gttgccgctc gcattgccgc cgcaatgacg atgtacatta aaaaaggcga
    11581 tgcggcactg tatgatgacg acagtaacag cgatggcgaa cgcctgtttg atattgcccc
    11641 cggtgcggtg attgatgatt taaaaccggg tgaagacatc ggcttaatca attccaaccg
    11701 cccgaatgtg aatttggaaa ctttccgcaa tggacaactg cgagccactg ccgccggtac
    11761 tcgttcgagc tattccagca tcgcccgaga ctacaacggc acttactctg cacagcgaca
    11821 agagctggtt gaaagttttg aaggttatgc ggtgctacaa gatgcgtttg tggcggcaat
    11881 cagccgcccg atttatcggg aatggctcaa aatggcaatc gcctcacaag cgatcaaact
    11941 gccgcctgat attgaccaaa actctctctt taatgcggtt tattcaggtc ctgtaatgcc
    12001 gtggattgac ccaatcaaag aggcgaatgc gtggaaagaa cgaattaaag gcggtttagc
    12061 caccgaaggg caagcaatcc gagccagtgg caataaccca gccgaagtca aacggcaacg
    12121 aattgtcgag atcaaagaaa atcaagccga agggctaaaa tttgataccg atctcaccaa
    12181 ttacggtggg caggataaat ctttctccaa tcaaaaagat gaaattaacg aagatgaata
    12241 acaggagggt gcgtgaaaac gtggttcaac attcaagcca aagcgaatga caccgccgaa
    12301 atctcaattt ttgacgaaat cggcttttgg ggtgtcaccg cccaacaatt tgctaaagac
    12361 ttaaaagccc ttggcaacaa tttaaaacaa atcaatctgc atatccactc gcccggtggt
    12421 gatgtgtttg acggcattgc catttacaac ctgctaaaaa atcaccctgc taacaagacc
    12481 gtctatattg acggtcttgc cgcatcaatg gctagcgtaa ttgcaatggc aggtgatgaa
    12541 atcattatgc ctgaaaacgc tatgatgatg atccacaaac cgtggggcat tcaaggcggt
    12601 gatgcggatg atatgcgtaa atatgcggat ttactggata aggttgaaag tacactgatt
    12661 atggcgtatg tcgccaaaac aggtaaatac gaaaccgatt tggcagaaat gctgaaagaa
    12721 gaaacttggc tcacaggcaa agagtgcgtg gagcaaggtt tcgccgataa gttagccgaa
    12781 ccccttgtgg cgatggcttg tatccaatcc aaaaaattag aggactacac aaaaatgcct
    12841 gacaaaatca aaaatatgct attcgcacca caaggcaacg caggtgcaaa tccaaaacat
    12901 gaacaacctc aaacaccacc acaagcgggt gaaaatccgc aaaatgtggc aaaacctgat
    12961 gcaatggtgg cattagccca acgcaatgca acgattaaag cgacctttgc cgctttcggc
    13021 acacaatttg acggcttgct ggcagaatgt ttagctgatg tgtcaatgac cgctgagcaa
    13081 gccaaagaca agctccttgc caaactgggc gaaaacacca ccccaagcgt accgcaaaac
    13141 cacattcacg caggtaacgg taacatcgtt ggcgacagcg tgaagcagtc gttaatggct
    13201 cgtgctggtc aagataccga caaaaccaac gcaagagata atgcctacaa tgcaatgacc
    13261 ttgcgtgagt tggctcgtgc ctcattggtt gatcgtggcg tgggtattgc gggctacacc
    13321 ccaatgcaga tggtcggtct tgcctttact cattcgagct ccgacttcgg tcaaatcttg
    13381 attgatgtgg ctcataaatc cctactcaaa ggctgggaaa ccgctaccga aaactacgag
    13441 cagtttacca cccgtggcac tcttactgac ttccgaccgg caaaacgagt cggtttaggt
    13501 gagtttggct acctgccgga agtgggcgaa ggtgaagaat acacctacgg cacaatcggt
    13561 gatgaaggtg catctgttgc attggcgact tacggacaac tgtttagcat tacccgtcag
    13621 gcgattatca atgacgatat gcatctttta actaaaatcc ctgagaaaat gggtgcagca
    13681 gccaaagcga ctattaccaa actggtgttt gccttgatta ccggcaatgc gacagcccaa
    13741 gacggtaaaa agctgtttga tgctacacat aaaaacagct taacgggtgc ggcattgaat
    13801 gtggaaaaca ttgacaaggc aatccagtta atgaatggct ttgtaaacag ccgtggtgaa
    13861 ccgctctcta ttgagccaga atttatgctg ttaccaacct cattgcatac caaagcaaaa
    13921 cagattcttg gttcgtcaag cgtggaaggt gcagacatca acagcggtat cattaacccg
    13981 atccgtgata ttgtcagccc gattaaatcg cctcgcttac aaattgcaga tccgaaatca
    14041 tggtacttgc tcaataaaga ggcgattgaa gtctcttatc tggacggggt ggatagccct
    14101 tacatcgagc aacagcacgg ctttacggtg gacggtgttt cgaccaaagt ccgtatcgat
    14161 gctggtgtga atgtgattga ctaccgtggt gtggtgaaag tgaccaacgc ttaatttaaa
    14221 caatttaaaa cctgaccgca cttttaacag tgcggttttt tattatccga aaaaaggaaa
    14281 aagagatatg gctaaaaact atattcaaga cggcaatacc gtgcgtttaa ctgcaaccaa
    14341 agcgattact tccggcgatg tcattgtggc ggaggattta attgcgattg cggtttctga
    14401 tgcggcaaaa aatgatgcag tagttggctt aactaccggt gtttttagcg taaaagctaa
    14461 acaagcagat gacatcaaac aaggtgcagt gttgtactgg tctgaaacag agggcgcaac
    14521 tctcaccgca ggctcaaata aacgacttgg cattgcgtgg aaagactctg gcacatcatc
    14581 taatcaagtt gatgtgaaaa tcaatgtcta gcccgtttga gcaggcaata gctgccgccg
    14641 atcaaactat tcagcaaact atgatgagtg agtggttgat tggcggcaag ccttaccctg
    14701 caacttttga tgaagccccc gctattttta acggtttaca ctcaaccgat gacagagcta
    14761 ttcacggcac ggaacgcaca ctgaccctat ttcgggcgag tggctacaaa ccgagattgg
    14821 acgaccgagt gagcggaaat ggcaagaaat accttgttaa atcctatcac tttgtcgatc
    14881 agttaatcgt attgcaattg gagtaaacaa tgggttctca agtgaccggt ttaaaagaac
    14941 tgcaagcctc cctgaaaaaa atagctaggc aaaccgtacc taaagcagcc gctcaagcaa
    15001 tccgtactgt tgggcggcaa gcaatgaata aggcggttaa atcggtggcg gcagagattg
    15061 gcgtaaatca aaagacaatc aaaggtagag caaaaatgac ggcaaagccc acgccgtcaa
    15121 gattgcaagc cacgattaag gttaatcgat cacatatgcc gatgattcgg gtactagagc
    15181 gcaagtccaa ccgcctgtca cccaccaaag gcagtattag agttgggaaa cacactgtac
    15241 aacgtggttt tcgccaacga ctcgccaatg ggcgaacgca cattatgtac cggcaaggtc
    15301 gtaagcgtta cggcattgat gtagcaaaag tgccgttatc ccaaccactt acacaagcat
    15361 ttgatcgaga attgaaaaac tatcccgaac aagtgcaagc ggaattagct aaacaaattg
    15421 cggggaaatt atgaaaatcc acacaaaaat ccgaaaagaa gtccttgaat tattacagac
    15481 taaattgccg gaaattgata atttttataa cgggctgcca acttttattg atattgagga
    15541 ggaacaactt gcagtatcgg tttatctcga tgatatcagt ctgcaagaaa taactgtttg
    15601 taataatcaa tgggacgcca agctcaatat tacaatccat ctgaaatcta ccgagagtgc
    15661 agaagatgaa ttagacgaat gggcggaaaa aattcgtgaa attatcgatg attatcccga
    15721 atttgagcat ttagagtcag tcgcgctctc tcagtatcaa tatgagcaag accagaatca
    15781 gcgtacgtgg cacatggcga cattaatctt tgatgttgag tattaaacaa ataatctcta
    15841 taggagactt aaaatggcaa aaacaacaaa agtccaaggc acgaaattta gaattagcgt
    15901 tggacgtgag gctcaaaaag caattaccgc aattaactta aatactgcta ctctaacgat
    15961 tgcatcctca ggctataaaa aaggtgatgc aatcgagctt acaggttgtg gtcaattaga
    16021 tggtatctac ccggtgttat cagtaactac cgatcaggta aaattatgcg aagaagtgaa
    16081 ctggacaggt aaagatttac cagctaatta caccaaagcc aaggcggcac tagtgcaata
    16141 ctcggagcaa ttctgcgcgg taaaaaacat tgaaaaatct gatgatactt tgagtaccga
    16201 agatgtaaca acggtttgtt cagagggcac agaaaccgag ccgggtgaaa ttgaatttgg
    16261 ttcgatcaaa ctcagtttct tccacaagcc tactaccgaa atgcaaaccc gattgcgcaa
    16321 actcttttac gacaaatcca cctttgccta caaactggaa ctaccggata accatggcac
    16381 gacctatggc gaaggcttta ttgaggccgg caacggtttc tcgggcgaag ttaagggtaa
    16441 gtatgaaggc tctgtatcta ttaagccaag caaacgcgac tacttattag tttaattgca
    16501 aaaaaaccat aaaattcgac cgtttgtaga aataacaagc ggtcttttta tatcagaatt
    16561 ttacaaaagg aacgaacaat gacactgcgt gagaaactcc ttgctaataa accaaaattg
    16621 caacctatcg aaatcaacgg cgagacctac tacctgcgtg aagctactgt tggtgatatg
    16681 aacaagcaga tttttgaaac ccgaagctgg ctcattcaac aagctgaaca agaaaatgtt
    16741 gaattaccgg cagaagatga tgaaaccttt gatgaggctc tcaaccgttt tggcgaaaaa
    16801 taccgccttg ctcaatcggt tgcctaccgc ttatgtgatg aaaacggtgt attactgttt
    16861 aatcctctcg acattaacga tctgaatgcc attgccgaat tagacagtaa agtgattatc
    16921 gactttaacc aagccgtgtc cgccccaaaa gactcagcga gcgaagaaag ttccaaataa
    16981 ccctttcgct cgcactgggt aaaacacttg aagaaatcga acaaatgccc gaacgccact
    17041 ttgccgaata tcggcttttc taccaagaac agcctttcgg cttatggcga gacgattacc
    17101 gcaccgccca acttgcccac ttaaccgcaa tgttcaaccg tgatccgaaa ggcaaagctc
    17161 caaaattagc ggagtttatg ccgtttttta atcggacaga tgaggtggag gaaatagaag
    17221 atgatggtgt ggcagattat ttggcgaaaa ggtaaagaaa acagcttgaa attccttgct
    17281 cttggatata tatttcctca aagagaagga gggaagtatg tttgaagcac tagaccgttt
    17341 tatgattaat acgattaaaa ttgcaattat tgtatttttg gtcgcttgca tctttattag
    17401 tatgccgaac tggtggatag cttctatctt agtacttttc ccgactgcct atttgcttga
    17461 taaatactgg tggcgaaata ccgaaatgta tcgccgcaaa atggataaat tagttgaaga
    17521 gatgaaaaat aatcgtcaaa tctaaattaa catcaattct agcccgcttt atgcgggctt
    17581 tttatttggg gtgaaaaagt gagtagctca aacagattaa ctattacctt agatgtagaa
    17641 aattctaagt tactagctgg attcgcacaa tctgatacag tagcaaataa gtttgctcgc
    17701 agcttcattg tgaatatgga tcaggctcag caaaaagcac gtcaatttgc tgatagatcg
    17761 acaaaatatc taaaaaatat tgaacaagca gcaaataatc ttaatagctc tgtatcaaaa
    17821 gaacgttttt ggcaaattgc aaatcggttg caattcccga caagtaaagt gattagttat
    17881 gccaatagct acaccgaact cggtaataaa ctcaaattag tcaccgaaag tgaatcccaa
    17941 cacgcccgag caatggcggc ggtgtatgat atttcgctca aaaccgccca atctacacag
    18001 gcaacctctt cggtttatca gacctttgct caaaatgcaa aacagctcgg tatttctcaa
    18061 aacgatgtgg caaaactaac ggaaacggta gccaaatcgg ttgctatttc cggtgcaagt
    18121 tcggcaaccg cttcaaacgc cctagtgcaa ttcagccaat cgttgttaat gggtaagctc
    18181 aaagcccaag aatttaactc attgatgacc caaacaccgt ctgttattca ggcgattgcc
    18241 aaagggttag gcattaccac tgccgaactt aaagcaatgg tggataacgg tgagatgtct
    18301 gctgaaaaaa tgattgaggg gctaaaaaaa gcagagagtt atgtaaacgg acagtacgcc
    18361 aaaacaacca ctacaattag tggagcaatg caaaatcttt caactgcggc tgaaaagtgg
    18421 gttggcgaaa tggatagtac cgttggtgcg tcacagaaag ccgttgccgt tctgaatgcg
    18481 ttaaatgctc attttgatac gttcgctaaa acagcacttg tcgcaggtgg tgcatttgtg
    18541 cttttcaaag gctatatgag tgtacgccca tttatcgagg ctagagcggc gatttatgag
    18601 aatcgaaaag caattattgc ggaaaatcag gctcgccaac aaacgatagc tactcgtcag
    18661 caggaattgg ttgtactctc tcaacaaacc gccttaaaac tggaacaaac attacaaacc
    18721 tacaatcaaa ataaagccga attagaggcg attgcggtta aacagcagaa aatcacaaaa
    18781 gaacgagaag aaattgctgt tgctattcaa gcggcggcaa ctcgtacgga aagattagcg
    18841 ttatctgctg aactgcaagc gctagatcag aaagaaatcg ctctcgggca acagaaaata
    18901 gcggttgaac gccagcaaat tgcaacaaaa caggctctca aagtggcgta tgctgaaaat
    18961 gcagttgctc aaagtgcaat gaatgcaggt atggtcactg caaccacaac aaccagagcg
    19021 atgaatgggg ttttagcggc tgcaagagct gaattgaact tactgaaagc agctgcactg
    19081 tctaacccat taatgactct tgcaatggtg gcaacgactg ccggtactgc cctttgggga
    19141 ttttttgaaa gtcaaaaagc agctcgtgaa gaagccctcc gctacgcgga cagcttagac
    19201 tccgttcgag ccaatatcga aaaaatgact aaggctcaaa ctgatgcaga aatggttaaa
    19261 ttgtctcgtt ctatcaaaga acaggaaaca gcattagcta gtctgaaaca acgccaagct
    19321 gaacttacac gagaaatcga gcaaggagct acggttttag atgatgcctt tggtgccggt
    19381 gtccgtttcg aaaaaagtgc gaaagagtta gcgaaagcac aagaagagtt aaagttagtt
    19441 actgctgatg ttgaaaaagc acaaaataca ttaaatgcca gtttagaagc tcaagaagcc
    19501 ttacaagctc acgttccaat cgctaaccta cgagaggaat ttgtcaaatt atatcctcac
    19561 attgacgaaa gccaaatcaa agttgatggc ttaaatattg ctatcggtaa ttttaccgtc
    19621 aaatcaccgg agatggttat tgccgctaat aacattgcta atgctcttgg tggcgttgcc
    19681 ggagaggcta tgagagcggc tattctagtc gctaatctgt caagtatgaa gctggatgct
    19741 aacggtgctg caattatcga tcctaaacat cttgaaaaaa tcgagcaaat tgaacgtaac
    19801 aatgcgattg cttcggctaa aggcaaagat aaaatcgcat tgcaagtaaa agacgctctg
    19861 attaaatcag gtatgaaaga gggcgatgcc ggatacgagc gactaaaagc agcatatgag
    19921 caacaattta cgttgcaaaa tgcccctaag cgcggcggaa aaccgaagaa aaccaaagcc
    19981 gaacgctctg ccgaaagtta ccaaaaccaa gtagcggaga tgaccaatcg tttatccggc
    20041 ttaaagatag ataaagaaga tctcgtaaaa tatggtggca tttctcaata tcaggaagtg
    20101 cgtaaactta ctgaagatat tgctctgaat gctgaaaaat ataaaaacta cggcactgaa
    20161 ggcgtggcta aactcaaaga gctggctgct aaaattgatt ctgaacaaca gcttttagac
    20221 attgagcaat tccaagtcga tcacgataaa aagctagagg caatgcaatt cgagctggat
    20281 ttactgggaa aaactcgtac tgaaaaagaa ttgttacaat ttaacaatca attagattta
    20341 gaggccgagc ggctgaaaat taatatgacg aaagagaata ttgctctttt agatcagaaa
    20401 attgccaaaa ttaaggaaga atacgagcta catcagaaaa agcaggaaga aactcgtgct
    20461 tctgtaatga tgggaataaa agaaggctgg aacaatattg aaaccgatgt cagcaatgtg
    20521 gcggcaaatg ttgccaatat tactcaaaat acctttgacg gtatggcgga tagcttgact
    20581 aattttgtga tgacgggtaa agcggatttt cgtagttttg ctaattccat attatctgat
    20641 cttagcaaaa tgattatcaa aatgatgtta tttaatgcta tcaaacaagg tgcaactatg
    20701 ttgtttggct accaaggacc gactacaacg gtaaatggta atgctgttta tggattaggt
    20761 acttgggcaa caggtggcta cacaggcaat ggcggcaaat acaccccagc gggtatcgtc
    20821 cacaaagggg aatacgtcat caccaaagag gcaacctctc gtcttggttt ggattatttg
    20881 aattatctta actatggcag acgtggtttt gcctctggcg gtggtgtcgc tgtaccaaga
    20941 gtaccgtcat catcttatca gccgaaatcg gctcaaagta gtatcagcgt acaggtcatc
    21001 aataatggtg agccggtaga tgccaaagtc agccaaaaac agcaaggcga gcaaacgcaa
    21061 attactgttg agttaatgcg taaaattgcc cgtcaggaag cgaacggaat gattcagaac
    21121 aactttagag ccggaggagt atttgcctaa tggaaacatt acaatggtgt gttcgcaccg
    21181 atttagcggt tgaaaatacc cctgaaataa tggaagtcaa attcggcgat ggttataccc
    21241 aacgctcgcc caaaggctta aacaatttat tacgagccta tacaggcaca atcaaagtga
    21301 aaaaggggga acatttagcg gtggaggcat tttttgccaa acatcgtggt gtttcccctt
    21361 tttactttaa agatccgtac acacaacaaa acaaaaaggt agtgtgtagc agttggcccg
    21421 caaaaatggt tggcttaaac cattgggaat ttagtgtaac ctttaaggaa gtaccctaat
    21481 gcctgttagc atatccaatc aaatgaaact cgaccttgcc aaacttgagc aaaatgcaat
    21541 gcttgatttg tacgaggtag atttacgcac attaaaggat aaaagcggta atgctggctc
    21601 agtttaccgc ttttattcgg ggttaaatga actcaaaaca agtattgtct ggcaagggcg
    21661 gacttacgat ccgtacccga ttgaggcaag tggatttgaa cgaaacagta gcggcccaag
    21721 caatcgccct actcttacgc tatcaaatct gtttggctta gttacgggta ttgctaatca
    21781 gtttgatgag tgtatcgggg cgattgttcg ccgtcatcag gtttacgccc aatatttaga
    21841 tgccgtgaat tttgccgggg gtaatgcgaa agctgatcca aatcaggaaa taatcagtca
    21901 ttttgtgatt gagcagctta ccagcctcac ccgagaaacg gcgaccttta cgctggcatt
    21961 accgattgaa actgataatg ccaaaatccc cagcagaatt attatggcgg acacctgcac
    22021 ttggatttac cgctcggcgg aatgtggtta taccggtaag caatattttg atgaaaaaga
    22081 caaggcaaca tcagacccaa aagccgataa atgcagccat tgcctgaacg gttgtgaatt
    22141 gcgaggcaat cagcgtaatt ttggtggatt tgtatcggtg aataaattgg ggtaatgaat
    22201 ggaattggaa caacaaataa tcgactacgc cctccaacac gaaccgcacg aaatgtgcgg
    22261 ctttgttgtt tttgacggca ataaaaatcg atttatccct tgcgaaaacc aagcggaaga
    22321 caaagccaat tactttgaaa tatccgatct tgattacatc aaagcagaag aaaaaggcga
    22381 actggtagcg gtggtacatt cacatcccga gccaaacggc aagcctatat tatctgtgct
    22441 tgatcgcaaa atgcaggtac agaccggttt agattggtgg ctagtgcata atcggcaaat
    22501 ccataaattc cgcaatgtac cacatttaat cgggcgtgag tttaaacacg gcacaatgga
    22561 ttgttacacg ctctaccgtg atgcctatat gcttgccggt tacgagatgg acgaattcga
    22621 tcgacaagat gactggtggc ataccgggca aaatctttat ttggataata tccaagggca
    22681 aggttttgag cgagtgggaa ccccacaaat cggcgatgtg attttaatgc aagtaggtgc
    22741 tgatgtgccg aaccacgctg ccatttacat tggcgaacag atggtaatcc atcatagccc
    22801 gaaccgatta tctaagcgtg atttatacga cggctattgg ttacgtcata cccacagcat
    22861 ttggcggcat aaactggcgg ataagttaga ttttgacggc atattgaatg atattgctgt
    22921 aaataactaa caaaaaatga ccgcttgtaa caaaaacaaa ccccgaagcg tttgcagcac
    22981 ttcggggttt ttcatatcca cttacctaac taagaggaca taaattaaat gaatgataaa
    23041 cgatttacat tcaaatttct aggagttctt atggaagcaa ttaatattac cccaaaagaa
    23101 atcagaaaaa caatgtggac agccgcaata attctctttt tgtttgcgtt catctggaaa
    23161 gcccctgaat ttatcactgc tattcgttgg tggtaactat gattacagtt aaattttacg
    23221 gacacttaag aaaatttggt acagacttta agcttgaggt aaaagacacc gctgaggcaa
    23281 ttcgtgcctt atgtacacag attagaggat tgcgtgaggc attgcgagac ggtgtttata
    23341 aagtccgtat cggtaagcaa tatttagacc catcagccct tgaaaaaggg cttttttatt
    23401 gcttgaagaa agggcaaacc attcatttta cccctgttat caaaggggcg aaaagcgggg
    23461 gtgttttcca agctgtgtta ggtgtagcct taattggagc cgcatttttt ctcgggccgg
    23521 tcggttgggg agttctaggg gcttcttccg caatgatgat gggagcaatg ggggcatcaa
    23581 tgttactagg aggagtttcc caaatgctca ccaaaatgcc aaaagccccc acaatgggta
    23641 atgaaacaga aaaagaaagc tcaacggctt tttcaaattt aaataacttg gtggctcaag
    23701 gcaaacctgt gccgttggct tatggcttaa ttcggaccgg ctcgcttgtg atttcacagg
    23761 gagttgagac gatgactgta gaggtataat tatgggatta tttagtcgta agagaaaaaa
    23821 tcatactcct aaagaagcac cggaaactgg tcgctctaaa cagattgtga atatcgtgga
    23881 gttactctgc gagggtgaaa ttgaaggctt ggtagatggc tttaaatcga tttatcttga
    23941 cggcacgaaa atccaaaacg atgacgacag ctataatttt aataatgtga gcggtcagtt
    24001 aaatgtcggt acgcaagatc aagaggtatt agaaggttac gacagctcac aaaatgaagt
    24061 gagtgtgggt gtagaagtca agaaaaaaaa cggtgcaatc gtccgcaccg ttactgatga
    24121 acgcatcagc cgtttgcgtt taacgttggg cgtgcggtca ctattcca
//
LOCUS       DANLXQ010000018        24066 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.018, whole genome shotgun sequence.
ACCESSION   DANLXQ010000018 DANLXQ010000000
VERSION     DANLXQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24066
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.018"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(86..2488)
                     /gene="mrcB"
                     /locus_tag="PXD94_000581"
     CDS             complement(86..2488)
                     /gene="mrcB"
                     /locus_tag="PXD94_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL6285473.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLEDLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
     gene            complement(2571..3428)
                     /gene="menB"
                     /locus_tag="PXD94_000582"
     CDS             complement(2571..3428)
                     /gene="menB"
                     /locus_tag="PXD94_000582"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL6285474.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            3606..6635
                     /locus_tag="PXD94_000583"
     CDS             3606..6635
                     /locus_tag="PXD94_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HDL6285475.1"
                     /translation="MSLPNYYQDPSVLHLNTTLHHAYFIPFAKGQTPNQLEREQSELF
                     TLLNGDWKFNFYPSPHDLPEDFLNTKFTHQIPVPSNWQTQGFDAHQYTNINYPIPFDP
                     PYVPHQNPCGVYQREITFNPKPNKRYLLNFEGVDSCLFVYVNGEFVGYGQISHSTNEF
                     DITKQLKQGKNSLVVVVLKWCDGSYLEDQDKFRMSGIFRDVYILEREQNYLQDFFIRY
                     QLNADLSKAELKVETQFSTYTEAVHFQLFDPENRLVCEQTSGDFCQEICNLCLWNAED
                     PTLYTLCLSYQNEIIEQKIGFRQIRVENGVMLFNNAPIKFKGVNRHDSDPKTGYSISR
                     EQALVDLRLMKAHNINAIRTAHYPNAPWFSELCDQLGFYLIAESDLESHGSSAVYIES
                     PENSIFLNVPNPNKQEEIRQQAVDNFCYFARDPLFKQAILDRNIANVERDKNRTSVII
                     WSLGNESGFGENFEVAARWVKQRDPSRLTHYEGSIYQHSAHQNDLSNLDLYSEMYPST
                     ERLEEYFSDSANHKPFILCEYSHAMGNSSGDMEDYWQTFQRYSGACGGFVWEWCDHAP
                     LLPNSELSAEQKTEKYGYGGDFGETLHDGNFCMDGLVSQQRVPHSNLLEVKNVNRPVR
                     AELKAGKIWLKNQLDFSDLADYLTVHYCFSENGETTLQGQCLVNCQPNQTACLPLTLP
                     EPNGKLQLLTLDYSLNESTALLEKGHHLGFDQITLHQAMVTPALNPRPQAVIFVQDFA
                     NEWRIQQGDYRYTFDKLKGIFSRIEKNGEAVIEQPLDFNIWRAPTDNDRLIREFWKKA
                     GYDNCYTRAYHTELMQDKNGVNITAECGIVATAQSRILTLKVNYHLAPNGEMTIQVQA
                     TRPAHLPFLPRFGLRFFLNKRNNQVSYFGYGEQESYIDKYHLAKLGAYQTSARENHTD
                     YLKPQENGSHFGTHYVQANGITVTSPQPFSFNLSPYTQEELTTKSHSYDLVESPSTVL
                     CVDYKMSGIGSNSCGPNLKPQYRLNETNFEWLLKLSF"
     gene            complement(6678..8483)
                     /locus_tag="PXD94_000584"
     CDS             complement(6678..8483)
                     /locus_tag="PXD94_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL6285476.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFAPIPIAIVFFFGQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIFATLSAITIAIIALFDLNKQ
                     NNFHIFEKFGIFSSFWAFPVLFLTLEQQTTNDSRYLNRISEMLLNWILSPALIIYTAI
                     IYAYVIFIAAKGQMPDGVVANVALPYLAFGLAIQAVQLLLKNAKWQWFYSHFTYLALL
                     PLTLIWYAIYIRFSHYGLTEARIYLVIGTITLSICYTLLLFKQLAQYRLFSAVSIAII
                     LISVFILDPKTVALKDQTKRLDNYLLKHKLLDVNNKISTKAVLKYQESLNENRDEAEY
                     FYSMLHYVARGMPAAQFKEKYGVNSSYDLMSEIRERNTDPRYFEATNSELVRLTKADM
                     ANAREYITFSSQLYRPNSFSHQKIENKLCQKLLSQGYYFEGNILNIEYQYSETAQDCS
                     SITQDPTEFVIYFKGLGTTIEFNINDILKKIFAKHQLSITQQHKNEVLEKIKGDLLKM
                     DLGNSVLGLKHIELRFEDNIGYIVESISTQYIMLK"
     gene            complement(8636..9589)
                     /gene="corA"
                     /locus_tag="PXD94_000585"
     CDS             complement(8636..9589)
                     /gene="corA"
                     /locus_tag="PXD94_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL6285477.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRKGWL"
     gene            9934..10686
                     /gene="ubiE"
                     /locus_tag="PXD94_000586"
     CDS             9934..10686
                     /gene="ubiE"
                     /locus_tag="PXD94_000586"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL6285478.1"
                     /translation="MQQSAETTHFGFKTVAKEEKQQLVANVFHSVAGKYDLMNDLLSF
                     GIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEVILADINSSMLEV
                     GREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDKDKALRSMFRVLK
                     PGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRYLAESIRMHPKQD
                     ELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            10877..11524
                     /locus_tag="PXD94_000587"
     CDS             10877..11524
                     /locus_tag="PXD94_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL6285479.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
     gene            11685..12662
                     /locus_tag="PXD94_000588"
     CDS             11685..12662
                     /locus_tag="PXD94_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL6285480.1"
                     /translation="MKQIIAQARENAVRAVDFQRVLMYWHIGKRIFEQEQQGQERADY
                     GTYLIKMLAKELEPEFGSGFCSRQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISD
                     SDKREFYLAESCKNNWSSRQLERQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQL
                     IKDPMVLEFLGLKRESAYYEKDLEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDF
                     FVDLVCYNRLLQCFVIFEIKTHKLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCA
                     DKNDAVVKFSLPENQNKIFASQYQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            12736..14373
                     /gene="ubiB"
                     /locus_tag="PXD94_000589"
     CDS             12736..14373
                     /gene="ubiB"
                     /locus_tag="PXD94_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL6285481.1"
                     /translation="MNIKNLNRFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWV
                     KNQHSDKPYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDG
                     KIARQIIEQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
                     GIEPVIKADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIR
                     LRANFENSEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQ
                     VFFTQVFRDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVA
                     FFNRDYRRVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFN
                     VAREFNMEVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAMV
                     RDIKQRLPQYREHFAEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSI
                     SAIVICGAFWKFESLPIWLSVPLLTLFPLVLILSQRK"
     gene            14484..14705
                     /gene="tatA"
                     /locus_tag="PXD94_000590"
     CDS             14484..14705
                     /gene="tatA"
                     /locus_tag="PXD94_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL6285482.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
     gene            14709..15353
                     /gene="tatB"
                     /locus_tag="PXD94_000591"
     CDS             14709..15353
                     /gene="tatB"
                     /locus_tag="PXD94_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL6285483.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            15340..16095
                     /gene="tatC"
                     /locus_tag="PXD94_000592"
     CDS             15340..16095
                     /gene="tatC"
                     /locus_tag="PXD94_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL6285484.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYVVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            16176..17195
                     /gene="hemB"
                     /locus_tag="PXD94_000593"
     CDS             16176..17195
                     /gene="hemB"
                     /locus_tag="PXD94_000593"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL6285485.1"
                     /translation="MQVIQSQYPLRRMRRLRKHDFSRRLVAENQLTVNDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDVKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            17341..18291
                     /locus_tag="PXD94_000594"
     CDS             17341..18291
                     /locus_tag="PXD94_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL6285486.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEELGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            18301..19050
                     /gene="trmB"
                     /locus_tag="PXD94_000595"
     CDS             18301..19050
                     /gene="trmB"
                     /locus_tag="PXD94_000595"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL6285487.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPNFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            19074..19415
                     /locus_tag="PXD94_000596"
     CDS             19074..19415
                     /locus_tag="PXD94_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL6285488.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            19521..20054
                     /locus_tag="PXD94_000597"
     CDS             19521..20054
                     /locus_tag="PXD94_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL6285489.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(20270..21022)
                     /locus_tag="PXD94_000598"
     CDS             complement(20270..21022)
                     /locus_tag="PXD94_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL6285490.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRADK"
     gene            complement(21032..21748)
                     /locus_tag="PXD94_000599"
     CDS             complement(21032..21748)
                     /locus_tag="PXD94_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL6285491.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            complement(21831..22604)
                     /locus_tag="PXD94_000600"
     CDS             complement(21831..22604)
                     /locus_tag="PXD94_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6285492.1"
                     /translation="MLKKLSIAALALTLSVSAFAKESLLERINNKGTITVGTEGTYAP
                     YTYHDASGKLTGYDVEVTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALT
                     SPERRATFDKSESYSWDGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGA
                     DIVPIDGMAQGLLLIQQKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIA
                     NKGNDEALAKISKAIVELREDGTLKKLGEQFFGKDISVQ"
     gene            complement(22784..22909)
                     /gene="ykgO"
                     /locus_tag="PXD94_000601"
     CDS             complement(22784..22909)
                     /gene="ykgO"
                     /locus_tag="PXD94_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL6285493.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
     gene            complement(22918..23190)
                     /locus_tag="PXD94_000602"
     CDS             complement(22918..23190)
                     /locus_tag="PXD94_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL6285494.1"
                     /translation="MKKGIHPENYREVLFYDASVQQGWVIRSCAATNKTMVWEDGKEY
                     PLYTLDTSSASHPVYTGKRREVNTEGRASQFKDRFKGFASTLSNQK"
     gene            23384..24061
                     /gene="rpe"
                     /locus_tag="PXD94_000603"
     CDS             23384..24061
                     /gene="rpe"
                     /locus_tag="PXD94_000603"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL6285495.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGVKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
BASE COUNT         7080 a         4834 c         5171 g         6981 t
ORIGIN      
        1 tagcaaatag ttataatata tactttttat aggtagaatc tatcagcttg tgtatcttga
       61 atgataatgg ctatcaaatt tatttttatt caattggtgc aacttcttca gtagggattg
      121 gttgcttccc ttcagcttct tctgctttag cctcgatatt ttcttttttc agcgaaagtg
      181 catcccaaac acttggtttt tctgtagcca ctacattacc tgcactggta caataacgtt
      241 gccctttatc tcgccaaact ggaatttgtc tgccactatc gcaattccag ctaccatagc
      301 tattaatccc cacccattgc atcgtattaa gtttcggtaa tctatacgcc tgaggcaagg
      361 ctctctcaag atattgctta tacacataca atgcaccact tgaaccggtg aggttggtat
      421 ccccattgtt atctttaccc agccagacgg ttgtgacgtg ttcgccatca attcccacaa
      481 accaagtatc tcgagcgtta ttggttgtac cggttttgcc ggctaatttc agtttggcaa
      541 aatcgttttg taagctacga gccgtacctc gttccacaac ctgttgcatc gcatacattg
      601 tttgaaccgc agcttccggt ggaagcacct gctcgctcac tttgccgcta tctcgctgat
      661 agaacatttc gccattgtgc gaaataatcg cctcaatggt ggttaatggt gttttcagcc
      721 cttcgttggc aattacttga taagagcggg ttacatcgta aggcgagatc gaatatgaac
      781 ctagcaaggt tgatgggtaa ggcggaattt ccgcattatc ccagcccatc tctttttgtt
      841 tggcaatgac tttttttaag ccaactttca taccaatatt caccgttgga atattcagag
      901 agcggactaa ggcgtccatt aacatcaccg agccactaaa actgcggtcg tagttacggg
      961 gtgtccagta ggttgaacca accttaattg aaatttgttt attttggatc ggtgtattta
     1021 aacggaataa ttcaggctgt gccaaggcaa tcgcataaac cgatggtttg actaaagaac
     1081 cgatttggcg ttttgactga atcgcacggt taaagccggc aaattgggtt aagcggtctc
     1141 ctactatcgc cctcacttta cctgtgcggt attccgccac gacaatagcc gattgcaaat
     1201 cgctgacttt tttattttta ctttccagat cttccaaacc ggtaatcact gcttgttcgg
     1261 ctgaacgttg ctgtttacgg tcaagggtcg taaaaatttt agtaccggaa agttgagctg
     1321 acttaatttc gcctaactca tttttcaaat caatggttaa tgcctgcata aaggcaggtt
     1381 gctcacggta aatcgtgcct ttttcacgaa tattaagcgg gcgggagctt aacagagcgt
     1441 aaagttcttg attgatcact tgatgatcca gcaataatcg caacaccaca ttacgtcgct
     1501 ctaccgcacc ttgcggattt cgccacgggt tatacagtga tggacctttt accattccca
     1561 ccaataacgc aatttggtct aagctaatct cttggatcgg acgaccaaaa tagaattggc
     1621 ttgccaatgc aaaaccgtga atttggtagc taccgttctg ccctaaataa atttcgttga
     1681 gataggtttc taaaatgcgg tttttatcgt aacggaagtc taaaatcacc gacattaacg
     1741 cttcatttac cttacgcacc aacgatcttt cattacttaa gaacaagttt ttcactaact
     1801 gttgcgttaa ggtactgcca ccctgcactg ttctgccagc ttggtagtta gtgattaaag
     1861 cacgggcaat accgattggg ctaatcccat cgtgctgata aaagcggcga tcttcggtta
     1921 aaatcagtga ctcaattaac aagcgagggt attgttgtaa gcgtaatgct tgacgttctt
     1981 catcgctatc ggaatgcagt aacgccagta atttcggatc caagcggaac tcctcgatca
     2041 acttaccctg caccaaatct tcaatataag ttaatttatc ttgtttgaaa gttaaacgaa
     2101 gcactcgttg cgattccggt gtttctggaa acgggaaagc tcggcgtagc aacaccagct
     2161 gattttcttc aattttgaaa tcgcctggtg tcgcaacttg agaaacttga cgatagccgt
     2221 tatccagcaa cgcttgtttg gtttgctcaa gcgttaagtt gtcttcaatt ttaatgcttt
     2281 caatacgagc atacacctca gctggtaatt cccatatctg cccatccatt ttgctacgga
     2341 ttttaccgtc taaataaatg ccgtaaaaag ccatactgca agctcctatc accccgattt
     2401 tgaataaggt tgaaaggaaa tagtttcgct ttttcggttt ttgctcaggc tcttgctcgc
     2461 tctgctcgtt taactcaaca tctgacataa aattaactta aattagaaaa ggtaagcggt
     2521 cgatttttgc aaattttttg caaaatcgaa ccgcttgtat ttgccgtcaa ttatggattg
     2581 cggcggaatt tgctgaagtc cggctcacgt ttctcgttga acgcattgcg tccttcttgc
     2641 ccttcttctg tcatatagaa caacattgtt gcattaccag caagctcttg caggccagcc
     2701 tgaccgtcac aatctgcatt caatgctgct tttaagcaac gtaaggcaat cgggctgttt
     2761 tgtagcattt cgcgacacca acgcacagtc tctttttcta aatcagcata aggcacaacg
     2821 gtattgatta agcccatatc taacgcttct tgtgcattat attgacggca taagaaccaa
     2881 atttcacgcg cttttttctg accgactaaa cgtgccatat agcttgcacc ccaaccgccg
     2941 tcaaatgacc ccactttcgg gccggtttga ccgaattttg cattgtcagc ggcaatggtt
     3001 aaatcacata acatatgtaa aacgtgtcca ccaccaatcg catagccggc aaccattgcc
     3061 accaccggtt tcgggcaggt acgaatatca cgttggaaat ccaacacatt taagtgatgc
     3121 acaccgcttt catctttgta gccgccgtaa tcgccacgca ctttttggtc gccgccggca
     3181 cagaacgcat attcgccttc accggttaat acgatgacgc cgattttttc atcaaaacgg
     3241 gcatcagaaa aggcttcaat catctctttt accgtttgcg gacggaaagc attacgcact
     3301 tccggacgat taatggtgat tttagcgata ccgtcttttg atttatggta acgaatatcg
     3361 gtgtaacctt cgctgtgatc aacccactca accggtgcat ataaaaattc ttcgcttgga
     3421 tataacattg gtaaattcct tgaaaaatga cataaaaaat tcttgtgatt ataacggaat
     3481 cgaaggaaaa aggtgaggaa attattttgt gatccgcctc acaatttttc acatagttga
     3541 ttgccctatt ttataagaga attaaagtgt attcgtattt atatcttttt gatcataggg
     3601 cttttatgtc gttaccgaat tactaccaag acccgagtgt gttgcacctt aataccacgc
     3661 tacaccacgc ttattttatt ccttttgcta aagggcaaac gccgaatcag ttggaacggg
     3721 aacaatctga gttatttact ttgctaaatg gcgattggaa gtttaatttc tatccaagtc
     3781 cgcacgatct gccggaggat ttcctcaaca ccaaatttac ccaccaaatc cccgtgccgt
     3841 ctaactggca aactcaaggc tttgatgccc atcaatacac taacatcaac tacccgattc
     3901 cgtttgatcc gccttatgtg ccacaccaaa acccttgcgg ggtgtatcag cgtgaaatca
     3961 cgtttaaccc caagcctaac aaacgctatt tgctaaattt tgagggcgta gattcgtgcc
     4021 tgtttgttta tgtaaacggc gaatttgtcg gctacgggca aatcagccac agcaccaatg
     4081 aatttgacat caccaaacag cttaaacagg gcaaaaacag cctcgtcgtg gtggtgctaa
     4141 aatggtgtga cggcagttat ttggaagatc aagacaaatt ccgaatgtca ggcattttcc
     4201 gtgatgttta tattttggaa cgtgagcaaa actacctgca agatttcttt atccgctacc
     4261 agttaaacgc tgatctttca aaggcagaac tcaaggtgga aacacaattt tccacctaca
     4321 ccgaagctgt gcattttcaa cttttcgacc ctgaaaaccg cttagtctgc gagcaaacaa
     4381 gcggtgattt ttgccaagaa atttgcaatt tgtgtttgtg gaatgcggaa gatccgacac
     4441 tctacacgct atgcttgagc taccaaaacg aaattattga gcaaaaaatc ggctttcgtc
     4501 agattcgtgt ggaaaatggc gtgatgctat tcaacaacgc cccgattaaa tttaaagggg
     4561 taaaccgcca cgattccgac ccgaaaaccg gctacagcat tagccgtgag caagcgttgg
     4621 tggatttacg cttaatgaaa gcccataaca tcaacgctat tcgcaccgcc cattacccga
     4681 acgctccgtg gtttagtgaa ttgtgcgatc agctcggttt ttatctgatt gccgaaagtg
     4741 atcttgaatc tcacggctcc agtgcagtgt atattgaaag tccggaaaac agcattttcc
     4801 tgaatgtgcc aaacccgaac aaacaggaag aaattcgcca acaagcggtc gataatttct
     4861 gttattttgc aagagatccg ctgttcaaac aggcgatttt agatcgcaac attgccaatg
     4921 tggagcgaga caaaaaccgc acttcggtca ttatttggtc acttggtaac gaatccggct
     4981 ttggtgaaaa ttttgaagtc gcagcccgtt gggtaaaaca acgggatcca agccgtttaa
     5041 ctcactacga aggttcgatt taccaacatt cggcacatca aaacgatctt tcaaacctcg
     5101 atttatacag cgagatgtac ccaagcaccg agcggttaga ggaatatttt tccgactctg
     5161 ccaaccacaa accttttatt ttgtgtgaat attctcacgc gatgggcaat tcgagtggtg
     5221 atatggaaga ttattggcaa accttccaac gctacagcgg tgcgtgcggt ggctttgtgt
     5281 gggaatggtg cgaccatgct cctctgctcc ccaactctga gctaagtgcc gaacagaaga
     5341 ccgaaaaata cggctatggc ggtgatttcg gcgaaacctt gcacgatggc aatttctgta
     5401 tggacggctt ggtttcccaa cagcgtgtgc cacactctaa tttgttagaa gtgaaaaatg
     5461 tgaatcgccc ggtgcgagct gagttaaaag cgggcaaaat ttggctgaaa aatcagttgg
     5521 atttcagcga cttagctgat tatctgaccg tgcattactg tttcagcgaa aacggcgaaa
     5581 ccaccctgca gggacaatgt ttggtaaatt gtcagccaaa tcagaccgct tgtttgccgc
     5641 taacgctgcc cgaaccaaac ggcaaattgc aacttttaac ccttgattat tctttaaatg
     5701 aaagcacggc tctgttggaa aaagggcatc acttaggctt tgaccaaatc acgctgcatc
     5761 aagcgatggt cactccagca cttaatccac gcccgcaagc ggtcattttt gtgcaagatt
     5821 ttgcaaatga atggcggatt caacagggcg actatcgcta cactttcgat aaactcaaag
     5881 gcattttcag ccggattgag aaaaacggcg aggcagtaat tgagcaaccg ttggatttca
     5941 atatttggcg agccccaacc gataacgacc gcttaattcg tgagttttgg aaaaaagccg
     6001 gctacgataa ctgttacacc agggcgtatc ataccgaact gatgcaagat aaaaacggcg
     6061 taaacattac tgccgaatgt ggcattgtgg caaccgctca aagtcggatt ttaaccctga
     6121 aagtgaatta tcatcttgcc ccgaatggcg aaatgacgat tcaggttcag gcaacccgcc
     6181 ccgctcattt gccgttttta cctcgctttg gcttgcggtt tttcttgaat aaacggaata
     6241 accaagtgag ctattttggc tatggcgagc aggagagtta tatcgacaaa taccatttgg
     6301 cgaaactcgg agcttatcaa accagcgccc gagaaaatca taccgattat ctcaaaccgc
     6361 aggaaaacgg cagccatttc ggtacgcatt atgtgcaagc aaatgggata actgttactt
     6421 ctccacagcc gtttagtttt aatctttcgc cttacacaca ggaagagcta accactaaaa
     6481 gccacagtta tgatttggta gaaagccctt ccaccgtgct gtgtgtcgat tacaaaatga
     6541 gcggcattgg ctcaaactct tgcggcccga atttgaaacc gcaatatcgg ttaaatgaga
     6601 ctaattttga gtggctgctt aaattgagtt tttaacgaaa aatgctcgtt caaatttaat
     6661 tgaacgagca tctcctttta tttcagcata atatattgtg tactaatact ttctactata
     6721 tagccgatat tgtcttcaaa tcttaactca atatgcttaa gcccaagtac tgaatttcct
     6781 aaatccattt tgagtaaatc gcctttaatc ttttctaaaa cctcattttt gtgttgctgg
     6841 gtaatagata actgatgttt agcaaagatt tttttcaaaa tatcgttaat gttaaattca
     6901 atagtagtcc caagtccttt aaagtagata acaaattcag taggatcttg tgttattgaa
     6961 gagcagtctt gagccgtttc tgagtattga tactcaatat ttaatatatt tccctcaaaa
     7021 tagtaacctt gagaaaggag tttttggcat agcttatttt ctattttttg atgggaaaat
     7081 gaattaggcc gatataattg gctactaaat gtaatatatt cacgagcatt tgccatatct
     7141 gctttagtta agcgaaccag ctcagaatta gtagcttcaa aataacgtgg gtcagtattt
     7201 ctttctctta tttcactcat taaatcgtag cttgaattta cgccatattt ctctttaaat
     7261 tgtgcagcag gcattcctct ggcaacatag tggagcattg aataaaaata ttcggcttca
     7321 tctcggtttt catttaatga ttcttggtat ttaagtacag cttttgtgct gattttgtta
     7381 tttacatcta ataatttgtg ttttagcaaa tagttgtcca accgcttggt ttgatcttta
     7441 agcgcaactg ttttcggatc taaaataaac actgaaatta aaataattgc tatcgacact
     7501 gcagaaaaga gacgatattg cgccaactgt ttaaatagta ataaggtata gcaaatagat
     7561 aaggttattg tgccaatcac taaataaata cgggcttcag ttaagccata atggctgaaa
     7621 cgaatataga tggcatacca aattaatgtt aacggcaaaa gtgctaaata ggtaaagtga
     7681 gaataaaacc actgccattt tgcatttttt agtaataatt gcaccgcttg tatagctagg
     7741 ccgaaagcta aatagggaag cgctacgttt gcaacaaccc catctggcat ttgcccctta
     7801 gccgcaataa aaattacata agcataaata attgcagtat agataattaa tgccggagac
     7861 aaaatccagt ttagtaacat ttcactaatg cggtttagat atctagaatc attggtagtc
     7921 tgttgttcaa gagttaaaaa taaaacaggg aatgcccaaa aactagaaaa aatgccaaat
     7981 ttttcaaaaa tatgaaagtt gttttgttta tttaaatcaa ataaagcaat aatggctatg
     8041 gtaatagcgc ttaatgttgc aaaaataatg ccagataaaa ctgtagcaaa gacaatgtta
     8101 actaatttat tagttgttcc ggctacaaaa taatggttgt ttttgtacca atattgactg
     8161 atcaaacaaa ggaatacaca aaatatggta gcccaataac gagaatcttc caataatgtt
     8221 aaattaattt gaccaaagaa aaagacgatt gctatcggaa tcggagcgaa tagccaataa
     8281 gctttggtat cgcgtatcag gtaaagcaaa ataaagcaaa taggaaaaag agcaattaga
     8341 ttttgaaagc gtttgattaa ttcgtagtcg ctcacattat tgataagtaa aatcgtgacg
     8401 taagtaacga ataaaatttc cagtggatgc ttaatgatgg tgctttttat cattaaggta
     8461 aagcgttgta gcttacttag cataatagat ctcctgccta tgaaaagaat atctcacagt
     8521 atatatgtga ataaataata ggtaaacagt tttaatcttt atttgttttt agaggtgaac
     8581 atattcatta aaataagaaa gcctaagtta ttcaactata ggctttaaaa ataaattata
     8641 accaaccttt acgtttaaaa taaagataag gggcggaggc ggcaacaatc attaaaccaa
     8701 tcgccatagg gtagccatat tggaaatgaa gttccggcat aaattcaaag ttcataccgt
     8761 aagtagaagc aaccagcgta gcaggtagga acatcactga caccaccgag aagaatttca
     8821 taattcggtt ctgctcaata ttgatataac ccattgccgc ttgcattaaa aaattcactt
     8881 tttggaacaa ggattcattg tgcggttgca gagattcaat atctcgcata atgtcacggg
     8941 cttgttctag ctgattattc ggcaaacggg ttttacgcag taagaaactt aacgcccgtt
     9001 gcgtatccat caaacataaa cgcacttttg aacttgcatc ttcaaattca gttaagcgag
     9061 aaagggcatc attgaaattc tcattttctt gctgaccgtt tagaataacc cgacttaatt
     9121 tttctaagtc agcatagact tcttcaatta cgtctgcaag ctgctcgatt ttggtctcaa
     9181 ataaatcgag taagagttca taggcgttgc tgtcaatcaa tttctcacgg cgggaacgca
     9241 ttcgatacaa gcgaaatgcg ggtaaatccc gatcacgcaa ggtaaacaaa cgcccgtcac
     9301 gaatagtgaa ggcgacagtg gcaatatcgg cgtaatcgtc atcatctaag cagtaaaaga
     9361 acgagtgcag gtgcaaaccg tcttcatctt caaagaaacg ggcagacgcc tctaagtctt
     9421 ccaattcacg ttcttcagcg agtgtttggt ctaagcggtt ttctaatacg gttcgttcag
     9481 cttctgatgg atcgatgaga tcaatccaaa tcgcatcatt taataaagtt gggttagttt
     9541 catcaacgct gattaaacat gcgttatcta gtccaaaagc acggatcatg tttagctcca
     9601 aatagttagg aggcttggaa caaaaaggaa caaagatact aggcgagcat taccgacctt
     9661 gtcggtaaca ttgaggttag acaagatagg ttaaacgggt aagtatcgac tatgactgtc
     9721 caaaatgtgt aatcctcaag aaaaaatagt gtgcgaatgt tacagaattt ggcttttcta
     9781 gtcaagaaag cggttattta cttaagaaaa tttgcaaaaa gttaaagaaa aacgaccgct
     9841 tttttgctat ggttacgcct aaattttgca actagagaca agaaaatgag cgatgtagaa
     9901 caattagcac ctaaagatga agtgcaacaa gaagtgcaac aatcggcaga aaccacccac
     9961 tttgggttta aaaccgtagc gaaagaagaa aaacagcaac tggtcgcaaa tgtgtttcac
    10021 tcggtggcag gcaaatatga cctaatgaac gatttgcttt ctttcggcat tcatcgagtg
    10081 tggaaacgct ttaccattga ttgcagtggc gtgcgtaaag ggcaaaaagt gctggatctt
    10141 gccggcggca cgggtgattt taccgcaaaa ttctctcgca ttgttggtga aagtggtgag
    10201 gtgattttag ccgatattaa cagctcaatg ttggaagtcg ggcgggaaaa attacgcaat
    10261 ttaggcgtgg ttggaaatgt gagctacgtt caagcgaatg ccgaatgctt gccttttgct
    10321 gataacactt ttgattgtat tgtgattagt tttggcttgc gtaatgttac cgacaaagac
    10381 aaagccctac gttcaatgtt ccgcgtgcta aaaccgggcg gtcgcttact tgtgttggaa
    10441 ttctcaaaac cgattatcga cccaatcagc cagctttata atttctactc attcaatatt
    10501 ttgccgaaag tgggcgaagt ggtggtaaat gacgccgaaa gttaccgcta tttagctgaa
    10561 tcgatccgaa tgcacccgaa acaagacgag ctaaaagcaa tgatggaaca aactggcttt
    10621 gaaagcgtga attactacaa cttaagtgcc ggcattgtgg cattacacag aggttataaa
    10681 ttttagtttt ctcttaaatg actctctccc tttgtgggag agagaccgct tatgagcgtt
    10741 tagcgaaata agcagagaga gggggcaagc ggtcatattt attcaatatt ttgcaaatac
    10801 ccctctccct gattttgctt cttaatgaag aacaatctat ccttctccca aaagtgaaga
    10861 gggtaagtag gtacgaatgt taaataatgt aaaacaacaa ttaatgctcc cgcaatttac
    10921 ctttggtctg attgaaaccg cttttaatgc gttacttaaa cgctcgcctc atattgagcc
    10981 gaatttgcgt aaattgtccg gaaaagtgct aaaaatcgcc ctaacttcgc ctaaaatcga
    11041 cttttttgtg ttgtttagcg aaagccgaac cgattggctt ggcaactacg agggggaagc
    11101 agattgtacg gtgcaactgg catttgacac cctgccgaaa ctagcggata agcaaaaatt
    11161 aaccgagctg attaataata aatcgttggt gctgacgggc gatattcaag tgttgcaaca
    11221 tttcactgca ttacttgaac agctcgaaaa agaccctgcc gaactgcttt ccccttttgt
    11281 tggcgatgta gtggctcaaa ctagcaccga tcttgctaaa aaattgctag gtaaagtaaa
    11341 agctcaattt gagcaaaata atcaacattt ggttgagaat ttaatccaag aaagaccggt
    11401 gttggtgcat cgtttacagg cggtgcattt ctatgaccaa gtcgaagaac ttgcccaaca
    11461 agcgctcaaa ttagagcaaa aatttactca ctacgttcgc tcttcgagcc atcagcaaag
    11521 ctgatgttca aacgcaagcg tttgtgcaaa attaaattta acaaaataaa attgtgatat
    11581 gactcgaaaa gttgagtgaa agaaattgcc tccggataga ttaagcataa acttaatcca
    11641 aaacggaggc ttttatgtta atagaaacaa cgatgatcta agatattaaa caaattattg
    11701 ctcaagctcg tgaaaatgcg gttcgtgcgg tagatttcca acgtgtgctg atgtattggc
    11761 acatcggaaa acggattttt gaacaggagc aacaagggca agagcgtgct gattatggaa
    11821 catacctgat taaaatgctc gcaaaagagc ttgagccaga atttggaagt ggtttttgta
    11881 gccgtcaatt agagcgttat cgccagtttt atcgtgcgtt tccaattgcg accgcactgc
    11941 ggtcgcaatt gaattggaca caatatcgtc gtctaattca aatctccgat tctgacaaaa
    12001 gggaatttta tttggctgaa agttgtaaga ataattggag ttcacgccag ctggaacggc
    12061 aaattaatag tagtctatac gagcgattat tgctcagtaa tgataaggaa agtgtgcttg
    12121 ctgttgccag aaatgagcag cagccggttg atccgaaaca gctcattaaa gacccgatgg
    12181 tattggaatt tctcggacta aaacgggaaa gtgcttatta cgaaaaggat ttagagcaag
    12241 ccatcattac gcatttgcag gattttctac tagaattggg taacggtttc tcgtttatcg
    12301 cacgccaaaa gcgtttgcac ctagacggtg atgatttttt tgtcgatttg gtttgttaca
    12361 accgtttact tcaatgtttt gtgatttttg aaattaaaac ccacaagctc acccatcaag
    12421 atttggggca attacagatg tacgtcaatt attttgaccg agttgaaaaa ctgccgcacg
    12481 aaaatgccac tattggtgtc ttgctttgtg ccgataaaaa tgatgcggtg gtaaaatttt
    12541 cgctaccgga aaatcagaat aaaatttttg ccagccaata ccaactttac ctcccctctg
    12601 aacaaacact tattgcggaa gtacagaaag aaattgagaa ttttgagcag aaatacccat
    12661 agtagcccaa caagcggtta aatttttaca gaattttgct aaaatcttcc taattaccat
    12721 taaatatata tttcaatgaa catcaaaaat ctcaaccgct tttaccatat tatccgcact
    12781 tttcttcgct atggtattga tgaagccatt ccgaatctgc caattacccg caaaataaag
    12841 ctagggcgta aggctttgtt ttgggtgaag aatcaacatt cagacaaacc ttacggtgta
    12901 cgtttacgtt tggctttgca agagcttgga ccggtttgga tcaagctcgg gcaaatgtta
    12961 gctaccagac gtgatctgtt tgcaccggag cttgccgacc agctcgcctt gctacaagat
    13021 aatgttgaac cgtttgacgg caaaatcgct cgtcaaatta tcgagcaggc tttgggcgga
    13081 aagcttgaaa cttggtttga aaactttgat gaaactgcct tagcttctgc ttcgattgca
    13141 caggttcaca cggcaaaatt taaccaaaat caaccgcttg caggcaaaga tgtggtgtta
    13201 aaagtgattc gcccgggcat tgagccggtg attaaggcgg atttagagtt gatgtatgaa
    13261 attgctcact ttatcccgaa attatcgaat gacggcaaac ggctacgggc ggttgaagtg
    13321 gtgcgagagt acgaaaaaac tattttagac gagctggatt tacgcaaaga aatgcacaat
    13381 gccattcggc ttcgggcgaa ttttgaaaat agcgaaatgc tgtatgtgcc tgaaatgtat
    13441 caagattttt gccataaaaa cttgattgcg atggagcgga tttatggcat tccagtgtct
    13501 aacattgccg agttggaagc gaacagcacc gatatgaaat tgctggcaga gcgtggtgtg
    13561 caggtatttt ttacccaagt attccgtgac agttttttcc acgccgatat gcacgccggc
    13621 aatatttttg tgaacagaga acacccggaa aatccgcaat atatcggcat tgactgcggg
    13681 attgtcggca cgttgaacca caatgacaaa cgctatttag ccgaaagttt tgtcgcattt
    13741 ttcaaccgag attaccgccg tgttgcctta atgcacgtgg aatcgggctg gacgccgccg
    13801 gataccgata ttgataaatt tgagcaagcc tttcgtgagg tatgtgagcc gatttttgct
    13861 aagccgttat cggaaatttc attcggacac gttttgctca acctgtttaa tgtggcaaga
    13921 gaatttaata tggaagtgca gccgcagttg gtgttgttgc aaaaaacact tctttatatt
    13981 gaaggtttgg gcaggcaggt ttatccacag ttggatttat ggcaaaccgc aaaaccgttt
    14041 ttacaaaact ggctgaatga gcaggtaggt gtgaaagcga tggttcgaga cattaagcaa
    14101 cgtttaccgc aatatcggga gcattttgcg gagtttccgg aggcattgtt taaagcctta
    14161 caagagcaaa agcaaattaa tttccgttta gcggaaatta acgaaagttt aaaagcacag
    14221 aattcaaacg caaatagccg ttttcttgtg ctgtcaattt cagcgattgt gatctgtggc
    14281 gcattttgga aatttgaatc tttgccgata tggctaagtg tgccgttatt aactttattt
    14341 ccactggtat tgattttgag tcaacgcaaa taagcggttg tttttgacaa attttttgca
    14401 aaattacttt tttatatgtc aaagtaacat ataataaaca gcgttttatt ttatctattc
    14461 cctattaaat gaaaggagtt tttatgggcg gtattagtat ttggcagctt ctgattgtgg
    14521 ttgcaatcat cgtattactt ttcggtacga agaaattacg tactttaggt tcggatttgg
    14581 gcgaatctgt aaaaggcttc aaaaaagcaa tggcagatga gaaaaaagat gctgaatttg
    14641 aaaaagtgga agcaaaagat gcaatttcag cagagcagaa agcaaaagag aaagagcagg
    14701 cttaatccgt gtttgatata ggtttttctg aattagtact gattatgatt gtgggcttgg
    14761 tggtgctagg cccgaaacgt atgccgattg cgattcgtac cgtaatgggc tgggtgagta
    14821 caattcgagg tttagccgca aacgtacaaa atgaactggc tcaagagctg aaactgcaag
    14881 aattaaaaga gagcattaaa aaggcagaag agttgaattt aagctccctt tcgccagaat
    14941 taaataaaac ggtggaagat ctgaaaatgt cagccaccca gttgcaagat tcgcttaatt
    15001 ctgcaaaaaa tgagttggaa aaaacggctg cggaaagcag cacaattcct aaattaaccg
    15061 atgagcaagt cgcagaaatt cagcaaaaaa tcgagcaaga aagccaggtg ttagaggcgc
    15121 aagaattgca aaaaaatgcg caaaataaac cgcttgaagt ggatagttta tctgagcaac
    15181 ttaatgttac gcggacagaa aagccacttt cggctgatga gctggctgaa caagcagaaa
    15241 tggacgaatc gcttttaacg ttggaatctt actatccgcc agatgatgat ttagctacgc
    15301 ctcaatcatc agaatcagcg cagaaatcgg aggcaaataa tgtcgcaagt tgatgagtct
    15361 caacctctga ttacacattt aattgagctg agaaaccgat tactgcgtag ttttatctgt
    15421 gtgctcttgg tgttttgcgg attggtttac tgggccaatg atatttacac cttattggca
    15481 acacctttaa cggacaattt accagacggt gcaacaatga ttgcaaccaa tgtagccact
    15541 ccgtttttta ccccaattaa attaaccatt gtggtttcgg tgtttttatc agttcctttt
    15601 attttgtacc aaatttgggc gtttgtcgcc cctgctttat acaagcagga aaagcgtttg
    15661 gtttacccat tattggtatc tagcacattg ctgttttatt gtggtgtcgc ttttgcctat
    15721 tatgttgtat ttccgcttgt atttggcttt ttaacctcta ccgctccgga aggggtaaca
    15781 atggcaacgg atattagtag ctatcttgat ttcgttttaa ccatttttat ggcattcggg
    15841 atctgctttg aagtgccggt tgcgattatc ttgttatgtt gggcaggcgt aaccacaccc
    15901 gaagatttaa aggcaaaacg cccttatatt attgtggcag catttgtaat tggtatgttg
    15961 ctcaccccac cagatatttt ctcgcaaaca ttattggcaa ttccaatgtg tttattgttt
    16021 gaagttggtg tgctatttgc tcggttttat cgccccaaag atgatgatga accatcagag
    16081 caggttgcac agtaatattt tgaaaaggca tagcacctgt ttgctatgcc tttcttattt
    16141 caagaatagg tattttaaga attaggaaat taaaaatgca agtaattcaa tcccaatatc
    16201 ctttacgccg tatgcgtcgt ttacgcaagc acgatttcag ccgccgctta gtggcagaaa
    16261 atcaacttac cgttaatgat ttgatttacc ctgtttttgt catcgaaggg gaaaatcagc
    16321 gagtacaagt gccttctatg ccgggtgtag agcgtttaac tatcgaccag cttttagttg
    16381 aagccgcaga actggttaaa tatggcgtgc ctgcggtggc tcttttccct gtggtgggtg
    16441 atgtgaaaaa atcactgatg gcagaagagg cctataaccc gcaagggttg gcacagcgtg
    16501 ccgtgcgagc tttaaaagct caatttccgg aattaggcgt gattactgat gtggcgttag
    16561 acccgtttac cacgcacgga caagacggca ttattgatga aacaggctat gtactgaatg
    16621 atgtgactac tgaagtattg gtaaaacagg cgttatcgca cgcagaagcg ggggcggata
    16681 ttgttgcccc gagcgatatg atggacggtc gaatcggcaa aattcgtgaa gcccttgagc
    16741 aaaacggttt tatcaatacg caaattatgg cgtattcggc taaatatgcg tctaattatt
    16801 acggaccgtt ccgtgatgca gtaggttctg ccggcaactt aaaaggcggt aataaataca
    16861 cttaccaagt cgatccggct aacggcaacg aaggcttgca cgaagtggcg atggatattc
    16921 aagaaggggc agatatggtg atggtgaaac cgggtatgcc ttatttggat atggtgtggc
    16981 gtgtgaaaga gaattttggc gtgccaacct tcgcctacca agtttcgggc gaatacgcga
    17041 tgcatatggc ggcaatccaa aacggctggc tgaaagagcg tgagtgcatt atggagggat
    17101 tactctgctt taaacgggca ggagcagacg gtattctaac ttatttcgcc aagcgagtag
    17161 cgaagtggct atacgaagag gctcaagcga aataaatact agcaagcggt ggttctagtg
    17221 caaaatcgtt cgtgaatgca acttttttgc taaaaatgac cgcttgtaaa gcctatatat
    17281 ttgttggttt tcaagctata ataacggatt atcatttaat tgattgagaa taataaaaat
    17341 atgaatagta gaattttagc aacgggaagc tatttaccga caaaggtgcg taccaatgct
    17401 gatttagaaa aaatggtcga tacctctgat gaatggatcg taacccgttc cggcattcgt
    17461 gaacgccgta ttgccgagcc tgacgaaaca gtggcaacaa tgggggcaaa agccgctcaa
    17521 caagcactgg aaatggcagg aattgaggca aatgacattg atttaattgt ggtgggaaca
    17581 actaccaact cccacgctta cccaagtgca gcttgccaaa ttcaaggctt gcttcagatt
    17641 gaagacgcga ttgcttttga tgtggcagcg gcttgtacag gatttgtgta tgctttaact
    17701 gttgccgatc aattcgtgcg taacgggcaa gtgaaaaagg cattggttat tggttcagac
    17761 atcaattcca gaatgttaga cgaaaccgac cgcagcacag tagtattatt tggtgacgga
    17821 gctggggcgg tgattttaga ggcgagtgaa gagctgggta ttatttcaac ccacttgcac
    17881 gcctcaccgg atttagaaaa taacttaacc ttaagaagcc aaaagcgtgg cgaagagcgt
    17941 tccggcttta ttgaaatgca gggcaatgcc acctttaaaa ttgcagtaaa ccaactttca
    18001 agcgtggtag aagaaacctt agaagcgaat aatctacaaa aatccgattt agattggctg
    18061 attccgcacc aagcgaattt acgcattatc accgcaacgg cgaaaaaatt agagatggaa
    18121 atggatcaag tggtggttac ccttgataaa tatgccaata ccagtggagc aacagtgccg
    18181 gtggcattag atgaagcggt gcgtgacggg cgaattcaac gtgggcaatt actactcctt
    18241 gaagcattcg gcggtggctg gacttggggt tcggtattag tgaagtttta aggcaagaaa
    18301 atgtcagaaa aacagacttt tgcagataaa aaacgtaaaa ccgttgagca agccgagttt
    18361 accgaagatg ggcgttattt acgaaaagtg cggagttttg tattgcgtac cggtcgtttg
    18421 agcgattatc aacgtgatat gatgaacaac aactgggcaa atttaggctt agattaccag
    18481 ccaatgccct ttaattttga gcagattttc ggcaataaca acccggtagt gttagaaatc
    18541 ggcttcggaa tgggaaaatc gctggtggaa atggcagagc aaaacccgaa tcgcaactat
    18601 atcggcattg aggtgcacac gcccggggtg ggggcttgta tcgcttatgc gttagaaaaa
    18661 ggcgtgaaga atttaagagt aatttgccac gatgccaccg aaattttgcg agacagcatt
    18721 gctgacggta cattaggtgg cttacagctt tacttcccag acccttggca aaaagcaaaa
    18781 caccacaaac gccgtattgt gcagcctgaa tttatcagcc gtgtgatcag aaaattaagc
    18841 cctaacggct ttattcattt tgccaccgac tgggaaaatt acgcagaaca tatgcttgaa
    18901 gtattacgcc aatttgagag cgagttgcaa aatacatcag caactaatga ttttattcca
    18961 cgtccgaatt tccgcccgct aaccaaattt gaagagcgag ggcatcgttt aggacacggg
    19021 gtgtgggatt tatattttac aaagaaatga tttattcata gaggagagaa aaaatggcta
    19081 ttcaacgtaa cgcacgccaa cgtaaaaaaa tgcacttagg tgaataccaa gaattaggtt
    19141 tcttaattaa attcaccttt gctgaaggca caggcattga ccaaattgat gaaaccgtag
    19201 atcgttttat cgatgaagtg attcgaccaa acggtttagc ccttgaagcg aacggctact
    19261 taaactggga aggtttagtc tgcttagaaa aattaggcaa atgtgatgaa tctcaccgcc
    19321 aattagtgaa aacttggtta gaaactaacg gcttaaaaga ggttcaagtg agcgaattat
    19381 ttgatatttg gtgggactac ccagcacaag cttaatttgc ctagttaatt taataagata
    19441 caagcggtca gattttactg aaaatttgca aattttttct aaaaaacgac cgcttgtttt
    19501 gtatagaaag agaaaaagag atggaatttt tagctcctgt tatttcgctt attgttggct
    19561 ttttaagttt tgtgctggtg ctaagaactt ggctgcaatt ttgtcgggtt gatcctcgtt
    19621 taccgatttc acaaacctta cttaaaatta ccgaaccgat ggtttcaccg gttggcaagc
    19681 tgattccaac cgttaaaaat atttgctttc cggcactatt aattgccgcc gctttggtaa
    19741 cgttacagtt tattctgttt ggcgtagatt tacccagagc agtattaatc ggcttattaa
    19801 gtgtggtcaa aaccttcgga caaattctat ttttcaccac cttgattcgt gcattaatga
    19861 gctgggtcac tcaaggcaac cacccattag actacacggt agcacaaatt accgaaccgg
    19921 tgttaggttt tatccgcaag ctcctgccaa gaacgggaat gttagatttc tcggtaatgg
    19981 tgctaggttt cggcttattg ctactaaata gcttgttcta ccaaattttt ggtggactat
    20041 gggctgcggc ttaaatcgga tttctaaaat ctaaaaataa gctccttaat ctaaaagatt
    20101 aaggagcctt gtcttattta tcctttatgg ggaaccttgt tagctgattt tactgtacga
    20161 tggcgcgagc gtccacgctc gtgccaaaac agtataacaa tttatacata attaaccgct
    20221 tgtaccattt attagatagc actagcgtgg acgctagcgc tatcgtttct tatttatccg
    20281 ctctgatttg atttaaaaaa cgtttagttc gttcgtgttg tgggtggtta aacagttgct
    20341 gtggcgagcc ttgttcaacg atttcaccgc cgtccattac caccactaca tcggcaacat
    20401 caagggcaaa tttgatttcg tgagtaacca cgaccattgt ccagccttca tttgccagtt
    20461 ctttcatcgt atttagcacg tcttgcacta actcaggatc aagtgccgaa gttggttcat
    20521 caaataacat cagctccggc tgaattgcta atgctcgtgc aatgccaacc cgttgctgtt
    20581 gcccgcctga cagttgaaac gggtagagtt ctgctttatc cgccagtccg actttttcta
    20641 acagggcttt tgcatttttt tgagcaattt cgaccgcttg ttgttgcaca aataccggtc
    20701 cttccatcac attttccaat gcggttttat gcgggaacag gttgtagttt tggaacacca
    20761 taccggattt gcggcgtagc gctaaaatct cttttttgct ctgttttacg gcaaaatcaa
    20821 ttttcagcgg agcattacca ctaaattcca atgtgccttg ctcgggcatt tccagtgcat
    20881 ttaagcaacg aagaaaggtg gttttgcctg aaccggaagg gcctaaaatt actaccactt
    20941 gccctttttg aatatctaaa tcaatgccgc gtagaatagc gttgttgcca aaggttttat
    21001 gaatattgcg aattttaagc acaattcctc cttatttcgc cacaaatcgg ttaaagcgga
    21061 cttccaatct cgcctgaatt aaaaatagca cccagcagaa gcaccaataa atcaaaccgg
    21121 cttcaatata aacaggtagg aagtcataag acatatttgc tacctgttga gcaacccgga
    21181 acatttcggt tacggtaaca acggaggcaa gagaggtatc tttaaacaga ccgataaagg
    21241 tgttgctaag tggcggaaca gcaactcgaa acgcttgcgg ggcaatgata cggcgaaaag
    21301 tttgcatata agtcataccg atagtataac cggcttccca ttgtcctttt ggcacggagg
    21361 agatcgcagc acggatcgtt tccgagccat aagccccgat attgagtgaa aacccgataa
    21421 ttgccgccgg aatcggatcg ataaaaatgc cgattgcagg caaaccataa aacacaatac
    21481 agatctgaac caacatcggc gtgccgcgaa taatcgagat ataacctttt accagccaca
    21541 gtagcaagcg gtgaaaaaag cctgttgcag ggctaattcg cactaatgca actgcaacgg
    21601 caattaacat accgataata aatgaggcga ttgccagcgg aatagagact aaaaatgccg
    21661 cttgtaccat cggccaaaaa ccgcttagca ctaaatcgac ccgagcttgg gtcataaagg
    21721 ggattgagag aagtagatcg tttaacatct gaaaaaccta aaatctcccg ttttgaggga
    21781 gatttaaaaa taaaaggggc gaattcgccc caagcctaat ccaatatgga ttattgtaca
    21841 ctaatatctt taccgaagaa ctgctcgccc aattttttta acgtgccgtc ttcacgtaat
    21901 tctacgattg ctttgctgat tttcgctaat gcttcgtcat tgcctttatt cgcaattaag
    21961 cctgcaccca ctttttcttc tttttcccac acaattttta agccggaatt cgggttcttt
    22021 ttcaagtaat ctaatagcga taagtggcta ttgaaagttg catcggcgcg tttttgttga
    22081 attaacagta agccttgagc cataccgtca attggcacga tgtctgcacc gttttcacgg
    22141 gcaatctcgc catagtttga gctcaaggtt tgggcagttt tcacgccttt tacatcagca
    22201 ggggttttga ttttagtttc atcttcacgc actaataagg cggcaccgtc ccagctgtaa
    22261 gattcggatt tatcaaatgt cgctcgacgt tccgggctgg ttaaagccac ttggtttgcc
    22321 actaagtcaa aacgctcacc ttttaaaccg gctaacatag cgtcccattg tgtctcttta
    22381 aattccactt ttacgtctaa tttttcagcc accgcacggg ttacttctac atcgtaaccg
    22441 gttaatttac cgctcgcatc gtgataggtg taaggggcat aagttccttc cgtacccaca
    22501 gtaatagtcc ctttgttgtt tatacgctca agtaggctct ctttagcaaa tgcagaaaca
    22561 gaaagggtaa gagctaatgc agcaattgat aattttttca acataatcat ttccttttaa
    22621 ttggtaaaat aaattttttg caaatttaag gggaaaattg accgcttgca aataactaaa
    22681 tggaataaga aataataaaa tcgttgatgt ggttaaaata ggtaatattg agaagatgaa
    22741 ataaaaaaca ccttaataaa ttagcttatt aaggtgctgg tttttaacgc tgacgagctt
    22801 taaatctcgg gttggttttg caaatcacat aaagtttgcc tttgcgtctg acaatttggc
    22861 aatccgggtg gcgtgatttt gcggttttaa gtgagtttaa gattttcatt ttatcctcta
    22921 tttttgattt gaaagcgttg aagcaaagcc tttaaatcta tctttaaatt gacttgcacg
    22981 accttctgta ttcacttcac ggcgtttacc tgtgtaaaca gggtgagacg ccgatgaggt
    23041 atctaaggtg taaagtgggt attctttgcc atcttcccat accattgttt tgttggttgc
    23101 cgcacaagaa cggataaccc agccttgttg cactgaggca tcatagaaca acacttcacg
    23161 ataattttca ggatgaatcc cttttttcat attttctcca gaatttagat aaggtcatta
    23221 accataatta aaatatggga tacataatat acgaacttta aatacaattc cataactttt
    23281 ttatggtgtt ttgtaaaaaa acggattttt aggaagagag actcttttcg ctgttaaaat
    23341 tatggtaatc taacgtagat ttattttttg atagtgagga aatatgagta agaatcctta
    23401 tttaattgca ccgtctattt tatctgccga tttagctcgc ttgggtgatg atgtggcaga
    23461 ggtgttaaaa gccggtgcag acttaattca ctttgatgta atggacaacc actacgtgcc
    23521 gaatttgacc ttcgggccgg cgatctgcaa agccttacgt gattacggca ttcaatgccc
    23581 gattgatgtg catttaatgg taaaaccggt tgagcgtttg attccggatt ttgccaaagc
    23641 cggagcggat tatattacat tccaccctga agcaacggaa catatagatc gaaatttaca
    23701 gcttatccgt gatttaggtt gtaaatcagg cttggtgttt aacccggcaa cgccattgag
    23761 ctatttagat tatgtgatgg ataaagttga cgtggtttta ttgatgtctg tgaatccggg
    23821 ctttggcgga caatcattta ttccgtccac cttagataaa ttgaaagaag tccgtcgtcg
    23881 tattgatgag agcggttata acattcgctt agaggttgat ggtggtgtga aaataaataa
    23941 cattgccgac attgcagccg caggagcaga tatgtttgtg gcaggttctg cgatttttga
    24001 ccagccagat tatcaagcag tgattgcaca aatgcgagat gaattacgga aagtagggta
    24061 atcaaa
//
LOCUS       DANLXQ010000019        23794 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.019, whole genome shotgun sequence.
ACCESSION   DANLXQ010000019 DANLXQ010000000
VERSION     DANLXQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23794
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.019"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            45..2711
                     /locus_tag="PXD94_000604"
     CDS             45..2711
                     /locus_tag="PXD94_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL6285496.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     DGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSRLGLSELGDR
                     VLIYVNIQDKYKHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            2729..3238
                     /gene="luxS"
                     /locus_tag="PXD94_000605"
     CDS             2729..3238
                     /gene="luxS"
                     /locus_tag="PXD94_000605"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL6285497.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGSPSAEEVVK
                     AWTASMEDALHKIPDVSAIPELNEYQCGSYKEHSLKEAHQIARNVLAAGIGINRNEDL
                     ALDEKLLNP"
     gene            3307..5154
                     /locus_tag="PXD94_000606"
     CDS             3307..5154
                     /locus_tag="PXD94_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6285498.1"
                     /translation="MFEKLFKWFEARVETYPNEMPKTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEGFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            5341..6690
                     /gene="dnaA"
                     /locus_tag="PXD94_000607"
     CDS             5341..6690
                     /gene="dnaA"
                     /locus_tag="PXD94_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL6285499.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGELTLY
                     AQNQFVENWVKDKFLSEITELARFLAKNDNLVVTLKVGIKPTEQPKVSPNLKNESSEF
                     SNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHCNPLSFYAGTGLGKTH
                     LLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEKIKKFYRSLDVLMIDD
                     IQFLANKPKVQEEFLHLLNALFEQRKQIILTSDVLPKNIDKIDTAIKSRLSGGISATI
                     EPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELEGALNRVIAWQKLTLR
                     PITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIKSKSRARSIARPRQMA
                     MALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESGIQEDYIHLTRKLSS"
     gene            6709..7812
                     /gene="dnaN"
                     /locus_tag="PXD94_000608"
     CDS             6709..7812
                     /gene="dnaN"
                     /locus_tag="PXD94_000608"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL6285500.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(7875..8495)
                     /locus_tag="PXD94_000609"
     CDS             complement(7875..8495)
                     /locus_tag="PXD94_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL6285501.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(8505..9776)
                     /gene="hisS"
                     /locus_tag="PXD94_000610"
     CDS             complement(8505..9776)
                     /gene="hisS"
                     /locus_tag="PXD94_000610"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL6285502.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALG
                     SQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYIVF
                     SGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESEVA
                     EKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            complement(9782..10894)
                     /gene="ispG"
                     /locus_tag="PXD94_000611"
                     /gene_synonym="gcpE"
     CDS             complement(9782..10894)
                     /gene="ispG"
                     /locus_tag="PXD94_000611"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL6285503.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            complement(10995..11993)
                     /locus_tag="PXD94_000612"
     CDS             complement(10995..11993)
                     /locus_tag="PXD94_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL6285504.1"
                     /translation="MTESVNSTQAGTALSLGQQLKAAREALNLSITDVAQKTNLKKSH
                     IESLENDIFILQNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVK
                     PSSNSQGKTLKYLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATN
                     NDASAQTSVPVQQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVE
                     EVKQADATTAQVEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNK
                     RLAEKLYNNGETLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIHLPLQ"
     gene            complement(12161..13540)
                     /gene="argH"
                     /locus_tag="PXD94_000613"
     CDS             complement(12161..13540)
                     /gene="argH"
                     /locus_tag="PXD94_000613"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL6285505.1"
                     /translation="MALWGGRFTQQADAKFKYFNDSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            complement(13657..14820)
                     /gene="mnmA"
                     /locus_tag="PXD94_000614"
     CDS             complement(13657..14820)
                     /gene="mnmA"
                     /locus_tag="PXD94_000614"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL6285506.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAAELADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRAIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVIDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            complement(14931..15242)
                     /locus_tag="PXD94_000615"
     CDS             complement(14931..15242)
                     /locus_tag="PXD94_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL6285507.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            complement(15224..15640)
                     /locus_tag="PXD94_000616"
     CDS             complement(15224..15640)
                     /locus_tag="PXD94_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL6285508.1"
                     /translation="MNRDIRWKQRFDNYKKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIYGSKTASRLAFNRGLITQGEVWLEMIKSRNITVHT
                     DDETILTEEFNKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            complement(15642..15887)
                     /gene="nqrM"
                     /locus_tag="PXD94_000617"
     CDS             complement(15642..15887)
                     /gene="nqrM"
                     /locus_tag="PXD94_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL6285509.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            complement(15900..16934)
                     /locus_tag="PXD94_000618"
     CDS             complement(15900..16934)
                     /locus_tag="PXD94_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL6285510.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEKPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            complement(16983..17276)
                     /locus_tag="PXD94_000619"
     CDS             complement(16983..17276)
                     /locus_tag="PXD94_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL6285511.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            complement(17269..17703)
                     /locus_tag="PXD94_000620"
     CDS             complement(17269..17703)
                     /locus_tag="PXD94_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL6285512.1"
                     /translation="MEQLDITVLENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            complement(17815..19044)
                     /gene="nqrF"
                     /locus_tag="PXD94_000621"
     CDS             complement(17815..19044)
                     /gene="nqrF"
                     /locus_tag="PXD94_000621"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL6285513.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
     gene            complement(19055..19651)
                     /gene="nqrE"
                     /locus_tag="PXD94_000622"
     CDS             complement(19055..19651)
                     /gene="nqrE"
                     /locus_tag="PXD94_000622"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL6285514.1"
                     /translation="MEHLLSIFVKSIFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            complement(19653..20279)
                     /locus_tag="PXD94_000623"
     CDS             complement(19653..20279)
                     /locus_tag="PXD94_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL6285515.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            complement(20279..21052)
                     /locus_tag="PXD94_000624"
     CDS             complement(20279..21052)
                     /locus_tag="PXD94_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL6285516.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIANPNWQKNFVGKKLYNEQNQVALRVGKGASADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            complement(21045..22280)
                     /locus_tag="PXD94_000625"
     CDS             complement(21045..22280)
                     /locus_tag="PXD94_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL6285517.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            complement(22283..23641)
                     /locus_tag="PXD94_000626"
     CDS             complement(22283..23641)
                     /locus_tag="PXD94_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL6285518.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
BASE COUNT         6955 a         5237 c         4606 g         6996 t
ORIGIN      
        1 tttgggtctt cggtattgtt ctatataggt ttccctaatt taaaatgacg atggaagaaa
       61 atacccacac tgttgtagat gcccaaatcc ggcagcctcg caaaatttct cctttttggc
      121 tgttaccaat agttgctttt attattggtg ctttgctatt tttccaaatt ttaaaagagc
      181 aaggtgaaac aatcacgatt cgatttagta aaggtgatgg cattactgcc ggaaaaaccg
      241 ctattcgtta ccaaggcttg cagattggtc aggttaagcg tgtttatttt gtcgataatt
      301 taaaagaagt ggaagtgcaa gcagaaataa accctgaagc caaaagtatt ttacgcaaac
      361 agactaaatt ttggttagtg caaccaagtg cttctttggc tggcgtttca ggcttagatg
      421 cgattgtttc tggtaactat attaccctac ttcccggtga aggcgatttc gttgatgaat
      481 ttattgcaga agaagatcct ccggctatta ccgtgagcga tggcgattta ctgatccgtt
      541 taatctctga tgacataggc tctattactg tcggagcgag tgtttatttc cgtaaagtgc
      601 cagtgggtaa tattgccgat tatcgtttta ccaaagacca gaaaaaaatc gaaattgatg
      661 tcgtcattaa tcaaaaatac gctcatttag taaaaaaaga gagccgattt tggaatatta
      721 gcggtattag cgctgatgta aatttacaag gtattaaaat caatatggac agccttgcct
      781 cggtagtaca aggtgcggtt gcttttgatt cgcctgataa tatggaaatt gccgagcaag
      841 gacagaaatt tgagctatat ccggatttga aatcagctaa acgtggaatt gaaatcaacg
      901 taaaactacc gcttacacat aatttaaaaa ttaatgagac tggtgttttt caccaaaatg
      961 tgcaaattgg tatgctttcg cagctaaaat taccgcaagt aaaagacaac ggtgtgccac
     1021 agcaagaatc agggcagcat aaacagattg atggggtttt attaattgac ccaagttatg
     1081 aaaatttact acgttcccaa actaaaatct tgctcaaaga gccgaaattc tccttaaaca
     1141 cagagcagct gacgaaagta ggtgagctgc tgcgtggtgt ctattttgag gttcacccgg
     1201 gggaaggaga acctcaatcg gaatttgtgg tacaaaacga agccgattat ctattaagtt
     1261 taccgaattt acttagcttt agcttaattg ctcctcaagc ctatggggta gataccaatc
     1321 aggggattta ctatagcgat gtgcaaatcg gagaaatcgt caaacgtagc ctagagttag
     1381 acaaagtacg ttttgatgtc attatttatc cgccttatcg caacctgatt gggagtaaca
     1441 gcaaatttgt tgcgatatcc aacattgata tgtcggtggg gctagatggg ttaagagttc
     1501 acgccggttc accatctgaa tggttaaaag gcggtgttcg cttaattaac ggtaaaaatg
     1561 acggtagccc gaaaaaacaa tatgcacttt ataaagatat agaaagtgcg gaatcaggca
     1621 tggctacctt agataaaaaa gccaccacta ccctttcggc agattcatta tctggtatca
     1681 gcgaaggctc tttggtgcta tatcgtgatt ttcaggtcgg cgaggtgtta aaaataaccc
     1741 cgaaacaaca aaaatttgag gttgagctat ttatccagcc aaattatcgt catctattaa
     1801 ccgagaaaag ccgtttctgg attgagccgg caacgagtgt tgatgtgtca ctcaaaggcg
     1861 tgaatatcac tacagctcca ttaatgcgta ccttaaaagg ggcgatcagc tttgatgatg
     1921 gcggttcaaa aggcaataaa acgctttatg caagtaagga gaaagccact tcgggcaata
     1981 cttatctcac gctgattgca aaagatgcga gtaagttaag cgaaggaatg gacattaaat
     2041 atatgggctt aaccgtaggt caaattgaaa aacttgagct acaaaatgca aaaaaacaga
     2101 taaaagcgac cgcttacatt caaaggaaat actatccgtt aattgcaaaa gccggcagta
     2161 aatttaatgt tatctcgcct gaaatcagta cctccggctt taaaaattta gatgcagcac
     2221 tacaaaatta cattacggtt gatgtcggtt cagggcaagc tcaaaaccaa tttgttcttg
     2281 cagataccga tacggtaaaa accacctatg ccaacggttt ccccattatt gtcgaaacca
     2341 ccaatgctaa cggtattcag ccggaagcac cggtgcttta ccgtggaatg caggtgggaa
     2401 tagtcagccg tttagggcta agtgagttag gcgatcgagt gcttatttat gtcaatattc
     2461 aagataaata caagcactta gtcagaacca atacccaatt ctggcaagct tcaggttaca
     2521 ctatggacgt gagcctgcaa ggtgtaagta tgaactccgg cacaatgtcg cagttattaa
     2581 atggcggtat tgagttctct accccttaca ctaaagtggt gaagcctcaa gcccagccaa
     2641 acagacgatt tttcttacag cgcaaactac ctgatgaagc cccggcttgg gatcaaggaa
     2701 ttgcggaata atattgatgg agtaaaaaat gcctttatta gatagtttta aagtagatca
     2761 caccaaaatg aacgcaccag ccgtgcgtgt tgcaaaaaca atgaccacac cgaaaggcga
     2821 taccattacc gtgttcgatc tccgtttctg tcgtccgaat attgatattc taccggtgcg
     2881 tgggattcac acgatggaac atctttttgc cggttttatg cgtgatcacc tcaatagcga
     2941 acaagtggag atcattgata tttcaccaat gggttgtcgt accggttttt atatgtcact
     3001 gattggatcg ccttcagcag aagaagttgt caaagcttgg acggcatcaa tggaagatgc
     3061 gttacataaa attcctgatg tatcggcaat ccctgagctg aatgaatatc aatgtggctc
     3121 ttacaaagag cattctttaa aagaagcaca tcaaattgcc cgcaacgtat tagctgccgg
     3181 cattggtata aatcgcaacg aagatttagc cttagacgaa aaattactta atccataatt
     3241 ttttcacatt ttttcggggg caaatggcta tttgcccctg catttttatt cggaaatcac
     3301 aaatttatgt ttgaaaaact gtttaaatgg tttgaggcaa gggtggaaac ctatccaaat
     3361 gaaatgccga aaactccaaa atcaggtctc attcctttta tttacgatgc cactaaagga
     3421 atgcggtttt atatcttact actgaccgta ttagttgcag ctgtcggtat tatcgaagcg
     3481 gttctgtttc gctttatggg cgatttagtc gattgggtca ataaatactc gccaacagaa
     3541 ctttgggcag aaaaaggctg gacgattatc ggtatgtttt ttattgccct acttgcagtg
     3601 gtgtttgttt accttgccag cagcattcgc tttcaatctc tgcaaggggt atttccaatg
     3661 cgtttgcgtt ggaacttcca ccgcttaatg cttgggcaaa gtttaggttt ctatcaagac
     3721 gaatttgcag gaagagtgtc tgcaaaagta atgcaaaccg ccctttccgt gcgtgatgtg
     3781 attatgactt gtgccgatat gttggtgtat gtgctggttt acttcaccac ctccagcgtg
     3841 attttattac aacttgacgg ctggttattc gtgccgttta ttttatgggt catctcatta
     3901 gcggcaacca ttatctactt tgtcccgaaa ttagcccaag ctgctcaaga gcaatcggat
     3961 gccagaagtt taatgaccgg tcgaattacc gatgcttacg ctaacattgc caccgtcaaa
     4021 ctcttctcgc acggcaaccg tgaatctgcc tatgcgaaag agtcgatgga aggatttatg
     4081 gtgacagtgc ataaacagat gcgtttagtg actgtaattg aaaccgtaac taatctaacc
     4141 tcgatggcat taataatttc aactgcgggt atcggtttat ggctttgggg ttcaagcgta
     4201 gtaactgcag gggcgattgc cacctcaacc gctttggctt tacgcattaa aggcttatca
     4261 caatggatta tgtgggaatt tgctcgtcta tttgaaaatt taggtacggt acaagacggt
     4321 atgattacgc tttccaaacc acacaatgtg gtagataaaa tcgatgcgaa accgttaaat
     4381 gtaaccaaag gcgaaattaa atttgagaat gtggattttg cctacgatcc gaaaaaaccg
     4441 ctactgaaag attttgacct cactataaaa ccgggggaaa aagtcggctt agtcgggcgt
     4501 agtggtgcag gtaaatcaac gctcaccaat ttattattgc gtttttacga tattcaagac
     4561 ggttcaatca cgattgacgg gcaaaatatc cgtgatatta cccaagaaag ccttcgctca
     4621 caaattggtt tagtaactca agacacctcg ctgttacacc gctctgtgcg tgaaaacttg
     4681 atgtacggtc gcccaaacgc cactgaagaa gagatgatca aagccgttga acaagctgcc
     4741 gccagtgaat ttattccaaa cctgcaagat gccaaaggca gaaccggttt tgatgcacac
     4801 gttggcgaac gaggggtaaa actttccggc ggacaacgcc aacggattgc gattgcccga
     4861 gtgatgctaa aagacgctcc gattttatta ctcgatgagg caaccagtgc gttagactct
     4921 gaagtagaag tggcaattca agaaaatctc acgcaattaa tggaaggtaa aaccgtggtt
     4981 gcgattgccc accgcctctc taccattatg gcaatggaca gacttatcgt gttagataaa
     5041 ggcgaaatcg ttgaacaagg cacgcacgaa gaattgcttg ctcaaaacgg tgtttacgcc
     5101 agactttggg ctcaccaaag cggtgggttc ttgccggaga atacggatat ttaatcaact
     5161 ccatcacaca tcatactaag caagcggtca ttttttgtta gcaatttgca agatttcaac
     5221 aaaaaacgac cgcttgttcg ccaattaaaa tcgcttccaa gcccccataa aatctctcaa
     5281 aaatttgcaa gatttctccc cttttttcag gtatcattat gacctttaat aacaataatt
     5341 ttggagcaaa ttgtgtcttc cctttggtct gattgcctta atcacttaca aacgaaagtc
     5401 tcaccaactg attacagcac ttggctgcgt cctctgcaag cgagttctga gaatggggaa
     5461 cttacacttt atgcacaaaa tcaatttgtt gaaaactggg taaaagataa atttttatcg
     5521 gaaattaccg agcttgcccg ttttttggcg aaaaatgata atttggtggt tacgttaaaa
     5581 gttgggatta aaccgacaga acagccaaag gtcagcccaa atctaaaaaa cgagagttca
     5641 gaattcagca ataccggctt taaaaccggg ctaaatccaa atcaaacctt tgataatttt
     5701 gtacaaggtc gctcaaacca attagctaaa ttagtggctc aacaagtggt ggataattta
     5761 ggcgaaagcc attgcaaccc actctcgttt tatgccggca ccggtttggg caaaacccac
     5821 ttattacacg ctatcggcaa tgaattagtt aaacgcacac cgggtactcg ggtgctatat
     5881 attcatttgg aacgttttta tcgtgatatt attaaggcga tcaacagttc caataacgat
     5941 gcggaaaaaa tcaaaaaatt ctaccgctcg cttgatgtgt taatgattga cgatattcaa
     6001 tttcttgcca ataaaccgaa agtacaagaa gagtttttac atctactcaa tgctttattt
     6061 gagcagagaa agcagattat tctaacctct gacgttttgc caaaaaatat cgataaaatt
     6121 gacacagcga ttaaatcccg tttaagcggc ggtattagtg cgaccattga gccacctgaa
     6181 ttggaaaccc gagtggcaat tcttcgccaa aaagccttag aacgtggagc agatttgcaa
     6241 gaagatgccg cctatttaat ggggcaaaaa ctccgaacca atgtgcgtga attggaggga
     6301 gcattaaacc gtgtgattgc gtggcaaaaa ttgacccttc gcccgattac ggttgatgcg
     6361 gtgcgtgaaa ccttgcaaga tctgtttgcc tcttttgatt acttgattac gattgaaaat
     6421 attcaacgca ttgtggccga atattacggc ataaaagttg ccgatattaa atcgaaaagt
     6481 cgggcaagaa gcattgcccg tcctcgccaa atggcaatgg cactggcaaa agagctaacc
     6541 aaccacagct tgccggaaat tggtcgggaa tttggcggtc gggatcatac taccgtgatg
     6601 cacgcctgca aaaccattaa tgaattacgc gataccgaaa gcggtatcca agaagattat
     6661 atccatttaa cacgcaaatt atcatcttag cccgatgaag gaatgattat gcaaattacc
     6721 attaccagag aacaattact taagccttta caacaggtgt gtggcgtatt aagtagccgt
     6781 ccgactttgc ctgttattaa caatctttta ttagaaattg aaggtaatcg tctatcttta
     6841 accggtaccg atttagaagt ggaattaacc acccaagcac aactgctaca agcggtcgat
     6901 tttgccggaa aatttaccat tccagctaaa aaattcctag atatcagtaa aagcctacct
     6961 gatgacagcg tgatttcggt acaatttgaa gaagataaag ccattatcaa agcaggacgt
     7021 agtaaattta gtttagctac attaccggca agtgattacc cgaatttgat ggattggaaa
     7081 ccggaagtgg atttctcgat tgatcaatct acccttgccc gtctgattga tgccacccaa
     7141 ttctcaatgg caaaccaaga cgcccgttat ttcttaaacg gaatgaaatt tgaaaccgaa
     7201 ggcaatttat tacgcaccgt tgccacagat gggcaccgtt tagcggtttg cacaatggtg
     7261 ctaaatcaag agcttttgac tcattcggtt atcgtgccac gcaaagccgt acttgagctt
     7321 tcccgcttgg ttgccagcaa cgatgaaacc gtgcgtttgg aaatcggttc aagcaatatc
     7381 cgtattacaa tgaacggcat tgtatttacc tctaaactga ttgatggcag attcccggat
     7441 taccgccgag ttctgcctcg caatgcagat cgcattctag aagcggaaac cgaaaccctt
     7501 aaacgggcat taatgcgtgc agcgattctt tctaatgaga aatttagagg ggtgcgttta
     7561 accttaagca ataatttact caaaattacc gcaaacaatc cggagcaaga agaggcggaa
     7621 gagatcattg atgttgccta cgacagcccg gaaatggaag tcggctttaa cgttagctat
     7681 gtattagatg tgttaaacac cttaaaatgc caacgtgtgc gttttagctt agttgattca
     7741 agttctagtt gcttaattga agattgcgac aacagctctg ccgaatatgt gattatgcca
     7801 atgcgtttat aattcacagc aacactaaac caacaaaaaa gcctgattga ttttatttca
     7861 atcaggcttt gtttttataa gttattcaaa cgtacatcaa ttaaggtttg ttcctgagga
     7921 tttgcggtcg ttttagcttg ctcataagca cttttcgccg caggaatatc gccttttgct
     7981 gcaagaatat cgcctgttag aatttgctta cgcaattccc acgctttatc ttgaatttga
     8041 gccagacttg ccagtgcttc atcgtaagat tttagttgga agtcaatttg agccaaacga
     8101 aaacgagcga tagattgtaa ggttgaatcc gaataagtca aaactgactg taacgctgtt
     8161 tttgccgcag aaaaattccc tttttcgacc gcttgtttag ctacttcaaa ttgggcaaaa
     8221 gcggcatagc ttgaatcttt attatcggca ataaatttct caacaagcgg ttgatttttg
     8281 cttggatttt gcaaataact ctccatcacg ctttggtatg aagcggatgt tttttgtgcc
     8341 gtttcaagct ggtgattttt ccaaaaattc cagccggcag ttgccgcaca agctaaaata
     8401 atcgccacca aaatcggtgt cccgttctct ttaaaccaat ttttggcatc ttcaaattgc
     8461 tgctcttcgg ttttatttaa atattcgtta ctcatctttt tctcctaaaa tcgtttagat
     8521 agctcagcaa tcagctctga ttgagcaatc gtgatttgtt ctgctcctga acgtaaatct
     8581 ttgataacaa cttgcttttc agccacttcg ctttcgccaa tcactaacgc aatttgcgct
     8641 tctactttat ctgcacgctt aaattgtttt tggaatttac cgccggaaca gtgtgtcatt
     8701 acgccaacgt gtggcaattc gctacgaatc tgctctgcaa gttggaaggc atttagtgtt
     8761 gccccttcac cggagaatac gatatacaca tccaccgttt ttggtaaatc aatctcttta
     8821 ttcacctctt gtaccaacag cactaaacgc tctaagccca ttgcaaagcc aacgccttgg
     8881 gttgcgtgtc caccgagttg ttcgactaag ccgtcataac gcccaccgcc acatacggtg
     8941 ccttgtgagc ctaaggctga agtgacccat tcaaacacgg ttttattgta atagtctaaa
     9001 ccacgcacca attttggatt gacttcgtaa cggattccca ttgcatcaag taaggcacaa
     9061 agttgggtaa aatgttcacg gctgtcatca tctaaataat cgagcagttt tggggcatcg
     9121 tttaatacat tttgtagtgc ttcgtttttg gtatctaaaa tccgtagcgg gtttttcacc
     9181 aaacgctctt tctcttcttc gttcattaaa gcggtgtgat tttctaaaaa ttttactaat
     9241 gctgagcgat aatttgcacg agcttctaac gagccgatag agttaagttg caaagaaaca
     9301 tgttgatcga tgcctaactc tttccatagg cgtgcggtga gaataatcaa ctccgcatca
     9361 atttccggat ttgcaatgcc aaacacttca acaccggctt ggtggaattg gcggtaacgc
     9421 cctttctgtg gacgttcgtg gcggaacatc ggccccatat accataagcg ttgctcatta
     9481 ttgtagatcc aaccacgctc aatcgctgca cgcacacaac cggctgtacc ttccgggcgg
     9541 agcgttaatt gctcatcgtt atcccagaac gtgtacatct ctttcgacac cacatcggtt
     9601 acttcgccaa tggcacgggc aaataacgga gtgctttcta caatcggcat acgtacttcc
     9661 gaatagccgt aacttgccag cacattacgc actttctgtt caacccattg ccataatgga
     9721 gactcacttg gtgagcagtc gttcattcct cgaattgctt gaattgtttt tgccactata
     9781 tttatcctat ttgttcaata tctatacgat tatgttgttg tgcaactctg gcacgaattt
     9841 tagcttctaa ttggtcgatt aattttgcgt tgtcaaaacg ctctttttga cgaacgccat
     9901 ctaaataaaa gccgctcatt ttattgctgc cggttacccc taaatcagac accaacgcct
     9961 ctcctggacc gtttaccaca cagccgataa tcgaaacgtc catcggcgtg ataatgtctt
    10021 ctaaacgttg ttctagctcg tttaccgtcg caatcacatc aatttcttgg cgggagcagg
    10081 ttgggcaagc aataaagttg atcccacgag agcgaatacg cagcgatttc aaaatatcaa
    10141 aaccgacttt cacctcttca acaggatcag ccgctaatga cacacgcaag gtatcaccaa
    10201 tcccttccgc tagcaacaga cctaagccta ccgccgattt caccgaaccg gctctcgctc
    10261 cgcctgcttc agtaatacct aaatgcaggg gctgatcaat ctgttttgca agaagtcggt
    10321 aagattcaac cgctagaaat acatctgagg ctttcacgct gactttgaat tgatcaaagt
    10381 taaggcgatc taagtgatca acgtggcgta atgccgattc taataatgcc tgtggtgtcg
    10441 gctcgccata tttttcctgc aaatcacgct ctaatgatcc ggcatttacc ccgattcgga
    10501 tcggaatgtt tttatctctg gcacaatcta ccaccgtgcg gatccgctct tcattcccga
    10561 tattacccgg gttaatccgc aagcaatcca ccccatattc ggcaactttc aacgcaatgc
    10621 ggtaatcgaa gtggatatcc gccaccaatg gcacactcac ttgttgttta atcgctttaa
    10681 atgcctcagc agcgtccatt gtcggcacag acacacgcac aatatcagcc ccgacccgtt
    10741 ccagcgattt aatttgagca acggtggctt ccacatcggt tgtgcgggtg ttggtcattg
    10801 actgaaccgt aatcggtgca tcgccgccta caggcacatt acccacatag attttcttcg
    10861 atacccgacg tttaataggt gattgatgta tcatattttt agctaattgt gaaaatttat
    10921 aaaaacagaa aacgctcgta aagacagcta tcgttaaatg ctagctttac gtgcgtttca
    10981 tcgttaaatc aagcttactg taacggtaaa tgaatacgag ctacacgccc atcaattttc
    11041 aatggtactt cttgaccttt atagtacact tttacattgg caggcgcacc aatggttaaa
    11101 cgatattgct cattctcatt gaaacttaaa gtctcaccat tgttatataa tttttctgct
    11161 aaacgtttat ttttagcacc ccgcactgta atccagcttt gtgaaccggt aatttcgata
    11221 cgaagctcat cattcgttac cgcaacaggc tgttctgtag gggcttgttc cgattgagat
    11281 tgctgtaaaa cattaactgc ctcaacctgt gcagtggtgg catcagcttg tttaacttct
    11341 tctaccactt taacatcttc ttgtgttaca ggtgatgatg ctgcttgtgt aggctctact
    11401 ttttcagtta atgccggttc tgctgcaact accgtttctg ctacttgagc aggctgctct
    11461 gaaacctgaa taggttgctg aacaggcacg gaagtttgtg ctgaagcatc attatttgta
    11521 gcggtattcg ccacttcagc aactggtgca gagctaacaa attgctcgcg attttcctgt
    11581 tctttttgat acccttgcca ccaccaaagt aatgtcattc ctaatgccgc aagcaatact
    11641 aataccgtta aatactttaa ggttttacct tgtgaatttg atgaaggttt tactgaagaa
    11701 actttagtga cttcttttgg aatagtgact tcaccataat taacgctcga aataagggat
    11761 tcaggtaaac gcaaaaattt cacataatta cgcacatagc cacgcacaaa agttggggca
    11821 acattttgta gaataaagat atcattttca agtgattcaa tatgtgattt ttttaagttg
    11881 gttttttgag caacatctgt aatagagaga tttaacgctt cacgagccgc ttttaattgt
    11941 tgccctaaag aaagtgctgt accagcttga gtagaattaa ctgattccgt catataaaac
    12001 tctttaaaga aagtagtaga aataaaattc aaaaattatt cggaagttta aagaggtaat
    12061 gcctattttg caatcttaat gcgtaaaaaa ttgaaaattt aatcggcaag cggtcatatt
    12121 tttcaatttt tttgcaaaat atgaccgctt gtatccggtt ttactttgaa gctaaattgg
    12181 ctttggctac cgcaatcgcc tctgccacac gttgtgggtt cactccacct ttggcacaac
    12241 gtttttctaa gcaagattct aacgagagaa ttgggtaaac atcatcgcca atcgaatgat
    12301 ggaattgctg gaattcgtta atgcttaatg cttcaagtgg ctcttttttg ctgattgcgt
    12361 aaaccaccgc ttcgccaaca atatgatgag cttcacggaa tggcacgcct ttcgccacta
    12421 aataatctgc caactcggtc gcattcgcat aaccttgctg agcggcttct cgggtgcgtt
    12481 cggtattcac ttgaatatca accagtacaa gttctgcaat ttctaaacaa gcctgccaag
    12541 tttccaacgc atcaaaaata ccctctttgt cctcttgcat atctttatta taagcaagtg
    12601 gtaacccttt gagtgtagtt aataaaccgt ttaatgcccc aaatacccga ccggatttcc
    12661 cacgaatcag ctcacaagca tcagggtttt tcttctgtgg cataagtgat gagcctgagg
    12721 tcacacgatc cgacatttca aggaagcctg attcgccact attaaaaatg attaaatctt
    12781 cagcaaagcg agagaggtgc atcatactga tcgaggcagt agagagcagc tctaaaatat
    12841 ggtcacgatc tgacacgctg tctaaactgt tgcgtgtggc ttcttcaaag cctaaatcac
    12901 gggctaataa gtcacgatca atgccgtaag ccgtaccagc caatgcccct gaaccaagcg
    12961 ggcaagtgct catacgttta ttagcatcac tcaaacggct gaaatcccgt tctagcattt
    13021 cataatacgc catacaccaa tgagcgaagg tcacaggctg agctcgttgt aaatgggtat
    13081 agcccggcat caccgactct tgattttctt cggcaacgga aaccaaacgc tcttgcaaag
    13141 ctcggattct ttcttgcaat gccaccgctt gcaccttgca ccacatttta atatccaccg
    13201 ctacttggtc attacggcta cgcccggtgt gcaatttttt acctaaatcg cccacttttt
    13261 caatcagctt agattcaacc cagctgtgaa tgtcttccgc atcatctttt aagatgatgg
    13321 taaggttaga ttctacctct gctctcacct cttttaatgc tgcgattagt tgttctaatt
    13381 ctgctaccgt taaaatacct accgaagtaa tggcttttgc ccagccgatt gagccttcaa
    13441 tatcttgaat cgccaagcgg taatcaaaac gtaatgaatc gttaaaatat ttaaattttg
    13501 cgtctgcttg ttgtgtaaag cgtccacccc aaagtgccac agtctgttcc ttgtatatta
    13561 ttctttgtat cgatcaaaat gaaaaaggcg ttttaccgcc ttaacttttc gcttattttg
    13621 cataaatatg caaaatattt caatatttat ttataactat tttaattgtg actcaataat
    13681 gccaccgcct aagcaaactt cacctaagta aaacacggca gactgccccg gcgtcacggc
    13741 aatttgcggt tcatcaaaca tcactcgaat ggtttcatca tcaatcactt ggatttcaca
    13801 cgcaatatct gcttgacgat aacgggtttt taccgtgcag cgtaaatttt cacgcaacgg
    13861 ctgcatatcc acccaagtga gctgtttcgc aattaagcca cttgaaagca gggctgaatt
    13921 atcgtgtcct tgtgccacaa tcaatacatt gttaattaag tctttctcca ccacataaaa
    13981 cggatcttca cttagccctt tcaccccacc gatacccaag cctttgcgtt gcccaagcgt
    14041 gtgatacatc agcccatcat ggcgaccgac taatttgccc tcaacggtgc ggatttcccc
    14101 cggttgagcc ggtagaaaac gagctaagaa atctttaaat ttacgctcac cgataaaaca
    14161 aatgccggta gaatcttttt tctttgcggt tgctaatcct aggtcttcgg caatggctcg
    14221 cacaatcggc ttctcaattt cacccactgg gaataaactc tgcccgatct gctttttatt
    14281 taacgcatat aaaaagtaac tctgatcttt gttttcatct aagccacgca aaagtttcgc
    14341 atcttgatca tcgctcgtac ggcggacgta atgcccggtc gcaatataat ccgcaccaag
    14401 atcttctgcc gcatattcca aaaaggcttt aaatttgatc tctttattac acaaaatatc
    14461 cggatttggc gttcttcctg ccttgtattc tgccaagaaa tattcaaata cattatccca
    14521 atattccgct gcaaaattaa ttttatgcag tttcataccc agtttatcgg caaccgcttg
    14581 agcatcggca agctcggcgg cggcggtaca ataatccgta tcatcatctt cttcccagtt
    14641 tttcataaac aagccttcca cttggtagcc ctgctgttga agaataaaag cggaaaccga
    14701 ggaatccacc ccaccggaca taccgataat gacttttttc ttgctgtttt cagcaagttg
    14761 ttctggggtt aatttaggga aatgagtgtc gtaggttttt gaggttaatt gagttgtcat
    14821 aaatctcccg taacttcggt taaggcgaag cacattggtt aataataaaa acaacaagcg
    14881 gtgaaatttg caaaaaattt tgcaaaatga accgcttgcc tattaagaaa ttagagttct
    14941 ctttggtaaa agacttttcc tactctatca atatgttcaa taaagttggc attggttaat
    15001 tgatgataaa tcgacaagtc aaataaatag ggcgagtcgg aatcgtctaa atcaccccaa
    15061 atgcgtttga caatatcgta agtaagatct tttcctttaa aagtaagatc tacatctgag
    15121 ccttcacgat aattaccttt agcacgcgag ccgtaaataa tggcttgctc cacttcaggg
    15181 tatttcgcaa gaatatcggt aatagttttc gtcgctcgtt cgcttagtcc aaagtttggc
    15241 atagtgtttc tactcgctgt tcaaattcaa taaataaagg cagatacgcc ttgataattt
    15301 tattaaattc ttcagttaaa atagtttcat cgtcagtatg aactgttata ttgcggcttt
    15361 ttatcatttc tagccaaact tcgccctgag tgattagccc acgattaaaa gccaaacgag
    15421 aagccgtttt tgaaccataa atatcaactt caccttccca ttttaaaata tcttgcatta
    15481 acttccacgc taactcgtgt gtaatctcaa aactctggat aattcctttt ttaattacat
    15541 caatgtcggt atcagcgtat ttttctgctt ctgcttgaag gtaacttact gattttttat
    15601 agttatcaaa ccgctgtttc cagcgaatat ctctattcat attatttcac ttcataaaat
    15661 tgtggctctt tattaaaacg tgccacttct tcagggtcgg ctgtgccgtc tgcgatttta
    15721 gattttaggt tatcgcaagg ttcttcgcag tcgcacattt ttttcatacc taaagcggtg
    15781 ataccgccac agctgccttt gatggttttg cctttcacga taaaaccaat cgacatcgca
    15841 aaaattacgg ctacaaaaaa gccaaaggtt aataatacag tttccatttt tcactccaac
    15901 tattgtgatt gaataagttt gctaaattcg ctcgacattt gtgtctcaaa tttatcgccg
    15961 tttttaacaa ttaaaaaaat agctaatttt tcacgctctg ccactgctaa tgctttttcc
    16021 gctcctaaaa caaataaacc ggttgataag ccgtctgccg tcattgtagt tggggcaaat
    16081 acggtgatag aggctaaatt atggtttacc ggacgtaact gttttgggtc gataatatgt
    16141 gaaagacggt tgcctttttc atcttcaaaa taattacgat aattgcccga agttgccatc
    16201 gccaaattgt gcaacggcac ggtaatttgt gcggcttgac cttgtgtcat taccggcttc
    16261 tcgattgcaa tccgccaatc taagccttgt aaattttttc ctttgcctcg taattcgcca
    16321 ccaatttcaa ctaaatagtt gctcacacct atagtttcaa gatgctcggc taatttatct
    16381 acaccgaaac cctttgcaat agacgataaa tctagataaa gattagctgc tgacttggtg
    16441 agcttgggtt tactactcat ttcaagcaaa attttatcaa tgccgacata agcagaacgc
    16501 tctgcaattt gttcatcaga cggcacttta tttaagcgtt tatccggccc gaagccccac
    16561 aagttcacca atggaccaac ggttacatct aaagcccctt cggttacttt atttaaacgg
    16621 attgcctctt gtaccacttt tgcaaaatcc tgcgaaattt cgaccgcttg atttgcctgc
    16681 tttgcttgat taaaacggct aatttccgaa tcttgttggt aggtggacat ctgattattc
    16741 acctcaatca ataagccatc cagctcattt ttcatttttt caggcgaaat caaattctcc
    16801 acttcaccat tatcaatata ttttacggtg taagtcgttc ccatggtttt gcctgacagc
    16861 gtaatttgct caggggcttt ttcacaagca cttaaagtaa aaaaagcaat taaaagaaga
    16921 aaaaattttt tcattggtgt cacaaaagtt aaaaattggg gaaattatag catatttagc
    16981 gattaaataa gcacttcata tcgctgtaaa ataatttgtt taaacgattc gttgcaactt
    17041 gcccagtcaa tcagatctac tgtgaaaggt aggtcagaat ccgtaaacgc aagctctaac
    17101 tctgtttgtt gccgaattgt gagcggttgt ttggtgatta atgctaaatc aagatccgaa
    17161 taggctttgg ctgtgccttt aatgcgagag ccaaaaaccc aaacgggtgt atcaggcaag
    17221 tattttttga aaatatccaa cacctgttgg cgttgtgact cagttaaatc aatcatagct
    17281 attgcgtttt tggagctgtt taactaaaaa atacgtactt tccagaaagt ttgaaattcc
    17341 gttataaacc tgctgtgctt tattttgatc ataagtatgc gaagtaatat ttcgcatttt
    17401 gcgataatcc agccaaactt ccggtgattc aatcagccca tattgagcag ccattcgcaa
    17461 aatatcacgg aactcggtgc tatcaatatc agtgtcattt gactcaatgt ttttgagctg
    17521 acgcttgagc atttttatcg acaattcgta aacaaattca aatttttgga ttgctcctgc
    17581 aatcaaagtg tcttgaataa ttgcttcttg ccggttaaac cacgcttgat tctcaaggct
    17641 gacaatcgtt ttctcaaggg aattgaaggc attttcaagt accgtaatat ccagttgttc
    17701 catttgcttc cccttaaaat tcacattatt aaattaccaa aacttaataa tataaattaa
    17761 atcaagcggt caattttgca aatttgaccg cttgtagtca gaagttaagg tttatcaacc
    17821 accaaagtca tctaataaga tgttttcgtc ttctacacct aaactcttca gcataccgat
    17881 taccgcagca ttcatcaccg gtggaccaca catatagtat tcgcagtctt caggtgcttc
    17941 gtggtttttg aggtagttct cataaagcac gttatgaata aagccacgga agtaatcttc
    18001 tctgtcgccc ggtaatggat cagaaagtgc cacataccat ttgaagttat cgttttctgc
    18061 ttgaagacca tcaaaatctt ccacatagaa catttcacgc tcagaacgag caccatacca
    18121 gaatgacatt ttacgtttag attttaaacg ttttaattgg tcgaagatat gtgaacgcat
    18181 tggtgccata cctgcaccac caccgataaa gaccatttcc gcatcggtat ctttagcgaa
    18241 gaactcaccg aatggacctg aaattgttac tttatcaccc ggttttaatg accaaatgta
    18301 ggaagacatt tgacccggcg gtacgtccgg attgcgtggt ggaggtgtag caatacgcac
    18361 gttaagcata ataatgcctt tctcttccgg atatgaagcc attgagtatg cacgaataat
    18421 atgctcgtcc acttttgaag tataacgcca taagtcaaat ttatcccagt cttcgtggta
    18481 ctcttttgga atatcgaagt ctttataatg aacggtatga ggttcagctt caatttggat
    18541 ataaccaccc gcacggaacg gtacttcttc gccttcagga atttgaagtt taagctcttt
    18601 gatgaaggtt gctttgttat cgtttgagat aacggtacat tcccattttt tcacaccaaa
    18661 gatctcttcc ggaagttcaa cgtccattga agatttcacg tttacctgac aagctaaacg
    18721 ccagccttct ttcgcttctt tcttagaaat gtgcgataac tcagttggaa gaatttcacc
    18781 accgcctgat ttcacttgta ctttacattg accgcaagag ccaccgcccc cacaagctga
    18841 cgaaacgaaa atccctttac ttgctaaagc accgagtaat tttccaccag ccggtagcgt
    18901 aatgcctttt tccggatcgt tgttgattga gatggtaata tcacctgaat caactaattt
    18961 tgatttagcg aaaagaatga tcaccgcaag cactaatacc agagccgtaa atgcgataat
    19021 accgaaaata aaattactat ccacgatatg ctccttataa ttgaatgcct gagaatgaca
    19081 taaagcctaa cgccattaag cctaccgtaa taaaggtaat acctaaacca cgtaagcctg
    19141 ccggcacgtc ggaatatttc attttctcgg ttaaacctgc aagggcaacg atggctaaca
    19201 tccagcctgt acctgcacct acgccataaa ccacagattc tacaaagtta tactcacgtt
    19261 gtaccataaa cgatacacca ccaaaaatcg cacagtttac ggtgataagt ggtaagaaga
    19321 tacctaatgc actataaagt gccgggaaaa acttgtctaa aatcatttcc agaatttgaa
    19381 caagtgccgc aataaccccg atgaaagtga taaagtttaa gaagcttaaa tccacacctt
    19441 ctactaatgc accctctttt aatacgtggg tataaactaa ttggttagct ggaactgcaa
    19501 tgccaagtac tacaattacc gcaataccta aaccaaaagc agttgaaact ttcttagaca
    19561 ccgcaaggaa tgtacacata ccaaggaaga aagaaagtgc catattttca ataaatatgg
    19621 acttaacaaa gatactcaga agatgttcca tcttatttct ccacttgttc cggtttccac
    19681 gttctaatcg cccagatcac aaatccgata ataaagaacg cacttggtgc taataagaat
    19741 aaaccgtttg cttgatacca accgccattt tgaacagttt caagaatggt gataccgaaa
    19801 attttacctg aaccgataag ttcacgtaaa aaggcaacga taattaacat tgcaccgtag
    19861 cctaaaccgt taccgatacc atcaacaaaa ctttcaaccg ggcctgattt catcgcgaat
    19921 gcttctgcac gccccattac gatacagttg gtaataataa ggcctacgaa taccgataat
    19981 tgttttgaca gatcgtaagc ataagcgcgt aagatttggt caaccaaaat tactaatgat
    20041 gcaataatcg ccatttgcac aatgatacgg atactatttg gaatatagtt acgaatcatt
    20101 gaaatgaaca tacttgagaa tgcagtaact aaacttaccg caatcgccat tactactgct
    20161 gtttgtaatt gagtcgttac cgctaacgca gaacaaatac ctaagatctg taatgcgatc
    20221 gggttgttat cgacaattgg cgataacaat aattttttaa gattgttatt gtctgccatt
    20281 agttagctcc tgccgctgct ttaaattttg ctaaatatgg accaaaacca ttttgaccaa
    20341 accagtattt gaatgaacca tcaacaccgt tcgaggttaa tgttgcacca gataaaccat
    20401 caacaccgtg atctttatct gcagatgccc ctttacccac tcttaacgca acttggtttt
    20461 gctcgttata cagtttttta cctacaaagt ttttctgcca gttcgggttt gcaatttcgc
    20521 cacctaagcc tgccgtttca ccttgttcat agtaagtaat accgttaacg gtatttgcat
    20581 caggttgtac cgcaacgaaa ccgtacataa ttgaccataa gccgttaccg tgcattggta
    20641 atactacttg agtcgttttg ccagattcat cttttactaa ataaacttca gcatatttag
    20701 cacgcgtttt gatattcgcc ttatcatctt caggtgcgat aactacattt tgagcaggat
    20761 ctttagctgc tgcacgagca tcaaaaccgg ttacaccctc aacgtaatca cctgttgcta
    20821 aatcgacaat tttcggctca ataaatttgg tataggtttc ttgcacattg gtatcttttt
    20881 gcaataaacc ggccgcttgt aagatgtttt tctgtttatc aagttgtttc tgaatatctt
    20941 gtgttggttt aagcattact gccgcacctg ccacaatcaa agaacaaatt aaacttaata
    21001 aaactacaac ggttagcgtt ccgccaacgc tatctttatt aaatttagcc attgtttgct
    21061 accctcgcta atctgcgttt gatattccct tgaactacta aatagtcaaa gattggagca
    21121 aataagttag caaataagat cgctaacatc ataccttcag ggtatgctgg gtttaccaca
    21181 cggattaata cacacataaa gccgattaag ataccgtatg cccatttacc tttgttggta
    21241 aatgcagctg ataccgggtc ggttgccata aagaacatac cgatcgcaaa accgcctaat
    21301 actaaatgcc aatgccaagg catagagaat aatgggttgg tatcagaacc gattacatta
    21361 aataatgtcg cagttgccgc cataccaatc attacaccgg caataattcg ccaagaagca
    21421 attcgggcaa atacaatgat tgcagcacca ataatcaaca ttaaggtaga ggtctcgccg
    21481 atagaacccg gaatattacc taagaacgca tccatccaag taatggtttc accggttgca
    21541 acgtgctgta atgcaccttg accaccaact gcccattgag aaagtgcagt tgcacctgag
    21601 aagccgtctg cggcaaccca tactgcatca cctgaaattt gacccgggta agcaaagaac
    21661 aagaaagcac gaccggctaa cgcagggttc ataaagtttt tacccacgcc accgaacact
    21721 tcttttgcga ccacaacacc aaaggtagtt gcaagggctg cttgccataa cggtaatgtt
    21781 ggaggaacaa ttaatgctag taagatagag gttacaaaga aaccttcgtt gatctcgtgc
    21841 ttacgcacca ttgcaaacac cacttcccag aaaccgccca ctgcaaatac tgttaagtag
    21901 attggtagga aataggttgc ccccagcaac attttagttc cccagcctgc atcagctaca
    21961 gagcctaaac tatcggctaa gctgtagtgc cagttgttag caactaaatc cgttaatgtt
    22021 cccattttga gtgccgctag aataccctgt gcaccaacat tgtacattcc gtagaacatc
    22081 gcagggaata acgctaacca tacaattaac atcatacgct tagagtcaat cgcatcacgc
    22141 acgtgagcat ctttacgagt gaccgtaccc ggtgtataga aaatcgtata ggcagcttca
    22201 aatagcgtgt accatttctc atactttcca cctttatgga aagcgggttc cattttttca
    22261 aaaagatttt tcaaacccat ttttaaccgt ccttctcgat cttatctaac gcttgacgta
    22321 ataatggtcc ccagttgttt ttaccggaac acacaaaagt acataacgct aaatcttctt
    22381 cgtctaactc taagcagcct aaagcttgag cggagtctgt atctccagct gctaaatcac
    22441 gcaataagag tgttggaatg atatccaacg gcattacacg ctcataagcc cctgttggta
    22501 ccattgcacg ctcactaccg tgaactgctg ttgtgaagtt aaataatttc ttcgcaaaat
    22561 gccctaaagt agtacgcgta attgaatact tatcagagta aggagcaatc ataccgaaga
    22621 acgctttctc acgcccttct aataaagcag acacttgtaa tgcgtaacga cctaaatagt
    22681 cgtgtactcc cgttgcgata gcaccactta acacagaacc tgaaatgaca cggttttcac
    22741 ccgcatttaa ttcgttggct gttaattgtg aaagattcgc ccctaaacga gtacgcacca
    22801 aacgtggatt tttcacttgt ggcccggcta atgcgactac acggtcagta aatagctcac
    22861 cggtcgtaaa taatttaccg atagcaatta catcttggta atttaaatac caaacttgtt
    22921 tagttgcacc taccgggtca ataaagtgaa tatgcgtacc agaaagacct gcagggtgcg
    22981 gacctttaaa gcggttagtt tccacattat tgatctgacg gctttgtgcc gcatttaaaa
    23041 tatttgagtc tgcactgcca cagagatgaa tcactttttt actttcaaat aaacgactta
    23101 atacagttaa gccatcaaag aaagcttgtt ggtgttctgc aataatcact tccggatttg
    23161 ctgcaagtgg attggtatcc atcgcattga caaaaataga ggaaggttct gcatctaatg
    23221 caggcacttt gctaaacgga cgtgtacgga aagccgtcca taagcctgac tctacaaggt
    23281 tttgttttac ttgctctgaa gttaatgaag ctaattggct ggcttcataa cgagcaaagg
    23341 taatttgctc atcaccctca actttaatta ctaccgattg aagaacacgc ttctcgccac
    23401 ggttaattgc cgttacggta ccacttgcag gggcagtaaa taccacgccc ggattctttt
    23461 tatcttcaaa aagaacctga cctttcttta ctacatcgcc ttcacgcact ttcattgaag
    23521 gacgcatacc cacatattct tcaccaagca tagcaacttc agtaacggta ttaccgttat
    23581 ggattacttg ctcaggtttt cccgcaatcg gaagatctaa gccttttttg attgtaatca
    23641 tattgtttgc actacttttc ttttgggtta aaaaatatga ttgcgctaac gcaatcgctt
    23701 ctattgagcc gcagctttac ttaaaaataa acaatggtcc cccacctatt tttaaaagcc
    23761 acattaagtt ctcaaatctg tgatcaacct ttat
//
LOCUS       DANLXQ010000020        22360 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.020, whole genome shotgun sequence.
ACCESSION   DANLXQ010000020 DANLXQ010000000
VERSION     DANLXQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22360
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.020"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..662
                     /locus_tag="PXD94_000627"
     CDS             <1..662
                     /locus_tag="PXD94_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6285519.1"
                     /translation="KEWFTASEVAGMGNLPEQPSNVTRRATKNNWLKRQIEGKRGVAF
                     EYHYSSLPISVQQELGFAQTAVKEEPRTLVAAQTADEMERVPFYNVQASAGFGAFNSE
                     VYAPDDYIGLSKRWLDVRGFYLSYLAFITASGDSMYPTISDGDMLLVNRATTQPKDGK
                     IYVFRQGEQLWVKRVQGIMGGIRLISDNKELYSPIDVIFDETLDFEVIGQVVYIGHEM
                     I"
     gene            710..3562
                     /locus_tag="PXD94_000628"
     CDS             710..3562
                     /locus_tag="PXD94_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6285520.1"
                     /translation="MVQAVSFSHFFAIHYFYFFLHKTKGQCTAKPLFSILLFHLFPKN
                     SAKFLLFFYFLVLMVLYSNEVANGKLLITGGTNLNGTLSAEGTSEIILSGRPTPHAYD
                     KVANKEVLIEGDWINRSFNATIFAAKDNGKLEISRNVANINGNFNLTDNATAQIGFTQ
                     GTSQACIRSDRTGVATCNINATLSETDLNSWERTKVAGNVSLADNSTFSLGSKADLTG
                     SITAQESTKVQLNDGSIANLTGLSTTGIFNGANGSTVNLSGVWTTLTNTVVDVLNVAN
                     GSQIHLNTPTATKQNTLTANTLTGNGIFKFVADLTKTASDIVTIKDTASGQFSIEIST
                     NGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEYRYHLLKEGNVYYLNPEKEEEPKIT
                     QKGPSETADPLPEYTGDIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNG
                     NAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQKQNTLTANTLTGNGIFKFVADLTKT
                     ASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVHSGEYRYH
                     LLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPEYTG
                     DIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAV
                     LGVTTALDHVIHSEGTQNWKVWSKTDYQTTEHRSAGYGFNQDANVIHLGLEKALDNQV
                     TLGMILSQSKARNNFDHYYSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQ
                     ANTVKLDRSVFVTGLSIGKAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDL
                     LALQAGLSINKTLELNGLQIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAG
                     ISANYRNINAGIQAGFLTGNTLNKQRYISFKVTYEW"
     gene            complement(3637..4473)
                     /locus_tag="PXD94_000629"
     CDS             complement(3637..4473)
                     /locus_tag="PXD94_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL6285521.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERMPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAERHDYRYSDEELK
                     GLAELLFSRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
     gene            4581..5456
                     /locus_tag="PXD94_000630"
     CDS             4581..5456
                     /locus_tag="PXD94_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6285522.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLWVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            5592..6032
                     /gene="nudB"
                     /locus_tag="PXD94_000631"
     CDS             5592..6032
                     /gene="nudB"
                     /locus_tag="PXD94_000631"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL6285523.1"
                     /translation="MNYKNPHSILVVIYAKNTQRVLMLQRKDDPDFWQSVTGSINIGE
                     TPLEAAEREVSEETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLL
                     PLDSEQEPILTEHLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            6045..6785
                     /locus_tag="PXD94_000632"
     CDS             6045..6785
                     /locus_tag="PXD94_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6285524.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
     gene            6915..7442
                     /locus_tag="PXD94_000633"
     CDS             6915..7442
                     /locus_tag="PXD94_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285525.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKVAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     KLCDVDKALTSKLNK"
     gene            7509..8087
                     /gene="ruvC"
                     /locus_tag="PXD94_000634"
     CDS             7509..8087
                     /gene="ruvC"
                     /locus_tag="PXD94_000634"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL6285526.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(8153..9433)
                     /gene="murA"
                     /locus_tag="PXD94_000635"
     CDS             complement(8153..9433)
                     /gene="murA"
                     /locus_tag="PXD94_000635"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL6285527.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNIAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(9543..9803)
                     /locus_tag="PXD94_000636"
     CDS             complement(9543..9803)
                     /locus_tag="PXD94_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL6285528.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            complement(9836..10159)
                     /locus_tag="PXD94_000637"
     CDS             complement(9836..10159)
                     /locus_tag="PXD94_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL6285529.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            complement(10171..10806)
                     /gene="mlaC"
                     /locus_tag="PXD94_000638"
     CDS             complement(10171..10806)
                     /gene="mlaC"
                     /locus_tag="PXD94_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL6285530.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            complement(10852..11376)
                     /gene="mlaD"
                     /locus_tag="PXD94_000639"
     CDS             complement(10852..11376)
                     /gene="mlaD"
                     /locus_tag="PXD94_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL6285531.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            complement(11397..12173)
                     /gene="mlaE"
                     /locus_tag="PXD94_000640"
     CDS             complement(11397..12173)
                     /gene="mlaE"
                     /locus_tag="PXD94_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL6285532.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            complement(12166..12969)
                     /gene="mlaF"
                     /locus_tag="PXD94_000641"
     CDS             complement(12166..12969)
                     /gene="mlaF"
                     /locus_tag="PXD94_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL6285533.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGPSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIREHTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            13194..14588
                     /locus_tag="PXD94_000642"
     CDS             13194..14588
                     /locus_tag="PXD94_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL6285534.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            complement(14640..15401)
                     /locus_tag="PXD94_000643"
     CDS             complement(14640..15401)
                     /locus_tag="PXD94_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL6285535.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(15388..15840)
                     /gene="nrfF"
                     /locus_tag="PXD94_000644"
     CDS             complement(15388..15840)
                     /gene="nrfF"
                     /locus_tag="PXD94_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL6285536.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTRKNKGNKNENA"
     gene            complement(15830..16363)
                     /locus_tag="PXD94_000645"
     CDS             complement(15830..16363)
                     /locus_tag="PXD94_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL6285537.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWVSWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            complement(16360..18264)
                     /gene="nrfE"
                     /locus_tag="PXD94_000646"
     CDS             complement(16360..18264)
                     /gene="nrfE"
                     /locus_tag="PXD94_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL6285538.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            complement(18534..19481)
                     /gene="nrfD"
                     /locus_tag="PXD94_000647"
     CDS             complement(18534..19481)
                     /gene="nrfD"
                     /locus_tag="PXD94_000647"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL6285539.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTHALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            complement(19481..20158)
                     /gene="nrfC"
                     /locus_tag="PXD94_000648"
     CDS             complement(19481..20158)
                     /gene="nrfC"
                     /locus_tag="PXD94_000648"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL6285540.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIIDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            complement(20158..20814)
                     /gene="nrfB"
                     /locus_tag="PXD94_000649"
     CDS             complement(20158..20814)
                     /gene="nrfB"
                     /locus_tag="PXD94_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL6285541.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCISC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAHDT
                     HATKIACTNCHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATEAKGTK
                     "
     gene            complement(20833..22335)
                     /gene="nrfA"
                     /locus_tag="PXD94_000650"
     CDS             complement(20833..22335)
                     /gene="nrfA"
                     /locus_tag="PXD94_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL6285542.1"
                     /translation="MKFLIKSLAVATISILGCLQTALAEEAKTESLTDKAVKHEKLGV
                     KIESANHLFAEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGH
                     FYAVTDVRDILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGA
                     EIVNSIGCADCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCA
                     NCHVEYYFKNKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEV
                     WSQGTHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEK
                     RVKEYKHEVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSA
                     AGHGNHFHAPDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLA
                     IGLDIPKEQAAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
BASE COUNT         6720 a         4843 c         4522 g         6275 t
ORIGIN      
        1 gaaaggaatg gtttacggca tctgaagtag ctggaatggg aaatttacct gaacaaccat
       61 caaatgtaac aagaagagct accaaaaata attggctcaa aagacaaata gagggaaaaa
      121 ggggggttgc ttttgaatac cactacagct ctctcccaat aagcgtgcag caagagttag
      181 gctttgctca aacggcagta aaagaagagc ctagaacatt agtggcagcc caaactgcag
      241 atgaaatgga acgtgtgccg ttttataatg tacaggcttc agcaggcttt ggtgctttca
      301 atagcgaggt gtatgcccct gatgactata ttgggctaag taaacgatgg ctagatgtgc
      361 gtggcttcta tttgagctat cttgcattta ttaccgcttc aggtgattca atgtacccaa
      421 ccatcagtga tggcgatatg ctattggtaa accgagccac cacccagcct aaagacggta
      481 aaatttatgt gttccgccaa ggcgagcagc tttgggttaa acgtgtgcaa ggcattatgg
      541 gcggtattcg cttaatcagc gataataaag agctatacag ccctattgat gtgatttttg
      601 atgaaacgtt agattttgag gttatcgggc aggttgtgta cattggacac gagatgattt
      661 aaacgccatt taaagctact taaatcattt ttactttttc gttgttttaa tggtacaagc
      721 ggttagtttt agccattttt tcgcaattca ttatttttac ttttttctac ataaaacaaa
      781 ggggcaatgc accgcaaagc ccctgttttc aatacttctg tttcacttat tcccgaaaaa
      841 ttccgccaaa ttcctattat tcttttattt cttagtttta atggttctat acagtaatga
      901 agtagcaaac ggcaaattgc ttatcactgg gggaacgaac ctcaacggta cgctttctgc
      961 agaaggtaca agcgaaatca ttttatcggg ccgtccaaca cctcatgcct atgataaagt
     1021 agcgaataaa gaagtcctga ttgaaggcga ctggatcaac cgatcgttca acgcaacaat
     1081 ctttgcagct aaagacaatg gcaagcttga aatcagccgt aatgttgcca atattaacgg
     1141 taattttaat ttaacagata atgctaccgc tcaaattggt tttacccaag gtacctcaca
     1201 agcatgtatt cgctctgacc gcacaggcgt agcaacctgt aatattaacg ctactttatc
     1261 agagactgat ttaaacagct gggagagaac caaagtggct ggcaatgtct ctttagctga
     1321 caattcaaca ttttcgttag gcagtaaagc agacttaact ggctcaatta cagctcagga
     1381 gtccactaag gttcaactta atgatggaag tattgctaat ttaaccggct tgagtacaac
     1441 aggaatattt aatggcgcca atggttcaac cgttaatctt tctggggtat ggacaacctt
     1501 aaccaacact gttgtagatg tcttgaatgt cgcaaatggt agccaaatcc atttaaacac
     1561 tccaactgca acaaaacaga atacgcttac cgctaatacc ctcacaggga acggcatctt
     1621 taaatttgtc gctgatctga cgaaaacagc gagcgatata gtcacaatta aagacaccgc
     1681 aagcggtcaa ttttcgatag aaattagcac aaatggtagc gcccattcag cccaaaagat
     1741 taaattattt gaaacaacaa atgaagaaga cttttctctt aatttagcaa atgaagtgcg
     1801 ctcaggcgaa tatcgctacc atttactcaa agaagggaat gtgtattacc tcaatccaga
     1861 gaaagaggaa gaacctaaaa ttactcaaaa agggccaagt gaaaccgctg acccattacc
     1921 tgaatacacc ggggatattg agcattctga aaaagggccg ggtgaaactg ctgatccgtt
     1981 acctgaatac acaggcgaca ttgagcattc agaaaaagga tcgggtgaaa ccgctgagcc
     2041 attacctaaa tacaatggaa atgcacattt gatcgcatcc gagttaactg cgcaaactaa
     2101 tgctgtatta ggtgtaacta cagtattaga tcatgtaatt cattcggaag ggacccaaaa
     2161 acagaatacg cttaccgcta ataccctcac agggaacggc atatttaaat ttgtcgctga
     2221 tctgacgaaa acagcgagcg atatagtcac aattaaagac accgcaagcg gtcaattttc
     2281 gatagaaatt agcacaaatg gtagcgccca ttcagcccaa aagattaaat tatttgaaac
     2341 aacaaatgaa gaagactttt ctcttaattt agcaaatgaa gtgcactcag gcgaatatcg
     2401 ctaccattta ctcaaagaag ggaatgtgta ttacctcaat ccagagaaag aggaagaacc
     2461 taaaattact caaaaagggc caagtgaaac cgctgaccca ttacctgaat acaccgggga
     2521 tattgagtat tctgaaaaag ggccgggtga aactgctgat ccgttacctg aatacacagg
     2581 agacattgag cattcggaaa aagggccggg tgaaactgct gatccgttac ctgaatacac
     2641 aggagacatt gagcattcag aaaaaggatc gggtgaaacc gctgagccat tacctaaata
     2701 caatggaaat gcacatttga tcgcatccga gttaactgcg caaactaatg ctgtattagg
     2761 tgtaactaca gcattagatc atgtaattca ttcggaaggg acccaaaatt ggaaagtatg
     2821 gtctaaaaca gattatcaaa cgacagagca tcgctctgcc ggatatggat ttaaccaaga
     2881 tgcgaacgtt atccatctag gattagagaa agccttagat aatcaggtca cacttggtat
     2941 gatattatct caatcaaaag cgagaaataa ttttgaccat tattatagcg gtaaaggtcg
     3001 cttaacaatg ctatcaatgt atgcgaagaa aacatggcaa aatggtgttt ttgttgcgat
     3061 agataccggt tttgggaaag caagcaatcg cttaacttat caagcaaata cagtaaaatt
     3121 agatcgttca gtatttgtaa cagggcttag tatcggtaaa gcatgggaat ctgcaaacgt
     3181 gaatataatc ccatcattta gcgcccgtta tcatcattta tcttcagcag gtaatcaact
     3241 tgtagatgca aaaatcgaaa caaacgctgt tgatcttctc gctcttcaag ctggtctgtc
     3301 aattaataaa acgcttgaac taaatggcct gcaaataaaa ccggaaatag gtagctattt
     3361 tgttgatgca agccatggga aattaagaac acgtttcaat aatttacaaa tagagcagca
     3421 aatggggcgt tattttaaac aagaagcagg tatatccgct aattatcgca atataaacgc
     3481 aggaattcag gcaggtttct taacggggaa tacgctaaat aaacagcgtt atatctcgtt
     3541 caaagttaca tatgaatggt aaatattgag attaagggct atccattact aaatggcatg
     3601 gccctttatt tactcctcaa caatgtaaat tacccatcaa aacaaactct gctgcccaag
     3661 caaattctcc ggcacaggtt tgatttgaaa tcctttttcc gccaaaaagc cttttagggt
     3721 ttgaatatta taaaaggcgt ggcttttagt ggtgttttca aagtagagat agagcctgtc
     3781 aaattccgtt ctgcggctaa aaagcaattc tgccaagcct tttaattcct cgtctgaata
     3841 gcggtaatcg tggcgttctg ccgcactctg ccctttccac caattcgggt tacgaccatg
     3901 taagcgtaaa taagcagtac gttggttggc aaaaaagtga aatgccggta agccgatatt
     3961 ttgtgggtaa tccacattac accaaatcag attagagctt ttcgcaaagg tatcaaacac
     4021 cgccggaata tgccagcttg ggtggcgaaa ttcaatcgct aacggataat ccgcaaacca
     4081 ttggcacaac tcggcaaggt aacggcggtt aagtggcata cgttcaaatt gagttgggaa
     4141 ctgaacaaat aaatttgcca ataacccttc agcggtgaac ggagctaacg cattaagaaa
     4201 taattgtgct tgatctaaag tcgccgtgcg ttgatggctg aaatcttgat gcagtttcac
     4261 cgaaaaattc agccgacctt ccgctttctg caccattcct tcaaaggctt tctgcccgat
     4321 gggggcgtgg aatgtgctgt taatttccag cgtgtcgtaa tgttggctat agacaaagag
     4381 aaaatcctct tttgcagtgc cgaatgggta aagcgtgccg attaaatctg tgtcggcata
     4441 gccgccggtg ccaatataaa tatgagggag cattgtgttt attctttcaa ttttttgcaa
     4501 attttaatgg ttttttaacc gcttgcaaac tgagaataaa agagtaagat ttggctatct
     4561 tttcttattt catcatcatt atgtggattt ggtttactct gctggcagcc gtggctcaag
     4621 ccggtcgcaa tgcctttcaa aagcaattaa gcgtcaatgt gcctgtattg ggggtaacgc
     4681 tcgcccgctt tttctatgct gtgccgttgg caagcggtta tctctatttt ttataccaat
     4741 atgacgataa cctcaacatt ccccattttc caccgctgtt ttttgtatat gctatcgggg
     4801 ctgggttggc tcaaattctg gcaacttcgc taatggtgca actttttcac ttaaaaaatt
     4861 atgcaatagg tgtcgggctg gcgaaaagcg aggcagtatg ggcggcttta ctgggcgtgt
     4921 tattttttgg tgtaaccatc agtggcattg gctggcttgg cgtggtacta ggtggagtgg
     4981 cgattttctt aatgaccggc agcggtaatc tacgccaact ttcgtggaaa acactctttc
     5041 tcggcatagc cagcggttta tgtttcgccc tcacatcact ttgggtgcga gaggcaagcc
     5101 tgcaactcaa cagccactat ttgctttcag cggcgtgggt actttttagc gtgattagct
     5161 ttgaggcagt gatattggtt atatacctag ctcttctcca aaaagagaca ctgattaaat
     5221 tctttcgttt tccaaaatta ggtatgttga ccagcctgtt ttcatttctc ggctcattcg
     5281 gttggttcaa tgcaatgagc ctgaaccacg ttgcctttgt aaaaacgctt ggacaagtgg
     5341 aaattttctt tattctcggt atttcctatt ttgtgtttaa agaacggctt aaaattcaag
     5401 attttatagg gctgatttta gtagtgattg ccgcaatctt ggtggtgctg ggctagatcg
     5461 gatttttacg ttttttatct agctttgtag ggtgggcgta agcccacgca aaaccaataa
     5521 atcatttatc cgtgggctta cgcccaccct acgataccta aaaaaattaa atttcacatc
     5581 ttagtaataa aatgaactat aaaaatcctc atagtatcct tgtggtgatt tatgctaaaa
     5641 atacccaacg agtattgatg ctacaacgca aggacgatcc tgatttttgg caatcggtga
     5701 ccggttcgat caatattgga gaaacaccgc tggaagcagc agaacgggaa gtgagcgaag
     5761 aaaccggttt gcaaactttt gagcaaaaac aaccgcttgt tgattgccaa aagcagatag
     5821 aatttgaaat tttcccgcat tttcggtata aatacgcacc taatatcaca cattgtgtcg
     5881 aacattggtt tttgctgcca cttgatagtg agcaagagcc tattttaacc gaacatttgg
     5941 catatcgttg ggtttcagtg gaagatgcgg ttcgactgac taaatctccc aacaatgccc
     6001 aagcaataca agaatattta acatcaaaat aaggaaacaa cagaatggca ggccatagta
     6061 agtgggcaaa tattaaacac cgtaaagcgg cacaagatgc acaacgcggt aaaattttta
     6121 ctaaattaat tcgtgaattg gtgactgccg ccaaactcgg cggtggtgat acctcttcca
     6181 accctcgttt gcgtgcggcg gtggataaag cgttatccag caatatgacc cgtgatacca
     6241 tcaaccgtgc aatcgagcgt ggtgtaggcg gtggcgatga taccaatatg gaaaccaaaa
     6301 tctacgaagg ttacggcccg ggtggtacag cagtgatggt ggaatgttta agtgataacg
     6361 ctaaccgtac gatttcccaa gttcgtccaa gtttcacaaa atgtggcggt aacctaggta
     6421 ctgagggttc ggtaggttat ttatttagca aaaaaggctt aattttagtg gcaaatgccg
     6481 atgaagatgc cttaactgaa gctgctattg aggccggtgc ggacgatatt caaccacaag
     6541 aagatggtag ctttgaaatt tataccgctt gggaagattt aggctcagtg aaagacggca
     6601 ttgaggctgc aggctttaaa attgagaatg cagaagtaac aatgattcca tcaactaccg
     6661 ttgaacttga cgcagaaact gcaccaaaac ttcttgattt aatcaaccgc cttgaagatt
     6721 gtgatgatgt acaaaacgtt tatcacaatg gtgaaatcag tgacgaagtc gctgcattac
     6781 tgtaataaaa aaatcacgca aaataagcat aatttaggtt atgcttattt tttatgaact
     6841 tttttcctat attactgact aacctaatgt agtttatatg ctactacttt actataacat
     6901 tttaaggaga gtctatgaaa ttagtgaaat ttgtccctgc cctagcagca actttagtat
     6961 tagccgcttg ctctaatgca aacacacaat ctgctcctca gcaagctcag gacgctgtga
     7021 ctaaagtggc tgaacaagca acacagcaag tgaatgaaat tgcaggtaat gcacctcgtg
     7081 caagtggtgc agtaaaagta gaaaaagcaa ctgatggtgc aaaatcaatt gtatatcgtt
     7141 gccaaagtgg taaacgtgta actgcgaatt atgtcttcca aggagaagag gcaaaagcag
     7201 taaatctatt agtccaaagc ggcggtaaaa cacaaaagat cccaacatta actcgtgatg
     7261 cctcaaacca agattctact tcattcaaaa cggataaata ttcttgggat ttagacacag
     7321 gctttacctt atcatctaat ggccagtcag gtatgctaac caagtatggt gctaaaacag
     7381 ataccatcgt tgctaaatta tgtgatgtag ataaagcatt aacctcaaaa ttaaataaat
     7441 aatctttctt gattttacta ctcataagcg agaagataat atcttctcgc ttattttatt
     7501 gcacaaagat ggctattatt ttaggaattg accccggctc aagggtaaca ggttacggtg
     7561 tgattcgcca aaacgggcga cagctggaat atttaggaag tggggctatt cgaaccacgg
     7621 ctgaagatct gcctacccgc ttaaaacgga tttatgcagg cgtgagtgaa atcatcactc
     7681 aatttcaacc aaatatgttc gccatagagc aggtatttat ggcaaaaaac cctgattctg
     7741 ccttaaagct cgggcaggct cgaggaacag cgattgtagc agcggttaat cacgatttac
     7801 ctgtgtttga atatgccgcc cgattagtca aacaaaccgt tacgggaatt ggttctgctg
     7861 ataaagtaca agtgcaagat atggtaaccc gtatgcttaa actctccgcc aaaccacaag
     7921 ccgatgcggc tgatgcatta gccattgcga ttacccacgc tcattcaatc caacattcac
     7981 tgattgttgc taagcaaagc cagcagaatg tgagtagtga gaaagagcaa attttagcac
     8041 taatgaaaac ccgttatagc cggggaaggt ttaggttaaa aggctaactc aatataaaat
     8101 gaccgcttgg aactaatgtt tcaagcggtc attttttgag aaaattttac aattactcgc
     8161 cttcaaaagg agctgagaaa cgttcaattc tcgccccaat ctgacgtaat ttttcttcga
     8221 tgtgttcata gccacggtct atgtggtaaa ttctatccac aatcgtttca cccgttgcaa
     8281 tacagcctgc caacactaag ctgatggaag cacgcaagtc agttgccatt acttctgctg
     8341 attttaactg ctctacacca tagcaaattg caatattacc ttcaatttcc gctttggcac
     8401 ccatacgaat cagctcagga atatgcataa aacggttttc aaaaatcgtt tctgtaatac
     8461 ggcttgtgcc ttctgcaact gtatttaata aggtaaattg agcttgcata tcagttggga
     8521 aacctgggtg tggcatagtg cgaatgttca ctgctttcgg gcgacgacct agagaatcaa
     8581 gcgtgatagt attttccgtc acatcaacct gcattccagc ttcacgtaat ttttcgatca
     8641 ctgcatcaag agtgtctgct ttggtgttac ggcaagtaat tcgaccaccg gaaatagccg
     8701 ctgccactaa aaatgtaccg gtttcaatac gatccggcac tacgctgtgt tcgcaaccgc
     8761 ctaaatgctc aaccccttca atggtaatca tatcggtacc tgcaccagaa attttagctc
     8821 ccatcgcatt taagaaatcg gcagtatcta caatttcagg ctcacgagca gcattttcaa
     8881 taacagtagt accttttgct agagtacctg ccatcatcac cgataaagtt gctcctacgc
     8941 tcactttatc catatagatt cttgcaccgt ttaaacgacc gtttgaagtt gctttgacat
     9001 agccttcatc aagctcgatt ttcgccccca ttttttcaag accggaaatg tgcatatcta
     9061 ccggtcttgc cccgatagta caaccgcccg gtaaagaaac ttgcccaagt tggaaccggg
     9121 caactaatgg agctaacgcc caaattgaag cacgcattgt tttcactaat tcgtaaggtg
     9181 caacgtaatt attgatctta ctcgcatcaa tatgtaccac gcctttatcg ctaccttttt
     9241 caaccgttac ccctaattgg cgtaagattt taaaagtggt gtccacatct tttaaatcag
     9301 gcacattttt taacgtaact gttttttcag ccaaaatagc ggcaaataaa atagggagtg
     9361 cggcattttt cgcacctgaa atatcgacag taccatttaa agaaaatgga ccatacacac
     9421 gaaatttttc catttgggag atcctaaaaa ataataattt gtaggggcga aaaatttttc
     9481 gcccaatttt ccaacgttta caaaagagtt tgggcgaata gtgattcgcc cctacatttc
     9541 atctatccaa ccatatttag caaacgctca gttttccatt tttccacgct gaatacttta
     9601 atggttaaag cgtgaatcgc attagtagtg aaatactcag ctaaaggggc ataaacagct
     9661 tgctgttgtt ttacttttga aagagaagca atgctgtcac ttactacaat aacgccaaag
     9721 tgggcattct cgccttgagc gtggacttct gttgcatcag ataatgcttg ttttaaaatt
     9781 tcttcaattt gtgatggatt cattctgact tcctaaaata tgattggtaa ctcactcaaa
     9841 taataaattg ctcaaaccaa ccgtctaaat cgtataatgc agccaaagtt ctgacagaat
     9901 ctggcgtatt aatcaaactg tttggtatga ttttatggta atgattcagt aattcagcaa
     9961 gtaaggtaaa accggcagaa tcaatccgag tgatttcttt taaatcccaa tataggcttt
    10021 gtttccctgt tggagataaa aaagaagcac gttgcttcca taaagggagc aacgtgttac
    10081 gagttaattc gcctgatagt ttcacaaaaa gactttcgtt attttgctga atgtcccatt
    10141 gtaatgtttt ttgaggcata actaaactac ttattttaaa gtgataggct gttttgcaga
    10201 ttgtgccact tgtgcaatta aggcatcaat ccctttttga cgaatcacac ccgcccattc
    10261 gttttgcttg gttgcaacca tacttactcc ttcagcggcc atatcgtatg cttgccattc
    10321 acctgttttg ctgtttttac gccatttaaa atctaatttg atcggctgtg cagcaccttt
    10381 acctgacacg tttacacgaa tgcttacgat agatttacca tccaccgatt tcgggctttc
    10441 aatttgcaca ctttgatctt gatactgggt taacacctga gcataagact gctcaacaaa
    10501 ttgaccaaaa gcattaaaga atgcctcttt ttgtgcatca gtcgcagccg ataagttttt
    10561 ccctaacact aattggcctg catatttcac gtgaacgtgt ggcattaaat cgttacgcac
    10621 aatggtacgc aaatattcag ggtttgattt aattttcgct tgatttgctt taatatcgcc
    10681 aaataattta tctgcggttt gttgcattaa tacataaggg ctagtttcag caaatgcggt
    10741 ggttgaaaat aatgcagaaa ctgccactaa gcctgttacg ataatttttt taatgttttt
    10801 taacattatt gatgctctcc aaaactctaa attagaggtt gttgtactta attactctgc
    10861 ttttggttca gcagaatcac ttgctttttc atcttcagtt ttatctgact ttttatcacc
    10921 atataagaac tgaccgatta aatcttctaa caccattgca gaattggtat ctacaaaagt
    10981 atcaccttct ttcatcattg cagtttcacc ttcgattacg aagccaacat ttaacgcaac
    11041 gtattgctcg cctaataagc ctgaggtttt aattgagagc gagctggtat cgggaatttg
    11101 attaaaactt tcatttaccg ctaatgccac tttcggggtg taggttttgt tatcaagtga
    11161 aatatcagcc actcggccaa ctaccacacc gccaacttta ataggcgcac gaacctttag
    11221 tccaccaata ttatcaaaag ttgcatacag ggtgtaagtt ttctcatttg agaagccctg
    11281 cacatttgct actcttaagc ctaaaaagac aagagctgca agcccaagta gtacaaataa
    11341 gcctacccaa aattcatatt taattgattg acgcattgtc tgttcctttc aaaaaatcaa
    11401 ccgccgaaca ttatcgcagt taaaataaaa tccaatccta aaatcaccag tgaagcattt
    11461 accaccgttc ttgtggtcgc ttggctgatt ccttctgaag tcggtacgca atcgtagccg
    11521 ttaaataagg caatccagac caccgcaaag gcaaaaatca cacttttaat aaagccgtta
    11581 attaaatcgc tggtatttac cgagttttgc attaccgacc aaaaactgcc accatcaacg
    11641 cctttccaat ctaccccgac taatgagccg ccccaaattc cgatagcggt aaaaatagca
    11701 gctaacaccg gcattgaaat cacgcctgcc caaaagcgag gagcgatgat acgtctaagc
    11761 ggatcaaccg ccatcatttc aagactggaa agttgctctg tggctttcat cagcccgatt
    11821 tctgcggtca atgccgaacc ggctcgccct gcaaataata atgcagtaac tacaggacca
    11881 agttctcgta aaagcgagag tgaaaccaaa gttcccaagc tactttcggc ggcaaaatcg
    11941 accaatacca catagccttg taagcctaac accattccga taaataagcc cgacagcata
    12001 ataattaaga gcgattgcac tcctaacaca taaagctgtt taatcagtaa cggggtatgt
    12061 tttctaaatt caggtttacc gattaatgct ccccataaca taaaagcgga acgccctaat
    12121 gtacggataa aattaatcac caccgccccg atagagctaa taaaattaac cattaaatag
    12181 ctcctccgta taatcttttg ctggataatg gaaccgcacc ggaccatcag ctttaccggc
    12241 taagaattgt accacttgcg gatcttcgct tgcctgtaaa atttccggtg taccttcagc
    12301 aatcactcgt ttatctgcca caatataagc ataatcggca atacttaata cttctttcac
    12361 atcgtgagac accacaatgg aggtcaaatt caatgcctga tttaactctt taatcagctc
    12421 gacaatcaca cccatactaa tcggatcttg cccggtaaac ggctcatcat acataatcag
    12481 atccggatct aacgcaatcg ctcgagcaag ggcggcacgt cttgccatac cgccggaaag
    12541 ttcggacggc attaaatttg ccgcacctcg caacccaacc gcctcgagct tcaataacac
    12601 caatttgcga attagctctt cgggtaaacg ggtatgctcc cgaatcggga aagccacatt
    12661 atcgaaggta gaaatatcgg taaataacgc ccccgattga aacaacatcc ccattcgttg
    12721 gcgaatttca tacaactctt tgttattggc tttgcaaata tctctgccat caaataaaat
    12781 ttcgcccgat tcaggtaaaa tctgcccacc gattagcctc aacagggtgg ttttaccaat
    12841 cccagaaggc cccataatgg cagtcacttt acctttctgc acttgcagat tcaggttatc
    12901 gtaaatcact cgctcccctc gcttgaaggt caagtttttt acttcaacca aattttccgt
    12961 ttttgtcatc aaataggact caaattaata aaagtgatat ttatggtcta tcaaatttgc
    13021 cagaatgtca aaaaactttt aataatctga caaaaatctg cctttacaag cggtcagatt
    13081 tgctaaattt tttgcaaacc ggctgaactg aaaaagaaag gtcttgtctt agccaatacg
    13141 agcgttttca ctcgtagatt ataaaaacat aggatatgag gaacattcac aatatgaaca
    13201 aaacaatgaa aaaatcattt ttaactgcat tcattttagg ctcaaccatt gccgcaatgc
    13261 cggcgtatgc agaagcaaca ttacccacag ccgtaaatgg gcaagctgtg ccaagccttg
    13321 caccaatgct ggaaaaagtt cgcccggcgg tggtgagtat tgcagtagaa ggtaaaacca
    13381 aaggcgatag ccgccgcagt cgtgaaattc cggaagaatt tgaatttttc ttcggtcctc
    13441 acgccgattt attcggcaac cgctcatcag ctccacgcaa tttccgtggc gttggatcag
    13501 gtgcagtcat caatgccgaa aaaggttatg tgattaccaa taaccacgtg atcgacaatg
    13561 ccgataaaat taccgtaaaa cttgaagacg gacgtgaatt taaagctaaa ttagttggtg
    13621 ctgatcccct ttcagatgtg gcgttaattc aagttgaaga tccaaaaaac ttaactgaaa
    13681 ttaaattcgc cgattccgat aaattacaag tgggcgattt tacagttgca atcggtaacc
    13741 cgtttggctt aggacaaact gttacttcag gcatcatctc ggcacttggt cgttcaaccg
    13801 gtcaatccga tgaaggctat gaaaactata ttcaaaccga tgcggcagtt aaccaaggta
    13861 actctggcgg cccgttaatt aacttaaacg gcgaattaat cggaattaac accgctatta
    13921 tttctccaag tggtggcaat gccggtatcg cctttgcgat tccaagtaat atggcaaaca
    13981 gcttggtgac tcaaattatt gagtttggtg aagtaaaacg tggaatgtta ggcattaaag
    14041 gcggtgaatt gaatgctgac cttgctaaag agttcaatat ttccacccaa caaggggcat
    14101 ttgtaagcga agtcttaccg gattctgctg ctgctaaagc cggctttaaa gcaggtgatg
    14161 tgattactga aatcaacggt caaaaaatcc gtagtttcag cgaattacga gcaaaagtag
    14221 ctacttcagg tgtaggtaaa gagattgaac ttacttacct gcgtgacggc aaagaagcca
    14281 aagcaaaagt gaccttacaa tcggattctg aagccaaagt tactgcttca aacctcattc
    14341 cggcattaaa aggggcagat tttaacaact acaatgccaa aggcattaaa ggggttgaaa
    14401 tttctaacgt agaaaaaggc tcgattgctg aaatgcgaag cctcaaaaaa ggcgatatta
    14461 tcatcggcgt gaaccgccac tcggtagaaa acattgccga tttacgcaaa atattagaaa
    14521 ccaagccatc tgtggttgca ctcaatattg tgcgtgacgg tgcaaatttc tatgtgattt
    14581 tgcaataaac cttttgatcc actcaaacaa aagccgatga caaaaatcat cggcttttgt
    14641 tattgctggg aatttaacat ctgtcgtgcc atttccatac tttggataat cgctctgcgg
    14701 ttgatagaat cattattgct atctaacact ttacgccaat tatcgagagc ttgttgaaaa
    14761 ttgttattta aaaagctgtc ggaggctagc aggagtaaac tggcattttc atttttatcc
    14821 aactgtaggg cttgctcgat aatggcttga atttcaacgg tcattttctg cccgttatcg
    14881 taataacgag cggtggcaat cgcccccaaa atagccgctc gttttccgag tagcttctcg
    14941 gcattatcgt agcaaaccag tgccgaatca aaatcattgc ttaacgcata agcctgccct
    15001 agttcgtacc ataaatcacc gttattcggg ttttctcgaa gctggttttg cagactcaaa
    15061 atatagcgtt catttttcga tgtgccgtgt gcatcgctca tctgttgttg aaaatcttga
    15121 tgttgttgca aacctgattg caccacttga taacgtccgg aaagcccata gtaagtcatt
    15181 gctaacaaca aaatcgccac gcaactcagg cttgcgattt tagcaagcgg tcgattttga
    15241 gcattatttt gcaaaacgct ctgttcaaat tgcgttcgtt caaattggta acgttgctct
    15301 gcctcacgct caaactcttg tctgacgtca tctgcaaaat gttgggcaaa tttaaccgct
    15361 tgttggtagt tttcttggct taattgctca cgcgttttca tttttgttgc ctttattttt
    15421 gcgagttcgc cacaaaatag ctagaaataa caggctaaac aagccaaccg gcaagcccca
    15481 taatacccat gtctgtggcg tgaatggtgg atcgtaattc acaaaactgc cgaaacgctc
    15541 ggtcataatg cggataattt cctcattact tttcccttga ttcaccattt catacacttc
    15601 aattcgcata ttgtaagccg ccgtcgcatt ggattcgacc aaattttggt tctgacattg
    15661 caaacatcgc aacgaacgag ccagtgaaat cgcccgtacc cgatccgcct cattttgaaa
    15721 ttgaaaggtg tccaccattt cagcttgaac cgtgccgaca aatagcaaca agccaaatac
    15781 aagcggtaat attttgcgga ttttttgcaa aaagccgatg gaaattatgc tatttctcat
    15841 ttctgagagc ctctaatttg ggtaggaaat cctgtgcaaa atcagggttg taaccctttt
    15901 gttggtaacg cactacaccg tgtttatcaa tcaaataact ggaaggtgca gaagagactt
    15961 tcagggtttg gatcaaaaag cctttttcgt aatcatcaat aattaaacta aacggatttc
    16021 cccagttttt caatgcgttg agggcatcat ttgggcggtc gctataagtt aagccgacaa
    16081 tcggcacgcc tttttgtttt agttccaaca aaatcgggaa ctctttaatg caccaagtac
    16141 accagctcac ccacacattg agaatataag gctctttcgg caagctgttg ttatttaaat
    16201 gcgcgttact atcaagtaag ttcttgccta caaactctgg aaacggcttg ccctgccaat
    16261 cttcagtggg agcaagggca tctttattca ttaacggcac agtcaaaaag ccaacaataa
    16321 ctacaagtaa caggagcgga ataagtaaca ggattttttt cattttttgt tttctctacg
    16381 ataaccgaat aatgccaaca gggcggccat taccatcatc ataccaccta accataaggc
    16441 ttgcacataa ggcttatagt gcaaacggaa ggcatattct aaattgccga acttatcccc
    16501 catcacaata taaatatcgt ctaaattgtg gtggaaaagc cctacttctg ccattgtcat
    16561 cgttcttaca tcataatagc gtctttccgg tgtgacggtt gccagcaact gttcatctcg
    16621 gctgatagaa aaaacggctt gctcggcggt gtagttcgga ccgatgagat cgtgataatc
    16681 ttcatattta aaggtataac cggcaagttc cgctgcttga ttcggcttta accgcacgcc
    16741 gatttcatcg ccgtaataac tgttcatcat tgccccaatc acgcaaaccg caaagccgat
    16801 atgggctaac cgcatcgcaa gcggtcgaat ttgccacatt ttttgcgaat agggcaaatg
    16861 agtgagaata atccagatcg caaggctgat aaatatggtg ggtaacggct gcaaagcaaa
    16921 atgctctctt tgcgagatgg aggcataaat caaccctaac gctaaaccga ctgcaatcgg
    16981 cagcaaccaa agttttacca atatctgctt atttgaaatc tgtttccagc ccagcaccac
    17041 cgcaaagccc attactatca ttaacagtaa cgctagtggc gtgaatacgc tattgaaata
    17101 gggggccccc actgaaatcg atccccaatt catcactgtg aagatcatcg ggtagaaagt
    17161 ccccagcaat accacagaag cagccacact aaataagccg ttaatcaata aaaatgccgt
    17221 ttctttagaa agtaagttga aacgcacttt gccttgccag agattcgctc ggaatgcaaa
    17281 cagagcaagg gcaatgaaac ttaagccgaa gaataagatc agcagtgcca ttcctctgtc
    17341 gggatcgacc gcaaaggcat gaaccgaggt cagtacaccc gaacgaacaa taaacgtgcc
    17401 taataggctt aaagcaaagg cgaaaatggc aagtagaatc gtccaataat agaaaatgcc
    17461 acgctgttgg ctcacaatta aactatgcac caatgcggtg ccaagtagcc aaggcattag
    17521 ggaggcattt tccaccggat cccaaaacca ccagccaccc cagccgagtt cgtaatatgc
    17581 ccaccacgcc cctaaaataa tgccggcggt gagaaatccc cacgaaatca tcgtccacgg
    17641 acgaagccaa cgcataatcg cagcgtccat tccgcctgaa acgagggcgg cgacaatcat
    17701 tgcaaagctc accgcaaagc cgacatagcc caagtagagc aaaggcgggt gaaagatgag
    17761 cccaatatct tgcaacattg ggttgagatc tcgcccttca ggtggtggtg gaaagccacg
    17821 ttcaaatggg ctggaaacca atagaatgaa aagtagaaaa cctagtgcaa ttagccctaa
    17881 catcgctaag gttcggttgg caaacaagcg gtcaattttt tgcgaaaatt tgcaaaatac
    17941 cgcagaccaa atcacaagag cggtgagcca aaacagcatt gagccttcgt gtccgcccca
    18001 agtggcggca aatttaaaaa aatcggggag ctggctgttg gaatgagcgg caacgtagat
    18061 aaccgaaaaa tcatcggtta gaaaagcata agctaaggta gaaaaggaaa atgtggtaaa
    18121 agcagcttgc aaataagtga aaagcggagc caatgccagc caatgatatt gcttgcgaac
    18181 ttcgcctaga agtgcaaaac caacttgaaa aatggcacta attgcagcca ataaaagtga
    18241 aaaatagccg agttccggta gcataaaaaa tccaaaaaat gaccgcttgt gaatgtaaaa
    18301 aaagccctct ccacttgtgg gagagggaca gatttttctt cgttcagaag aaaaatcagg
    18361 gagaggggaa ttaaataatt ttgctaaaaa taacaatact ccctctctct gaaaaatatt
    18421 tctgaacgaa atatttttcg gtctcaatac tcgctacgct cgctccttcg gagccgttgg
    18481 caaagccaac gttcaaatgt aaacatttgt cccacaaggg gagagagtat atcctaactt
    18541 gcaaccgtta gttgccctgc ataaaggata aagtaacgca agcagagtac accgattaag
    18601 tcgaaaacgg aaattaagat aataaatttc acgttatgtt tcaagcggtg gctaccaaaa
    18661 atattggcaa caagcggaat cgcaataccg attaacatta cgcctatcca gaacacattc
    18721 ccccagaagc cgttaagtgc atgggttaaa gcgagcgatt tatctgctcc accgaagtgt
    18781 aagcccacaa agaacgctac aattaagcct aattcggtta ccataatcgg cacttcaaat
    18841 ttgtgcataa agtggacttc gtggctttca ccggataact tgcctgcgag taaaatcact
    18901 aggaaagttg ctgcaatacc tgacgatgcc cctgatgcca agaacaatgc cggtaacaca
    18961 gggttattca gcattggata actcactaac gccgagagta agaaaccggt gtaagcacca
    19021 agtacagcag ccaatacgaa taagataaat tcaattggtt ttaacagctt cgcttcggca
    19081 aaatcgatca aaccagagac cacaggtaga taccgaacaa tccaatcttt aaacattatg
    19141 gcgatccaaa ccaccaatac tgccatataa agttggaata acattacccc catcgacatt
    19201 actgaggtaa agttatagtt gaacattaat ttccagaatg tccaaggttt ggttaagtgg
    19261 aaaatcagta acagtaagcc gataatggtt ggtaatgtgc caagaatagc cgcactacga
    19321 ataatccagt tttggcttgg tttttccatt ttgacaccgc tattacggta agcaatcgct
    19381 aactgaaccg cacctgatga aataccaagt aggaataagt aaatagcgat agtgccatcc
    19441 caaacaaggt ttggggtttg gaaaggtaca tattcattca ttatctatgt tctcctttac
    19501 caaatggaat gtgatataaa ttcggctccg tgcctaatgc cgttttggtg cgataaaccg
    19561 gattattttt cactttatgg aaaatatcgc tacttggatc attcatatca ccaaaggtta
    19621 aagctttagt cggacaagcc tctacacaag caggctgttt gccttctgca agattggtat
    19681 ctcggcaaaa gttacattta tccgccgatt ttttctcagg gtgaataaaa cgtacacgat
    19741 acgggcaaac cgcaatacag tattggcaac cgacacacaa atctgagtgt acatcaataa
    19801 tcccggtttc aggatcgata aatgaagcac cggtcgggca aacactcaca caaggtgcat
    19861 tggtacagtg ttggcaagat tggcggaaaa actcatattg tacgttcgga aactcaccga
    19921 aaggctcact acggataatt tctaaacgag aaaccccttc cggcactttg ttggtggtgc
    19981 gacaagcgtc catacaagcc gtacagccga tacaggcagt ttcatcatga accatcgcat
    20041 agcggatttt cttttgttct ttctcagtag caagagttat ggaggtggca agccctgtac
    20101 ttgccacaac tgcgatagca cctgcccctg agacgaaatc tctacgggtg caagtcatta
    20161 ttttgttcct tttgcttctg tggctttcat ttcaggattt ttaaattcgc ctgaatgaat
    20221 tttggtatga caatccgtac aaagtttaat gcgatctttt tcaggaatgt ctttcatcgg
    20281 atctttttcc gggtgaattt catgacagtt ggtacaagca attttagttg cgtgcgtgtc
    20341 gtgagcccag aatttttcac gcaatttctc tgccgtatgg caagcaaaac agacttgatt
    20401 ttgctcattt accgaacgtt caagtgatgg gttacttgct ttaccgtgtg ggttaaaacg
    20461 cattacgtct tttgcccctt tgcggtggct ttcagaaata ttaccgtggc aacttataca
    20521 agtgagtggc tctccattat tcgggctttt cacatctttt tggaaatgtt tgcctgcgtg
    20581 atggaaaggc ttacctgttg cgtttgccga ttcgtgacat tgagtacaat atttatttgg
    20641 atcacggcgt gcatcttgag ctacttttgc atcatcagcc gtttctgatt gccaaagagg
    20701 ttttggctgc tcaggcatta acgcattcgc actggaaatt gcaaaaaaac ctgcaaatac
    20761 gaccgcttgt atgagggcga ctgctcgtcc tgcttttgag attaattttt tcatagttca
    20821 cctcaagcta cattattttg cttctgcttt tggtgctgct accggtttgt ctgcttcttc
    20881 tgcgggtaat aaacctttag cacgagcttc tttttcccat tgaggaacca cagtttttaa
    20941 gaactcatct tttgctgctt gctctttagg aatatctaaa ccaatagcaa gttgtgcttt
    21001 ctctttagtt gagatgtccg gaatcgccac tggcgttgtt acaccgtgtt tcgctaacac
    21061 tacacctaat ttagcacggg catctgctgc acggtcgata cctgttgcga tggtacgtaa
    21121 cattacatca ggtgcgtgga agtggttacc gtgacctgcc gcagagtaat cccaacgcca
    21181 ttgagcgtga cgaattgcca ttaagatctc tttcatctct tcttgagttg ctccagcttc
    21241 ccaagcagct ttcgcttcaa agtgagcttt cactaattga tcttctaagc gaatcattgc
    21301 ctcttttact tcgtgtttat actcttttac acgtttttcc aatgtttctt tgctttgttc
    21361 atgacaagtt ttacaagtcg cttcaaattg gtcaaacgga ttaccgattt tgtggtcagt
    21421 ataaactttg ccgtctttgt ctttcacttt tggcatatgg caatcaatac aggttacacc
    21481 atttttccca tgtgtacctt gtgaccaaac ttcaaaatct gggtgctgcg tttttagcat
    21541 cggagctttc gataaagcat gagtccagtc tgtaaaattg atttcatcgt agtatttttc
    21601 aatgctatct acatctacac ctttatccca agggaaagtt acatctgttt tatttttgaa
    21661 atagtactcc acatggcagt tagcgcacac agcagggcgt ttaccatgtt tatctaaatc
    21721 ctcaaacttc caaccaaccg tattcaatgc acgtaatacg tgaggacgtg caacacgtag
    21781 tgcaggttta ccttgtttaa actcttctga tgtcgtgtca tggcagtctg cacaacctat
    21841 tgagtttaca atttcagcac catatttcgc ccatttagga tcaaaatatc ctcgctcacc
    21901 tttctcctca attaagcgtg gaacatcagg gcctttacat gtccaacaag ccataggttg
    21961 tggaccatca ttttcatctt taggcgcgcc ggtgcgcaaa atatcacgca catcggtaac
    22021 tgcataaaag tgacctcgtg gtttattata atctttcgca aaagcatagc ctgcccacaa
    22081 aataacataa cgagggtcag cctctaacgc acttcctcga tctgtggatt ttgcagttga
    22141 cttccaagaa tcgtattgta gcggatattt ttctgcaaat aggtggtttg cagactcaat
    22201 tttcacgcct aatttttcat gcttaactgc tttatctgtt aatgattcag ttttcgcctc
    22261 ttcagctaaa gcagtttgta aacaccccaa tattgaaatt gttgccacag ctaggctttt
    22321 tattaaaaat ttcattttgg ctctcccaat caggtaaaaa
//
LOCUS       DANLXQ010000021        21918 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.021, whole genome shotgun sequence.
ACCESSION   DANLXQ010000021 DANLXQ010000000
VERSION     DANLXQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21918
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.021"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(79..750)
                     /locus_tag="PXD94_000651"
     CDS             complement(79..750)
                     /locus_tag="PXD94_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL6285543.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
     gene            complement(871..2334)
                     /gene="guaB"
                     /locus_tag="PXD94_000652"
     CDS             complement(871..2334)
                     /gene="guaB"
                     /locus_tag="PXD94_000652"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL6285544.1"
                     /translation="MLRIKQEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTETKLAISLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISP
                     DMTLAELAELVKKNGFAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLV
                     TVKENASREEIFHLMHEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRL
                     RVGAAVGAGPGNEERIDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAG
                     NIATAEGAIALADAGASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKERGIP
                     VIADGGIRYSGDISKAIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATI
                     EELRTKAEFVRISGAGIKESHVHDVTITKEAPNYRMG"
     gene            complement(2562..5729)
                     /locus_tag="PXD94_000653"
     CDS             complement(2562..5729)
                     /locus_tag="PXD94_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL6285545.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVAATANSLTVAKDIYPMFEQVLKNLPSTIQGDIVYDKTIAIDSSIKEVI
                     KTIGEATVIVLVVIILFLGSLRAMIVPVITIPISLIGVLFFLQMFGFSINLLTLLALV
                     LAIGLVVDDAIVVLENVERHVKEGKTPFDAAIIGTREIALPVISMTITLGAVYSPMAL
                     MTGVTGTLFKEFALTLAGAVFISGIAALTLSPMMSSRILKEHKEGEESAFARVVHRSL
                     DKMTSCYTNMLSGIMAVRGFVLFFAVLIFASLPMLFGSLSSEVAPTEDRGFVVGISNG
                     PSNTNLDYTEAALAQFNQEVTKLPEVSSLMTVSGFNGANSALSIIALKDWNDRSRERA
                     AISNDVAAIGKKVVGMDVNAIAFPEISTGENDLPFSLVITTADSYEKLAIVASEFLKK
                     AQTSGQFFFANLDLKFDTVTMNMKFDREKMGAYGVTMQQVSGTLGAFLSGAIITRVDI
                     DSRAYPIVSQAVRNDRLNPEDLMNYYVTTATGQSIPLGSFVTMELKTSPSSLPRMSQL
                     NSATIGGVVSGSIGDAVNWAKAELDRTLPSGYQYDFRGESRQFVQEGNAMAMTFALAV
                     IIIYLVLAIQFESWRDPLVILVSVPLAVSGALLVMNIPTAMKGLFMGIAAGTGSKDMM
                     AFANGIWTAGYTLNIYSQVGLITLVGLITKHGILMCEVAKEEQLLHGKSRYEAIFHAA
                     TIRLRPIMMTTAAMIAGLIPLLFAVGAGAIARFSMGMVIVAGLAIGTLFTLFILPVIY
                     TFLGQEHKPLREFDEEKYFPTTKEA"
     gene            complement(5743..6948)
                     /locus_tag="PXD94_000654"
     CDS             complement(5743..6948)
                     /locus_tag="PXD94_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL6285546.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGVVSRVLVTSGQRV
                     KKGDLLVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEFDNAQSTYNQ
                     LLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQSSMKVRFTLP
                     QTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVIEEGQEKLLS
                     GMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNKMAVQNPSLD
                     VNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVKVSDTEAVGI
                     TTPAKNSKL"
     gene            complement(6979..7551)
                     /locus_tag="PXD94_000655"
     CDS             complement(6979..7551)
                     /locus_tag="PXD94_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL6285547.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQFLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLSNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            7790..8011
                     /locus_tag="PXD94_000656"
     CDS             7790..8011
                     /locus_tag="PXD94_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL6285548.1"
                     /translation="MATKSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNI
                     LIENIQSPAQREALADAFADALKQSLKTK"
     gene            8016..9749
                     /locus_tag="PXD94_000657"
     CDS             8016..9749
                     /locus_tag="PXD94_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL6285549.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(9816..10517)
                     /locus_tag="PXD94_000658"
     CDS             complement(9816..10517)
                     /locus_tag="PXD94_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6285550.1"
                     /translation="MIGQALGWRATFGIIGIIALIVLMMIWRLLPYLPSKNAGSIKSL
                     PLLFKRPMLIGIYLLVALIVSGHFTAYSYIEPFMIKISSVSVENTTFILLVFGLAGVV
                     ASFLFGRLYGKAPNKFILSGIAIIMCSQLCLLFLSDSTVAMFMVAFVWGIGITGMSIA
                     LQMKVLELAPDATDVATAIFSGTYNLGIGGGALLGSIAIQQLGLANIGFAGGSIAFVA
                     MVWFVFVSLKYRQLR"
     gene            complement(10514..10894)
                     /locus_tag="PXD94_000659"
     CDS             complement(10514..10894)
                     /locus_tag="PXD94_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6285551.1"
                     /translation="METLAIFLFSEIWKFDFVFTFIFKILKINVFFSDLINMKLARKA
                     QRRLSFLRVFSLAFAAFIVNTTEFIPIALLSDIASSFQMEPSSAGIMMTVYAWVVFLL
                     SLPLMLLTANMERKRLLLMLFMGE"
     gene            complement(10936..15396)
                     /gene="mukB"
                     /locus_tag="PXD94_000660"
     CDS             complement(10936..15396)
                     /gene="mukB"
                     /locus_tag="PXD94_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL6285552.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTALIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAELALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDHVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFELVCKISGEID
                     RLQAWEEARALLSAFPEQKMQAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQ
                     QLNSAEELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWH
                     TAQSALTRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQMLDEQINRL
                     SQPDGSEDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVK
                     TQLEKLDDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFG
                     RAAREKHLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEML
                     MQEIAAERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARA
                     EECREQLELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVL
                     QQKVFSLADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQ
                     LSQYNQVLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQ
                     RSRKGYLDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDV
                     EKRLNRRELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFF
                     IAVYQHLRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILR
                     KTIQREQQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDS
                     QKLSFSEALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAE
                     SSALSTGEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTL
                     FELCERLDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGFAK"
     gene            complement(15572..16288)
                     /gene="mukE"
                     /locus_tag="PXD94_000661"
     CDS             complement(15572..16288)
                     /gene="mukE"
                     /locus_tag="PXD94_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL6285553.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKAGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(16337..17680)
                     /gene="mukF"
                     /locus_tag="PXD94_000662"
     CDS             complement(16337..17680)
                     /gene="mukF"
                     /locus_tag="PXD94_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL6285554.1"
                     /translation="MQNELAQTIPELISWTKDREFSLSLSSDRLAFLLAISIYNQEQT
                     DGELLESDLIDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIY
                     RVTPLGVGISNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNN
                     VFAPLKYSVAEIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSG
                     NLRELQDTLNAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAI
                     DLWIGYDRHVHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDD
                     EAMLNEAESVGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLA
                     RFPESRHFDVARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
     gene            complement(17945..18832)
                     /gene="galU"
                     /locus_tag="PXD94_000663"
     CDS             complement(17945..18832)
                     /gene="galU"
                     /locus_tag="PXD94_000663"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL6285555.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLILADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGYKVGYMKAFVEYSLN
                     HDKCGKEFKAFIKELAKSL"
     gene            complement(18968..19153)
                     /gene="csrA"
                     /locus_tag="PXD94_000664"
     CDS             complement(18968..19153)
                     /gene="csrA"
                     /locus_tag="PXD94_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL6285556.1"
                     /translation="MLILTRKIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHR
                     REIYERIKASVEEENAE"
     gene            complement(19243..21870)
                     /gene="alaS"
                     /locus_tag="PXD94_000665"
     CDS             complement(19243..21870)
                     /gene="alaS"
                     /locus_tag="PXD94_000665"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL6285557.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGAKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKASSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
BASE COUNT         6210 a         5038 c         4125 g         6545 t
ORIGIN      
        1 ggttactcaa gccgatagac tgttccaagt acttgaagta gaaacattga caaaatttct
       61 ataaaaatcg accgtttatt aacttggagt attacatttt tgaagaatct tcaaaatctc
      121 tttcatatct ttgcccatat aatccgctaa actttggtca atcagtattc gtttttcttc
      181 gtaaccaatc accgttaagc ctgcggtttt ggcggcgagc attccgtagt aggagtcttc
      241 aatcgccact gcattttgtg gctcaacgcc taatttttcg caggtgtagc ggtaaatggt
      301 tgggtctggc ttgctgtgtt caaactgttc accgctgacg attaaatcaa attcgtcttt
      361 aatgccgcaa accgtgagaa tattttcaat atgcgaaagg gcggaagaag aggcaaccgc
      421 aagcttaatt tgattctgct tggcaaattg aataatttgc tgaatatcgg ctctgaaaat
      481 ggttttaaaa tccactttgc taaacagatc ttggaaaaag gcgtagtaac gtgcacgaat
      541 ttcctcaatc ggcaaactgg attcgcttag ttttttgata atcaccggaa tttcttttaa
      601 acatttgcct accacttccg attgttgttc taaagtaatc gggcggctgt gttctttgat
      661 gtcttcaata aactgttttt gtaaggaaaa ttccagatat tcggtatcca caatcacgcc
      721 gtccatatca aaaatgatag cttggagcat atagcctctt ttgtaaaatt ttaagaaaat
      781 ctaaccactt attccctctc tctgttttaa attactgaac gtaatttaaa acggtctctc
      841 tcccacaagg ggagagagtg agaaaggcta ttaacccatt cggtagttcg gtgcttcttt
      901 ggtaattgtc acatcgtgga cgtggctttc tttaatacca gcaccactaa tgcggacaaa
      961 ttctgctttg gtgcggagtt cttcaatagt ggcacagccg gttaatccca tacaggagcg
     1021 taagccgccc atttgttggt ggataatttc tttcaagtag cctttgtaag cgatacgccc
     1081 ttcaatccct tccggtacga gcttgtcggc ggcattatca gattggaaat agcgatctga
     1141 cgagccttta ctcattgcac ctaatgatcc cattccacgg taggatttga atgctctgcc
     1201 ttgataaagc tcaatttcac ccggggcttc ttctgtgccg gcaaacatcg aaccgaccat
     1261 tacgcaactt gcaccggcgg caatagcttt tgaaatatcg cctgaataac gaattccacc
     1321 atcagcaatc acaggaatgc ctcgttcttt aagcgcagct gccgcttctg cgattgccgt
     1381 gatttgtggc acgccaacgc ctgttacaat tctggtggta caaattgaac ccgggccgat
     1441 tcctactttc acagcactgg ctcctgcatc agctaacgca atcgctcctt ctgcagtggc
     1501 aatattaccg gcaacaatcg gtaaatcagg gtattttgca cgggtttcac gcacacgttg
     1561 taatacacct tcagaatgcc cgtgcgaaga gtcgattaat agcacatcga cacccgcttt
     1621 tactaaagca tcaattcgtt cttcattgcc cggaccggct cccactgccg cccccacacg
     1681 caaacgccca aactcatctt tacaggcatt cggtttgctt tccgcttttt ggtagtcttt
     1741 tagggtaatc attcctttta actgaaattc attattcact accagcactt tctccactcg
     1801 gtgttcgtgc attaagtgga aaatttcttc acggcttgcg ttttctttta ccgtcactaa
     1861 acggtctttt ggtgtcataa attcacgcac tgttttgctt aaatccgtga caaatcgggt
     1921 atcacgtccg gtaataattc ccactaaatt ttggttttca tcaatcaccg gatagcctgc
     1981 aaaaccgttc tttttcacca attcagccaa ttccgctaat gtcatatcag gagaaatcgt
     2041 cactggctca gaaacaatac cactttcaaa ttttttcact ttacgcacac ggtctgcctg
     2101 gcgttcaatc gacatatttt tatggataaa accaatgccg ccttcttgag caagggagat
     2161 cgcaagctta gtttctgtaa cggtatccat tgcagcagaa agcatcggaa tgtttaaacg
     2221 aatggtttta gttaattgag tagaaagatc agcagtatta ggaagcacag tagaatgtgc
     2281 cgggacgaga agaacatcat caaaagtgag ggcttcttgt ttaattcgta gcattgcaat
     2341 tattccttaa aaaatgatta aattgaaaat ctgtactgga aaattgcgag cggattatac
     2401 agaggaacta ggtttctgta aatagggaaa cgtttacgct atctgaaaat atactttcaa
     2461 gcggttaaat tttgtgattt tttagcaaac aataaataaa aaacgaccgc ttgtaagcca
     2521 acaaaaaaga gcgtatcgct acgctctttt gctatttaaa actatgcttc ttttgtcgtt
     2581 gggaaatatt tttcctcgtc aaattcacgt aatggcttgt gttcttgccc taagaaagtg
     2641 tagataaccg gcaagataaa cagagtaaac aatgtcccga ttgccaagcc tgctacgatt
     2701 accataccca tactgaagcg ggcgattgca ccggcaccca ccgcaaacag aagcggaatt
     2761 aagcctgcaa tcattgctgc cgttgtcatc ataattgggc gtaaacgaat cgtggctgcg
     2821 tggaaaatag cttcatagcg tgatttgccg tgcagtaact gctcttcttt cgccacttca
     2881 cacatcaaga taccgtgttt agtaattaag ccgacaaggg taattagacc tacttgtgaa
     2941 tagatattta aggtataacc tgccgtccaa ataccgttgg cgaatgccat catatctttc
     3001 gagccagtgc ctgctgcaat ccccataaac aagcctttca ttgcggttgg aatgttcatt
     3061 accaacaatg caccactcac agctaacggc acagaaacca aaattactaa tgggtcacgc
     3121 caagattcaa attgaattgc aagtacaaga tagataatga taactgctag agcgaaagtc
     3181 attgccatcg cattaccttc ttgaacgaac tggcgagatt caccacggaa gtcatattga
     3241 taacctgatg gtaaagtgcg atctaattct gctttcgccc aatttaccgc atcgccgatt
     3301 gagccagata ctaccccacc aatggtggcc gagtttaatt ggctcatacg aggtaaagaa
     3361 gatggtgatg tttttaattc catcgtcaca aatgaaccta acgggataga ttgccctgtt
     3421 gcagtcgtga catagtaatt cattaaatct tcaggattta aacgatcatt acgcaccgct
     3481 tgcgacacaa tcgggtaagc acgagagtca atatcgacac gagtaataat cgcaccggat
     3541 aagaatgccc ctaatgtgcc actcacctgc tgcattgtca cgccataagc acccattttt
     3601 tcacggtcga atttcatatt catcgtgacc gtatcaaatt tcaaatctaa attcgcaaag
     3661 aagaactgac cggaagtttg ggcttttttc aagaattcag aggcaacgat tgccaatttt
     3721 tcatagctat cagcggtagt aatcactaat gagaatggta gatcattttc accggttgaa
     3781 atttcaggga atgcaattgc attcacgtcc atacccacca cttttttacc gattgcagcc
     3841 acatcattag aaatcgcagc acgttcacgg ctacgatcat tccaatcttt taatgcaata
     3901 atcgataatg cactatttgc accattaaag cctgaaactg tcattaagga agatacttca
     3961 ggtaatttag ttacttcctg attaaattgg gctaacgctg cttcggtata atctaaattg
     4021 gtgtttgaag gtccattaga aataccaacc acaaagccac ggtcttctgt cggtgcaact
     4081 tcactggaaa gtgagccaaa taacatcggt aaactggcga agatcagcac cgcaaagaat
     4141 aagacgaaac cacgaacagc cataataccg cttagcatat tggtatagca gctggtcatt
     4201 ttatctagtg atctatgcac cacgcgggca aatgctgact cttccccttc tttatgttct
     4261 tttaatattc ggctagacat catcggcgaa agggttaatg ccgcaatccc cgaaataaat
     4321 accgcaccgg caagcgttaa agcgaactct ttaaatagtg tccctgttac cccggtcatt
     4381 aatgccattg gtgaatatac tgcacccaga gtaatagtca ttgaaataac cggtaaagca
     4441 atttcacgtg taccgataat cgctgcatca aatggcgttt taccttcttt aacgtgacgt
     4501 tctacgtttt caagtacgac aatcgcatcg tccactacca agccgatcgc taataccaac
     4561 gccagcaaag taagcaagtt gatcgagaaa ccaaacattt gcaggaagaa taataccccg
     4621 attaaggaaa tcggaatggt aatcaccggt acaatcattg ctcgtaatga acctaagaac
     4681 aggataatta ctaccaatac aatgacggtt gcctcaccaa tagttttaat tacttctttg
     4741 atagaactat caatcgcaat ggttttatcg taaacaatat ctccttgaat ggtgcttggt
     4801 agatttttta atacctgctc aaacatcgga taaatatctt tcgctacggt taaagagttt
     4861 gccgttgcgg ctaccgaaac agcaagcaca actgcatctt taccgccaac cgttgctctt
     4921 gctgcatctt ggtatttatc cagctcaaca gtcgcaatat cttttaattt gatggttttt
     4981 ccatctttgg tggttgaaac cgtaacctct tctaactctg tcacagaagg cgtggtagaa
     5041 agtactttgt ttctataaac cgtatagtaa ccgttagaat tacccgcagc ggtttgcaag
     5101 ttattggcac ttaatgccga taatacagcc gcccctgata ggttattgct cgccatttta
     5161 tccggatcaa gccagatgcg taagccaaaa acagagccat acacatcaac gctagaaaca
     5221 ccatttaccg taaagaactg tggttttact acacggttaa tataatcggt aacttgtgat
     5281 gtattaagtt catcagagac aaatcggata tacattaacg catcatttga accggttgag
     5341 actctaattg acggatcatc aataccgctc ggtaaatttg agcgtaccgc attcactttt
     5401 gccgaaatat cggaaagtgc ggcattcgga tcggtattta acttcatttt tgccgttacc
     5461 gtagtggtac ttggagagct tgatgaagtc atatagtcaa tattatccgc ttgagcaaca
     5521 gcctcttcta actgtgaggt aaccagtgtc tgcattaaac gtgcatctgc acctgaatag
     5581 ttaacactca cggttacaat cgagaccgtc atctccggat attcacgcac ctgcaatttt
     5641 tcgattgctt gcaagcctag gatcgtaatc aataggctga tacaaactgc caaaacagga
     5701 cgtttaataa agatgtcagt aaatttcact taactcactc cactacagtt tactgttttt
     5761 cgccggtgtg gtaatgccta cggcttctgt atcagaaact ttaaccaacg cattattact
     5821 taaacgttgt aaaccgcctg ttacaatcag atcacctgct ttcacgcctt tggataattg
     5881 tgaatagttt cctttgcggt cagccgtttt cacttccatc tgttttgcac ggtacatttt
     5941 gtttacatct aaacttgggt tttgtaccgc cattttgttc accatttctt tatcttcatc
     6001 agataatggt tgcagcacat ataccgtttc accatacata gtgtaagcga tagaaatttg
     6061 tggtacgacg atttgtccgg tttctgttgg taaagcaaca tttaagcggg caaacatacc
     6121 tgaaagcaat ttttcctgcc cttcttcaat caccgcttcc acattaatta agccggttaa
     6181 acgatccaca gccggatcaa tagccacaat tttagctggg aaggtttgtg ccggtaacgc
     6241 atcaattacg gctgtcactt tttgcccaat cgagatttgc tctaaattag tttgcggcaa
     6301 ggtaaaacgt actttcattg aagattgatc ttccacacgc acaatttccg tacccattgt
     6361 aatgtattgc ccaacattca cattcacgat acccgctaaa ccactaaacg gagcataaat
     6421 ttgacggcgt ttaatcgtag cacgcaatgc ctcgatattg gctaataatt ggttgtaggt
     6481 agattgagca ttatcaaact cggctttaga agcactgttt gaggcaatca gattacggta
     6541 acgctctaaa tttgctttgg cattcggtaa ttgcgcttca gaggctctca agttagcaat
     6601 ttcaacactg ctgtcaaatt ctactaacag atcacctttt ttcacacgct gtcctgaagt
     6661 gaccaaaaca cggctaacca cgcctgaagt ttcagcactt aacattgccc cttgatttgg
     6721 gcgaatataa ccgaccgcag aaatggttgg tgtccattct tgggtcgtca cttgcatagc
     6781 agtaacttcg ctcaccgcct ccggcatatt tgccgctgct tcggcttttt tcattgcccc
     6841 gattttttgt aaccctacga ttgcagcaaa cacaatcaat acgattaaca atgtgagtac
     6901 aattaaaaac tttctgcctt tacccagctt ctgtgtttcc gttgtcatac attctccata
     6961 aaactaataa aaagaaactt aaaaagtgat tgctttccaa gtgcgtaaaa taatctcgtc
     7021 taaatatcca tcaaactgat tttcttgatt cagattttgc agatattgga tatccaccaa
     7081 tgtgccgatg cttaatccat aaagcaactc attagataga tcagcaataa tcctttgctt
     7141 tttcccttct aaaattaatt tattccaaaa ggaagtttca tcagctatgg tttcccgaat
     7201 caattcatca atccctatca ccgcttgaaa ctgttttacc aaatctaaac gctcgattaa
     7261 aaactgccat tttttattga ccaacgcttt atattgctca aataacgcca tttcaggctt
     7321 aaactcaaaa tgcatataac cgttataaca ttcgagtaat tgggcagcca atgagtgcaa
     7381 taaatcatct ttggttttaa agtaaagata taatgtaccg gaagcgatcc ctgcttcttt
     7441 tgcaatacta cgcattgaaa ggttttgcaa accttcttgc acaatcagcg actcggttgc
     7501 cgataaaata cggtttacca tctcttcttt tggctcgctt cgcctagaca ttttatctcc
     7561 ttacctataa tgaacagccg ttcattatag ctttttttat gaaaaaagaa agcagaaaac
     7621 acctatcaaa ttaaaataaa atagttgcaa aattcctcat ttattgaaca agcggtcatt
     7681 tttggctaaa actttgcaaa aatcactcaa acaataacgc ttaacgctac tttattatta
     7741 tttttgtgct actatttgta cctaattata ggatcatctt tatttcaata tggcaacgaa
     7801 atctaaatac cacaccaaac aatttgagca gctattggct gacctcatcg ccaccataga
     7861 aaagcataaa gcaccggtgg atttatcgtt aatggcatta ggcaatatga taactaacat
     7921 tttaattgaa aatattcaaa gcccggcaca aagagaagct ctcgctgatg cctttgccga
     7981 tgcattaaaa caatcactaa aaaccaaata agatgatgtt tgcacgccta cgcagtttat
     8041 tgccgaccaa ttctcgccaa tatcgagaag aaacctcaca gaaaatcacg tgggggcatt
     8101 ggtttgcact atttaatatt gtgttggcac tactcatttc ctctcgttac gcatttaatg
     8161 cagactggcc gaacacgctt gtaggcaaac tttatttctt tgtcagcctg ttcgggcatt
     8221 tcagctttgt ggtctttgca ggatttctat taattctgtt tccgctcagt tttattgtta
     8281 agaatcatcg cacctatcgt gggctttccg tgattttagc caccatcggg caaaccgtgt
     8341 tattggtgga tactgaagtc ttcaagcaat tttatctgca tttatcaccg cttgtatggg
     8401 atctgttagt taatccggaa gaaggggaac tgacccgtaa atggcaactg ctgtttgtac
     8461 caatgccact ccttttgctg gcagaaatgc tctactctcg ctggtgctgg cagaaactgc
     8521 gtagttttag ccgccaaaaa tggggcaaat atgtcgctct ctttttcgtg atcagcttta
     8581 ccgctactca tttaatctat gcgtgggcag atatgaatgc ctaccgcccg attaccgctc
     8641 aacgagcaaa ctacccactt tcttacccga tgacggctcg taatttctta gaaaaacacg
     8701 gttttattga ccgagtagaa tttaatcaag aaattgaaga aagtggcaga ctagatactt
     8761 tctatctgaa ttatccaaaa tctcccttga ttgcgaccaa aacgccctca actaatattt
     8821 tactgattaa tttatcaggt ttatctaaac agataataaa tgccgaaaca atgcctaagc
     8881 tgacagaaat cagccaacaa agccaccgct ttatgcagca ttacagtagt ggtgattcag
     8941 cctctgccgg tgcattaggt ctattttaca gtttaagcgg taaatatttg gattccgttt
     9001 tagccgataa aaccgtgtca ccgcttatcg aaatcgctag aaaatcggac tatcaattag
     9061 ggctgttttc acataatggt tttgccgacc cgatttatca tcaggcgatc ttctctggta
     9121 ttttattgcc tacggcgcac agtaatgtag gggcgattgg gcaatggaaa aactggatca
     9181 gctctcgctc attggaaaat ccgttcttta gctatttaga cttaagaatg cccttcgccc
     9241 acaccgctca agagcaggca aaattattag attggcaatt cgctgaaatc tggcaacatt
     9301 tgacccaaaa tgcactattg gcgaatacgc tggtaatcat taccgccgat attagtaatg
     9361 aagttggagc agaaaatagc ttcgataaac aacacattca agtgccgatg atgttctact
     9421 ggcaaggcga aagcaaagag taccatttcc tttcaagcca tttagatatt atgccaacct
     9481 tactgagcca attcttcggc gtgcaaaatc cgctgaatga ttatgcacaa ggtatggatt
     9541 taagcaccca gaaccaacgt ttatgggtat ccagttctaa tcacaaatgg aatgtggcga
     9601 ttatgccgaa tggcgatcaa tatcaaattg atcgtaaagg caactttaag cactttaaca
     9661 gcaatggcga acacgagaaa gatgctcgac caccattagc gttgttctta caaatgattc
     9721 aacaaagcaa tcaatttatt gaaaagtaat gttcaacaag cggtcatttt tgcaggaaaa
     9781 tttgcaaaat ttcagcaaaa tatgaccgct tgtcactatc tgagctgtct atatttcaag
     9841 ctaacaaata caaaccaaac cattgctaca aaagcaatac tcccaccggc aaatccgata
     9901 ttggctaatc ccagttgctg aatagcgatg ctccccagta atgctccacc gccaatgcct
     9961 aaattataag tgccggaaaa aattgcagtt gcgacatcag tcgcatcagg ggcaagttca
    10021 agcaccttca tttgcaacgc aatgctcatt ccggtaatgc caattcccca gacaaaagcg
    10081 accataaaca ttgccactgt tgagtcactc aaaaacagta aacaaagctg gctgcacata
    10141 ataatggcga tcccgcttaa gataaattta ttcggggctt tgccatataa ccgaccaaat
    10201 aaaaaacttg ccaccacacc tgccaagcca aaaaccagca aaataaaggt ggtgttttcc
    10261 acactcaccg aactgatttt aatcataaac ggctcaatat agctgtaagc ggtaaaatgt
    10321 cctgaaacga tcaatgccac cagcaaataa atgccgatta acatcgggcg tttaaataac
    10381 aaaggcaaac tttttatcga gcctgcattt ttgcttggta aataaggcag caaccgccaa
    10441 atcatcatta gcacaatcaa agcgataatc ccaataatgc caaaggttgc ccgccagcct
    10501 aaggcttgcc cgatcattcg cccataaata acatcagcaa cagccgtttt ctttccatat
    10561 tcgccgtgag taacatcaac ggtaacgata ataaaaatac cacccacgca taaaccgtca
    10621 tcataatgcc tgccgatgaa ggctccattt ggaaactgct tgcaatgtct gaaagcaatg
    10681 caataggaat aaattcggtg gtgtttacta taaaagccgc aaaggcaaga ctaaatactc
    10741 taaggaacga taatctccgt tgagcttttc gagcaagttt catattaatt aaatccgaga
    10801 aaaacacatt tattttcaaa attttaaaaa taaatgtaaa aacaaaatca aatttccaaa
    10861 tttctgaaaa taaaaagatt gccaatgttt ccatcaacaa tctttttata catatttaga
    10921 ttaaagtgct aagttttatt tagcaaagcc ttttaaacca acaacgtgaa catactcttg
    10981 gttattggtg attttacgca ccaatttata agttgtgcct cgttcagggc taatattttc
    11041 aggggctgca attaataact gcatatccaa acgttcgcag agttcaaata gcgtattaat
    11101 tgaggtagca tctaatcgag ccgcttcatc taagaacaat aagcgagccg gtagaatatc
    11161 tttggcacgc atacggcgag cttcttcttc ccagctttga actaccatta agagaatcga
    11221 catacctgta ccgatcgcct caccggtcga taatgcacta ctttctgcac gcatccagcc
    11281 gtctgcacca cggaaggttt ccacttctaa atctaagtag ttacggtaat ccaataactc
    11341 ttcaccaatg gtctgtggtg tacgttgccc catctcaata tgagggttca cccgtttata
    11401 caacatcgct aaggcttcgg agaagctcag tttttggctg tcgaataaat ctttgtgttc
    11461 ctcacgatta ttggataacg cattgagtaa aatagcgtgc gtatctcgga tattcaccac
    11521 caaacgcacc ccttttacct gaccgaatgc aatattttgt aaaccttggt tcagttgcaa
    11581 aatgcgttgt tgttcacgct gaatggtttt acgtaaaata tttgccacac tttccgcact
    11641 gatcgccagt tttttctcac gaccggttaa ttcattggtt aagcgtgaaa gctcgatttc
    11701 catctgctca atcgcatcaa tcggatcatc ggttttgata atgtcttggc gaatccgctc
    11761 acgcaaatgc tgataaaccg caataaagaa cgccactttg ttttccggct tgcgactatc
    11821 ttcagaagcc cgcaagctat cacgcaggta ttcattatcc gccacggcgg tgcgtaatgc
    11881 cccgagagct ttatccgaaa tcgaacgtaa ttcttcagcc gattgataag ccaattcacg
    11941 gcgatttaaa cgtttttcca catcgctgtt acgagataaa cgttgcacaa tacaccagct
    12001 ggcttttgcc tgcaccacca attcacgttg tgtatgataa tcacgttcgg ctttacgtaa
    12061 ggtgcggtta aggttatcca tttccgcctc gatcaccgca atttgtttat ctaaataacc
    12121 tttgcgggaa cgttgctggc ttaaacgttg ttgcaattca tcacggcgga atctggctcg
    12181 ctcttccgct ccgctatcac cacgcacgcc caaatcgtca atttcacgaa ttaactcttg
    12241 tagcatttgg tttttcgcct cataagaact gcgtaagccg gtcaatactt ggttatattg
    12301 tgaaagttga gcttgagctt gacgcagctg atcacgagcc tgttcacgct cacgttgtgc
    12361 attttccaaa cgttggcgta gctgttcggt taatgcagag ccttctgtac ccaccgtttc
    12421 ttgatagcta aaatggaggc ggcgattcat tacatcagcc aagctgaaca ctttttgctg
    12481 taacactttt tgctcggcaa ctgaacggcg gtaatcggct tccaactgtt caaataaagc
    12541 cggatcactt tttaatgcgg ctgcaatcgg ctcaagttgg cttaatgttg ctccaaattg
    12601 gcggataaac atttcatctt cttctgccaa ttctaattgc tcacgacatt cctccgctcg
    12661 tgcttccagc gtttcatcgg ctaataaatt cgcaagcggt agaattttat tcaacatttg
    12721 caactgggct tttacgcttt ctaattggtg acgtaattgc tgttcgttac cggctgattg
    12781 tgccaattca cgttcaattt ctgcccgttc tgccgcaatc tcttgcatta acatttcagg
    12841 gttttcttgg aacgctaaat ttaagtgcgt gccgataaat tggcttaagt gttgatgtaa
    12901 acgctggcat ttctgcacat caaaggcacg ttcagcgtgt ttttctgcgg tttcatcacg
    12961 ctctgctttt aaagtcgcta aatgtttttc acgagcagca cgtccgaata acggcacttc
    13021 agggaattta gaataacgcc attggcgatc ggagactttc accaccacgc cttcgcctaa
    13081 ttcctcggca ctaaataccg catcgtcaaa ggcggacgga tcaccttcaa ttaaataaag
    13141 atcacttggg caatcgtcta atttctctag ctgtgtttta accgcctcta aatcacgcac
    13201 tacaatagcg tggcgagcct cgccgtataa ggcggagaaa taaggcgcat cttcaattga
    13261 tacatcatca tataactcag agagtaacac tccaccaaaa cgttctgcca actggtttaa
    13321 gcgggcgtct tccgagccgt ccggctggct taagcgatta atttgctcat caagcatttg
    13381 ttctttacga gccaattcat cacgagcaag ggtggcttca cgctctttgc tcagcatatt
    13441 ttgcataaac tgcatcactg attggctggc ttcaaacttc tcgccacatt gctcttctaa
    13501 acgagtcaga gccgattgtg ccgtatgcca agccggtgca ttcttcgcta attgctgata
    13561 ttgttgatta agctgttcac gttgttggcg ttgggttgaa cgttgttcta caaagtccga
    13621 aagctctgcc tctaaatctt ctaggcgagc ttgctgctct tcaaaatagg cgtctaattc
    13681 ttccgctgaa ttgagttgtt gttgcgattt ttggttaaat tccgccacta aacgctgggc
    13741 attttgctgt tgttgcaaac gctgttctaa atcgttgagt ttttgacgta aactcaccgc
    13801 ttgctgagct tgcatttttt gctcagggaa ggcagaaagt aacgcacgag cctcttccca
    13861 cgcttgcaag cggtcaattt caccggaaat tttgcaaacc aattcaaacg ctttttcaaa
    13921 ttgggttttc gccatatccg acacgcttaa acgttgttcc agctcaaata cttgatcggt
    13981 aagatcttcg gcttgagcca caaattctgc gtggtaatct tcaatattat ttaaatctaa
    14041 attcactaag ccggttagct gtttggcttt ttctaacgca ttcaccgctt gttggtattg
    14101 caatgcacga gtttgctgtg catctaaggc ttgttggtaa tccgccattt gagaacgcaa
    14161 ttcttcaacg tgatcatctg cctcatccgc acgagcctga gcattttcgg caatttccgc
    14221 aatttcttcc aatgcctctt gctgttcttc cagcttaata ttcagctctt caacttcgtc
    14281 ttggtagcgt tcgattttct cttggtggcg taacgcattc atcactaaat ttagatgatc
    14341 gttcgcacta ttgtaatccg cctctaacgc cagctctgct tcggcaatat ccgcactttc
    14401 acggctaaat tccacaaagc gttgctgttc gagcaaaatt ttggattttg ccgcatacca
    14461 ctctttgcgt tgctctaagg cttgttgtac attgccacgg cgttcattcg cattacgcat
    14521 ataatccgcc gacacataat tggtcgattc ggtaattaag cgtttgaaca tatcccgatc
    14581 cgattgcgtg accttaatcg cctctaaggt catacggttt tcacgcagag cggattccat
    14641 atcttggaac gcttggcgaa cgcccgtatt ttccggcaac aagtaatcac gcaaggattt
    14701 ggtaatcaca cttgaaatac caccataaag cgaggcttcg attaatttgt agaatttgct
    14761 acggtcggct gccgaacgaa ggcgttttgg gattacgccc aaatcgaaca taaaactgtg
    14821 gtaatcagta atcgaatggt actgtttgaa ttggacttcg ctgttctcaa attgatcttt
    14881 gagttcattt aacgccagca ctcgagcctt attccccact tgttcggtca gtaggctgat
    14941 aatgctttgc tcttgaggaa tattctggag cgagaacgaa cgaatatcga cttttttatc
    15001 ccgtccgaca atttgttgca aacgcacgcc ggtaataatc cgttgcccac gggaattgaa
    15061 tgactctaac accgcatagc aaacacccgc ttttaactta ccgtataagc ctttatcacg
    15121 agaaccggcg gttgagcctg cttcagtcgt attacggaaa ttaagcaagg ttaaatctgg
    15181 gatcaaggcg gtcacaaacc ctgccattgt ggtggactta cctgccccgt taccgcctga
    15241 aagcgtggtc actaagtcat ctaaatcaaa ggttcgagca aaaaaaccgt tccagttaat
    15301 cagcgttaaa gagagaaatt taccgcgttg tacatcattc gggcgtgcaa gcggtgcaat
    15361 ttgaggatta ttttgcaatt ctgtggtatc aatcattatg ttttctcact aattgtccta
    15421 gcacgctaaa gcgtactagg ttaggtatag gtgagtagct ccgctactct aatcgcaacc
    15481 tcagccttgg agggctgctt tatgcttaac ctagttcggc tttaccgtgt ctagggcaga
    15541 ctcattaaat gctgaatcta ttcgaatgaa atcactcatt ttctccgtca aagggggctt
    15601 catctgcaat attttcatct atttcaaccg cttgttcgtt taacaacgca ggggttgtcg
    15661 cttcaccatc acgaattaag cgaagctgtg cctctcttgg gtcatcaccg gtacgcacat
    15721 cagccccaaa acggaagacc gattctgaaa tcacaaattt tttgctgttt tgctcgccta
    15781 ctcgggtaat aatgccgata cgggctaatc tgcgtaacgc tccgccggct ttttctgcca
    15841 gcttggcacg gtctaaatcg gaaccggtcg aacgtgggtt taccgctttt aataatttgc
    15901 tttcatcggc aagactgagt aattcttcat acacatcatc ttggctgaaa atcccctgct
    15961 gagccaaacg ttccggactt aaatagaggt agcacaacac cttacccact aacatttcca
    16021 tttctgacat tgccgaacgg gcaattagtg tcgaggcttt cgggcgtagg tagaaaaagc
    16081 cttccggtgc tcgaatcaac tcaacgtgat aacggcggta aaaactctct agttccgctt
    16141 gaaaatccat caaaaagacg tgttggtcaa gctgctcggt attgatatgg cgaccggctc
    16201 gtaattggct gtctagctcc ggaaaaatcg ggttggcaat cgcaagtgcc agtttcgggg
    16261 aaatgaaatc ttggatattc tctgtcattg tcattctctt caattttgca aaattctggg
    16321 taaaaacgac cgcttgttat ttattgtatt ggtcgataac attcgcctgc acttccgccc
    16381 cttgctcgtt aatcggctgc cattgtgggt aaacggcttg gctatcttgg cttgccatac
    16441 ctaacttaac agcttgatcg acaataatac gagccacgtc aaaatggcgg gattccggga
    16501 atcgggcaag ctgttcacgc aagataacgc ctaaatctat cggtttacct tcagcacgga
    16561 acggcgctaa atgagcttgc attgccgaaa caatttgctc ttgcacatct gaaagcgact
    16621 catactctaa ctcacttggc aactcgccca ccgattctgc ctcatttaac attgcttcat
    16681 catcacgcaa gtccagtaac gaatcggctt ttgcagtata aagcaaccaa gggctgttgt
    16741 aataatcttg aatcgattgg cgtaaccgtt gcccgaatac acggtttttg tccatatcaa
    16801 tcgctgtacg aataaattta tgcacatggc ggtcatagcc gatccaaaga tcgatagctt
    16861 gttgtcccca gctgataata cggtctaact tattttgtag attcacaatc agctgatcga
    16921 taaaatccaa atcattacga gccatcaggc aactttgaat ccgcaataat tgagcttgca
    16981 gtttatcacc agcggcattt aaggtatctt gcaactcacg caaattcccc gaggtttcgt
    17041 ctaacaattg ctcacaactc ataattgctt cgtgccagtt ttggctaagc agttcggcta
    17101 ttcgttcacg gatttggtgc tgattttcgt ccataatccg ttgtgaaaga tcgatgctgt
    17161 caaagatttc cgccaccgaa tatttcaatg gggcaaacac attatttcgc caaaaacgtt
    17221 catcttgtgc ctgctctatt ccctcttccg ctgctactga agctcgctga atttcatctg
    17281 ccacaatcga aagttgaatc gataaacgca gggtagaaaa ttcccgctgg cgaacgtaat
    17341 aatttgaaat tcccacccct aacggcgtaa cacgataaat cgccaaacct tccgtaaatt
    17401 cgctactaaa acggtttaac aaccgttgtc tgactaaatc attaatcgca ttattggcac
    17461 gctgggtaaa agtggcatca gattgctcaa acgaatttga aacataacgg aaaagatcaa
    17521 taagatcact ttctaacaat tcaccatctg tctgttcttg gttataaata gagatagcaa
    17581 gtagaaacgc taagcggtcg gaagaaagcg aaagtgaaaa ttcacgatct ttcgtccaag
    17641 agatgagttc tggaatagtc tgtgccaatt cgttctgcat acgggcttaa tctttaattt
    17701 ctatacaaaa atggtattta ttgtaactga atgtaatcaa aatgggaatg gataaaacgc
    17761 agacaagaga aatgaacaat ataagcggtc aaatatgggt gattttttgc aaaaagtatt
    17821 ttaaaggtaa atgaatccga aaatttatta tcactacgct aatttatgat accataaata
    17881 gaaaagactg gatccaatag aatatcagac acagcccctt agatcatagt ctaaactcta
    17941 aggcttagag tgatttagct aactctttaa taaatgcttt aaattctttt ccgcatttgt
    18001 catgatttaa actatattct acaaatgctt tcatatagcc gactttataa ccacaatcaa
    18061 atgagcgccc tgtcatatga aaagcttcaa ccgtttcttg gtgaatcaac atatcaattg
    18121 catccgtcag ctggatttca tcacctacgc ctactggcgt tttttctaat aaattccaaa
    18181 tatttgagga aaatacataa cgtcctacta ccgctaaatt agaaggcgcc tcttcaatac
    18241 ttggtttttc aaccattttc gcaataggcg ctgccccccc gagagggata tctacaccat
    18301 ggcagtcagc aaccccatag ctacttacat tttccattgg aaccggcgca accataattt
    18361 ggctatatcc ggtttcttgg aaacgcttca ccatcgcagc taaattttcc gttttttggt
    18421 ttgctgtaaa ttcaccaagt aatacatctg gtaatacaac cgcaaacggt tcatcgccaa
    18481 ccactgttcg tccacataga accgcatgac ctaaaccttt ggcttgccct tggcgaacgt
    18541 gaattaaggt aacatctttt ggtacaatag aacgtacatc atctaataat tgacgtttta
    18601 ctcgtttctc cagcatcgtt tccaattcaa aagaggtatc aaaatggttt tcgatagcat
    18661 tctttgaaga gtgagtaacc aatacaattt ctttaaaacc tgcagccaca cattcattta
    18721 caatgtattg aataagaggt ttatctgcaa ggataagcat ttcttttgga atagctttgg
    18781 ttgcgggtaa catgcgcgtt cccaatccag caacaggaat aatgactttc atttggaatc
    18841 ctttgatgac ttaacatatt agatataact gaaataaacc gcttagacat aacaggcggt
    18901 caattttcag aaaattttgc attgcctgct tcgctgttat catctagcag ctttacagtt
    18961 aaaatgctta ttccgcattt tcctcttcta ctgaggcttt tattctttca taaatctctc
    19021 ggcggtgtac agagatttct tttggtgcat tcacacctaa tttaacttga tttcctcgga
    19081 tgctgagtac cgtaatctca acatcatctc ctacgagtaa aacttcccca atcttacgag
    19141 ttagaataag cataattgtc tcctaaaaag tgaaaaactc tataaatact taaatataga
    19201 gtaaagtttt aaaggcgagt tcagctcgcc ttgttattat ttttaaagtt ttgctgtaat
    19261 ccattcttga gcaagtgaca atgcttgcaa taaatgttgt ggttctgagc ctccggccat
    19321 tgccatatca ggacgtccgc cccctttacc tcccactttt tcagccattg caccaactaa
    19381 ttgaccagca ttgactttat cggttaaatc tttggtcaca cctacaatca aattgacttt
    19441 atcttccgcc gtagtcgcaa atacaacaac tgcactgcct aattgatttt ttaggtcatc
    19501 aaccatcgtt ctcaatgctt ttggttcaac attctctaat tgttgaatca ctacattcac
    19561 gccattgatt tgggtagctt gtttggctaa atctgaacct gcttgtgctg caagtttatc
    19621 ttttagctgt tgaagctctt tttctgtacg tttagccttc tcttgtaatt gaacaatttt
    19681 ctcaacgaat gaatgactgt ctgcttttaa caattcagca ctttgttgaa cggcttgttg
    19741 taaggtatgc aaccaagcaa tcgcattttc accagtgaca gcctctatac ggcgtacacc
    19801 tgctgctacc gcaccttcag aggtaatttt gaataagcca atatcaccgg tttgtttaac
    19861 gtgtgtgccg ccacaaagtt caatcgagaa atctgacatt tccactacac gcactacttc
    19921 gccatatttt tcaccaaata acgccattgc acctttcgct tttgcagatt caatatccat
    19981 ttcttcaatg attacctgaa tattttcacg cactttagca ttcacgatac gttcaacttc
    20041 ttcaagttgt gatttagtaa ttgcttccgg ctgagaaata tcaaaacgta aaatattttc
    20101 tgacactaat gagccttttt gagcaacgtg gtcgcctagc acttggcgta acgcagaatg
    20161 cagtaagtgg gtagcactgt ggtttaatgt aatagcatga cgacgctctc tatctacgat
    20221 cgtattaact ttgtcgccaa cagaaagtga gccggaagtt aattgcccaa tatgcccgaa
    20281 tacttgacca tatttttgcg tatctactac ctcaaaattg caaatttctg cggaaatttg
    20341 accgctatct cccacttgtc cgcccatttc tgcatagaat ggggttttat ctaaaatcac
    20401 taccgcattt tcacccgatt gaatagactc aaccgatttt ccattactaa ataacccaat
    20461 tacagtagca tcatttaagc tggtttcagt atatccacta aagcatgttt tactgtccac
    20521 tttaatcaca ttgttataat ctgtgccaaa gctactgctg gctttcgcac gctcacgttg
    20581 tgcggtcatt tctgcattga agccgtcttc gtcaatttta atatcacgtt cacggcaaac
    20641 atctgcggtt aaatctaacg ggaagccata agtatcataa agtttgaacg caacttcgcc
    20701 agataaggtg ttattttcaa ccttcgctaa tgcttcttct aataaattca atccacgctc
    20761 aagagtacgg gcaaattgct cttcttctgc tttgagtgtc ttttgaatat gcgcttgttt
    20821 ttgggtcagc acctcaccag cttgccccat taccgttgca agtgttggca ccaatttata
    20881 gaaaaaggct tcttttgcac ccaaaatatt accgtgacga accgcccggc ggataatacg
    20941 gcgtaataca tagcctcttc cttcatttga aggtaaaaca ccatcggcaa tcagatagga
    21001 acaagcacga atatggtctg ccactacacg taaagattta ttatctaaat ctttcacatt
    21061 aagcaaagta gcgacttctt taattaaagt ttggaaaata tccgtttcgt agttggagtt
    21121 tacgtgttgc attaccgcag tcatacgttc taaccccatc cctgtatcta cagaaggctt
    21181 aggcagtttt tccatcgtac cgtctgctaa acgattgaac tgcataaata caatattcca
    21241 tacctcaata tagcggtcgc catcttcttc tgggctaccc ggtaagccac cccaatgttc
    21301 aggcccgtga tcatagaaaa tctcggtaca agggccacaa gggccggtat cgcccatcgc
    21361 ccagaagtta tctgaagcat aaggtgcgcc tttattatcg ccaatgcgaa taatatgttc
    21421 cgatggtaca cctacttctt tatgccaaat atcgtaggct tcatcatcag tttcatacac
    21481 agttacatat aatttttctt ttggtaagcc aagccattgt ggagaagtta aaaactccca
    21541 accgaatttg attgcatctt gcttgaagta atcaccaaag ctgaaattcc ccatcatttc
    21601 aaaaaaagta tgatggcgag cggtataacc tacgttttct aaatcattgt gtttaccacc
    21661 tgcacgtaca caacgttgtg ccgtagtcgc tctgctataa gggcgttttt ctaacccaag
    21721 aaaaacatct ttgaattggt tcatgcccgc attggtaaat aataatgtcg gatcattctc
    21781 gggaacaaga gagctgcttg gaacaatcgt atgtccttta gtttggaaaa actctagaaa
    21841 agattgtctg atatctgaag ttgttttcat taaaaatcac tctttttgta aatagaagtt
    21901 aaacatttag ttatcttg
//
LOCUS       DANLXQ010000022        21204 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.022, whole genome shotgun sequence.
ACCESSION   DANLXQ010000022 DANLXQ010000000
VERSION     DANLXQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21204
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.022"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..167)
                     /locus_tag="PXD94_000666"
     CDS             complement(<1..167)
                     /locus_tag="PXD94_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6285558.1"
                     /translation="MKAQQLKNAILQLAIQGKLVPQDPNDEPASELLCKIQAEKDRLI
                     AEGKIKKNKKTA"
     gene            complement(373..2034)
                     /locus_tag="PXD94_000667"
     CDS             complement(373..2034)
                     /locus_tag="PXD94_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL6285559.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDLWHTYSAFA
                     NSNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKI
                     FQVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQK
                     DADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQN
                     SEGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYL
                     KIVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTK
                     DNKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQS
                     RNFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGENFSELQPIQKDCLLFIAFN
                     QGKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKS
                     QDDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            complement(2047..3516)
                     /locus_tag="PXD94_000668"
     CDS             complement(2047..3516)
                     /locus_tag="PXD94_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL6285560.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEMSDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            complement(3564..5915)
                     /locus_tag="PXD94_000669"
     CDS             complement(3564..5915)
                     /locus_tag="PXD94_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL6285561.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PXD94_000670"
     CDS             complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PXD94_000670"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL6285562.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPAEGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            complement(7045..7383)
                     /locus_tag="PXD94_000671"
     CDS             complement(7045..7383)
                     /locus_tag="PXD94_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL6285563.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            7676..8995
                     /locus_tag="PXD94_000672"
     CDS             7676..8995
                     /locus_tag="PXD94_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL6285564.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKTDTAQAPYVSLRSTKAPG
                     HTFTPIMKRPDAKVEDHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTA
                     SFNAVEAQENVEKEVVKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPK
                     TRRLLLVALLALALLVLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKRAEEQ
                     ARAEQEAAEAAKAQEQAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVA
                     VKPVENKPVVAQPAIKPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKT
                     AAKPVEKAPTKTEAVKAAPAAESANAVATKTMTVPKGVSLMQVFRDNNLNISDVNAMS
                     KVNNVVSNLKVGERVTVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            9113..9475
                     /locus_tag="PXD94_000673"
     CDS             9113..9475
                     /locus_tag="PXD94_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL6285565.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKESVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            9586..10623
                     /gene="waaF"
                     /locus_tag="PXD94_000674"
     CDS             9586..10623
                     /gene="waaF"
                     /locus_tag="PXD94_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL6285566.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCCPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYAILAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAEIMRLIEGGLIKIRKGEGAEGYHQSLIDIQPE
                     MVMDKLAELLV"
     gene            10690..11649
                     /gene="rfaC"
                     /locus_tag="PXD94_000675"
     CDS             10690..11649
                     /gene="rfaC"
                     /locus_tag="PXD94_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL6285567.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVECQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            11770..12144
                     /gene="rpsL"
                     /locus_tag="PXD94_000676"
     CDS             11770..12144
                     /gene="rpsL"
                     /locus_tag="PXD94_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL6285568.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            12275..12745
                     /gene="rpsG"
                     /locus_tag="PXD94_000677"
     CDS             12275..12745
                     /gene="rpsG"
                     /locus_tag="PXD94_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL6285569.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLTQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            12847..14949
                     /gene="fusA"
                     /locus_tag="PXD94_000678"
     CDS             12847..14949
                     /gene="fusA"
                     /locus_tag="PXD94_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL6285570.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
     gene            15009..16193
                     /gene="tuf"
                     /locus_tag="PXD94_000679"
     CDS             15009..16193
                     /gene="tuf"
                     /locus_tag="PXD94_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL6285571.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKHFGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALQALNGVAEWEEKILELANHLDTYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKLLDEGRAGENVG
                     ALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKEEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKI
                     IK"
     gene            16490..17788
                     /gene="tig"
                     /locus_tag="PXD94_000680"
     CDS             16490..17788
                     /gene="tig"
                     /locus_tag="PXD94_000680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL6285572.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
     gene            17904..18356
                     /locus_tag="PXD94_000681"
     CDS             17904..18356
                     /locus_tag="PXD94_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL6285573.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNDFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            18377..18877
                     /gene="secB"
                     /locus_tag="PXD94_000682"
     CDS             18377..18877
                     /gene="secB"
                     /locus_tag="PXD94_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL6285574.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            18984..19994
                     /gene="gpsA"
                     /locus_tag="PXD94_000683"
     CDS             18984..19994
                     /gene="gpsA"
                     /locus_tag="PXD94_000683"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL6285575.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRSHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            20010..20825
                     /gene="cysE"
                     /locus_tag="PXD94_000684"
     CDS             20010..20825
                     /gene="cysE"
                     /locus_tag="PXD94_000684"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL6285576.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            20874..>21204
                     /locus_tag="PXD94_000685"
     CDS             20874..>21204
                     /locus_tag="PXD94_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6285577.1"
                     /translation="MQLTFNDFQNYYNGKGDLTMFSFYGKKTWENGIYVGIDGSIGRA
                     NNKLSHQSQTIKLARNVYSLGLTVGKTLEVASFNLQPSFGMRYHHLTSANAQLENTKF
                     ETDRVDLL"
BASE COUNT         6397 a         4072 c         4565 g         6170 t
ORIGIN      
        1 gcagtttttt tgtttttttt gatctttcct tctgcaatca agcggtcttt ttctgcttgg
       61 attttgcaaa ggagttcgct tgccggttca tcatttggat cttgtggaac gagcttgcct
      121 tggattgcta gttgtaggat tgcgtttttt agctgttggg ctttcatttt ttattctcaa
      181 taatcggttg ttatttctta aagtatcttt gattacggtg ttttgggttt tctgggaatt
      241 gcatttcgat cacaccaagc gttagtgcag gttttaaata ctgcatacgg aaatgcttat
      301 catcttttaa cctgagtttt ttcataattt ctttgctctt catcggctca agcaacactg
      361 aaagtaagcg atttacttcg ggggtaattt gtttgctttg ttctggggta attgtttgac
      421 ttgcttcact ttgcttttct tcgggggggc gcccttactc tcactagctt tatcaagttg
      481 gtcgaaaggt ggattaaaat aatcatcttg tgatttctct gtgattatcc attctaaatt
      541 cactagggta tagagttgtt ttctcggact ccccgaactg gttagtaagc ctttttcttt
      601 cagtcgtgct agtgccaaag taatatttct gcgtggatat tggggcagtt tttgtgctaa
      661 ttctgcaaac attgctttac cttgattgaa ggcgataaag agtaagcaat ctttttgaat
      721 aggctgtaac tcagagaaat tttcgccaaa gcaagcggta aatttttcct caaaatctgc
      781 aatcatacct gattgaaaag ttaaggtgag tttagtgtaa tcaatttcac tttctaaaag
      841 cggtgaattg aaatttcggc tttgccaata tgaataaata tcccgaacgc cttgtccttg
      901 tcgctcacat agccctaaac ggtggaataa gttatgaata atagaattac ggcattccga
      961 tctttgccct gcaatagccg tgggaatatc aaccaacatt actcctgcat tttcaaaaat
     1021 cagtttgtta tctttggttt taatgacttt tagatggcga acattgttaa agtaatctgc
     1081 gtgagtaatt gcattgatta gagcttcccg tagcccgtca gtaatttcat tttcctcagt
     1141 acgggttaaa ttatctaatt tgaaatgttt atctttacca agctcttgaa taagtggcac
     1201 aattttcaag taaaattcaa agagattgcc ttcttcaaga tcaaaatcgg taatccgtaa
     1261 tgtatagcgt tttacatcac cagcttcttc gtaatattca aaaaagaagt ggggaaatat
     1321 gcttgtaatg gcatagtgct taccaaacat caataagcct gcattggtaa tgccctcgct
     1381 attctgtgct aaatcttttt tataagcccc aatttcttgc agcaatttct catcggataa
     1441 cgtaagtaat tttgatgtgc tgttatgggc tttcagcaga tttctgtatt tatccaatgt
     1501 gcttaaactg atatcatcaa tagaagcata aggcacaatt ctcccatcag catctttttg
     1561 gctatagctt gaaatgaaag aacgcagttc agaggaggtg agtttatgat cgcctgaatt
     1621 taaacgaaca taagaaagtc gataatcacc atttaaatgg ataggacggt ctaaaatatc
     1681 agctgctttc acataaattg caatcacgct tttattttca acttgaaaga ttttcacatc
     1741 attatccttt aataaggatt tactgacttt ttcactacga gattgggaaa ataattcatc
     1801 aatgacttta tcaggatcat taacaccaga aattagcagt tcaggaaaat gtccttgttc
     1861 attttccttt attcctaata aaataaagcc accattgctg tttgcaaaag ccgaataagt
     1921 atgccaaaga tcttttggca cactattttt tgcctcttta tattcaattt ctgccttttc
     1981 tcggataaat tgctgatgtg caatcatttt ttcgatatgt tcccaagtca gcattttatg
     2041 ctcctattac agtttaatgc ctaaaatatc ggtaatttct gctagaatct tatcaatatc
     2101 agcattgtaa gcggtgcgtt ttcgctgata atctgcaatt agttcatcag gcggaaggat
     2161 ttcctcttct tcgtgaggaa agccacataa atcgaggttg tattgacggt cggacatttc
     2221 ctttaatgta aaacttttcg ctttgtcaaa cccttcgact tcaatcactt ggcgatttcc
     2281 ccaccattca acaacttcgt tgaaatgttc caatttcatc ggtttagttt tggagaaatt
     2341 tttataaccc tgtggcatat caaggcgata aacccaagtt tcacgggttg gctcggtttt
     2401 atcaaaaaac agaatattag tcgtaatcga ggtataaggg gcaaagacac tatgcggtaa
     2461 gcggataatc gtatgcagat taaactcttc taatagcttt ttcttaatcg caactttggc
     2521 attatctgtg ccgaacaaaa atccgtcagg cagcaccacc gcagcccgtc cattctgttt
     2581 taggcggtac ataattaccg acataaacag atcagcggtt tcactgctct gtaaagccgt
     2641 agggaaattc tttttaattt gctcaatttc tgaacctccg taaggtggat tcatcaaaat
     2701 cacatcaaat ttatcatttt cggtgtaatc tttgaccgat ttttctagag cattatcgtg
     2761 atgtacattc ggattatcaa tatcgtgcaa caataaattg gtaatacaaa gcaggtgagg
     2821 aagggctttt ttctcaatgc catacacaga attattgaat aaagtgcggt cagaaagggt
     2881 ttgaatttga ctttccaata cctttaacgc cgaagttaaa aaaccacccg tcccacaggc
     2941 aaaatcggca atgcgttcgc caagtttcgg tttgattgct tgtaccataa aatcggttac
     3001 agcgcgaggg gtataaaatt cccctgcatt acctgcactt tgtaaacttt tgaggatatt
     3061 ttcataaata tcgccaaagg cgtgtcgttc ttggtaatgg gcaaaatcaa tttcatcaat
     3121 gatattgata acctgacgta ataacacccc atttttcata tagttgttat tatcttcaaa
     3181 agcagcacgg ataatccgtt ggttcatcgg cgtatcggct gaaataggca gattttttaa
     3241 agtcgggaaa agttcgttat tgacgaaaga gagtaattca tcaccagtca tcgctttgcc
     3301 gtctttttta tcttccgccc aattttgcca gcgtaaaaaa ttcggcaaaa ttgagtgata
     3361 ttcatcgttt tccagttccc actcttgttc tttagcatcg taaattttca agaataagat
     3421 ccagacgatc tgttcaatgc gttgggcatc accgttaatc cctgcatcgt tacgcattac
     3481 atcttgcagg cgttttacta tgttgttgag gctcattgtg atccttgttt atctgtaaaa
     3541 ttttgttgta aaataaccgc ttgttattcc gaataaatca tcttttctaa gccttttacc
     3601 gctttctggt agagttcttt accgccgaaa tttttaataa tagacactag acctccaaaa
     3661 cggctgaatt gcggtgtctt taaaatttcg gttttagtca gatcttgatt gccggatttg
     3721 acatattcat taagcagcaa ccctaaaact tgctggtttt cgggcgaata ctgctccaca
     3781 tagccacttt gtttcacttt ttctgctcgt tcggttttag tcagtggttt tttgccaaag
     3841 gagatgtggc ttaatacatc aaagtcttca acatcgtggc tgaattggcg tgtttggcga
     3901 agggctgcca gccattttgg atcggtgtaa aatttcgaga aaatttcacc gcttgttgtc
     3961 cattgctgtt ggaactgttc gtaactgccg aactcttttt gcatttcccg ttttgagaac
     4021 agatcaatat cttcagaaac cagctcgccc tgctcattca gatattggac tttctcgtct
     4081 aaaatgcgta atttgagtgt gccgaaatcg acaactaccg ggattttata tggctttagc
     4141 tcggggtctt taatgatgtc cttatcactg ggtttaggaa gcggttgttc gacaagtggc
     4201 ttattttccg gtacatctaa aatcaccacc ggctcaccat caaatgccgg atcagcaaac
     4261 aggcgagtaa cgcctcgaaa atctaaaatg gtgaaaaatt ctttgccgaa atcagggcga
     4321 agacgagtgc ctcgtccgat aatttgttta aattccgtca ttgattgaat gtttgcatca
     4381 agtacaacca gcttacaggt tttgctatct acacctgttg tcatcaattt agaggttacg
     4441 gcaatcacag ggtaatcttt cccttcattt gctacatcac aaaattcttc taaatcaacg
     4501 cctttgcctg attggtcgcc tgtaatttgg cagacatatt tggaattgtg tttcaacata
     4561 tcggcattga ggcgtgaaag ctctgccacc attcgctctg catggttgat gttggcacag
     4621 aagactattg ttttctgcaa gcggtcggtt ttgcgtaaaa attcgcaaat tctctccgca
     4681 accaagcggg tgcgatcatt tagcacaatg ttgtaatcga aatctttgac tgtatagagg
     4741 cgatcttcaa tctcattacc ttgatgatct tttaaacctt tggttggtcg ccagcctttt
     4801 aaatcacagt ccagatcaac acgaatcact cgataagggg caagaaagcc gtcttcaata
     4861 ccttgtttga ggctataggt ataaatcggc tcgccaaaat aggtaatgtt gctggcatca
     4921 tcggtttctt tgggggttgc agtcataccg atatgggtgg cgtccttgaa atacttcaaa
     4981 atagcacgcc agtcagaatc ttcttttgct gaaccacgat gacattcatc gatcacaata
     5041 agatcaaaga aagagggttg gaactgctta aacagttcgt tatcggtttc accgtcaagc
     5101 tgttgataaa gtgcaagata gacttcataa gcactgtcga gctttttatt gctgactttc
     5161 accattttgt ctttaaacgg tttgaagtct tgcatcattg gctgatcgac tagtacattg
     5221 cgatcggcaa gaaataaaat tttctttttc gcaccactgg tttttaaacg atggataatt
     5281 tggaaagccg tataggtttt acccgtgcct gttgccatta ccaataaaat cctctgctga
     5341 ccttttgcta cagcttcaac agtacggtta atcgcaattt cttggtagta acgaggctct
     5401 ttaaaggtgt cagtgctact ggtgtaataa ggctgttgaa taattttcag ctcttcggtg
     5461 gtatattgtt tcgcccaacg ttggtggagt tcgtcgggag taggaaatgc tgacatcgga
     5521 agggttcgtt gtacgccagt gataaaatcg tgttccacaa actcatcacc atttgagcta
     5581 tatgcaaaag ggacatcaag caatcgagcg tatgtcattg cttgctgtaa gccgtgtcgg
     5641 gaagagtgat ggttatcttt ggcctcgacc acggcaattt tttgatttgc ggaagtatag
     5701 agcagataat caacccgttt tgcttcgatg cgtttagttt gcgtcgtttt tggatcaaca
     5761 actacttgcc catctgtaaa tcgtttattg gttttaaatg tgtactcttc gccaatttgt
     5821 ctatccgacc atcctgcatt tctaattgca ggagagatta atcgagagcg aatttcttgc
     5881 tcgttcatct gctttaattc ttgaagtgtg tacatatttt tccttccgat aagagtttct
     5941 tatcggaaga ttatagcaaa cagtttaggt aaaattaact caggatttaa ttgataattc
     6001 tacccaactc ttcccccatt cgggtttgca ttcctgcaat aagtgcaggg ttgagatgta
     6061 ctttaccttt cgggaagagg ttaattacgg ttgagcctaa acggaaagct cccatttctt
     6121 cgcctttttg gagtttgatg gctttttcgc cttcggtttc gtaattccaa actaccactt
     6181 catcagcgcg aggcggatta attacgccag cccaaaccgt actcatactt gcggtaaccg
     6241 ttgcaccgac caaaatctgt agcattggtc cgaatgcggt gtcaaattca caaatcacac
     6301 gctcattgcg agcgaataag tttggaacgt gttcggctaa aaacggattg acggagaaca
     6361 attcgcccgg cacataaatc attttacgca atgttccatc gcacggcata tgtacacgat
     6421 ggtaatcagt tggcgataag taggtggtaa taaatgtgcc gtctttaaat tttgccgcca
     6481 tttcttcatc atttgccaat aaggtttcta gcgtaaaatg atgccctttt gcttgtaata
     6541 aacggttatc cgaaatttcg cctgattcgc tgactttacc ttctgccggt aagcaaagtg
     6601 tatttggatc ttgattaatc gggcgtgcgt tttcttttaa ttcacggata aaaaattcat
     6661 taaaagtagc ataatctgac ggctcggttt ttttagcttc agataaattg actttatact
     6721 gttttgcaaa cagtttgatg ataaggtgag taactacgcc ccatttttgc tctgctaacc
     6781 aacctgccgc acgggtaatt gccagttgtg gcagtaaata gtgcagagcg attttcacac
     6841 gttggaaata ggttggaact ggatattttt ttaattgcat tgcgttttcc ttttatttaa
     6901 aaacgagaat ttgcgtttaa ttgggggcga agtatagcaa atacaatgca aaatagataa
     6961 aaaatcttta aggtgttgaa gtggctacaa agaaaaaaac agataggggc tttgtaggaa
     7021 ttccacttca acaatttggt tgtcttattc taaaataata ttggctcgat ttttttctac
     7081 cgttgcttgc ccaattccgg agacttcagc aagttgctca atgtttttaa aattgccgtt
     7141 tttagtacgg taatcgacaa tcgcttgagc ttttttctgc ccaattccca ctagcttgtc
     7201 ttgcaattca gcagcactag cagtattgat atttaccgca ttagcgttgg tgatttgtgt
     7261 ttgctgtacg ggttgagtga ctgcattttg ttcaacaggc attgtagttg gcggtgtttg
     7321 ggcaaaagcc attgcattta agccaaacaa gaatgttaga ccaagttgtt tgattttctt
     7381 cataggattt ccttattagt tgttgaaagg tcgaaaggaa gattaagagg gaataaaata
     7441 gaactcaacc ggcaaaaata aatttttcga cacagatcga aaaaatagaa aatggctaaa
     7501 cttctagacg tgcatttggt ctgaaattca atgaaagtaa atagatttgc aaaaaattag
     7561 gcaaaaatga ccgctagttg cgtaaattta gttaaataaa ttcaaatagc ggctgaattg
     7621 ctttagaata tcgccatttt tatactcatt aacaacgcta ttatttggaa ttaatatgac
     7681 acaaaataat tttcgcaaag aaccggtttt tggtgaatct tcggttacct ctcaagacgc
     7741 tgaagcaacc aaaacagata cagctcaagc cccttatgtt tcgttgcgtt caaccaaagc
     7801 accggggcat acgtttaccc caattatgaa aagacctgat gctaaagtag aagatcatac
     7861 ggctgatgtc ttttcggcta agcaggcaaa agaattccaa tttactccaa gttcagaagc
     7921 tacagaagca gtagagccgg ataataccat tcattcggtt gaagaaagtg tatcaaccgc
     7981 atcttttaac gcggttgaag cacaagaaaa tgtggaaaaa gaagtggtta aacctgcacc
     8041 cgagagtttc actactgagc gtattattcc aaatgcggca acagctgccg cagcagccac
     8101 tgcggcagct gcagccacta aaaccaaaga gattaaagct aaaatgccac ctaaaacccg
     8161 ccgtttatta ttagtggcat tgttggcgtt agcattattg gtactgtttt tcttgttaaa
     8221 accaagtacg ccggaaactg tcgaagagct acaatctcag caaggtggta gcttgccgat
     8281 tgaattccga ccggttgatg aagctgaagc gaaacgtgcg gaagaacaag ctcgtgcaga
     8341 acaagaagcc gctgaagctg caaaagctca agaacaagct caagccgctc aacaagagtc
     8401 tgtaacagtt gctccagccc aaaccgagca aacaatggct aaaccgtcac aagatagcca
     8461 gtcgcaaaca caatcacaag cagtagttga gcaacctgtt gcggtgaaac cggtggaaaa
     8521 taaaccggtt gttgcacaac cggcgattaa gcctaaaacg gaaaatagcg tggtgttcca
     8581 acctgaaaca acggtaaaac aacagcctaa agcccctgct aaaactgaag tgaaaaccga
     8641 acgtgctaca ctggcagcaa aagaaacggc aaaaacagca gctaaaccag ttgagaaagc
     8701 tccgacaaaa actgaagcgg taaaagcagc accggctgcc gagtcggcaa atgcggtagc
     8761 aaccaaaaca atgactgtgc caaaaggtgt atcactaatg caagtgttcc gtgataacaa
     8821 tttaaatatc tcagacgtta atgcgatgag caaagtcaat aacgtggtga gtaatttaaa
     8881 agtaggcgag cgagtaaccg ttcgtttaga taaaaataac cgtgtggttg aaatgagcat
     8941 tggctcaggc ggcaaattta cccgtcaagc aaacggtagc tatactttta aataaataaa
     9001 atgaagaaaa agagcaacgc tacaaacgtt gctcttttat ttaaaaagga gataaaggat
     9061 gaatttattt tgcccctctt acaaacttat ctacttaacg ctaagctcag cgatgttagt
     9121 tgcttgtagt acagtggtta cccctgttga gcaaattcat caagtacaaa aggaaaaaga
     9181 gtcagtaaag aaagtgacta aactgatagc acaagcacct aaagtgagtt tatatcgttg
     9241 tgagaatgat aagaaggttg aagttgaacg ccagggacga acaaaaaaag cgttaaaaaa
     9301 ggaaatagtc acagtgagtt atcaagggac aacgcatcag ctttcacctt ctgtaacgag
     9361 agatggtaaa aaatacacta atatccgttg gacttggcat gaaacccgta gtggtaaagc
     9421 atttttgtat aacaacacta aaaaaacgtt agcggcaaat tgcataaagc agtaacaagc
     9481 ggacacaaaa agtatcgctt tttgaacgtt ggctttgcca acggcagcga agctgtgaac
     9541 aatccttaca aggattgtga ataaattttt aagagaattt tgcaaatgaa tattttagtt
     9601 ataggaccgt cttgggtggg tgatatgatg atgtcgcacg cactttatca acagcttaaa
     9661 atacagtatc cgaattgcca aattgatgta atggcacccg attggtgttg tccgctttta
     9721 gcccgtatgc ctgaggtgcg taatgcgatt tcaatgccga ttggacacgg ttcatttcgt
     9781 ttgtgtgaac gctaccgact cggaaaatcc ctgagaaatc aatatgatat ggcgattgta
     9841 ttgcctaatt cattaaagtc tgcctttatt ccactgtttg cgaaaattgc gagacgtcgt
     9901 ggttggaaag gggaaagtag atattttttc ttaaatgatc ttcgcagtaa taaaaacgac
     9961 tacccaatga tggtgcaacg ctatgtagcc ttggcgtttg aaaaggctac tgttccaacc
    10021 gcacaacagt tgccgattgc ttatccatac ctcacaactg atagcgaaca aattgagcag
    10081 accaaagcga aatttgaaaa acagttggct tatgcggaaa accgtcctgc catcggcttt
    10141 tgtccagggg ctgaatttgg accggcaaaa cgttggccgc attatcatta tgcaatattg
    10201 gcaaaaatgt tgattgagca aggctattcg gtacggttat ttggctcgaa aaaagatgaa
    10261 gccgccggtg agcaaattcg cttggcatta cctgaaaatt tacaacgcta ttgcctgaat
    10321 ttagccgggc aaaccgattt aaaccaagcg gtagatttga ttgcagattg taatgcagtc
    10381 gtgagcaatg actccggctt aatgcacatt gccgcagcac taaataaacc gctggtcgcc
    10441 ttatatggac cgaccagccc acaatatacg ccgccgttat cccaaaatgc cgagatcatg
    10501 cgcttaattg aaggcggttt gattaaaatc cgcaaaggcg aaggggcaga gggctatcac
    10561 caaagtttaa ttgatattca gcctgaaatg gtgatggaca aattagctga actgttagtt
    10621 taaatggata acaagcggtt gaatttgcca attttttgca aaaaaagacc gcttgtttgt
    10681 ttataaaaga tgaaaatttt aatcgttaaa acctcatcaa tgggagatgt attacatacg
    10741 cttccagcca taaccgatat gcaaaatgct attcccgatt taaaagtaga ttgggtggtg
    10801 gaggagaatt ttaccgaaat tcctgcgtgg cattcagcgg taaataaagt aattccggtg
    10861 gctattcgcc gctggcggaa aaacctttgc caaagacaga cttgggtaga atgccagcag
    10921 tttttaaaac agctaaaaag tgagcagtat gatgcggtaa ttgatgctca agggctgatt
    10981 aaaagtgcgg tgttagtaac tcgccaagcc aaaggcaaaa aatttggcta tgataaaaac
    11041 tctgcccgag aagggttaag tagtctgttt tacgatgaaa cgtttaatat cccctaccaa
    11101 caacacgctg tagaacgcat cagaaaatta tgtgcaaaat cattaggtta tgctttgcca
    11161 accgaacggg gcgattattg cattgcacaa cattttgcaa aaaatcctca aaaaacgacc
    11221 gcttacatta tgctgattca cgccactacc cgagcggata aacattggca agaagcctat
    11281 tgggcggagt tgattcaagc gttggataag cacaatctgg cagtcagatt gccttgggga
    11341 aaccaagccg aaaaagaacg ggcggagcgt ttagcgagtg tggcgaataa tgctaaggta
    11401 ttgccaaaaa tgagcttaac cgatattgct actcaacttg cgggagcagc ggcagtggtg
    11461 tcggtggata ccggcttgag ccatttggcg gctgcgttag ataagccaaa tgtgatttta
    11521 tatggggcaa ccgatccaaa acttatcggg gcttacgggc aaaatcagtt ttatctaaaa
    11581 gcagataaaa tggaaaatat tacctcttca caagtgctag aaaaattact tccattgatt
    11641 aacaaatgat cattgttgtt ctcttactgt gcttgacttt tatccacgtt caatttagaa
    11701 ttcaccgtcc tttcgtgtga aaggatgttt ttaattttat ttttaataat tttaactgga
    11761 gcttttttaa tggcaactat caaccagcta gtacgcaaac cgcgtgtgaa aaaggttgta
    11821 aaaagtaacg ttcctgcatt agaggcttgc ccgcagaaac gtggcgtgtg tactcgtgta
    11881 tacactacta cacctaaaaa accgaactca gcattacgta aagtatgtcg tattcgttta
    11941 acaaatggct ttgaagtaac ttcatacatc ggtggtgaag gtcacaacct tcaagaacac
    12001 agtgttgtat taatccgtgg tggtcgtgtt aaagacttac cgggtgttcg ttaccatact
    12061 gtacgtggtg cacttgactg tgcaggcgtt aaagatcgta aacaaggtcg ttctaaatac
    12121 ggcgttaaac gtcctaaagc ttaatggttc tccgttgagt aaggccaaac gtctaaattt
    12181 taatatttaa accataaact ccagtcaaga tgccttaagg cagattagat gagttttgga
    12241 tatcctgaag atttaatata cggagaaatt tccaatgcca cgtcgtcgta gtattgaacc
    12301 acgcaaaatt cttccagatc cgaagttcgg ttcagaatta cttgcaaaat ttattaacgt
    12361 gttaatggta gatggtaaaa aatctactgc agaaaaaatc gtttacggtg cattagaaac
    12421 tttaacacaa cgttctggta aagaagcgtt agaagctttc gaaatcgcat tagaaaacgt
    12481 acgtccaact gttgaggtta aatctcgccg tgtgggtggt tctacttacc aagttcctgt
    12541 agaagttcgc ccaacccgtc gtaatgcttt agcaatgcgt tggatcgtag aagccgctcg
    12601 taaacgtggt gacaaatcaa tggcattacg tttagctaac gaattatcag atgcagcaga
    12661 caacaaaggt tcagcagtga agaaacgtga agacgttcac cgtatggctg aagctaacaa
    12721 agcgtttgct cacttccgtt ggtaatcgtt taagcaaact tatcaaaggc ttcatctcag
    12781 tttgcggtga agccttaccc ttattaagaa ttttaattta aaccccaaac aaggtaataa
    12841 ataaaaatgg ctcgtacaac ccctatttca ctttaccgta acatcggtat cagtgcgcac
    12901 atcgatgcgg gtaaaacaac tactacagag cgtatcttgt tctatacagg tgtaagtcac
    12961 aaaatcggtg aagttcacga tggtgctgca acaatggact ggatggaaca agaacaagag
    13021 cgtggtatca cgatcacatc tgctgcaaca acagcattct ggtctggtat gtcacaacaa
    13081 tatccacaac accgtatcaa cgttattgat actccgggac acgttgactt tactatcgaa
    13141 gtagaacgtt caatgcgtgt acttgatggt gcggtgatgg tttattgtgc agttggtggt
    13201 gttcaaccac aatctgaaac tgtatggcgt caagctaaca aatataaagt gccacgtatc
    13261 gcgttcgtaa acaaaatgga ccgtacaggt gcaaacttct tacgtgttgt tgagcaaatc
    13321 aaaacgcgtt taggtggtaa ctcagttgca ttacaacttc caatcggtgc agaagacagc
    13381 ttcaaaggtg ttgttgattt aatcaaaatg aaagcaatca actggaacga agctgatcaa
    13441 ggtatgacct tcacttatga agatattccg gcagatatgc ttgaagcttg tgaagaacac
    13501 cgtgcaatgc ttgttgaagc agcagctgaa gcttctgaag aattaatgga aaaattcttc
    13561 tctggtgaag aattaactga agatgaaatc aaagcagcat tacgtcaacg tgtattagca
    13621 ggtgaagtaa tccctgtttg ctgtggttct gcatttaaaa acaaaggtgt acaagcaatg
    13681 cttgatgcgg taatcgatta cttaccggca ccaactgata ttcctgcaat taaaggtatc
    13741 aatgaagatg aaactgaagg tgagcgtcac gcaagcgacg atgagccatt tgcttcatta
    13801 gcattcaaaa ttgcaacaga cccattcgta ggtaacttaa ccttcttccg tgtttactct
    13861 ggtgtaatta actctggtga tacagtttac aactcagtaa aacaaaaacg tgaacgtttt
    13921 ggtcgtatcg tacagatgca cgcaaacaaa cgtgaagaga tcaaagaagt tcgtgcgggc
    13981 gatatcgctg cagcaatcgg tttaaaagat gttggtacag gtgacacatt atgtgcaatc
    14041 gatgcaccaa tcattcttga gcgtatggaa ttccctgagc cggtaatctc tgttgcggtt
    14101 gaaccaaaaa ccaaagctga ccaagagaaa atgggcttag ctttaggtcg tttagcacaa
    14161 gaagaccctt cattccgtgt tcacacagat gaagaatctg gtgaaacgat catttcaggt
    14221 atgggtgagt tacacttaga catcatcgtt gaccgtatga aacgtgagtt taaagtggaa
    14281 gcaaatatcg gtaaaccaca agtatcttac cgtgaaacta tccgtactcg tgttaatgat
    14341 gttgaaggta aacacgcaaa acaatctggt ggtcgtggtc aatatggtca cgttgtgatc
    14401 gacttatacc cattagatgc tgaaggtccg ggttatgagt ttgtaaacga gatcaaaggt
    14461 ggtgtaatcc ctggtgaata cattcctgcg gttgataaag gtatccaaga acagcttaaa
    14521 tctggtccgt tagcaggtta cccggtagta gatttaggtg tgcgtttaca cttcggttca
    14581 taccacgatg ttgactcatc tgaacttgcg tttaaacttg ctgcatcatt agcatttaaa
    14641 gcggcgttcg caaaagcaag cccggtatta cttgagccaa tcatgaaagt ggaagttgaa
    14701 actccaccgg attacgtggg tgatgtaatc ggtgacttaa gccgtcgtcg tgcaatggtt
    14761 aacggtcaag aagcgaacga gttcgttgtt aagatcaatg ctgaagttcc actttcagaa
    14821 atgtttggtt atgcaacaga cttacgttca caaactcaag gtcgtgcatc atactctatg
    14881 gaacctttaa aatatgctga agcaccgaaa aacgttgctg atgcaattat tgaagctcgt
    14941 agaaaataat ttgtttaacc tgagccatag caatagggct atggctcatt ttttaggaag
    15001 atattaaagt gtctaaagaa aaatttgaac gtacaaaacc gcacgttaat gtgggtacaa
    15061 tcggccacgt tgaccatggt aaaacaactt taacagcagc aatcactacc gtattagcaa
    15121 aacacttcgg tggtgcagct cgtgcatttg accaaatcga taacgcgcca gaagaaaaag
    15181 cgcgtggtat caccatcaac acttcacacg ttgagtacga tacagcaact cgtcactacg
    15241 cacacgttga ctgcccggga cacgcggact atgttaaaaa catgattacc ggtgcggcac
    15301 aaatggacgg cgctatctta gtagtagcag caactgacgg tccaatgcca caaactcgtg
    15361 agcacatctt attaggtcgc caagtaggtg taccatacat catcgtattc ttaaacaaat
    15421 gcgacatggt agatgacgaa gagttattag aattagttga gatggaagtg cgtgaacttc
    15481 tttcacaata cgacttccca ggtgatgaca ctccaatcgt acgtggttca gcgttacaag
    15541 cgttaaacgg cgtagcagag tgggaagaga aaatccttga attagcaaac cacttagaca
    15601 catacattcc agagcctgag cgtgcgattg ataaaccatt cttattacca atcgaagacg
    15661 tattctcaat ttcaggtcgt ggtacagtag taacaggtcg tgttgagcgt ggtatcatcc
    15721 gcactggtga tgaagttgaa atcgtaggta tcaaagatac agcgaaaaca accgtaacag
    15781 gtgttgagat gttccgtaaa ttattagacg aaggtcgtgc gggtgagaac gtaggtgcat
    15841 tattacgtgg tactaaacgt gaagaaatcg aacgtggtca agtattagcg aaaccgggtt
    15901 caatcactcc acacacagac ttcgaatcag aagtttacgt attatcaaaa gaagaaggtg
    15961 gtcgtcacac tccattcttc aaaggttacc gtccacagtt ctacttccgt acaacggacg
    16021 taacaggtac aatcgaatta ccggaaggtg tagagatggt aatgccgggc gacaacatca
    16081 aaatgactgt tagcctaatt cacccaatcg cgatggacga aggtttacgt tttgcgattc
    16141 gtgaaggtgg tcgtacagta ggtgcgggcg ttgtagctaa aatcatcaaa taattaagat
    16201 tgtttgataa attgaaaagc taaataaaaa ctagaaagag gcatatcgaa aggtatgcct
    16261 cttttgcttt tatgccgatt aagctagcca tatcatatca tttcatttgc aaaaatcggt
    16321 gaaaaaagac cgcttgtagg caagcccatt gacaagcagt cagattttag ggatttttta
    16381 caaaactgtt tatttgcgaa tgagtttctt ctcgacagtt tcttgataga acgctacaat
    16441 aaccgtctat ttgtatatct tttttatatc cctaaaccga ggtttaagaa tgtcattttc
    16501 aattgaaaca ttagaaggct taaaacgccg tgttactatt aacgtaccag ctgataaaat
    16561 cgaagcagca tacaaagaac aattaaaagg ctatgcgaaa aatgctcgtg tagatggctt
    16621 ccgtaaaggt aaagtgccac actcaattat cgaacaacgt tttggtattg cagcccgtca
    16681 agatgtgtta tctgatgaaa tgcaacgtgc attctttgaa gccgtcattg ctgaaaaaat
    16741 caacttggca ggtcgcccta ctttcacgcc aaacaactac caaccaggtc aagacttcag
    16801 ctttactgcg gaatttgaag tattcccaga agtggaatta aaaggtttag agaatatcga
    16861 agtagaaaaa ccggttgttg aaatcgcaga cgctgattta gacaaaatgg tagatgtatt
    16921 acgcaaacaa caagcgactt ggtcagaaag ccaagaggct gcaaaagccg aatctcgtgt
    16981 aactattgat ttcgttggtt cagtagatgg tgaagaattc gaaggcggta aagcatcaga
    17041 cttcgtgtta tttatggggc aaggtcgtat gatcccgggg tttgaagacg gaatcgtagg
    17101 tcgtaaagcg ggtgagcaat tcgacattga tgtgactttc ccagaagaat accacgctga
    17161 aaacttaaaa ggcaaagcag cgaaatttgc aattaccttg aaaaaagtag aagagatggt
    17221 attaccggaa ttaaccgaag agtttgtgaa aaaattcggt aatgcaaaat cagtggaaga
    17281 attacgcgca gaaattaaga aaaatatgca acgtgaactt aaaaatgcag tcacttcacg
    17341 tgtgaaaact caagtaatca acggcttaat tgctcaaaat gatgttgaag tgccggctgc
    17401 tgcggttgaa gaagaaatca acgtattacg ccaacaagcg gtgcaacgtt ttggtggtaa
    17461 gcctgaaatg gcagctcaat taccggctga attatttgaa gcagaagcta aacgccgtgt
    17521 tcaagtgggc ttattattat cttcaatcat cggttctaac gaattaaaag tagatgaaaa
    17581 acgtgttgaa gagatgatcg cagatatcgc ttcagcttac gaacaaccgg ctgaagtggt
    17641 tgctcactat gcgaaaaacc gtcaattaac tgaaaatatc cgcaatgtgg tattagaaga
    17701 gcaagcggtt gatgcagtgc tagcgaaagc gaaagtgact gaaaaaacaa tgtcatttga
    17761 cgaagtaatg tcacaacaag ctcaataatt tccttataag gtgggctttt gctcaccttt
    17821 tattactttt acaagcggtt aaatttagtg ataaatttgc atcaaaacga ccgcttattt
    17881 tttgatgtta aggaaattga attatggaac aactaacttt tgctcaacaa ttagagcaat
    17941 tttttgccaa tcataccttg atggtgtttg cttgggttgc attattgatt gcggtaattt
    18001 ttagctttta taaaagtgca acgcaaaaat ataaaattgt cgataacgca caggctacac
    18061 agctgattaa taaagaagag gcagtagtgg ttgatttacg ttctgatgaa gagttccgtg
    18121 ccggtcatat tattgatagc attcacgtgt taccgtcaga cattaaagca gctaaaatca
    18181 atcagattga taaatacaaa gctcgcccgg taattgtagt agacactaat gacttcactt
    18241 caggtggtgt tgcggcagat cttgctaaac aaggctttag cagagtttat gtattaaaag
    18301 aagggattgg cggctggcgt gcagcaagcc taccaactat taaaaaacat aaataatcta
    18361 ttattaagga aattttatgt cagaagaaaa tcaagttgca tcaacagaag aagctacaca
    18421 aacccctttt gagcttcaaa ttcaacgcat ttatgtaaaa gatgtttcat ttgaagcacc
    18481 aaatttaccg actattttcc aacaagagtg gaaaccacaa ttaggctttg agttagatac
    18541 tgaaaccaaa gaaattggtg aagatttata cgaagttacc ttacacatta cggtgcaaac
    18601 tacgttagaa gacagcaatg atgtcgcttt catttgtgaa gtaaaacaag caggtgtgtt
    18661 tactattaaa ggtttagaag gcattcaact tgctcactgc ttagcttcac aatgtccgaa
    18721 tgtgctttat ccttatgctc gtgagctaat ctcaagcctt gtgaatcgtg gtacattccc
    18781 ggcattaaat ctctcaccgg taaactttga tgcgttattt atggattatc tccaacgcca
    18841 agaagaaagt gcagaagagc cggctttagc gaactaagca ttttagatag aaaataggta
    18901 tgcaaggcat acctattttt ttacaagcgg ttattttttc tactattttt tgcaattttc
    18961 taaataagcc gaacagagga aatatgagtg aaatctattc tgccccaatc acggtattag
    19021 gtgcaggttc ttatggaaca gctttagcca ttgccctttc tcgaaatggt cacaaaacct
    19081 atttatgggg gcataatccc gacaaaatgg ccgttttggc atcagagcga atgaaccaag
    19141 attttctgcc tgatattgta ttcccggaag ccttagaaat tgtcgcagat cttgctacag
    19201 cggtacaaaa atcgaaagat ctgctgattg tggtgccaag tcatgtattc tctgaagtat
    19261 tggctcaaat caagcctttt ttgtcccaag atcaccgtat aatgtgggca accaaagggc
    19321 tggagcgaga taccgggcgt ttgttgcaaa cggtagctaa ggaaattttg ggggaaattt
    19381 acccgcttgc ggtgctttcc ggtccgactt ttgccaaaga actggcggca ggcttgccaa
    19441 cagcgatttc gcttgcctca actgatcctg tttttgccga tcaaatgcaa aaacggagtc
    19501 actgttccaa agcgttccga gtttacctca acaatgatat tatcggtgtg caactcggtg
    19561 gggctattaa aaatgtgatt gcaattggtg ccggtatttc agacggaatg ggattcgggg
    19621 cgaatgctcg taccgccttg attactcgag ggctggcgga gattagccgt ttgggtgttt
    19681 cgctcggggc tgatccgaaa acctttaccg gaatggcggg gcttggagat ttagtgctga
    19741 cgtgtaccga taaccaatcc cgaaaccgcc gatttggttt ggcgttaggg caaggaaaat
    19801 cggcagaagt cgcgatagcg gaaatcggtc aagtggtaga aggattttat aataccaaag
    19861 aagcctattt gctcgctcaa acacagggcg ttgaaatgcc gattgtagaa caaatttacc
    19921 aaatgttatt ttgtggcaaa caggcttcgg atgtggtgaa aaccttgttg gggcgtgaac
    19981 gaaaaggcga ataaagaaag gagaataaaa tgcagcagag tgaaattaat cagatttggc
    20041 aagcaattcg agatgaagcc aaagagctgg cgaattgtga acctatgtta gcaagttttt
    20101 tccacgctac cattttaaaa caccataatc taggcgatgc gttaagttat attttggcga
    20161 ataagttaga aaacccgatt atgccggcaa tcgccttaaa agaaattatt gaggaagctt
    20221 atcgagcaga gccacaaatt attgctagtg cagcttgtga tattaatgcg gtcagaacta
    20281 gagatcctgc ggtggacaaa tggagtacac ctctgctgta cttaaaaggc ttccacgctt
    20341 tacaaagcta tcgggtgacg cattatcttt ggaatcaagg caggaaagca ttagcggttt
    20401 atttacaaaa tgaaatttct gttgcctttg atgtggatat tcatcctgcg gcaaaggtcg
    20461 gttgcggaat tatgtttgac cacgccaccg gtattgtggt gggtgaaacc tctgtgatcg
    20521 aaaatgatgt ctcgatttta caaggtgtaa cactaggcgg tacagggaaa gaaaatggcg
    20581 accgccaccc gaaaatccgt gaaggcgtga tgattggagc cggtgcaaaa attctcggta
    20641 atattgaaat cggacgctat tctaaaatcg gggcaaattc agtggtgcta caacctgtgc
    20701 ctgatcacgc cacagcagca ggtgtaccgg ctcgcattat cggcaaatcc tctgaacaaa
    20761 aaccggcttt tgatatgaat caatactttg aagatgagca aggtttgttt ggtgatggga
    20821 tttaggtaag agttattcct ttaatttatg atttagacat tgctaagtac aagttgcaat
    20881 taacctttaa tgattttcag aactactata atggtaaagg ggatttaact atgttttctt
    20941 tttatggtaa gaaaacatgg gaaaatggca tttatgtagg gattgatggt agcattgggc
    21001 gtgcgaataa caaattaagc catcaaagcc agacgataaa actagccaga aatgtatatt
    21061 ctttaggatt aacggtaggt aaaacgttgg aagttgcaag ttttaaccta caaccgtcat
    21121 ttggtatgcg ctatcatcat ttaacatctg caaatgccca attagagaac acaaaatttg
    21181 agactgatag ggtagatctt ttgg
//
LOCUS       DANLXQ010000023        21054 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.023, whole genome shotgun sequence.
ACCESSION   DANLXQ010000023 DANLXQ010000000
VERSION     DANLXQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21054
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.023"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            67..543
                     /gene="greA"
                     /locus_tag="PXD94_000686"
     CDS             67..543
                     /gene="greA"
                     /locus_tag="PXD94_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL6285578.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIVGDDEANIKEGLISVNSPIARGLIGKEVDDSVSITTPGGKVEFDIVGVEYI"
     gene            complement(599..1015)
                     /gene="ndk"
                     /locus_tag="PXD94_000687"
     CDS             complement(599..1015)
                     /gene="ndk"
                     /locus_tag="PXD94_000687"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL6285579.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAISHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(1025..3103)
                     /gene="metG"
                     /locus_tag="PXD94_000688"
     CDS             complement(1025..3103)
                     /gene="metG"
                     /locus_tag="PXD94_000688"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL6285580.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYIASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNSDIVNKLVNLA
                     SRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIANFYENREFNKAIRAIMELTDKA
                     NKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLMSYLKPVLPKLAERAEAFLQSE
                     LRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDETKALFAEANKKTENQTKGTQKV
                     EQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLNCEAVPKSDKMLRFELDLGDHQ
                     RQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGMSEGMILSAGTGGANLFLLDAD
                     SGVQPGMQVK"
     gene            3315..4286
                     /gene="menC"
                     /locus_tag="PXD94_000689"
     CDS             3315..4286
                     /gene="menC"
                     /locus_tag="PXD94_000689"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL6285581.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            4531..5232
                     /gene="rsuA"
                     /locus_tag="PXD94_000690"
     CDS             4531..5232
                     /gene="rsuA"
                     /locus_tag="PXD94_000690"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL6285582.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
     gene            5222..6403
                     /locus_tag="PXD94_000691"
     CDS             5222..6403
                     /locus_tag="PXD94_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL6285583.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHRPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(6543..7862)
                     /gene="rlmD"
                     /locus_tag="PXD94_000692"
     CDS             complement(6543..7862)
                     /gene="rlmD"
                     /locus_tag="PXD94_000692"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL6285584.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTIALFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            7947..9272
                     /locus_tag="PXD94_000693"
     CDS             7947..9272
                     /locus_tag="PXD94_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL6285585.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDNNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            9393..9692
                     /locus_tag="PXD94_000694"
     CDS             9393..9692
                     /locus_tag="PXD94_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285586.1"
                     /translation="MRFLPSEIYQPRGELVKADRQGNGEFEVEYRVSGNDVRGLAKSA
                     IAHAKRKGFHLVESDIHRDDADLKFKRGDQELDIEIEVKGRNRIEYKADLDLDKN"
     gene            9853..12765
                     /gene="rapA"
                     /locus_tag="PXD94_000695"
     CDS             9853..12765
                     /gene="rapA"
                     /locus_tag="PXD94_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL6285587.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKPELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
     gene            12892..13188
                     /locus_tag="PXD94_000696"
     CDS             12892..13188
                     /locus_tag="PXD94_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL6285588.1"
                     /translation="MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKV
                     VLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            13172..13414
                     /locus_tag="PXD94_000697"
     CDS             13172..13414
                     /locus_tag="PXD94_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL6285589.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR"
     gene            13426..14094
                     /gene="rluA"
                     /locus_tag="PXD94_000698"
     CDS             13426..14094
                     /gene="rluA"
                     /locus_tag="PXD94_000698"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL6285590.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(14072..14647)
                     /gene="ampD"
                     /locus_tag="PXD94_000699"
     CDS             complement(14072..14647)
                     /gene="ampD"
                     /locus_tag="PXD94_000699"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL6285591.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVIHYISLPPE
                     EFGGNFIDLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFNDMAWHAGV
                     SCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDERITGHCH
                     IAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            14707..15549
                     /locus_tag="PXD94_000700"
     CDS             14707..15549
                     /locus_tag="PXD94_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL6285592.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSALPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            15672..16313
                     /gene="pyrE"
                     /locus_tag="PXD94_000701"
     CDS             15672..16313
                     /gene="pyrE"
                     /locus_tag="PXD94_000701"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL6285593.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYGCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            16487..17576
                     /locus_tag="PXD94_000702"
                     /pseudo
     CDS             16487..17576
                     /locus_tag="PXD94_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(17641..18831)
                     /gene="metC"
                     /locus_tag="PXD94_000703"
     CDS             complement(17641..18831)
                     /gene="metC"
                     /locus_tag="PXD94_000703"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL6285594.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     NHVLMTGAAYEPTQEFCNALLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSSIAVAN
                     WLAQRPEVKAVYHPALESCPGHEFFKRDFLGSSGLFSFELCEKLSNDKLANFLEHFEL
                     FSMAYSWGGFESLILANQPEEIAKIRPAIERKLTGTLIRIHVGLEAVEDLIADLEQGF
                     NRIR"
     gene            complement(18890..19429)
                     /gene="dsbB"
                     /locus_tag="PXD94_000704"
     CDS             complement(18890..19429)
                     /gene="dsbB"
                     /locus_tag="PXD94_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL6285595.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLVLSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(19492..21033)
                     /gene="nhaB"
                     /locus_tag="PXD94_000705"
     CDS             complement(19492..21033)
                     /gene="nhaB"
                     /locus_tag="PXD94_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL6285596.1"
                     /translation="MSSIDALFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGW
                     FLVAEFIFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMV
                     AGIYFMKQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGF
                     YGVYHKVASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTM
                     VGEPQNLIIASQAEWGFIEFLLRALPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKV
                     WGVLARYSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAF
                     CGITDEHTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLF
                     YIFNGLLSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPN
                     GQAAFLFLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWG
                     WFITR"
BASE COUNT         6146 a         4179 c         4788 g         5941 t
ORIGIN      
        1 aaattgaata atagcccttt cgttttaatt cgtaaaccga taatttttag taaggaaata
       61 gatttaatga aacaaattcc tatgacggtg cgtggtgcag agcaactgcg tgaagaattg
      121 gattttttaa aaaatgtgcg tcgcccacaa attattgatg cgattgcaga agcccgtgaa
      181 cacggtgatt taaaagagaa cgcggagtat cacgcagccc gtgaacaaca aggtttctgt
      241 gaggggcgta ttcaagaaat tgaaggcaaa ttaggcaatg ctcaaattat tgacgtcact
      301 aaaatggcaa acaacggcaa agtgattttt ggggcaacgg tgcagttagt gaatgctgat
      361 acagatgaag aagtcactta ccgcattgtg ggtgatgatg aagccaatat taaagagggc
      421 ttaatttcag ttaattcccc gattgctcgt ggtttaatcg gcaaagaggt ggacgattca
      481 gtcagcatta ccacaccggg tggcaaagtt gagtttgaca ttgttggcgt agaatatatt
      541 taatctacgc tagggcgaat tacaatagtg gggcgaaaaa attttcgccc ctactttatt
      601 aaacaatctc actcggtaca aagaagtagg caatttcacg ttttgccgac tcttcggaat
      661 ctgaaccgtg taccgaattt tcacgcatac ttaaggcata taaatcgcga atggtgcctt
      721 tttcacggtt atccggattg gttgccccca ttaatgcacg ataatggcta attgcatttt
      781 ccccttccag tacagaaacg acaatagatc ctgatgtcat aaattccact aacggctcaa
      841 agaaatcttt gcctttgtgt tccgcataaa atccttccgc ttgctcacgg gtaagatgta
      901 ctttttttag agctttgata gtcagcccgt tttcttccaa ttgagccaaa attttgccga
      961 ttaaattacg gcgtgtagca tccggcttaa taatagaaag cgtttgttga atcatcatct
     1021 tctcctattt aacttgcatt cccggctgaa caccgctgtc tgcatctaat aagaacaggt
     1081 tcgcaccgcc tgtgccggca gataaaatca ttccctccga cataccgaac gacattttgc
     1141 gtggagcaag gttggcaatt acaatcacaa aacgcccgat aagtgcttcc ggatttgggt
     1201 aggccgcctt aatgcctgag aatacttggc gttggtggtc gcctaaatcc agctcaaaac
     1261 gtaacatttt gtctgatttt ggcacagcct cgcagttcaa tactttggct gcacgtaaat
     1321 ctaatttcgc gaaatcatca atcgtaattt cagggctgat tggctcaact gcaatatttt
     1381 cttgtttttc gaccgcttgt tgttccactt tttgtgttcc tttggtttgg ttttcggttt
     1441 tcttattcgc ttcggcaaat aaggctttgg tttcatcaat cactgcatca atctgttttt
     1501 tctcaaggcg tgagaatagc gatttaaacg gtgccacggt atgcccgaat aatggggtag
     1561 cgatattatc ccaacgcaat tcgctttgta agaatgcttc ggcacgttct gccagttttg
     1621 gtaacaccgg ttttaggtag ctcatcagca cacggaataa ctcaatgccc attgaacaaa
     1681 ccgcttgtaa ttcagcctct ttgccttctt ctttggcgat gacccaaggg gctttgtcgt
     1741 caatgtattt attggcttta tcggttaatt ccataatggc acgaatagct ttattaaatt
     1801 cacggttttc atagaaattc gctatggttt ctgcttgagc ggtaaattca ttaaataacg
     1861 cttcgtcatc taatttttca gctaatttgc cctcaaagcg tttagcgata aaactggcgt
     1921 tgcgagaagc aagattcact aatttgttta cgatatcgct gtttacacgt tgcacgaagt
     1981 cgtctaaatt aaaatctaaa tcttcaatgc gatcgtttaa ttttgccgca tagtaatagc
     2041 gtaagcattc cggatcgaga tgttttaaat aggtgttggc ttgaatgaat gtaccacgag
     2101 atttcgacat tttcgccccg tccactgtca cataaccgtg agcaaacaca ttggtcggtt
     2161 tgcggtagcc gctgccttcc aacattgccg gccaaaacag gctgtggaag taaacaatgt
     2221 ctttaccgat gaagtgataa agctcggtgg tggaatcttt ttgccaaaac tcatcaaaat
     2281 caatgcctgc acggtcgcat aaatttttaa acgatgctat ataaccaata ggggcatcta
     2341 accaaacgta gaagaatttg ttttctgcgt ccggaatttc aaaaccgaaa taaggggcat
     2401 cacggctgat gtcccattgc tgtaaaccac tttcaaacca ttcttgcatt ttattggcaa
     2461 tttcaggttg gagtgagccg gaacgagtcc actctttcaa cattccctca aagcttggca
     2521 ggtcaaaaaa gaaatgttct gattctttta ccaccggagt tgcaccggaa actgccgagc
     2581 gagggttgat taaatccatc gggctatagg tagaggcaca tacttcacag ttatcgccat
     2641 attgatcttc ggctttacat ttcgggcagg tccctttcac aaagcgatcc ggcaagaaca
     2701 tatttttttc tgggtcgaac agttgtgaaa tcactttgct tttaataaaa ccgttcgctt
     2761 ttaaggtttt ataaatatcg gtagtgattt ggcggttctc ttcgctgtgg gtggaatggt
     2821 aattatcaaa gctgatatta aaactggcaa aatccgcttc gtgatcggct ttggcttttt
     2881 cgattaactg ctcaggggta atgcctaatt tatcggcatt aagcataata ggcgtgccgt
     2941 gggcatcatc agcacagaca aagtggactt cattgccacg catacgttga aaacgcaccc
     3001 aaatatctgc ttgaatatgt tcaagcatat gacctaaatg aattgcaccg ttagcatagg
     3061 gcaaggcaca agtaacaagc atttttcgtt tttggttagc cattcttttt ctcattaaaa
     3121 ttagggtaaa aattgccgtc tattttagcc caaaagtggc aggtttacca gttggcgtga
     3181 atttattaga gctttgggct aacaagcggt agaatatgat gcgctagagg cgaaacattt
     3241 ttcgcctcaa tttcccaaat gatgatgtgg gcgaaagatg tttcgcctct aaacctattt
     3301 tggataaaaa tagtatgcga caagtaaagt tataccgcta ccaactcccc attcaaaccg
     3361 gtgtagtgtt acgcaagcaa cctttgaaag aaaggcaagg ggtgttggtg tgtttgcaag
     3421 aacaggagaa agtcggctgg ggcgaaattg caccgcttcc aagttttagc caagagagcc
     3481 tagaacaagc tgaaaaccaa gctgtgaatt ggttaaaggc gtggcagaaa ggagagcctg
     3541 aaacgttaga gcagcttttc ccgtctgttg cctttgggct aagctgtgcg ttggctgaac
     3601 tgaataatga attaggcgag caagcgaata accaatcggc ggtgctgtgc tatggcgatt
     3661 tggaaaagtt acacacggca tttagccaac atacgaaccc aatcggtaaa attaaaatcg
     3721 gtctcaatcc tgaacaggaa ggcgagcagg caaatttgct gttgcatact tttccgaatt
     3781 tacgcctaag gcttgatgcc aaccgtcttt ggagcttgga acaagcggtc aaatttgctg
     3841 aaaaaattgc aaatgaacat aaaccccgta ttcaatttat cgaagaacct tgccaagcgc
     3901 tcgaactttc ccgccaattt gccacacaaa ccgacattgc gattgcgtgg gacgaaaccg
     3961 tgagagaggc gaattttttg gtaaaaaaag agccaaattt gaccgctatt atcattaaac
     4021 ctacgctggt tggttcgctt gaaaaatgcg tgaatttaat tcaacaagca cagaagcaag
     4081 gattagtagc ggtgattagt tcaagtcttg agagcagttt gggcttaacc caactggctc
     4141 gtatcgccca acaatacacc ccaaacagtg tgccggggtt ggatacgtta aatttaatgc
     4201 aacaccaatt attgcgagat tggcacgact ctagcttgcc gttggtcggg ctagaaagcg
     4261 aatttgttag ggaagttgtg gtataatctc ccaattaaac actctctccc cttgtgggag
     4321 agagaccgct tatgagcgtt cagcgaaata agcagagaga ggggaagcgg tcatttttct
     4381 aaaaaagttt gcacatttcc cctctccctg atttttcttc tggacgaaga cgtatttccc
     4441 ccctctccct gatttttctt ctagacgaag aaaaatctgt ccctctccca cgaggggaga
     4501 gggtaaagta atttatggta taaaaatagt atgcgtttag acaaatttat cgccgaaaat
     4561 accggtttga cccgttcaca agcggctaaa gtgctgaaaa gtggtgtggt gacggtaaac
     4621 ggcaatattg aaaaaagtgg tgcggcaaaa atcagtcaag aagatgaaat ttattacgaa
     4681 gatcagaaat tagagtgggt ggaagagggg cagtatttta tgctctataa gccacagggc
     4741 tatatttgtt cacacgatga tggtgagtac ccaacggtgt ttcaattctt tgattatccg
     4801 ttaatgacta aattgcatac cgcagggcgg ttagatgtgg ataccacagg gctggtgctg
     4861 ttaaccgatg acggcaaatg gtcgcaccgt attacctcgc ccaagcacca ttgtgaaaag
     4921 acatacttag tgacgcttgc tgatccggtg gaagattttt atgcacagcg attggcagaa
     4981 ggaattttat tgcgtggcga gaaagagcca accttgccgg ctcaacttga aattttagac
     5041 gattacaatg tgaatttaac tattagtgag gggcgttatc atcaagttaa gcgaatgttt
     5101 gccgcccttg ggaataaagt cgaggcattg caccgctggc gaattggcga tgtggtgtta
     5161 gatgaaaggt tggaagaagg tgaattccgc tcgctcactt cagaggagat agccagtttt
     5221 aatggaaagt agaagaccaa gcctgagctt tttcatcata ttaggtatga tggcaatgtt
     5281 gccaccatta gccattgata tgtatttgcc ctcattttta gacattgccc gtgacttggc
     5341 ggtttcgcaa gaaaaagtgc aaaccacctt ggcggtattt accctaggtt ttggggcagg
     5401 gcaactattc tggggaccga tggcagacag ctttgggcgg aaaatcatta ttttaatcgg
     5461 attagtcggc tcggcgattg ccgcttattt tctgacccaa gttcagaata ttgaaacatt
     5521 ttacctgcta aggttagtac agggcttatg tgcggcagcc ccggcggtgg tgctgggagc
     5581 attagtgaga gatctgtttg agcgtaattt atttgccaaa ttgatgtcgg ttattatgat
     5641 tatttctatg cttgccccat tgcttgcacc gattgttggc ggttatattg ccaaatattt
     5701 ccactggcac tcaattttct atgtgttagt ggcgatgggg attttgtgcg tggtattaat
     5761 tagttggaaa atccctgaaa cattagcccc cgaaaaacgc ctgccgttaa gttttggcaa
     5821 aactttaagc aattttgcca tcttactgaa acataggcct actcttggtt atgtgctgat
     5881 tggcggctta acctttgcag gtattttctg ttttttaacc tcaggctcga ttgtctatat
     5941 tgggctttat ggcgtgtcgc aagaaaattt cggctatttc ttcgggctga atatgattgt
     6001 gatggtgacg atgacggcga ttaacagcaa aatggtagtg aaagtcggct ctgaaacgat
     6061 gttacgaatt gctttagcta tgcaactgtt agccggcatt tggctggctt tagtggcgat
     6121 ttttgattta ggcttctggg caatggcaat cggtattccg ctttatatcg gaatgctttc
     6181 caccattggt agtaatgcca gtgccgccat tttagagcaa tttccacaaa tggcaggcac
     6241 ggctaacgga gtagcaggta cggctcgttt tggtgttgct tcccttgttg gagcagggct
     6301 ttcacatatt gctgtaacca gtgctgcccc aatgcttttg gcgatggcat tgtgcgttat
     6361 gttagccggt gtggtgtatt attttctgtg ttataagcga taactaggtt ggggttagct
     6421 tgcgaaaacc caacaaattt ctacaagcgg ttaataatag tacatttttt gcaaattttt
     6481 aggcagaatt aaccgcttgt gtggtacgag cgtggacgct cgcaccatcg gggcaatatc
     6541 tactaagatt ttaaaaacag cgaaatagat tccaaatgcc cggtgtgggg gaacatatca
     6601 atcattgccg ctttttctag cctgtagcct agttgcaaca gtttttcggc atctcgcacc
     6661 agtgttgccg gattgcaaga gacataaaca atccgctcag gattgagtga tgtcaagtga
     6721 tctaggcaaa atagtgctcc gttgcgtgcc gggtcgagta gtaccttgtt aaacggctct
     6781 tttgcccaag gtttgtcaga aaacggctca tctaaattgg tttggtaaaa ttccacattt
     6841 tttagaccgc ttgtcaccgc attctctcgg gcttgttgca ccattggttc taccccttcc
     6901 acaccgacca ccgatttcgc tcgctcagca ataggcaagg taaaattccc cattccacag
     6961 aagagatcta acactcgatc ttggttggat aattccagcc actcaagggc tttttccacc
     7021 attttttcat tgagcgtggc attcacctga ataaaatccc gaattgaaaa atgcaacttt
     7081 agaccctgaa tttgataata cgggcgttca ccgcacagat gctcaatgtg attttcgcta
     7141 gtcatcacaa aaagagataa ttgctcttgt gtggcgaatt gacgcaaatt gtggttatct
     7201 tgcgccgata atgcccctaa atggcggaaa aatagggcaa tggtattgtc cgcactgacc
     7261 agctcaatat gccccaattt tttcggctgt tgccagtttt caatcaggtg ttttaatttt
     7321 gggagcaaat tggcaagcgg ttcaaccagc acttcacagt gtgcaagcgg tataatttgg
     7381 ttggaatttt gcaaacgaaa gccgattacc gccttgcctt gctggatcgc aatgcttaat
     7441 tttgcccgac ggcggtagtg tttatcgttg ccgataatca tcggctgaac atcaatagcg
     7501 tgggtttgca atttctgtaa gcgttggaat aaagcctgtt gttttgcctt acgttgcagt
     7561 tcaagcgaaa tatgctgcat ttggcagcca ccgcattgcc cgtaaagttc gcagcttggc
     7621 attcgccgat ttgcagattt tttaagaatt ttaaccgctt gtccacgtcc gtattggcgt
     7681 ttctcttcca acactttaaa ttccacctgc tcatcaggca gggcattttc gataaaccaa
     7741 gttttgccgt tgattttggc aatccctaag ccttgataat ctaacgattg aatagttgcc
     7801 ttttgacaag cgggtgattt tatcggtttt tttgccgatt tttcagaata aaaaatagcc
     7861 attgttttca gatgaattgt tatgatataa agtgtcgaaa tggggagatt atataagaaa
     7921 agcagttggc ggaaaatgga ggcaatatga tttcatcggt ttggaatgaa cgttatttgg
     7981 cggataaaaa acgggaacaa gatcaccgct cgccttttca gcgggatcgg gcaaggttat
     8041 tgcattcgga ggcttttcgc tgtttgcagg cgaaaacgca aattcacgcc gtgggggaag
     8101 atgattttta tcgaacccgt ttaacccatt cgttggaagt ggctcaaatc ggtagcagtt
     8161 tgcgagaaaa attattagcg gatgtggaag gctttcaagc ggtctgttcg gagcaggaat
     8221 ttgcaaaaaa tatggcaaat ctgaccgctt tattgccgga tcatagcttg gtggaatccc
     8281 tttgttttgc ccacgatctc gggcaccctc cgttcgggca cggtggtgaa atggcgttaa
     8341 attataaaat gtacgccttt ggcggcttcg agggtaatgc tcaatccttt cggattatga
     8401 cccagcttga gccttacacc gaaacagccg gaatgaatct gacccgtcgc accttactgg
     8461 gggtaatgaa atatccggca ctcttgtctc aaacctcgcc gattgagccg gcagaaatga
     8521 gcgaggctcg gcatattaat ttgcaccatt ttccacagag caaagggatt tttgatgaca
     8581 acaaccactt ttttgagtgg gtattagccc cgttaagtga aaaagaccga cgccaatttt
     8641 gcacggttca gcccaatcaa gatccgaaac agccaaacaa aacgcaatat aaatctctcg
     8701 attgcagcat aatggaacta gcggacgata ttgcctatgg cgtacacgat ttggaagatg
     8761 cgattgtagg tggaatggtc acaccgcaat cgtggcaaaa tgcggaaaaa ttgctggcgg
     8821 agtgccaatc cgattgggtg aaacaacgcc tgccggagat tcgggaaaag ctcttttctc
     8881 agcaccgcta cgagcgaaaa gatgtgatcg gggcattagt gaatcatttt attaccaatg
     8941 tgcgttggaa agctctgcct gaatttgacg agccgttgct acgttataat gcctatttac
     9001 cggaaagtgt tgcctgtgtg ctgaaaatct tgaaagattt tgtgtatcaa tatgtgattt
     9061 gtgatgccaa aacccagcgg gtggaacgca aggggcaacg cattctaatg gaattgttcg
     9121 atattttaag cagtgatccg atgcgattac tccctaataa tgtgcgggaa cgttggaaaa
     9181 atgcggaaga atctaagcgt gcaagggtaa tttgcgacta tttagccggt atgtctgatg
     9241 ggcaggcgtt taaacttttt gaaagtttat aatgaacttt gttaattttg caaggtctca
     9301 gtaataggtt tcatttattt aataggagat ttcaatgaaa actgtttcta aaggtctttt
     9361 agtattagca actttggctt ctgcaacagc atttgcggtt tcttccaagt gaaatttatc
     9421 agcctcgtgg cgaattagtt aaagcagatc gtcaaggtaa tggtgagttt gaagtagaat
     9481 atcgtgtcag tggtaatgat gttcgtggct tagcgaaaag tgcgatcgcc cacgctaaac
     9541 gtaaaggttt ccatttagta gaatctgata ttcatagaga tgatgctgat ttaaaattca
     9601 aacgtggtga ccaagagctg gatattgaaa ttgaagtaaa aggtagaaat cggattgaat
     9661 ataaggcaga tctagattta gataaaaact aaatttaccc cttaaaacga ccgcttgtgt
     9721 gatttaaccg cacaagcggt ctttttattg tgcttttttg caaaaatact caagccgacc
     9781 ggtcccggaa tttggtaaaa tagccgttta aatgacaata atgatgcttt tacttattac
     9841 ttagaggaaa aaatgttttt agtcggtcag cgttggctaa gtgaatcgga aaataattta
     9901 ggtttgggaa tcgtgacttc ggtggatcat cgcactgtca ctatcaattt cccggcagcg
     9961 gaagaagagc gggtctatgc aattgcagtt gcacctttaa ctcgagtgca gtttcaagcc
    10021 ggagatagaa ttaacagtat agatggctgg cagttagagg tttcagaggt tgtggaaaat
    10081 caaggtgccg taatctattt gggcaaaagg gtggataacg gtgaagaagc ggtactgcct
    10141 gaaatgttgc tcgatcataa aatcagtttc agcaaaccgc aagaccgcct tttttcggct
    10201 cagattgatc gctcagatcg ctttgcatta cgctttaacg ctttgcagca ccagcaagcg
    10261 caatatcagt cgccactgcg tggtttgcga ggcattcgag ccagcctgat tccacatcaa
    10321 ttacatattg caaaagaagt cgggcaacgc cttgctcctc gtgtgctatt agccgatgaa
    10381 gtgggactgg gtaaaactat tgaagctggt atgattttgc aacaacagct attttcgggc
    10441 agagcagaac gagtattggt attagtgccg gaaagtttgc aacaccaatg gttggtggaa
    10501 atgcttcgcc gttttaatct gaaattctcg ttattcgatg aagaacgttg tagtgatttt
    10561 gataaacaag atgaaaatgg cgatgatgta agtgaaaatc catttgagag cgaagcgtta
    10621 gtcattactt cactaaactg gctggaaacc tcaccgcaac gagccaatca agtgcttgct
    10681 gccgactggg atctattaat tgttgatgaa gctcaccatt tagaatggtc ggaaaccgaa
    10741 tcttctatcg gctatcagtt ggtagagcgt ttatctcaac aaattcctgc cgtcttgctt
    10801 ttaacggcaa caccggagca attagggcaa gagagccatt ttgcccgctt ggcgttgtta
    10861 gattcggatc gtttttatga ttatgccgag tttgtacagg agcaagcaca gtataaaccg
    10921 gtggcagatg cggtgagtag cttattaagc gataaaccgc taagtaatga cgagcagaat
    10981 gcgattgcgg agctgttaag cgagcaagat accgagccga tgttccgtgc aattaatagc
    11041 aaaaaatcgg acgaaaatga ccgcttgcaa gcccgtcaag agctgattag tgagctaatt
    11101 gaccgccatg gaaccagccg ggtgttgttt agaaataccc gtcagggagt gaaaggcttc
    11161 ccacatcggg aataccacca aatcagccta gaaatgccga aacaatacca aaatgcgtta
    11221 aaagtgatgg gaatgatggg gagtgtgagc caaagtgatt tgctctatcc tgaatatttg
    11281 ttccaacgaa tgaacccgaa tgcgaaatgg gctgaattcg accctcggat tgattggctc
    11341 atcactttct taaaaaatca tcgtgatgaa aaagtgctgg tgatctgcaa acaggcggac
    11401 actgcgattg ctttggagca agttttgcgt gagcgtgagg cgattcgttc tgccgttttc
    11461 cacgagaaaa tgtcgattgt tgaacgtgac aaagctgccg cctattttgc tcaacaagaa
    11521 gaaggcgctc aggtgctgat tagttcaagc atcggttcag aagggcgaaa tttccaattt
    11581 gctaaagatt tagtgctgtt taatttgccg gataacccgg atttattgga gcaaagtatt
    11641 gggcgattag atcgtatcgg acagaaaaat gacattcaga tttatgtgcc ttgctttaat
    11701 ttatcggctc aacagatgtt ggcagagtgg tatcacaagg gcttgaatgc gtttgaagaa
    11761 acctgcccga tgggaacagc gatcttccgt gaattaggtg aagaattgca acaattcctg
    11821 caaaaacctg aactttcagc ccaatttgat gattttgttg cgaaaaccta taaacgccaa
    11881 cagtatttga aagccgaact ggagaaagga caggacagat tattagaact gaattctaat
    11941 ggcggagaag cagctcaaca gctcgctaat gatattgcga aagaagataa taatccgcag
    12001 ttggttaatt ttgccttgag tttgtttgat gtgattggcg ttgaacagga agatttaggc
    12061 gagcagagca ttgtgattag cccaaccgga catatgcttg tgcctgattt cccggggcta
    12121 tcggaagatg gcacgaccat tacctttaat cgtgaactgg cgttaatgcg ggaagaggtg
    12181 gaatttctca cttgggatca cccgatgatc cgcaatggta ttgatttaat cacctccggc
    12241 gatattggca aatcagcaat ctcattgctg gtgaataata aattgcctgc cggtacatta
    12301 ttacttgagg cgctttatgt ggtggaagca caagcaccga aagggctaaa tttaacccgt
    12361 ttcctaccgc caaccccgat tcgggtttta gtggataaca aaggcaacaa tattgctccg
    12421 caggtctctt ttgccgggct tgaacgccaa ttaaaaccgg tgaataaaca gatggcaaat
    12481 aaaattgcca aaatggctcg agcggatatt gaaaaattaa ttaaagcgag tgaaaaagcg
    12541 gtagaacctc aggtccaaca ggtgattgaa actgccaaat ttgaggcaaa tagcaaatta
    12601 aaagcagaat tacaccgctt ggaatcactc aaaatggtga ataaaaatat tcgagccgat
    12661 gaagttgaag ccttagaaca acagcttaac gcttcccttg cccaacttga aacggcaaat
    12721 tatcgcttag atagcttgcg agtgattgta agtaataaag cgtaattttt tgcaagtacg
    12781 aaaagtgatt ttttcgatct gtgtcgaaaa aatcactttt ctattttgtg tcatccttgc
    12841 agattattta tgctattcct catattgtaa aaggtataaa aaaggaatat tatgtatata
    12901 gatttgcaat atgctgtgcc gttattgttt gaatacgacc cgaacaaaag ccaaattaat
    12961 ttagccaaat atggtattga ttttgaacag gcaaagttgc tttgggaaga tgaacgaaaa
    13021 gttgtactgg aggcttgcat tgaacccgaa ttacgctatt tcctgattgg caagatttat
    13081 gataaatatt ggacagcgtt ttttacacca agaaatgatg ttattcgttt aatttcagtt
    13141 agacgttccc gtaaaaaaga gatctcatac tatgagaaat accattagtg ccgaagaatt
    13201 agaacgtatc ttcgatgaag gcaaagaaga tattttacaa tactgcgatc tttctaccgc
    13261 tcatcgcccg gggcaaatga aaagggtgaa tgttgatttc ccaaattgga tggtagaagc
    13321 gttagataag caagccagac atttaggtgt gcctcgccaa gccttgatta aaatgtggat
    13381 tgcggaaaaa ttaccggttc aatcacctag gtaagcgagt ggcaaatggc attaattgaa
    13441 tataaccctc cattagaacc atttttagat gaggtttatc gggataacca tattttagta
    13501 attaataagc ctagtggttt attatcggtg ccgggcaatc gtcccgaata ttacgacagt
    13561 gcaatgactc gagtacagca aaaatatggc tttaccgaac cggctcatcg tttggatatg
    13621 gcaacaagcg gtattctttt gttcgcccta agcaaagcgg cggaaaaaga actaaaacgc
    13681 caatttcggg aacgtgaacc gaaaaaacac tatattgcgt tactttgggg caaattaggt
    13741 gaaaaagttg gggataaggg cgaaatgaat tttccgctga tttgcgattg ggaaaatcgc
    13801 ccccgccaga aaatttgcta tgaacgaggc aaaaaagcca ttacccatta tgaagtgtta
    13861 gctcattatc cgaataatat agcgagagta aaactcacgc ctatcacggg gcgttcgcat
    13921 cagctacgag tacatagcct tgcgttaggt cacccgatta ttggcgataa gttttatgct
    13981 aatccgctgg caaaaagttt atcgccccgt ttatgtttgc acgcggagtc actaacaatt
    14041 actcacccga ttacaaggga gttgatgacg tttactgctg agccgacttt ttagcctcgt
    14101 tcagtaggtg tttgtaatat gcccaatcaa aatattgccc cgggtcgatt ttccgttccg
    14161 gggcaatatg gcaatgcccg gtgatgcgtt catcggtaat ttgtggatag ctttgctgaa
    14221 tggctttggt caaatttgcc aaagattggt attgagcttg agtaaacggc tgattattac
    14281 tgccttccag ttcaatgcca atcgaatatt cattgcattt ctcccgccct tcaaaacaag
    14341 agacgccggc gtgccacgcc atatcgttaa aattcacata ttgcgtgatt tccccctccc
    14401 tattaatcaa acaatgggcg gaaacccgta gatctttgat ttcttcaaaa taagggtgag
    14461 cggttggttc gagtttacct tggaaaaaca gatcgataaa atttccacca aattcttccg
    14521 gcggtaagct gatgtagtga ataaccaaca aggaaatatc ctcttgatca ggacgttggc
    14581 taaaatgcgg agacggcact tgtttttgac cggttaaaca gccctgttcg atttttaaat
    14641 ttggcatagt ctaaataggg tgaatacgtt aaaatatggc tcattttact agaaaaagag
    14701 aagaatatgt taagttatcg ccacagtttc cacgcaggca accacgctga tgtgctaaaa
    14761 cacattgtcc agcttttgat cttgcgagca ttaaaagaaa aagaaaaagg ttttttatat
    14821 cttgatactc actccggtgt ggggcgttat agtctgttaa gttttgaatc ggaaaaaaca
    14881 ggcgaatatt tagagggcgt tgcccgttta tgggagcgtg atgatttacc ggaagaagtg
    14941 gcattctata ttgatgagct gaaaaaatta aaccgtggcg gcaagttaaa gtattacgca
    15001 ggctccccat tattggcggt acagcaactg cgtgagcaag accgagccat tcttaccgaa
    15061 cttcacccca atgattttcc gctgttacga caagaatttg ccaaagcgaa aaatgtgcaa
    15121 accaagcgag aaaatggctt tcaacaatta aaatccgccc tgccgcctaa agagcgtcga
    15181 ggttttgtgt tgattgatcc accttatgaa ttaaaagaag attatgaatt ggtggttaaa
    15241 gcgatagaag agggctacaa acggtttgcc actggtgtgt atgccatttg gtatccggtg
    15301 gtgcttcgcc aacataccaa acgtatagtg cgaggtttaa tggataccgg cattcgcaaa
    15361 attctacaaa ttgagcttgc cgttcgtcct gatagcgacc aacgtggaat gacggcaagt
    15421 ggtatgattg tgatcaaccc accgtggcag cttgaaaccc agatgaaaaa aatcctgcca
    15481 tatttaaccg aggtgcttgt gccggaagga acaggttctt ggaaggtgga gtggattacg
    15541 cctgagtaaa ttaaatacgg atagcacaag cgtggacgct tgcgccatca taaattctga
    15601 tttgcaatat tttttagaaa aataaccgct tgtcttatca agaagtgggg gaaatataaa
    15661 aggaaaaaac gatgcaacaa tacaaacacg actttattga atttgcctta agccgcaatg
    15721 tgctgaaatt tggcgaattt accttaaaat caggcagaat cagcccctat tttttcaatg
    15781 ctgggctttt taacaccggg cgcgatttag ccaaattagg cgaatattac gccaaagcca
    15841 ttcaagccag cggcattgag tatgatgtgt tattcgggcc ggcttataaa ggcattccga
    15901 ttgcaaccac ggttgccgtg gcactagcca acctgttcga tgtggataaa ccttgttgct
    15961 ttaaccgcaa agaggcaaaa gatcacggtg aaggcggtaa tttaatcggt tctcccttaa
    16021 cgggcaagat tttgctggtg gacgatgtga ttaccgcagg cacggcaatt cgtgagtcga
    16081 tggaaattat caatgccaat aatgcccagc ttgccggtgt gctgattgcg ttaaaccgta
    16141 aagagaaagg caagggcgag ttgtctgcga ttcaagaagt cgaacgtgat tacggttgcc
    16201 aagttttctc gattgtggat tttgatgatt tggtagcatt tattgaaacg tcgccacaat
    16261 acgctcctta tttgccgaaa atgcgtgaat atcgtgaaaa atacggtgtt taacttttgc
    16321 aaaaaaacag caaaaactca ccgcttgtca ctctctaatc tcaccattat gaatcacttt
    16381 catcttttgg tgagattttt tcaattaaaa agccgataaa ttgattgtga gctttagcta
    16441 attggcttag gataaactcc tgtaatacag gagttggaga actaaaatgg cacatcaaaa
    16501 attacctttt cacgcagaca ccgttggctc atatttgcgt tccgatgctt ggaaaaaagc
    16561 tcacgcagac tataaagcag gcaacatttc cctcgaacaa cgtgatgaaa ttgtagaagc
    16621 ggaagtgaaa aaattagtac aagcatcaat tagatgcggg cattcaagtt gttacagacg
    16681 gcgaatatca ccgctcttgg tggcatattg atttcttgga aaatctgaac ggcattgaag
    16741 gctatgtgcc ggaaaaagcc tatgcgttta aaggggttga ggttcgccct tataatacac
    16801 gttgttgcgg taaagtgtct tggaatccga atcacccttt tattgagcat ttcaaaaaat
    16861 tcaatgcgat tgtaggtggt cgtgcggtgg cgaaatttac cattccaagt ccaaaccaat
    16921 taatgtaccc gattcagtgg gatcacggca tttacccaaa ccgtgaggaa tttgccaaag
    16981 atgtgcaaca ggcttataaa gaggcgatta aagcgttcta tgatgccggc tgtcgttatt
    17041 tgcaaattga tgatgtctat tgggggtcgt tgtgtaataa cgttggtcaa ccgacatttg
    17101 aagccgataa agctcaagcg gtggcgaata ttcaagccat tcttgccgat aagccggaag
    17161 atttaaccat taccacccac gtttgccgag gcaactataa atcttcttat ttattgacag
    17221 gggcttatga tccgattgcc gacggcttat ttgctcaaac caactatgat gggtatttct
    17281 tagaatatga tgacgagcgt tcaggcggtt ttgagccgtt aaaacatttt gccaacaaca
    17341 aagggcgtgt ggtattgggc ttaattagtt caaaattccc tgaattggaa gataaggaag
    17401 cggttaaagc ccgtattcaa gaagcggcaa aatatgtgcc gttagagcag ctttgcctaa
    17461 gcccgcaatg tggttttgcc tcaaccgaag agggcaatat tatgtcggaa gcacagcaat
    17521 gggcgaaagt tcgtcttgtg gaggaaatcg cccaagaagt ttggggcgaa gattaattag
    17581 agtgaaaaat gcccgtaaca agcgggcatt ttttgtgaat tttttgcaaa ttcaagccat
    17641 ttagcgaatg cggttaaaac cttgttctaa atcggcaatc aaatcttcca ccgcttccaa
    17701 gccaacgtga atgcgaatta atgttcccgt cagtttgcgt tcaatcgccg ggcggatttt
    17761 ggcaatttct tccggctgat tggcaagaat cagcgattca aacccacccc aagaatacgc
    17821 cattgaaaac agttcaaaat gctctaggaa gttggctaat ttgtcattcg agagcttttc
    17881 gcaaagttca aatgaaaata gaccgcttga acctaaaaaa tcccgcttaa aaaattcgtg
    17941 ccccgggcag ctttccaacg ccgggtgata aactgctttc acttcagggc gttgagccag
    18001 ccagttagct accgcaatgc tactttgttc gtgctgtttt aagcgaatgc ctaaggtgcg
    18061 tagcccccga gcggtgatgt aagcggaatc agcatccacc atttgcccca ttaaataaga
    18121 gccttcacgc agtttatccc aacaacgggc gttagaaacc gcagtaccga tcatcacatc
    18181 agaatgcccg actaaatatt tagtgccggc ttgaatggaa atatcaatgc catattctaa
    18241 agcagggaac agcacaccgg cagaccaagt gttgtcgatc ataatcacga tttctgggtt
    18301 gatttcacga gcaacacgca cgagagtcgg aatatcaggc acttccattg ttagcgagct
    18361 cggcgattct aaaaaaagca ctttggtgtt cggttgcaca agggctttca agccttcacc
    18421 aatcatcggg tcataataag tagttgaaac acccatacgt tttaacagcg cattacagaa
    18481 ttcttgtgtc ggttcgtaag ctgcgcctgt cattaaaacg tggttgcctt gttccacaaa
    18541 agctaaaatc gcattggtga ctgccgccgc accgcaaggg taaaggtagc aacctgctcc
    18601 accttctaat tcacacatcg cttcttgtaa tgaaaaatga gtgagagtgc cacgtctgcc
    18661 ataaaataat gctcctttgt agcggttttg tgtagcgtgt tttttctctt ctaaggtttc
    18721 aaacactaaa gatgaagcac gctgaatcac cgggttaact gctccttgtg tgttacgttt
    18781 tttacgccct gtatgaacaa gtttggtgga aagattttta tagttagaca taacttttcc
    18841 taaagtaaaa tgcatagggg caaatcaaca tttgcccctt tattaataat taacgaaaca
    18901 taaagttatt tctttgacgg ctacgtttaa attggctgat tagcacaatg accgcaacaa
    18961 ttaagtaaac cgcaaaagta aagattagcc attgtgccat tgaaaagccg aacatttccc
    19021 attggctttg gctacaatta accggaccgg gggcaaacac ttgtggtagc cattggtcaa
    19081 gcggcaaggt ttgcgggaaa ttaactttaa attcgcattg attccatggt gatggattga
    19141 cctgataatc gtggtgtttg attgaaagta ctagcccttt aaatgcgcta ccaagccaaa
    19201 ttagaattgc caagtagcga ataatcgcat atctattacc aagaaatcct aaaataccgg
    19261 caaataaaat accaaataaa gcaactcgct cataaataca cattacacaa ggtactaatc
    19321 ccatcccatg ttggaaataa agcgctgtgc cttcaagtat aagacagctt aacattagta
    19381 aaaaccatgc actgcgagat tgtgatagat ttttaaaaaa atagagcata gttattcctc
    19441 attttgtgaa cttaaggcat tctaccttat ttggtatgag ttatctataa actaacgtgt
    19501 aataaaccat ccccaattta tcattacttc agtcatagaa ggtagtaaga attctaaccc
    19561 taagaaaccg acaatagata gaacaatggt ataaggtaat gccatataga ccattctgcc
    19621 gtaagacagt ttaattaacg gcgcaaagga ggacgttaat aagaataaga aagcagcttg
    19681 tccgtttggg gtagcaacag aaggaagatt tgtgccagta ttaattgcaa ctgcaatcag
    19741 gtcgaactgc tcacgatcta taatgccggc atctagtgcg gttttagctt cattaatgta
    19801 aacagtaccg acgaagacgt tatctgaaat catcgagagc aaaccattga aaatatagaa
    19861 taatgaaagc tgtgaatgtg gttcggcaga taacacaaag gcaatgatcg gctcaaataa
    19921 ttttaaatca ataatcacgg caacaaccga gaaaaacaca ataagcaatg cagtaaaagg
    19981 catagattct tggaatgctt tccctaaagt gtgttcatca gtaatgccac aaaatgcagt
    20041 gcaaataatg atgatggtta agccaataat ccctacatcg gcaagatgta gcgctaagcc
    20101 aataattaac caaattccag caatggcttg caccagcatc tttaagcgat cttgtttcgt
    20161 cattttttgt tcttttagtt ggctatagcg agcgagtacg ccccaaactt tgctaggaag
    20221 ccggtctcca taaccaaata atttatattt ttctacaaga aagcaggtcg caatcccaca
    20281 aattaacacg ggtaagctaa ctggtaatgc tctaagtaag aattcgataa atccccattc
    20341 cgcttgggag gcaataatta agttttgtgg ttctcccacc atggtcatta cgccacctaa
    20401 agctgagcca ataccggcgt gcatcaataa acttcttaaa aaaccacgaa attgctctaa
    20461 aatttcttta ttagagatga ttttttcatc attggtaata tcagtagaat cttcaaagtt
    20521 attacctgat gcgactttat gatatacgcc gtagaaacct gttccaacgc taatgattac
    20581 agcaataacc gttaaagcat caagaaaggc ggataaaaaa gcagcgctta agcagaaaga
    20641 aagggaaaga atttttttcg aatgaatggc gacaagcagt ttggtgaaaa tataaagcaa
    20701 taattgcttc ataaagtaaa taccggcaac cataaacatt aaaagaagaa taacatcaaa
    20761 gtttgccata atttcttctt taatgtggtg aggatttgtc ataccgatta caactgcttc
    20821 aatcgctaat aaaccacctg gctgtaatgg ataacatttt aatgccatag agagagtaaa
    20881 aataaattca gcgactagaa accagcctgc aacaaaagga ttgataaaga aataaatgag
    20941 aggattgata attaaaaatg ccaaaatgca caatttatac cactcggggc tttttcctaa
    21001 gaaacttttg aacagagcat caatgctact catataaagg acctcaaaat ttgt
//
LOCUS       DANLXQ010000024        21028 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.024, whole genome shotgun sequence.
ACCESSION   DANLXQ010000024 DANLXQ010000000
VERSION     DANLXQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21028
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.024"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            5..505
                     /locus_tag="PXD94_000706"
     CDS             5..505
                     /locus_tag="PXD94_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL6285597.1"
                     /translation="MSNIGLDKEIAKKLADELNGLLATYQVFYTNVRGYHWNVRGVNF
                     FELHAKFEEIYDDLVTKVDEIAERILTLGHTPQNAFSQYLTKSQIQEHIGVSAAQECL
                     RGTLNGFKTLLGQQREILALANDANDEGTASQMSDYIKEQEKLVWMFTAACAECSDYA
                     QGCSCN"
     gene            564..1277
                     /locus_tag="PXD94_000707"
     CDS             564..1277
                     /locus_tag="PXD94_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL6285598.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGIGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     DNCYTTEFKRLTQDFYLNF"
     gene            complement(1388..2392)
                     /locus_tag="PXD94_000708"
     CDS             complement(1388..2392)
                     /locus_tag="PXD94_000708"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL6285599.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRAKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
     gene            complement(2480..3319)
                     /locus_tag="PXD94_000709"
     CDS             complement(2480..3319)
                     /locus_tag="PXD94_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL6285600.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     LPE"
     gene            3509..4843
                     /locus_tag="PXD94_000710"
     CDS             3509..4843
                     /locus_tag="PXD94_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL6285601.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(4923..5333)
                     /locus_tag="PXD94_000711"
     CDS             complement(4923..5333)
                     /locus_tag="PXD94_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL6285602.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(5456..6133)
                     /locus_tag="PXD94_000712"
     CDS             complement(5456..6133)
                     /locus_tag="PXD94_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL6285603.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIMPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            6251..7063
                     /locus_tag="PXD94_000713"
     CDS             6251..7063
                     /locus_tag="PXD94_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL6285604.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(7111..7380)
                     /locus_tag="PXD94_000714"
     CDS             complement(7111..7380)
                     /locus_tag="PXD94_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL6285605.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(7353..8030)
                     /locus_tag="PXD94_000715"
     CDS             complement(7353..8030)
                     /locus_tag="PXD94_000715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6285606.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(8132..8389)
                     /gene="rpmA"
                     /locus_tag="PXD94_000716"
     CDS             complement(8132..8389)
                     /gene="rpmA"
                     /locus_tag="PXD94_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL6285607.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(8410..8721)
                     /gene="rplU"
                     /locus_tag="PXD94_000717"
     CDS             complement(8410..8721)
                     /gene="rplU"
                     /locus_tag="PXD94_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL6285608.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            8999..9997
                     /gene="ispB"
                     /locus_tag="PXD94_000718"
     CDS             8999..9997
                     /gene="ispB"
                     /locus_tag="PXD94_000718"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL6285609.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQCVAIAADASEEIETALKNYGCYLGTAFQLIDDI
                     LDYSAQSESLGKNVGDDFVEGKPTLPLLHAMHAADKAGKTTQAELIRNAIENGGELEQ
                     IPQVLAIMQEHQSLDYAMQKAREEAQKAVDSLGVLPESEYKQALVSLAYLSVDRSY"
     gene            10100..10846
                     /locus_tag="PXD94_000719"
     CDS             10100..10846
                     /locus_tag="PXD94_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL6285610.1"
                     /translation="MTEQNQRPQEHIESVRARRRAQNQKKGPNAPFVREKLTLPNGHN
                     KLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKWGIP
                     FIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVFTSC
                     LGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQEYCG
                     CVYSLRDSNKWRLETGRQRIEIGKLYYSPD"
     gene            11263..12198
                     /gene="fabD"
                     /locus_tag="PXD94_000720"
     CDS             11263..12198
                     /gene="fabD"
                     /locus_tag="PXD94_000720"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL6285611.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            12215..12679
                     /locus_tag="PXD94_000721"
     CDS             12215..12679
                     /locus_tag="PXD94_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285612.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            12700..13422
                     /gene="fabG"
                     /locus_tag="PXD94_000722"
     CDS             12700..13422
                     /gene="fabG"
                     /locus_tag="PXD94_000722"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL6285613.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNPGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            13541..14839
                     /gene="brnQ"
                     /locus_tag="PXD94_000723"
     CDS             13541..14839
                     /gene="brnQ"
                     /locus_tag="PXD94_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL6285614.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIVSAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISANTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGVIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            14885..15709
                     /gene="mutM"
                     /locus_tag="PXD94_000724"
     CDS             14885..15709
                     /gene="mutM"
                     /locus_tag="PXD94_000724"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL6285615.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWTVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLGILQERQQKEIGKHDHVDLITQNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFAQVLQVYGRKGETCNDCGKPIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(15792..16226)
                     /gene="dtd"
                     /locus_tag="PXD94_000725"
     CDS             complement(15792..16226)
                     /gene="dtd"
                     /locus_tag="PXD94_000725"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL6285616.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(16201..17046)
                     /locus_tag="PXD94_000726"
     CDS             complement(16201..17046)
                     /locus_tag="PXD94_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL6285617.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GNGALSLGQHLLELMPFLLIWLLFTLVYKFIPIRKVKFQHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSINDR
                     LNSAG"
     gene            complement(17048..18286)
                     /gene="proA"
                     /locus_tag="PXD94_000727"
     CDS             complement(17048..18286)
                     /gene="proA"
                     /locus_tag="PXD94_000727"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL6285618.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGKETKFTNAVLVDVV
                     QDALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGLHQFCKENSTIPV
                     IVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEKAIAVEFLPKLVA
                     KMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQQAVEHIRHYGSQ
                     HSEGILTSDYQLARQFVQQVDAAAVYINASTRFTDGGEFGLGAEVAVSTQKLHARGPM
                     GLEALTTYKWVCEGDYLVRG"
     gene            18570..20453
                     /gene="mutL"
                     /locus_tag="PXD94_000728"
     CDS             18570..20453
                     /gene="mutL"
                     /locus_tag="PXD94_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL6285619.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDRQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYVQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTKLGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            complement(20472..20741)
                     /locus_tag="PXD94_000729"
     CDS             complement(20472..20741)
                     /locus_tag="PXD94_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL6285620.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(20728..20994)
                     /locus_tag="PXD94_000730"
     CDS             complement(20728..20994)
                     /locus_tag="PXD94_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL6285621.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
BASE COUNT         6238 a         4603 c         4290 g         5897 t
ORIGIN      
        1 ggcaatgtca aatatcggtt tagataaaga aatcgcaaaa aaattagcag atgagttaaa
       61 tggtttatta gctacttacc aagtgtttta cactaatgta cgtggctacc attggaatgt
      121 acgtggtgtg aacttctttg aattacatgc aaaatttgaa gaaatctacg atgatttagt
      181 gacaaaagta gatgaaattg cagaacgtat tttaacctta ggtcacacac ctcaaaatgc
      241 gttcagccaa tatttaacca agtcacaaat tcaagaacat attggtgttt cagctgcaca
      301 agaatgttta cgtggtactt taaatggttt caaaacatta ctgggacaac aacgtgaaat
      361 tcttgctcta gcaaatgatg caaacgatga aggtactgct tctcaaatga gtgattacat
      421 caaagaacaa gaaaaattag tatggatgtt cactgcagct tgtgctgaat gttcagacta
      481 cgcacaaggt tgttcttgta actaagttgc aataatataa ttaacccccg ataatgaaag
      541 attatcgggg gttacttttt cagatagcta tgtcgcaagg atttcaattt aaacagtttt
      601 ttattcggca cgacagatgt gcaatgaaag tcaatacaga tggtattcta ttgggagcga
      661 ttgctcacat aaaagattgt cagaatattt tagatctggg tataggttct ggtttagtag
      721 cacttatgct tgctcagaga acagatgatt gttgtcacat tactgcgttg gaacttgaac
      781 caaatgccta ccaacaagcg gtcgaaaatg cccaaaattc tgcatgggca aatcggattt
      841 ccgttttgca aggtgatgtg atgcagcagg catttgaaca gaaatttgac ttgattgtta
      901 gtaacccacc ttattttgca gattcacttg cagctcgaac cacagagaga gatttagctc
      961 gctcaattag ccattacagc cacttgaatt ggctggcaaa agcaaaacca tggttagcag
     1021 agaacgggaa aattacactg attttaccta tagcagctgc agaaaaatta gttgaccaaa
     1081 gtgaacaaat tggattgtat tgtgttgaac aatggttaat ttttaccaag agcggcaaat
     1141 tggctaaacg cagtatagtg agttttagtt taaacaagca aaagcgtgaa cataaagagc
     1201 tgatcatcta tgatctagat aattgttaca ctactgagtt taaaagactt acccaagatt
     1261 tttatttgaa tttttaattt actccccggg ttagtgtttt ccacaaatct tcttcagcta
     1321 cgatgatcta gccttaagct aaaaagcaga ttaccaccca agataatccg ctttttagac
     1381 ttagctatta tgccaaactt gctaccatta ccgctttaat agtgtgcatt cgattttctg
     1441 cttgctcgaa tgccacattc attggcgatt caaacacctc ttcggttact tcaatgccgt
     1501 ttgccagtgc cggatatttt tcggcgatct ctttaccgac tttagtttca ctgttgtgga
     1561 acgccggtaa gcagtgcata aatttcactt tcgggttgtc ggttgcttgc attaattcag
     1621 gcgttactcg gtaaggcatt aataaatcta tacgttcagt ccagctctct agcggttcac
     1681 ccattgaaac ccaaacatca gtatgcacaa aatcgacacc tttaattgca gtctcaatat
     1741 cttcggttac cgtcattctt gcccccgatt cagctgcaaa tttttcacac attttcacga
     1801 atgcatcttc cggtaataag gctttcgggg cacaaattcg cacgtccatc cccaatttcg
     1861 ccccaatcaa caggagtgaa ttacccatat tattgcgagc atcgccgata tagacataac
     1921 tgatttggct aagcggtttt tcgcaatttt cgatcatcgt tagcacgtca gccaacattt
     1981 gggttgggtg gaattcatcg gttaagccgt taaacaccgg cacgccggca taatcggcaa
     2041 gctcttgtac taccgattgt ttaaagccgc gatattgaat cgcatcatac attctgccta
     2101 gcactcgggc ggtatctttc attgattctt tatgcccgat ttgcgaggag ttcgggtcaa
     2161 tataggtaac gtgagcccct tgatcgtaag ctgctacttc aaaggcacaa cgagtacggg
     2221 tcgaggtttt ctcaaaaatt aaggcaatat ttttgccctt taatttttgc tgctcagtgc
     2281 cggcatattt cgcccgtttt aaatctcttg ctaaatccaa caaaaagcga atttcacgct
     2341 cagtgtggtt tactaaactg agtaaatgtc tgttttttaa gttaaatgcc attttgtttc
     2401 tccctgtaaa cattaaaatt tattttatgc aaaataattg cataaaaatt cacttatagc
     2461 aaacagaatt tgcttaccct cattcgggta aataaaactc gcctttgccg actaaaataa
     2521 cctctccgcc aacaaaaatc ttcccgtttt gatcgacttt taataacagg cgattcggcc
     2581 gttgaatttc atcaccttgt gagatctgaa gctggattgg tattttgcct tgtgcaatgt
     2641 gccaaccgcc taaatttgcc gctgccgaac cggtgccggg gtcttcgaca atcgaacctt
     2701 gcgattgaaa aaacagtcgc actttcgcct catttccctc acgacaccaa acatatacac
     2761 tgtctttcaa aaatgctttt tcagccagat ttgcatttat tttgcacttt tcgaccgctt
     2821 gttgagaagc taactcaacc aataactgct ccacgccggt gtttacataa caaggctcgg
     2881 acgcaatatc cgcaacattc agccccaata ttgaggcaca ttctgcacgg cttagcggtg
     2941 tgttttccag gttcacacca ttggttaaag caaaagtagt cacattatgt tgatgttgaa
     3001 tcgctaccaa tccggcatta gtttgcaagc gataggttgt tggaaaatca aaggcggaat
     3061 gcaacacaaa agccgctcca acagttgggt ggccggcgaa cggcatttca taatcaggcg
     3121 taaaaatttt gagttttttg accgcttgtg aatcggtgga agattgaata aataccactt
     3181 cagaaaggtt aaattggcga gcaataagtt gcatttgtaa gtcggttaat ccgtccgctt
     3241 cataaaatac tgcaagcggg ttgccgccaa aatggcttgt tgcaaacaca ttcaccagca
     3301 aataaggata gctctgcatt tatcactcct tacataaact taaagccaca ttgggcgaaa
     3361 attctaccat tttggtggcg aatcgtaaaa ttggaaaatt tatcggtatc cctctttaga
     3421 gttaactatt tttgatttat ctcaaagttt taaaaaaaag attaggtcat acttctttct
     3481 agtgattatt ttttaatttg aggttcttat gctttatgca gaatttttac ttttattagc
     3541 ctttttatat gcaggtagcc gttacggtgg catcggttta ggtgtggttt ccggcatcgg
     3601 cttatttgtt gaagtgttct tcttaggaat gccgctttcc gctccgccaa ttagcgtgat
     3661 gctcgtgatt ttggctgtag ttacttgtgc ctccattcta gaagccgctg gcggtttgaa
     3721 atttatgttg caagttgccg aacgcatttt gcgtagcaac ccaaaacgta ttacctttct
     3781 tggcccactt gtcacttatc tgatgacctt aatgctcggc acagggcatt cggtttactc
     3841 tattatgccg attatcggtg atattgcctt aaaaaataaa attcgcccgg aacgcccgat
     3901 ggcagcagcc tctgttgctt cacaattagg tattaccggt agcccacttt ctgctgcagt
     3961 tgcctattac ttaaccgaaa tcgttaaatt accgggcttt gaaggggtga ctttattgaa
     4021 tgtagtcggc gtaaccatca ccgcaacctt ctgtggtgtg attgcgatgt cattatatag
     4081 cttacgtcgt ggtaaagagt tggaagacga tcctgagtat caacgccgta tgcaagaccc
     4141 tgagctacgc aaacaaatcg agcaaacgag tgcaacgtct ttagacgaaa aactaccggc
     4201 aagtgcgaaa aactcggttt atctgttcct agccgctatt gcaaccattg tgattattgc
     4261 aatgattccg gctattaaac caacggttgc tgctacagga aaagttgccg gtatggatca
     4321 aatcattcaa atcgtgatgc taactttcgg gggtttaatc ttaattttaa ctaaaaccga
     4381 cccgaaaaaa gtaccaaacg gcatcgtttt caaatccggt atggtagcaa ccattgcgat
     4441 tttcgggatt gcttggatga gtgatactta cttcacccat gcaatgccag cgttcaaaga
     4501 aggggtaaca gaaatggttc gagcccaacc ttggacattc gccttcgcct tgtttgcggt
     4561 atctgtagta gtaaacagcc aagccgtaac cgcaaaaatg ttattaccgg taggcattgc
     4621 aatgggcttg cctgctccat tattagttgg tttaatgccg gcaacttatg cttacttctt
     4681 tattccgaat tatccgtctg atatcgcaac cgttaacttt gacgtaaccg gtacaactaa
     4741 aatcggtaaa tattacttca accacagctt tatggctccg ggtttagttg gcgtaattac
     4801 tgcctgtatt gtcggtatcg ccgttgcaaa tgtgctaatt taattttaac aaaaaaacaa
     4861 gcggtcagat taagtaaatt ttttaccaaa tctgaccgct tgattattta ctgcaaaacc
     4921 aattaggctt tgttaaagag ttttgcatct aacgagtatt ttccgccacc gataaaaata
     4981 taggttaaga ataataccga atacacgcct gctaattcac cgccgtttaa taaaggtaat
     5041 agcacattgc cttgagctcc gtgagcaata aagaacgcgt atgccatcat acctgaaagg
     5101 ataaacgctg tttggcgggt aaatagacct aaaataaaga aaattccgcc cacaatttcc
     5161 aaaataccgc ctacaccata aatggaggca aggggtacac tgccgtttcc gttggtcatc
     5221 gacattggaa attcaaaaaa tttcgctgtg ccgtgtaata aaaacatata accggcaaca
     5281 acacgggtga ttaaaaacgc taaaggttgt aaatttgtta aagtagagtt catttgcttt
     5341 ccttaaaata aattgtgata taaaggtcaa ttgattgagg caggcattct attttaaaaa
     5401 cctatacttc acaatccagc cgtcatgaaa tagattattt accattaata aacaattagg
     5461 ctctcgctaa cacccaaacc tgaatttcta ctactccagc tttgagtaac tcggcacaaa
     5521 ttacatttaa ggtggaacct gtggtgagca catcatcaac aatcgccact cgtttatagg
     5581 cttttaacgg ttgatagaaa aatgccccac gcagattttt ccgccgttca gcagcggtca
     5641 attctctttg tgagacggtt gctctcacac gttgtaaact ctgggtatcc agcggaatct
     5701 gtaaccactt cgataaccaa tcacacaata attcgctttg attaaaccct cttttccaat
     5761 gtctgtgcca aaaaagtggt acaggcataa tcacttcagg caagctcaga acctgttccc
     5821 tttgagcttg tttgattgcc agcagcaatt gtcgggcaag ggcttgatcc caccaatatt
     5881 gctgctggaa cttaaattga tgtacccaat gtgaaagtgg cgctcggtat tcatttaccc
     5941 tcactaattt atgccacttc ggttcatttt tcaaacaata gccacaagcg tttcgaaact
     6001 ccaccgcact cattccacaa tgacgacaat aaggggttgg ttttagcaat ttttggcaat
     6061 ggctgcaaat gccttgcacg ccaatagcga gtggatgatt acaatgaaag caacggaagc
     6121 cgaataaatt cacatttcct ccttttattt gcagattatc aaatttagcc ttattttttc
     6181 cggctatttt tgtgacatcg ctcgaaaaaa gagtaaaatc tcattatcag taaataaaaa
     6241 ggaaaccgag atgaaatccc tccaaaaacc gatcaaaatt gtctttttcg atattgatga
     6301 caccctctat gtaaaacata aagcctatat tccaaaatca attaccgagc aggtattacc
     6361 tcgtttaaaa gccaaaggca ttattccggc cattgccacc ggcagaaatc acggctgttt
     6421 tcctgaagct ctcaaacctt atttaaaccc tgaaatcggt tttgagctgt ttgttaccat
     6481 taacgggcaa tataatttct ataaagatca aataattagt gattattcgt taagccgtga
     6541 gcaaattgaa cgtattatcg aaaaagcgaa tgctttgggt attctgtatg cctttattac
     6601 gccaatgacg attgcagttt ccgaaaccaa tccgattgtg cacgaagcgg ttttaccgat
     6661 cacaccgaat tatttgatcg atccacacta ctacgaaaaa gagaaaattg tgcaaatgtt
     6721 actgttctac accgaagagc agaaacaagc ggtcgaaaat agtggcattt ttgcaaatga
     6781 tttaaaagag gctcgctggc accccaatgc ggtagatatt ctgcgaaaag aaaattcgaa
     6841 agctcgtggc attcaagatg tattagcaac cttgaatatt gagattgaaa gtgcgatggc
     6901 atttggcgat ggtattaacg acttggaaat gctcccgacc gtaggtgttg gtgtggcaat
     6961 gggtaatggc gtgccggaat taaaagcggt ggcagatttc atcaccaagc cgcttgagga
     7021 agacggcatt ctatacgctc ttgaaactct tgaggtgatt taaaagcaag cggttgtaag
     7081 tttttctcca tttacaaccg cttgtagttt ctaattacgc ctaaatagct tatcgcctgc
     7141 aatcgctaaa atcgccccac tgacgaccac caacgcccca agataactga tccaattcat
     7201 atccggcttg gcaaaaatat gcggataaaa atagtagcta aggttggaaa agatcatcgt
     7261 aaaaatcggc aacatcgtgg ttacaatgct aacctttgaa gtatcccaat gattcaacgc
     7321 ctcaccgtat gaaccgtagc caattaaggt gttcaacagc aataaataaa gcaaccccac
     7381 agaaaaccat cctcaagatt gcctaactgt gccggtgagg caaacggtaa catcagcaga
     7441 gaacagccga tgtaaataat aaacagcact tgctgagagt taaatttcgc taataataat
     7501 ttttgagcca cgccgtatag cacccaaatc aagctggctc cggcactcaa taacactccg
     7561 agagcgtagg tgttgagctg taaaatttca gcaaatttat cgttaaaaaa tgcgacaagc
     7621 cctgcaatca ataacacaaa tccaatcttt tgaaacaagc cgaacttttc cttaaacaat
     7681 aatacgccta gtagaatcat cgaaaatgga gctaactgcc acaatacttg gttaatggtg
     7741 ggcgaaacat agtgtagagc ctctgcaaac aaaaagaaat ttgccgacag accacacacc
     7801 cctaacaaaa ttaaaccgag ttcttttttg ctacaagcgg tcaatttggg taaacgtttt
     7861 gcaaatccta gaatcagcaa tagccctact gatgccacaa cgaacctaac ccaaactaaa
     7921 gtttgggcat ctatcacttt caatacttgc tgggcggcaa tcggtagcgt tccccaggtc
     7981 attgctgcgg ttaatgcaag tgaaaagcca agtaaggggc gttgtttcat cgtggaaatt
     8041 cctttttcta ttctatgttt ctatatttta gaaagattga gatacaaaaa aaccccgcca
     8101 ttggcgaggt ctcttattga ttttgaaatg attagtcagc aatgatgctt acatatttac
     8161 ggcttttctc gcctttcact tcaaatttaa ctttaccgtc agcagttgca aataaagtgt
     8221 ggtctttacc catacctaca tttgaacctg cgtggaattt agtaccacgt tgacgaacga
     8281 taatgctacc tgctaataca gattcaccac cgaaacgttt aacaccaagg cgtttagctt
     8341 cagaatcacg accgttacga gttgaaccac cagctttttt agttgccatc ttctagatct
     8401 cctctgaaat tatgcttgaa tcccagtgat tttcacttct gtgaaccact gacgatgacc
     8461 ttgttgttta cggctgtgtt tacgacgacg gaacttaacg attttaactt tctcgccacg
     8521 accttgtgct acaacttcag ccactacttt tgcacctgct acaaccggtg caccgatttt
     8581 aacatcttca ccgttaacga ccattaacac tgagtcaaat tcaactttct caccagttgc
     8641 aagttcaagt ttttctaaac gaactacttg accttcactt actcggtgtt gtttgccgcc
     8701 actttggaaa actgcgtaca tatttactcc gctatgtgcc aactgctttt gcttttaggc
     8761 actaaattaa ttcataaaaa tagggttgca attctacgga aaaagattcg aaagagcaag
     8821 agcgattttg agaatttttt gaaatttgca aaaaatggta aaaatactac cgcttgtttc
     8881 aactgcgact aaaaaacaat agactgctcc gattttacaa caaaatcata attctttttt
     8941 gaacacaggc taaattaagg tagaatctac tcaattttcg tcttacttta tggattcgat
     9001 ggctgtgcaa cttttttctt tagaacaaat tcaagccctt gcgaatgaag atatgcaagc
     9061 ggtaaatgcc gaaattttag ctcaacttaa ttccgaagtg ctactgatta accagctcgg
     9121 acattatatc atcgcaggcg gtgggaaacg tattcgcccg ttaattgcgg tgcttgcggc
     9181 tcgtgccttg ggttatcgtg gtaaaaagca tatcacggcg gctgctttta ttgagtttgt
     9241 gcatactgct acacttttgc acgatgatgt agtcgatgaa tccgatatgc gtcgtggcag
     9301 accaaccgca aacgcccatt ttggcaatgc ggctagtgta ttggtagggg atttcatcta
     9361 tacccgttcg ttccagatga tgacggattt agactctctg cgtgtgttaa aagtgatgtc
     9421 tgccgccacc aatgcgttgg cagaagggga agttcagcaa ttaatgaacg tgaacgaccc
     9481 aaacaccact gaagcgaact atatgcgggt catttacaat aaaaccgccc gcttgtttga
     9541 agccgccact caatgcgtgg cgattgcggc tgatgcttct gaggaaattg aaaccgcatt
     9601 aaaaaattac ggttgctact taggcacggc attccagctg attgacgata ttttagacta
     9661 ctccgctcaa agcgaaagtt taggcaaaaa cgtaggcgat gactttgtgg aaggtaagcc
     9721 aaccttgcca ttgctccacg caatgcacgc tgccgacaaa gcaggcaaaa ctactcaagc
     9781 agagctgatt cgcaatgcta tcgaaaacgg cggcgaattg gaacaaattc cacaagtatt
     9841 ggcaattatg caagaacatc aatctctgga ttatgccatg caaaaagccc gtgaagaagc
     9901 ccaaaaagcg gtggattcat taggggtttt accggaaagc gagtacaaac aagccttggt
     9961 ttcattggcg tatttatcgg tggatcgctc ttattaattt gcaaaaaatc atataaattt
    10021 aaccacttgt aggggcaaat gatatttgcc ccataaccta taattctttt gggcaaatgt
    10081 tatttgcccc aacataacaa tgactgaaca aaaccaacga ccccaagaac atattgaaag
    10141 cgtacgagcg agacgccgtg ctcaaaatca gaaaaaaggc ccgaatgccc cgtttgtgcg
    10201 tgagaaatta acgctgccaa acggacataa caaattactg ctgcactctt gctgtgcccc
    10261 ttgctcgggt gaggtaatgg aggctattca cgcctccggc attgagttta cgatctattt
    10321 ttataacccg aacattcacc cgctgaaaga gtatttgatc cgcaaggaag agaatattcg
    10381 ctttgccgaa aagtggggta ttccgtttat cgatttagac gatgactacg aaaacgaccg
    10441 caaagagtgg ttcaaaaaag ccaaaggaat ggaatgggag ccggaacgag gcattcgttg
    10501 cacaatgtgc tttgatatgc ggtttgaaaa agcagcggaa tacgctcaca acaacggttt
    10561 ccctgtattt accagttgct taggcatttc ccgatggaaa gatatgaacc aaatcaacga
    10621 ctgtggtcac cgtgcagcag ccaaatttga tgacgtggtt tactgggact acaactggcg
    10681 taaaggcggc ggctcacaac gaatgatcga aatcagcaaa cgtgaacgtt tctaccagca
    10741 agaatactgc ggttgcgtct attcgttaag agatagcaac aaatggcggt tggaaacagg
    10801 gcgtcagcgg attgaaatcg gtaagctcta ttactcaccc gattaaatgt tacttttctt
    10861 tgcttcgcca aagaaaagta accaaaagaa aggcgacccg aacttcgctt agttacgatt
    10921 tccttaacgg aaaatttgta aactcgctac gctcaaacag tacaaatttt cctagaaatc
    10981 gcaagtcgct cagacgggac aagcggtaca ttattcaaaa ttttttacaa aatatagtga
    11041 tgtactcttc ccttctgaac cgcccgaacg tgtttgaatt ggtaggaaaa tttactctga
    11101 gcgaagcgaa tgtaaatttt ccgtgaaacc aattcaaact aagtgaggat aagcggtgaa
    11161 gtaggggtgt ttctttggtt actttctttg cacatgcaaa gaaagtaacg aaaactcaga
    11221 tatagatttc aacttaaaaa tttacagaag gaataataac aaatgtcaaa attcgctatg
    11281 gttttccccg gtcaaggctc acaagcggtt ggaatgcttg ccgaccttgc gccacaatat
    11341 ccaatcgtgg aagaaacctt caaacaagca agtgatgtgc ttggctatga cttatgggat
    11401 ttagtgcaaa acggtactgc cgaagagtta gggcaaaccc accgcactca acctgcgttg
    11461 cttgcctcaa gcgtggcact tttccgcatt tggcaacaaa aattccctga gcaaaaacca
    11521 gccgtgatgg cagggcatag tttgggtgaa tactcagcgt tggtgtgtgc cggtgtgttg
    11581 gatttccaag atgcgattaa attggtcgag ttgcgtggtc gtgcaatgca agaagccgtg
    11641 ccggcaggca gcggtgcgat gtttgcgatt atcggtttag ataacgaaag cattattaaa
    11701 gcgtgcgagc aagcagcaag cgagaccggt gaaattgtct ctgcggtaaa ctttaacagc
    11761 ccggggcaag ttgtgattgc cggcacaaaa gcagcggcag aaaaagcagg agaattgtgt
    11821 aaagccgcag gtgcaaaacg tgcgttacca ttagcggtga gcgtaccttc acactgtgct
    11881 ttaatgaaac cggcggcaga aaaattagcc gaagccttgc aaaatattga gctaaatcaa
    11941 ccgcttgtcc ctgttatcaa caacgtggat gtggcagtgg aaacagatgc cgataccatt
    12001 cgtaatgcac ttgttcgcca actttacagc cctgttcgtt ggacggaaac cgttgaaaaa
    12061 atggcacaag atggcatcac tactttatat gaaatcgggc caaacaaagt cctaaccggt
    12121 ttagcaggtc gtattgtaaa agaattatca gccaaagcgg taaatgatgt tgccagcttt
    12181 gaggcggttg aggcttaatt ttcgggagcg taaaatgagt aaagaaaccc tatttatgct
    12241 aataagtgca ttgattgcct tagtgatttt acctttagct tatactgttg gtatcgctaa
    12301 agaaacatta ggcggaaata tgggaatgtt atccattgct gccggttgct catttttagc
    12361 ctatttttta agtctaaacc cgaaaattcg ccacgccttt atcggaggtt cagctattac
    12421 agctgctgct attcaagctt gggtattata tggtgtgcta ttaagcgaaa atgaccccaa
    12481 aagtgcggtc gttttatttc aaatttatct gtttagtagt ttagggccac tgtttttttc
    12541 agctatttgg gcatttactc gcccacgttg gatttaccct aaacaaggta gtgcggtgaa
    12601 atatgcctta tttggtggct taacgaccgt gataaactac ttgattaccc tttttatttt
    12661 ttatattctg acgttataac gtcatactaa ggagaattta tgagcaaaat cgcattagtt
    12721 acaggggcaa cccgtggaat cggcaaagcg attgccgaag aattggtagc aaaaggctac
    12781 atcgtagtcg gcaccgcaac ctctgaaaaa ggggcggaat ctatttcagc ttatttaggc
    12841 gagaacggca aaggcttagt gctaaatgtt gccgatgcag agtcgattga gagcgtatta
    12901 aagcagatca aagagacctt tggcgatgtg gacgtgttag tcaataacgc cggcattacc
    12961 cgtgacggct tattaatgcg tatgaaagat gaggattggt tcgacatcat tcaaactaac
    13021 ttaacttcgg tttatcgttt atccaaagcg gtattacgcc cgatgatgaa aaaaggcgga
    13081 cgcattatca cgatcggttc tgtggtcggc tcaatgggca acccggggca aaccaactac
    13141 tgtgctgcca aagcaggctt agttggtttt tctaaatctt tagcgaaaga agtagcaagc
    13201 cgcggcatta cggtaaacgt ggttgctccg ggctttatcg ccactgatat gaccgatgag
    13261 ctaaccgaag atcagaaaaa tgcgatttta agccaaattc ctgccggtga actcggttct
    13321 ccacaagata ttgccaaagc agtcgctttc ttagccagcg atgatgcccg ttatatcaac
    13381 ggtgaaacca ttcacgttaa tggcggttta tatatgagct aatccgctac aagcggtctt
    13441 ttttcaaccg agctttgtaa attcagagtt cggttttgtt atttgttatt ttgtgttatc
    13501 acctgcttta taggtatagg tgaaaaagtg tgaatatgtt atgactagaa atacattagt
    13561 agtaggcttt ttattgtttg ctattttctt tggtgccggc aatctgattt tcccaccaaa
    13621 attaggttat gaaagtagta cagaattttt ctcagccatt tcaggctttg ttttaactgg
    13681 agtagggctt cctctactcg gtattatcgt cagtgcattt tataatggag gctataaatc
    13741 tgcattagac aaaatccacc cttggttttc tttagcattc ctaacttcta tctatttagc
    13801 gatcggtcca ttctttgccg gtccgcgcac ggcagcaact gcctatgaaa tggcgataat
    13861 gccgttttta ggggattcca gccaagtgtc aatgttaatt tttaccttaa tttactttgc
    13921 ctttacccta tggttagcgc taaatccaaa taaaatggtt gatcgcattg gtgcaattct
    13981 taccccgatc ttgcttataa ccatcattgg attagttatt cgagccggct ttattttaga
    14041 taccgatcca caaatcacta cgcctaatac gaatgaacaa tcttactttt ttaaaggtat
    14101 ggttgatggc tatctaacta tggatgcact tgcttcgtta gcctattcgg taattgtatt
    14161 aaatgctatt cgtgggaaag gggtaagcca agccgcatta caacgccaaa cagtttctgc
    14221 cggcttagtt gcagcagttg ctctagctgt aatttatatt tcattgggtt ggattggtaa
    14281 taatatgcca attagtgcaa acacactttc gatgttatca gaaagaaaat tggatctagg
    14341 tacttacatt cttaacactg tgactaccca agccttcggt gaactcggac gtagtgtctt
    14401 aggagtgatt gtttcacttg cctgtttaac cactgctgtc ggtttagccg tatcagtcag
    14461 tgaatacttt aatgaaattt tcccgaaaat ttcatataaa gtttatgcta tcttatttac
    14521 tgtgattagt tttactgttg ctaaccaagg attaaaagaa gttattagca aatccattcc
    14581 ggtattatta gttctctacc caatagcaat gactgtcatt ttaatgttgc tgattaattt
    14641 agtgattaaa cttccgttgt ctgcacaacg tctatcactc ctattagtga caattgtagc
    14701 gatagcttca gttgccggtg tcgggtttac cgaaaacttg cctctaaaat cctactcgat
    14761 ggaatggcta cctttcgcta tcattggagc tattattgga gcaattatcc acccgcttac
    14821 cggtcgaaag cataaataaa gtaaaggcag tgagcattca ctgcctcttt tttaggagag
    14881 aataatgcct gaattacccg aagttgaaac cagcgttcgt ggtgtatcgc cctatttggt
    14941 tgggcaaact attgaacaaa tcatcgttcg tcagccaaag ttacgttgga cggtgagtcc
    15001 tgaattaagc caaatggcag gggcaaaaat taccgcagta gaaagacgtg ccaaatatct
    15061 gattatccgc accgacaaag gcgatatttt ggtgcatctg ggaatgtccg gctcgctcgg
    15121 gattttgcaa gaacgtcaac aaaaagaaat tggcaaacac gaccacgtcg atcttattac
    15181 ccaaaacggc acaattttac gctataacga ccctcgcaaa ttcggctgtt ggctatgggc
    15241 agaaaaagca gaagatcacg aactgttgcg taaactcggg cctgaaccgc tttcaaacga
    15301 tttcacagca gattacttat ttgcaaaaag ccgcaaaaaa acgaccgctt gtaaaacctt
    15361 tattatgacc aatgaagtgg ttgttggggt ggggaatatc tatgcgtgcg aaagtttatt
    15421 tatggcaggc attcaccctg agcttgcagc acaaaacctg actaaaaaac agtgcgaacg
    15481 tttagtaaaa accatcaaag aagtgctgac taaagcgatt attcaaggcg gcacaacact
    15541 gaaagatttt atccaacctg acggcaaacc aggctatttt gctcaagtgt tgcaagtgta
    15601 tggaagaaaa ggggaaacct gtaatgactg cggaaagccg attgaaacta aagtaatcgg
    15661 gcaacgtaat acctattttt gcccgaagtg tcagagattg ccgaagtaat ctaataataa
    15721 actctctccc cctgagggag agagacagct tgttgaaacg ttaagtgaaa caagcagaga
    15781 gagggtaaaa cctaaacctg caaccaaaag gtaacaggcc cgtcattttg taggctgact
    15841 tgcatatcgg ctgcaaattg cccggtttgg gtatgaatct tagtggcggc ttgttgatga
    15901 aagtaggcat acaaacgttc ggcatcaacc gggcttgctc cttttgagaa actaggacgc
    15961 aagcctttat tggtatccgc cgcaagggta aactgtgaaa ccactaacaa actgccacct
    16021 gcttgctgta cgtttaagtt cattttcccc tcgctatccg aaaacacccg ataattcagc
    16081 actttttcca gtagcttatc tgctttcgct tcatcatcgt ctttttccac accgagcagc
    16141 accaacaaac cgcttgcaat ttcgccaacg acttgccctt caacctccac ttttgcgaat
    16201 ttaacccgct gaattaagcc tatcattaat gctccccttg tctgtttcca tttcgtctaa
    16261 aaccgccgca aattgcccgc caagtaatac cacctgccaa cttaactgaa tccataacag
    16321 cataatcggt aacaccgcta atgctccata aattgcctga taagacggga aggcagtaat
    16381 ataccaaata aaggctttct tccctaaagt aaagaaaatc gctgccatta atgccccgat
    16441 agcagcgtgt tgaaatttta cctttctaat tggaataaat ttatacacta aggtaaaaag
    16501 tagccaaatt agcaaaaacg gcatcaattc taataaatgc tgcccgagcg ataacgctcc
    16561 atttccatta aaaatagaaa gcgacattac ataggtgcta atcgcaatgc tagcaccggc
    16621 aagcagcggg ccaaacagca aaatgagaaa gtaaagcaag aaagagaacc acatcggacg
    16681 ctgacgatta tcgtgccaaa tggtattcaa tgcctcatca atactcttaa tcagcataat
    16741 agtaaccaca aataagccga taaaacttac cagccccatt tgtttggcat tactgacaaa
    16801 caactcaatg tattccgaca ccatctcgct cgcactcggg gcaaagttgt cataaatgaa
    16861 cgcttttaac tgctcgtttg cttcgtggaa aatcggaaaa aaggtaaaaa tggaaaacag
    16921 cacgacaata aacggcacta tcgccagcat tgtgctataa gtgagataac cggctgaaat
    16981 cgcaatgtta ttttcattgt aacgcttggc aaagagctta ataaatctcc acagtcgatt
    17041 taacatctta acctctcacc aaataatcgc cttcacacac ccatttgtag gtggttaagg
    17101 cttctaatcc catcgggcct cgtgcgtgta atttctgcgt gctaactgcc acttctgccc
    17161 ctaaaccaaa ctcaccacca tcggtaaaac gggtgctggc attaatataa accgccgcgg
    17221 catcaacctg ttgtacaaat tgacgggcaa gttgataatc ggaggttaaa atcccctctg
    17281 aatgctgcga accataatgg cggatatgct ctaccgcttg ttgcaggtca ttcaccacca
    17341 ccacgttaag atcaagcgat aaccactctt ggctcaattt tgcttgatca agcggttcaa
    17401 ttccttcaat tttttgcaaa tcgttactgt gaatagtaac gccaagctgt tgcattttgg
    17461 cgaccaattt cggcaaaaat tccaccgcaa tcgctttttc caccaacagc gtttccagcg
    17521 tattgcaagt gcttgggcgt tgcgttttgg cattagcgat gacctctaga gatttctcct
    17581 gatccgctgt tttttccaca aacagatgac aaaccccaat cccccccaca atcaccggaa
    17641 tggtcgaatt ttctttacag aattgatgta aacctgcacc gcctcgaggg atcaccatat
    17701 caatgtagcg atctagcttc aatagttcca ataataaggc tcgatccgga tccgtcaccg
    17761 cttgcactgc caatttcggc aagccggctt tttctaacgc atcttgcact acatcaacca
    17821 acacagcatt ggtaaatttg gtctctttac cgccacgcaa aatcactgca ttgccggttt
    17881 ttaggcataa tgatgccaca tcaatggtca cattcgggcg agcttcgtaa atggttaaaa
    17941 tcaccccgag cggcacacgc tggcgttcga ttttcaaacc gctgtctaac acgccaccgt
    18001 ccatcacctg ccccaccgga tccgctaatt tggcaacatt tcgcacatcg ttagcaatgt
    18061 cttttagccg atcttcattc aataataagc ggtcaataat cgccaatgaa atcccttgtg
    18121 cttgagcaaa ttcaatatct ttggcattag cgtataaaat ttcttctgca cgttgctcaa
    18181 ggctatcggc aatcgtgagt aaggcttggt ttttctgtag gttgccaaat tgagccagct
    18241 caaaactagc ttgtttggct tgctgggcaa gttctatcat ctgcataaat taggtctcat
    18301 ttaatcaata ttctccataa tctaacacgt tcttttatgt gaagaaatat gcaaaagcca
    18361 agaaaattgg ataaaatggg ggaattttaa aacattacgc aattcccctc tccctgattt
    18421 ttctcctgaa cggagaaaaa tctatccctc tcctacaagg ggagagggta gaatttaact
    18481 ctctcccctt gtgggagaga gaccgcctac agattcgttc agaatctgta ggcagagaga
    18541 ggggtaaaaa cagataaaac ttaaacaaaa tgataaacac tacccctaaa cagctaatcc
    18601 acattcttcc gcctcaactg gcaaaccaaa ttgctgccgg cgaggtggtg gaacgccctg
    18661 cgtctgtggt aaaggagcta gtggaaaaca gtttagatgc cggagccaat actattcaaa
    18721 tcgacattga aaaaggcggc tctcagctta tccgcattcg agataacggt tgtggtatca
    18781 gcaagcaaga tttaccgctg gcacttgctc gccacgctac gagcaaaatc agcagcttgg
    18841 aagatttaga agccatttta agtttaggct ttcggggcga agcattggca agtatcagct
    18901 cggtttcacg tttaacactg acctctcgta ccgcagagca aaccgaagca tggcaagcct
    18961 atgcacaagg gcgggaaatg gcggtggaaa ttcagcctgc ctcacacccg attggcacaa
    19021 ccattgaagt agcaaatctg ttttttaaca caccggcacg gcgtaaattt ctacgcaccg
    19081 ataaaaccga atttgcccat attgacgagg tggttcgccg cattgccctt gccaagccga
    19141 atatcagttt cagcttaagc cataatggta aaatcgtccg ccaatatcgc aaagtgcaag
    19201 acaattcggt agagcagcaa caaaaacggg ttgcagccat ttgcggtgat gagtttattc
    19261 aacattcaac ttatattgac aggcaacacg gggatctgca cctacacggc tgggttggct
    19321 caccggctct tgcccgtctg caaaacgacc tttgctacag ctatgtgaac gggcgaatga
    19381 tgcgggataa aaccatcaat cacgccattc gccaagccta cggcgacagc attgcaagtg
    19441 gttactatcc ggcttttgtg atctttttgg atttagatcc gacaatggtt gatgtaaacg
    19501 ttcacccggc taaacacgag gtgcgctttc atcaaggaag actggtacac gactttattt
    19561 tgcaaggcgt actcaacagc ctgcaacaaa cccaacctac gcttgaactg cgaaatgaag
    19621 tgaacgagcc gttaccgagc tatgttcaag acactaaccg ccaagctgcg gggcaaaata
    19681 tttttgtgtc gcaaaccgct ccacaagcgg ccgaatttac gcaaaaaaat gcaagtcgat
    19741 caagctatac gcctaaattt gaacgaagca acagtgtgag ttcgtccgct caaaaatggt
    19801 ataaggaatt ggttggtggc gatttgcacc aaacaacgcc aactatcaaa acgcaaagca
    19861 cgcttgttga gccaaaaaca gaaccccaaa ctgaacataa aacagaagcc gtttcgcaaa
    19921 aaattacgca aattgcaccg cttgcaagcc accattcaca agccttagcg attgtaaaaa
    19981 accaagcggt gttaattaaa gaagaggaaa atttttacct gcttccactg gcaacattag
    20041 ccgagcttaa actcaagcta caactggaaa aagggcaatc tcaagcgttg ttgatcccac
    20101 tgaatctcag tttagatcaa caacaaaccg aacgctggca acaaaccaaa gaagaactaa
    20161 ctaagcttgg cttttcgatc agcgaaaaac agtggcaagc ccaaacacgg ctcacagtgc
    20221 tttccgtacc acaggctgtc agagaacaaa acctgcaaca gcttttattt gctctgttca
    20281 accaaacaca agcggtcaat ttagccgaat tttttgcaaa aaaatgcgaa aaacccaccg
    20341 cttgtacgct aagtgacgtg attgctctac tctcagaaat tgagctttat gccaatggga
    20401 aaaaagagct ggagggaata aggaaggaag tggatttttc caagttttta tagtctaaag
    20461 ggcaataccc tttagccaaa taaatgagag tggctgccaa ttcgcagcag ctctagctta
    20521 tcatcttcca cttttcgata gataagaacc aaatcaggtt ggatatggca atccctaaag
    20581 cctttccatt cgccggttaa agcgtgatcg cagtatttct caggtaatgg cttgtcatta
    20641 atcagaaaat acaacgcctc aattaatgaa gaggataaag actcaaccgc acatttttta
    20701 tagtcacgtt taaaagcatt actatactca atctctcgca ttgagttgct ccattaaatc
    20761 cgcaatacta tttgctgaat gtagcttttc ctcgccattc ttaacagccc ttaatacttc
    20821 tgctgtctca aaattaggct gataatcaaa ctgaaaacgt ttttctgttg cgattttcgc
    20881 taatgtcatt cgcatcacat cagagacagt caagccgata ccggctaaca cctcacttgc
    20941 ttgctcttta atttcagggc tgattcttac gcttactaat gcactatttg ccatcaaaaa
    21001 ctccttttgt atatcaaatg atatacaa
//
LOCUS       DANLXQ010000025        20877 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.025, whole genome shotgun sequence.
ACCESSION   DANLXQ010000025 DANLXQ010000000
VERSION     DANLXQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20877
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.025"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..400
                     /gene="secE"
                     /locus_tag="PXD94_000731"
     CDS             <1..400
                     /gene="secE"
                     /locus_tag="PXD94_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL6285622.1"
                     /translation="KTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAYFSSHF
                     SLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQTTLIV
                     LAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            402..965
                     /gene="nusG"
                     /locus_tag="PXD94_000732"
     CDS             402..965
                     /gene="nusG"
                     /locus_tag="PXD94_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL6285623.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            1198..1626
                     /gene="rplK"
                     /locus_tag="PXD94_000733"
     CDS             1198..1626
                     /gene="rplK"
                     /locus_tag="PXD94_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL6285624.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            1631..2320
                     /gene="rplA"
                     /locus_tag="PXD94_000734"
     CDS             1631..2320
                     /gene="rplA"
                     /locus_tag="PXD94_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL6285625.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            2382..2801
                     /locus_tag="PXD94_000735"
     CDS             2382..2801
                     /locus_tag="PXD94_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285626.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            2888..4110
                     /locus_tag="PXD94_000736"
                     /pseudo
     CDS             2888..4110
                     /locus_tag="PXD94_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            4103..4507
                     /locus_tag="PXD94_000737"
     CDS             4103..4507
                     /locus_tag="PXD94_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL6285627.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQAGEPVRLGYLLGTRKLHLHRQEIPIGSQLQIQIKMSIQ
                     DATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            4607..6040
                     /locus_tag="PXD94_000738"
     CDS             4607..6040
                     /locus_tag="PXD94_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL6285628.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRVITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQYLYENEMITVKIASEILDRSKELSRRIL
                     KSLEQKNILTWHGLSPKDPTQYYTLKK"
     gene            6054..6782
                     /gene="fabG"
                     /locus_tag="PXD94_000739"
     CDS             6054..6782
                     /gene="fabG"
                     /locus_tag="PXD94_000739"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL6285629.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKATYITRQVIAVNGGLC"
     gene            6845..7990
                     /locus_tag="PXD94_000740"
     CDS             6845..7990
                     /locus_tag="PXD94_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL6285630.1"
                     /translation="MKRVVITGIGVVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDRRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASKVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGVNTSLIFKRWK"
     gene            8004..8909
                     /locus_tag="PXD94_000741"
                     /pseudo
     CDS             8004..8909
                     /locus_tag="PXD94_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
     gene            8932..10074
                     /locus_tag="PXD94_000742"
     CDS             8932..10074
                     /locus_tag="PXD94_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL6285631.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQHRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            10123..10338
                     /locus_tag="PXD94_000743"
     CDS             10123..10338
                     /locus_tag="PXD94_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL6285632.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            10619..11110
                     /gene="rplJ"
                     /locus_tag="PXD94_000744"
     CDS             10619..11110
                     /gene="rplJ"
                     /locus_tag="PXD94_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL6285633.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            11169..11537
                     /gene="rplL"
                     /locus_tag="PXD94_000745"
     CDS             11169..11537
                     /gene="rplL"
                     /locus_tag="PXD94_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL6285634.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(11644..12474)
                     /locus_tag="PXD94_000746"
     CDS             complement(11644..12474)
                     /locus_tag="PXD94_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6285635.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            12700..14241
                     /locus_tag="PXD94_000747"
     CDS             12700..14241
                     /locus_tag="PXD94_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL6285636.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENITPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            14244..15032
                     /locus_tag="PXD94_000748"
     CDS             14244..15032
                     /locus_tag="PXD94_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL6285637.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            15045..16302
                     /locus_tag="PXD94_000749"
                     /pseudo
     CDS             15045..16302
                     /locus_tag="PXD94_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(16381..18390)
                     /locus_tag="PXD94_000750"
     CDS             complement(16381..18390)
                     /locus_tag="PXD94_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6285638.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAAREHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLPNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNSKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNTAKTETKS"
     gene            complement(18400..19737)
                     /gene="rimO"
                     /locus_tag="PXD94_000751"
     CDS             complement(18400..19737)
                     /gene="rimO"
                     /locus_tag="PXD94_000751"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL6285639.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEATKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMADAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
     gene            complement(19892..20584)
                     /gene="mtnN"
                     /locus_tag="PXD94_000752"
     CDS             complement(19892..20584)
                     /gene="mtnN"
                     /locus_tag="PXD94_000752"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL6285640.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVSGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLAMLNNID"
     gene            complement(20642..20863)
                     /locus_tag="PXD94_000753"
     CDS             complement(20642..20863)
                     /locus_tag="PXD94_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL6285641.1"
                     /translation="MDNNYTLSAKITELETKVAFQELIIEELNQALIEQQFAIDKLQT
                     QIRHIAEKLKGAQLSQVASQAEETPPPHY"
BASE COUNT         6098 a         4273 c         4666 g         5840 t
ORIGIN      
        1 taaaacaaaa acccccgagc aagtcgaaca aaaagggggg aaaagcaaag gtgcaaatac
       61 agcattatgg ctggttgcga ttgtattttt agcagttgct gcggtaggta atgcctattt
      121 ttcatctcat ttttcattaa ttgttcgagt gcttttattg gtggtgttgc tagttggtgc
      181 tgttgcacta gcggcaatta caaatcaggg gcaaaaggca attcatttta ttaaagagtc
      241 tcgaaccgaa ctacgcaaaa ttatctggcc gacacgccct gaagcaacac aaaccacctt
      301 gattgtattg gcaatgtgcg tagtagtttc cttggtttta tggggtattg actcaatcat
      361 cgtcgcatta attacatttg taacaagttt aaggttctaa aatgagtgaa gtagaagtga
      421 aagaaaatgg ctcaaaacgc tggtatgtat tacaagcgtt ttcaggcttt gaaaaccgtg
      481 tggcagtaac attgcgtgaa tacattaagc ttcacaaaat ggaagagcag tttggtgaag
      541 tattagtgcc aaccgaagaa gtcgttgaaa atgtgggcgg taaacgccgt aaaaccgagc
      601 gtaaattctt cccaggttat gtattggttg aaatggaaat gaatgacgat acttggcatt
      661 tagtgaaaag tgtgcctcgt gtaatgggct ttattggtgg tacggcagat cgccccgcac
      721 caattaccaa acgtgaagca gaccgcattc taaaccgtgt acaagaaaca gcggaaaaac
      781 cacgccacag aaaagaattc caaccgggcg aaagcatttt ggttacagaa ggtccgtttg
      841 cagatttctc cggtacggtc gaagaagtgg attacgaaaa aggtcgctta aaagtatcgg
      901 tgtcgatttt tggtcgtgcg acaccggtag aattagaatt cagtcaagtg gaaaagcaga
      961 gctaatttca attaatttca cacacaagcg gttatttttc tcaatttttt gcaaaattca
     1021 gcggaaaaaa gaccgcttgt aacaagattt attcaacaaa gcgaaagttt tgcttgaaaa
     1081 atagtcgcaa attctttaaa atttgcgacc tattacgagc cagcctgctg actcgtattt
     1141 tttatgtaac aggggagctg attttcagcg ttatcaccca tttagaggaa aattaaaatg
     1201 gcaaaaaaag tccaagccta cgttaaactg caagttgcag cgggtatggc taacccatca
     1261 ccaccggttg gtcctgcatt aggtcaacaa ggtgttaaca ttatggaatt ctgtaaagca
     1321 ttcaacgctc gtactgagag cttagagaaa ggtttaccga ttccggttgt tatcacagtt
     1381 tacgctgata aatctttcac attcgttacc aaaactccac cggcggcagt gctactgaaa
     1441 aaagcggtag gtatcaaatc tggttctggt aaaccgaaca aagataaagt gggtacagta
     1501 actcaagagc aaatccgtca aatcgctgaa actaaagcag cagatatgac tggtgcgact
     1561 atcgaaacca aaatgaaatc aatcgctggt acagctcgct caatgggctt aatcgtagag
     1621 gaataataca atggctaaat tgactaaaaa aatgaaagca attaaagctg gcgtagattc
     1681 tactaaagct tatgacatca acgaagcagt tgaagtgtta aaaaaattcg caaccgctaa
     1741 attcgttgag agcgtggacg ttgctgtaaa cttaggtatc gattctcgta aatcagacca
     1801 aaacgtgcgt ggtgcaaccg tattaccaca cggtacaggc cgtaccgctc gtgttgctgt
     1861 gtttacacaa ggtgcaaacg cagaagcagc aaaagcagcg ggtgctgact tagtgggtat
     1921 ggaagatctt gcagagcaaa tcaagaaagg cgaaatgaac tttgatgttg ttatcgcttc
     1981 tccagatgca atgcgtgttg taggtcaatt aggtcaagta ttaggtccac gcggcttaat
     2041 gccaaacccg aaagtaggta cagtaacacc aaacgttgca gaagcggtta aaaatgcgaa
     2101 atctggtcag atccgctacc gtaacgacaa aaacggtatt atccacacta ctatcggtaa
     2161 agcagacttc tcagctgaac aattaaaaga taacttaaac gcattgttag ctgctttaac
     2221 aaaagcaaaa ccaacaacag caaaaggtat cttcatcaag aaagtaagca tctctactac
     2281 aatgggtgct ggtgttgcgg ttgatcaagc atcactttaa tttttaatag aatcaaaaga
     2341 ccgtagtgtt aatactctac ggtctttttc tttaggtacg aatgttaaga ttattatctt
     2401 tttgctttat ttttttgctt agtgcttgta ctgtattacc tcaaattaag caattaccgc
     2461 cacaggttga atctgtaaaa tcctttaaag ttgaaaataa tggccagcaa agtttgcttc
     2521 taattcaatt ttatgaaaat aaatggcgtt ggattcaaac tgatccttta ggtgctccta
     2581 ttgccagagt atggcttact aaaaaaggtt gggagccaga tggctttgta atgccaaata
     2641 agcaggcaca gctattattt acagctttag tcaatgcatt aaaccctcat tctccacctt
     2701 tttatttaga taaaggttgg aaaattgaga gggaaaaatc ccaatttacg atttggctac
     2761 cagatagcag taaatggaaa gtggaaatat tgggggaata agcgaaaact tgaacctcta
     2821 tgcttgaatc acttgaatat gaattttata attttagtgc cattacacaa taatactgga
     2881 ataaataata atgacactat atttatcaga gcccgccatc accagtccga ttggatatgg
     2941 catcgtggag catttgaatg ttttaattaa tgcgaaagta tctcctttag atacgacttc
     3001 acagccttat ttacgacata atatttcaaa tagttcaccg aaatttctag ggcaagtctc
     3061 tgctagattg cgtgattttc ctgttgaact atctattgaa caccgcagcc gcaataacca
     3121 actgctatgg cacgcccttg cagaaattga gccgaaaatt accgcttgta tcgagcaatt
     3181 cgggcgtgag cggattgcgg tgatcatcgg cacgtccact accggcgccg atgaaaatat
     3241 tcccgtcttc aaagccggtg tgcagggcga ttatgccacc gagcagttta atcaacagca
     3301 aattctcttc tctgcgccgt ctgatttcgt tgccgaagtg tatggcttaa cggggctgag
     3361 ttacggcatt tccaccgcct gcacctcaga ggcaagagcg ttaatttctg ctgcccgttt
     3421 actgaaagcc ggcttgtgcg atgcggtgat ttgcggtggt gtggataccc tttcgccttt
     3481 aactatcagc ggatttagct cgctggaagt gttatcaaac caacaaacca acccattttc
     3541 cgctaaccgt aacggcatta acatcggcga aggcgcagcg gtgtttgtga tgagccgtga
     3601 accactggac gaacatcgca ttcagttact gggttatggt tccagctcgg acgcttacca
     3661 tatgtcctca cctcaccccg aaggggaagg ggcaatttcc gctttccaaa atgccctgca
     3721 caatgctgat ttagacgcag ccgacatcgg ctggatcaac ctgcacggca cagggacgct
     3781 tcataacgat caaatggaaa gcctcgctgt ggcaaaaatc ttcggtaatc agaccgcttg
     3841 caccaccacc aaaccctaca ccgggcatac ccttgggctg ccggtgcggt ggaagcggca
     3901 attttgtggg gaatgctctg tcgccaacac aacccgaccg gcaagctccc tgcgcaatta
     3961 tgggataaac aagcggacga aaacctgccg aacattgcga tcaccgatga acagagccgt
     4021 tgggcagcag gcagacggat tggcgcaagc agctcttttg cctttggcgg caataacagc
     4081 gtactgattt tgggagaaag cgatgcataa attcaccttc cctatcgaac aaattgcacc
     4141 attggttcct cacagtggca atatgatcct actcgaccgc attaccgcat tcggcgagga
     4201 ttttttgacc gctgaaacca atattcgccc tgacaatccg ctgatcaaac acggaaaact
     4261 tgccacctac gccagcattg aaattatggc acaagccggc gaaccagttc gcttaggcta
     4321 cctgctcggc actcgcaaac tgcacctcca ccgccaagaa atcccgattg gctcacagct
     4381 acaaattcag atcaaaatgt ctattcaaga tgccaccggc ttcggcgtat tcgactgcca
     4441 actgatcgat ttagccgaca accaagtgct tttagaaggg gcgctgaatg tgtttagtcc
     4501 gaaatagagt tggtagtagt aagtagtagt aagtagtagt aagtagtagt aagtagtagt
     4561 tggtagtagt tggtagtagt tggtagtagt tggtaggaaa ttcgttatgc aagaggccta
     4621 tgacattcaa catctttgta ctcgcaaaga agataaatcc tttgatcgca aaagtgcgag
     4681 aaaagaaccg aaagagattt tgcggcattt gattggtttt gcgaatgcag acggcggttt
     4741 attagttatt ggtattgaag ataatggcga aattaccggt tttaactacc aaaaagccca
     4801 tcagccggaa gaatttatcc acgctctgca caatttacaa aaaatgccga taccggtgac
     4861 ctatcagcaa attgaggtgg taaatagtcg aggtttagcg gattttgtct tattatttga
     4921 agttacagct tcgagtggga gggtgattac ctctcacgat gatcgggttt ttctacgctc
     4981 aaaagatcaa actttggaac tcaattttga acaacgtttg caattagagt acgaaaaagg
     5041 gcagcgtgct tttgaagagc aaccgatagc acgcgctgat tttactgatc tggatatgga
     5101 tttattagaa cagtatcgac agaaattaca cacagatcgc tcaatagaag agattctgaa
     5161 agcccgagat ttaattaaca aagaagggca aattacgaat gcctgtattt tgctatttgg
     5221 gaataacccg acacgttatt ttcctaatgc ccgtttgcgt tttttgcggt atgaaggtaa
     5281 agcacctctg accggtagaa cttttaatgt cgtcaaagaa tttatgcttg aagagcctat
     5341 tcctaagtta ttggagagtg ctaaggcagt attaaaaagc caattacgag agttccaacg
     5401 tttagatgaa aacgggcaat tcacaaaaat tgatgagtat ccagaatttg cttggttaga
     5461 aggtattgtc aatgcgattg cacaccgtag ttacagtaat caaggggact gcattcgcat
     5521 ttcgatgttt gatgaccgtt tggaaatttt tagtccgggt ggattacctc gccctgtgac
     5581 gcttgaaaat atgtactata cccgtttttc acgcaatccg aagattgcac gagtgctgta
     5641 tgaatttggt tgggtcaaag agctgaatga aggtgttaat cgtatggcgg aggaaatgaa
     5701 cgcttaccaa cttaatacac cttcttactt tgaacccaat gaaaatgcgg tcagactgat
     5761 tttagaaaat aatattgaac atcggacatt aagggcatta gaacgaatag aagaaatttt
     5821 tacgcaggat attatggcga cattaaataa aaatgaagtt actatcctgc aatatttata
     5881 tgaaaatgaa atgattactg taaagattgc tagtgagata ttagatcgga gtaaagaatt
     5941 atctcgtaga atattaaaaa gtttagaaca aaaaaatatt ctcacttggc acggcttaag
     6001 tccaaaagat ccaacccaat actacacact caagaaatag taggaaatat aagatgaata
     6061 atacagttct catcaccggc tcaagccgtg gtatcggtaa agcgattgct ttaacccttg
     6121 cacaacaagg gtttgatatt gtggtacatt gccgttctcg tgtggaagaa gcggaagcgg
     6181 tggcacaagc ggtcagaaat ctcggaaaaa atgccagagt gttacagttt gatgtggcgg
     6241 atcgtgaaaa agtgcggtcg attttagagg cagatgtgga acaacatggt gcctattacg
     6301 gcgtggtgct aaatgcaggg cttacccgtg ataacgcctt tcctgcttta accgatgagg
     6361 attgggatag cgtgttgcgt accaatttag acggttttta taatgtcctg cacccgatta
     6421 tgatgccgat gatccgtcgc cgtaaagcag gcaggattgt ctgtatcacc tcggtttccg
     6481 gcttgatcgg caaccgtggg caggtcaatt acagcgcttc aaaagccggc attattggtg
     6541 cggcaaaagc actcgccatc gagcttgcca aacgtaaaat cacggtaaac tgcgtggcgc
     6601 ccggcttgat tgataccgat attctggacg aaaacgtccc aatcgaagaa attctcaaaa
     6661 tgatccccgc aggacgaatg ggcgatcctg atgaagtggc acacgccgtt gcgtttttaa
     6721 tggatgaaaa agccacttac atcacacgcc aagtcatcgc tgtgaatggt gggttgtgtt
     6781 aactttagga taataattcg taggggcgga tccagtaccc gcccgaagcc aaaaagagat
     6841 aaatatgaaa cgagtcgtca tcaccggaat tggtgtggtt accgcttttg gtaaagagtg
     6901 ggcggaaatt cacggctatc aaccgccgaa acattggaac cgcaagcagt tgcgcagcct
     6961 tgggcgagtg tcgcaatttg tggtggaggc cagcgagcgg gctttagcac aggccggttt
     7021 gttggacgaa aacggcgtga ttttcgactg gctaaaagac aggcgaatgg gcgtggcgtg
     7081 tggctcgtcc acaggctcta ccaatgacat caaagatgcc gccgaattgc tgatgaccgg
     7141 caaatcggac ggtttcaatg ccaacaccta tgtgcgaatg atgccccaca ccaccgctgc
     7201 caatattggc attttcttcg ggctgacagg gcggattatc ccgacttcca gcgcttgctc
     7261 ctcgggcagc caaggtatcg gttacgctta cgaagccatt aaatacggta tgatcccgat
     7321 gatgcttgcc ggcggtggag aagaattttg tgcctccaaa gtctatgtct tcgacagcct
     7381 ctatgctgcc agccgccgca acgacaaccc aaaaggcacg ccacgcccct atgacaaaga
     7441 ccgagacggc ttagtgatcg gcgagggagc gggaatgttc gtattggaag agctggaaca
     7501 cgcgttagca cgaggggcaa acattatcgc cgaagtggtg ggctacggtg caaacagtga
     7561 cggttcccac gtcacccgcc cacaagccac cacaatgcaa cgctgtatgg aacttgccct
     7621 taaagatgcc ggtattcaac cgaaccaagt cggctatgta aacggacacg gcacggccac
     7681 cgagcagggc gacattgccg aaactcaagc caccacagcg gtgttcggca aaattccgct
     7741 cagctcgcaa aaaagctact tagggcatac gctcggtgcg tgtggtgcat tggaaagctg
     7801 gttcagcatt gaaatgatgc gagacggctg gttcgcccca acactgaatt tagaaaatgt
     7861 cgatgaacgc tgtggcgact tggattacct tcaaggtgac ggacgggaaa tccacaccga
     7921 ttatgtgatg aacaacaatt tcgccttcgg cggtgtgaat acctcattga tttttaaacg
     7981 ttggaaataa ccgttttcaa ataatgaaaa agatattatt agtttattac tcccaaaccg
     8041 ggcaactcac ccatttggca gaaaattttg tacaatcgct tgaacaagcg ggcgtttttg
     8101 tggaaaaact cgcaatcaaa ccgcaacagg aatacccgtt tccgtggcgg tttatgcgtt
     8161 tttttaacac cttccccgaa accgtccacc tcacaccgcc gccgattgag cctctgccct
     8221 ttcagcacga aatatacgat ttggtgatca tcgcctattc cgtgtggttt ctctccccca
     8281 gccagccgat taccgctttt ttacagagtg aacaggcaaa aaagtgttga aggatacgcc
     8341 cgtcatcacg ctgatcggct gtcgcaatat gtggctgatg gcgcaggaaa aagtgaaaaa
     8401 actgcttgcc gagagtgagg cgaagctcat tggcaacgtg gtcaaagtgg atcaatctaa
     8461 cgactgggcg agttttatca ccacaccgct ttggatgctg accggcaaga aaaaaaatcg
     8521 gcaagctgcc gagcgctggc attgccgaaa gcgaaatgga agatgtaaaa cgcttcgggg
     8581 caaaactttt gcacattttt agccaaaacc aaccgcttga cagcaccctt ttccgccaaa
     8641 tgcaagcggt caaaattgac gaaaaattga tgatgagcga aaaagtcggt gcgagaagtt
     8701 tccacctttg gggcaaatta ctgatcaagt gcggtcaaat ttcaccgcac ttacgccaag
     8761 cggtactcta tttttacatc ctctttttag tgacgttaat tttaaccgtc gtcccgattt
     8821 caacggtgat taaacggcta ttacgcccac tgctgaaaga aaaattagaa aaacaaaaac
     8881 ggtattttgc cgagccttcg ggcgagtaaa cgtaccctac acggaaactt tatgctatct
     8941 aacgtctata tcaccaacac ctccgccttt ttacccaacg cccctgtgtc caacgatgca
     9001 atggaagcgg tgttgggaat ggtgggggaa acgccttccc gagtgcggaa gatgattttg
     9061 cgttctaacg gtattcaaca tcgttattat gccattgacc cgataacccg ccaagcgact
     9121 cacagcagta ccgagttggc ggtgcaagcg gtcaaaaaat tgggaaattc tgcaacatca
     9181 gcgacttgct tggcggtggg gacatcttac cccgaccaaa ttatgccggg gcagggggtg
     9241 atggtacacg gcttgcttga tgccgaaccg atggaagtgc tgaccactgc cggtgtgtgc
     9301 gtggcgggaa tggcagcgat gaagcacgcc tataatgccg tacgcatcgg tgagcatcgt
     9361 gctgcgattg caacggcttc cgaaaccgcc tccgccatta tgcgcagtga acatttccgt
     9421 gccgaaatcg atcataaaaa actggacgat gctcgccctg agatcggttt tgagaaagat
     9481 ttcctgcgtt ggatgctctc agacggtgca ggggcggtat tgcttgccaa tcagccgaac
     9541 gaaaaagggt tgagcctcaa aattcactgg atcgatctgg tctcttacgc taacgaaatg
     9601 ccggtttgta tgtacgccgg tgcggaattt attgacggca actacaccgg ctggaaagca
     9661 atgagcgaag cccaacgtgc cgaatgctcg gcaatggcgg tgaaacaaga tgtcaaactg
     9721 ctcaacgaaa acatcgtcaa atacacggtg gaaaaagcat tggagcggat tatcccgaaa
     9781 tacaagctca acgctgaaga gattgactac ttcctgccgc actactcgtc cggttccttc
     9841 cgagataaat tattggaagg tttacagcgg atcaatttcc tgatcccaca ggaaaaatgg
     9901 ttcaccaacc tgactaccaa aggcaacact gggtcagcct cgatctacat tatgctcgat
     9961 gaatttatcc gcacccacga tctcaaacag ggacaaaaaa tcctctgcta tgtgccggaa
    10021 agcggacgtt tttcgtcttg ttttatgctt ttggaagcgg tggtaaattc gtaaaatcgt
    10081 agggacgggg gctattcccg tccaacaatc atcggaaaac gtatggacat caatgacatt
    10141 ccccaagaca acagcaaaat cttcaacggc gaacgcaaag tgatctacgc ccgcaatccg
    10201 caaccagaaa tccgccaaat gatctcctct attctcgctt gctacgcttg ggacgaaaac
    10261 aacccgtttg tagcgaagtc tgatgcacgg ttgaaggcat tggcggaagt tgttggtaaa
    10321 gcagaaattg gcgattaagt ttgattttgt gctttacagg gtcgcagatt atctgtataa
    10381 tttgcgacct taacttgcga gagcaagctg atggtgctta gcctatctaa gccccgtcta
    10441 agactgtagg ggtgaaaact taataatcct acatagatgg tgaacagaca gaattctctg
    10501 cttctggaca ccttaggctc aaagaagatt gcagtttgta gcaatgcaag cggtaatatt
    10561 tttgggattt tttgtaaatt ctgcttgaaa aggcagagtg tatcaggagc taaaaccaat
    10621 ggcattaaat cttcaagaca aacaagcgat tgttgctgaa gtaaacgaag ctgccaaagg
    10681 tgcactttca gctgttgttg cggattctcg cggtgtaaca gttgaaaaaa tgactgagtt
    10741 acgtaaagca gctcgtgaag ctggtgtgac aatgcaagtt gtacgtaata ctttattacg
    10801 tcgtgcagtg gaaggcaccg agttcgagtg cttaacagat acgtttaccg gtccaactct
    10861 tatcgcattc tcaaatgaac acccaggtgc agcagcacgt ttattcactg aatttgcgaa
    10921 aacaaacaaa gagtttgaac ttaaaggtgc agcctttgaa ggtaaaaaac aagatgttgc
    10981 attcttagca accttaccaa cttacgaaga agcaattgca cgtttaatgg gcacaatgaa
    11041 agaagctgcg gcaggcaaac ttgttcgcac tcttgcagca ttacgcgatc aaatggaagc
    11101 agcagcttaa ttttaagcac tcttctttat tcgtttaaca acttatttta ctttaggaat
    11161 cgattgttat gtcattaact aacgaacaaa tcattgaagc gattgcttct aaatcagtat
    11221 cagaaatcgt tgaattaatc actgcgatgg aagaaaaatt cggtgtttca gcagcggcag
    11281 cagtagcagc agctccagca gcaggcgcag cggcagcaga agagaaaact gagtttgatg
    11341 tagtattagc agaagctggt gcaaacaaag ttgctgtaat caaagcagta cgtggtgcaa
    11401 caggtttagg cttaaaagaa gctaaagact tagtagaatc agctccagct gcattaaaag
    11461 aaggcatctc taaagcagaa gctgaagcac ttaagaaaga attagaagaa gctggcgcga
    11521 aagtagaaat caaataattt ttgatttatt ttgtcagaac ttcaagttca acaaaccccg
    11581 atggaaacat cggggttttg ttttttcaga attgtgtaat gtagatatga ttacctttat
    11641 ccattacttg cgcagcagtg aaaaatgtaa aaattccaca aagtgatctg cccagtgcat
    11701 ttcgtggtgg atttcatttg ccataatccg caggcggata ttgtctaacg gcacgccaat
    11761 ccgaatcagt gcttggtagt aattgagcga acaatcaata taggtttggt tcgtattaga
    11821 gataaagtgc gaatcgattt catcgccttc gttagtgcca acctgaataa acactttggt
    11881 attgggttgt aacggatatt ggtgcgtaaa acgcagaaaa tccggctcac taaaccaaga
    11941 ggcaagcgaa aacaccccta aatgcccgaa aatatgtggg tagcggctgc ccatataagc
    12001 ggtgataatg ccacccattg aactgcctgc caataaggta tgttcttgct gaggcttggt
    12061 gcgatagtga atatcaataa agggtttaac cgtttccacc acccatttgg catattcctc
    12121 ccctaagcct ccggcatttc gggtttcagg ggttgagccg gtggttgttt tccacgggcc
    12181 gtattcattt aagcgattag ctcctgcgtt atcaatgccg acaataatca tcttcggtaa
    12241 ttcttggtgt gatttaatgg ttggaataat tttccaagaa tgccccgaat aagactcttt
    12301 gctgtaaaac acattttgcc cgtcgtgcat atataacacc ggataacgtt cccaaccttc
    12361 gttgtgataa tccttcggca atagtacccg aatgcggcgg tgatggtgta aatagggaac
    12421 atataaaaaa tgctcctgca tttctaaata gtggttaagt ggtttttgta ccatcagtct
    12481 gtccttttgc ttaaattgtg gcgtatttta caccttaatc agtctgagtt aaaacaagcg
    12541 gtcagaaaaa gtcaaaaatt tgcaaattgc tgttacctaa cgcaatcggt tgccttatgc
    12601 ttttgaggaa tataaaagag aaactgtgat ctaagtcaca gcaattttta ttcggtttga
    12661 ctaaaatccg aactatcact taatagagag gaaaatgtta tgcaggcttc attacaagat
    12721 attcttgagg tagtaaaagc aaaaaatctc acttatcagc aaaaattgat gagtttaggc
    12781 aatattgctg agcggttatt taacccgatt gaactacttg gctacactga agaagagtgg
    12841 gaacacattg aaaatcagat gatttgcgat ttaaatgaag gttatgcgat ttatcgccca
    12901 cgttatattt tgcctgatta taatgtgtat atgcaaaaag gttgccaatt cttagattta
    12961 cctccgccaa ccaatttaga tgaagcctta gacggcttgt taattctgta ttcacacgtt
    13021 ccgtccatta ctacttttcc ggtgtatatc gggcgtttag accaactgtt agaaccgttt
    13081 ataaccgatg aagagcaaga ttacattaaa atcaagcgtt tcttaaatca tatcgataaa
    13141 accatttccg attctttctg ccacgctaac atcgggccga atgacaccaa agccggtcgt
    13201 ttaatcttaa aagcggtgat tgagcttgaa aacattacac cgaatatgac gattcgttat
    13261 gataaaacca aaacatcgcg tgaatttgcc gaactggcgg cgaaagcgtg cttgttggtg
    13321 tccaaaccgt cttttgctaa tgatgcctac tacattcaag atttaggcga agaatacgga
    13381 attgcaagct gttataacgc cttaccggaa tgtggcggtg cttacactct gacccgcctg
    13441 cgtttaggta ctatcgggcg tgcttgtgcg agtgttgaac aaatggtcaa tgagatgctg
    13501 ccgaaagtgg caaaactcgc cctttccacg atggataaac ggcataaatt cttggtggaa
    13561 gaaagcaatt tctttgaaag tagcttcttg gttcaggagg gctttatcaa acgtactaac
    13621 ttcacaagta tgattgcgat tgtaggtctt gctgatgcgg tgaatcattt actcaagcaa
    13681 gaaggtttaa atgaaacctt tgggcaaagc aaacgtggcg atgaaattgc gacgctgatt
    13741 atggataagc tccaagcagt aaatgacgct cataaagggc tgtatgttga acgaaccaat
    13801 aaccaatatc tgttacacgc tcaagtgggg gcaaataacc acgctgaaga caaaatgaac
    13861 acgccggcac accgcattcg tgtgggcgaa gagccaacct tattggcaca cttaaaacag
    13921 tctgctcctt tccataaata tttcccatcc ggtacggggg atttattcgc ctttgaccaa
    13981 acctacactg atcatttaga tgcggtggtg gatattattg acggctcgtt cgcccaaggt
    14041 tatcgctaca ttaccactta cctgaaaaat actgatttga ttcgagtgac aggctacttg
    14101 gtgaaaaaaa gcgaggttga acgttatcgc aaaggcgaag cggtattgcg tgataccact
    14161 tggtatggtt caggcaccga tgattgtgcg aaagtgttcg accgccagct gcgtgatgaa
    14221 gaaaatgtga atgcagggta acaatggctg cactttctga gatttttgtg ccgttacacc
    14281 gcattatccc cttctcgaat gtagaagggc agggcaatcg taccagcatt tttctacaag
    14341 gttgcaagct gaattgcctg tattgccata acccggaaac cattccacgt tatagcaagg
    14401 aagcaaaaca ggttagcttg caatacttgt acgatcaagt gatggaggca gttcccttta
    14461 ttcgaggagt cactatttcg ggtggagagc caaccattca tcataaaaaa ttagtgccac
    14521 tgttcgaggc gttgcgtgag caagggctaa cctgttattt agacagcagc ggcttttttg
    14581 attttgaggc aacagagccg ttaattaatg ttaccgataa atttctgttc gatctcaaag
    14641 gagatggggc gggcttacaa acgctatgct ttgatcggaa aaaccaagcc ggcaaagtgc
    14701 ctgaacaggc tcttccaaac gtgaatcata ttaaacctga aaacttggct cgcaatttgc
    14761 aaaatttagc caaattatta ccgcttgata aggtggaaga agtacgtttg gtttacctca
    14821 atgagtttta taacgcccac catttgctcg aaaaagtggc tgacctactt aaaccttacc
    14881 cagatgtact acttaaaatt attcgggtac acaccaaagg agcgagagat gaaacaggct
    14941 tggctcgttt tgtacccact atcgagcaaa ccgatgaact ggcagcattt gcaaaacggc
    15001 acggtattca aaaagttatt actatttact aatggagact tacgatggat atactaatta
    15061 gcataattgg tgtttttgtg ttattaggct taggtgtttt attatcaaat aatcgcaaag
    15121 ccattaaatt tcgcacaatt ttgggcgctt tggcgattca aattggtttt gccgctctta
    15181 ttctttactt tccagcggga agaaatgcat tgctagctac tgcaaattgt gtgagcaata
    15241 ttattaatta tggtaacgaa ggtattagct ttgtgtttgg caatttagcg aatccgagta
    15301 atagcagcat tggttttgtg tttgcggtta aagtattgcc tattattatc ttcttctctg
    15361 cgttaatttc aatgctttac taccttggtg tgatgcaatg ggtgattaaa attattggtg
    15421 gagcattaca aaaaacacta ggcacttcca aggcagaatc aatgtcagcg gcagctaatt
    15481 atttttgttg gtcagacaga agctcctcta gttgtaaaac catacattag ccgtatgaca
    15541 gagtcagaat tatttgccat tatggttggt ggaactgctt caattgctgg ttccgttatg
    15601 gctggttatg ccggaatggg cgttccttta acttatctta ttgcggcctc ctttatggct
    15661 gcaccagctg ggttactatt tgctaaatta atgtatcctc aaacggaaac cttggagagt
    15721 aaagcagatg acaatgttga agttgaaaag ccaaataacg tggttgaggc cctagctaat
    15781 ggtgctagtg caggtgttac tttggctatt aatgtcggtg ctatgttaat agcatttatt
    15841 gcagtgatcg ctttattaaa tggctttatt ggtggcattg gtaattggtt tggttatgag
    15901 gggctaacct tacaaatttt attaggttat ttattccaac cacttgcttt cttaatcggt
    15961 gtgccgtggc aagatgccga tattgcaggt caaatgatag gtatgaaact tgctgttaat
    16021 gaatttgtcg gatatcttga gtttgctaaa tatctacagc ctgatgctgt ggtgcaatta
    16081 agcgataaaa ctaaggcaat aattactttt gctttatgtg gttttgcaaa ctttagagca
    16141 attgcggtat tgattagtgg tatcggaagt atggcaccaa gtcggcgttc agatattgct
    16201 cgtttaggtt taaaagcagt gattggcggc acattggcga acttaatgag tgcaactata
    16261 gcgggtttct ttattggttt aagtggtgcc acattaggtt gatacaagcg gttaaattga
    16321 ctcacttttt tgcaaaaagc ccaagtaagt taaatgacta cttgggcttt gtttaatgaa
    16381 ctagctctta gtttcggttt ttgctgtgtt atccttttcc gctttggctg tctgtgtttt
    16441 cgaagttgtc tgtttttttg cggattggct agcagtagct gcttttctta ctttctttgt
    16501 tttaacttct acttccgcat ttttagagtt tttatccgct aaccatgcct gcactttagc
    16561 taagaatatt tgtcccatcg ggcgaggatc gccagaaaat aatgtttgta atttattatt
    16621 ttcaataatg tcgcgtctaa ttgctaagcg ttcttcgtgg ttttccgcta aacgaatagc
    16681 ttgagtaacg tattcctctg ctgtttgggt aattaaccaa ttaggtagcc cgaggcgctt
    16741 aaataaacct tcatcaatat gttcatgaac ttcagggcct gttttacaga caccaactaa
    16801 accaagcgta accatatcga taatgccgtt tgtatttcca aatgggaatg gatttagcat
    16861 catatcgcaa ttatgtagaa tatttagata ttcatcatat ggcgaatgtg ggtgtgcggt
    16921 tgcatcattt cctaaatagc ttttgataaa acgttccaca taaggatgcg taattccact
    16981 agattgccct aatgcaaaat ggaaatgagt ttttaccttc gccctatcac gaatggcttt
    17041 taatgcctct aagaaatatg ggtttaattt catagtggtt gatgcaattc cgatatggat
    17101 aacctctgga ttttcacgta ggttatagac cacattttga ggcgctaatg cggaaggcac
    17161 atagggtagc gcatcttttg gaagacgtaa taattgttca ctaaagcagc tttcggaacc
    17221 aacatagtca tcttctacga tgacatattc aataaaatca gagtgtgtag ttgccggatg
    17281 acctagcgca acagcttgaa ttggtgctag gcgagtgtta ctagcgaaaa tagtggttaa
    17341 atccattcca atgctaggca tatagaatac ggctggatta tttttatcac agatatcttt
    17401 taaaaagctt aagcggtcaa atagtgagcc atcttgtggt aataaatgaa attcgtcgaa
    17461 tacctgttgc cccatttcat ctacagcatc actgcctagc ccaataagat ggaaatgttc
    17521 gcgtgcagca accattgagg ttgaatgagt acgataaata gaatgtgatg aatggaaatg
    17581 ctccaataat actaccatga caggtttatt atttctggta ccaagctgtt caatttttct
    17641 atcttcccaa ccgacagaaa gtaaatgacg acgaattaca gcatttaacg accgttttac
    17701 attatgttta tttgcttcaa tatcataact gcaatgcata taaacatcgt gtgaaatggc
    17761 gctcggtagc ttgttcaagt tttcaatttg cgctaatttt tctgggaacc attgcaaaat
    17821 agccgcgcgt tttgcaaaag cagaggatgt accaataaat ctaggtgatt ggagtgcaaa
    17881 acaaagtgat gcggtgagat ctgatgaagc attccatgca gcatctagat tcaataaaat
    17941 attagattcc ggtaagtata aaatacagaa cttaattagt gcttgtaatg tggcatctaa
    18001 atcaaccgta tttggattag cagattcttt atttctattg taagtacgca agatatggtc
    18061 tgcgtttacg tatggagaac tggcaaaaat taatgctatc cagcgttgta atgtcagaaa
    18121 acgcatagca ccgtgttcgg agatctctaa tgcaggatct tcaaacaagc gagtaatcgc
    18181 taccgccatt cgagtacaga aatagatagc tttatcattt tctaaatttt caatctgctc
    18241 tggcatattc aattcaatat tggaaatgcc accaaaatta ctatctaatt ttgaaaggat
    18301 agacagtagc tcggtacaag ccgattcata atcttttttc gcgaccgcct gctcaaaacg
    18361 gatgacgcta ggcatatttt ctgctgacat aatgtatcct tagttgttta aaattcccca
    18421 cagatcatac tcatctgcat gtgtaatggt tactgaaata atttgcccta ctgtcactaa
    18481 ttggtttgaa ggattatcta catagactac accgtcaatt tccggtgcat ctgccattga
    18541 gcgaccgata atgccttctt cgtcaatgtc atcaataatt acttgtacgg ttttgccgat
    18601 ttttgcttgt aagcgagcag cggaaatttc ttgttgtagt tgcataaaac ggtggaaacg
    18661 ttcctctttt acttcttccg gaacttgatc cggcatatcg gtagcaacag caccttccac
    18721 tgggctgaat ttaaagcaac caacacgatc taattgtgct tcttttaaga aatcgagtaa
    18781 tagttggaaa tcttcttctg tttcccctgg gaagccgaca ataaaggtag aacgcagagt
    18841 taattcaggg caaatttcac gccatttttt aatgcgttct aaggttcttt cgattgaacc
    18901 cgggcgtttc atcgctttta agattttcgg gctggcgtgt tggagtggaa tatccaaata
    18961 cggcaaaatt ttgccggcag ccataagcgg aattaaatca tctacgtgtg ggtatgggta
    19021 aacatagtgc aaacgcaccc acacgcccat tgagcctaat tgctcacata gcgtaattaa
    19081 attactttta atcggcatac cgttccagaa aacggttttg gttgcttctt cttttttgcg
    19141 atctaacgaa taggctgagg tgtcttgtga gacgattaac agctctttta ccccggcatc
    19201 aactaggcgt tttgcttcat ctaatacttg cacgatagaa cggcttgcta aatcgccacg
    19261 cattgaagga ataatgcaga aagtgcagcg atgatcgcag ccttcggaaa ttttcaaata
    19321 agcgtaatgc ttcggtgtta atttaatccc ttgttttggc actaagctgg tgtaagggtt
    19381 aaattccggt tttggcacat atttatggac gtggttcatt accgcctcat agctatgtgg
    19441 ccctgtaatt tccaatactt tagggtgtac ctcacgaatt tggttttctt ttgcccctaa
    19501 acagccggtg acaattactt ttccgttagc ttctaacgct tcaccaatgc tttcaagaga
    19561 ttcttgtact gcggaatcaa taaaaccgca ggtgtttaca atcacaagat ccgcattttc
    19621 ataactaggg atgatgttat aaccatccgc acgcagttcg gtcaaaatac gttcggaatc
    19681 aactaaattt ttagggcagc ctaagctgat aaaaccaata tttggtgaag cactcatata
    19741 actctcaata actaaaaaga aaaatgccta caatttttgt ctgtaggcaa aaaactaaca
    19801 agattctaca aaatcttgcc caaaatagcg ataacaagcg gtcaattttt aataaacatt
    19861 tacaaacttg aaatttcgct aacaattaaa attaatcgat attatttagc attgctaata
    19921 caatcgtgga agatttttct gcggctaatg gcagaaattc ttcaaaagaa aggtgggatt
    19981 ctttgtccgc tacatccgat actgctctta cgattacaaa tggaaggtta aaaccgtgac
    20041 aaacctgcgc aatggctgct gcttccattt cgactgcaac gacattcggg aaattctgac
    20101 gaatttgatt aatcttatca ttaccgttaa taaaggcatc gccgctacag attaagcctg
    20161 aaaccacatt gtaaccggct tttttcgctt ccttttgagc taattcgact aattgctcgt
    20221 ttggcgaaaa tgttggcggg tttgccggta gttgcccgat ttcataacca aaggcggtca
    20281 catcaacatc gtgataacga acttcggttg aaatcaaaat atcacctacg tttaaatctg
    20341 gatctaatcc accggcagag cctgtattaa taatcatatc cggctgggtg agctgtatca
    20401 gtaaggtagt gccgatggcg gcagcaactt tgccgatacc cgattgtaat aatgccacac
    20461 gggtattgtt aattttgcct tcaaaaattt tacaacctgc taattcagtg actttaggtt
    20521 caaccattac ttggcgtaaa atctcaattt cttgtgccat cgcaccaata ataccaattt
    20581 tcattacttt ccttctctaa tgtattgttt attttttgaa aaaaacgcta ttttaacagc
    20641 attaatagtg aggagggggt gtttcttctg cttgtgaagc aacttggctt agttgagccc
    20701 ctttcaattt ctcagcaata tggcgaatct gagtttgtaa cttatcaatt gcaaattgtt
    20761 gctcaataag tgcttgattt aactcttcaa taatcagctc ttggaaagca actttagtct
    20821 ccaattcggt aatttttgcc gataatgtat aattattatc cattttttac taaaaaa
//
LOCUS       DANLXQ010000026        20097 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.026, whole genome shotgun sequence.
ACCESSION   DANLXQ010000026 DANLXQ010000000
VERSION     DANLXQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20097
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.026"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(18..710)
                     /locus_tag="PXD94_000754"
     CDS             complement(18..710)
                     /locus_tag="PXD94_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL6285642.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDAIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     NYLILGVDDREK"
     gene            complement(728..2002)
                     /gene="nadR"
                     /locus_tag="PXD94_000755"
     CDS             complement(728..2002)
                     /gene="nadR"
                     /locus_tag="PXD94_000755"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL6285643.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKESYEKYLNLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIKIESPSYL
                     DRYNQAKEVVEAILNEEPIPLQFKQYNIHEFE"
     gene            2226..3188
                     /gene="ribF"
                     /locus_tag="PXD94_000756"
     CDS             2226..3188
                     /gene="ribF"
                     /locus_tag="PXD94_000756"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL6285644.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQGVFAVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
     gene            3291..6107
                     /gene="ileS"
                     /locus_tag="PXD94_000757"
     CDS             3291..6107
                     /gene="ileS"
                     /locus_tag="PXD94_000757"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL6285645.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNSADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            6232..7779
                     /locus_tag="PXD94_000758"
     CDS             6232..7779
                     /locus_tag="PXD94_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL6285646.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVEVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDTDQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            7849..9216
                     /gene="pssA"
                     /locus_tag="PXD94_000759"
     CDS             7849..9216
                     /gene="pssA"
                     /locus_tag="PXD94_000759"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL6285647.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            9271..10014
                     /gene="rlmB"
                     /locus_tag="PXD94_000760"
     CDS             9271..10014
                     /gene="rlmB"
                     /locus_tag="PXD94_000760"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL6285648.1"
                     /translation="MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(10101..11513)
                     /locus_tag="PXD94_000761"
     CDS             complement(10101..11513)
                     /locus_tag="PXD94_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6285649.1"
                     /translation="MKLLDIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPISKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(11602..12384)
                     /locus_tag="PXD94_000762"
     CDS             complement(11602..12384)
                     /locus_tag="PXD94_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6285650.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            complement(12394..13326)
                     /locus_tag="PXD94_000763"
     CDS             complement(12394..13326)
                     /locus_tag="PXD94_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6285651.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGCWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            complement(13546..15120)
                     /locus_tag="PXD94_000764"
     CDS             complement(13546..15120)
                     /locus_tag="PXD94_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6285652.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKFHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPPFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNDLPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            complement(15379..16959)
                     /gene="prfC"
                     /locus_tag="PXD94_000765"
     CDS             complement(15379..16959)
                     /gene="prfC"
                     /locus_tag="PXD94_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL6285653.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHTEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            17181..18056
                     /locus_tag="PXD94_000766"
     CDS             17181..18056
                     /locus_tag="PXD94_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL6285654.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            18129..18716
                     /gene="pth"
                     /locus_tag="PXD94_000767"
     CDS             18129..18716
                     /gene="pth"
                     /locus_tag="PXD94_000767"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL6285655.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
     gene            18902..19993
                     /gene="ychF"
                     /locus_tag="PXD94_000768"
     CDS             18902..19993
                     /gene="ychF"
                     /locus_tag="PXD94_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL6285656.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDKDELHAIKGYNFLTLKPTMYIANVNEDGFENNPY
                     LDRVREIAEKEGAVVVPVCAAIESEIAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRL
                     LNLQTYFTAGVKEVRAWTVSVGATAPKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEA
                     GAKEAGKWRLEGKDYIVQDGDVMHFRFNV"
BASE COUNT         5786 a         4079 c         4573 g         5659 t
ORIGIN      
        1 ttcagtcaaa tttttggcta tttctctcga tcatcaacac ctaaaattag ataatttcca
       61 ttaatatccg ctaggctacg aaagccgata ttataaatag cacattctct gctgacattt
      121 agattgccat aattaaaatt atcatggtag tggccatgga aaattttgct cgcccctaat
      181 tttcttgcta gctggttgat tacagagaag ccttgagggt gtggctttgg tgcttcgtgt
      241 gtaattaaaa tatcggcttt ttcattttcc aatgcttcaa aatcagaagg gaagatcgaa
      301 gagcggtgcc ttaatggaat accaccacgc caaatttttt ccggagcaga gtattggcaa
      361 aaatggatgg gatcaaaaaa cattggacga ttgggcggca tccaaatatg acctcggaag
      421 acccctccaa tacctgcaat ttttattcct tgaatgtcaa ctactttacc gtgaatattg
      481 cgttctttcc agctactatt ccaaatagca tcaaaggcag caacggtttt actgtcatgg
      541 ttaccatgaa taaaccaaat atcacaatac tgcgaaagcc gatctaattc atcaggagta
      601 gtcaattgaa gatcgcctag aataactaaa gctacctcct ccgtctgctg ttgtagaatc
      661 ggataaagat gctcgtagct accgtgtgga tcgcctgcaa aaagtatcat gttatttcct
      721 gtggcagtta ttcaaattcg tgaatattat attgcttaaa ctgtaatggg attggttctt
      781 catttaaaat agcttctacc acctctttgg cttgattgta gcgatccaaa taacttggag
      841 attcaatttt aatataagga atatggtgtt tttctagtag ttttttcagt aggtgttgaa
      901 actgttgacg ctgttttttc gatcctaaac tgcgtaaacc atcggctacc cattttgtat
      961 tgttagaaag taaaatcgtc acatcaaaag gatattcgcg gatcattgaa tccagaaacg
     1021 ggtgagcctt cccttcatac tgaatacaga acgcttgagt tgtgatgtaa tcggtatcaa
     1081 taatagccac tttatgagca tgtttcatgg cataatcaac gtagcgttga tgtccaagca
     1141 ccatttgcgg atagtcggaa tattgcattg cttgttcatt gccaccaagt tgctcaaaca
     1201 caaactcacg cccatattcc caagcagagg tagtgttaaa tacattcgct aatttgctca
     1261 ccaacactga tttaccactg ctttcaccac ctaaaatcgc aatagtttta acaaagaaag
     1321 gacgaacttc tttcggaata aagcgccagt attgaaaagg attattacga atttgtgttg
     1381 ctgaaatatt aaaggattca cgagtaggat cgacaagttg aacttctaga ttcaaatatt
     1441 tttcataaga ttctttatct tgaatttcac tactaaaaac aatagtagga ttaatgcttt
     1501 tttcttcaaa tagcgcttta acacgaccag accaatcttc ccagccattt ggatagctcg
     1561 gaataccgtc ttcaactaaa tggtgaataa agatctgttt ttgttggtat ttaaaaattt
     1621 gctgtaccca gcgtagccga tcttcatttg tcggcattcg tttcatttta ctttcctgga
     1681 aaagacgtaa atcccgttca gaatcggtac aaacaataac atgtaaaaca tctactttac
     1741 taaatgcttc atagatcata ttaatatgac ctgtgtgaat aggatagaat ttgccaaaaa
     1801 taaccccgga acgctggttg ctatcttcta cgatatctaa aacggaatgc aaggctttaa
     1861 gcttattggc gcttggattt ttgatttttc cgcttactaa ctgattaaaa taagctcggg
     1921 ttaccccagc ttgttcacaa atatcattca ctttgagttt aagctgtttg cgcttttgtt
     1981 gaagataagc aaaatctgtc ataatgattc cctcgatttt ttgatgatta tcatatagta
     2041 aaatgtagca tttgaatata tttttattgt ataaatagca gttttatagc atatctttgc
     2101 tttaataatt agctacacga ccactttcag cactaaaaaa atataacaga ttgaattttt
     2161 ttcctttttt gtagatttct cgttataatc acagcaattt tcacttataa aatggtttat
     2221 cttttatgca acttattcga ggtttccaca acttagacaa acacatcgct ttaaatcaag
     2281 gctgtgttct ctctatcggg aatttcgatg gtgtgcatct tgggcatcaa aatattttgg
     2341 ctcgtctttg tgatagagct ttagatttag gattgccctc agttgtgatg ttatttgagc
     2401 cacaacctcg agagtttttt gcaaaaaaaa gtggaaattc aaccgcttgt aacccgcccg
     2461 cccgcttaat gcgcctgcga gataagctaa aatatttgga agaagccggt gtggattttg
     2521 tgctatgcgt gcgttttagt gaaaaatttg ctcaaatttc ggcagacgat ttcatcagcg
     2581 atttgctcgc taaaagactg aaagtacgct atttaagtgt aggcgatgat tttcgctttg
     2641 gggcaaaacg ggcgggcagt tttgaaactt tacgcaatgc cggcttaaaa tatcgctttg
     2701 cggtggaaga aagccatacc catagcttaa acgaagaaag aattagcagc tcattagtgc
     2761 gagaagcctt acaaaacaat gagttagatt tggcagagaa attactcggc aagccttatt
     2821 cgattctggg acgcgttgca cacggtaata aactcggcag aaccattggt tttcctaccg
     2881 caaatattat gctcaatcgt ttggttacgc cgattcaagg ggtatttgcg gtaaaagtga
     2941 agaccaaaca gggcagtttt aacggaatcg ccaatgtcgg caaccgcccg accattaacg
     3001 gtactaagcc gttattagaa gtgcatattt ttgatttcaa tgcctctatt tacggtgagg
     3061 cgattgaagt gatcttttta aaaaaattac gcaatgaaat aaaatttccc gactttgaag
     3121 cactaaaagc ccagattgag aaagatcgct tgcaagcggt tgaatttttt aaggaatttg
     3181 caaggtgagc ctttctagtt tccagcccca gaggggctga gctatgctta acctagtacg
     3241 gctatgccgt gctaggcata aaacagataa ttttaacgag aaataaaaaa atgactattg
     3301 actacaaaaa caccttaaac ttgcctgaaa caggctttcc tatgcgtggg gatctcgcga
     3361 aacgtgaacc cgatatgtta aaaaattggt atgacaaaaa tttataccaa aaaattcgta
     3421 ctgcctcgaa aggcaagaaa acctttattt tgcatgatgg tcctccgtat gctaacggta
     3481 atattcactt aggtcacgca gtgaataaaa ttttaaaaga tatgattttg aaatcgaaaa
     3541 ccgcattagg tttcgatacc ccttatgtgc cgggctggga ctgtcacggt ttgccgattg
     3601 agctgaaagt agaagggatt gttggtaaac caaatgagaa aatttcggct gccgagtttc
     3661 gccaagcgtg ccgtgactat gcgaaagaac aagtagaagg tcagaaagcc gattttatcc
     3721 gtatgggtat tttaggtgat tgggataacc catacctaac aatgaatttt aacactgagg
     3781 cgaatattat tcgcacgctc ggcaaagtga ttgcgaacgg gcatttatac aaaggttcaa
     3841 aaccggtaca ttggtgctta gattgtggat cttcgttagc ggaagctgaa gtggaatatg
     3901 aagataaagt ttctccctct atttatgtgc gttttaatgc tgtggacgta aatgcggtag
     3961 aagaaaaatt caacgcacag ggtaaaggta gaggggcatt atctgcggtt atttggacaa
     4021 ctaccccttg gactattccg tctaaccgtg cgattgcgat caaccctgaa ttagactatg
     4081 ccttagtcca attaggtgat gagcgtgtgt tactagcggt cgatttagtc gaagatgttg
     4141 caaaagcagc aggcgttgaa tctgctgaga ttttagcaac caccaaaggc gaaaacttag
     4201 aattattacg ctttaaccac ccattctatg attacagcgt gccatttatt tttggcgacc
     4261 acgttaccac agacggtggt acagggttag tgcatactgc accggatcac ggtgcggacg
     4321 acttcgtggt cgctcgcaaa tataacattg aaatggcagg gctaatttcc aatgacggta
     4381 aattcaaagc agacaccccg ttctttgccg gcttaggtgt gtttgaatcc aacgataaag
     4441 tagtcgaaaa attacaagaa gtgggagcat tattaaaatt atcccgcatc aagcatagtt
     4501 atccgcactg ttggagacac aaaaccccga ttatcttccg tgctacccca caatggttta
     4561 tcggaatgga aacccaaggc ttacgccagc aagcactagg agaaatcaaa tcggtgcgtt
     4621 ggatcccaag ctggggtgaa gctcgtattg atacgatggt ggcaaaccgc ccagactggt
     4681 gtatttcacg ccaacgtact tggggcgtgc caatggcgat gtttgtgcat aatgaaaccg
     4741 aagaattaca tcctcgcact ttagaattaa ttgaagaagt ggcgaaacgt gtagaagaaa
     4801 aaggcattca agcgtggtgg gatttagatc ctgcggaatt gctcggtgaa gaagccaaag
     4861 attaccgcaa agtgccggat accttagacg tgtggtttga ttcaggatcg acctatgcct
     4921 ctgttgtaga acaacgccct gaatttaacg gcaattcagc ggatatgtat ttagaaggtt
     4981 cagaccaaca tcgtggttgg tttatgtcat ctttaatgct ttccactgct actaacggca
     5041 aagcaccata caaacaagtg ctaactcacg gttttaccgt agatgagaaa ggcagaaaaa
     5101 tgtccaaatc gcttggcaac gtaattgtgc caagtgaagt gtggaacaaa aacggtgcag
     5161 atattttacg cttatgggtt gcctccaccg actataccgg tgagattacc gtttcgcaca
     5221 atattttaaa cagtgccggg gaatcatacc gccgtattcg taacacagcc cgtttcttat
     5281 tggcgaactt gaacggtttc gatccaaaaa gtgatttagt tcaaccgcaa gagatgatcg
     5341 ccttagaccg ctgggcggtg gcttgtgctt tagaggctca aaacgacatc aaagaagcct
     5401 atgataacta ccaattccac acggtagtac agcgtttaat gcgtttctgc tcgattgaaa
     5461 tggggtcttt ctacttagat attatcaaag accgccaata caccaccaaa gccgacagcc
     5521 ttgcccgccg tagctgccaa acagcgttat ggcatattgc agaagcgtta gtacgttgga
     5581 ttgcaccgat tttatccttc acagccgatg aaatttgggg ctacttaccg caggtggaag
     5641 gcagaagtga atttgtcttt accgaagaat tctacaccgg attattcggt ttaaccgagc
     5701 aagataaatt agatgatgct tactggcaac aaatcttaaa agtgcgtgcg gaagtcaacc
     5761 gtgtgctgga acaagcccgt aaagacaaag taattggtgc tggcttagaa gccaaggtga
     5821 cggtattcgc caatgatgaa attcgcccgt tattagaaca acttggcaat gaattacgct
     5881 ttgtgctgat tacctcacaa gcgattgtta aaccactggc ggaagcagat attgcggaag
     5941 gcgaattagc cggactcgca gtaaaagtgg aaaatgcaga tggtgagaaa tgccctcgtt
     6001 gctggcatta tgctaccgac atcggctcgc actctggcca cgaagaagtt tgcggacgtt
     6061 gtgtggaaaa cgtagccggc gaaggcgaaa aacgcttgtt tgcttaaaat gtaattcaat
     6121 tcatctttct aaggcagagg aaactctgcc ttagcttttc cagcgataga tagacactca
     6181 gtttaaaatt tgcaaaaatt ttcggaaaaa taaccgcttg tattgatgta aatgatgaaa
     6241 ataatcgctt ttctttttat ttttctctcc gttttttctc acactgcttt ggctaattct
     6301 atggctgaac aatgtgtcgc gttaaaatct aactcgccgg aaaaaacggc tatttataaa
     6361 gctgaatggg ttgagaaatc tgctgagttg ccggcttact gtttagtaca aggtgaaatt
     6421 ggaaaacggg tggaggtgga taatgttcct tacggtattc aatttgagct acgtttaccc
     6481 gaacaatgga atgaaaaatt cttatttcag ggggcgggcg gtgtaggtgg ggtgattttc
     6541 cctgccattg gtaagctcta tccgcacggg gcgagtggtg caaatggctt aaacagaggc
     6601 tatgcagtag tgagtaacga cagtggtcac gccactcgag atttagtgtt taccgcagat
     6661 cccgaagctc gctcaaatta tgcctatgcc tctatcggca aagtgaccac ggttgccaag
     6721 cagttgattg ccgaatttta ccgaaaaccg agcaagcaca attacattat gggctgctcg
     6781 aatggtggtc gtgaggcgat gatggcggca atgcgttatc cacaggaatt tgacggtgtg
     6841 attgcaggca gccccggctt tcgggtttcc cgttcagtct tggcggaagt atgggataat
     6901 cgagccttgt tggcagttgc tccgaagaat ggtgatggcg acaaaatttt atcccaagcc
     6961 ctcactcaac aagatttaga tgtgattgcc aatggcgtat taacacgttg cgataagtta
     7021 gacggcttag cagacggttt aattaatgcg tgggaacagt gtgatttcca gccggaaatg
     7081 gtggcaaagc aactggggca gaaaaaagtc gctttaatca aaacgatttt cgagggggcg
     7141 aaaaacagtc gaggcgagct gatttatagc ccttgggcgt atgattccgg cattaacgct
     7201 aaaggctggc gggattggaa attaggtgat agccaaaccg ctcagcccaa cagcattagt
     7261 tttaaaatgg gattgaaaag tttgacccat tactatttag cccccagagt gccgagccgt
     7321 gatccactca gcgtagattt ggatcaagcc agcgaacaag tgaaagcggt agggagcatt
     7381 cacgatacag atcaagttga tttatccgct tttaagcaga acggtggcaa aatgctgatt
     7441 tatcaaggcg tttcagaccc gattttttcg gcagttgatc tcaaaaattg gtatcaaagc
     7501 ctacaacaag cggtcgaaaa tccgcaacat tttgcaaaat tgtttttcgt gtcggcaatg
     7561 aaccattgtg ggcgaggggc aacggtgaat gattttgata tgctcactgc ccttgaaaat
     7621 tgggttgaga aaggactgga gccggaaagt attgccgcca aagccggtga gctttaccct
     7681 aataaagcca agcagatccc gctttgtgct tatccgaaag tcgcaattta tcaaaccgga
     7741 gaagatccta atcgtttgac cagctatcaa tgccgttaag cgaaaatcgc tttattggcg
     7801 atttcagtgt aaaatagcag gcaacgattg aaaattttgg aatcaatgat gttaattttg
     7861 aacaaacaac gtaaagctga atacctattg aaaagcctgc catacttacc acaagtggca
     7921 gagcaggttc agtttctcgc ctcgagcaaa gcgtttaaac aacaaatttt acagctgatt
     7981 caaacggcaa aaaagcgtat ttatataacc gctctttact ttgaaaaaga tgaagccggg
     8041 caggaagtat tagctgcttt atatcaagcg aagcgaaata atcctgaact ggaaattaac
     8101 attttggttg attggcaccg agcccaacgt gggcgtattg gtgaggcaga gaatttatct
     8161 aacgccgact ggtatgcaaa agttaaatac gaacaaggtt taccggcagg gcaagagatt
     8221 gccttttacg gtgtgtcgat taacggacga gaagtgtttg gtgtattgca tttgaaaggc
     8281 ttcgtatttg acgatacgat tttgtatagc ggagcaagca ttaataatgt gtatttgcat
     8341 caatttgaac gttaccgtta tgaccgttac cacgtcattg aaaataaagt tcttgccgat
     8401 agctgggtga cgtttattca acagcatctt ttaaccaatg aagcggtaaa acgtttagat
     8461 acggaaaatc gcccgaaaac ggctgaaatt cgtgcgcaaa ttaagcattt ccgcaaacaa
     8521 ttaagtcggg aagcgtattt atttaacggt gttgctgaaa atggtcgctt agcggtttct
     8581 cctttagtag gcttagggcg acgtaagaat cagttaaata aagtgattga agcgttgttt
     8641 tatcaaactg agcagaaatt aacgatttgc actccgtatt ttaattttcc gcattctctg
     8701 agaacccgta ttgaatggct attggcaaac ggcaaatcgg ttgaaattat tgtgggggat
     8761 aaaaccgcta atgatttcta caccaagccg gacgaaaaat tcacgatggc atcggctctg
     8821 ccttatttgt atgaaaagaa cttacgagcc tttgcaaaac ggttggacgg ttatgtacaa
     8881 aataaacagt taaccattcg cttatggaaa gatggcgaaa acagctacca cttaaaaggg
     8941 gtttgggtgg ataaccgcta tattttgctg accggcaata accttaaccc aagagcgtgg
     9001 cggttagatg ccgaaaatgc aattttaatt tccgatcctc aacatcaatt aagtgaaaaa
     9061 gcagaaacgg agttaaacca aatttgccaa catacgcaga ttttaactca ctacagtgat
     9121 ttggaagttt taaccgatta ccctgaaaat gtgcgtaaat tgctgaaaaa attcggcagg
     9181 gttaagttgg ataaaattgt gaagatgttg ctttagttag gtatttatat gggcgagctg
     9241 atgggttcgc ccaattatta aggataaaat atgagcgaac aaatttatgg cattcacgca
     9301 gtgaaagcct ttttggataa tgcacctgag cgtttaattg aagtgctggt gctaaaaggg
     9361 cgtgaagata aacgcttaat gccactatta aatgaattac aacgtttagg ggtggcaatt
     9421 caacaagtga accgccaaac cttagataac aaatcgcaag gtgaagtgca tcaagggatt
     9481 attgccagag tcgtgccaca aaaagagctg aatgaacacg atttagaggc gatcttaagc
     9541 caaaagaaaa atccactctt gttgatttta gatggcgtga ccgaccctca caacctaggg
     9601 gcttgtttgc gtaccgctga tgcggcaggg gttgatgcgg ttattgtgcc gaaagataaa
     9661 tctgctcagc ttacaccaac cgctcgcaaa gtcgcctgtg gggcagcgga agtgatgcca
     9721 ctgattcgag ttaccaatct ggctcgcaca atgcgagatt tacaagaacg gcataatgtc
     9781 tggattgtcg gcacggcagg tgaggcggaa tcgggcattt atgaagcaaa attaaccggc
     9841 tcgattgcgt tagttatggg ggcagaaggc gacggaatgc gtcgccttac ccgtgagcat
     9901 tgcgaccagc ttatcagcat tccaatggcg ggctcagtat cttctttgaa tgtctcagtt
     9961 gcaaccgggg tctgtctgtt tgaaattgta cggcagaagc tggctgggca ataaggaata
    10021 agtaagaaaa atgcgaagaa ctgatgtctt cgcatttttg tttttagagc cgcaaaaaat
    10081 ctcagaaatt gaaccgcttg ttagcccaca tactcttcca aattaatcac tttatcgcac
    10141 gatttctcaa ttagataagg atcgtgactg accaaaatca gactgcaatt tctcgctcgg
    10201 cactggttga tcagcaattc tatcgtttct tgttgtgtga ttgggtcaag ccgagaagtg
    10261 acttcatcgg caaacagtag taccggttct aagagcaagg ctcgcaaaat tgctactcgt
    10321 tgtagctcgc cgccggagac attttccgca ttgcgttgca aaatttctgg gttgagcgaa
    10381 agttgttgca gtaaacttgg aatcggcagt cgatctaatt ggtgcttgtt aatcacatca
    10441 tcaagcaagg tttttagact gacattcgga gcaaaggctt ccggtgggtc ttggtaaagc
    10501 ttcaacactt ggtgtttttt atggctgtgg ctgtgccaca caacctcgcc cgatttcggc
    10561 ttaagcaaac cacaaagtac atcggcaagc gagcttttgc caatcccact atgcccgaca
    10621 atcccaagca cttcgccttt gtgtaagtca aaggaaaggt tgctaaacag cgttttctta
    10681 ccacgggcaa tactcaaatt tttaaccgaa acaagcggtt gtttttggct taaattttgc
    10741 agatcttgca atggttgcca atgttttgga tcggcactga taagtgcttt ggtgtaaggg
    10801 tgttgtgggt tatttaacaa cgcttttgcc tcaccttgct ccaacagttc gccttttttc
    10861 atcactaaaa tagtgccacc gagttgctcg gcaacttcaa tatcgtgagt aatggtgagc
    10921 aagcctccac cgttttggta ggcggatttt aacagttcaa tcactttgcc tttactgatt
    10981 gggtcaagcc ctttggtcgg ctcatcggca agtaaaattt tgccaccggc ggcagttgca
    11041 atcgcaaaag aggcacgttg agccattcca ccggaaagct ggtgtgggta gttttcaacc
    11101 gattcgccca gcccaagttt ggctaaatag gctttgcttt ccgttttggc ttgagatgat
    11161 ggtttgcctg ccacatagtg aaaactttcc cacaactgct tgccgattgc cataatcgga
    11221 ttaagcgagc ggcgaggctc ttgtggcagc attaccaagg ttttacccca caatgtttgc
    11281 aagcggtcgg attcggtgga aattttgcaa ttttttaccg aaatttcacc gcttgcgatt
    11341 aagccgtcgg gtaacgcccc cataatcgct tgaatcagca ggcttttgcc tgagccggtt
    11401 tcgcctaaga tagtaatatt ttttcctgcc tttaattgta ggctaatcgg ttcgaccagc
    11461 tgtaatctgt tttcggtttc aacagatagg ttttggatat cgagtaattt catcttcatt
    11521 ttattactgt aggggcgggc cctgtgcccg cccataaata taataagaat tttgggcagg
    11581 cacaggacct gcccctacgc attatttaat ttttctagac aataattgta agctgagtac
    11641 cattaagaaa acggcaacca ctggttgcaa aaatatcagc ggtgcttcgt agtagtaagg
    11701 gaatagttcg gtcatcatca agcctaattc tgctgtaggc gggcgaagcc ctacgctgac
    11761 aaagccaagt gtggcgagag ctaaaatcgc attgccgagc gagaatgccg ataaagtggc
    11821 aatcatcggt aataaacggg gcaataagtg gcgtttgaag ctataaaacc agcccattcc
    11881 cattaggcgt gaggcttgaa tttccgagct gtttgccatt gttgaactga tagagcggat
    11941 cacacggaaa aattccaccc acattaccaa tgaaatccct aaatagagcg tccaaaatga
    12001 gccgggagaa agggcggcaa agagcaatac taaaagtaag ccggggagtg ccatcactag
    12061 atcacaaata aagctgaaaa tgcggtcaat ccagccaccg aaaaagcctg ccataatgcc
    12121 ggtaattaag cctgcaatta aggcacaaat cacgctaaac agtgataagc caaacgaaag
    12181 acgaatagca gaggcaagac gggcaagcat atcccgcccg agatgatcgg tgccgaacca
    12241 agcgatttca tttggattgg aaaggatatt ggttaaatct tgaaagccaa tatccatcgg
    12301 gtaaaaataa ggctgtaaaa aggcaaatcc caacattgca aggagaatag tgccgcctaa
    12361 atattgtgaa attgaaattt tcatcttgat gtcctatcgc ttaattcgtg ggtcaatcca
    12421 agcacttaat agatcggcaa tggtgtttaa tacaacaaac aatccgccca tcacgagcgt
    12481 tgtaccttga atcatcggca catcacgggc aatgatggcg tgaaccagtg cgtgtccgct
    12541 gccgggccaa gcaaataggc tttcgaccac cacaacgcct tcaattaaat aaactaattg
    12601 tactgcgtgg taggcaatga ccggcacggc aatattcggc aaaccgtgac gtaaaaaggt
    12661 atgccaacgg cttaagcctt ttaattgtga aaattggtaa aattctgagc cttttacctg
    12721 aaccattgca gaacgggtga ctcgtaccga aaccgtactc aagccaatcg caagcgttaa
    12781 ggcaggcaag ataaaatgtt gccaacagcc gtatccgccg gcaggtagcc attttagttg
    12841 agcggaaaac agcgtaatca agccaatcgc aataataaat gccggcacgg agcgaaacag
    12901 ggtactgaat accagtgtca agcggtcaaa aaagccgttt ggcttacggg cggctaacaa
    12961 accgagcgga ggcccgataa ggattgctaa gataagtgcc acaatcgcaa ggcttaaggt
    13021 atgcccgaat tggtgctgaa tttcagacca gaccggatca ccggtcacca atgatttgcc
    13081 taaattaaat tgagccaagt cacttagcca gctgaagtag ctttgccacc aaggttggtc
    13141 taatcccagc tcggcacgca cagcctcagc agcagcacta tctgcttgat cataaccata
    13201 gcggctggca gcaatgcggt atgccatatc acccgaaagt tggcgggtga ggataaatgt
    13261 gagtgtgcca accgaccaaa tcaccagtag aatttggata agacggcgga agagaatttt
    13321 gtgcattgta ttatttcatt tattaaaaag atgattttat gcgttctgat ccccctcttt
    13381 agcaaagagg ggaagggaag atttggcaga agaaataagc ggagaaaata ggtgaaaaat
    13441 gagaaaaaac gataattctg tcactattta ttcacctaag agcgcaaatt actactgaaa
    13501 aatcctacct tcccttttct aaagcgagga gtattttgaa ttaaactact tacttaataa
    13561 ctccaaaaag aagcgtcttt caaatggatc tagcgtcaca ttttttactt ctttatgtgc
    13621 caccacattt tgttggtaat aaaccaccgg aatgattggc aaatcattgt aaataatttt
    13681 gctaaccgtt tgctttaatg ctttcgcttt ttgcgggtca cgctcggttt cgagcgattt
    13741 gactgcattg tctacctcag tattttgcca attcattacc ccccaatcac tgccaccatt
    13801 ggcgaagtcg gaggcaatta cgccatatgg atctaaggtt ttgccataat ttaaggcata
    13861 aagagccatt tctaaactgc cgtcttgatg ccccgccgga atttcgctga aattacccac
    13921 cgaaacattc acttccacgc caatttgttt ccattgagct tgtaagatag tagcaatcac
    13981 cggtaattca ggacgatctg agaaggtgcg catggtaaag ctaaacggtt tgccgtcttt
    14041 ggttaagcga ccttgttcat caaaggtata gccggaagcg gtcagatttt gtttaatttt
    14101 tgcaatatcg accacttgtt ctgcggtatc aacacgccag tcggcaaagg ctttcggtaa
    14161 aatttgatcg gcagctccgt tctcaatttt taagacctgc tcagcaatcg ccttgcgatt
    14221 aatcgcttgc gataaggctt ggcgaatcgc taagtcgtta aaaaacggct ttgccacgtc
    14281 cattttcaac tgaatagtac gggcaataga tccgctgact aattgcaggt taggatcggc
    14341 tttcagcctt gctacactgg cggtatctaa attaaatacc agtgatgacg gctcgctttg
    14401 tgccattaac atacgggttt cgctacggct gctggctaaa taattggctt gctgaacttg
    14461 cggttttttg ccccaatagc cctcaaattg caccgcttcc aatttctgcg gagcttcaat
    14521 ttttgtgggt ttaaacgcac ctgtaccgat aatttgggtc acttcgcctt tttcattata
    14581 gctttcaggt gccaaaatta aggcggtgta gtgtgttaaa aagggaggaa acggcacaaa
    14641 agctttagtt agctcaattt ccactttgtt atctaccgct cgaatttcct tgataaaggc
    14701 ttttgctaac acacccggtt tgctttttgc gatattcaaa ctatttacca ctgcttgtgc
    14761 ggtaagtact tgatcgttat ggaattttac cccttcacgc aaggtaaaac gccagattgt
    14821 tccctcttcg ttactgcgcc agtcagtggc caatgccgga attaaattcc cgttaagatc
    14881 ggcttcgacc aatgtttccg ctaagttcat acgctggaaa attacgcctg attgattcgg
    14941 gtcaaggcta ttgatttccc atggggcaac cacggttgca agcggtaata tttccgcatt
    15001 tttttgcgat tgagtattct ctacttgcgg tttttctgcg gttgcttgag tgctgttctt
    15061 atcatcgcaa gcagaaacac agaacgctgc gacagttaat ggaatcaacc attttttcat
    15121 tttattttct ccataaaaaa atcagggcta tgaatattgg cgaactttag catatttcca
    15181 taaaaaaagt agagttttct gcgagggtat ttttgagtgg ttaattaata ggcagaataa
    15241 tctcaagata aggattgttg ataggggaat atttaggcta tttgaggaac agaatgccta
    15301 gtattgtaaa attcggaaaa aaaattgacc gcttgtgctt tatttgtagg ggcgaaagat
    15361 ttttcgcccc tattggtttt agtgctctct cgttttaaag aactgtacgt cagggtaacg
    15421 ttcttgggcg aggtttaagt tcaccattgt cggagcaatg taggtgaggt tgtctccgcc
    15481 gtctagggca aggtttgcct cgtttttgcg tttgaactct tcaaattttt tggcatcttt
    15541 gccttcgacc caacgggctg tggcaacgtt tacggtttca taaattgcct ctacaccgta
    15601 ttcggttttc agccgtgaaa ccaccacatc aaactgtagc accccaaccg ctcccacgat
    15661 caaatcgttg ttgattaacg gacggaatac ttgcaccgca ccctcttcgg aaagttgtac
    15721 caagcctttg agcagttgtt tctgtttgag cggatcacgc aggcggattc ggcggaacag
    15781 ctcaggggcg aagttcggaa taccggtgaa tttgaaattt tcgccttggg tgaaagtatc
    15841 accaatttgg atcgtgccgt gattgtgcaa gccgataata tcgccggcgt aggcttcttc
    15901 ggcttgtgaa cggtcgcccg ccataaaggt taaggcatcg gagatcacca catctttgcc
    15961 gatacggacg tgtttaagct tcattccttt ttcatattta cctgaaacga cacgcataaa
    16021 agcaactcga tctcggtgtt tcggatccat attcgcttga attttaaata cgaaaccgct
    16081 gaatttttcc tcgcttggtg aaacttcacg ttcgtccgat tgacgggctt gtggtgccgg
    16141 tgcccattcg gttaaaccgt caaggaaatg attgaccccg aagttaccca gtgcagtgcc
    16201 aaagaaaacc ggtgtcagct cgcctttgat aaattcggtg tgatcgaatt cgtgcgaagc
    16261 gccttgtacc agctctaatt catcacgcaa ctgctgggct aaatcatcgc caacggcttg
    16321 atccagttcc ggattattta agcctttaac cactcgtact tcttgaatgg tatgaccttg
    16381 tccggtttgg tataagtagg tttcgtcctt gtaaaggtga tatacccctt taaacaattt
    16441 tccgcaacca atcggccaag tgatcggcgc acatttgatt ttcagcacgc tttccacttc
    16501 atctaacagc tccatcgggt cacgaatatc acggtctagc ttgttcataa aggttaggat
    16561 tggcgtatca cgcaagcggg tcacttccat taatttaatg gtgcgttcct caaccccttt
    16621 tgccgagtcg ataaccatta agcagctatc taccgcagtc aaagtacgat aagtatcttc
    16681 cgagaagtct tcgtgccccg gtgtatcgag taagtttacc aagcaatcat tgtacgggaa
    16741 ctgcatcacg gaggtggtaa tcgaaattcc acgttgtttt tccatctcca tccagtcgga
    16801 tttagcgtgt tgtgccgagc ctttgccttt taccgagccg gcagtttgga ttgcatttcc
    16861 gtagagcaat actttttcgg taatggtggt tttacccgca tcggggtggg aaataatcgc
    16921 aaaggtgcga cgtttattaa cttcttgtgg gtatgacatt tttctcaacc aattaaaata
    16981 aaaagggcgg aaagtccgcc aaaaaattaa gcgttatttt cgctgatttg aggcgagagg
    17041 gcaagcggtt aaatttggcg gaaattttgc aaaatcggct agatttgcaa aaattttgta
    17101 aaaatcgacc gcttgctttg gcacaatttt ggaaacaggg tagaataacc agctttgact
    17161 ttattatcga tgaatctatt atgatgaaaa aatttattcc tgttatgatt gcggctctgg
    17221 tgttaagcgc ctgttcctcc cagccggcaa aaaggaaaag cacaccacgg attaaagcaa
    17281 ccgctgctta tattccaacc gaaaattccg cttatgtggt gtttgactta aaccaaaaga
    17341 aatttttgga aggtaagaat ttcaatttag ttcgcccgat tgcttcggtc acaaaattaa
    17401 tgacagcagt cgttttctta gaagaaaacc gctatggggc gagttgccaa acccaaattt
    17461 tgccgtcaga tgctgatttc ttaaaaggca cgaccagccc attgccgaaa aatcttaaca
    17521 ttgcctgtag tgaattgtta aaagcgatgc tagttcgctc cgataactat gccgctcacg
    17581 cccttgccca cgcaacacat ttgaataaag cccaattttt ggctcgaatg aacagcaaag
    17641 cccgtgaaat cggtatgact caaacctact ttggtgatag ctcaggctta tcttcggata
    17701 atgtttcaac ggtggttgat cttgctaaat tggcaggcta tgcggcaagt aagcctgaaa
    17761 ttcagcaatt gtctaattcg ccattagtga gtgtttatgc aggtggtcgt tatttctcta
    17821 tgaaaaacac caacagtatg gttcgttccc aatcctatca ggcattagtg agtaagacag
    17881 gttatacccg tgaggcgggt tataatcttg cctttattgc taaaggggat tgcagaggca
    17941 agcgaattgg tgtggttagt ttaaacaatg ataacgctgc ttcacgcacc gatttcactg
    18001 aatcgatgtt agcgaagtat caatgcgtac caaatccgta tagacaatat gattaatggt
    18061 tgccttgaga aaatagcggt ttttctctaa aatactgcct tttaaattaa atgaattgag
    18121 acaagattgt gtcagaaatt aaattaattg tcgggctggc gaacccgggg gtaaaatatg
    18181 aagaaacccg ccataatgca ggcgagtggc tgattaacga acttgcccga atatataacg
    18241 ttagcctaaa agaagagccg aaatattttg gcaaagtagc aaaaattaat accgctcaag
    18301 gcgaagtccg tttattagtg ccgactactt ttatgaattt aagtggtaaa gcagtgggag
    18361 cgttagccaa tttttatcgg attaaaccgg aagaaatttt ggtcgctcac gatgaattgg
    18421 atttaccacc cggttcggtg aaattgaaaa aaggcggcgg acacggtggt cataacggct
    18481 tgaaagatat tattgccgcc cttggcaata ataaagagtt ttaccgtgtg cgaattggta
    18541 ttggacaccc ggggcataaa gacttagtgg cgaattatgt gctgggcaag ccttcaccaa
    18601 gcgatcagac attactgaat agtgcggttg atgaagccgc acgttgcgta gaagttttat
    18661 ttaaagacgg aatggttaag gcaactaacc gtttaaatag ttttaaagca aattaaaact
    18721 ctctcccttg tgggagagag accgaaaaat acttcgttca gaagtatttt tcagagagag
    18781 gggagcggtc atatttgagg aaattattgc aatttcgccc tctccctcat tttcgcttct
    18841 gaacgaagct caaatttatc cctctcccgc aaggggagag gggaattcta aggaacaaaa
    18901 aatgggattt aaatgtggta ttgtgggttt accaaatgtc ggtaaatcga cccttttcaa
    18961 tgcattaact aaagcaggta ttgaggcagc aaactatccg ttctgtacga ttgagccaaa
    19021 taccggtgtg gtaccaatgc ctgaccctcg cttagatgcg ttggcggaaa ttgtgaaacc
    19081 tgagcgagtg ttaccaacta caatggaatt tgtggatatt gccggcttag tggcaggtgc
    19141 aagcaaaggc gaaggtttag gtaataaatt cttagccaat attcgtgaaa ctgatgcgat
    19201 tggccacgtt gtgcgttgtt ttgaaaatga tgacatcgtt cacgttgcgg ggaaaattga
    19261 cccagctgat gatattgaaa tcatcaatac cgaattggca cttgccgatt tagacagttg
    19321 cgagcgtgcc attcagcgtt tacaaaaacg tgcgaaaggt ggcgataaag acgcaaaatt
    19381 tgaattatcg attatggaaa aaatcctacc tgtgcttgaa aatgccggta tgattcgttc
    19441 ggtagattta gataaggatg aactgcacgc tattaagggc tacaacttct taactttaaa
    19501 accgacaatg tatattgcca acgtgaacga agacggtttt gaaaataacc cttatcttga
    19561 ccgtgtgcgt gaaattgccg aaaaagaagg ggcggtggtt gtaccggtgt gtgcagcgat
    19621 tgaatcggaa attgccgagc ttgatgacga agaaaaagtg gaatttctac aagatttagg
    19681 cattgaagag ccgggcttaa accgtgttat tcgtgcaggc taccgtttac ttaacttaca
    19741 aacctacttt accgcagggg taaaagaagt gcgtgcgtgg acggtttcgg taggtgcaac
    19801 cgctccaaaa gcagcagccg tgattcacac tgactttgag aaaggcttta ttcgtgcgga
    19861 agtaattgct taccaagact tcatagacaa taaaggtgaa gccggtgcaa aagaagcggg
    19921 taaatggcgt ttggaaggta aagattatat tgttcaagac ggcgatgtaa tgcacttccg
    19981 tttcaatgtt taatttgcaa atttttgcct aaaaaagacc gcttgttgat ctgtgatcta
    20041 caagcggcct ttttttgtta attaattgca tattgctcga ttcggtaaaa atgtctt
//
LOCUS       DANLXQ010000027        20030 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.027, whole genome shotgun sequence.
ACCESSION   DANLXQ010000027 DANLXQ010000000
VERSION     DANLXQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20030
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.027"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            8..604
                     /gene="plsY"
                     /locus_tag="PXD94_000769"
     CDS             8..604
                     /gene="plsY"
                     /locus_tag="PXD94_000769"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL6285657.1"
                     /translation="MPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGATNVL
                     RIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQFRGG
                     KGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEPQFT
                     FPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            complement(678..2585)
                     /gene="parE"
                     /locus_tag="PXD94_000770"
     CDS             complement(678..2585)
                     /gene="parE"
                     /locus_tag="PXD94_000770"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL6285658.1"
                     /translation="MSEKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNS
                     VDEALSGFADKIDVILHADNSLEVVDNGRGMPVDIHSTEKISGVELILTKLHAGGKFS
                     NKNYTFSGGLHGVGISVVNALSERVEIQIKRGGEVYSIAFANGVKVEDLTVIGTCPKK
                     QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTINFIDKINNTEESWYYED
                     GLSDYLSEALNGYETVPNPPFIGDVTAETEAVSWALTWLPEGGELVAESYVNLIPTAQ
                     GGTHVNGLRNGLLKAMVEFCEIHNLLPKGVKLTADDVWNRCAYVLSLKIQEPQFAGQT
                     KERLSSRQASSYVDSTLKDAFSLWLNQNVQTGKLIAEMAISSAQSRMRAAKKVVRKKL
                     VSGPALPGKLADCTAQDLSRTELFLVEGDSAGGSAKQARDKEYQAILPLRGKILNTWE
                     VSSDQVLASQEVHDIAVALGIDPDSDNLEELRYGKVCILADADSDGLHIATLLCALFL
                     RHFPKLVQNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKPNVQRFK
                     GLGEMNPSQLRETTMEPSTRRLVQLTFDDTVEEGELNTFEIMDMLLGKKRAEDRKQWL
                     QNRGDEADLAV"
     gene            2793..3017
                     /locus_tag="PXD94_000771"
     CDS             2793..3017
                     /locus_tag="PXD94_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL6285659.1"
                     /translation="MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEE
                     REGSRIAVVLFQQVRVFHRPHPNPDTDKGR"
     gene            3060..3389
                     /locus_tag="PXD94_000772"
     CDS             3060..3389
                     /locus_tag="PXD94_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL6285660.1"
                     /translation="MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQL
                     KKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSPELHKAAVIAATAENLSLNEWIN
                     QTLEKSV"
     gene            complement(3428..3814)
                     /gene="cueR"
                     /locus_tag="PXD94_000773"
     CDS             complement(3428..3814)
                     /gene="cueR"
                     /locus_tag="PXD94_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL6285661.1"
                     /translation="MNISQAAEKTGLSAKQIRDYEKAGLLPPTSRTEAGYRFYNATDL
                     SRLHFISNARKVGFSLAQISELLKLNDNPNRSSRDVKQLTEQHIGELEQKIADLNQML
                     TLLKSWSSSCCGNANSECSILNGLNG"
     gene            3903..4103
                     /locus_tag="PXD94_000774"
     CDS             3903..4103
                     /locus_tag="PXD94_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL6285662.1"
                     /translation="MTITLQLDGLHCGNCVKSVEKALNGVAGVNQVSVTLEPQIAVVE
                     GSAEAQALIEAVEDIGFDAKLA"
     gene            4156..6348
                     /locus_tag="PXD94_000775"
     CDS             4156..6348
                     /locus_tag="PXD94_000775"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL6285663.1"
                     /translation="MTSTAHYSPEQQLLIDGMHCAACVRRVEKALLKVEKVEFASVNL
                     ADQTAFVQGNANPEALVAAVVKIGFGAEVLESEEERRIKQQAQTQRVLGHKRTQFMLA
                     LVVGFALVAFGFYKGMTVNETNRSLWLSWAGISLATMYFSGKDFFLGAFSALKNRSAN
                     MDMLVAISTGTAWLYSCWLTLFPQPNAHVYFEASVMILGFINLGKYLELKAKQRSSLA
                     LEKLLDLAPKQAVIFEENIAKTIPAKAIKPQMRVQALTGDRLAVDGILDSGSIWVDES
                     MLTGEALPVEKKIGDKVRAGTLVQDGSGTYIAEQVGSQTALARVINAVRHAQSSKPPL
                     AQFVDKIAAVFVPVVVSIALLSALIWLAVGQDFAFALSIFTTVLIIACPCALGLAIPL
                     STIAGVARSAEFGVLVRNIEALQASSEIDTLVFDKTGTLTTGRMEVSDVLTFNGFEQK
                     RLLQLAKSVEQHASHPIAKAIAKFAENQTACGVTDVQVLKGLGISAKYEGSTIRMGNW
                     QFVGETIQANLASLLPAKEGDFSSKIFVSVDNQLAGVLSVSDQLRPEAKATIHQFQAE
                     GYQCVMLTGDRQQTADYFANALGLDGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGIN
                     DAPALAQANVGIAMHNGSDIAVETADLSLMREGLAPLMQILPFSKKVLKNMKQSLLGA
                     FIYNVIAIPIAAGVLYPFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(6401..7186)
                     /gene="truA"
                     /locus_tag="PXD94_000776"
     CDS             complement(6401..7186)
                     /gene="truA"
                     /locus_tag="PXD94_000776"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL6285664.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGRQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPKTPLGPLFLAD"
     gene            complement(7251..7940)
                     /gene="folE"
                     /locus_tag="PXD94_000777"
     CDS             complement(7251..7940)
                     /gene="folE"
                     /locus_tag="PXD94_000777"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL6285665.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVTHTKDKAS
                     RRAEIQQHMQAVLELLGLDLSDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            8041..8748
                     /locus_tag="PXD94_000778"
     CDS             8041..8748
                     /locus_tag="PXD94_000778"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL6285666.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     TTWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPVAIKIERHFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFCLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(8803..9900)
                     /gene="apbC"
                     /locus_tag="PXD94_000779"
     CDS             complement(8803..9900)
                     /gene="apbC"
                     /locus_tag="PXD94_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL6285667.1"
                     /translation="MNQLNEQQLVEIKAILQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGSSGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGTRVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(9897..11444)
                     /locus_tag="PXD94_000780"
     CDS             complement(9897..11444)
                     /locus_tag="PXD94_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6285668.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLTAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSALLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPR
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANSGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSINIANQVTRALSQSELFNITPKMVDWQTL
                     NKVREQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTETL
                     SAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLKRN
                     LANKDNQ"
     gene            complement(11470..12120)
                     /locus_tag="PXD94_000781"
     CDS             complement(11470..12120)
                     /locus_tag="PXD94_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL6285669.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVSIGVILLVSLYFGNLILNAFSISLSSFRVAGGILIVSIAMTMISGKLGEHK
                     QNKEEKNEDITEYENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVF
                     ALICYILFRYSAPLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
     gene            complement(12242..14113)
                     /locus_tag="PXD94_000782"
     CDS             complement(12242..14113)
                     /locus_tag="PXD94_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL6285670.1"
                     /translation="MIEKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKV
                     NGEEISQQLFNNTLNREQNRLNAELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQ
                     YAKELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGML
                     QSQLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYE
                     ANKTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQ
                     VASEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVAN
                     LTAGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYFTVARE
                     MANKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQN
                     SDALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENV
                     KALNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVV
                     IVALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
     gene            14295..14564
                     /gene="rpsO"
                     /locus_tag="PXD94_000783"
     CDS             14295..14564
                     /gene="rpsO"
                     /locus_tag="PXD94_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL6285671.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            14636..15505
                     /locus_tag="PXD94_000784"
     CDS             14636..15505
                     /locus_tag="PXD94_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL6285672.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
     gene            complement(15595..16860)
                     /locus_tag="PXD94_000785"
     CDS             complement(15595..16860)
                     /locus_tag="PXD94_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL6285673.1"
                     /translation="MFRTLFLMLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVML
                     VVFFVLAMAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSK
                     AEKLFSKNAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRT
                     RILLQQGKLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNF
                     LTAEEYAELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDS
                     DDHQSAQEIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDD
                     YARALGYIYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREE
                     NLKQVNMATQSKVNEPLALPQTATESNSL"
     gene            complement(16879..18189)
                     /locus_tag="PXD94_000786"
     CDS             complement(16879..18189)
                     /locus_tag="PXD94_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL6285674.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAGSFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQLNKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPLSAQ"
     gene            complement(18201..18965)
                     /locus_tag="PXD94_000787"
     CDS             complement(18201..18965)
                     /locus_tag="PXD94_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL6285675.1"
                     /translation="MNVLVTRLDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(18965..19900)
                     /gene="hemC"
                     /locus_tag="PXD94_000788"
     CDS             complement(18965..19900)
                     /gene="hemC"
                     /locus_tag="PXD94_000788"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL6285676.1"
                     /translation="MKDILRIATRQSPLALWQANFVKSELEKHFPQLSVELVTMVTKG
                     DIILDTPLAKIGGKGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICERED
                     PRDAFVSNNYHSLEDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDN
                     GEYDAIILASAGLIRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKL
                     NHSSTACCVMAERAMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAK
                     GHKQEAEQLGILVAEQLLAQGADKILAAVYNENVQ"
BASE COUNT         5626 a         4411 c         3791 g         6202 t
ORIGIN      
        1 gaatttaatt ccctatttat tgatcttaac cgcctatttg ctcggctcga tttccagtgc
       61 ggtcattttt tgtaagctgg caggcttgcc tgacccaaga gaagtcggct cacacaatgc
      121 cggtgcaacg aacgtactac gaattggcgg taaaagtgcc gcattaggtg tgcttttatt
      181 cgatatttta aaaggcacat taccggtggc gatggggatt tattttgaac tctctccctc
      241 acaactcggt ttcatcgcct tagcggtttg tcttgggcat attttcccga ttttcttcca
      301 attccgtggc ggaaaaggcg ttgccaccgc atttggtgca atctccgcat taagctttat
      361 tgttgcaggc ggtgctatcg gcacttggct actggtgttt ttaatctccg gctattcttc
      421 tttaagtgcc gtcttcactg ccttggtcgt accgttttac atttggtggt tcgagccaca
      481 atttactttc cccgtcgcct tagtttgttg cctattggtt taccgccatc acgataatat
      541 tcaacgctta tggcgtgggc aggaagacaa actgtgggat aaattcaaga aaaatagagc
      601 ataaaaatag gcatagtgaa ccgttcgcta tgcctatttg caaattattg cggaaaaatc
      661 accgcttgtc ttctcgacta aaccgctaaa tctgcttcat ctcctcgatt ttgtagccat
      721 tgtttacgat cttccgctcg ttttttaccc aatagcatat ccataatttc aaaggtattc
      781 aattcgccct cttctaccgt gtcatcaaag gttaattgta ctaagcggcg agtgcttggc
      841 tccatcgtag tttcacgcaa ctggctcggg ttcatttcgc ctaagccttt aaagcgttgc
      901 acatttggct ttcctttttt cttggcaagg cgagctaaaa tcgcctcttt ttccgcctca
      961 tctaaggcat aatgcacctc atctttgccg atatcaatgc gataaagtgg tggcattgcc
     1021 acataaacgt gtccgttttg caccaatttc gggaaatgac gaaggaataa cgcacacagc
     1081 aaggtggcaa tatgcaaacc gtctgaatcc gcatcggcaa gaatacaaac tttgccataa
     1141 cgcaactctt ctaaattatc gctatcaggg tcaattccca aggctaccgc aatatcgtgt
     1201 acttcttgtg aagccaatac ttggtctgat gaaacctccc aagtgtttaa aatcttacca
     1261 cgcagtggca aaatggcttg atactcttta tctcgagctt gttttgccga accgccggca
     1321 gaatcacctt ctaccaagaa aagttcggta cggctgagat cttgagctgt acaatccgcc
     1381 agtttgcccg gtaaagccgg accactcacc aatttcttac gcaccacttt tttcgccgca
     1441 cgcatacggc tctgggcgga actaattgcc atttcagcaa ttaatttacc tgtctgcaca
     1501 ttttggttaa gccataaact aaaggcatct ttcaaggtgc tatccacata gcttgaggct
     1561 tgacgggaag acaaacgctc tttggtctgc cctgcaaatt gtggctcttg aattttcaga
     1621 gaaagcacat aagcacaacg gttccacaca tcatcagcgg ttaattttac ccctttcggc
     1681 aataaattat ggatttcgca aaactccacc atcgccttta ataaaccgtt acgcaagcca
     1741 ttaacgtgcg tgccgccttg tgcggtcgga atcaggttca catagctttc cgctactaac
     1801 tcgccccctt ccggcaacca agttaatgcc cagcttaccg cctctgtttc agcagtaaca
     1861 tcaccaataa acggtggatt cggcacggtt tcataaccgt ttaatgcctc gctcaaataa
     1921 tcagataagc cgtcttcgta ataccaactt tcttcggtat tgttgatttt atcaataaaa
     1981 ttgatggtga gtttcgggca aagcaccgct ttggctcgta acaaatggcg taaacgactc
     2041 accgaaaaac gagggctatc aaaatatttt gggttcgggt aaaaacgcac tgtcgtgccg
     2101 gtctgttttt tcgggcaagt accaattacc gtcagatctt ccacttttac cccgttggca
     2161 aaggcaatcg aatagacttc tccgccacgt ttaatctgaa tttcaacccg ttcggacagg
     2221 gcatttacca ccgaaatccc caccccgtgc aaaccaccgg aaaacgtata gtttttattg
     2281 gaaaatttac cgccggcgtg caatttcgtt aagataagct ccacccctga aattttctcg
     2341 gtggaatgaa tatcgaccgg cattccccgc ccgttatcca ccacttccaa cgaattatca
     2401 gcatgaagaa tcacatcaat tttatctgca aagccggaca acgcctcatc gacactatta
     2461 tcaatcacct cctgccccaa atggtttgga cgtgtggtat ccgtgtacat tcccgggcga
     2521 agctgcaccg gctcgaggtc tttcagaacg gtaatttcat ccgccccata gcgtttttca
     2581 gacattatgc tttctcttag gcttaaaata aaataaggct gaattttaag gaatattcag
     2641 ctattttgca aaattttggt gaaaaatgac ctgttgttat gcaataaaaa ctatttttgc
     2701 gatctacatc gaaaaaatga gttttaattt ccctatttgc ttattcattc agtatcttaa
     2761 tctcactttt tattttaaaa tggtactata ttatggtagt aaaaatccta aagcggaaat
     2821 ctcaaaagac actacaacaa atctacaccc agcctatttc tgccaatatt aaatggacaa
     2881 atatagaagc tctttttcta gagttaggag cagaaataga agaacgagaa gggagcagaa
     2941 ttgcagttgt tttatttcaa caggtcagag tatttcatcg cccacacccg aatcctgata
     3001 cagataaggg gcggtagcga gtattaaaaa atggttatct gaaaacggag tcattcctta
     3061 tgcgtaatat tatggatatt aatggttata aagctgtcat tgcttacgat cccgaaacag
     3121 aattatttcg aggtgaattt atcggattaa atggtggagc cgatttttat gccgataatg
     3181 tcattcaatt aaaaaaagaa ggtgaactaa gtttgaatat ctttttagaa ctttgtagag
     3241 aaaaaggtat tgagccgttc aaacaatatt ccggaaagtt taatgttcgc ctttcgcctg
     3301 aattacataa agctgcggta attgcagcaa ctgctgaaaa tttaagctta aatgaatgga
     3361 ttaaccaaac gttagaaaag agcgtgtagc ctaattgcaa aacaatcacc tattttgacc
     3421 gcttgtatta cccgtttaac ccattcaata tcgaacactc cgaattggcg ttgccacagc
     3481 aagagctact ccagcttttg agcaaagtca gcatttggtt taaatcggca attttttgtt
     3541 caagctcacc gatatgctgt tcggttaatt gttttacatc tcggcttgag cggtttgggt
     3601 tgtcgtttaa tttaagtagt tcgctgattt gagccagcga aaagcccact tttcgggcat
     3661 tgctgataaa atgtaatcgg ctcaaatcag tagcattgta gaatcgatac cccgcttcgg
     3721 tgcgacttgt cggcggcaga agtcctgctt tttcataatc tcggatttgt ttggcggaaa
     3781 gcccggtttt ttctgcggct tggctaatat tcattataat cctctcactt taatcgcttg
     3841 accttaacct aaggttaagc tttattatac gcctgaataa tcactcaaaa caggagaaaa
     3901 tgatgacaat taccctacaa cttgatggct tacattgtgg caactgtgtg aaaagtgtcg
     3961 aaaaagcgtt aaatggcgtg gctggggtga accaagttag cgtaacgtta gaaccgcaaa
     4021 ttgctgtggt ggaaggctca gcagaagcac aagccttaat tgaagcggtt gaagacattg
     4081 gctttgacgc aaaattagcc taattaataa ggggcagaaa ttttgcccta atctgcccaa
     4141 tatttcggag gataaatgac aagcacagct cactattcgc cagaacaaca acttttgatt
     4201 gatggaatgc actgtgccgc ctgtgttcgg cgagtggaaa aagccttact taaagtggaa
     4261 aaggtggaat ttgcctcagt caatcttgcc gatcaaaccg cttttgtgca aggcaacgcc
     4321 aaccctgaag ccttggtagc agcggtagtt aaaatcggtt ttggggcaga agtgctggaa
     4381 agcgaggaag aacgccgaat taaacaacaa gcccaaacgc aacgagtgtt agggcataaa
     4441 cgcactcaat ttatgcttgc ccttgtagtt ggttttgcct tagttgcttt tgggttctac
     4501 aaaggaatga cggtaaacga aaccaatcgt tcgctttggc tcagctgggc aggtattagc
     4561 cttgcgacaa tgtatttttc aggtaaagat ttctttcttg gggcattttc agcactgaaa
     4621 aaccgctcag ccaatatgga catgttagta gcaatcagca ccggcacggc ttggctctat
     4681 tcttgttggc tcacgctttt cccacaaccg aatgctcacg tttattttga agccagcgtg
     4741 atgattctcg gctttatcaa tctaggaaaa tacttagaac tgaaagccaa acagcgttcc
     4801 tcgctagcat tggaaaaatt gctggatctc gccccaaaac aagcggtcat ttttgaagaa
     4861 aatattgcaa aaaccattcc ggctaaagca attaagccac aaatgcgggt gcaagcctta
     4921 accggtgacc gcttggcggt tgatggtatt ttagacagtg gctctatttg ggttgatgaa
     4981 tcaatgctca ccggtgaagc cctacctgta gagaaaaaaa tcggtgataa agtgcgagcc
     5041 ggcactctgg tgcaagacgg ttcaggcact tacattgccg aacaggtcgg ctcacaaacc
     5101 gctttggctc gtgtaatcaa tgccgttcgc cacgcccaaa gcagcaaacc gccattggca
     5161 cagtttgtag ataaaatcgc cgctgttttt gtgccggtgg tggtttcaat cgcattactt
     5221 tctgcgttaa tttggctggc agttgggcag gattttgcct ttgcactctc gatttttact
     5281 accgtgttga tcatcgcttg cccttgtgca ttggggctgg caattccgct ttctaccatt
     5341 gccggcgtgg cacgttctgc cgaatttggc gtgctggtgc gaaatattga ggcactacaa
     5401 gcaagcagtg aaatcgacac cctagtgttc gataaaaccg gcacactcac cactggcaga
     5461 atggaagtgt cggatgttct cacctttaac ggatttgagc aaaagcgctt gctacaactt
     5521 gctaaaagtg tagagcaaca cgcctcacac ccgattgcga aagcgattgc aaaatttgcc
     5581 gaaaatcaga ccgcttgtgg tgtaaccgat gtgcaagtgt tgaaagggtt aggcatttca
     5641 gcaaaatatg aaggcagtac aatcagaatg ggaaattggc aatttgtagg cgaaaccatt
     5701 caagcaaatc ttgcctccct attgcccgct aaagaggggg atttttcctc aaaaattttt
     5761 gtttcggtgg ataatcaact cgccggtgta ttgtcggttt cagaccaact tcgccctgaa
     5821 gcgaaagcaa ccattcatca attccaagcg gaaggctatc aatgcgtgat gctgaccggt
     5881 gatcgccaac aaactgccga ctatttcgcc aacgcattgg ggttagacgg cgtgattgcg
     5941 gaggtattac cggagcaaaa agcggaaaaa attcgagcct tgcaagcaga gggtaaaaaa
     6001 gtagcaatga ttggcgacgg cataaacgat gctccagctc tggctcaagc caatgtgggc
     6061 attgcgatgc acaatggttc agatattgca gtggaaaccg ccgatctttc gctgatgcga
     6121 gaagggctag caccactgat gcaaattcta ccgttctcta aaaaagtgct gaaaaatatg
     6181 aagcaaagtt tgctcggggc ttttatctat aatgttattg cgattcctat tgctgccggc
     6241 gtgctttacc ctttcaccgg ctggctactc aacccaatga ttgccgccat tgcgatgaca
     6301 ttgtcgtcaa ttaccgtggt gttaaatagt caaaggttgt tgaaataatt taagcggtca
     6361 aatttgcata tttttttgca aatttgaccg cttgtaatca ctaatccgcc aaaaacagcg
     6421 gcccaagagg tgttttcgga atgccaaaat gctcagggta atgcacctcg accaaatata
     6481 gcccctcggc tttcgctgta ggggcagctt tttcccggtc acgttgctcc aacacccatt
     6541 ttgcccattc cacaggctgt ctgccttgcc cgatttcgag caatgttccc acaatattgc
     6601 gaaccatatg atgtacaaag gcgttggctt gaatatcaac aatcacataa ttgccttgcc
     6661 gagacacatt taaatgatga atattccgcc acggcgtatt agattggcat ttggcggcac
     6721 ggaaagagga aaagtcattc tcgcccagca aatactgccc tgcttggtgc attttgcgtt
     6781 catcaagggg aaagtgaaag tgggtaacac ctttaggtaa aattgccgac cgcagttttt
     6841 ggttaaaaat aatatagcgg taacggcggg cggtggcact aaaacgggcg tgaaaatcct
     6901 cattcaccgc taccgcccat ttcaccgcaa tatcatcggg taaatgagcg ttagtgccga
     6961 aacaccagct ttgtagcggg cggtttgctt gcgtttcaaa atgcacgacc tgccctgtgc
     7021 cgtgcacacc ggcatcggtt ctgccggcac aaaacacttc tacgggcgaa ttggcaacaa
     7081 ccgacaacgc ctgctctagc tgttgttgta cactctctac cgcttcttgt cgttgccagc
     7141 caaaataacg gctgccgtca tattcaatac ctaatgcgat tttcatttta ataccttgat
     7201 aaaaagaggg gcgaaaaatt tttcgccccc acagtaattg atatttgcaa ttatttattc
     7261 aataagccta aaaattcttt gcgggtttct cggtcttcta agaaaacacc gccaaaggca
     7321 gaagtaactg tatagctatt ggtgtctttt atgccacggc atttcacgca aaaatgggtg
     7381 gctttcacat acaccgcaac atcgtctgtt tctaaaatag tttgaaaggc agttaaaatc
     7441 tgttcggtaa aacgctcttg cacctgcgga cgttgggcaa aaaactgcac gacacggttg
     7501 attttcgaga gtccaatcac ccattttttc gggtaatagg ctaccgctac tttgccgtca
     7561 atagtaacaa aatgatgctc acaggtagaa gttaaagtaa tatcgtccac cagcaccatt
     7621 tcactgactt tcattcggtt ttcaatattg gtaattttcg ggaaggtagc ataatcaagc
     7681 ccgctgaaaa tttcatcaac atacattttc gccaaacgat gaggcgtttc ttctaagctg
     7741 tcatcactta aatctaaacc gagcaattct aaaactgcct gcatatgctg ctggatttct
     7801 gctcggcgac tggctttatc tttggtatga gtaacagtcg gagtttcaat gccttttgcc
     7861 aacaaggctt ggcgaacttt ttgagcctcc ggcgaaagcg tgttttctgt gatagttaat
     7921 tcgggcgaga tcgttgtcat tggttttcct ctcaaatgta agctgaagat tctaacaaat
     7981 tgacatcaaa aaaaatagct ctaaaatgag ctatttcagc cataaacacc ctctttattt
     8041 atgcaaaata tacagtatct ctttaatgcc gacctgattc ccgaacatca agctgaattt
     8101 gtgcagtctc tcttaacttg caaaaatttt gacaattccg accgcttgct aggcttctca
     8161 aaagggcgag gtacgacttg gtttttagat acctctgctg aactaggtat caatgtggtg
     8221 cttcgccatt attatcgtgg cggcttgttc ggtaaaatca tcaaagatag ttactttttt
     8281 actcgctttg aggccacccg agcgtataaa gaatttttct tactgcaaca aatgttagct
     8341 tggggtttac ctgtgcctcg ccctgtggcg attaaaatag aaagacattt ttgttgctat
     8401 cgtgccgaca ttatgctcga aaagctagaa aacacacaag atttaagcaa aatcttgcaa
     8461 tcccattctt tatcgaacga acaatatgag cagataggga aactgattcg cagtctgcat
     8521 aaccatcaaa ttcaccattc cgacttaaat atccataata ttttgcttgg taatgatggc
     8581 aaattttgtc tgattgattt tgataaatgc ggcattgagc cgggcgaaaa ttggaagcaa
     8641 aataacctag accgcctgtt acgctcgttt aagaaagaac aagggcgatt aaatattttc
     8701 ttcagcgaag aaaattggca ggctgttttg agaggatata ctctctaaga aacaataatg
     8761 gcacaagctt agaaacttgt gccatcaaat catcaatatt aactacttca cttcacgaat
     8821 cataatttca gacggaatca cactgccttg ccagtaaagt tcagaagcta ctttttccgc
     8881 taatgcgagg taggcttggc tgatttcgtg ttccggagct gccaccaccg ttggcacacc
     8941 gctatctaaa tcttcacgca gacgaatatg tagtggtaat tgtgctaaca ctttagtgcc
     9001 atatttttgg gcgatttttt ccgctccgcc ggtgccgaaa attgcctcat ggtgtccgca
     9061 gtttgagcag atatgcattg acatattttc aatcacccca agcactggta ctgagacttt
     9121 ctcaaacatt gagatccctt taatcgcatc taataaggca atatcttgtg gggttgttac
     9181 taccaccgca ccggtcaccg gaatttgctg tgaaagcgta agctgaatat cacccgtacc
     9241 cggtggcata tcaatgacca agtaatccaa ttcattccac caagtttcat ttaaaagctg
     9301 acttaacgca ctgcttgcca tcgggccacg ccaaatggtg gcgttatctt cttccattaa
     9361 atagccgata gaattagatt gcaagccgtg agcggtaatc ggcgtgatgt gcttgttatc
     9421 aggagaagtt ggacgttggt cttttgctcc caacatatgc ggaatagatg ggccgtaaat
     9481 atccgcatct aaaatcccca ctcttgtccc ttgtgctcgt aaggctaaag caagattaac
     9541 tgaggtcgtt gatttcccta ccccaccttt tcctgaagtg accgcaatga tattttttac
     9601 cccattgact gccgggtgtt tattcgcccg ttttaaggtc gcaatttggt aatttaatac
     9661 ccattttaca ccgcttgaac ctgttacttg ctttaattta gcctctgttt cggcttttaa
     9721 ggtttcaaaa ccactgttcc acgcaaatgg catcgtgatt tcaattctaa gaatatcgcc
     9781 ccctaattct gcttttttaa acgcattaag ggcaattaaa tctttctgta aagtcgggtg
     9841 ggaaaattgt tgcaagattg ctttaatttc aacaagttgc tgttcattta attggttcat
     9901 tggttatcct tgtttgctaa attgcgtttt aaatctttgc tataaattac ttcgctatcg
     9961 ttattcaaat caatacccaa aagggaggaa acaatcccaa cccgatgatg gtattgaaat
    10021 aatggcaata cggcaacatc gctataaagc tgctcgctaa tttcttgaat aagtgactct
    10081 ctcgcttctg cactaagcgt ttctgtttgt aaacgattta atttttgatc gactatctca
    10141 cttttatagc cggaatgatt atccgggctc attgaatgaa acttctgcaa aaatacaatc
    10201 ggatcaggat aatctgcaca ccagcctgct cgtgctatct ggtaatcttt ttgttccctt
    10261 actttattta aagtctgcca atcaaccatt ttaggtgtaa tattaaataa ttctgattga
    10321 gaaagggctc gagtaacttg gttagcaata ttaatgctat tctcatcata taacaaagtc
    10381 agctttaaag gattataagg gctaattcct gcttttgtta atagctgctc aaccacaaca
    10441 ggattccaac gcttttctct atcttttact aagctatgtg gaattacaga ctgactcgga
    10501 accccatatt cttgcccact atttgctgaa gccaccattg tccttattgc ttcacgaatc
    10561 tctcgtttag ctaataatgg atcatcaaaa ttgaactcat agtaataaga gcacaaacgc
    10621 ggaaatttca cgatattcgt ttttttattt aataaaggat tctcgacaat atcaaaagca
    10681 gagcagtttt ggatagttgt aattaaatca tactctatag tttgaaaggg aatttcttgg
    10741 gttcgagcct ttaacaccat tttttgagca tctagtttct cgagacggta agacgcattg
    10801 ctcacgaagt tatctatggt tggagcaatc ccttgataag taggtaataa tgcggaatgt
    10861 gccagcattt caggcaattg gtgatttggg tgatttagcc gaattctcaa tgtatgcgca
    10921 tttaatgcct ctacaccgag ttcttcaact gactttattt gctcttcaat cgcttttgca
    10981 ttttctatac ccatatagat gagatattta gacaatacag aatgattttg aggatctatt
    11041 aaacgtcgcc agcttgctac aaaatcttgt gcagtcacag gttggccatt agaccacaca
    11101 gctttctcat ctaaaataaa caaccactct ttttcatctt cagtaaacca attctgagca
    11161 atggccggaa cgacatcacc ttttctgttg taggccgtta aaccgactaa tagatctctt
    11221 aaaggagcag catctgcaga agctttaatt ttatgtgggt cgagttgaaa tacagcagaa
    11281 taaattgcac gtttgagtaa agtatcactc tgccggtttt ctacccagac agattgaatg
    11341 gattgagggc aaattaattg atcacaagat gtaataagca gaatgcaagc ggtaactttt
    11401 gcaatttttt ttacaaatgt aaccgcttgt aaggatttca acataaactc cccaaaggtt
    11461 aattaagatt tattttacaa gaagcacagg aaataaagtt gtaattcctg ccacaataat
    11521 ttcaattccc agcgccatta aaatcagccc catgatacga gtaacgacat tagatcctgt
    11581 tttgcctaat tttttcacca aaggggctga atagcggaat aaaatataac aaattaatgc
    11641 aaatacaata atcgcaatgc taaagccaac atagtcttcc caagtatggt agcgggttcc
    11701 ccacacaatg gttgagccaa ttgcccctgg tcctgccata atcggcattg ctaatggcac
    11761 gacaccgata ttttcgtatt cagtaatatc ttcatttttc tcttctttat tttgtttgtg
    11821 ttcacctaat ttaccgctaa tcatcgtcat cgcaatgctt acaatcaaaa tgccaccagc
    11881 tactctaaaa gagctaagtg aaatgctaaa cgcattgaga atcagattac caaaatagag
    11941 gctgactaat aaaattactc caatagaaac agcggtgatt aagctggtgc gatttctttc
    12001 cccctcatat tggtgagcgg tcatactgaa aaaaataggc aaggaaccaa aaggattgac
    12061 tatagcaaat agaccaatga agaattggag gtagatggca aaattaataa tgatatccac
    12121 aaaaactttc tcaaacaaca gtgtttcggg cgctattata tcggttaatc aaggtagata
    12181 caaaccaaaa gcacccagcc taaaggacgg ttgggtgctt tttgataaaa tgaaaggctt
    12241 gttagtgtga cgaacgttgc tgcataaaat cttcatttac ctcaattgaa gcttttgcac
    12301 gtagcgattg taagaattca ttacgtaaaa ttaaacgatt agcttgttca aattgtggcg
    12361 ccaatgtctt aaactgttca gcattccctt ccgtaatgtt atctaaggca acaataacta
    12421 catcgccctt ttgattacgt gccatttgat aagtcgtttt atcttgcgcc tttttcattg
    12481 cgaaaatcgt tccagccaat accgggtttt ctacttctgc ttgcatataa actaaagttt
    12541 gtggcgcatt aaatttcacg tccactgatt tcccttcatt caaggctttt acattttctt
    12601 ctactttcgc cagcaacgct ttttccgctt tttctgcctt cacaaaattt tcaactgctg
    12661 cttttgcttc atcaaaagtt tgtggacgtt ctgcatcata atcactcact ctcacaaaca
    12721 atgtctgtgg atttgcatca ttcccaacat ctaaagcatc agaattttgt ttattttggc
    12781 gtagctctcc ttcaaacaaa acttttaata ctttctcatt ttgtaaagca gtaggtacgt
    12841 tttcacgggt aaacagatcc gttttttgca gttccacatt accggctttc gcaacttctt
    12901 caagtgaaga attattctca aaggctttat ttgccatttc gcgcgcaact gtaaaatact
    12961 cagttaacac taattcttgg cgaatagttt gggcaatttt atctttcact tgtgctaact
    13021 caatcacttg ctcaggttta cgctccaata ctttgatgat atggtaagca ttatccactt
    13081 tcaccgctgc actcatctgg ccggcagtta aatttgctac agcatcttca aaagctttcg
    13141 ggaaagtgcc agcttttgcc caacctagat caccaccatt atttgctgat aaagtatcgc
    13201 tagatttgtt ttttgccaaa gtagcaaaat cttccccatt tttgagcgct tgctcaatag
    13261 cctgtgcttc cgcttcacta gcgacttgaa tatgagctaa atgggcttca cctttagtta
    13321 cataatcggc tttattgcga tcatagtaag tttgaatttg ctcattggta atctcaactt
    13381 tgctttccac atcttttgga gaaagagtaa cgtactcaac cgctaatttt tcaggtgcta
    13441 ataatttagt tttatttgct tcgtaatagg ctcgcaattc ttgctcggaa gcggtctgat
    13501 tttccatttc ttttgcaact gaaaattcgg ctaaacgcac ttcacgttgt tgcaacagta
    13561 attttgctaa caatgcctgc tgtgccggta cattaaaatc actattgata atcccctctt
    13621 gtagctggga ttgcaacata ccttcctgca caatcgctgc atagccatcg gcacttaaat
    13681 tattgtggcg taatgtttgt tggtataact cgttactgaa tttaccatct tgttgaaaca
    13741 tctggctgtt tacaatttca gatttgattt gatcagcact aatacctagt tttaactctt
    13801 ttgcatactg gcgcagaagc tcttcatcaa ttaatccatt taataccgat tgattaaatt
    13861 gagcagcata ctccgggcta tccattaaat cccaaaagcg actccccagt tcagcattta
    13921 atctattttg ctcacgatta agcgtgttat taaataattg ctgactaatt tcttccccat
    13981 ttactttcgc tacagaatta tcttgtgaaa tcaatcctgt cccaattcca ccgattacaa
    14041 atgaaataga gactaatgca aaaataattt taaagaccgg cccatttgtc cgctcgtgca
    14101 ttttttcaat catttaaaaa ttcctaaatt actcaaaaat tgtcgctgat tatactcttt
    14161 ctcaaatgag aaataaactt gcaaattttt attgaaattt aaccgctttc tctttaaaat
    14221 tcacagtccc tgtcgtagct gagttagaga tcggctattg atgtattatc aacccttaac
    14281 aaaaggaaat taaaatgtct ctaagcgtag aagcaaaagc aaaaatcgtt gctgaatttg
    14341 gtcgtgatgc aaaagacact ggttcttctg aagttcaaat tgcattatta actgcacaaa
    14401 tcaaccactt acaagcacac tttgctgagc ataaaaaaga tcaccacggt cgtcgtggtt
    14461 tattacgtat ggtttcacgc cgtcgtaaat tattagacta cttaaaacgt actgatcttg
    14521 ctaaatattc ggaaactatc gcacgtttag gtttacgtcg ctaatttcaa atattagata
    14581 tgagagccga gctgaaaagt tcggcttttt tataaatttt attcgaggaa aaaatatggc
    14641 ttttgcgtca ttatttacat tattagacga tattgctgcc gtgcttgatg atgtatcgat
    14701 catgactaaa gtggctgcta agaaaacagc aggcgtaatc ggtgatgatc ttgccttaaa
    14761 tgctaatcaa gtcagtggaa agaatgtggt gccagaacga gaactaccga ttgtttgggc
    14821 tgtagcgaaa ggctcattaa tcaataaagt gatcttgatt ccactagcgt tgctgctatc
    14881 ggtcttttta ccgcaggcta ttatcccgct attaatgata ggcggtgcat atttatgttt
    14941 tgaaggcgta gagaaattac tccatcgtct attacataaa aacaatcaag ctcattctga
    15001 tgaaaccgaa accctttctg aaaaagataa aattaaaggt gcaattcgca ccgattttat
    15061 tctatcagca gagattatca ttattgcgtt aggtgttttg caagactctt caaccctaac
    15121 ccaaatttta tctctctccg ctatcggggt cggaattacc ctgtttgttt atggcttagt
    15181 tggattaatt gtaaaactag atgatatcgg tttatggtta atgcagaaaa aaagcacatt
    15241 ctcacaagcg gttggaaaag gcttattatt tgcaatgccg tggtttatga aaggactttc
    15301 tgtgattggt actattgcga tgttcttagt cggtggtggt atttttaccc ataatttacc
    15361 aatgattcat cacttcgtag aacaattcgg tatctctccc catttttcta ctctggttga
    15421 ttttgcagtg ggtattgtgg ttggtattat tgcatgctta attattttac ctttgatgaa
    15481 attattttct aaaaaggcgc actaatcccc aaaagcgtag ctttggttaa agctagcttt
    15541 ttatctcttg ctataaaaaa atagcaggtt tcaagcctgc tattttttta attattataa
    15601 tgagttagac tcagtggctg tttgcggtaa ggctaatggc tcattaactt tagactgagt
    15661 agccatattc acctgtttga gattttcctc acgaatacgg tttgccatta atgtatcccc
    15721 catctgctct gccacgtgca gtgccataat acgatcatca gcaacacatt catcatgagc
    15781 caataacaat tcaaactgag acttcgcttt ttggtattgc ccatctcgtg tataaatata
    15841 gccaagcgct ctagcataat catcggtata tttaacattc gctttatctg cccgttttgc
    15901 taatactttt agtagcttac tgtcagaatc aatttgtaaa cgggttaatt gcacaaataa
    15961 accttgtaat tgctcatctt caaatttctt caacgtttct aaagcaattt cttgtgcaga
    16021 ttgatgatcg tcactatcaa ttaaacgctt gatgacaccg acacgactat acaccgattt
    16081 acgacgacga tttggttggt tttcccacca agaaagcaag ccttcctgcc cttcttcctg
    16141 taaacgctca tcttgtaaac catcatcaac gaaatactct aactcagcat actcttctgc
    16201 agttaagaaa ttacgttggc cgatttcaac taataatcta tctaacgcaa cataagctct
    16261 ggattcttta taaatttgaa tcgctaagcg taatacctca tcgctatgag gtgcaagctc
    16321 tagtaggcta tcaatcgcag ttcgggcagc cggtaatttt ccttgttgta ataaaattcg
    16381 ggttcgtacc aactccactg caacattatt cggtccagct atttcagttg ccttaattaa
    16441 atatttattt gcagctaaat catcaccatt ttgctgagca gcttccgccg ctttaattaa
    16501 attcagtaca ggctcatctg catgttttgc atttttacta aagagttttt ccgcttttga
    16561 gtaattccct tcactcatac gcattaaacc ttccagtgtt tcttgctgag cttttttatg
    16621 cttacgagtt gagaaccagt tataagctcc attactcata cggcatattt tagtgacaat
    16681 ccattcaagt aaataaacta ccgccattgc caacacaaaa aaaaccacaa gcattacaat
    16741 gctcatttca atcacggtag ttgctgtttc aattctgaca taaccttgat ttccggctaa
    16801 ataaggccca gagattagtc caactaataa taaaagcatc aaaaagagcg ttctaaacat
    16861 aatctattct cctttttatt attgtgctga aagaggcatc gcatcttgct ctgcttttac
    16921 aggctctgct tgctctaatt ctttttcagc ttttatttcg actttctcta ttttttcagg
    16981 ggttttatta agttgctgag caatcacctt taagctttgt aatgaagttg gagcatccac
    17041 ataaatagtt tgttctgcca actcatctag ctctttcaag aaatgttgca cattttcatt
    17101 tgaagtttca aaataacttc ttacccaaga gcctaccgtt tgtaacgatt ttttataaag
    17161 ttcgctttgc tggcgaggaa cagctaaaat cgcaatttgt aaacgcaagc gaatgttttc
    17221 acgcaaataa atttcttgat tcggtgcaac aaaggctttc tcatctgctg cattacgttt
    17281 cgatacacga ataaaatgat ctaaaaatga agcagcactt ttttctaaat ttttctccca
    17341 atccgcaaga gaatcactta cttgcccatc ttgtttgcta tttgaagctt gttcactttc
    17401 caaaatcggc aaatcatcta atttattcgc taaatttgcg acacgctgca ttaaggcatt
    17461 ttgatcgatc tgatgcaatg cggttaatgt atttaaatca gttttgattg cctcacgaat
    17521 gtgaatcgta ttcggtgctt ttgataaaac ttgatctgcc tctaacaata aatttttcgc
    17581 tgtttcaatg tcattatcta acaccacttt acgagccgca ttattcaata ggaaatctgc
    17641 ctcagataat agccagaatg tgttatccga accagaggta ttatttagct tttgcagttg
    17701 aagctgcaaa ctactaattt gctgggtata accattttgg gctgtttcta aagctttaat
    17761 ccgttctaaa gctttttgat attccgtgac caattcggca atttgtgctt tttcattttc
    17821 gaaatttggc atctcaacca tagtttgagt agtaggctga tttgctttag cagatactgc
    17881 ctgaatttga gattcaacct ctgcaaactt tttatttgcc atataatgac cagctccgcc
    17941 aaccgctaaa gccactaata gtgcaactaa tgccaagccc tttccgcccg atttttgaac
    18001 aaccacttgt tcttttggcg ctttttgcat ttctgtttga atatctttct ctatagcttc
    18061 gttcactaca tcttcctcaa tctctttttt tgcaaaagat cccgctgaat cgaccgcttg
    18121 ttgtgcctct tctaccacct tttcagctga atgctctaaa tgttcagtta cttttttaga
    18181 tcttgacata ctcgccacct ctagttagat ttgcccactt taaataataa tgtattcatc
    18241 aaagttacat tatcggcttt atccgatacc ctaatcttat cttcttgcca gcccatttgt
    18301 ttagctatct ttgcaatccg ccggctcact acaaccagat cacattcaaa taaccatgct
    18361 ctacaatctg ctttcgcttg ttcataaagt gatcttaaaa tttcactact ggtgatcaca
    18421 atagtatcta ctcccaagcg tttacataag ctgatttttt cgggaatatc atcggtaacc
    18481 ggctctctac gataacactc caaatattga atagttgccc cacgcaagac tgcagtttca
    18541 gcaagtagct cacgcccaga atcggctctt agaattaaaa tagttttatc agttagattc
    18601 tgcatttcag gtaatgccaa aaccccctcg ctattttcaa attctatcgg ataaatgacc
    18661 gcttgttcag ctttttctgt taaatatttc gcagtccgtt gccctaccgc aaaatatttc
    18721 aaatctgaac gaaaatggaa cccggtatct actaatgttt ttgcagcata gtccactgca
    18781 ttcggcgaaa caacaaatac ataatcgtct gattttagcc gagaaatagc cgaaggaagc
    18841 aaaggtaatt ctttaccggc ttcaatcgta aatagcggtt gatgaatagc aaaaatttgc
    18901 tgttcattca acatatctac caattcttgc ccccgactat ccaggcgtgt gaccaacaca
    18961 ttcattattg tacgttttcg ttataaaccg cagctaaaat tttatctgca ccttgagcaa
    19021 gcaactgttc cgccaccaaa atgcctaatt gctccgcctc ttgcttatgt ccttttgccg
    19081 aagcccgaat aatttgtgag ccatctaacg atccaactaa cgcattaagc gttaattcat
    19141 cgccctctaa tgtagcataa ccaccaatcg gcacttggca accgccttgc aaacgggtat
    19201 tcattgcacg ttctgccatc acgcaacaag cggtcgaact atggtttaat tttgcaatgt
    19261 agttcaaaat ccgttcatca ttggctcgag tctcaatccc tactgctcct tgaccagctg
    19321 cgggtaatga ttgttccaca gaaatatagc tacggattcg ctcttgcaag cctaaacgaa
    19381 ttaagccggc agaggctaaa ataatcgcat catactcgcc attatctaat ttgcttaagc
    19441 gagtgccgac attgcctcgc aatgatttaa cctctaaatg cggatatttc gccattaatt
    19501 ggcactgacg gcgtaagctc gatgtaccga ccacagcacc tttcggcaaa tcttctaacg
    19561 aatggtagtt gttcgacaca aacgcatcac ggggatcttc acgttcacaa atcaccgcaa
    19621 gccccaagcc ttcgggaaag ctcatcggca catctttcat tgaatgcacc gcaatatcag
    19681 ctcgattttc taaaagggct aattctaact ctttcacaaa caagcctttt ccgccaattt
    19741 tggctaatgg ggtgtctaaa ataatatcgc ctttcgttac catcgtaacc agttcgacag
    19801 ataattgcgg aaaatgtttt tctaattcgc tttttacaaa atttgcctgc cataaggcta
    19861 acgggctttg acgggttgca atgcgtaaaa tatctttcat aaattctaaa attaaaacat
    19921 tgagttaatg aattatccta ccacttttta gctgaactgc caaaaataat cgtggatttc
    19981 gctttgcatt taatcaaaaa tccgactttt tctcaaaatg tatcaatgtc
//
LOCUS       DANLXQ010000028        19992 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.028, whole genome shotgun sequence.
ACCESSION   DANLXQ010000028 DANLXQ010000000
VERSION     DANLXQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19992
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.028"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..574
                     /locus_tag="PXD94_000789"
     CDS             <1..574
                     /locus_tag="PXD94_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL6285677.1"
                     /translation="THFYREKYLSDQYNFLALYPQDKTQICQKFQKEQISQADLSDKQ
                     FRFLQYQFACQFHSLFKDKKPTKEKYIHFTHLADYLDLSNVPNQEIYTIHSLAELPSS
                     SEREPKIVLAKNAINENLPQEFYGIVITDYLFDITGKKMYGTLYSSYDNAREERNLTF
                     KKEVLAELEARYSAWRYLPYSEHLMGAVDD"
     gene            567..860
                     /locus_tag="PXD94_000790"
     CDS             567..860
                     /locus_tag="PXD94_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL6285678.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            909..4199
                     /gene="recC"
                     /locus_tag="PXD94_000791"
     CDS             909..4199
                     /gene="recC"
                     /locus_tag="PXD94_000791"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL6285679.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTMDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            4335..7280
                     /gene="ptrA"
                     /locus_tag="PXD94_000792"
     CDS             4335..7280
                     /gene="ptrA"
                     /locus_tag="PXD94_000792"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL6285680.1"
                     /translation="MKLSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLG
                     FELIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQ
                     GLAHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAV
                     TRLADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAV
                     GNKETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENK
                     HFSIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLN
                     NNTDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVF
                     QQIEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQP
                     YLTETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKW
                     LDFSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPK
                     AKIGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLAL
                     SAEGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALS
                     RFASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAED
                     LQAVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRA
                     KLLRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNER
                     FFTQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQ
                     VIELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKL
                     QREFEIKDY"
     gene            complement(7329..8975)
                     /locus_tag="PXD94_000793"
     CDS             complement(7329..8975)
                     /locus_tag="PXD94_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6285681.1"
                     /translation="MKFPTLTKISASVLAVLALAACDDKSNENKPTAAAAKPQTEKTF
                     VNCVSRSPTGFSPILMMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGL
                     TYTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMK
                     FPTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDT
                     TPIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCD
                     LMDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKA
                     IIDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQ
                     PVVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDN
                     GDPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALGSSDKAERTKLYEQAQVILRE
                     QAPWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            9051..11141
                     /locus_tag="PXD94_000794"
     CDS             9051..11141
                     /locus_tag="PXD94_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL6285682.1"
                     /translation="MIFANQSIELDTVNVIATRDPSKFADTPQKQTKDALLVKQATSV
                     AAALKALPNVDVQGGARAIVQKPSIRGLSDNRIVQVIDGVRQNFDLAHRGSYFVPMSL
                     IQEIEVIKGPSSSLWGSGALGGVVAMRTPNALDLLKNNDKFGAKIRQGYQTANNLSET
                     EASVFAANDRFDALLSGFYNNADNLRSGEGKELNNTGYKQAGGLVKFGWQINDTHRLE
                     LSHRHSQFKQTAPGNNEVENQFTDNDVSAQISAWHAANPGNPSNPVYLAKMLAFYGQL
                     HSSLGSVSYLSDQKITDKSTSLNYYFNPSNPYLSTQVTLYSNSTTENEHRKISGVEDQ
                     TKLSTRGVNIRNHSELSWFSLVYGVDYMLDKVATQRGRNNTDAQYRANPYDAKSKTTG
                     AYLIAHIPLFDEKVVFSPSVRYDRFDTSSETVKYKDSHWSPAAKLTWKATNWLDLTAK
                     YNEAFRAPSMQERFVSGSHFGTTVRGLPINNIFLTNPNLRPETAKNKEVTFNVHFDNV
                     ISQGDKFNFEATYFRNDVKDLINLQVFKANPTSPMEFIPTRSQYQNIANARLSGIELQ
                     TRYQTERLAVFANYGSTKGKDKDSGEALSNIAASKIGFGVNYAVVQDKFTVGANVTRY
                     QAQHRVPKNHGVTYQGYTLTDLHATYAPLKGEWKNLRLDVAVENLFDKKYQPAFSLME
                     GSGRNVKLSAAYSF"
     gene            11212..12900
                     /locus_tag="PXD94_000795"
     CDS             11212..12900
                     /locus_tag="PXD94_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL6285683.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAVNVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDRTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHNLGAYVLHPTIRLPDFRLNT
                     KVSFNHQRLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQAHYVATDFQPKSRFTVYQYSLSHEQALPKSFAFNLGVNGQFADKTLDSSQKMLLG
                     GLYGVRGHQAGVASVDEGHLVQTELKHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLA
                     RGVNNSVKLQSIGAGLSLSEAGSYAINASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            12912..15728
                     /locus_tag="PXD94_000796"
     CDS             12912..15728
                     /locus_tag="PXD94_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL6285684.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTKDKFSRKLAKEGKILNQGKIT
                     AKGERAFVVLVGDDVANEGEINAKNYYHKETKKVTECYDDSWSLVKQCYDFDKEIVKS
                     TSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNDGAIIANGDITLTAKGRNQAL
                     DSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYGPTEDIKV
                     VSKTGSKVTAPVIDFKGKTVNIQGEFGREDSTDLYNDELQILKTTVNIEVPNTEDIRI
                     ADTDNGGSGSFIQAGALASLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFINQA
                     GINIHNADIHANGRLFFVTNLPNDTDFQSNIRITDSKINLGNGSMGFGRSSKRWEMHD
                     VVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGNSR
                     MFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDETDLNWEYNQRFDGFAAKHNH
                     RAPIADTEINIANSDISLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRLGL
                     NGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTDSN
                     VTIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNATA
                     IVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPDKL
                     TPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDEQN
                     KCEERMLGNKNTNAKVIVGDIKFE"
     gene            15851..15982
                     /locus_tag="PXD94_000797"
                     /pseudo
     CDS             15851..15982
                     /locus_tag="PXD94_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16037..16798)
                     /locus_tag="PXD94_000798"
     CDS             complement(16037..16798)
                     /locus_tag="PXD94_000798"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL6285685.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTVQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNELVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(16810..17841)
                     /gene="murB"
                     /locus_tag="PXD94_000799"
     CDS             complement(16810..17841)
                     /gene="murB"
                     /locus_tag="PXD94_000799"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL6285686.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVASSAPIQNIGAYGVEFEQVCDFVEVLNLRSGELFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPIQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQAKFPAIPHYPQADGTVKLAAGWLIDQTGLK
                     DLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVKEKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(17945..18619)
                     /gene="crp"
                     /locus_tag="PXD94_000800"
     CDS             complement(17945..18619)
                     /gene="crp"
                     /locus_tag="PXD94_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL6285687.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKGPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIIKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(18681..18899)
                     /locus_tag="PXD94_000801"
     CDS             complement(18681..18899)
                     /locus_tag="PXD94_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL6285688.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(18902..19507)
                     /gene="slmA"
                     /locus_tag="PXD94_000802"
     CDS             complement(18902..19507)
                     /gene="slmA"
                     /locus_tag="PXD94_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL6285689.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAA
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(19519..19974)
                     /gene="dut"
                     /locus_tag="PXD94_000803"
     CDS             complement(19519..19974)
                     /gene="dut"
                     /locus_tag="PXD94_000803"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL6285690.1"
                     /translation="MKQIDLKILDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKA
                     GETVLIPTGISIYIADPNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKVSLWN
                     RSQADFTVNVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
BASE COUNT         5987 a         4168 c         4361 g         5476 t
ORIGIN      
        1 tacccatttt tatagagaga aatacctttc cgatcaatat aattttttag cgttatatcc
       61 acaagataaa acgcaaatat gccaaaaatt tcaaaaagag cagatcagcc aagcggattt
      121 atccgataaa caattcaggt ttttacagta tcaatttgcc tgccaattcc atagtttatt
      181 taaagataaa aaaccgacta aagagaaata tattcacttt acccatttgg cggattattt
      241 agatttatca aatgtgccaa accaagagat ttatactatt cattctttag ctgaattacc
      301 tagtagcagt gaacgtgagc ctaaaattgt gcttgctaaa aacgcaatta atgaaaactt
      361 accgcaagag ttttacggca ttgtgataac cgactatctg tttgatatta ccggcaaaaa
      421 aatgtacggc acgctttatt cctcttatga taatgcccga gaggagcgaa atttaacgtt
      481 taaaaaagag gtactggcag agcttgaggc gaggtattcc gcttggcgat atttgccata
      541 ttccgaacat ttgatgggag cggttgatga ttaaatataa agcagaaacg gctttgtcgc
      601 ttttggttgc catcacgcta tttgcgattg tggtgttgag tttcagccag tggcaatcgg
      661 cacaaaacca caggctaaat caacatttcc aacagcaaca agcggttatg attttagaaa
      721 atcagcttgc cctcaaatta gccgggttag attgtgaatc aagtattact caaaatcaga
      781 ttcgctatca gattcaatgc tctggcaatc gcttaaccat tcgctttccg ttaggcaaaa
      841 ttgagctgaa taatgattga atcgtccctc gctttggagg acaataattc aattagcgag
      901 gcgttattat gtttacactt taccaatcca accaaatttc atcactggcg gagatgctgg
      961 tgaaaatcca gcaggtaaac ccacttgaag atccgtttga gccggaaacc attttgatcc
     1021 aaagtcaggg aatggcacag tggctacaaa tgcagattgc tgaattaaac ggtgtgatgg
     1081 ggaattgcga ttttctctac cctactacat ttttatggca acagtatcgg ctgttgttcc
     1141 cggagctgcc gaaagagaat atttttgagc gtagttcgtt ggtttggcgg attatgcggt
     1201 tattgccgag ttgcttgaca ctatcggagt ttgctccgct aaagcgttat ttaggtgatc
     1261 aacctaataa agaacagtat cagcttaagc tctatcaact ttctgccaaa attgccgaat
     1321 tgttcgacca atatttggtg tatcgccccc attggctggt tagctgggaa gagggcaact
     1381 tacaagcgat cgaaaatgag ataaaaattg caactgccgc taagccgatt gattttgact
     1441 taattcgagc cgatatgaaa tggcaaagcc atttatggca acagattgta tctgatctgc
     1501 aatcggaatc tgatgagcag gtttttacta cctcccaccg tgcctatttg caacaaaatt
     1561 acttcgctaa gttagatgac cttactgaaa cggaaaaagc caaattgcca aaacgcattt
     1621 tcatttttgg gatttcgtcc ttctcgccat tacagttagc ggtgtttaag aaactcagcg
     1681 agcattgcga tattcacctg ttctttttta acccaagcga aaaatattgg ggcgacagtg
     1741 tggaagaaaa agtgtggcaa aaattggcgt taagccacaa gatttcgcaa gacgatctgg
     1801 ataacttatt ggcggagcaa agaaataaac tactgaatct ttggggcaaa caagggcggg
     1861 atttcttagc tcaattaatc gaatttgagc cgaataccat ggatgtcttt gtcgaaccag
     1921 aggaaaacag caacctcaat aaactcaaaa aacagatttt cagcttagaa aatgacgcca
     1981 ctattaccga tttaaccgcc gataattcac tccaaattca ttcgtgccac agcccaatgc
     2041 gggaagtgga agtgttgcat aaccagctcc tgaatttgtt tgagcaagat agcactttgc
     2101 tgccgaaaga tattattgtg atgtcgccgg atattaataa atacgctcct tacattgatg
     2161 cggtgttttc tcgctacaat acgggtaatc gggatcctcg ctatattcct ttttcattat
     2221 ccgaccaagc atttacgcag gttgatccga ttatccacag tttcctgcaa ctgctggcaa
     2281 tgaaagaaag cccgtttaat gccgaagaac tgtttgattt actggaagtg aatgcgatcc
     2341 aaacccgttt tggctttagt acggaagata ttcataccct gcgggtttgg gtgagaaatg
     2401 ccggcgtacg ttcgggtgta gaaatcgagc aacctcactg gcaaaactac aattcgtggg
     2461 aaaacgggct aaaccgttta ttgcttggct caagcctgaa agaagagcag gggatttggg
     2521 aaaacaccct tgcctttggg gatagctatg gcttaaatgc cgagctggtg gggcgacttg
     2581 cctcttttat tgaggcttta ttagcgtggg taaactttct gcaaacgcca cagccgttgg
     2641 cagcgtggca gcaggcaata aaaaatttga ctgctgatct gtatcaagaa aatgccgaaa
     2701 atgctgcatc aattttattg ttgaataatg tgaatgatca gattatcgag caaatttccg
     2761 caacccattt ttcagacgat ctgcaaattg aggttttagc cttattgttt gagcagcagc
     2821 tcagccaaca ttcacagcag cttaatttct tggtcggacg ggtgaatttt gccaccttgc
     2881 ttccgatgcg ggcgattccg tttaaggtaa tttgcttgct gggtatgaat gaggcagatt
     2941 tccctcgcca acaacaaatc aatagctttg atttaatgca gtacgccccg aaaaagggag
     3001 accgtgccaa acgtgatgat gaccgctact tgttcttaga agccttactt tccgctcagc
     3061 aaatgtttta catcagctat atcgggcagt cgcaaaaaga cggcaaagag atgctaccgt
     3121 cgattttggt ttcccaactg ttagatgcaa tgaatgaagc tttaacgcct gaatgcaagc
     3181 aacaattaag cgatgaagtg gaagttttgg tcagaaaaca acctttaagt gtgtttagca
     3241 aaaagaattt tacccaccca cgagatcacg cttataacgc agagtggatt atcaagccga
     3301 ctgttgccgt acaagatttt cttagtgagc ctttaaccaa tgaaagcgtg gcttcgctag
     3361 aacttgatga attaatctct tttgtacaaa acccgattag gtttttcttt ctaaaagtgt
     3421 tggggatttc gtttaacact tatgatgaca ccattgatga aagcgaaatt tttgagatgt
     3481 cggggctgga taactaccgg attttaaacg aattattaac gataaattta gcacaacagc
     3541 aacgtttttt tgccgaagag caactcaaag gcaatttacc tgcttcttat tttgctcaaa
     3601 ttgccacaca agatttgatc ggcaaaatta ccgaaatgca agcggcgtta agccactatt
     3661 tagccagcga aagccgtatt ttgagtgtta atcgggaggt tgagctggaa cgccaaaccc
     3721 tcaccctaaa cggcaatatt gccaacctat atcagaatga agtggtgcta tggcgagtag
     3781 ggcatttacg agataaagac aagattcaga cttggatcta ccatttatta ttgtgtgcct
     3841 atttccctga aaaaacggtg aaattctatt accgagaggg tgagcaagtt ggtgtgttga
     3901 gctttgaaac cgtctcgcag atagaggcat tatcgcaatt aagcatttat ttgaatgctt
     3961 atttggacgg attatcgcaa gcccagttag tgatctacga ccgaattgag gaatatctca
     4021 aaggtgatgg cacaaacagc gaacaagttc ttatcgattt agcagagaaa gatgagcgag
     4081 acggcagcta tctccatcgg cttctcgctc aaactaaaga gctggattct caagcgattc
     4141 accaacgtac tcaaagctgg tttagcttaa tgaatcagaa aatagcgaaa ggcgattaac
     4201 tacaagcggt cgtttttggc tgaaaatttg caaaatgaga aggcgaataa taattcgcct
     4261 acctataaaa atagaatttt ttccgtattt tactgtcaat ttattactac aatgctaatt
     4321 tcaaaaggag atgtatgaaa ttatcttttc ttacccaatt cgttgcagcg gcttttgccc
     4381 tcaatgtgat gacttaccct gcatttgcgg aagttgcaaa aaattctgaa aaaacgaccg
     4441 ctagtcaacc ccaatcccaa ctcggttttg agctgattaa acagagcatt aacaaaagcc
     4501 cgaatgataa agcgatttat caaggtatta aacttgataa tggaatggaa gtgctactga
     4561 tttccgacag tcaagcgaat aaatcgttga tgtctgccat tattccgatt ggctcaatgg
     4621 atgacccgat ttctcagcaa ggtttagccc actatcttga gcatatgatc ttgatgggct
     4681 cgaaaaacta tccggaaacc aacagcttag acggtttttt aaccaaaaat ggcggtatga
     4741 ataatgcctc gactgcttct caccgcacgg cgtattatct acaagtcaat aacgaagcct
     4801 ttagcgaagc ggtaacacga ctggcggata cgttagcttt cccgttattg ctggaaacca
     4861 acgccaagaa agaggttaat gcggtcaatg cggagatggt gcgagctaaa tcgcacgatg
     4921 ggcatttatt gcacagtgtt aatttagcca ccgcaaaccc ggctcatcct gctaccaaat
     4981 ttgcggtagg caataaagag acgttatctg acaagccgga tagcaaatta caaaccgagt
     5041 tggagcaatt ttataaacag cattattctg cgaatctgtt taaagtggtg ctttattcca
     5101 atcaatcgat tgagcagatg gcaaaattgg cagccaacac tttaggcaaa atggaaaaca
     5161 aacatttcag cataccgcaa accgctgagc cacgttatcg tgatgaggac aaatcggtat
     5221 ttattcaata taagccggtg aaatccaaca aaatgttagt cattggcttt gattttccga
     5281 atgacgaaat gcagtttaag cacaaaaccg gtaaatatat tgcttatatg ttaaacaata
     5341 atactgacgg cacgctttcg gattacttga ttaaacaggg gttgtctgac ggcggtattg
     5401 aggcggttag cagtgccaat atcgggcggg atcgaggctc gtttgacatc tatattgaac
     5461 tgacagacaa aggtttgagc gagcaagatc aaattatttc attggttttc caacaaattg
     5521 agaagatcaa acaagaaggc attcaagaga gctattttag cgaagtgaaa gagagcctga
     5581 agcaggattt ccaacatttg caagtggaaa aaaatatgga tttcgtggaa tttattgccg
     5641 atcaaatgct ccatttcccg ttagaacacg ttatcgatca gccttatctg accgaaacaa
     5701 tggataacga ggcaattaaa gcaaaattgg cggcaatgaa tattgataat gcccgcatta
     5761 tgttagtcag cgatactgcc aaaaccgaca agaaaacccc ttattttgaa gcaggttatt
     5821 cggtggcaaa aatcacgcaa gaacaaaagc aaaaatggct ggatttcagt aaaaatccac
     5881 agctcaattt gcctgaattg aacccgtatt tcactaccga ttttacctta aacaaggtcg
     5941 aaaccggctt aactaaaccg aaattgatta acgaccaagc ggggaattta atctatacaa
     6001 tgccgagcgt gtattttacc aatgaaccga aggctaaaat cggcgttggc tttaatattc
     6061 gcccgagaaa agatgactta aagagcctga ccgcagcgtc tattttgggt tatatgaaca
     6121 atttagccca aaccaagtta gattttcagt cttcggtggc gggtatgaat ttaagtttag
     6181 ctccggcggc aaacggttta gccttaagtg cagagggtta tacccaaaat ctggctaaat
     6241 taatgctaga cagcgtaaaa cagttcagcc gctttgagtt taacgaagac gtgttggcac
     6301 aggctaaaca acgtttgcac caagagctgg atcgtaacga aaaagaaaac agccttaatc
     6361 aagctaatgc agcattaagc cgttttgcct cttaccctta ttttgatgtg ccgaaacagc
     6421 gtgagatgat tgatagcatt acccttgcgg atattgagca actacgccag cgaattttgc
     6481 agcagagcag cggctttaag ttgttatcgg tggggaattt gtcggctgag caggtttctg
     6541 ctctggcaga ggatttacaa gcggtcataa aaaaccaaaa atctgcaaat gctaccggac
     6601 agtatgtgga tgtgagtacc agtcagcgta agattaatgt gattaaaagc gtgccgaatg
     6661 aagataatgc gttgctaatg gcatttatgc cgaacggcta taacgagctg gaaagcctta
     6721 ctcgagccaa attgctcaga agcattattt cccgttggta ttttgatgat ttgcgtactg
     6781 ataagcaact aggctatgtg gtttatgcga cagataatat catcgggaaa accgccggta
     6841 tccagtttat ggtacaaagc ccgaatacca caccggcagg gattttagag cataacgaac
     6901 gtttctttac acaaagtttc gaacgtttaa aaaatctatc tgatgaggaa tttggaaaat
     6961 atcgtgatag cctagtggaa aaactgcaat ataaaccgga gtctttagcg gaagagtttt
     7021 ctgagttcgg ttcagacttt agcagaaata acccgaaatt tgaccgcttg caacaggtga
     7081 tcgaattaac caaagcactc accaaagcag atattgtggc attttaccaa aaagctgtga
     7141 ttgaaaagca aggcttcgtg tttgtcagcc aagctgtcgg caccaaaacc aaagctgaag
     7201 acgttgcgaa atttgacggt tttgaacaga tcgaaagcat tgaaaagcta caacgagagt
     7261 ttgagattaa ggattattag agagttatta gagagagtta ggggcgaaaa attttcgccc
     7321 ctcatttttt aatccacaat tttcaccgga tataagtaag tatatccgaa cggactttgt
     7381 ttgtagcctt caacacgttt gctggttggg gcaaagttaa ttgagtgagc cacgttaatc
     7441 cacggagctt gctctcttaa gataacctgt gcctgctcgt ataacttagt acgttctgct
     7501 ttatcagatg agcctaatgc tttttctaat aacgcatcta actcagggtt agtaaagcga
     7561 gcatagttac tgttgccgat atttggcgtg ccgaatagtg gcgataagaa gttatccgga
     7621 tcgccgttat cgcccgacca accgtaagtt ccggcggtta attcaccggc tttggtacgc
     7681 ttgatgtaat caccccattc ataggtgact aattttgctt ttacgccgat tttttcccag
     7741 tcggcttgaa taatttcaga catacggcgt gggttcgggt tagaagcacg caccacaggc
     7801 tgcacccata attcggtttc aaaaccgttc gggaagcctg cttcagctaa tagttgcttc
     7861 gctttttcca cgttatattc tgaatccgct aagctgtcgt tatagcccca aatggttggc
     7921 ggaagtgggt ttttcgccgc aaaaccgtta ccttggtaaa ctacatcaat aatcgctttt
     7981 ttatccactg ctaagtttaa cgcttggcga actttcacgt tatcaaacgg agctttttcg
     8041 gtattgaatg ccacataagc aatgttcaaa cccggttgag aaagtaaatt gattttcgga
     8101 tcatttttca ttttctcaat gtcggttgca tttgggaagt ccattaaatc acattgaccg
     8161 gattgtaatt tcgcataacg ggttgttgca tccggcacga tctcaaaaat caaacggtca
     8221 atgtcaggct tacctttcca atactctttg tttgctacat aacgactggc ttggtctagc
     8281 acataaccgc taaacacaaa cggacctgtg ccgattggcg tggtatctac cgcttcaggt
     8341 atgccggctt tcatcatttt atccgcatat tccgccgaat aaatggaggt gaaatccata
     8401 ccaaggctgg ataagaatga cgcatcacgt ttggttaagg taatacgaac ggtgctgtcg
     8461 tccacttttt ccaccgattt caataatgtc gggaatttca ttgcgttgaa atacggataa
     8521 gtgccgttag agacggtgtg atacggatga tttttatcta actgacggtt aaatgaaaat
     8581 actacatcgt ccgcattcaa ttcacggctt ggagtgaagt ctttgtttga atggaatttc
     8641 acgcctttgc gtaatttaaa ggtgtaagtt aagccgtctt cgctgatttc ccagctttct
     8701 gccaatgccg gttcaatatc ggttgtaccc ggcacaaatt ccaccaagcg gttgtaaatt
     8761 tgttgcgagc tggcgttata agataaaccg tccatcatta aaatcgggct aaaaccggtt
     8821 ggcgaacggc taacgcagtt tacaaaggtt ttctcggttt gcggttttgc tgctgccgca
     8881 gtaggtttat tttcgttgct tttatcatcg caagccgcta gagctaatac cgctaaaacg
     8941 cttgcactaa ttttggttaa cgttggaaat ttcatattat tctccgctta atcttaggat
     9001 taacttacga agattacctt ttccacaatg aggagtaaag aacaaaaagc attatttttg
     9061 caaatcaatc catagaatta gataccgtca acgtcattgc cacccgtgat ccaagtaaat
     9121 ttgccgatac gccacaaaaa caaactaaag atgcactttt ggtaaaacaa gcgacgagtg
     9181 tggcggcggc attaaaagcg ttgccgaatg tggatgtgca aggaggggcc cgtgcgattg
     9241 tgcagaagcc gtctattcgt gggttgagcg ataatcgtat tgtgcaggtg attgacggag
     9301 tgcgtcagaa ttttgatttg gctcaccgtg gttcttattt tgtgccgatg tcattgattc
     9361 aagaaattga agtaatcaaa ggtccaagca gctcgttgtg ggggagcggt gccttaggtg
     9421 gggtggtggc aatgcgcaca ccgaatgcgt tggatttatt gaaaaataat gacaaattcg
     9481 gggcaaaaat ccgtcaaggt tatcaaacgg cgaataatct ttccgaaacc gaagcctcgg
     9541 tgtttgcagc aaatgatcgg tttgatgccc tgctgagtgg tttctacaat aatgctgaca
     9601 atttgcgtag cggtgaaggc aaggaactga ataacacggg ctataagcaa gccggcggtt
     9661 tagtaaaatt cggctggcaa attaacgata ctcatcgttt ggaattatcc catcgtcata
     9721 gccaatttaa acaaactgca ccgggtaata acgaggtaga aaatcaattt accgataatg
     9781 atgttagtgc acaaattagt gcttggcatg cggctaatcc tggaaatcca agtaatccag
     9841 tatatcttgc gaaaatgtta gccttttacg gtcaattaca ttcaagtctt ggtagtgtga
     9901 gttatttaag tgatcaaaaa attaccgata aaagcacctc acttaactac tatttcaacc
     9961 caagcaatcc ttatttgagc acacaagtga cattatatag caatagtacc actgaaaacg
    10021 aacatcgaaa aataagtggt gtggaagatc aaactaaact aagcactcgt ggtgtgaata
    10081 tccgtaatca ttccgaattg tcttggttct ccttggtgta tggcgtggat tatatgttgg
    10141 ataaagtagc cactcagcgt gggagaaata atacggacgc acaataccgt gctaatcctt
    10201 atgatgctaa atctaaaact acaggtgctt atttaattgc acatattccg ctgtttgatg
    10261 aaaaagtggt cttttctccg agcgtgcgtt acgaccgttt cgatacttca agtgaaacgg
    10321 tgaaatataa agacagccat tggtctcctg ctgcaaaact tacttggaaa gcgacaaatt
    10381 ggttggattt aaccgcgaaa tataacgaag ctttcagagc cccttctatg caagaacgat
    10441 ttgtgagcgg gtctcatttt ggtaccacag tgagaggatt acctattaat aatattttct
    10501 taactaaccc taatttacgc ccggaaacgg caaaaaataa ggaagtaacc ttcaatgtac
    10561 attttgataa tgtcatctcg caaggagata aatttaactt tgaagcgact tatttccgta
    10621 atgatgtgaa ggatttgatt aatcttcaag tattcaaagc aaatcctaca agcccaatgg
    10681 aatttattcc cactcgttcc caatatcaaa atattgcgaa tgcacgtttg agtggtattg
    10741 aattacaaac tcgttatcaa actgaacgtt tggcagtttt tgctaactac ggtagtacaa
    10801 aaggtaaaga taaagacagc ggggaagcct tgtcgaacat tgctgcaagc aaaatcggct
    10861 tcggggtcaa ttatgccgtg gtgcaagaca aattcacggt gggggcaaac gtaacccgtt
    10921 atcaagcaca acatcgtgtg ccgaaaaacc acggcgtgac ttaccaaggc tatacgctga
    10981 ccgatctaca cgccacttac gccccattaa aaggcgaatg gaaaaacctg cgtttagacg
    11041 tggcggttga aaacctattc gataaaaaat accaaccggc attcagctta atggaaggca
    11101 gcggtcgtaa tgtgaaactc agtgcggctt acagcttcta atgtctttaa cggaagacgg
    11161 cacaagcggt cgttttccgg ttattttttg caatattaaa agggagttac tatgaaattt
    11221 tatccgtcgc tgatcgcaag tgcggtcagt tttgccgttg ttttgagtgg acaagcggca
    11281 gtgaatgtgc cggatgccgg ctcgttacag cgtgaattag accaacgtca agctcaacaa
    11341 aaagtcaaac cgagtggcga atggtttaat caagcggcaa aagcacctta taccgcaacg
    11401 gacggtcaag aactgacatt tccattaact caggtacaag tttttgatcg aaccaaccaa
    11461 gaaatctcta ccccggaatt agccccgatt ttggcaaact atgtgggtaa agcggtttcg
    11521 ctgaccgatt taagcaactt ggcaaatgag atcaccgagt tttatcgtag ccacaattat
    11581 ctggtggcaa aagccatttt accgccacag gaaattgaac aaggtaaggt gaaaatcctg
    11641 ttgtttaaag gcaatgtcgg tgaagtgcgg ttacaaaatc agagtgcttt aagcgataaa
    11701 tttgttgcac gccttgccaa caccacagtg aatggttcgg aatttatcct aaaagacgaa
    11761 ttggaaaaat ttgccctcac gatgaatgat gttcccgggg tgaataccgg cttggagctt
    11821 tctgccggta agcaagcagg cgaggcgaat ttgctgatta agctccaaaa tgccgagcgt
    11881 ttctcaggct atgtatcggc agataaccaa ggcaataaaa ataccggacg ttaccgttta
    11941 gccacaggga taaaagccaa taatctgata ggttggggcg atgagttgaa agtggatttg
    12001 atgagttcca acaacgctaa tttaaaaaat gcgcgtttgg attattcgag cttgattgac
    12061 ggctacgcta cccgtttggg cttaacggcg aattatctcg actacgaatt aggcggtaac
    12121 tttaaagccc tgcaatccca agggcatact cacaatcttg gggcttatgt gttgcacccg
    12181 acaatccgct tgccggattt tcggttaaac accaaagtca gctttaatca tcaacgttta
    12241 accgacaaac aacaagcggt caatgtggca caaaaacgcc aagtaaacag cttaacggtg
    12301 ggcattaacg ggtcgtggaa ttcgcttaaa gacggcacaa gctatttctc tctgtctgcc
    12361 ttgttcggca atttagctaa ccacaccaac gagcaagcgc attatgtggc aacagatttt
    12421 cagcccaaat cccgttttac ggtctatcaa tacagcctgt ctcacgaaca agcgctgcca
    12481 aaatcctttg ccttcaacct tggcgtgaac gggcagtttg cggataaaac cctcgacagc
    12541 tcacaaaaaa tgttactggg cggtttatac ggcgtgcgtg gtcatcaagc cggtgtcgct
    12601 tcggtggatg aggggcattt agtgcaaacc gaactcaagc attatttacc gctcttttcc
    12661 aaaagcctgc tgaccagctc ggtcttctat gattatggtt ggggaaaata ctataaacac
    12721 agcggatttt tggcgcgtgg tgtaaacaat agcgtgaaat tacagagtat cggagcaggg
    12781 ctttcgctct ctgaagcagg cagttatgcc attaacgcca gtgttgccaa accgctgggt
    12841 aaaaaaatcg agaatgcaga taaacaccaa ttctggttct ccgccatcaa aaccttctaa
    12901 ggagacaaac tatgtttaaa cgtaatgcaa tttctctctg cattttgacc gcttgcagcg
    12961 gtttagccta tgccaataca gcagatttgc cgcaaggtga aagggtgcgt gtcgggacgg
    13021 caacgattga gcgcacaacc gataaaatga ccgtgaatca atccagcaat aaagtacaaa
    13081 ttgattggaa cagctttgat attggtaaga ataaagaagt ggaattcaaa cagccgacag
    13141 agatggcggt ggcatataac cgtgttaccg gcggaaacgc cagtcaaatt caaggcaagc
    13201 tgaccgcaaa cggcaaagtg tatttagcca acccgaacgg tgtactcatc accaaagatg
    13261 cggaagtgaa tgtgggcggt ttgttagtga ccacaaaaga tttagaaaaa atcagcgaaa
    13321 acggcacaaa agataaattc agtcgcaaat tagcgaaaga aggcaaaatt cttaaccaag
    13381 gtaaaattac ggccaaaggc gagcgggctt ttgtggtatt ggtgggcgac gatgtggcta
    13441 atgaagggga aattaacgcc aaaaattatt accacaaaga aacgaaaaaa gtcaccgaat
    13501 gttatgacga ttcttggagc ttggtaaaac aatgctacga ttttgataaa gaaatcgtga
    13561 aaagtaccag tggagaagtg catttatcct ccggagaaaa tgtcaccttt actttaagcg
    13621 accgctatat tgatgtggcg ttagacgata atacggtcaa tagcattatc aaaaatgacg
    13681 gggcaatcat cgccaatggt gacattaccc tcactgccaa agggcgaaat caagccttag
    13741 acagcttaat tatcaataac ggcgtattgc aagccaataa ggtgcaaaat aaaaacggta
    13801 cgatcatttt atctgccggt gaaacccaat tacacgaaaa aagccaaatt aaaggcgaga
    13861 aagtgagctt tgccgaagct tcttattacg gtccgacaga agacattaaa gtggtttcca
    13921 aaaccggctc aaaagtgacc gcacctgtga tcgatttcaa aggtaaaacc gtgaatattc
    13981 aaggggaatt tgggcgtgaa gacagcaccg atctctataa cgatgaacta caaatcctca
    14041 aaaccacggt gaatattgaa gtcccgaata cggaagatat tcgcattgca gataccgaca
    14101 acggaggtag cggttccttt atacaagcgg gtgctttagc ctcattactt gcaaataacg
    14161 gacgggttaa tgtacagggc aaaggttttg atgtctcagg caagttgaat gtgaatgcct
    14221 ttaaacaaac ggattccgtg ctgaaattta tcaatcaggc gggcatcaat atccacaatg
    14281 ccgatattca cgcaaacggg cgtttattct ttgtgacaaa cttgccgaat gacaccgatt
    14341 tccaatcgaa tatccgcatt accgacagca aaatcaactt aggcaacggc tcaatgggct
    14401 ttggtcgcag tagcaaacgt tgggaaatgc acgatgtggt tcgacgtgcc gatacggaac
    14461 tgcgtaaaaa attcaatctg gatatgaaaa acgtggaatt aaaccaagtg gatgattttg
    14521 tggtagcggg cggtttccaa caagttaatt tggataacgt gaaagcccgt ggcaaaacca
    14581 acttctatct ggattccggc aactcccgca tgtttgtcgg tgagcagatt tatgaatatg
    14641 gcgtaaccga tattgaaaaa cgcacccaat tggcgaattt agaccagcgt agaaaacgtt
    14701 ggaattcgga taactatgat gagaccgatc tcaactggga atacaaccaa cgctttgacg
    14761 gctttgcagc aaaacacaat caccgcgcgc cgattgccga tacggaaatc aatattgcca
    14821 atagtgatat cagcttagat aacggttttg tgcatttaat ggcagaaaaa atcaatctgg
    14881 acgacagcaa aatcaatatc accttcaacc aagacaacag cttggatgaa agtacccaaa
    14941 tcaaccgctt aggactaaac ggcaaagtgt cgatgaaaaa cagccatatt aacgtagtgg
    15001 gcgatgaaaa agacggtatc tcaccggctc gccattatgc cacgatgttc ttagtggggg
    15061 agctgcttgg tgaaaagagt tctattttcc tcaaatccca ccaaggtgca gtattgagaa
    15121 ccgacagcaa tgttacaatt gccggtaaaa acagcaaaga cgacctcaaa attaccgcca
    15181 ttaccaccgg taaaaaaatg ggcaatgaaa tcatcggacc ggacggcttg ccaacctcgg
    15241 aagataacga tgccaacgcc agcaacaccg cctttgccat cggctatgaa cctaacgcta
    15301 cggctatcgt cgaaaatgca agcattaccg cattagcacc aaacggcggc ttggcataca
    15361 ccggcacgtc taaaacccaa attgacgcga aggataatgc tacctttacc ttctttgaaa
    15421 aacctcgagc cggctatgac aaattgcttg gcgacatcaa aggtaacccc gataaattaa
    15481 ccccacgcga tttcgcccgt ttatacgaca acattaaagg catcaaagcc agcagcctat
    15541 ccgaacaaca gcttggccta gcagacaccg cgaagaaaac cagcgaacac accttagcaa
    15601 acagcctaga cgtagaaaat ttggtttcag tgacagtgtg tgatgagcag aataagtgtg
    15661 aggagcgtat gttaggtaac aaaaatacca atgctaaagt tattgtcgga gatatcaaat
    15721 ttgagtagcg ttgaagaaag tggtagaagc tcagacttga tctgataaag cactataaaa
    15781 agtgagagtt tcccgtttag aatatgagtg tcaaaattca atctaaacaa aaaggaaact
    15841 ctcatgtttt attacttaca atcccctcat taaacacaag accggtttat taaatttagc
    15901 agaagaaccg ggcaatatct ctcaagcctg caatgtggtg ggtatgagcc tagatacatt
    15961 ctattgctat caacaagcgg ttatttttgg caactaattt gcaaaaaaac aataaaatcc
    16021 gaccgcttgt agagggttaa tccgccctta gtgaaatttc accgatgccg aaatagcgga
    16081 ggttgtcgcc ctgttttaga atgagttcgc cctgctcgtt aatgccttgc gaaatgccgt
    16141 ggatttcttc ggcttcggta atgagcttaa ccgctttatg gcggaaaagg tcgaaacttt
    16201 gccagcgttc ggcataatga gcgaagccga caagcggata aattttgaga tttttttgca
    16261 attcataggc taaacggcaa accaattcgt tgcggttgaa atgatattgc gataaatctg
    16321 cccacgcttg cgtcacaata ttttcatcca cttttgccat tcctaggttc agcccaatgc
    16381 cgataaccac atctaaacca ttgcgattgg cttgggtttc caacaaaatg ccccccattt
    16441 ttttgccttg gtaataaatg tcattcggcc atttaatttg aatatctttt acattttgaa
    16501 cagtaaggct ttccgcaatg atcaaggcta ccactaaact taagggcggt aaatctgccg
    16561 cctgctccat cgggtaatgc cataaaatcg agaaatagag attttcactc tcgggcgaat
    16621 accaagttcg acctcgtctg ccacgtccgg cagtttgttt ttcagcgagg caaaccgtgc
    16681 cgttttccaa acgttggtga tgagtgagca aatattcatt ggttgaatca atttcatcga
    16741 acacaacggc ttgcccgcaa attaaagcat tttggatttg agcttggtta agtttcatta
    16801 tttccccact tagcgagtga ttttttcgct atcgacctca ccgtttttac cgataaaacg
    16861 cacttcaggc tgaatttcta ccccaaattt ttctttcact ctctggcgaa cggttttggc
    16921 tagagccaag acatcttgcc ctgttgcatt ttctttatta atcagcacta aggcttgctg
    16981 ggtatgtacc gccgctccgc caatttgtaa gtctttcagc ccggtttggt caattaacca
    17041 acctgcggct aattttaccg ttccatcagc ttgagggtag tgaggaatgg ctgggaattt
    17101 ggcttgtatt ggtgcaaatt gttcgtttga aatcaccgga tttttaaaaa agctgccggc
    17161 attgccaaat tcgtcagggt ttggcaattt ggaggagcga accgcacaga cttcatcaaa
    17221 aatctgtatt ggcgtaacgg tttccggatt gagttgggct aatgatccgt aacttagcac
    17281 cggtttccac gctttggcta actttaagcc taccgagata atcgcaaatt catcacggta
    17341 ttggtgttta aacacacttt cacgatagcc aaattcgcac tcggatttgg atagcttaaa
    17401 gagttctccg gaacgtaaat ttaacacttc cacgaaatca catacctgtt caaattcaac
    17461 cccataagct cctatatttt gaatgggagc agagctggcg acacccggaa ttaacgccag
    17521 attttcaatg ccggcaatat tgtgtgccaa cgtccattta accaattcgt gccagttttc
    17581 cccaccttga acgtgcaaat agtggaaatg ttcgtcttcg tggtgcgtaa tccctttgag
    17641 tttatttact aacaccacac cgtcaaaatc ctctaaaaac aaaacgttag agccttgccc
    17701 gagaatcaag attggcagtt gagcgttaaa ggctttttgc cattcgctca atagctgttc
    17761 gactgtggta aattcaataa tttgggtcgc ttttgcaggc aaatggaaag tgtgaaaagg
    17821 ggttaagcta tgtttcatca tcaaattagt tagttaagaa gtggaaataa caagcggtga
    17881 tatttttaca aaattttgca attaagattt tgggcaggca caggatctgc ccctacattc
    17941 agaattacct tgccccatat accacaatgg ttttgccgtg agcagaaatt aagccttcgt
    18001 cttccaacat ttttataatg cgtccaacgg tttcacggga acaaccgacc atttgcccga
    18061 tttcttggcg ggtaattttg atctgcatac cttccgggtg agtcattgca tccggcattt
    18121 tagctaagtt gagcaaggtt tgagcaatgc gaccggcaac gtctaaaaag gcaagattac
    18181 cgacttggcg tgaagtttga cgtaaacggc gagccatttg tgccgataaa tacattaaaa
    18241 tatcggggtt taaatggact agttgtttga attttttata ggaaatttcc gcaatttcac
    18301 aaggaccttt agtgcggacg taagcggtac gttgttggac tttttcttca aataaactta
    18361 attcaccgac aaactcacct gtgcctaagt tagttaagag catctctttg gtttcatcat
    18421 ctttgatgta aaccgacact gagccattca cgatgtagaa aagtgattct gcatcttcac
    18481 cggcgtgaat gagtgtatat ttcgccggat aacgatggat atgacaatgg gttaaaaacc
    18541 actccaccgc agggtcgtga atagagggcg gaggagttac attttccaga ggagctgttg
    18601 aaagtgtcat atcttgcata gtatttccta taactcttat actaggttaa atttttagaa
    18661 ttttaacacg aaattaaagg ttagcctaaa aaagattttt tgtctttaat atcaaggctt
    18721 tcgtgtaatt ccgaccaaac aatcaccact ttatctgcct taagttgtgt gagtaatcgt
    18781 tctctttttt ctgccagaga aagttccctt tccccgtagt ctgtgccttc tcgtaaaatg
    18841 aaggaatcta aaatattatg taaggttgat tcttcgagtt cttgccaagg tataatcata
    18901 attaatcaaa taaaggctta ataaatgccc actgtttttc aaaatgttgg tgttggttat
    18961 agctgaaatt agaacgaacc aagcgtaaaa attgcccttc gcagaaattc actaaataac
    19021 cggctaaggc tctttcgtct tcaaagctct tgcgttcacg cagtttacta agttgcaaaa
    19081 tattggcaaa ttgtaactcc aagttatcaa agaattttgc cactcgagct ttgagcaact
    19141 cctcttcaaa catcaaggcg tgtccggtca ggatgcgggt aacgcccggg tttttgcgag
    19201 caaattctaa aatcacatat aaaattgctc gcaccgaatt ggcactgctg tcttggcgtt
    19261 tattggcgtg aatatagctg gtgagggtct gctcgatttt ctcgattaac gcttcaaaca
    19321 tcttggtttt acttgggaaa tagcgataca acgccccctc cgacaccccc accgcagcgg
    19381 ctaaacgctc ggtagtgata cgctgcattc cctcttcaga atttaacaag ctgattaaca
    19441 cttccaacac ttgttggcgg cgttccacca ctgatttttt cggcatttta attttaggtt
    19501 ctgtcattgc caatcctatt attgcttacc tgaatgccca aaaccgcctt cgccacggtc
    19561 ggtctgctca aactcttgca caatgttaaa acttgcctga accaccggca caaagactaa
    19621 ctgagcaata cggtcgccca cgttcacggt aaaatcagct tggctacggt tccaaagcga
    19681 cactttcagc tcgccttggt aatcgctatc gatcaagccg actaagttgc ctagcacgat
    19741 cccgttttta tgccctaaac ctgatcttgg taagatcacc gctgccaaat tcggatcggc
    19801 gatataaatt gaaatccccg ttgggatcaa caccgtttca cccgctttta ccgtcagcgg
    19861 ctcgttgagc atcgcccgta gatccaagcc tgccgagcct tcggtcgcat acgcaggtaa
    19921 tggaaattca ttgccgatac ggctatctaa aatttttaaa tcaatttgtt tcattctttt
    19981 tcctttttgt aa
//
LOCUS       DANLXQ010000029        19914 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.029, whole genome shotgun sequence.
ACCESSION   DANLXQ010000029 DANLXQ010000000
VERSION     DANLXQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19914
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.029"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(87..725)
                     /locus_tag="PXD94_000804"
     CDS             complement(87..725)
                     /locus_tag="PXD94_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL6285691.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVQANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            complement(813..1760)
                     /locus_tag="PXD94_000805"
     CDS             complement(813..1760)
                     /locus_tag="PXD94_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL6285692.1"
                     /translation="MKKLAILGECMIELNGEPFGEMHQTYGGDSLNTATYLARVSSPE
                     QIEVRYISALGTDKLSQGMLAAWQVDNINTDFVLRDEHRQPGLYLIQLDKQGERTFLY
                     WRNQSAARYLLQHPGYPKALSALNEVDMVYLSGISLAILPENDRTLLIAQLRELKTQG
                     VKIAFDSNYRPKLWESVAAAQAHYAELLQLVDLALVTFDDEQLLWGDVNEQATFDRLT
                     QYGIQTIVVKQGSLGATVLQNGERTFVPTVAVEKVVDTTSAGDSFNAGFLNGYLLGKD
                     VVTCCQQGNAVAGIVIQHKGAIIAKSATTHLKSQFEQSI"
     gene            complement(1777..2622)
                     /locus_tag="PXD94_000806"
     CDS             complement(1777..2622)
                     /locus_tag="PXD94_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6285693.1"
                     /translation="MSIAQNHNFKDKVVVITGAGGVLCAYFAKEIAKTGAKVALLDIN
                     LEAAQNFASEIADEGYIAKAYQTNVLELESIKQARDEILKDFGTCDILINGAGGNNPK
                     ATTDNEFHDINLPENTKSFFELDKAGIEFVFNLNYLGTLLPTQVFAKDMLGKEGANII
                     NISSMNAFTPLTKIPAYSGAKSAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNKAL
                     LFNADGTPTARANKILTNTPMGRFGQADELVGAILFLMDHHYSSFINGVVLPVDGGFS
                     AYSGV"
     gene            complement(2634..3905)
                     /locus_tag="PXD94_000807"
     CDS             complement(2634..3905)
                     /locus_tag="PXD94_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL6285694.1"
                     /translation="MLWLFLGSLFLFLFLGVPVALSMLLSASFMLWKMDMFDAQLITE
                     NFVMGTNNFPLMAIPFFMLTGEIMKHGGISERIINFAMSMVGHIKGGLGYVVIISGLI
                     FAGLSGSAVADTAALGAILIPMMVARKYDVARSTGLICAAGIISVVIPPSIPMIVYGI
                     TAGASITKLFMGGMVPGFLMVLGLWLVWKFLYRNEEQTVEPKKTGKERWAAFKSAFLP
                     LLLPIIIIVGLRGGIFTPTEAGVVAAIYAAIISIILKGLTLDKIQDVFLGTIKTTAMV
                     MFVAAAAMISAFAVTVAQIPMELVEFIKGITDSPTVLMFVIMIFLLLVGCVMDLIPAV
                     LIFVPVLLPLLRAYNIDIAYFGIMMVINLSIGLITPPVGTVLYVGSGIAKIGIARLTK
                     GIYPFLLVYAAILFLLILFPEIIIVPMNWLS"
     gene            complement(3908..4387)
                     /locus_tag="PXD94_000808"
     CDS             complement(3908..4387)
                     /locus_tag="PXD94_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL6285695.1"
                     /translation="MQISTALDRTLKLLAIIALSTMIILVFFNAVLRYFFDSGIAWSE
                     EFARICFVYMIFLGIILVAKERGHLTVDIVVSALPAKVQMYLLFFADILVLVALSFIT
                     YGAYQLMLLTYTQKMPATEISSAFLYFAAVISSISYFAIVFTNLWKHIRLAFIKEEK"
     gene            complement(4448..5431)
                     /locus_tag="PXD94_000809"
     CDS             complement(4448..5431)
                     /locus_tag="PXD94_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL6285696.1"
                     /translation="MKLKTFTKALLISSLMFTGSAFAKTIIKIGHYNSDVHPSNIALQ
                     EYFKKTIEEKTEGRYEIRIFPNNQLGGEDQIVNGLRNGTIEGGITGLLLQNVDPIFGV
                     WEWPYLFKDNAEAKKVLESPIAQEIGEKMEKYGIKLLAYGMNGFRVISSNKKVENFDG
                     FKGLRLRVPLNSLFVDWAKAMHINPQSMPLSEVFTALEQKVIDGQENPYMLIKDSGLY
                     EVQKYIIESNHIFSPGLLQLSLKTWNKMSKEDQTIFLEAAKLYQEKEWELAMKMEQDV
                     KDFFHKNGKEVIVPSEQFKADMLKASETLYQNFYQKYDWAKDVISKIHAAK"
     gene            complement(5460..6485)
                     /locus_tag="PXD94_000810"
     CDS             complement(5460..6485)
                     /locus_tag="PXD94_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL6285697.1"
                     /translation="MEKPLKSVCLVEPNKVGIQTVSYPVKGENDVLIKVEGMGICGSD
                     IGAFRGTNPLVTYPRILGHEIVGTVIESGKGMPNNINVGDRVIIEPYIYCGHCYPCSI
                     GRTNCCESLKVLGVHIDGAMQEVVRHPAHMIIKTPDMPIHELALAEPLTISLHAIHRT
                     KVKAGEHVAIIGAGAIGLMAALVAKAYGATPILIDILDKRLDYAKSIGIPHTINPTKE
                     NDLEAIKSITNGRMAEVVIEASGANVAVQNTLKYTSFAGRIALTGWPKTETPLPTNLI
                     TFKELNIYGARTSKGAFEEALELLASRRIEPKDIISKVITFDEIPHYIEELSANPDDY
                     LKIIAVF"
     gene            6649..7410
                     /locus_tag="PXD94_000811"
     CDS             6649..7410
                     /locus_tag="PXD94_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL6285698.1"
                     /translation="MQNSSRTYTKIGKILKEEIAQGKYRVGDKLPTERDISERFEVSR
                     TIVREAIVMLEVEKLIEVKKGSGVYVIRVPELFPEDFPEEKDNELRDVGPFELLQARQ
                     LLESSIAEFAAQQATKQDIQVLRNILESEKKLLLEKMDDYSADKEFHLALAHMTGNDV
                     LIQLQEQLWRYRFNSAMWAQLHSRILQHDHHHLWIEDHERILNAIQRKNPALARKAMW
                     QHLENVKTKLLELSDVEDPKFDGYLFNVNPVVVGI"
     gene            7440..8624
                     /gene="uxuA"
                     /locus_tag="PXD94_000812"
     CDS             7440..8624
                     /gene="uxuA"
                     /locus_tag="PXD94_000812"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL6285699.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLVWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            8675..9544
                     /locus_tag="PXD94_000813"
     CDS             8675..9544
                     /locus_tag="PXD94_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6285700.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGEGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPILTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            9712..10026
                     /gene="rsfS"
                     /locus_tag="PXD94_000814"
     CDS             9712..10026
                     /gene="rsfS"
                     /locus_tag="PXD94_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL6285701.1"
                     /translation="MEQNLVEFLTKTLDDLKAQDILAIDVKGKSSITDTMILATGTSS
                     RHVASTAERLITEAKQAGFEVFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKL
                     WA"
     gene            10103..10570
                     /gene="rlmH"
                     /locus_tag="PXD94_000815"
     CDS             10103..10570
                     /gene="rlmH"
                     /locus_tag="PXD94_000815"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL6285702.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            10594..12543
                     /gene="mrdA"
                     /locus_tag="PXD94_000816"
     CDS             10594..12543
                     /gene="mrdA"
                     /locus_tag="PXD94_000816"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL6285703.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDDPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            12536..13663
                     /gene="rodA"
                     /locus_tag="PXD94_000817"
     CDS             12536..13663
                     /gene="rodA"
                     /locus_tag="PXD94_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL6285704.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            13755..14414
                     /locus_tag="PXD94_000818"
     CDS             13755..14414
                     /locus_tag="PXD94_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL6285705.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            14428..15606
                     /locus_tag="PXD94_000819"
     CDS             14428..15606
                     /locus_tag="PXD94_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL6285706.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            15774..16073
                     /locus_tag="PXD94_000820"
     CDS             15774..16073
                     /locus_tag="PXD94_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL6285707.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            16135..16788
                     /gene="lipB"
                     /locus_tag="PXD94_000821"
     CDS             16135..16788
                     /gene="lipB"
                     /locus_tag="PXD94_000821"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL6285708.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDGNTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            16803..17810
                     /gene="lipA"
                     /locus_tag="PXD94_000822"
     CDS             16803..17810
                     /gene="lipA"
                     /locus_tag="PXD94_000822"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL6285709.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            17997..19100
                     /locus_tag="PXD94_000823"
     CDS             17997..19100
                     /locus_tag="PXD94_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6285710.1"
                     /translation="MKKLLLNSVFAATLLAFGATANAEIKTITDVLGREVKVDVPAKR
                     VALTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVN
                     SGTFSTEKTIALKPDVLVLPEIQYQALASEISRIEQAGIPVVVDFNKQTIENHTKSVR
                     VFGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFGK
                     NMWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSMG
                     INIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALYP
                     ELFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(19191..>19914)
                     /locus_tag="PXD94_000824"
     CDS             complement(19191..>19914)
                     /locus_tag="PXD94_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6285711.1"
                     /translation="VVADKINLHFEQGKIYTLLGPNGAGKSSLLKTLFGEIAHQGTID
                     FDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDSLNMYISDELLNEAV
                     DIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDEPVSALDMYHQINLL
                     EYVNAYTRANNLITIMVLHDLSLAAQFSDSVMLLSEGKFKHKAELVICWNAILFRDSI
                     MSILRFYMMETARRLSARFANV"
BASE COUNT         5939 a         3836 c         4318 g         5821 t
ORIGIN      
        1 tatgcaatat ttgttatgct ttgtaaataa gaggaaagag atccattttg ggtagaaaat
       61 agctcattgc ttggaacttt aaatggttat ttcaccaatt caaccacttc cttcgtcaat
      121 ttcccaatct cctcccactt ctgttcctta atcagggatt tctccacaaa ccaagagccg
      181 ccgcaggcga ctacgttggg aatggcgagg tagtcgttga tgttttgtgg cgagatgccg
      241 ccggtcggca taatctgtaa gttgccgtaa gggccgagca gggctttaat cattttcacg
      301 ccgccactgg cttcggcagg gaaaaattta actgcttgta tgccaagttc gagggcggct
      361 tcaatcgcca tcgggttgtt cacacccggt gtaacaggca aacctaacac ttggcaatac
      421 ttaacgatag tcggattaaa acccggagtc acaatgcaat ccgccccagc gtttttggca
      481 gttaagactt gctctttggt cagcactgtg cctgctgcaa tcaatacttc cggatagttg
      541 gcttgcacta atttaattgc ctcttcagct gcttccgagc ggaaagtgat ttccaccacc
      601 ggcaaaccgt tttcggaaag ggttttcgcc agcggtaaaa tatcttctgc tttatccaaa
      661 gcaatcacgg gaacaacttt cagttgtctt aatttttcaa tgatttgttc ggtggtgtaa
      721 cgcatttcta gcctcattat gttgtattgc aagcggtcgc ttttgtaaaa aattttgcaa
      781 atttaaccgc ttgtcgttgt gctattgttg gtctaaatgg attgttcaaa ctgcgatttc
      841 aaatgagtag tggcggattt ggcaataatc gcccctttgt gctgaatgac gataccggca
      901 acggcatttc cttgttggca acaggtaacg acatctttac ccaataagta accgttaagg
      961 aagccggcat taaaggaatc gccggcggaa gtggtatcga ccaccttctc taccgcaaca
     1021 gttggcacaa aagtacgctc cccgttttgc aacaccgttg cccccagaga gccttgtttc
     1081 accacaatgg tctgaatgcc atattgagtg agacggtcaa aggtggcttg ctcattcaca
     1141 tcgccccaaa gcagttgctc gtcatcaaag gtcacgagag ctaaatcgac taattgcaac
     1201 agctcggcat agtgagcttg ggcagcagcc acactttccc acagtttcgg ccggtaattg
     1261 ctgtcaaagg cgattttcac cccttgtgtt ttgagctcac gcaactgtgc aatcagcaaa
     1321 gtgcggtcgt tttccggcaa gatggcaagg ctgataccgc tcaagtaaac catatctacc
     1381 tcgtttagtg ccgagagtgc tttgggatag ccggggtgtt gcaacaaata acgggcggca
     1441 gattgatttc gccaatagag gaacgttcgc tcgccctgtt tatccaactg aatcagatac
     1501 agccccggct ggcggtgctc gtcacgcaac acaaaatccg tgttgatatt atccacctgc
     1561 cacgccgcta acattccctg actgagctta tccgtgccga gagcggaaat ataacgcacc
     1621 tcaatctgtt caggcgaact cacccgagcc aaataggtag cggtgttgag cgaatcccca
     1681 ccataggttt ggtgcatctc cccaaacggc tccccgttta actcaatcat acattcaccg
     1741 agaatggcga gttttttcat aggaatacct tctaagttac acgccgctat aagcagagaa
     1801 tccgccatca acaggtaata caacgccatt gataaagctg gaataatggt gatccattaa
     1861 aaataggatt gcacctacca attcatcggc ttgtccgaaa cgtcccatag gggtattcgt
     1921 gaggatttta ttagctctag ctgttggcgt accatcggcg ttaaatagta gagctttatt
     1981 ttgattactc actaaaaatc ccggtgcaat agcattacag cggataccta cttttgagaa
     2041 ataaactgct aaccattgtg taaagttact gattgcagat tttgctccag aataggcagg
     2101 gattttagtt aatggagtaa aggcattcat actggatata ttaatgatat tagcgccctc
     2161 tttacccaac atatcttttg cgaaaacctg tgttggtaat agtgtgccta agtaatttaa
     2221 attaaataca aattcaatcc ctgctttatc taattcaaaa aaagattttg tattttccgg
     2281 taagttaata tcgtgaaatt cattatctgt cgttgctttt ggattattcc cacctgcacc
     2341 attaattaaa atatcacaag tgccaaaatc ttttagaatt tcatctcttg cctgttttat
     2401 gctttctaat tcaagtacat tagtttggta cgctttcgca atatagcctt catcggcaat
     2461 ttcagaagca aaattctgtg ctgcttctag attgatatcg agtagtgcaa cttttgcacc
     2521 tgtttttgca atttcttttg caaaataagc acatagcaca ccacctgctc cggtaataac
     2581 aacaacttta tctttaaaat tatgattttg ggcaatagac ataataagaa ctcctaagat
     2641 aaccaattca ttggtacgat aataatttca gggaaaagta ttaataagaa cagtattgca
     2701 gcatagacta gcaagaatgg atagatacct ttggttaatc tagcaatacc aatttttgct
     2761 ataccagaac caacatataa taccgttcca acaggaggcg taattaaacc gatagatagg
     2821 ttaattacca tcataatacc aaagtaagca atatcgatgt tatacgctct tagtaatggt
     2881 aatagtactg gtacaaaaat taatactgct gggattaagt ccattacaca gccgactaat
     2941 aataagaaaa tcataatgac gaacattagt acagtcgggc tatcggtaat acctttgata
     3001 aactcaacta actccatagg tatttgagca acagtaactg caaatgcaga tatcattgcc
     3061 gccgctgcaa caaacataac cattgcagtt gttttgatag tgcctaagaa aacatcttgg
     3121 attttatcta aggttaaccc ttttaagata atgctgatta ttgctgcata aattgctgct
     3181 acaactcccg cttctgttgg tgtaaagatg ccgcctctta aacctacgat aataataatt
     3241 ggaagtagca atggtaagaa tgctgattta aatgcagccc aacgttcttt acctgttttt
     3301 ttcggttcaa cggtttgttc ttcgtttcgg tataagaatt tccaaactaa ccataaacct
     3361 aaaaccatta aaaagcctgg aaccattcct cccataaata atttggtaat tgatgcaccg
     3421 gctgtaatac catagacaat cattggaatg cttggtggga taactacaga aataatacct
     3481 gctgcacaga ttaagcctgt tgaacgtgca acatcatatt ttcttgcaac catcatcgga
     3541 ataagaattg caccgagagc cgcggtatca gcaacagcgg agccggataa acctgcgaaa
     3601 attaagcctg aaataatcac tacatagccc aatccgcctt taatatggcc gaccatgctc
     3661 attgcaaaat tgataatacg ttcggaaata ccgccgtgtt tcataatttc accggtcagc
     3721 ataaaaaacg ggatagccat cagcggaaaa ttatttgtac ccattacgaa attctccgtg
     3781 attaattggg catcaaacat atccattttc catagcataa aactggcact aagtagcata
     3841 gagagagcaa cgggcactcc taaaaagaga aagaggaata gacttcctaa aaatagccat
     3901 aacatattta tttctcctct ttaatgaaag ctagccgaat atgtttccat aaatttgtga
     3961 atacaatagc gaaataggag atagatgaaa taactgctgc aaaatataga aacgcagatg
     4021 aaatttcagt tgctggcatt ttttgtgtgt atgtcaataa cattaactgg tatgcaccgt
     4081 aagtaataaa tgacagtgca actaagacta aaatatcagc aaagaacagt aagtacattt
     4141 gtacttttgc cggtagtgca gaaacaacaa tatcaacggt gaggtgtccc ctttcctttg
     4201 caacgaggat tattcctaga aagatcatat agacaaagca gattctggcg aattcctcag
     4261 accaagctat tccggagtca aaaaaatatc gtaaaaccgc attaaaaaag actaggataa
     4321 tcattgtaga tagagcaatt atcgcaagga gtttgagagt tctatctaat gctgtagaaa
     4381 tttgcataaa tttctccttc ttcatttaga tgggcgacct cttttatccg gtcgcctcgc
     4441 ttattgctta tttggctgcg tggatttttg aaataacatc tttagcccaa tcatatttct
     4501 gatagaaatt ttgataaagc gtttcggatg cttttaacat atcagcttta aattgttcac
     4561 tcggaacaat gacttctttg ccatttttgt ggaagaaatc ttttacatct tgttccatct
     4621 tcatcgctaa ttcccactct ttttcttggt agagttttgc tgcctctaag aagattgtct
     4681 gatcttcttt agacatttta ttccaagttt tcaaactaag ttgtagtaag ccaggtgaga
     4741 aaatatgatt agattcaata atatattttt gcacctcgta taaaccagaa tctttaatta
     4801 acatatatgg attttcttgc ccatcaatga ctttttgttc tagtgcggta aatacttcac
     4861 ttaaaggcat actttgaggg ttaatatgca ttgcttttgc ccagtccaca aacaacgaat
     4921 ttagtggaac ccttaatcgc aaacctttaa atccatcaaa attctcaact tttttattgg
     4981 aagagataac acggaagcca ttcataccat acgctagtaa tttaatgccg tacttctcca
     5041 ttttttcccc gatttcttga gcaataggag attctagaac tttcttagct tcggcattat
     5101 ctttaaatag atatggccac tcccatacgc caaaaattgg atcaacattt tgtagtaata
     5161 aacctgtaat gccaccttca atcgtgccat tacgcaaacc atttacaatt tgatcttctc
     5221 ctcccaactg gttatttggg aaaatacgga tttcgtagcg tccttctgtt ttttcttcaa
     5281 tggttttttt gaagtattcc tgcaatgcga tatttgatgg atgaacatct gaattatagt
     5341 gaccaatctt aatgatagtc ttcgcaaatg ccgaaccggt gaacatcagg ctagaaatta
     5401 atagagcttt agtgaaagtt ttgagtttca tagttattac ctcgtaggtg agttagtatt
     5461 taaaatacag cgatgatttt taagtaatca tcaggattag ctgagagttc ttcgatatag
     5521 tgaggtattt catcaaatgt aattacctta ctaatgatat cttttggttc aattcttctt
     5581 gatgccagaa gctctaatgc ttcttcaaat gcccccttgc ttgttcttgc cccataaata
     5641 ttaagttctt tgaaggtgat taaattagtt ggtaatggtg tttctgtttt cggccatccg
     5701 gttaaagcaa tacgtccagc aaaagaggtg tatttaagcg tattttgtac tgcgacattt
     5761 gctcctgacg cttcaataac cacctctgcc attcttccat tggtaattga tttaatagct
     5821 tcaagatcgt tttcttttgt tggattaatt gtgtgtggaa taccaataga ttttgcgtaa
     5881 tccaatcgct tatctaaaat gtcaattaaa atcggcgtcg ctccataagc tttggcaacc
     5941 aatgcagcca ttagcccaat tgcccctgct ccaatgatag caacatgttc accagctttg
     6001 acttttgttc tatgaattgc gtgtaaggaa atagttaagg gttctgctaa cgctaattcg
     6061 tgtattggca tatcaggtgt tttgattatc atatgggctg gatgtctaac aacctcttgc
     6121 attgccccat caatatgtac ccccaatact tttagagatt cgcaacaatt tgttctacca
     6181 atggagcagg gatagcaatg tccacaatag atgtaaggtt caatgatgac acgatctcct
     6241 acattaatat tatttggcat acctttacca gactcaatta ctgtgccaac gatttcatgc
     6301 cctaaaattc taggataagt aacaagtgga ttagtgcctc taaatgctcc aatatcagag
     6361 ccacatatcc ccataccttc tactttgatt agcacatcat tttcaccttt aacaggataa
     6421 gaaacggttt gtataccaac tttattaggt tctacaagac atactgattt gagtggtttt
     6481 tccatgattt tgtccttttt tgataaagtt aatcgacatt ttgtagtgtc aatggtattt
     6541 tatgcacaga tactaacacc aaaaaacaag tttggcattc caatttttgt gattttgtga
     6601 acctgatcac aaaaatgaaa ttttggaatt ccaatttcgt gaggatttat gcaaaatagt
     6661 tccagaactt acactaaaat aggaaaaatt ctcaaagagg aaattgctca aggaaaatac
     6721 agagtaggag ataagttacc tactgagaga gatatttctg aaagatttga agtgagtaga
     6781 actatcgtaa gggaggcgat agtgatgcta gaagtagaaa agcttattga ggtaaaaaaa
     6841 ggatcgggcg tgtatgtcat tagagtcccg gagctttttc cagaagattt tccagaagag
     6901 aaagataatg agctaaggga tgttgggcca tttgaactac ttcaagcacg acaattatta
     6961 gaaagcagta ttgctgagtt tgcagcacag caggctacca aacaagatat acaagtactt
     7021 cgtaacatat tagaaagtga gaagaaactt ttgttagaga agatggatga ctacagtgca
     7081 gataaggaat ttcatcttgc attagctcat atgacgggta acgatgttct gattcaatta
     7141 caagaacagc tttggcgata ccgctttaat agtgcgatgt gggctcagct acactccaga
     7201 attttacagc atgatcatca ccacttgtgg atagaggatc atgagcgaat tttaaatgca
     7261 atacagagaa aaaatcctgc attagctcgt aaagcaatgt ggcaacatct agaaaatgtt
     7321 aaaactaaat tgcttgaatt gtctgatgta gaggatccta aatttgatgg atatctattc
     7381 aatgtaaacc ccgttgtagt aggtatctaa aacctaaact acacaatgag gagtttacta
     7441 tggaacaagc atggcgttgg tatggaccaa acgatccggt atctttagat gatgtaagac
     7501 aagcaggggc aacggggatt gtgacagcgt tgcaccatat tcccaacggg caggtgtggt
     7561 cggttgagga aattaataaa cgtaaggcat tattagctga aaaagggctg gtttggtcgg
     7621 tggtagaaag tgtgcctgtt cacgaagaaa tcaaaactca aacaggcaac tatcagcaat
     7681 ggattgataa ctataagcaa accttgcgta atttggcaag ctgtgggatt gatacggtgt
     7741 gctataactt tatgccggtg ctagattgga ctcgtaccga tctggagtat gaaatgcctg
     7801 atggctcaaa agcattgcgt tttgaccaag tggcgtttgc ggcgtttgag ttatatatct
     7861 taaaacgccc gggagcggaa gccacttact catcagcaga acaagccgaa gccaaagcct
     7921 attttgagca gatgagtgac gctgatattg caaaattaac caaaaatatc attgccggtt
     7981 tgccggggtc tgaggaaggc tatacgctcg aagaattcca agcacagctt gaccgctatc
     8041 aagggctctc ggctgaaaaa ttccgtaccc atttagccta tttcttaaat gagattgtgc
     8101 cggttgcaca agaggtgggc attcgtatgg cgattcaccc tgacgatccg cctcgcccga
     8161 ttttaggctt gccacgcatt gtttcaacca tagaagatat gcaatggttt gtggaaactc
     8221 aaccgcttgc agccaatggc ttcacgttct gtaccggttc atacggcgta ttagccgaga
     8281 atgatttggt caaaatggca gagcagtttg cagatcgcat ttattttgct cacttacgag
     8341 ctacccaacg tgaagaaaat ccacaaagtt tccacgaagc ggatcatctt gccggtgatg
     8401 tcgatatgtt tggcgtggtg aaagcattac ttaccgaaga gtatcgccgc aaggcaaatg
     8461 gcgatcaccg attaattcca atgcgtccgg atcacggcca tcaaatgctc gatgatctga
     8521 agaaaaaaac caacccaggc tattctgcga ttggtcgttt aagaggatta gcggagttcc
     8581 gtggattgga gttggcactt aagaaagtct attttaacga ttagccttat ttattataat
     8641 gccctctact gtagagggca ttttatagtt tgatatgttc aaaaaatatt tcggcgaaat
     8701 aatccttttt ctcgttacct ttattgctgc agcggggtgg tttttctcta aacacgcttt
     8761 agttggatta ccgcctattg gctttatggg agggcgtttt atattagcct ctttgattct
     8821 tctgcctttt tcttaccgag agctgtatcg tttagataag gttcaagtta aatattcggt
     8881 gattaccggt cttacttacg gaatttatac tattttttgg gtattggggc tggcttttag
     8941 tacagtgttt ggtgaagggg cttttttggt gagccttgcg atgctgattg ctcctttgct
     9001 ctcttggttg atattcaaac atacgccacc gaagattttt tggttagcct tacccattgc
     9061 attaagtggg ctttactttc tttctgcgga gaaaggcatc tttcattttt ccttaggcag
     9121 tacgattttt ctggtgtcat cagtgtttgg ggcgttatat tttgtgttaa ataatcaata
     9181 cgccaaacaa gtctcattgt ttccattaat tactatccaa ttggcgatag tcggtgcggt
     9241 ctgtagcttg tattcctatt ttatggaaag ttggcatttc ccgattctca ccgaagtgtg
     9301 gctctggttt gccgcaagtg tattgattgc gacaaattta cgttttttaa tgcaaacact
     9361 ggggcaaaaa tattgccata ttggtacttc tgcactcatt atgttacttg agccggtttg
     9421 gacattgtta ttgagcgtga tgttattggg ggaacaactt agtgcgacaa aatggctagg
     9481 ttgcagcttg attttgatag cattgattat ttatcgactc cctattctac gactcaaaaa
     9541 ataggtaaaa caaaagcaaa tatgccatag tattggaagc agttaggcat tttagagtaa
     9601 gtaacaagcg gtcatttttg caaaaaatct tgcaaaaatg accgctttct ttcttttatc
     9661 cgttcaatct atgttaaaat tctacatttt tataagatga gagagagaaa cgtggaacag
     9721 aatttagtcg aatttttaac caaaacctta gatgatttaa aggcacaaga tattttagcc
     9781 attgatgtga aagggaaatc tagcattact gatacgatga ttttagccac cggtacatcg
     9841 agccgtcacg ttgcttcaac ggcagagcgt ttaattacgg aagcaaaaca ggctgggttc
     9901 gaggtatttg gagatgaagg taaagcagtt gccgattgga tcgtggtcga ttttgggaag
     9961 gcaatggttc acttgttgca atccgatagc cgtgagatgt accaattaga gaaactttgg
    10021 gcgtagcagt tcaataagga acgacaagcg gtgatttttg caacattttt tgcaaattaa
    10081 accgcttgta agtgaaagag agatgaaaat tcaattaatt gcggttggca ctaaaatgcc
    10141 ggcgtgggta acgacagggt ttgaagagta tcaacgccgt tttccgaaag agatgccgtt
    10201 tgagttggtg gaaattccgg caggaaaacg gggcaagaat gctgatacta aacgcattct
    10261 tgagcttgaa ggcaaagcaa tgttggcggc agcgggcaag gcaaaaatcg tgactttgga
    10321 tattccgggc aaagcgtgga caaccgagca gttagccact cagttagagg gctggaaaaa
    10381 cgatgggcga gatgtagcgt tattgattgg cggcccggaa ggattatcac cggaatgcaa
    10441 agccgctgcc gagcagagtt ggtcgttgtc gcctttaacc ttacctcacc cactggtgcg
    10501 ggtggtggtg gcggaaagtt tgtatcgggc gtggtcggtt accattaatc acccttatca
    10561 tcgagagtag tgagtcattt tagatagtgt tatatgtttg gtaaattagc aaaattcaca
    10621 gcttcaaacc agcgagaaaa ggtgcgagac gggcaggcag aaagcaatct gtatgcccgc
    10681 cgtgccttag ttgcgtttct gggggtgttg gcgttaagtg gtgttttatt aaccaacttg
    10741 tatcacttgc aagtggtgaa ttatgaggac taccaaaccc gttcaaacgg taaccgcatt
    10801 aaattgttgc ctgtgccacc ggctcgaggc ttaatttatg accgtaacgg taaagtattg
    10861 gcggaaaatt taacctattt cgggctgttt attgtgcctg aaaagacgga aaacattgag
    10921 cagactttaa ccgagttgaa ggatattgtt gggctgacgg acgaagatat cgagaattat
    10981 cataaagaga aaaaacgcag ttctcgctat accccaatta tgctcaaagg caatttgaat
    11041 gaggctcaga tggctcgttt tgcggtgaac caataccgtt ttccaagttt agatgtgcag
    11101 gcgtattata agcggaatta tccttacggc gagcttttaa cccatattat cggttatgtg
    11161 gcgaaaatta acgaaaacga taagaaaaag ctgcaagaag ccgggaaatt cggcaattat
    11221 gccggttcgc acgatatggg gaaattgggg attgaaaaat tctatgaaga tcagctccac
    11281 ggtgtcaccg gttttgaaga ggtagaaatt aataaccgag ggaaagtaat ccgtaaattg
    11341 cgtgaccaag caggggttgc cggtagcagt attcgcttga gtattgatgt ggaattacag
    11401 caatatgtga tgtcgttaat tgctaagcat aaaggggcgg tggtggtgct tgaccctcgt
    11461 gatagcagta ttttagcgat ggtctcaaac ccaagttatg acaataattt atttgtgggc
    11521 ggtatttcag ggcaggatta taaagcatta ttggacgatc caacccgacc actttatagc
    11581 cgtactacac aaggcactta tccaccggct tcaactatta agccatttat ggcggtggca
    11641 ggtttggaag agggaatgat taccccttct accaccattt cagaccccgg ctattggatt
    11701 ttgcccggca ctacacaacg ttatcgagat tggaaacgta gcggacacgg tgcaactaat
    11761 gtgaataaag caattaccga atcgtctgat acctttttct atcaattagc ctataaaatg
    11821 gggattgata aaatgtcgga ttggatgaaa aaattcggtt tcggagtgaa aaccggtatc
    11881 gatatttttg aggaatccgc aggggtaatg ccaacccgtg aatggaaaca aaaacgctat
    11941 aaaaaacctt gggtgcaggg cgatactatt ccggttggga ttgggcaggg ctactggatt
    12001 tcgaccccgt tacagttggc gaaagccacg gcggtgttaa tcaacaacgg gcgggtgaat
    12061 acaccgcatt taatgcttga agtgcaaagc agtaattcgg ttacgccgta tcaagaccca
    12121 ttgttgtatg aagatattaa aggcgtgccg gatgtctatt ggaatttagc aaaacaggga
    12181 atgtataacg taaaccacgc cgcaaacggt acggcacgaa aagtgtttgc cggtgcattt
    12241 tatcattcag cagggaaaac cggtacggct caggtgttta acttaaacgg taaaaattac
    12301 gataaaaatg cgattaaaaa agagttgcac gaccacgctt ggtttatcgg ctatgctcct
    12361 tatgaacaac ctaaaatagt ggtggcatta attttagaaa atgcaggcgg tggtggtagt
    12421 gccgcggctc ctgttgctcg tcagattatg gattatgctt tgagaaaagg cattggtgaa
    12481 cagccggatt cgcaaaattc tgagcaaaat aaaccgcttg tcacagagga acatcatgaa
    12541 tagtggcttt aaaaaacttt tctggaaaat tttttcactc gatctccttt tattatcagg
    12601 attattagct attaccggtt atggtttatt agtactatat agcgcaagcg gtgcaaatga
    12661 gcggatgttt accagtagac tgattcaagt ttcgctaggg cttgggctaa tgttgataat
    12721 ggcaatggta cctcctcgct tttatcaaaa agtttcgcct tatctgtata ttttctgtct
    12781 gatattattg attttggtgg atttggtcgg cgaaaccagt aaaggagcgc aacgttggtt
    12841 gaatttagga tttgttcgct ttcagccttc tgagattgcc aagctcgctg tgccactgat
    12901 ggtagccact tatttaggta agcggccgct tcctccgagt tttaaagata cttttatcgc
    12961 cttagccatt attattgtgc caaccttatt ggtggcaatt cagcccgatt tgggaacatc
    13021 tattttagtg tgttctgccg gtatttttgt tttatttttg gcagggctaa gttggaaatt
    13081 aattggcgca ggtgtggtgt ttttagccgg ttttatcccg ataatgtggt ttttcttaat
    13141 gcacgattat caaaaaaccc gggtgatgac cttaattgat cctaataaag acccgttggg
    13201 tgccggctat catattattc aatccaaaat tgcgataggc tctggtggca ttaatggaaa
    13261 aggctggatg gaaggaacgc aatcccagct tgaattttta cctgaaccgc ataccgattt
    13321 tatttttgca gtgttaagcg aagagcatgg tatgataggt gtgctgattt tattagcgat
    13381 ttatttgttt attattgccc gaggtttagt gattggcaca aaatcggatt ctgctttcgg
    13441 gcgtatatta tcaggtggta cagcccttct attttttgtt tacgtttttg taaatattgg
    13501 tatggtgagt ggtattttac ccgtagtcgg tgtaccatta ccgcttttca gttacggcgg
    13561 aacctcctat gtgacgctga tggcggcatt tggtttgatg atgtcaacct atgttcaccg
    13621 taagcgtgta ggtagtaata atccttatga taaactgaaa taaattaaac ttttgctttt
    13681 ttgcggtatc aaaaagcagt gaaacgatca tcgttacaaa caataacgac atccacatag
    13741 aggaaaaaac ttatatgaga ttaggtaaaa ttgtaaccct gttgttagct ggatttctga
    13801 catttagttc attaaattct ttggctgcga caaagaatac aaaagctaaa tcgagtattt
    13861 tagccaagaa gcaagcaact tctaaagcaa taaaaaaagt aggtatttct aaagttaaga
    13921 aaaaagaagt agtggctaaa gctacaaaaa agaaaacaat ttctactgtt acaactaagc
    13981 cgattaaatc tacaaaaaag ctggctcgca aggttgtcaa gaaaacggta accgctaaga
    14041 aaaaattggt aaaaaagcag cataagccac tggtcaaaaa acatttccag actggtgttg
    14101 ccagctatta tgcaaattac tttaatggtc gccgaaccgc taatggggag actttcaata
    14161 acgcaaaaat gacggctgca catcgaactt taccatttgg tactttaatt gaagtgacga
    14221 atttacgaaa tggtcgttct gtggtggtgc gtgtaaatga tcgcggacct tatgctcacg
    14281 cacgggtctt ggatttatca tcagccgcag cgaaaaaaat cggtatgcac cattcgggga
    14341 cggcaagagt gaaaattgcg attgttgata agtcaaaatt gggatcgact aatcagtttt
    14401 atgtaatgaa ttaatttaag agggaatatg aaaaaaacat tagtgaaagc gatgagcgtg
    14461 gtaggttttg cggtttcaag cattgcctat gcggatttaa acactaactt caatttatcg
    14521 gcaccccaga tcaacgcaca aacctatatt ttgatggatt acaactccgg tgaagtttta
    14581 gtgagcctaa atccggatca acgtcaatat cctgcatcat tgaccaaaat gatgactagc
    14641 tacgtagttg gtgaagccct aaaatcggga aaagtgcaaa acagtgatat ggtgatagtc
    14701 accgaaaatt cgtgggcgca aaaattcccc ggctcttcat taatgttttt agatttaaac
    14761 agcaaagttt cagtaggtga gttgatgaaa gggctgatta tcgtctcggg taatgatgcc
    14821 tctgtggcgc ttgctgaaca tgtttcaggc tcacaagcgg cgttcattaa tgaaatgaac
    14881 aaatatgccc aacagtttgg cttaaagaat actcacttta ccactgttca cggtttagat
    14941 gaccctgagc aatattcttc tgctcgtgat atggcgatta ttggttcgca tattattcgt
    15001 gatcagccgg aagagtataa aatttatgcg gaaaaagagt tccaatataa catcaaaaaa
    15061 ccacaaccaa accgcaacgg attattgtgg gataaaacca ttcaagttga tggtatgaaa
    15121 acagggcata ctgataaagc cggctataac ttagtcgcat cagcaattaa cccgaataca
    15181 cgtttaattt ctgtagtgat gggcgtgcca acttataaag ggcgtgaagt ggaaagtaag
    15241 aaattactgc aatggggctt tgcgaacttt gaaaccatta aatctattcc ggcagctcaa
    15301 tcggtggcag agcaaagtgt ctattacggt gaagtcaata aagtacaatt aggctcggtg
    15361 caagatagct ttgtgactat cccgaaaggt cgctcggcag atttaaaagc ccgttatgag
    15421 ttagagaaga aaaacctcga agctccttta gcaaaagggc aggtagtggg taaaatgatc
    15481 taccaacttg acggcaaaga tgttgcaagc actaacttac aagtcttaca agatgtaccg
    15541 gaagccggta ttttcggcaa agcgtgggat tggattgccc ttactgtgaa gagcttgttc
    15601 gattaatctt gttatttgcc tttttatccc catctaggga gcaatgcaag cggtgtgatt
    15661 ttgcaaaaaa tttgcaaaat ttcaccgctt accgttttag atatgaatgt ataaaaagtg
    15721 tagtggcgaa aaatttttcg cccctcgaag aaagaataga agaaggatca aaaatgtctc
    15781 aacccaaagc gataaactta caagatttac ctcaagccaa attaaaagat ctgctagagt
    15841 ttccgtgcca tttcacgttc aaagtggtgg gagcaaaccg agaagatttg gttgatgatg
    15901 tggtagtggt cacgcaaaaa tatgccaaag gcgattacaa tccacgtcag caaaaaagct
    15961 caaaaggtac ctataattcc gtttcgattg atattgtggc ggaaaatatt gagcaagtag
    16021 aaactctcta cacggaactt gccaaaatta atggcgtaag aatggtatta taatgcgagg
    16081 ggcgaatgac attcgccctc aatttttcaa taaagcgatg ttggaaaata gaaaatgaaa
    16141 ttaataattc gccaactagg cattcagcct tatgaagaaa tttggcacca aatgcaatct
    16201 ttcacagata gccgtgatgg gaataccgat gatgagatct ggctggttca gcacccggct
    16261 gtgtttacgc aaggatcggc aggcaaaccg gagcatttgt taaatccaac caatattccg
    16321 gtggtgcaaa gcgaccgtgg cggtcaaatt acttaccacg gcgaggggca gcaaattatg
    16381 tatgtgctga ttgatatcaa acggctgaaa tccttgggta aagaagtgtc ggttcgagat
    16441 ttagtgactg ccctggagca aaccgtggta caaacgttgg cagaatatgg catagaaagt
    16501 tatccaaaag ccgatgcacc cggggtgtat gtggacggca agaaaatctg ctcacttggt
    16561 ttacgaattc gaaaaggttg ctctttccac ggtttggcat taaatatcaa tatggactta
    16621 acgccttttc aaaacatcaa tccgtgcggt tatgcagggc tggaaatgtg ccaattaagt
    16681 gaactggttc atcgccctga tttgcgttgc gaagaggttg ctccaaaatt agtggctcat
    16741 ttcagcacaa ttttaggcta taataaccaa caaattatta acaattagga taaaacacag
    16801 taatggctac atcgacagct caaacgacgg caactgctac agtgaaaaaa atgagccctt
    16861 tcaaaatgga aaaaggggtg aaatatcgtg atgccgccaa aacctctgtg attcaggtgc
    16921 gtaatatcga cccggatcaa gaactcttaa aaaaaccgga gtggatgaaa atcaaactgc
    16981 cggcaaattc ggcaaaaatt gatagcatta aaaacggtat gcgtcgccac ggcttgcact
    17041 cggtgtgcga ggaagcctct tgcccgaatt tacacgaatg ttttaaccac ggtactgcaa
    17101 cctttatgat tatgggggca atctgtaccc gtcgttgccc gttctgtgat gtggcacacg
    17161 gcaaaccgct tccgcttgac ccggacgaac ctcgcaaagt ggcagaaacg gtgcaagata
    17221 tgaaactcaa atatgtggtg attacctcag tggatcgtga tgatttgccc gaccgtggtg
    17281 cggctcattt ctctgcgacc gtgcgtgaaa tcaaagcctt aaatccgcat tgtaaggttg
    17341 aaattttagt gcctgatttc cgtggacgtg tggagcaagc ggtggcaatt ctcaaagaaa
    17401 atccacctga tgtctttaac cacaacctag aaaatgtgcc acgtctctat cgtgaagttc
    17461 gtccgggagc ggattacaaa tggtcgttgg aattattgaa aatcttcaag caagagttcc
    17521 cgcatattcc gaccaaatcc ggcttaatgg taggcttagg tgaaaccaac gaagaaatct
    17581 tggaagtaat gcaagattta cgagaccacg gcgtgacgat gctcaccatc gggcaatact
    17641 tacaaccaag ccgccaccac ttaaaagtgg aacgttatgt gccaccggca gagtttgata
    17701 tgttccgtga ggaagctgaa aaaatgggct ttgaacacgc agcctgcgga ccgttcgtgc
    17761 gttcttctta ccacgcggat ttacaagcga aaggcgaatt ggtgaaataa aatcgataag
    17821 ggcgaatatt atttgccctt tgtttttgtg gtttgcaaag ttttagcaaa attaaaccgc
    17881 ttgtaatctc ttctaattta ctcttctata gataatttcg ttgttgcgaa ttattttcat
    17941 tgactatttg ttatagaaag ttacaattca actttccatt tctttttgga gttccaatga
    18001 aaaaattact attaaacagc gtatttgctg ccacattgct tgcttttggt gcaactgcca
    18061 atgccgaaat taaaaccatc actgatgtat tagggcgtga ggtcaaagtt gatgtgcctg
    18121 ctaaacgtgt tgcactgact ttctattacc ctgactatat tgcggtaacc ggtgttgaaa
    18181 atttcgataa ggtagtaggg atttcacgag agttttggga aaagttcaac ccaggcagtt
    18241 gggcattatt tgcagaaaaa atgccaaatc taaaaaacat tgccgatctt ggctatgtga
    18301 atagtggtac tttttcaaca gaaaaaacca tcgcattaaa accagatgtg ttagtgctac
    18361 ctgaaattca atatcaagcg ttagccagtg aaatttcacg cattgagcaa gctggcattc
    18421 ctgttgtagt agatttcaac aagcaaacta ttgaaaacca tacaaaaagc gtgagagttt
    18481 tcggtcagct tacaggcact gaagagagag cggagaaaat tgccaaagag tatgaagagg
    18541 gcattgctga tattcagagc tgtattgata atgcaaaagt aagtaagccg aaaatctatg
    18601 ctgaatttgg taataaaggc ccgaaagagc atagttttac ctttggtaaa aatatgtggg
    18661 gagcgattgc cgaaacggtg cgaggcgata acattagtgc cccgtttgta gaaaactggg
    18721 ggcagattaa ccctgagcag gtgttagttt ctaaacctga tgtgattatg atttcaggta
    18781 cggaactagg caacgacact aaccctgaga ttatgtcgat ggggattaat attgaagaag
    18841 ccgatgctca aaaacgctta aaaggcttta ttgagcgtac aggttggaaa gagttacctg
    18901 cggtaaaatc agggcgtgtt tatggtttat atcatacagc ttctcgctca atttcagatt
    18961 tagcgagtgc tcaatttatg gcaaaagcac tttatcctga attgtttaaa gatatcgatc
    19021 ctgaaaaaac gtatttggat ttccacaaca actacttacc aattacgcca aaaggcacat
    19081 tctttatgca actgaaataa aaataacaag cggtcatttt gcagatttat tttgcaaaat
    19141 gaccgcttgt attcgctttt actatttgcg gttcaaacag caatcaatat ttaaacattt
    19201 gcgaagcggg cggataatcg gcgagccgtt tccatcatat aaaatctcaa tattgacatt
    19261 atagagtctc tgaataatat cgcattccaa catatcacaa gctctgcctt gtgcttgaat
    19321 ttcccctcgc ttaatagcat cacactatcc gaaaattgag ccgcaaggct taaatcgtgc
    19381 agcaccataa ttgtaattag gttattcgct cgagtgtagg cattcacata ttcaagtaag
    19441 ttgatttgat gatacatatc caacgcactg acaggctcat ctagcagcag aatgtgtggt
    19501 ttacgtagca atacttgtgc gaacattacc atttgccgtt gcccgccact tagccgcata
    19561 atatcttgat gagctagatg tgaaataccg agtttatcca taatatcaac ggcttcattg
    19621 agtaattcat cgctaatata catattcagg ctatcaagtt gccctaataa aataacctct
    19681 aaggcactga gtgaggcctc tacctgtgtg tcttgtggca tatagccaat ttctttacgc
    19741 caactatgca agtggcttga ggttaattta ttaccgtcaa aatcaattgt gccttgatga
    19801 gcaatttccc caaatagagt tttaaggaga gaagatttac ccgcaccatt tgggccaagt
    19861 aaagtataga tcttgccttg ttcaaaatgt agattgattt tatcagccac cact
//
LOCUS       DANLXQ010000030        19328 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.030, whole genome shotgun sequence.
ACCESSION   DANLXQ010000030 DANLXQ010000000
VERSION     DANLXQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19328
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.030"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            23..973
                     /gene="tal"
                     /locus_tag="PXD94_000825"
     CDS             23..973
                     /gene="tal"
                     /locus_tag="PXD94_000825"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL6285712.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
     gene            complement(1035..1308)
                     /locus_tag="PXD94_000826"
                     /pseudo
     CDS             complement(1035..1308)
                     /locus_tag="PXD94_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
     gene            complement(1339..1803)
                     /locus_tag="PXD94_000827"
     CDS             complement(1339..1803)
                     /locus_tag="PXD94_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL6285713.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            complement(1824..2423)
                     /locus_tag="PXD94_000828"
     CDS             complement(1824..2423)
                     /locus_tag="PXD94_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL6285714.1"
                     /translation="MDKIIAENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYE
                     IDHLAVRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVE
                     LPFPKNKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADNSEIKLKISQPQVE
                     GERLPNPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
     gene            2457..4184
                     /gene="argS"
                     /locus_tag="PXD94_000829"
     CDS             2457..4184
                     /gene="argS"
                     /locus_tag="PXD94_000829"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL6285715.1"
                     /translation="MNIQQILSDKIKQAMVASGADSNVEPLVRQSGKPEFGDYQANGA
                     MGAAKKLGMNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAENTNSSLQA
                     VNFGIKTANPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNNVIRANHVG
                     DWGTQFGMLIAYLEKMENEHASEMELSDLEAFYRAAKEHYDSDEAFAEKARNYVVKLQ
                     SGDKYCLTMWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEIVADLKAKG
                     LAVEDDGALVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYETLKADRAL
                     VFSDSRQAQHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRSGGTVKLKD
                     LLDEAVERADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDYVFDWDNML
                     TFEGNTAPYMQYAYTRIRSIFARANIDENALSGSIQLNEPKERALAVKLLQFEEALNG
                     VAKEGMPHILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAKTLKQGLDL
                     LGIKTVEKM"
     gene            4374..5540
                     /gene="cgtA"
                     /locus_tag="PXD94_000830"
     CDS             4374..5540
                     /gene="cgtA"
                     /locus_tag="PXD94_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL6285716.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFIYERH"
     gene            5625..6242
                     /locus_tag="PXD94_000831"
     CDS             5625..6242
                     /locus_tag="PXD94_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL6285717.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(6289..8247)
                     /locus_tag="PXD94_000832"
     CDS             complement(6289..8247)
                     /locus_tag="PXD94_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL6285718.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(8244..8801)
                     /gene="ccmE"
                     /locus_tag="PXD94_000833"
     CDS             complement(8244..8801)
                     /gene="ccmE"
                     /locus_tag="PXD94_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL6285719.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIEPTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(8987..9184)
                     /gene="ccmD"
                     /locus_tag="PXD94_000834"
     CDS             complement(8987..9184)
                     /gene="ccmD"
                     /locus_tag="PXD94_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL6285720.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(9199..9936)
                     /locus_tag="PXD94_000835"
     CDS             complement(9199..9936)
                     /locus_tag="PXD94_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL6285721.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(10136..10798)
                     /gene="ccmB"
                     /locus_tag="PXD94_000836"
     CDS             complement(10136..10798)
                     /gene="ccmB"
                     /locus_tag="PXD94_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL6285722.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(10795..11436)
                     /gene="ccmA"
                     /locus_tag="PXD94_000837"
     CDS             complement(10795..11436)
                     /gene="ccmA"
                     /locus_tag="PXD94_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL6285723.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLFLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(11580..12290)
                     /gene="hda"
                     /locus_tag="PXD94_000838"
     CDS             complement(11580..12290)
                     /gene="hda"
                     /locus_tag="PXD94_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL6285724.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLGSLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            12560..14161
                     /locus_tag="PXD94_000839"
     CDS             12560..14161
                     /locus_tag="PXD94_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6285725.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     TVRYKAFENYWQGTAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIEDLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            14433..15437
                     /locus_tag="PXD94_000840"
     CDS             14433..15437
                     /locus_tag="PXD94_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6285726.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            15539..16432
                     /locus_tag="PXD94_000841"
     CDS             15539..16432
                     /locus_tag="PXD94_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6285727.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            16443..17432
                     /gene="dppD"
                     /locus_tag="PXD94_000842"
     CDS             16443..17432
                     /gene="dppD"
                     /locus_tag="PXD94_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL6285728.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQIINGRQVKCHTPLNAQGEPIRIAV"
     gene            17560..18549
                     /locus_tag="PXD94_000843"
     CDS             17560..18549
                     /locus_tag="PXD94_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6285729.1"
                     /translation="MKENNSLPPFLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIADEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRIACFVVEENN"
     gene            complement(18783..19328)
                     /locus_tag="PXD94_000844"
     CDS             complement(18783..19328)
                     /locus_tag="PXD94_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL6285730.1"
                     /translation="MSSEQLADIRQQMLKFAQLQLQDIALAEDMVQEAFVNAIKNIDN
                     FQRKAALKTWIFAILKNKIIDYLRKKDRIVLESDLADDDEENHFFDNYGHWKEEYYPS
                     SWQEEAVYSKEFWILFEVCLSNLPAKQARVFMMREYLEATSDEICRVVNITSDNLYTL
                     IYRARLQLQQCLSRKLTERNR"
BASE COUNT         5832 a         4233 c         4005 g         5258 t
ORIGIN      
        1 caatcaaaaa aggaatcaga ctatgagcca acttgacacc ttaagcaaaa tgaccgttat
       61 cgttgctgac actggtgata ttgaagcaat caaactttat aagccaaaag atgcaacaac
      121 aaacccgtct ttaattttaa gtgcatcggc attaccgcaa tatgctccat taattgaaga
      181 agcgattaat tacgcaaaag cacaaagtag cgataaagat caacagttag ttgatgctga
      241 agataaatta gcggtaaata ttggtttaga aatcttaaaa cttgtacctg gacgtatttc
      301 tacagaagtt gatgctcgtt tttcttatga tactgaaaaa actatcgcta aagcacgtaa
      361 attaattgga ttatataacg atgcaggcat cagtaatgat cgcattttaa ttaaaattgc
      421 ctctacttgg caaggtataa aggcagcaga acagttagaa aaagaaggca ttaactgtaa
      481 cttaacgtta ttattctctc aagctcaagc tcgtgcgtgt gcagaagcag gggtttactt
      541 aatctctcca ttcgttggtc gtattttaga ctggtacaaa gctcacgaag gtaaagaata
      601 tgctccagct gaagatccag gtgtaatttc agtaaccaat atatacaatt actacaaaca
      661 acacggttat gacactattg taatgggtgc aagtttccgt aatgtaggag agatcattga
      721 gttagctggt tgcgaccgtt taaccatctc accaccgtta ttaaaagagc tacaagagag
      781 cgatgaaaaa ttagtttgca agctagatta caaaggtgaa atcaagcctc gtccagaaaa
      841 aatgacagaa gcagaattct actgggagca taaccaagag ccaatggcag tagaaaaatt
      901 agctgaaggc attcgtaaat ttgcgattga ccaagagaag ttagaagcaa tgcttgctga
      961 aaaacttcaa tagacaacgc ataaaaaaac cggcattaac gataattaat gccggtttta
     1021 atttctataa atgattattt aaactcataa cgtctaatcg tttttagatc gctatcttca
     1081 tttttaattt catcggcaag atgaacacga gcaccaaccg ctgcatgtgc atcatactca
     1141 cgagttagag tcacagtttc accattatct cgttgaactg taagttcaac aacatcgttt
     1201 gatactgctt tgctagataa atgttgcatt acctttatca gaataaacat ctgcagtaca
     1261 agctgctaac ataagtgaag ctaatacagc taaagaaagt tttttcatta ataatatatc
     1321 ctctaggaaa aactaaaatt aaagcagaga aacattcagg tctgaattag accctacgat
     1381 tctcacacgt ttgccaggca caaaaccgtc ttctttcttt tgaacgacca cgatttcttt
     1441 accatcatct ttgcgaataa ccatttccaa tgaagaaact tgactcgctt tttgttctac
     1501 ttgattacca attaccgcac cggcaactgc ccccactgcg gttgcaatcg cttgccctgt
     1561 accaccgcca atcgcattac cggcaacgcc acctaataca ccaccgccga cagtaccgat
     1621 cacaccttca ctgcgtgctt gaattttcac atcacgcact gaaacaatag tgccataagc
     1681 gatagaacga gcttcttttg cctgctcacc ggaataaacg cttccgctaa acacgtctgt
     1741 atttgcacag ccaactaaag caaaacttga cattaatgcg acagctaaag ctaatttttt
     1801 cattttgttt gttctccgaa acgttaattt tctgacataa ttacactttt tatatcataa
     1861 gggtggagct ttagacaata aggattacaa aaagttacat ttctcaagct gatcgcaatg
     1921 cttggatttg gcaatctctc cccttctacc tgaggttggc tgatttttaa tttaatctca
     1981 gaattatcag caagtctaaa gtgcgacaat gcccttgaca cccattgttc cgaactttcg
     2041 ttgtctaaca tcggaaaaac cagctcaata tgttcccagc cttcttctaa ataaacctta
     2101 tttttcggaa aaggcaactc gacaatcttt acaaattgtc cgcaaaattc aaccgcttgt
     2161 attaatgcaa acagccctat cggtctgccg tttacctcgc tctctttaag taaggttgcc
     2221 ccatcaagca aaaggatctt ccactgttct gcggtttcgg tttggttcat tcttaccgca
     2281 agatgatcga tttcatacgc tgctaaatca atgccggcga tttcagcaat ttgtcggatt
     2341 ttctgttcaa attgcgataa atcgccaaaa caagcggtca ttttctcgaa aaattttgca
     2401 ttttctgcta taattttgtc catttttttg taaatagaat ttaaataggt taaacagtga
     2461 atattcaaca aattttatca gataaaatta aacaggcaat ggtagcaagc ggtgcagaca
     2521 gcaatgtcga gcctttagtc cgccaatcgg gcaagccgga atttggcgat taccaagcca
     2581 atggtgcgat gggtgcagcg aaaaaattgg ggatgaatcc gcgtgaattt gcccaaaaaa
     2641 tcttagagaa tgccgatttg aacggtattg ccgataaatt agaaattgcc ggtccgggct
     2701 ttatcaacat tttcttaaac ccagaatggc tggcggagaa cactaacagt tcattgcaag
     2761 cggtcaattt tggcataaaa actgcaaatc cgcagacgat tgtgatcgac tactcctcgc
     2821 caaacgtagc aaaagaaatg cacgtcgggc atttgcgttc caccattatt ggcgatgcgg
     2881 tagttcgtac ccttgcgttt ttaggcaata atgtgattcg tgccaaccac gttggcgact
     2941 ggggtactca attcggtatg ctgatcgctt accttgaaaa aatggaaaat gagcacgcca
     3001 gcgaaatgga gctaagcgat ttagaagcct tctatcgtgc ggcaaaagag cattatgatt
     3061 cagacgaagc attcgccgaa aaagcccgta actatgtggt gaaattacaa agcggcgata
     3121 aatattgctt aacaatgtgg aaaaaattgg tcgatattac aatgcaccac aatcaagaga
     3181 actacaatcg cttaaatgtt acgctcaccg aaaaagatgt aatgggcgaa agcctctaca
     3241 atccaatgtt gcctgaaatt gtggcggatt taaaagccaa aggcttagcg gtggaagatg
     3301 acggtgcttt agtggttttc ctcgatgaat ttaaaaataa agatggcgac ccaatgggcg
     3361 taatcgtgca gaaaaaagat ggcggtttct tatataccac taccgatatt gcggcagcaa
     3421 aatatcgcta cgagacctta aaagctgacc gagccttagt cttttctgac agccgccaag
     3481 ctcaacatat gcaacaggca tggctcatta cccgcaaagc gggctatgtg ccggacagtt
     3541 tcagccttga acacccgttc ttcggaatga tgctaggcaa agacggcaaa ccgttcaaaa
     3601 cccgtagcgg tggcacagtc aaactcaaag atttattaga tgaagcggta gagcgtgccg
     3661 ataaactgat tgccgaacgt tcaaccgagc taactgcgga agaaaaagcc gccgtggttg
     3721 aagcggttgc gattggttcg gtgaaatatt ctgacttatc gaaaaaccgc accaccgatt
     3781 atgtgttcga ttgggacaat atgctgacct ttgaaggcaa taccgccccg tatatgcagt
     3841 atgcctacac ccgcattcgc tccatctttg cccgtgcgaa cattgacgaa aatgcattga
     3901 gcggttcaat ccaactcaat gagccaaaag agcgtgcatt agcagtgaaa ctattgcaat
     3961 ttgaagaggc attaaacggc gtggcaaaag aaggtatgcc acatatttta tgccaatatt
     4021 tatacgagct tgcaggtagt ttctccagtt tctacgaggc ttgcccgatt ctcaatgccg
     4081 aagaaacaac caaaaacagc cgtttgaaac tcgctgcctt aacggcgaaa accttgaaac
     4141 aagggttgga tttactcggt attaaaactg tagagaagat gtaatacaaa ctctctcccc
     4201 ttgtgggaga gagaccgctt atgagcgttc agcgaaataa gcagagagag gggacaagcg
     4261 gtaattttta ttaaaatttt tacacatttt cccctctccc tgatttttct tctgaacgaa
     4321 gaaaaatctg tccctctccc acaaggggag agggcatata aaaggaaaca aatatgaaat
     4381 ttattgacga agccctgatt cgtgtagaag caggcgatgg cggtaatggc tgtgtgagct
     4441 tccgccgtga aaaatttatc ccgaaaggcg gacctgacgg tggtgatggt ggcgatggtg
     4501 gtgatgtgta tttaattgcc gatgaaaacc taaacaccct tatcgactac cgctttgaaa
     4561 aacgttatgc cgctgggcgt ggcgagaatg gtcgcagtgc cggctgtacc ggacatcgtg
     4621 ggcaagatat taccctacga gtgccggttg gaacccgtgc gattgataac gacacccaag
     4681 aaattattgg cgatttaacc cgccacggaa tgaaaatgct ggtggcaaaa ggtggttatc
     4741 acggtttagg taatacccgc tttaaatcct cagtaaaccg agctccacgc caaaaaacca
     4801 acggtacgcc tggcgaaaaa cgtgatttga aattagagct gttactgctt gccgatgtgg
     4861 gaatgcttgg cttaccaaac gccggtaaat ccacctttat ccgcagtgtc tctgctgcca
     4921 aaccaaaagt ggcggattac ccgttcacga cgctggtgcc aagtttaggg gtagcccgtg
     4981 tgggggccga ccgcagtttt gtggttgccg atattccggg gttaattgaa ggtgcagcag
     5041 atggtgcagg gctggggatt cgtttcctaa aacacttgga acgttgccgt attttgattc
     5101 acttagtcga tattatgccg attgatgaaa gcgatccggc tcataatatt tcggtgattg
     5161 aatccgagct ttaccaatac agcgaaaaac tcgccgaaaa accgatctgg ttggtgttta
     5221 ataaaattga caccatcggc gaagaagaag ctgctgagcg agcaaaagac attgccgagc
     5281 aaatcggctg ggaaggcgat tactacttga tttctgccgc taccggtcaa aacgtgcaag
     5341 ctctcactcg tgatattatg gactttattg aagccaaccc acgcgaagtg gaggaagaaa
     5401 ataaagcacc tgaagaagtc aaattcaaat gggaagacta ccacaacgaa gcaatgcaaa
     5461 atgcggtaga agaagattgg gacgacgatt ggagcgaaga agatgacgaa ggcatagagt
     5521 ttatttacga acgccattag tgcaataata caagcggttg tttttaaacg gagatttgca
     5581 aatttccgga gaaaaacaac cgcttgtagg tttaaggaga gtttatgctt agctcaacat
     5641 cgtgcgattt attcaatatt ccgttcttcc aatttgctca gatgaagaaa tattgccctg
     5701 aggagatccc tcgaattaag gcggaatata aaactcaatg ggaaatttgg aaaaagctac
     5761 atcaggaagt ttctcgtcag cttgggcagc cgtttgctcc accacatatt gagaagtggt
     5821 gcaacggttg gcaagttcga gcccattttt ttgcctatta caagtatgaa tttaatcgaa
     5881 attctgcggc tattctttcc gtgattttaa atcgtcgccg tttgcaagtg agccttgatt
     5941 ggcattgtta tcgagccgat cgctcgcaaa ttaacgtgca tcaatacaat caatggtggg
     6001 aaaatttaga tcgggaaaag tttgctgatt ttgagatttg gcacggaagt gagagtgaat
     6061 atgatgattt tagaaccgtt agtcagatgt cagagcaagc gttcacccta actaacgagg
     6121 aagatttttg gtgtatcggc aaaaatgtgg aaaaggacga tttggataac attgatgtag
     6181 tagaatttat ctaccaaacc attcgggcat tattgccact ttatgagaaa tgtcatcaat
     6241 aaacactaaa aagagaggcg aacatcattt gttcgcccct ttcaaaattt aagctatcac
     6301 ttctttttta ttttcaatgc gtaaacggta gcgtttatcc aacatacaga gtaaaccacc
     6361 gagagccata aacagcccgc caatccaaat ccagcgaatg aacggtttat aatacaaacg
     6421 cactgcccaa gagttatctt ctaatttttc gccaagtgca gcgtataaat ctcgggtaaa
     6481 gccccagtca atcgccgctt cggtcattcc cattcggctc accgtataga aacgtttttc
     6541 agcattaagt gtggcttcat atttaccgtt tcgagtaatt tcaagctcgg cagtgccgcc
     6601 ttgatagttt gccccatcgg tgattttaat acctttaaat tcaaattggt aatctaaaat
     6661 ctcagcttta tcacccacat tcattctgac atctcgttca atgctgaaat tttggctaaa
     6721 tgcaatgccg aacactgtca tcgcaacccc taagtgagcc agcaccattc cccaatgtga
     6781 acgggacagt ttgaaaatgc cacttaagaa cgagtggcga tgggttgcac gttggtgtag
     6841 ctcatacagg ctaagtaaca caataaagac agacatcatc gtgcctaaca ccacactcac
     6901 ggtaatggtg ttacggaata agtatggcaa tccgaatcca agcaatgcca ttaaaatagt
     6961 tgcaataatt accggtttac gaatagcaga aacttggtct ctacgccatt tcactaatgg
     7021 tccaatccct aatacaaatg agaaaggaac cattaaaatt aagaacattt ggtcaaaaaa
     7081 tggtgccccg attgaaatcg tccctaagcc gatttgtttg tgaattaatg gcagtaaggt
     7141 ccctaaaaac accaccgata aaaatgccat taataaaata ttattaatga gtagcatcgt
     7201 ttctcttgaa tagcgttcat agttatctaa gcttttaatt tccgagcctt tgaatgcata
     7261 cagcagtaaa gaaccaccta ctactaacac caagtaagcc aagatgtata aaccacgagt
     7321 tggatctgaa gcaaaggcgt gaactgacac caaaatgcct gaacgcacta agaaagtccc
     7381 cactaaacag agcgagaaag caagaatcgc taataagacc gtccacgctt taaatgtgcc
     7441 tcgtttttca gtcactgcta atgaatgaat cagtgctgtt cctgcgagcc acggcattaa
     7501 tgaggcattc tcgacaggat cccagaacca ccagccaccc caaccaagtt cgtagtatgc
     7561 ccaccaagaa ccaagcacga taccgagcgt taaaaatacc cacgctgcca ttgtccacgg
     7621 acgagaccat cttgcccacg cagtatctaa acgccctgtc atcagtgagg caatcgcaaa
     7681 agcaaaggca acggagaaac ccacatagcc catataaagc aacggtgggt ggaagatcaa
     7741 gcccacatct tgcagcattg ggtttaattc tttgccatca atcggaaaat ttgggaaagt
     7801 tctatcaaac gggttggagg taaaaatcac aaacatcaaa aagccgatgc caatgacccc
     7861 cattagtcct aacactcgag caacggcatc ttccggcatt ttacgggtaa aggttgctac
     7921 cccgacagac caaagcgaca acaaccaaac ccaaagcaat aatgagcctt cgtgagcgcc
     7981 ccacaccgca gataaacgat attgcaacgg taaacggcta ttggagttgt tcaccacata
     8041 ttgcacggta aaatcattca ccgcaaacag ataaaacagc gaaccaaaag agacagtcaa
     8101 tgccaaaaac attgcccaag tcatcgggcg ggcaagagca attaaggtgc tattttgctt
     8161 ttctgctccc caaagcggta aaactgccaa caagatagaa atcgctaggg ctaatgccaa
     8221 cgtataattg cctaattctg caatcatttg ctttcccctt gcacattgtt ggctttttct
     8281 gcttccgcct cggctttcat tttgcggtcg gtttccgatt cacctttcag atcttgttca
     8341 gacacacctg attttttatg ctgttctttt aatttatcgc ctaattccgg tggcatatag
     8401 ttttcatcgt gttttgccag aacttccgta gcttttaaac gggttggttc aatcaacacg
     8461 ccttgtgcca caatgccttg cccttcacgg aataaatccg gcaaaattcc ttcgtattcc
     8521 acggtaatcg aaggcccgat atcattgaga tcaaatacta cttttaaact tttatcatca
     8581 cgctttaccg tatcttccac taccattccg cccacacgga tacgctgacc aacttcaggc
     8641 ttttgtttcg gatcatcatt tttaccgttt acaatttcag acggcgtgta aaacagatcg
     8701 atattttggc ttaacgcata aagtgtcaag cctgctgcga cagaaagccc aagtaatatc
     8761 gaaaccacca cttttaatct tgatttgcgt cttggattca tcgtaatcac ccttcaaaat
     8821 aaatgcaaaa ttgcataaaa actcaaaaat aaattagtgt aagaatagca aaaaaccgca
     8881 aaacattccc gatatttcaa ggaagttttg cgatttctca aatcaaatac ctataaagaa
     8941 gtatgaggct atcgttgcaa aaaataaagt tatttcgacc gcttgtttat tgtgatgttt
     9001 gctttaattt ttcttcacgc acaatctctt tttttgcttg agccaaaatc gctttctgtt
     9061 cacggcgact aagccaaatc agcccaatca ttgcaatgaa actgacacca taagagagcc
     9121 atacataaaa gccgtagcca cccattgcaa aaaaatcgct gattgattca aattgaaatg
     9181 tcattgttct ctcctactct aaactgattt tgccagttgt tttacccaag ggcgattacg
     9241 ttcgtccttt aataaggcaa tgcgataacg ccaaatgcta aaccaagcgg taaaaattaa
     9301 gctaccgaaa atcgccaata gcaacggaat taacatttcc accgccatag acggtttatc
     9361 gaatttagtg atggtcgccc cttgatgcaa ggtgttccac cattccaccg aaaagtgaat
     9421 aatcggcaag ttaatcacgc caacaactga cagcacagcc gccgctttgg ctccggtttg
     9481 tctgtcttgg aatgcggaat aaagagccat cacgccgata taaaggaaaa ataacactaa
     9541 agcggaggtt aaacgagcgt cccacaccca ccaagtcccc cacattggtt tgccccaaat
     9601 tgcaccggtt gcaagtgaaa taaaagcgaa tactaagccg ataggtgcca ttgaaatcat
     9661 cgccaagtta gcttggcgaa tttgccaaac caatgccacc aagcctgcca ccgccattga
     9721 gccatatacg cccatcgacc aaattgctgc cggcacgtga ataaagataa tgcgatagct
     9781 atcgccctgt tggtaatctt tcggggcaaa agccaagccc caaacaaaag cagccgctaa
     9841 cataatcaaa cttagccaac ctaaaaacgg ttggattttc cctagcaaat ggtactgcgt
     9901 ttccgatttt gcataagggt gtagccattt ccacatatat ttctccaaag taaataattc
     9961 ccctcacccg tttacaggag agggacgatt tgagcttcgt tcagaagcga aaatcaggga
    10021 gaggggaaaa agttgcaaat tttcataata attcgaccgc ttcccctctc tctgaaaaac
    10081 acctctgaac gaggtgtttt tcggtctctc tccctcaaga gggagagagg aagttttagt
    10141 tcaaactaat tcttaatgca ccggcaatcg caaacggcga aaaagttaaa gtaaccgcta
    10201 aaattgctcc gataatcgcc agttgtccgc tataacccat atttaaggtg gcggcttcta
    10261 ataccgcagc ggcaaaaatc agtaccggta agaataacgg gaggattaat aaactcagca
    10321 acgtaccgcc cttgcgtaag cccacagtga gtgccacgcc aatcgctcct aagcagctta
    10381 aaatcggggt gccgagtaat aatgtcagca ctaacgccca ccaaacgtgg gtttcaagtg
    10441 aaagcaaaat ggcagcaatc ggtgaaagta aaatcaacgg taagccggtc agcagccagt
    10501 gagcaatcac tttcgctaac gcaacctgcg gcaaggcaag cggcagtaac attaattgct
    10561 cgagcgaacc gtctaaataa tcatcacgga ataaccgctc aaaggaaagc aatgcagaaa
    10621 gcaaagcagc tacccacgca atgccgccgg caattttgcc taataattcg gggttaggtc
    10681 ccattaaaat cgggaatacg gtaatcacca ttaaaaaaaa ccacaacgga tttaaaatct
    10741 ccgagggttt acgaaaggca atggtgagtt cccgctgaat aatagtcaat aaaatcatac
    10801 tttaaattga tctaacgaaa taatacgcac tttgtcgctt tcagcagcct ggtggctggt
    10861 aaaaatcact aatctgcctt gctcgcaatg ctgttcaata tgagcaatca gatccgccac
    10921 acccttttta tcaatagcgg taaagggttc atctaaaatc caaagtttgg cgttagtgag
    10981 ccacaatttt gccaacgcca cacggcgttg ctgaccggct gacaaatgcg aacaaggcaa
    11041 atcttcacgc ccgatgagcg acactttcgt caatgcgtgc caaagggcat catcatcttg
    11101 cggtaactgt tggattttct ggtagaaacg caaattttcc cacggtgtta attccggttt
    11161 tacccccgaa taatgcccta aataaaatag ttcagaataa tagtgatcac gctgtttttg
    11221 aattgcaata tcgttccaca gcacctcacc ttccgcaggt aaaaaaagcc cggctaaaat
    11281 acgtaacagg ctggtttttc caatgccgtt atgcccttca atctgcaccc attcaccact
    11341 ttgcacggta aaactgcacc cttcaaacaa acgggtttcg ccccgttcgc aggcaatctg
    11401 attaagttta agctgatatt gggccgtttg gttcattatt tttcatctgg ttgaacattt
    11461 tagccgaagt gtatcataag cctttgattt aacgtagcaa ataaattact tctttagagg
    11521 tagcaagcgg tcaaattttc aaaaagtttt gcaaattttg ataagaatca aaccgcttgt
    11581 tacagagaga ggatttcctt cacaaaagga atggttaatt tacgctgggc ttggagagac
    11641 gctttatcca acttatccaa tattttagag agttgctcta aatccgttcc cgcttttttt
    11701 aacagaaaat gagcgacctc atcggacaat tccacccctt tttggtgagc atttaactgt
    11761 aaaatttggc atttttgttc atcggttaaa tcgtttagct ggtacacttc gccccaagtt
    11821 aagcgggaac gtaaatccgg caattggata ttgagttggt gtggtgggca atcggcactg
    11881 attaacagca aggttttttg cccttcgtta aacaagcctt gttgttctcg aatttggtta
    11941 aacaggttaa aaatagcaag ttcccattca gcatctcctg caatcgcttg aatatcatct
    12001 aggcaaatca cattcagcag ttcggcatta tctaacacca acggcgagaa ataattggct
    12061 ttagtgagcg gaatataact agaagtttgg ttatttaaaa gaaagtggtt acttactgct
    12121 ttcaacaaat gacttttacc gcagctctta ccgccccaaa tgtaaaaaaa cggctgttga
    12181 acatccgtaa aatttcgttt gagggatcca agcaacactt cgctgttttg gctataaaaa
    12241 ttgtcgaaag tgtcttcatc aatttgatga atgggtagag gtaactgcaa ggctttcctt
    12301 aaatctctaa gtttgatatt tttctaagaa tagcggaaaa gcaggggttt gagaagtaaa
    12361 aatagtgaga aaatatccac tcatttttct aatgaagaga tgacaagcgg ttgaatttcc
    12421 acataatttt gtcaatctga ccgaaaaccc accgttttgt gttaaaaaaa gattgcagtt
    12481 agctcaaaaa attgtatatt caaagctttc tacttaaaaa gtagagtaaa acaactatta
    12541 caaacacaac ggagtgaata tgaaaaacat gactaaatta tcgctaataa cattagcgct
    12601 agccgcttca agtagcgtct ttgctgcacc gaaaaccttt gtttactgct tagaggcttc
    12661 gccttcagca ttaagcccag tgctatcaac agacggtgca agctttgatg tgaacgccct
    12721 cccgatttat aacaaattaa ccgactttga agacggtaca actaatgtta ttccctctct
    12781 tgccgaaaaa tgggacattt cggaagacgg caaaacttac accttccacc tgcgtaaagg
    12841 cgtaaaattc cacgacaata aagaatttaa accaagccgt gagttaaatg cagacgatgt
    12901 agtgttctcc attaaccgtc agtttgatcc aaaccatcca ttccacaaag tttccggcgg
    12961 taactacgaa tactttatcg gtatggatat gcaaaatatt atcgataaag tggaaaaagt
    13021 ggacgatcac accgttaaaa tcagcttaaa agtgccaaat gcgccgttct tagctaactt
    13081 agcgatggat ttcaacgccg tttactcagc ggaatatgca gagcaatcct taaaggccgg
    13141 caagcctgag cgtattgata ccaacccaat cggcagcggc ccgttccagt ttgtggatta
    13201 ccaaaaagat gccaccgttc gttacaaagc ctttgaaaac tactggcaag gcacagcgaa
    13261 aattgaccgc ttagtgtttg caattactcc ggatgcatcg gtacgtttag ccaagttaca
    13321 aaaaggcgag tgccacgcaa tgccttaccc taacccggct gatatcgaag atctgaaaaa
    13381 agatccaaat attgaattaa tgagccaatc tggaatgaat atcggctatt tggcattaaa
    13441 ttcatcgatt aaaccattag ataaccaaaa ggtacgtcaa gcattaaatc acgctgtaaa
    13501 caaacaagcg attgtagatg cggtatatca gggagccggt caagtagcga aaaacccaat
    13561 tccaccaaca atgtggagct ataatgaagc ggttcaagac tatgactaca accctgaaaa
    13621 agccaaagca ttgttaaaag aagccggttt tgaaaatggt tttgatactg aactttgggc
    13681 aatgccggta tcacgtccat ataacccgaa tgctcgccgt atggctgaac ttatccaaga
    13741 agactggaaa aaagtgggcg tgaatgcgaa aatcgtcagc tatgaatggg gcgaatatct
    13801 caaacgctta cgccaaggtg aagcggcaac ctcaatgatt ggctggacag gcgataacgg
    13861 tgacccagat aacttcctca acaccttatt aagctgctca gcggtggaag ccggttcaaa
    13921 ctacgccaaa ttctgccaca aaccgtttga agaagtcgtg cttggtgctg cccaagaaag
    13981 cgacaaagca aaacgtaccg agctttataa acaagctcaa gtgatcttca aagagcaagc
    14041 tccttggatt acgattgctc actccaccgt ttacttcccg gtgcgtaaag aagtaaaagg
    14101 ttatgtgatg agtccgttca gcttacataa cttctacaaa gtagatttag cggataaata
    14161 atccgccaac aagcggtaga atttgcagat ttttttacaa atttgaccgc ttgtcctccc
    14221 ctctttgaac tacgaggggc aggggggaaa tttggcagaa gcgattgccg aagtgagagg
    14281 attgtaaaag aggaaataaa ttttctgtct tattgattat tttcttcgtt aattactact
    14341 gtcaaatctc ccctaccccc tctttgctaa agaggggaat cagatcgaga gatcagacaa
    14401 ctttgatgaa aatccaacct aagagatttt aaatgttttc atttatttta aaacgtgtgc
    14461 taatggtgat ccctaccttt atagcgatta cgttaattac ttttgccctt gtgcatttta
    14521 tccccggtga tccaattgag atccgaatgg gggaacgtgg attatcgcct gaggttcatc
    14581 aacaaatgat ggaacaactc gggctgaacc gaccgttgtt tgaacaatac atcagctaca
    14641 tcaaaggtgt attgcacggt gatcttggta actcatttcg taacaacgaa ccggtgctga
    14701 aggaattttt taccctattc ccagccacgg ttgagttggc attttttgcc ctgctctggt
    14761 cgttgatttt cggcattatt ctcggtgtga ttgctgcggt gaaaaaagaa agttggattt
    14821 cacacactgt aaccacgctt tcactgaccg gctactcaat gccaattttc tggtggggct
    14881 tgattttaat tctttatttc tccaccccgc tcggcttacc ggcttcaggt cgtttaccat
    14941 ctgaatactg gattgaagcc gaaaccggtt ttatgctgat tgatacttgg aattcggacg
    15001 aaccaggggc atttttagcg gcaattaaat ctctgatttt gccgtctatc gtactgggga
    15061 caatccctct tgcagttgtg acccgaatga ctcgctcttc aatgttggaa gtgttgggtg
    15121 aagactatat ccgcaccgcc aaagccaaag gcttaaacac tacccgaatt gtgattgttc
    15181 acgcattaag aaatgccttg attccggtgg taacggtcgt tggcttaatt gtcgggcaac
    15241 tgctttcagg agccgtttta acggaaaaca tcttctcgtg gccgggtatc ggcaaatgga
    15301 ttattgatgc aatcaacagc cgtgattacc ctgtactaca aggctcagtg ctgattattg
    15361 ctactattat tattttagtg aaccttacgg tagatctact ctatggcatt gtcaatccga
    15421 ggattcgaca tagttagaaa aattccttcg atttttcccc tctttagcaa cgagggagtt
    15481 acacaagcgg ttcaaattcc gcaatttttt acaagattat ttttagttgg agttaatgat
    15541 gtcaggaata acggcggtaa atccacccgc acccaaaaca ccgttacagg agttttggta
    15601 ctatttttgt caaaatcggg gggcattaat aggacttacc tttattgcaa tcgtctttgt
    15661 tgcgtgcgta ttcgcacctt gggttgcccc ttttgatcca atcgaacaaa atcgcagtgc
    15721 gttattgctc cctcctgctt ggcttgaagg ggggaattcc gctcatctgc tgggtactga
    15781 cgatattggg cgtgatattc tttcccgtat tatttatggt tcaagattgt ccgtctttat
    15841 cggcttagtc attgtgctac tttcttgcgt attcggcgtg attttaggtc tacttgctgg
    15901 ctactatggt ggcacactcg acatcatcat tatgcgtttt gttgatatta tgctggcgat
    15961 tccaagcctg cttttaacta tcggcgtggt cactatttta ggcccttcat tgataaatgc
    16021 cgctattgcg attgcgattg tctccattcc aagttatgtt cgcttgaccc gtgcttctgt
    16081 attaagcgag aaaaaccgtg attatgttac tgcttctcgt gttgctggtg cgagcgtgct
    16141 tcgcttaatg tttgtggtga ttttacctaa ctgtttagct ccgcttatcg ttcagatgac
    16201 gatggggatc tcaaacgcta ttttagaact tgccgccctc ggcttcttag gcattggtgc
    16261 acagccgcca acaccggagc ttggcacaat gcttgccgaa tctcgtggct ttatgcaatc
    16321 ggcaaactgg ctggtgacca ttccgggtct agcgatttta tcgttagtat tagctttcaa
    16381 cttaatgggt gacggcttgc gtgacgccct cgatccaaaa ctgaaacaat aggagatcta
    16441 aaatggcatt attaacggtt gatcaacttt ccgtccattt tggtgaaact aaaacaccat
    16501 ttaaagcggt ggatcgtatc agttacgaag tcaatgaagg cgaagtatta ggcattgtgg
    16561 gcgaatccgg ttcaggaaaa tcggtaagtt ctctcgctat tatgggctta atcgatttcc
    16621 ccggcagggt tatggctgaa aaattgtatt ttaacggcaa tgatttattg gcattaaaac
    16681 caaaagaaaa acaacagatt gtaggggctg atgtctcaat gatcttccaa gatgcaatga
    16741 ccagcttaaa tccgagctat actgtgggct atcagattat ggaagcccta aaagtgcatc
    16801 aaggtggctc aaaagccagc cgtcgtgagc gagcgattga gttgctgacg atggtcggta
    16861 ttcccgatcc tgaatcccgt ttagaagttt atccgcacca gctttcgggt ggtatgagcc
    16921 agagagtgat gattgcgatg gcgattgcct gtcgcccgaa attattgatt gccgatgagc
    16981 cgactactgc gttagatgtg acgattcaag ctcaaatcat tgatttatta cttgaattac
    17041 aacgcaaaga aaatatggcg ttgattttaa tcacgcacga tttagcgtta gttgccgagt
    17101 ctgcccatcg cattatcgtg atgtatgcag ggcaagtcgt ggaagagggc aaagccgagc
    17161 agatattcaa agcaccactg cacccttaca cccaagcatt gctcaaagcc ttaccggaat
    17221 ttgccgaagg gaaatcccgc ctgcaatcac taccgggcgt agtgccgggc aaatatgacc
    17281 gcccacaagg ttgcttactc aaccctcgct gcccgtatgc aaccgaaaaa tgccgaacca
    17341 ttgaaccggc aatgcagata atcaacggca gacaggtgaa atgccacacg ccactgaatg
    17401 cacagggtga gccgataagg atagctgtat aacgtcataa agagtaggga cgcaatgctt
    17461 cgcgtccgtt acaagcggtg atttttaacc gatttttgca aaataacgat agtggacgca
    17521 aagcattgcg tccctacatt ttccgagaag gaaaacagaa tgaaagaaaa taattctctc
    17581 ccaccattcc tcaaagcagt ggacttaaaa aaatactacc cagttaaaaa gggaatgttc
    17641 gctaaaccga aaacggtaaa agcgctggat ggtgtctctt ttacccttga acgtggcaaa
    17701 acccttgcga ttgttggcga atccggctgt ggaaaatcaa ctttggggcg aatgctcact
    17761 atgattgaaa gcccaaccga aggtgaactg ttctacaacg ggcagaattt cttagtcaat
    17821 gacaaagaaa cggctcaact acgccgtaaa aaaatccaaa ttgtgtttca gaacccgtat
    17881 agctcgctca atccacgcaa aaaagtaggt agcattttag aagaaccatt attgattaac
    17941 accaacttat ccgcaaaaga acgcaaagaa aaagtgttgg aaatgatggc gaaagtcggc
    18001 ttaaaaccgg aattttacga ccgttatccg catatgtttt cgggcggaca acgccagcga
    18061 attgcgattg ctcggggctt aatgttgcag cctgatattg tggttgccga tgaacccgtt
    18121 tccgcattag atgtttcggt tcgggcgcaa gtgttgaatt tgatgatgga tttgcaagaa
    18181 gaaatgggct tgtcttacgt tttcatctcg cacgatttat ccgtagtgga acatattgcc
    18241 gatgaagtga tggtgatgta tttagggcgt tgcgtagaac aaggggatac caaaactatc
    18301 ttcagcaatc cacgtcaccc ttatacacaa gcgttactct ccgcaacccc tcgtttgaac
    18361 ccggagcaac gccgtgagcg gattaaactg actggcgaac taccaagccc gcttaatcca
    18421 ccaaaaggct gtgcctttaa cgcccgttgc agatttgcaa ccgatcgttg ctatgtggag
    18481 cgaccagagc tgaaaaccta tcaagacggt actcgcattg cttgttttgt ggtggaggaa
    18541 aataattagg caacgctcgc tatttaaaag gttaaagttt ttcaacttta acctttattt
    18601 ttcatactta atactcctca tcttttgtag caaaatcttt cattaatttg ctgatatgct
    18661 cacaatggtg tttaaatgac aatacgggca aataatcaaa tgcgttttaa cgccaatctt
    18721 ctcttttaaa gtgagttttc gttcctttga gtcagaaatt aaccttgtta cttgtcggca
    18781 attcatctat ttctctccgt taatttcctt gaaagacatt gttgtaactg cagccttgct
    18841 cgatagatta atgtatataa attatctgat gtaatattta ctactcgaca aatttcatct
    18901 gaggttgcct ctaaatattc cctcatcata aataccctag cttgttttgc aggtaaattg
    18961 cttaagcaaa cttcaaataa tatccaaaat tctttggaat aaacagcttc ttcctgccag
    19021 ctagaaggat aatattcttc tttccaatgc ccataattat caaaaaagtg attctcttcg
    19081 tcatcatcag ctagatcact ttctagtact atacgatctt ttttacgtaa ataatcaata
    19141 attttatttt ttagaatagc aaatatccag gttttcaatg ctgctttacg ctggaaatta
    19201 tcaatatttt ttattgcatt aacgaatgct tcttgaacca tatcttcagc aagagcaata
    19261 tcttgtaatt gtaactgagc aaatttcaac atttgctgac gaatatcggc aagctgttcc
    19321 gatgaaat
//
LOCUS       DANLXQ010000031        19267 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.031, whole genome shotgun sequence.
ACCESSION   DANLXQ010000031 DANLXQ010000000
VERSION     DANLXQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19267
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.031"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            146..394
                     /gene="rpsP"
                     /locus_tag="PXD94_000845"
     CDS             146..394
                     /gene="rpsP"
                     /locus_tag="PXD94_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL6285731.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVVDSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            427..954
                     /gene="rimM"
                     /locus_tag="PXD94_000846"
     CDS             427..954
                     /gene="rimM"
                     /locus_tag="PXD94_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL6285732.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQTESIFDYQPWFLKI
                     KGQWQQVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCSVVNLEGYTMGTVAEMMETGSNDVLVVRNNGKDAFGKQERLIPFLYEQVVKRV
                     DLATKTITVEWDAGF"
     gene            999..1754
                     /gene="trmD"
                     /locus_tag="PXD94_000847"
     CDS             999..1754
                     /gene="trmD"
                     /locus_tag="PXD94_000847"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL6285733.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQVECWNPRDFTFD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAALAVEGGEAKVIYLSPQGRKLDQNG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            1781..2131
                     /gene="rplS"
                     /locus_tag="PXD94_000848"
     CDS             1781..2131
                     /gene="rplS"
                     /locus_tag="PXD94_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL6285734.1"
                     /translation="MSNIIKQLEQEQLKQDLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
     gene            2287..2643
                     /locus_tag="PXD94_000849"
     CDS             2287..2643
                     /locus_tag="PXD94_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285735.1"
                     /translation="MKQLSIRLPDDEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSA
                     KNKISEESNIHQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEK
                     MAEMMGYTKEDWESMK"
     gene            2645..2968
                     /locus_tag="PXD94_000850"
     CDS             2645..2968
                     /locus_tag="PXD94_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285736.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(2978..3310)
                     /locus_tag="PXD94_000851"
     CDS             complement(2978..3310)
                     /locus_tag="PXD94_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL6285737.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(3403..4599)
                     /locus_tag="PXD94_000852"
     CDS             complement(3403..4599)
                     /locus_tag="PXD94_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL6285738.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(4748..6091)
                     /gene="accC"
                     /locus_tag="PXD94_000853"
     CDS             complement(4748..6091)
                     /gene="accC"
                     /locus_tag="PXD94_000853"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL6285739.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAQGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPGGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(6163..6630)
                     /locus_tag="PXD94_000854"
     CDS             complement(6163..6630)
                     /locus_tag="PXD94_000854"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL6285740.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTVPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDTLCIVEAMKMMNRIESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(6883..7665)
                     /locus_tag="PXD94_000855"
     CDS             complement(6883..7665)
                     /locus_tag="PXD94_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL6285741.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFNRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(7675..8112)
                     /gene="aroQ"
                     /locus_tag="PXD94_000856"
     CDS             complement(7675..8112)
                     /gene="aroQ"
                     /locus_tag="PXD94_000856"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL6285742.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(8198..8566)
                     /locus_tag="PXD94_000857"
     CDS             complement(8198..8566)
                     /locus_tag="PXD94_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6285743.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKSLE"
     gene            complement(8626..9450)
                     /gene="dapD"
                     /locus_tag="PXD94_000858"
     CDS             complement(8626..9450)
                     /gene="dapD"
                     /locus_tag="PXD94_000858"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL6285744.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVDAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            9598..10260
                     /locus_tag="PXD94_000859"
     CDS             9598..10260
                     /locus_tag="PXD94_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL6285745.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            10257..11009
                     /locus_tag="PXD94_000860"
     CDS             10257..11009
                     /locus_tag="PXD94_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6285746.1"
                     /translation="MIQLINETQQHDKFRQICDKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
     gene            11100..11936
                     /gene="ftsN"
                     /locus_tag="PXD94_000861"
     CDS             11100..11936
                     /gene="ftsN"
                     /locus_tag="PXD94_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL6285747.1"
                     /translation="MPQRDYVARSNEKKSKKNQKESNVKKILMRIIGVILFFGIGLWF
                     LKSSAPVPTVPSAPQPANSQTKSELPSRPEETWSYIRDLETRTVITDNSQTSQERLAQ
                     LTAEQKQQIEERRKQEEARLAAEQAQQKPATDVSATELAPKTEEVKSLTEEELAAKKA
                     QEQALAQKQAEEKRRQAELKKQQEAKKEQPAKIVASAATLADKAANQAGKFGLQCGAF
                     KNKAQAENMQARLAMAGFNARIASSADWNRVFVGPIGDRAATSRAQSNARSVAECVIV
                     AM"
     gene            complement(11978..13159)
                     /locus_tag="PXD94_000862"
     CDS             complement(11978..13159)
                     /locus_tag="PXD94_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL6285748.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     KLGDMVENNVIQIALWEQLKQYPNCLQAVGFEQIFANKAGEYWEVTVDGNTFAAPLLI
                     AADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPAPSGTWQQFYPTGPRAFLPLSG
                     NHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRLNAELGNIQVQASGAFPLTRQH
                     AQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTLLEVIEQAVKKGKNFASEQVLQ
                     RYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNVGLVLADKVSPIKKQALKYAIG
                     I"
     gene            13266..13604
                     /locus_tag="PXD94_000863"
     CDS             13266..13604
                     /locus_tag="PXD94_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285749.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHNKHGDH
                     YDEHVLEVNETNPADCNAAHCCGDHVHGEVCGHEAVPHGDHIDYIVNGRLHHQHGDHC
                     DDHGPVEIVK"
     gene            13736..14605
                     /locus_tag="PXD94_000864"
     CDS             13736..14605
                     /locus_tag="PXD94_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL6285750.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGCEN"
     gene            14697..16508
                     /locus_tag="PXD94_000865"
     CDS             14697..16508
                     /locus_tag="PXD94_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285751.1"
                     /translation="MFYLNNDNFKREPYRGYCPSHLEKLFTKPHLDIRGNGYYYPDNE
                     YVVAWYESDFIFKSRKAQAHAKVKLVNLTCPKYTGKNSAEYLSVLYISVPLNYLHYFT
                     IGSIWKGGVAKEQFALEEFYITVEAENQDGKRENLSIVSFGESEENGLEKPFDYTIYT
                     IPTEYHNYGDDLNRLLSISYQNQKFIIHPLHIFMMHYGYSTDIKRILATYPLDEVRER
                     LFIDKVVENFDVERYVVLPKYFVKKDAIFLYHFKYDEETTGTRVKKLVSQFRLNIHNQ
                     THPIEIDFWHTQKVELKIRGVRLGNAVLCAEIIGLNQPEGEDITLVLSQSKREKSGNK
                     TEEQNNEVNNVPITRVYTREPELDELPLTDNSPDNRTVEYNKRQFELLGRQRQIRALK
                     KAREESNQKMKALTPDEIDSLGVGESDGRNGKVGLAFCFLDDTPVGKSSKLYKLWQHA
                     KAVAEWNHGEAHWYTPNLGFRNDDELLPVSLETNKCFDYPEIAIIIRLQIYGETFFLI
                     DFSMKQRDISIRGLGYKPDPNEDFLYESDMSKSELSELLSAVHHHEHLPKEYIEKKNK
                     EKTKLTTFNHSEAESSNWAYNAVQKLTSRAITKPTYF"
     gene            16517..>19267
                     /locus_tag="PXD94_000866"
     CDS             16517..>19267
                     /locus_tag="PXD94_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDL6285752.1"
                     /translation="MNAEQRVIVTPTFWGKFFGKIKSVELQQNKVIVTDKKNNITEHD
                     LGKTFDFPAIQKSFFGTKLFFKDDSTEVVLSKLAKKQTDSLLLEIEKVVASNIKVKVK
                     EGFQHFANLAENQYLRDSDIPTLNDRVRLSVLSYGDNKEHFQKYFDESLVKKIQYISS
                     LFPVNAINAQTIRREYEQHVLNHRKHFFDVIESNPLTEQQRLSVIRNNDINMVLAAAG
                     TGKTSVMVAKALDLIDSGACKSEDILILAYNRDAATELSERLQERAREANLDLENSPS
                     ISTFHALGRRIIKECGKSVHLSKFAEDPMKLDRWFNKWLEDYIKEDPDNLAKFIQLAY
                     QPIDPFQFKTKDEYDRYVNDNDYRTLQGERVRGYQELLIANWLFSHSVEYEYEPRYVS
                     KRRIEIGFDYKPDFSLGDGVYLEHFGIDRQGRTRADINAQEYNANIQRKRELHAEHNT
                     TLLETYHYNWVENTLYKRLEQLMNEQFIPLKPKSQQEILDALNESGIFKENKNRYLKC
                     LQAIRTERLDYQQILKRLTDAKIVYAKEYATLLMRIHDAYVKELRSANEIDFDDMILL
                     ATQLVKTGEFKPKWKHILVDEFQDISMARLELLKEIYTKGPRPIWTVVGDDWQSIYRF
                     SGGKLEVTTRFNDMVGSHTLSKLEKTYRYNNSIADTAGQFIMQNPEQYQKNVVTHTKV
                     ADSCVHLYDSHVVKDEKSEANISLKASAILKLIRQKAPEATVAILARYRYLLEDAKSV
                     IKDKNVKFWTFHGSKGLEADYCILLGFFQGKVGFPNQNKEEAVVEALLPMLDSFPHSE
                     ERRLFYVALTRAKKESYLIADANAPSEFINELLSPKYKIDIVSKRFQERYRTIFKCNE
                     CSTGYFVMKQGKFGQFYSCTSGEVCRSSPRICEKCGRPMKLREGKFGQFWGCSGYGIK
                     GDQ"
BASE COUNT         5785 a         3913 c         4113 g         5456 t
ORIGIN      
        1 aaaagagtca tatcaatgaa tctcccttgt ctttccgcaa aaaatctgta taatttgacc
       61 gcttgttttc tcttttgaaa caagttcgtt accctcacct cgaacgaggg tttatttttt
      121 actatcaaca tttagaggaa aggttatggt aaccattcgt ttaactcgtg gcggagctaa
      181 aaaacgccca ttttaccaaa tcgtggtagt ggacagccgt tcacctcgtg atggtcgctt
      241 tatcgagcgt atcggtttct tcaacccatt agcagcaggt caagctgagc gtttacgctt
      301 agatgtggct aaagtagatg cttgggttgc taaaggtgct gatttatcag accgtgttgc
      361 ttctttagta aaagaagctc gcaaagcagc ttaattttta agccaaaatt aaataagtag
      421 gtgagtatga gcgaacaaaa gatcgaagtt gtcggaaaat taggttcaac ctatggtatc
      481 cgtggttggt tgcgtctcta ttcatcgaca gaacaaactg agagcatttt cgactaccag
      541 ccgtggtttt taaaaattaa aggtcagtgg cagcaggtcg aattagaaag ctggcgttat
      601 catagcaatg atttgattgt taaattgaaa ggcactgaag accgtgaagc cgcacagtta
      661 ttaaccaacg ctgaaattgg cgtggatttg tctgtgtttc ctgaattgga agagggagat
      721 tactattggc acgatttgat cggctgttcg gtagtaaact tagagggcta cacaatggga
      781 acagttgccg aaatgatgga aacaggttca aatgatgtgc ttgtggtgcg taataacggc
      841 aaagatgctt tcggtaaaca agaacgatta attccgtttt tgtatgaaca agtagttaaa
      901 agagtagatc tcgccaccaa aaccattacg gtggagtggg acgctggttt ctaacctcag
      961 gtatgcggtg agctttggaa aacatttaag gcgttaaaat gtggattggt attatttcac
     1021 tgtttcctga aatgtttaaa gcaattaccg atttcggggt gacaggacgt gcagttaaac
     1081 aaaatctact gcaagttgaa tgttggaatc ctcgtgattt tacattcgat aaacataaaa
     1141 ctgtagatga tcgcccttat ggcggtggtc cgggaatgtt gatgatggtg caacctttgc
     1201 gtgatgcgat tcacgcagcg aaaaaggcgg cattagcagt agaaggcggt gaagctaaag
     1261 tgatttatct ttcgccgcaa ggacgtaaac tcgatcaaaa cggtgtgcaa acactggctt
     1321 caaatcagaa actgatttta atttgtggac gatatgaagg cgtggacgag cgattgattc
     1381 aaacagaagt tgatgaagaa tggtcaatcg gtgattacgt tctcacaggg ggggaattac
     1441 ctgcaatgac attaattgat gcagttgcaa ggtttgttcc cggggtatta ggcaaacagg
     1501 cttccgcgtt agaggattct ttcgcagagg gattattaga ttgcccacat tatacccgcc
     1561 ctgaagtatt agacggtatg cctgttccgg aagtgctgat gtctggacac catgaacaaa
     1621 ttcgtaaatg gcggttggaa caatcgctgg aacgaacgtg gttaagacgc cctgagctat
     1681 tggatagcct agctctgact gacgaacagc gtgtgttgtt agctaaaatt aaaaaacagc
     1741 acaaaatcag ttaattctag gattatagaa ggtttttaaa atgagtaaca tcatcaaaca
     1801 acttgaacaa gaacagttaa aacaagactt acctagcttc cgtccgggtg acacattaga
     1861 agttaaggta tgggtagtag aaggtagtaa aaaacgtttg caagcgttcg aaggcgtggt
     1921 tattgcaatt cgtaaccgtg gcttgcactc tgcattcact ctacgtaaag tttcaaatgg
     1981 cgtaggcgtt gagcgtgtat tccaaactca ctcacctgta attgacagca tttctgttaa
     2041 acgtaaaggt gcggtacgta aagctaaact ttactactta cgtgagcgtt ctggtaaatc
     2101 tgcacgtatc aaagagcgtc ttggcgacta attgctacaa aacagtgaaa tgcacaaaag
     2161 ccttgaggaa actcaaggct tttgcttttt gtacttacaa gcagttattt ttcctcaatg
     2221 atttacaaat cttgctttct tttttgttat aatgttaaca agataacatt aatgataggt
     2281 gtcgatatga aacaattaag tattagattg ccagacgatg aatttacttt tattaaaaca
     2341 tttgcttctg ctcataaact gactttacaa aattttgtga tgtccgcaat ccaagctaaa
     2401 gttgcagaaa tgtctgctaa aaataaaata tctgaagaaa gtaacattca tcaacctctt
     2461 tctaaagatg aaaaaataat gctaaatgag gcaaaagtat tttattctgc tcgttcgcat
     2521 ttaaaggcag aaaatgtgat tcttccgagt gattttatgg agcaaattaa atcaggctgt
     2581 ggagcagaaa aaatggcaga aatgatggga tataccaaag aagattggga gtcaatgaag
     2641 taatatgggg cagattacat ttagtaatat tttcacaaaa gtgatttttg agaatgtgct
     2701 ttctgcttca actaaagaga aaataaaaca aatgcttttt cagtctgtgg ttgcattacc
     2761 tcctctccat tcagaaagaa aaatgatttt acagttgaaa aagcaaaaaa ttaccatttt
     2821 ttatcaagtt attgaggaac aatctgtgca aatcctcgct tatcaatttg gtggaactga
     2881 taaattgaca ggtgtgtcta aagctcactt taaaacacta gatgccattt ttcaaatgac
     2941 ggatgaagag aaagaaggaa aattctaatg taagcggtta tttttccata tttttttgca
     3001 aataacgttt ctcaaatttt gcttgatggc gttcaagcac ttccgtcatc gaaatgccat
     3061 atttatcggc taatacaaaa agattatcca tcacatcgcc taactcttcg attaagttat
     3121 ctcgtttttg ctgttgtgaa gcttcggttt cgtccggtcg ttcacgcccg atttcaatcg
     3181 ctcttactgc acaagccact tcgccgattt cttctgctag ataagtcacc cgcataaaag
     3241 ggcttcgttc ataccaacct tgttgttggt aaaactggcg aacccattgt tgatattgtt
     3301 caatttgcat tttatttctg attcaaaaag tagggacgaa aaatttttcg cccttttata
     3361 agaaaatgta tcagggcaaa tgatgatttg cccctaggag tgttactctg cctcagagag
     3421 aataatccag ccactatcca gccagtcgca aatggtgtct agcattagct ccagctccgc
     3481 ttggtcttgc actttggagg caagtttgtt ccacgaaatc gaatctccat tggcgatacg
     3541 aatcagtaat gccatttctg cttcgtttaa ctcatcaatc cactcgccat ttacataaac
     3601 ccgttgaggg ttctcggtgt agaggatttt tacatttgca tcttgctgta accagccgcc
     3661 ggcttcgaga atttcttgca cttcgtcagg gtaatattca ttgtcagttt gcaataattc
     3721 ataacggcgg gagcttgccg cagtggcaac actgtgagtg agtaactcat cgaactgttc
     3781 ggaattttgg agcaagtcga ttaactgatg tttaaacatt ttcaccattt tcggatcaag
     3841 caacgcattc ggctgttcat tcggcgttaa gcggaacgga agatgaaatt ccgatgaatt
     3901 aatgttgtcg gcttgttgtt cgatggtttt gcagagattt tccagtaaat cggtcgcatt
     3961 tggataacgt aaaccaaaag agaaagttaa accttgctcg ctttcggaaa caccatagtg
     4021 agccattcga gacggcacat aaagcacatc acccgggttc attacctcgt ccattaccag
     4081 ttcgcccata tcatcaaaaa tgcggatcgg ttggtttggt ttgaattcgg tgcttggatc
     4141 gcaccatttg cctaattgcc aacgacgttg cccatagcct tgcaccaaga acacatcgta
     4201 ctcatcataa tgtttgccga cagtgccgtt gagcggtgag caagaaacca taatgtcatc
     4261 acgttgccat tgcgggataa aaccaaaagc ctgccaaagc tcgcctagct caggagacca
     4321 ttgctcgagg ttttgcacca ttacagacca ttgtacaggt aactcttcca aatcttcctc
     4381 aacaagcggg ctggatttaa cagaccattt ctcgttttcg cattgaatca ggcgtgcagt
     4441 gacttcttcc tctaatgcca gttctaaaat atcttccggc tcaaacaatc cgacaatttg
     4501 gggtaagccg ttacgaatta atagcggtcg tttttgccaa tattctcgca aaaaggtttc
     4561 agggctgata ttttcaggta agcagaatgg aatgttcata atggctcctt gctacgtgct
     4621 tctttgagat tactgatatt ctacccgaga aatcaggaaa aacgggaaat tttttatgat
     4681 ttagcgacat cattcacatt ttgaagagga aataatgaaa aaggcgaaca ttgttcgcct
     4741 ttgaaattta ttttaaaccg agttttttct cgagatagtg gatattggtg ccacctttgc
     4801 ggaattcttt atccgcaaga atttgagtat gtaaaggaat attggtttta atgccttcca
     4861 caatggtttc ggaaagggca ttttccatcc ggcgaatcgc aatatcacga ttttcagcaa
     4921 aggtaatcaa tttaccaatc attgaatcgt aatgtggcgg cacgctatag cctgagtaaa
     4981 tatgtgaatc ccaacgcaca cctaaaccac ccggaacgtg taaacgggta attttacccg
     5041 gcgatggcaa gaaggtgttt ggatcttctg cgttgatacg gcactcaatg gcgtgacctt
     5101 tcactttaat atcatcttgt gtgatcgaaa gtggtaagcc ggctgccaca cgcagttgct
     5161 cttttaccaa gtccacaccg gtaatcattt cggttacagg gtgttctacc tgtaagcggg
     5221 tgttcatttc aatgaagtaa aactcgccgt tttcatataa gaactcaaaa gtacctgcac
     5281 cacggtagcc aatttcacgg caggcattgg cacaacgctc cccaataaat tgacgcaatt
     5341 caggtgtaat accctgagca ggtgcttctt ccaccacttt ttggtggcga cgttgcattg
     5401 aacagtcacg ctctgctaaa taaaccgcat taccgtgagt atcggcaagt acttggattt
     5461 caatatggcg tggattttct aaatatttct ccatatacac catatcgtta ttaaatgccg
     5521 ctttcgcttc ggctttcgtc attgcgatag attcttccaa atctttctcg tgatgaacca
     5581 cacgcatacc acgaccgcca ccaccacctg atgctttgat gatcaccggg tagccgatac
     5641 gtttagcgat ttctttattt ttcttcggat cgttaccaac cggaccgtca gaacccggta
     5701 cgcaaggcac gccggctttt ttcattgcat tgatagcaga gactttatcg cccattaagc
     5761 gaatcacatc agccgttgga ccaataaaga taaagcctga tttctcaact tgttcggcaa
     5821 agtttgcgtt ttcagataag aaaccgtagc ccggatgaat agcatcggca ttcgttactt
     5881 cggcagcggc aataatcatc gggatattta aatagctttt taccgatgga gcaggaccga
     5941 tacaaatggt ttcatcggct aatttaacgt gttttaattc acggtcggca gttgaatgca
     6001 ctgcaaccgt tttaatgccc aattctttac aagcacgcaa aatacgcagg gcaatttcac
     6061 cacggtttgc gataacaact ttttctaaca tagggaaatt ccttgtgttc tagaaaatgt
     6121 agggcgtgtt gcacacgccc gttggataat ttaaggaaga gcttattcaa tgatgaataa
     6181 tttttggtcg aattctaccg gttcgccatc attaactaag atttctttta ccacaccggc
     6241 tttgtctgac tcgatacggt tcatcatttt cattgcttct acaatacata acgtatcacc
     6301 gattttcacg gtttgaccca cttcaacgaa cggttttgca tctgggcttg ggctacggta
     6361 gaaagtcccc accattggtg aaagcactgc gtggccactc gtttctgcgg cttttggtgc
     6421 ttcagccgga gctgcaacag gtactgtcga agctacttga ggtgccgctg gtgcaacaac
     6481 aggagctgct gcatattgaa ctgcagctgg tgcaacagct ggagcagcac ggctaatacg
     6541 caccgtacct tcttcttcag acacttctaa ctcggtaatg ccagattctt caactaattc
     6601 aataagtttt ttgattttgc gaatatccat aataagattc cgtatgtgat taaataaaat
     6661 aaaaaagaga actccacatt tggggtataa attcaaatgt agagttaaaa aatgctgggc
     6721 gtagaatacc tgaatttcgc taattttgcg aataaaatct gataattttg gcgaaaatgg
     6781 acgattttag cattcatcgt accagttagg atttttggaa aatggtgact cacaagcgat
     6841 tgaaaattgc caattttttg taaaaaacaa ccgcttgtca gattatttca ataaatcttc
     6901 atttttcttg taattccact tgctaccggg cttgtcgata tactgaaaat attcgttggt
     6961 aaaagtacct tgagcacaag ttactttgtc tcggcggaaa tttacttctt tatcaccaat
     7021 ggcattattt agggtgtcag gaccggtcat aacaaacacg ccaccttcaa tccgacgatt
     7081 ctcaatattg ctgataatca gctcaagggt gtcttttaga atccaatttc cagcttccga
     7141 agccaagaaa aagttggtgt atttatcacg gcgtttaatt aacacttcgc tatcattttc
     7201 gccaataatt ttagagagtg ggtaaaccaa atgcccgtca atatcaatat aaatgccacc
     7261 ttctcggagc aaagtaaaaa ttcgccaaaa atcagcttgt gccgcaccat cggttaattt
     7321 gctataggcg ttaaaggtgc gttcatcggc gttggccttg atgtatgcct ctcgttcttc
     7381 tgtgctaacg tagcgataat cgtagctcaa tgacattagg cggttaaaca gataattcgc
     7441 atacattggc aaggtgactt tgttagaata attcgtttgc caaatggttt tggtaatttt
     7501 gctcggaatg cttgattgga tttttgccgg gctatattcc ggaatggtga aacgtttttt
     7561 cggaaaaagt gcatggaacg gataactcaa tattttgacg atattaccga ataaacggaa
     7621 gactccgtta cagatcacaa taaattgttc atttttaaca gccattttat taccttatgc
     7681 ttttaagtgt gaaattgcaa aatccaacgc atattcatag ccttttgccc ctaaaccgca
     7741 aataacgcct tttgcaatat cacttagata ggagtgatgg cgaaaaggtt cacggctatg
     7801 aacgttagac aaatgcacct caacaaacgg aatcgacaca ctcaataacg catcacgaat
     7861 tgccacgctg gtgtgagtaa atgccccggg gttaataata ataaaatcgg tatgctgaaa
     7921 ggcttggtga atacggttga taatcggctc ttcaccgttg gcttgaaagc tctccagctc
     7981 aacatcttgg gccgcagcca atttttttaa gtggttttca atatccgcca aagtttgcga
     8041 accgtaaatt tgtggctcac gtttgcccaa catatttaaa ttcgggccgt taaggagaag
     8101 gatttttttc atatttactt attcaacttg taattcataa ttaagccatt cacggttttc
     8161 tttcgccccc aagcgttgat aaaaacgaat accaccgtca ttccaaggat tttattgtct
     8221 tacaatgatg tgccttcgcc tcatttttca aggcttgcat taacgcctct cccactttct
     8281 gattccgagc agtttcatca acataaagct ctttcatata aatagcgggg cggttttggg
     8341 cagtgtaagg aagaaaataa taaaccaata tgccgataat gttagacgca ttttcagcca
     8401 ctaaacagta aaaatcgggt gggcttttgc gaaagccgga ttccattaca acttcaggag
     8461 taatcgcaaa agaatcgata tagtgctcaa acacagccag tttttgcatc agagggaaca
     8521 cttggtgggt atcttgttcg accattttgc ggattgtaaa tgccataaaa ttccttacaa
     8581 gcggtaaaaa aatccctcac ttttgcaaag cgagggaggt tttaattatt cgtcaataga
     8641 acgtaataag tcgtttaagc ccactttgct taaggttttc tcatccactt ttttcacaat
     8701 caccgcacag tagaggttgt gtgaaccatc ttttgatgga agcgagcctg aaaccacaac
     8761 agagcctgcc ggcacacggc cgtaatgtac ttcgccggtt tcacggtcgt aaattttggt
     8821 agattgaccg ataaacacgc ccattgagat cacgcagtta tcttcgacaa tcacgccttc
     8881 tacgatttca gaacgtgcac cgataaagca gttgtcgccg atgatggttg ggttcgcttg
     8941 taatggctct aacacaccac cgatgcccac gccaccagaa aggtgaacat ttttaccgat
     9001 ttgcgcacaa gaacccactg ttacccaagt gtccaccatt gtgccttcgc caacgtatgc
     9061 accgatgttc acgaaagatg gcattaacac cacgtttttc tcgatatgtg aacctttacg
     9121 caccaccgca cccggcacgg cacgaatgcc atctgctttg aattgctctt cggtgtagtt
     9181 gccgtatttg gttggcactt tgtcgtagaa tttggtttct gcaccgtcga tgatttcatt
     9241 gtcggcaata cggaatgaaa gtaataccgc ttttttcacc cattggttta ctacccactc
     9301 gccatctttt ttctcggcaa cacgcagtga accgttgtct aatcctgcga tcacctcttc
     9361 tactgctgct ttggtttcag catcaacggt tttcggagta atttccgcac ggcgttcaaa
     9421 tgccgcttca ataatagctt gtaaagacat tgttgtgttc cttcaatata taaattaaaa
     9481 cagaaaattc ggctcacttt ttaacataga aaggcgttta tttccacaag aaaaaacata
     9541 tcgcagaaat ttagtttata attccaagcc acttatttga tacaagcggt gatttttgtg
     9601 gaaaattttg caaatctgct taccggtaaa acgcgtgagc atttagtgcc gttgccgaac
     9661 gctctttcgg ataaacatta ccttcagcct caggtggttg aggctttttt gacgttgcag
     9721 catgctgcaa aacaagccgg ctttaattta cagcctgcca gtacttttcg tgattttgaa
     9781 cgccaaaaag ggatttggaa tgcgaagttt aggggtgagc gtaaggttca cgatgataac
     9841 ggggttgcga ttgatatggc ggcgttatcg gatcttgaaa agtgcaaagc aatgttgaga
     9901 tggtctgcgg tgccgggggc aagtcggcat cattggggaa cggaaattga tatttttgac
     9961 cctgatttat tgccggaaaa tacgccttta ttgcttgagc cgtgggaata tttggaaggt
    10021 ggctattttg ccgatttaac caagtggtta cacgttaatg cggaaacatt tggcttttat
    10081 ttcccgtttg acggtaaaca ttctcgagtg ggctttgagc cgtggcacat tagctatcgc
    10141 ccgatttcaa atgaatatga gcaacgcttt aacgatgaaa tcttaaaagc tgcgtggcaa
    10201 gatgaagaag tggcgggtaa ggaatgctta cttgaacatt ttgaagaatt actaaaatga
    10261 tccaattaat taacgaaacc caacaacacg acaaattccg ccaaatttgc gacaaatggc
    10321 agctggttga agataaatcc agcccgttag ccttagtgct aacggaggaa cgattggaac
    10381 tacgcaaatt agacgaaccc aagctcgggg cgattgcagt gaattttgtg gacggtacga
    10441 tggcacatcg gcgtaaattc ggtggtgggc gaggtgaagc cgttgcgaaa gcggtcggca
    10501 ttaaagggga ttatttgcca actgtgattg atgcgacagc cggtttaggg cgagatgctt
    10561 ttgtattagc agcggttggt tgtaaagtgc tgttagtgga acgcaatcca attgtcgctg
    10621 ccttgttgga agatggttta aaccgagctt atcaagatgt ggaaatcggc agttttatgc
    10681 aagaaagaat gatcttagcc gatgttcgag acatttctca gctagatcca caatcccaaa
    10741 tggctgaggt agtgtattta gatccgatgt atccgcacaa acaaaaaagt gcgttggtga
    10801 aaaaagaaat gcgggtgttt caacatttag ttggggcaga tttagattcg gacagctttt
    10861 tcttaccggc aaaaggattg gcaaaaaaac gtgtggtagt gaagcgtccg gactatgccc
    10921 cgtttttagc ggagcaaaag cctgattttt cacacaccac caaaaatcac cgttttgatg
    10981 tttatctttc tcacctgaaa aatgattaaa ggtgggtcat gcgataggca agcggttatt
    11041 tttacagtaa aatttacaaa ttatgttatc gtttatttat atttaaaaag gcggttatcg
    11101 tgccacaacg tgattatgtc gctcgttcca acgagaaaaa aagtaaaaaa aatcaaaaag
    11161 aaagtaatgt taaaaagatt ttaatgcgga ttatcggggt aattttattc ttcggtattg
    11221 gtttatggtt tttgaaatcc agtgcacctg taccaacagt tccgtcagca ccgcagcctg
    11281 ctaattcaca gactaaaagc gaattaccaa gtcgaccgga agagacgtgg agctatattc
    11341 gtgatttaga aacccgtacg gtaattaccg ataatagcca aacttcacaa gaacgtttgg
    11401 cacaattaac ggcggaacaa aagcagcaaa ttgaagaaag acgcaaacaa gaagaagcac
    11461 gcttagccgc tgagcaagct caacaaaagc ctgcgaccga tgtatctgct acagaattgg
    11521 ctccgaaaac agaggaagtt aagtcgttaa cggaagaaga attagcagca aaaaaagcac
    11581 aggagcaagc tttagcacaa aagcaagcgg aagagaaacg cagacaagca gagcttaaaa
    11641 aacagcagga agcgaaaaaa gagcagccgg ctaaaattgt agcaagtgct gcaacactgg
    11701 cagataaagc tgcaaatcag gcgggtaaat ttgggttaca gtgcggtgca tttaaaaata
    11761 aagcccaagc cgaaaatatg caagcaagat tggcaatggc aggttttaac gcccgtatcg
    11821 caagcagtgc cgattggaac cgtgtttttg tcggcccgat aggtgacaga gcggcaactt
    11881 ccagagctca atccaatgca cgcagtgtag cggaatgtgt gattgtggcg atgtaaaatt
    11941 gattgaaata ttgaagagag ctataggctc tcttttttta tatcccaatc gcatatttca
    12001 aggcttgctt tttgatgggt gacactttat cagccaatac taaaccgaca ttgcgggcaa
    12061 ttttgagcgg taataaatcg gtcttaaacg ctttataaaa cacgtccatg cctgtttgca
    12121 tcagtaaatt atccggttta cgtttttgct catagcgttg aagcacctgc tcacttgcaa
    12181 aatttttgcc ttttttgacc gcttgttcga tcacctcaag caaggtttta acatctttaa
    12241 agcctaaatt tacaccttgt ccggcaagcg gattaatggt gtgagccgca tcgccgacta
    12301 ataccacccc gttttgcaca tagtgctgag cgtgctggcg ggttagcggg aaagcccctg
    12361 aagcctgcac ctgaatgttg cctaattctg cgttgagccg gcttgggaaa tgttgctcaa
    12421 tcgcaactcc cagcttttct gctggaagca ttttcagttc ggcaattttt tgtggtgaat
    12481 cgtaccaaac aacgcagccg tgattgccgg aaagcggtaa aaaggctcga gggccggttg
    12541 gatagaattg ttgccaagtg ccgcttggag caggtttttc ggtggtaacg gtggcgagta
    12601 aacagtgttg gcgatattgc caagcggtta aaccaatgcc cgcccattgg cgaacctgtg
    12661 aatttgcacc gtctgcggca atcagcagtg gggcagcaaa ggtgttgcca tcaaccgtca
    12721 cttcccaata ctcgcccgct ttatttgcaa aaatttgctc aaatccgacc gcttgtaggc
    12781 agtttggata ttgctttaat tgctcccata acgcaatttg aataacgttg ttttcgacca
    12841 tatcgccgag cttttccaaa cccaaatcag ctgccgaaaa aagagtgttg aatccatcaa
    12901 tttcccaggt ttcgagctgg gtataagggg tgcttctcat tgcaacaatt tgctcccaca
    12961 cgcccaattt ttctaacagt tgaatcgagc ccatactaat ggcggaaatt cgcaaatcgt
    13021 agtctgaatc aggattaaag gtcggcagag ggtttttctc caacaaggct aaacgcagcc
    13081 cctgttttgc taagcctaga gctgttgctg ccccaatcat accgccaccg attacaatca
    13141 tatctaattc agttttcatt ttttcttcct ttttagttag gcaaaatact gctaaaacgc
    13201 cttgaattgt agtgcatttc agattaggat caaaccgatt tatctattta tctaaggagc
    13261 gttttatgtc ttgcaaaact catgcaaacc acgagcacga acacggcgaa ggctgtggtc
    13321 atactgctat taaacataac gaccatatcg actatttaca cgatgggcat ttacaccaca
    13381 acaaacacgg cgatcactat gacgaacacg ttttagaagt gaatgaaacc aacccggcag
    13441 actgtaatgc cgcccattgc tgcggcgacc acgttcatgg tgaggtttgc ggacacgaag
    13501 ctgtgccgca cggcgatcat atcgactaca tcgtgaatgg gcgtttgcac caccaacacg
    13561 gcgaccactg cgatgaccac gggccggttg aaattgtgaa ataagatttt tgatgggagg
    13621 ttttagcctc ccattattat ttttgcgatc tcgctcgaaa aattgaacat tcctgctacc
    13681 cctcttccga ttttcctttt atgctctcgg tattgatttc taataccgaa atgtgatgac
    13741 cttagcgatt gtttacagcc gagcctccat tggcgttgaa gccccgttgg tgacgattga
    13801 agtgcatatt agcaacggac aaccaaaatt aaccattgtc ggcttgcccg aagccacagt
    13861 gaaagaggct ggtgatcgtg tgcggagtgc gttgctgaac gctaatttta tctatccgcc
    13921 acaacggatc acgattaatc ttgctccggc agatttaccc aaagaaggcg gacgttttga
    13981 cttgccgatt gcgattggca ttctggcagc ctcagggcaa attgattctg atttattaac
    14041 gcagttcgag tttttgggcg agttggcact gacagggcat ttgcgaggag tgcacggcac
    14101 gattccggca gtgatttctg ccgataaagc caaacggcag atgatcttag ccaagcaaaa
    14161 tgcgaatgag gcatcgcttg tttccaatgc caccacctac tttgccggct cgttgttgga
    14221 ggtggtaaat atgctcaaca aacgggataa actgccgatt tgtcagcaca tttcgcaaca
    14281 ttctgccgaa attcgaccgc ttgtcagccg agatcttact gatattatcg ggcaacaaca
    14341 cgccaagcga gccttaatga ttgccgctgc cgggcaacat aatttgctgt ttctcggacc
    14401 accgggaacg ggtaaaacaa tgttggcaag ccggctggcg gatttactgc cggaaatgac
    14461 cgatgaggag gcgattgaaa ccgcctcggt gaccagcctt gtgcaaaatg agctgaattt
    14521 tcagaactgg aaacaacgcc cgtttcgttc gccccatcac agtgcatcaa tggtggcatt
    14581 agtgggaggt ggctgtgaaa actagaaata aatgtcaaaa acaagaaata aatgtcacaa
    14641 atctttctct aatttcgcta taattcgatc atcaaaactt taaggggaaa tccgttatgt
    14701 tctacctcaa taacgacaat ttcaaacgag aaccttatcg tggatattgt ccgagtcacc
    14761 ttgaaaagct ttttactaaa ccacaccttg atattagagg taacggttat tattaccctg
    14821 acaatgaata tgttgtagct tggtatgaat cggacttcat tttcaaatca agaaaagctc
    14881 aagcacatgc aaaagtaaag cttgttaatt taacttgccc caaatacact ggcaaaaaca
    14941 gtgctgaata tttaagcgtt ttgtatatct ccgttcccct taattatcta cattatttca
    15001 ctatcggcag tatctggaaa ggcggtgtcg ccaaagagca gtttgctctc gaagaatttt
    15061 acattacggt tgaagctgaa aatcaagatg gtaaacgaga aaacctctca atcgtttcat
    15121 tcggtgagag tgaggaaaac ggtttagaga aaccatttga ttacacaatc tacactatcc
    15181 ccacagaata tcacaactac ggtgatgacc taaaccgact actttccatc tcttaccaaa
    15241 atcaaaaatt cattatccat ccgctacata tttttatgat gcactacggg tactctacgg
    15301 acatcaagcg tatcttggca acatacccac ttgacgaggt aagagaacgc ctatttatag
    15361 ataaggtcgt tgagaatttt gatgtagagc gttatgtcgt tttaccgaaa tactttgtaa
    15421 aaaaagatgc gattttcctc tatcacttca aatatgacga agagactacg ggaacaaggg
    15481 taaaaaagct tgtaagccag tttaggttaa atattcacaa tcaaacacat ccaatagaaa
    15541 tagatttttg gcacacgcaa aaggttgaat tgaaaatccg tggtgttcga cttggaaatg
    15601 ctgtgttatg cgcagaaatt atcggtctta atcagcccga aggcgaagat attacgctag
    15661 tgctatctca atcgaaaaga gaaaaatcgg gtaataaaac cgaggaacaa aataacgagg
    15721 taaataacgt tcctattacc agagtttata cacgagaacc tgaattggat gagttaccac
    15781 tcaccgataa ctccccagat aatcgaacag tcgaatacaa caagcgacaa tttgaactct
    15841 tgggccgtca acgccaaatc agagcgctca agaaagcacg cgaagagagt aatcagaaaa
    15901 tgaaagcctt gactccagat gagatagact cactgggtgt aggtgaatca gatggccgaa
    15961 acggtaaggt tggactcgct ttctgtttcc ttgatgatac acctgttggc aaaagctcca
    16021 aactatacaa attatggcag cacgcgaagg ctgttgcgga atggaatcac ggtgaggcgc
    16081 actggtacac gcctaatctc ggttttagaa atgacgatga actccttccc gtttccttag
    16141 aaaccaacaa atgtttcgac taccctgaaa tcgcgataat tatccgcctc caaatatacg
    16201 gagaaacatt tttcctgatt gatttctcaa tgaagcaaag agatataagc attcgtggct
    16261 tagggtataa acccgatcca aacgaagatt ttctctatga aagtgatatg agcaaaagcg
    16321 aattatccga attattgtcc gcagttcatc atcatgaaca tctgccaaaa gagtacatag
    16381 agaaaaagaa caaagaaaaa accaaactca ctacatttaa ccatagcgaa gccgaaagta
    16441 gtaactgggc ttataacgcg gtgcaaaaat taacttcaag ggcaattact aagccgacat
    16501 atttttaatt atttttatga atgcagaaca acgtgtaatt gttactccga cattttgggg
    16561 taaatttttt ggaaaaatta aaagtgttga acttcaacaa aacaaagtta tcgtaactga
    16621 caaaaagaat aatataactg aacatgattt aggaaaaact tttgactttc cagctatcca
    16681 gaagtctttc tttggtacta aattattttt taaagacgat agcacagaag ttgttttatc
    16741 aaaacttgcg aaaaaacaaa ctgatagtct attacttgaa atcgaaaaag tagttgcatc
    16801 taatattaaa gtgaaagtaa aagagggttt tcaacacttt gcaaatttag ccgaaaatca
    16861 atatctcaga gattccgaca ttcctacatt aaatgatcgt gtaagattgt cagtgctttc
    16921 ctatggggat aacaaagaac attttcaaaa atactttgat gaaagtttgg ttaagaaaat
    16981 tcaatatatc tcttcacttt ttcctgtaaa tgccataaat gctcaaacaa ttcgaagaga
    17041 atacgagcaa catgttttaa atcatcgcaa gcatttcttt gatgtcattg aatctaatcc
    17101 gcttactgag caacaaagat tgtcagtcat tcggaacaac gacatcaata tggttcttgc
    17161 tgccgcgggg acaggaaaaa cgtctgtaat ggtcgcaaaa gcattggact taattgattc
    17221 tggtgcttgt aaaagcgaag atattttgat tcttgcatac aaccgcgatg cagcaaccga
    17281 actatccgaa cgcttacaag aacgtgcacg agaagccaat ttagatctgg aaaatagccc
    17341 tagcatctcg actttccatg cgcttggccg aagaattatt aaagaatgtg gaaaatcagt
    17401 ccacttgtca aaatttgccg aagatccgat gaaattagat agatggttca acaaatggct
    17461 agaggactac atcaaagaag atcctgataa tcttgcaaaa ttcattcaac ttgcatacca
    17521 acctatcgat ccattccaat tcaaaacgaa agacgaatac gatagatatg taaatgacaa
    17581 tgactatcga accttacaag gcgaacgcgt tcgtggttat caagagttat tgatcgcgaa
    17641 ctggcttttt agccacagcg tagaatatga gtatgaacca aggtatgtat caaaacgccg
    17701 tatcgagatt ggttttgact acaaacccga tttctctttg ggtgatggtg tttaccttga
    17761 acatttcggc attgaccgac aaggtagaac tcgcgcagat attaacgcgc aggaatataa
    17821 tgccaatatc caaagaaaac gtgagttaca cgccgaacat aatacaacat tactggagac
    17881 ataccactac aactgggtgg aaaatacgtt atataaacga ctagagcaat tgatgaatga
    17941 acagttcatt cctctgaagc caaaatcaca acaagagatt ttagatgcac tcaatgaatc
    18001 aggaatcttc aaagaaaaca aaaatcgcta tttgaagtgc cttcaagcta ttcgtacaga
    18061 gcgattagac tatcaacaaa tcttaaaacg tctaaccgat gcgaaaattg tctatgctaa
    18121 agaatatgcg acattgctca tgcgtattca tgatgcctac gttaaagaac tacgtagcgc
    18181 aaatgaaatc gactttgacg atatgatttt gttggctact caactggtga aaacaggtga
    18241 gtttaaacct aaatggaaac atattttagt cgatgaattt caagatattt ctatggctcg
    18301 attggagtta ttgaaagaaa tttatactaa agggcctcga ccgatttgga ctgttgttgg
    18361 agatgactgg caatcaatct atcgtttctc tggcggtaaa ctagaagtta ctacgcgatt
    18421 taatgatatg gtcggctcac acacgctaag taaactagaa aaaacctacc gctataacaa
    18481 tagtattgct gacacagcag gtcaattcat tatgcaaaat cctgagcagt atcagaaaaa
    18541 tgttgtaacg catacaaagg tcgctgattc ttgtgttcat ttgtatgact ctcatgtcgt
    18601 aaaggatgaa aaatcagaag ccaatatcag tttaaaagcc tcagcaatat taaaactgat
    18661 acgtcaaaaa gcacctgaag ccactgtcgc aattttggct agataccgtt accttttaga
    18721 agatgcaaaa agtgtaatca aagataagaa tgttaaattt tggacgttcc acggctcaaa
    18781 agggttggaa gcagattact gcattttact tggtttcttc caaggaaaag tcggatttcc
    18841 caaccaaaat aaagaagaag ccgttgtaga agcattgctt cctatgctag acagttttcc
    18901 acactcagaa gaacgtaggt tattttatgt ggcattaacg cgtgcgaaaa aagaaagtta
    18961 tttgatcgca gatgcgaacg cgccatcaga atttatcaat gaattattat caccgaaata
    19021 caagatagat attgtgtcaa aacgattcca agaacgttat cggacaatct ttaaatgtaa
    19081 tgaatgtagc actggctatt tcgtgatgaa acaaggtaaa tttggtcagt tctattcttg
    19141 tacatcaggg gaagtatgcc gctcttcgcc acggatttgt gaaaaatgtg gccgtcctat
    19201 gaagctgcga gaaggtaaat ttggtcagtt ttggggatgc tcaggatatg gaatcaaagg
    19261 cgatcaa
//
LOCUS       DANLXQ010000032        18973 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.032, whole genome shotgun sequence.
ACCESSION   DANLXQ010000032 DANLXQ010000000
VERSION     DANLXQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18973
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.032"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            31..714
                     /locus_tag="PXD94_000867"
     CDS             31..714
                     /locus_tag="PXD94_000867"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL6285753.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGISDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            complement(851..1408)
                     /gene="frr"
                     /locus_tag="PXD94_000868"
     CDS             complement(851..1408)
                     /gene="frr"
                     /locus_tag="PXD94_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL6285754.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
     gene            complement(1460..2176)
                     /gene="pyrH"
                     /locus_tag="PXD94_000869"
     CDS             complement(1460..2176)
                     /gene="pyrH"
                     /locus_tag="PXD94_000869"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL6285755.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIEADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVFGTEEGTTISE"
     gene            2268..3830
                     /locus_tag="PXD94_000870"
     CDS             2268..3830
                     /locus_tag="PXD94_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL6285756.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNGIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            complement(3894..4745)
                     /gene="tsf"
                     /locus_tag="PXD94_000871"
     CDS             complement(3894..4745)
                     /gene="tsf"
                     /locus_tag="PXD94_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL6285757.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGGNA"
     gene            complement(4841..5560)
                     /gene="rpsB"
                     /locus_tag="PXD94_000872"
     CDS             complement(4841..5560)
                     /gene="rpsB"
                     /locus_tag="PXD94_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL6285758.1"
                     /translation="MAQVSMRDMLNAGVHYGHQTRYWNPKMKPFIFGARNGVHVINLE
                     KTLPLFNEALAELTRIASNNGKVLFVGTKRAASDAVKAAAVESQQFYVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDA
                     IFVIGADHEHIAIKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAV
                     AAIKEGRGVETVEVAEEASAE"
     gene            5905..6933
                     /locus_tag="PXD94_000873"
     CDS             5905..6933
                     /locus_tag="PXD94_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6285759.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLARKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEHGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSAIVKGWGTFKSDTIKLEDIAKNYEAALKLVDEVK
                     FDDFSEKK"
     gene            7056..8702
                     /locus_tag="PXD94_000874"
     CDS             7056..8702
                     /locus_tag="PXD94_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6285760.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            8712..9722
                     /locus_tag="PXD94_000875"
     CDS             8712..9722
                     /locus_tag="PXD94_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6285761.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVGKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            9732..10358
                     /locus_tag="PXD94_000876"
     CDS             9732..10358
                     /locus_tag="PXD94_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL6285762.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAESKDLGEAMVERLFKAYFTDNTILAKRTELISLALDIGLERDEIAQLLTGDDFG
                     HEVREDERVAHKYGIHSVPFFVINEKLGVSGAQPPEILLDAIKQALQK"
     gene            complement(10420..11910)
                     /locus_tag="PXD94_000877"
     CDS             complement(10420..11910)
                     /locus_tag="PXD94_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDL6285763.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGSVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRITISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSKGVGVYFVTQNPLDIPDSVLGQLGNRIQHALRAFTPRDQKAV
                     KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIAYIYPPKSQLAPLAT
                     GERDSLVKQDDLYHHYSQYVDNESAFELLNAKAEAIKAEQAAAEEANNDFFGGILSSI
                     FGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAITKK"
     gene            complement(11969..13204)
                     /gene="rhlB"
                     /locus_tag="PXD94_000878"
     CDS             complement(11969..13204)
                     /gene="rhlB"
                     /locus_tag="PXD94_000878"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL6285764.1"
                     /translation="MSNHLTEHRFIDFPIHANVLNALDSKGFEFCTPIQAKTFPITLK
                     GKDIAGQAQTGTGKTLAFLIATFNHLLNNEIETSKNQPRALILAPTRELAVQIGADAE
                     LFLKHSDLKLALAYGGDGYDKQVQEIEKGVDILVGTTGRVIDYVKQGIINLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPKPSERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQKTGHRIKEELFYPSNEDKMALLLTLLEEEWPERCMIFANTKHKCEEIWQYLSAD
                     GHRVGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            complement(13292..14014)
                     /gene="recO"
                     /locus_tag="PXD94_000879"
     CDS             complement(13292..14014)
                     /gene="recO"
                     /locus_tag="PXD94_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL6285765.1"
                     /translation="MTIDQWQRAFVLHRREYSENSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLLRWSGKGDLKTLTKAEPAALTLPMETLSLYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCITELATQKQIEPILRTFEFHTLKALGYGVDFTHCAATGLPVD
                     PKMTYQFQENQGFIASLLQNNQSFLGSDLLAFAQLDFSEKSTQQAAKRFTRMALKPYL
                     GSQPLKSRELFQSILPNKLKSG"
     gene            complement(14244..15161)
                     /gene="era"
                     /locus_tag="PXD94_000880"
     CDS             complement(14244..15161)
                     /gene="era"
                     /locus_tag="PXD94_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL6285766.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILGQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
     gene            complement(15264..15938)
                     /gene="rnc"
                     /locus_tag="PXD94_000881"
     CDS             complement(15264..15938)
                     /gene="rnc"
                     /locus_tag="PXD94_000881"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL6285767.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKANEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            complement(15984..16943)
                     /gene="lepB"
                     /locus_tag="PXD94_000882"
     CDS             complement(15984..16943)
                     /gene="lepB"
                     /locus_tag="PXD94_000882"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL6285768.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKDPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            complement(17030..18823)
                     /gene="lepA"
                     /locus_tag="PXD94_000883"
     CDS             complement(17030..18823)
                     /gene="lepA"
                     /locus_tag="PXD94_000883"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL6285769.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEIDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKMPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTTELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     INNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
BASE COUNT         5527 a         4053 c         3500 g         5893 t
ORIGIN      
        1 attaatttta ctcaaagcta aggagtcatt atgcaattag tatttatccg tcacggtttc
       61 agtgaatgga atgctaaaaa cttatttacc ggttggcgtg atgttaattt aacagaacgt
      121 gggattgaag aagcaaaatc tgccggtcaa aaattaaaag cagccggttt tgaatttgat
      181 attgcgttta cttcggtttt aacccgtgcg atcaaaactt gtaacatcgt attagaagaa
      241 tcagaccaat tatggatccc tcaagtgaaa aactggcgtt taaacgagcg tcactacggt
      301 gcattacaag gtttagacaa aaaagaaact gctgaaaaat atggtgatga acaggttcac
      361 atctggcgtc gttcttatga catctctcca ccggatttag acccacagga tccaaactca
      421 gcacataacg accgccgtta tgctaactta ccaaaagatt taattccaaa tgcagaaaac
      481 ctcaaaatca cccttgagcg tgtgttacca ttctgggaag atcatattgc acctgcatta
      541 atttcaggta aacgcgtatt agttactgct cacggtaact cattacgtgc attagcaaaa
      601 cacatcgaag gcatttctga tgcagacatt atggacttag aaatcccaac cggtcagcca
      661 ttagtgtatg aactagatga caacttaaaa gtaatcagca aacgttactt ataattggta
      721 aatttccgat aaaaaataac cgcttgtagt gcaacacatt acaagcggtt attttttgtt
      781 aattttttgc aaatggcaaa gaacaaaaat aaaggcgaat catacattcg ccttattgtt
      841 ttaaagtaga ttagaaatct aacaactctt tctcttttgc tgctaatact tcatcaactt
      901 ttttaatgaa ggtatcggtg attttttgaa tatcttcttc agctttgtgt tgttcgtttt
      961 cgctgatttc tttatctttt aataacgctt tgatttggtc attagcatca cgacgaacat
     1021 tacgaattgc cactttgcct tgctcacctt ctgctttaac gattttgatt aaatcacgac
     1081 gacgttcttc cgttaatggt ggaagcggaa cacgaatggt tgtgccggca gaagatgggt
     1141 ttaaacctaa atctgaggtt aaaattgctt tttctaccgc agagattaaa gatttatcaa
     1201 atacgtttac cgctaaagta cgggcatctt cagctaccac gttcgctact tgacgtaatg
     1261 gagtcgcaga gccgtaatat tccacttgaa taccgtctaa taaagaaggt tgggcacgac
     1321 ctgtacgaat tttcgcaata tgccctttta aagcctcaag gcttttttcc atacgatctt
     1381 gggtatcttt cttgatttcg ttaatcattg attttttatc cttttattta gtaactgaga
     1441 gttaaacaga gaaaattaat tattcagaaa tggttgtacc ttcttcagta ccgaatacca
     1501 cattgcgtaa cgcccccggt ttgcccatat taaagacacg aattggcatt ccgtggtcac
     1561 gagcaagcgt gaatgctgct aagtccatta cttgtaattc ttgctcaata acttgcgcat
     1621 acgttaattt attatatagt tttgcatctg cgtattttgc tgggtcttta tcataaacgc
     1681 catcaacttt agttgctttt aataccacat cagcttcaat ttcaatacca cgcaagcaag
     1741 cagctgaatc ggtggtaaag aacgggctac ctgtaccggc agagaaaatc accacacgtt
     1801 tttcacgcaa cattttaatt gcttcagacc agttgtaagt gtcgcaaatc ccatttaatt
     1861 ggaacgcaga cattaattta gcatttacct ctgcacggtg taaggcatca cgcatagcca
     1921 aaccgttcat tacggtggca agcataccca tatggtcgcc cactacacgg ttcattcctg
     1981 ctttggctaa ttttgcacca cggaataagt taccgccgcc aagtaccaca ccaacctcaa
     2041 cgccttcagc aagtaattct ttaatctcta acgccatacg atccaaaata gacgggtcga
     2101 taccaaaacc ttcactgcct tgtagggctt caccacttaa tttgagaaga attcgcttat
     2161 aaataggttt gctcatttta attgcctctt aactgtatgg actaaacaca aaattacgtt
     2221 attatagaac aatccaatat aacgataaac taaaaatatt cactacaatg aaatccaaac
     2281 aacttttcgg cattcttgtc gctatttcgc tggcaactgc tgctagctgt ttaatgctaa
     2341 tcggatcagg cttttttcca aatccgagta tctttgaagt cattttgggc gtattcctca
     2401 tcatcgctct tgccagctct aaatggactt attatttact cttactgcca atcagcatcg
     2461 cttatgcaat ttataccccg gttgggataa aattcggtaa accgacttac gagtatatcg
     2521 cctctatttt tgctaccgat atgatggaaa gcaaagagtt tattgctcaa ctgccgattt
     2581 taaatattgc ggcgggcatt gcgattgtac tagcggtcat tttttatcga aaaattacca
     2641 atcgctatca aatccgtttt ttacgcaata aaacattcgt gatttcggct tttgtattaa
     2701 tgctgttttc ccttgcaccg tttaaatttt ttcaggagtt ctacaattcg agcctaaagg
     2761 taaagaaaga attggaagtg ctgaacaatt taacgctaga ttcacaatgg ggacaatcaa
     2821 ccctcagctc aacttcaaac tacgatgatt atgtactgat tatgggcgaa agtgcgagaa
     2881 aggattatca ccacgcctat ggctatccgg ctgccaatac gccttttatg agttcggcaa
     2941 aaggaacctt gattgacggt ttaacctccg gtggcacaaa cactatcgcc tcattacgct
     3001 taatgctcac taagcccgat acggaaaaat gggaagcaaa ttatgggtta accttaattg
     3061 atttaatcaa atctaccggt atcaagacct attggatctc aaatcaaggc tatttgggca
     3121 attacgatac gccgatttcc tctatcggta ataaaagtga tgtcaaaatt ttcactaaag
     3181 ccggcgattc attaaatacc aatgtgagcg attttgaatt gttaccgcat tttgctaaag
     3241 tgattgaaga acctgctcaa gggaagcgtt tcatctttct gcatttatat ggctcgcatc
     3301 ctattacctg cgatcgtctc accgattacc cgaaaatgtt tgacgatgac aaactgccaa
     3361 aacgctactt taatgtaaat tgctacgtgt catctatcaa gaaaaccgat gaattgctga
     3421 aacgggtata tgatttgcta gtgaaaaacc aagaaaatca ccagcgttca ttctcaatgg
     3481 tttatttgtc tgatcacggt ttatcgcacg aggtttcaaa agagaacatt gttatccaca
     3541 atggaaagga tagaagtaaa ttacactacg atattccgct gtttaaaatt tcaagcgatg
     3601 atacccaacg taatgagtat aaagccttta aatccggctt aaacttcact aatggtattg
     3661 cgaattggat tggtatcgaa aatccgttat tagataaaga aattaatctt tttaacgaac
     3721 aaaacgataa aagtgattat gggctaaaga aaatcattga aacgcagtct actgtagaag
     3781 atccagctgt tcctattcct gaaagctact tatataaagc gactaattag caaaagtaag
     3841 taacaaaaaa gcaggcttta agcctgcttt tttagttttt agtatcagaa aaattaagcg
     3901 ttaccgccag tgattttcgc tacttctgct gcgaaatctt cttctacttt ctcaatacct
     3961 tcacctactt ctaaacgaat aaagttagaa actgaagtat ttactgattt taaatattga
     4021 cctacagatt gagatggatc cattacaaac ggctgaccgg ttaatgaaac ttcaccggtg
     4081 aatttagcca tacggccttc aaccattttt tctgcgattt cttttggttt accagaattc
     4141 atcgcaattt caatttgaat ttcacgttct tttgctacta catcagcaga aacatcttct
     4201 gggtttacaa attcaggttt gcttgcagca acgtgcattg ctactttctt aagctcatct
     4261 tcagagcctg cacctgctac taatacacca attttcgcac cgtgtaagta ttgagcaata
     4321 acttcgcctt ctaagtattg aacacggcga atgttcatat tctcaccgat tttcgcaact
     4381 aatgccgcac gtttttcttc aaattgtgct tgtaatgctt cgatagttgt acctttattt
     4441 gcaactgcaa aatcagcaac ttcgttcgct aattctaaga aaccggcatc ttttgcaacg
     4501 aagtcagttt cacagttcat ttcaactaat acgccaaaac ctgaagcgac acgagcaagg
     4561 attacacctt cagctgcaac acgacctgct tttttagccg ctttcgcttg accggattta
     4621 cgcatattat cgattgctaa ttcaatatca ccgttcgctt caactaatgc ttttttacat
     4681 tccatcatac ctgcgccggt acgttcacga agttctttaa ctaatgatgc tgtgatttca
     4741 gccattttaa attcccctta tttgatatgg taaaacaggc tctcacctgc ctagaaattt
     4801 cattgtttag ggcaggttaa aacctgcccc ctcaactgaa ttattccgca gaagcttctt
     4861 ctgctacttc aacagtttca acaccgcgac cttctttgat tgcagcaact gctgaatcta
     4921 agtaaagttg gatagcacgt gttgcatcat cgttaccggg aatgatgtag ttaacgccat
     4981 ctggagttga gttagtatca acaatcgcaa ataccggaat acctaagttg tttgcttctt
     5041 taattgcgat atgttcgtga tcagcaccga taacaaaaat tgcatccggt aaaccgttca
     5101 tatctttgat accgcctaag cttaactcta atttttctaa ctcacgagta cgcattaacg
     5161 cttctttttt agtgattttg tcaaaagtac cgtcttgagt ttgagtttct aaatctttta
     5221 agcgtttgat tgattgacga actgttttcc agttagtcaa cataccacct aaccaacggt
     5281 ggtttacgta gaattgttgg ctctctactg ccgctgcttt aacagcatca cttgctgcac
     5341 gttttgtacc aacaaataat actttaccat tgttactagc aatgcgagtt aattccgcta
     5401 atgcttcgtt gaataaaggt aaagtttttt ctaagttgat tacgtgaaca ccattacgag
     5461 caccaaagat gaatggtttc atttttgggt tccagtaacg tgtttggtga ccatagtgaa
     5521 cgcctgcgtt aagcatatcg cgcatagaaa cttgtgccat aagaattttc cttcaaagtt
     5581 gtttgttttg ccacttatga acggcaaaac ggggtttagc ctccacatat cctagaaaat
     5641 caatctgtta aagacacccc aattcctagc ttgtgatatg tgtgtgtttt agtgttaaaa
     5701 aataaatgtg tacgttttgc ctgaatagac aaaaacgggg cgtattatgc caaaaaacaa
     5761 acaatttgcc aattatttta ttgctctctt cgcattacta aatcaagctc aaaaataaaa
     5821 accgttctca ttattgacta aaaaaatggg gaggtttaga atgtctttca ttctgttatg
     5881 aaaacctgtc tgaaggagac tatcatgaaa aaaacacttt ctgcacttgc tattgctgca
     5941 gccactttta catcaacttc aacgcttgct gcaaatgaag tgaatgttta ttcttaccgc
     6001 cagccttact taattgagcc aatgctaaaa aactttgaaa aagatactgg cattaaagta
     6061 aacatcatct tcgctgataa agggttggtt gatcgtgtaa aacaagaagg tgaattaagt
     6121 cctgctgatg tgcttttaac agtagatatt tcacgcgtaa tggaaattgt taacgctgat
     6181 cttgcacaaa aaattgactc taaagtgtta gaaaaaaata ttcctgcaca attccgtgac
     6241 tctaacgacc aatggtttgg tttaaccaca cgtgctcgtg ttatctacac gtcaaaagat
     6301 cgtgtaggta aattaccagc aggctttgac tacctagatt tagctaaacc agagtataaa
     6361 ggtaaagtct gcgtgcgttc aggcaaaaac tcttacaatg tctcactatt cgcggcgatg
     6421 atcgaacatt acggcattga aaaaaccaaa gcattcttag aaggcttaaa agcgaactta
     6481 gcacgaaaac cacaaggtgg tgaccgtgat caagtaaaag caattaaaga aggcatctgt
     6541 gattactcta tcggtaacag ctactactac ggcaaaatgt tagatgatga gaaacaaaaa
     6601 tcgtgggctg aagcagcgat tattaatttc ccgagtggtg aacacggcac gcacaaaaac
     6661 atcagcggcg tagttattgc aaaacactca ccaaataaag caaacgcggt gaaattaatt
     6721 gaatacctaa gtggcgaaaa agcacaaggt ttatatgctg aacttaacca cgaatatcca
     6781 gtaaaagaag gcatcgagcc atcagcaatc gtaaaaggtt ggggtacgtt caaatcagat
     6841 accatcaaac ttgaagatat cgcaaaaaat tacgaagccg cattaaaatt agttgatgaa
     6901 gtgaaatttg acgatttctc tgagaaaaaa taatcaaccg ataagcggtc atttttccac
     6961 aaaaatttgc aaaattttgt taaaatacaa ccgcttgcaa aaggacgcat tttgcgtcct
     7021 tttatttttt tatgaaataa caggcttaaa gaaaaatgcg atcacaattt tgctggaaaa
     7081 gtattgctgc cctaacaact tttatcgttt tgcttcctct tattgcaatt tgctatcacg
     7141 cattgcaagg ggataatgaa aaccttgctc acctttggca aacaatgttg ccaatctatc
     7201 ttaaaaactc aactttatta gtattaggaa ccgttttcct tgcactgatt tttgctctgc
     7261 ctaccgcttg gattgtggca aattaccgtt tttgggggca aagaacctta caatggttac
     7321 tttgtttgcc tctcgctatg cctgcttatt taattgccta tttatatacc gatttattag
     7381 attactcagg tgcttttcaa ggctggctgc gtagtatttt tggctggact catccgcaag
     7441 attactggtt tcctcaaatt cgtacattgt atggtgcttg ctttgtgctt gcgttagtac
     7501 tatatccata cattttttta ttagtgcgag ttgccttatt agaacaatct gaaaacttaa
     7561 cacacagtgc aaaaatgctc ggggcttcgc cctctcagtt gttccgtcga attacgctgc
     7621 cattaattcg accttctgtt gccgtaggca ttgcattagt ggcaatggaa acgcttggcg
     7681 atttcggtac tgtggcctat tttgccgtgc caacacttac tacggcaatt tatgattcgt
     7741 ggctaggcta tcacgatctt ggtactgcca gccagattgc gatctttatg ttgctaatga
     7801 ttttcctgtt tttaagttta gagcaatata gtcgccgtaa acaaaaaagc tatcaacgtg
     7861 gctatgaaaa gaaatcagcc tttaaatttt taactggttg gaaattagca ttagcccttg
     7921 cttggtgctg gggtttattg gcgttagcct ttttcattcc gtttggcagg ttgctctatt
     7981 gggcatacga ctatttcgag caagcgtgga atttagactt tgtccaattt tcgcttaata
     8041 gtttgaaagt atcgatcatc gcctccgtga taacggtact gattgctcta cttctgcatt
     8101 tttctaaacg tttgaatacc cgcaaaattt tagtcaaaaa tgaccgcttg tcattacaac
     8161 ttgcctcact gggctatgcg gttcctggca cagtattagc gatcggttta ctttcgcctt
     8221 taacatttgc cgatcatcaa ctccatgctt ggctaaaaag ccttgaatta tcacccgttg
     8281 gattgatttt ttcaggctca ctctttgcat tagtattagc ttataccatc cgatttttag
     8341 caatggcaat tggcagcctt gaaacttcgc taaataaaat ctctccatcg ctggatatgg
     8401 cgagccaaac gcttggtaaa aatggctcag caatgttagt aaaaattcat atccctttaa
     8461 tttataaagg tattttaact gcaggattaa tggtctttat tgagagtatg aaagagctaa
     8521 atgcttctct gctacttcgc ccatttaatt ttgatacact cgcaacctat gtgtttacct
     8581 ttacatcaga cgagcagctt gagcgtgctg ctctgcctgc catcgtattg gtgttagtcg
     8641 gtttactgcc tgttatttgg cttaccagat cccttatttc gcaatctgaa aaagaaagat
     8701 aaaggaattt tatgtctgtt ttaactatta atcatctcaa cctcaatttc ggcagtacaa
     8761 aggttttaca aaatctccat cttgctgtga atcaaaatga aattatctgt ttgctaggtg
     8821 caagtggctg tgggaaaact acactgctca aagccattgc aggtttattg ccaattgagc
     8881 agggagatat tcatttagcg ggtgaatgcc taaaaagtaa agccgtggaa gagcgacaaa
     8941 tcgggctgat tttccaagat tacgctcttt ttccacactt gacggtagcc gataatattc
     9001 aatttggctt atccaaattg ccaaaagtac aacaaaaagc aattaccgaa aaaatgcttt
     9061 cagttgtgca tttacaaggc tttgaaagcc gttttccaca cgaactttcg ggcgggcaac
     9121 aacagcgtgt cgctattgcc cgagcattag cctgccagcc agaattgcta ctgttagatg
     9181 agccgttttc caatattgac agccaaactc gctatgcaat gattcaagag atcaagcaaa
     9241 tcttaaaaag ccaaaatgtg cccgctattt ttgtaaccca tagcaaagaa gaagcctttg
     9301 catttgccga taaaattgcc gtgatggatc aagggaaaat tattcaattt ggtaagccaa
     9361 acgagctata ccattctcca gttaatccat ttgtggcaga ttttttaggt ggcacaaact
     9421 atcttaactg taatgtaacg gaagacaacg tgttatgcag ccctattggc gagttccgac
     9481 tcttccctga aatgaacgtt cagaaagggc gttaccgttg gcttcttcgc ccagaacaga
     9541 ttatcttaaa acccaatgag ctaggtttag gtaaagtggt aggcaaactg tttttaggcc
     9601 tatactaccg ctatcaggtt gaaattaaag gcattgagat agtaacttac caatctcaag
     9661 agctgaaaat tggcgaacaa gtggaagtgg gcttccaagt aagagagttt atcctatttt
     9721 aaggagaata tatgaaaatt gaagtttggt cagattatgc ctgcccgttt tgttacatcg
     9781 gcaaacgcca cttagagcaa gccctagccc aatttgaagg cgaggttgaa gtggttttcc
     9841 gagcctttga gcttgatccg cacgctaacg gcgaaccgga gggcgatatt cagcaacgcc
     9901 taatgcggaa atatcaaaaa accgcagaac aagctgatga aatgattcgt tatgtggaac
     9961 aagccggcaa acaagctggg ctagatttgc gttaccgcac cacccaatac actcgcacct
    10021 ttgaagctca tcgcttggcg aaatttgcag aaagtaaaga cttaggcgaa gcaatggttg
    10081 aacgtttatt caaagcctat tttaccgata acactattct agctaaacgc acagagctta
    10141 tcagtttagc cttagacatt ggcttagagc gtgatgaaat cgctcaactg ctcacaggtg
    10201 atgatttcgg gcacgaagtc cgtgaagatg agcgagtcgc tcacaaatat ggcattcaca
    10261 gcgtaccgtt ttttgtgatc aatgaaaaac tcggggtatc cggggctcaa ccgccagaaa
    10321 tattacttga cgctatcaaa caggcattgc aaaaataatc cgaaaaaaga ccgcttgtta
    10381 agctaaacaa gcagtcttat tcttcttata atttacaatt tattttttgg tgatcgcacc
    10441 taaaatacca cgcataattt gtttggtgac ttggttgcgt aaatttctgc caactgaatg
    10501 cgccacttgg ctaaccactt gttcggtaat cgattgatct tttttcttct tcgagccaaa
    10561 aatggaagag agaataccgc caaagaagtc attgtttgct tcttctgccg cagcctgctc
    10621 tgctttaatt gcttctgctt tcgcattaag taattcaaac gcagattcat tatcgacata
    10681 ttggctgtag tgatgataga gatcatcttg cttcactaaa ctatcacgtt cgcctgtggc
    10741 taatggagct aattggcttt ttggtggata gatataggca atttctacag gcgttggcat
    10801 acctttttca tctagcaccg aaaccaacgc ttgccccaca ccaagttgcg tgatggcttc
    10861 taccacattt acctctttat tcgctcggaa agtttccgct gctgatttta ccgctttttg
    10921 atcacgtggc gtgaaagcac gcaaagcgtg ttggatacga ttgccaagct gccctaatac
    10981 tgaatcaggt atgtccaacg gattttgcgt aacaaaataa acacccaccc cttttgagcg
    11041 aattaaacga accacctgtt caattttatc caccaatgcc ttaggggcat cgtcaaacaa
    11101 taaatgagct tcatcaaaga ataaaacaaa cgtcggtttt tctgggtcgc caacttctgg
    11161 caaattctca aagagctctg ccataaacca aagcaggaat gcactgtaca ctcttggtga
    11221 gttaattaat ttttctgcat tcaacatatt gattacacca cgcccattac gggtttggat
    11281 ccaatcgtgc aaatctaatg caggctcacc aaataaatta tctgcccctt cattttcaag
    11341 ccttaataac gcacgctgaa tagctccaac gcttgctgca gaaatatgcc catattccag
    11401 ttggaattct ttcgcattat ctgcgacaaa tttaagcaac gcacgcaaat cttttaaatc
    11461 aattagcaat aaacctctgt catctgccac acgaaaaacg agatttaaca agcctccttg
    11521 ggtatcgttt aaattgagta aacgagaaag taacatcggt cccatttcgg aaatagtgat
    11581 tcgaagtggg atacctgatg taccaaatac gtcccaaaac gaaactggat agccctttaa
    11641 gtaatcttct ccccctaaat taaactgtaa aatgcgttct gcaatttttc ctgaaaaatt
    11701 acccgcttgt accgagcccg aaagatcacc ttttacatct actaaaaata ctggcacgcc
    11761 atcatcactc aaagattctg ccaatttgcg taaagtaacc gttttacctg tacccgtagc
    11821 cccagcaatt aaaccgtgcc gatttgccat tttggcatta agcgaaataa ctttgcctgt
    11881 ttctgttcga gcaatttgat acaaactcat ctttcattct cttaaaggta aattaatttt
    11941 ttgttctcat catataaaaa tttatcactt aattacggct attctttttc gtttttgcca
    12001 agaacgtttt tttacttctt gagcggttca ctttcggtaa agtcagtaag gattccttgt
    12061 cgtattgact cacaggaatc tgatgcccaa tatactcttc aattgcaggc aaattaaccg
    12121 cataacgctc gcaagcaaaa ctaattgaat ggccgctctc ccctgctcgc cccgttctac
    12181 caatacgatg aacgtaatct tctttatcat caggcagatc ataattgaaa acgtgcgtga
    12241 catctggaat atgcaatcca cgagccgcaa catctgttac cactaaaata tccaaattgc
    12301 ctttggtgaa attatccagc aaagaaaggc gttttttctg cggaatatcg ccggttagca
    12361 ttccaacccg atgtccgtct gccgataaat actgccagat ctcttcgcat ttatgcttgg
    12421 tgttggcaaa aatcatacaa cgctccggcc actcttcctc aagcaatgtc agcaacaatg
    12481 ccattttgtc ttcattcgag gggtaaaata gctcttcttt aatacgatgt ccggtttttt
    12541 gtagtggctc aatttcaata tattccgcat tatccatatc ttcataggca agttcccgca
    12601 cttttgccga aagcgttgcc gaaaagagca aagttaaacg ttctgaaggc ttaggtgctt
    12661 ttcgcattaa ataacggata tctttaataa aaccaagatc aaacattcga tctgcttcat
    12721 ctagcacaat cacttgaatt ttgtctaaat tgataatgcc ttgttttaca tagtcaatca
    12781 ctcgacctgt tgtaccgaca agaatatcta cccctttttc aatttcttga acttgtttgt
    12841 cgtaaccatc accaccgtat gccaaagcta gttttaaatc agaatgtttt aaaaacagct
    12901 cggcatctgc cccaatttga accgccaatt cacgggttgg agcaagaatt aatgctctcg
    12961 gctgattttt tgacgtttcg atttcgttat tcaataaatg gttaaaagtt gcaatcaaaa
    13021 aggcaagcgt tttccctgta cctgtttgag cttgccctgc aatatctttg cctttcagcg
    13081 taatcggaaa ggtttttgcc tgaatcggcg tacaaaattc aaagcctttg ctatcaaggg
    13141 catttagtac attagcgtga atcgggaagt caataaagcg gtgttcggta agatgattag
    13201 acatagtatt tccttgataa ttgtgatgtg acaagcggtc ttttttacac atttttttgc
    13261 aaaatagcat attgggcgtt ttttctcaaa tttaaccgct tttgagttta ttcggcaaaa
    13321 tagattggaa aagctctcgg cttttcagcg gttgtgaccc taaatagggc tttagtgcca
    13381 ttcgggtaaa acgtttcgct gcttgttgag tacttttttc tgaaaaatcc aactgagcaa
    13441 acgctaataa atcactgcct aaaaagctct ggttattctg taataatgag gcgataaagc
    13501 cctgattttc ctgaaattga taggtcattt tcggatcaac tggcaaaccg gttgccgcac
    13561 agtgcgtaaa atccacccca taacccaatg cttttaacgt atgaaattca aaggtgcgta
    13621 aaatcggctc aatctgtttt tgtgtggcaa gctcggtaat gcaacgtaaa taatgctgaa
    13681 ataattcagg gtaagcggtc tgattttcca atactcttgc aagtacctcg ttgatataaa
    13741 acccactata aagtgaaagc gtttccatcg gcaacgttaa tgcagccggt tctgctttgg
    13801 ttaaagtttt cagatcccct ttacccgacc aacgcaacaa caacggcgtg aacggctgta
    13861 aaaccgcttt caacggcgag cgagcccgcc ttgcaccttt agcaagtaaa gtaatccgcc
    13921 cgtgatgttc ggtaaaaaac tccactaata aactgttttc gctgtattca cgccgatgaa
    13981 ggacaaaggc tcgttgccat tggtctattg tcataatttc ttgttaatta tgaggttgta
    14041 atttacttct gccaaatctc cccctcccct ctttgctaaa gaggggagat cttgcagagt
    14101 atattttaaa gtcattttca ctatttaacc ttttatatag ataggaagat gaatttaaaa
    14161 tttctggaga actagtcccc ctctttagaa aagaggggtt aggggagatt tgattacgat
    14221 attaatgcta taacgggagc caactactcg tccatatacc ctaaacttct caatgctctt
    14281 tcatcatccg cccaaccagc ttttactttc acccaaagct caaggtgcac ttggttgtca
    14341 aataggcgtt ccatatcttt tcgagcttca atgccgatgg ttttgatttt ttgccccccc
    14401 gcaccaataa ccattttttt ctgaccatct cgttctacta aaatcaagcc attgatttca
    14461 tacaccccac gctcattcat tttaaattgt tcgatttcta ccgttaccga ataaggtaac
    14521 tcttcacctg taaaacgcat tagtttttca cgaataattt ctgatgccat aaagcgttga
    14581 gagcggtctg tcacatactc ttcaggaaag tggtgctcac cttgacgcag tgattgacgg
    14641 acaaatttct gtaaaatatg cacattttta ccccgttgtg ccgaaattgg cagaatttca
    14701 gcaaaatcaa atttttgtga aagctcggta atatgcggca gaagttcttc tttttccttg
    14761 atgttatcaa ttttattgat tgccaataaa acaggggctt ttgccgaacg caatttgttt
    14821 aacaccattt catcatcttc tgtccatttt gtgccttcca caacaaagat gatgagatct
    14881 acatcgccaa tagcagaact tgctgcacgg ttcattaaac gattaatcgc acgtttttct
    14941 tctatgtgta agcctggggt atctacataa atcgcttggt attgatcttc agtgtgaata
    15001 ccaacaatac gatggcgagt cgtttgagct ttgcgtgagg taatggaaat tttttgtcct
    15061 aaaatcttat ttaataatgt tgatttccct acatttgggc gaccaacaat cgcaataaaa
    15121 ccacaatagg tttttgcagg agtttgaatt tgttctgtca ttttgttttc ctttcattct
    15181 gtggaatgaa tttgatggtg caagcgtcca cgcttgtacc atttaattaa atctttagca
    15241 caagcgagga cgcttgcgct atcttacttt ttcgttttaa ccaccgctaa cgctttttct
    15301 gcggcatttt gttcggcttt tcggcggctg gtgccaatgc cgataaacac ttcctcgacg
    15361 ttaatcactt tacaggttac acggaaagtt tggttatgag cttcgccttt aatatctaat
    15421 acatcatatt caggcaatgg caattttcta ccttgtaaaa actcttgcag gcgggttttt
    15481 ggatcttttt gtgaatcgcc tggactgata tctttaagca aatctgaata ccaatcttcc
    15541 accttttcca ttgcttttgc catatcttga tcgagataaa tggccgcaat aatcgcttca
    15601 acacagtccg ataaaatcga ttctcggcga aaaccaccac tttttagctc tcctggacct
    15661 aattttaggt agtcgcctaa atcaaatttg cgagcaataa tcgctagagt ttgctcacgc
    15721 acaagagttg ctctcatacg gctaagctca ccttcattcg cttttggaaa tttgtcgtat
    15781 aatactttac caatagtaaa atttaaaatt gaatcgccta gaaattcaag acgctcattg
    15841 tttttggttg ccgcactgcg gtgtgttaag gcctgaatga ggtaatcaag gttatgaaat
    15901 tgataaccta attttttctg taatcgttct agttgcatag tttatgtgaa taaaggggcg
    15961 tatctcatac gcccctgtta agattaattg attgaggtaa acattctaga aaaacgaagc
    16021 cccgttggga attcatttgc ttttttatct aaacttaacc aaataaaagt ggctttacca
    16081 acgatgtttc tctcaggcac aaagccccag aaacgactgt cttcactgtt atcacggtta
    16141 tcacccatca caaaatagta tccttgtggc acaacccatt caccttctgc ttgcccttct
    16201 tgttggtaga aataaggtgc atagttaaat gccattgggt gatttaaaat ttgatgagtt
    16261 acattacctt tttcagtgcg ttctacctgc atttcaccgt gatagaaaaa ctcaggattt
    16321 ggcttgcctt cgctgtattc aaataccttt gtttcgccat tggctttagt aaccgttaat
    16381 tgctgagttg caaaatcata tttaatttta tcaccaccta ctgccaccac acgcttaata
    16441 taatcaacgt gtggttgtgc cggtgctttg aatacaatca catcgcctcg ttctggtttg
    16501 cccgtttcaa tcaaggtgtt ttgccagata ggatctttaa tgccgtagct gaatttattt
    16561 acgactaaaa aatcgcctac acgcaaggtt ggctccattg aaccacttgg aatttgaaac
    16621 ggttcaaata agaacgagcg aagtactgta acaaagaata aaatgccgaa aagtgaggca
    16681 aaaaattcgc ctactgcgga tttaggttga atttccgcta tttcttcagc ggttaattct
    16741 ctgcctaaac gtttttgttg tagaacaatc gcctgtttac gtcttgggtc agcactgaat
    16801 ttgtaaaacg cccaaaatcc accacaaata ataaccaagg caactaaaat aatggaaata
    16861 gtatttggca gttgcattga atcaagccat tgccaaatac cataaataat acctagaaaa
    16921 ataattggca taaattgtgc cactgaatgc tcctttttat tcaaaattgc aaagttttct
    16981 caaaatatga ccgcttgcaa gcggtcgtat tttacacgaa tattgacaat taatccttac
    17041 ccacatgaag aattgccaaa aatgcttctt gtggcacttc tacgttaccg agagatttca
    17101 ttcgtttttt accctctttt tgtttttgta gcagtttctt cttacgactt acatcaccac
    17161 cgtaacattt tgctaatacg tttttacgca actgttttac cgttgagcga gcaatgacgt
    17221 ggttaccaat tgccgcttga atagcgatgt caaattgttg gcgtggaata agttcacgca
    17281 ttttttccac taattcacgt ccacgatacg ccgcatttgc acggtgaacg ataatcgcca
    17341 acgcatcaac acgctcgccg ttaatcataa tatccacacg caccatatca gccgtttgga
    17401 aacgtttaaa accgtaatcg agtgaagcat aaccacgaga ggttgatttt aagcggtcga
    17461 agaagtctaa cactacttcg cccattggga tttcgtaagt taaggcgatt tggttaccgt
    17521 gatagaccat attggtctgt acgccacgtt tttccacaca aagggtaatc acgttgccta
    17581 ggaattcttg tggcacaagc atattacatt ctgcaatcgg ctcacgaatt tctgcaatat
    17641 tgttgattgc tgggagtttc gatgggctat ccacatagat cgtttcgcca ctggtttgca
    17701 ccacttcgta aactacggtc ggagcggtag taatcaaatc aagatcgtat tcacgctcta
    17761 aacgctcttg aatgatctcc atatgcaaca agcctaagaa gccacaccgg aagccaaagc
    17821 ccaacgcact tgaagtttct ggctcataga acaatgacgc atcatttagg cttaatttgc
    17881 ctaaagcatc acggaaggct tcgtagtcgt ctgaactaat cggaaataac cctgcataaa
    17941 cctgtggttt tactttctta aagcccggta atacttcagt tgctgggttg tggtggtggg
    18001 ttaatgtatc gcccactggt gcccctaaaa tatctttaat cgcacaaact acccaaccta
    18061 cttcacctgt ttttagctcg gtagtatcca cttgttttgg cgtgaaaata ccaaggcgat
    18121 ctacattgta agactgccct gtactcatca ctttaatttt atcgcctttt ttcagcacgc
    18181 catttttcac acgcactaaa gacacaacgc ctaaatagtt atcgaaccaa gagtcgataa
    18241 ttaacgcttg taatggtgct tccgcatcgc cttctggtgc aggcattttc ttcacgattt
    18301 cttctaaaac atcttcaatg cctaaacctg ttttagctga acagcgtact gcatcaatgg
    18361 catcaatgcc gacaatatct tcaatctctt ctgccacacg ctcaggctcg gctgctggta
    18421 aatcgatttt atttaaaatt ggcacaacct ctaaatctat ctcaattgcg gtataacaat
    18481 tcgccaacgt ctgtgcttcc accccctgcc ctgcatcaac cactaataaa gcaccttcac
    18541 aagcagcaag cgagcgagaa acttcatatg agaaatccac gtgccctggc gtatcaataa
    18601 agttgagctg ataaatttcg ccatctttcg ctttatagtt taaggttaca ctttgtgctt
    18661 taatagtaat tccacgctca cgctcaagat ccattgaatc taatacttga gcctccattt
    18721 cacgatctga taagccacca caagtttgaa ttaagcggtc tgataaagtc gatttgccgt
    18781 gatcgatatg agcgataatc gaaaagttac gaatattttg cattttcatc gggcagttaa
    18841 tgtcctgttt tgttatttgt ttatcaaaaa ttaatcgggg aattgtactt gaaatacaag
    18901 gttttgcaaa gaaaacaaat agcaataagc ggttattttt tcctaaaaat gtgcaaattt
    18961 cttgagaaaa atg
//
LOCUS       DANLXQ010000033        18323 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.033, whole genome shotgun sequence.
ACCESSION   DANLXQ010000033 DANLXQ010000000
VERSION     DANLXQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18323
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.033"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(262..4248)
                     /gene="hrpA"
                     /locus_tag="PXD94_000884"
     CDS             complement(262..4248)
                     /gene="hrpA"
                     /locus_tag="PXD94_000884"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL6285770.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQHDSYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDQDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARISRYSYRTKVQRLPIEPISQASAN
                     QRKGRCGRTSEGICIRLYSEEDFNSRPQFTDPEILRTNLAAVILQMTSLGLSDIANFP
                     FVDAPDSRQIQDGIRLLEELQAIIPNEKIPSTACGGGLGRGEIRSNDGTPPLPTSPRK
                     RGDGFRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSIQDPRERPQEK
                     QQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDYLNYNRVREWQ
                     DIYHQLRLAVREMGLKINSEPASYQPIHTALLAGLLSHIGLKDNEKMHYLGARNAHFF
                     IFPNSALFKKQPKWVMAAELVETTKLWGRMVARIEPEWVEPLAVHLTKSSYSEPHWAK
                     SKGAVMASEKVSLYGIPIVAQRSVNYGSIDPTVSREIFIRSALVEGECHNNYKFFKEN
                     HRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWWKQASKKDPEL
                     LNFEKSFLINDNANLASELDFPNFWHQGDLKLKLSYQFEVGKDHDGVTVHIPLPLLNQ
                     VEPEGFDWQIPGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAEPFKKPLLDNL
                     SHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDELKFALKDEVQ
                     NTLSNIADDGIEQSGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQTAVGVKLFETE
                     FEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLELIDDCIACAV
                     DQLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYELNKRLKGKLD
                     FTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKLLVDSNTDRAK
                     MLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLGTKYPISDKRI
                     LNVINEIK"
     gene            complement(4352..4804)
                     /locus_tag="PXD94_000885"
     CDS             complement(4352..4804)
                     /locus_tag="PXD94_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL6285771.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQNSAVTISAAVLLIMQQTLLSK
                     FIPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            complement(4867..5136)
                     /locus_tag="PXD94_000886"
     CDS             complement(4867..5136)
                     /locus_tag="PXD94_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285772.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            complement(5234..5830)
                     /gene="grpE"
                     /locus_tag="PXD94_000887"
     CDS             complement(5234..5830)
                     /gene="grpE"
                     /locus_tag="PXD94_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL6285773.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            6052..7146
                     /locus_tag="PXD94_000888"
     CDS             6052..7146
                     /locus_tag="PXD94_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL6285774.1"
                     /translation="MKKLAGLFAAGLATVALTACNEEKAAEPKATAAEAKPQATNQTV
                     HLYTWTEYVPEGLLDEFTKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYF
                     VSKMAKEGMLAELDHAQLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYK
                     GETFTSWGDLWKPEFAGKVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKL
                     RPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLA
                     IPANAKNKENAHKLINYLLSAKVAEKLTLAIGYPTSNVEALKVLPKEITEDTAIYPSA
                     EVLQKSQWQDDVGDAIELYEKYYQELKAAK"
     gene            7282..8673
                     /locus_tag="PXD94_000889"
     CDS             7282..8673
                     /locus_tag="PXD94_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL6285775.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVSAIAVSNSIYFPLFLFVSGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNLIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGSQVVA
                     SHQAALQTSSLFFMIPMSFGIATTIMIGQTLGQKNVEGAKILSYHALITATALAIIAA
                     IIIILLKNFIPFAFTNNPISIAITAHLLIFAAVYQIPDAIQAVANGILRGYKHTQPIL
                     YVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFWLVFCIGLSLAGGLLLWQMRKIQAV
                     PKAQLIAKLERIK"
     gene            8732..12256
                     /gene="recB"
                     /locus_tag="PXD94_000890"
     CDS             8732..12256
                     /gene="recB"
                     /locus_tag="PXD94_000890"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL6285776.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQHNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALSQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAESASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(12298..12780)
                     /gene="greB"
                     /locus_tag="PXD94_000891"
     CDS             complement(12298..12780)
                     /gene="greB"
                     /locus_tag="PXD94_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL6285777.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(12831..14111)
                     /gene="pepB"
                     /locus_tag="PXD94_000892"
     CDS             complement(12831..14111)
                     /gene="pepB"
                     /locus_tag="PXD94_000892"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL6285778.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLESCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFADIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            complement(14250..15071)
                     /locus_tag="PXD94_000893"
     CDS             complement(14250..15071)
                     /locus_tag="PXD94_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL6285779.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWQVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            15219..16162
                     /locus_tag="PXD94_000894"
                     /pseudo
     CDS             15219..16162
                     /locus_tag="PXD94_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(16275..17438)
                     /locus_tag="PXD94_000895"
     CDS             complement(16275..17438)
                     /locus_tag="PXD94_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL6285780.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(17504..18319)
                     /locus_tag="PXD94_000896"
     CDS             complement(17504..18319)
                     /locus_tag="PXD94_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL6285781.1"
                     /translation="MTLPFRAIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFA
                     TGRNHPDVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDD
                     QNVCLNSYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEA
                     LVPIEHYIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGD
                     GMNDVEMLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
BASE COUNT         4970 a         3817 c         3963 g         5573 t
ORIGIN      
        1 ttattttata cataatttta cctccaaaaa ataagcttat tttacgtagt taatttttta
       61 ttacaaataa taataatcta gttaatgata aagatcaaac tttctgatat atttagtttt
      121 ctaattttta tcttgagatt tttttaatca aaaaaatgta gcaggtttgc ttctgaagcg
      181 agaggaatag gtattcgaga aaacaagcgg tcagagttgc aaaatttttt acttatctga
      241 ccgcttgttg tatagggaat attactttat ttcattgatc acattcaaaa tccgcttatc
      301 cgaaatcggg tattttgtgc cgagctgttg agcgaacaga ctgacccgca gttcttcgat
      361 catatatcga atttccagta cctcgtccgg caccgctttg gattttggca gtttggcaag
      421 gagctgttta tatgcctctt gcactgcttc cacccgtagc attttggctc ggtcggtgtt
      481 gctgtccacc aacagtttat ccaaacggcg ttcgatagcg gtcaaataac ggtataaatc
      541 tgcaaggcgg gcgtagccgg ttttggtgac aaagtcgggg taaatcagcc cttcgatttg
      601 ggttttgatg tcgcttaacg caaacgccat cgtaaaatcg agcttgcctt ttaaccgctt
      661 gtttagctcg taggccagtg tgaggcactt ttcaacctgt ttggcaattt ctacggtttt
      721 atcgtttaaa tttgcccgca caaattcgtg cagttgggtg aatttttcct ccgtccagac
      781 aaacccgccg aactcttcta ccagctgatc taccgcacag gcaatgcagt cgtcaatcag
      841 ctccaatact ttgccgaacg gggcaaaata taagcccagc ttggctttgt tcggcagttt
      901 ttcgtgtaga tatttaatcg gcgatggcac atttagcaac agcagacggc gaagtcctgc
      961 ctgcattgca cgagcctgct caaattccgt ttcaaacagt ttcaccccca cagccgtttg
     1021 ttcatcgaca atcgcagggt aggctttcac gctgaaatgg gcttttttct gctcatagaa
     1081 ctgtggcaat tcggcaaaat tccaaatatg cacaccactt tgctcaatgc cgtcatcggc
     1141 aatgttggaa agggtgttct gcacctcatc tttcaaggca aatttcagct catcaaggtt
     1201 ttcgctctct tgcagtttct tgcctttatc atcaatcaca cggaaagtca tccgcaaatg
     1261 tggcggcagt tggcttaaat cccactgttc aggctcgacc aatacacccg tcattcggcg
     1321 tagttcgtgg cttagattgt ccagcaacgg ttttttaaac ggctcggctc gccctaaaaa
     1381 ggcgtccgca taattcggcg ccggcacaaa atttcgccgt agcgatttcg gtaaagattt
     1441 aatcagcgag acgatcagct cgtggcgtag ccccggaatt tgccaatcga aaccttcggg
     1501 ttccacttgg ttaagcagcg gtagcggaat atgcaccgtc acgccatcgt gatcttttcc
     1561 gacctcaaac tgatagctca gtttgagttt taaatcgcct tgatgccaga aattcgggaa
     1621 atccagctcg ctggcaaggt tggcgttgtc gttgatcagg aacgattttt caaagttcag
     1681 cagttcagga tcttttttgc ttgcctgttt ccaccaagta tcaaaatgct tgctcgacac
     1741 aacttcggtg ccaatccgct gatcgtaaaa ctcaaacagc acctgctcgt ccaccaaaat
     1801 atcacggcgg cgggatttgt gttccaatgc ctcaacttca cggatcaagc ggtgattttc
     1861 tttgaaaaat ttgtaattgt tgtgacattc gccttccacc aacgccgagc ggataaaaat
     1921 ctcacggctg acggttgggt ctatcgagcc ataattcacc gagcgttgtg ccacaatcgg
     1981 aatgccgtag agcgacactt tttccgatgc cattaccgct ccttttgact tcgcccagtg
     2041 tggttcgctg taactggatt tagtgaggtg tactgccaac ggttccaccc attccggctc
     2101 aatgcgagcg accattcgtc cccaaagttt ggtagtttcc accagctctg cggccattac
     2161 ccatttcggc tgttttttga aaagtgccga gttcgggaaa atgaaaaagt gggcatttct
     2221 tgctcctaaa tagtgcattt tttcattatc tttcaagccg atatgcgaca acaagccggc
     2281 gagcagggcg gtgtgaatcg gctgataact tgccggctca ctgttaattt ttaagcccat
     2341 ttctctaact gcaaggcgga gttggtggta gatatcctgc cactcccgca cacggttgta
     2401 gttcaaataa tccttacggc agagattacg gaactggttt ttcgacagtt ctttttgttg
     2461 ggcttggata aagtgccaca aattcacaaa ggctaagaaa tcgctctctt tatcggcaaa
     2521 acggcggtgt ttatcgtctg ccgactgctg tttttcctgc ggacgctcac gagggtcttg
     2581 gattgagaga gctgacacaa tcatcatcac ttcgtgcaac gaaccgtttt tcgccgcctc
     2641 gatcaccatt ctgcctaagc gaggatcgac tggcagttgg gcgagttggc gaccgattgg
     2701 ggttaatcta aacccatccc cccgtttacg gggggaggtt gggagggggg gagtgccatc
     2761 attagaacgg atttcccccc tccctagccc tcccccgcaa gcggtggagg ggattttttc
     2821 atttggaata atcgcctgta attcttccaa caaccgaatc ccgtcctgaa tttgtcggct
     2881 gtcaggggca tcaacaaacg ggaaattggc aatatcactc aatccaagcg aagtcatttg
     2941 taaaataacc gctgcaaggt tagttcgtag aatttcagga tcggtaaatt gtggacggct
     3001 gttgaaatcc tcttctgaat agaggcggat acagatccct tccgaggttc gcccacaacg
     3061 ccctttccgc tggttggcgg aggcttgcga aatcggctca atcggcaagc gttgcacttt
     3121 ggtgcggtag ctataacgag aaatacgagc cgtgccggtg tcgatcacat atttaatatt
     3181 cggaatagtg agggaggttt ctgccacgtt ggtcgccaga ataattcggt ttagcccacc
     3241 gcttggctgg aaaatccgtt gttgttcggc agccgataag cgggcgtaaa ggggtaaaat
     3301 ttcgctatgt cgcagttcct gcttttgcaa ggcttcggca gtgtcacgaa tttcccgttc
     3361 accgttcata aaaatcagaa tatcgccacg cccttcggct tgcagttcgt ccaccgcatt
     3421 tagaatacct tgcagttgat cttgatcttc cactatcggg cgataacgca cttccaccgg
     3481 gaatgtccga ccagagactt caataatcgg ggcgttgttg aaatgtttgg aaaaacgttc
     3541 tacatcaata gttgccgagg tgatgatcac tttcagatcg gggcgtttgt gcagaatttg
     3601 tttcagataa cccaaaataa aatcgttgtt cagcgaacgc tcgtgagctt cgtcaataat
     3661 cagcgtatcg tactgattta aatagctgtc gtgctggatt tccgccagca gaataccgtc
     3721 cgtcattagc ttgattaagc tgttttcact gacttgatcg ttaaagcgga ctttgtaacc
     3781 gaccgtctcg cccagttccg atttcagctc ttcggcaatg cgagtcgcca ccgaacgtgc
     3841 ggcaattcgg cggggctggg tatgcccaat caagcccttc accccacgcc cgagttctaa
     3901 gcacattttc ggtaactggg tggttttgcc cgaaccggtt tcccctgcga tcaccaccac
     3961 ctgattttcg ctaatcagtt ttaaaatttc ctcacggcga gcggaaacag gcagatcagg
     4021 gtagtcaatt ttcaggtttt gggcgtgctg agttcgtgcg aaaaaacgtg cctgacaagc
     4081 ggtgagatct tgcgaaattt ctgccaaaac tgaccgcttg tgatcgatgt ttttaatatt
     4141 ttccgcccct tttaagcgac cgagcaggcg gcggaaatcg gcggtggtga tattttccag
     4201 cttcacaagc ggtggtttct tatgattttt ttgcaaaggt gtttccattt aatttctctt
     4261 ttgcattaag accttgtgat ttattgtagg gtgggcgtaa gcccacgtaa cagaataggg
     4321 attttatgcg tggacttacg tccaccctac attaggtttt accaacaatc agtgacaaaa
     4381 tccctgccgc aatcaacggc ccgaccggta cgccttttaa gaacgccacg ccgatcaccg
     4441 tgccgattaa aagcccagtt accaacaccg gctggctgcc cattaaattc acgcccctgc
     4501 cacctagcca cgctaccagc acgccagcaa ttattgagag tgccattttc cagttcaata
     4561 gttgggcaag ttcgggaagc gtaattcgcc ctgaaaccaa ggggctaagt acgccgatgg
     4621 ttaaaatgat aatcccgatt tttaagccgt attgatcgac aaaggggata aatttagaaa
     4681 gtaaggtttg ttgcataatc agcaatacgg cggctgaaat agtaaccgca ctgttttggc
     4741 tgatcagccc taaaataatc agcaccacaa gtagaagtgc cactgcgtta aattgtaaag
     4801 tcataggatt aaaacgtaaa aacccttact tcatagaagt aagggagtgg ttaattaaac
     4861 gttgagctag ttaataaatt tcattattgc cattaaaaag ccaagaattg tgatatttaa
     4921 ggtaattgct ttaaatatcg tggtattgat ttcatttttt agcttttcaa gttcaagttg
     4981 caaatcagct tttgttgcta gtgggctttg actttgctct aaggcttcat cgagagcatt
     5041 agcaaatgcc tcagccattt caggtgtttg attggcttct tgcaagcgtt ttacaaaacg
     5101 taatttgtca aatcgtaggc tcatagctgc atccatttca attatcctct tgtggttaag
     5161 ttataaacta atttagcata atcacaagca ggaattttag gagattttgt gatcttcgtc
     5221 gaaaatcgac aatttagttt gccaccatca ccattgccgg gcgaatcact cgtccgtgaa
     5281 gcgtgtagcc tttttgtagc actgtgctga catggttggc ttcaattcct tcggcaggtt
     5341 gcattgaaat agcttcgtgc aattcagggt taaatgcctc gcctacttcg ccaattgcca
     5401 ccacaccaaa tttagccagt gtattgatga attctttatg agtcatttca acgccgtcaa
     5461 ctaacgcttg agtggtttca tcggtcacgg ctttatcaag ggcatctaat ccacgttcaa
     5521 ggttatcaac cactgttaac aattctttgg agaatttttc taaagcgaat ttgtgagctt
     5581 tttcaacatc ttgctctgca cggcggcgga tattttgaat ttctgcttga gccctaagct
     5641 gaatatcttg ctcacgtttg tcggcttctg caatataggc ttctaactcg gcaattcgag
     5701 cttgagcgag agctaacggt tcttcttgag gtgtattttg ttcttccgct tgagtttctt
     5761 cagttacaac ttcaggttga atttcttctt gttttacttc ttcttgtaag tgttcatttt
     5821 gagtggtcat tttttatcct ttaaaagact aatctggaaa tgtaaatctt ccttaatata
     5881 gcgataaatt aagggatttc aagcatatct ctttcttttt gcgaaaataa ttcacttttt
     5941 aatcagaaaa ggcttgcaaa atacgggggg gggcttaaaa tgccctagtc ttttccgccc
     6001 ttaggcaagg ctatattttt taatcacttt tctttaacgg agaaaactta aatgaaaaaa
     6061 ttagcgggtt tatttgcagc aggtttagca acagttgcat taacagcgtg taatgaagaa
     6121 aaagcggcag agccaaaagc aactgcggct gaagcaaaac ctcaagcaac aaatcaaaca
     6181 gttcaccttt atacttggac tgaatatgtg cctgaaggtt tgttagatga atttacgaaa
     6241 gaaaccggca ttaaagttga agtttcaagt cttgaatcta acgaaacaat gtacgctaaa
     6301 ttaaaattac aaggcaaaga tggcggttat gatgtaattg ctccatcaaa ctactttgta
     6361 tctaaaatgg cgaaagaagg aatgttagcg gaattagatc acgcacaact tccggttatt
     6421 aaagaattaa accaagattg gttaaataaa ccttacgatc aaggaaacaa atattcatta
     6481 cctcaattat taggtgcgcc gggtattgca tttaatacgg cagattacaa aggtgaaaca
     6541 ttcacttctt ggggtgattt gtggaaacct gaatttgcag gtaaagtaca attattagat
     6601 gatgctcgtg aagtattcaa cattgcgtta ttaaaattag gtaaaaaccc aaatacaact
     6661 aatccggatg aaatcaaaga agcctttgaa gagttgaaaa aattacgtcc aaatgtgctt
     6721 tcatttacct ctgataaccc ggcaaactca tttattgctg gtgaggtttc tgtaggtcag
     6781 ttatggaacg gttctgtacg tattgcgaag aaagaaaacg caccggttga tatggtattc
     6841 ccgaaagaag gtcctgtgct ttgggtcgat actttagcca ttccggcaaa tgcgaaaaac
     6901 aaagaaaatg cacataaatt aatcaactac ttattaagtg caaaagtagc tgaaaaatta
     6961 acgttagcga ttggttaccc aacttcaaac gtagaagcat taaaagtatt accgaaagaa
     7021 attactgaag atacagcaat ttacccatct gcagaggttt tacaaaaatc tcaatggcaa
     7081 gatgatgttg gtgatgcaat tgagctttac gaaaaatact accaagagtt aaaagcagca
     7141 aaataatagc tgattaattc tgaagcctct agggagcaat ctttagaggc tttctctttt
     7201 gagttgcaaa aaatttggca aaaacgaccg ctttctaagg tacaatcata aaaacatact
     7261 tagattttga gagattaaac catgaactta agatggcaag aatatcctga aaatgcccgc
     7321 aaattattta aactatccct acccattttt atttctcagc tttcagcttc cggaatgggg
     7381 ctggcggata ttgtaatggc tggcttggtg agtgatgatg atgtctcggc gattgcggta
     7441 agtaattcga tttactttcc gctctttctg tttgtgtcag ggttgctgaa tgcaattacc
     7501 ccaaccgttt cttacttaaa cggctcgagc aatcgccatt taattgctaa ccaagtaagg
     7561 cagggctatt ggattgtgtt tgcaatgtct attccattaa tggcaatttt cctaaacagc
     7621 catctgattt tagacttaat gcaaacaccg gctgagtttt caatcaaatc gcaacaatat
     7681 ttagcagtaa tggcagttgg gcttattcct gctttgctgg cggtgagttt acgttgtatg
     7741 aacgatggat tatctaaccc taaacctgcg atgcggatta cctttttcgg tttattgctg
     7801 aacattccgc taaactatat tttcattttc ggcaaatttg gcttgcctga aatgggagcg
     7861 gtaggctgtg gtgtagcaac cgcaattgtg aacttgatta tgttttttat gttgttgcat
     7921 tattgctaca ccaattctgc ccaaaaagat attcgtttgt ttgagaaatg gattgatgct
     7981 ccaagcggtc aaattttgct aaaactttgc aaattagggc tgccgattgc ctttgcgatt
     8041 tttaccgaag tgatgctgtt ttcgacctca tcgctgtttc tgtcaccact tggttcacag
     8101 gtggtggcta gccaccaagc tgcattgcaa accagctcgc tattttttat gattccgatg
     8161 tcgtttggca ttgcaaccac cattatgatc ggacaaacac ttgggcagaa aaatgtggag
     8221 ggggcgaaaa tcttaagtta ccacgcactg attaccgcaa ccgctttagc tattattgcc
     8281 gccatcatta ttattttgtt gaaaaatttt atcccttttg cgtttaccaa caacccgatt
     8341 tcgattgcga ttaccgctca tttattgatt tttgcggcgg tttatcaaat accggatgcc
     8401 attcaagcgg tagcaaacgg cattctgcgt ggttataaac atacccaacc gattttatac
     8461 gttactctgt tttgttattg gggggtgagt atgccgctgg gctacatttt ggcaagaacc
     8521 gattggattg tacaaccaat ggctgctcaa ggtttttggt tagtcttttg tatcggctta
     8581 agtttggctg gtggcttgtt attatggcaa atgcgaaaaa tacaagcggt gccaaaagca
     8641 caattaattg caaaattaga acggattaaa taacagattc tctgctatgg aacatagcag
     8701 tgctttttac ttttagaaga taactttgct gatgaaaaac ctaaatccta tccaactacc
     8761 tttgaataaa tccgttttaa tcgaagcgtc ggcaggtaca gggaaaacct ttaccattgc
     8821 caacctgtat ttgcgtttat tgctcggcat tggttgcaac ccgttaacgg tggaacaaat
     8881 tttggtggtg acatttacca aagcagcaac cgaagagttg aaaaaccgta tccgtaaaaa
     8941 tattcagcaa tgtgccgact ttttaaaaga tcaagctgat ggtttggaag tggaaagtac
     9001 aaaatcctat cgaaacaacc ttgatttttt ggcacagatt tatccgctca ttccgaatat
     9061 tcacgaagca ttgttacgtt taagtattgc cgaacgggaa attgataccg ccagcgtgtt
     9121 taccattcac ggcttttgtc aaaaaatgct ggttcagttt gcgtttgagt cgggcgtgcg
     9181 gtttgacttg gatttacagc caaaccaaag tgatctactt aaaaaattaa gtgaagaagt
     9241 ctggcgagag caattctatc cgcaagattt agccatcact tatgcggttg ccgaacaact
     9301 tggtacgccg gaatatgccc tcaatgcggt aaggcgttat ttatctactg aattacctga
     9361 accaaatgcc tctttaaatc aagatattgc cgagcatttg gctcaatatc agcagtttat
     9421 taatgaggtg aaatcctatt ggaaaacgca ttcagatgaa ctggttgaaa ctatccgcca
     9481 cgatttaaag cataaaatta cgctgcacgg aggttggtat cgagcgacca cttttgaagc
     9541 cggtgtggaa gtgatgaacc agtgggctaa ttcgcaacat aaccatttcc cggcagggct
     9601 ttatttattc actacagaga aaattgcggc tggcacgaaa aagagctgtg agccgataga
     9661 ttcccctcat cttgcaaaaa atcaggcttt tttgaccgct tacaaggaac gttttgaaaa
     9721 cagcttgtca gcaatgttgc agtatcaatt tttaattgct ctcaaagcaa aattggcaga
     9781 gtacaaacaa tcgcacccac aacgcagttt tgatgattta ctcacaatgc taaaccaagc
     9841 gttgaaaaat ctacacacgg gcgaagcctt gtctcagaaa attcgggcga tgtatccttt
     9901 tgcgatgatc gatgagttcc aagataccga tttaacccaa tatgaaattt tcagccaaat
     9961 ttttatggca gagaatgcag agaacggctt tattatgatt ggcgacccga aacagtcgat
    10021 ctataaattc cgtggggcgg atattttcac gtatttgcga gcagcaaagc aggctgatga
    10081 acaattcaca ttgggcaaaa actggcgttc gttgcctgaa gtggtgcaag cggtcaataa
    10141 tttgttttct tttgcaaatg ataactctcc gtttctttat gaggatattg gctttcaccc
    10201 agtggaggct gatagccaaa aaggtcagtt agttagtgag caagggcatt ttgtaggcta
    10261 catcacggaa aacagtaaat tagatgattt ggctgaaatg tgtgcttatc aaattcatca
    10321 acagcttgag gcgatggctc aaggcaaatt cggagttgaa aaaaacggcg aatttcaatc
    10381 ctttgctgca aaagatattg cgattttagt ccgaaaaggt tcacaagcta agcgaatcaa
    10441 aaaggcgttg gcaaaacgta atatcaaatc cgtctattta gctgaatcag cttctgtatt
    10501 tggttcggaa attgcaacag aattgtgttg gttattgcaa gccgcactga atccgtatca
    10561 ttacaaggca ttattatcgg cgttaggctc aaccttgtgg ggcttaacgg cggcagagat
    10621 ccaccgctat aaggaaaatg agcaactttg ggatctgaaa gtcaatcagt ttgtggaata
    10681 cagcaacatt tggaaagagc agggcgttct gccgatgttg cataaaatct acttggagca
    10741 gggcattatc gaacgtttga aaggtttgga aaatgcggat cgcttgatta cggatttatt
    10801 gcatttaacc gaaattttgc aggaaacctc gcaagcgtta gagtctgaag ctgaactgtt
    10861 gcggtggtat gaaaagcaag ttaattcgcc cgattttaat gacgagcaac aacttcggtt
    10921 ggaaagtgaa gaggagctaa tcaaaattgt gacgattcac ggctcgaaag gcttgcaata
    10981 tccaattgtt tggttacctt ttgtttcaca aaaaagtgag ggagacacgc agacagcatt
    11041 aagtttatat cgcaattctc aaggtgcgct ggattggtgt ttaggctcag aagaggcaag
    11101 tttttcgcta cagtatgagg cttatgcgga agatttacgt ttgctgtatg tggcattaac
    11161 ccgcgcggaa tctcagctta acttagtgtt gccgaaagag atttccggct ggtctccact
    11221 ccattacttg ttagcagacg gggaaatacc tacaggaaaa gtgcctgttt cagattattt
    11281 tagccaaaaa cggattgctg ctaatttggt tgaagtagcc ggggaattac ctttctcact
    11341 gtgggaagga gaacaatctt cagacgatta ccaagcaaaa acctttagtg gcaatatccg
    11401 tgtggttggt caaattacca gctttacttc actttatgcc caacacgagc gttttagtga
    11461 agggaaatca gaaagggtaa tggatttagc ccaagatcga gatgtacata tagaaaccat
    11521 tgaggcaggt tattcaagtg aaactgccgc tttctcgcca ttccaattcc cacacggtat
    11581 taaagtgggc acggtgttac atcacttttt tgagcattgc caatttaacg aacaaattga
    11641 tagagaggct gttgctaaag tatgcgagca attagggcta agcgaggaat ggattgaacc
    11701 aacagcatta tggtttgagc gtattctaac tacccctttg gcagaggcaa atttctgctt
    11761 aaaagcgatt gatgaaacca aacgattaaa cgagtggcaa ttctatttac gcttaaaaaa
    11821 tgataaagcc ttacatcaat taaatgcgtt attaaagcaa catagccctt tagcaaaaac
    11881 tttacctgaa ttgcaattac cgcaattaga aggttttgtg cgaggctttg tagattgtat
    11941 cgtgcaagtg gaggagaaat tctacttaat tgattacaaa tcgaatttct taggctatct
    12001 gccacaaaat tatgcgaaag aacacattca acgggaaatg ggcagacaac gctatgattt
    12061 gcaatatctg ctttacactc tcgccttaca ccgttatcta actgcccgtt taggtgaaaa
    12121 atacgattat gaacgagact tcggcggagt ggcttacttg ttcttaaggg caatgaacgg
    12181 cgaaaatgat aacggcgttt attttgacaa gccgactaag cagttgattg aagaaatgga
    12241 tcggttgttt gggtaaatat gtgtaggggc gaaaattttt cgccccttta tgggtaatta
    12301 actcgcttgc tcataccaaa tcttattgat ataaagcaac actttaccaa gcggagtttc
    12361 taccctaatt tcatcatcaa cctgtttgcc gattaaggct cgggcgacag gggagtcaat
    12421 cgaaatccag ttttttgctg ggtcgaactc atcgcaacca acaatacggt actgttttgc
    12481 ctcgccctct tcattttcta attccaccca agcgccgaaa aagactttgc cttcttgttt
    12541 agggtgataa tcaacaattt gtagcacctc tagccgctta gataaaaaac gcacacggcg
    12601 gtcgatttct ctcaaacggc gtttgccgta aatatattcg gcattttcgc ttcgatcacc
    12661 caatgcggca gcatcagaca ccgcttgagt cactttcggg cgttcttccc gccatagaaa
    12721 ttttaattcc tgatctaagg cttgccagcc tgaacgggta atgtaatttg atttagccat
    12781 agttatttaa ttgtttatgg agcaaatagt gatttgcccc tacgaagttt ctattttgaa
    12841 agcaataaat tcgctactgt tcttattccc agtccggttg ctcctgccgc ccataaatcg
    12901 ctcgccgttt tgcggaatgt ggcagaggcg tcaatatgta gccagttttg ctggtagttt
    12961 tctacaaagt aagataaaaa tgcggtggcg gtacttgccc cagcccccac cggcacagag
    13021 ccgatattgg cgatgtctgc aaaggaagaa gcaatttgcc cacggtgaaa ttcttcaaac
    13081 ggtaaacgcc agaacggttc attttccgtt ttggcagact taaatagtgc ttcggttagg
    13141 ttgtcgtcca ttgaaagcac cgagtggtag tcattgccga ctgcgacttt tgctgctcct
    13201 gtgagggttg cagcatcaat aataaattgt ggattttgct tgcacgcatc aattaaacca
    13261 tcggcaagca ctaaacgccc ttcagcatcg gtgtttaaaa cttcaacagt tacgccattg
    13321 cggtaagtga taatatcgcc taatttgaac gcattgtggc tgaccatatt ttccgcacag
    13381 cagaggtaga gtttcactcg tttattcaag cctcgagcaa tggcaaaagc aagtgagccg
    13441 gttaaaagtg ccgctccgcc catatcggtt cgcattgtgc tcatactatc gcttggtttt
    13501 agactataac cgccggtgtc aaaggtaatg cctttgccga ccaagcaagc cagtacctgt
    13561 gcgttttcat cgttggttgg gttgaagtct aatactaaga atgcaggcgg attggcagag
    13621 ccttttccaa ctgtccaaat gccgtgatag ccttgttctt gtaatgcttc gccacgaatg
    13681 atctggtaag aaaccgtatt cggttttgcc attttttgga taaactcgac cgcttgttcg
    13741 gctaattttt caggggttaa tttatctgat ggcttattaa ttaattcacg cacgaaagtg
    13801 gcacatttta cacggttatc aaattcggct tgctgttcgt ctaagtctgg gtattgtacc
    13861 gagccttgat tttttaccga atgaaatcct tgatgaaacg cccagcagga ttctaaatcc
    13921 caactatcgc cggtcagctc cacggttgaa ataccttgat ttttaatttt gcgggcggct
    13981 ttttgaatag tggctaaatc gttgttcgga ttatgtacgg taaaccccgc atcatttgcg
    14041 gaaagaatag catttttacc ccaaatttca ggggcgtttt gagtagaaag tgaaattttc
    14101 attgctgtca ttatcggttt ccttagaaaa tggattttgc tttatcctaa caaacttttg
    14161 caaagtttta tacaaaaatg accgcttgca gagcatagcc ttgacaagcg gtcattgttt
    14221 cgtgttttta caacctgatt aaggacggat tatttgccgt cccaagcttt aattgcattg
    14281 tggcggattt taacacggct ttcagcattg cctttgtagt aatctttatc taaaccgcct
    14341 tcccagtcgc catagttcgg gttcggtaac ataatgaatt ttttaccgaa ttgttggctg
    14401 ttttcggcta cgaatgcacg gcgttccgcg tttgatttac ggtaagtcgc atcgccgaaa
    14461 tcgtttaagt tgtcgccaat atagagcacg atttcatagc cttgtttttc gatttcttca
    14521 aagcgagggg ttttgttcga tttgtctttt ttcaacaaga gtgtttgttc gctcacacca
    14581 ctaaagccta atttgttcat atcatcaata gtaccggctt tttcactgct gtctttacgg
    14641 tttgaaacat agaacatagt tcctttgtgg ctattaacgt agttgttaaa ttccactgca
    14701 ccggcaatcg ctccggtttg gcgggcattt acccaacgag tccagctttc accatcaaac
    14761 ggcttgtggt ttttaacctg ccagccggca taaaggctgt tgtccaccat agtttcatct
    14821 aaatctacta ccacggcttt tttcttgcct tttttcactt tggcttggtc gaatgccact
    14881 ttcgctgcat tgaatgcttg atgggcaaga gcttggtatt cgccggattg ttgcatccag
    14941 tttatcccta atgtagcttg ctcttgcaac accatttgat gatgctcatc gctgttttta
    15001 tgcgtgtgcg aacaagcagt taatacaaat aaagctgtga ggctcatagc agaaagtttg
    15061 atgtttttca tacgtatatc cttagtttta ttgttgataa acagagcgat tatacctaaa
    15121 aagagttgct tatggctaat agtttattgc tcctattcac ttttaaagca aactaaggta
    15181 gcctagtaac ctttttataa caattacaca gaggctatat gcaaacatta ttaaaattta
    15241 cacaatttat cagcaaaacc ttcgccattt gggtggtgtt ttttgctttt atcgcagcac
    15301 aatttccgga aacctttaag caattcctgc cttggattcc ttatctgtta gggattgtga
    15361 tgttgggaat ggggttaacc ttaactttca aagattttgc cgaggtaacg aaaaatccga
    15421 aatcagtgat tatcggcgtg atcgctcaat tcgtggtatg ccgagtgtag cttatggatt
    15481 ggcaaaagca tttaacttac ccgcagattt agccattggt gtgattttag ttggttgttg
    15541 cccgggcggt acttcttcaa acgtgatgac ctatttggct cgtggcaaca cagcgttatc
    15601 ggtagcttgt accactattt caactttact tgccccagtg ctcacgccgg caattttcta
    15661 tttatttgcc agccaatggt tagaaattaa tgcaacggca atgtttattt cggtgttgca
    15721 aatggtgcta ttgccaattt ttgtgggcgt ggtgattcgt actattttca aacaaaaagt
    15781 agagcaattt agccaaacaa tgccattaat ttcagtgatt gcaatcgtat taattgtcac
    15841 ggcggtggtg tcagttagcc gagatcgtat tattgaatcc ggtttattaa tttttgcggt
    15901 agtagcatta cacaacggtt taggttattt aatcggttat ttggcgagcc gtttattaaa
    15961 attaccgatt gcagacagca aagcggtggc gattgaagtg gggatgcaaa actcaggatt
    16021 gggggcagca cttgccgcat tacactttaa agccaaccct atcattgcgg tgccaagtgc
    16081 ggtatttagc ttctggcaca atatttcagg cccgattttg gcgatgattt tcgctaaaat
    16141 gaaaaacgat aacgaaaaat aaaattcgtt ttaatacaga cgttttaatg ttttaataaa
    16201 gaatagacaa acggttaaat ttttctaatt ttttgcaaaa tgttcagaaa atttaaccgc
    16261 ttgttgttta gaatttagaa cattcttggt ccacgtgcag tgctgatttt ttcatcatcc
    16321 gctctttggg taacaatcac ttgatcaccc tcatttaagc ctgctaatac ttcggtattt
    16381 tgcgtatccg aaatgccaat ttggattttt ctttcaaccg actgctcatt ttccagcacc
    16441 aacacataat tctctttacc acgttttttg attacgctgg tgggtacaac taaaacctgt
    16501 tcccgtttat caatagtaat ttgggcttga gtagtcattc cgatgcgtaa actgttatcg
    16561 gcattatcaa cggaaacatt agcgtagtaa taaaccgctt cggtattacc cgattgttcg
    16621 gtgtagttat tatcggtcag cgtggtgagt gccggatcaa ctgaatcaat ggtagcacta
    16681 tattctcgat ttggctcagc aagggtggaa aacttaactt tttgcccgac tttaacctgt
    16741 gcaatatcgc cctccgaaat ttcaagttta atcagcatct tagataaatc tgctacctgt
    16801 acaatagttg gcgaagtttg attggaattg accgtttgcc cgacagaaac cggtacagac
    16861 acaatcacgc cgtccattgg cgagataatt tgcgtataag ttaagttggt tttagcatcg
    16921 tttaccgaaa tttcagccga ttttaattgt gctttaatgt tatccaccgt ggctttggct
    16981 gaagctaagg tatttttagc cgtttcaaga tcgtttaaag aggcactatt tgcccgatag
    17041 agtttattta agcggttgta gttcgattga gctacttcaa gtgcaacctg tgcagaggtg
    17101 agctgtgctt tataagaaga aagccgggct tgagcggtgc ttaaggtgtt gctctggttg
    17161 ctggaatcaa tatctgcaat taaatctcct tgcttcaccg attgcccgag ctcgacatag
    17221 agtttttgga ttttgcccga aacttgagcc cccacttcgg tgcgtttatt cgctcgaacc
    17281 gaaccggttg ccagcacatt tttgttaatt tctgcccgtg tgacagcttg ggtaatgtaa
    17341 tggatttgcg gtttattggt ttgttgctga taataaaaat agccgcctat tccgagagcg
    17401 gcaattagca caagagtggc taatgtttta cgtttcatac tgttccttga tgatatacaa
    17461 gcggtcaaat ttagacaaaa atttgcaaaa aagttgctct caactaaaca atgccaaatg
    17521 tggctcgaag atagcttgcc accgcttcat ctttgttaaa gccaatcact tcattattcg
    17581 gtaatacggc ttttaaacgt ggatcggcat tacccattaa acaacctttg ccggctaaac
    17641 ttagcatttc cacatcattc ataccgtcac caaaagcgat acaatcggct aaggtgtagc
    17701 cccgtaattt gaccaattcg gcgagagcat tggctttaca cactcctttt gccatcactt
    17761 caaggcaaag tagggcagag taggtcattt gcacacggtc gccataggta tcccgaatat
    17821 aatgttcaat cggtactaaa gcttcagcag ttttgccgat aaaaaacact ttctcggtat
    17881 ttcgaccgtg atgatgagca aaatttacta cttgatagct aaatcctgaa tcttgatgga
    17941 atttttttaa ctctgcaatc tctttattga taaaccattc atcgccttga tagctgttta
    18001 agcacacatt ttggtcgtca aattcaatgt tcattaattc aaaggcaaga tcttccggta
    18061 aataatggtt gagaattttt ttaccctcta aattgttggc tcttgccccg tttgaagtga
    18121 ctaacattgc atttttaacg tttactttcc caataatctg agacacatct ggatgattgc
    18181 gaccggtggc gaaaaaaata tcaacacctt tttttgatag ttgttctaat gtatcaatag
    18241 tgaaatcacc aattttatga ttggcattta atagtgtgcc atcaagatcg gaaataattg
    18301 cacgaaatgg gagagtcata atc
//
LOCUS       DANLXQ010000034        17980 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.034, whole genome shotgun sequence.
ACCESSION   DANLXQ010000034 DANLXQ010000000
VERSION     DANLXQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.034"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            71..1162
                     /gene="ulaG"
                     /locus_tag="PXD94_000897"
     CDS             71..1162
                     /gene="ulaG"
                     /locus_tag="PXD94_000897"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL6285782.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVAAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            1246..1992
                     /gene="ulaR"
                     /locus_tag="PXD94_000898"
     CDS             1246..1992
                     /gene="ulaR"
                     /locus_tag="PXD94_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL6285783.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILAPTFLQHTKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQALRNKGLDIILA"
     gene            2047..2907
                     /locus_tag="PXD94_000899"
     CDS             2047..2907
                     /locus_tag="PXD94_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL6285784.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQQGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            2904..3623
                     /locus_tag="PXD94_000900"
     CDS             2904..3623
                     /locus_tag="PXD94_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL6285785.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            3844..6321
                     /locus_tag="PXD94_000901"
     CDS             3844..6321
                     /locus_tag="PXD94_000901"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012311.1,HMM:TIGR02601.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL6285786.1"
                     /translation="MYLLEGNQKVKNRNYFVAIATILALSPEVNATELSDQLASRTRY
                     YPSATDSYYSYYKYAPIYSGYYTSNSRNGYSSWYYPGSNAGVTTRTVSTTVNGITVTK
                     VYTTHNGRTTVRTTYAPAVNKPIVNKPTPIANKPVVDTPTQPVIDANPIMDPPVQPIV
                     DNKPITDTQPNPITDNKPVIESPIDDTRPVIIRPTTPVNLHPEPVAVGVPNVGIRWND
                     RSRGYNIDDPNNKQNVKLTGEGVKVGILDSGFKNYLMQDDILKKFGNRAVIIETGRTA
                     PASATHGIEVAEMVGGGNSRNGVASKSTLLLADITKTTNAGTGLSATAPIYYDLWNRG
                     ARIFNQSYGIPKPLTYFNNNRNSSYYYLHQFDGNVLQFYKDKVKEGGLFVWAAGNNRG
                     DLDPSLQAGLPHYEADLEKGWISVVAVATRTNQGLGNFEWSNLLPYSQAGVAKNWTVT
                     AMGDYIFNLGGRNIIASGSSFAAPAVTGTAALVKQKYPWMDGNLIKQSILSTATDIGA
                     PGVDDVYGWGLLNIEKATNGPARFGKRLALADNVTVNIPNGSYEFSNNINGDAGLVKN
                     GQGELILSGASTFEGDTTLNEGAVTINGKKYVSKVNVSPSGTLVVNNTTLENGVNNEG
                     TVVNQGYSTIKRAYVASAQSTLVSDLGSNLNVKGEVDLNGSTLSLAAVKEGKAQYVTQ
                     KGLSMSAITSDSAIKGNFAAIETSGLLTATAEKTNENEVKATVSRKNVATALESSFSA
                     LDNQIEGNNGSTNATNDEFAQQAATLQNSIANINTQSVVLDSLSGQIYASAQALTFEN
                     SETVNKDLSNRLVMLGTLDNVGETAGV"
     gene            6331..7146
                     /locus_tag="PXD94_000902"
     CDS             6331..7146
                     /locus_tag="PXD94_000902"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6285787.1"
                     /translation="MYGNGTLKEIGFGEGKTNTYAGQIGFDKPVTDHVILGAALNYSK
                     AKVNFDRYGGSSKADGIGASLYARIGNKHKTPWYLQGRAGYGSVDSDVERHIILADNN
                     ASTAKINHRDRVFSAYVESGYDFKQGRFALTPFVGFSHDVVRRGAFSEQNSQFGLTAD
                     KATYKQTSGLIGLRTSLEVNLAGMKTTFQGYVNHQKAFNREDLSFKASYTGLQDAKFD
                     VKGIGLAKHKTWVGIGALAEVNKNTSLYVNYDLKLAKNSGHNNVVTAGIRINF"
     gene            complement(7233..8093)
                     /gene="pdxY"
                     /locus_tag="PXD94_000903"
     CDS             complement(7233..8093)
                     /gene="pdxY"
                     /locus_tag="PXD94_000903"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL6285788.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAVKIA"
     gene            8212..8287
                     /locus_tag="PXD94_000904"
     tRNA            8212..8287
                     /locus_tag="PXD94_000904"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8245..8247,aa:Thr,seq:tgt)
     gene            complement(8382..9335)
                     /gene="accA"
                     /locus_tag="PXD94_000905"
     CDS             complement(8382..9335)
                     /gene="accA"
                     /locus_tag="PXD94_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL6285789.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(9389..10813)
                     /locus_tag="PXD94_000906"
     CDS             complement(9389..10813)
                     /locus_tag="PXD94_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL6285790.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSVERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            complement(10815..11549)
                     /locus_tag="PXD94_000907"
     CDS             complement(10815..11549)
                     /locus_tag="PXD94_000907"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL6285791.1"
                     /translation="MLKIFRILIVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGA
                     LHKLVGLELITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFF
                     GLVYWATGNIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTG
                     AFHLAISAGVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEK
                     CHQIMLAKFEQLNQEVEQLENTAKNA"
     gene            11696..12193
                     /gene="tpx"
                     /locus_tag="PXD94_000908"
     CDS             11696..12193
                     /gene="tpx"
                     /locus_tag="PXD94_000908"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL6285792.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(12278..13903)
                     /gene="yidC"
                     /locus_tag="PXD94_000909"
     CDS             complement(12278..13903)
                     /gene="yidC"
                     /locus_tag="PXD94_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL6285793.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(14056..14481)
                     /locus_tag="PXD94_000910"
     CDS             complement(14056..14481)
                     /locus_tag="PXD94_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL6285794.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(14810..17902)
                     /locus_tag="PXD94_000911"
     CDS             complement(14810..17902)
                     /locus_tag="PXD94_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL6285795.1"
                     /translation="MIPRLSEVPQLDKATQSYLTELVNQHFSGEIASNYADRLSLATD
                     NSVYQQIPQAILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVD
                     LSRHMNQILELNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINT
                     DASGQGSLQYGKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHR
                     EIFNRCKTLRPKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICE
                     AKLNLLPIPKYRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWH
                     SVSDLLTEDPANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLT
                     SDVNSIEKIYAMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYN
                     LPYGMFGHVDAGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMR
                     SYYGERFFGETLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQ
                     IPIQVREEFKGAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLL
                     TEQNVSPDDLIFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACA
                     SQCPIKIDVPTFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTF
                     AEKAANKTIGMTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFI
                     VQDPFTSYYDAKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQAD
                     FLNRVAKLGLPMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELEN
                     CKKSANSDRLPWYLFAHCTESTALPSSMKEWQAIFTHFGETLLNENVGCCGMAGTFGH
                     ETKHVEMSKAIYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI
                     "
BASE COUNT         5120 a         3637 c         3934 g         5289 t
ORIGIN      
        1 aatttttgtg attgtttatg attggttttg attgcattgt tcttatttta gcgtaaatca
       61 ggagactatt atgagcaaaa ttcaagaaat cactcgtgaa agctggatcc tttcaacttt
      121 ccctgaatgg ggtacttggt tgaatgaaga aattgaagaa gaagttgtac cggaaggcaa
      181 ttttgcaatg tggtggctag gctgtgtcgg tgtgtggatt aaaaccccag ctggtgcgaa
      241 tatctgtatg gatttatggt gcggtcgtgg taaatccacc aagaaagtaa aagatatggt
      301 gcgtggtcat caaatggcaa atatggcagg ggtgcgtaag ctccagccta atttgcgggc
      361 tcaaccgatg gtgttagatc cgtttgcgat taacgaagtg gacttcattc tcgcttctca
      421 ctatcactca gaccatattg atgtaaacgt ggcagcggct atttgcaata acccgaaatt
      481 agaccacgtt aaatttgtcg gcccttggca ctgtgcagaa ttatggaaaa aatggggcgt
      541 gccggaagaa cgtattattg tggtaaaacc gggcgatgtc gtgaaattaa aagatgtaga
      601 aattcacgcc ctagattcat ttgaccgtac ctgtttagtg accttaccgg tagaaggtgc
      661 agaagaacgt ggcggagaac ttgcaggctt atgcccatca gatgaagaaa tgggacgcaa
      721 agcggtgaac tatgtgttca aaaccccggg cggtacgatt tatcacggtg cagattctca
      781 ctactcaatc caattcgcta aacacggtaa gcaatttgat attgacgtag cgctgaataa
      841 ctatggcgaa aacccggttg gtattgcgga taaaatgact tcagttgatt tattacgtat
      901 ggcggagtgt ttacgctgta aagtgattat tccggttcac catgatattt ggacaaactt
      961 tatggcaagt acgcaagaaa ttattgattt atggcgtatg cgtaaagatc gcttacaata
     1021 ccaattccac ccatttattt gggaagtggg cggcaaatat gtttacccac gtgataaaga
     1081 cttaattgaa tatcatcacc ctcgcggctt tgatgactgc ttcgagcaag agccaaatat
     1141 tcaatttaaa tccatgctat aggtgagcag tcgccactct cactggagag tggcgtttta
     1201 gcaagttcat tatggagcag tagtgattag taatgggata aagtagtgaa cgaaagttat
     1261 agacacaaag agttattagc cctgttggaa gagcggaaag cactgtcaac agcagatatt
     1321 atgcagctgt taaatatttc tccggcaaca gcaagacgtg atatcagtaa attagatggt
     1381 caaggtaaat taaaaaaggt tagaaacgga gcagaagcaa ttctggctcc tacattttta
     1441 cagcacacaa aaataactaa caatgcgcag gagaaacgta gaatagcgga agctgccggt
     1501 cgtttatgta aagatgggga aagtcttatt ctgacgtgtg gttccacaat ggaaatgctc
     1561 ggtgaagtat tatgtggtag aaaattgcaa attattacca attatctacc gcttgcaaac
     1621 cagttgattg caggttgcca cgatgatgta gtgattatgg gtgggcaata taataaacat
     1681 aaaaaagtga tgctctcaat taacacccaa aatgagatga cttatgccgc taccacaatg
     1741 tttaccagtg gcaaaggctt aaccagcgaa ggattattta aaaccgatat gattattgcc
     1801 aatgccgagc aacaaatggc aaagcacgca gaacgttatg tggtgttatt ggatagcacc
     1861 aagctcggca aaaaagtcgg tatgctgttt aaagagttcg ataaaattga tttattgatt
     1921 accggcaaag aggcggatcc gactattatc caagcattaa gaaacaaagg cctggatatt
     1981 atcttggctt agcctgtagc ggtcaaattt aatcaaaatt ttacttatca tcttcaaggg
     2041 aaaactatga gaaaacataa actgggtatt tacgaaaagg ccttgccgaa aaacatttcg
     2101 tggcaagacc gcctttctat cgccaaagtc tgcggttttg actttgtgga aatgtcgatt
     2161 gacgaaacgg acgaacgctt agcccgcctt gattggagca aaaaagaacg tgccaagctg
     2221 gtgaaaacca tcatcaacac cggcgtgacc attccgtcaa tgtgcttgtc cggtcatcgc
     2281 cgtttcccgt ttggttcttg tgatgaagcg acccgccaaa aagcctatga aattatggaa
     2341 aaagccattc aactggcggt cgatctcggt attcgcacca ttcagcttgc cggctatgac
     2401 gtctattacg aagagcagga cgaaggcaca atccaacgct tccaacaagg cttagaatgg
     2461 gcggtggagc tggcggcaag caaccaagtt accattgcgg tggaaattat ggacacccaa
     2521 tttatgagtt cgatttcccg ctggaaaaaa tgggacgaga tcatcaaatc accgtggttc
     2581 accgtgtatc cagatttagg caacctctca gcgtggaacg acaacgtagc ggaagagctg
     2641 aaactcggca tcgacaaaat ctctgccatt cacttaaaag acacttacaa agtgactgaa
     2701 acctgcaaag ggcagttccg tgatgtgcca tttggtgacg gttgcgtaga tttcgccgct
     2761 tgttttaaaa cccttgccga gctgaattac cgtggcgcat tcttaattga aatgtggacg
     2821 gaaaaagccg aagagccgat tactgaaatc atcaacgccc gccgttggat cgaacaaaaa
     2881 atgcaagaag gaggatttac cctatgacgc aattaactga attaacccct gcattacaag
     2941 ccttaagaga gcgtgtgtta aaagccaatt tagaattacc gaagtatggc ttggtaactt
     3001 tcacttgggg taacgtaagt gaaatcgacc gtgagaccgg tttagtcgcc atcaaacctt
     3061 ccggcgtaga atacgatgcg atgacggtgg aagacattgt attggttgat ttacaaggta
     3121 atcgagttta cggagataaa aaaccgtctt ctgatacaga aacgcactta gaactttatc
     3181 gccaattcac tgatattggc ggtgtggtac acactcactc tcgcaatgct gtaggttggg
     3241 cacaagcagg gcgtgattta ttggcacttg gcaccactca aggcgattat ttctacggtg
     3301 cgattccttg tactcgcaaa atgaccgcag aggaaattgc cggcagttat gagctggaaa
     3361 ccggaaaggt gattgtcgaa actttccgaa ctcgaggtat tgaaccgaaa gcagttccag
     3421 gtgtgttggt acattcacac ggtccattta cctggggaaa agatggacac gatgctgtgc
     3481 ataattctgt ggtattagaa gaggtggctt acatgaattt tattagccat caaattcgcc
     3541 cacaaattgg cccaatgcaa caggaattat tagataagca ttatctgcgt aagcatggtg
     3601 caaatgctta ttatgggcaa tagctaataa cctaaaaatt attttccaaa ttatcaacag
     3661 ctttcgaggg gctttctata atcggaatag ctcctcagtc tgtttgagtt tatggataga
     3721 tccacaaata attaccattt tccttttctt gattttaatt ttgatttttg ttctaatctc
     3781 tgcctcttgt ttttacaagt ctattaagga tccgtagtag atttgaaata aacaagagta
     3841 tctttgtatt tattagaagg aaaccaaaag gtgaaaaata gaaattattt tgttgcaata
     3901 gcaacaatat tagccttatc gccagaggtt aacgcaactg aactttcaga tcagcttgca
     3961 agtagaacaa gatattatcc ttccgccacg gatagttatt atagttacta taaatatgca
     4021 ccgatttatt cgggctacta tacatcgaat agccgtaatg gttattcgtc gtggtattat
     4081 ccaggtagca atgcaggtgt aacaaccaga actgtttcga caacagttaa tggcattaca
     4141 gttacaaaag tttatacaac gcataatggg agaacaaccg ttcgtaccac ttatgctcct
     4201 gctgtgaaca aaccaattgt aaataaaccg acaccgattg ccaataaacc cgttgtagat
     4261 acgccaactc aaccggttat tgatgccaac ccaattatgg atccgccagt ccaaccaata
     4321 gttgataata aacccatcac tgatacacag cctaatccaa ttacagataa caaacctgtc
     4381 attgaatcgc caattgatga cacccgtcca gttatcatta ggcctacaac accggtaaat
     4441 cttcatcctg aacctgtagc tgttggtgta cccaatgtag gtattcgatg gaatgaccgc
     4501 tctcgtggtt acaacattga tgatcctaat aacaaacaaa atgttaagtt aactggtgaa
     4561 ggtgttaaag tcggtatctt agatagtggt tttaaaaact atttaatgca ggacgatata
     4621 ctcaaaaaat ttggtaatcg cgctgttatt attgaaacag ggcgtactgc cccggcttct
     4681 gcaacacatg gtattgaggt cgcagaaatg gttgggggag ggaactctcg caatggagta
     4741 gcgtctaagt caacgttatt acttgcggat attaccaaaa caacaaatgc gggtacagga
     4801 ttatccgcaa cagctccaat ttattatgac ttatggaata ggggagcccg tatttttaac
     4861 caatcatacg gtattccaaa gcctcttact tattttaata acaatagaaa ttctagctat
     4921 tactatttac accaatttga tggtaatgtg ttgcaattct ataaggataa agtaaaagag
     4981 gggggcttat ttgtctgggc tgcggggaat aaccgtggtg atcttgatcc atcactccaa
     5041 gctgggttac ctcattatga agctgacttg gaaaaaggtt ggatttctgt tgttgcggta
     5101 gcaaccagaa ctaatcaggg attgggtaac tttgaatgga gcaatttatt gccatattct
     5161 caagccggtg tggcaaaaaa ttggaccgtt accgcaatgg gtgactacat ctttaattta
     5221 ggaggtagaa acattattgc tagtggttca tcctttgcag ctccagccgt tacgggtaca
     5281 gcagctctcg tgaaacaaaa atatccttgg atggatggta accttatcaa acaatctatt
     5341 ctctcgacgg caaccgatat cggcgctcct ggagttgatg atgtatatgg atggggttta
     5401 cttaatattg aaaaagccac taatggccct gcgcgttttg gtaaacgact cgctttagcc
     5461 gataacgtga ctgtaaatat tccaaatgga agctatgagt tttccaataa tattaacggt
     5521 gatgcaggct tggtgaaaaa tggccaaggc gaattaattt tatcgggtgc tagtactttt
     5581 gaaggggata caacactcaa tgaaggtgct gtcactatta acggtaaaaa atatgtgtct
     5641 aaagtaaacg tatctccaag tggtacattg gttgtcaata ataccacgtt agaaaatggc
     5701 gtaaataacg agggtacagt tgtcaaccag ggttattcta cgattaagag agcttacgtt
     5761 gcttcagcac aaagtacgct agtgtcagat ttaggttcaa acttaaatgt aaaaggagaa
     5821 gttgacttaa acggctctac tttatcacta gctgcggtaa aagagggtaa agctcagtat
     5881 gtgacccaaa aaggtttatc aatgagtgct attacttctg attccgcaat taagggtaac
     5941 tttgctgcaa tagaaacctc agggttatta actgcgacgg cggaaaaaac caatgagaat
     6001 gaagtaaaag caacagttag ccggaaaaat gtcgcgactg ctttagagag ctcattttcg
     6061 gcattggata accaaattga aggaaataat ggttctacaa atgctacaaa tgatgaattc
     6121 gcacaacaag cggctacttt acaaaacagt attgcaaaca tcaatacgca aagtgtggtt
     6181 ttagatagct tatctggtca aatttatgcg tctgcgcaag cgcttacttt tgaaaactca
     6241 gaaacagtga ataaagatct ctctaaccgt cttgtaatgt taggaacgct ggataatgta
     6301 ggtgagactg ctggtgtgtg agtaacgggt atttatggga atggtactct aaaagaaata
     6361 gggtttggtg aaggtaaaac caatacctat gctggccaaa taggttttga taagccagtt
     6421 acagaccatg tgattttagg tgcggcactt aattattcta aagcaaaagt gaacttcgat
     6481 cgttatggcg gtagttcaaa agcagatgga ataggtgcat cattatatgc ccgtataggc
     6541 aataagcata aaacaccttg gtatttacaa ggtcgagccg gttatggcag tgttgattca
     6601 gatgtagagc gtcatattat tttggcggat aataacgcaa gtacagcaaa aatcaaccac
     6661 cgagacagag tattttctgc ttatgttgaa tcaggctatg acttcaagca aggtcgcttt
     6721 gcgttaactc catttgtcgg ttttagtcat gatgttgtca gacgtggcgc attcagtgag
     6781 caaaacagtc aatttggtct caccgcggac aaagcaacct ataaacaaac aagcggattg
     6841 attggtttac gaacatcttt agaggtgaat ttagctggga tgaaaacgac tttccaaggc
     6901 tatgtaaatc atcaaaaagc gtttaatcga gaagatctta gctttaaagc aagttataca
     6961 ggtttacaag atgcaaaatt cgacgtaaaa ggtattgggc ttgcaaaaca taaaacgtgg
     7021 gtagggattg gtgcattggc ggaagtaaat aaaaatactt ccttatatgt gaattacgac
     7081 cttaaactcg caaaaaatag tggtcacaac aacgttgtga ctgctgggat tagaattaat
     7141 ttctaactga atctttttaa acaaaacggg cgtttatacg cccgtttttt tatagttata
     7201 gagataataa tgttatttta tacttcattt aattaggcaa tctttacagc ttggtaacta
     7261 cactgtggat taacaatccg atcacgagaa gcaataattt gtaactcata aagccctgat
     7321 ctagcggttt gttccattac ctcattgacc gcatttgcag tgtgttcaaa ggcttctaga
     7381 tcggattttc catttaataa gttcgccaca aatagccctg cggttaaatc gcctacaccg
     7441 acaggttctt tggcaaattg gtagagtggg cggctgatgt gccaaatgcc atcttggttg
     7501 gcgaacagca tttcaaattt gttgctctct ttgccgactc ggcttaaatg tttaaccaat
     7561 acttttttcg ggcctttggt taaaatcact ttcactgcct cgaccgcttg ctcaaaattt
     7621 tccaccgtta aaccggttaa ttctcttaat tccactaaat ttggtgtgat aatgtcggct
     7681 tgtgccattg ccagattgat caatccctct tttacgccgt ctgccacaat gcagcctttg
     7741 tcggggtgtc ccattaccgg gtcgcataca taaattgcgt ttggattttc cgctttcact
     7801 ttatgaaaag cctgaataat ttcggctact tgttctgccg agccgatata gccagagagt
     7861 acggcatcgc aacgatgcag ttcaccgata ttgtgaatgc catcaacaat gctaccgatt
     7921 tgttcttttg gcatcaccat accgccccat tttccatatt gggtatggtt ggaaaactgt
     7981 acggtattta acgcccatac atcaacacct aacagctgca taggaaaagt tgaagcctta
     8041 ttgccggcat agccgtagac aacgtgcgat tgtatggata gtatgttttt cattcattca
     8101 ctcctttaaa atttcaatat gtgttgtttt ttagtcattt gaagttgcaa ggacttgaag
     8161 ctcatcaaga aatctctata atcccgcctc gttctcttat ccattcaata tgccgactta
     8221 gctcagtagg tagagcaact gacttgtaat cagtaggtca ccagttcgat tccggtagtc
     8281 ggcaccatca cctttatttt ctgatgagtc ccctttaatg taaaatggca agattttgca
     8341 aattttcaca aaaatcttac cgcttgtagg gtgaaattga attagacgta accgtaattc
     8401 attaagcgtt gataacggcg ttccagtaaa tcctctttat taagttgatc taaccctgcc
     8461 aaatcttcaa caatacgctg tttaaggttt tgggcgattt gttcaacatt gcgatgagca
     8521 ccgcctaagg gttccggcac gatgttatca atcagctcaa gctcttttaa acgaggggca
     8581 gttaaaccca ttacttctgc tgccgttgag gctttatcgg cacttttcca cagaatcgag
     8641 gcacagcctt caggtgaaat taccgagtaa gttgaatatt gcaacatatt gaccttatcg
     8701 cccacaccga tcgctaatgc accgcccgag ccaccttcgc caattaccgt acagattact
     8761 ggcacggaaa gttgagacat ttcacgcagg ttacgggcaa tcgcttctga ctgaccacgc
     8821 tcttccgcac caatgcccgg ataagctccc ggtgtgtcga taaaggtgat gattggcata
     8881 ttaaagcgtt ctgccatttc cattaaacgt aacgctttgc gatagccttc aggagcaggc
     8941 ataccaaagt tacgtttcac tttctctttt acggtacgac ctttctgatg accgataacc
     9001 atcaccggac gtccgtccag acgggcaata ccaccgacaa tcgctttatc atcggcaaag
     9061 gcacggtcgc ctgccagttc gtcaaattcg gtgaaaatat gctcaatata atctaaagtg
     9121 tacgggcgat tggggtggcg tgccatacgg gaaatttgcc acgcatctaa attagcaaag
     9181 gttttcttgg ttaattcctc gcttttcttt tgtaaacgct tgatttcatc atcaagatta
     9241 attttgtcat cttgctcgct gactgctctt aaagactcga tcttggcttc caattcagca
     9301 atcggcaatt caaaatctaa aaattcttgg ctcattctat tttccttttt atgggcaaaa
     9361 ttatggggca ttatcgctta tcagtcggtt attgtccatt ttcaattacg ctaaattgac
     9421 ccatcgtacc tctatcacgc agcataaaat cgctcacgcc aaaggtaaat ggcagttgct
     9481 caggggcagt attatcaaag cgaaccaaca gcgtgatttc ttgctccgcc tcgagccaaa
     9541 cggtgtcccg ccacgcaagt tgtttgatag ggtattgctt gcggtttagg gtttcaataa
     9601 tgaattttgc accttgtagc gtaaatccga ccgcttgtgt gctatgtaaa taccaacgct
     9661 caacgctgcc tttttgcgcg gtaaaatcaa tgcgttttgg gtcaaaccgc ttttggttga
     9721 ttaaataatc agccggacga atggcaaaac gtctctcttg gctgattttc aggttaaatt
     9781 cctctaagtt gaaagccggt aaactcggtt tattattcaa taccgctgcc attccttcag
     9841 ggcgaagctc taaaatcacg ttatcattca attcgtcgtt gtcagcaaag acctgcccga
     9901 ttttgtaaaa aatatctctt ttttgaccgc ttatcagcga taccgctttc ccttcgctta
     9961 aatcgaccaa cacttccacc ctttcactcg gtgcaagttg aaccgattcg gtttcaagtg
    10021 gctctgccag cattcctaag ccggtggcga ttaggtacaa cggctgctcg ttatctaagc
    10081 gtaattcata agcacgggaa agtgaggcat tgacgatccg caatctgacc cagccacgag
    10141 gcacgttaaa ataaggcgat tcctgcccgt tgacaaataa tcgcttaccg aaaaattgtg
    10201 aggtgttttt atctaacacc tgtacgccgt ctttgttgag cagctggtct tgcagaatta
    10261 acggaatgtc gttcacgcca tatttattcg gtaaagtggc ttttttgctc tgttcatctt
    10321 caatcatcca caagccggct aagccacgat acacttggaa tgccgagttg agcattgtat
    10381 cggcgtgata ccaacaagta caagccggtt gattgataga aacaatcggc gaccagctac
    10441 ttttggggtc aagggaacga tgcaccgtgc caatcatctc acttgaggct tgcaatcctt
    10501 gaatattcat tgagattttt tgcggtaaat tattgagata agttaatttg acaaaatccc
    10561 ccgattttac ccgtacggtt ggggcaagat attgcccgtt cacaccccat acatcaacca
    10621 atttgccttt atcaaattgg gtttgtgcgg ggcgtaaatc caaccgaacc ggctttcctc
    10681 gcccaatatc cattaaaggc ggaatgttca gttgcggtcg ctctgctgct aatagcggtg
    10741 ttggcactgt tgcaaatcca gccgcaaggg cagagccttg taaaaattgg cgtcgggtga
    10801 tctgtttttt catattaggc gttttttgca gtgttttcta actgttcgac ttcttgatta
    10861 agttgttcaa attttgccaa cataatttga tggcactttt ccatcagttc tttgacgttt
    10921 tcacgggaat agcctgaagt atcaatcgga tcaagcattt cacagattac tgtgccgtta
    10981 tttgaacggt tcaaatccac tttattgtgt aaattagagc aaactaccgg cacaatcggc
    11041 acgccggcag aaatggctaa atggaatgca ccggttttaa atggcagtaa accacgccca
    11101 cggctacgag taccttccgg gaacatccac accgacacat tgcgttcttt cacgatttgc
    11161 cctgctttat tcattgtttc aatcgcactg gaacgctttt cacggtgaat aaaaatattg
    11221 ccggttgccc agtaaaccaa accgaaaaac ggaatccaaa tcaggctttt tttgccgata
    11281 ctcaccgtct tttctgccac cattgaggaa atcgttacca tatcgtaatt gttttgatga
    11341 ttgccgatat agacaaccgg atagttaatt tccggctttt tgcgggtaat taattccaac
    11401 ccgactaatt tatgcaaagc accaaaccaa cgagctacca cacccacgct acttgggtga
    11461 cgtaaacgca gtaacgcata aatagtgcca aaaatagaaa tgaaaatagc ggcaagggca
    11521 acaattaaaa tacgaaaaat ttttaacata atggaataac cttaaaaata aattgtctag
    11581 gagtatagca aaataaggtt attttctaaa ggctatccac attaaattga tagtttccta
    11641 ttttgcaaaa aatcggtata tttaaaccgc ttgtttattt gataaggaga aaaaaatgag
    11701 taaagtgact ttagcaggaa gcccgattga tgttgcaggc acatttccac aaaaaggcga
    11761 tgtggttgcc gattttacct tagtcaacag tgatttaagt gatgtttcgc tttccgattt
    11821 tgatggcaaa cgcaaagtgt taaatatttt cccaagtatt gacaccggca tttgtgccac
    11881 ttctgtgcgt gtgtttaatc aaaaagcggc tgatgtagcg aatacggttg tcctttgtat
    11941 ttcagcggat ttaccttttg cccaagcacg tttctgtgga gcagaaggaa ttgaaaatgc
    12001 caaagtatta tccagtttcc gtcatcgtga tttacacaac aaactggggg tagatattat
    12061 ttcaggtccg ttggcaggct taacttcacg ttcagtaatt gtgttagatg aaaacaacaa
    12121 ggtattacac agcgaattag tgccggaaat taaacaagag ccggattatg aggcggcgtt
    12181 agcggtatta taatttgcag aattatgctg gaaaataacc gcttgtaaag caacaaaaag
    12241 cccgttgatt caattcaacg ggctttgttt ttagtgatta tttcttacga gagtgtaagc
    12301 cttttttctc aaggttacgg taaatcaacc attgctgtgc aatcgtgatt aagtttgagg
    12361 ttaaccagta aagcactaag cctgatggga accataagaa gaacacggta aagataaccg
    12421 gcataaaggt cattactttt tgttgtaccg ggtcagctac cggtgttggc gacatttttt
    12481 gtaacaagaa catcgaacca cccattaata aaggcaaaat gtagtatggg tcttgtgccg
    12541 ataagtcttg aatccagccg aagaaaggtg cgtggcgtaa ttctaccgct tccataaatg
    12601 tccaatataa agcaatgaaa atcggcattt ggattaaaat tggtaagcag ccacccattg
    12661 ggttcacttt ttcttctctg tagagtttca tcatctcttg gctcatacgt tggcggtcgt
    12721 cgccgaaacg ttcacgcatt tcagtaatgc gaggttgtaa catacgcatt tttgccatcg
    12781 aggtgtattg tgctttggtt aatgggtaaa gaatggtttt cactacaata gtgacaccga
    12841 taattgccaa gccccagttg cttacgattt tttgaattgc ggttagtaac cagaaaagcg
    12901 gtttagcgat aaaccacgcc cagccgtaat ctacggttaa gtctaggtga tttgctgatt
    12961 cgcccatttc tttctgatct ttagaacctg tccaaagttt gctatttaaa ttagcctctg
    13021 aatttggcgc aatcgtagtt aaagggccac ggtaaccgat agttgctacc ccattattgg
    13081 tgcgtgaata aaggctgtta tccgcatctt ggtttggcac ccaagcagac acgaaatagt
    13141 gctgtaatac cgcaatccaa cccgctttgg tgtccacgct taagttggct ttttccatat
    13201 cttggaaact gtattttttg tagttggttt ctgaagaaga ataagccccg ccggtgtaag
    13261 tcggcatggt taaattgccg gaactttcaa ctatagagtg ttttaattgc ccgtaaggct
    13321 gaacttcaat cgcttgtgca gtattatttt tgatattgaa gtttacccca acatcgtagc
    13381 taccacgttt tagcgtgaaa gttttggtgt aaaccacacc ctctttttca aaggttaatg
    13441 gtacagaaat ttcattttgc ccttcggcta atttgaagct atctgccgtt accgcatatt
    13501 gtgggcgacc ggcattggta tcaatcccgt ttttacccac taaaccgctt tgagcaacgt
    13561 agattttgtc gccgtcttgc tgtaatagtt taaatggcgt gttagagtgt aattcagcat
    13621 tgtgttttaa caaatctgag ccgatgacat caccgcctaa ggtatcaata gtcagacgta
    13681 atacatcgct ttccacggta atggttttac cttgcgtgat gctatctgcg attggattat
    13741 tggcattgga agcagacgga agatcacccg cttgttgggt ttgtgctgcc acttgagctt
    13801 gtttttgtgc ttgaatttca ggatcaaaat cttgttgcca ttgtgtaaaa atcaaaatag
    13861 acacaagaaa taaacccatt agcagtaagc cacgattatt catgttggaa ttctcttgtt
    13921 agtaatggat aaaaagaaag gtattttata ataggttagc ttgttttcca aacgcctttt
    13981 atgaacaagc ggtcggattt tccaagtttt ttgcaaaatt ttcggagaat ttgaccgctt
    14041 gtggagaata agaaactagc gtttatctaa ggttttggtg gaaaggcgag caactgcaca
    14101 caggttacct tgttcgtctt taatgtcgat attccaaacc tgtacttcac gccctaattt
    14161 cagcggtgta gctttggcaa tcgcttttgt gcctgccgga atcgatttta aatggctgat
    14221 gtttagctcc attcccaccg caatttgatg gggttcacac accattaacg cccctgcatt
    14281 ggcagtagtt tccgcgagtg ctgccgatac accgccgtgc aatacgccaa acggctgttg
    14341 agtgcgttcg tccacctgaa gctgtgcttc aatccaatct tcaccgatag cggtaaattc
    14401 aatccctaag tgtgacaccg ccgatttttg gctgagtgtg tttaattgtt cacaagttgc
    14461 tgtttgcttc caaatggtca ttattatttc ctgtctaata aaaaagctgt ctatttagac
    14521 agctttcatt atctctttga gcgaaacata ctacgccaat agtacattgt ttggtcgccc
    14581 atattacctt aagccatata gcactaagga ggtgatttat gtgagaaaat cagaagtttt
    14641 ctttctggtt gcgttgctaa tattattagc aacttccgct agcattaaag tactcaccat
    14701 acttgatgtg ctagtttggt tattcgctag ataacctaaa atcagacaga ggcaggctgg
    14761 ttacctgcct cgctttttat tctaagtact tttaggttac atttcaacct taaatcacgt
    14821 ccagcaacgc ttgaattggg tgtttaatgc cttgttgttc aaaacgtttg acttgcgagc
    14881 ggcaggagta gccggttgct aggcaacgct caagcggttt attttttaat ttaatttgcc
    14941 atgattgctg ataaatcgct ttcgacattt caacgtgttt ggtttcgtgt ccgaacgtgc
    15001 ctgccattcc acagcaaccg acattttcat tgagtaaggt ttcaccaaaa tgggtgaaaa
    15061 tagcttgcca ctctttcatt gagcttggca aggcggtgga ttcggtgcag tgagcaaata
    15121 aataccacgg caagcggtcg gaatttgccg attttttgca attttccagc tcgccgtttt
    15181 taatcacttc ccttaaccat tcgtgtgcca ataagacttt gaaatccccc cgagcttctt
    15241 tgaggatctc tttgtattct tcacgataaa gcaaagttaa ggcaggatcg acagccacca
    15301 tcggtagccc caatttcgcc acacggttta aaaaatcggc ttggtttttc gctgttttcg
    15361 caaatttatg caaaaagcct ttaacgtgtt gtgctttacc gcttggcttg aatggcaaca
    15421 caatcggctt aaagcctaat tttttaatga gagcgataaa gtctgccacc actttggcat
    15481 cgtaataaga ggtaaacgga tcttgcacaa taaacaaggt tttgcaattt tttgcagaaa
    15541 aatcaccgct tgtagcttgt tgttcaaggc tttctaagct ctcgccttgg tagccgactt
    15601 ccaccaactg atgttgcaga tttggcactg acaatgccgg caaataggtc attccaatgg
    15661 ttttgtttgc agctttttcg gcaaaagttg aggtgctgaa aaagttaaaa aaacgtggtt
    15721 gtttcgccat taaaggggcg ataaattcta agtttgagac cacataatct ttcagtggtc
    15781 gcaaatagcg gctgtggtaa agctcgttaa attgcgatct gaacgtcggt acgtcaattt
    15841 tgattgggca ttggctggca caggctttgc aggcaaggca ggtgtccatc gccgctttga
    15901 cttcgtgtga aaaatcatac tctttgtttt tattgagcgt gttgcgaatt tttgcaacaa
    15961 aatcagctaa tttgaccgct tgtttgcgag gtttgaagat caaatcgtcc ggcgaaacgt
    16021 tttgttcggt aagtaatctg agccattcac gcacaagagt agcccgccct ttcggtgagt
    16081 aaagacggtt gcccgagact ttcattgaag ggcacatcgt gctgtgtaca tcaaagttaa
    16141 aacatagccc gttgccgtta caattcattg ctcctttaaa ttcttctcgc acttggattg
    16201 gaatttggcg gtcaaaatcg gctcgcattt gtgagtcaat cgggtagagc gaggtttcgc
    16261 tttctaatgg tgtacaaatt ttacctgggt tcaagcgatt gtacgggtca aataaggttt
    16321 tgatgtaacg gagttcttgc cacaaagttt cgccaaagaa acgttcgccg tagtaagaac
    16381 gcataccttt gccgtgttcg ccccaaatca agccaccgta tttggcggtg agttcgacta
    16441 cttggtcgga aatttgctta aaggtttcca cttgggcttt gtcgcataag tcaagagcag
    16501 ggcgaacgtg tagcacgccg gcatcaacgt ggccgaacat tccgtaaggc agattatagc
    16561 gatctaataa agctcgaaat tccacgatgt aatcggcaag gttttccggt ggcacagcgg
    16621 tgtcttctac aaaggcgatc ggttttgccc agcctttggc attgcctagc aggcctacgg
    16681 cttttttccg cattgcgtag attttctcga tagaatttac atcactcgtg agttggtagc
    16741 cgataatccc ggcttcatta tttgccattt tttgatcaag gctttggcag agtgctttca
    16801 cttgggtttc aatttgagtc tgatcgcttg aggcatattc aacaatgtta atccctaaaa
    16861 tcgggttggc tgggtcttcg gttaataaat ctgacaccga atgccagacg atatcttgct
    16921 tggcaagatt gagcactttg gaatcaaccg tttccaccga taaggctttt gcctctaaca
    16981 taaaaggagc ggagcgtaag gcggcatcaa aggaattata tttaatatta attaaggtgc
    17041 gatatttggg aatcggcagt aaattcagtt tggcttcgca aataaaggca agcgaacctt
    17101 cagaaccggt taaaatacgg gtaaggttaa attcgctttc accatggtta aacacatttt
    17161 ttaaatcata gccggtaata aaacggttaa gctgaggcag ttcgttcagc actttggggc
    17221 gtaatgtttt gcaacggttg aaaatttcac ggtgcaaggt tttgccgaga gcggagagat
    17281 ttaacccttc aatatgtgca aaaaaatcgg cagatttgac cgcttgtgtc tctaaaatat
    17341 cgccgttaat aagcacagat ttgatggcaa gaatatgatc agatgttttg ccatattgca
    17401 aagaaccttg cccagaggca tcggtgttga tcattccgcc taaagtcgct cggttactgg
    17461 tagaaagttc gggggaaaag aaaagtccat gtggttttaa aaattgattg agctggtctt
    17521 tcaccacgcc agcctgcact cgaacccaac gctcttccac attcagttcc aaaatctgat
    17581 tcatatggcg ggagagatca acaataatat tattgttaag agattgcccg ttagtgcctg
    17641 tgccaccgcc tcgtggagta aaagttaaat gttgatattc tggcttttgg gcaagtttgg
    17701 ttagaattac gacatcagaa acggatttcg gaaacagaat agcttgcgga atttgctgat
    17761 acacgctgtt gtctgttgcc agtgaaagcc tatctgcata atttgatgca atttccccac
    17821 taaaatgctg attgacgagt tcagttaaat aggattgggt ggctttatcc agttgcggta
    17881 cttcagagag tctaggtatc ataagctgtg tttggttttt tataataaaa gcactaaatt
    17941 ttgcttatct tagcaatttt tctaaaaatt gggaatggaa
//
LOCUS       DANLXQ010000035        17690 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.035, whole genome shotgun sequence.
ACCESSION   DANLXQ010000035 DANLXQ010000000
VERSION     DANLXQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17690
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.035"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXD94_000912"
     CDS             complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXD94_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL6285796.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXD94_000913"
     CDS             complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXD94_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL6285797.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXD94_000914"
     CDS             complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXD94_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL6285798.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            3255..3845
                     /gene="rsmD"
                     /locus_tag="PXD94_000915"
     CDS             3255..3845
                     /gene="rsmD"
                     /locus_tag="PXD94_000915"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL6285799.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LYVETEKNHPPLELPQGWQILKEKAAGMVVSRLIQL"
     gene            3929..4243
                     /locus_tag="PXD94_000916"
     CDS             3929..4243
                     /locus_tag="PXD94_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL6285800.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     EK"
     gene            4256..4768
                     /gene="hpaC"
                     /locus_tag="PXD94_000917"
     CDS             4256..4768
                     /gene="hpaC"
                     /locus_tag="PXD94_000917"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL6285801.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVDTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            4886..5758
                     /gene="tehB"
                     /locus_tag="PXD94_000918"
     CDS             4886..5758
                     /gene="tehB"
                     /locus_tag="PXD94_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL6285802.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            5896..6933
                     /gene="purM"
                     /locus_tag="PXD94_000919"
     CDS             5896..6933
                     /gene="purM"
                     /locus_tag="PXD94_000919"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL6285803.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            7011..7289
                     /locus_tag="PXD94_000920"
     CDS             7011..7289
                     /locus_tag="PXD94_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL6285804.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
     gene            7432..8859
                     /gene="aspA"
                     /locus_tag="PXD94_000921"
     CDS             7432..8859
                     /gene="aspA"
                     /locus_tag="PXD94_000921"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL6285805.1"
                     /translation="MSDVRIEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVP
                     EFVRGMVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAG
                     TSVNMNTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSIT
                     KLINKITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLK
                     RTAELLLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAY
                     VMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEV
                     VNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDG
                     ITANRDICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEE
                     QLDDILSIENLMNPTYKAKRFTEEE"
     gene            complement(8934..10952)
                     /gene="rep"
                     /locus_tag="PXD94_000922"
     CDS             complement(8934..10952)
                     /gene="rep"
                     /locus_tag="PXD94_000922"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL6285806.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVDHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
     gene            11200..12315
                     /gene="potA"
                     /locus_tag="PXD94_000923"
     CDS             11200..12315
                     /gene="potA"
                     /locus_tag="PXD94_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL6285807.1"
                     /translation="MEKVEQKAIIELKNVTKSYGSKTILKNLNLTINDGEFLTILGPS
                     GCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVFQSYALFPHMTIFENV
                     AFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQQRIAIARAVVNKPKV
                     LLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEALTMSDRIIVMNAGKI
                     AQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKANVEGRICNIKVEDLE
                     VQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMTLESSVRLDHNGMNVL
                     VSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
     gene            12302..13165
                     /gene="potB"
                     /locus_tag="PXD94_000924"
     CDS             12302..13165
                     /gene="potB"
                     /locus_tag="PXD94_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL6285808.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            13165..13932
                     /gene="potC"
                     /locus_tag="PXD94_000925"
     CDS             13165..13932
                     /gene="potC"
                     /locus_tag="PXD94_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL6285809.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFSLTLVVLSQLVVKKKV"
     gene            14133..15056
                     /gene="oppB"
                     /locus_tag="PXD94_000926"
     CDS             14133..15056
                     /gene="oppB"
                     /locus_tag="PXD94_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL6285810.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            15071..15970
                     /gene="oppC"
                     /locus_tag="PXD94_000927"
     CDS             15071..15970
                     /gene="oppC"
                     /locus_tag="PXD94_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL6285811.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            15980..17575
                     /locus_tag="PXD94_000928"
     CDS             15980..17575
                     /locus_tag="PXD94_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6285812.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
BASE COUNT         5119 a         3660 c         3526 g         5385 t
ORIGIN      
        1 agcaataata tattgcttat agtaaaaaac caagttccta ggaactggtt tttatctgca
       61 attatttctc taaaaacaac cgcttgtatt agcctctact accaatacgg aaaatatttc
      121 gcattgtcgc aatttttgcc gacagccagc caataaacac acaaagcacc actaagaaga
      181 aggcttcgct gaaatctaat ccgttcagtt caaattgtac ggtaaacata tcagtcacat
      241 acttcactac accggtgaaa tagctaatcg ttaccgcact aaacagcacc gctaacaggc
      301 tgccaaacaa gccgtaaatc atacctgtgt ataaaaacgg acgggcaata aagtgatcag
      361 ttgcccctaa aatttgttgg acttcaatcg ctgctttgct attggcaaca tcggttcgca
      421 cactgtttcc aatcactaaa aaaactgcca acagcattaa taaggtacac accaccgcaa
      481 tacgagcaat cagccaagtt agagcagtta atttttccag ccagccgtta tctaaacgaa
      541 cctcctgcac ccctttaatt tgttgcaaac cgttgcgtag ctccaccatc gtatcagcat
      601 tattaaaatt tgctttcggt tttaaggtaa cgaccgcagg caacgggttt tcatctaaaa
      661 tatctaacgc ttcaccaaag cctgaccaag aacgaaattc gtctaaactt tgttggcgag
      721 aaatatagtt aaatgactca attttatccg tctcaaaagc acgaatgcga tcaacaacgg
      781 tattcacatc gtgttctgcc aaatttttgt gcagataaac ggttaattgt ggctcagggt
      841 aaaattgtgt tgctgcctcg tgggtattct tccaaagtaa ataactcacg gttggaatcg
      901 taagtgagac cgcaatcacc agcaccgtca gaaaagtgcc gagcttgcgt tgcattaaat
      961 cttgccaaac cgctcttaaa gagtagttgg tttgagcacc taatttacta aatgaacgaa
     1021 tcataatatt tccttaaaat ttaagatgcc cttgctccag cactaaacac ggctttggac
     1081 gctttgaaat aatattggta tcgtgggtgg caatcagcac cgttgtgccg gcttgattaa
     1141 attcctcaaa caaacggaaa atttcaaacg agagcttttc atctaagttt ccggttggct
     1201 catcggctaa taacagcgtt gggcgatgaa caatggccct ggcaatatcg acacgttgtt
     1261 gctcaccgcc ggaaaggtgc aacggcagat agttcgcttt atctgccaag ccaacacgct
     1321 caagggcaat gcgggcttca cgctccacaa tcgcttggtt cattcccaaa ataatcaatg
     1381 gtaatgcgac attatccaaa atggttcggt cggttaataa gcgataatct tgatgcacca
     1441 tcccgatttg gcggcgtaaa aacggcaatt catgatgagc aagtcgggta atatcgtgtc
     1501 cgttaaacaa aatttgcccg ccattggcac gctcaatccc cataattaat tttaatagcg
     1561 tgcttttccc cgcaccggaa tgcccggtaa tatatgccat tccaccggca ggtaggtgga
     1621 aatcaatgcc ctgtaaggct ggctttccgc ccttataaac cttgttcaca ttagtgaatt
     1681 taatcattct gttcgtcctc gtggctaaat aaggcttcaa taaattcttc tgcattaaac
     1741 gggcgtagat cttcaatttt ctcgcccacc ccaataaagc ggatcggaat attgaattga
     1801 tcggcaatcg caaaaatcac gccacctttt gccgtaccgt ctaatttagt taaggtgata
     1861 ccggttaaac ccactgcctc attaaacaat ttcgcttggc tgatcgcatt ttgcccagtg
     1921 ccggcatcaa gcgtaagcat aatttcgtgc ggtgcggttt catcatattt tttcatcacc
     1981 cgcacaattt tcttcaactc gtccatcagg ttatttttgt tttgcaaacg ccctgcagta
     2041 tcagcgatta gcacgtcaat cccttttgcc gcagcggatt gcatcgcatc aaaaatcacc
     2101 gaggcagaat ccgaaccggt gctttgtgcc accaccggaa tgttatttcg ctctccccaa
     2161 acctgtagct gttcaactgc cgccgctcgg aacgtgtctc ctgctgctaa cattaccgat
     2221 ttgccttctt gttggaattt acgggcaagt ttgccaatgg tagtggtttt ccccacgcca
     2281 ttcacaccaa ccattaaaat gacatacggt tttttcgctt ccaacactaa cggttgagcc
     2341 accggtttta gcacttcacc cagttccact ttcaactgtt gatacaacaa atctgcatct
     2401 tttaattgtt gcttggtagc atgttgcgtt aaattgttga tgattttttg tgtggtcggc
     2461 atacctaaat ccgccacaag caactgctct tccagctctt caaataactc atcatcaatt
     2521 ttcttgccac ggaagaaatt acggaagccc gaaccaatat tctgcttggt tttaatcaag
     2581 ccttgaatta aacgactgaa gaaaccgcct tgactcggtt tttcttgata ttcatcttgg
     2641 atagtagatt cttcaactgt gtcttgaaca ttatttacta attcagtttc agcaacttct
     2701 tcagccacag cctcttcgac ttgataagta acttgctctt gttcgttgag atctaactgc
     2761 tcagttgcaa tattttgaag attttcgacc gcttgttcgc ctttttgttc ttcaaatgtt
     2821 tgttcgattt tatcttcaac atcatcaaag aacgctttcg cctcttcaac tttttcttca
     2881 acaaactctt tgaaatcttc gactttttgt tcaatggtat cttctacttg ctcagcaaat
     2941 tgctcaattt tttcttcttt ttcctcaaaa tagtcttcta actgatctac cttttcttca
     3001 attttatctt ccagctcatc taatttttca gaaaacgact tctcagtttc cggttcaaca
     3061 ataacaggct gttccggttg aatactctct tgttcttgtt gaatctcttt ctgttcagct
     3121 tcttctttct taccaaatcc aagccaagac caaaaccctt ttttcttctc tgacatattt
     3181 ttctctcatt tctcgtaaaa atagactaaa cttacatagt ctatcaaaaa atcccttatt
     3241 ttcctttagt gagtatgaaa aaaaatcgaa attcccccca atctgttcag caaatgggcg
     3301 aagtacgggt tatcgccgga ctgtggcgag gcagaaaatt acctgtacta aatgccgagg
     3361 ggcttcgccc gacaaccgat agagtaaaag aaacgctgtt taactggtta atgcacgaca
     3421 ttgctcactc acgctgtctt gattgctttg caggcagtgg ctctctgggc attgaagccc
     3481 tttcccgcca agcacaagcg gtcgtttttt tggaaaaatt tgcaaatgct gccaaccagt
     3541 taaagaaaaa tctccaagca ttaaaatccg aacaaggtag cgttattcac actgacaccc
     3601 tcgcctattt agcacaaaaa aataacggtg agccatttaa catcgtattt atcgatccgc
     3661 cttttcatca agggtttgta cctcaagtgc taaacttgct ggcagaaaat aactggctcg
     3721 ccccgaatgc gattttgtat gtggaaaccg agaaaaatca cccgccatta gagctaccgc
     3781 aaggctggca aattttaaag gaaaaagcag ccggaatggt ggtaagccgt ctgattcagc
     3841 tataatcttt ttgtttttta taactaaaaa atataaaata aaacttatct atctaacatt
     3901 ttattaacac tcaaattaga ataacgaaat gaaaaaatta acccctgatg aagccattga
     3961 tattgcctac gatattttct tagaaatggc aggcgaaaat ttagatcctg ccgatatttt
     4021 actgttcaac ctccaatttg aagagcgtgg tgcagtggaa atggtcgaaa cttctgaaga
     4081 ctgggatcaa gaaatcggga ctttaattga ccctgatgcc tttgccgaag tttgggttgg
     4141 cttagtcaat gaacaagatg aaatggacga tgtattcgcc cgtttcttaa tttcccacga
     4201 tgaagaaaac cgtgaatatc acgttgtttg gaaagaaaaa taaaaaggag aaaaaatggc
     4261 taatatcgac actaatcaac caatagaagc ctcgcttaaa tttcgtgaag caatggctca
     4321 cttatcttct gcggtgagca ttgtgacaac caatggcgta gccggcaaag cagggctgac
     4381 ggtttcgtct gtttgttccg ttaccgattc cccaccaacg ctcttatttt gcattaataa
     4441 aaacagccac gttcacgata ttatccaaca aaacggcaaa gtatgcgtta atgtgttaag
     4501 ccacgaacaa gaagacttag caaaacattt tgccgccatt ttagaaagta caatggaaga
     4561 acgctttggt tgggatattt ggaacaacgg ctttgacgat cagcctgtgt tacgcagtgc
     4621 tatttctgct ttacaaggca atattgttga tacgcacgca gtgggtacgc atactatttt
     4681 tgtggtagaa gtgagccata ttgacgtaga gccgaaccac agtttagttt attttgcccg
     4741 ccaatttaga agcgtgaaca ttgactaaaa tatatttaac agaaatttga tcaaatccga
     4801 cttttttagt cggatttttt atttcctgtt tttaaaacag tgctaaaatt ttgccgttta
     4861 tgtttgactg acaaaaggaa acaaaatgga aaatttaatt tgctacaaac aaatgccggt
     4921 gtggacaaaa gcaaccttac cacaaatgtt ccaagaaaag cacaacacca aagtcggaac
     4981 ttgggcaaaa ctcaccattc tcaaaggttc tctcaaatat tatgaattaa ccgaagaggg
     5041 agaaattatc aaggaagata tttttgatgc aaaccaccaa cgcccaatgg ttgagcctca
     5101 actttggcat aaggttgaag ctctttctga cgatttagaa tgccagttgg ctttctattg
     5161 caagccggaa gattattttg ctaaaaaata taatctgacc gctacccatt cagaagtgct
     5221 aaatgccgtc aattatacca aaggtggcaa agcgttagat ttaggttgcg ggcgaggacg
     5281 caatgcactt tatctgaatt tactgggttt tgatgtcact gctgttgatc ataatcaaga
     5341 aagcattgat tttctcaact atatgatcga aaaagaacaa gctcaaaaca ttactaccgc
     5401 tgtttatgat attaacgagg caactatcgg ctcgcaaaac ggtgaatatg acctgattat
     5461 ttccactgtt gtaatgatgt tcttaaaccg tgaacgcatt cctgccatta ttgagaatat
     5521 gcagaaaaac actaaagtcg gcggttataa cttaatcgta tgtgcaatga gtaccgatga
     5581 ctacccttgc ccgatgccgt tctcctttac cttcaaagaa ggcgagctcg ccgattatta
     5641 tcaaggttgg gaattagtca aatataacga agatttagga catttacaca aaactgatga
     5701 aaacggcaac cgcattcaac tacgttttgc gacaatgttg gcgaaaaaaa tcagctaaac
     5761 ttaaccgctt gcagggggaa ttagcttccc cctaattcat caaatccatt ctatttattt
     5821 tcactttttc attccgcaaa cgtttgcttg tgctataatg gcttcgtttt ttagtcattt
     5881 actgttgagg ttaaagtgag caacacacaa ctaagttaca aagatgccgg cgttgatatt
     5941 cacgcaggaa acgagttagt cgaacgaatc aagcctgatg ttaaacgcac ccgtcgccct
     6001 gaagtaatgg gcggattagg tggttttggt gctttatgtg ctttaccgac aaaatataaa
     6061 gagccgattt tagtttccgg taccgatggc gtcggcacaa aattacgctt agccattgac
     6121 ttaaacaaac acgatactat cgggcaagat ttggtagcga tgtgtgtgaa tgaccttgtc
     6181 gtgcaaggtg ctgaaccgct tttcttctta gattattacg ccaccggtaa attagaggtt
     6241 gatgtagcca ccaatgtcgt caaaggcatt gcagacggtt gcgtgatttc aggttgtgct
     6301 ttagtgggag gtgaaacggc tgaaatgccg ggaatgtacc acgaaggcga ttacgattta
     6361 gccggattct gtgtcggtgt ggtagaaaaa tctaaaatca ttgacggttc aacggtgaaa
     6421 accggtgatg ccttaattgc attagcgtcc agtggcccgc attccaacgg ctattcgtta
     6481 attcgtaaag tattggaagt cagccgatcc aatcctgcaa ccgatttatt ggaagacaaa
     6541 cccttaagcg aacacttatt agccccaact aaaatttatg taaaatcaat cctaaaatta
     6601 gttgaagaag ttgatgttca cgctatcgct cacttaaccg gtggcggttt ctgggaaaat
     6661 attccacgcg tattgccgga atctgccaaa gcggtgattg atgaatcgag ctggcaatgg
     6721 ccggcgatat ttagctggtt acaggaaaaa ggcaatatca gccgttatga aatgtaccgt
     6781 actttcaact gcggtgtcgg aatggtgata gccttacctg agagagatgt cgaaaaagcc
     6841 cttactcttc ttaaagagag tggtgaaaat gcgtggctta tcggtaaaat tgaagactta
     6901 ggcaccggta gcgagcaagt tgaaatccgc taatttgcaa aataaatgct aaaattgacc
     6961 gcttgtagta actgatctgc aaacggttaa atttttattg aggaatgcaa atgcaactat
     7021 tttggcctat ctactttgtc ttaatgaacc tagctgcctt atatttaatg tatagtgaca
     7081 aacaaaaagc gatcaaaaaa gaatggcgag tgccagaaat tactctcctc tctttttgcc
     7141 tcttaggtgg ggctatcggt gtttacctag gtatgaaata ctttcgccat aaaacccaac
     7201 attggtattt ccacgtcgct gttgtgcttt ctacttttgc ttggctgatt ggcttgcctt
     7261 acttttattt agctttatca tcatattaat ttatcattac tttttttaat ccgaaagccc
     7321 aatttatatc ctagtccgct cacaaaaaat ttgatttaga tcgcattatt tgtctattaa
     7381 aatcgttatt atctaccaaa atcgattgtc atttatttta aggagatgaa tatgtcagat
     7441 gtgcgtattg aacacgattt acttggtgaa agagaagtac ctaatagtgc ttattggggc
     7501 attcatacat taagagcgat tgagaatttt aatatctcaa atgtcaccat ttctgatgta
     7561 cctgaatttg tgcgtggcat ggtaatggta aaaaaagcta ctgcgttggc aaatggtgaa
     7621 ttgggtgcaa tcccgaaaaa aattgccaaa gctattgtgg cagcctgtga tgaagtatta
     7681 gtgaaaggac gctgtctcga tcaattccct tcagatgttt atcaaggtgg agccggcaca
     7741 tcggtgaata tgaataccaa tgaagtgatt gccaaccttg cattagaaat tttgggcgag
     7801 gcaaaaggta aatatgatat tgtcaatcca atggatcacg ttaatgcaag ccaatccacc
     7861 aatgatgcct atccaaccgg tttccgcatc gcggtttata acagtatcac taaattaatc
     7921 aataaaatca cttacctaca aactgcattt gaaaataaag ccactgagtt ttccgatgta
     7981 ctaaaaatgg gaagaacgca gttacaagat gccgtgccaa tgacgctcgg tcaggaattt
     8041 aaagcttttg ccgtattatt agatgaagag gttcgtaacc ttaaacggac ggcagaattg
     8101 cttcttgaag tgaatttagg cgcaaccgct atcggtactg gtctgaatac gccagagggt
     8161 tactctactc ttgcagttaa atatttagcc gaagtcacgc aactgccttg ctcactgtct
     8221 gaaaatctca ttgaggcaac atctgactgt ggtgcttatg taatggttca cggtgcatta
     8281 aaacgcaccg ccgtcaaact ttctaaagtg tgtaatgact tacgattact ttcatcaggc
     8341 ccacgtgccg gcttaaatga aattaatctt ccggaattac aagccggctc atctattatg
     8401 ccggcaaaag taaaccctgt tgtacctgaa gtcgtaaacc aagtgtgctt taaagtgatt
     8461 ggtaacgaca ccaccgttac ttttgcagca gaggccggtc aattacagct taacgtaatg
     8521 gagccggtta tcgggcaagc aatgtttgaa tctatcgagc tgctttccaa tgcatgtatt
     8581 aatctacgtg ataaatgtat tgacggtatt accgctaacc gtgacatctg ccaaaactat
     8641 gtgttcagct ctattggtat tgtgacctac ctcaatccat ttatcggcca ccataatggt
     8701 gatattgtcg gcaaaatttg tgcaaccacc ggcaaaagtg tacgagaagt tgtgcttgag
     8761 cgaggcttac tcaccgaaga acaactcgat gatattctct cgattgagaa cttaatgaat
     8821 ccgacttata aagccaaacg ctttaccgaa gaggaataat cattgtttac aagcggtctg
     8881 attctggcaa aaaattgcaa atttttacca aaatcagacc gcttgttatc ccattatttc
     8941 ttatttgctt tcagcatcgc cattaaattt gcaatatttg cagaagattt tgcctctttt
     9001 tcctgttcgg tcattcttgg cttttcactt tcccactcca aatcatcttg tggcaattcc
     9061 agtaaaaacc ggctcggctc aggtttgatg atttcgccat attggcgacg ttctttacac
     9121 agtgaaaacg tgagcgtttg ttgggctcgg gtaatgccga cataagccaa acgccgctct
     9181 tcttctacat tgtcctcatc aaggctggtt tggtgtggca gaatcccctc ttccataccg
     9241 attaagaaaa cgtgcggaaa ttccaaccct ttcgaggcgt gaagagtcat tagttgaact
     9301 tgatcggctt catcgtcctc ttcgccacgt tcgagcatat ctcgcaaggt taaacggttt
     9361 accacttgca ccaaattcat cggaggctct atttcatcgc cctctaacat cgcttgcacc
     9421 cattcaaaca aggtctgcac attacggctt tgcatttccg ctacttttgg gttcgccgcc
     9481 atttcataca aataggcttc atactgaatt ttcgccaaca gatcttttac tgcttcaatc
     9541 ggatcggatc tatcggcttg atcggcaatt tccacaatcc agcgggcaaa atcctgtaag
     9601 gcttggtaag gctttggcgt gaggcgttgt atgagttcaa aatcaaaaat cgcctcaaac
     9661 aaactgacct gcttttcatt agcaagtatg cctaattttt ctagcgttgc cgccccaatt
     9721 tcccgtttcg gcgtattcac aatacgcaag aaagctgcat catcgtcttg attcactacc
     9781 aaccgcagat acgccatcat atctttgatt tccgctcgag caaaaaatga cgtaccaccc
     9841 gaaattttat acggaatgcg gttttgcatt agcaattttt ccaacaagcg ggattgatgg
     9901 ttgcctcgat acaaaattgc ataatctttg tatttggtct ttttcgagaa acgatgggca
     9961 atcagctcgc ccacaatgcg ttccgcttcg tgttcctcat tttttgcctc aatgacctgt
    10021 aatttttccc cttgccccag ttctgaaaac agccgcttgc taaacaagtg atcgttatta
    10081 tcaatcagaa tattggcaca atgcagaatc cgctggctgg aacggtaatt ttgctcaagt
    10141 ttaatcactt gcaaatcaaa atcttcccgt aaccgttgca tattttcagg cttcgcccca
    10201 cgccaagagt aaattgattg gtcatcatcg cccacgaccg taaagtttgg ctcgttgccg
    10261 acaatcagct taattaactc atattggctg gtgttggtgt cttgatattc atcaactaac
    10321 aaatagcgaa ttttctgttg ccaacgagat tttgcttcgg gaaattggcg gaacagcaac
    10381 accggcaaca taatcaaatc atcaaaatcc aacgcattat aagccttaag ttgattctgg
    10441 tagagctgat aaaaatgtga aaacactcgc tcacgttcat cacgcacacg agtaagtacc
    10501 ctttcaggcg aaagcaaatc gtttttccag ttagaaatag tggcaattaa ggctttcagc
    10561 agatccttat cttccgtcac attttcaggc aataaatgtt tcagcaaagc aatttgatcc
    10621 tgctcatcaa acaaggtcat acccgattta aaaccgagca atttatattc gcgttttaaa
    10681 atttcaaacc caagtgtgtg aaaagtcgaa atggttaaac ctttgctgtt ttctttgccg
    10741 atagaatgat ccacccgttc acgcatttcc cgagcagctt tgttggtaaa ggttactgct
    10801 gcaatctgct taggcgaata gccacaatgt gcaattaaat gtgcgatttt attaataatc
    10861 acccgagtct tacccgagcc ggcaccagcc agcacaaggc aagccccttt ggtatattct
    10921 actgcttgtt gttgttgaga attgagtttc attgaatcta ttcaaaagta attttgacaa
    10981 ctattttaac ggaaagaaaa caagcggtcg aattttcagg aaaatttgca aaaaactttg
    11041 aaaatccgac cgcttgttca tcagagaaaa atccccaaaa cgcaaatttt tcctttcaat
    11101 cttgcccctt tagctatatc attataagtt gattttttag gggcaaatgc ctgattttat
    11161 aactcctttt ctttttaatt taagggaaaa aaacagtaga tggaaaaagt agaacagaaa
    11221 gctatcattg agcttaaaaa tgtaacaaaa agctacggta gtaaaacgat tcttaaaaat
    11281 ttaaatttaa ccattaacga tggtgaattt ttaacaattt taggcccttc cggttgtggt
    11341 aaaacaactg cgttacgttt aattgccggt tttgaagatt taactgaagg ttcaattatt
    11401 cttgatgggc aagatgtttc taacgttccg gcggagcaac gcccggtaaa caccgtattc
    11461 caaagctatg cccttttccc acatatgacg atttttgaaa acgtggcttt cgggctgcgt
    11521 atgcaaaaag tgccaaatgc tgaaattgag ccacgtgtga tggaagcatt acgaatggtg
    11581 cgtttagaag accgcgctca acaaaaaccg gctcagcttt ccggtggtca gcaacaacgt
    11641 attgcaattg cccgtgcggt ggtaaataaa ccgaaagtat tgttgttaga cgaatcgctt
    11701 tctgcgttag attataaatt gcgtaaagag atgcaaaatg aattaaaagc attgcaacgt
    11761 caattaggca ttacctttat ttttgtgacc cacgatcagg aagaagcctt aaccatgtct
    11821 gatcgtatta tcgtaatgaa cgctggtaaa atcgctcaag acggtacacc acgtgagatt
    11881 tacgaagagc caagtaactt atttgtagct cgtttcatcg gcgaaattaa cgtttttgat
    11941 gcaactgtaa ttgagcgtgt tgatgcaact aatgtaaaag caaacgtaga aggtcgcatt
    12001 tgcaatatca aagtggaaga tttagaggtt cagccgaacc aaaaattaaa agtattgtta
    12061 cgccctgaag atattgtgat tgaggagtta gacgaaaacg aaagctcgaa agcaattatc
    12121 ggtcatatta tcgaccgtaa ctacaaaggt atgacattag agtctagcgt gagattagat
    12181 cataacggta tgaatgtatt ggttagcgag ttcttcaatg aagacgatcc gaacattgac
    12241 cacgaagtgg gacaaaaagt tgctcttact tggtatgaag gctgggaggt tgtgttaaac
    12301 gatgaaggcg aataacaaat tccaaaatgg tgcggtcgca actattttcg gttggttatt
    12361 acttttcgta ttagtgccga acgtactcgt tttaatcatc agcttcttaa cagaaaaccg
    12421 tcaaagtcag tattttgtgg actttgcatt tagttttgat gcttataaat cactgtttaa
    12481 tgacacctat gcagcagtat tgtggaactc gctatatatg gcgggcattg ccaccttttt
    12541 ctgtttgatc atcggctatc cctttgcatt ttttattgcc aaaatgccgg aaagaattcg
    12601 tccgttttta ctgtttttag tagttctacc gttttggact aactcactga ttcgtattta
    12661 tggcattaag attttcttag gcgtaaaagg ctttttaaat gaaagcttga tggctttagg
    12721 tataatcagc gagccaatgc gtttattaaa ctcagaaatt gcgattatta ttggtttagt
    12781 ttacattcta ttacctttta tgattctacc gctttattca tcaatcgaga aaattgataa
    12841 ccgcttacta gaagctgcaa aagacttagg tgcaaacgcc ttccagcgtt ttattaaagt
    12901 gactattcca ttaacaatgc cgggcattgt cgcaggttgt ttattagtat tacttccggc
    12961 aatgggtatg ttctatgtgg cagatttatt aggcggcggt aaaacaccgt tagtaggtaa
    13021 cattattaag agcttgttct taaataccaa ccaatttgca ttaggttcag cagtaagtat
    13081 tgcattaact atcttaatgg cattaatgct ttatgtttac tatcgtgcaa acaaattatt
    13141 aaataaaaag gtggagcttg aataatgaaa aagatgttta gaaacctttt tatgttggtt
    13201 gtttttgcat atctctatat tccaattatc attttagtga tcaactcctt taatgaagac
    13261 cgctttggat taaattggaa aggctttact tggaattggt atgagcgttt attcagtaat
    13321 gatacgcttg tacaagcaac attgaactca ttaactattg cgttctttgc ggcaacattc
    13381 tcaaccattt taggggcatt aacctctatt gcgctttacc gttaccgttt ccgtggtaaa
    13441 aagctagtgg gcggaatgtt attcgtggta atgatgtcgc ctgatatcgt aatggcggta
    13501 tctatgctcg tattatttat gatcttaggt atcaagctag gctttatttc gctattaatt
    13561 gcacacatta ctttctgttt gccttatgtg gtaattgcaa tttcctcacg cctcagcgat
    13621 tttgacggca aaatgctaga agctgcaaaa gacctaggtg caagtgaagt gactattttg
    13681 cgtaaaatta ttttaccact ggcattacct tcgattattt caggctggtt gttaagcttt
    13741 acgatttcgt tagatgacgt tattgtttca tctttcgtga caagcccaag ctacgaagtt
    13801 ttaccactga aaattttctc attggttaaa acaggtgtaa cacctgaagt gaatgcacta
    13861 gcaactatta tgattatttt ctcattaaca ttagtggtat taagccagtt agtagtgaag
    13921 aaaaaagtct aaaaatcact aaaaatcgta tacataaatc agcactttaa agagtattaa
    13981 agtgccgatt tttttacaaa aataagctaa aaaaacaacc gcttaagaat aatgaattct
    14041 tataaaaata gccataacta atcaatatct ttacaaagcc gttaatttac gctattaatt
    14101 gcctaccaat ataactaaaa agagtggtgc ttatgctgaa gtttattttt aaacgtcttc
    14161 ttgaggcgat tccaacccta ctaattctga ttaccttttc ctttttcctg atgcggcttg
    14221 ccccgggtag cccatttacc agtgagaaag cctatccgcc gaaagtaatg gcaaatatcg
    14281 aggcaaaata tcacctgaat gaaccgctgt ataagcagta ttttctttat atggaaaatc
    14341 tgtcaaaagg cgatttcggt ccatctttta aatataaaga tcaatcggta aataatctga
    14401 ttgcttctgc gtttcctgtt tcgataaaac tgggcattac tgcctttatt tttgcggtgc
    14461 ttatcggttt aaccgcagga actatcgctg cacttaagca aaatagtaaa tgggattatt
    14521 tagtgatggg cttttcaatg acgggtatta ttatgccgag ctttgtcttt gctccgctgt
    14581 tggtactcat gtttgcgatt tatctcaaat ggttgcctgc cggtggttgg aacggtggtc
    14641 agctctacta tatggtttta cctgttctct ctttaaccat tagctatgtt gccggcattg
    14701 cccgtattac tcgtggttca atgattgagg tgttgcactc taattttatt cgcaccgcta
    14761 aggcaaaagg cttgccaatg cgtaaaatta ttttcaaaca cgctttgcgt ccggcattgt
    14821 tgcctgtgat cacttattta ggcccggctt ttgttggcat tattaccggc tcgatggtaa
    14881 ttgaaagcgt gtttggctta ccgggaatcg ggcagttgtt cgtaaacgga gcattaaatc
    14941 gtgactattc attagtatta agcctaacca ttttggtagg tacgctcacc attttattta
    15001 atgcgattgt tgatatttta tatgcggtca tcgacccgaa aattcgttat tcataggagt
    15061 aaagcggaaa atgcaaaatt cttcaaaaaa tcaaccgctt gttgaggtta cctcaaccat
    15121 tcaaggacga agtttatggc aagatgcacg ccgccgtttt ttccgaaata aggcggctgt
    15181 ggcaagtttg attattcttt gttgtattat tctttttatt acctttgccc caatgctaat
    15241 gccgtttagc tatgaagata ccgattggaa tatgatgggg attgctccgg attttgaatc
    15301 tcaacactat ttcggtactg attcttcagg gcgtgatttg ctggttcgag ttgcaatcgg
    15361 tggacgcatt tcgttaatgg tagggattgc cggtgcatta attgcggtgc ttatcggtac
    15421 aatctatgga gcaatttccg gttatttcgg cggtaaagtc gatatggtga tgatgcgttt
    15481 tctggaaatt ttaagctcat tcccatttat gttcttcgta attttgctgg ttacgttctt
    15541 cgggcagaat attttcctga ttttcattgc aatcggtatg gtagcgtggc tgaaccttgc
    15601 ccgtattgta cgtggtcaaa ctctttcgct taaaaataaa gagtttgtgg aagcagcgat
    15661 tgtgtgtggc gtttcccgcc gtcaaattat ttggcgacac attgtgccta acgttttagg
    15721 cttagtggtg gtttatgcct cccttgaagt accatctctg attttgtttg aatcgttctt
    15781 aagtttctta gggcttggta cacaagagcc gatgagcagt tggggggcat tattaagcga
    15841 cggagcggca caaatggaaa cctcgccttg gctactggct ttccctgcct ttttcttatg
    15901 tttaacactg ttttgtttta attttattgg tgatggcttg cgtgatgccc tcgatccaaa
    15961 agataaatag aggtttagaa tggaattatt agcagtaaat aatctcgatg tttatttaaa
    16021 aacggacgaa gaattagttc acgcagtgcg taatgtctcg ttctcgattg aaaaagggca
    16081 aacccttgcg attgtggggg aatcgggttc gggtaaatcc gttacttcaa tggcaattat
    16141 gcagttattg cctaaaaata tcgtctcatt cggtgaacgc tcaagcatca tttttgaaga
    16201 acaaaacttg cttactctat ctgaaaaagc aatgcaaaca gttcgaggtg atcgcatcgg
    16261 tatgattttc caagagccaa tgacctcact caatccttat atgccgattg gcaagcaggt
    16321 tgctgagacg gttcaaaccc ataatccgca aatcagcaat gccgaagcgg aaaaaatggt
    16381 gttggaaact ttgcaaaaag taaaaattcc cgatgcggaa aagaaaatga tttgctatcc
    16441 gcacgagttt tccggcggtc agctacaacg aattatgatt gcaatggcaa ttatcaacaa
    16501 acccgattta ttgattgccg atgagccgac caccgccctg gatgtcacaa cccaagcgga
    16561 aattttggat ttaatgcacg acttacagcg tgatatggga atggcgatta tcttaatttc
    16621 ccacgattta cgcttggtgc ataaatacag tgacttagtc tgcgtaatgc aaaacggcga
    16681 aattattgag cgaggcgaaa cggaaactgt gttcaccaat ccgcaacatc cttatacggt
    16741 ggagctactt accccgattc caactaattt aaaaggggaa ttaccgccaa atgcaccaac
    16801 cttgcttcag ggtgagcata ttaaggtaga ttatatttta aaacgctcgc tattcggtaa
    16861 gccgaaaaag gtgtttaatg cgttaaaaga tatttcattg cacttgaaaa ccggcgaaac
    16921 gctcggcatt gtaggcgaat ccggctccgg gaaatctacc cttgggcgtg ccattatgca
    16981 gattttagat tatcgcggca atattacatt tgcaggtaag acactggcta atctgaataa
    17041 agccgaacgt caagccctga aaaaagatat gcaaatggtg ttccaagacc cgttcaactc
    17101 actttctcct cgcttaacag ttggggaaat tattgctgag ggcttaaccg tacattgccc
    17161 tcaaatgagc aaaaccgaac ggcgggaaaa agtaatgaaa atgttggaag aggttaatct
    17221 caaccctgca atgattaacc gctatccgca cgaattttcg ggcggacaac gccagcggat
    17281 tgcgattgca agggcaatca ttttagagcc gaaatttgta ttgcttgatg agccaacctc
    17341 agcacttgac cgctctactc agatcactgt aattgaattg cttaaccgct tacagaaaaa
    17401 atacggctta agttatattt tcatcagcca cgatttggcg gtcatcaagg cacttagcga
    17461 ccgagtgatt gtaatgagcc aaggtgagat tgtagaaagc ggtgatgtca ctcaaatttt
    17521 tgaaaatcca caacaagaat acaccaagcg gttgatttta gcctctaatt tgtaacccct
    17581 tccacaaggt cttattctac caaaaaacaa gaccttgtca ttttatgcta gcaaaacatt
    17641 gtaattttga tattttattt caattttttg aaaaaatatg gaactttata
//
LOCUS       DANLXQ010000036        17625 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.036, whole genome shotgun sequence.
ACCESSION   DANLXQ010000036 DANLXQ010000000
VERSION     DANLXQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17625
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.036"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            262..1626
                     /locus_tag="PXD94_000929"
     CDS             262..1626
                     /locus_tag="PXD94_000929"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL6285813.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMR
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            1716..3251
                     /gene="ddc"
                     /locus_tag="PXD94_000930"
     CDS             1716..3251
                     /gene="ddc"
                     /locus_tag="PXD94_000930"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL6285814.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSLEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            3354..3716
                     /locus_tag="PXD94_000931"
     CDS             3354..3716
                     /locus_tag="PXD94_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL6285815.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQVL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSEGNKVRLYGE
                     IDKGFNKTELDVKTLTVL"
     gene            complement(3806..4393)
                     /locus_tag="PXD94_000932"
     CDS             complement(3806..4393)
                     /locus_tag="PXD94_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6285816.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            4569..5117
                     /gene="napF"
                     /locus_tag="PXD94_000933"
     CDS             4569..5117
                     /gene="napF"
                     /locus_tag="PXD94_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL6285817.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            5110..5376
                     /locus_tag="PXD94_000934"
     CDS             5110..5376
                     /locus_tag="PXD94_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL6285818.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            5415..7913
                     /gene="napA"
                     /locus_tag="PXD94_000935"
     CDS             5415..7913
                     /gene="napA"
                     /locus_tag="PXD94_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL6285819.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEAHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVENSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEADHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            8002..8907
                     /gene="napG"
                     /locus_tag="PXD94_000936"
     CDS             8002..8907
                     /gene="napG"
                     /locus_tag="PXD94_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL6285820.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGMILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCLDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            8907..9785
                     /gene="napH"
                     /locus_tag="PXD94_000937"
     CDS             8907..9785
                     /gene="napH"
                     /locus_tag="PXD94_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL6285821.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGATLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTVILSKDCITCGRCIDVCAENV
                     FAFGMRFQGSIDVKNI"
     gene            9831..10259
                     /locus_tag="PXD94_000938"
     CDS             9831..10259
                     /locus_tag="PXD94_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL6285822.1"
                     /translation="MRKYLAFILAVMSGFAMAEAPKLSGSIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            10270..10878
                     /locus_tag="PXD94_000939"
     CDS             10270..10878
                     /locus_tag="PXD94_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL6285823.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FTGKVDTKEKFEAHRGEMAEREWARMKANSSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(10940..11759)
                     /gene="cpdA"
                     /locus_tag="PXD94_000940"
                     /pseudo
     CDS             complement(10940..11759)
                     /gene="cpdA"
                     /locus_tag="PXD94_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
     gene            12064..12198
                     /locus_tag="PXD94_000941"
     CDS             12064..12198
                     /locus_tag="PXD94_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL6285824.1"
                     /translation="MRSELLKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA
                     "
     gene            12202..13365
                     /gene="torC"
                     /locus_tag="PXD94_000942"
     CDS             12202..13365
                     /gene="torC"
                     /locus_tag="PXD94_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL6285825.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREAW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMGEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPNQGKSYYTKGNAELFLD
                     EGLSQAIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            13465..15930
                     /gene="torA"
                     /locus_tag="PXD94_000943"
     CDS             13465..15930
                     /gene="torA"
                     /locus_tag="PXD94_000943"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL6285826.1"
                     /translation="MQQSRRQFLKNMSVMAAAVTMPNFLVPRNVFASEDLSQWKVSGS
                     HWGAMRAKIENGRVAEIKPFEFDKHPTEMLNGIKGLIYSDSRIRYPMVRLDWLKNRHN
                     SNTAQRGDNRFVRVTWDEALDLFYQELERVQKEYGPWALHTANVGWRSTGQFHSCGNH
                     MIRAIAMHGNSVGTAGDYSTGAGQTILPYVLGSTEVYSQGTSWEIILKESENIIFWAS
                     DPVKNLQVGWNCETHEAYAYLEKLKEKVAAKGVNVICVDPVKSKTQNFLGCEQQYINP
                     QTDVPFMLALAHTLYTENLYDKKFLDMYTVGFEKFLPYLLGETEDKVAKTPEWAAEIC
                     GISADKIREFARMLAGKRTQFIFGWAIQRQQHGEQPYWMGAVLASMLGQIGLEGGGIS
                     YAHHYSSIGIPESGAAMPGAFPLNIDEGQTPKYDNKDYKGYSDTIPVARMTDSLLHAG
                     ETIDYNGKKVTYAPFKMAIFTGCNQWHRHSERNKMKQAFQKLETIVSINYSWTATCRF
                     SDIVLPACTPFERNDIDAYGSYSNRGVIAMHKLVDPLYDSRSDFEIFRDLCRRFGKEK
                     EYSRGMDEMQWVEKLYKDCRRENRGKFEMPEFAEFWQKGYVLFPEGKPWVRHADFRED
                     PELHALGTPSGFIEIYSNKIASFGYDDCKGHPMWFEKAERSHGGKNSDKFPYWLQSVH
                     PDKRLHSQLCESKELRETYSVQGREPLYINPQDAAKHGIQDGDLVRVFNDRGQAIVGA
                     VLSDNFPTGVVRLQEGAWYSPLDESVGAIDTYGDPNTMTLDIGTSKLAQAVSANTCLV
                     NIEKFIGNAPAPNGFNGAIEVTA"
     gene            15930..16520
                     /gene="torD"
                     /locus_tag="PXD94_000944"
     CDS             15930..16520
                     /gene="torD"
                     /locus_tag="PXD94_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL6285827.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
     gene            16660..17349
                     /locus_tag="PXD94_000945"
     CDS             16660..17349
                     /locus_tag="PXD94_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL6285828.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
BASE COUNT         5126 a         3588 c         4126 g         4785 t
ORIGIN      
        1 ataaaagctt cccttttcaa taaaaaagag tatcatacgc tctcttaaac gtaaccctac
       61 atttaagtct gtttccaacc tatcccaatt tctttattcc ttaggggcat atagggtaaa
      121 aacaaaaaag ctgaaacgct ttttttaata agtagcgagt caaatgcccc atggcatcta
      181 ttcttttagg aatagaaagc ttaggctttc cgcaaggcac ccgacttatt tttttcacct
      241 cttgtagaag gtttattttc aatgacaatt aaaactccta ttcaagccgt tcttgcttca
      301 aaccaatatt ttttagatcg ccaagatgca atggaatcaa acgtgcgtag ctacccacgt
      361 aaattacctt ttgcttacca aaaagcacaa ggcgtttggg taactgatgt tgaaggcaat
      421 gaatacctcg atttcctagc aggtgcagga acgcttgcat taggtcataa ccaccctaaa
      481 ttaatccaag cagttaaaga tgtattagac agtggtttac ctttacactc gttagatatt
      541 acgactccgt taaaagatgc ttttacggaa gaattactct ctttctttcc aaaagataaa
      601 tatatcctcc aatttactgg cccgtctggt gcagatgcta acgaagcggc aattaaatta
      661 gccaaaacct acactggtcg tggcaacgta attgctttct ccggtggttt ccacggaatg
      721 acccatggtg cgttatcgct caccggtaat ttaagtgcga aaaatgcagt acaaggctta
      781 atgccgggcg tacagtttat gccttatccg cacgaatacc gctgtccgtt tggcattggt
      841 ggcgaagccg gtgcaaaagc agttgaacat tattttgaga actttattga agatgttgaa
      901 agtggtgtgg taaaaccggc agcggtgatc cttgaagcca ttcaaggtga aggcggtgtg
      961 gttccggctc caatcagctt cttacaaaaa gtgcgtgaag tcactcaaaa acacggcatc
     1021 ttaatgattg tggacgaagt tcaagctggt ttctgccgtt cagggaaaat gtttgccttt
     1081 gaacacgcag gcattgagcc ggatatcgta gtaatgtcaa aagcgattgg tggtagctta
     1141 ccgcttgcgg tattagcgat caaaaaagag tttgacgctt ggcaaccggc aggtcatacc
     1201 ggtacattcc gtggtaacca attagcaatg gcaaccggct atgtatcgct taaaattatg
     1261 cgtgaagaga atttagcgaa aaacgcacaa gagcgtggcg aatacttaac taacgcatta
     1321 cgtgaattaa gcaaagaatt accttgtatt ggcaatgtgc gtggtaaagg cttaatgatg
     1381 ggaattgata ttgttgatga acgtcagcca aaagatgcaa caggtgcata cccgcgtgat
     1441 tgcgatttag ccgttgcaat tcaaaaatac tgctttaaaa acaaattgtt attagagcgt
     1501 ggcggacgtg gcggtaatgt ggtgcgtgtt ctttgtgccg tgaatattac ccaagcagaa
     1561 tgtgaagagt ttattaaacg ctttaaacaa tctgttgctg aagcagtgaa agcggtgcgt
     1621 ggttaatagt tagaatggta ggagcgaaac atttttcgcc ctaatgtaaa aaggggcgaa
     1681 tatttattcg cccctacacc aataggatag aaataatgtc aaacctagaa aaacacagac
     1741 aatccctgtt ttgtagcgat ccaaaatcga ttgcggatta tgaatcggca atgaataacg
     1801 ctgtgaaagc ggtttcagac tggctgaaag aagaaaaaat gtacaccggt ggctcaatta
     1861 aagagttgcg tgccaaaatc aacggtttta acccaaccca agctggtgtg ggcgtgcaaa
     1921 aatcactgga gcatttggtg gaaattttct taaagccaag cctcaaagtt caccaccctc
     1981 attctttggc ccacttacat tgcccgacaa tggtaaccag ccaaattgca gaagtgttaa
     2041 ttaatgccac caaccaatca atggactctt gggatcaaag cccggcaggt tcgattatgg
     2101 aagagcattt aattgactgg ctacgtcaaa aagtcggcta tggcaaagga acttcaggag
     2161 tctttacctc aggtggtacg caatcaaacc tgatgggcgt attattggct cgtgactggg
     2221 cgatagcgaa ccattggaaa aaggcagacg gttcagagtg gtcggtgcaa gccgacggta
     2281 ttcctgccga tgcaatgcaa aaagtgaaag tggtttgttc cgaaaacgct cacttctcgg
     2341 tgcagaaaaa tatggcaatg atgggaatgg gtttccaatc ggtagtgact gtaccgtcaa
     2401 atgccaatgc acaaatggac gttgaagctc ttgcgaaaac gttggcggat ttaaaagccg
     2461 aaggcaaaat tgtggcttgt atcgtcgcaa ccgcagggac gaccgatgcc ggtgcgattg
     2521 atgatttaaa agcgatccgt aaattagccg atgaacatca ggcttgggtt cacgttgatg
     2581 ctgcttgggg tggtgcatta ttactgtcga aagatttccg ccacttctta gacggcattg
     2641 aattaaccga ttccattacc cttgatttcc acaagcattt cttccaatca atttcgtgtg
     2701 gggcattctt gctaaaagat gaagcgaatt accgctttat tgattacaag gcagactacc
     2761 tgaattcaga atatgacgaa gaacacggcg tgccgaattt agtgtcaaaa tcattgcaaa
     2821 ctacccgccg ttttgatgcg ttaaaattat ggtttaccgt tgaagccttg ggtgaggagc
     2881 tatacgcttc aatgatcgac cacggggttt atctttctaa agaagtggaa gcctacatta
     2941 atgccaccga tggcttggaa atgttagtgc cggcacaatt tgcctcagtc ttattccgtg
     3001 tcgtaccaca aggttacccg gcagaatttg tggatacctt aaaccaaaat gtggcagatg
     3061 agttatttgc tcgtggcgaa gctaacattg gtgtgaccaa agtcggtgag catcaatcac
     3121 tgaaaatgac tactttaagc ccgattgcaa cgcttgaaaa cgtaagagca ttattagctc
     3181 aggtgttggc agaagcagat cggattaaag attcgattgt aaatggaact tatgttccgc
     3241 caattgatta aactgcgtta aaaataaaaa ggtgttttcg acatttattt gtgttttaat
     3301 gtttgcattc ggtagtcttt atcgaatgca aattttttta acaaggagtt tttatgaaaa
     3361 aagtcctggg tttaacattg atggcaatgg cgttatcggc gtgtaattca actgcttcca
     3421 acggttcttc acaagcaaat acaggtgttg caccggcgaa tgtgattagc gtgaaacagg
     3481 tgttagcggc agcagatgac agctacgtga cggtagaagg cacactctta aacaaagtcg
     3541 gtgacgaaac ttacttattt aaagatgcga cagggcaaat ccgtgttgag attgacgatg
     3601 aagtatgggc aggtcagcac gttggtagcg aaggcaataa agtccgttta tacggagaga
     3661 ttgacaaagg tttcaataaa accgagttag atgttaaaac actcactgtt ctgtagaaga
     3721 tcaaacctta atttggttcc ccgccttgtt tggcggggtt tttacgttaa ttaatcaaac
     3781 catccttttg caaaaaaacg caaaattata ccgcttgttc cttcaatatt gtgctacaag
     3841 atgctgtgat ttgctgcaaa atagcggtgt aatcagtctc ttttaaacca atattaccga
     3901 aatagtgcat ttttacatca cgaatcccac agaactcaaa cagccctttt cccagcgtat
     3961 gttcaaatgc tgctaaaaag ccttttcgtt catatttttc attggaatta ccgagcgtta
     4021 caaattgttg catttttttg ccggtcagca agccgatact ttcaatttcc ccgttacgat
     4081 aggcaaaatc ataacttaac accctgtcta aatagccttt taaaattgcc ggaaagccca
     4141 tccaccaaag cgggtaaatt aacgtaatca catcagcttc aagccaatat tgatgctcaa
     4201 ttgccacatc tgcttcatag tgtttactga gtgtgccttg aaattccgcc cagtctaata
     4261 tcggcttgaa gttgagttgg tagagatctc gtacaaccac cttatttgga acggaagctg
     4321 ctatcgtttt ttccaaaatc gcatggttaa agctggaggg attcgggtgt gcgtaaataa
     4381 tcaggtgatt catagcgttt tcctttactg tgtcgaggca gctactagaa gattttagcg
     4441 aaactttctt tgatgaaaat caatattttg gcgaatatac cggcaaatac cccgtttgag
     4501 gaatgaacgg ttttaagtga acattagata atttagccta ttatagtgat gagtaaaaag
     4561 gtgttttctt gactgattat cctaatcaat cacttcctcg ccgtagcttt ttgcgagggc
     4621 aatttttaga ctcgttaaaa agccaaaaag tcagtcagca agggtatgag ccgattcgtc
     4681 cgccttgggc taatttagca aattttttag aaaaatgcac cgcttgcggt aactgcattc
     4741 aggcttgcga aacccagatt attgtaaaag gtgctgcggg ctaccctgaa attgatttta
     4801 gccgtggtga atgcactttt tgtgaaaaat gtgtgcattc ttgcgaggca gaggtattcc
     4861 gagcagtttc agaacagcct tggagccata aaattgagat tcaagccggc tgtttattaa
     4921 agctcggaac ggaatgccga agctgtggcg atagctgtga gatgagagtg attcgtttcc
     4981 gcccgagttt aggtggcatt gccgaaatgg tattaaattt ggaaagctgt aatggctgtg
     5041 gggcttgttt gagcgtttgt ccgacaagtg cgataaaaat tcagccatta caaacacagg
     5101 caaacagaga tgagtgaaaa attatccgaa caggaaaact ggtatgtgtg cagtctcgtg
     5161 gtgcaagcaa aaccggaaaa attggaagaa gtaaaagccg agattctgaa gattcctcat
     5221 accgaaattc acggtgagaa aacagatgaa ggcaaattgg tcgtgacgat tgaaagcaat
     5281 gtgcatttag ccctttccga tcgaatcgat caagtaagag atattaaagg cgtgattgtg
     5341 gtgtctttaa tttctaatta cattgatgaa cagtgattaa tgattatttt tgaagccaaa
     5401 cgtgggacat agttatggaa ctcaatcgta gagattttat gaaagcgaac gccgctgtgg
     5461 cagctgctgc agccgcagga attatgattc ctgtgaaaaa cgttcaggca gatgacaccg
     5521 gtattcgttg ggataaagcc ccttgtcgtt actgtggtac aggttgtagc gtgctagtcg
     5581 gtactaaaga cggtaaagta gtggcaaccc aaggtgaccc ggatgcagaa gtaaaccgtg
     5641 gtttaaactg tattaaaggt tatttcttat ccaaaatgat gtacggtgca gaccgtatgc
     5701 aagagccaat gttgcgtatg aaagacggta agttcgacaa gaacggcgat ttcacgccgg
     5761 tttcttggga tcaagccttt agcattatgg cggagaagat taaaaccact attaaaaatg
     5821 acggcccgaa tgctgtaggt atgttctcat caggtcaaac aaccattttt gaaggttatg
     5881 cgaaagtgaa actttggaaa ggtggtttcc gttcaaatac gattgacccg aatgcccgcc
     5941 actgtatggc gtccgctgcg gttgccttta tgcgtacctt tggtatggac gagccgatgg
     6001 gctgctataa cgacatcgaa aaaaccgatg cgttcgtgct ttggggttca aatatggcag
     6061 aaatgcaccc gattttatgg tctcgtattt ctgatcgccg tttatcagac aataaagtga
     6121 aagtggtggt aatgtccact tacgaacacc gttcgtttga attagccgat acaccgatta
     6181 tcttcaaacc acactcagat ttagcaattt taaactacat tgcgaactac attatccaaa
     6241 acgataaagt aaattgggat tttgttaaca aacacaccaa atttaaacgg ggtgaaacca
     6301 atatcggtta cggtttacgt cctgaacaca aattacaaca aaataccaat gcgaaaactg
     6361 ccggtaaaat gtatgacagc gattttgaag agtttaagaa aatcgttgcc ccttatacat
     6421 tagaagaagc tcaccgtatt tcaggcgtgc cgaaagatca gcttgaaaca ttagcgaaat
     6481 tatatgcaga tcctaaccaa aaattagtgt cgttctggac gatgggcttt aaccaacata
     6541 cccgtggtgt gtgggtaaac catatgatgt acaacgtcca tttattaacc ggtaagatcg
     6601 cattagaagg ttgtgggccg ttctcactca cgggtcagcc gtccgcttgt ggtactgccc
     6661 gtgaagtggg tacatttatt caccgcttac cggcagatat ggtggtcaca aatccgaaac
     6721 accgtgagat tgtagagaat tcttggaaat taccggctgg agcaattcct gatgtaccgg
     6781 gctttcacgc tacagctcaa agccgtgcct taaaagacgg caaaatgaaa ttcttatggc
     6841 agatgtgtac caacaatatg cagggcggcc cgaatatcaa tgaagagatc ttcccgggct
     6901 ggcgtaatcc tgaaaccttt attgtggttt cagatccgta cccatctgtt tcagcagtgg
     6961 ctgctgactt aattcttcca acctgtatgt gggtggaaaa agaaggggct tacggtaacg
     7021 cagaacgccg tacccaattc tggcgtcagc aagttaaagg gccggcaaat gcccgttcag
     7081 atacttggca gttagtagag ttctctaaat acttcaaagt agaagaagta tggccggaag
     7141 agttattagc taaagcaccg gaattgcgtg gtaaaactct atacgaaatc ctttaccgca
     7201 acggcaaagt agatagctac caagtgccaa ctaacattcc gggctatatg aatgacgaag
     7261 cagatcactt cggttactac attcaaaaag gcttatttga agagtatgcg gaatttggcc
     7321 gtggtcacgg tcacgattta gccgactttg acacctacca caaagtacgt ggcttacgtt
     7381 ggccggtggt aaaagacgag aaaaccggcg aatacaaaga aactttatgg cgttaccgtg
     7441 aaggctatga cccatacgtt aaagccggtg aagaagtggc attctatggc aatgcggaca
     7501 aaaaagcagt gattttaggt gtgccatacg aaccacctgc ggaagcaccg gacgaagaat
     7561 atgacttatg gttctgtacc ggtcgtgtac tagaacattg gcacaccggt acgatgaccc
     7621 gccgtgtgcc ggagttacat aaagcattcc caaccaactt ggtttggatg cacccgaatg
     7681 atgcgaaaaa acgaggctta cgtcacggtg ataaagtgaa agtgatttca cgccgtggtg
     7741 aatatgtatc gaacttagat acccgtggac gtaataaatg tccggaaggt ttgatttact
     7801 cgactttctt cgatgccggt cagttaatca acaaagtgac acttgatgcg actgatccaa
     7861 tttcatttga aaccgacttc aaaaaatgtg cggttaaagt ggtgaaagct taattgtagg
     7921 ggcaaattat ttgcctcaga caagcggtca aatttgcaaa tttttttaca aaaacgaccg
     7981 cttacctaag aggaacccac aatgaaaatg gatccaaatc gtcgccagtt tttaaaaaat
     8041 gcaacccgta ccgcagccgg tgtttgcggt gtaggaatga ttttagggct acagcaacaa
     8101 caaagttttg cccgtcaagg ggtagcacta cgcccaccgg gtgcattgcc ggagcaagat
     8161 ttcctgagtg cttgcacccg ttgcggacaa tgtgtgcagg cttgtcctta cgatatgttg
     8221 catttggcat cgcttatttc accagtggaa gccggcacgc catactttat tgcacgggat
     8281 aaaccttgcg aaatgtgctt ggatattccc tgtatgaatg cttgcccaac cggggcatta
     8341 agcgaagagc taaaagacat tgacgatgca cgaatggggc ttgcggtgtt gctcgaccac
     8401 gaaacttgtc ttaactggca aggcttgcgt tgtgacgtgt gttaccgtgt ttgtccgtta
     8461 atcgacaaag cgattactct tgagaaagtg cataacgacc gtacctcgat tcacgccaaa
     8521 ctgattccga ccgtgcattc tgatgcttgc accggttgcg gtaaatgtga acaggcttgc
     8581 gtattggaag aggcagcgat caaagtgtta ccgatggatt tagccaaagg cttgctcggt
     8641 cgtcactatc gtttaggctg gcaagagaaa caaaatgccg gtaaatcgct gattgaagat
     8701 gttcacccag agggtttacg cccagcttat gatgccagaa tgccggaagg cttacacgag
     8761 ccggtgtatc agcatatgga agtaaaacct gatgtgaaag tggctacgcc aagtcgggcg
     8821 acacaaaact atgtgccgaa ctctacgacg gtggaagctc cggagcattt ccctgattta
     8881 gacttgaatc tcaagggggt gaaataatgg cagccaattc tccaaaaaat gcgggcttag
     8941 aggctcgtca gaaactaggt tggtggcacg cttaccgctt tttgatttta agacgcttaa
     9001 gccaactcag cattattttg atgtttttaa gcggcccact ttggaatgtc tggattttaa
     9061 aaggaaacta cagcggtagc ttattactag ataccgtgcc gatgacagac ccattaatga
     9121 ccgctgaagt gctggcaacc ggctatcgcc ctgaatggac ggtattggtt ggggcgttaa
     9181 ttatggtggc gttttacggc attgtcggca gcaagctgtt ttgtagttgg gtctgcccga
     9241 tgaatatggt aacagatact gctgcttggt taagacgcaa actcggtatt cgccaaactg
     9301 ctaaaattcc acgcaattta cgctatgtga ttttggcggt gattttagtc ggtagtgcga
     9361 tttccggaac actattgtgg gagtggatca acccggttgc ggctctcggt cgggtgtttg
     9421 tattcggttt aggggcaacg ctttggctgg ttttagtggt gttcttgttt gacttattgg
     9481 tggcagaaca tggctggtgt ggtcatcttt gcccgattgg tgcaacgtat gccttaattg
     9541 gggcgaaaag cctgattaaa gtcaaagtgg tagatcgtaa ccgttgcgat aattgtatgg
     9601 attgttacaa cgtttgtccg gaacctcaag tgttgcgtgt gccgctacac ggtaaaccgg
     9661 aggaaagcac ggttatctta tctaaagatt gcattacctg cggacgttgt attgacgttt
     9721 gtgccgaaaa tgtatttgcc ttcggaatgc gttttcaagg cagtattgat gtgaaaaata
     9781 tttaattttt atctttttga aaacaagaat tttaatggag tgaacctcaa atgagaaaat
     9841 atctcgcctt cattttagcc gtgatgtccg gctttgcaat ggcggaagcc cctaagttat
     9901 ccggatcaat tgatgagaca accgaaagtg ttgcaccggg atttcataac ccacaaaaag
     9961 atgtgggcac gattccaacc actttccctt tccaaccacc gcttgttcca catagtattc
    10021 gtggcttaca agtgagcaaa aacgtaaacc attgtttagg ttgccacgct attgaaccat
    10081 ctaaaaccac aggtgcgacc cgtttaccgc caagccattt taccgaccgt gatggcaagg
    10141 tgtacgaaaa cgaatctcct cgtcgttatt tctgtttgca atgccacgtg caacaaactg
    10201 atgtaaaccc gattattcaa aataaatttg aatctgtacg tgaatctcaa gctaaataag
    10261 gagtaagtaa tgttgagaag attttgtaac tggttacgta gcccaagcaa aatggcaatt
    10321 ggtgctgtaa tccttatttc tgcattaggt ggtattttcg cttggagtgg ttttaatgca
    10381 gggttagcaa tgacaaatac tgaagagttt tgttcagatt gccatatgaa cgatgttgtg
    10441 ccggaatacc gtgcctctgc tcactattca aaccgcagcg gtgtgaaagc gatttgttca
    10501 gattgccacg taccgcacga attctttcca aaatggcaac gtaaaatcat agccgcaaaa
    10561 gaagtttatg cacactttac cggtaaagta gataccaaag agaaatttga agctcatcgt
    10621 ggcgaaatgg cagaacgtga atgggcacgt atgaaagcga acagctctca agagtgccgt
    10681 aactgtcata attttgatga tatggacttc actcaacaaa aaaacattgc gcagcaaatg
    10741 cacgcattgg cacaagagca aggtaaaacc tgtattgact gccacaaagg gattgcacac
    10801 aatctcccac atatggaagc gatccagaaa aactttatcc ctgatgattt gctgaaaaaa
    10861 gcagaagaga agaaataact taacgtgttt caatagaaaa ccctcaattt ccattgaggg
    10921 tttcttttta gtagccaagt tagtaaccaa tggcattgac atttggtaaa aaatcgttgg
    10981 aatttaaccg cttgacgacc gtttctatcg tgccgttttc gtgcaattca atttcacgcc
    11041 agccttgtgg ttgtaagtct aaggtaaact ggtcgcaatt cggcttaaat tgaatgcaag
    11101 tagatggggt ggcataaatc ggaataccat gccaaatatc atcaacctgc tgatgaatat
    11161 gaccatgcac aatcgctttt acattcttaa attttgcgaa aatctcggat aattcattgc
    11221 tgttttttaa gctatgctga tccagccacg ctgagtttgt cggcaaaata ttgtggtgca
    11281 gagcaacacc gttgcggata ttgtgccaat ttttcttcta accatttaag ctgtgagtaa
    11341 gagagttcgc cataaggcat acctaccacc tgggtattta gcatcagaag ctgccattgc
    11401 tcaccgagta aaatttgttt atggggaagg atatgtttat attgcgccaa aatttctgac
    11461 atactcggct gaacatcgtg attcccctct agccacacta tcggcgagtt tagcgaatta
    11521 gttatctgtg caaaatagtg ataaccagca atattgttat cttgaatcaa atcacctgtg
    11581 gcaagaacta gatcaaaata ttgcttcgct tgtttgttta tttgctcaat aactgccgaa
    11641 aaacttttta ctgttttgac attaagtaga gtttcctccg gattggagaa aaggtgaggg
    11701 tcagttattt gtaataactt aattacccct gttacgggta acttaatata ttcgtgcatt
    11761 cgttttgcat ccattcaaat tctagtaatt tttaactggt gagattctat cagtaagcta
    11821 aaagtaaaaa tgtggactat gccgcatttt tgaaaaaaag tgagattaat caaaaaaaat
    11881 ttattatttt cgatagctaa gacaatggta agtttgatta atactttcat attgtaatag
    11941 atgattttta tactgtttgg ctgtttctag aacaacttct ttatattttt gattgatatc
    12001 aaggcaaatt gtttctgaag ataaaataat taacacttat tagttaataa aaaaggagtt
    12061 gaaatgcgtt cagaactatt aaaaacgctt ttgctatcag ggctgatttt accgctattg
    12121 atggcgtttg ccgtaggagc ttacggcttt attgtgtgga tgttacaact gtttgttatt
    12181 ggtttaccgg gagcgtagaa aatgttaggt tcaatcaaag cattatttac tcggcctgca
    12241 accaaaatcg ggttgggagt gttagttggt atcggtttta ttgccggagc aattagctgg
    12301 catcaattta ataaagcgat ggatcatacc agcactgaag agttttgcgt gagttgccac
    12361 tcaatgcagc agccgttaga ggagcttaaa aaaaccgccc actggtcgaa taaaagcggg
    12421 gtaacggcaa cctgttcaag ctgccactta ccgcacgatt ataccgaaaa atatgcccgc
    12481 aaagtgcagg caagccgtga ggcgtgggca gaattatccg gcaaatataa agaagaaggc
    12541 tcatttgaaa aacaccgctt agaaatggca gagcgtgagt gggcaagatt tgccgccaat
    12601 ggttcaaaag agtgtaaggc ttgccacagc tatgagcgaa tgaattttga taaaatgggc
    12661 gaagcagctc gtaaggcaat gaaaccggct gcggagaaag atcaaagttg tatggattgc
    12721 cacaaaggca ttgctcacca cttaccgaaa gccgatgaaa atgcactgaa atcgaagttt
    12781 gatcagtttg tgaataaatc cccaaaccaa ggcaaatctt actacaccaa agggaatgcc
    12841 gaattatttc tagacgaagg tttaagccaa gctatcgggc aattagaaac cgccgtgccg
    12901 gtggcatttg tgcaagcggg caaaaatgcg gatttggttg agctgacaat gtggcgtaaa
    12961 gagaaaggtt tcggtcgcat ttggtatcac gatttcggga aaaacattac cgatgcggtt
    13021 ctaaccaaag agtttatggc aaatcagccg aaatttgagg tgttggaaac caaagaagat
    13081 ccgattaccg gcttgaattg gcaaaaagtg aaactacaag cgtggattgg caaagctcaa
    13141 ttattagacg atgttacccc aatctgggcg aatgcagaaa accaatacaa aacccaatgt
    13201 agcacttgcc acagacagcc tgatgtcgcc cacttcgatt ccaacagctg gatcggctta
    13261 ttcaacggaa tggtcggctt taccaatatg gataaacaga ccggcaaaga ggtgctacgt
    13321 tatttacaaa tgcacgcctc cgatagtgaa gaagcgaagc attaataata ttactcccct
    13381 ctttactaaa gaggggaatt aatcgagcgt tgaataacat tctctgtact agctcacgtt
    13441 tttacaaata aagaaggaca agatatgcaa caatctcgcc gtcaattttt aaaaaatatg
    13501 tctgtgatgg ctgccgctgt cacaatgccg aatttcctcg tgcctcgcaa tgtgtttgcg
    13561 agtgaagacc tcagtcaatg gaaagtttcc ggctctcact ggggggcaat gcgtgccaaa
    13621 attgagaacg gacgagtggc agaaatcaaa ccgttcgagt ttgataaaca cccgaccgaa
    13681 atgctcaacg gcattaaagg cttaatttac agcgattccc gtatccgcta cccgatggtg
    13741 cgtttagatt ggctcaaaaa ccgccataac agcaacaccg cacagcgtgg cgataaccgc
    13801 tttgtgcggg taacgtggga cgaagccctc gacctgttct accaagagtt agaacgggta
    13861 caaaaagagt acggcccgtg ggcgttacac accgccaatg tgggctggcg ttcaaccggt
    13921 caattccaca gttgcggtaa ccatatgatc cgagcgattg cgatgcacgg taacagtgtc
    13981 ggcacggcag gcgactattc taccggtgca gggcagacca ttctgccgta tgtgctgggt
    14041 tcaaccgaag tctattcgca gggcacgtct tgggaaatca tcttaaaaga gagcgaaaac
    14101 atcattttct gggcaagcga cccggtgaag aacctgcaag tgggttggaa ctgcgaaacc
    14161 cacgaagcct atgcctactt ggaaaaactg aaagagaaag tggcagcaaa aggcgtgaat
    14221 gtgatctgtg ttgatccggt gaaaagcaaa acccagaatt tcttaggttg cgaacagcag
    14281 tacatcaatc cacaaaccga tgtgccgttt atgttggcac ttgcccacac gctctacacc
    14341 gagaacctgt acgacaaaaa attcttggat atgtacacgg tcggctttga aaaattcctg
    14401 ccgtatctgt taggcgaaac cgaagacaaa gtggcgaaaa ccccggaatg ggcggctgaa
    14461 atttgtggta tttccgccga caaaatccgt gaatttgccc gaatgcttgc cggcaaacgc
    14521 acccaattta ttttcggctg ggcaattcaa cgccaacaac acggcgaaca accgtactgg
    14581 atgggagcgg tgctagcctc aatgttaggg caaatcggct tggaaggcgg cggcatcagc
    14641 tatgctcacc attacagctc tatcggtatt cctgaatccg gtgcggcaat gccgggggcg
    14701 ttcccgctca atattgatga ggggcaaacc ccgaaatacg acaacaaaga ttacaaaggc
    14761 tacagcgaca ccattccggt ggctcgtatg accgacagct tgctccacgc cggcgaaacc
    14821 attgattaca acggcaaaaa agtgacttac gctccgttca aaatggcgat tttcaccggc
    14881 tgtaaccaat ggcaccgcca ttccgagcgg aacaaaatga aacaggcgtt ccaaaaactg
    14941 gaaaccattg tttcgatcaa ctacagttgg acggcaacct gccgcttctc cgacatcgtg
    15001 ctgccggcct gtaccccatt tgaacgcaac gatattgacg cctacggctc atacagcaac
    15061 cgtggcgtga ttgcaatgca caagctggtt gatccactct acgactctcg ctccgacttt
    15121 gagattttcc gagatctctg ccgccgtttc ggtaaagaga aagagtacag ccgtggaatg
    15181 gacgaaatgc agtgggtaga aaaactctac aaagattgtc gccgtgaaaa cagaggtaaa
    15241 tttgaaatgc cggagtttgc tgaattttgg caaaaaggct atgtgctgtt cccagagggc
    15301 aaaccttggg ttcgccacgc cgatttccgt gaagatccgg aattgcacgc acttggcaca
    15361 ccgtccggct ttatcgaaat ttacagcaac aaaatcgcct ctttcggcta tgacgattgc
    15421 aaaggtcacc caatgtggtt tgagaaagcc gaacgttcac acggcggtaa aaattccgac
    15481 aaattcccgt actggttgca atcagttcac ccggacaaac gcttacactc tcaactttgt
    15541 gaatcgaaag aactgcgtga aacttacagc gtgcaagggc gtgaaccgct ttacattaac
    15601 ccacaagatg ccgccaaaca cggcattcaa gacggagatt tagtgcgagt gtttaacgac
    15661 cgtgggcaag cgattgtggg ggcggtgctt tccgacaact tcccgaccgg cgtagtgcgt
    15721 ttacaagaag gggcttggta ttcacccctt gacgaaagcg tgggggcgat tgacacctat
    15781 ggcgacccga acacaatgac cctcgacatc ggcacgtcca agttggcaca agcggtttcc
    15841 gccaacactt gcttggtgaa catcgagaaa tttatcggca atgccccggc tccgaatggc
    15901 tttaacggtg cgattgaggt aacggcttaa tgacgttggc aatcagtcgt gaagaacggc
    15961 aatttatcta tggctggttt aacgcaatgc tcgcccgaga actttcggac gagcagttaa
    16021 atgccttaca agcgggcgaa tttgacgatt tttttgcata cctcagcgaa ttgggttttg
    16081 ccgaacaggt tgcaaaattt caagcagaag tgaccgcttg taggcagctg gaatttcctc
    16141 gtctagaact ggcggctgac tttgcccaac tgtttttatt ggaaggcaat ctcagtgcca
    16201 ttccttacgc ctccgcctat ttaagcggcg aagcgttagc gaaaaatttg gcggaaatgg
    16261 acagactgct gggcgaattt gggctgcaag ttaatcgaga aaccaaagag ccgagcgatc
    16321 acctttgcgt ttatctcacc ctttttgacc gcttgcttgc tgaaaaaccg ttggaaacgc
    16381 aacagcaatt tctgcaaaac caactgatga gctggctccc tgcgtggttc gccaaagcca
    16441 aaaccgtcaa aactaacacc ggcgtttatc cggcgttgct aaggttgttt gaacaggtgt
    16501 tgctgaagga acaaagataa agttatttat ttgagaatga tttgtattga gcgaattgag
    16561 aaaggaagtt aaaatttcaa cttccttttt tattggctgg atttatccac attattacaa
    16621 gcggttattt ttataaaaaa atttacaaag gagacattaa tgcaaaataa taaaaccacc
    16681 tacctaaatt ggtacgattt atgcgtgcta accctgatcc tgtttggtga ggcaatttat
    16741 acctcaaccc agcaatattt catcaccacc gaaatgcaag gtaattttga agaaaattta
    16801 accttttcaa atgaacaaaa ttattgggca ttactgacgc aacttgcatt gttgctgatt
    16861 gcaatgggct atctaaaatg gcggaatttt gatttcagcc gttgggtgat tcgtccctct
    16921 tggctggctt tttttagcgg tatcgggttg tttatcttgc tctcgttagc gatggatctc
    16981 tattttgaaa cattatatgg tgtttacaac ctagtgtatc cggagccatt ttgggcagta
    17041 ttgagagaag tgaatatttc taccgtgctg tatgcgattt taaacggctt ttatgaagaa
    17101 atctttttcc tcggtatttg cttggcggtt aaacctgaat atttaaaatg ggcggtgatt
    17161 tattcactga ttattcgggt gagttttcat acctatcaag gaatggaaag tgcgttagga
    17221 ttaggaattt ggctcggttt aattatttat ttgctctact cacgctcaaa gcagaaaaac
    17281 ctatttccat tttgggtggc acacagtatt gcggatattt tggggctgag tttgttggct
    17341 tatttttagt taaaggtgct acatttaagc ggcataagtt gatagagctg atcgtaccag
    17401 tagcataaaa acattattat ttgtatggaa aggtgctttt aggttacaat ataaccgata
    17461 gttattggtt ataactaaaa attattttta atttaacgga gcaaatatga agagtatcaa
    17521 aaaattaacc ctagccactt tgattttagt gggtttaact gcttgtggta gcggtggttc
    17581 aggtagcagt tctaataatg ctaaatcgaa accacaagtt gagtt
//
LOCUS       DANLXQ010000037        17169 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.037, whole genome shotgun sequence.
ACCESSION   DANLXQ010000037 DANLXQ010000000
VERSION     DANLXQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17169
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.037"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            195..3059
                     /locus_tag="PXD94_000946"
     CDS             195..3059
                     /locus_tag="PXD94_000946"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL6285829.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQGLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
     gene            complement(3130..3723)
                     /locus_tag="PXD94_000947"
     CDS             complement(3130..3723)
                     /locus_tag="PXD94_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL6285830.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTPN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(3858..5312)
                     /gene="gnd"
                     /locus_tag="PXD94_000948"
     CDS             complement(3858..5312)
                     /gene="gnd"
                     /locus_tag="PXD94_000948"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL6285831.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            complement(5395..6084)
                     /locus_tag="PXD94_000949"
     CDS             complement(5395..6084)
                     /locus_tag="PXD94_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL6285832.1"
                     /translation="MKTLGILGGMSAESTVSYYLDINRSVNKVLGGNSSAKILISSVN
                     FEEIVQCQKQGNWQAAAEILSEQARLLEQAGAEGILLATNTMHKIADQISSHISVPFL
                     HILDCVAEAISAQGLQKVALLGTKFTMSDNFYKDGLLERGITSIVPDEETQQEIHRII
                     FEELCLGKITESAKAFYLQTIQQLSEQGAEGVILGCTEIGLLINQNDNPLPFFDTAAL
                     HSQMAVEFILG"
     gene            complement(6094..6981)
                     /locus_tag="PXD94_000950"
     CDS             complement(6094..6981)
                     /locus_tag="PXD94_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDL6285833.1"
                     /translation="MNPIQLIEPNINSKIFTIRGEQVMLDVHLAELYQVDTRTFNQAV
                     KRNEERFPDIFRFQLTENEWLSLRSQFVILNENSGRGQHRKYLPYVFTEQGIAMLSAV
                     LRSEVAVQVSIKIMQAFVQMRHLLQTNSGLVQRLESVERKQLEMSGQLEQVFQSLEGN
                     KPKMQGVFFEGETFNAYQFVTELVRGAKSSLILIDNYVDDTVLTLFTKRSHNVSVTIY
                     TKQISKQLALDLEKYNSQYAHINVKIFDKSHERFLIIDEKELYHIGASLKDLGKKWFA
                     FSKMDLSMSRILEKLAENQ"
     gene            complement(7157..7855)
                     /gene="pgl"
                     /locus_tag="PXD94_000951"
     CDS             complement(7157..7855)
                     /gene="pgl"
                     /locus_tag="PXD94_000951"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL6285834.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     YPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            complement(7995..9482)
                     /locus_tag="PXD94_000952"
     CDS             complement(7995..9482)
                     /locus_tag="PXD94_000952"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL6285835.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCKIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LAYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            complement(9557..10375)
                     /gene="cysQ"
                     /locus_tag="PXD94_000953"
     CDS             complement(9557..10375)
                     /gene="cysQ"
                     /locus_tag="PXD94_000953"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL6285836.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDWDKIFLFK"
     gene            complement(10389..10931)
                     /gene="nudE"
                     /locus_tag="PXD94_000954"
     CDS             complement(10389..10931)
                     /gene="nudE"
                     /locus_tag="PXD94_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL6285837.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            11019..11435
                     /gene="hslR"
                     /locus_tag="PXD94_000955"
     CDS             11019..11435
                     /gene="hslR"
                     /locus_tag="PXD94_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL6285838.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MAFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            11679..12572
                     /gene="hslO"
                     /locus_tag="PXD94_000956"
     CDS             11679..12572
                     /gene="hslO"
                     /locus_tag="PXD94_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL6285839.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDSPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(12646..13284)
                     /locus_tag="PXD94_000957"
     CDS             complement(12646..13284)
                     /locus_tag="PXD94_000957"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL6285840.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(13312..13698)
                     /locus_tag="PXD94_000958"
     CDS             complement(13312..13698)
                     /locus_tag="PXD94_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL6285841.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEITFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISLYV"
     gene            complement(13695..14468)
                     /gene="hisF"
                     /locus_tag="PXD94_000959"
     CDS             complement(13695..14468)
                     /gene="hisF"
                     /locus_tag="PXD94_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL6285842.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDSRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            complement(14450..15199)
                     /gene="hisA"
                     /locus_tag="PXD94_000960"
     CDS             complement(14450..15199)
                     /gene="hisA"
                     /locus_tag="PXD94_000960"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL6285843.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKEREMVKGWFKKYGAEKFVLALDVNIDAIGQKIIAMSGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPEIQFQSSGGIGSLADIKAL
                     CGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            complement(15369..16214)
                     /locus_tag="PXD94_000961"
     CDS             complement(15369..16214)
                     /locus_tag="PXD94_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL6285844.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQVGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            complement(16313..16906)
                     /gene="hisH"
                     /locus_tag="PXD94_000962"
     CDS             complement(16313..16906)
                     /gene="hisH"
                     /locus_tag="PXD94_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL6285845.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTDFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
BASE COUNT         4751 a         3978 c         3561 g         4879 t
ORIGIN      
        1 caaagctgtt taaatagccg aaagcaatta tgggaaaaac gaagaaaaat gcaagaggaa
       61 caaaaaattc ccaattaatc ttttagttga ccttaattta aataactaat tacctataaa
      121 agcatttacg gcaaagatct tttttataag attaagctag tgaaaaaatt attaaacaag
      181 agaaaacaaa accaatgaca aaacaattca caatggaaga ccgttttaac ccttccgctg
      241 tagagcaagc gctctaccaa cactgggaat cgcaaggcta ttttaagccg agtgaagata
      301 ttaatgcacc gagctactgt atcgctattc cgccgccgaa tgtgacgggg agtttgcata
      361 tggggcacgc tttccagcaa accttgatgg atacgctgat tcgttttaac cgtatggagg
      421 gcaacaatac cctttggcaa acaggaactg accacgcagg gattgccact cagatggtgg
      481 tggagcgtaa aatcgcagcg gaagaaggta aaacccgcca cgattatggg cgtgaagcct
      541 ttatcaacaa aatctgggat tggaaagcct attcgggcgg cacgatcagc cagcaaatgc
      601 gtcgcttagg caactcgatt gactgggatc gtgagcgttt tacgatggac gaaggcttat
      661 ccaatgcggt aaaagaggtg ttcgttcgct tgcacgaaga aggcttgatt taccgtggca
      721 aacgcctcgt aaactgggat ccgaaactgc atactgcgat ttctgacttg gaagtggaaa
      781 acaaagaaag caaaggctcg ctgtggcatt tccgctatcc gctggctaat ggtgcaaaaa
      841 ccgcagacgg cttggattac ttagtggtgg caacgacccg tcctgaaacg atgttgggcg
      901 atactgcggt ggcggttcac cctgaagatg agcgttatca agctctgatt ggcaaaagcg
      961 taatcctacc gcttgccaac cgtgaaatcc cgattattgc cgatgagtat gtggatcgtg
     1021 agttcggtac gggcgtggta aaaatcaccc ctgcccacga tttcaacgac tacgaagtgg
     1081 gcaaacgcca cggcttgccg atggtgaatg tgatgacctt aaatgccgat attcgtgcag
     1141 aggccgaaat tatcggcaca gacggtaaac cgctttccga ctacaccgcc ccaattcctg
     1201 ccgactatca aggcttagag cgttttgccg cgcgtaagaa aattgtggca gattttgaag
     1261 ctcttgggct gttagatcaa atcaaaccac acgacttaaa agtgccgtat ggcgaccgtg
     1321 gtggcgtgcc gattgagccg atgctgaccg accaatggta tgtgtctgtc aaacctttgg
     1381 cggaagtcgc gaccaaagcg gtggaagacg gcgaaatcca attcgtgccg aaacagtacg
     1441 aaaacctgta tttctcttgg atgcgtgata ttcaagactg gtgtatctct cgccaattat
     1501 ggtggggaca ccgcattcca gcgtggtatg acgagcaggg caatgtttat gtagcacgag
     1561 atgaagcgga agtgcgtgcg aaacacaact tacctgcgga cttaccatta aagcaagatg
     1621 aagacgtact ggatacttgg ttctcgtctg gtctatggac gttctcaacg ttaggctggc
     1681 cagaacaaac caaagaactg aaaatgttcc acccgaccga tgtgctgatt accggttttg
     1741 acatcatctt cttctgggtg gctcgtatga ttatgttcac gatgcacttc atcaaagatg
     1801 aaaacggcaa accgcaagtg ccgttcaaaa ccgtgtatgt aacaggcttg atccgtgacg
     1861 agcaagggca gaaaatgtcg aaatcgaaag ggaacgtgtt agacccgatt gatatgattg
     1921 acggcatcag tctcgacgat ttgctcgaaa aacgtacagg taatatgatg cagccacaac
     1981 tggcagaaaa aatcgctaag gcaacccgca aagaatttgc cgaagggatt gtggcacacg
     2041 gcacagacgc tctgcgtttc accctcaccg cccttgccag caacggtcga gacatcaact
     2101 gggatatgaa acgtttagaa ggctaccgca acttctgcaa caaactgtgg aatgcgagcc
     2161 gttatgtgct gaccaatgac aagttggatt taagcgaagg cgacgtggaa tttagccttg
     2221 ccgatcgttg gattgaaagc caattcaacc gcacggtgga aactttccgc accgcactca
     2281 gccaataccg tttcgactta gtggcaaatg cgatctacga atttacttgg gatcaattct
     2341 gcgactggta tttagagctg accaaaccta ttttcttcaa aggcacagat gtacaacgcc
     2401 gtggcgcaag tcgcacgctg gtgaatgtat tggaaaaact cctgcgttta atccacccag
     2461 taatgccgtt catcaccgaa gaaatctggc agaaagtgaa aggctttgta ggtattgaag
     2521 ccgataccat tatgttgcaa aaattcccac aatttgaccc gcttgccatt gatgaaaccg
     2581 ccgaaagtca aatcaacttt atcaaagaag tgattgtggc ggtgcgtaac attcgtgcgg
     2641 aaagcaacat cgcaccaagc aaaggtttag atttaatcgc tcgtaatttc tctgcggacg
     2701 aagtctctat tctcaacgct aacgaagtgt tgctcaaatc gatggcaaaa cttgatagcg
     2761 tgaaagtgct ggaaaatggt gaaaatgcac cgctatcggt agcgaaactg gtcgcaaatg
     2821 gtgagatctt gatcccaatg gcaggcttta tcaacaaaga agctgagcta gctcgcctca
     2881 ccaaagagat ggataaactt aaaggtgaag tggctcgcat tgaaggcaaa ctttcaaacg
     2941 aagcctttgt cgccaaagcc ccagagcaag tcatcgccaa agagcgtgaa aaaatgcaag
     3001 agtacctgag tggattagag aaattgcagg tgcagtatca agagattgaa gcactgtaat
     3061 taactaacaa gcggtcagga tttggaaatt ttttgctaat tcagaccgct tgcttattga
     3121 agcttttcat tataaatgtt tggttaaaat cgcctctagg cggtcatagc ctgcttgggt
     3181 gaagtgtaat ccatcgtggc ataaagttag atctaaattc ccttctttgt cagcaaaata
     3241 ttcccacgtt tttacataag tcaaatcaga tggaatattt gcctcaaaat acgcattgag
     3301 ctcttttatt gcagcattat tggtggtggt aatattatta accggtgtgg cttccaatag
     3361 aaaaaagttg gcatttggat taattttgcg aagagaatcc acaatttcgc caatccactc
     3421 taacacttgt tttggcgagt aatccggctc ttttacaatg tcattaacgc ctagaaaaat
     3481 aaatacatta tcgccaacgt gttgaattcg ctcttgtcgg gtaatcacat ccaaatattg
     3541 gcgggtgctg gtgcctgata agcccaaatt ggcaactgtt tttcctgcta aattcggcgt
     3601 gccgttttcc atatcgcccc acatatcaaa cagggaatgc ccaataaggg aaatatctgc
     3661 ctgtttttca aattctgatt tttttgtttg gtagcgatta aaaatatctt gatcggtaag
     3721 cataggttct ccttaaatat aaatgaaaaa tcgctccatt attatacaaa cttgtaaaca
     3781 ttcactccaa gatattttga agaaaaatcc aataaaaaag ccacttaata tcgaattaag
     3841 tggctcaaaa agattaatta cacatcataa gtggtcgatg cggtattacc gccacgtcct
     3901 gtccagttgg tgtggaagaa ctcaccgcga gctttgtctg tacgctcata agtatgagca
     3961 ccaaagtagt cacgttgtgc ttggagtaag tttgccggta aacgtgccga ggtgtagcca
     4021 tctaagaagg taatggctga cgccatacaa ggtacaggta aaccgatttc caccgatttt
     4081 gccactactt tacgccaatc tgccaagcag ttttccaaaa tatctttgaa gtatgggtct
     4141 gagcctaaga acactaaata tgggttggct tcatacgcat cacgaatgtt gcctaagaaa
     4201 cggctgcgga taatacaacc ttcacgccat aatagggcgg tattgccgta gttgatgtcc
     4261 caaccgaatt gctcagaagc ctcacgaatc aacataaagc cttgtgcata agagatgatt
     4321 ttggaagcaa gtaacgcacg gcgtaccgct tctacccata cttttttgtc gccttcaact
     4381 ttaccaattt ctttgttaaa caatcttgaa gccgcaacac gttgatcttt aaacgcagat
     4441 acgcaacggg cgaataccga ctcggtaatc agtgttaatg gaatgccgaa atcaagggcg
     4501 ttaataccag tccatttacc cgttcctttt tgacctgcgg tgtcgagaat tttctctacc
     4561 aatggctcgc cgtcttcgtc tttgtaggca agaatatcag tggtaatatc caccaaatag
     4621 ctgtctaatt cggtgttttt ccactcttta aagatttcgt gcatttcatc atagcttaaa
     4681 cctaaaccat cttttaagaa ttggtaggct tcacaaatta actgcatatc gccatattcg
     4741 ataccgttgt gaaccatttt cacaaaatgc cctgctccgt cacgaccaac ccagtcacaa
     4801 caaggctcac ctttgtcggt tttggctgaa attgcttgga aaatcggctt cacatattgc
     4861 cacgcagatt catcaccgcc cggcataatt gacggcccgt gacgagcacc ctcttcgccg
     4921 cccgatacac ctgagccgat aaagcgaatg cctttttccg ctaacgcttt cacacggcgg
     4981 ttggtgtccg gatagtttga gttaccgcca tcaatgatga tgtcgccttc ttctaaatgt
     5041 ggcagaagag cctcgataaa ctgatccacc acctcaccgg cacgcaccat tagcatcact
     5101 ttacgcggtt tttccaactt cgccgctaaa tcttctaacg agtaagcacc gataatgttc
     5161 gtgcctttcg ccgcaccttc taagaactca tccacttttg aagtagtgcg gttgtacgcc
     5221 accactttaa agccgttgtc attcatattc aaaattaaat tttgacccat tacggcgagt
     5281 ccaatcacgc cgatatcgcc tttttgtgac attgtttgct cctattgttt ttgattctat
     5341 gcaagcggtc gaattggtaa aattttttgc aaatttgacc gcttgtaaca tttattaacc
     5401 taaaataaac tccaccgcca tttggctgtg taatgctgct gtatcgaaaa acggtaacgg
     5461 attgtcgttt tggttaatca gcaacccgat ttcggtacaa cctaaaatca caccttctgc
     5521 accttgttca ctaagttgtt gaatagtctg aagataaaac gctttggcac tttcagtaat
     5581 cttgcctaaa cagagttcct cgaaaataat gcgatgaatt tcttgttgcg tttcctcatc
     5641 tggtactatt gatgtgatac cacgctccaa taaaccgtct ttgtaaaaat tatcactcat
     5701 agtaaatttt gtgccaagga gtgctacttt ctgcagacct tgagctgaaa tcgcctcagc
     5761 cacacagtcc aaaatatgca aaaacggcac agagatatgg ctactaattt gatcggcaat
     5821 tttgtgcatt gtgttggttg ccaataaaat gccttctgct ccggcttgtt ctagcaatct
     5881 agcttgttca gataaaattt cagcggctgc ttgccaattt ccctgctttt ggcattgtac
     5941 aatctcttca aaattgacac tcgaaattaa aatcttggca ctgctatttc cacctaatac
     6001 tttattcacc gagcgattaa tatctaaata ataagaaacc gtgctttccg cactcattcc
     6061 acctaaaatg ccgagtgttt tcattaatat accttattga ttttccgcta acttttctaa
     6121 aatgcggctc attgataaat ccattttaga aaaagcgaac catttcttac ctaaatcttt
     6181 aagtgatgca ccgatatgat aaagctcttt ttcatcaata atcaaaaatc gctcgtgaga
     6241 tttatcaaat attttcacat taatgtgagc atattggctg ttatattttt ccaaatctaa
     6301 tgcaagttgc ttagatattt gtttcgtata aatcgtaact gatacgttat gcgatcgttt
     6361 cgtaaataaa gttaaaacgg tatcatctac atagttatca attaaaatca gcgatgattt
     6421 tgcacctctc actaattccg taacaaactg ataagcgtta aaggtttcac cttcaaagaa
     6481 aaccccttgc attttaggct tattgccttc aagagattga aacacctgct ctaactgccc
     6541 agacatctcc aactgtttac gctctacact ttctaagcgt tgtaccaaac cactatttgt
     6601 ttgcaaaaga tgacgcattt gcacaaaggc ttgcataatc ttaatgctaa cttgcaccgc
     6661 aacttcacta cgcaatactg ccgaaagcat tgcaatgcct tgctcggtaa agacataagg
     6721 aagatatttc ctatgttggc cacgcccact attttcgttt aagatcacaa attgtgatct
     6781 taaagatagc cattcgtttt ctgttaattg aaaacgaaat atgtcaggaa atcgctcttc
     6841 attccgtttt actgcttggt taaaggttcg agtatctacc tgataaagct ctgccaaatg
     6901 aacatctagc attacttgct cgccacgaat agtaaaaatt ttagagttaa tgtttggttc
     6961 aattaattga attggattca tctttcttcc ttattcttca gtgaagatca cttttgtgat
     7021 ctcaaaataa gaaagactat gccaattttt ggcctatacc acgagcagat ttttctgctt
     7081 tttcgatgaa tatcacaaaa atactttgca acatttttct aaaatttaac cgcttgtaaa
     7141 ggtcactgat tgtgacctag agtaagttag ccgcctcact atccaaatac cattccgtta
     7201 ccccattttc cgcctgaatt ttggcggcag gataaggtaa attttctgcc ggagtggttt
     7261 ggatttcttt taaaatttcc gctttgcttg ctcctgtgac caagtaagtg atccgtttgg
     7321 ctttttcaat taaagatgcc gttttggaaa tgcggatctg ccctgtttct gggtatttgg
     7381 cgatcacggc aacgttctga tcattaaaat ctgtttgatg tgggaacagt gaggcggtat
     7441 gcccgtctgt tcccattcct aaaataatcc aatcaaacgc caagttcggc acacaagcgg
     7501 tcaattctgc cgaaaatctt gcaagttctt gttctacatt ctcttcacca cgaatgcggt
     7561 gaatattttc gcttggaatg tcgatgtgat cgaacagtaa tttctgcact tcgccgtagt
     7621 tactttcggg atcggttggg gcaaccattc gatcatcccc ccaccagaaa tgcaggtttt
     7681 gccattgaat ttcggtatta aacggtgctt gagctaaggt tttgaataat aatttggggg
     7741 ttgaaccacc ggaaagggaa atatgcaccg ggcggttttg atggctgtat tcaataaact
     7801 ctttagcaat ttgttcaacc gcagcttggg cggtggggaa aataacttta ttcataattt
     7861 gcctcgttag atcacccctc tttagaaaag aggggagggg gagatttgat tacgatttca
     7921 atatttcgta tgagcgtata taacttctgc caaatctccc ctagcccctc tttgctaaag
     7981 aggggaactg aaatttacac tttcttcttc attgtaccgg acggttttcg ccatactttg
     8041 ccgtgtctgg cgattaattt atctgcctcg gttggccccc aagtgccgga ttcatattca
     8101 tacacacgcc cacgattagc tttgtaggct aaaatcggtt gcacaaattt ccaacaagcg
     8161 tgtacagcat cagtacgggc aaataaggtt gcatcacctt taagtgcgtc taatagtaag
     8221 cgttcataag cggtgagtaa gcttgatgat gaacttaaat cagaatagcg gaaatccatt
     8281 gagacttctt tcgcttcaaa gcctgctccc ggttttttca agccgaaacg cattgaaatg
     8341 ccttcgtccg gttgcacacg aataatcaat ttattttccg gagcattttg gctaaatacc
     8401 gggtgtggtg tagttttaaa atgaattacg atttccgtca cacgggttgg taagcgttta
     8461 ccggttcgca catagaacgg cacacctgcc caacgccagt tatcgatttt acattttacc
     8521 gccataaagg tttcagtgtt ggaatcagcc ggcacgcctt tttcttccaa gtaagccggc
     8581 acttctttgc cgtctaccgt gccacgagca tactgaccta acaccacatt gtgtttaacg
     8641 tcttcttcag ttaacggatg caagcaatat agcacttttg cgacttcatc acgcattgaa
     8701 ttagcattaa taattgccgg tggctccatt gcaaccatgg ctaatacttg caataagtgg
     8761 ttttggaaca tatcacgcat tgcccccgag ccatcatagt aaccgccacg atcttcaacg
     8821 ccgatggatt ctgccccggt aatttccaca taatcaataa aattacggtt ccaaagcggt
     8881 tcaaataagc cgttagaaaa acgtaaaacg agtaagtttt gtaccgtttc tttacctaaa
     8941 tagtggtcga tacggtagat ttgatgctct tcaaagcatt tgtggatttg tacgtctaat
     9001 tttttggcgg tttcgatatc gtagccgaat ggtttttcaa caataatccg tttccagcca
     9061 aactcttcgg tagttaagcc gtgagcggct aaacattccg gaatcacgcc atataaactt
     9121 ggtggcgtgg agaggtaata aagcgtgtta ccggctgttt gatatttatc gtgtagctca
     9181 tcaagacgag gcagcaactt ggcataatcc actgcatcag aggtatttac agcttggtaa
     9241 taaagatgtt cgcaaaattt atctaattct tcaccgcttg ctttttcaaa tttgactaac
     9301 gcatcacgca ttttttggcg gaaagattcg tcggttaatt ctgaacgaga aacacctagg
     9361 acggaaaaat gctcgcccaa tctgccaatc ttatataagt tataaagagc aggaattaat
     9421 ttacgaaaag ttaagtcacc ggaagcacca aaaatgacga tacagctatt ttcagcattc
     9481 atttatttat cctattgcta attgttaaaa ttttgttggg tgcaagatta tcataaaaat
     9541 tgctaatcgg aactacctat ttgaataaaa atattttatc ccaatctatc gagccgtcag
     9601 ccaccatcac aaaataagga ttaatcaaac tttctcgctg attataagtc agcggcgaaa
     9661 aattaaagtc gaaaattttg ccgcccattt cacttaacag cacttctgcc acagcagtat
     9721 cccattcgcc ggtgtcacca aaacggatat aacaatcggc tttcccttct gccactaaac
     9781 cggcttttag actgcccgaa ccatatttta cacactcaat ttgtggggct gaaatggcat
     9841 ttttagcttg ttctgcatgt aaatcgctgc ctacggtaac aatcaaaagg gcattctccg
     9901 cacgtttcgg tttgacaagc ggtcgaattt ctccattttc ttgcaaaaag ctaccgctac
     9961 cttccatact aaaataggtt tttcctaata tcggagcatg aataacgccc agcaccggct
    10021 gatttctacc ctcacgtttt tgcactaacg caatcaccac tgaaaattga ccggtgcggt
    10081 taataaactg ttgtgtgccg tcaatcgggt cgataatcca atattcttgc cattgttggc
    10141 gttcggcaaa cggaatatcg caaaactctt ccgataatac cggaatatct ggtgtgagtt
    10201 gcttcaagcg ttcagttaaa aattggctga taatcagatc cgcttccgtc accggtgtgc
    10261 aatcggcttt gattttaatt gccaccgagc gggcataaag ctgctttaac tgctcaccgg
    10321 ctgctttggc aatattgata atattctcga gtaggggttg agttagcgtt tgcataatag
    10381 ctccttgcct atttttttaa gtattctcgt agcataaata gagcggacaa attccgtgct
    10441 tcggcaaaat caggatctgc caataattga tcaattttat ccaacggata acgcacaatt
    10501 tccagcggtt caggctcatc gccttccaat tttgatgggt acagatcttg tgccaaaaac
    10561 aggtgcatta agccgtacat atggcttggc gaactgtaaa gcgaccgcag aaaatctaat
    10621 tttttcgccc caaaaccaat ttcttcttgt aattcacggt tcgcactctc aatcggctct
    10681 tcacctgcat ccacaatgcc tttcggaaaa cccagctcgt aacgctccga gccgaccgca
    10741 tactctttca caaaaatcag ctcgttgttt tgaatcggaa tcaccattac cgaagaacgg
    10801 cgattcggcg taagtcgttc gtaagttcgc tcttcgccat tagaaaagcg taaatcgacc
    10861 gcttgcacct caaaaatgcg agatttcgct actgttttaa tgttcaaaat ttcaggaagt
    10921 tgttttatca ttttatctca cctttttgag ttattttagt tgaaaaatct cactttcgct
    10981 ctaatatttc gactattgtt ttgattttaa ggcagaaaat gaaaaataaa cacgaaaaag
    11041 aacaaaacga cgccgaagtt cggctagata aatggctttg ggcggctcgt ttttataaaa
    11101 cccgttcgat tgccaaagca atgattgaag ggggtaaagt ccattataat gggcaaagag
    11161 ctaaagttag caaagcggtg gagatcggtg ccatcatcaa acttcgccaa ggcaatgaag
    11221 aaaaagaggt ggaagtgtta gccctcagtg accaacgccg aggggctccg gaagcccaac
    11281 tgctctacag tgaaaccgaa aaaagcatta aacaccgtga ggcaatggca tttgcccgca
    11341 aagcgaatgc tctttcaatg ccacaccctg atcgccgtcc aaacaaaaaa gaacggcggg
    11401 atttaatcaa attcaaagag caagaattta gctaagccct tgcaatcaga aaaataatcc
    11461 ctatattgaa tatcctaagt aggggcgaaa aatttttcgc ccctatataa ttatttcggg
    11521 cgaacattga ttcgccccta cagcgttcga tttgcacatt tttagccaca aaatgcaatg
    11581 cattttgaac gttggctttg ccaacggctc cgtaggagcg aacaaaatta gaaataattt
    11641 tgtgagtaat aatcgaccgc ttgcaacgag gaatcaaaat gaattacacc aaagacaatg
    11701 acaaactcta ccgctattta ttcaaagacc gagcggtacg gggcgaatgg gtgcgtttaa
    11761 acaacacctt taccgagacc ttaaacaccc atcactaccc taaagcagta caaaatttac
    11821 tcggagaaat gttagtcgca accagtttgc tgactgcaac aatgaaattt aacggcaaca
    11881 ttactgtgca gattcaaggt gacagcccgt taaaattggc ggttgtgaac ggtaacgacc
    11941 aacagcaact ccgtgcttta gcccgtgttc aggcagatat tgcagacaca gcctcgttag
    12001 cggaaatgat cggcaatggt gtattagtga tttctattat cccaaccgat ggtgaacgtt
    12061 atcaaggggt gattgctctc gataaaccaa ccattcgtga atgtttagaa gattacttcg
    12121 cccgctccga gcaattacaa acccacctcg ttatccgcac aggcgaattt gaaggcaaag
    12181 cagtggcagg cggtatgttg ctacaaatta tgccggacgg cacaggctca agagatgatt
    12241 ttgagcattt agccacgctc actgaaaccg taaaagatga agagttattt ggtttagagg
    12301 cagaagaatt gctctatcgc ctctaccacg aagagcaagt agaagtttac ccaccacaag
    12361 atactgaatt taaatgtggc tgctcaagag agcgttcagg ctccgcatta ttactggtgc
    12421 cggcagaaga aatcgaagaa atgttggagg aaaaacaagg cgtgatcgat atgcaatgtg
    12481 aatgctgcgg gacacaatat ttctttgaca aagcggcgat tgaaggcttt aaggaagaaa
    12541 ctgaaaaatt aagtaagtta gggttaaatt aagctaaaca agcggtcaag tttcttgtct
    12601 tttttgcaat ttgtaaaaaa acggtgaaat ttgaccgctt gcattttact tatttgagcc
    12661 ttctgggtgt aacccaatcc ctttgtggcg ttcttttagt ttagcattga tctctgccca
    12721 cgagagatcg gcattgtgca ataacaccgt taagtgatac atcagatcag ccacttcacc
    12781 gaccaactct tcccgatcat ttttcattgc cgcaatcacg ctttctactg cctcttcccc
    12841 tactttttgg acgatttttg gcgtgccttt ggcgtaaagt ttagcggtat aagaactttc
    12901 cggatcagca tttttacgtt cggctatcat tcgttctaat ttgctgaaaa agatccaatc
    12961 tggttgttct tgttcaaact gatgaaaaca gctctccgcc cccgtatggc aggtttcgcc
    13021 gatagggtta gcgaggatta acagggtgtc gttatcacaa tccaaactca tatccaccac
    13081 gtttaggaaa ttgcccgagg tttcgccttt cgtccacagc cgttgtttag tacgggagta
    13141 aaaagtcact cgcttttcag ccagcgtttt ttcgagggct tgttggttca tatatcctaa
    13201 catcagtact tcgcaggttt gggcgttttg gacgataacg ggcagtaagc cgtccacttt
    13261 ttgccagtct attttatttt gcattttttc ttcctttttt tgaccgcttg tttagacata
    13321 taggcttatt tcaatcaaat taccatcagg gtcacggcaa taaaccgact caatttttcc
    13381 cactgccccc gtacgaggca caatcccacc ttccaaaatc tccacctgat gtcgctgtaa
    13441 cacatagata acctcctgaa gtggagttac cgttaagagg cataaatctg ccgtgccaca
    13501 atcggcattt tgggcattgg gtaggatttc tgcacctttt tggtggaggt tgattttctg
    13561 tgtgccaaac aaaagcgctt tgcgattatc gccaaaggtg atttcttgca tacccaaaat
    13621 ggtcgtataa aaatcaatgg ttttttgcat atcggcaacg gttaaaacca gatgatctaa
    13681 ggcagtaatc ttaatcatct acgcacctca accccttcct tcgccaaata ttccttcaac
    13741 tccccaatat tgataatttg tttatgaaac acactcgctg ccaacgcacc atccacattt
    13801 gcctcaataa aagcatcacg gaaatgcacc atttcgcctg caccgcctga ggcgatgagt
    13861 gggacttggc aaacttcacg cacttttttc agttgcacca aatcgtagcc gttgcgtacg
    13921 ccgtcttggt tcatcatatt cagcacgatt tcgcctgctc ctcgcttttg cacttcctcc
    13981 acccaatcca acaattgcca ttcggtttgg cgggtgcgtt tttcatcgcc ggtatattga
    14041 ttgacccaat atttgccggt ttctgcctcg aaccagctat caataccaac caccaccgcc
    14101 tgcacgccaa agcgctttgc cagttcggta atcagctcgg ggttggcaag ggctggggaa
    14161 ttgatggaga ttttgtccgc tccgaaagcg aaaatctgct ccgcatcggc aatggttttg
    14221 attccgcctg ccacacagaa cggaatatca atcacctgtg ccacacgttc cacccagctt
    14281 ttatccaccg ttctgctgtc acttgaggcg gtaatatcgt aaaacaccaa ctcatccgcc
    14341 ccctcttggg cataacgctg ggcgagtggc acaatgtcgc cgatgatttc gtggttgcgg
    14401 aactgcacgc ctttcacaac ttgcccgtca cgcacgtcta aacaaggaat tatccgtttt
    14461 gccaacattc gattgcctcc gctacattaa atttaccttc caacaacgca cgaccgacaa
    14521 tcacgccagc cacgcccgta ccacaaagcg ctttgatgtc ggcaagcgag ccgataccgc
    14581 ctgaggactg aaattgaatc tctgggtatt tggcacagat ttctcgatag agatctacat
    14641 ttgaacctgc cagcgtgccg tctcgggaaa tgtcggtgca taaaacgtgt tgcaagccca
    14701 cggtttgata atcttcaatc aactcctcga gcgacacacc acttgcctct tgccagccgc
    14761 tcatcgcaat gattttttga ccgattgcat caatgttcac atccagtgcc agcacaaatt
    14821 tctccgcacc gtactttttg aaccagcctt taaccatttc acgctctttt accgcagtcg
    14881 aaccaatcac cacacggttt gctccaacag caagtaaatc cgccacatcc tgctcggtgc
    14941 ggataccgcc gcccacttga attttgcagt gggtttcacg aataattttg ccgataagtg
    15001 cggtctgtct ttgagccgga tttttcgccc ctgtaagatc gaccaaatgt aactgctccg
    15061 ccccttgtgc caagtagtcg gcaaattggg caatcgggtc atcggcataa gtcgtctgtt
    15121 tcgcataatc gccctgatgt aggcgaacta cttgcccatc gataaggtcg agggcgggga
    15181 taatgatgga tttttgcata ttattgttcc tgtgtgccta gcacgagccg tgctaggctg
    15241 aataatccca gtcgcgctgc gactgcctct ctaagctacg gagtagcttg gtttatctag
    15301 ccccatacgg ctcgtatggg gtacaagcgg tcaaattctt ccaaaaactt gcaaaaaacc
    15361 gaccgcactt atttcttcac ctgtttcgcc acttgttcaa tcaccgcagc gaaatcaatc
    15421 gcattttgtt gcgtaatcac cggtttgccg gtctctgcct ccaaactttc ccgagccact
    15481 tttgcaacat ttccgccccg tttggcaacg gttttatttt cttccaagcc ctgtgggtgg
    15541 ctgatttgag cgatgtcttt ggtggcagcc tcagcaagca tattcagcac caactcggtg
    15601 gtcgtcatat tgtctcgcag actttccttt ttcagccctt tgaaatcctt atattggcga
    15661 gtagtcatac cgctccacgc ctgtgtgatt tcattggtga gaatcgcaaa ttctctgccc
    15721 acttgcacac cgtgatcttg ccaagtatcc gtcagttctt ttcgcacctg aatggcttgc
    15781 aagcgttggt taatccactc acgggaatat ccctttttca agtaagtagc caaagctcgg
    15841 tcaatggtca attcaggatc gataatctca tcaatccgct ctttgcctac ctctgccagc
    15901 cacattttca acggctcggc tttcgggctt ggcacagact gaataatacg gaaaatgcct
    15961 tgtaaatccg tggcatcggt ttcccgcatt tttccgtctg gggcttttaa tttcaactgt
    16021 acgatttttt cgtacagttc aattccgccc tcttcgtcct tgattttccg ctttaaatca
    16081 ctccaataac ggcgtggatt gctactttgc gtcagcacct cgataatgtc caccaccgag
    16141 aaaaaccact cttctttttc gctatcccac accgagcgga tttggctgtt ttcaaatagt
    16201 ttaatttcgt tcatttctca ccttctttca ctggttaaat taacagtaat tttgtcgaaa
    16261 taaagcgaga aatcaagccc tattttcaaa cttttcgatc gagatcacaa aatcagacat
    16321 tctccacaaa attcctgagc aacttcgccc cagcctcgcc cgaacgctcg gggtggaact
    16381 gtacgccgta gaagttttgg ctggcaatgg cagcggaaaa cggcacgccg tagtcgcagg
    16441 tggcgatggt gttctcattc ggtagcacac cgtagctatg cacaaaatag aaatgattgc
    16501 cttgctcaat gtcgtgaaat agcggatggt cggcttggta ttgcaccttg ttccagccca
    16561 tatgcggcaa cggcaaaccg cattcaggca agcgttcggt tttgccactc attaagttca
    16621 gtgttgccac atcgccttcg tgggaaaagt ccgtcattaa ctgcattcct aagcagatgc
    16681 caagcatcgg ctgggtggcg ttgcgaatgg tgtcgataag tcggcgatcg tgcaagatcg
    16741 ccatcgccgc cgctgctgtt cccacaccgg ggagtagcaa tttatcggca gattggattt
    16801 tgttgagatc tcgcgagatc tcagcccgaa tgccaagacg gtcaaaagca aatttgaccg
    16861 aagacaggtt ggcacagcct gtgtcgatga tggtgaggtt ggtcatttag tttcctgagt
    16921 taagtatttc ttatagtgaa gtaaaattga ctcaatatat ctgtttcctt caaaagcttt
    16981 cttatcgaat cttttgataa ttgccaaaaa taaactatta aagaaaaaat cagatttaaa
    17041 attattattt agtaaatcta tatctagttg ctcacctttt agttttacac ttaaaataga
    17101 tttataacat tccaataact ctggagttct tgtttcatgc aatataatta caggtccatt
    17161 cctagaagg
//
LOCUS       DANLXQ010000038        17138 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.038, whole genome shotgun sequence.
ACCESSION   DANLXQ010000038 DANLXQ010000000
VERSION     DANLXQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17138
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.038"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            16..1278
                     /locus_tag="PXD94_000963"
     CDS             16..1278
                     /locus_tag="PXD94_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL6285846.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGGFVFAFRVL
                     PVIIFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLV
                     VKPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAK
                     IMVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNG
                     ILGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYL
                     EFAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGI
                     KAVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            1423..2199
                     /gene="deoC"
                     /locus_tag="PXD94_000964"
     CDS             1423..2199
                     /gene="deoC"
                     /locus_tag="PXD94_000964"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL6285847.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGELKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            2220..2939
                     /gene="deoD"
                     /locus_tag="PXD94_000965"
     CDS             2220..2939
                     /gene="deoD"
                     /locus_tag="PXD94_000965"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL6285848.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(3015..3866)
                     /locus_tag="PXD94_000966"
     CDS             complement(3015..3866)
                     /locus_tag="PXD94_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL6285849.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKGR"
     gene            3981..4412
                     /locus_tag="PXD94_000967"
     CDS             3981..4412
                     /locus_tag="PXD94_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL6285850.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            4429..5109
                     /locus_tag="PXD94_000968"
     CDS             4429..5109
                     /locus_tag="PXD94_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL6285851.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            5173..6297
                     /locus_tag="PXD94_000969"
     CDS             5173..6297
                     /locus_tag="PXD94_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL6285852.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNFTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNGQPMDI"
     gene            6381..6998
                     /locus_tag="PXD94_000970"
     CDS             6381..6998
                     /locus_tag="PXD94_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL6285853.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVTMLANRSDLGAFGDYAKAQTPLYFNHIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKLDMNRF"
     gene            7141..8082
                     /gene="argC"
                     /locus_tag="PXD94_000971"
     CDS             7141..8082
                     /gene="argC"
                     /locus_tag="PXD94_000971"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL6285854.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENTRICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIADYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLQIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKDSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
     gene            8174..9028
                     /gene="argB"
                     /locus_tag="PXD94_000972"
     CDS             8174..9028
                     /gene="argB"
                     /locus_tag="PXD94_000972"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL6285855.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGLEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKVNKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            9041..10225
                     /locus_tag="PXD94_000973"
     CDS             9041..10225
                     /locus_tag="PXD94_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL6285856.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLASTGQEVFHKHFF
                     PFTEGFEYLPVNGIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
     gene            10408..10839
                     /locus_tag="PXD94_000974"
     CDS             10408..10839
                     /locus_tag="PXD94_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL6285857.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            10826..11293
                     /locus_tag="PXD94_000975"
     CDS             10826..11293
                     /locus_tag="PXD94_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL6285858.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQARREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            11296..12063
                     /locus_tag="PXD94_000976"
     CDS             11296..12063
                     /locus_tag="PXD94_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL6285859.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            12060..12866
                     /locus_tag="PXD94_000977"
     CDS             12060..12866
                     /locus_tag="PXD94_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL6285860.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            12866..13903
                     /locus_tag="PXD94_000978"
     CDS             12866..13903
                     /locus_tag="PXD94_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL6285861.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHYNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            13993..15213
                     /gene="agp"
                     /locus_tag="PXD94_000979"
                     /pseudo
     CDS             13993..15213
                     /gene="agp"
                     /locus_tag="PXD94_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628146.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            15521..>17138
                     /locus_tag="PXD94_000980"
     CDS             15521..>17138
                     /locus_tag="PXD94_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL6285862.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLGKYGLIL
                     EAKPYHGMNIVGESYRKIMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTEKIRERLAK
                     TIIHFNLVFSDIYAERFIIYLRYFYNQQPELLLPKLNFNYQQTNEYQFVIKLLEQLRE
                     QDSRFEFNPEVIRFLTYIAIASTDLYRFKDCNSDNYPLLLDKAWEIHRFIFTQFSAYL
                     NLSEIHDDTCRKDLLKMLIPIAMKMMLSISDDVDLGLFNQTELHRNPILAHTVEKLVD
                     DIEKNYGYSLSIREQYFIFDIFSGMVNRIELERKPLRLAIIALNGRLSTQQLKRNIKR
                     YFSDYILKIETKVLYELAIMEDKPYDYYLCNEYGKNMAIPYKPIYFADSSLNENEYAT
                     SLNEVFYHAYQYDEVLPSIKSIEIEAQYRLN"
BASE COUNT         5046 a         3300 c         3846 g         4946 t
ORIGIN      
        1 ataattagag gttctatggg tactcttaac agctttttag gcattattgt tttactattc
       61 attgcatttt tattttcgag caataaacgt gcaataaacg tgcgtaccgt attaggcgca
      121 ttagcattgc aggtagctat tggtgcattg gtgctttatg tgccggcagg gcgtgatgcc
      181 ttgaatgcga tggcaacagg tgtgtcaaaa gtgatttctt acggtaatga agggatttca
      241 tttttattcg gtggcttagt cagcgacaaa atgtttgaag tatttggcgg cggcggcttt
      301 gtgttcgctt tccgtgtact tccggtgatt attttcttct ctgcactcat ttctttgctt
      361 tactacattg gagtgatgca atgggtaatc cgtattttag gtggtttatt acaaaaagca
      421 ttaggtacat ctaaagcgga atctatgtct gcggcagcta atattttcgt tggccaaact
      481 gaagctccat tggtagtaaa accttatatc agcaaaatga cagaatctga actctttgcg
      541 attatggcag ggggcttagc ttctattgca ggttctgtta tggctggtta cgcaggaatg
      601 ggcgtaccat taacgtactt aatcgcagcc tcctttatgg cggctccggc agggttatta
      661 ttcgccaaaa ttatggtgcc acaaaccgaa aaattccgtg atgaactaga agcggtagat
      721 ttagaaaaac cggcaaatat tcttgatgca gcagcttcag gtgcttcttc aggtatgcaa
      781 ctggcattaa acgtaggggc aatgttaatt gcattcgtgg cgttaattgc attagtgaat
      841 ggtattttag gaggtattgg cggctggttc ggttatgata ccttaaactt aggtcaaatt
      901 ttcggctggg tatttaaacc gcttgcgtgg gtaatcggtg tgccttggga agaagcagaa
      961 attgcggggc agatgatcgg cttaaaatta gcggtaaatg aattcgtggg ttatttagag
     1021 tttgctaaat acttagctcc tgagtcaacc gtacatttaa cggataaaac tgttgcgatt
     1081 attacctttg cactttgtgg ctttgcgaac ttcagctcga ttgcgatttt aatcggtggt
     1141 atcggggcaa tggctccaag ccgtcgtggt gatattgccc gacttgggat taaagccgtc
     1201 attgctggct cacttgccaa cttaatgagt gcaaccattg ccggcttgtt tatcggcttg
     1261 ggtggagcag ttctctaatc cagtttgtat aaaaccacta gaaatagtgg ttttatcttt
     1321 ttttatctga cgtaaacggt tgcttttggt ttgtctattt acaagcggtc agatttttat
     1381 aaaattttac caaccaccac gcatttaata aaaggagaca ctatgaattt aaaaaaatcc
     1441 gctgagattg ctctttcgtt gatggatctc accacattaa atgacaatga tactgatgag
     1501 aaagtcatcg cattatgcca acaagcgaac accaagtttg gcacaccggc agcaatttgc
     1561 atttatccac gttttatccc gattgcccgc aaagcattga aacagcaagg tatcgagcaa
     1621 gtaaaaattg cgacagtcac gaatttccct cacggtaatg atgatattga aattgcggta
     1681 gctgagacca aagccgcagt tgcttatggg gcagatgagg tagatgtggt attcccttat
     1741 aaagcattaa ttgcaggtaa tgagcaaata ggtttcgact tggtaaaaca atgcaaagca
     1801 gtttgtgctg ccaataatgt gttgcttaaa gtgattattg aaaccggcga gttaaaatca
     1861 gcagagctta ttcgcaaagc aagcgaaatt tcgattcaag caggggctga ttttattaaa
     1921 acctcaaccg gcaaggtggc agtgaatgct acccttgaat ctgcccgcat tatgcttgaa
     1981 accattcgtg atttaggtgt ggcagaccgt gttggcttta aagctgcagg tggggtaaaa
     2041 acctcggaag aagccgccca atatcttgca cttgcggaat ctattttagg caatgactgg
     2101 gtgagtgcat cacattttcg cttcggtgca tcaagtttat tagctaattt attagcaaca
     2161 ttgaatgatg aacagcagca agctgttcaa ggttattaat taataaagag gaacataaaa
     2221 tggctactcc acacattaac gcacctgaag gtgctttcgc tgacgttgta attatgccgg
     2281 gcgatccgct tcgtgctaaa tatatcgctg aaaccttttt agaagatgca aaagaagtga
     2341 ccaatgttcg caatatgtta ggctacaccg gcacttataa aggtcgccgc atttcagtaa
     2401 tggggcacgg tatggggatt ccatcttgct cgatttacgc taaagaatta attactgaat
     2461 atggcgtgaa gaaaattatc cgtgtcggct cgtgcggtgc ggtacgtaat gatgtcaaag
     2521 tgcgtgatat tgtcatcggt gttggggctt gtaccgattc taaagtaaac cgtattcgct
     2581 tccgtgataa cgattttgcg gcgattgctg attttgatat gacattagct gctcaccaag
     2641 cagctaaagc gaaaggcatt gaagctcgtt tcggtaattt attctcagcc gatttattct
     2701 actcacctga tgtcgaaatg tttgatgtaa tggaaaaata cggcatttta ggcgtagaaa
     2761 tggaagcagc aggcatttat gcggtagcgg cagagtatgg agcaaaagca ttagcaattt
     2821 gcaccgtttc agaccatatc cgcaccggtg agcaaactac ctctgaagaa cgtcaattaa
     2881 cctttaatgg tatgattgaa attgcgttag agtcagtatt aattggcgat aagcagtaat
     2941 ttctaacctg acaagcggtg aaattttgca aaaaatttgc agaatttgac cgcttgttat
     3001 ttttgattct tcctttatct tcctttcggg ctttcgccaa actgcctttt atattctcga
     3061 ctaaactggc ttgggctttc atagccgact tgataggcgg tttcactcac gcttactcct
     3121 gcttgtagtt tttcccttgc cgcatttagt cttaacgatt tctgatattg cagtggtgtc
     3181 attgaagtta cgcttttaaa atgagcgtaa aaccccgcca cgctcattcc tacctcttca
     3241 gccagtgctg acatatttag tgtatcggca aattttcttt ctaagatttt tgttgcattg
     3301 gcaattttag caatattgcc atcttgagag agtagagagc gtatatagcc accttgctca
     3361 gattgtaata aatagaaata gagttcgttt aatatcaatg gtgcaagaaa atgaatatga
     3421 tttggagaat taagcaaatt tagtaagcgt aacaaacagt ctttaatttc attctccagc
     3481 tcccatttag tgcgttgatt aatgttttca cttattttct gtgggggaag ctgcgtgata
     3541 attttggcaa taagatcaaa atccaagcgt agtgaaatcg caagataagg ctgattttct
     3601 gcaactgata aggtttgggt aacaatcggc aaattcacag ggcaaaacat catttcctga
     3661 ttaccgaaga cttggcaatg tccattggta cagatttgcc gctcgcctaa taatactaca
     3721 cataaacttg ggtcttggat ataaccgtta aatctaatag aacgatcggc agcgtgaacg
     3781 gttagccctg aaatgggagt agcccaaagt tcatttttcg gagcgagggc aaaaatagat
     3841 tcaataatag ctcgatgagt ttgcataata atctcacttt tagaaaatta ggcatatttt
     3901 atagaaaatc aggcaagacg acaaatagca aaaagcggat aatatcccca atcaaataaa
     3961 gcatttaata ggagcaaaat atgaaaattt tttatcacac atcagcaact gcaaccggtg
     4021 gtcgtgacgg cagaacccaa gtggatgacg gttctatcgg ttttgattta gtcggtttcc
     4081 aaaatgaaag tggcaaagta ggcactaatc cggaacaatt attcgcaatg ggctatgctg
     4141 cctgctttga cagtgcaatg aatcacgttg ccccaacctt aggcattaag ccaaccaaat
     4201 ccagtaccac tgttgcagtt gggattggtc aaaaagctga tggtgctttc ggcttagatt
     4261 tagatattac cattactgtg gaaggcatta gtgcggaaga agcgaaaact ttaattagca
     4321 aggcacacga agtttgccca tattcaaatg caacccgtgg taatgtagat gtgcgtttac
     4381 acgttaaagt cgtgagtgaa tttgacttat aaaaaataaa taggggaaat gaaaatgtta
     4441 gattttgaaa ccacggtaaa aacacgacaa tcaatccgca agtttttacc gcttccgatg
     4501 acggaaactg aaatcaaaca aattcttgcc gatgcacaaa atgccccttc ggcttgtaat
     4561 acacagccgt ggttagtaca cgttgcctct ggtgaaacgc tagcacgctt aaaagctcgt
     4621 tttaaaaaga cttttgaagc gggcattaat gatgcggatt ttgagttcaa tcaaacgaaa
     4681 tttaccggtg actacgaacc acgttggcgt aaccaataca gccacgtttt taccaccagc
     4741 tatggcattc cccgtgaaga taaagccggt cgccaagtgg tgtatgatga caatattgtc
     4801 ggttatggtg cgccacatat ggcattccta tttatgccaa gcattgacgg acacgatgtc
     4861 aatattgcca gcgatgttgg tatgtattcg caaactttct tgctctcact caccgctcgt
     4921 ggttttggcg gcattccgca gcttgccctt gcgatgtttg ctaaagatgt gcgtgaagag
     4981 ttaggcattc ctgaacatta caagctcctc cacgccattt ctttcggaca cccagattgg
     5041 gacgctatgc agaacaaaca ccatttaggg cgtgtgccgg tggaagagag tgcgacctta
     5101 cattggtaat aaacaagcgg ttgatttttc gcaaaaattt gcgaaatcaa ccgtattttt
     5161 taggagaata cgatgataaa tttatttacc ccttatactc tgaataacga tgtagaaatt
     5221 aaaaaccgcc ttgtagttgc cccaatgacc cattccgcct ccaacgatga cggcacaatt
     5281 agcgagcaag aacgccattt cttgcataat cgtgcagcag atatgggctt atttatcacc
     5341 gcagcaaccg gggtaatgcg taacggcaaa gcgttttacg gtcagccgga agctcttgat
     5401 gaacgctatt tggcaagcct gacagaaact gccaatatct tgaaaaatca aggcaccaaa
     5461 gcgattttgc aaattttcca cggtggggca caagctattc ctgccttgct tgacggggcg
     5521 aatgcagttg cgccgtccga gcacgccaac ggtgctaaag ccctaacggc ggacgaagtg
     5581 aagcaaatga tccaagcttt cgctaatgcc accgatttag ctattcaagc cggttttgat
     5641 ggtgtggaaa ttcacggggc gaacggctat ttaattcaac aatttttctc tactgaattt
     5701 aatcaacgca ctgatgaatg gggcggtagc cttgaaaaac gcttgcgttt cccgttagcg
     5761 gtggttgagg cagcggtcag cgtgcgtgaa aaacaccaac gccctgattt tattatcggc
     5821 taccgtttct caccggaaga gccgggcgaa cacggcttaa ccatgaccga tacctacgcc
     5881 ctgattgatg ctttgtgcga aaagccgttg caatacttgc acatttcact ctgggatttc
     5941 ggcaaaaaag cccgccgtgg tgcagatacc aacttcaccc gtatgcaact ggtacaccaa
     6001 cacatcaacg ggcgtttacc gctgatcggc gtgggtaact tattaaccgg tgaacagatc
     6061 cgtaaagctt atgcaacagg ctgggcagaa tttatcgcct taggtaaagc ggtaatgctc
     6121 aacccgaacg tggcgacctt gttgaaagat aatcgagaca gtgagattgt cagcgaactc
     6181 gacccgcaac aagccgacca ctacggcatt cccgatagac tatggggcta ctgcgtgcaa
     6241 ggtggtgctt ggttaccgcc gcttaaaggg caaaacgggc aaccaatgga tatttaatca
     6301 ttacagacaa gcggtcattt ttttgtaaaa atttgcaatt aagtgaccgc attttttcaa
     6361 ttatctgaag gagaaaaatt atgattacgt tacattacct gaacagttcc tgctctcacc
     6421 gcatcgtttg gttgttggaa gccttaggct tggattacga gctaaaaatc taccatcgtg
     6481 aagagagcgg ttttgcacct atggaggtaa aagccttgca cccacttggc aaagcccctg
     6541 tgttgcaaga tggcgatttg gtgcttgccg aaggcaatgc gattatccag catctgattg
     6601 cccgttacgc cgaagggaaa tttgagccga ctcgtggtag cgaagcctat tcaatgtatc
     6661 actactggtt gtcgatttct gcttcattat tctcggcaaa ccttgtgaca atgttggcta
     6721 atcgctctga tctcggagct tttggcgact acgcaaaagc gcagactccg ctgtatttta
     6781 accatattga gcaaacactt tcagataaaa aatggattgt gggtgatgaa ctaaccggtg
     6841 cggatttttc tctctgtttc ccactacaat gggggcgtaa ttttgttgaa atggcaaatt
     6901 acccgaatat tgcacgctat ttagaacagg tggaaaatca ccctgcttac caaaaagcga
     6961 atcaaaaagc cctgaagctt gatatgaata gattttaata ctctttttaa tccctaacat
     7021 tttgttaggg atttttgaat ataaaatcaa ttaatgtgaa taattattca aaaataaact
     7081 tgagtttagc cttaaatagc cttagactac cgagcagaaa aagtcaaaga aggattttca
     7141 atgagcaaat acaagatttt tgttgatggg gcagtaggaa caaccgggct acgcattcac
     7201 gaacgtttaa gccaagcgga cgatattgaa ttattaacct tgcccgaaga gcagcggaaa
     7261 gatttgaacg cccgtgtggc aaaagtcgca gaggcggatt taactttcct ctgtttgccg
     7321 gatgaagcct caaaagaatt agtagcaaat gcaccggaaa atacccgaat ttgcgatact
     7381 tcaaccgctc atcgggtcaa tcctaattgg gtttatggtt ttccggaatt gagcggacaa
     7441 cgtgaaaaaa tccaaaatgc caagcgtgtt gcggtgccgg gttgccatgc cacgggctat
     7501 attgcgttgg ttcgtccact ggtggaaaaa gggattctgc ccgccgatta ccctttaagt
     7561 tgtcattcct taaccggcta ttcaggcggt ggcaaagcga tgattgctga ctatgaaaat
     7621 gaaaacagcc caaatgcttt caatgcccct cgtgtttacg gtttaactct ccaacacaaa
     7681 catttgcctg aaatgcaggc agttacccaa atgcaaaacc caccgatttt cacccctgtg
     7741 gttgctgatt attacagcgg aatgttagtc actgtgccat tgcagatctt cgccttacaa
     7801 gcggtcaaat ccggcgaaga aattgcaaat ttgctcgccg attactacca aaccagccga
     7861 ctaattcacg ttcacccttt ccaatctcta cccgaagacg gaatgcttgc cgcgaatgct
     7921 tatgcaggta aagacagctt agagattttt atttacggca acagcaccca acttttactt
     7981 gctgcccgtt ttgacaattt aggcaaaggg gcatcaggtg ccgcagtgca gtgtatgaat
     8041 attatgttgg gaagagatga ggttgagggg ttagagcttt aaatataaat gtagggacag
     8101 gtcctgtgcc tgtccgataa tcatcgccca tattcggacg ggcacaggac ccgtccctac
     8161 aagagaaaga aatatgcaac aacaagaaat caatcaattt gcaaatttat tagaaaaatc
     8221 caccgcttgt ttggtgaaat ggcagggtaa aatcattgtg gtgaaatatg gcggtaacgc
     8281 aatgattaat gaggaactga aaaaatcagt gatggcagat ctgctgttac ttcatcgttt
     8341 aggcgtaaaa gtggtactgg tacacggtgg cgggctggag atttcacaag gcgtgaaatt
     8401 attgggtaaa gaaccgcaat ttatcaacgg gttgcgtgtt accgatagcg atacgattaa
     8461 cgtggttttg caaatgttgg caggcaaagt taataaaagc cttgtcgcct tattaaaagg
     8521 gaaaggcatt gggctgtgtg gtattgatgg ggcgatgttg caatgcaaaa aattagaggc
     8581 agaggtggat tacggctttg tgggcgatat tgtgaaagtc gatccaactg tgattgaaat
     8641 tgctcttaat gcgggcttga ttccggttgt ttccacggtg ggcgttgatg aaaacgggca
     8701 ggcttataac attaatgcag acaccgctgc cagcgagatt gcgattgcgt tacaagcgga
     8761 aaaattggtc tcgatgaccg atattgcagg tttattgcga gatcgctttg atgaaagcac
     8821 cttaattccg gaagtggaag taagcgaagt gcagtccctg attgagcaag gcattattgc
     8881 cggtgggatg atccccaaaa ttgcgtgctg taccgatttc atcagagctg gtggctctga
     8941 ggcaaatatt attgacgggc gaatcgacca cgccattttg gtagaaatgt tcggtggcaa
     9001 aaacggcaca cggttttata aaaaataaca ggaaagtgaa atgacaagca gtaatacgat
     9061 taagcaatta gataaggatt atatcgccca aacctatggc agatttgacc ttgcactgtc
     9121 gcacggaaaa ggctgtgaag tgtgggattt tgagggcaat aagtatcttg attttaccag
     9181 tggcattggg gtaaatagtt taggctgggc ggatgaggat tggctacaag ctgttaccac
     9241 acaagccggc aaattagctc acacctcaaa cctgtttttt accgaaccgg caagccaatt
     9301 agcccaaaaa ttagtggctg caagcggtct aaaacgggca tttttttgca attcaggggc
     9361 agaggcaaat gaaggagcaa ttaaaaccgc ccgcaaatat agtttcgaca aatacggttc
     9421 cggtcgttca acggttttaa ccctcgtgaa ctcttttcac ggacgaacca tttcaaccct
     9481 cgcatccact gggcaagagg tattccacaa gcattttttc ccctttacag agggctttga
     9541 atatttacct gtaaatggca ttgaggcact gaaagctcgc ttggaaactg agaacgtttg
     9601 cgggattttg ctggaggtgg tgcagggcga gggcggtgtt tgcccattag aggcggatta
     9661 tctgcacgcc gtgcaaagcc tgtgccaaga aaaagatatt ttatttatga ttgatgaggt
     9721 gcaaaccggc attgggcgaa cgggcacact ttttgcttat caacaatttg atttaactcc
     9781 tggtttagtc acgctcgcta aaggcttggg cggcggcttg ccgattggtg ctttcctgtt
     9841 aggcgaaaaa gtggaaatga cattaggcaa aagcgaccac ggctcaacct ttggtggcaa
     9901 tcctgtttcc tgtgctgctg caaatgtagt aatcgataaa ataaacgccg aatttctcgc
     9961 ccaagtgcaa caaaaagggc agttgttaca gcaacgctta gcgagcttac caaaagttaa
    10021 atccgtttca ggcttaggtt taatgctcgg cgtagaattt gaggaaggca ttttagccgc
    10081 tgatattgtt gcaaaatgta tcaacaaagg tgtgctgttt ttaaccgcta aaagcaagtt
    10141 aaggctactc ccaccgctga tgatctctga acaacagata gaacaaggaa tcagagtgtt
    10201 agaggatgtc ttgaaagatt cttgagttat tataaacagt aggaatatct tcttactgtt
    10261 ttttattact attatatagt agcctacttt aatctgttgt cactttatat ttcttcttat
    10321 gtgtacgata tatcgtgcaa taacttccct tgtttatctg tataacattt tatttaatag
    10381 aatgacttat aaatcaagga gtgacttatg tctaatcaaa ttgcgttatt gttgatgagc
    10441 cacggcagtt ttgcgagggc ggcgttggcg agtgccgagc tgattgtcgg gcagcaggaa
    10501 aatgtggcag ctatcggcgt agtagcagtg gataatgtag cggacattaa acggcagatg
    10561 gagcaaacca ttgctgagtt aaacaccgac aaaggcttga ttattctcac cgatattgtt
    10621 ggtggtacgc cgatgaatct tgcctcttct caattaaccc accctaatgt ttttgcctgt
    10681 tcgggtttga atttaccttt attgctagaa gtgctgatga gccgtgacgg caatttgcaa
    10741 gaaattcggg aaaatctacc gcttgcatac caaaacggat ttagcctacg cacacaacaa
    10801 gattttgttt cacaggagga cgatgatgat ctgctttagc cgtattgatg accgtttaat
    10861 tcacggtcaa gtagtcacca cttgggttaa tttacaccgt attgaacaaa ttattgtgtt
    10921 aaatgatgcg gtggcagcgg atccaaccca aaaacagatt ttaacgatgg cagcaccgca
    10981 gggaattaaa gtggtcgcgt ttggcattga aaaatttggt gaagtgttgc agaagacaca
    11041 aattacccgc cgtactatgt tgttgtttac caatagcatt gatgtgttac gcactgtaga
    11101 actaggtgta tcgattgaca aattgaatgt cggcggaatg cgttatcaag ctaggcgtga
    11161 gcagttaagt aaagcggttt cagttacgcc agaagagcgt caagcattta aaaccttatt
    11221 ggaaaagggt gttgaagtaa cggtacagat ggtaccgaat gatgaaaaac ttaacttaca
    11281 ggagattatc tagctatgct gactgcacta cttgttgcct gctgggcagg tatttgtgcc
    11341 ttagatgata ttggcacaca gatgatccgc cgtccgttgt taatcgcacc ggtggtcgga
    11401 ttgattatgg gggatttgga aacggcgttg attattggtg ctacgcttga agtgatgtgg
    11461 atggggatcg gcaatgttgg tgcatattct gcaccggata tgatttccgg aaccgctatt
    11521 ggcaccgcac ttggtattgc tgctggtgaa gcttctactg cggttgcact cgcagtaccg
    11581 acctcactct tggcacagca gttactcatt ctttaccgct ccggcgtatg tgcattgaac
    11641 ccaattgctg aacgctgggc ggcaagcggt gattttgaca agattttccg cattcactat
    11701 atcccaatgt taattgcctt ttctattcgt gctattccaa cgttcttagc cgtctattac
    11761 ggggcggaca ttattgccca agcggtctct gctctgccgg caaaagtgat taccggatta
    11821 aatgttgcag gcaaaattat tcctgccatc ggtatcggtt tgctgatgtt aatgatgatt
    11881 aagaaagccg aactttggac gttcttaatt gctggcttta cgttggcggt ttatctcaaa
    11941 ttatcagtat tgccgattac cttaattgca ctaccgcttg cgttgattta cgacttagca
    12001 gtaagcaaaa aagctgaagc tgcaccaacc aaaaccgcag atgaagagga ttatgaccta
    12061 tgagcaataa aattaccaaa caagatttaa acaaagtgtt ttggcggatg caggtgctga
    12121 acgtcacaaa caacttccaa tcgatgcagg cgattggatt tttaagtagc tttattccgg
    12181 tattaaaacg actttataaa gatcgtccac aggaagaacg agcggaagca atgaaacgcc
    12241 acttaaaatt ttttaacagc cacgttaatt ctgatgcatt gattttagga attgccgctg
    12301 cggtagaaga gactaccaaa gaggatgaaa aagagacagt aaccgcgatt aaaaccggtt
    12361 taatggggcc gttagcaggg ctgggagaca gcttgttgaa attcacctgg ttaccgattt
    12421 gcggtagtat cggggcggca ttggcactaa gtggcaatgt gttaggcccg attttaatgt
    12481 tcttgttgta taacagtgta aacattgcga cgaagtatta cggtatccat ctaggttaca
    12541 gcaaaggggt tgatttaatt cacggtgcgg gtaataacat tttgcaacga ctcggcaata
    12601 tggcaaatgt ggttggttta atggtgattg gcgcattaat tgcctccgtt gtgaaagtca
    12661 aagtagtttc acaaattgcc gctggtgata atgtgattaa attccaagag atgttagata
    12721 aagtgatgcc gggactactc agcttacttt taactgtcgt gatctactgg attttgaaga
    12781 aaactaacgg caaatatgct gcaacgatga tccttgctac gatggggatc tgtatcggct
    12841 tagtctatgc aggaatactc ggctaatgga cagtgtgtat gacattattc ggttggaaca
    12901 gggcagatac ctgttccgcc aacagccggc tcaatcggta cagattttct ttctggacaa
    12961 ccctgacagt aaggatagca aatggctggc ggagactgat ttggcagagt ttgagctgaa
    13021 attgagcgct tgtgaagccc gtccgttctt cctcattcaa tcagcaaacg gagaacagat
    13081 tgttgctgaa cgcaccttac ctgttgccgg aatgaataat tttcgggata tggggggcta
    13141 tgtcgctcat cagggcaagc gagtgaaatg gggcaagtta taccgctccg atcatctgca
    13201 taatttaaga gacgaaggcg tggcttatct tgataaattg ggaattcaga ctgttattga
    13261 ctatcgcagc ccgaatgaag tcgccaaata cccaaatccc ccaattaatg gcagagagca
    13321 aacttttcgg cttgatccga atgcacatac tgccgagctt gctgcccaat ttagtgcaga
    13381 caagcacgat gaagatcgta atttggtcaa taaaatcatt gctcaaaaag ctgaaggtaa
    13441 tcttatcaat cgctacgaca ttgtaatggc acaatatcgt aattttgtcg aaaaagcaga
    13501 atgccaaacc gcctttgctg aaatgttaag gttggcgaca gaccctgaaa atgcaccatt
    13561 ggttcaacac tgccgtggag ggaaagatcg cactggtttc ggtgcaatgt tactgcttgg
    13621 tattttaggc gtgagtaaag ctgatattat tgctgattat atgcttactc attataaccg
    13681 ccttgctcgt aacgaagaga aaatggcgat ttaccgtacc ttcactcaag atcaagatgt
    13741 gttggactac ctattgtctt taatcgatac tcaacccgaa tttatcgaac aatcgcttaa
    13801 tactattgaa acccaatatg gtactattga gcaatatgcc caaagggtat taggtattac
    13861 tgcgaaggaa attgaagcat tacgggcaaa ttatttggcg tagaacggta gggtgtaata
    13921 ttgaacacac tacaaatgga gaaatttcat atgcgttatt ttgcaaaaca ttggtataaa
    13981 actaccgctt gtctgacaac aattttcaca gcatttcctc tttttgctca accaattgaa
    14041 aatataccaa cttcccacta cgaattagaa aaaatcatta tatttagccg acacgggtta
    14101 cgttcccctc ttacagaaaa aggcagtgat ttagaaaaat caacccctta taaatgggca
    14161 aattggagtg taccgaccgg tcatttaacc cataaaggga cgctgttgga aacctatttt
    14221 gggcagtatc ttggtcaatg gctcacacat aatgggttat tggacaaggc aagctgtgaa
    14281 agtgggaagc agattgaggt ttatgctaat agcctgcaac gtacaattgg cactgcacaa
    14341 gctttggttg ccggtgggtt ttcaggttgt aatgtacaag ttaaacacca ccagcagatt
    14401 ggaaagatgg atccggtgtt taatccaatt attcgctccg atgaccagac atttgtgcaa
    14461 acagcattaa gcaaaattga cctcaataaa acccatcagt atttagcgcc atcctatcat
    14521 ttattaagcg aaataatcga ttatgctcat tcagaaaaat gtttgcagga aaaacagtgt
    14581 gaatttttta atgatgttgg taaattaagg attaaaaagg ataaataacc aggtgtttcc
    14641 ggtacgatgc gattaagtaa aaatattgtc gatgccttat tgctgcaata ttatgaaggt
    14701 tttcctttaa aagagattgc aaataaccag cttgatagtg atcagaaatg gcaaacactc
    14761 aataaaatta aaaacgatta ccaatttatg ctacgaggag atccatttat tgctaaacat
    14821 atttcattac cgctattaaa atatattcag caagatttaa atagtgaaaa taaaataaca
    14881 cttttagtcg gacacgattc caatattata gccttacttt ctgcattaaa tgttaagcct
    14941 tataaattag cacatcaata tgaacaaact ccaataggag ggaaagtctt tattgaaatc
    15001 tggaaagaaa aaagtagcca agcacaaaaa gttaaactgg aatatgtata tcaatctact
    15061 gaccaaattc gtaaagcgat tccactaagc cttgataaac caccgcaaca tcacattttg
    15121 agtttggatt attgccaagc tgaccaatat ggattatgcg attatatgga atttaaacag
    15181 ttactggaaa aagtcattgc tgatacattg tgagattttg acaaagtgag tgaaaaaggt
    15241 agcataaaaa agtcgggaca gttgccagct gctccctgta agatgttgtt tacagcacta
    15301 gtaatgctac tatccccaaa aagctgctga aaggcagctt ttttgttgta tataaaagta
    15361 cacctttcta cgcttcattc tttttctcca attaaaccta atttaggact tctagtgata
    15421 aattcggtat catatcttaa ttcactacat atcgtgcaag ccttaaaatt gaaagggctg
    15481 acgaaatcct atttaattct gacacacata agggaatatt atgtcgcaag atattcaagc
    15541 cgatttaaaa aagatattgg cactattaag ccgtaaccgt cgatttcaat cattattttt
    15601 attactgtta agaagtgaac aatttattaa tagtgatgca ctttctacgg agttacagct
    15661 ttcagtacga acggtaaaaa aagagattaa gttattaaaa gaaaaacttg aaccttacgg
    15721 cattcagatt ttttctcgac cgttttatgg ctataaatta ttgattcagt ttgaagaatt
    15781 acacacgcaa ctaaaacagt atttccaaat atcacagccg atgacggtaa ataataaatt
    15841 tgagtctcgg gtaaatgcga ttttacgccg attactggtg agccagcgtg cattgaaagt
    15901 tgaagatttt cagagtgagc tttatttaag ttttaccagt gccttaacaa aagaatttag
    15961 tcaagttaaa gccttgttag gaaaatatgg attgatactc gaagcgaaac cctatcacgg
    16021 aatgaacatt gtaggtgaaa gttatcgtaa aataatgctc acagtacgat tatataaata
    16081 ttttacccag cttgaacatc catttggaat taaagaattt gaacagtgtt ttgcttgtcc
    16141 tatgactgaa aaaatccgtg aaaggttagc taagacaatt attcatttca atttagtatt
    16201 ttctgatatt tatgctgagc ggtttattat ttacttacgc tatttttata atcaacagcc
    16261 tgagttattg ttaccgaagt taaattttaa ttatcaacag acgaatgaat atcagtttgt
    16321 aattaagtta ctggaacagc ttcgtgaaca agacagtcgt tttgagttta atccggaagt
    16381 tattcgattt ctgacctata ttgcgatagc cagtacagat ctctaccgct ttaaagattg
    16441 taattcggat aattacccat tgttgctaga taaggcttgg gaaattcatc gttttatttt
    16501 tactcagttt tctgcatatt taaatttatc ggaaattcac gatgacactt gccgtaaaga
    16561 tttgcttaaa atgctgatcc ctatcgcaat gaaaatgatg cttagcattt cagatgatgt
    16621 ggatttaggg ctatttaacc aaacagaact acatagaaat ccaattttgg ctcatacagt
    16681 agaaaaatta gtggatgaca ttgaaaaaaa ctacgggtat agcctatcta ttcgagaaca
    16741 gtattttatt tttgatattt tttccggaat ggttaatcga attgaattag aacgtaaacc
    16801 gctccgactg gcgattattg cccttaatgg cagattaagc acccaacagt taaaacggaa
    16861 tattaagcgg tacttttccg actatatttt gaaaattgaa accaaagtgc tatatgagct
    16921 tgccataatg gaagataaac cttatgacta ctatctctgt aatgaatatg gaaaaaatat
    16981 ggctattccc tataagccta tttattttgc tgacagtagt ttaaatgaaa atgaatatgc
    17041 aacatcactc aacgaagtat tttatcacgc ttatcaatat gatgaggtgt tgcctagcat
    17101 taaatcgatt gaaattgaag cacaatatcg cctaaatg
//
LOCUS       DANLXQ010000039        16926 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.039, whole genome shotgun sequence.
ACCESSION   DANLXQ010000039 DANLXQ010000000
VERSION     DANLXQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16926
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.039"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..9544)
                     /locus_tag="PXD94_000981"
     CDS             complement(<1..9544)
                     /locus_tag="PXD94_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL6285863.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKLAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFVTLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNTATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINADRATLTTGKPQINQGNLEGFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPTVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAGQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYVLARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKSGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKYDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLAAKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAATGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVKIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRNKLTRQEIEQPIDIGHMYGWEHRRLSLAAKELNWSQKQFNDYVNARPE
                     NFRLE"
     gene            complement(9596..11371)
                     /locus_tag="PXD94_000982"
     CDS             complement(9596..11371)
                     /locus_tag="PXD94_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL6285864.1"
                     /translation="MKTPQNLILSTVLLSASFVAEANSSSLVQQARIDATQQQIDAAQ
                     QHRQQQYQQNQAEKFQPKADVRLETQINDTLTLSNHELPCYPIHQITLIDYSPDNSLT
                     TSQFQWAFEKAVKSFHLTLPHCFGGEGLAVLMKQIQNEIIGKGYVTTRVVTAEQDLRG
                     GKLVLTVIPGKIRNTLVVDSGKVPRFTKLHALTGFTFAEGDILNVRDIEQSLENLKRV
                     PTAEANIEILPSSSEKAEVGDSDLKISYSQAFPFRLNLGLDDAGSKSTGKLQTSATLS
                     IDNIFSANDLFYTSFTHSMKQKGDDNGRRASKNLTLYYSVPFGYWQLAFSHTHHRYHQ
                     EVFGAFDKSYLYAGESDTDKLTFSYLLYRDAKRKTSLSGSLWPRQSQNYIDGSEIEVQ
                     KRRMAGWEAGFSHKEYFPTATLELSANFKRGTAARGALSAPEEDYGEGTSRPKIITAS
                     ASLTKPFFWGKQPWQWQSSLNAQWNKTPLVPQDRFSIGGRYTIRGFDGELTLSGERGW
                     NWRNELSWQFNPNHAFYWAVDGGRVSGWATENQLGHHLMGTAIGFRGGVKGFSYDVFA
                     GRPMRKPEGFKTSDTVAGFNIGYNF"
     gene            11526..12611
                     /gene="queA"
                     /locus_tag="PXD94_000983"
     CDS             11526..12611
                     /gene="queA"
                     /locus_tag="PXD94_000983"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL6285865.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHRCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEEQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(12638..13176)
                     /locus_tag="PXD94_000984"
                     /pseudo
     CDS             complement(12638..13176)
                     /locus_tag="PXD94_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13189..15471)
                     /locus_tag="PXD94_000985"
     CDS             complement(13189..15471)
                     /locus_tag="PXD94_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL6285866.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSKQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWGQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSILAV
                     ASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEGISA
                     FALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAWFAL
                     SHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQTEW
                     IMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEVEAI
                     FLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLLAIY
                     PSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHGSKS
                     STGETLLSITQPAFAVISAGRWNPWKLPNKQIIERLNRRHINVLNTVETGMIRVKFYP
                     SSIEIEQARGAFSPWYKQFY"
     gene            complement(15613..16827)
                     /gene="sstT"
                     /locus_tag="PXD94_000986"
     CDS             complement(15613..16827)
                     /gene="sstT"
                     /locus_tag="PXD94_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL6285867.1"
                     /translation="MSQSLSSKILGGNLVLRIAIGLVLGVLLAFINKEWATSVGVLGQ
                     FFVKSLRAIAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFMF
                     PTTLELTASPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFR
                     HGSASSKAFLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVL
                     VGAMLFVALVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKR
                     LGVRDEIASVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICA
                     CGASGVAGGSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFT
                     AAVSYAEDQKSA"
BASE COUNT         4360 a         3986 c         3386 g         5194 t
ORIGIN      
        1 tttctaatct gaaattctcc ggtctcgcat taacataatc attaaattgt ttttgactcc
       61 aatttaattc ttttgccgct aatgataatc ggcgatgttc ccagccatac atatgaccta
      121 tatcaatagg ctgttcaatt tcttgacggg ttaatttatt gcgataatta ccatttggca
      181 atttttcata attagcataa attttctgta acgtttccga gcgtaaataa gctcggttat
      241 atagcccatc taccggatta tctgtcgata aatagcgtcc cgtttcggca tcaatttgct
      301 gagacaaacg gttaaaatgt ggatctaccc cggcttgatg gtttggcgtt tcaaaacgat
      361 tgagatcttt ttgaactact gtcaccactt tcccgtcttt cgggtctgtg aactgataag
      421 ctttatagcc atcaatattt tttacctgtt tggcatcatt cggtaaagcg tgccaacaat
      481 cattatgcac ccatacccct tcattatcgg catttgtacc tctaataaag taagtatgat
      541 ctttctctac cgtcatattg taggctttta atctttcctg tttgattttt accttagtga
      601 cagtctgcca ctcaccactt tcagcaagta agcgatagcc tttttgcaaa tgttgagcat
      661 caacccactg agcttttgct atctctccgt tgtaagaatg cccctccgaa gaaatcggca
      721 ctctgcccga taatacttgc gtaaagaacg ggtggatttt gtttgacaca atggtgtggt
      781 attttccttt gctgtctgcg acttctacat acaccgtata atcgtatgga ttactgtaat
      841 gagcttggac tttctgatag gtggttatac cggtgcgttc atttttcgcc agcaccttat
      901 cgcccacttt aatgctggaa atctgtcgat aacctttatc agttttgact tccatatcac
      961 cgtggaatga gcaagtgcct aaactagcgc tcgatctggt cttaggaatc gcaagttctt
     1021 tacctgattt ggtgactatt ttcgccgttg tccctacacc tgttttaaca agagcaccag
     1081 caccggttaa tgctgtcacc acatcagtca tcatattgcc aaactctttt ccggcattaa
     1141 aagaacctac agcccctgcc tgttcatagt aaacctgcat tttatcgagt ttagctaaat
     1201 actcctgttt agtcatatta gcaatatagg agagcgtatc ttctcttgcg atcaattctt
     1261 tcatcgccac aatagtttct attgggcttg agaacatgga gatcaatcct tcaacgctct
     1321 cagcgagatt ctcaggaaca ccagcaacgg cgcctgatac aaaactaaca tcttgcccaa
     1381 tatcaactaa tccccaatat aatcgaactt ggttttcaca gcgggcatca ccatggcagg
     1441 attttaattc ttgtgagcgt ttctgctcct gccagttcga gaggtagtta ttctccactg
     1501 cccttttccc aatatccgcc ccaaccgctg ccgattgtgt actatcccct atcaaacctg
     1561 aagccagtcc gcttgcaagc tggcttagca acgccacatt ctgtttctcg gcttcggtta
     1621 agtcttgcgt ggatttgccc ttgtagaggg ttttaactat aaagtccgct gccgcttcgc
     1681 tccctgcgcc tgcagcagcc cctgccagta cattgttccc tgtcgcagcc gcttccaccg
     1741 cacccagtaa ggcgtgggca atcaggttgg cttgggtatc cccttcggtg tactttttga
     1801 tttctgcatt taagtatggt gaggctaaac ccactgccgc ttgtcccacg tctttcgccg
     1861 ctaaaccttg caaggctgcc gtcaccgctt ccaccgctcg tttggtttta ctgcccgtgc
     1921 cgtagttttt atcaaactct ttttgcaagc ggtctaaatt ggcttctaat ttaccaatct
     1981 cttccttgct cgctttttga gcttgtgctt tgcccaattc cagcttcgct tccgtcactt
     2041 tttcacgctc actatgcgtg taaatctgat aagccttttc tgcaatctcg cctatgcctt
     2101 tcaccagctc ttgtgtttct ttcacctcac tgatatcgta tttcgtcact ttttgattgg
     2161 cgttttgcgt atctcggttg agatgattaa gcgtagtttc cgcatttagg tcttcggtga
     2221 gggtgatagc gatattttct ccaatcgctg cctttgttgt gcttcgctcg ctttcgtttt
     2281 tattgcccag taagctcagt gccgacatcg ggttcacccc acttgtccct ccgctaatcg
     2341 cgacacttcg ggttttcaat tcactgtggt tttcaatatc gccggtggtc agcgtgccgg
     2401 cttggaagcg gttgttttct ttctccgctt ggctagtcat taccgctccc tcaaaatggg
     2461 cgttcttttt cactttaacg tccattccac tcttgcctat gtcgattgca gtttggtttt
     2521 ccacctgagc agtgttcaac ttcgctccat tatatgccac atgaccattt actcctgttg
     2581 tccctaccgc caatgtgcca tgtacgctgc cttctgtgta ttcgctttta tatttgctta
     2641 tatcttgtcg acttcttgcc tcaaaatctt gtacctcacc ttcccaatga tttgctttga
     2701 cattggttgc atccagcatg agtttaccat ttgcagaatt tgtaacaatt ttacccgctt
     2761 gcagttggtt gttctgccac gtttcagttt cgatattctc cttgccttta cctccactca
     2821 ctcctgcctc aaaaccaata ccaaaactat ctctatctaa accgataaat acgcccacac
     2881 tcgcactgtg attcttattc ttagtgttgt tttgataagt atccgcaaca cctttggatt
     2941 caatacccag cttaccgtca agcagcaggg tgtctttcac tcgggtatcc accccttcca
     3001 gcgcaacttt gcccgctccc gaaatcaagg caatattgcc gccgttcagg gtgcttttgc
     3061 tgtgggtcac gctttgacta cggctttctc tttgctgttt ttgcgtgccg agactgactg
     3121 aaatcttcac cgccggattt tctgccgcag catggttttc tgtgagtgca ttgccaagat
     3181 tgcccagcgt ttctgccact ttctgagcat tttgtgctgc aatcacggca tcctccgccg
     3241 cttttaccgc atatacccct ttgagtttct cgttttgtgt tgccttcgct ttttgcaccg
     3301 attgccgtgc cgattgcacc gcatccgtta ccggtgtact caacgcaatg cttaagcccg
     3361 actggctaaa ttcataatta tcggtgctgt taataacatc tttccctgat tcaacccgta
     3421 ccccgttttt gcctgaaatc acggcggttt tttcgttgga aatcagcaca tcgacattgc
     3481 tcacgctcgc tttattgccg gcttgaatcc gaatattgcc cccttcactg cccaatgtat
     3541 tgcgtgccgt gctttggatt gccccctcta agctgttctc ttggcgtgtt ttttggctac
     3601 cgatagtaaa gccgatttca ccaccgttca agaaaccgct gcgttttgtc tcacgaatat
     3661 gctgttctga aagttggttt gttgccgaac tgagcgttac attattaccg ccgataatat
     3721 caatatcgcc actgcgtgag acggcaagaa gactttcacc ttgaatatcg tgaccggctt
     3781 tcactaaaat gtcttcaccg gttagttcag tgcctttgtg ttcggtttca gaaatattgt
     3841 cataagtcgt ttttgttttc ttcgctagcg caccaccact ttttgttttg tgatactctt
     3901 cataatcgcg agaacgtgtt gcgctaccca acaccatatc attttcagcc agtaacgcta
     3961 aacgcgcttt ggcatccaaa tccgctcctt cactgtaaag gtttttccct gaaaccacca
     4021 catcgccttt gccttgcacc cgtgaaatga ccacatcatt caaggcttca ttacgatagt
     4081 gattgccacc gcccattttt tcatcaaaac caacctgtaa agcggtcaaa ttcaactgat
     4141 tttttgcaga taagtaggtc tgcccctgtc cgtcattgat gatgtctgcg cctgctgagg
     4201 tgatgttgcc tgcctgtact tgcagcagtc cattttcatc tttaacgtgc agtagggctt
     4261 tgcggtcaag gttggtttca gcgcgtttga aaccgtctag gtcaatctgt gtggttcgag
     4321 tggtggaggt atggttaaaa ttgcccgcaa tatccagcaa aatcgcttta tccgcttcaa
     4381 tcttgccgcc gatattctct agatcaccgc tcactttggc agcaagcacg ccaccactga
     4441 gtgtgccact attctctaac cgttcggcat taaactgtgc taaacggcgg cctgctatcg
     4501 tgccttggtt tacaatctct ttactgttga tggtaagcgc atcggctgaa atcaggctgc
     4561 ctttgccgtc aatatcacct ttgcgtgcaa ggacatacac tttcggcact aataccgata
     4621 ccgtttcacc gcttggcaag cgaaccgttt gggtttcaaa ccaaacaata tcgctggtca
     4681 agcttgccac ttgctccggc gataaggtaa tgcctaaccg aagattgaat ttctgcgcaa
     4741 aggtgatgcc ggcatccatc agggctttat attggctatc aaaatccgta tagtcgccca
     4801 aaaattgtct gccggttaag cggttgattt gctctcgaat taagcgctgt tcataaaaac
     4861 catcgcccaa acgtttatgc acattgttat ggtcgtggcg tagggcattg aacatataat
     4921 cactgcttaa ccagcgtttt ttatcggtga aatccggatc ggtttcgatc agaacatggc
     4981 tatccgcttg agggttaatg cgataaagcc cttggtttgg caaacgggta tcgaccgtca
     5041 ttgagcgaac ctcaaatgcg gctgcattcg ggcttggtat gcgttcaatc gccattgttg
     5101 gcggattagg ggcatcgggt agattacccc gttcaacctg attgccttcc ggcttcacgg
     5161 catttgctgc tttagcgtcc acataatctg tataagcggt cggtgaagta ttttccagct
     5221 cgacaaaaat attcatatcc ttatggactt cttttaaacg agcgagagga ccatcatttt
     5281 catcgtagcg ggagtggcga cgtcttgtgc ctccgtggta ctcagtaaaa gagaattggc
     5341 gccttcccag ttcccaatct tggcgaacag catctgcatc atcaaggttg gcaattctac
     5401 tgcgaccaat cgggtttaac gctttgcctg caataaccca acttttatcg ttgataaatt
     5461 catcgctttg ataagtgaga tcgccacctg ccaagatctg tgccggtaag ctggctttgg
     5521 tgatattctc ttttaccata cgctctttaa tccacaaatt ccagaaatgc gggtattctt
     5581 ggcttaaatt cgctttattg tgctttgcaa tcgcttcatc taatgcttga atattggcac
     5641 catattttgc ttcccaatca attaacggtt ttaatagcac ataacgctga taagccgctt
     5701 ctgccgcttc ataggctttc agttccgctt gatatgtttg tagtcgtttt tggtattggg
     5761 cttccgtttc cgtagagcct ctaacaggtt caatcggtct tgccggaacc accggtgcaa
     5821 tgccttgccc ttgttccttc tgtaattttt cctgctctgc ctcacttaaa tgctgattcg
     5881 ctaaatcttc ttcattcatt gcttgaataa tttcaggcgt catttccggt gcatcttcag
     5941 ctggtggtgt taaattaaac cacgtccata ctgggttatc ttttttataa ttacctgcag
     6001 gtaatagagt acaatctaca ctttcatcat tcgcacagct ttgagcatag ggttttgcca
     6061 atacggtcgt ggcattcagc tggctaatat cggtaattac cggcaagttt gcttcatcat
     6121 acttttgcca atagctccac gttttgcgat agtagccttt gcggagttga ttcacattaa
     6181 ttgtcggcaa atgcccgtta tcatatacat cacttccacc tcgattatgc ttatctcttg
     6241 gcaaaataaa atgttcgttc acagtttggc gatcagtggt ctccaattcc gaacgataat
     6301 gactatttac attcgctacc ttatccgcat tcaactgaat acgcccttca gcttcaataa
     6361 tactgctgcg gttatgtagc tgccctgcgt tgccaataac ggtatcttgc tcatttaatt
     6421 gacgtccaat cgctatctct cctccgctat atattaatga accgcttttc ttattcgctt
     6481 cataaactag ggtattattt tcaattactt tagccgctaa accgagagat tgacgagcag
     6541 caatagtggc agaggtcccg tctttatctt gatttagtat cctctccgca cctaacgcca
     6601 ccttatcgcc ataaatattc cctgtgccga tattgtggat ttcttgagct ttaatcacag
     6661 tttgagcgtt atcgttgtcc gagcggctat tgattaagcc ttcgttatag acggttgaag
     6721 ttgcagtcaa acgggtatct gccgagctta ttcgggcatt ttgtgcattg gttatttggt
     6781 ttgccgtgag ctgcaagcgg tcatttgcgg ctaatttttt gcgattgaga atatcgccct
     6841 cagtctgaat aatcatcgac ctattcgcat tgatatcttt ggtaatatca aagttctctt
     6901 gcaaattcag gcgaatatcg cctgcttgta catcaccatc accggataac gctttggcag
     6961 tgatctccaa accattattt gcagaaactt ttccctcggt attcgtcatt gtgagtgatt
     7021 ttccgccgaa aatagccaga tcgccccaac ttaaaatttc cccattacgg ttatttaagc
     7081 tgtcgctgag ggtaaatcgt gctgatgagc cgatataaat accgcctttt tcattattga
     7141 gctgttttgt gctgttcagt acaaactgcg atgcaataat cccctgtatt ggctgaggtt
     7201 ggcgtgccaa ggtaccttga ttttccacct cgaaactgtt catcgtaagt gagccacctg
     7261 cttcaataag tgagccattt tccgagatcc gattatttaa gacagtcggt acattgagcg
     7321 tgatatctgt ggccgatttc agtaccccac cttggttatc caaaagcgtg ctatttaggg
     7381 tgagatttcc cacactttga ataacaccgt gccgattatc tacttgttgg gaaaccgtga
     7441 gcgctaactc ttttactgaa ctaatttctc cctgctcacg gttatctaag gattgagcgt
     7501 taatcgcaac accgctggcg gaaattcgcc cgccttgatt ttgcagttcg ctagttatat
     7561 caagggtttg ctccgtttga gaggtgatca cccccgattg attttttaac gtattgaccg
     7621 taatcgcctg tttttcgcca ctcaaaatca gaccttgctg attattcagc aatggagcac
     7681 gaatagataa ctcgcctttt gcaaccaatt tgccatcgac tgaatccaca gcatcattta
     7741 aatttagccc taaacgcttg ccactactta atagcccttg gttatttgtt aaacgattac
     7801 cgtcaatctc aaaatgtcca cccactgaaa agcgagccga gtcagtatgg atactctctg
     7861 cttttaggcg aaaatcctgc tcaccagtct gcttccaaac cgagccacga gcatttaaat
     7921 tcgcttgttg ggcctcaatc acctctgcgg ttagctggct tccttcagtg gaaagccatt
     7981 ttggggtggt gagcttgatc tgatttgcac taaactgcgc actattcgcc tgaatatcgc
     8041 ccgattttgc actgtattca acggtatggg cattattttg gctctgattg gcatcaatct
     8101 cagctgccgc cacgttaatt ttccccgaag cgatattttt tccctgcaca gtggtttttt
     8161 ctggggcagt gagttgaata gttttccctg ccacagattg agcttccagc ttccgtactt
     8221 caccttgtgc agtgtcggtt acgtccaccc cagcggcaat cagtgagttt ttgtcggcgt
     8281 gaatttggcg ggcggtgtag gtgatattgc cttttgcgac cgtttcgcca ctttgctgga
     8341 ttttagtttc ggcttttttc tgaatattgc cacccctcgc cacgatggaa ccgctatttt
     8401 gaatatcggc tttcgagtta agctgaatat tgccctgttt attttcaatt ttgccttggt
     8461 tgtcaatccc gtgttgggca ttgagttgca cttgtctttg agcgttgaac gtgccgctgt
     8521 ttacaatccg cccttgactg tcaatggtga gcgtatcggc acttgcaccg atatgcccag
     8581 cgttacgcac gcccacgccc tgttccgtac caatcaggtg gattttgccg gcatacattc
     8641 cgcccagctc acccacatca atcgcaactg tgggcttttc ttcccctgca agcggttgat
     8701 ttgtatgaat aatttgcaaa ttgttgtccg aatttgaccg cttgacggtg tttttgccgg
     8761 taatgacttt agtctctttt ttcgaccaga caccggcgtt gattttcgct tctcgggcga
     8821 ggatatcggt gtaatccact cggctgttgt ccagcccttt gccagcaacc gttaccttgc
     8881 ctttttccac tacaaaacct tctaaattgc cttgatttat ctgtggtttt ccggtggtca
     8941 gcgtggctcg gtcggcgttg ataataccgc agccgtcaca gtgcaagccg ctcgggttgg
     9001 cgataatcac ttccgctttt ttgccagcca cttccacata acctttcatt accgaaggct
     9061 tactggaatt gacttcattt aaaatcacct tcgcttcgcc acgggcaaga tacgggttgc
     9121 cttgcaccat accggcttgt tgggtggcgg tgttggtgcg gctgttgttg agaattgccc
     9181 ccttctccgc tacgtcaaat tggctgtatt tattgtggga aagcccttta tcgttcgggg
     9241 tttgaatatt gatttgtggc agcccgttgg cggtttgcaa aataatcggt tgctgatttt
     9301 tcggggcagt gctatcggct tgaataatca aagtttccgc catagcgttg tctgataagg
     9361 taacgaatcc caacgcacag aacaagctga acgcaagcgg tcgaattttg caaatttttt
     9421 gtaaaattgc accgcttgtc atactttcac tttctgattt acctgctaat tttgctaatt
     9481 cagataccac cacaatacgt tgtaaggtct tgctgaaaat agtgcgaaaa caatgcttat
     9541 tcataataat tccttaatag cacaatatac ttaatacaat atgcccaatc attcattaaa
     9601 aattgtaacc aatattaaaa ccggcaacgg tgtctgaggt tttaaaccct tccggttttc
     9661 gcatcggtct accggcaaac acgtcataac taaatccttt aactccaccc cgaaacccaa
     9721 tggctgttcc cattaaatga tgtccgagct gattttccgt tgcccaaccg gacactcgac
     9781 caccatctac cgcccaataa aaagcgtggt tcggattaaa ttgccagctc aactcattac
     9841 gccagttcca gccccgctca ccggaaagag tcagttcacc gtcaaatcca cgtatggtgt
     9901 aacgcccgcc aatcgaaaaa cggtcttgcg gaaccagagg ggttttattc cattgggcat
     9961 ttaggctact ttgccactgc catggctgct tgccccaaaa aaacggtttc gtcaggcttg
    10021 cacttgccgt gatgatttta gggcgagaag tcccttcgcc ataatcttcc tcgggagcag
    10081 ataatgcccc tcgtgctgcc gtacctcgtt tgaaattagc cgaaagctca agcgtagcgg
    10141 tcgggaaata ttctttatgc gaaaaacccg cttcccaacc tgccattcgg cgtttttgta
    10201 cttcaatttc tgagccgtca atatagtttt gcgactggcg tggccaaagt gaaccggaca
    10261 agctcgtttt acgtttggca tcacgataaa gcaaataaga aaaggttaat ttatcggtat
    10321 cactttcacc ggcgtagaga tagctcttgt caaaggcacc aaatacttct tgatgataac
    10381 gatgatgagt gtgtgaaaat gctaactgcc aatagccaaa cggcacggaa tagtaaagcg
    10441 tgagattttt actggcacgt ctgccgttat catcaccttt ttgtttcatc gaatgggtaa
    10501 aagaggtata gaacaggtca tttgccgaaa aaatattatc tatagacaga gtggcggagg
    10561 tttgtaattt gcctgtggat ttcgagccgg catcgtccag cccaagattt aagcgaaatg
    10621 gaaacgcttg cgaatagctg atttttaaat cactgtctcc cacctccgct ttttcgcttg
    10681 agctgggtaa aatctcaata tttgcctccg ccgtcggcac ccgttttaga ttttccagag
    10741 actgctcaat atctcgcaca ttcagaatat cgccttcggc aaaagtaaaa ccggttaagg
    10801 cgtgcagctt ggtaaaacgc ggtactttgc cgctatctac cactaaagta ttgcgtattt
    10861 tgcccggaat cacggttaaa actaattttc cgccacgtaa atcttgctcc gcagtaacca
    10921 ctcgtgtagt gacataacct ttgccaataa tttcattttg gatttgcttc atcaacacag
    10981 ctaacccttc accaccaaag cagtgaggta atgtcaaatg aaatgatttt actgcttttt
    11041 caaacgccca ttggaattgg ctggtagtca gagaattgtc aggagaatag tcaatgagag
    11101 tgatttgatg aatggggtaa caaggcaatt cgtgattgga aagagtaagt gtatcattga
    11161 tttgtgtctc gagccgtaca tcagctttcg gctggaattt ttctgcttga ttttgttggt
    11221 attgctgttg gcgatgttgc tgggcagcat caatttgttg ttgagttgca tcaatgcgtg
    11281 cttgttgtac aaggcttgaa gaatttgctt cagcaacaaa agaagcactt aacaataccg
    11341 tgcttaaaat cagattttgt ggggttttca taagatcaaa taagtaagac attcaacgtt
    11401 tttagtctat aaaaatttat gttgttttgc aaactccgtt ttatggagct ttatacatat
    11461 ctgacgactt tggtttttta tgctattatc tccgccattt ttatactata gagaatagaa
    11521 taaccgtgtt agtttcagat tttcatttca acttacccga tgagctgatt gctcgctacc
    11581 cgactgccga acgctctgcc agccgtttat tacacttaaa tggcgaaaca ggcgaatttg
    11641 ccgatcaaca atttgtggat ttatacgatc atatcaacga aggcgatcta ttgatcttca
    11701 ataacactcg agtgatcccg gctcgccttt acggtcgaaa acctagtggc ggcaaagtag
    11761 aggcattagt ggaacgggtg ctagatgaac accgctgctt ggctcacgtt aaagcctcaa
    11821 aagcaccgaa agaaggggct gaattgatct ttggggaaga taagctaggt gaaggcaaag
    11881 gctttacggc gacaatggta gctcgccacg atactctgtt tgaattggtg tttaacgaag
    11941 agcaaccgtt attcgaattg ctacagcaag ctggtcatat gcctcttcca ccttatatcg
    12001 accgccccga tgaagatgct gaccaagagc gttatcaaac cgtttatagc aaagtgttag
    12061 gtgcagttgc cgcccctacc gccggtttgc attttgacaa tgcaatgctt gaaaaactaa
    12121 aagcaaaagg ggtggatact gccttcgtaa cattgcacgt tggtgccggc actttccagc
    12181 cggtacgggt agaaaccatt gaagatcata aaatgcacgc cgaatatgcc gaagtgagcc
    12241 aagaagtagt cgataaaatt ctcgcaacga aagccaaagg aaaaagagtg attgcaattg
    12301 gtacgacatc agtacgttcg gttgaaagtg ccgcccaagc ggctgaaaaa gatgggaaat
    12361 tgattgctcc gttcttttct gacaccgcta ttttccttta cccgggaaaa acctttaaag
    12421 tcattgatgc gttagtcacc aatttccatt taccggaatc cacgctaatt atgttagtct
    12481 cagcatttgc cggctataga aatacgatga atgcctacgc ccacgcagta gaaactaaat
    12541 accgtttttt cagctatggc gatgcgatgt ttatcaataa aaacccaaat gcgttgagtg
    12601 atgttcctta atttatgagg gttataaggg aataacatta tgaacaaaaa caatcttcca
    12661 accattttaa gggattgttt tcaatatatt caataatttg gaagtacgat gtttcattac
    12721 gaataatgtg ttcataataa ttcctttgcc aaatttcaga aatatcggta ttttgacgaa
    12781 cccatttagt tacgccaatt ttaaaaccac gcacaataga gccgattgtt cgtgatgttc
    12841 cccgtaaggg cgaaacattt ttcgccctta tacatggggc aaatgatgat ttgcccctac
    12901 agtttcgacg atttcaataa tgccatgtaa atgattaggc ataatcacaa agctatgcaa
    12961 gcttacattt ggaaaatgat cgggtatatc ttcccaacat tgacgtacaa tattccctat
    13021 cttattaagc ctcatttcat catcaacaat ctcaccgaga aaatggtgtt tatttttaca
    13081 acaaatagta atgaaataga atccttgtga tcggtaatca taatgcttta gacgaattga
    13141 acgcctccta tgaatctccg gctgatattt ctgcattttt cttattcctt aataaaactg
    13201 cttataccaa ggggaaaatg ccccccttgc ctgctcaatt tcaatagaac ttgggtaaaa
    13261 ttttactctt atcattcccg tttctacggt atttaataca ttaatatgtc ttctgtttaa
    13321 tctctcaata atctgtttat tcggtaattt ccatgggttc catcttccag cagaaatcac
    13381 tgcaaatgct ggctgggtaa tcgataataa agtttcgcca gtgctgcttt tgctaccgtg
    13441 atgtccaacc tgtagaaaat caattttgcc gagctgatgg gcaaattggc gttcttgttc
    13501 cacgccagaa tcccctgtca gtaatatctt caagcggtcg tttttggcta aaataacgca
    13561 agagtcttgg tttttagctc gttctgcaat gtgtgacggg taaattgcca ataattgcca
    13621 ttcgccaaat tgccacgatg ttccggcaat acaaggctcg ggatttatat tgtgatacgt
    13681 caccttgctt gaactaatta accttgcttt tgggtatgct tgcaataact ccactacccc
    13741 acctgaatga tcattatcat cgtgacttag aaaaatcgcc tctacctcaa tgccatttcg
    13801 ctttaaatac ggcaagattt ccatttttgc cattgaattg cgactaccat catttgcttg
    13861 ccaactggag ccggtatcgt aaattaccgc tttattttgt tgataaatga acgccattgc
    13921 cagcccttgc ccaacatcaa acataatcca ttctgtttgg gtttgattaa agagcttagg
    13981 gagataaaaa ctcagattga acaataggga taccgctact gcacttaacc actttttata
    14041 agatagccga tataatttgc aatacagcaa tactaaaacc aataaattca gtgaaagtaa
    14101 ctgccactgt tgccaatggc ttaatgcaaa ccacgcatta gaaaatggat ctaacagcca
    14161 taggctaaat tgagcaagat agtccgccaa cagccacgtt gaaaacagat tgtcggtcaa
    14221 gagcgtaaat aaaataagcg gtacaagcaa aaagctatac aacggcacaa taatcaggtt
    14281 ggcaagcagt gcaaaagcag agatgccctc aaaaaagtag agttgaaccg gtgagaaaat
    14341 cagccaaatg ccgatttgta agtgcaaaag cgataaccac aagcggttaa attttgtgat
    14401 ttttttacga tattcatcgt ttaaaaatcg gctaagagga aaatattgat accataaaat
    14461 taaacttgct accgctaaaa ttgagagcca aaaactatca gaaagcaggc tcaatgggtc
    14521 aattaaaatc agtaacgcca ctattctccc ccaatattgc caaggggtat agtgtcgccg
    14581 cagaacttgg caaagcaaga caaacgcaat cgccagcaac gctctggcgg tcggcacggc
    14641 aaaaccggct aaatagctgt aaccaaatgc ggtgatgaag ctaactattc ttgcaaaata
    14701 aagtgaaaat ccgaccgctt gtaatgcggt aagctcgctt ttcaagcaaa gccattgcac
    14761 gcctttggca aagtaaacac caaacaacat tgccagcccg atatgcaagc ctgaaatggc
    14821 aattagatgg gcagtagcgg tttgttggaa ttgcccccag tgttctgttt tcagccacgc
    14881 cctttcacca aaagccagag ccaataaaaa gccttgcgta ggcaaagcgt ccgtttcagc
    14941 tttcacttta ttcagccaat ttgttcgcaa gctataatca gttttggcta atgtttccac
    15001 cttgcgaacc gttgctgtca tattgatatg attggcaaaa taccaacgct gtcggtcaaa
    15061 attaccgata ttgctacgag ctgaaatagg acgaagagcc agttcggctt gatagaattg
    15121 attaagtaac aacggctgtt tcgattgcca gttcaagtag atcttctcgc ctgtatttag
    15181 tgtcgcaatc gctgtttgat aatcggtttg ttttaagatt ttactgattt caaattgaac
    15241 aggcgtttta ctagcagtcg ttttttccgc attttttgca acttgaatga tctgaatata
    15301 gccaatacaa accaataagc cgagtacagc cgttaatttt gagcgtagat aaacactcaa
    15361 tatcagcact aaggttccta ttactatacc cacaaataga aacgctttag gcaaccataa
    15421 aataggcaga tgaaatagaa tcagggcaat acaaaaacgg tctaaagtca taattcggct
    15481 tactttttaa agctgagtat gccgtttgtg cttaatattt tcagaataaa ttttcagatt
    15541 ttcgacacag ttcgcaaaaa tacttgaaaa aataaaggca gactttttag cctgccttat
    15601 aaagaagaga tattatgctg atttttgatc ttcagcgtaa cttactgctg cagtgaataa
    15661 tacgtctgtt gatgaattta atgcggtttc aaccgagtct tgaatcacac caatcacaaa
    15721 acccacccca attacttgag cagcaatatc gtttggaata ttaaataaac tacaggctaa
    15781 tggaatcaat aataatgaac cgcctgccac acctgatgcg ccacaagcac agattgatgc
    15841 aactacgctt aatagtagtg ctgttgcgaa atcaggttga ataccttgtg tataagctgc
    15901 cgctagggtt aatacggtta cagtaatcgc tgcacctgcc atattaatac ttgcacctaa
    15961 tggaatagct acagaagcga tctcatcacg aacgcccaaa cgcttggcta aattcatatt
    16021 caccggaatg ttggctgctg aactgcgggt aaagaatgcc gttacaccac tctctcgtaa
    16081 acaggtaaat gtaagtgggt atgggttgcg gcgaattttc caatacacta ataatggatt
    16141 tagtactaat gccacaaata acattgcgcc aactaagact actaataaac gtgcgtaatc
    16201 ttcaaacgca tcaataccat tgatggcaac agtttcagct actaaaccaa ataccccaat
    16261 aggcgcaaat gcaatcacaa ctttcacaac aaacgaaact gcgttagcta aatcatttaa
    16321 gaatgctttt gaacttgccg aaccatggcg gaatgcaatc cctaaaccga tagaccacgc
    16381 aagaataccg ataaagtttg cattacttaa tgcgactaat ggattatcaa ctaagttaaa
    16441 aataacggtt tttagaattt caccaatgcc ttgtggcggg gttaaaccgt ctgggcttgc
    16501 agtaagctct aatgtagttg ggaacataaa gcttaaaaca acagccgtta atgccgcaaa
    16561 aaatgtacct aatacataaa gcaccagaat cggctttaat ttgctatctg aaccaacttc
    16621 tttatttgca atcgcagcca aaactaatac aaatactaaa ataggcgcaa tggcacgtag
    16681 tgatttcaca aaaaattgac ctaatacacc aacgctggta gcccattctt tattaataaa
    16741 tgccagcaat acccctagca ctaaaccaat ggcgatacgt aatactaaat taccgccaag
    16801 aatttttgat gataatgatt gactcataac ccttcacttc tgtaagacaa caaaaaataa
    16861 ggataaataa aatttatccg cctgaaaaaa taggtttata agattagcgg aaaataaaca
    16921 aatatg
//
LOCUS       DANLXQ010000040        16751 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.040, whole genome shotgun sequence.
ACCESSION   DANLXQ010000040 DANLXQ010000000
VERSION     DANLXQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16751
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.040"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..370)
                     /locus_tag="PXD94_000987"
     CDS             complement(<1..370)
                     /locus_tag="PXD94_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6285868.1"
                     /translation="MPFYAGQVKCIFIDPPYNTKSAFTHYDDNLEHSIWLSMMYPRLE
                     ILRELLAEDGSIWITLDDNESHYLKIMLDEIFGRKNFISNLIWEKKRKPSFLGKVGKV
                     TDNLFCYAKNKNELNELNDLT"
     gene            complement(438..584)
                     /locus_tag="PXD94_000988"
     CDS             complement(438..584)
                     /locus_tag="PXD94_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase N-4/N-6"
                     /protein_id="HDL6285869.1"
                     /translation="MTTLTWYGKEEAVKKAKQTPYCLLRELPDFSHNIARQPTADSRQ
                     LSFC"
     gene            complement(725..3211)
                     /gene="torA"
                     /locus_tag="PXD94_000989"
     CDS             complement(725..3211)
                     /gene="torA"
                     /locus_tag="PXD94_000989"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL6285870.1"
                     /translation="MKKQDNIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTLRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKTDNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     regulatory      complement(3230..3426)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(3430..4545)
                     /locus_tag="PXD94_000990"
     CDS             complement(3430..4545)
                     /locus_tag="PXD94_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL6285871.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     gene            complement(4687..6438)
                     /locus_tag="PXD94_000991"
     CDS             complement(4687..6438)
                     /locus_tag="PXD94_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6285872.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EDIANELCISKRTVDKHIENIFAKTHTKSRTLLAVFAITEHLICLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTASTRHEPPIFTINHHHSRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQVATAGFYVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQKVDIGIESLTLSLREQGWNVDYLELTQEQDNYAT
                     LMAQVHNLAPSIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGV
                     LWSTNSGLSENYLGRQFSQHYERFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFK
                     AASNAIRLQPHYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPE
                     LFANAQFILPPWFSEKA"
     regulatory      6602..6758
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            6747..7772
                     /gene="moaA"
                     /locus_tag="PXD94_000992"
     CDS             6747..7772
                     /gene="moaA"
                     /locus_tag="PXD94_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL6285873.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
     gene            8273..8749
                     /gene="moaC"
                     /locus_tag="PXD94_000993"
     CDS             8273..8749
                     /gene="moaC"
                     /locus_tag="PXD94_000993"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL6285874.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
     gene            8742..8987
                     /gene="moaD"
                     /locus_tag="PXD94_000994"
     CDS             8742..8987
                     /gene="moaD"
                     /locus_tag="PXD94_000994"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL6285875.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            8989..9444
                     /gene="moaE"
                     /locus_tag="PXD94_000995"
     CDS             8989..9444
                     /gene="moaE"
                     /locus_tag="PXD94_000995"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL6285876.1"
                     /translation="MFETLIEVQEAEFDQNRIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            9477..9959
                     /gene="ybeY"
                     /locus_tag="PXD94_000996"
     CDS             9477..9959
                     /gene="ybeY"
                     /locus_tag="PXD94_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL6285877.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            10037..12055
                     /locus_tag="PXD94_000997"
     CDS             10037..12055
                     /locus_tag="PXD94_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL6285878.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKRLWQKQLSKNISYSNKHLQEKIAFYHLLANAHYKTTPTDLRRLF
                     DGENQLLFSQQENEQLIGGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNL
                     PQACKLHSIRISRIAVLPEKQNQGVGKRLISQFILQNSVEKRPLDFISVSFGLTTPLH
                     AFWQSCGFQLVQITPNKEASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFT
                     EICEELQNFEQFRPLATHNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSE
                     LDIFERKGQKISKMEVENYRKLVLQNLNNKSFKIIYRFYYIFLCFCSC"
     gene            12120..13310
                     /locus_tag="PXD94_000998"
     CDS             12120..13310
                     /locus_tag="PXD94_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL6285879.1"
                     /translation="MFSHIQAAPADSILGLGEAFKADSREGKINLGIGVYMTDEGKTP
                     IVKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLG
                     GTGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWE
                     NLIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFA
                     NGLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIK
                     SIIRVLYSNPSAHGASAVAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGA
                     NKDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAI
                     VKVL"
     gene            complement(13417..14718)
                     /gene="folC"
                     /locus_tag="PXD94_000999"
     CDS             complement(13417..14718)
                     /gene="folC"
                     /locus_tag="PXD94_000999"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL6285880.1"
                     /translation="MNTLSIPKATDSLETWLSYLEQAHSKPIDMGLDRIRKVATELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     SLAVIISIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEIKNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(14728..15624)
                     /gene="accD"
                     /locus_tag="PXD94_001000"
     CDS             complement(14728..15624)
                     /gene="accD"
                     /locus_tag="PXD94_001000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDL6285881.1"
                     /translation="MSWLDRILGRDSSSSSNAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSAVEIAADLEPEDVLKFKDLKKYKDRLTAAQ
                     KQTGEKDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEKALAEKIPFI
                     CFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGVSASLAMLGD
                     VNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRKEMRDTLSRL
                     CAKLTNQPTPFKVAELVVENAE"
     gene            15933..16715
                     /gene="mazG"
                     /locus_tag="PXD94_001001"
                     /pseudo
     CDS             15933..16715
                     /gene="mazG"
                     /locus_tag="PXD94_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
BASE COUNT         4907 a         3501 c         3457 g         4886 t
ORIGIN      
        1 aagttaagtc atttaattca tttaattcat ttttgttttt tgcatagcaa aataaattat
       61 ctgtaacttt accaactttg cctaaaaaag atggtttcct tttcttttcc caaattaagt
      121 tagaaatgaa attcttccgc ccaaaaattt catcaagcat aattttcaaa tagtgacttt
      181 cgttatcatc aagagtgatc caaattgaac catcttcggc aagtaactct cgcagaattt
      241 caaggcgtgg atacatcatt gaaagccaaa tggaatgctc taaattatca tcgtagtggg
      301 taaaggccga ttttgtgtta tagggaggat caataaaaat acatttgact tgccccgcat
      361 aaaagggcaa aagagatttt aaggctgata aattatcacc ctgaataatt aaattttgct
      421 tgactttaga taaggtttca gcagaagctg agctgtcggc tgtcggctgt cggctgtcgg
      481 gcaatattat gcgagaagtc cggcaactca cgcaataggc aatatggggt ttgttttgct
      541 tttttgacgg cttcttcttt gccgtaccaa gtaagggttg tcataaaatg cacctgaaaa
      601 gtaaaagtgg attattgtaa caagcggtca aaaaaataga aagttttaca aaaaattaag
      661 caaatttaac cgcttgcaac atagaaaagg cacagtttta tactgtgcct ttggttatat
      721 gaaattagcc ttgtgtgtat ttaggctctt taaatactgt tacttctggt gcttgtccgg
      781 tgaatttttc aacctgtacc gtagcggtat ttggtgagtt accctgtgct aattttgatg
      841 tgccttcatc acgggttagc acgttagggt tgccgttttt acatagcggt tgctcgctgg
      901 tgccgagatc gagcggatcg taccaagctc cttcgtgcaa ggctaatgta cctttgataa
      961 tgccatctgt caccaccgca ccggctaata cttgaccacg tttgttaaaa atacgtacaa
     1021 tatcaccgct tgtaatgcca cgagcggcgg catcttcagt atggatcaac accggctcac
     1081 ggtcattaac cgcatattgt ttgcgtaatg atgagtaagc taactggctg tgtaaacgcc
     1141 atttagagtg tggagtcacc aatgccaacg gttcttctgc ggtggtttta cccgagtatt
     1201 cttcgtgttc catccaagtt gggtgacctt tacagtcatc ataattcatt ttggcaatca
     1261 cctcagagaa aatctcaatt ttgccggacg gtgtgcctaa tggatttagg agtgggtcat
     1321 tacgaaattc ttcataacga acccattttt tcgctttgtc tgatgcttta aaagaaagtg
     1381 gtttattctc ttgccagaat ttctcgaatt tcggcataat cacacgattt ttgcgtgctg
     1441 cctcaaatgc agtttgatag aaactttgta gccaatccat ttcagatttg ccttcggtaa
     1501 attccgcttc tttgcctgca cgtttggcta gttcggcaaa aatatcgtag tcgttttttg
     1561 cctcaaattg cggttcgacc acctgtttca tcgggaaaat attcatcatt gaataatcgc
     1621 ccgacatagt taagtcatta cgctcatagc tggtggttgc cggcagcacg atgtcagaca
     1681 tacgagccgt tggtgtccaa tggcattcat tgacgataat agtatccggt ttttgccacg
     1741 ctttcaccaa agtgttggtg tcttggtggt gggtaaacgg attaccccct gcccaataga
     1801 caattttgat atcaggataa gtaatttgtg tgccattgta atcaatggtt ttgcccgggt
     1861 tgagcagtgc atcggaaata cgagccaccg ggaatgaaga ttttgatgtt tcatctaacc
     1921 aggttttcgc tccggcttct actgccggat ttgctgtgat agagcctaaa atgccccctg
     1981 tggcactcgg cacgccgccg tttgagtaat ggtagcttaa accgaaacca ccacccggta
     2041 agccgatttg acctaacatt gcagctaggg tgactaacat ccaatgccct tgttcaccgt
     2101 gtcgttggcg ttgaatcccc cagcctgcca ttaacattgt gcgtttagat tggaaatcag
     2161 tcgctaattt tttaatggtt tcttccggca caccacaaat ttgacttgcc cattttgcat
     2221 ctttcggttg attgtctgtt ttaccgagca gatattcttc aaacttctca aaaccggtgg
     2281 tgtatttttt aaggaatgct tgatcgtgtt tgccttcagc gactaaggta tgagccatac
     2341 cgagcattaa cgcaacatca gtgccggtgt ttaccggtac ccattctgca cctaaaaatt
     2401 cgcaagattc actttttacc gggtcaatac agataatgcg tttcccgctc tctttgagtt
     2461 ttttgaagta ttcaatacct tgttgatcgg tagaagtcca cgcaatgcgt agcgtattta
     2521 acgggttagc tgaccaaagc accacaatat ctgaactttc taaaatcact tcccaactag
     2581 tttgctgttc gtacacctcg attgtaccca agacgtgtgg cataatcact tgagcagcac
     2641 cggtagaata gtcgcctttt acaccaacaa atccgccggt aacatttaaa tagcgttgta
     2701 acagtgtacg ggaagcgtgt aatgccccag aactatacca accgtaagaa ccaccaaaaa
     2761 tagcattcgc cccgtgagcc tcacgaacac gtttcatttc attcgcaact aattcaaatg
     2821 ccttatccca cgaaactcgc acccattcat cacgcccacg tagagtagta tctttattac
     2881 cttctaagta gcctttacgc accattgggt attttactcg tgtttcaccg tgtacttggt
     2941 ctgctaccac gtgttgcaat tcatttggca cagaaatctc aaaaacagaa tcagatttca
     3001 ccactttgcc attttctacc actacaccta aaggccccca gtgggcggca gttactactg
     3061 ttttcatctc ggctgctttg gcactggtgg aagctaatgc ccccactaca ccggtagtac
     3121 cactggctaa acctgcaccg gcagctaagc ttaatgatga attttttaga aaaccgcgac
     3181 gacttacgtc aatattgtct tgttttttca taataaacct cgtttgaagt taagttaaaa
     3241 atagacacct ttctaaagtc gagtggataa accatttctc gtgtatccat tggtaaaagc
     3301 accgacggta atttttgcaa aatgttagga aaaaatgacc gcttgtagta ggtgctttac
     3361 ggcttgctac catatctctg ctctcagcag acacttaggc tcacaagctc ggtatcctct
     3421 aagttgcaat taatgggaac tgcctgctac atcttttgca ttacgttgga gatagatagt
     3481 caccgcacgc acatcatcag ccgacataga tgttctgtct ttcattgaat ttaccacccc
     3541 aatccattga tttgcggtgt aatgttctgc cccaatcggt gcatggcaac cgctacagtg
     3601 tgtttggttt aagttatcgc caaaatgatt tagtgcagta atattggcag tgactgcatt
     3661 tttaggagca gtaatttcaa aattgatttc tttccaattt gagttggtga cattatcaaa
     3721 cactgttttt gtgacattca ctttgctttg agcgtcatca ggtagaagag caaccataat
     3781 acgcttgcct aaatccatat acaccacaga ttccgcacct tcttgttgcc agccattaac
     3841 cgttgcttta acctgctcgc cttctgctgt ccattgatgt aattctgcat aaggtaacaa
     3901 gcgaatttct ccaccttgag cgagctgcac tggagtcatt gcgagcgtat aaagggtttt
     3961 atcatttgtg ctaaattccg caccgtgttt agctaattcg cccattgctg cggcattatc
     4021 taccggcaca tcaggcataa aatgggcaat tcctttatgg caatcaatac aggtttgatt
     4081 agtttcttgg gctagtttgt gcattttctg tgccgcttct gtttgttcag aaagtaccat
     4141 cgcatcaaaa ctatgacagc ttttacaggt catagaatcg ttttctttca tttctgccca
     4201 aacacgttgt gccatttcta gacgatgggc ttcataggct tcttgatcag gtaatttatt
     4261 ggttacaacg gtgtgccaaa catcttttaa tgcgatgatt ttagctttga cataatgcaa
     4321 gccgtcttgt ggtagatggc aatccgaaca ctcggctcga attcccttac ggttggaaaa
     4381 atggacactg ccttcccact ctgcctgtgg gtgagtcata gaatggcagc tcacacaaaa
     4441 ttctgttgag ctggtggctt tcattgcata ttgtgtgcca cctaataccg cagcacctaa
     4501 tgccataata ccgacaaata cagcaatttt tttcatctta gccataaatt accccactta
     4561 atacactaaa aattaaagac agaggcattg tattaactcc caagaggtat aaaaataagt
     4621 aaaaatacct attacctcga aagagtgaga ataatttgat ctaaggcaaa aatatgtagt
     4681 gaaattttag gctttttcag agaaccacgg cggtaagata aattgtgcat tggcaaacaa
     4741 ctccggtgca atcacacggc tctgcccttg ttggatttgg taaaccaact gtggcaagct
     4801 gatagataag tcgtgggttt ctgcgtaagt taatccaatt tggctatcgg tattaaaata
     4861 gtaagtgccg tttaccccat aatgtggctg taagcggatc gcattggagg cggctttaaa
     4921 atgccgtggt gaaacactct gtttccacgc atttgccaga atattcgctt ggtcataagc
     4981 aatactggct tgggaataag tgggcgattt gccgaaaaac cgttcgtaat gttggctaaa
     5041 ctgtctgcct aaataatttt cgcttaaccc actattggta ctccaaagaa cgccttcgca
     5101 aagtttctcg tgtggcaaaa acgccgaagg tgcataaata ctgtaaataa tggcatttgt
     5161 tgggttgcga atgaacgttt gataaaactg tacaatatct tctgcaaaat aagaggcaaa
     5221 cacaataatg gaaggtgcga gattatgcac ctgtgccatt aatgtggcat aattatcttg
     5281 ctcttgggtg agttccagat aatccacatt ccagccttgc tcccgcaaac ttagggttaa
     5341 actttcaata ccgatatcaa ctttttgcca actcaccgtt accaccatca ccgttttatt
     5401 ctgaagccaa ggggcataat ggtaacggta atattgcaga aagtgagata tacctaagcc
     5461 ataggttaca tcactggcgc aaacctgaaa catattttca attcgttgat tcggtaaatt
     5521 ttgggcaagt ttatttgagc cactatgggt tgcaatatgc aaataaggaa tgcctttagt
     5581 tgcaattaat tcgtgtactt ccggcaagta gcaggcataa gcggtggaaa tcgcatcaac
     5641 ttcttgatca aataaatgat ggtatgcctg aagaatactt gctttatctt taacataaaa
     5701 cccagccgtt gcaacctgta cttctctgcc gtgaatacca ccttgttgat tgatggtctc
     5761 aaccgcaagt tgagtgccgt taagtagctc tcgggtatcg atttgcccaa taccctgttc
     5821 aacataaggc acgccaataa taatcggacg actatgatgg tggtttatcg taaaaatcgg
     5881 tggttcgtgt cgagttgaag ccgttacaag cggttgtttt ttcgcgataa tttgcaatgc
     5941 cagttgttca atgttaaagc tggcaagaat ggattgggtt agttttcccg gtgtgggtaa
     6001 acagattaaa tgttcggtaa tggcaaaaac agctaataag gttcggcttt tggtatgtgt
     6061 tttcgcaaaa atattttcga tatgtttatc aacagtccgt ttgctgatac aaagctcatt
     6121 ggcgatatcc tcattgctca aaccactgct aattagagtg agaagctcaa tctccctttt
     6181 actcagtcca taaggcaaat cggtatgctc tgcttgagtc aatacaaacg attggtggcg
     6241 ttctaggctg atcttaagcc attgatggtt tgaactatag taaaactgca agctattttt
     6301 atctgaatag agcaatgcct gtgcatagtg gcaaatttgc ggtaattcag caaaatagtc
     6361 tgcccctgct ttgtcttccc attctgatat ttcgccttgt tcatttacta aacaagacca
     6421 tcggctacgt ttatccattc tcacctcgaa ctaaaaacgt tagctaaaat agcaaaatta
     6481 tctcggcatt aatatgatat aaatcaaact aagcaacggt tgccagattt ttataggtag
     6541 agcatacaat ttaaattata cctcttgcct aattaggttt ctcaccataa aatgaaaacc
     6601 aatttattga agactccgag cattatttcc taactctaaa aattaaagac aaatttttga
     6661 tatggaattt atgccagtaa cagcataaag ctgttcaaag gataacttag aaatgggttg
     6721 tcctctcgtg tttagaaagg tgtcaaatgc agtctattat cccaattaaa aacgtgggtg
     6781 atgatgtcgt ttcaggttta gttgatcgct ttcagcgtca atatacttat ttgcgactat
     6841 ccattactga tgtttgtaac ttccgttgta attattgctt gcctgatggc tataagccgc
     6901 cttcacataa gcaaactttt cttagcgttg atgaaatcca gcgggtggct aaagcctttg
     6961 ccaatttagg gacggaaaaa atccgtatca ccggtggtga gccaacttta cgcaaagatt
     7021 tcctcgaaat cgctcacact atttcacaaa cacccggtat ccgtaaagtt gccctaacca
     7081 ccaacggcta tcgcctagag cgtgatgttg aactgtggca acaagccggt attaccgata
     7141 ttaatgtgag tgttgatagc cttgacccac gccaatttca actgattacc ggcgagaaca
     7201 agttgcaatc tattttaaag ggtattgata aagcctttga aattggctac ccaaaaatta
     7261 aagtcaatgc ggtgctaatg aagcaatata ccgcccctga attggataaa ttcctcactt
     7321 ggatcaaaga caagccgatt caaatgcggt ttattgagct gatggaaacc ggtgaaatgg
     7381 atagtttctt ccaagctcaa catctttctg gtcaatcggt gatgacccgc ttattacaag
     7441 aaggctggca attacaaccc aaagcgatgt ctgacggccc ggcaaaagtg ctttctcacc
     7501 ctgattttaa aggcgaaatc ggcttaatta tgccctacga gaaaaatttc tgtgcctctt
     7561 gcaaccgctt gcgtgtttcc gcacttggta aactgcatct ttgcttattt ggtgaagaag
     7621 ggattgatat tcgtgattta ctggcaagcg atgagcaaca gttacagctt gaaacccgcc
     7681 taaaatcttc cttacaaggc aagcgagaac accactattt gcatattggg gatagtgggg
     7741 ttaggaataa tttggcgagt atcggtggtt aaacaaccac cgagcgtgtt ttttctgtta
     7801 cttttctttg cttcgccaaa gaaaagtaac caaaagaaag gcgaccccag attttcctta
     7861 atccttcgct tgttgctaac ttttgggacg aacttttcaa actcgccaac tgcgttggct
     7921 caaacacgaa aagttgtcct caaaagttta cgcaatcgct acggcggaaa ataggggcaa
     7981 gcggtacatt atttaagatt tttgcaagtt tttatttaaa aataaccgct tgtaatgttc
     8041 cagcttctcc cttctgaacc gcctgagcga ctttgaattt ataggaaaaa tttagtgttt
     8101 gagccaacga agttggcgag ttttaaattt ttccgttaaa caaattcaaa taaaatgaag
     8161 atagcggtga agtagggtgt gctttctttg gttactttct ttgcacaagc aaagaaagta
     8221 acaagaagac gctcaatatt aattttacaa tcaaatttaa agagacaaac aaatgacaac
     8281 cttcacccac atcaaccaaa acggcgaagc caatatggtt gatgtttcaa tgaaacaaga
     8341 aaccgtacgc attgcccgag cggaggcatt tgtgcgaatg aatgccgaaa cattacaaat
     8401 gatcgtcagt ggtaatcacc acaaaggcga tgtattcgca accgctcgaa ttgccggtat
     8461 tcaggcggcg aagagaactt gggagctgat tccactctgc cacccgttat tgctttctaa
     8521 agtggaagtt caactggagg cgttgccgga aagcaaccaa gtgcgaattg aatcactctg
     8581 taaactaacc ggcaaaaccg gcgtggaaat ggaggcatta accgcagcta gtgttgctgc
     8641 tctgactatt tacgatatgt gtaaagccgt gcaaaaagat atgattattg aaaatgtaag
     8701 attacttagt aaaagtggcg gaaaatcagg agatttcaag gttgattaag atactttttt
     8761 tcgcccaagt gcgtgaactt gtggcaacgg atagactaga tgttgagcct acttttacca
     8821 ctgccgaagc gttgcgtgaa cacttaagcc aacagggtaa aaaatggcaa cttgcccttg
     8881 aaaaaggcaa actgctggtt gcaattaatc aaacgatctg cccgcttgaa acggagatca
     8941 aagacggcga tgaagtggca ttcttcccac cggtaaccgg gggctaaaat gtttgaaacc
     9001 ctgattgaag tacaagaagc tgaatttgac caaaaccgca tttaccaatg gctcagtgaa
     9061 caacatagcg tgggggcagc cactctattc atcggtaaag tacgtgaaat gaatttgggc
     9121 gataatgttt ccggtttgtt tcttgaacac tatccggcaa tgactaaaaa agcattgcaa
     9181 gaaattgtgg ctcaagctcg cactcgttgg gatttacaac gcgttgcggt gattcaccgc
     9241 attgggcaac tgcataccgg tgatgaaatc gtattagtcg gtgtgagttc tgctcatcga
     9301 ggtgatgcct accacgccaa cgagtttatt atggattatc tcaaaaccaa agccccattt
     9361 tggaaacgag aacaaaccca agccggtgaa cgttggattg aagggcgtga gagtgaccaa
     9421 caagccgcca aaaaatggca atagaaaaaa tggcaataat ttgcaaaagg taacgaatgg
     9481 aaaatttata tattgatttg cagatcgcct gcgaaaatac cgaaaactta ccaagtgaac
     9541 aacaatttta tacttgggta caaaaagccc tcgcagttga ggcaaaaacc gatgatttcc
     9601 ctgagagtga gatcaccatt cggattgtcg atgaagcaga aagccacgag ctaaatctga
     9661 cttatcgtgg taaagacaag ccaaccaatg tgctatcttt cccgtttgag gcccccgaag
     9721 gaatcgaaat gccactattg ggtgatttag tcatctgtcg tcaagtaatg gaaaaagagg
     9781 cggaagaaca aggtatttca gccgaatccc attgggctca tttggcgatt cacggcacac
     9841 ttcatctgct cggctacgac catattgagg aagatgaagc agtggaaatg gaaagcctcg
     9901 aaaccgaaat tatgcaaagt ttagggtatg aagatccgta tctttcagaa aaaatatagt
     9961 cttgtagggg agggtcctgt gccctccagt actccattat aatcggacag gcacaggagc
    10021 tgttcctaca agttatatga gaacgatcgc aatcataaca tctcctaaca atgccgactt
    10081 tattcaacaa ggctattttt acattcccga aaatcatcag cttaaagcta tccccttttc
    10141 caatgccaaa acgattttag gacaagaata cccttttgcc atttatacta tgcaagcaga
    10201 aaatggggtg aattttaact tagatgcttt tgcaatactt gccggcacta ttcaagaaaa
    10261 cggtacttta tttttgcttt gtcctcagtg ggataatttg gaaaatgaat tagatttcga
    10321 tgcgttacgt tggaacgaaa atcaggctat tgcctgcccg aatttctatc aacattttaa
    10381 acaattagtc gctaaatttg gttttgaagt cagaactaat ttgccaaaat taccggctac
    10441 aagcggtcaa attccttcaa aaatttacca attaacgcaa gaacagcaaa atattttgca
    10501 aaatctaccg cttgatcctg ccgatattca tttgattacc gcccctcgtg ggcggggcaa
    10561 atctaccctt gcagggcgac ttgcaaaagc gttacccgca caaaataagg tattaattac
    10621 cgcacggtct tattctgcat tgcccagttt ttggcgttcg gcagagcaaa aaatcccttt
    10681 tttcgcacct gacaatttaa tcgagcaagt taaaagtaaa aacatttcac ctaaccaatg
    10741 gctctttatt gatgaggcag ctagcctacc cttgccgata ctccacacac tttgtgccta
    10801 ttttgaaaaa gtggttttaa ccacaacaac ccaaaattac gaaggcacgg ggcgaggatt
    10861 tgagctgaaa ttcccggtaa tgttggataa accgtttaaa cattggacgt tatctcaacc
    10921 attgcggtgg agcgaaaatg atcctctcga agcatttgtg actgagttgc tattactttc
    10981 cgaagaaaaa tctcccctct ttagcaaaga ggggaagggg gagatttatt cgcttcgctc
    11041 acactatgtg ccgttgctaa agcaacgttc aaacgcaaag cgtttgtggc agaagcaatt
    11101 atcgaagaat ataagttatt cgaataagca tttacaggaa aaaatcgctt tctaccactt
    11161 actcgccaac gcccattaca aaaccactcc aactgactta cgccgtttat ttgatggcga
    11221 aaatcagctt ttgttttcac aacaggaaaa cgagcaactt atcggcggaa tttgggcaat
    11281 gcaagagggt ggtttagatg aaaaattaac ccaagccatt tggctagggg aacgccgtcc
    11341 gcaaggcagt ctagtggctc aatatctctg ttttcaaggg aatttaccgc aggcttgcaa
    11401 attgcattcc attcgcattt ctcggattgc ggtattgcct gaaaagcaaa atcaaggcgt
    11461 tggcaagcgg ttgatttctc agtttatttt gcaaaattcg gtagaaaaac gaccgcttga
    11521 ttttatctct gtgagctttg ggctaactac tccactccac gcattttggc aatcttgtgg
    11581 tttccaactt gtgcaaatca caccgaataa agaggcaagc agtggttatc aaagtgcaat
    11641 gatgatttac ccgatttcgg agcaaggaaa gttatttgca cagcaggcaa cccaagcctt
    11701 tgccagagat ttcgcccttc agccattttt cacggaaatt tgtgaggaat tgcaaaattt
    11761 cgaacaattt cgaccgcttg ccacacacaa tttagatgaa cgtgattgga aaaatttaac
    11821 cgcgtttgct catggcaagc ggacgttttc agcggcgtat gtgagtttaa aacggcttta
    11881 tttatcggat aaagcacgct tttcagagct ggatattttt gaacgaaaag ggcaaaaaat
    11941 ctccaaaatg gaggtggaaa attatcgaaa actcgttttg caaaacttaa acaataaatc
    12001 atttaaaata atttataggt tttattacat ttttttgtgt ttttgctctt gctaaatctg
    12061 caatttaatc gtataaattc actgtttatt tttcattttg atgaggaact ccaatgtcaa
    12121 tgtttagtca cattcaagct gctccggcag actcaatttt aggcttaggt gaagcgttta
    12181 aagcagatag ccgtgaaggt aaaatcaacc ttggtatcgg tgtttatatg accgatgaag
    12241 gcaaaacccc gattgtcaaa gcggtaaaag aagcagaaaa acgtttatta gagacggaaa
    12301 acagtaaaaa ttatttaacc attgacggtg ttcaagcatt caatgcggca acgcaagcgt
    12361 tattatttgg cgaaggggct gaagtgatta cctcaggtcg tgctaaaact gcacagagtt
    12421 taggtgggac aggggcatta cgcattgcgg cggaatttat caaacgtcat acgaatgcga
    12481 aaaatgtatg gatttcaacc ccgacttggc caaaccataa cggcattttt gatgcagtag
    12541 gcgtgaatgt aaaaggctat cgctactaca ataaagagac taacggttta gattgggaaa
    12601 atttaattgc cgatttaagc caagcggaag ccggagatgt ggtgttattg cacggttgct
    12661 gccacaaccc gaccggaatt gacccaacac ctgcacaatg ggaacagtta gcagcattat
    12721 ctgccgaaaa aggctggttg ccgttatttg actttgctta tcaaggcttt gcaaacggtt
    12781 tagaggaaga tgcttacggt ttacgagctt ttgtgaaaaa taaccgtgaa ttgttggtcg
    12841 caagctcatt ctcgaaaaac tttgggttat ataacgagcg tgtcggtgca tttaccctaa
    12901 ttgcagataa tgctgatgat gccaaccgtg ctttcactca aatcaaatcg attattcgtg
    12961 tactttactc taacccatct gctcacggtg caagtgcagt agcagttgca ttggctgatc
    13021 ctgaccttaa agcacaatgg attgcggaat tagatgaaat gcgtaaccgt attaaagaaa
    13081 tgcgtagcca attagtgcag aaattaaaag aaaaaggggc aaacaaagac ttctctttca
    13141 ttactgagca aaatggtatg ttctcattca gtggcttaac accggagcaa gtggataagt
    13201 taaaagatga gtttgcaatt tatgctgtgc gttcaggcag aatcaatgta gcaggcatta
    13261 ccagcaaaaa cattgatgct cttgccgatg cgattgtgaa agtgctttaa ttaaacctta
    13321 aagatatata gaggcataag cctgagtgct tgtgcctctt ttttatcttt taggtacaag
    13381 tgtggacact tgcaccaacg ttgattattc gtagaactag gtttccaacc aaagcataaa
    13441 atctgctacc gtatgaaatg agccgaacac cagcacaata tcattttcgt tggctgtttc
    13501 aaacaatact cggcttgcct gtgccacatt ttcatagcta caagcggttg aatttggcaa
    13561 aacgtttacc aatttcgcta acaccgcctc gcctgtttgt ccacgccaac aatctagccc
    13621 tgcacaatgc cattcatcaa taatattagc taatggctcg acaataccgg ataaatcctt
    13681 atcttctaac gcactaaaca ccgcatagat tttttgattt ggttgcttta actcccgcag
    13741 tttgtcagct aaataacgtg ctgcgtgtgg gttatgccct acatcaataa ttacttttgc
    13801 agccggttta ggttgagaaa aatgagtaaa atcggtatcc gtcagggttt gaaaacgccc
    13861 tgtcatttga gcttcattta aggcttgtcg aataatcttt tcatcaattt caaatggcag
    13921 ttgcattaat actgctaatg cggtgccggc gtttggaatc ggaataagcg ggattggtaa
    13981 atcggtaaag gatttttctt cagaatacca atgaaacaga ttattcttaa tttcaaactg
    14041 ccagtcttta ccccggtaaa aagcgtgaca atttaacgaa tcagcaatct gtttaatcga
    14101 atgagggcaa tcaggctcgc cgatgacaac cggaatattt tcacggaaaa tgccggcttt
    14161 ctcacgcccg atgtcttcac ggttatcgcc taaaaatgca acgtggtcga tatcaatcga
    14221 gataatcacg gcaaggcttg gatcaataat attagtggca tctaatcgcc cacctaatcc
    14281 gacttctaaa atcacaatat cgagctgatt ttgtttgaat agctcaaatg cagacagcgt
    14341 actaaactca aaataagtga gtgaagcggt cttttttgca ttaatttttg caaatgcttg
    14401 gacgtggtcg gcatcaggta atacttcgcc gttaattcgc acccgttcat tatagtgaat
    14461 taagtgcggt gaagagtaaa caccaacccg aaatccggct tttaataagg taacttctaa
    14521 caaacggcag gtcgaaccct tgccgttagt accggcaacg gtaatcacat aaggggcagg
    14581 cttgagtaaa tccagctctg ttgcgacctt acgaatacga tctaagccca tatcaatcgg
    14641 tttagaatgg gcttgttcta aataggaaag ccacgtttcc aaagaatccg tggctttagg
    14701 tatgcttaat gtattcattc agatatttta ttctgcgttt tctacaacca attccgccac
    14761 tttaaacggc gttggttgat tggttaattt tgcacaaaga cgggaaaggg tatcacgcat
    14821 ctctttacga tgcacgatca tatcaactgc cccgtgttct aataagaact cggcacgttg
    14881 gaaaccttcc ggcagttttt cacgtacggt ttgctcaatt acacgaggac cagcaaagcc
    14941 gattagggct tttggctcag caatattcac atcgcccagc attgccaaac ttgcggaaac
    15001 cccacctaaa gttgggtcgg ttaatacaga aataaacggt acgccctgct ctttcatttt
    15061 ggctaaaatc gcactggttt ttgccatttg cattaaagag aataacgcct cttgcatacg
    15121 agccccgccc gatgccgaaa aacagataaa cggaattttt tcagctaaag ctttttccgc
    15181 agctttcaca aattttgcac ctactacaga ccccattgaa ccgcccataa actcaaagtt
    15241 aaaggaggcc gtaaccaccg gcatattatg caatgtaccg gcaagcacga taaaagaatc
    15301 tttctcaccg gtttgttttt gcgcggcagt taaacgatct ttatatttct ttaaatcttt
    15361 aaatttcaac acatcttccg gctctaagtc ggcagcgatt tcgaccgcag aatctttatc
    15421 taacaaattc aataaacggg tgcgggcatc aatacgcata tgatgatcgc attttggaca
    15481 aacctgttgg ttgcgttgta attcttcgct atagagaact tgttcacaac ttgtgcattt
    15541 tgtccataca ccttccggca cttttgattt tgcattgctt gatgatgaag agtctctacc
    15601 taaaattctg tctaaccagc tcattattat tccttttgga ttgagttgtt gggttcacta
    15661 aatggttgct attaaagcat aatttatcgg tttttgatat gttttcttga catatcagat
    15721 cactaactat gtgtataagt tcgataaaaa aacgcaatca gaagaatgat taacatcacc
    15781 acaatttgaa ttaagcgttt tttggttaat ttttccgctg ccattgctct gctcatactt
    15841 aataaatttc cgcattataa gcgaaaaagc acgggctatg tagaatttgc aaaattttga
    15901 tcaaaaataa ccgcttgtaa aaaggaaata ctatggcaac cattgaacaa tttcttgagg
    15961 ttgtaaacca acttcgtcac cctgaacacg gctgcccgtg ggatctcaaa cagaattttg
    16021 acacgatgtt ccccacttat tggaagaaac ctacgaagta gcggaagcga ttcacaccca
    16081 agatcgcact gccttgcgtg aagagttagg cgatttatta ctgcaagtgg tatttttaag
    16141 ccaattagcg aaagaggaag gcagtttcac ttttgatgat gtagtaacgg atattcacga
    16201 taaactgatt taccgccacc ctcacgtttt tggtgatgtg aaagctacta acagcgaaga
    16261 ggccttacaa agttgggaag cacaaaaagc gaatgatgca aaacaccgta gccaagattc
    16321 tatttttaga tgatttacct tttgctttgc ctgctcttac ccgtgcaaat aagctacaaa
    16381 aacggtgtgc aaaagttgga ttcgattggg ataaccctca cgatgtgctg gcaaaagtcg
    16441 aagaagaact cgatgaagtc aaagcagaaa tcgcacaata tccggctcgc tccatcgagc
    16501 ttgctgaaga attaggcgat ctcttgtttg cgattgtaaa tttatgccgc cattatcata
    16561 ccgatgccga ggaaatttta cgcaatgcaa atctaaaatt tgaacatcgc tttcgaaaag
    16621 ttgaaaaatc tgtgaaaaaa tcgggaagaa ctgtaaaaga atgtaaatta gaagaactag
    16681 atgtaatttg gcacaaaata aaagtggtaa aataaaaagc accaattgtt atactaattt
    16741 ttacctttgt a
//
LOCUS       DANLXQ010000041        16500 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.041, whole genome shotgun sequence.
ACCESSION   DANLXQ010000041 DANLXQ010000000
VERSION     DANLXQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16500
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.041"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..895)
                     /locus_tag="PXD94_001002"
     CDS             complement(<1..895)
                     /locus_tag="PXD94_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285882.1"
                     /translation="MSKIEFEKIPNSIRKPSVLTEYNNKDAVTTLPTNEQEVLIVAPM
                     LNGEAAFTAPQKIFSDVEAENLFGKGSVAHLMVRQAIQNNPLIRLTVVGLKDHEAGIA
                     ATGQVSFTGTVTYAGVVRITIAGTAYEVAAAKGEEAQAIVARLVNVINAASYSPVVAS
                     VESETTLKLTSKAKGEISNEITLATRNTATGLTLEARAFDNGQRNALIAPALASVAGT
                     HYNVIISPFSDDENALALRSHLESVSAPIEDKPAIGVMGWRGTYATGTTLTASLNSER
                     IIVGWYKGATESNAMIAAGLGA"
     gene            complement(895..1074)
                     /locus_tag="PXD94_001003"
     CDS             complement(895..1074)
                     /locus_tag="PXD94_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDL6285883.1"
                     /translation="MLKVKAAKGVRVPVENRPHHYIEQEAVEVENTLYYQRRIADGDL
                     IVVKETKSAKETGGK"
     gene            complement(1075..1716)
                     /locus_tag="PXD94_001004"
     CDS             complement(1075..1716)
                     /locus_tag="PXD94_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HDL6285884.1"
                     /translation="MISKIERALIERLTLGLGKMVYAVAGYAGEINDEKLDVLRLPAC
                     LVSYGGSAFEAKSMGVRGKRYQATDVFVVLVITRSMRNQTAGRLGGVTSREVGVNQLL
                     GAVKTLLINQTLDGLVHPIQPKRIRTIWNNAEVKKEKLSAYAIEFEISYNECNVLDDG
                     RFPEGVEPNEQVFKQYQGKLDEPQGDLSGFNNRIFDPNNNAETAFRTEFKDGD"
     gene            complement(1713..2138)
                     /locus_tag="PXD94_001005"
     CDS             complement(1713..2138)
                     /locus_tag="PXD94_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL6285885.1"
                     /translation="MYALVDDFVLRIGEQESIELTDREMVGVVDEATLEVALADSSSQ
                     IDGYLSGRYRLPLKTVPQNLKRICCDLARYHLTSKSLVTMTEEVENRYKLCLKELENI
                     SKGVVSLAVDETNEEESATGENTVQFFNGGNRIWGRDKR"
     gene            complement(2138..2479)
                     /locus_tag="PXD94_001006"
     CDS             complement(2138..2479)
                     /locus_tag="PXD94_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285886.1"
                     /translation="MAKPKKNDNEALNTNENTPLEDKVNEQPEDKTDEQPKPPESETK
                     PDDGALINPIAFEVKLKSIHPQATYGRCGYRFNKETAVEIARKALSDEQIITLANDPY
                     LEFVPVVEGDA"
     gene            complement(2527..3444)
                     /locus_tag="PXD94_001007"
     CDS             complement(2527..3444)
                     /locus_tag="PXD94_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HDL6285887.1"
                     /translation="MFKKSELLKALDTAFRKEFSAGLKVLEPQWGTVAMKVSSSTATN
                     TYAWLGQFPKMQEWVGDRQIKNMQAQGMTIENKLFESTVAVPRTAIEDDQVGLFTPMV
                     KQAAQSAAELPDDLVFSLLKKGKTTLCYDGQNFFDTDHPVYQNVDGTGTSKTQSNITT
                     GSASGKPAFYVLDDSQAIKPLIWQERTTPEIETKFDPSESDHVFMKDQYLWGVRARGN
                     AGFGFWQLIHRVEDSELNSETLEKVLTAMRTLKGDGDKQLNIRPTTLLVPPSLEFAAR
                     KLLEAELIDGTTNTLKGVLKVVVNPFIAE"
     gene            complement(3444..4514)
                     /locus_tag="PXD94_001008"
     CDS             complement(3444..4514)
                     /locus_tag="PXD94_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HDL6285888.1"
                     /translation="MFKHKFKLNPIALSFKLNQEANGRIQLFPFGWFEAQDGRGGRWY
                     VGDENGYALADDINNTAIDLMIDYEHQTLYIAENGKGNPAAGWITKAEYISGEGLFAD
                     VKWTPQATQEIKDGVYRYLSPLFLTDGSGMVIKVLNAALTNRPALHNLQEVVAMSSQF
                     AKFLTPEEENPKMKELLIKLFALSANATDEQITEKLTALEKAKGDSPVALSDVYTELA
                     KEKSQVVALSAKVNHPDPAKFVALSDLQAVQTELNGLKQQINDKERDALIQTALSDGR
                     LLPAQKAWAEKLGKQDLVALSDFLKTVTPNPALASTQSGGEDPNKGTEKVALSAAEIA
                     AAKSLGLSEAAYIEKYKTEESK"
     gene            complement(4773..5189)
                     /locus_tag="PXD94_001009"
     CDS             complement(4773..5189)
                     /locus_tag="PXD94_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL6285889.1"
                     /translation="MIEIQIHGIDEIIATLNRIAAKTANKTDLMRNIAGKMESAVLTN
                     FDEGGRPKWLGIKHRQGTPLVDTENLMGSITADYNNDSAIVGTNEPYAAIHQFGGMAG
                     RGRKVKIEARPFLQLTSQDRQDILDDVQAYWQKLLD"
     gene            complement(5340..6639)
                     /locus_tag="PXD94_001010"
                     /pseudo
     CDS             complement(5340..6639)
                     /locus_tag="PXD94_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(6626..8251)
                     /locus_tag="PXD94_001011"
     CDS             complement(6626..8251)
                     /locus_tag="PXD94_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDL6285890.1"
                     /translation="MSKKKHNKQIKPNLNPIKNQELQTDLAEITATGRVLSDHPSNFI
                     TPAKMKAIFEDTENGDITAQHELFMDIEERDSSIFANIQTRKRAALGVDWAIVAPRNA
                     TPQEEKLRDEVDELFYQLGNLEDLVMDCMDAVGHGFAALEIEWAFNGKLWYPNAFIHR
                     PQSWFKWDKHDNLLLKTRQNTEGEPLREFGWVVHTHKSRSTQAARNNLFRSLAWLYMF
                     KHYSIHDFAEFLELYGMPIRIGKYGAGATPNEKQTLKRALAEIGHNAAGIMPESMSIE
                     LHNAANAGGAAGNNPFLQMVDWCEKSIARLILGQTLTSGADGKSSTNALGNVHNEVRR
                     DLLVSDVKQLGQTFTQQIILPFLQINFGNIDPTRIPSFEFDTKEPADLALFAESLPKL
                     VDIGMPIPVQWAMDKLGIPEAQENEAVLGRSAPPQAVGLSANFPQKMTACECGCGGKV
                     HALSAQTKTGIKEQDALDDLADEAFSVPDFNAQFDPITKKAVAVVMACSSYDEAAEKL
                     AAAYPDLVSEEHDRYLANALFLADLLGAANAES"
     gene            complement(8320..9945)
                     /gene="terL"
                     /locus_tag="PXD94_001012"
     CDS             complement(8320..9945)
                     /gene="terL"
                     /locus_tag="PXD94_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL6285891.1"
                     /translation="MKIKEFEKELEELRRQLQQNIEASFEGWDDKPQAISERRQKVLD
                     PVHGFEYFVQAYFPHYVRSEHKSQLHHYLFENLPLSVSDLSKSVRQAIAAPRGEAKST
                     ICTQLFPLWCLVRDLKRYIIIAMDTKEQAYSMLEAIKVEIESNPRLRIDFPEVAQGKV
                     WRAGVIMTAKKQKVEAVGAGQKLRGRRHGAYRPDLVVLDDIENDESVETPAQRNKLHN
                     WVLKAVLKLGAAGEKFDVIYVGTILHYDSVLSRILNTKGWRRVRFKAILRFPGNMALW
                     DEWENIYLSEEGDDDTMSDLFYQQHKAEMDKGALVSWLARPILYLMKIRASDGHSAFD
                     SEYQNDPVSGDDAIFANSIQYWTELPDDLIYFGALDPSMGKAGASRDPSAILVGGYHR
                     ESGKLYVVEAQIKKRLPDLIIEDVIRLHSQYNCHRWFVETVQFQEFLQTELVKRSAQR
                     GKPVPATATKPNSDKMLRIESLQPHIANGLILLHRSQSTLESQLRHFPKADHDDGPDA
                     LEMLWRNALSSSAPIEWESVDDFDGEYRSKWRH"
     gene            complement(9949..10095)
                     /locus_tag="PXD94_001013"
     CDS             complement(9949..10095)
                     /locus_tag="PXD94_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285892.1"
                     /translation="MAKKKQTVVHKHYSFRWIVQLACIFGAIKLMLNGTDGWGWLLLI
                     AACM"
     gene            complement(10095..10595)
                     /locus_tag="PXD94_001014"
     CDS             complement(10095..10595)
                     /locus_tag="PXD94_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HDL6285893.1"
                     /translation="MAHDVEVQKAVRQAYVFDRLSLEMAAERAGVSFGTARRWKAAAE
                     KNGDNWEKARDVQAMASGGIENIAQGLLAGFLIKYRTLMTELEENTEMPTAAKVEALA
                     ALADSFAKMTASSKKLLPETSAMATAMRAIEMMANIVKTKKPHLLPDFLEMLDELEVQ
                     FKKEFK"
     gene            complement(10603..10857)
                     /locus_tag="PXD94_001015"
     CDS             complement(10603..10857)
                     /locus_tag="PXD94_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285894.1"
                     /translation="MSRKKQKQTDSEKLDLILGGLNELNDKVDKQNEEIERLHREIQA
                     TNRLVNEMARKNRRDAVVAGGLGGGIVAVGFELLRLKFGM"
     gene            complement(10857..11114)
                     /locus_tag="PXD94_001016"
     CDS             complement(10857..11114)
                     /locus_tag="PXD94_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285895.1"
                     /translation="MIDEKLFQMLISFVVAPLVAFVGKVIFDRINRNEQTVKELKAEM
                     ESKYQSKELAHEVNRGIKEKLDDILDSLKEVNQKLDKKADK"
     gene            complement(11107..11262)
                     /locus_tag="PXD94_001017"
     CDS             complement(11107..11262)
                     /locus_tag="PXD94_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285896.1"
                     /translation="MKLIKKLRQRWQQWRFYRRRPDLAELHNVVRAAVAQGNQHPVGR
                     AKRGRYD"
     gene            complement(11277..11636)
                     /locus_tag="PXD94_001018"
     CDS             complement(11277..11636)
                     /locus_tag="PXD94_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL6285897.1"
                     /translation="MITQQIAGVVGVVSTILAYAFYKSWQLKQERKLTATLKAEKQQQ
                     AVEIQQKNAEVKNAKIQQKHVSETRRIQPSAIDEQLHQHGYFRDDNGLHGVRSDLPEP
                     CGHNGNETPSACTQSDL"
     gene            complement(11633..11878)
                     /locus_tag="PXD94_001019"
     CDS             complement(11633..11878)
                     /locus_tag="PXD94_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL6285898.1"
                     /translation="MKQFLLDLITNKDGTASTTGFIQFMSWLVLSGILIHAYLYDKPF
                     ISDWWFAYAGICVLGSPATKGVVSALKQKERSREAEE"
     gene            complement(11881..12414)
                     /locus_tag="PXD94_001020"
     CDS             complement(11881..12414)
                     /locus_tag="PXD94_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL6285899.1"
                     /translation="MTEPIKNKATIADFNLAFDRLIGHEGGYVNDPKDPGGETNWGIT
                     IHTARANGYTGSMRAMSRADAKTIYLKAFWQRYNCEQFPPELAFQFLDACVNHGSGNA
                     SRMLQRAVGVADDGIIGKVTLAAIERYSVADVGLLFQAERLEFYTKLKHFDRFGRGWI
                     NRMAGNLRYFAKDTVEE"
     gene            complement(12500..12925)
                     /locus_tag="PXD94_001021"
     CDS             complement(12500..12925)
                     /locus_tag="PXD94_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HDL6285900.1"
                     /translation="MSEFESVEHYLPETVKEIVGVIGLPATEKLIKAFGGFSFQFSNG
                     KLYFNKLKEVLGQDDAVKLQAYMGACEVYLPRCETALRMLRNQQIYADYCQLTEQGGL
                     SGRLAIMQICPKYSVCDRVAWEAVRYYQRKHTVSQATLF"
     gene            complement(12996..13211)
                     /locus_tag="PXD94_001022"
     CDS             complement(12996..13211)
                     /locus_tag="PXD94_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6285901.1"
                     /translation="MIENPEFGYTPSNLKMLRKHHNLTQTEVANILGVGQRIVMRWET
                     ELSSTAARSDMPYTKWAELLKNLQKNF"
     gene            complement(13230..13622)
                     /locus_tag="PXD94_001023"
     CDS             complement(13230..13622)
                     /locus_tag="PXD94_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285902.1"
                     /translation="MTDYQKLLNALLNDADEDCFAVYGLRATTDVHQVGDELGNSFVW
                     VDGEKTDEELDGICTMGIQNADEAGLLNAIKNLGRDACKKFDVEFKGFQSYCGQNFIL
                     VKGDSARGGEDKGESIIRNPIVVAVFNS"
     gene            complement(13748..14095)
                     /locus_tag="PXD94_001024"
     CDS             complement(13748..14095)
                     /locus_tag="PXD94_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285903.1"
                     /translation="MKELLLTTLCLLSLPAIAMTEKAEQETANALVSGDYQQLRNVAY
                     GMETGSFGHDHNPIAACALRRVILLVNSDKVDMTDFNNEAIACRKIEVTDNQQAWETA
                     FTIAKSISATKKK"
     gene            complement(14184..14609)
                     /locus_tag="PXD94_001025"
     CDS             complement(14184..14609)
                     /locus_tag="PXD94_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDL6285904.1"
                     /translation="MQPQTRKQMIQKIHIGKNELKLDDNAYKMLLLEAVDKPSCSMMT
                     DSELMTVLQTMKAKGFVVKSKKYGKRPTASKTAPHRQGLINKIGALLYQSQKPWDYAH
                     AIAKRSFGIERVQWLTDDQLHKLVQILASYNHRHGMSVK"
     gene            complement(14593..15147)
                     /locus_tag="PXD94_001026"
     CDS             complement(14593..15147)
                     /locus_tag="PXD94_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285905.1"
                     /translation="MKLCKCPICHSDISFEALIEDDAGRELLNTIVNIGGNCGRAVVA
                     YLGLFKPQKNSLANSRALKIVQSLLELYPPSNVLEKALMDTVEQVRRNRRETGRCEPL
                     ANHNYLKKVYESVKPQFAVVRSEAKAVSSLEQQQRQADQKKHDAVMYVERMKQMGQLE
                     AVKNTESYQIWLKWQEEKQNATAN"
     gene            complement(15147..15317)
                     /locus_tag="PXD94_001027"
     CDS             complement(15147..15317)
                     /locus_tag="PXD94_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285906.1"
                     /translation="MEYQKIPEYRLCKKKSGEIVLQQLVICELTPATWHNPYPDSRTY
                     WVDLETIEEGTE"
     gene            complement(15388..15963)
                     /locus_tag="PXD94_001028"
     CDS             complement(15388..15963)
                     /locus_tag="PXD94_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HDL6285907.1"
                     /translation="MKFRFFKGDITQEPLKSIYADYQVSKEERKDKLNAILEKYPFND
                     GLISRSGWFHRIVIGIACKATNISQVLDVKGFKVGKFNDEFYLVKPDKRYEKGKELAE
                     DFKAINEIYEKHRDFSPYILKRLNMLYFVSDFSYSPTSGRSYLAVAGICENTLLVEMP
                     MPIDKNDEPFPEIAEGLVEIKESEFLAIQGK"
     gene            complement(15986..16168)
                     /locus_tag="PXD94_001029"
     CDS             complement(15986..16168)
                     /locus_tag="PXD94_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285908.1"
                     /translation="MTEQEKNELNSQLNEALMQIIQAQKYLKQSDFIRSGVYLGTVQD
                     LLPKVHLKLLTANRKH"
     gene            complement(16235..16381)
                     /locus_tag="PXD94_001030"
     CDS             complement(16235..16381)
                     /locus_tag="PXD94_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL6285909.1"
                     /translation="MKTEQKATEFDRYKHYAEKAAEAERKGNYAEAQDHWKVAKLSAK
                     KTAN"
BASE COUNT         4339 a         4071 c         3085 g         5005 t
ORIGIN      
        1 ccgcaccaag ccctgccgca atcattgcat ttgattcggt tgctccctta taccagccta
       61 caataattcg ctcactgtta aggcttgcgg ttaaggttgt gccggtggca taagtgccac
      121 gccagcccat tacaccaata gccggtttat cttcaatcgg ggcagaaacc gattctaaat
      181 ggctacgcaa ggcaagggca ttttcatcat ctgaaaacgg cgaaataatc acgttataat
      241 gcgtaccggc aacactcgcc aacgccggag caattagggc attacgctga ccgttatcaa
      301 acgcacgagc ctcaagggtt aaacctgttg ctgtattgcg tgttgcaagc gtgatttcat
      361 tgctgatttc gccttttgct tttgaggtca gtttaagtgt cgtttcactt tccacacttg
      421 ccaccacagg gctataactc gccgcattaa tcacattaac taaacgagcc acaatcgcct
      481 gtgcttcttc gccttttgcg gctgcaactt cgtaagctgt gccggcaatc gtaatacgca
      541 ccacaccggc ataagttact gtgccggtaa aactaacttg ccctgttgct gcaatacctg
      601 cctcgtggtc ttttaagccc accaccgtta aacggattag cggattattt tggattgctt
      661 ggcgaaccat taaatgagct accgagcctt taccaaataa attttccgcc tccacatcgc
      721 taaaaatctt ttgcggagca gtaaaagcag cctcaccgtt tagcattggt gcaacaatta
      781 gcacctcttg ctcattggtc ggcaaggtgg ttactgcatc tttgttgttg tattcggtca
      841 gcacacttgg tttgcgaata ctgttcggga ttttctcaaa ttcaattttt gacattattt
      901 gccccctgtt tctttagcgg atttggtttc tttgacgaca atcaaatcgc catccgcaat
      961 gcggcgttga tagtaaaggg tgttttcaac ctctactgcc tcttgttcaa tatagtgatg
     1021 cggtcggttt tctaccggca cacgcacccc tttagcggct ttcactttta acatctaatc
     1081 accatcctta aattctgttc taaatgccgt ttcggcattg ttattcgggt caaaaatccg
     1141 attattaaag cccgataaat cgccttgcgg ttcatctagc ttgccttgat actgcttaaa
     1201 gacttgctca ttcggctcaa ccccttcggg gaatctacca tcatcaagca cattgcactc
     1261 gttgtagctg atttcaaact caatagcata agcagagagt ttctctttct tcacttccgc
     1321 attattccaa atcgtgcgga ttcgcttagg ctggataggg tggactaaac cgtctaaagt
     1381 ctggttaatc aataaggttt tcaccgctcc cagcaactga ttaaccccca cctcacgact
     1441 tgttacccca cctaaacgcc cagcagtctg atttcgcatt gagcgagtaa taaccaacac
     1501 cacaaacaca tcagtggctt gatagcgttt acctcttact cccattgatt tcgcctcgaa
     1561 cgccgagcca ccataagaga caagacaagc aggcaaacgg agaacatcta acttctcatc
     1621 gttaatttcc ccggcatacc cagcaacggc atacaccatt ttgcctaagc ccagcgttaa
     1681 acgctcaatc aaagcacgct caattttgct aatcatcgct tatcacgccc ccaaatacgg
     1741 ttgcccccgt taaaaaactg cactgtattt tcgccagtag ccgattcttc ctcattggtt
     1801 tcatcaaccg ctaacgacac tacgccctta gagatgtttt ccagctcttt taagcaaagt
     1861 ttgtagcggt tttccacctc ttcggtcatc gttactaatg atttgctggt taaatgataa
     1921 cgagccaaat cacagcaaat acgctttaaa ttttgcggta cagtctttaa tggcagacgg
     1981 taacgcccac ttaaatagcc atcaatttgg ctggaagagt ccgctaaagc tacttcaaga
     2041 gtcgcttcat caaccacgcc taccatttca cggtcggtta gctcaatgga ttcctgttcg
     2101 ccaattcgca aaacgaaatc atcaaccaac gcatacatta cgcatcgccc tcaacaacag
     2161 gtacaaactc caaataaggg tcgttggcta aggtaataat ttgttcatca ctcaaggctt
     2221 tacgagcaat ttctaccgcc gtttctttat taaaacggta gccacaacgc ccataggtgg
     2281 cttgtgggtg gatagatttg agcttaactt caaaagcaat aggatttatc aaagcaccgt
     2341 catcaggctt agtttcactt tcaggcggtt taggttgttc atccgtttta tcttcaggct
     2401 gttcattcac cttatcttca agcggtgtgt tttcgtttgt gttcaaagct tcattgtcat
     2461 ttttctttgg ttttgccatt ttgctaccct ctcaaaatgg gcgtattgct acgcccaaca
     2521 ttaaacttat tcagcaataa atggattcac taccactttt aacacacctt ttaaggtgtt
     2581 ggttgtgccg tcaattaatt ctgcttcgag taatttgcgt gcagcaaact caagcgaagg
     2641 tggcacaagc aaagtggtcg ggcgaatatt taactgctta tcgccatcgc cttttaaggt
     2701 gcgcattgca gttaatactt tttcaagggt ttcactattt aactcactat cttcaacacg
     2761 gtgaattaac tgccagaaac caaaaccggc attaccacga gcacgcacac cccataaata
     2821 ttggtctttc ataaagacgt ggtctgactc acttggatca aacttcgttt caatttcagg
     2881 tgtagtacgt tcttgccaaa ttaacggctt aatggcttgg ctatcatcta gcacatagaa
     2941 cgcaggctta ccgctggcag agcctgtggt gatattactt tgtgttttac ttgtgccagt
     3001 tccatcaaca ttctgataaa cagggtgatc ggtatcaaag aagttctgcc cgtcatagca
     3061 aagggttgtc ttaccttttt tcaacaaact aaataccaaa tcatcaggta attcagccgc
     3121 actttgtgcc gcttgtttca ccattggggt aaataagccc acttggtcat cttcaatggc
     3181 agtacgaggc acagcaacgg ttgattcaaa taatttattc tcaatcgtca ttccttgtgc
     3241 ttgcatattt ttaatttgac ggtcgcccac ccactcttgc attttcggga attgtcccag
     3301 ccaagcatag gtattagtag cggtgctgga gcttaccttc attgcaaccg ttccccattg
     3361 tggttcaagc acttttaaac cagcactaaa ttctttacga aatgcagtat caagtgcttt
     3421 taataattca gattttttaa acattacttg gattcctctg ttttatattt ttcaatgtaa
     3481 gctgcttcgc ttaaaccaag cgattttgct gctgcaattt ccgcagcaga taacgccact
     3541 ttctccgtac ctttgttcgg gtcttcaccc cccgactgcg tacttgctaa agcggggtta
     3601 ggtgttaccg ttttcaaaaa gtcggataaa gccactaaat cttgcttacc gagcttttcc
     3661 gcccacgctt tttgagccgg caataaccga ccgtcagaaa gagcggtttg aattaacgca
     3721 tcacgctctt tatcgttgat ttgctgtttt aagccgttca gctcggtttg cactgcttgc
     3781 aagtcgctta acgccacaaa cttcgccggg tcagggtgat tcactttggc agagagtgca
     3841 accacttggc ttttctcttt tgccagctcg gtataaacat cacttaacgc caccgggctg
     3901 tcgcctttgg ctttctcaag tgcggtcagt ttttcggtaa tctgctcatc ggtcgcattt
     3961 gccgacaatg caaataattt aattaagagt tctttcattt ttggattttc ctcttcgggg
     4021 gttaaaaatt tggcaaattg gctagacatc gcaaccacct cttgcagatt atgcaaggca
     4081 gggcggttag ttaaggcggc attaagtacc ttaattacca ttccgctgcc gtctgtcaaa
     4141 aacagtggcg agagatagcg ataaacgccg tctttaatct cttgggtcgc ttgtggtgtc
     4201 cacttaacat ccgcaaacaa gccttcgccc gagatatatt ccgccttggt aatccaaccc
     4261 gcagcagggt tgcctttgcc gttctcggcg atgtacaggg tctgatgctc ataatcaatc
     4321 atcagatcaa tcgcagtgtt gttaatatca tcggctaacg catagccatt ctcgtcacct
     4381 acataccaac gtcctccacg cccgtcttga gcctcaaacc aaccaaaagg gaaaagttga
     4441 atacgtccat ttgcctcttg gttgagttta aaactcaatg caatggggtt tagtttgaat
     4501 ttatgcttaa acacaaaaac acctcaaaac aaattaatga ggctaaagaa tagaagattg
     4561 agaaaaaaga gtaacgggga gcaacttcca caagttgcaa attttcagaa aaaaacaacc
     4621 gcttgtattc agcgaggaat gtaacccatt ttaaaacgct ttaaatccgt tttaaaaaat
     4681 tttaaaaaag ttggaatgat aaatcaccat aaaaaaaaca aaaacgccac agagagcgat
     4741 ttaggggcta tctgtggcga ttatcttatc gcttaatcca gcagtttttg ccagtaggct
     4801 tgcacatcgt ccaaaatatc ttgcctgtct tgcgaggtta attgtaaaaa aggtcgggct
     4861 tcaattttta ctttacgccc acgcccagcc ataccaccga attgatgaat agcagcataa
     4921 ggctcatttg tgccaacaat cgccgaatca ttattataat cagcagtaat gctgcccatt
     4981 aaattttccg tatcaactaa cggcgtgcct tgccgatgtt ttatacccag ccatttcggg
     5041 cgaccgccct catcaaaatt ggtcagtacc gccgattcca ttttgccggc aatattccgc
     5101 atcaagtctg ttttatttgc cgttttagca gcaatacggt taagcgtggc aataatttca
     5161 tcaatgccgt ggatttggat ttcaatcatt tttaaccttg caagttgatt tttaaaataa
     5221 acaggggtat attcctttta cggttattag aaaagcggtg ataatctccc tgccgcagcc
     5281 tatttgagat ggcaagtcct gctcaaatga ccgagagcgt gtgtaagtga ccgggagcct
     5341 tacctaataa ccgtattttt caacagcagc ttatccaatt ctttttgtcc aatctccctt
     5401 gttgatacca gaaataattc accaaactct tctaagtatt tcaccacaac aagaatacga
     5461 taatgctcaa agtttcgtat aaagctgaaa tttttgcctt cttgcaaaat atgattaggc
     5521 tgaaaaatta catcaggaat atttgcatac acatcaaacc caaaatcctg cccctcacgg
     5581 ctataaaact gcttaatcag tgtgtcatcc gaaagccata ccgtgccgac tttactgtcg
     5641 attaagcgtt gtgtttcctc acttaaccga ccagcagcaa atttaaagtt ttgattaagg
     5701 gcatttcgca ctttaaccat ctcatcaggc gagagttttt tcgccccatc tttggcaagt
     5761 gccacagcct cttctaactt gttaaacgcc tgcttaaatt caccaccgct catttctgct
     5821 ttggtaaatt ggtaagccag cttttcaggg tataaatcca aattcggctt ataggttaaa
     5881 cgccccacat tataatcaaa acctttatca gccaatcgca ccgtaccatc agccaattta
     5941 aagccaatgg tttcctcttg gttgcctaac ttatctttcg gtcgttttgc cttcactaaa
     6001 aacggctcgc tactgccgac ttcgtcaatc cctttacgct ttaaatcccg ctcacttaac
     6061 gcaatcacgg tacaacggca attaaagccg ttcggcggat aaaaagtcgc ccaaaacgga
     6121 tcgtcataac gataaatctt accctgtaat gcttggtgag cagggcgggt gcgttggtcg
     6181 cctaccgccg aatattgcca ataagggcga ttatccacat tatccatata ccgctgataa
     6241 cgagccgcag aataagcctg ttgcatattg gtacgataaa tcgtattcaa gcggcgaggc
     6301 gtgccgaaat gctcgccggt tttcgggtcg gcaagcagtt tgccatcaat gccacgacta
     6361 atcgtcgtgt ccatacaccc agcctttccg ttcaaattct gccgtaagcg ttttcttcca
     6421 ttcattaaag gatttcccct cacgccgtgc tttttccatc gactcataaa tatcacgggt
     6481 catttcaagg ctagtgagct tgctaatggt ggcggctcgc cccaatgcag aatcaaataa
     6541 ttcctgctta aacactttat tcgcaagcaa cttcttctgc cgtaaaaact caatcgcctg
     6601 cttaggctct agtccaatgg caaaattaac tttctgcatt cgcagcccct aataaatccg
     6661 ccaaaaataa cgcattcgct aaatagcggt cgtgttcttc actcactaaa tcagggtagg
     6721 ctgccgccaa tttttctgct gcctcatcat aagagctaca cgccattacc accgccaccg
     6781 cctttttcgt gatggggtca aattgggcgt taaaatccgg cacggaaaaa gcctcgtctg
     6841 ccaaatcatc taaggcatct tgctctttaa tgcccgtttt ggtttgggcc gacaaagcgt
     6901 gaaccttgcc cccacaaccg cattcgcaag cggtcatttt ttgcggaaaa tttgcggata
     6961 accccaccgc ttgtggcggg gcagaacgcc ctaacaccgc ttcattttct tgagcttcgg
     7021 gaataccgag tttatccatc gcccactgca ccggaatcgg cattccaata tccaccaatt
     7081 tcggcaagct ctcggcaaat aacgccaagt cggcaggctc tttggtatca aactcaaaac
     7141 tcggaatgcg ggttgggtcg atattgccaa aattaatttg cagaaacggc agaataattt
     7201 gttgggtaaa cgtctgcccc agctgtttca cgtccgacac caacaaatca cgccgcactt
     7261 cgttatgcac attgccgagt gcattggtag agcttttgcc gtctgcaccg ctggttaagg
     7321 tttgccccaa aatcaagcgg gcgatagact tctcacacca atccaccatt tgcaaaaacg
     7381 gattattgcc ggctgcaccg cctgcattag cggcgttatg caactcaata ctcatcgact
     7441 ccggcataat tccggcagcg ttgtgtccga tttccgctaa tgcacgttta agcgtttgtt
     7501 tttcgtttgg tgttgcccct gcaccgtatt taccaatgcg gataggcata ccatacagct
     7561 ccaaaaactc ggcaaagtcg tggatagagt agtgtttaaa catatacagc caagccagcg
     7621 agcggaataa attattgcgt gccgcctgtg tggagcggga tttatgcgta tgcaccaccc
     7681 agccaaattc tcgtaatggc tcaccttcgg tattttgtcg ggttttcagc aacagattgt
     7741 cgtgcttatc ccacttaaac cacgattgag gacggtggat aaaggcgttc ggataccaca
     7801 atttaccgtt aaacgcccat tcaatttcga gggcagcaaa accgtgtccc acagcgtcca
     7861 tacagtccat caccaaatct tcaaggttgc cgagctgata aaataactca tcaacctcat
     7921 cacgcagttt ttcttcctgc ggtgtggcat tgcgaggggc aacaatcgcc caatctacgc
     7981 ctaatgcggc acgcttacgg gtttggatat tggcaaaaat agagctgtcc cgctcctcaa
     8041 tatccataaa taattcgtgt tgagcggtaa tatcgccatt ctccgtatct tcaaaaatcg
     8101 ctttcatttt agcaggcgta ataaaattgc tcggatggtc ggagagtacc cgacctgtag
     8161 cggtaatttc ggctaaatcc gtctgcaatt cttgattttt aatcgggttt aaattcggtt
     8221 taatctgctt gttatgcttc tttttgctca taaattttcc ttttcagagc ttagttacgc
     8281 tcaatatagt ccccctcttt agcaaagagg ggagttacat taatgccgcc acttactgcg
     8341 atactcaccg tcaaaatcat ctacactctc ccactcaatc ggggcagagc tgctgagtgc
     8401 atttcgccat aacatttcca acgcatccgg accatcatcg tgatcggctt tcggaaaatg
     8461 gcgaagctgg ctttcaagcg tagattgcga gcggtgcaac aaaatcaaac cgttggcaat
     8521 atgcggttgc aagctctcaa tccgtaacat cttgtcactg ttcggcttgg ttgccgttgc
     8581 cggcaccggc ttaccacgct gggcagaacg cttgaccagt tccgtctgca aaaactcctg
     8641 aaattgcacc gtctccacaa accagcggtg acagttatat tgcgaatgca gtctaatcac
     8701 gtcctcaata attaaatccg gcaatcgttt tttgatttgt gcctcaacaa catagagctt
     8761 gccactctca cgatgatagc caccgaccaa aattgcagat gggtcacggc ttgccccagc
     8821 tttccccatt gaggggtcta acgccccgaa ataaatcagg tcatcaggca gttccgtcca
     8881 atactggata ctgttggcaa aaatcgcatc atcacccgag acagggtcat tttgatactc
     8941 ggagtcaaat gctgagtgcc catcactagc acggattttc atcaagtaga ggatagggcg
     9001 agctaaccaa gagactaacg ctcctttatc catttctgct ttatgctgtt gataaaagag
     9061 atcagacata gtgtcatcgt ccccttcttc cgagaggtat atattttccc attcgtccca
     9121 taacgccata ttgccgggaa agcgtaaaat ggctttaaaa cgaactcgcc gccaaccttt
     9181 tgtatttaaa atacggctaa gcacactgtc ataatgtaga attgtgccga catagataac
     9241 atcaaacttt tcacctgcag caccaagttt taaaactgct tttaaaaccc agttatgcaa
     9301 tttattacgc tgtgcaggtg tttctacgct ttcatcattc tcaatatcat caagtactac
     9361 taaatccgga cgataagctc cgtggcgacg accacgcaat ttttgcccag cccccacagc
     9421 ttcaactttt tgttttttag ccgtcataat gacacctgca cgccaaacct tgccttgtgc
     9481 aacctcgggg aaatcaattc ttaaacgagg gttagattca atttccactt taatagcttc
     9541 aagcatactg tatgcctgtt ctttggtgtc catcgcaata atgatgtatc tttttaaatc
     9601 acggactaaa caccacagcg ggaataattg cgtacaaatc gttgattttg cctcgccacg
     9661 aggggctgca atcgcttggc ggactgactt agaaagatca cttaccgaaa gcggtaaatt
     9721 ctcaaagaga taatgatgca actgtgattt atgttcagac cgcacatagt gtggaaaata
     9781 agcctgcaca aaatactcaa agccgtgaac agggtctaaa accttttgac ggcgttcgct
     9841 aatggcttgt ggtttatcgt cccaaccctc aaaactcgct tcgatatttt gttgtaactg
     9901 tcgccgtaat tcttccagtt ctttttcaaa ttctttgatt ttcataaatt acatacaagc
     9961 tgcgataagc aacaaccagc cccaaccgtc tgtaccgttg agcattagtt ttattgctcc
    10021 gaaaatacaa gcaagttgga caatccaacg gaaagagtaa tgcttatgca ctacagtttg
    10081 ttttttctta gccattactt aaactccttt ttgaattgca cttctagctc atctaacatt
    10141 tctaaaaagt caggcaataa atgcggtttc ttggttttga cgatattcgc catcatctca
    10201 atcgcacgca tagcggttgc cattgcactt gtttcaggca atagtttctt gctcgatgcc
    10261 gtcatcttcg caaacgaatc agccagagca gctaatgcct caaccttagc tgcggttggc
    10321 atttcggtgt tttcctcaag ctccgtcatc aacgtgcggt acttaattaa aaaaccggct
    10381 aacaagcctt gagcgatatt ctcaatacca ccgcttgcca tcgcttgcac atcacgagct
    10441 ttttcccagt tatcaccgtt cttttcagca gccgctttcc aacgccgtgc cgtgccgaat
    10501 gatacccctg cacgttctgc tgccatttcg aggcttaaac ggtcaaacac ataggcttgc
    10561 cgaaccgctt tttgtacttc aacatcgtgt gccatacgcc ctctacattc caaatttcag
    10621 gcgtaacaac tcaaaaccca cagcaacaat gccaccaccc aagccaccgg caactacagc
    10681 atcacggcgg tttttacgtg ccatttcatt cactaaacga ttagtcgctt ggatttcacg
    10741 gtgtaagcgt tcgatttctt cgttttgttt atccacctta tcgtttagct catttaatcc
    10801 acctaaaatc aggtctaatt tttccgaatc ggtttgcttt tgttttttac ggctcattac
    10861 ttatccgcct ttttatcaag tttttggttt acttctttga gcgagtctaa aatatcgtcc
    10921 agcttttcct taatccctct attcacttcg tgggcaagct ctttggattg atatttgctc
    10981 tccatttctg cttttagctc ttttaccgtt tgctcattgc ggttgatacg atcaaatatg
    11041 accttgccta caaacgccac taacggagca actacaaaag agatcagcat ctgaaacagt
    11101 ttttcatcaa tcataacgcc cccttttggc tcgcccgacc gggtgttgat taccttgtgc
    11161 caccgccgca cgcaccacgt tatgcaattc tgcaaggtca ggacggcggc gataaaaacg
    11221 ccattgttgc caacgctgtc tgagtttttt aattaacttc attctggcaa acttcctcat
    11281 aggtcagatt gtgtgcaagc acttggcgtt tcgtttccat tgtgtccgca cggctcgggt
    11341 aaatcagacc gaacgccgtg caacccgttg tcgtcacgga agtaaccgtg ttggtgcaac
    11401 tgctcatcaa tggcactagg ctgaatacgg cgagtttcac taacgtgttt ttgctgaatt
    11461 ttggcatttt taacctctgc atttttttgt tgaatttcga ccgcttgttg ctgcttttcc
    11521 gcctttaatg tagcggtaag tttccgctcc tgctttaatt gccagctttt ataaaaggca
    11581 taggctaaaa ttgtgcttac tacaccaacc acacctgcaa tttgttgagt aatcattctt
    11641 cagcctccct tgagcgttct ttttgtttta acgcacttac cacccctttt gtggctgggc
    11701 tacccaatac acaaatacca gcataagcaa accaccagtc ggaaataaac ggcttatcgt
    11761 aaaggtaggc gtgaattaaa atgcccgata acactaacca gctcataaac tgaataaagc
    11821 cagtggtgct ggctgtgccg tctttattgg taattaaatc aagtaaaaat tgcttcacga
    11881 ttattcctcc accgtgtctt tggcaaaata acgcaaatta cccgccattc ggttaatcca
    11941 acctctacca aaacggtcaa agtgtttgag tttggtgtaa aactcaaggc gttctgcctg
    12001 aaataacaag cccacatccg caaccgaata acgctcaatc gcagccaaag tcactttacc
    12061 gataatgccg tcatcagcca cacctaccgc tcgctgtaac attcggctag cattgcccga
    12121 accgtggttt acacacgcat ctaaaaactg aaaagccaac tcaggcggga actgctcaca
    12181 gttataacgt tgccaaaagg ctttgaggta aatagttttc gcatccgcac ggctcattgc
    12241 acgcatagag ccggtatagc cattcgcacg ggcagtatgg atagtgatac cccaattcgt
    12301 ttcgccaccg gggtctttcg ggtcgttcac ataaccgccc tcgtgtccga taagacggtc
    12361 aaatgcaaga ttaaagtcag caatagttgc cttgtttttg ataggttcag tcataaaaaa
    12421 agcctcttta aattcaatga tgatttaaag agactttaat gatttagagc aacggggagc
    12481 aggcgaaagg attccacact taaaacaagg tagcttgcga aacggtatgc ttacgctgat
    12541 aatatcgtac tgcctcccac gcaacacgat cacacacgct gtatttaggg caaatttgca
    12601 taatcgcaag cctgccactt agccctcctt gctcggttaa ttgacaataa tcggcataaa
    12661 tctgctgatt acgcaacatt cttaatgcgg tttcacaacg tggcaaatag acctcgcaag
    12721 ctcccatata agcctgtaat ttcactgcat catcttgccc taatacctct ttgagcttat
    12781 taaaatagag ttttccgtta gaaaattgga atgaaaagcc accaaaggct ttgattagct
    12841 tttcagttgc cggcaaacca atcacgccca caatttcttt caccgtttcc ggcaaataat
    12901 gttcgacact ttcaaattca gacataaaaa atccccttga ataatttcca cgaaagccat
    12961 tatccaaggg ggttattcaa atctgtaata cttacttaaa aatttttttg caaatttttt
    13021 aaaagctcag cccattttgt gtacggcata tctgatctag ctgcggttga tgacagttct
    13081 gtctcccacc gcattactat tctttgcccg acccctaata tgttagctac ctctgtttgt
    13141 gtgaggttat ggtgctttct tagcattttt aagttgcttg gtgtgtatcc aaactctggg
    13201 ttttcaatca ttcacctccc tccattaaac tagctattaa atacagcaac aacgataggg
    13261 tttctaataa tgctctcgcc tttatcttct ccgcctcttg cactatcgcc tttaactagg
    13321 ataaagtttt gcccacagta tgactggaaa cctttaaatt caacatcaaa ttttttgcaa
    13381 gcatcacgac ctagattttt aatagcattt aacaaacctg cttcatctgc attttggatt
    13441 cccattgtac aaatgccgtc taattcttca tctgtttttt caccatcgac ccaaacaaaa
    13501 gaatttccta attcatcgcc aacttgatgc acatctgtag ttgctcttaa accataaaca
    13561 gcgaagcagt cttcatcggc atcgtttagt aaagcattta aaagtttttg gtaatctgtc
    13621 attttaattt ctcctgtttg gctatgggtg cattatagta atataattac ctattgcaag
    13681 tgatttttcg tttttttctt aaattttttt atgtaaataa aaaagaccgc ttgaaagcgg
    13741 tctaaattta ttttttcttg gttgcagaaa tggattttgc aatggtaaag gcagtttccc
    13801 aagcctgttg attatctgta acctcaattt tacggcaagc aatcgcctcg ttgttaaagt
    13861 cagtcatatc aaccttgtcc gaattaacaa gcaaaataac acggcgtaaa gcacaagcag
    13921 caataggatt gtgatcgtgc ccaaacgaac cagtttccat tccgtaagct acatttctta
    13981 actgttgata atcaccactt acaagagcat ttgctgtttc ttgttcagct ttttctgtca
    14041 ttgctattgc tggtaacgac aataagcata aggttgttaa tagcagttct ttcattttaa
    14101 atacctttaa aagttgatta aaaaacacct aaaatctacc acaaaaaagc ccactattta
    14161 aagtgggcta tgcaataaat ttattattta acactcattc cgtgcctatg gttataactc
    14221 gccaatatct gcactaattt atgcaactga tcatctgtca gccattgcac ccgctcaata
    14281 ccaaacgagc gtttggcaat ggcgtgggcg taatcccacg gcttttggct ttgatacagc
    14341 aacgccccga ttttatttat caacccttgc cggtgagggg cggttttact tgctgttggg
    14401 cgtttaccgt attttttcga cttcaccaca aaccctttag ctttcattgt ttgcagtacg
    14461 gtcattaatt cgctatcggt catcattgag cagcttggtt tatccaccgc ctctaacaac
    14521 agcattttat aggcgttatc atccagtttt agctcatttt tgccgatatg gattttctgt
    14581 atcatctgct ttctagtttg cggttgcatt ttgcttttcc tcctgccatt tcagccaaat
    14641 ttgataactt tcggtgttct taacggcttc cagttgcccc atttgcttca ttcgttccac
    14701 atacatcacg gcatcgtgtt tcttttggtc tgcttgccgt tgctgttgct caaggctact
    14761 cactgctttc gcttcacttc ttactactgc aaattgtggc ttcacgcttt catatacttt
    14821 cttcaagtaa ttatgatttg ccagcggctc acatctgcca gtttcacgcc gattccgtct
    14881 tacttgttcc accgtatcca ttaaggcttt ttctaacaca ttagagggag ggtaaagctc
    14941 caataaactc tgcacaattt tcaaggctcg gctattggca aggctgtttt tctgtggctt
    15001 aaataaccct aaatacgcca ccaccgcacg cccacaattc ccaccgatat tgactatcgt
    15061 atttaacaac tcacgccctg catcatcttc aatcaacgcc tcaaacgaga tgtcggaatg
    15121 gcaaatcggg catttgcata gtttcatcat tctgttcctt cttctattgt ttcgagatca
    15181 acccaataag ttcgactatc agggtatggg ttatgccaag ttgctggagt tagctcgcaa
    15241 ataactaatt gttgtaacac aatctctccc gatttctttt tacataaccg atattcagga
    15301 attttttgat attccatttt atgctcctat aaaacacatt attcagccca cttaaatagg
    15361 ggttaaatgg gctgtaaatg ggttttatta cttcccctgt attgctaaaa actcactttc
    15421 tttaatctca accaatccct ctgcaatctc aggaaatggt tcatcatttt tatcaatagg
    15481 cattggcatt tcaaccagta aggtgttttc acaaatgcct gctacggcta aataacttct
    15541 acctgatgtt ggggaataac taaaatcact tacaaaataa agcatattca aacgttttaa
    15601 gatatagggc gaaaaatcac gatgtttctc ataaatttca ttaattgctt taaaatcttc
    15661 tgcaagttct ttcccttttt cgtaacgttt atcaggtttg acgaggtaaa actcatcatt
    15721 aaatttaccg accttgaacc ctttaacatc taaaacttgg ctaatattgg ttgctttaca
    15781 agcaatccct ataactattc tatgaaacca gcctgaccta ctaattaatc catcattgaa
    15841 aggatatttt tctaaaatag cattgagctt atccttgcgt tcttctttag atacttgata
    15901 atctgcataa atgcttttta gtggctcttg ggttatatcc cctttaaaaa atctaaattt
    15961 cattcttaac tccttactgg ggtggttaat gtttccgatt tgccgttaat aacttcaaat
    16021 gcacctttgg caacaaatcc tgcaccgtgc cgagataaac accactgcga ataaagtcac
    16081 tctgttttaa atatttttgt gcctgaataa tctgcattaa cgcctcgttt aattggctgt
    16141 taagttcgtt tttctcttgt tcggtcatca tctcctccta aaacggtttc ttatgcatac
    16201 gtttacaaaa taccgctcgt tgccccgccc agtctcagtt agcggtcttt tttgctgata
    16261 attttgcaac tttccaatgg tcttgggctt ctgcataatt gcctttacgt tcggcttccg
    16321 ctgctttctc ggcataatgt ttgtagcggt caaactcggt cgccttttgt tctgttttca
    16381 ttttttcctc ctgtggttaa aacactttat aaatgccctt ctatcttgtc cccctctttg
    16441 aaaaagaggg gctaggggag atttttaaag ggcattgaaa aggtttttta actaagactt
//
LOCUS       DANLXQ010000042        16430 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.042, whole genome shotgun sequence.
ACCESSION   DANLXQ010000042 DANLXQ010000000
VERSION     DANLXQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.042"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..583)
                     /locus_tag="PXD94_001031"
     CDS             complement(<1..583)
                     /locus_tag="PXD94_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6285910.1"
                     /translation="MFTEGKIDFDKFQQLFSDNFSTEPDRYMLNWAGKSTAYQTLQTP
                     TSKTLTPCEEESVDFENTQNIFIEGENLDVLKALQKSYFNSVKMIYIDPPYNTGNDFI
                     YNDSFADTKADYAEKVGDVDEHGKLKRAFVRNSKENGHYHSNWLNMMLPRLHLAKNLL
                     RDDGVIFISIDDNEQAQLKLLCDEVFGEENFVAI"
     gene            complement(940..1815)
                     /gene="ubiA"
                     /locus_tag="PXD94_001032"
     CDS             complement(940..1815)
                     /gene="ubiA"
                     /locus_tag="PXD94_001032"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL6285911.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
     gene            complement(1808..2569)
                     /locus_tag="PXD94_001033"
     CDS             complement(1808..2569)
                     /locus_tag="PXD94_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL6285912.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGGGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            complement(2636..4003)
                     /gene="purB"
                     /locus_tag="PXD94_001034"
     CDS             complement(2636..4003)
                     /gene="purB"
                     /locus_tag="PXD94_001034"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL6285913.1"
                     /translation="MELTALTALSPIDGRYQDKVASLRPIFSEFGLLKFRVTVEVRWL
                     QKLASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNHDVKAVEYFLKE
                     KCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKVIDAVAELAKC
                     YQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKINGAVGNYNAHL
                     SAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFNTILIDFDRDMW
                     GYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAHLGQKLPISRWQ
                     RDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEMTPASYIGYAVE
                     LVDKL"
     gene            complement(4037..4666)
                     /gene="hflD"
                     /locus_tag="PXD94_001035"
     CDS             complement(4037..4666)
                     /gene="hflD"
                     /locus_tag="PXD94_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL6285914.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNVMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(4798..6462)
                     /gene="cydC"
                     /locus_tag="PXD94_001036"
     CDS             complement(4798..6462)
                     /gene="cydC"
                     /locus_tag="PXD94_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL6285915.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEQQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRCENVSFAYYGEQNVLNNLSFKVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     AVRKVGLGHLLETDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQILNLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVG"
     gene            complement(6462..8228)
                     /gene="cydD"
                     /locus_tag="PXD94_001037"
     CDS             complement(6462..8228)
                     /gene="cydD"
                     /locus_tag="PXD94_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL6285916.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMVAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQENVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELIHNELVHQGEI"
     gene            complement(8343..12440)
                     /locus_tag="PXD94_001038"
     CDS             complement(8343..12440)
                     /locus_tag="PXD94_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL6285917.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVEKTSEKVVEGFHQLQRKTVAQAEQVDNKV
                     DWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLNV
                     ASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVKL
                     PFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPFA
                     PQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQDL
                     SLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGRG
                     NSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGVL
                     GEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKLQ
                     NLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVGNATLLLKGNEENHSLKLSAKGNPVG
                     ADLQISGKFDRLKEIWEGQLSQVSIQSTEFGQFQADKAVNVKYDNKAINANISAHCWH
                     NRKINLCFPTAFNAGVEGKVPFEIRNFDLAVLQQFLEKNSQLSGIVNAKGDAAWFKNK
                     QPQVNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKSDLRVENNGRLS
                     TDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLTVSGTATSPLLH
                     GNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELRLEGDADWRDLN
                     AWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIPWARIEVEQLPE
                     SAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGNDVKLKAYGLNS
                     NVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGSPSQPTLDIEAI
                     RNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGRSLESSGDTSSS
                     NAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVSASLAPKFRVKY
                     GVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
     gene            complement(12521..14356)
                     /locus_tag="PXD94_001039"
     CDS             complement(12521..14356)
                     /locus_tag="PXD94_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL6285918.1"
                     /translation="MNTKLHFLTFCCLVAFASMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQGKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     SHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            complement(14368..15213)
                     /locus_tag="PXD94_001040"
     CDS             complement(14368..15213)
                     /locus_tag="PXD94_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDL6285919.1"
                     /translation="MFKQINKRLNFNIFLQMFVLFTPLVQAAQMAIVIDDVGYRIKED
                     REILALPKAVSVAIIPVAPYATERAKDAYNQKRDILIHLPMEPKSKQPIEEGGIHIGD
                     NEEKIRKLIHTSRGQVPYAIGLNNHMGSGATSDSATMQHLLKVLKENTLFFLDSKTIG
                     SSVAAKTARQFGINTLERDIFLDDSDLLADVQKQFAHAINHARKNGVAVVIGHPRKNT
                     ISVLKQNLAQLPQDIELVSVGNLWRNEKTVPDKPFIMLFEQEPALSSVPPYDSIPLLR
                     GIPKE"
     gene            complement(15214..16377)
                     /locus_tag="PXD94_001041"
     CDS             complement(15214..16377)
                     /locus_tag="PXD94_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL6285920.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QADLKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGTPINPLNLVK"
BASE COUNT         4573 a         3666 c         3101 g         5090 t
ORIGIN      
        1 atatggcaac aaaattctcc tccccaaaca cttcatcaca aagcaatttc agctgtgcct
       61 gttcgttatc atcaattgaa ataaaaatca ccccgtcatc acgcagtaaa ttttttgcga
      121 gatgcaaacg aggcaacatc atattcagcc agttactgtg ataatgccca ttttctttgc
      181 tgttacgaac aaaggctcgt ttcaatttgc cgtgttcatc aacgtcgccc actttctccg
      241 cataatctgc tttggtatcg gcaaaactgt cgttataaat aaaatcgttg cctgtattgt
      301 atggcggatc gatataaatc attttgaccg aattgaagta ggatttttgc agggctttta
      361 atacatctaa attttcaccc tcaataaaga tattctgcgt attttcaaaa tccacactct
      421 cttcctcgca aggagtgagc gttttgctgg ttggggtttg taaggtttga taggctgttg
      481 attttcctgc ccagttgagc atatagcggt caggttcggt ggaaaaatta tccgaaaaaa
      541 gttgttggaa tttgtcgaag tcgattttac cttcggtgaa aatttcaggt aaaagggatt
      601 ttaattgaga aagttgttga tctttaagcg aaaagttggc aaggctgtgc tgtgctgtgc
      661 tgtgctgtgc tgtgctgtgc tgtgctgtgc tgtgctgtgc tgtgctgtgc tgtgctgtgc
      721 tgtgctgtgc tgtttggcat tgtaatgtcc cttttttgaa tgtctactac aaaatgggat
      781 attttacaaa ccgattgact aatgaagcaa gaatataatt aactacaact ggatatattt
      841 tgcaaatttc ccgataaatt tcaccgcttg tagataaaaa ccgtagataa aaaccataga
      901 taaaaaatag gagctaaaca tttttagccc ctaccgtcat cagaaataaa tcccgactaa
      961 aatcgccacg aacacaccta agccgaaata gtggttattt agaaacgctt taaagcattc
     1021 gctacgttct ctttttttgg tcagccaaca ttggtagata aacagcgtgg cacttaaggc
     1081 taatacgaca aaatagccga tgtgataatc ttttaccacg cccaataacc ccaataaaat
     1141 caaggtgata aactgtaata acgcaataat tttattatcg tattgagcga ataaaattgc
     1201 agtggattta actccaattc gtaaatcatc gtcacgatca accattgcat attgggtgtc
     1261 gtaggcgact gtccacgcta gattggcaaa aaacagcagc cagcattcaa gtggaagagt
     1321 ttccgttacc gcaccaaatg ccatcggaat cgaccaaccg aatgccattc caagcactac
     1381 ttgtggcaag tgggtaaaac gcttcataaa gggataaatc accgccagaa aaactgccac
     1441 aaaggaaagc agaaacgcat agcggtttag cattaaatcg agcagaaaag cgaacaacag
     1501 caagatggca aaaatgactt ttgcctcagt ggtggtcact ctgccggtag ctaatgggcg
     1561 ttgagaggtg cgtttaacgt gtccgtcaat ttctctatcg gcataatcat taatcacaca
     1621 acctgccgca cgcataacga ttaccccaag ggtaaaaatg gctaaaatcg caacaggcgg
     1681 cattccctct gcggcaataa ataacgccca tagtgtgggg tgtaataata ataacgtgcc
     1741 gatgggttta tcgaaacgca ttaactgggc gtaggcaatc catttttgtt tagaaaaaag
     1801 agtttcgtta ttcattacaa attttgacga taacgtccga ttggcttaaa tgagtctgta
     1861 attctaacgg taaatcttga tctgtaaaca aataatttac ttctttaaca tttcctaaac
     1921 gtgcaatagc tgtacgggta aactttgaat gatcggtcac taaaaccact tgtcgggaac
     1981 attccattaa tgcacgcttt acctgcactt cgtggtaatc gtaatcgagc atagagccgt
     2041 ccatatccac cgcactaatg cctaaaatcc caaagtccaa acggaattgg ttaataaagc
     2101 gaaccgtttc ttctccgatt agcccgccat cacttcttag ccctccgcct gctaccgtaa
     2161 tttggcaatc ttcattttgc atcagaatat gagccgcatt taaattattg gtaactactt
     2221 ttaaattttt atgcgaaagt aaggctaatg ccactgcttc tgaagtcgtg ccaatatcta
     2281 aaaataatga tgctccattt ggaatcagtt ttgccacctg ttgggcaatc gcatttttct
     2341 cgtgtgagaa aaattgcttg cggtgggtgt aatcactatt ctccgtattg gaaggagcgg
     2401 ctgccccacc gtgatgacgg cgaatcagat tattttctgc catttcattc agatcacggc
     2461 gaatggtttg cggactcaca ttcaattcag ccactaattg ctcggtactc acatagccaa
     2521 gctgattaac cagctcaatg atctttttgt gtctgtttgc ctgtttcata tttcctcttt
     2581 tgcaaataat ttgcagaaaa agaccgcttg caagcggtct tttttcagtt aaattttaca
     2641 atttatcaac aagttcaacc gcatagccga tatagcttgc cggtgtcatc tctttcaaac
     2701 gatttttttc attttccgga atttccaact tatcgataaa ctcacgcatt gcggtttcat
     2761 caacccgttt gcctcgagtc agctctttaa gtttttcata cggtttttca atgccataac
     2821 gacgcataac agtttgaatt ggctctgcca atacttccca gttttggttg agttcatcac
     2881 gcaagtgttg ttcattcact tctaatttgc taataccttt taaggtggac gcataagcaa
     2941 ttaacgcata acctaaacct acacctaaat tacgcaacac ggtggagtca gttaagtcac
     3001 gctgccagcg agaaatcggc aatttttgcc ccaaatgagc cattaccgca tttgccaagc
     3061 caagattgcc ttcggaattt tcaaaatcaa tcgggttcac tttatgtggc attgtgctag
     3121 agccgatttc accggcaata gtgcgttgtt tgaagtgatt taaggcaatg tagccccaca
     3181 tatcacgatc aaaatcaatt aaaatcgtat taaaacgagc cacgcaatca aagaactccg
     3241 caatataatc gtgcggttca atttgggtgg tgtatgggtt ccacgtcaca cctaacgatt
     3301 ccacaaattc ttggctgaaa gtgtgccaat caatctccgg atatgctgat aaatgtgcgt
     3361 tatagttgcc cactgctcca ttgattttag cgagaatttc taaattttct aactgtttat
     3421 attggcgttg taaacgatat gccacatttg ccatctcttt acccattgtg gtcgggcttg
     3481 ccggctgacc gtgtgtgcga gaaagcaacg gaatatgttg gtaacatttc gccagctctg
     3541 ctacagcatc aatcactttt ttccattcag gtaataatac ttcttcacga gcggttttaa
     3601 gcattaaagc gtgcgaagta ttattgatat cttcagaagt acaagcaaaa tgaataaatt
     3661 cattcaccgc ttgtaattca ggcaacgctt cacatttctc ttttaggaaa tactccaccg
     3721 cttttacatc gtggttggtg gtacgttcaa tcgttttaat ccgattggca tcttctaacg
     3781 aaaaatcagc cacaatttgg tttaggtaat cgtttgcttt ttcagaaaac actggaactt
     3841 cattaatttg tgtatgcgat gccaattttt gtaaccagcg aacttccacc gtcacacgga
     3901 attttaataa gccaaattca ctaaaaatcg gacgaaggct cgcaactttg tcttgataac
     3961 gtccatcaat cggggataac gcagttaaag cggtaagttc cattgtttta tattctccaa
     4021 ataggtaaat gttcggttag atttgacgat aaaatttctg tgcttggtta aaaattttct
     4081 tgcgtgaaaa taaaaattgc caacggcttc cgccgacttg ctgccacaaa ataccggctc
     4141 gaataccggc tagtagcgag gctcgaattt tattctgtat atccgggcga accaagtaat
     4201 cttgcattcc catcacgtga attttcgtac ctaacgggct gataatatcg ctataaatag
     4261 cggctaaatt ggcaatcatt tgatctgcca tcacattatc ttcatacagg gctaactggc
     4321 gttccacttg ctgtaaacgc tgggctaact gttgttttgc ttcagggttt ttattgagtt
     4381 tttggctaag ggcgagcagg cttatccaat aacgtccgac ttcggtatct aatttgccgt
     4441 taccgccgcc agtttgagct aatgcggttt ctaagcccat ttttaaatgg gaaagatcat
     4501 cgccaaatac acttaacgtt gattccggct gagtgattaa caggctttct aaagcacact
     4561 taaaagcctc tctgtctgcc atgcctttat gtgcaaattg ttgcactaac gtcgccgctt
     4621 ggcaaacgcc ggcataagca agggtaatat cgtggtaatc cgccatctct gtaaaattca
     4681 atatgataaa acattagtgg gaatatagca ttttttcagc ttcttttgaa tggcaaaagg
     4741 catttattct attgaaaaat aaatgccttt tcattaatac gcaatttgct gcaaaaacta
     4801 tcctaccaat cgcccattat ctattcgata aaccacatca aatttatcca gtccgctttg
     4861 gcggtgagtg atcataatta aggtgcgatc acagcagtga gtcaaaatta ggtttaaaat
     4921 ctgctgttcg gtttctctgt ccagtccttc tgttggctca tcaagtaaga ctaattcagc
     4981 agaactcagg agcaaacggg caagccctaa acggcgttgt tcgccaccag aaagcggtct
     5041 gccgccctcg cctaaccata aatttaagcc gtctgtttca agcaaatgcc ctaagcctac
     5101 ttttcttact gcttcttgca tttgttcgtc tgtagcttga gcattcccca ttaataaatt
     5161 atcttttagg gtttggctaa aaatatgcac tcgttggctt aaggtaacta attttgagcg
     5221 taacatcggc tctgaatatt cgtcaatttt acaattattc agccaaattt gaccgcttgt
     5281 agggtcgtag ttgcggttta acagttggaa tatggtcgat tttccgctac ccgttttacc
     5341 taagattgcc actttctgcc cttttaatac cttaaaggaa agattattta gtacgttttg
     5401 ctcgccgtaa taagcaaagc tcacgttttc acagcgtaca agcggttgat tttggctcaa
     5461 attttgccat tctgccttgc taccaaatgt tacattaggc tgttgttcgg taatttcact
     5521 aatccgctcg gcggcggtaa tcacttgccc taaatgcaaa aaggcgatac caatcgggga
     5581 aagaatctcc gccgatgcca ttacacagaa aatcactaac gcaattaagg cttcagggta
     5641 ttttgcagtg ggcacattaa tcgaagttga aaccaaatag atcaccacac aagtcaaaat
     5701 gccgttggat aacattatca agctatttga taagccggac agtcggcttt ctttgctttg
     5761 agcgttcagc cattgttgtt cagtttgctg tagtttttcg ctcatttggt tcaagttgcc
     5821 aaagagtaaa aattctgcat ttaactgtac ccactcaata aattggctgc ggtaatttgc
     5881 tcggctttgt attgcattac gccctaattt caaacccaat cgataaaata cggtaggaat
     5941 aacggcaagc aataccaata gtgtgccgca aatgatcagc attaatggca cggaaacaaa
     6001 agaaagcccg attgccataa atgcaataat catcactgca ctgacaaaag gcgagactaa
     6061 attcagatat agggtgtcta acgtatccac atctgccacc aagcggttta acaattcgct
     6121 gtttctaaag cgatttagcc cgcttggact cagtggaatc aattttttaa agacgctagc
     6181 acgcaaattc gccaataccc gaaaagtcgc atcgtgggtg actaaacgtt caaagtaacg
     6241 tgaaattgtc cgccctactg ccaaccctct cacccccgat gaagggtaga aaaagttaaa
     6301 aataagcgca ttgccggcta gaaatgaggc agctaaaaac caaccggaaa ggctgagcaa
     6361 accgatactt gccgctaatc ctaaaattgc cagcacaaca cctaataata gtcgcccaaa
     6421 atgggtttgg tagagagcaa aaaacgggaa caacgatttc attagatttc tccctgatgc
     6481 actaattcat tatgtattaa ttccgcaaaa aagccggtgt gttctaattc agtgaaacga
     6541 cctttctgaa tgatttgccc ttgtttcatc acccaaattt catcgcattg ggttaaatct
     6601 tccactcgat gagtaatcat tagcgttgtt tgctctcggc tgagattatg cagagcggct
     6661 aacacttgct gttccgaatc catatcaagg cttgcagtcg gctcgtctaa cagtaataat
     6721 tcgtacgggc gaagcaacgc tcgtgcaatc gcaatccgct gtgcttgccc gccagagata
     6781 ccgatattac tgtcttgcac ttgatgctcc aaccctaaac gtgccacaaa ttcatcggct
     6841 tttgaaagct gtaatgcctt ggctaattca gaatctgtcg cattagggtt gcctattaaa
     6901 atattctctt tcaaactgcc atgcattaat tgcggatttt gccccaccca cgctaatttc
     6961 gctcgccatt ggcttaaatt taaatctcgc agttcggtgc cgttaatcgt aaccgaccct
     7021 tcataaggca agaaaccaag taacatattc attaatgaac tttttcctgc accactttgc
     7081 ccaaccaaag cgatatgttg gtgtgcattc aactcgaaat ttaacgcttg ggttaaggct
     7141 ttgccttgtg gtgagaagat cacgcaatct ttggcttgaa tcacaagcgg ttgattttca
     7201 acattttctt gcaaattgcc accttgcgtt ttcacctttt cttttaagaa agtttcgata
     7261 ttatctgcgg cactaatcgc tgctgccttg tcgtgataaa acacgccaag ttcacgcatc
     7321 ggttggtaaa actcaggtgc caccattaga caaaagaagc cgataaataa ggtcacgccc
     7381 gtaccataat agccaaaatc cagctcacct aaaaaaataa agccaaaata gaccgccgtc
     7441 accgcaatcg ataccgccgt gaaaaactcc aataccgcag aggataaaaa cgccattttt
     7501 agtacgtcca tcgtactgat tcggaaatct tcggtgcttt ggtagatttg ttcagtctgc
     7561 ttttccgctt gcccaaataa gcgaatggtt tctaaacctt ttaatttatc taagaactgt
     7621 ccgctaatac gggaaagaat gccgatatta cgctgatttg cctccactgc tttcattccg
     7681 gctaaaatca taaataaagg taataatggc attgttgcaa ataaaatagc ccctgctgcc
     7741 cagttaatcg ggaatacaaa acagaggatc agtaatggca ctatcagcga taaaaattgc
     7801 tgtggcaaat agcgtgcgta gaaattatgt agattttcca cctgctcgag cattaaggtt
     7861 gcccagctgc ccgccggttt ttgttgaatg ctcatcggcc cgactgcttc cattttggct
     7921 agaatttggc ggcgaagatg taaacgcaag gtttgccctg ctttgaaacc gacacgctca
     7981 cgcagaaaaa tgaagaatgc tctggcagca aaacaacaga ataagagcat aatttcaacc
     8041 agaaagctat tcggtgactg ctttccgata agcattttgt ctaacattgt ggcaatcagc
     8101 cacatctgtc caatcataca taaactactg aaactcccta acagtatgtt taagtgcata
     8161 tattttttga tgactttttg ctgactttta agccattttt gtaattcgcg ttggcgttgt
     8221 ttttccataa ttttgtcaat ttttttgttt tttcaaccgc ttgtaaatcg ggtttgccat
     8281 tctactataa agcaaaaagg ttgttaatgg cgaaacatta acaacctttc attcagcctt
     8341 aactagaata gctcgtcaaa ttcaaagcga tacattaaat ctaccgcttg gttaatactt
     8401 gagacccatt gtaagtacaa tcttggcgtt aaattatagc gtaaggttaa ttcggtaagc
     8461 ggtgcaaaaa tgcccacgcc atacttcact ctgaatttag gggcaaggct ggcacttact
     8521 tctactttgg tgttatcgcc aatacctgcg gtggtcacgc ttaaatcttt gagtccgaaa
     8581 gtgctaccaa catcacccac caatttactg ctttttgata agctcatact gagtaatgcc
     8641 gctgcgactg cattactgga gctggtatcg ccactgcttt ccaatgaacg accagttaaa
     8701 atataggaaa gtgcttcatt ttgcgacatt gccggatcag agaatacttt taccgttgga
     8761 ttgtctgcca aaccggtcac tcttacccca gctgtaatgg tcggatcttc cattgcctcc
     8821 ggattgcgta tagcttcaat atctaaggtt ggctgagacg gtgagccggc aaaactaata
     8881 tcacctttac gaatcagcaa gtcttgacca taagcggcaa atcgtccttc acgcaaatga
     8941 acttgcccgt aaagccctaa accttgtttg ccttgcctga cagacaataa gccattcacg
     9001 ttactgttta aaccataggc tttgagtttg acatcattac cgatgttaat cttaatatct
     9061 gcattaattg ccattccacc tgaggtttga gccggaatat tacgttggct aaacggcact
     9121 ttatttttca cagagccgtc cataatcacc tcatctccac ttacactgac cgcactttca
     9181 ggtaactgct cgacttcaat tcttgcccaa gggatatcta catttccact taaggttaaa
     9241 agagttggcg ttgccgtcac ttgaatatcc gggctaactg ccacttttgc gatattagga
     9301 atatccactt ggaaacggtt agcttgagcg tgaacccgtg ttttccacgc atttagatct
     9361 cgccagtctg catcaccctc caaacgcagt tcgctttgtt tggtttgtaa tttaccgctt
     9421 aaagtcgatg ttgccccgtg gaaattcatt gctaaattcc cacctgtaat atcaaacggc
     9481 attgtaacag agcgagcttt taactctgta acgtttagat ttccgtgcaa taacggagag
     9541 gttgcggtgc cgctcacggt aagacgagca ttcacattgc catcaaccga atcgccccgt
     9601 tcaagtagcg gtcgaatcag ctttaagctt aattggtcaa gatgaatatt gccggataac
     9661 gcacgagcct tacttaaatc tttcatcact aaatcagtcg aaagtctgcc gttattttcg
     9721 acccttaaat cagatttaag ggttaaatta ttgtcagaca ttttcagatc aattttaagc
     9781 ggtgaaacgg tgagtgggaa actttttccc ccctccattt tttgaataaa tttaatcgca
     9841 ttagaggtta aatccagatt cacttgaggc tgtttgtttt taaaccaagc tgcatcgcct
     9901 ttggcattca caatccctga aagctggctg tttttctcca aaaattgttg caataccgct
     9961 aaatcaaagt tacggatttc aaaaggcact ttaccctcaa caccggcatt aaatgcagtc
    10021 gggaaacaaa gattaatttt ccggttatgc caacaatgtg cggaaatatt ggcgttaatg
    10081 gctttattgt catatttcac attcactgct ttatctgctt ggaattgacc aaactcggtg
    10141 gattgaatcg aaacttggct taattgccct tcccaaattt ctttcaaacg gtcaaatttt
    10201 ccagaaattt gcaaatcagc accaaccgga ttgccttttg cacttaattt caagctatga
    10261 ttttcttcat ttccttttag caataaagtg gcatttccca ctttgatttc attataggca
    10321 aattgacgta accccaaggt taaatcccct tgaatggttt tctcagtggt aattttgcct
    10381 tttgcggtta aattttgcag tttaaattgc tcatagccta catttgaggc cactaaatct
    10441 aaatcaatgt taggttctga aaccttaccc gataatttgg cattaccatt cacgctggct
    10501 tttaaattcg gcactaaccc ttgcaaatta ggggctttaa tatccgcata gaaatcagat
    10561 ttttcgccta acacgccttt taaatcgatc ttattttcac catacaccag cgataagccc
    10621 ggaacatcta aaagttgttg atgatcggct tttaaattac cgcttaagtg tagatttttc
    10681 tgtaataacg aacctttaat atcaatattc gagacgtccg ctttccatcc gctagctgaa
    10741 ttgccccgtc ctgcataacc ttcggaagat aaagaaccgg acaataccgc cggccagtct
    10801 gccagtaggg atttagtgtt aatatgctga agtttcaaat ttgaacgcca cgcaacgccc
    10861 tcgctccaat ccactaaacc gtcgatcttc gccgtacctt ccagcgtatt gagacttaaa
    10921 tcttgtatat taaaatgggt taattcccct ttgccagtta aactagccga gcttgcaggc
    10981 acgcccattc ctttggcact gactttagcc tccaaagcgt aatccaggag attgccatta
    11041 agcctcatgg cgatattctc taaactcagc acatcaccat tttgctgagg agcaaacgga
    11101 taacgcattg actcactctt cagcgataaa tcaaagggag ttttcgcctg agccagtgca
    11161 acattgccct ccaacgaggc gctaattgca ccagccgtct tcacttttag gctggttttg
    11221 tcatatagct caccggaaag agaggcttca atttgattga acggcaattt tactttcgcc
    11281 gtttttgcct cgtgaccact tagcgtccaa cttagcggat aattatcttg taacgataaa
    11341 gaaccgcttg cggttagatc cccttggtcg cttttgaaat ctaaactttt taggttgata
    11401 tgctgatctt ttgcttgagc ctgaagctgt aaagaggcaa catttaacaa tgagatcggc
    11461 tcaatcaaat tccctttttc atctttagct ttttgagcaa tcgctacatt cgcaatatca
    11521 atctgttcaa tattgagatc aagaggcaaa ctaaagcgaa tatttttatc caataacggc
    11581 tgtgctaatt gtgctttaat cgctgcccaa tccactttat tatccacctg ttcggcctgt
    11641 gcaacagttt ttctctgtaa ttggtgaaag ccctctacga ctttttcact ggttttttcc
    11701 accgcctctt gaatcacttt atcagcaatc tcttgtggat ttgtcgctga taacgcctct
    11761 gtttttgcat ttttttgtgc aaaatcgacc gcttgttcag tatcagtttc gtctaacttt
    11821 aaggctaaaa gcagatttag cccctctaac tgtgtcgggt gaacggtaat agcccgccct
    11881 ttaccagaaa gtgcagtgtg gaaatgatta agatgaatat ccatatcatc aactttgacc
    11941 ttgatattct ctaaattgat attttgagct gaaatcccca atggcaaatt taattcggtg
    12001 aaaggttcgg tctcttcttt tggctgagaa gggggtaatt tagtcgtatc aatcaacacc
    12061 tctgtatctt taaggctgag attattcaaa cacatttcat agtggagcaa gcagctaaaa
    12121 ccaatatgca gatctgcttg cccaaccgtt acattcacac cgtccatcac aaatcgggca
    12181 tcagaaagct gtaacccatc ttgcaggcta ccttgcactt gcccaagttg taatggctct
    12241 acgaatttat ctacccagct taacacttta cgttgcccgt aacctgtgca tagaaagaga
    12301 atggcgagta aaagtagaat aaacgtcaga caaaagccgt gcttcaaaaa ccgccacttt
    12361 gattttttag gctgtgttgc ttgtggctct tgtgctgagt ccgtttgatt tgtggctgtt
    12421 tgtttatttt cgtcagtcat aatcgtgcat atttatgagg tgataaaaag taaacaagcg
    12481 gtatgcgaaa ttttactgaa attcgaccgc ttgttttcat ttacagatcg gaacctaaac
    12541 caatataaat ttgtattcct ttggaattat ctggtgattt taccggcgtg gcgacatcaa
    12601 atttaatcgc cccgataggt gatgcccaac gcacccctaa gcccgtaccg tagtagagat
    12661 cctgagtttt gaatttattc gatgccaagc ctgcatcata aaaaattgca ccccaccaat
    12721 tagggtaaac ctgatactga tattctactg tgcctgtggc taaatgagag cctccgacac
    12781 gtttgccatt actcggatca cggggggaaa tatccttata gccaaagccc cgcacactca
    12841 tatcgccacc ggcaaaatag cgtaacgccg gtggaatacg ttcaaattga ttggctttca
    12901 aataccccaa ctccgccctt aaatagaacc gatggttatc aaagtaagtt ctcgcccaag
    12961 atgtcgaggc tttataacta taaaagttta catctgaacc tactgcacga ctcccccagt
    13021 ttaccgttaa tttttgtgta tccccccata atgggtaagg cttgccatct gttctggttc
    13081 gattcaatga tgccgttggg taaactaata aggttttaaa tcgatcatta gcttgagtaa
    13141 aagaatcgta acgggctttt aaaccaacag aaaatgacca accggtttcg tgagtccaga
    13201 aacgttgtaa gccaagggtg gcagcggttg attttgtatc gttttgatct tcgttttcta
    13261 aagtgccgga taattggtaa taataatgta gcggatcttc tctaagtgga atattataac
    13321 caaattcaaa tgtctgctcc ggcttagaaa cgtacgttcg gctttcaata ctatgacctc
    13381 ggctatttaa ccaaggtttc ttccagttaa gctgtaacct tgggccaact tcagtttcgt
    13441 agcctatccc aatttccact tcattgcgtt ttttaggctg aaataacaca tttaaatcca
    13501 ctaatttatc ctgttcattt aaatgtgact caaccatcac tgatgaaaac caattactgg
    13561 aaaggtaatc ttgggttaaa atcgaaagat cgttagctaa atatggctca ccggaatgaa
    13621 ttttaagaat attttgcaaa tattcttctt tgatttggct atcaacaaac cggatttgtc
    13681 cataatggta tcgttttccg ctatcatagc ctaaacgcca atccgcttta tgatcggtcg
    13741 gataaacttc caaacggtga taaagccaat gaccgtcaaa atacccttta ctaaaagcgg
    13801 tactttcaat atcaccttta aaattatcat aggttttatg gtttaaacga ctaccttgtt
    13861 ttggggcttt actgactaga tgagcgaaat cttcgtccgt ttttgcatcg ccttgcaaaa
    13921 caatatcggt ttcatcaatt tttaccgtct ctttatctag ctcaacattt aatactaaga
    13981 gcggtttctt tccctcttga ggtagctgca caaattggta tcggctatta taatagccca
    14041 aggctctcag agctttatct actgtggttt gcaccaaata ttgataacgc tctgaaccat
    14101 ctgcatattc attggcaatt tgatctaaat atagctccac atttttcttg gcatctttac
    14161 cctgaatgcc tttgacttct aaagtgacta atggctctat ttctggctca tcatcactat
    14221 cttccgctaa tttttcttgc agattttcaa ctcgctcacg atctacgtca gacagccttt
    14281 gctcttgatt tgcttgtgcc attgaagcaa aagccactaa acagcagaat gttaaaaaat
    14341 gtagttttgt attcattagt aaccttttta ttcttttgga atgccacgca aaagcggaat
    14401 actgtcataa ggcggaacag aacttaacgc tggctcttgc tcaaagagca taatgaatgg
    14461 tttgtcaggc actgtttttt cattacgcca caagtttcca acactcacca attcaatatc
    14521 ttgaggtagt tgtgctaaat tttgcttcag tacactaatc gtattcttgc gagggtgtcc
    14581 tatcaccacc gctaccccgt tttttcgtgc gtgattaatt gcatgtgcaa actgtttttg
    14641 tacatcagca agcagatcgc tatcatctaa aaaaatgtct ctctccagcg tattaatacc
    14701 aaattgacga gccgttttag ccgccacact actgccaata gttttactgt ctaaaaagaa
    14761 aagcgtattt tcttttaaca ctttcaacaa atgttgcatt gtagcagagt ctgaagttgc
    14821 tccactgccc atatggttat ttaaaccaat agcatatggt acttgaccac gagaagtatg
    14881 aataagtttc cgaattttct cttcgttatc accaatatga atacccccct cttcaattgg
    14941 ctgtttcgat ttaggttcca ttggcaaatg aatcaaaata tcccgttttt ggttataagc
    15001 gtcctttgcc cgttctgttg catagggtgc aacaggaata atcgccacgc ttaccgcctt
    15061 gggtaaggct aagatttctc gatcttcttt tattcgatag cccacatcat caattacaat
    15121 cgccatttga gctgcttgta caagcggtgt aaaaagcaca aacatttgca aaaaaatgtt
    15181 aaaattcaac cgcttgttaa tttgtttaaa catctatttc accaaattta gaggattaat
    15241 cggcgtgcct ttacgggtaa caccaaaata gagtgcagaa cggctttgtc ccccactgtt
    15301 accaaccttc gcaataacct gtccgccttg cactcggcta cccttgctga ctgaaataga
    15361 ttggttatag ccataaaggg aaatatcacc attgccgtgg tcaatcgcaa ccatattgcc
    15421 ataccctgca agccattgag ccattatgac tcgcccgctc gccattgctc taaccgaagt
    15481 accggcagaa gcaccaatca ctacaccgtg ccaattatta ccaaaaggag tgataatatt
    15541 gccagacacc ggcatcgtat atcgaccagc cttgatcggt gttctctcaa cattctcagt
    15601 tgttttggac tgaccggatt ttgcctgttt ttcccgctgt tgttcacgtt tttctgcttg
    15661 ctgagcctct ctttgcgctt gttctaattg ttgttttagt gcctgttcat tgctgcgtaa
    15721 attctctaat cggctttgat cctgctcaag cgttttatcg attaaacgta aggtactttc
    15781 acgctggctt tgtactttct ttaaatcttt ctcttgtttc ttttgttcat taagttgcgt
    15841 ttgatgatct ttttgctggc ctttcaattc atcacgtctg gctttaagat cagcttgggt
    15901 tttacgaatt tcaacgatcg catcaatgcg aatattattc atatgctgat aataagcggt
    15961 cattctttcc gcattttttg cctcttcaga cattaaacgc tctaagaccg aagggttcac
    16021 cccagaacgg taggcagagt ccaactgctc ttttaatttt tctttctgct gtttttccaa
    16081 cttttctaaa cgggcaattt cagtctcagt cgctttaatg gtctggcgaa cttccgttaa
    16141 agacatttcg gttttttgta atttatccaa caccttcccc atttctactt cttggttttt
    16201 tagggtagat tgcaacgcat tacgtttttg cttttgttca ttaatttttg aggtttgctg
    16261 ttggattttt tgctgaatct gattaagatt atttgcaaaa gaaaaagggg tagaaaagct
    16321 tattacccca ctgaaaatag cagtccaaca gtaagaattt attcgtttca ccagcatacc
    16381 tttgtttctt caaaatatta ttaaggttat attctaatcg gttatttgct
//
LOCUS       DANLXQ010000043        16364 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.043, whole genome shotgun sequence.
ACCESSION   DANLXQ010000043 DANLXQ010000000
VERSION     DANLXQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16364
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.043"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            151..480
                     /locus_tag="PXD94_001042"
     CDS             151..480
                     /locus_tag="PXD94_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285921.1"
                     /translation="MKKYLFFAFMTLSNVAYPNLDEWYSASDKERREFIAKHFAEETV
                     RSDRNTPYDYAMCTGRMFAKQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQ
                     NCAKYIQ"
     gene            complement(516..1268)
                     /locus_tag="PXD94_001043"
     CDS             complement(516..1268)
                     /locus_tag="PXD94_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL6285922.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(1307..2554)
                     /gene="clpX"
                     /locus_tag="PXD94_001044"
     CDS             complement(1307..2554)
                     /gene="clpX"
                     /locus_tag="PXD94_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL6285923.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQATNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(2554..3147)
                     /gene="clpP"
                     /locus_tag="PXD94_001045"
     CDS             complement(2554..3147)
                     /gene="clpP"
                     /locus_tag="PXD94_001045"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL6285924.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTRRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            3259..3642
                     /locus_tag="PXD94_001046"
     CDS             3259..3642
                     /locus_tag="PXD94_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL6285925.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(3746..3822)
                     /locus_tag="PXD94_001047"
     tRNA            complement(3746..3822)
                     /locus_tag="PXD94_001047"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3786..3788),aa:Asp,seq:gtc)
     gene            complement(3911..4666)
                     /locus_tag="PXD94_001048"
     CDS             complement(3911..4666)
                     /locus_tag="PXD94_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6285926.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLAGQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(4726..5721)
                     /gene="trpD"
                     /locus_tag="PXD94_001049"
     CDS             complement(4726..5721)
                     /gene="trpD"
                     /locus_tag="PXD94_001049"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL6285927.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVY
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKVTAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(5739..6125)
                     /locus_tag="PXD94_001050"
     CDS             complement(5739..6125)
                     /locus_tag="PXD94_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL6285928.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQAHR"
     gene            complement(6118..6708)
                     /locus_tag="PXD94_001051"
     CDS             complement(6118..6708)
                     /locus_tag="PXD94_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL6285929.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMLVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(6718..8274)
                     /gene="trpE"
                     /locus_tag="PXD94_001052"
     CDS             complement(6718..8274)
                     /gene="trpE"
                     /locus_tag="PXD94_001052"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL6285930.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVANFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            8416..9033
                     /locus_tag="PXD94_001053"
     CDS             8416..9033
                     /locus_tag="PXD94_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL6285931.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTSFN"
     gene            9179..10246
                     /gene="rluB"
                     /locus_tag="PXD94_001054"
     CDS             9179..10246
                     /gene="rluB"
                     /locus_tag="PXD94_001054"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL6285932.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRSTVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            10312..12084
                     /locus_tag="PXD94_001055"
     CDS             10312..12084
                     /locus_tag="PXD94_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL6285933.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDVVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIAAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(12174..16325)
                     /locus_tag="PXD94_001056"
     CDS             complement(12174..16325)
                     /locus_tag="PXD94_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL6285934.1"
                     /translation="MNKIYRIVWNHAKRTWIVASELSRSANKSNATNTSLTTNIIKLS
                     TLSLALSAGFASAATYSPTYGTIPDNNPQTSIAIGDGSKVNSTLVSTITGLEGNSGKV
                     STALGYNTTATADFSTALGGFANASFASTAVGGQATADKRSVAVGYNATAMGLREVFI
                     GDHAGLNHSNSTEYNIGIGYSASSNVTGNNTISIGNTAGDGTSGSHNIAIGTYANAKL
                     AGPTTNVTSDDNIAIGNSALANGVNNYKTTAATGETVIGKATAVGSHANATGIVSSAY
                     GAEANATAIHSTAIGAKSQANGDNSTAIGYEAKTSGHGATSLGYYANATANLTTAVGT
                     NAGATSDYASAFGREANASGGSATALGNRATASGAASVALGVSAKATNQRTIAIGESS
                     NASAFNATAIGRNATAEHTDSIALGSNSVTAIAIPTTNATVNGITYSDFAGTNPIATV
                     SIGAEGKERTITNVAAGRISLSSTDTINGSQLYLTQQAIGNVAATTANILGGGAAVTE
                     NGNITFPTYALVNGTPDADKEGKQGRYTTVSAALSALNTAVISPLTFAGDTGTNFERH
                     LGSTVKIKGGSTGILTENNIGVVADGNSTLTIKLAEKVNLGANGSLTTGDTVVNNTGI
                     TIANGVADKPVSLTKSGLDNGGNKIANVAAGDVDTDAVNVSQLKQAISKFATHYVSIS
                     DDGIQRANYDNSGSSGVNPMAIGVATSANGELATALGSEAEANGERTTAVGPRATADG
                     MNATSIGYNANANATNALAVGSAANANADTSTAIGTASTATATRATALGSKSEATGEN
                     STAVGYEASSIGADSLAAGYNANASGTQSTALGNSANAGGIWSTSVGRNANAAGSSAI
                     ALGNSANAAGVASIALGVSSQATTTAAVALGQNAKATHQGSVALGTNSETVATVATKS
                     ATLNGNTYTFAGTTPSSTVSIGSVGNERTLTNVAAGRILDSSTDAINGSQLYAAYTEI
                     DGLNTKVNELSNGALTFVDDAGTEIVRKLGTSLNVKGGADATILTDNNIGVVATDANT
                     LTVKLAKDIDLTPAGSVAVGNSKLNNNGLTINNGPSVTMTGVDAGKLKITNVADGDIS
                     PISADAVNGSQLYDTANTIATALGGNSSVNANGAVSAPSYTVVDGAPTNEVSKTVNNV
                     GSAITALNDAVTSPLTFAGDTGTPSQRKLGSTVTVKGGVSNESQLTDNNIGVISNGNG
                     SLTVKLAKDIKVNSVTAQTVTANIAVADTVKTGDTTIDTNGLTIVGGPSITKTGINAA
                     GTKVTNVKAGTEDTDAVNFSQLKATEKNINNKINNIDSKVNKVDKRLRAGIAGATATA
                     GLPQAYLPGKSMLATAGDTYRNEAAIAVGYSRISDNGKVIYKLTGNSNTRGDFGGSIG
                     MGYQW"
BASE COUNT         4501 a         3316 c         3496 g         5051 t
ORIGIN      
        1 ggtattttag cgtcagctgc atcagtaatt ttgcctcttt atgcagcata taaagactat
       61 caatttgggg agaatatgtt atctcttatg gatataatta ttttcccgtt aggtattgtt
      121 cgtggactta tgtatttatt tggatagatt atgaaaaaat atttattttt tgcttttatg
      181 acattaagta atgttgctta tcctaattta gatgagtggt actcagcttc agataaagag
      241 cgaagagaat ttatcgcaaa acattttgca gaagaaacag taagaagtga tagaaataca
      301 ccgtatgatt atgctatgtg cactggtaga atgtttgcta aacaactgac tcgagatgaa
      361 gtcaaagact accctttggc aaacactaag ccgcaatatt taacccgtga tagacggatt
      421 gagattatgt ctaatttacc tcgtgaaata ttacaaaatt gtgctaaata catacagtaa
      481 ttttatgagt aacaccactt tggaattaag tggtgttatt ttaattttaa taatgtctct
      541 aaaatcatct ccaacataaa ctcaataaag ccggtagaat cattttggct gtctgcgaga
      601 gaaagcattt gataataacg ttgttgattt tcgtaaacca ttgtttcaat cggcaaccaa
      661 gcgaaaagtg ggttccattg tgctaaaagg acattttgcc ataatcgacc cattctgccg
      721 tttccatcac taaaggggtg aatcatttct atttcatagt gcataaccga gcttttaatg
      781 agtgcaggtg tatcatcttg tttagcccaa gagaacaact cattaatcaa attctctacg
      841 aattgaggac gagcccccat atgcacaagt tgcccactga aattataaat gccgacatct
      901 tttgaacgaa aagcccctgc gtcatttact aagtcagcag taagaaactt atgtgctttg
      961 agaaaatctt taatcgagta aggattatag tgcatcattt catcgtaagc gtcataggca
     1021 tttttgactt cacgaatatc tttggggtgt ccgagaacct ttttgccctt aatgattgcg
     1081 gtaacttgct caatcgttaa tgaatttgcc tcaatagcta aagaggcttg aattgatctc
     1141 aagcggttat tttttctaag atgtaaatta cgctcaaaat ccaattgtag cgttcccaat
     1201 ttatgagaaa tttctacaac cagattcagc attttagtgg taactgtaaa aggtggttta
     1261 taagccattt tgcaataatc ctaaaaaatt aaaccgcttg tcattgttac tccaccttcg
     1321 gcttatcgcc gttttcaaca cagcctttgc cgatggtgac ttttttcgca ttgagggttg
     1381 ggagatcgta cattgtgtca agtaataagt tctcgacaat agaacgtaac ccacgagccc
     1441 cggttttgcg gctaatcgct ttttgggcaa ttgaaattaa tgcatctttg gtgaagtgca
     1501 attcaacgcc ttccatttcg aataaggctt gatattgctt gatgatcgca tttttcggct
     1561 cggttaagat ttgaatcaga gcttcttcgt ccaattcttg cagcggagtc accaccggta
     1621 aacgcccgat taattcagga attaagccga atttgactaa atcttccggt tcaacttgtt
     1681 tgaatagctc ggttaaatct tggcgatctt tatctttttt caatgccgct gcaaaaccga
     1741 taccaccttg agtgttggta cgggcttcca cgattttatc taaaccggca aaagcaccgc
     1801 cgcaaataaa gagaattttg gaggtgtcca ccggaatggt ttcgcctttc gggtgtttgc
     1861 gaccgccttg tgggttaatg ttcgccaccg tgccttcaag tagttttaat aaggcttgtt
     1921 gaacaccttc gcccgataca tcacgggtaa tggatgcact ttccgattta cgggtgattt
     1981 tgtcgatttc atcaataaaa acaatgcctc gttccgcttg ttcggcttca aaatcgcatt
     2041 tcattaataa tttttgaatc acgttttcga catcttctcc cacatagcct gcttgggtaa
     2101 gggtagtagc atcagctacc gcaaaaggta cgtttaaacg gcgggcaagg gtttctgcca
     2161 acaaggtttt accgctaccg gtcggcccga ttaataaaat attactttta cctaactcta
     2221 cgccattagt ggcttgatgg tttgacaatg cacttcttaa gcgtttgtag tggttataaa
     2281 cggccaccga aagcactttt ttcgcgtgtt cttgaccgat gacataatca tctaaatgag
     2341 cgtgtaattc gtgaggtgtc ggcacattgg tgaaaaaact gccttcatcg tcttggtttt
     2401 catcaattaa cggctcttct tcaccgtgta ataaggcgta ggattcctcg atacattcat
     2461 tacaaatata gccttccgtg ccttcgacta attgatcgac ttcggtacgt tttttaccgc
     2521 aaaaactaca gtggggttct ttttcaaatg ccattacgcc acctcactgc gagaagttaa
     2581 tactttgtcg attaagccat actcagcggc ttcttccgca gacataaaat tatcacgctc
     2641 ggtatcggct aacacttttt catagctctg accgctgtgt tctgccatac ggcgggtaag
     2701 ggtagctttc aagcgtaaaa tttcttccgc ttgaatttga atatcggttg cttgacctct
     2761 cgctccacct aaaggttggt gaatcataat acgagcgtgc ggcagagcaa agcgtttacc
     2821 ttttgccccg gctgaaagca aaaatgcccc cattgaagcc gcttgccctg tgcaaagggt
     2881 tgctacatcg ggtttgatga agttcattgt atcgtaaatt gccaagcctg ctgttaccac
     2941 gccacccggt gagttaatgt ataaatagat gtctttttcc ggatcttcgg cttctaaaaa
     3001 cagaagttgt gccacaatca ggtttgccat attgtcttcc acctgaccgt ttaaaaaaat
     3061 aatacgttct tttaataaac gtgaataaat gtcgtaagaa cgttcgcctt ttgaggtctg
     3121 ttccaccacc attgggacta atgccatttt gttttcctct gtcgtaatac aaaatctcgc
     3181 tattctagca aattttcaga aaatcattaa caacaagcgg tcagattgtt aaaatctttt
     3241 gcaaatgatg aggaaattat gacattaaca gaaatttacc aaaaatttga acgttgtaaa
     3301 tcgtgggaag aaagataccg cttgttaatt cagctaagtc gcttgttacc aaaaccaagt
     3361 gagcaagagt tggcacaaat agcggaaatt gagggctgtg aaagccgttt atggtttgaa
     3421 tttcaagctg agccacgaaa agtagtggca tatagcgatg cccgtttaat gcaggggatt
     3481 ttatttattg tgagtgtagc tttaactgaa aaaacggcag aagagctgaa aggctttgac
     3541 ttaaaaacga tgtttgatga gctaaaaatt tcacaaaatt taaccagcac aaggttaaat
     3601 gggttgggtc agttacagaa acatataatg atgaatttgt aattcaccct cgccccttgt
     3661 gggagagggt gaataaaatc cgcaggattt tattcgggag aggggaatat aaaaaacctc
     3721 tgatttctca gaggcttaaa tttgttggcg gaacggacgg gactcgaacc cgcgaccccc
     3781 tgcgtgacag gcaggtattc taaccagctg aactaccgct ccatactggt gctaaaataa
     3841 tagtggtttc ctcaacattg agcaaatagt ttttgcaaaa tagtgtttaa ttggttgtaa
     3901 aataaccact ttatttctgc ttgatcgcct tgataaacag caacggcgga cggtgctgta
     3961 aatctttaaa ttcattgtgc caccgaggtt ggcctgccag cattggttct tccatttgca
     4021 caatctcaaa cccgccttga ataaggttat tcacaatggt tgccattgtg cggtggtagg
     4081 ttttaaaggg ttgttgaaac caacttctgt cccgttcgcc ctcttcccga taatagttca
     4141 agcggtaagc aacttgttgt ttttcttggt ttttctccca acgatagcct tctcggtaac
     4201 aggtgacaat cgggtgttct tgcgagaaaa tcagcgtgcc acaagcagtc attttttgcg
     4261 aaatttttgc aagtagttcg gaaaaatcag caatataatg aaacgcaaaa gagctggtta
     4321 ctacatcgaa atcgccctcg ctgatctcat ttaatcgctc catcggcagg cagtaaaggg
     4381 aaaaatcgtt gcctttttgc caatgagcag aaagatcgct ttctgcctgt tgtagcatta
     4441 attcagataa atccaacccc accacttttt ctgcaccgag cgttaaataa tgggctaaat
     4501 gaactcctgt gccacaacct aaatccagca cttttttgcc ggttaagtct ggcagtagag
     4561 agaacattgt tggcttttcc accacttcat tcatacttat tggattttgt cgaagttgtt
     4621 ggtaacgctc aaagaaaatc ggagtgtcgt aaatgctttt tttcacttaa tagcctttaa
     4681 acaaatataa aataaggcgg gttaaaaccc gccactacga tatgattagt aagtcgataa
     4741 ttttcgcaaa gtttctaacg cttttccact ctcaagcgtt tcttttactt tttgcacatt
     4801 ggcttttaaa tcatcttctc caaaggtttt cattaataat gcgacatttg ctgccaccgc
     4861 atcaatatga gccagtttac cttttccttg taaaatagcg gttacttttt cggcattttc
     4921 tgcaggttca ccgcctttga gatcttctaa cgaatggcgt tcaatgccga aatcttccgg
     4981 cgttaaggtg taatagtcga tctgtccgtt ttgaatttcc gcaacctgcg tttcgccgtg
     5041 aacggcaact tcatctaaac ctgcaccgta taccacaata gtgtgttcgt aacctaaggc
     5101 ttttacggtt tcagcataga ctttcactaa ctcaggggag tacacaccga gtaattggcg
     5161 tttagcgtga gccgggttta ccaacggccc taaaatattg aaaatcgtgc gggtttttaa
     5221 ggcttggcga acaggggcgg catatttgaa tccgctgtgg taggttgggg caaacaaaaa
     5281 gcagatgttg gtttcatcta aagcaaggcg tgccgtttca ggtgaaacgt caacattaat
     5341 acctaaatgg cttaatacat cgcttgagcc ggttttactt gatacgctac ggttgccgtg
     5401 tttggcaatt ttatagccca ttgtggcggc aacaatcgca cttgtggtag aaatgttgat
     5461 ggtattatgc ccatcgccac ctgtgccgac aatatccgca aaagggtaat caggcgttga
     5521 gaacggattg gcattttcaa gtgcggcttt tgcagcacct gcaatttcat caacggtttc
     5581 gccacgcagt tttagagcga ttaaggcagc agcaagttgt tcatttgata cattgccttt
     5641 aataacttca ctaaaaaaac ggtgagattc cgcttgggct aaggcttggt tggcgaataa
     5701 tttatctaaa agtgcttgca tagtctcttc ctttttaatt agcgatgtgc ctgatattcc
     5761 aaatcggttg cctcgttgcc ggtcattcca cgaaagcccc agcaatgctc tggctgttct
     5821 ttaatggtga tttcaacgtc cattggcaca atgccgactt tggtgtgtaa ggcactaaat
     5881 agtcctttaa ttaagcgttt tttgagctgt tcgctccgcc cttgcatcag gtttatttca
     5941 atcacaataa aatcttggct acggttaatt gggggataga aattttccgc atccaataag
     6001 tcaaaacgca aagcgtggcg ttgttttggt acgccaaggc tcatttgcat acagtcaaaa
     6061 atcacgtctg caatcagggc tctgcggtct gctaaggttt gttttaatcc atatacacta
     6121 atcatttttt tctcctaata accattccac cgactgctta agtaattttg tgccttgcac
     6181 ggttaaaatg gattccgggt ggaactggaa accgcaaatc ggtaaggttt tgtggcgaat
     6241 tgccatcaca atatgttcaa atttcgcatt aacgataaat tctgccggta attcatcgcc
     6301 cattaatgag tgataacgag cgacaagcat tggattgtta atgcctgaaa acatcgcttg
     6361 gttatcgtgt tcaatacgag aaactttgcc gtgcaatact gtgccggtat gcactacttt
     6421 gccgccaaag gcctcaatta gagcttgatg tcctaaacaa atgccaatca tcggtacttt
     6481 gtctttcaga cggcgaataa tttccaataa attgcctgcc tcagccggat tacccgggcc
     6541 gggggaaagg gcaaccaaac aattttcggt ggtttcagct ttggctaaaa aatcctcaag
     6601 cggataatca ttgcggaaaa tcgttacctg atgccctaat tcacgaaatt gatcgaccaa
     6661 gttataggta aaagagtcga aattatcaat aaaaagaata tttgccatag tttgtcctta
     6721 tgcttttgca ttcacctgcc caatcgcttt taacaccgcg cgtgctttat ggcgtgtttc
     6781 atctgcctct aattgtggat cggaatccag cacctcacca caaccagctt gcacataggc
     6841 aatgccattt tgaacaaagg cggaacggat cacgatacag gtatctaaat tgccgtctga
     6901 ggttaaatag cccaccgcac cgccgtagct atggcgtttt tgttgctcga cttggtagat
     6961 cagttgcatt gccttaattt tcggagcacc agttagcgtt cccatattca tacaagcttg
     7021 ataagcgtgc agagcgtcaa gatcttttct aagtgtgccg atcacacgag aaactaagtg
     7081 cataatttgc gaatagcggt caatttgcat taaatcaacc acacggcgag tgccggattc
     7141 gctcacacga gccacatcat tgcgggcaag atcgactaac attaaatgtt cggcaagctc
     7201 tttttggtct aaacgcagtt ctaattccaa acgggaatct aattcaggat cgatattacc
     7261 ttgttcatca aaaccgcgtg ggcgagagcc ggcaatcggg taaatttcca actggcggtt
     7321 ggcttgcgaa tatttcaacg cactttcagg cgacgcccca aacagagtga aattttcgcc
     7381 ctgcatatag aacatatacg ggcttggatt gttgattttg agctgtttat agctggcaag
     7441 agtattcggg caaggcaagc tgaaacggcg agagggcaca atttggaaca catcaccgat
     7501 attaatatgg tgtttgagtt tgcggataat cgctttgaaa tcttcatctt caatattggc
     7561 ggttgcctca ctcgttgcgg tttggttcgc tagaggcagc tcaatatgtg acagatttgc
     7621 aagtttttgc gtaaattcga ccgcttgttg gttcaaattg gtttgttcat tcggttcaaa
     7681 acagaaggtg tttagcaccg cagtttcggt ttggtggtcg attgtaacca gttgctctgc
     7741 taaatagaaa gtgtaatctg ggcaacttaa gccatcatct tgcaggctga tattttccat
     7801 cggaataaaa tttgccacca aatcgtagct aaataatccg cccaaataga taggcgatgt
     7861 ggaatcagca aacagttggc tcaccgttct tagcccgtca aacacggtgg tggttttgag
     7921 cttgctgtct tcatctaact ggttgctgat ggttgggaaa atcgcttgca agcggtcgtt
     7981 tttgtctaaa aatttgcaag tcggagcagg gtttaacagg ctcaatgcgt ttgcaatcaa
     8041 aggtaaaacc gcttctccgt ttggggttaa agcgtggaaa gtcacggttt gatcttcaca
     8101 gaaaatatgc actgccgatt tcgcaaacag caaacttttt acactgtttt tactttgaat
     8161 ttccgctgaa tctaataata gcgtatgtgg gcgttcgccg cataatgcgt aataaacacg
     8221 ggtcgtatcg gcaaaatagg gtaattctgt ttgagtaatc gttaattgag gcatagcaaa
     8281 ttcctattca atttttcgta ctattaaact agtacattaa aaagaagtca agcaattatt
     8341 ccagataatt atgcgagctt gggcgaactt ggtgtataat tagcccaatt tttagaaagt
     8401 cgaaggcttg agactatgac acaatttttt tatatccacc ccgacaaccc acagcaacgc
     8461 ttaattaacc aagcggtaga gattattcga aacggtggcg taattgttta ccctaccgat
     8521 tcaggctatg ctctcggttg tgcaatgggc gataagcacg caatggatag aattgtagca
     8581 attcgtaaat tgccggaaaa tcacaatttt accttagttt gtagtgattt atccgagctt
     8641 tctatctacg cgttggtcaa taatcaagcc tatcgcttaa ttaagaataa taccccgaat
     8701 ccttatactt ttattctgcc ggcaactaag gaacttcctc gcaggctgat gacaaagcgt
     8761 aaaacagtgg ggattcgtgt gccggataat gcgatcgcat tggcgttaat ttccgcctta
     8821 ggtgagccga ttctttcttg ctcgttaatg ttgccggaga cggaaatgac cgagtcagac
     8881 cctgatgaaa ttcgggatca tttgcaaaat cgggtggatt taattatcca cggtggttat
     8941 ttagggcagc agcctacaac ggtagttgac ttaaccgatg acgtgccggt tattattcga
     9001 gaaggatcgg gcgatccaac atcgtttaat taaattgatg ttgtatgatt aatatataga
     9061 attatgcgta ggggggctgt gccagtccgt attaatatcg ttatttggac aggcacagga
     9121 cctgtcccta caatgaaaat caaattcaga cgcttgtgaa agcgacaaaa ggaaccttat
     9181 gacaactttt caaaaaaaat caactgcgac tttccgtgca gacaaacgtg gtgaaagccg
     9241 tccctcattt aaaaaagaag agcaaaaatc tgaacgcaaa tttggtaaaa cgtcagaaaa
     9301 accaccacgt ccaacttcta agccattaac gcaaagaatt gcaaaaaaag tggaaaaaac
     9361 gaccgctagt gaagtggcat ctcaagagaa aaaagccaaa gtagtaggtg aaaagctaca
     9421 aaaaatctta gcgcgagcag ggcaaggttc tcgccgtgaa ttagaagaga ttatcgctgc
     9481 tggacgagtg agcgtggacg gtaaaatggc tactcttggt gatcgtgtgc aggttagctc
     9541 atcgactaaa atccgcattg acggacattt agttaattta atgccggcac aaaaagagat
     9601 ctgccgtgta ttaatgtatt acaaaccgga aggggaactt tgcacccgtt ccgatccgga
     9661 aggacgatcc accgtatttg accgcttgcc tcgtttaagt ggcgctcgtt ggattgcggt
     9721 cgggcggtta gatattaaca cctcaggctt attattgttc actaccgatg gcgaattagc
     9781 caaccgttta atgcacccaa gccgtgaagt cgaacgtgaa tattcggtgc gtgtgttcgg
     9841 caacattgac gatgcgatgt tacaacgctt acgcaaaggc gttcagttgg aagacggacc
     9901 ggcaaacttt aaacaaatta aggttgtggg tggcacaggc ttgaaccaat ggtttgatgt
     9961 gactttaacc gaagggcgta accgtgaggt tcgccgcctg tgggaatcgc aaggggtaga
    10021 agtcagccgt ttgattcgta tccgttacgg caatatcaaa ttagataaag gcttacctcg
    10081 tggcggctgg gaagaaatgg gcttagaaca agtcaattac ttgcgtgagc tggtagggtt
    10141 accgccggag actgaaacta aagtggaagt gggggtaaac cgccgccgaa ccaatatccg
    10201 tcaaattcgt aaagcggtaa aacaacatca aaaatatcgt ggctaaacac catctttggg
    10261 gctgcattag ttctgctaat gcagctattt tctttcaaaa aaggagcatt tatgtcttgg
    10321 gatattgttg caacattggc ggtcactggg attgccgtat ttttatttgc aactgaaaaa
    10381 atgcgaatgg acgtagttgc gattttagtg ctgtgttcat tagtattatt agggcaggtt
    10441 gataccaaat ctgctctgag tggattttca aactcagcta ctgtaaccgt aactgcaatg
    10501 tttgtattag ctgctggttt acaaaatagt ggtgctttag atagtgtagg cagtttactt
    10561 gctaaggtaa aatctccttg gttattcttg cttgtactct gtggcgtaaa tgctgctgtt
    10621 tctccttttg tgaataatac ggcagtggtc gcagtattaa ttccgattgt gattgccgca
    10681 gcacagaata taaaaatggc tccttccaaa gcccttattc cgttatcatt ttcttctcaa
    10741 atggcaggtg tttgtacact gatcggcact tccactaatc tattagttaa tgcaatcgct
    10801 caaaagcaag ggcattccgg tttcggtatg tttgaatttg cccctttggg gattattttt
    10861 ttagtggttg gggttgccta tttattagtg accagccgtt tcttattacc cgaatcacat
    10921 ttgcaacttg aagatggtga ggggtttgga aaatatgttt ctgaacttaa agtaaataaa
    10981 gactctgtat taattggcaa aagtacattt gaatcaggat taaatgaaga atttaatcta
    11041 tttactattg gtgttttgcg tgatggggaa agattatcga caccgagtca tcaagtattg
    11101 caaaaacatg atattttatt gttgcggggt gaatcggaaa atttagcaaa agtacgagaa
    11161 aaatatggat tgcaccacgt tgtgtatgga cgccgtgaga atgatgaaga taatctggat
    11221 gaagacttga tggtggctga agtgatgatt tctcctattt ctcgttggat aggagggact
    11281 attcctattt tacaacagcg ttggaataaa aatgcaacgg tgttaggcat tcaacgccaa
    11341 agtaaagtaa ttcgtgagcg tttacgcacc actgctttta aaatggggga tattttatta
    11401 ctcacattac caaaagagga tatggagacg ttacgccaag ataaagactt tattgtgtta
    11461 tcttctgatt tagtcaaaac ggaggaatcg tggaaaagta aatttgcttt aggggtaatg
    11521 gttttggtgg tggcaacagc agccttaggc tgggttccta tttcagtgag tgccttaatt
    11581 ggtgcagttg cgatgtgtgt tgctggttgt ttaacggctg aagaagccta tcaatctatt
    11641 gactggaaaa tcattttagt cttagctgga ttactgccat taggtgaagc gatggcaaat
    11701 agtggggcgg cgcaatttat tgttgataac acgcttggta aagtagggga gttcgggcct
    11761 ttagtggtgt tagctgtatt atatttactg acaatggtgt taaccgaatt tatgagtaat
    11821 gcagggactg ccgtgttgct tacgcctatt gcggtttcta ccgctaaaat gttaggggta
    11881 gatgcttccc cattcattat tgccgtaatg tttgcggctg cgaccagctt tatgacccct
    11941 gttggctatc aaaccaacac gatggtatac ggtgcaggtg gctataaatt cacagatttt
    12001 attaaaattg gtttaccgct aaatgtaatc tattggattt tagggatagt attaattccg
    12061 attttcttcc cttttaatcc ataattgata gataattata gaaaaaaggt ttacagatca
    12121 gaaactctgt aaaccttttt tattcagcaa taaatcaatt ttctattgga aaattaccat
    12181 tgataaccca tgccaatact accaccaaag tcgcctctgg tattgctgtt accagttaat
    12241 ttatagataa ccttcccgtt atctgaaatc cgagaataac ctactgcgat tgcagcttca
    12301 ttacggtatg tatctccagc cgttgctaac atacttttac ctggtagata tgcttgcggt
    12361 aaacctgcgg ttgcagttgc accagcaata ccagcgcgta aacgtttatc aactttgttt
    12421 acttttgaat caatattatt gatcttatta ttgatatttt tttctgttgc ttttaattgg
    12481 ctaaagttaa ctgcatcagt atcttcagtt cctgctttta cgttagtcac ttttgttcct
    12541 gctgcattaa tacctgtttt agttatactc ggtcccccta caatagttaa tccattagta
    12601 tcaatggttg tatcaccagt cttaacagta tcggctacag caatgttagc tgttactgtt
    12661 tgagctgtga cagaattaac tttaatatct ttagctaatt taaccgttaa tgaaccatta
    12721 ccatttgaaa taaccccgat attattatca gttagttgtg attcgttaga taccccacct
    12781 tttactgtaa cagtagaacc taatttacgt tgagatggcg tgccggtatc acctgcaaat
    12841 gttagtgggc tggttactgc atcatttaac gcagtaattg cagagcctac attattgacc
    12901 gttttactaa cttcatttgt aggagcgcca tcaaccacag tatagcttgg ggctgaaaca
    12961 gcaccatttg catttacact tgaattacca cctaaagcgg tagcaatagt atttgccgta
    13021 tcgtaaagtt ggctaccatt aactgcatct gctgagatag gtgaaatatc accatcagct
    13081 acattagtta tttttaattt gccagcatcg acacctgtca ttgtgacact tgggccatta
    13141 ttaatggtta agccgttgtt atttagtttt gaattaccaa cagctacgga gccggcagga
    13201 gttaaatcaa tgtctttcgc taatttaact gttaatgtgt tagcatctgt tgccactaca
    13261 ccgatattat tatcagtcaa tatggttgca tcggccccac ctttaacatt cagagaagta
    13321 cctaatttac gaacaatttc agtaccagca tcatctacaa aagtaagtgc tccattactt
    13381 aactcattca ctttcgtgtt taaaccatca atttcggtat aagcagcata aagttgtgaa
    13441 ccgttgatag catccgttga tgagtccaaa atacgacctg ctgcaacatt cgttaaagta
    13501 cgttcattgc ctacagaacc aatacttaca gttgagcttg gcgtagtacc tgcaaatgtg
    13561 tatgtattgc catttagcgt tgcacttttt gttgcaactg tggctactgt ttcagagtta
    13621 gtacctaatg caacagaacc ttggtgtgtt gcttttgcat tttggccaag agccactgca
    13681 gcagttgttg ttgcttgtga tgaaacacct aatgcaatag aagctacacc ggctgcattt
    13741 gcactattac ctaaagcaat cgcagagcta cctgcagcat tggcatttct acccacagaa
    13801 gttgaccata ttccacctgc attagcagag ttacctaaag cggttgactg tgtacccgaa
    13861 gcatttgcgt tatagcctgc agcaagagaa tctgcaccga ttgaacttgc ttcataacct
    13921 acagcggttg aattttcacc ggtagcttca gatttagagc ctaatgcagt tgcacgggta
    13981 gctgtcgctg ttgaagctgt accaatagct gttgaggtat cagcatttgc attggctgca
    14041 gaaccaaccg cgagggcatt tgttgcattt gcattcgcat tgtagcctat agaagtggca
    14101 ttcataccat cagctgttgc gcgaggacca actgcagttg tacgttcacc gtttgcttct
    14161 gcttcagagc ctaatgcggt tgccagctca ccattagcac ttgttgcaac gccgatagcc
    14221 attggattaa caccgctaga gcctgaatta tcatagttag cacgttgaat gccatcatct
    14281 gaaatactaa cataatgagt tgcaaatttg ctgatagctt gtttgagttg actgacattg
    14341 accgcatcag tatctacatc gcctgcggca acattggcga ttttattacc gccattatct
    14401 aagccagatt tagtaagact taccggtttg tctgcaacgc cgttagcgat ggtgatacct
    14461 gtattgttaa caacggtatc acctgttgtt aatgaaccat tagcgcctaa gttaactttt
    14521 tctgctaatt ttatggttaa agtactattg ccatcagcta caacaccaat attattctcg
    14581 gttaatatgc ctgtagatcc gcctttgatc ttgactgttg aacctagatg gcgctcaaaa
    14641 ttagttcctg tatcaccggc aaaggttaac ggactgatca ccgctgtgtt taatgcgctt
    14701 agtgctgcag aaacagtagt gtatctccct tgtttgcctt ctttatctgc atctggtgtg
    14761 ccattcacta aagcgtaggt tgggaaagtg atatttccat tttcagtaac agctgcccca
    14821 ccacctaaga tatttgcggt tgttgcagca acattaccta tagcttgttg tgttaaatat
    14881 aattgtgaac cgtttatggt atctgtagag cttaaagata tacgtcctgc agcaacattg
    14941 gtgatagttc tctctttgcc ttccgcccca atacttactg tagcaattgg atttgttcca
    15001 gcaaagtcac tatatgtaat accatttaca gttgcatttg tagttggtat ggctatagct
    15061 gtaactgagt tgctacctag cgctatcgaa tcggtatgtt cagcagtggc atttcgacct
    15121 atagcggttg cattaaaggc ggaagcattt gatgattctc cgattgcaat tgtgcgttga
    15181 tttgttgctt tagcgcttac ccctagtgca acagaagccg cgcctgatgc agtagcacga
    15241 ttgcctaaag cggttgcaga acctccactt gcattggctt cacgaccaaa tgcagaagca
    15301 taatcgctgg ttgcacccgc attagtacca acagcagtag ttaaatttgc tgttgcattt
    15361 gcataataac ctaatgaggt tgcaccgtgg cctgaagtct tcgcttcata accaattgct
    15421 gttgagttat cgccatttgc ttgagatttt gcaccaattg ctgtggagtg aatagctgtt
    15481 gcgttagctt cagcaccata ggcggagcta acaataccag ttgcattggc atgactacct
    15541 acagcagttg cttttcctat taccgtttct cctgttgctg ctgtagtttt gtaattatta
    15601 acaccatttg caagagcgga attaccgatg gcgatattat catcactggt tacatttgta
    15661 gtaggtcctg ctagttttgc attagcataa gtaccaattg caatattgtg gctaccacta
    15721 gtaccgtctc cagcagtatt accaatagaa atcgtattgt ttcctgttac attagagcta
    15781 gccgaataac caataccaat attgtattct gtgctgttag agtgatttaa tccggcatga
    15841 tcgccaataa aaacttcacg taagcccatt gcggttgcat tgtaccctac tgctacggaa
    15901 cgtttatcgg cagtggcttg cccaccaaca gctgttgaag caaaactagc gttagcaaag
    15961 cctcctaatg cagttgaaaa atcggctgtc gcggttgtgt tataccctaa tgctgtagaa
    16021 actttaccgc tatttccttc taagcctgtt attgtgctta ctagtgttga gttaacttta
    16081 gatccatcac caattgcgat agatgtttgt gggttattat ctggtatggt cccataggtt
    16141 ggtgaatagg ttgcagcact tgcaaaacct gcagaaagcg caagacttag tgtgctgagt
    16201 ttaataatat tagtggttag tgatgtattt gttgcattag atttattagc acttcttgat
    16261 aattcagaag caacaatcca agttcgtttt gcatggttcc aaacaatgcg ataaatttta
    16321 ttcatagaac tactcctgtt cttatttttt aataatttta atga
//
LOCUS       DANLXQ010000044        16347 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.044, whole genome shotgun sequence.
ACCESSION   DANLXQ010000044 DANLXQ010000000
VERSION     DANLXQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16347
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.044"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..2167)
                     /gene="yccS"
                     /locus_tag="PXD94_001057"
     CDS             complement(<1..2167)
                     /gene="yccS"
                     /locus_tag="PXD94_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL6285935.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLSRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGVRGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVALSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNGLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLGIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSHAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLTQFEQENQQKQNDLALVLIQQLRLIVQLLPQLNSI"
     gene            complement(2211..2669)
                     /locus_tag="PXD94_001058"
     CDS             complement(2211..2669)
                     /locus_tag="PXD94_001058"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL6285936.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFNQAISVNVPDYDGYLAERLG"
     gene            2795..4069
                     /gene="thrC"
                     /locus_tag="PXD94_001059"
     CDS             2795..4069
                     /gene="thrC"
                     /locus_tag="PXD94_001059"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL6285937.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLDSIDEL
                     LDLPLVKRSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVEHILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
     gene            complement(4148..4882)
                     /locus_tag="PXD94_001060"
     CDS             complement(4148..4882)
                     /locus_tag="PXD94_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL6285938.1"
                     /translation="MSFKDMTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLP
                     GAFTPTCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPD
                     GNGEFTEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTM
                     IKYLSPNWTAKESVSIITKPGCLFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITG
                     KTSVPQVFIGGKYIGGSDELAAYFAK"
     gene            4997..5881
                     /gene="oxyR"
                     /locus_tag="PXD94_001061"
     CDS             4997..5881
                     /gene="oxyR"
                     /locus_tag="PXD94_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL6285939.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEGELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            5884..6516
                     /gene="fabR"
                     /locus_tag="PXD94_001062"
     CDS             5884..6516
                     /gene="fabR"
                     /locus_tag="PXD94_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL6285940.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(6543..6842)
                     /locus_tag="PXD94_001063"
     CDS             complement(6543..6842)
                     /locus_tag="PXD94_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL6285941.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWNALSEHHQLALEWLKLSTAYITKE"
     gene            complement(6931..10746)
                     /locus_tag="PXD94_001064"
     CDS             complement(6931..10746)
                     /locus_tag="PXD94_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL6285942.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVADAIN
                     NSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGRDNDGNMTVNTDNVTIV
                     KDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIANAINNSGFNVTAGDGVD
                     GGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDNVAFNSIDMSNGKPDTT
                     GSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNNAVNVGDVQAAMKAATT
                     KVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTSELTNNPDGKVEE
                     PTEPNALVTAKTVADAINNAGFNIKANGDEKSLVKTGDTVQFLNGKNIEITRDGNNIT
                     VSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKVTNVAAGDVNADSKDAVNG
                     SQLYTFAMASREEVKSTDKSVTVNTTKNADGANVFDLSVNTDDVTIVKDPTTGAIKAN
                     TTALNDANNDGRIDEPTADDAKKLVTAGDITNAINNSGFTLKTSAVEGGEKLSGGDEL
                     INPGKAVEMVAGKNLTVKQEADGKVIYATKDDVKFSSVTSNTVTVPTDEADPANNPIT
                     INKDGINAGNKAISNVASNLIPVTADDKVQPADNNPTNLADKLSNAATLGDVLNAGWN
                     LQGNGKAVDTVVHNDTVDFINGKGTTVTVENKDGKNTIKVDSPIEFVNQDPTDSSTPS
                     NTAKFTGEAPVQLGNVASSVRNEDGSTPEGKDRAEAIKNAEGDKLNNVVNLGDLQAAT
                     NAATTKVGGNRGVTITPSTNADGSTTYNVEAKTDGTTIKVDNEGNITANTSELGNNED
                     GTVKAPTQPNALLIAQTVADAVNNAGFNIKSAGNKAAGDQAATKLVKTGEEVVFEAGD
                     NLTVKRDGNQFTFATAKDVSFNSVQFSENGPKITNDGDNIKVGDKDGKPTKITNVADG
                     DISPVSTDVINGKQLNNYAKVNGNNIGTDEDGSINIVNGNGTTITSDKAGEVKVNVNI
                     TDLTVADNGKINVQDPNGTGSRFVNATTVANAVNNVSWNVDSKAVGTGAVEGDKAPAK
                     VKAGSTVSVNAGNNIKVTRKGSDVTVAVSDTPEFTSKTGDTLVNNNGVTINNGSAGKA
                     VSLTKDGLNNGGNRITNVKAGEADTDAVNVGQLKGAVNHLNNKIHRNNREARAGIAGS
                     NAAAALPQVYIPGKSMVAAAGGTFKGENALAVGYSRSSDNGKLILKLQGNANSRGDFG
                     GGVGVGYQW"
     gene            complement(10709..11194)
                     /locus_tag="PXD94_001065"
     CDS             complement(10709..11194)
                     /locus_tag="PXD94_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285943.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADGDQEAAELINPGEKVIFAAGDNLKVKRVGTIFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            complement(11203..13362)
                     /locus_tag="PXD94_001066"
     CDS             complement(11203..13362)
                     /locus_tag="PXD94_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6285944.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGAMGDKSVANATYSLALGSSANANATSTATRGTAIGYNATVSGVN
                     ATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANVALNNSVALGSNSTVSNAVAV
                     PSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQISATSTDAINSSQLYM
                     AMNATGNLANSTKNILGGNATVKPDGSVTYTNIGGTNKNTIEEALKAVKTEVVAGSNV
                     NITNATGANGQTIYTVNAYNTTANSSSPDYITVTGKAATAANTTNYEIGLTKKAIDDF
                     TKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNSTVPENQAN
                     DGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVATVSKGIQATAVGHS
                     ANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASLAVGANSI
                     ASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTENATVGGIT
                     YNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINGSQLYMAMNATSNLA
                     NSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNYVNQGFNI
                     KDNAGETKGTVTPNESVQFVNGKGTVSNVTQEADGVK"
     gene            complement(13591..13791)
                     /locus_tag="PXD94_001067"
     CDS             complement(13591..13791)
                     /locus_tag="PXD94_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL6285945.1"
                     /translation="MNQIFKVIWNQATQSWVAVSELQRAKGKTKSVSSASKATLALLA
                     GASAVLGANDAVAVVSYRFNNK"
     gene            14155..15309
                     /locus_tag="PXD94_001068"
     CDS             14155..15309
                     /locus_tag="PXD94_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL6285946.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTALYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALEAFLKTLTDERYRK"
     gene            complement(15357..16346)
                     /locus_tag="PXD94_001069"
     CDS             complement(15357..16346)
                     /locus_tag="PXD94_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL6285947.1"
                     /translation="MNDLIIYNTEDGKSNVALLVVENEAWLTQNQLAELFDTSVQNIS
                     EHIKNILKDKELDENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRK
                     WANSTLRTYLEKGFLIDSERLKNPQGRLDYFDELLEQIREIRASELRFYQKVRELFKL
                     SSDYDKTDKNTQMFFAETQNKLIYAITKHTAAELICQRADATKMNMGLTSWKGKVVRK
                     GDIVIAKNYLDNNELDSLNRLVMIFLESAELRVKNNQDLTLGFWRRNVDMLIEFNGFS
                     VLGNGGTITHKQMESFVREQYEKFDIQRKCLKQKEADWEDLQALEKLESELTR"
BASE COUNT         4641 a         3704 c         3013 g         4989 t
ORIGIN      
        1 atatactatt taactgcggt aataactgca cgatcagtct gagttgttgt atcaacacta
       61 aggcgaggtc attttgcttt tgctgatttt cttgctcaaa ttgggtgagg tcttggtcga
      121 tagcggtcaa tttttccgat aattttgcaa atgaacggct ctctgaggca atttgcccga
      181 tgatgtttgc cacctctctg gcgtgtgaga agaacattgc ggaaaaatcc acttgttgat
      241 taatctctcg gctagcaata cggtaagccc ctaaggtgga aatataaccc agcaaggtgt
      301 aagcaatacc taacaatttt ggggcgaagg ttaaatcgtt cgcatatttt tgtggttcgc
      361 tgtacatatt ggtaatcgca ttactcagtg ccgataaatg gttatgcaca tcacggcggg
      421 caatacggta aggcagttgc tcgttataac caaactgcaa ctgggcgata atatggcgta
      481 aatattgccc actggctttc aagccgttat gtaagttacg ttgtaaattt aaatacttcc
      541 aatccggcca taagaatgcc actgccagcc acgcaatcgc tgtacctagt aaggtatcaa
      601 gcattcgcat taagatgccg ctttcaaggc tcacgcctgc aatatcaagg cttatcatta
      661 ccaacacagt gatataaaag gttgaaaagc cataatgtac caagcggaaa aaggtataaa
      721 gcgaaccggt cagtacgatt agcccaagct gggcttcgag gctgagcgcc acgttaaaca
      781 gttcaaaaga gccaccaacc agcacgccga aaattgtgcc gatcacacgc tgaatcagtc
      841 gggtcttagt agcagtgtaa ttcggttggc agacaaaaat ggctgtgagt aaaatccagt
      901 agcctttgct atcaaagcct aaaatcggca caatcgcact gcacaacagc accactatcg
      961 acaaccgaac ggcgtgtcga aacagttggg attcaaaggt taaatgggct ttaattgctt
     1021 ggctgatatt gcttaagccg gtaatatttt cggcgatcaa gcggtgagat tttgtgaaat
     1081 ttttacttaa cggttcaata tgctcaccac tgcttgagcc ttgttcaatc tgggctagtt
     1141 gccgctcaat attgcctaaa ttttctgcaa tagcacgcca gcgataagca cttttcaagc
     1201 cccgttcttg atgataattc aatgaattaa gcaagccctg caaggctttt tcgccacgaa
     1261 cgccgtgttc atatacttct ctatggcgta acgacattgc aattttttga cacgccgcag
     1321 cctgtaattc cagcacccgt tgaaagcgga acattaaatc actgttactt aattgttgga
     1381 acagttcgtg atattgataa tggctggaac ttgcccgctc taaaatatct tgagcggtga
     1441 aatagtaacg caacattcgc tgtgtccgtt gctgcttgtg ctggcggctt aggcggtaga
     1501 atagggcaac tctggtttta tcaaaggcag ccatcacctc actattggct ttcgccaagg
     1561 ctagttgttt ggcagctaaa tcgtcatcat cagggtcaaa ataggcagat ttcgccagta
     1621 aataatggct caacgcaagg taggcggacg ctaaattctc ctgcacggtt cgattcggga
     1681 aaatcaggta caccaaaata ccgaccaaac catagagtaa ggtgccaagt aagatcatca
     1741 ccacattgtg ataccacaat acttcgggcg tataagccag gctgttataa accgccacaa
     1801 tcagcgtgcc aaaagcaatg gtactatagc gttgcccgat tgcccctaac attaccagca
     1861 aaaaggtgca aaccaccgcc accggcacaa acagccagcc caaatggagc gacacactcg
     1921 ccccaatcga ggaaagcgag aacgccacta ggctgataat cagatttttc gctcgtccga
     1981 ttaggctgtt gtctaaatcc accaaaccgc cggcgataat gcctaacatc aacggcatcg
     2041 caaaatcaga aataccgagc tgccaaatgc tgaccgccgc aatgtttagc gaaataaaca
     2101 tcggcagggt tttaattaca ttttccgaaa gccaactgtg ttgtagatat cttaaaaatt
     2161 tattcatatt tgctcatttc gggcaggcac aggacctgcc cctacctaca ctaccccaaa
     2221 cgttctgcca aatagccatc ataatccggc acattcacgc tgattgcttg attgaacaaa
     2281 tccgcattta acagaaaatt agcggtggtt tcgttaatcg ccaccggaat attccaaacg
     2341 gtcgcaatcc gcatcagggc tttcacatca ggatcgtgcg gagcggcatt catcggatcc
     2401 caaaagaaca ccagcagatc aattttcttt tctgcgatca gcccgcctaa ttgctgatcg
     2461 ccccccatcg ggccgcttaa caatgaagta atttcaagcc ctgtttctct ggcgagcaaa
     2521 tgcccggtag tgccggtggc atatagctta tgcgaggcaa gttgggcttg atgagtttgt
     2581 gtccaacgaa ttaaatcttg tttacagcta tcgtgagcca ctaaggcaat ctgtttttgt
     2641 tttgcgatgg ttctgacggt ggttttcatt tttagctcct ttgcaaaatt tttcacaaat
     2701 cataccgctt atttttcgct tttgcattga ctaatcacgg cttatggcta aaatcaggta
     2761 aaaattcgca ttttaaaaca aaggattaac cgcaatgaac ttatacaaca tcaaacaccc
     2821 tgaagaacaa gtcaatttcg cccaagcggt acgccaaggc ttaggcaaaa atcaagggct
     2881 atttttccct gaagtgttac ctaaattaga tagcattgat gaattattgg atttaccttt
     2941 ggttaaacgc agccaaaaaa ttctttcagc cttaatcggc gaagagttac cgcaagaaac
     3001 cttaaatgca atggtaaaaa atgcgttcac tttcccggca ccgcttgcca aagtcaatga
     3061 tgatatttat gcgttagaat tatttcacgg tccgacttta gccttcaaag attttggtgg
     3121 gcgctttatg gctcaagcct tagccaatgt acgaggagac ggcaaaatta ctattttaac
     3181 cgctacctca ggtgacaccg gtgcggcggt ggctcatgct ttctatggtt tggaaaatat
     3241 caacgtggtc attctttacc cgaaaggcaa aatcagccca ttacaagaaa aattattctg
     3301 taccttaggc ggtaatattc gcactgttgc gattgaatcc gactttgatg cttgccaagc
     3361 cttagtgaaa caagcctttg atgatgccga gctacgccaa gcgattggtt taaattccgc
     3421 aaattccatc aacattagcc gtttactggc tcaaatttgc tactactttg aagcagttgc
     3481 tcagttacca aaagaaaaac gagataacgt agtggtttca gtaccaagcg gtaatttcgg
     3541 taacttaacc gccggcttaa ttgccaaaac attaggttta ccgattaaac gttttattgc
     3601 ctcaaccaat gccaatgaca ccgtgccacg ctatttacaa tcaggcaaat gggaaccaaa
     3661 cgcaaccgtt gcaacccttt ctaatgcaat ggacgtaagc cgcccgaaca actggccacg
     3721 agttgaagaa ttatttaaac gcaatggctg gagtttgtct gaattgggtt ctgccgcatt
     3781 aaacgatgcc gaaaccgaag cggctttgaa agctcaatat gcagaaggtt atctgtgtga
     3841 gcctcacggt gctattgctt accaaagctt gaaagatcag ctacaacaag gcgaaaccgg
     3901 tattttctta tgcaccgctc acccggcaaa attcaaagag tcggtggaac acattttagc
     3961 gattgaattg ccgttaccgg aagcattaga taagcacaac aaactgccac tgttatccga
     4021 tgaaatggca gcagattttg caacattgcg tgcatatttg ttgaaataaa tattcaataa
     4081 tagaaaaggg attaagtatt aaacttaatc cctcttttta tgcattacaa gcggttatat
     4141 ttgcctatta ttttgcaaaa taagcagcta actcatcact accgccaata tatttaccgc
     4201 cgataaatac ttgtggcaca gaggttttac ctgtaattgc tcgtacagaa attgtgcttg
     4261 catcacgacc taacacaatc tcctcaaaag tatagccttg ctcttttaat aatgcttttg
     4321 cttttgcaca gaacaggcaa cccggtttag tgataatcga aacagactct ttagctgtcc
     4381 aatttggact taaatattta atcattgtat cggcatctga tactttaaac ggatcgcccg
     4441 gttcttgtgg ctcaataaac attttttcca caataccgtt tttcaccagc atcgaataac
     4501 gccaagaacg tttaccaaac ccaaggtcat ttttatcaac taatagcccc ataccttctg
     4561 tgaactcacc attaccatca ggaataacag taatattttc agattcttga tctgctttcc
     4621 aagcgttcat tacaaaggta tcattaactg acatacagat gatatcatct acccctaatg
     4681 ccttaaattc acaagctaat tcattatagc gaggcaaatg tgttgatgaa caagtcggtg
     4741 taaatgctcc gggtaatgaa aacacaacca ccgttttgtt attaaataac tcatcggttg
     4801 tgacatcaac ccaagcatcg ttttgacgtg tacgaaatgt tacactgggt actttctgac
     4861 ccgtcatatc tttaaaagac atattaatgt tctcctatag aattatatat tacaaaaatc
     4921 ttaggcatta tactcacaaa tatggcataa ttcagcctat cagaacaata ggaaaaattt
     4981 aaattggagg aatcaagtga atattcgaga tttagaatac cttattgcac ttgcagaaca
     5041 taagcatttc cgtcgtgcag cagaatcttg taatgttagc caacctactt taagcgggca
     5101 aattcgcaaa ttagaaggcg aactaggcac tattctgctt gaaagaacca gtcgcaaagt
     5161 cctctttacg caagcaggct taacattagt tgaacaagcc aaggtagttt tacgtgaagt
     5221 aaaaattcta aaagaaatgg caagcaacca aggtaaagag atgtctggcc cgcttcatgt
     5281 tggtgtaatc ccaaccttgg ggccttacat ttcgccaatt attgtgcctg cattaaaagc
     5341 agaatttgca gatttagagc tgtatattta cgaattacaa accaaccaat tattagatca
     5401 acttgaatcc ggccagcttg attgtggcat tatctcattt gtaaaagaga gtgaatcttt
     5461 tattgaagtg cctatcttta atgaagaaat gtacttggta gttccagaac aacacgaatg
     5521 ggcagcgctt aaaaaattcg atatgaattt attaaaaaac cgtgagttgc tctttttaga
     5581 cagtggccat tgtttacgca ctaatacgct agactattgt ctatccattg gagcaaaaga
     5641 aaatccacac tttaaagcta ccaaccttga aaccttacgc aatatggtag cagcaaatgt
     5701 tggcctagca ttgattccta aattagccgc taaaccgatt aaaggagtac attatattcc
     5761 acttgaaaac ccgaaaccgt ttcgagctgt gggaatggtg tatcgaccag gttcaccatt
     5821 acgcgcccgc tatgagcgtt tagctcaagc cattactaaa attatgcaat ccgaggcgta
     5881 aaaatggcaa acggtatgag tgtgcgcgca attcagaaag aaaaaacacg cagagcatta
     5941 gtagaagccg cctttaatca attaagctca gaaaaaaatt tctctaatct cagtttgcgt
     6001 gaagttgcac gtgaagctgg agtcgcaccg acttcgtttt accgccactt taaagatatg
     6061 gacgaactag gcttagcaat ggtggacgaa tccgggctac tattacgcca attaatgagg
     6121 caagctcgaa aacgaattgc aaatggcgga agtgttgtcg tggtttcagt ggaaacattc
     6181 ttcgaactga ttgccgaccg ccctaacgtt ttccagctat tactacgcga aagctccggt
     6241 acatcgcaag cgtttagaac cgcagcatca cgcgaaattc aacattttgt agctgaattg
     6301 gcggactata ttatgagcaa agataatcac agtaaccgag aacttgctta tattcaatcg
     6361 gaaggtttag tcacgctagt atttactgcc ggttctcatg ctttggatat gaacaatcaa
     6421 gaacgggaaa aactaaagca acgtgtcatt atgcagctaa gaatgctgat tcgtggctcc
     6481 acctattatg ccaataaaca agcttcatca acataaaaaa acagacgcct aagcgtctgt
     6541 ttttattctt tcgtaatata tgcagtcgat agctttaacc attctagtgc taattgatga
     6601 tgttcagata acgcattcca taacgcatct tctgacaaga tactataatt gcctttagta
     6661 tctaactgtt catcaaaact atcatgtaat tcacgccatt ccgggctagc ataaaaccga
     6721 cttaagggtt ccaaaagtgc agaagctcgt ttccattgct caatcccctt ttccaattct
     6781 ggtaatagct ctacccattg gttataacaa tcctgcattt tctgaatatc tgcttttttc
     6841 attctttacc gcttacacca taaattttag cctcaatata acataaaaat ccccttccga
     6901 agaaggggat ttcaactcaa ttaggctaaa ttaccactgg tatccaacgc ctacaccgcc
     6961 accaaagtcg cctcggctat tcgcattacc ttgtaatttc aagataagct taccgttatc
     7021 acttgaacgt gaataaccta ctgcaagcgc gttttcacct ttaaatgtac cacctgctgc
     7081 agccaccata gatttacctg gaatgtaaac ctgcggtaac gctgctgctg catttgaacc
     7141 ggcaatacca gcacgggctt cacggttatt acgatggatc ttgttattta agtgattaac
     7201 agcacctttt aactgaccaa cattaacagc atcggtatct gcctcacctg ctttaacatt
     7261 agtaatacgg ttaccaccat tatttaagcc atctttcgtt aagcttaccg cttttcctgc
     7321 agagccatta ttaatggtta cgccattatt gttcaccaat gtatcaccag tcttgctagt
     7381 aaattcaggg gtatctgaaa cggctacagt tacatcagaa cctttacgtg tcactttgat
     7441 attattacct gcattcacgc tcactgtact acctgctttt actttcgcag gggctttatc
     7501 cccttcaacc gcacctgtac caactgcttt agaatctacg ttccagctta cgttgtttac
     7561 ggcatttgct actgttgttg cattcacaaa gcgtgaaccg gtaccgttag gatcttgaac
     7621 attgattttg ccgttatcag caacggttaa atctgtgatg tttacgttca ctttcacctc
     7681 accagcctta tcactggtaa tagttgtacc attaccattt acgatattga tagaaccatc
     7741 ttcatcagta ccaatgttat taccattcac tttcgcatag ttgttcagct gtttaccgtt
     7801 gataacatct gtgcttactg ggctaatatc gccatctgct acattagtga tcttagttgg
     7861 tttaccgtct ttatcaccga ctttgatgtt atcaccatca ttagtgattt tcggaccatt
     7921 ctcactgaac tgtacgctat tgaagctcac atcttttgct gtcgcaaaag taaattgatt
     7981 accatcacgt ttaacagtta agttatcacc tgcttcaaac acaacttctt cgcccgtttt
     8041 cactagctta gtcgcagctt ggtcaccggc agccttatta cctgcagatt taatattaaa
     8101 gcctgcatta ttcactgcat ctgcaacagt ttgagcaatc aataacgcat ttggctgagt
     8161 tggtgctttc accgtaccat cttcgttgtt acctaactca ctggtatttg cagtgatgtt
     8221 accttcatta tctactttaa tagtagtacc gtcagttttc gcttcaacgt tataagtagt
     8281 tgaaccatca gcattcgtgc taggtgtgat agttacacct cggttaccgc ccactttcgt
     8341 agtagctgca ttagttgccg cttgtaaatc acctaagttc actacgttgt ttagcttgtc
     8401 gccttcagca tttttaatcg cttctgcacg gtctttaccc tctggggttg agccatcttc
     8461 gttacgaaca ctacttgcca cattgcctaa ttgaacaggg gcttcacctg tgaatttagc
     8521 cgtattgctt ggcgttgagc tatcagtagg atcttggttt acaaactcaa tcgggctatc
     8581 tactttaatc gtattcttac cgtctttgtt ttcaaccgtg accgttgtac ctttaccgtt
     8641 aataaaatca accgtgtcat tatgtaccac agtatcaacc gctttgccgt taccttgtaa
     8701 attccagcct gcatttaata catcacctaa tgttgctgca ttacttaact tatcagctaa
     8761 gttggttgga ttattgtctg ctggttgaac cttatcatct gcagtcacag gtatcagatt
     8821 agacgcaaca ttgctaatcg ctttattacc tgcattgata ccatctttat tgatagtaat
     8881 tggattattt gcaggatctg cttcatcagt tggtacagtt acagtgttag aggttacgct
     8941 gctaaactta acatcatctt tagtcgcata aatgacttta ccatcagctt cttgcttcac
     9001 agttaagttt ttacctgcaa ccatttcaac tgctttaccc ggattaatca gttcatcacc
     9061 acctgaaagc ttctctccac cttctacagc cgacgttttc aacgtaaagc ctgagttatt
     9121 gatggcgttg gtaatatcac ctgctgtcac taatttctta gcatcatcag cggttggttc
     9181 atcaattcta ccgtcattat ttgcatcatt taatgccgta gtgttagcct tgatcgcacc
     9241 ggttgtagga tcttttacaa ttgttacatc gtccgtattc acagaaagat cgaacacatt
     9301 cgcgccatca gcattttttg tcgtgttaac cgtaactgat ttatcagtcg acttcacttc
     9361 ttcacgagat gccatagcga aagtataaag ctgactacca tttactgcat ctttactatc
     9421 tgcatttacg tcaccggcag ctacattagt tactttgact gcattaccgt ctttgtcacc
     9481 aacattgata ttaccaccat tgttagtaat cttaggacct tcatttccga actgtacact
     9541 atcaaaattc acattttttg cagttgaaac agtaatgtta ttcccatcac gggtaatctc
     9601 aatgttttta ccatttaaga actgaaccgt atcacctgtt ttcaccaggc tcttctcgtc
     9661 accgtttgct ttaatgttaa aacctgcatt gttaattgca tctgcaacgg ttttagctgt
     9721 tactaatgca tttggttctg taggctcttc cactttacca tcagggttat ttgttaactc
     9781 gctggtgttt gccgtgattt cgcccttatc gtttacctta atggttgtac catccgtttt
     9841 tgcttcaacg gtataagtgg ttgagccgtc atcgttagtt ttagatgtta cagtaacacc
     9901 tttatcacct tccactttgg tggttgctgc tttcattgct gcttgtacat cacccacatt
     9961 cacagcatta ttcaacgtat cacctgttgc gttgttaata ttatccgcaa tcgctttacg
    10021 ctctgcatct gttggtgctg ctttatctgc aaatgtacca cccacgccgc tcttcagatt
    10081 agtgattgag cctgttgtat ctggtttacc attgctcata tcaatgctgt tgaatgcaac
    10141 attatctttc gttgtgtaag tgaatttacc gtcagcttgg gtaagtgtca tatttttacc
    10201 cgcatcaaag gtaactgttt cacttggctt gattagctgt gttttcttac ctttggtttc
    10261 accaccatca acaccatcac ctgctgttac attaaagcct gaattattga ttgcattagc
    10321 aatatcacct gccgtcacta atttcttagc atcgtcacct gtaggctcag taactttacc
    10381 ttcaggagaa ttatttaatg ttgtggtatt cgctttaact tcgcctgttg tcggatcttt
    10441 cacgatagta acgttgtcag tattcactgt catattaccg tcattatcac gaccgataga
    10501 tttatccgtt gcaacatcta ccgtatattc agtattgcct ttatcatctt cctttccggc
    10561 tttaacagtt acggctttag aaccagctac tacagatgag gttttctctg aattattaat
    10621 tgcatcagcc acattaccac gagttactgt aggagcacct gctttcgcta catctaccgt
    10681 atagattgtt tgaccattat cacctgtttc aggtgcacca acagttgcta aacctgaacc
    10741 tttcacttcg gttttgtgac gctgctgctt gttttaactg acttacattc accgcatcag
    10801 tatcagcttc acctgctttc acgcctgtaa ttttggttgg attaccatca ttacctgcaa
    10861 tattaacatt accatcttta ttcgtgattt tcgggccatt atcaccaaat gttacgctat
    10921 caaatttcac atctttcgct gtttcgtacg tgaatatagt gccaacacgt ttaactttta
    10981 agttatcgcc tgctgcaaaa atcacttttt cgcccggatt aatcaactct gctgcttctt
    11041 gatcaccatc agctttattg ccaccagatt ttgcattcca acctgctttt tgaatcgctt
    11101 cagaaactgt ttttgtagtt gcaactttat cttcttctgc aggtgtagtt actgcacctg
    11161 ttgtgttatc aaccgtgata gaaccggtat ccacatcaaa ggttacttta cgccatcagc
    11221 ttcttgtgta acatttgaaa ctgtaccttt accgtttaca aattgtacag attcatttgg
    11281 ggtcactgtg cctttggttt cacccgcatt atctttaata ttgaaacctt ggtttacata
    11341 gttatttaag ttcgtaattg catcgccaac gtttgttgca gtattaccat actgagttcc
    11401 agctgcattc gttacattat atttaggctg agtcacagag ccatccggat tcacaacaga
    11461 accgccacca aaattatttg cagttgaatt tgcaaggtta ctggttgcat tcattgccat
    11521 ataaagttgt gaaccattaa tcgcatcggt tgatgtagca gaaatctgac ccgctgccac
    11581 attttggatt tgacgctctt tacctgcaga gcctacagaa acaacatagt tagtatcttt
    11641 atttaccccg gcaaagccat tataagtaat accccctacc gttgcatttt cagttggtgt
    11701 tgctgccctt acgactgatt cgctacccaa agcaacagaa ctattcagag tagcttgagc
    11761 tcccatacct aatgctgtag caaattgagc tgttgcatta gcacgtgtac caacagccac
    11821 tgctgattgt gctgaggcaa ttgaatttgc acctacggct aaagaggcat ttgcactcgc
    11881 atttgctgca acaccaaagg cagaagcttt ttcagccgtt gcattagcat tagaacctac
    11941 cgctgtagca tcacctgctg tcgcatttgt ttggcgacca agtgcggttg taccattagc
    12001 cgttgcattt gctgaatgac caactgccgt agcttgaata ccttttgaaa ccgtagcaac
    12061 acctaaagat aacgctccgt taccatttgc atttgagttt gtgcctaatg ctactgccgc
    12121 ttgctctcca cctgttgccg ttgcattcgg gccgatcgca attgagtttc tgctcttcgc
    12181 gccatcatta gcttgattct caggtacggt tgaattcaca gagaaatatt gaatttggct
    12241 tgcttgcgca ggaatattga cagaaagatc ataaataacc gtgccatttg catttctctc
    12301 tgtacttttt acagttactg taccatcgtt tgaaacaaca gtagcttggg tatctttcgt
    12361 aaagtcatca atggctttct tagttaaacc aatttcatag ttagttgtat tcgcagcagt
    12421 tgcagcttta cctgttacag taatgtaatc agggctagaa gaatttgccg tggtgttata
    12481 cgcatttact gtataaatag tttgcccatt tgcaccggtt gcatttgtaa tattaacatt
    12541 gctacctgca acaacttctg tttttaccgc ttttaatgct tcttcaatcg tatttttatt
    12601 tgttccacca atattagtat aggtaacaga accatcaggc tttactgtag cattaccacc
    12661 taaaatattt ttagttgaat tagcaaggtt acctgttgcg ttcattgcca tataaagctg
    12721 tgaactatta attgcatcgg ttgatgtagc agaaatctga cccgctgcca cattttggat
    12781 ttggcgtttg atcgtgtttg agccaacaga aacaacattc cctgctgccg tagtattagc
    12841 cgcaaagccg gaataagtaa taccatttac cgtcgcagaa ggaactgcca cagcgttgga
    12901 aacggttgaa ttactcccta aagcaaccga attatttagc gctacattag cacctgagcc
    12961 taaggcaata gcaaaaccgg cacttgcttt agcactatca ccaattgcaa ccgtagattg
    13021 gttggaagat actgcactta cccctaaagc tgtcgcatta acacctgaaa ctgtcgcatt
    13081 ataaccaatc gctgtacctc tagttgcagt agaagttgca ttagcattag cacttgaacc
    13141 taaagctaaa gaataagtag catttgcaac agacttatcc cccattgcac ctgaacccat
    13201 tgcaaatgaa cgagtgccac tagcggcaga tctccatccc attactgttg aacggttacc
    13261 agtagctaga gctcctgcac caaaagctgt tgcacggtta cctgttgcag cagtatccgt
    13321 acctaatgca atagcatttg ctgaagtcgc tctagccccc ataccaatag caatagaagg
    13381 aactaatgcc caattctgac cagtgttatt gagactctct acaccaatat tatagtaatg
    13441 taagaagtat cttctggatc cactaaattt gttctatgcg ttgcattcgt atctgtacca
    13501 attgcaatag tagatctgcc agttgagatt gaatccgcac caatagcaat tgcgttctcc
    13561 gatgatgcag ttgcttgtgt tgaaatataa ttacttgttg ttgaacctat aagatacaac
    13621 agccacagca tcattagctc ccaacacggc actagctcca gccaataaag ccaatgttgc
    13681 cttacttgca gaactaacag atttcgtttt acctttcgct cgttgtaatt cagatactgc
    13741 aacccaactt tgtgttgcct gattccaaat aactttaaaa atttgattca taatcgaccc
    13801 tatataaaaa taaaaaagat tacgtagaac ctatgttcaa cataatcttc accaatataa
    13861 aaaaccgcca cctatttaag tgaaataact tacttataga ctcagaaatt taagtgcgcg
    13921 tattttacgc tatttgtaat ataattcatc ttctggagtg taaatttatt gtaaataata
    13981 cgaggaatct aaccgcttgt tataacgcaa aacaccttga cttaaatcaa gccactacct
    14041 cattttgagg ggagatttat acgcagtgaa attttaaaat aagctaaggt ttacggataa
    14101 tcgttcaaca ctattgaatt atcactgtag ttatcgttaa aaaggtatgc ttttatgaac
    14161 caattcttcc ctctaatctt tagcttaact ctattttccg gcgtaacttt ggcactagaa
    14221 aaagcacctc gaactatgcc gacagaagga ataaataagc ccctacttgg acaaattcta
    14281 ttctttgaca atatgctgtc acacaacggt tcacaaaact gctccagctg ccataatccg
    14341 gattatgcct ttattgataa ccgcactact ccggcaaaag gggtggtttc acaaggagat
    14401 actccaacca aattcggcaa acgcaactcc ccgaccatgc tttatgcgaa atactcgcct
    14461 gagttccact ttgatgagca aagtcaagaa tatatcggcg ggcagttctg ggacggacga
    14521 gccaaaaatc tcgccgagca agccggtatg ccgcctatcg acccaaatga aatgggaatg
    14581 cctaacaaat tagaagtagc caaaagatta tataatgtgc cgatgtatca gcgattttta
    14641 gggcaatttt acggtgaaaa ggtatgggaa agttatgaaa gcgtttatgc ggcaatggaa
    14701 gatgcgattg cgacattcca acaagaaaac aaacttctcg ctccatttga ttcaaaatat
    14761 gatctatttt taaaaggcga ggtacaatta actgcattgg aagaaaaagg cagagcttta
    14821 tttttcaata aagctcaaac taattgtagt agttgccacc aattacagag tgctgcaaac
    14881 caccaagaag aaacctttag caactatcac tattacaatg taggcgtacc cggtaaccca
    14941 gacttgatag cggttaacgg cttcgcacca gacttcgtcg atttaggctt atttgaaaac
    15001 ccaaatgtta aaggtgatga aacacaaaaa ggcaaattca aaaccccaac attgcgtaat
    15061 gttgccgtga ctgcacttta tatgcacaac ggtgtgttta aagaattaaa gactgttttg
    15121 ctgtttttag acagctacaa taacccggag cgaaaaatca accctgaaac cggcacggcg
    15181 tggcaacaag cagaatttga acccactatc gcccaccaaa aactcaaagc caaaccctta
    15241 agcgatgaag aaattgatgc cctcgaagca tttttaaaaa ccttgacgga tgagcggtat
    15301 agaaaataag gctacaagcg gtaaaatttt ggggaaattt taccgcttgc gtttaactac
    15361 cgtgttagct cactttctaa cttttccaat gcctgcaaat cttcccaatc tgcctctttc
    15421 tgttttaaac atttacgctg aatatcaaat ttttcgtatt gctctctgac aaagctttcc
    15481 atttgtttat gcgtgattgt tccaccattt cctaatacag aaaagccgtt aaactcaatc
    15541 aacatatcta catttcttcg ccagaaacct aacgtaagat cttggttgtt cttcacacgt
    15601 aattcggcac tttcaagaaa gatcattacc aagcgattta aagaatccag ctcattatta
    15661 tctaaatagt ttttcgcaat cacaatatcg cctttacgca cgacttttcc tttccagcta
    15721 gttaatccca tattcatctt agtcgcatca gcacgttgac aaatcaactc agcggcagtg
    15781 tgtttggtta ttgcataaat gagcttattt tgcgtttctg caaaaaacat ttgcgtattt
    15841 ttatctgttt tgtcgtaatc gctagagagt ttgaaaagct ctcgtacttt ttgatagaaa
    15901 cgcaattcac ttgcccgaat ctcacgaatc tgttcaagta attcatcgaa ataatctaaa
    15961 cgaccctgcg gatttttcaa acgctcgcta tcaattaaaa aacctttttc gagataagtg
    16021 cgtaatgtgc tgtttgccca ttttctaaac tgtactgctc ttgggctacg aactcgaaac
    16081 cctatcgcca gaatcatttc aagtgaataa tgttttactt gataattctt gccatcttga
    16141 gcagttatca agtaatcctt gataactgaa ttctcatcta actctttgtc ttttaagata
    16201 tttttaatgt gttcggatat attttgcaca gaggtgtcaa aaagttctgc aagttgattt
    16261 tgtgtcagcc aagcttcatt ttcaaccact aacaaggcta cattgctttt gccgtcttcc
    16321 gtattataaa taattaaatc gttcata
//
LOCUS       DANLXQ010000045        16276 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.045, whole genome shotgun sequence.
ACCESSION   DANLXQ010000045 DANLXQ010000000
VERSION     DANLXQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16276
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.045"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(401..1441)
                     /locus_tag="PXD94_001070"
     CDS             complement(401..1441)
                     /locus_tag="PXD94_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL6285948.1"
                     /translation="MKKQLRKNLTQNDRLQPQAQTEIFSFGDPIPVLDRADILNYLEC
                     SAMYEKWYNPPMSFDGLAKSLRSSTHHESAIITKANILLSTCEVDSRYLSRRDLSSFV
                     KDYLVFGNAYFEVVRNRLGQVQRIESPLAKYVRKGLEAGQFYYVPQRFDHQEHEFAKG
                     SIYHLLEPDINQDIYGLPQYLSALQSAWLNESATLFRRKYFLNGAHAGFVFYMSDASQ
                     KQEDVENIRQQLKQSKGVGNFKNLFVHAPNGKKDGIQIIPIADVSAKDEFFNIKNVSR
                     DDVLAAHRVPPQLMGIIPNNTGGFGNVADAAEVFFITEIEPLQERLKEFNQWLGQEVI
                     KFKPSKLLQRTQ"
     gene            complement(1450..3252)
                     /locus_tag="PXD94_001071"
     CDS             complement(1450..3252)
                     /locus_tag="PXD94_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDL6285949.1"
                     /translation="MENENTITEYEVLAANPLHDKREAQSKYWAGYTVTEISRQLNIP
                     VSTIASWKKREKWDEISPVGRVEATLESRLNLLIMKESKNNNDYKEMDALRRLLESTA
                     RIKKYSNGGGNEADLNPNIKNRNKGDRKKPEQNAISEEQAELLINGFLDGMFHYQKKW
                     HEAGLTHRIRNILKSRQIGATYYFAHEALVDALVTGRNQIFISASKKQALQFRAYIVA
                     YAKRVADVELKGETITLPNESQLIFLGTNSKTAQSYHGNLYFDEIFWVNRFEEIRKVA
                     AGMASQKQYRITYFSTPSSITHSAYLLWSGKLFNRKRPKAEQVEIDISHANLKNGKKC
                     GDGQWRQIVNIYDAEAGGCNLFDIEQLKLENSPDEFEQLFMCEFIDDNQSVFKFTMMQ
                     RCLVDSMEVWRDYVFTDGYQRPFGNKEVWVGYDPSYTGDRSALVVIAPPKVDGGKFRL
                     LEYRTFKGADFAEQAAEIVAICAKYNVTRLAIDTTGLGVGVYEIVKKERPDAVALTYN
                     VELKSKMVLKGLDIISKGRFEFDSMHAVEVGASFMAIKKQITNSGRQVTYVADRSEEA
                     SHADLAWACLQVFINEPFDGKLEETTGTVDFLDD"
     gene            3402..4229
                     /locus_tag="PXD94_001072"
     CDS             3402..4229
                     /locus_tag="PXD94_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDL6285950.1"
                     /translation="MTTKSKWFVVATEGATTDGREISREWLTQIEENYDPKNNYGARI
                     NLEHFRWRWFEKDDPHSYSYGDVLAVKTEEREDGKLQLLAEIAPTEALVKLVKDKQKV
                     YTSVEIDLNFADTGEAYLVGLAVTDTPASLGTEYLTFCAGATHNPLADRKQKPENLVS
                     EAVEANLEFTAEPEKGAWLEKFKAMFTKAKTDNDGKFAEHEKAMELLAEQFSKVSTEN
                     DRLQTELSTLQIEFTELQKQAGEFAEKFAKLEQEPSANYTPRPKATGAKAGDVQTDF"
     gene            4243..5271
                     /locus_tag="PXD94_001073"
     CDS             4243..5271
                     /locus_tag="PXD94_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDL6285951.1"
                     /translation="MKDLTLEKYNAYLARQAELNNLPFNALATGIKFTVQPSVQQKLY
                     EKVRESSDFLKSISFVFVDEQTGETLGLDSAHTVASTTDTSGDGERKTTSIAKLVKQT
                     YHCQQINFDTHINYKQLDMWAKFPDFQQKVANVAAKQRKRDLIMIGFNGTSRAATSDR
                     NSNPLLQDVAKGWLQKMREDAKERVMNGESTDNQVLVGKGQEYANLDALVMDATEELI
                     DEWHRDDTDLVVITGRKLLADKYFPIVNQQNAPTEELAADIVISQKRIGGLKAVRVPF
                     FPANAILITKLENLAIYVQEGTTRKHIENVPKKDRIETYESENIDYVVEDYGCAALIE
                     NITLKDKE"
     gene            5281..5970
                     /locus_tag="PXD94_001074"
     CDS             5281..5970
                     /locus_tag="PXD94_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDL6285952.1"
                     /translation="MRMSPARAHFLRKTAESESATPNRENLEGLQGYDLVLAQLKAHQ
                     RQLKKIQSIERKIEFKRNNFEQYRPWVEGSLAKGSGVQDTVITTMLIWSIDIGDYPTA
                     LAIAQYVLMHDLAMPEQFERTPATALVEELAEAAKKARDQKQPFEAAVLKQANDLTAE
                     FDMPDPVRAKLLRELGELIQEAEPQTALEHYQRAIALDPQCGAKGLRDKLEKQLAKQA
                     EEPKPAEEQNE"
     gene            6082..6597
                     /locus_tag="PXD94_001075"
     CDS             6082..6597
                     /locus_tag="PXD94_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDL6285953.1"
                     /translation="MEQRNHVISIPKVNGYESSKNAIKPTAESDETITNNGFFPNIEL
                     LEVRNAMRIDGTVINERLKQAVIEAMATVNADLKAYRLNAEQAQKANLQACDDEQING
                     ESVLVYKYKRAVYCLAVANLYERYRSYDSTKDGHDKAEELESTAGDLKRDYHFAVRDI
                     LGENRMISELI"
     gene            6594..6806
                     /locus_tag="PXD94_001076"
     CDS             6594..6806
                     /locus_tag="PXD94_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL6285954.1"
                     /translation="MNTLIARQDDTLDELIFRHYGQTAGLVEQALEYNPELAHLPRLP
                     IGTVVTMPDIESRFATVAKSSVQLWD"
     gene            6812..7018
                     /locus_tag="PXD94_001077"
     CDS             6812..7018
                     /locus_tag="PXD94_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDL6285955.1"
                     /translation="MHKTEQASYFGAIFGFFGSLTLSDWGVVVSILVAILTLLLNWAY
                     RAKEYKLKEREFELKLQNQGKKNE"
     gene            7011..7577
                     /locus_tag="PXD94_001078"
     CDS             7011..7577
                     /locus_tag="PXD94_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL6285956.1"
                     /translation="MSKSVKVGVFVCSVAAIIGTVKQYYSSEIRTSETGLAIIGNAEG
                     CRRDPYKCPADVITVGIGSTEASGEKINVNHKYTDKEIAERWIKDLKIAERCINRYFN
                     GDKMNENQFSAMVSAAFNMGCYNLRFYPNENGKYIQTTIHKYAMAKDFKAMCNRIPDF
                     NRSGGKVLRGLKIRREKEKALCLGQEVK"
     gene            7574..8029
                     /locus_tag="PXD94_001079"
     CDS             7574..8029
                     /locus_tag="PXD94_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285957.1"
                     /translation="MIVKLCQMTNKHFSPLMIVLVLVGVLANFVQFRQNESLKADLQA
                     RTTQNQLIQADNRNLANQLENSQIQLEAYQKQVDDLNQKVLAKMKQAEQRSNEILNEL
                     EKHKKWADSAVPPSVGKLLNQRKRAVSHPQIEPDNLSEKAGVPNTGNRD"
     gene            8178..8399
                     /locus_tag="PXD94_001080"
     CDS             8178..8399
                     /locus_tag="PXD94_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDL6285958.1"
                     /translation="MSDQIDRANELAEKAREAALAKILQNQTACTSLFECEDCGEPIP
                     EKRREMVIGCTRCIECQTIYEHKQKGYRR"
     gene            8396..8881
                     /locus_tag="PXD94_001081"
     CDS             8396..8881
                     /locus_tag="PXD94_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285959.1"
                     /translation="MIKPDRLRAVLTQTIDIFQANPEKLILQYDKGKIKSKGSQSHSF
                     EYHYDLELIVVDFPYHPDVLFVPVLNFVRNEQWELLQNPELQDKIEFEIDHNNHESYD
                     IYIRLPLTERVIVKEQDGHLTATHADEPNLADVSPFTRLTEYEVYLKDELIYQWAEPN
                     E"
     gene            8874..9332
                     /locus_tag="PXD94_001082"
     CDS             8874..9332
                     /locus_tag="PXD94_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL6285960.1"
                     /translation="MSEPIEQVKTAFDSLLNNISKPRRRLMYQQIGRELARSQRRRIK
                     AQQNPDGSAYEPRKKPKKGVKSKIKSGKMFDKITQPRFMRLRLESEGVSLGYEGGDAV
                     IARIHQQGLIGRVRKDWDLKVKYASRELLGFTDDDLQMIEDYMINILAGS"
     gene            complement(9383..9661)
                     /locus_tag="PXD94_001083"
     CDS             complement(9383..9661)
                     /locus_tag="PXD94_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285961.1"
                     /translation="MEILFILLGLIGFGALLAIGFTTFAAIFGVVTTVFLSLLSLLAN
                     MVGLSIIVILISLYVLLPLNTFILIALIFSAILGFLCWYHSDRQSVNQ"
     gene            9700..12612
                     /locus_tag="PXD94_001084"
     CDS             9700..12612
                     /locus_tag="PXD94_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285962.1"
                     /translation="MSNNLKIQVVLSALDKLTAPFKSVVKQTEKLSQSLKVQRDELKA
                     LEKTQSKMQSFKKMDDSIKKSNETILKQTKHLDNLKNKISQMKNERIELKVKMDAKKR
                     EFQQITKGGSAWSARAAFLDKDFEKMQREYEKLGASISSVSRKRTEENRALKDSRHQQ
                     AKQLLYFRSLRRELRASGINIKAFAQSETNLTDKIAKANQLIDQQKAKLEKLNAVKAR
                     NEKYWATVEKVKNTSERLHNLGQRSMISGAAISAPVVGLGKGVVGMAQTAGKFEQFNA
                     ILEVTEGSAEKAKQSFDWVKQFAVDTPSNLDEAMEAFVKLRAYGLDPTNGLLQTLGDT
                     GAAMGKPVMQAVEAIADALTGENERLKEFGIKGSVVKGTNIIEYAYTDKLGKQQVAKV
                     NKNNRKEIEQTLTRIFNEKYAGAMEKQAKTLVGIWAKIEDYWTNFQMQVMESGAFDWI
                     KNKLQWVLDSLDKMAENGELQKWAEDVGAVIQETVQGLWAFGEKIIEVVKWLASLARE
                     NKGLIATFVKWSAILGSSLTVLGAFAVVASFALYPVARLALGIGKFTGASTLLNKALF
                     DSNDKFRLFNKSLYSSGTTAAFVKSKFNQLATLPNVLGSSFNKLGGGIKMLIPQLKTK
                     VFWLNMLKGILGKLWSAIRFIFSPLKLLLGIFSPIGLAITAISVAGVALYRNWEKVRA
                     FFGGFLQGLSKGLAPVLERFKPLGKLFETISNWVKSTFKWLSDLLTPADKTSESLDKA
                     SSAGEKFGLALAGAIDLVTSPVQWLVDQFKWISENMPSWDTIGEGVKKAATAIVPTEH
                     AKQITKTAEMSNNMYDPNYTPPQLEQKWIGGLVGNGKGRGFATGGYTGNGGKYEPAGI
                     VHKGEYVMTKEATARLGVANLNRLNYGKVAGLTALASSVAFAQPMPAVKIDSRPPLTA
                     SQPSPTVAPVSQNIHITINATGGQDPQAIARLVAMELEKQQRQAQARARSSLRDRG"
     gene            12674..12853
                     /locus_tag="PXD94_001085"
     CDS             12674..12853
                     /locus_tag="PXD94_001085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285963.1"
                     /translation="MMFKEKGYDEFVNECVRKGREEHKAGLGFTLEQSRLHIKKTIEQ
                     KSQELNEFQREVVYG"
     gene            12846..13133
                     /locus_tag="PXD94_001086"
     CDS             12846..13133
                     /locus_tag="PXD94_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6285964.1"
                     /translation="MAKITIKPQALSQFDEIVKSVIEFTGFETSGIRLQSDIFDKIEQ
                     IAFMPLSGRAREDGTRETFVRRYRIVYDYDRQADEVIILAIIHSSRLYPRP"
     gene            13262..13867
                     /locus_tag="PXD94_001087"
     CDS             13262..13867
                     /locus_tag="PXD94_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL6285965.1"
                     /translation="MSEPNHAEQIRRLDNIIRLGTIAEVDLTTATARVNSGGITTDFL
                     PWLTFRAGTSQTWSAPTVGEQCVVLSVSGEFTTGVILFGIYTQNAPSQSADEHLFRFS
                     DGAEISYNTASGQLTVKNCKVVIVQATESITLDTPTVKATKNVVIGGNLSVKGTTSSQ
                     GAISTQGAVTAKGDVSGAGISLGSHHHIEQGDGRPTSGAKP"
     gene            13867..14202
                     /locus_tag="PXD94_001088"
     CDS             13867..14202
                     /locus_tag="PXD94_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL6285966.1"
                     /translation="MNRETGEQLEDEVEHIKQSIKDILLTAKGSRVMRRTYGSNLYKL
                     TDKPIAASLIMQLSAACVMALQQWEPRIAITRFKVEFAEDNYNKLICTLDFTLKHRKI
                     SIKGMRFTL"
     gene            14199..15116
                     /locus_tag="PXD94_001089"
     CDS             14199..15116
                     /locus_tag="PXD94_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDL6285967.1"
                     /translation="MSEIVDLSKLPAPKVLEELDYETLLAERKAKFLSLYPESERAVM
                     AARLALESEPITKLLQENCYLQLLERQRINSAAQATMLAYATGTDLDVIAANFNVERL
                     VVQAEDLTASPPIVEELEPDDDFRQRVQLKIESVSVAGPRKAYTFHALSANGKIADVS
                     VISPQPAYVTVTLLSRDGNGAVASEIINAAKIALNDEEVRPIADRVTVQGASIVEYEI
                     NAVLHLYRGPEKEPILKAAKANLEAYINQTKRIGRDVTHSGIHAALHVAGVQNVEILQ
                     PRTDLVLTPYQAGYCVNYTLNVEVSDEYS"
     gene            15103..15735
                     /locus_tag="PXD94_001090"
     CDS             15103..15735
                     /locus_tag="PXD94_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDL6285968.1"
                     /translation="MNTVKALLPPGSSLLEKRAAECLQQAVQNPIKFANLINPHKCPV
                     KFLPYLAWALSVDYWEEHWSEELKRETIIRSFEEHRKKGTASAVRRIVESLGYAFEIR
                     EWFAEKRQRQPGTFRVFVELKDKGISEKIYQELERLIEDAKPVSRHITELAVSATSKG
                     KANIFSGAHSGEIITIYPPKKLPVIVIKSKMDLFSATNSGEIITIYPNKR"
     gene            15738..>16276
                     /locus_tag="PXD94_001091"
     CDS             15738..>16276
                     /locus_tag="PXD94_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6285969.1"
                     /translation="MEQKYKTILTHHGERVIVEALANKIPVPLKEMAIGDGNGSSITP
                     SASQTTLVREVYRAEITDLLEDPQNRHQMIAELLIPENVGGFIVREIGLFDEQGGLVA
                     VANCPENYKPVLEQGSGKVQYYRMILQVSSSDAVTLSINNNIVYATRTEFNEFVNNLS
                     SPDGYKHIGRCKSVAELRT"
BASE COUNT         5179 a         3375 c         3629 g         4093 t
ORIGIN      
        1 agttaaaatc tataatgtgc agtatatgta tatgcaatta aagaataacg atttttcctg
       61 acaagcaata tttttgcgtt gaggtgcgta aaattgcgta aaaagatcga gtttttaaga
      121 tcgctttgcg attatatggg gcttgtaggg atcgcctttt tgcatttgcg tataaatcga
      181 caaattaagt gtgcgggcga ggtgagggat agaccgcgcg gggttggagg tggtgaagtc
      241 gttgaaaaat aaaggaaaaa ttatttttta agtgtatttt atttgttgtt ttgatataat
      301 aaattgccga aatatataat ctaatcaggc aaaacttttg ctttatatat taaaaaagcc
      361 ccaatctagg agctgtattg tcagtttgta aagatctgtt ttattgtgtc cgctgtaaca
      421 atttagacgg tttaaatttg ataacctctt gacctaacca ctgattaaac tctttcaatc
      481 gctcttgcaa tggctcaatc tcggtgataa aaaacacctc ggcagcatca gccacgttgc
      541 caaaaccgcc ggtgttattt gggataatcc ccatcagttg tggtggcaca cggtgagcag
      601 ctaacacatc atcacggctc acgttcttga tattaaaaaa ctcatctttt gccgacacat
      661 cagcaatcgg aataatctgt attccatctt tcttgccgtt tggtgcgtgg acgaacaagt
      721 ttttaaaatt ccccacccct tttgactgct tgagctgctg gcgaatattt tcaacatctt
      781 cttgtttttg tgacgcatcg ctcatataaa acacaaagcc ggcgtgagct ccatttaaaa
      841 aatacttacg gcgaaacaga gtagcactct catttaacca cgcagattgc agggccgaaa
      901 gatattgtgg caagccgtaa atatcttgat taatatcagg ctcaagcaag tgatagattg
      961 agcctttggc aaattcgtgt tcttggtgat cgaatcgttg tggcacatag taaaattgac
     1021 cagcctctaa accctttcgc acatattttg ccaaaggtga ttcgattcgc tgtacctgcc
     1081 caaggcgatt tcgcaccacc tcaaaatagg cgttgccaaa tactaaataa tctttaacaa
     1141 acgatgacaa atcacggcgt gataaataac ggctatcaac ctcgcaagtg gaaagcaaaa
     1201 tatttgcttt ggtgatgatc gcactttcgt ggtgtgttga tgaccttaac gatttcgcca
     1261 agccatcaaa gctcatcggt gggttatacc atttctcata catcgccgaa cactccaaat
     1321 aattgagaat atcggcacgg tctaacaccg gaatcgggtc accaaaactg aaaatttcgg
     1381 tttgtgcttg cggttgtaag cggtcatttt gggttaaatt tttgcgcaat tgttttttca
     1441 ttttttgtcc taatcatcta aaaaatctac tgtgccggtc gtttcttcca gtttcccatc
     1501 aaacggctca ttaataaata cctgcaagca agcccacgca aggtcggcgt ggcttgcctc
     1561 ttccgaacgg tctgccacat aagtcacttg ccgtccgctg ttggtgatct gttttttaat
     1621 tgccataaaa ctcgcaccaa cctcaacagc gtgcattgag tcaaattcaa atcgtccttt
     1681 gctgataata tccaagcctt ttagtaccat cttggatttc agctctacgt tgtaagtaag
     1741 agccaccgca tctggtcgtt cttttttgac gatttcataa acaccgacac caagccctgt
     1801 ggtatcaatc gccagtctgg tcacgttata ttttgcacaa atagcgacaa tttctgccgc
     1861 ttgttcggca aaatcagccc ctttaaaagt gcgatactct aatagtcgga atttcccgcc
     1921 gtccactttc ggcggagcga ttaccaccaa cgccgagcgg tcgccggtgt aagacgggtc
     1981 atacccaacc catacctctt tattgccgaa tgggcgttgg tagccgtccg tgaatacata
     2041 atctcgccac acctccatcg agtccacaag gcaacgttgc atcatcgtga acttgaacac
     2101 agactggtta tcatcaataa actcacacat aaagagctgc tcgaactcat cgggcgagtt
     2161 ttccagtttg agttgctcaa tatcaaacag gttacagcca cctgcctcgg catcgtagat
     2221 attcacgatt tgtcgccatt ggccgtctcc gcattttttg ccgtttttca agttggcgtg
     2281 agaaatatca atttccacct gttccgcttt cggacgtttg cggttaaaca gcttgcccga
     2341 ccaaagcaga taggcggaat gggtaatgct cgaaggggta gaaaaataag taatccgata
     2401 ttgtttttgg ctcgccatac ccgctgccac tttgcggatt tcctcaaagc ggtttaccca
     2461 aaagatttca tcaaaataga gattgccgtg gtaagactgt gccgttttcg agtttgtacc
     2521 caagaaaatt agttgagact cgttcggcaa ggtaatggtt tcgcctttta gctccacatc
     2581 ggcaacccgt ttggcgtatg ccacaatata ggctctaaat tgcaatgcct gttttttcga
     2641 ggcggaaata aaaatctgat ttctaccggt tactaacgca tcaactaacg cctcgtgagc
     2701 aaaataataa gtcgctccaa tttgacgact tttaagaata ttgcggattc gatgagtcag
     2761 cccagcttcg tgccattttt tctgataatg gaacattccg tctaaaaagc cgtttatcaa
     2821 cagttcggct tgctcttcgg aaatggcatt ttgctcgggc tttttgcggt cgcctttatt
     2881 acggttttta atatttgggt tgaggtcggc ttcattacct ccgccgttag aatatttttt
     2941 gattcgtgct gtactttcca acaaccgacg taaagcatcc atttctttgt agtcgttatt
     3001 gtttttgctc tctttcataa tcagcaaatt cagccgtgat tccagtgtgg cttccacacg
     3061 tccaacgggg ctgatttcgt cccatttctc tcgctttttc caacttgcaa tggtagagac
     3121 gggaatattt agctgacgag aaatttcggt cacggtgtaa cctgcccaat actttgattg
     3181 agcctctcgt ttatcgtgta gagggttggc agccaacacc tcgtattcag tgattgtatt
     3241 ttcattttcc ataggtgcga ttatcaaaaa tccaccttgt ttaagcattt gcttgaggct
     3301 gtgaaacccg atttaacacg taccacacat tgaaaccgcc ggcacaaaac aagatcataa
     3361 atccgatttt tacccctccc ctttagagca aggaaaaaca gatgacaaca aaatccaaat
     3421 ggtttgtggt agcaacggaa ggagcaacta cagatggacg cgaaattagt cgtgaatggc
     3481 tcacgcagat tgaagaaaat tacgacccga aaaataacta tggtgctcgt attaaccttg
     3541 agcatttccg ctggcgttgg tttgagaaag atgacccgca ttcttacagc tatggcgatg
     3601 tgttagcggt taaaacagaa gaacgtgaag acggcaaatt acagttactg gcagaaatcg
     3661 ccccaaccga agccttagtg aagttagtca aagacaagca gaaagtttat acctctgtgg
     3721 aaattgacct taattttgcg gacacaggcg aggcgtattt agttggtttg gcggtgactg
     3781 atacaccggc aagcctcggc acggaatacc tcacattttg tgccggtgca acccataacc
     3841 cactagcaga ccgcaagcaa aagccggaaa accttgtgag tgaggcagta gaggcgaatt
     3901 tagaatttac tgctgaacct gaaaaaggtg cgtggctgga gaaatttaag gcgatgttta
     3961 ccaaagccaa aaccgacaat gacggcaaat ttgccgaaca cgaaaaggca atggaactgt
     4021 tagctgagca atttagcaaa gtttcgacag aaaacgaccg cttgcaaact gaactaagca
     4081 cattgcaaat cgaatttacc gaattgcaaa aacaagcggg cgaatttgcc gaaaaatttg
     4141 caaaactgga acaagaaccg tctgccaatt acaccccacg cccaaaagcc actggtgcga
     4201 aagcaggtga tgtacaaacc gatttttaat tgaggatcac gaatgaaaga tttaaccctt
     4261 gaaaaatata acgcctacct tgcacgccaa gctgaattaa acaacttgcc gtttaatgca
     4321 ttggcaaccg gcattaaatt cacagtgcaa ccatcggtac agcaaaagct gtatgaaaag
     4381 gtgcgtgaaa gctctgattt cttaaaatct atcagctttg tgtttgttga tgagcaaacc
     4441 ggtgaaacct taggtttaga ttcagctcac accgtggcca gcaccaccga taccagcggt
     4501 gacggtgaac gcaaaaccac ctctattgcg aagttggtaa aacaaaccta ccactgccaa
     4561 caaatcaatt ttgacaccca tatcaattat aaacagttgg atatgtgggc gaaattccct
     4621 gattttcagc aaaaagtggc gaatgttgcg gcaaaacaac gcaaacgtga cttaattatg
     4681 atcggcttta acggcacaag tcgtgcggca accagtgatc gcaacagcaa cccgttattg
     4741 caagatgtgg caaaaggctg gttgcagaaa atgcgtgaag atgccaaaga gcgtgtaatg
     4801 aatggcgaaa gcacagacaa ccaagtttta gtcggcaaag gtcaagagta tgccaatctt
     4861 gacgcactcg taatggacgc caccgaagaa cttattgatg aatggcatcg tgatgacacc
     4921 gatttagtgg tgattaccgg tcgcaagttg cttgcggata aatatttccc gattgtgaat
     4981 cagcaaaacg caccaaccga agaacttgcc gctgatattg tgatctcaca aaaacgcatt
     5041 gggggcttaa aagcggtgcg tgtgccgttc ttcccggcga atgcgatttt aatcactaag
     5101 ctggaaaatt tagccatcta tgtgcaagag gggacaaccc gcaagcatat tgagaacgtg
     5161 ccgaaaaaag accgtattga aacctacgaa tcagaaaata tcgactacgt tgtcgaagat
     5221 tatggctgtg cggcattaat cgaaaacatt acactcaaag ataaagagta aggtggaact
     5281 atgcgaatga gtccggctcg ggctcatttt ttgcggaaaa ccgcagaaag tgagtctgca
     5341 accccaaacc gagaaaacct tgaaggctta caaggctatg acttagtttt agcccaactt
     5401 aaagcccatc aacgccaatt aaagaaaatc cagtcgattg agcgaaagat tgagtttaaa
     5461 cgcaacaatt ttgagcaata ccgaccttgg gtggaaggct cccttgccaa aggctccggt
     5521 gtgcaagaca cggttatcac cacaatgctg atttggtcga ttgatattgg cgattaccca
     5581 accgcccttg cgattgctca atacgtttta atgcacgatt tggcaatgcc ggagcagttt
     5641 gaacgcacac cggcaaccgc cttggttgaa gagcttgcag aggcggcaaa gaaagcccga
     5701 gaccaaaaac agccgtttga ggcagccgtg ctgaaacagg caaacgacct caccgctgaa
     5761 tttgatatgc ccgacccagt gcgagccaaa ttactgcgtg agctaggcga gctaatccaa
     5821 gaggcagagc cacaaaccgc ccttgaacac tatcaaagag cgatagccct cgaccctcaa
     5881 tgcggtgcaa aaggcttgcg agacaagtta gaaaagcaac ttgccaaaca agccgaagag
     5941 ccgaaaccag cagaagaaca gaatgagtag gcgaaagccg aaatagagcg tatccacgcc
     6001 agccaagggg cggattttaa agggtgattt attcattttg caccctttta aaatccccac
     6061 cccttacctt acggaaacct tatggaacaa cgcaatcacg ttattagtat cccgaaagtg
     6121 aatggctatg aatccagcaa aaatgcgatc aagcccaccg cagaatctga tgaaactatc
     6181 accaataatg gcttttttcc gaacattgag ctactggagg tgcgaaatgc aatgcggatt
     6241 gatggcacgg tcattaatga gcggctaaaa caagcggtga ttgaggcaat ggcaacagtc
     6301 aatgccgact taaaagccta tcgcctcaat gcagagcaag cacaaaaagc gaacttgcaa
     6361 gcctgtgatg atgagcaaat caacggcgaa agcgtgctgg tttacaaata taaacgagcg
     6421 gtctattgcc ttgcagtagc gaacctttac gaacgctacc gcagttatga cagcaccaaa
     6481 gacggccacg acaaagccga agagctagaa agtaccgccg gtgatttgaa acgagattac
     6541 cactttgccg tgcgtgatat tttgggcgaa aaccgaatga ttagtgaatt gatatgaaca
     6601 ccttgattgc acgccaagac gacaccttag acgagctgat ctttcggcac tacgggcaaa
     6661 cggcaggctt agtcgagcaa gccttagaat ataaccccga actggctcat ttgccacgtt
     6721 tgccgatcgg cactgtagtt acaatgccgg atattgagag ccgttttgca acggtggcga
     6781 aatccagcgt gcaactttgg gattaaaaaa tatgcacaaa actgaacaag cctcctattt
     6841 tggggcaatt tttggctttt ttggtagcct caccctatct gattgggggg tggtagtgag
     6901 tattctggta gctattttaa ccttattact taattgggca taccgagcca aagaatacaa
     6961 actgaaagag cgagaatttg agctaaagct acaaaaccaa gggaaaaaga atgagtaaaa
     7021 gtgtaaaagt tggcgtattt gtctgctccg ttgcagctat tatcgggacg gtaaagcaat
     7081 attacagctc cgaaatccgc accagcgaaa caggcttagc cattatcggc aatgcggagg
     7141 gttgcagacg tgatccgtat aaatgccctg ccgatgtaat cacggttggc attggctcaa
     7201 ccgaggcaag cggtgagaaa attaatgtca atcacaaata caccgataaa gagattgccg
     7261 agcgttggat aaaagatctt aaaatagctg aaaggtgtat caatcgttat tttaatggcg
     7321 acaaaatgaa cgaaaatcag ttttcggcga tggtatcagc agcatttaat atgggatgtt
     7381 acaaccttag attttatcca aatgaaaatg gtaaatacat tcagacaaca atccataaat
     7441 atgctatggc taaggatttt aaagcgatgt gtaataggat acctgatttt aaccgctcgg
     7501 gaggtaaagt gctaagaggt ttgaaaatcc gccgagaaaa agaaaaagcc ctgtgtctcg
     7561 ggcaggaggt taaatgattg tcaaactctg ccaaatgacc aataagcatt tcagcccgct
     7621 aatgattgtg ctggtgctag ttggcgtatt agccaatttc gtgcagtttc gccaaaacga
     7681 aagcctgaaa gcagacttac aggcacgcac cacgcaaaac caactgattc aagccgataa
     7741 ccgcaattta gccaatcagc ttgaaaacag ccaaatacag cttgaggcgt atcaaaaaca
     7801 ggtggacgac ctcaatcaga aagtgttagc caaaatgaaa caagcggagc aacgaagtaa
     7861 tgaaatcctt aacgaactgg aaaagcataa aaagtgggct gattctgccg tgcctcctag
     7921 cgttggcaag ctgctcaacc aacgaaagcg tgcggtatca caccctcaaa tcgaacccga
     7981 taatctgtcc gaaaaggctg gagtgccaaa caccggcaac cgagattaac accaacggtg
     8041 atcttgtcaa agcactagac aaaagactca acaccattga ggtatgccaa gtcatcatcg
     8101 aatcgttcga gcattgcatc gagcaataca acaaaatcaa ccaaggcgag tgatcgcctt
     8161 ttttaataag gacgactatg tcagaccaaa tcgaccgagc caacgaatta gccgaaaaag
     8221 ccagagaggc ggcacttgca aaaattctgc aaaatcagac cgcttgcacc agcctttttg
     8281 agtgtgagga ttgcggagag ccaatccccg aaaaacgccg agaaatggtt attggttgca
     8341 cccgttgcat tgagtgccaa accatttacg agcataagca aaaaggctac cgcagatgat
     8401 taaacctgat cgcttgcgag ccgtgctcac acaaaccatt gatatattcc aagctaaccc
     8461 cgaaaagctg attttgcaat atgacaaagg caaaattaaa agcaaaggga gccaaagcca
     8521 ttcgtttgaa taccactacg atttagagct aatcgtggtg gattttccgt atcaccccga
     8581 cgtgttgttt gtgccagtgc taaattttgt gcgaaatgaa caatgggagc tactgcaaaa
     8641 cccggaacta caagacaaaa tcgaatttga aatcgaccac aacaatcacg agagctacga
     8701 tatttatatc cgcctcccgc ttaccgagcg ggtgatcgtg aaagagcaag acggacacct
     8761 aactgcaacc cacgccgatg aaccgaacct tgccgatgtt tcgccattta cccgtttaac
     8821 cgaatatgag gtttacctca aagacgagct gatttatcag tgggcagagc caaatgagtg
     8881 aaccgatcga acaagtaaaa accgcctttg atagcttact gaataatatc agcaaacctc
     8941 gcagacggtt gatgtatcaa caaatcgggc gagagcttgc ccgaagccaa cgcagacgga
     9001 ttaaagcaca acaaaaccca gacggctcgg catacgagcc acgcaaaaag ccgaagaaag
     9061 gggtaaaaag caaaattaaa tcgggcaaga tgtttgacaa aattacccag ccacgtttta
     9121 tgcggctacg ccttgaaagc gaaggtgtca gcttaggtta tgagggtggt gatgcggtca
     9181 tcgcccgaat ccaccaacaa ggcttaatcg gacgagtgcg gaaagattgg gatttaaaag
     9241 tgaaatatgc cagccgtgag ttgctaggtt ttactgatga cgatctgcag atgattgagg
     9301 attatatgat taatattttg gctggtagct aaatgatgaa tagaaacttt tgcaaaaaag
     9361 cggtcaattt tgaccgcttg agttattgat taacgctttg tctatctgaa tgataccaac
     9421 aaagaaaccc taaaatagct gaaaaaatca atgctatcaa aataaaagta ttgagtggta
     9481 acaatacata aagtgagatc agaataacga taatagagag cccaaccata tttgccaata
     9541 atgaaagcag gcttaaaaat actgttgtaa ctacaccaaa aatggcagca aaggttgtaa
     9601 aacctattgc caataatgca ccgaaaccaa ttaacccaag taagataaat aagatttcca
     9661 tagattttcc cttagccata ctacaattaa ggttataata tgagcaataa tttaaaaatt
     9721 caagtagttc tttctgcgtt agataaatta accgcaccat ttaagagtgt ggtaaaacag
     9781 acggagaaat tatctcaatc tttaaaagta cagagagatg aattaaaagc attggaaaaa
     9841 acacaaagta aaatgcaatc atttaaaaaa atggacgata gcattaagaa atccaatgaa
     9901 actatactca aacagacaaa gcatttagat aatctcaaaa ataaaatcag ccaaatgaaa
     9961 aatgaacgaa ttgagctgaa agtgaaaatg gatgcgaaaa aaagggaatt tcaacaaatt
    10021 acgaaaggtg gttcagcttg gagtgcaaga gccgcatttt tagataaaga ttttgaaaaa
    10081 atgcaaagag aatatgaaaa actcggtgca tctatttcaa gtgtcagccg aaaaagaaca
    10141 gaagaaaatc gggcattaaa ggattctcgc catcaacaag ctaagcaact tttatatttt
    10201 cgtagtttaa gacgtgaatt gagagcaagc ggcattaata ttaaagcttt tgctcaaagc
    10261 gaaaccaact taacagacaa gattgcaaaa gcaaatcaat taatcgacca acaaaaagca
    10321 aaattagaaa aactaaatgc ggttaaagct cgaaatgaaa aatattgggc aactgtcgaa
    10381 aaggtaaaaa ataccagcga acgtttgcat aatttaggac aacgctcaat gattagtggt
    10441 gcggcaattt ctgcccctgt tgttgggcta gggaaagggg ttgtcggaat ggctcaaaca
    10501 gcgggtaaat ttgaacaatt taatgctatt ttagaggtaa ccgagggaag tgctgaaaaa
    10561 gccaagcaaa gttttgattg ggttaaacaa tttgcagtag ataccccctc aaaccttgat
    10621 gaagcaatgg aagcctttgt aaagttaaga gcatacggtc ttgatcctac caatggctta
    10681 ctacaaacat taggtgatac aggtgctgca atgggtaagc ctgtaatgca agcagttgag
    10741 gcaatagcag atgctttaac gggtgagaat gaacgcttaa aagaatttgg tattaaagga
    10801 agtgtagtta aaggtacaaa tattattgag tatgcctaca ctgataaatt aggcaaacaa
    10861 caagtcgcaa aagtaaacaa aaataaccga aaagaaattg agcaaaccct aacccgtatt
    10921 tttaacgaaa aatatgctgg agcaatggaa aaacaagcta aaacgctagt tggtatttgg
    10981 gcaaaaattg aagattattg gacaaatttc caaatgcaag tgatggaatc gggagcattt
    11041 gactggatta aaaataaatt gcaatgggtg ttagacagtt tagataaaat ggctgaaaat
    11101 ggcgagttgc aaaaatgggc tgaagatgtc ggagccgtta ttcaagaaac agtacaaggc
    11161 ttatgggctt ttggtgaaaa aattattgaa gtggttaaat ggttagcgag cttggcacgg
    11221 gagaataaag ggctaattgc aacattcgtt aaatggtctg ctattcttgg ctctagttta
    11281 acggtgctag gagcttttgc agtggtggca agttttgcat tatatcctgt agcaagattg
    11341 gctcttggta tcggcaaatt tacaggtgca agcaccttat taaataaggc attatttgat
    11401 agtaatgata aattccgatt attcaataaa tcactttatt catcaggcac tacggctgct
    11461 tttgttaaat caaaatttaa tcagttagca actttaccaa atgtgctggg aagtagtttt
    11521 aataaattag gcggtggcat caaaatgcta attccacagc ttaaaacaaa agtattttgg
    11581 ctcaatatgc taaaaggtat tttaggcaag ttgtggagtg caatccgttt tattttctca
    11641 ccgttgaaat tattacttgg tattttctcg ccgattggct tagcgattac agctatttcg
    11701 gtagcagggg tagcgttata tagaaattgg gagaaggtca gagccttttt tggaggcttt
    11761 ttgcaaggat tatctaaagg tcttgcacca gtattagaga gatttaaacc tttaggaaaa
    11821 ctatttgaaa ctatatcaaa ctgggttaaa tctacgttta agtggttatc cgatttatta
    11881 accccagcgg ataaaacatc ggaaagttta gataaagcga gttctgcagg agaaaaattc
    11941 gggcttgcat tggctggtgc aatagacttg gtaacatcac ctgttcagtg gctagtcgat
    12001 cagtttaagt ggatttctga aaatatgccg agttgggaca ctattggtga gggtgtaaaa
    12061 aaggcagcta ctgcaattgt tcccaccgag cacgccaagc agattactaa aacggcagaa
    12121 atgtcaaaca atatgtatga ccccaattat actccaccac aacttgagca aaaatggatt
    12181 ggtggcttgg ttggtaacgg taaaggaaga ggttttgcca caggcggcta caccggcaat
    12241 ggtggcaaat atgagcctgc cggtattgtg cataagggcg agtatgtaat gaccaaagag
    12301 gcaactgcac gcttgggtgt ggcaaatctt aaccggttaa attatggcaa agttgctggg
    12361 ctaactgcac ttgcgtcaag tgttgcgttt gctcaaccaa tgcctgcggt caaaatcgac
    12421 agcagaccac cgctgacggc aagccaacca agcccaacag ttgcaccggt gtcgcaaaat
    12481 atccatatca ccatcaacgc caccggtggg caagacccac aagcaatcgc ccgactggtt
    12541 gcaatggagt tggaaaaaca acaacgccaa gcacaagcaa gagcaagatc gtctttgaga
    12601 gacaggggct aatttgacaa agtagaatga aaagcggaca atgctctaaa ttgtccgctt
    12661 tttataggag caaatgatgt ttaaagaaaa aggatatgat gaatttgtga atgaatgtgt
    12721 gcgaaaaggt agagaagaac acaaagctgg tttaggattt acgctagagc aatctcgctt
    12781 acatatcaaa aaaaccattg agcaaaaatc acaagagcta aacgaatttc agcgtgaagt
    12841 ggtttatggc taaaattacc attaaaccac aagcattgag tcaatttgac gagattgtaa
    12901 aaagtgttat cgaatttacc ggttttgaaa cgagtggtat tcgattacaa agtgatattt
    12961 ttgacaaaat agagcaaatt gcatttatgc cactctctgg aagagcaaga gaagatggga
    13021 caagagaaac tttcgtcaga cgttatcgta ttgtgtacga ttacgatagg caggcagatg
    13081 aagtaattat cttagctatc attcacagta gccgacttta cccacgccca taatcagtaa
    13141 gcgacctaat cggtcgcttt ttctttgcct gttaaaaccc atttcacacc tcgcatttat
    13201 acccaaagcc tttcgccttt tgcaaaatat cctcattttt taaccgcttg taattgtaaa
    13261 aatgagtgag ccaaaccacg ccgaacaaat ccgccgttta gacaatatta tccgacttgg
    13321 cactattgcc gaggtggatt taaccacagc gactgcacgg gtcaattcgg gcggtattac
    13381 gaccgatttc ttaccgtggc tgacttttcg tgccggtaca tcgcaaacgt ggtcagctcc
    13441 aacagttggc gagcaatgcg tggtgttaag cgtgagtggt gaatttacca caggcgtgat
    13501 cttatttggg atttacaccc aaaacgcccc gagccaatcc gccgatgagc atctattccg
    13561 cttttcggac ggtgcagaaa tcagctacaa caccgcaagc ggtcaattaa ccgtaaaaaa
    13621 ttgcaaagtg gtgattgtgc aggcgacaga atctatcacg ctcgacacgc ccacggttaa
    13681 ggccacaaaa aatgtcgtga ttgggggcaa cctatccgtg aaaggcacaa cctcatcgca
    13741 aggtgcaatc agcacacaag gggcggtaac agcaaaaggc gatgtatcag gggcgggaat
    13801 cagtttaggc agccaccatc acattgagca aggtgacggc agaccaacta gcggagcaaa
    13861 accataatga accgagaaac aggcgaacag cttgaagacg aagtagagca tatcaagcag
    13921 tctatcaaag atattttgct gacggcaaaa ggcagtcggg taatgcggcg aacctatggc
    13981 agtaatctct acaagctaac tgataaaccg attgccgcaa gtctgattat gcaactctcc
    14041 gctgcgtgcg tgatggcgtt gcagcaatgg gagccacgta tagcgatcac ccgatttaaa
    14101 gtggaatttg ccgaagataa ctacaataag ctgatttgca ccttagattt tacccttaag
    14161 caccgaaaaa tcagcatcaa aggaatgcgt tttacactat gagcgaaatt gtcgatttat
    14221 ccaaactccc cgccccgaaa gtactggaag aattggacta tgaaacctta ctggcagagc
    14281 ggaaagccaa gtttttatcc ctctacccag aaagcgaacg agcggtaatg gctgcacggt
    14341 tagcgttgga gtctgagcct atcaccaagt tactacaaga aaattgctat ttgcagctac
    14401 tcgaacgcca acgcattaat agtgcggctc aagccacaat gcttgcttat gctaccggta
    14461 cggatttaga tgtcatcgca gccaacttta atgttgagcg gcttgtagtg caagctgaag
    14521 acttaactgc aagcccgcct atcgtcgaag agttagagcc tgatgatgat ttccgtcagc
    14581 gtgtgcagtt aaaaattgaa agtgtttcgg ttgccggtcc acggaaggct tacacctttc
    14641 acgccctgtc tgctaacgga aaaattgccg atgtgtccgt catatcacca cagccagctt
    14701 atgtaacggt aacgctgctt tctcgtgatg gcaacggagc cgtagcctca gaaatcatca
    14761 atgcggccaa aattgctctc aatgatgaag aggttagacc gattgccgat agagtaaccg
    14821 tgcaaggggc aagcattgtg gaatatgaaa ttaatgcggt gttgcatctc taccgtggac
    14881 ctgagaaaga gcctatttta aaagcggcaa aagctaactt agaggcttac atcaaccaaa
    14941 caaaacgcat tggacgagat gtgactcact caggtatcca cgccgccttg cacgttgccg
    15001 gtgtgcaaaa tgtggaaatt ttgcagccga gaactgattt ggtgctgaca ccttaccaag
    15061 ccggttattg tgtcaattac acgctaaatg ttgaggtgag cgatgaatac agttaaagca
    15121 ttattgccac ccggttcatc acttttggaa aaacgagctg ccgaatgttt gcaacaagcg
    15181 gtacaaaatc cgattaaatt tgcaaatttg attaatccac acaaatgccc cgtgaaattt
    15241 ttgccctatc ttgcgtgggc cttatcggtg gattattggg aagagcattg gagtgaagaa
    15301 ttaaaaagag aaaccattat tcgatctttt gaagaacacc gaaagaaagg cacagcaagt
    15361 gcagtgcgaa gaattgtgga atctctcggc tatgcttttg aaatccgtga gtggtttgcg
    15421 gaaaaacggc aacggcagcc cgggacgttt agggtttttg ttgagttaaa agataaaggg
    15481 attagcgaaa aaatctacca agaattagaa aggttgattg aagatgcaaa accggtatca
    15541 aggcacatca cagaattagc agtatcagcc acctcaaaag gcaaagcaaa tatttttagt
    15601 ggagcacaca gtggcgaaat tattacgatt tatccaccta aaaagctccc tgttattgtc
    15661 attaaaagca aaatggattt atttagtgca acaaacagtg gcgaaattat tacgatttat
    15721 cctaataaga ggtaaaaatg gaacaaaaat ataaaacaat attaacccat cacggtgagc
    15781 gagtgatcgt tgaggctctt gcaaataaga taccagtgcc acttaaggaa atggctatag
    15841 gagatggtaa cgggtcatca ataactccaa gtgcatctca aaccacactt gtaagagaag
    15901 tttatcgtgc ggaaattaca gatttgctag aagacccgca aaatcgccat cagatgattg
    15961 ctgaattact aatcccagaa aatgtaggtg gatttattgt ccgagaaata gggttatttg
    16021 atgaacaagg tggtttggtt gctgtagcaa attgccctga aaattataag cctgtattag
    16081 aacaaggcag tggtaaagtc caatattacc gaatgatttt acaagttagt agtagtgatg
    16141 cggtcacctt atcgattaac aataatattg tttacgccac aagaactgag tttaacgaat
    16201 ttgtaaataa cttgagtagt cctgatggct ataaacacat cggacgctgc aaatctgtag
    16261 cagaactccg cactat
//
LOCUS       DANLXQ010000046        16231 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.046, whole genome shotgun sequence.
ACCESSION   DANLXQ010000046 DANLXQ010000000
VERSION     DANLXQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16231
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.046"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            86..1504
                     /gene="leuC"
                     /locus_tag="PXD94_001092"
     CDS             86..1504
                     /gene="leuC"
                     /locus_tag="PXD94_001092"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL6285970.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKADEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            1517..2119
                     /gene="leuD"
                     /locus_tag="PXD94_001093"
     CDS             1517..2119
                     /gene="leuD"
                     /locus_tag="PXD94_001093"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL6285971.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(2191..2655)
                     /locus_tag="PXD94_001094"
     CDS             complement(2191..2655)
                     /locus_tag="PXD94_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285972.1"
                     /translation="MNKFKIALMATVAIGLFACTPSPQSNSTKQQAQVGYLKQNISQA
                     ELNNPKDFKRYSYYCKNFATGSSSYLATYFPLWSESRKQENFGIYFQLDGGKVYPFDH
                     LANISLNARATKFEVRYRSYQPIEGSFVELVAREFTSTYYKNNVPWLECREG"
     gene            complement(2679..3185)
                     /locus_tag="PXD94_001095"
     CDS             complement(2679..3185)
                     /locus_tag="PXD94_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285973.1"
                     /translation="MADPHIQSPMDFWDYLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSVWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWRMPLHFDI
                     GDKSKYQI"
     gene            complement(3208..4185)
                     /gene="dusA"
                     /locus_tag="PXD94_001096"
     CDS             complement(3208..4185)
                     /gene="dusA"
                     /locus_tag="PXD94_001096"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL6285974.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKSGAGVEVVEQALAFIG"
     gene            4536..5513
                     /locus_tag="PXD94_001097"
     CDS             4536..5513
                     /locus_tag="PXD94_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL6285975.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYCYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPELLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            5500..7167
                     /locus_tag="PXD94_001098"
     CDS             5500..7167
                     /locus_tag="PXD94_001098"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL6285976.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            7171..7590
                     /locus_tag="PXD94_001099"
     CDS             7171..7590
                     /locus_tag="PXD94_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6285977.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(7825..8586)
                     /gene="nudC"
                     /locus_tag="PXD94_001100"
     CDS             complement(7825..8586)
                     /gene="nudC"
                     /locus_tag="PXD94_001100"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL6285978.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(8579..9262)
                     /locus_tag="PXD94_001101"
     CDS             complement(8579..9262)
                     /locus_tag="PXD94_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL6285979.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWNIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(9259..10107)
                     /locus_tag="PXD94_001102"
     CDS             complement(9259..10107)
                     /locus_tag="PXD94_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6285980.1"
                     /translation="MEKRTLALLKVHGTAVLFGASGIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDTINARTASMIISLEPVYAIVIAWLCFNEVPTLKMIIGGLIILLSVA
                     WANLKK"
     gene            complement(10107..10592)
                     /locus_tag="PXD94_001103"
     CDS             complement(10107..10592)
                     /locus_tag="PXD94_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL6285981.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSVTSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(10594..11916)
                     /gene="pepQ"
                     /locus_tag="PXD94_001104"
     CDS             complement(10594..11916)
                     /gene="pepQ"
                     /locus_tag="PXD94_001104"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDL6285982.1"
                     /translation="MKNLFSTHLARLQQTVQSLLENAFLDGLWIYAGKAKTLFLDDQT
                     APFKINPYFNYFFPYPQAENCWLFLDGKNKPKIYFYAPEDYWVSTLKVPTEAFFAAEF
                     EWVLLRAENEIATFIQNPTACAFIGEDVELAKKLGFSHINPQKVLNSLNFARSIKTEF
                     EIESIYQAQFSALKGHQVAKEAFFAGKSEFEINLEYLKATQQSDLNVPYGNIVAINQN
                     GAVLHYTALDFDTHAKRHSFLIDAGTTVNGYASDLTRTYAFDSGSEFAELVAKMEQLK
                     LQIIAELSIGVNYLHYHTRMQQMISQLLAEHQFIHLSAEQIFEQGISRTFFPHGLGHL
                     LGIQVHDVGGFQQNARGSHKAPPEVYPSLRCTRELQENMVLTIEPGFYFIEMLLKPWK
                     NSALSSAFNWQKIEAFKAFGGIRTEDNIVMRANGAENLTANAAATLGG"
     gene            11927..12169
                     /gene="tusA"
                     /locus_tag="PXD94_001105"
     CDS             11927..12169
                     /gene="tusA"
                     /locus_tag="PXD94_001105"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL6285983.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(12236..12667)
                     /gene="rraB"
                     /locus_tag="PXD94_001106"
     CDS             complement(12236..12667)
                     /gene="rraB"
                     /locus_tag="PXD94_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL6285984.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(12682..13293)
                     /locus_tag="PXD94_001107"
     CDS             complement(12682..13293)
                     /locus_tag="PXD94_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL6285985.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFKVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(13295..13711)
                     /locus_tag="PXD94_001108"
     CDS             complement(13295..13711)
                     /locus_tag="PXD94_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL6285986.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(13715..14545)
                     /locus_tag="PXD94_001109"
     CDS             complement(13715..14545)
                     /locus_tag="PXD94_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL6285987.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLVLPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVALG
                     "
     gene            complement(14882..14997)
                     /gene="rrf"
                     /locus_tag="PXD94_001110"
     rRNA            complement(14882..14997)
                     /gene="rrf"
                     /locus_tag="PXD94_001110"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(15159..>16231)
                     /locus_tag="PXD94_001111"
     rRNA            complement(15159..>16231)
                     /locus_tag="PXD94_001111"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         4950 a         3626 c         3168 g         4487 t
ORIGIN      
        1 atatcagcaa gcggtcattt ttgcaaaatt ttttgcaaat ttaaccgctt gtattaaaag
       61 ttaccctttt gagttggaac attttatgca acaagccaaa accctatatc aaaaactatt
      121 cgactcacac gttgtgtatg aagccgaagg cgaaacgccg attatttata ttaaccgcca
      181 cttaattcac gaagttacca gcccgcaagc gtttgacggc ttgcgtgttg ccggtcgcca
      241 agttcgccaa gtcagtaaaa ccttcggcac aatggaccac agtatttcaa cccaagtgcg
      301 tgatgtacag caacttgaag ggcaagcgaa aattcaagta ttagaattag aaaaaaactg
      361 cgaagaaacc ggtatcccac tgtttgacat cactaaaaaa caacaaggta tcgttcacgt
      421 aatggggccg gagcaaggct taactttacc cggtatgacc attgtatgtg gtgactcgca
      481 caccgccact cacggggcct ttggtgcgtt agccttcggt atcggtactt ccgaagtgga
      541 acacgtttta gccacccaaa ccttaaaaca ggcccgtgca aaaagtatga aagtggaagt
      601 ccgtggcaaa gtaaacccgg gcattaccgc aaaagatatt gtgcttgcga tcatcggtaa
      661 aacaactatg gctggcggta caggctatgt agtggaattt tgtggcgaag caattcgtga
      721 tctttcaatg gaagggcgaa tgacagtgtg taatatggcg attgagtttg gtgcgaaagc
      781 cggtattgtt gccccggatg aaaccacgtt cgcttacttg aaagaccgcc caaatgcacc
      841 aaaaggtcaa gattgggaag atgccattga atattggaaa accctcaaag ccgatgaggg
      901 tgcagtttac gatgcggaag tgatcttaga agcaaaagac attgctccac aagtgacttg
      961 gggaaccaac ccggggcagg tgatcggtat tgatgatgta attccaaatc cggaagatat
     1021 ggccgatcct gttactcgtg cctctgcgga aaaagcctta gcttacatcg gcttagaagc
     1081 ccatacagat atgaaagatg taccggttga tcaagtcttt attggttctt gtactaatgc
     1141 tcgtattgaa gatttgcgtg ctgcggcagc agtaatgaaa gggcgtaaaa aagccgacaa
     1201 tgtaaaacgt attctcgttg tgccaggttc aggtttggta aaagagcaag cagaaaaaga
     1261 gggcttggat aaaatcttca ttgaagccgg tgcagaatgg cgtaacccgg gttgctcaat
     1321 gtgtttggga atgaacgatg accgcttggg cgaatgggaa cgttgtgcct ctacgtctaa
     1381 ccgtaacttt gagggcagac aaggacgtaa cggtcgtacc cacttagtca gcccggcaat
     1441 ggcggctgcg gcggcggtgt tcggtaaatt tatggatatt cgcaaagtgt cattaaacgc
     1501 ataataggag gaaaaaatgg caggtattaa acaacattca ggcttggtcg ttcctcttga
     1561 tgcagcgaac gtagacaccg atgcaatcat tccaaaacaa tttttgcaag ccattactcg
     1621 agtcggcttt ggtaagcacc ttttccacga atggcgttat ttagatgcgg aagagactca
     1681 aatcaacccc gatttcgtac tgaacttccc acaatatcaa ggtgcaacca ttttactcgc
     1741 ccgtaaaaat ttaggctgtg gctcttctcg tgagcacgcc ccttgggcat tggcagatta
     1801 cggttttaaa gtaatgatcg ccccaagttt tgcggatatt ttctacaata acagcttaaa
     1861 taaccatatg ttgccgatta aattaaccga agaggaagtc gaagagatct tccaatgggt
     1921 gtgggcaaat gaaggaaaac agatccacgt tgatttagaa gcgatgactg tgactgttgg
     1981 cgagaaagtt tatacctttg agttagacga attccgccgc cactgcttat taaacggttt
     2041 ggataatatc ggcttaaccc tacaacacga agctgcgatt gctgcttatg agaaaaacat
     2101 tccggcattt ttacgctaat taaaatacaa aaaaacggct aacatcttta aggcgttagt
     2161 cgttttcttt ttagccattt tctttggcaa ttagccttct cggcactcta accaaggcac
     2221 attatttttg taataagttg aagtaaactc tcgtgccact aactctacaa aactgccttc
     2281 aatcggctga taagaacgat aacgcacttc aaattttgtt gctcttgcat tcaaactgat
     2341 gttggcaaga tggtcgaacg gataaacctt tccgccatca agttgaaaat agataccaaa
     2401 attctcttgc ttacggcttt cagaccaaag tggaaaatag gttgccaaat atgaactgct
     2461 gcctgttgca aaattcttac aataatagct ataacgctta aaatctttcg gattattcaa
     2521 ttccgcttgg ctgatattct gtttcaaata tccaacctgt gcttgttgct tagttgagtt
     2581 tgattgtggt gagggcgtac aagcaaataa gccaattgcc actgttgcca ttaaagcaat
     2641 tttgaattta ttcataaaaa tcccgataaa atattaaatt aaatctgata tttgctttta
     2701 tcgccaatat caaaatgcag cggcattcgc catttttgaa tactcagcac aagaagcaac
     2761 aagccacaga caaggcttaa aatttgcacc aataaagacc attctagtgc aaaagcaatc
     2821 cacaataccg ccactaaaag cggttggaaa tttaagccga atactctaaa acagaaatat
     2881 tctttatagc ttaatccgcc gatcaccagt agcattgccc cgaacgcaag caaagggaaa
     2941 ttaaacacag caccaagtaa gccaatccaa acagaaaatt ggaaaatcag gcgaaacgat
     3001 ttggcataaa gatgcaaaga agatgccaac attgtgccag atactaataa accatattgt
     3061 gccgtttgcg gataataagg caataaaagc aacatcaatg tagctaacac aaaaccgctg
     3121 cggtaaatga tgacggttaa ataatcccaa aaatccattg gcgattgaat atgtggatct
     3181 gccataactg cctccttaaa caataaatta gccaataaac gcaagggctt gttccactac
     3241 ttctacaccg gctcccgact tagtggcatt ttcacttaaa tgacgacgcc actgcctcgc
     3301 tcctttgcaa tgttgaaacg caccaagcat atggcgaacg atatgattta aatagacacc
     3361 tttggcaagc tcacgttcaa tataagggaa catcttttcc accgcttctt ttgccgttac
     3421 aagcggtcga ttttcattaa aaatttgcga atcgatctcg cctagcattg acgggttttg
     3481 ataggcctca cgccctacca ttaccccatc aacaaactgc aaatgctgtt taatttcctc
     3541 aatggtttta atgccgccat tgatgctaat ctttaaatgc ggaaaatctc gcttgagctg
     3601 ataaacccga tcataatcta aaggcggaat ttcacggttt tccttcgggc ttaaccctgt
     3661 cagccacgcc ttgcgggcgt gtacgataaa atcattgcta taaggttgca ctttttcgat
     3721 aaaatcacac aaaaattcat agctgtctaa atcatcaatc ccaatacgat gtttaaccgt
     3781 gaccggaatt tccaccgcat cttgcattgc tttaatacaa tccgccacta aatctgcttt
     3841 tgccatcaaa caagccccaa acataccgtt ttgcactcgg tctgacgggc aaccgacatt
     3901 tagattaatc tcggcatagc ctcgctctgc caccaatttg gcacaatgag ccaactgtgc
     3961 cgggtcgctt ccacccagtt gcaaagccac cggattctcg ctcggatcat attccaacaa
     4021 atcatatttg gcgtgaataa tcgccggggc ggtgatcatt tcggtataaa gcaaggcttg
     4081 acgactaaat tggcgatgaa aatagcggca gtgacgggtt gtccaatcta acattggagc
     4141 aaccgaaaaa cgtccacggt aaaaaggttg aatttcgtta gtcataaaaa tatcaaaata
     4201 aagcggtaaa aaatcagtaa aaaataaccg cttgtaagcg attcaaaaca agcagccatc
     4261 atactcgaaa aagccagata aacatagttt cctcaaggct tccccatcta tctgcgcact
     4321 tttaaaacaa ccttaaaaca caatatatag tgtttcaaca tcaaatcaca cacaacatat
     4381 tgaagcaaaa atttacaaaa ttatttttct ttataaatca ataagctacc aaagaccaat
     4441 aaaaaagagt gtttgtcaag acttgtcttg aaactttttt tgcatacaat catctaccag
     4501 ttccaaacat aaactttaag atagagccta cgaatatgtc tagaaacctc ttccaaaaac
     4561 gcttaaatat taagccgtat gagtatcctg agcttcttga atttaaagat gcaattcgcc
     4621 attcttactg gttacacacc gaatttaact tcaccggcga tattcaagac tatcgcacca
     4681 ccattaacga tcacgaacgt cacgtgttaa cccgagcaat gcttgcgatt tcacaagtag
     4741 aagtgaatgt aaaacgtttc tggggcgagc tatactgcta tttcccaaaa ccggagatgg
     4801 acgatgtagg cggtactttt gctgaaagtg aagttcgcca taaagatgct tactcgttcc
     4861 tattggaaaa attaggccta aatgaaatgt tcagtcaaat tcatgaaatt gaaccattaa
     4921 tgaaccgcat tcgttatatg gaaagtttta tgaaagataa agatgctggc aaaggcgaat
     4981 ttgttttatc gttagtgctg ttctctttat ttgttgagca tatttcattg tttggacagt
     5041 ttattatcat gatgtcgttc aacaagcata aaaacctgtt taaagggatt tccaatgcgg
     5101 ttgaagccac ctcaaaagag gaagaaattc acggtaaatt cggaattgcg ttatacgaaa
     5161 ttttacgtga cgaacacagt gaactattca caccagaatt ttttgaagat ttgcaaacct
     5221 tggcaaatca agcgtttgaa gcagaacgtg gcatcttaga ctggattttc gaagaaggtg
     5281 atttaagctt tatcaccaaa gcgacggtag aaaattacat taaaaaccgt tacaacaatt
     5341 ctttaagcat tttagggtta gagccgccac acgacatcga ccctgaatta ttaaaagaga
     5401 ctgagtggtt tgatattgaa attctttcaa ccaaagaaac cgatttcttt aacaaacgta
     5461 gcaccgatta ttctaaaaaa atgaaacaga ttaccgcaga tgacttattt taatacagac
     5521 cgcccggact tccactggct taatgaagac agccgtttat tcttacaacg tggctattta
     5581 ttggaaggaa caaccgcatt agaccgcatt cgttttattg ccgaacacgc agagcgaaaa
     5641 ctcggcattg aaggctatgc cgataaattc tatcactata tggcaagagg ctacttctcg
     5701 ctttcttcgc caatttggtc aaactttggc ttagatcgtg gcttaccgat ttcttgtttc
     5761 ggtagctaca tcggcgactc tatccatgaa attatggtga ctaccgctga agtgggcatg
     5821 atgagtaaaa ttggcggtgg tacttcggcc tactttggcg atattcgtcc gcgtggctcg
     5881 ttaattaaaa acaacggtaa atcagacggc tcatttaact tcagtaaatt atttgacact
     5941 gtgattgacg tgatttcaca aggaacatca cgcaaaggtc agtttgccgg ttatattgat
     6001 attgagcacg gcgatattga cgaatggctg gatattcaca ccgaaggcaa cccaattcaa
     6061 ttaatgtatt acggtgtgtg tgtcggtcac gattggttag agtcaatgaa agccggcgat
     6121 ccatacaaac gtcagctttg ggcaaaattg ttacaacgta aaaccgaaac cggtatccct
     6181 tatttattct ttaaagacaa tgccaacgca ggtcgtcctg atgtatataa agataaaaat
     6241 atgacgattc acgcctctaa cttatgtact gaaattatgt tgccttccag caacgaagaa
     6301 agtttcgtat gctgtttatc gtctatgaac ctactctact ttgatgagtg gaaagatacc
     6361 gatgcaccag aaacattaac ctatttcctc gatgttgtaa tgagtgaatt tattgagaaa
     6421 agtcaatcaa ttccattctt agaccgtgca aaccgctttg cccgtcgcca tcgtgcatta
     6481 ggtttaggcg tgttgggctg gcacagctat ctacaggcaa ataatattgc ctttgacagc
     6541 ttccaagcga tgcaaaaaaa caacgagatt ttcaaaacgc ttcaagcaaa aaccttgaaa
     6601 gcctcgcaag aattggctca acgcttcggc gagccggaaa ttttagaggg ctatggtcgc
     6661 cgtaatacta cattaatgag tattgctccg actaaatcca gtagctttat tttaggcagt
     6721 gtgtcgccat cggtagagcc gtttaaatcc aactactatg taaaagattt agcgaagatc
     6781 aaaaccgtgt ataaaaaccc gttcttagag aaattactcc aagaaaaagg gctagacacc
     6841 gaagaaattt ggacgtctat tttacaaaat gatggctcgg ttcagcattt ggaacagctt
     6901 tccgagcacg aaaaagaagt atttaaaacc tttgccgaaa tcagccaatt aagcgtgatt
     6961 cagcaagctg ctcaacgtca aaaatatatt gaccaagggc aaagtattaa tgtgatgatc
     7021 cacccagcca caccggctcg tgatcttaac caattatact taacagcaga agagctgggc
     7081 ttaaaatcca tctactacca aaactcaatg agtgcagcac aggtatttaa ccgtaaccta
     7141 ttaagctgct caagttgcga agggtaaata atgggaaatt tatcgcatac ttcacctaat
     7201 actattgttg aagttgttgg tgaatttcct gaaataacat ctagtgggta tacctttgat
     7261 aaaggtccta ttcaattaag aatcggcaaa catactgctc caaataaagg ctttggtgta
     7321 aagcatattg aagccgagca tatgcaagaa attcttaatc taggatttac atcagttaat
     7381 gaatttgtca aagctatcat agtagaaaac gctaaaattt tttgcgaatt ctcagattta
     7441 aaaggcaatc atcgccctat tattctgaaa gcaagtattg gcattgtggt tctagagcgt
     7501 aagtttttgg aaagcggtac gatatattca gttgtaaccg cttatagaag aacaaatcct
     7561 cacggaatac aaataggcac tatgaaataa agtacgttag tgttataatc agttccacgc
     7621 aagagcattt gtagaaaata acaggcggat ttggttttct actgtgggcg gcaggtttcc
     7681 gctaacctgt aatagctatt taatttttat attaaatcct gaacttgaaa cagactaagg
     7741 gattgctgcg gtagctaaga atacaaacct ctctataatc atatagagag gttttttctt
     7801 attgatgagc acaagttaag ataattattt actctcccga cataacttca aggtagcttg
     7861 aatcagctca aaagctattg tgccttgttg tgggatttca ggtaagcttt cctcacagtc
     7921 gaaccatttt gcatcgcaga tttcttcttc ctgtagctga atttcaccgc tttcataatc
     7981 cgctaaaaaa cctagcatta atgaattggg aaacgcccaa ggttggcttc caaaataacg
     8041 cacatttttc actttaatgc ccgtttcttc aaacacttca cgatgcaccg cagtttctac
     8101 cgcctcaccg gcttcgacaa aaccggctaa agtggtataa attgtgcctt tatggcgtag
     8161 atgattggct agtaggattt gtctgccttt tcgcaccgcc acaataattg acgggctaat
     8221 cgttgggtaa gctcgattgc cacacgcatt gcagtaaatt gcaatttcat cgcttgccag
     8281 tatggttttc tcgccacatt ttccgcaaaa ttggtgagtc tgaaagaaat ggttaagcga
     8341 aacagctcga tgcaacaaat gagacagttc gggtggtcgg ttaatttggc ttcttaaata
     8401 agcaaactct cgctgattat ccgattctaa cgccttcacc aaataagccg gcgagccttg
     8461 atattgcccg atggttaatt tctcacgtcc acttaagccc agctcttccg cacttccgaa
     8521 tggaatttcc ccagcctcca aggcaatatc aaaatcgctg acgataatcc aatatccttt
     8581 attcatcttt caatcctaat tgtgtaaaca acttacgttt ttcattttca gatagcactt
     8641 gaaactcacc acagcctaaa ttgcctaatc taaactggcc tatcccaaca cgaattaacc
     8701 gtaaagtcgg gaaaccgata tgtgcagtca ttcttctgac ttggcgattt ttcccttcag
     8761 aaataatcaa ttctatccag ctggtcggaa tggttttccg ctcacgaatt tgcggggcga
     8821 tattccaagc aaaatcgggc gttgctattg ctctgacttt cgcgggcttg gtcaaaccat
     8881 cttttaactc cacccctttt gctaatttgg caagatcttc cggttgtgga acaccctcca
     8941 cttgtaccca ataggttttc tgcttgtcaa atttcgggtg agctaaacgg tgctggattt
     9001 cgccattatt agtcaaaagc aacaatcctt cactgtctct atctagccgc ccgaccgggt
     9061 aaatattggg catcgtaata aaatctttta gcgtttttcg cccctgttca tcggtaaatt
     9121 gggttagtac atcataaggc ttattgagta acacaagggt ggtttccgca aaggtaagcg
     9181 gtcgtttttg cggatttttt tgcaaatttt tagcgaaatt tgaccgcttg tttgtcattc
     9241 ttttaaaggt tggttgtttc attttttcag gttcgcccac gcaacagaaa gtaaaataat
     9301 taagccaccg ataatcattt taagggttgg cacttcatta aagcaaagcc acgcgatcac
     9361 aatcgcatac accggctcga gggaaataat cattgaggca gtacgggcgt tgatggtatc
     9421 aaggcttgaa ataaacagcg tataggcaag ggttgtacat aaaaaaccga tacagaaaat
     9481 ccaaaaccaa tcttgggtgg aaacgcttgg aaattcagtg atggagagag gcaacagaaa
     9541 aagtgtagca aaaatatact gccaccagct tgcttgcaca ccggaaattt tgctcatggt
     9601 acggcgattc gctaccgcca aaataccata caccaaagca gataacacgc cccacaataa
     9661 accttgagtg gcttgattac caaaagtaaa ttcaggggta actaaaatta aaccaaccgt
     9721 gatggcaatt aatagcacca cttcacgaaa gcgtaatttt tcacggaaga acaggctttc
     9781 aaataacgcc acaaatgccg gaaagctggc aaagcctaag gttgctaccg ccacaccacc
     9841 tactttaacc gccacaaaaa aagtaaccca gtgggcagct aataaagctg cagaaaatgc
     9901 aagttgagcc atttgctgta agttgagctt tacaagcggt tgttttttcc ataaaaaata
     9961 caaagagatg atagcaaaag caatcatcac tcgcccaagc acaatggttt cagccgagct
    10021 ttgaattaat gctccgaaga tccccgaggc accaaataaa accgctgtgc cgtgtacttt
    10081 aagtagtgca agtgttcttt tctccactat ttgatcctat tatgttgatc ttgctcgtcc
    10141 ggcttgcgtt caaattctgc atcaaacgtg ttgctatctt gcgcagatgt aacagaaaaa
    10201 gacgaagttt gggcggtaaa atgcataaac accttattct ttacgctatt aattacgaag
    10261 gcttgaatcc attttcggct aaagggtaac acgcataaaa tcgctaaaat atcacttaaa
    10321 aagccgggaa taatcagcaa aatgccggct acaataaaca tcaccgagtt gccgactgct
    10381 tcagtcggta tcttgccttg gcttaaatct tgcttgatgg aaaagagggt ataaacgccc
    10441 cttaacttca caatccataa ccctaaaaat gaaatcacga ccagtaacaa aatcgtgggt
    10501 aacacgccaa tgatcgagcc aatattcacc agcagcgaaa tttcgcaata gatataaaag
    10561 aaaataccaa acagaatcgc taaaataggc atattatcct cccaacgttg cggcagcgtt
    10621 tgccgtcaga ttttctgccc cattggctcg catgacaata ttgtcttcgg tacgaatccc
    10681 gccaaaggct ttgaaagcct caattttctg ccagttaaag gcagaagata aggcactgtt
    10741 tttccaaggc ttaagtagca tctcaataaa ataaaaaccc ggctcaatgg taagcaccat
    10801 attttcctgt agctcacggg tacaacgcaa acttgggtaa acctctggag gagctttatg
    10861 gctgcctcgg gcattttgct gaaaaccgcc cacatcgtgc acttgaatac ccaataaatg
    10921 ccctagtccg tgtgggaaga acgtacggct aatcccttgt tcaaaaattt gctcggctga
    10981 aagatgaata aattgatgtt ccgctaataa ttgcgaaatc atctgctgca ttcgggtgtg
    11041 ataatgcagg tagttcaccc caatactcag ctcagcaata atctgcaact tcaactgttc
    11101 cattttcgcc accagctcgg caaattcaga accgctgtca aaggcatagg tgcgggtgag
    11161 atcggaggca taaccattta ccgttgtgcc ggcatcaatt aaaaaactgt ggcgttttgc
    11221 gtgggtgtca aaatctaacg ccgtataatg tagcaccgca ccattttgat taatcgccac
    11281 aatattgcca taaggaacat tgagatcaga ttgctgcgtc gctttcaaat attcaaggtt
    11341 gatttcaaat tcgcttttgc cggcaaaaaa tgcctctttt gctacttgat gcccttttaa
    11401 ggctgaaaat tgggcttggt aaatactttc aatttcaaac tccgttttga tggagcgggc
    11461 aaaattcagg ctattcagca ctttctgcgg attaatatgg ctaaagccta actttttggc
    11521 tagctcaaca tcttcaccga taaaggcaca agcggtcgga ttttggataa aggttgcaat
    11581 ttcattctct gctctcaaca aaacccactc aaattccgca gcgaaaaagg cctccgttgg
    11641 cacttttaga gtggaaaccc aataatcttc aggggcataa aaatagattt tcggcttatt
    11701 tttgccatct aaaaataacc agcaattttc agcttgggga tatgggaaaa agtaattaaa
    11761 ataagggtta attttaaacg gggcggtttg atcgtctaaa aataaggttt ttgccttgcc
    11821 ggcataaatc cataaaccgt ctaaaaaggc attttccagc aagctttgca ctgtctgttg
    11881 caaacgtgcg agatgcgtag agaatagatt tttcataagg aataaaatga ctcaattaac
    11941 tgtaaataaa gaattagata ccttggggct acgttgccca gagccggtga tgctcacccg
    12001 taaaacaatc cgtaatatgg aagagggaga aattttgcat atcatcgccg atgacccggc
    12061 cactacccgt gatattccga gtttctgcga atttatggat caccaattaa ttcaaaaaga
    12121 ggtggactcc accccatacc attactggat taaaaaaggc ttggaataaa ccacaagcgg
    12181 tcgattttag ccataaaatt gcaaatttat gcacaaaatc gaccgcttgt tttaattaat
    12241 gacggcgagg ttcgtcctca aaatcgtcat tttcatcatc gaaaaattca ccgtcatcgc
    12301 cgtactcatc ttcatcagca ttcggatctt caaagtaggt tccccagccc tcatactccg
    12361 caccggcttt ttcaattaat ggcagtaact ctttttgttg tgcggtaatg agatccgcat
    12421 tcaagtgaat ttcgctcaca atgtcgcata caaaaatcac tttaccattt tcgtcttcca
    12481 cttcttccgc ttcggaaatt tcatagccca gcttataggc atcaactact aatttttcca
    12541 atttatcaaa atcgtggtga gcaacgtggt gttcaataat ataaagggct tcagggtctg
    12601 aaccatcggc taataatgat gaaatggttt catcggtttc tgtgcgtaat tcatcgagat
    12661 tgtacatatt gaattcctta cttattctgt gattaatttt ggtgcaaacc agctatctaa
    12721 tttgttcgac agcttatcaa taccgatttt attcagtgcg gaataagcct ctacttggat
    12781 atcaccttgg aatggtaaaa tcgcttctct taccattttt acctgcttag agcgtgccga
    12841 ttggcttaac ttatccgctt tggtcaacag cagtaacacc ggcaagttgg ctgacactgc
    12901 ccattcaatc atctgttgat ctaaatcttt caacgggtgg cgaatatcca ttaaaatcac
    12961 cacaccgcct aaacattcac gcttttgtaa atactcgcct aatgcttttt gccactggat
    13021 tttcatctgt tcaggtactg ctgcatagcc atagccaggt aaatccacta atttacaatt
    13081 cgtttcaacc ttaaacaagt taatcaactg cgttcgcccc ggtgttttgg aagttcgggc
    13141 taaacttttt tggttagtta aagcgttcaa agcagtcgat ttaccggcat ttgaacgccc
    13201 ggcaaaagca atctccacac ctgtatcttc aggtaaatga cgaatatccg gtgctgaagt
    13261 taaaaaatgg gttttatgat agtttaattt cattttattc tctaaattta aaagataagc
    13321 cttgctcgcc atattgattc ggttcaggat taccggcaaa tacaccccat tctaaaaaga
    13381 gcaacattcc aataaatgcc ggcatcacct gtccaagcaa tatctgcata attccttgtg
    13441 aataaggagc tgcaagataa caaataatcg gcactaatgc cattaacagg gcttttgccg
    13501 aacgatttcg gtcgtgtaat cgtttaataa taatagcaga caggctaaat cctgtaatta
    13561 taagcggtag cacactcaat aaagacaatt cagccaaatt tatccacata gcaaccataa
    13621 acaggaaaag aaagtggatt ccaaatccaa tccaaaaacc tcgtctattt agcctaccgt
    13681 taaaactaaa aagtgcttta taccaactca ttttctaccc taaagctaca tcaagtacca
    13741 tcattactac aaagcctaac atcaggctta aagtcgcaat atcagtattt cccgaacttt
    13801 gcgattccgg aatcagttct tctacgacca caaaaatcat cgctccggca gcaaaggcaa
    13861 gagcataagg taaaatagcc gtcatcgaca gcacaaaaac cgccccaatc accgccgcaa
    13921 taggctcaac cactgctgac atagagccat accaaaatgc tttcttccga ctatgccctt
    13981 cgcctcgaat tggcaataca agtgacgagc cttcgggaat gttttgtaac cctattccga
    14041 ttgccaaacc gatagctccc ataatggaag catctacgcc tgaaacttgg gttgccaaag
    14101 caccgaaagc cacccctatg gctaatcctt ctggaatatt atgaatggta atcgcaagaa
    14161 aaagcaaagt agattttgat aagccttgtt taagttgatc atttccttca gcttgctcaa
    14221 tcggcttact caagtgcaaa tgcggcacaa catagtcaat taatcgaata aaagctccac
    14281 cggcaagaaa gccaatcgct gcaggcagcc aagctaaaga gccataatcc gcctcggcat
    14341 attctaaaga aggtgcaagc aaagaccaaa acgaggctgc aatcattaca cctgcggcaa
    14401 agcccatcat actgtctaat aattttcgat taaccgattt aaaaaaatag acaaatgacg
    14461 aaccgaaaat cgtacaaccc caagtaaata aacccgctaa aaaagcttgc catattggag
    14521 aaagtgaaag gaaatattca aacaagaaat agtaccagct aaaataaata ttggctcatt
    14581 ctatcaaaga ttcagggtta ttaatatagt gtagccaaag gctcaacgaa gatttcactt
    14641 gtaaaactta gcaagaaaat aaccgcttgc atttcccttt cataaataca atgaaaatcc
    14701 taacaagcgg tcattttccg tgagtttttt acctttacca caacaacaaa aaaccgcctg
    14761 tatctctaca ggcggctctt tcttggatta cttgtacttt gtttatttgt acttattttg
    14821 cttttgctta agttttcgct tttgtaactt ttagctttcg cttaagctcg cttaaattaa
    14881 aacccgacga tgctctactc tcacatggcg aatcaccaca ctaccatcga cgttactgcg
    14941 ttttacttct gagttcggta tggggtcagg taggaccaca gcactcttgt cgtcgggaaa
    15001 attcttttct atttttgaat ttctttaagt taacttccta atctaactct tagttcactt
    15061 taatagttag attaacttcc ttaacttcga ttgatttctt ctctagtttc gttcttcttc
    15121 tcaatacaaa aacaagccta aaaacatttg agcgttgtat agttaagcct ctcgggcaat
    15181 tagtactggt tagctcaatg tctcacaaca cttacacacc cagcctatct acgtcgtagt
    15241 ctccaacaac ccttacacac ttatagtgtg ggagaactca tctcgaggca agtttcgtgc
    15301 ttagatgctt tcagcactta tctcttccgc atgtagctac ccagcaatgc ctctggcgag
    15361 acaactggaa caccagtgat gcgtccactc cggtcctctc gtactaggag cagcccctct
    15421 caattctcca acgcccacgg cagataggga ccgaactgtc tcacgacgtt ctaaacccag
    15481 ctcgcgtacc actttaaatg gcgaacagcc atacccttgg gacctacttc agccccagga
    15541 tgtgatgagc cgacatcgag gtgccaaaca ccgccgtcga tatgaactct tgggcggtat
    15601 cagcctgtta tccccggagt accttttatc cgttgagcga tggcccttcc attcagaacc
    15661 accggatcac tatgacctgc tttcgcacct gctcgacttg tctgtctcgc agttaagctt
    15721 gcttatacca ttgcactaac ctcacgatgt ccgaccgtga ttagcaaacc ttcgtgctcc
    15781 tccgttactc tttgggagga gaccgcccca gtcaaactac ccaccagaca ctgtccgaga
    15841 ccgcgttccg caatcttcgt tagaacatca aacgttaaag ggtggtattt caaggacgcc
    15901 tccacaatca ctggcgtgac tgcttcaaag gctcccacct atcctacaca tcaaaattca
    15961 atgttcagtg tcaagctata gtaaaggttc acggggtctt tccgtctagc cgcgggtaca
    16021 ccgcatcttc acggcgattt caatttcact gagtctcggg tggagacagc ctggccatca
    16081 ttatgccatt cgtgcaggtc ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt
    16141 tatagttacg gccgccgttt actggggctt cgatcaggag cttctcttgc gataacacca
    16201 tcaattaacc ttccagcacc gggcaggcat c
//
LOCUS       DANLXQ010000047        16058 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.047, whole genome shotgun sequence.
ACCESSION   DANLXQ010000047 DANLXQ010000000
VERSION     DANLXQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16058
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.047"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            106..2328
                     /gene="glgB"
                     /locus_tag="PXD94_001112"
     CDS             106..2328
                     /gene="glgB"
                     /locus_tag="PXD94_001112"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL6285988.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDSTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGECYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFSYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNHLYTQTPALFEQD
                     YNPEGFEWLVVDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTKQNKT
                     "
     gene            2352..4364
                     /gene="glgX"
                     /locus_tag="PXD94_001113"
     CDS             2352..4364
                     /gene="glgX"
                     /locus_tag="PXD94_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL6285989.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLAHPVSIDYLKTLGITAVELLPVTYHIDETHLQKQGL
                     VNYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKN
                     GPMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDG
                     FRFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGVEGYQLGQFPEPFY
                     QWNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFTKYSVSKSINFITAHDGFTLQDL
                     TSYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAG
                     VPMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSK
                     QNNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQG
                     NWKVVLEQAQGILMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            4357..5670
                     /gene="glgC"
                     /locus_tag="PXD94_001114"
     CDS             4357..5670
                     /gene="glgC"
                     /locus_tag="PXD94_001114"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL6285990.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRPKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRVHVEAESVIEHTVILPQVTIGKNCVIKRAVIDRHCVIPDG
                     LEIGVNAEEDAKRFRVSKNGIVLVTQSMLDKLAGKEANSDIKYTI"
     gene            5793..7229
                     /gene="glgA"
                     /locus_tag="PXD94_001115"
     CDS             5793..7229
                     /gene="glgA"
                     /locus_tag="PXD94_001115"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL6285991.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAGAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLANIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
     gene            7324..9824
                     /locus_tag="PXD94_001116"
                     /pseudo
     CDS             7324..9824
                     /locus_tag="PXD94_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
     gene            complement(9906..10622)
                     /gene="trmJ"
                     /locus_tag="PXD94_001117"
     CDS             complement(9906..10622)
                     /gene="trmJ"
                     /locus_tag="PXD94_001117"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL6285992.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRTNLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
     gene            10802..12340
                     /gene="pntA"
                     /locus_tag="PXD94_001118"
     CDS             10802..12340
                     /gene="pntA"
                     /locus_tag="PXD94_001118"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL6285993.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNTFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDNQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQLQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            12355..13800
                     /gene="pntB"
                     /locus_tag="PXD94_001119"
     CDS             12355..13800
                     /gene="pntB"
                     /locus_tag="PXD94_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL6285994.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEHRETKAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(13867..14793)
                     /locus_tag="PXD94_001120"
     CDS             complement(13867..14793)
                     /locus_tag="PXD94_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6285995.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(14903..16018)
                     /gene="asd"
                     /locus_tag="PXD94_001121"
     CDS             complement(14903..16018)
                     /gene="asd"
                     /locus_tag="PXD94_001121"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL6285996.1"
                     /translation="MQNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKA
                     PVFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASAL
                     RMKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVA
                     TYQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILDIERKVTAKMRAEDFPTEN
                     FGAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRC
                     HSQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGR
                     LRKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
BASE COUNT         4602 a         3109 c         3672 g         4675 t
ORIGIN      
        1 atttagctca aaaaatggcg taaatccact tcttttattt ctatttagtg atagagtgag
       61 aactattgaa atttaagtat taaacgcttt aagcaaggag tgccgatgca agactatact
      121 tattctgaaa aagatgcttt ttttgttgag cgactttctt cgggttcttg taatgatcct
      181 ttctcctacc taggtattca ccctagcaat aacggcgtta ttattagggt atttcttcct
      241 tctgccatag cttgccaagt gatcgattta aacggaaagc ctgtgtcatc aatgctaaaa
      301 attgatgata gaggcttttt tattgcagag ctgattggta aaaaaacaga tttttcttac
      361 cgcttgttgg tggaataccc tgattcaacg gtggatatag aagaccctta ccgttttaaa
      421 acccatattc aagagcttga taattggttg tttgcggaag gaaaacaact tcgcccgtac
      481 gaaaaattag gcgcacattt agtcacacaa aatgaagttg ggggtgtgca ttttagcgtc
      541 tgggcgccga acgctcagcg ggtttccgtg gttggggatt tcaaccactg gaacggcttg
      601 gtacattcaa tgcgattcca ccccgattcc ggtatttggg atattttcct gccgaatgtg
      661 aaaaaagggg agtgctataa atttgaagtg ttggatcgaa acggcgtgct acggttgaaa
      721 tccgaccctt acgcctttgc ctcccaattc cgcccagaaa ccgcatctat tgtttccggc
      781 ttgccaagca ttgtgccagc cgagaaaacc tgccgtaatg cgaatgctcc cgataaaccg
      841 atttccattt atgaagtgca tttaggctct tggcgacgca acattgaaaa caattattgg
      901 cttagctatg aagagcttgc cgatgaactc attccttatg tgaaagatat gggctttacc
      961 catattgaat tgctgcctat ttccgaattt ccgtttgatg gctcttgggg ttatcaaacc
     1021 accggtattt attcgccaac cagccgtttt ggtacgcctg agggcttgag ttactttatc
     1081 caaaaagctc acgataaagg cattggtgtg attcttgact gggtagtggg gcattttccg
     1141 gtagatgaac acggcttggt ttattttgat ggaacgcatt tatacgaaca ttccgatccg
     1201 aaagagggct accacaaaga ttggaatacc ttaattttta gctacggcag aaatgaggtg
     1261 agcaattatt tgtctggtaa tgcactttat tggattgagc gttttgggat tgatgcgttg
     1321 cgggtggatg cggtggcgtc aatgatttat cgtgattact cacgttcaga cggcgagtgg
     1381 attccaaata aatatggcgg acgtgagaac ttagaggcaa tcgacttctt acgccacacc
     1441 aataatattt tgagagacga aagccaaggc ggtgcgagca ttgcagagga gtccacttct
     1501 tttgccggag tgtctcactc aactgaaaga ggcggtttgg gctttaatta taaatggaat
     1561 atgggctgga tgaacgacac tttacgctat atgcaaaaag atccaattta ccgccgttat
     1621 caccacaatt taatgacttt tggtatgacc taccaataca gcgaacagtt tgtgttaccg
     1681 ctttcacacg atgaagttgt tcacggcaaa ggttcattgc tgaataaaat gccgggggat
     1741 tgctggcaaa aatttgcgaa cttacgagcc tattatggct atatgtgggg ctatccgggc
     1801 aaaaaattgc tctttatggg caacgagttt gctcaaggta aagagtggaa ttttgccgag
     1861 agcttagatt ggtatttact cggggaagaa gggggcgaat ggcataaggg cgtattgggt
     1921 tgggtaaaag cccttaacca tttatacacc caaacccctg cactgtttga gcaagattac
     1981 aatcctgaag gttttgagtg gttagtggtg gacgatcacg ataattcggt gtttgctttt
     2041 gagcgtaaag ccaaaaatgg cgattcggtg attgtggtga gtaatttcac cccgattgtg
     2101 cgtgaaaatt accgttttgg ggtgaatgaa ttggcagaat atcaggaaat tctcaattct
     2161 gatttagctt gttattgcgg tagtaatgtc agcaatgcaa accgatttgc cagcgagtta
     2221 attgcatcgc acggtaaagc ctactccttg agcattactt tgccaccgct atcgactatc
     2281 ttcctcaaaa gagccgcaga caaaaaaaca aaacaaaaca aaacataact taaagaaaca
     2341 taaaaaataa tatgcactat tttagaggta agccttttcc tttaggcaat aaacttgata
     2401 gacaagcggg tgaatttggc acaaattttg ccattgtttc gcaaaatgca accgctattg
     2461 agttatgtct ttttgatgaa aatcaccacg aaacccgtat tccgatgttt tcttccggag
     2521 atacttggca catttttgta gcaggtgtac aagcagggac aaaatatggc tacagggtga
     2581 aaggcataca agatgaacaa gccggtttgt tatttaatcc gcaaaaatta ttgactgatc
     2641 cttacgctaa agcgattgaa gggaagccgg atttaagtag tgcagaggca gccgcttggt
     2701 ataaatggaa cgatgaacgg gataatgcct ctcttgcacc taagtctatc gtgattgatg
     2761 agcattttga ttgggaaaat gatagccagt tattcactcc ttgggcagag actattattt
     2821 atgaaatgca ggtgaaaggt tttagtcagc ggaactttca gattccaagc gacattcgag
     2881 gcacttttgc cggtttggct caccctgttt cgatagacta tttaaaaacc ttaggaatca
     2941 ccgcagtaga acttctaccc gttacttacc atattgatga aacccatttg cagaaacaag
     3001 ggcttgtgaa ttattggggc tataatgtat taggtaattt tgctattgat ccgaatttag
     3061 cagcagataa gcaaaatcct cttaaagagt ttaaacagct cgtaaaaaca ttacatcaag
     3121 cacatatcga agtgattatg gatattgtgt ttaaccacac agctgagagt gataaaaacg
     3181 ggccgatgct aagttttaga ggcattgaca atacagctta ttggctagat aaacagggcg
     3241 aatacgaaaa ttggactggc tgtggtaata ccttaaattt gagcaatcct tatatactct
     3301 gctgggcaat cgattgctta atctattggg tggaggagtg ccatattgat ggattccgct
     3361 ttgatcttgc ctcaattcta ggtagaacac cgagtttttc tgaaaaagca gattttttca
     3421 cagcacttaa aagccattct aaattaacac atattaagtt gattgctgag ccttgggata
     3481 ttggtgtaga aggttatcaa ttagggcaat tccctgagcc tttttatcag tggaatgatt
     3541 gttatcgtga tgatatacgc cgtttttggt tacaaaacca aggtgattgg gggcttttgg
     3601 cacggcgatt ggcaggaagt gatgatgtgt ttactaaata ttccgtttca aaaagtatca
     3661 attttattac ggcacacgat gggtttacgt tacaagatct caccagctat cagcaaaaac
     3721 ataattgggc aaatggtgaa caaaaccgag acgggcataa tagtaactac agcaataacc
     3781 acggtgttga agggcagaca gcagatgaat tggtaaaaaa acagagagaa atcaccgctt
     3841 gtagtttgct tgctacgctc tttttatcgg ctggtgttcc aatgctttta gccggtgatg
     3901 aaatcggaca tagtcaacaa ggtaacaata atagttattg ccaagataat gagatcactt
     3961 ggctagattg ggaaaatatg aatcacacac ggctggatta tgttcgtagt ttaatccggt
     4021 tgcgtaaaaa aatcgctttg ctttccaagc agaataattg gtggcaagag gattcggtac
     4081 aatggttgaa tccttctgct cagctaatga atattgacga ttggcataat caacataatg
     4141 ggcttgtcgt tttattacag gaagaatggc tgcttttatt caatgctaat cgacaacctc
     4201 aaccgttttt actgccacaa ggcaactgga aagttgtgtt ggagcaggct caaggtattt
     4261 tgatgacaga cacgcaagcg gttacgctca ataatatggg tgtatgcgta cttcaaaaaa
     4321 gtgatgtaat gttcgctaat aaattggaga cacaatatgc ttaaccaaga atcaacggct
     4381 cttgttgcaa gacaagattt aaccaacgat accttagtat taattcttgc aggaggacga
     4441 ggttctcgcc tatacgaatt aaccgataaa cgtgcaaaac cggcagtcta tttcggtggt
     4501 agccgccgta ttattgattt tgcgttatca aattgtatta actcgaattt attaaaaatc
     4561 ggggtgatta cccaatatgc agcacactct ttgttgcgcc atttacaacg tggttggtca
     4621 tttttacctt atgaacgtaa ccaatatatt gatatgttac cggcacgcca acagctagat
     4681 gaaaacactt ggtatcgtgg cacagcggat gcggtgtatc aaaatatggc gatgatgaaa
     4741 tcgcactata gaccaaaata tgtggtgatt ttagcagggg atcatatcta taaaatggac
     4801 tacactcaaa tgctgaaaga tcacgtcgat caaggtgcaa aatgtactgt aggttgtatc
     4861 gaagtgcctc gctcgcaagc aacagagttt ggggtaatgg cggtgaatga aaagctgaaa
     4921 gtgaaagagt tcgtcgaaaa accagctgat ccacctgcaa tgccgggaaa accggattcc
     4981 tcgctagcat caatggggat ttatgtattt aatgcagatt atctatatga aatgctggaa
     5041 aaagaagcct ctacaccggg aacctcacat gatttcggta aagatattat tccaaaagca
     5101 gtagagcaag gtgtgctata tgcccaccca tttgaacgtt cttgtaaagg gcgtaatgcc
     5161 agtggggcaa tttactggcg agatgtcggt acgattgaca gctactgggc tgcccatatg
     5221 gatttagttt ctgaagatcc ggaattagat ttatatgatg aatcttggcc gattcacggt
     5281 cgtccgcaac aaactgcacc ggcacgtttt ttctataatc gtgcgcgtgc cagaacccta
     5341 gataactcac tgattgcggg tggttgtgtg attaccgatg cggaaatcag taattcggtc
     5401 ttatttaacc gtgttcacgt tgaagcggag tcggtgattg aacacacagt gattttaccg
     5461 caggtgacta tcggtaaaaa ttgcgtaatt aagcgcgctg tgattgacag acattgtgta
     5521 attcctgatg gcttagaaat tggtgtaaat gccgaagaag atgcgaaacg ttttcgtgtc
     5581 agcaaaaatg gcattgtatt agtgacacag tcaatgttag ataaattagc gggaaaagaa
     5641 gctaattcgg atattaaata tacaatttag taagagatga tataatgctt cgagtttgtc
     5701 atttgaggtc gcaagctcga agtattgtta tgattaaagc gcttcaagcg gtcttatttt
     5761 tcaactattt tacacataga aaacggttaa aaatgaaagt tttacatatt tgctctgaaa
     5821 tgtacccttt aattaaaaca ggcggtttag ctgatgtaat gggggcattg ccatttgcgc
     5881 aacagcaagc tggtaatgat gtcagagtgt taattcctta ttacccgaaa gtcgcgcaaa
     5941 ctttagggga aacggtagaa gttgctacta tcggcacttt tgccggaaca gtctctgttc
     6001 gatttgctta ttttaatggc ttaggcgttt acgttatcga tgcgcctcat ttatttatgc
     6061 gtgatggtaa cccttaccac gacagcaatt atcaagacta tccggataac tataaacgct
     6121 ttgctttatt aggttacatt ggggcgcaat tagcccaagg tttggatcat tggtggggaa
     6181 ccgctcaagt tttacacgca cacgattggc aagcagggct tgcttgtgca tatcttaaaa
     6241 gttggggaag caatgtaaaa acgctcttca ctattcataa tattgcctat atcgggcgct
     6301 ttaatgcctt ccatttagca gagcttggct taccttggga atttttccac gccgaaggct
     6361 tagaatttta cggtgaaatt tcttacctca aagcaggttt gtattttgct gatcgcatta
     6421 ctaccgttag cccgacctat gctcgtgaaa ttactgagga aattgccggt gcaggcttgc
     6481 atggtttact ggcaacccgt caatatcaag gtagattgcg tggcatttta aacggggtcg
     6541 atgctgaggt gtggaatccg gaaacggata cacatattgt ggtgaattat cgtgcgaact
     6601 atatgcacgg taaagccaaa aataaagcgg aactccaacg aatttttggt ttgccagaag
     6661 acaaagaagc attattgatg gtaatggtga ctcgtttaac tgagcagaag ggggcagatt
     6721 tcttactcgc caatatagat accttaatgc aacataaggt acagattatt gtattgggaa
     6781 gtggatcgcc agatttagaa aatgcgctac gttatttcca acacaaatac ccgaaccaaa
     6841 ttggggtgaa aatcggttat gacgaggctt tatcgcacca aattattgca ggtggtgatc
     6901 tgattctagt accaagccgt tttgagccgt gcggattaac tcaactttat gggctgaaat
     6961 atggcacttt accgctggtt cgccgtaccg gtggcttagc tgatacggtg gtagatagcc
     7021 ataaagagag cattgaaaac cgcagtgcca ccggatttgt atttgattac ccagatgcac
     7081 ctgcgttttt agaggcagct atgcgggcaa tcagcttatg gcagaaacaa aaattgtggt
     7141 cgagcgttcg ccagaatgcc cttgctcaag attttggttg ggcaaaagtg gcagaacagt
     7201 accaaaattt ataccacgaa attatttaat ttgtattcac aacatcattt caagcggtcg
     7261 aatttgcgga tttttttgta aaaacgaccg cttgcaatat taaaaattag gagaatttac
     7321 tcaatgagca ggcagtcatt aagcccagaa atgatcaaaa atgttaaaaa tgcgattttg
     7381 cacaaaatgg tgttcgcttt aggggtcgaa ccccgtgatg ccagtaagcg taactggctc
     7441 aatgcaacct tacgtgtagt gcgtgattta tctaccgaag attggttaca aacccgccgt
     7501 agccaggttg caaatggtag ccgccgtgtt tattaccttt caatggaatt tttaatgggg
     7561 cgtacgttca gtaacgcaat gatttctgaa ggtgtgtatg agcttattgg cgctgcgtta
     7621 aaagagttag ggcaagatct ggaagagatt atcaatgaag agggcgtccg ggcttaggta
     7681 acggtggctt agggcgttta gccgcctgct atatggacag ccttgccaca atgaaaattc
     7741 cggcaatcgg ctacggtatt caatatgaat acggtatgtt ccgccaagaa atccgcaacg
     7801 gggagcaaat tgagcaacct gatgaatggt tagagaatga atttgtgtgg ccatatctac
     7861 gttcaagtaa gcgtttcccg gtacgctttg gcggtaaaac ttggcgtgag ggcaaaaaag
     7921 tagtatggca gccggaagaa gaaattaccg ctcaagctca cgaccaactg atcccgggct
     7981 ttgagaccac ctcaactaac agcttacgtt tatggtctgc acacgcatcg ggtagagggt
     8041 ttggcttatc tgattttaac cgtggcgatt attttgcagc aatggttaaa caaaacagct
     8101 cggaaaacgt ctctcgtgtg ctttatccgg acgattctac ctataacggt cgtgaattac
     8161 gcttacgcca agagtatttc ctttgctcgg catctgtaca agatattatc cgccgccacg
     8221 aagcggagtt cggctcttgt gtgaatttag ccgacaaagt ggcaactcac ttaaatgaca
     8281 cacacccaac ccttgcggtg cctgagttaa tgcgtatttt aattgacgaa aaaggctatg
     8341 gctgggaaca agcgtgggcg atgactcgta aaattttctt ctacaccaat cacactttga
     8401 tgagtgaagc gttagaaact tggccggtag aaatgttagc ccgtgtgcta ccacgccatt
     8461 tagagattat tttcgacatt aacgaatatt tcttagagga agtgcgtagc caattcccgg
     8521 aagatcacga tttgatttct cgtgtttcct taattgatga acaaggcgat cgccgtgtgc
     8581 gtatggcgtg gcttgcggta gtcgcctcag cgaaagtaaa tggtgtggcg aaaattcact
     8641 cggatttaat ggttgaatct atttttgccg attttgcccg tatttaccca aatcgtttta
     8701 ctaatgtgac taatggggtg acacctcgcc gctggatcaa aattgccaac ccagggcttg
     8761 ctaatatttt agataaatgt atcggcaata agtggctgac tgatttaagt gaattggaaa
     8821 aattcaacgt gtttgttgat gatgctgatg tgcaagcaca ggtggcagcg gtgaaagtag
     8881 aaaacaaacg tcgcttagcc aaatatgtcg aagaaactca gggaattaaa cttaatcctg
     8941 aagcgatttt tgatgtacaa gttaagcgta ttcacaagta taaacgccag caaatgaacg
     9001 tgttgcatat catcacgctt tataaccgca tcttgaaaaa cccgactgct gattggacac
     9061 ctcgtgtgtt tatcttcgca gggaaagcgg caagtgctta ttatgcggcg aaaaaagtga
     9121 ttcgtttgat taatgatgtg gcaaatgtgg tgaataatga ccctcgcatt aatgatttaa
     9181 ttaaggtggt gtttattccg aactacggtg tttcattagc acaaatgatt attccggcag
     9241 ctgatgtttc agagcaaatt tccttagcgg gtacagaggc ctccggaacc tctaatatga
     9301 aatttgccct caatggtgct ttaaccattg gtacattaga cggtgcgaat gtcgaaattt
     9361 tagatcacgt tggtaaagag aacatcttta ttttcggtaa tgaagtagag caggtggaag
     9421 agcttcgcca caacggttac tcaccgtatc actactacga aaaagacagc gaattgaatg
     9481 aggcaatttc acaaattcta aacggtaaat tctcaccgga agatccgtat cgctatcaag
     9541 atttgatctt aaattcaggt gactactacc aagcctgtgc cgattatcgt agctatgtgg
     9601 aggctcaaga aaaagtggct caagcctatc gtaacaagaa agcttggaca cgttcagcca
     9661 tcatcaatat tgccaatatg ggctatttct cctcagaccg ctcggtgatg gattatgcga
     9721 aaaatatttg gcatatcgaa ccgatgagcg aagaacaatt aaaaggcgat tctacaatgg
     9781 gcagtattat tgaaagttca aataaactgt tgaatttaaa ataatgattt agagaaaacg
     9841 agtaatctta ccgtttttgt tttagtcagg gattttgcaa atttctacta aaaattaacc
     9901 gcttgtcaat ttgccttctc caccgccgtg agcattcccc tcagtaaatt cagctcatta
     9961 gtttccagtt cggcacgttg gtataaacgg cggagtttta gcatcactga atcattgcgg
    10021 ataaagccta attcggtata taagcgttcg gtgtgagcaa agaaatgctc taaggcttga
    10081 gcggttgggt aatccttatc tgctgcaagc ggttgttttt cgccagaatt ttgcaaataa
    10141 gccattcgga tttcataact tgcgagctgt actgccattg ccaaattcag agaaccataa
    10201 tcaggattgg ttgggaaatt caaatggtac tggcatttca ataattcttc gttggttagc
    10261 cccactcgct cacgtccaaa tacaatcgcc actttgccaa gattggttcg atttaccact
    10321 aattcaccac agtctcttgg ttcaatcagg gtattttgca aatggcgaag tctggcactg
    10381 gtgccgatca ccagttggca atcggcaatg gcttcatcga aagtggcgta gcattgagcg
    10441 ttatccaaca catcttttgc cccagcagca agggcttcgg cttgttcatc aagcggttgt
    10501 tttggggcga ctaatcgcaa atgggttaag cccatcgttt tcattgctcg ggctgcggaa
    10561 ccgatatttg ccgggagtga agtttcaaca agaatgatct gaatttggtt gagaatctgc
    10621 attggttttg tttctttttt gttaaattta gcctgttaat agtgtagcaa aaatttagcc
    10681 tgcttaaaat ttgaaaaaat gggaaataat cggtttaatt gggcataatt ttttacctat
    10741 ttgttttatt tagtggtaca tttagagcgt ttttaatcac aacaaccata caggagtctt
    10801 tatgcttatt ggtgtaccaa gagagctgtt agacggtgaa actcgagtgg cggcaacgcc
    10861 aaaaaccgtt gagcagatca aaaaacttgg ctttgacgta cttgtggaac aagacgcagg
    10921 ttttaaagcc agttttgaag ataatacatt tttaaatgca ggtgcaaata tcggcacaca
    10981 acaagaagtt tggaatgcag acattatttt taaagtgaat gcgccgacag aagctgaaat
    11041 tgagttaatc aaagaagggg taaccttagt cagctttatt tggccggcac aaaatccaca
    11101 attgatggaa aaattgcagt ctaagaaaat taatgtgttg gcaatggacg ctgtgcctcg
    11161 tatttcccgt gcccaagctc tcgatgcttt aagttcgatg gcaaatattt cgggttaccg
    11221 tgcggtgatc gaagcggcaa acgcttttgg tagcttcttt actgggcaaa ttaccgcagc
    11281 aggtaaagta ccaccggcaa aagtgttggt aattggagcc ggtgttgctg gcttggctgc
    11341 aatcggggcg gcaaatagcc tgggggcgat tgtgcgtgcc tttgactctc gtcctgaagt
    11401 aaaagagcaa gttaaatcaa tgggggctga tttcctcgaa attgattttg aagaagaagg
    11461 tggctcaggt gacggctatg cgaaagtgat gtcggaagag tttaaccgtc gtgcaatggc
    11521 gttatatgcc gagcaagcca aagaggtgga tattattatt accaccgctg ccattccggg
    11581 caaacctgct cctcgtttaa tcactaaaga gatggttgat tcaatgaaac cgggttcagt
    11641 gattgttgat ttagccgctg caaccggtgg taactgtgaa tacaccaaag ccggcgagat
    11701 tgtggtgacc gataatcaag tgaaagtgat tggctatacc gattttcctg cacgtttacc
    11761 aacgcaatct tcgcaacttt atggtactaa cttggtgaat ttactcaaac tgttagcacc
    11821 aaataaagat ggtcaaattg agattaattt tgatgatgta gtgttgcgtg gcgtaacggt
    11881 ggtgcgtgac ggcgaattaa cttggccggc tccgccgatt caagtatctg cccaactgca
    11941 aaaacaagca gccgcaccac aagcggtcaa aaaagaggaa aaacctgcag accctcgagt
    12001 gaaatatggc gtgatggcag cagcaggtgt gctgttccta tggcttgcat ctgtggctcc
    12061 ggcagcgttt ttatcgcatt ttaccgtatt cgttctcgct tgtgtggtag ggtattatgt
    12121 ggtttggaac gtcagccacg cactacatac cccattaatg gcggtaacaa atgcgatttc
    12181 agggattatc atcgtaggtg cggtattaca aattgcccaa ccgaccggta atttctttat
    12241 tgatattttg gcatttattg cgattttggt tgccagcatc aatatttttg gtggctttaa
    12301 agtaacgcaa cgtatgcttg caatgtttag aaaaggttaa ggagaaacga aactatgtct
    12361 tttggatttg tgactgctgc gtacattatt gcagctattc tctttattat gagcttagcc
    12421 ggtctttcta aacacgaaac cgccaaagca ggttgttggt atgggattgt gggtatggcg
    12481 attgctttag tggcaaccat tttcggcccg caatcacatg gcacgatttg gatcttaatc
    12541 gcaatggcaa tcggtggatt catcggggtg aaaaaagccc taaaagtaga aatgaccgaa
    12601 atgcctgaat tagtggcaat tctacacagc tttgtcggtt tagcagcggt attggttggt
    12661 tttaacagct acggacttca taccaatgca ttaatgcctg caaatttaga tgaaactgca
    12721 caagcggcat ttttagcgga acaagccact cttgccaata tccacaatgt ggaagtgttc
    12781 ttaggtattt tcattggtgc ggtaaccttc tccggttcat tggttgcctt tggtaaacta
    12841 agcggtaaat tatttggtcg taaagtctct tctgcagcac tgaatattcc acataaacac
    12901 aaatggaatt tggcagccat tatcgtttct gtttttttga tgatcgtgtt tttaaataat
    12961 ccgcacaata tcttccctgt gctgattatg acaattattg cgttagtctt cggctggcac
    13021 ttagtttctt ctattggtgg ggcggatatg ccggtggtag tttcaatgct gaattcttat
    13081 tcaggttggg cagccgcagc ggcaggcttt atgctaagca acgatttatt aatcgtaacc
    13141 ggtgcattag tgggatcatc aggtgctatc ctttcttata ttatgtgtaa agcaatgaac
    13201 cgctcattca ttagcgtgat tgccggtggt tttggcaatg aagttcagtc tggcaaaggt
    13261 gatgaagaat acggtgaaca ccgtgaaacg aaagccgaag aagtggcaga aatgctgaaa
    13321 aatgcaagct ctgtgatcat cacgccgggt tatggtatgg cagtggctca agcacaatat
    13381 ccggtagcgg aaatcaccgc aaaattgcgt gaaaaaggcg tgaatgtgcg ttttggtatt
    13441 caccctgtgg cagggcgttt accggggcat atgaacgtat tattggcgga agcgaaagta
    13501 ccttatgatg tagtgctgga gatggacgaa atcaatgacg attttgaaga aaccgatgtg
    13561 gtattggtaa tcggtgcaaa cgataccgta aaccctgctg cattagacga cccatcaagc
    13621 cctattgctg gaatgccggt gcttgaagta tggaaagcac aaaatgttat cgtgttcaaa
    13681 cgttcaatgg cagtaggcta cgccggtgtg caaaacccac tgttctttaa agaaaatacc
    13741 caaatgctat ttggtgatgc aaaagaacgt gttgatgata ttttacgggc gttaaattaa
    13801 atttaaataa aaaagcgaac ataagttcgc ttttttgtgc ttaggctaac ataaattgtt
    13861 aattttttac tctaatacca tcaatccggg aagagtcgca aaactctgcg attttacggt
    13921 atcatggaaa tcatctaagt aggctagggt agcatcggct tctcgaatag cgccataaag
    13981 ggtgctatat agtccttgct gagttatttt tcttgccacc acataccccc tatctaaata
    14041 tggtttaacc gcccaataag gtagtgcagc aatgcctcgc cgacttgcta ctagctgaat
    14101 aatagcaata gttaactcac tattacgccg agtaactttg atgccagcag ggcctaatat
    14161 ttttcgcagt aaatctagca tatcatcagg tactggatag gtaattaatg tctcatcttt
    14221 aaaatcttca gcttcccata catcttttgt ggcaagaggg tgatctttag agcaaatagc
    14281 taccatttca tatgaaaaga gaggtttgta tataatacca gcactatctt cctcttcagc
    14341 gacaattgcc agatctgcac gatggcttaa tagcaaacca accgtatcag tatggaaacc
    14401 cgatacgata tctaattcaa ctagaggcca atgttgacgg aaagtatcca ttgccggcat
    14461 tagccaatca aaacaggtat ggcattctac tgcaacacgt acctctccga cttctccttg
    14521 tttcactcgg gctaaatcta attcagcctc aatgactttg ggcaaaatat cgtaggccaa
    14581 cttaattaaa cgttcgccag ctggtgtaaa gcgaataggg gagctttttc gctcaaatag
    14641 cgtaagatca tactgttctt ccagtaattt tatttggtgt gaaagtgcgg attgtgttaa
    14701 atggacacgc tttgctgcaa gagatacgct accgctctct tttaatgcga ttaatgtccg
    14761 caaatggcgg atttcaagaa aaatcggttt cataatttat tcatctaaaa gtgaaaaaag
    14821 atgaaaataa ttataacaaa caagcggtca aattctgcaa aaaatttgca aaatccaacc
    14881 gcttgtatag agcagtggcg aattattggg ttaattgaac taaaatgcgg cgaacaggct
    14941 ctgctgcacc ccataataat tggtcaccta cggtaaatgc agctaagtat tctccaccca
    15001 tcgctaattt acgcaagcga ccaaccggca cgctcaatgt accggtcact ttcgccgggg
    15061 tcaattcacg caatgtggtt tctttgtcgt ttggaattac tttcacccat tcattgtggg
    15121 cagcaataat ttgctcgatt tccgctaacg gaatatcttt tttcagctta attgtgaatg
    15181 cttggctgtg gcagcgtaat gctccaatac gcacgcataa accatcaact ggaattgggt
    15241 tatcgcttaa acctaaaatt ttattagttt ctgcataacc tttccactct tctttagttt
    15301 gcccggtttc cggtaataat ttatcaatcc aaggaattaa gctaccgccc aatgccgcac
    15361 cgaagttctc ggttgggaag tcttccgcac gcattttagc cgtaacttta cgttcaatat
    15421 ctaaaataga agaggcagga tttgctaatt cagcttttac gctatcttct aattcaccca
    15481 tttgagaaag caattcacgc atatttttcg caccggcacc ggaagcggct tggtaagttg
    15541 ctacagaaac ccactcgacc aaatctttct caaataaacc accgatagcc attagcatta
    15601 ggcttacggt acagtttccg cctacaaatg ttttaatgcc gttttttaag ccttctgaaa
    15661 tcacatgttg gttcaccggg tctaatacga taatagcatc gtctttcata cgtaatgcag
    15721 aagccgcgtc aatccaatag ccgttccaac cggtttcttt taattttgga tagacttcat
    15781 ttgtataatc accaccttgg caagttacga taatgtctaa tttttttaat tcttcaatgt
    15841 caaaggcgtt ttttaattcg ccggcatctt ttccgccaaa aacaggggct ttttgacccg
    15901 cttgtgaagt agtgaaaaaa acagggttga tattggcgaa gttattttct tcaaccatac
    15961 gatccattaa gaccgaaccg accataccac gccaaccgat aaaacctaca ttttgcattg
    16021 tgtttgctcc taaaaatgat gttataaaga ttgtgaat
//
LOCUS       DANLXQ010000048        15245 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.048, whole genome shotgun sequence.
ACCESSION   DANLXQ010000048 DANLXQ010000000
VERSION     DANLXQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15245
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.048"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            52..900
                     /locus_tag="PXD94_001122"
     CDS             52..900
                     /locus_tag="PXD94_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL6285997.1"
                     /translation="MEKKALFRGFLLSTVALAVAACGNLSKVSDEGTTENPVFPKISE
                     SEFNHDGSQFGSWPNWENVRQIEKGMNKDQLYNLIGRPHFEEGLYGVREWDYAFNYRE
                     NGVHKICQFKILFDKNMNAQSFFWYPNGCNGNSSFALTGDFLFDFDKATLTTKGKEVV
                     DNVAQQLKASKAQQVKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVG
                     YGKANQVKACDGETGQALKDCLRPNRRVEISANCGVMKQNEGGNVAGPNGPAPLYQTP
                     AYDGSK"
     gene            988..1806
                     /locus_tag="PXD94_001123"
     CDS             988..1806
                     /locus_tag="PXD94_001123"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL6285998.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            1809..3500
                     /gene="narQ"
                     /locus_tag="PXD94_001124"
     CDS             1809..3500
                     /gene="narQ"
                     /locus_tag="PXD94_001124"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL6285999.1"
                     /translation="MIKAKYSIARRIGLYFLIMIGFASIISGISLGIMWSNKSDASLI
                     NVSGSLRMQSYRLLSEMDSKKALLPERLLEYHRTLHATELSSLKQSVLLPDSVIESYQ
                     QLLKDWYEMQSYVAQGDKVNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAM
                     LLIIALAYIGVWYTRKKIIGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLSSTFT
                     EMSNELLKLYTSLEDKVQDKTRRLLAVNRSLLVLYQCSQLLTAKPINKEVLNQVLKTV
                     FDNEHLLGIDLQVYGAEYWNVSIDNKPSLDWVSAEIAIENEKLGILSWKPSLVCPGER
                     LIQNVSEMIGRSLYVVQMQKQQQQLVLMEERAIIARELHDSLAQSLTFFKIQVSLLKL
                     AKTDEKRNEILTVFEKALNDAYSQLRELLTTFRLTIQEANLQQALEKILESLRPKTSA
                     QIRLSCKLPSHMFNAQQQVHVLQIVREAVINAIKHSQATEINVIAETNDDGEHCLTIQ
                     DNGVGLAAHTQPEGHYGLTIMQERAAELKAEFSIENVESGGVKVQVLLPSMISKR"
     gene            3551..4069
                     /locus_tag="PXD94_001125"
     CDS             3551..4069
                     /locus_tag="PXD94_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL6286000.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEKGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            4112..4711
                     /gene="rdgB"
                     /locus_tag="PXD94_001126"
     CDS             4112..4711
                     /gene="rdgB"
                     /locus_tag="PXD94_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL6286001.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTGLPAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            4708..5028
                     /locus_tag="PXD94_001127"
     CDS             4708..5028
                     /locus_tag="PXD94_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL6286002.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVATGTA
                     VVIR"
     gene            5032..5484
                     /locus_tag="PXD94_001128"
     CDS             5032..5484
                     /locus_tag="PXD94_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL6286003.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(5525..6106)
                     /gene="lpcA"
                     /locus_tag="PXD94_001129"
     CDS             complement(5525..6106)
                     /gene="lpcA"
                     /locus_tag="PXD94_001129"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL6286004.1"
                     /translation="MYLEQIKAELQEAADVLDKFMSDEKNIKLIQDAAMLIANSFKQG
                     GKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHLSCVSNDFGYEYVFS
                     RYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVIAMTGKDGGQMAGLADVEI
                     RVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
     gene            6415..7374
                     /locus_tag="PXD94_001130"
     CDS             6415..7374
                     /locus_tag="PXD94_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL6286005.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            7367..9118
                     /locus_tag="PXD94_001131"
     CDS             7367..9118
                     /locus_tag="PXD94_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6286006.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVILSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            9121..10086
                     /locus_tag="PXD94_001132"
     CDS             9121..10086
                     /locus_tag="PXD94_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6286007.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFPATITLCLSALIVSLIIGLPLGFFAATQ
                     QKNMVGRLLNTLGSLSLAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFL
                     LLDIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIA
                     KTRGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINAL
                     AIQDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
     gene            10073..10966
                     /locus_tag="PXD94_001133"
     CDS             10073..10966
                     /locus_tag="PXD94_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL6286008.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASCWYLIREVIIPNLTGVAIK
                     ALSHIFVIAILDISALSFIMLGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFS
                     IFTVTILGTRLSVIFEKYRKE"
     gene            11037..12089
                     /locus_tag="PXD94_001134"
     CDS             11037..12089
                     /locus_tag="PXD94_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6286009.1"
                     /translation="MALLDIRHLSIEIDMPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNLGTSILLVSNDMRS
                     IQNWVDSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLAFKSSLNTLR
                     GSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFREQNLLKNKT
                     ENTPFVVAEIAEKEAF"
     gene            complement(12098..12328)
                     /locus_tag="PXD94_001135"
     CDS             complement(12098..12328)
                     /locus_tag="PXD94_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL6286010.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            12458..13540
                     /gene="leuB"
                     /locus_tag="PXD94_001136"
     CDS             12458..13540
                     /gene="leuB"
                     /locus_tag="PXD94_001136"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL6286011.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLLPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(13627..14022)
                     /gene="msrB"
                     /locus_tag="PXD94_001137"
     CDS             complement(13627..14022)
                     /gene="msrB"
                     /locus_tag="PXD94_001137"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL6286012.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
     gene            14247..>15245
                     /gene="gap"
                     /locus_tag="PXD94_001138"
     CDS             14247..>15245
                     /gene="gap"
                     /locus_tag="PXD94_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL6286013.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVAHVYNYK"
BASE COUNT         4794 a         2610 c         3257 g         4584 t
ORIGIN      
        1 ttgtgaagct atttttttat acagattact ataaggaaac ttaatatgaa aatggaaaaa
       61 aaagcccttt tccgtggatt tttattatcg acagtggcat tagcagtggc tgcttgtggt
      121 aacttaagta aagtgagtga tgaaggaaca actgaaaacc cggtattccc gaaaatctca
      181 gaatctgaat ttaaccacga tggttcacaa tttggttcat ggccaaactg ggaaaacgtt
      241 cgtcaaattg aaaaagggat gaacaaagat caattatata atctaattgg tcgtcctcat
      301 tttgaagaag gcttatatgg tgttcgtgaa tgggactatg cattcaatta ccgtgaaaat
      361 ggcgtacata aaatttgcca atttaaaatt ttattcgata aaaatatgaa tgcacaaagt
      421 ttcttctggt atccaaatgg atgtaatgga aactcatcat ttgctttaac tggcgatttc
      481 ttattcgatt tcgataaagc gactttaact acaaaaggta aagaagttgt tgataatgtt
      541 gctcaacaat taaaagcatc taaagcacaa caagttaaag tagcaggcta tacagaccgt
      601 ttaggctctg ctgcttataa cttagactta tcacagcgtc gttctaatac agttaaagct
      661 cgtttagtac aacaaggcgt tacagcacaa attgaagcgg taggttatgg taaagcgaat
      721 caagtaaaag cttgtgatgg tgaaactggt caagcattaa aagattgttt acgccctaac
      781 cgtcgtgttg aaatttctgc aaattgtggt gtaatgaaac aaaatgaagg tggcaatgtt
      841 gcaggtccta atggtccggc tccactttat caaaccccag catatgatgg cagtaaataa
      901 tttctagttt tgactaactt agagtagaat attaaggcaa ccaaatggtt gccttttctt
      961 ttatctattt atttataggg gaatactatg aactaccaag ctattgcatt tgatttagac
     1021 ggaacactac tttcatcgaa cgcaacaatt ttagaatcca gtaaagaggc tattaaaaag
     1081 gtaagagaaa agggaattaa agtctacttt gttaccggta ggcaccatac tgcggttcgc
     1141 ccttattatg tagaactagg gctggatacg ccagttgtgt gttgcaatgg cacttatgtg
     1201 tatgatttta aaaatgaaaa agtattaagc ggtaatccgt tatcagcaga aattgcttca
     1261 aaattagtta atgatgcaca agctcaaggg attcatactg cggtttattt tgaagatgca
     1321 atgacttatg agtctttgaa tattcacttc actaaattcc aaaaatgggt tgagtcttgc
     1381 gcaccagaag ttcgtccgaa tgtttatcag gtagaaaatt tccaacaaga aattgataaa
     1441 ggtgtaaccg tatggaaagt gcttattagc gatccagatc ttgccaaaat gcaaaaattt
     1501 gttgaaaaat taccgcctga tcaagtaagc ccggaatggt cttgggttga tagggttgat
     1561 attaccagtg ttggtaacag caaaggggca agattagcgg aagtattaaa aacagaaggt
     1621 attgatttac aaaaggtgat tgcctttggc gataatttca atgatgtttc tatgctagaa
     1681 accgttggat taggcattgc aatgggaaat tcagaaccag atattcagca aaaagcgaag
     1741 aaaactatag gttcaaataa tgaagatagt attgctaatg aattgaaagc tcaatttaat
     1801 ctttaattat gataaaagca aaatactcta ttgctaggcg gataggactc tattttttaa
     1861 ttatgatcgg atttgcttcg attatcagtg gaatctcact tggtattatg tggagcaata
     1921 aatctgatgc gagcctgatt aatgtttcag gctcacttcg tatgcaaagt tatcgattgt
     1981 tatcagaaat ggattcaaaa aaagcgctgc tacctgagcg attgttggaa tatcatcgaa
     2041 cgctacatgc tacagagctt agttcattaa agcaaagtgt tttattgcca gatagcgtga
     2101 tagaaagcta tcaacaatta cttaaagatt ggtatgaaat gcaatcttat gtcgctcagg
     2161 gagataaagt taattatgct tcgcacattg aagattatgt gcagagagtt gatgattttg
     2221 ttttccgctt acaagagttc gctgagttaa aattaaagat tgcaacaggc gtgattgcga
     2281 ttgcaatgtt actgattatt gcccttgctt atattggcgt ttggtatact cgaaaaaaga
     2341 ttattggacc tttaaggcaa ttggtaaaag cgagttggca aattcaaaat aaagattttg
     2401 accacgtaaa attagcggtt aatgaaccca atgaattagg gctactttcc tctactttta
     2461 ctgagatgtc taatgaatta ttaaaattat atacttcatt agaagataaa gttcaggata
     2521 aaacacgtcg tttattggcg gtaaatcgtt ctttactggt attatatcaa tgctctcaat
     2581 tattaactgc taagccgatt aataaagaag tgttaaatca agtacttaaa acggtatttg
     2641 ataatgaaca tttgctaggg attgatttac aagtatatgg ggctgaatat tggaatgtat
     2701 caatagacaa taaaccaagt ttagattggg tttctgctga aattgcaata gaaaatgaaa
     2761 aattgggtat cttaagctgg aaaccctcac tggtttgtcc tggtgaacga ttgatccaaa
     2821 atgtgtcgga aatgattggt cgtagccttt atgtagtgca gatgcaaaag caacagcaac
     2881 aattggtact catggaggag cgtgcaatta ttgctcgtga gttacacgat tccttagccc
     2941 aatctcttac gtttttcaaa attcaggtta gcctgcttaa gcttgctaaa acagatgaaa
     3001 agcgtaatga aattttaact gtatttgaaa aagcgttaaa tgatgcttac agccaattaa
     3061 gggagctgtt gacgacgttt aggctaacga ttcaagaagc gaatttacaa caagcgttag
     3121 aaaaaatttt agaaagctta agaccgaaaa cctcagcaca gattcgctta agttgcaaat
     3181 taccttcgca tatgtttaat gctcagcagc aagttcacgt attgcaaatt gtacgagaag
     3241 ctgtgattaa tgctattaag cattcgcaag ccactgaaat taatgtgatt gctgaaacaa
     3301 atgatgatgg tgaacattgt ttgactattc aagataatgg cgtagggctt gctgcccata
     3361 cgcaaccaga agggcattat ggactgacga ttatgcaaga acgggcggct gaactcaaag
     3421 ccgaattttc gattgaaaat gtggaaagtg gcggagtaaa agtacaggtt ttattgccga
     3481 gtatgattag caagcggtaa taaaatatta attttttgcg tattattggc aacgaataaa
     3541 aggataacaa atgcaattcc gaaaagcagt agaagcggat tttgagacaa tattggcaat
     3601 ttataatcaa gcaattccca ctcaccaaat taccgccgat ttagaactgg caactacgca
     3661 aaatcggcgg aagtggttcg attttcacct tgaaacagcc caatatccta tttgggtggt
     3721 cgaagatgaa aaaggcattg ctggctggtt tagtttttca ccattttatg aaagaccggc
     3781 atttttacat acttcggaaa tcagtatcta tttagaccgc tattccaaag gaaaaggcta
     3841 tggctcaaaa attattgagt ttatgcaagc tgaaatgctt aatcacaata ttcatacgtt
     3901 gatggcgtat gtgtttgaat taaatacagt gagccaaaat ctaatgagaa aacacggctt
     3961 tgaacaatgg ggaagatttc cgaatattgc taatatggga aaagatgaga aaggtgagga
     4021 taaatggcga acgttgctga tgatgtctta ccaaaaaggt atcgaataac aagcggtgat
     4081 tttttagaat atttttgcaa ataaggacaa tatggaaaag acaaaaattg tacttgcaac
     4141 aagtaatgca gataaagtta aagaaatggc agatgtactc tcacaatttg gttttgaggt
     4201 ggtggctcaa agtgaatttg ggattgaatc accggaagaa accggtttga cttttgttga
     4261 aaatgcgctg attaaagcac gatttgctgc taaaatgaca ggtttaccgg cgattgccga
     4321 tgattcaggt ttatcggtta tggcacttgg tggtgaaccg gggctatatt ctgcacgtta
     4381 tgccggagag caggcaaccg atgccgataa tcgccaaaaa ttactagcca aaatgcaagg
     4441 gcaagagaat cgtctggcga aatttgtcag ttgtatcgtg tttttaaaac acgaaactga
     4501 cccaacacct tatattgcgt cgggtgaatg tttcggtgaa attttgacgg aagaaagagg
     4561 tgaaaatggt ttcggctacg attccctgtt tttctaccca ccgaaagctt gcacttttgc
     4621 cgaattagaa accaaagaga aaaaagcgat ttcacaccgt gcaatcgcac tagagcaatt
     4681 aaaacagcaa ttagcaggag agaataaatg attattacaa ctacacctac gatagagggt
     4741 aaacaaattg tagaatacaa agggttagta tttggcgaag tagtttctgg ggctaatttt
     4801 atccgtgatt tcttagccgg tatcaccgat attattggcg gacgttcagg tgcttatgaa
     4861 agcaaattaa acggtgcaag aaaagaggca ttagctgaat tagctaagga ggcacaaaaa
     4921 gtgggggcaa atgcggtggt tggcgtttct atggagtatc aatcaatggg cggcgataaa
     4981 ggaatgttca tcgtggtagc gactggtact gcggttgtaa tacgttaatt tatgcagttt
     5041 gaacaacact ttgaagcaaa tttctggcag aacaaatcgc tacttgaaat gaatgatgct
     5101 gaatgggaag cattatgtga cggttgtggt aagtgctgct accggaaata tattcaggga
     5161 agagggcggc gtgaacggct ttactatacc tcggttgcct gtaatttatt agatgtgaat
     5221 acaggccgtt gtatgaatta ctcgaaccgt tttaaaattg agtcggattg tacaaaatta
     5281 actaaaaaga acttacctga ttttggctgg ttgccaaaaa cctgtgctta tcgtttactt
     5341 tatgaaggaa agcctctttt tgattggcac cctttaatct ctaaagatcc tgattctgta
     5401 aaaaatgccg gaatgctgat tgctaacggt attcacgaaa aagatgtgat tgattggttt
     5461 gagtttgtta ttgatgaagt atgagaaaaa ggcgtttggt gcaaacgcct ttttgttata
     5521 gagattaggc tttttccatc tcaaattcaa ttaacatcat caaaatatga atcactttaa
     5581 tgtgaatttc ttggatgcgg tctgcgtaac ggaaatgagg tactcggatt tccacatcgg
     5641 ctaatcctgc catttgcccg ccatctttgc cggtcattgc aattactttc atgccttttt
     5701 ctttggcaac tttgatcgca tttaacacat ttttagagtt accagaggtt gatagaccaa
     5761 agagtacatc gcctttttgc ccaacggcct ctagataacg agagaagaca tattcataac
     5821 cgaagtcatt acttacacag cttaaatggc tgacatcgga aatagcgatt gccgggtatc
     5881 cgggacggtt ttcacggtaa cgacctgtta attcttcggc aaagtgcatc gcatcacagt
     5941 gcgaaccgcc attaccgcaa gaaatcacct taccaccttg cttgaaacta tttgcaatca
     6001 gcatggcggc atcttgaatt aatttaatat ttttttcatc agacataaat ttgtctaata
     6061 catctgcagc ttcttgaagc tcagctttaa tttgttctaa atacatcatt tgctcctttg
     6121 tttgcaaaac gcttaccttt aaacatcggc tttaccaatg ggggtagcga gtaaaaaatt
     6181 cgttttttat gaccgcttga ggcataaaaa gtgcaataag tttagaagat ctatgctgta
     6241 ttgtaaacct aataacttaa ataatctgaa tagggttgta aacaaaagtt tccaaaagag
     6301 aaaaatagcg gtatgatcgc ctcacaatcc ctctcgcatt gttaactcta gtagctaata
     6361 tcaggcttta agcgtattaa acaatgcaca aatttgctat ttataggaga ctatatggtc
     6421 gtaaatccag aatcttttga acagataatt ggacaatctg taaagataaa agaggttgtt
     6481 gaacaagcaa aaaaatttgc caaccttgat gcaccattat tgattcaagg tgagacggga
     6541 acaggaaaag atttatttgc caaatcttgt caccattttg gatctcgtcg aatgcaaaaa
     6601 tttattgccg tgaactgtgc cggtttaccg gctgaagaag cagaatcgga aatgttcgga
     6661 catcgtggca atggcgttga gtccgtcggt ttctttgaat atgctcacgg tggtacagta
     6721 ttattagact ctatttctga gttatcgctg gaaatgcaag ccaaattatt gcgcttttta
     6781 aatgatggct cttttcgtcg agtgggcgaa gatcaagaga ttcaagtcaa tgttagggtg
     6841 atctgtactt cattacagcc tttgtcatta ttagttgaaa aaggcaaagt aagagaagat
     6901 ctgtttcatc gtttaaatgt tctcacttta aatttaccac ctttgcgaga tcgaaaagag
     6961 gatattccat tattagctga ttactttatc gcccaaatta gcaatcagtt gggggtgagc
     7021 aaattagagt atgataatgt ttttttgcaa gcattgcaga attatcactg gcgaggcaac
     7081 ttaagagagc tttataacgc actttatcga gcttgtacac tttcgcatag ctaccgttta
     7141 gcggtaaaag atcttcattt acccagagat atggaggcga tgattgaagt acgtgaggag
     7201 gcgacacttg aggaaatgat gagccagtat gaagcccaat tattgcacca attttatgca
     7261 gaatatccga gtacccgaaa attagcacaa cggctcggac tttcacatac tgcggtggca
     7321 aacaaattgc gtcaatatgg gttaacaaaa aatggaaaca gtaaagatga ataaactagc
     7381 ggtcattttt tcagtatgtt ttgcaaattt agggctaaat tcgaccgctt acgcagcgcc
     7441 aagtattcct caagagctac ttgaaaatag ccttgtgtat tgcactactt catcgggatt
     7501 ctcttttaac ccacaaaaat ccgatgtgag ttcgaatatg aatgtggtaa cagagcagat
     7561 ctacgataaa ttagttgagt ttaatccgaa aaaaaatcga ctagagcctg ctttagctga
     7621 aagttattca atcagcgacg atggtttagt gattacatta cgtttaagaa aaaatgtcgc
     7681 ttttcataca actccatggt ttactccgag tcgtaaatta aacgcagaag atgtggtgtt
     7741 ttcgctaaaa cgaatgattg gtaaagaaag tgatttacct gaagtgaaaa gtgagcctga
     7801 aagtgtatta caccaccagc gacaaagcta tatttaccaa aaaacagcgg ggcagattta
     7861 ttttccttat tttgaaagtg ttgatttagc gaataaaatt gccagcattc attctccgtc
     7921 aaatgacatt gtaaaaattg aattagtccg tcctgattca gcattgttgg cgcatttagc
     7981 cagccaatat gcagttattc tgtctaaaga atatgcttta cagctcaatg cagatgataa
     8041 cttagttcaa ctggatttat taccggtagg aacaggcgta tatcaacttg aaagttatga
     8101 aaataatgat tatgtgcgtt taaaaccaaa tatgaattac tggggcaaaa aagcatatat
     8161 tcaaaatatg gtcgtagatt tttccacaga agcgaccggc aggatggcaa aatttttaaa
     8221 taatgaatgt gatgtggttg cttttccgga acctagccaa ttatcagtgt taaataatcg
     8281 gcagcttatt agtagtaaag gtgctaattt ggtttattta gcattcaata tgcaacgggg
     8341 tattgggcag gatttggcat tacgccaacg tattgcacgc ggcataaatc gcaaacgtat
     8401 tgcagagaaa ttattttatg gtacaggtga agtagcggat aacctattac caacagcttt
     8461 gtggtcgaaa aaaaataatg gaagctaccc ttatcaacca caccaaaatt caggtgagca
     8521 agcggttgaa atgcatgaca gtattataaa tcggtcttta gatagcaaaa cagagaccga
     8581 agaagtaaac caaaaacagc gcttaaaact gtgggtaatt gatgaaaaac gagtttataa
     8641 tcaacaccca ttaaaaatgg cagaaatgat acgtgctgat ttagctacgc aaaatttaga
     8701 ggtcgaaatt cgcgcagtaa gtcgagctta tttggtgcaa cagcttgcta ataaaagtgc
     8761 ggattatgat ttgattttag gtggttggtt agcaaataat cttgatccta acagtttttt
     8821 ggaggcctta ctcagttgta aagctgcgga aacggtgact aatttatcaa attggtgcaa
     8881 tgaagatttt gatttttggt tacaaacagc ccgtctaagt gatgatccgg catcagctaa
     8941 tttattgtat gaatttgcac aaaaattatt agaagagcaa ttgcctattc tccctttaat
     9001 gaatgctaat cgtgtattag tcataaaaga tggagtgaaa caggcagata tttccccttt
     9061 cggccaggta aaattatcag agttacgttt gggcttgggg cataaaaaag gaaactaatt
     9121 atgctgattg tcttttttcg aaaaattttt ctaaccttga ttacgttgat ttttttgtca
     9181 cttatcagtt ttaatattct gttacgagat ccattaaacc acttagccga gttaggctgg
     9241 tttgaggctt attgttatta tgtaaaatct ctgctaaatg gtgattttgg tattagtttc
     9301 agtaatggag aaccgctcac agcgcaaatt ttacaggtgt tcccggctac aattacgctt
     9361 tgtttatcgg ctctcattgt ttctttaatt attggtttgc ctcttggttt ttttgcggct
     9421 actcagcaaa aaaatatggt tgggcgatta ttaaatacac taggttcatt gagtttagcc
     9481 gtgccggtat tttggctggc attagtgcta atgtattacg cttctctcaa tcagtgggaa
     9541 gtgtcagcaa ttggagaaat ccattcgctt tatccgaaac agatggtaac cggctttttg
     9601 ctattagata tttggttatc tgatgtacct tataaattaa aaatgatgca gagtgcttta
     9661 catcacttag ctttgccgac attagtcttg gctgtgccag ctacattaga agttatgcat
     9721 attactcaag ctagagcagg ttatgtaatg aaacaaaatt acattaaaat agcgaaaacg
     9781 cgtggctgga caccgtttag agtttggaga acgcatatta tttgtaatac tttacctgca
     9841 ttattaccta tgattgcccg tacctttatt ttaatttttg ccttcggtat gttaattgag
     9901 aacatattta gctggggagg aattggacgt tggctgataa atgcccttgc tattcaagac
     9961 tataatgcga tttcagcggg agtagttgca atcggggtgt ttgtgttatt agtagatatg
    10021 gttacaggct ttgtgcgggt gatgttagac ccatcagata aaaaggattg gtatggcagt
    10081 tattaaagaa cccgagcaat ttagagaaac caacgcattt aaggcatttt ggtttcattt
    10141 ccgacaagat aaacttgcac ttggcagttt ttatcttttt ttgctattac tggctctcat
    10201 ttttgttggt agtttgcttg ctccttacaa ttataatgaa caatttgttg gtttagaatt
    10261 aatgcctccc tcttgggacg acaaaggtca aatcagccat tttttaggca ctgacgattt
    10321 aggacgggat attttaagcc gtattatgta tggtttttac tacacgcttg gttccgcact
    10381 gatgattacc ttattaattg cgattatcgg cggtatgatc gggatttatg ccgggctgaa
    10441 aaaaggacgt tcattttggt tgattagcca tttgttcgat acctttttat ttatgccgat
    10501 tttattgatt gcgatcatca ttgcaacctt aatggaagcg agtttggtca atgcaatgtt
    10561 ggcgattttc ttagcgatgt tgccgcattt tatccataaa atttatcaag caaccgaaaa
    10621 acaactcaaa aaagagtaca tcattacctt aaagctagac ggtgcaagct gctggtattt
    10681 aattcgagaa gtgattattc ctaatcttac cggcgtggca attaaagcac tttcacatat
    10741 ttttgtgatc gccatattag atattagtgc gttaagtttt attatgctgg gggctaaaag
    10801 cccgacccct gaatggggag cgatgattcg tgaatccatg gaactgattt atatagctcc
    10861 ttggacggtg atattacctg gcttagcaat tatttttagt atttttaccg ttacaatact
    10921 tggtacacgc ttaagcgtga tttttgaaaa ataccgtaaa gaatagtatt taaataggat
    10981 actgacacat aacaagcggt catttttaag taaaatttta cagattaagg aatagtatgg
    11041 cattacttga tattcgccat ttaagtattg aaattgatat gccaaaaggc agagtaaaaa
    11101 tggtggataa catcaacctg actgttgatg agggcgaaat ttgtggcttg gtgggagaat
    11161 ctggttcggg aaaaagcctc atagccaaag tgatttgcgg tatgattaaa gatgaatgga
    11221 ttgtgagtgc agatcgcttt cgctttgacg atattgaatt actgaaactt tcgccagcgg
    11281 aacgccgaaa attggtaggc gatcatattt caatggtatt ccaagatccg cttactagcc
    11341 ttgacccaag ccagcaaatt ggtaaacaac tgatgcaaac cattcaattt agagggaagt
    11401 ggtggcaacg atttgggtgg aaaaagaaaa aagcaatcga gctactacac cgtgttggta
    11461 ttcgtgatca tcaagatatt atgaaaagtt acccaagcga tattaccgaa ggcgaagctc
    11521 agaaagtgat gattgcaatg gcggtggcaa atcagccccg attattggtg gcagatgagc
    11581 cgactaatat tatggaatca accacgcagt tgcaggttta tcgcttgctt tccagtatga
    11641 atcagaattt gggaacctca atcttgctgg tgagtaatga catgcgtagc attcaaaatt
    11701 gggttgattc ttacaatgtg ctttattgtg gacaaaccgt tgaaattgcg gataaagaga
    11761 gcattcttga atcgtcttac cacccctata ctcgagtgtt actcaatagt tcgccggact
    11821 tttctcagcc tttagccttt aaaagtagct taaatacgct acgaggctca gtcccgatgt
    11881 tacaagaaat gccgattggt tgtcgtttag gccctcgttg cccttttgca caaaaaaaat
    11941 gtatcaacaa accgccatta gtgaaagtaa aacagcacga gtttgcctgc cattttccga
    12001 ttaactttag agaacaaaat ttgcttaaaa ataaaacgga aaatacgccg tttgtggtgg
    12061 ctgaaatagc ggaaaaagag gcgttttaat gtggttatta ttggctaagt gtaacgggta
    12121 acattaaagt atctaatacg aaactcaagg gtaaatcgat caccgcaagc accgcgaata
    12181 taccgccttc ttgcattact tggaaatctt ttattacgcc ggcataaaca cggtaatcct
    12241 gttcaaccaa gcttagtact gtgccacaag cggtgaggtt aaacatcaaa tagagtagaa
    12301 aaacgagttt acaagcggtc aatttcatca aaaaatctcc aaatacgctt ttattttacg
    12361 ctatttttat cgggttggcg taaaattcgt tttaaaaaca gctattttca ggcagaatgt
    12421 agccgaatcg acattaaata tactaagagg atcttatatg caaacttaca acattgcagt
    12481 gcttgccggt gacggcattg ggccggaagt gatggcagaa gctatcaaag tgcttgaagc
    12541 gactcaacaa aaatttggct ttaaactaaa ttttaatcaa tataacattg gtggagcagc
    12601 gattgatgct caaggtaaag cgttacccga aaatacctta aaaggctgtg aagaggcaga
    12661 tgcgattttg tttggctcgg taggtggccc gaaatggacg catttactac ctgatgagca
    12721 acctgaacga ggttcgctat taccactgcg taaacatttc tcgctattct gtaatttacg
    12781 ccctgctgca ttgtatcaag ggctggaaaa attctgccca ttacgtgccg atattgctgc
    12841 taaaggtttt gatatggtgg tggtgcgtga attaaccggc gggatttatt ttggacagcc
    12901 gaaaggccgt gatggtgaag gtgcgcaaac gagagcgttt gacaccgaag tatactacaa
    12961 atatgaaatc gagcgtatta cccgtgtggc gtttgaatct gcaatgaaac gtaaaaaaca
    13021 cgtaacctct gtggataaag ccaatgtgtt acaaagctcg attttatggc gtgaaaccgt
    13081 cacggaagta gctaaagagt tcccagaagt cacgcttgag catatgtata ttgataatgc
    13141 gacaatgcag ctaatcaaag cacctgaatc gttcgatatt ttattatgct caaacatctt
    13201 cggcgatatt atttccgatg aggctgcaat gattacaggt tctatgggca tgttgccttc
    13261 agccagcctc aatgagcaag gtttcggctt atatgaaccg gcaggcggtt cagcaccgga
    13321 tattgccggt aaaaatattg cgaatccgat tgcgcaaatt ttatcagccg caatgatgtt
    13381 gcgttatagc tttaacttag gtgatgctgc tgatgcgatt gaaacagcga tccaaaaagc
    13441 ccttgctgat ggctaccgta ccgctgattt agctgatgac agcaaaccgc tttctacctc
    13501 agaaatgggc gatattattg cgaaaaatat tctcgcttaa ggctagaagt taaatagcac
    13561 aagcggtcat tattcgccaa atttttgcaa attttccgct aataatgacc gcttgtttat
    13621 tactcgttag ccgtcaatct cttcgcctgt ttcttcccac ttaaaattca atgccgcaga
    13681 gttgaggcaa aaacgcaacc ctgttggtgg tgggccgtcc ggaaaaacgt gtcccatatg
    13741 cgaatcacaa ttcccacaac gaatttcgat acgatgacgt cctaaactgt aatcatctaa
    13801 gtaacgtagt gacgattctg aaatcgtttg gtaaaaactt ggccagccac aaccggcatc
    13861 aaatttagta tctgagtgaa ataaatcgtt atggcatctg gcacaacgat aaattcctac
    13921 ccgatgttca ttcaaaaatt ttccggtaaa cggacgttcc gtaccgtggt tgattaaaat
    13981 atcaacttgt tgttctgtca gggcgtgaat aggtttaatc atcagcaatt ctcctaagat
    14041 agtagcgaag tcattgtagc tttttttgac ctgaaaatcg ttataaattt caaaatttga
    14101 catagatcac aaaaaattta tctgttatat tggaagggtt cttttggctt gtcaggaaag
    14161 tgaaaagatg tgctataatt caagcatctt tagtgtagta cataaagaac aaattttgtt
    14221 taactttaat ataggtagaa aaatctatgg caattaaaat tggtattaac ggctttggcc
    14281 gtatcggtcg tatcgttttc cgtgcagcac aacaccgtga tgacatcgaa gtggtaggta
    14341 tcaacgactt aatcgatgtt gattatatgg cttatatgct gaaatatgat tcaactcacg
    14401 gtcgttttga cggtactgtt gaagttaaag acggtcaatt agtagttaac ggtaaagcga
    14461 tccgcgtaac agctgagcgt gacccggcta acttaaaatg gaatgagatc ggtgttgata
    14521 tcgcagttga agcaacaggt ttattcttag atgatgaaac tgctcgcaaa cacatcaccg
    14581 caggtgcgaa gaaagttgtt ttaaccggtc cgtctaaaga tgcaacacct atgttcgtaa
    14641 acggcgtaaa ctttgatact tacgcaggtc aagacatcgt gtctaacgct tcttgtacaa
    14701 ctaactgttt agcaccatta gcgaaagtta ttcacgaaaa attcggtatc aaagaaggtt
    14761 taatgacaac tgttcacgca actactgcaa cgcaaaaaac agtagatggt ccatcagcga
    14821 aagactggcg tggtggtcgt ggcgcgtcac aaaacatcat tccttcatca acaggtgcag
    14881 cgaaagcagt aggtaaggta ttacctgcat taaacggtaa attaaccggt atggctttcc
    14941 gtgttccaac cactaacgtt tctgttgttg atttaactgt aaacttagaa aaaccagcaa
    15001 cttatgcaga aatctgtgct gaaatcaaac gtgcttcaga aaacgaaatg aaaggcgtat
    15061 taggctacac agaagatgca gttgtttcaa cagacttcaa tggtgcaact gaaacttcag
    15121 tatttgatgc agcagcaggt atcgcattaa ctgatacttt cgttaaatta gtatcttggt
    15181 acgataacga agttggctac tcaaacaaag tattagactt agttgctcac gtatataact
    15241 acaaa
//
LOCUS       DANLXQ010000049        15145 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.049, whole genome shotgun sequence.
ACCESSION   DANLXQ010000049 DANLXQ010000000
VERSION     DANLXQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15145
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.049"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1076)
                     /locus_tag="PXD94_001139"
     CDS             complement(<1..1076)
                     /locus_tag="PXD94_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HDL6286014.1"
                     /translation="MMSFLSEVFSSFKPTKAGVISSEPNENTGESDIIQNKMSEVNNE
                     VQQLKRSGKLTQSQHHRRLYSQEPSFIDHLPWGEYLNEHGVILLDDARSVGAVFDIKP
                     IGTAGRSIEFLSRIRNLVKDALQDSFDELDINPYVVQFYCQDSDDLQPYIEQLRNYIS
                     PIAQNSAFSEEWLRVQAKHFKDIGKPEGLFIDDRVTNTAWSGRIRSTKMVIYRYVDKS
                     NDSNENLIIEQLNNACSRINGALTTAGLQLQRLDGEQIRQWLLRWFNPNPSINFEGLS
                     NNDCYHLFGKQTSNDGLPELAFNDFSEDLFYSQPKNEGNYWYFDDQPHEVVVVNNLRK
                     QPEVGLMTGEITRGRNINALFDLLP"
     gene            complement(1076..1474)
                     /locus_tag="PXD94_001140"
     CDS             complement(1076..1474)
                     /locus_tag="PXD94_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL6286015.1"
                     /translation="MKHLFCIMISALLITGCTTSTEELLPTNGSTMRDIWDKGASNND
                     LHLYRSQQGRTLDPVDYIPNSEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            complement(1487..2941)
                     /locus_tag="PXD94_001141"
     CDS             complement(1487..2941)
                     /locus_tag="PXD94_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286016.1"
                     /translation="MKANKALFVILGIVFILVGGIVYALFGRSESVPQPTQQEILDIS
                     IKDLTPEQVRTMGIQGDTPIDTLRTLVTTVKSNERRMDELIDENRQLKQKEDLVNGRI
                     NDAVNNETRKLQEQIADLKMQLFDMATLVNNKPTTQESTPQVEAQPNTVPNKGFGTAG
                     DKLPIGGATMSNVDTSGMYWVEPSDQISLDASGKPVSGNTVATKFGFPKDFNGGQSAG
                     NNEKKGLPFFPETDTVTSSQGTAGNARGKNQMTSVYTIPSNSTFLDSVAMTGLIGRIP
                     IDNRVSDAYPFKVLIGRENLIANGIELPDVDGAIVSGTATGDWTLSCVRGSVKSMTFV
                     FNDGRIVSTSNKGNGEEIGWLSDSSGIPCVPGERKTNAPEYLGTNFFLAAASAAAQGF
                     SQSQTTTVVDGSSVVGAVTGDQGKFILGQAIGGGLKESADWFRKRFAENFDAVYVPPG
                     HRVSIHIEKEIEIDYNAQGRKVKYGSKSTNSQLD"
     gene            complement(2953..3837)
                     /locus_tag="PXD94_001142"
     CDS             complement(2953..3837)
                     /locus_tag="PXD94_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286017.1"
                     /translation="MKRLPILLVSLLIGFSGSSLADVLMKWERKPLPVALQVEKERII
                     FVDKNVKVGYPAELDGKLRIQSTGGAVYLKALETFPSTRIEFRDVTTGEIFLFDLTAA
                     SKVANPEEAIRVVFDGTVEKQSFSSNRDDESSDLPIANSRPQLPVPAALTRYAAQSLY
                     APLRTVEPLDGVRRVAHRLPKKITKLLPAYSIEATPLISWQLDEYVVTAIRLQNKGNT
                     QITLDPRELQGQFYAATFQHNWLGGYGENEDTTTLYLVTEGYANNALIPETRPVKLVS
                     KKVKRTTKVETKPAQSAK"
     gene            complement(3837..4481)
                     /locus_tag="PXD94_001143"
     CDS             complement(3837..4481)
                     /locus_tag="PXD94_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286018.1"
                     /translation="MSQLKSQLHEKDKHIKTTRIFAFLFFIIILVLAGVIYTFPSRLT
                     IYNPPDLRAGSQRAWWEIPKPTIYAFSYQIFQQMNRWMTNGEEDYEKNITTLKPFLTP
                     HCEAFLRQDYKDRLRNNELRSRTRMVYEITGRGYSEDKVTIFSEDSWAVNLDLSVDEH
                     FQGQPVKRVYIRFPISIVRMDVDSAQNPWGLGFNCYHSIPKLLEGVEPKTEEKK"
     gene            complement(4485..4850)
                     /locus_tag="PXD94_001144"
     CDS             complement(4485..4850)
                     /locus_tag="PXD94_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HDL6286019.1"
                     /translation="MSEQDTTIPFLPTRLNREATVYGGMTVSEFGISAGLGFMLGLIV
                     GLFLWILTDFWLLIPAMAMLLCIATVLIGKGIVAAVKRGKPEAYLNRLVEKKMDDLFN
                     GHKFIKREGFWSIRRYRRK"
     gene            complement(4873..5262)
                     /locus_tag="PXD94_001145"
     CDS             complement(4873..5262)
                     /locus_tag="PXD94_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2976 domain-containing protein"
                     /protein_id="HDL6286020.1"
                     /translation="MNRIKSLTKHLHSRYLAVMLFFWGSLQNAFAAGPDASKIPDFQI
                     PGVDSNTKDPMEIIWIVLKGLVTLGAVILAAVAILTVVKALIGIYNEATSENGKKGWG
                     HFILALIIGFIVIFFCLWLVKLAVGLF"
     gene            complement(5277..5510)
                     /locus_tag="PXD94_001146"
     CDS             complement(5277..5510)
                     /locus_tag="PXD94_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286021.1"
                     /translation="MSYSPANAFSVASGIDPFRLKIMIAIIFLSLLLLAVAWALNSGY
                     KGFSFRDKSPFSLLVLFLKGVALLACVTYFFIY"
     gene            complement(5510..5836)
                     /locus_tag="PXD94_001147"
     CDS             complement(5510..5836)
                     /locus_tag="PXD94_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HDL6286022.1"
                     /translation="MSISTSLRKGIVVLVTLSCLPALASTEQEQLAQAIKQLEAAKLS
                     LQRAEKVAKASPKTREYFNYSAIHSDIETVKKGIQQYINPSRALPRDPNVLRTLSDDY
                     SKLRGQ"
     gene            6023..6406
                     /locus_tag="PXD94_001148"
     CDS             6023..6406
                     /locus_tag="PXD94_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286023.1"
                     /translation="MRYQMRYLALFLVVFPYVAHSLPSAQKVGNICHAENAQYNYGGL
                     WELSLRGKLICNYWQFLGDDERKKIELQLDEYGEFPIWNTSEQISEQIIEDDEYPQYW
                     EVEDWEDSDWDTESENEDTEATTEE"
     gene            complement(6438..7133)
                     /locus_tag="PXD94_001149"
     CDS             complement(6438..7133)
                     /locus_tag="PXD94_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDL6286024.1"
                     /translation="MSEETKARRKSGLINLIFGKINMSLAAIAVSLIFSIILEWIGIA
                     FWWPQEGHLHSQTMMLNEMQWLSDDFSQSLFAKSSTAFATSIIENVYNWLFVYTGVKE
                     WLTSPGNGSMEQWVHHYGRAYIESVIYVVITFIIRLIIIVFTSPLFLLTALSGLTEGL
                     SLRDIRKFGAGRESAYLYHHARRYIMPMMVTAWVLYLSIPFSIHPNIILVPAACLFGL
                     SICITAASFKKFL"
     gene            complement(7130..7483)
                     /locus_tag="PXD94_001150"
     CDS             complement(7130..7483)
                     /locus_tag="PXD94_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6286025.1"
                     /translation="MSSAKMREEKRTNLLDLPNKYRNFNGEFSASCGLDNAEELLIHS
                     QSYFIEWFEQGYSFHQFAEKFADQGLSLWSADEVSMRHSDKSKDIFAFYLAFDNNPSG
                     YILVQCQLDREDSLQ"
     gene            complement(7476..9677)
                     /gene="traD"
                     /locus_tag="PXD94_001151"
     CDS             complement(7476..9677)
                     /gene="traD"
                     /locus_tag="PXD94_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDL6286026.1"
                     /translation="MSAKHTLEAWLRPPVEFFPAAVHASCAFVSAAAPWSLALHPSIG
                     YSIAAGFGALSFYRAKQGWQIVRYHRNLKRLPNYSMTSKQIPVSNKYLFVGKGFEWKP
                     IHTQRLHDCFSDNGSKYLEPSKLFKMAREYEKRHENWLTHLTSKDSRWNLVRPLPPVE
                     GIPAIHGIEPDEVDVVQSVSSRVGHTVVFGTTRVGKTRLAEVFVTQDIHRGKTADERE
                     VVVFFDPKGDADMLKRMYAEAKRAGREDEFYVFHLGYPEISARYNPVGRFNRISEVAG
                     RISGQLSGAGNSAAFKEFAWRFVNIVARALVELGNRPNYVQISQFVQNIDSLFLDYAE
                     KFIRKTDPKIWENLATIAAGLDEKALPFGMKDRPLIFVINKYIAESAVFDPILQGLAS
                     AVRYDRTYFDKIVASLLPLLEKLTTGKISELLSPDYADILDERPIFDWESIIRKRGIV
                     YIGLDALSDATVAAAVGNSMFADLVSMAGHLYKFGTNDGLPGAFKEKSKPVKINLHCD
                     EFNELMGDEFIPLINKGGGAGIQVTAYTQTLSDIEARIGSKPKAGQTLGNFNTLYMLR
                     VKEPATAELLTKQLHEVTIKRSTVTSSTTDSSNPDDDKAFSSNTGQRISESTVPMLVP
                     ANITNLPKGQAFALMDGSKLWKIRMPLPAVDKDDMMHQSLRELTDYMRKNYHVVENWW
                     QPAFTEYTPSRDIAEAFEAMSEPQYKTQIDHQVDNFGIDEPYDIDEEENDE"
     gene            complement(9677..10183)
                     /locus_tag="PXD94_001152"
     CDS             complement(9677..10183)
                     /locus_tag="PXD94_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6286027.1"
                     /translation="MQNKVKVLLFSLYVSHFSWADLTVIADFGGESAVRFYESLQPEH
                     DENAPNYPNSISASVSEADILPIVSHKLSPGTIKEKPFDLTGMQAIFLIGADNTSAQW
                     LQQNREKLITLNATGLVVNVQTLSELNQLRDIVPELRILPTPGDDLAERIGIAHYPVL
                     ITESGVSQ"
     gene            complement(10186..10956)
                     /locus_tag="PXD94_001153"
     CDS             complement(10186..10956)
                     /locus_tag="PXD94_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL6286028.1"
                     /translation="MDITRVLVIVAVFLSNISISQAKPFNGFGSEIDSYIRMAAQRYQ
                     VSEAMLRGFVKMEDGWYGNISPTGATGVGQFTMGTWNWLSETERGKQLGMKLITAKNR
                     GSYADPRRDKYTNTLATALLARWHIEQFQLRNIPITDENLYMAHNIGLDGLHRAMMGK
                     ATKEDILNMRRNGMKSWMTVRDFLNYQKGRFLAHKQEANFMTVSKQGHIHTGVAAFVY
                     PTSQMGKSVKWVEPDNKMIWVEPKNAMVWVEPQNTLQR"
     gene            complement(10935..11708)
                     /locus_tag="PXD94_001154"
     CDS             complement(10935..11708)
                     /locus_tag="PXD94_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286029.1"
                     /translation="MREISMKFNLCRKAIPLAVLPLLLSFALPTLAQNMQTETVSSSQ
                     STLQKQSQHQQSQLSDSLKQAKEWGLTVEEWSRYTELMKGERGMWSPNLDPLTALGIE
                     ARTAEEREKYARMLAKKYYERVSKELDFDKVYRQEFEKLYPNELPFEVEPHISQSVGR
                     VVYFTRFDNCDSCKSDLGKILSYANNQTPLDIYIVGQNISDNDIRKWAADNNIDPIKV
                     SKRLITLNHDKGYWLRYSLGKMPAAYQIKQDGEWTSLVY"
     gene            complement(11708..12337)
                     /locus_tag="PXD94_001155"
     CDS             complement(11708..12337)
                     /locus_tag="PXD94_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286030.1"
                     /translation="MKTKISLMTVFMLGLSGCVHQQDTAQNSDEITPMPDSLLTPQMQ
                     QLPPQDRIVSKDIYQEQTTSNTEVVRDGRYTLISIAPEEGQKYLLEQVVSVKVSNPKN
                     RFTANVQQGLRSTLTNTGLDLCYPPIGEQGNAVQTLFTRPLPKVHYQFGPMKLYEALQ
                     MIAGPAYELTLNDISRTICFKARESSFQNPHKPAVAVTTTTIKTEVIEE"
     gene            complement(12496..12756)
                     /locus_tag="PXD94_001156"
     CDS             complement(12496..12756)
                     /locus_tag="PXD94_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286031.1"
                     /translation="MTFELGRVCTTRAVAECFNQNALVELLNRYCYGDWGNLCEEDKL
                     ANQEAMKYGGRIVARYDVDDESLYIITEADRSCTTILFSYEY"
     gene            complement(12869..13231)
                     /locus_tag="PXD94_001157"
     CDS             complement(12869..13231)
                     /locus_tag="PXD94_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286032.1"
                     /translation="MEDSFFDFDDISCYLGQWEAILEEYSDIVSIEDFWLVAKEFETV
                     PHFGNLYQELVISRLIQRFCTELDIEQDSDLVEFDYYINAIDTHFYINRQRICDIDDW
                     NEMLDKIRKEMTPAKLAA"
     gene            complement(13311..13718)
                     /locus_tag="PXD94_001158"
     CDS             complement(13311..13718)
                     /locus_tag="PXD94_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL6286033.1"
                     /translation="MNRQGIYINGKQVFDSKGKEINSVQIINSTVQTVVGINKGYISM
                     AAGNNVLIVDGQTIALEPSPTINIEVFGNVTNLNAGSENVRVTGDAKSVSTVSGDVTV
                     DGNVTSNVKTVSADIHCKSILGKINTVSGDIYR"
     gene            complement(13744..14424)
                     /locus_tag="PXD94_001159"
     CDS             complement(13744..14424)
                     /locus_tag="PXD94_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286034.1"
                     /translation="MNSLQQFVKTLSPTVLNGRHFDGIVGDVVFVQNGYGKWLDQGFT
                     IIGFDKSIEEKDWIYLDWDCYWFGKNPAQVIPEKSWAQRSWLARAYSISLERFNSLGL
                     EREYNIVSDWDNPIYGTDIQLIEHSKEFFRWTEADDRGIFLVHNEMAFHFFKKEIKAS
                     SEPEYYQPIETFWKDATVVLNIEDLDMLERVGLEQTNTPALAKLTTFVQAARNEISNG
                     NIVFYRAF"
     gene            complement(14530..14961)
                     /locus_tag="PXD94_001160"
     CDS             complement(14530..14961)
                     /locus_tag="PXD94_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL6286035.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEHKKGNPFYACRVAAL
                     VGESDSPEYRYFDMNVVGKETETLIKKCQKAVSEEKKVLVSFVMADLWFDTFTYSSDS
                     KYHKKGDTGVSLKGRLIRIKMIKIDGEIVYQEKPKADEQSE"
BASE COUNT         4493 a         3139 c         2877 g         4636 t
ORIGIN      
        1 ggcaataggt caaataacgc attaatatta cgcccacgag taatctcgcc ggtcattaag
       61 cccacttctg gttgttttct taaattattg actacaacaa cttcgtgcgg ttggtcatca
      121 aaataccaat agttgccttc atttttcggt tgagaataaa ataaatcttc actgaaatcg
      181 ttaaacgcta attctggaag cccgtcattt gaggtttgtt tgccaaaaag atggtaacaa
      241 tcattgttgc ttaatccctc aaaattaata gatggattag gattaaacca acgcaataac
      301 cattggcgaa tttgttcacc atctagccgc tgtaactgta atccagccgt tgttagagcc
      361 ccattgattc gggaacaagc attattaagc tgctcaataa taagattttc attggaatca
      421 ttggacttat ctacatatcg ataaatcacc attttggtac tccgaatacg tccagaccaa
      481 gctgtatttg tcactctgtc atcaataaaa agcccttctg gtttaccaat atccttgaaa
      541 tgcttggctt gtactcgtaa ccattcttcg ctaaatgcac tattttgggc aataggcgag
      601 atataattcc gtagctgttc aatataaggc tgtaaatcat cgctatcttg gcaataaaat
      661 tgcacgacgt aaggatttat atccagctca tcaaagctat cttgtaaggc atctttgaca
      721 agattacgta tccgacttaa aaattcaata ctacggccag cagtaccaat cggcttaata
      781 tcaaatactg ccccaacact acgggcatca tcaaggagta tcacaccgtg ctcattcaaa
      841 tattctcccc aaggtagatg atcgataaag gagggctctt gtgagtataa ccgacggtga
      901 tgctgtgact gggtgagttt tccactgcgt tttaattgtt gaacctcgtt atttacctca
      961 ctcattttat tttgaatgat gtcagactca ccggtatttt catttggttc acttgatata
     1021 acccctgctt tagtcggttt gaaagaggaa aatacctcac ttaaaaaaga catgattaaa
     1081 gccctcttgt tctatcaccc ggctgagcat attgaactcg tccatagaaa ggtagtactg
     1141 tagaatatcc tggaatactg agaggctctc cggaacgagt ttgatgtggg tacacataca
     1201 taatcagatc cggattaggt aaacgaggga acaagttatt aatttcattt tctgccgtac
     1261 gggtataggc tcgttgctca ctattaggaa tataatcgac agggtcaagt gttcgccctt
     1321 gttgtgaacg gtataagtgt agatcgttat tactcgctcc tttatcccaa atatcccgca
     1381 tcgtactgcc gttagtaggt aataattctt ctgttgatgt tgtgcaacct gttatcaata
     1441 aggcacttat cattatgcaa aataaatgtt tcatcgtttg ttccttctaa tccagttgtg
     1501 aattcgttga ttttgagcca tatttcactt tacgaccttg ggcgttgtaa tcgatttcaa
     1561 tctctttctc gatatgaata gagacacgat gacccggtgg cacatataca gcatcaaagt
     1621 tttcagcaaa acgttttcta aaccagtcag cagattcttt taagccgcct ccgatagctt
     1681 gtcctaagat gaacttaccc tgatcgccgg taacagctcc aacaacacta cttccatcaa
     1741 caacagtggt tgtttgtgac tgtgagaatc cttgggcagc agcacttgca gcagccaaaa
     1801 agaaattcgt tcccaaatat tctggggcat tggttttccg ctctcctgga acacaaggga
     1861 tacccgaact atctgataac cagccaatct cttcgccatt ccctttatta gaggtactca
     1921 caatacgtcc atcattgaac acaaaagtca tactttttac actgcctcta acacaggata
     1981 gtgtccagtc gccagtagca gtacctgata caattgctcc gtctacatca ggtaattcaa
     2041 taccattagc aatgagattt tctcgaccaa taagtacttt gaacggataa gcatccgata
     2101 ctcgattatc aatcgggata cgtccgatga gtcccgtcat tgcaactgaa tcaaggaatg
     2161 ttgaattact tggtatggta tatactgatg tcatctggtt tttaccacgt gcgtttccag
     2221 ccgttccttg tgagcttgtc acagtatctg tttccgggaa aaacggcaga cctttctttt
     2281 cgttatttcc tgcagattgt cctccattaa aatctttcgg aaagccaaat ttagttgcta
     2341 ctgtattgcc tgacacaggt tttccacttg catccaaact aatctgatcg cttggttcta
     2401 cccaatacat tccactggta tccacgttgg acatagttgc accaccgata ggtaatttat
     2461 ctcctgctgt tccaaatcct ttattcggta ccgtattagg ctgagcctct acctgagggg
     2521 tagactcttg tgttgttggt ttgttattaa cgagagtcgc catatcaaat aattgcattt
     2581 tcaaatctgc aatctgctct tgcagttttc gggtttcatt atttaccgca tcattgatac
     2641 gaccattcac taaatcttct ttctgtttga gttggcgatt ttcatcaatt aactcatcca
     2701 tacgacgctc attggattta acggttgtca caagggtacg cagcgtatca atcggcgtat
     2761 caccttgaat acccattgtt ctgacttgtt caggtgttaa atctttaatg gaaatatcta
     2821 aaatttcctg ttgtgtcggt tgtggaactg actcactacg tccaaataag gcatagacaa
     2881 ttccaccgac taagataaat acaatgccca gtatgacaaa taaggcttta ttcgctttca
     2941 ttgcctttcc tcctacttcg ccgattgagc cggtttagtt tcaacttttg ttgttctttt
     3001 cactttttta ctgactagtt tgactggacg ggtttccgga attaatgcgt tattagcata
     3061 gccttcggtc actaaataca gggttgtggt atcttcattt tctccgtacc ccccaagcca
     3121 attatgctgg aaagtcgctg cataaaattg tccttgaagc tctcgagggt caagcgtaat
     3181 ttgagtgtta cccttatttt gcaaacgaat agcggtaact acatattcgt ctaactgcca
     3241 gctaattaaa ggtgttgctt ctattgaata agccggtagc agcttggtaa tttttttagg
     3301 tagacgatgt gccacacgac gcacaccatc taaaggttca actgtacgta gtggagcata
     3361 aagtgattgt gctgcataac gggtcaatgc ggcaggtact ggaagctgag gacggctatt
     3421 cgcaataggc agatcagagc tttcatcatc tctattgctt gaaaaagatt gtttttctac
     3481 cgtaccgtca aaaaccactc gaattgcctc ttcaggatta gcgactttcg aagctgctgt
     3541 taaatcaaat agaaaaattt caccggttgt aacatcacgg aattctatgc gagtggaggg
     3601 aaatgtttct aatgctttta aatagacggc tccaccggta gattgaattc gtagcttacc
     3661 gtccagttct gccggataac caaccttgac atttttatcg acaaaaataa ttcgttcttt
     3721 ttccacttgt aacgcaacag gaagtggttt tctttcccat ttcatcaata catcagccaa
     3781 gctactccca gagaagccta taagcaaaga gacgagcaaa attggtagac gtttcattat
     3841 tttttctcct ctgtttttgg ctcaacacct tctaagagtt ttggaatgga gtgatagcaa
     3901 ttaaacccta atccccaagg gttttgagca gaatcgacat ccattctcac aatactgata
     3961 ggaaaacgaa tataaactcg ttttaccggc tgtccttgga aatgttcatc cacacttaaa
     4021 tcaagattga cagcccaact atcttcactg aatatggtaa ctttatcttc actatatcct
     4081 ctaccggtaa tttcatacac catacgggtt cggctgcgta gttcattgtt acgcagacga
     4141 tctttataat cttgtcgtaa aaatgcttca cagtgtggtg ttaaaaatgg tttgagggtg
     4201 gtgatatttt tctcataatc ctcttcgcca tttgtcatcc accgattcat ttgctggaaa
     4261 atttgatagg agaatgcata aattgttggt ttaggaattt cccaccaagc tcgttggctc
     4321 ccagcacgca gatctggtgg gttgtatatc gtaaggcgac ttgggaaagt atagattacg
     4381 cctgccagta ccaaaataat aatgaaaaac aggaaggcaa aaattcgagt cgttttgata
     4441 tgtttatctt tttcgtgtaa ttggcttttt aactggctca tttgttattt ccttctatat
     4501 cggcgaatag accaaaaccc ttctcgtttt ataaacttat gaccgttaaa gaggtcatcc
     4561 attttctttt caactaatct atttaaataa gcctctggtt ttcctcgttt aaccgcagcg
     4621 acaatacctt ttccgattaa gacagttgcg atgcagagca acattgccat tgccggaata
     4681 agaagccaaa aatcagttaa tatccacaag aagagaccaa caatcagacc taacataaat
     4741 cctaatccgg cagatatgcc gaactcacta actgtcatcc caccgtaaac ggtagcttca
     4801 cgattaaggc gggtgggaag aaaaggaatg gttgtatctt gttctgacat attggtctcc
     4861 tagacatttt aattagaaga gtccgactgc aagtttgact aaccacaagc agaagaaaat
     4921 cacaataaag ccgataatta aggcgagaat aaagtgtccc caaccttttt taccattttc
     4981 agatgtagct tcgttataga tgccaatcaa tgctttaaca actgtcaaaa tggctacggc
     5041 tgctaaaata acagctccga gagtgacaag acctttcaga acaatccaaa taatttccat
     5101 tggatctttg gtattactat ccacgcctgg aatttggaag tctgggattt tggaagcatc
     5161 aggaccggct gcaaatgcat tttggaggga accccagaag aagagcatta cagccaaata
     5221 acgactatgc agatgtttcg taagagattt aatgcggttc ataatagacc tcgaatctaa
     5281 tagataaaga aataagtaac acaagctaac agtgccactc ctttgagaaa taaaactaaa
     5341 agtgaaaaag gacttttatc tctaaatgaa aatcccttat aaccactatt caatgcccaa
     5401 gcgacggcta agagcagtaa tgaaagaaaa ataatggcga tcatgatttt caatctaaaa
     5461 gggtcgatac cgctggcaac agaaaacgca ttcgcagggg agtaagacat cactgccccc
     5521 gtaatttgct ataatcatca cttaatgtac gtagtacatt cggatcacga ggtaatgctc
     5581 ggcttggatt gatatattgt tgaattcctt ttttgacggt ttcaatatca ctgtgaatag
     5641 cagaatagtt aaagtattct cgtgttttag gcgaggcttt agctactttt tctgctcgtt
     5701 gaagtgataa ttttgccgct tccagttgct taattgcctg ggctaattgc tcttgttctg
     5761 ttgaagcaag tgctggcaag caagataagg taacaaggac aacaatacct ttccttaaag
     5821 atgttgaaat agacatagtg cacctctatt tttagtttta cttcaggtta agagtgccaa
     5881 aagcgatcct aaaatttaat ggaaaactct gttaccgttc agaagagaaa ttgcacaaaa
     5941 tagtaaatgg tgagaaagat attattctat gtttaagcta gtcaattaag tagtatcaac
     6001 accgaaatct tcacgcttta cgatgagata ccagatgaga tatctagcac tgttccttgt
     6061 agttttccca tatgttgccc attctcttcc ttcagcacag aaagtgggga atatttgcca
     6121 tgcagaaaat gctcaatata attacggagg tctttgggaa ttaagtttaa gaggaaagtt
     6181 aatctgtaat tattggcaat ttctaggcga tgatgagcga aaaaagatag agttacaatt
     6241 ggatgagtac ggggaatttc ctatttggaa tacctctgaa caaatttctg aacaaatcat
     6301 tgaagatgat gaatatccac aatattggga agtcgaagac tgggaggatt ctgattggga
     6361 tactgagtca gaaaatgaag atacagaggc aacaacagaa gaataagccc ccgcatattg
     6421 ctaagggctt atgaaattta caagaatttc ttgaaactcg ccgcagttat acagatggat
     6481 aaaccaaaca aacaggctgc tgggactaaa atgatgttcg gatgaattga aaacgggata
     6541 gataaataaa gcacccaagc tgtgaccatc attggcataa tataacgtct agcgtgatgg
     6601 tataggtagg ctgattcccg cccggcacca aattttcgta tatctcgtaa tgacagtcct
     6661 tcggttaaac cggaaagtgc agtcaataaa aagagtgggc tggtaaaaac aataataatg
     6721 aggcgaataa taaaggtgat caccacataa ataacagatt caatataggc tctgccgtag
     6781 tgatgtaccc actgttccat actaccatta cccggtgaag ttaaccactc cttaacaccc
     6841 gtataaacga ataaccagtt atacacgttt tcaataattg atgttgcaaa agcagtagaa
     6901 gacttggcaa acaggctttg agaaaagtca tccgatagcc attgcatctc attgagcatc
     6961 atcgtttggc tatgcaaatg cccttcttgc ggccaccaga aagcaatgcc tatccactct
     7021 aaaataatgc tgaatatgag agatactgca attgcagcaa gcgacatatt tatttttcca
     7081 aaaatcaggt tgattaaacc tgattttcgg cgagctttag tttcttcact cattgcaaac
     7141 tatcctctct atcgagctgg cactggacaa ggatatagcc ggaaggatta ttatcaaaag
     7201 caagataaaa agcgaaaata tctttggatt tatccgagtg acgcatagat acttcatcgg
     7261 cagaccataa cgaaagccct tgatctgcga atttttcggc aaactgatgg aaggaataac
     7321 cttgttcaaa ccattcaata aaataggatt gactatgaat caggagttct tctgcattat
     7381 ctaagccaca ggaagctgaa aactccccat taaaattacg gtatttattt ggtaaatcta
     7441 atagattggt tcttttttcc tctctcattt tggcactact catcattttc ctcttcatca
     7501 atatcgtaag gttcatctat gccgaaatta tctacttgat ggtctatctg tgttttatat
     7561 tgaggctccg acatcgcttc aaatgcttct gcaatgtctc gggacggcgt atattctgta
     7621 aatgccggtt gccaccaatt ttcaacaacg tgatagtttt tacgcatata atctgtcagc
     7681 tctcgtaaag attgatgcat catatcatct ttatcgacag caggtaacgg cattcgaatt
     7741 ttccataatt tactaccgtc cattaaagcg aatgcttgtc cttttggtaa attggtaatg
     7801 ttggccggaa ccaacatagg aacggtactt tcgctgatac gctgtccggt attggagcta
     7861 aacgccttat catcgtcagg attactggag tcagttgtac tggacgtgac cgtgctacgt
     7921 ttaatcgtca cttcgtgtaa ctgttttgtg agtaattctg ccgtagccgg ttcttttacc
     7981 ctcagcatat agagtgtatt gaagttacct aaggtttgtc ccgctttcgg tttactgccg
     8041 atacgagctt caatgtcgga gagtgtttgt gtataagcag ttacttgtat tccagcacca
     8101 ccgcctttgt tgatgagagg gataaattca tcacccatca attcattaaa ttcatcacaa
     8161 tgaagattaa ttttcactgg tttggatttt tctttgaatg ctcccggtaa tccgtcattt
     8221 gtcccaaatt tatataaatg acctgccatt gagactaagt cagcaaacat actgtttcct
     8281 acagcggccg caactgtcgc atcagagaga gcatctaaac caatataaac gattcctcgt
     8341 ttacgaataa tggattccca gtcaaaaatc ggacgttcat ccaaaatatc agcataatcc
     8401 ggggataaca gctcagaaat tttccctgtc gttaattttt ccaataacgg caacaatgag
     8461 gcaacaattt tatcgaaata ggtacggtca tagcgtacag cactggcaag tccttgtaga
     8521 attgggtcaa agactgcact ttcagcaatg tacttgttga tcacaaaaat cagcggacgg
     8581 tctttcattc cgaaaggaag tgctttttca tccagtccag cggcgatggt tgctaaattt
     8641 tcccaaattt ttggatcggt tttacgaatg aatttttccg cataatcaag gaataatgaa
     8701 tcaatatttt gtacaaactg ggaaatttga acatagtttg ggcgatttcc caattcaact
     8761 aatgcacgtg cgacgatatt gacaaatcgc caagcgaact ctttaaatgc cgccgagtta
     8821 cccgctccac ttagctgtcc tgaaatacgc ccagcgactt ccgaaatacg attaaagcgt
     8881 cctactgggt tataacgggc agaaatttcg ggataaccta gatggaaaac ataaaactca
     8941 tcttctcgcc ccgctcgttt tgcctccgca tacattcgtt tgagcatatc ggcatcgcct
     9001 ttcggatcaa agaagactac cacttcacgc tcatcagcag ttttacctcg gtggatatcc
     9061 tgggtaacaa agacctcagc taaacgagtt ttaccgacac gtgttgtacc gaatactacc
     9121 gtatgcccaa cccgagagct aacggattga acgacatcaa cctcatccgg ctcaattcca
     9181 tggatagcag gaataccctc aacaggtggc agaggtctaa ccagattcca ccggctatcc
     9241 tttgaggtaa gatgtgttag ccaattttcg tgtcgttttt catactcacg agccatttta
     9301 aatagcttag aaggctccag atattttgaa ccgttatccg aaaaacaatc gtgtaaccgt
     9361 tgagtgtgaa taggtttcca ttcaaatccc ttaccgacaa acagatactt attactgacc
     9421 ggaatttgct tgcttgtcat tgaataatta ggcaaacgtt tcaggttgcg atggtaacgc
     9481 acaatttgcc aaccttgttt tgcccgataa aagctcaatg ctccaaaacc agctgcaata
     9541 ctgtaaccga tacttggatg gagtgccaaa gaccaaggag ctgcagcact gacaaaggca
     9601 caagaagcgt gaaccgctgc cggaaaaaat tcaacaggag gtcgtaacca cgcctctaag
     9661 gtatgtttag cagacattat tgtgaaactc cgctttcagt gattaagaca gggtaatgtg
     9721 caataccaat tcgctctgca aggtcatctc cgggagttgg taatattctc agttccggta
     9781 caatgtctcg taattgatta agttcggaga gtgtttgtac attcactaca agccctgttg
     9841 cattcaacgt gatcaacttc tctcgatttt gttgtaacca ctgagctgag gtattatctg
     9901 ctccaattaa gaaaatggct tgcattccgg ttaaatcaaa tggtttttct ttaatagtcc
     9961 cgggagatag tttatgggaa acaatgggta aaatatcagc ctcactcact gaggctgaaa
    10021 tggaattcgg atagtttggt gcattttcat cgtgttccgg ttgcaaagat tcataaaaac
    10081 gtaccgcact ttcaccaccg aaatcagcaa tcacggttaa atcagcccaa gagaaatggg
    10141 acacatataa actaaacaat aatactttta ccttgttttg catttttacc tctgaagagt
    10201 attttggggt tctacccaaa ccattgcatt tttaggttca acccaaatca ttttattatc
    10261 cggctcaacc catttgaccg attttcccat ttggcttgtg ggataaacga aagctgcaac
    10321 tccggtatga atatgccctt gcttggagac agtcataaaa ttggcttctt gcttatgtgc
    10381 taaaaaacgt cctttttggt aattgagaaa gtctcttacc gtcatccaac ttttcattcc
    10441 attacgacgc atatttaaaa tgtcttcttt agtagctttc cccatcattg ctcgatgcag
    10501 cccatctaag ccgatattat gagccatata taaattctca tcagtaatag ggatattacg
    10561 gagttgaaat tgctcaatat gccaacgtgc caataatgcc gttgctaagg tattcgtata
    10621 tttatctcga cggggatcgg cgtaagaacc ccgatttttg gctgtaatca acttcatacc
    10681 aagttgcttt cccctctctg tttcagatag ccagttccag gttcccatag taaactgccc
    10741 aacaccggtt gctcctgttg gtgaaatgtt gccataccag ccatcttcca ttttgacaaa
    10801 acctcgtagc attgcttcac tcacctgata acgttgagca gccatacgaa tatagctgtc
    10861 aatttcagaa ccaaatccat tgaagggctt tgcctgagaa atgctaatgt tggaaagaaa
    10921 gactgcaaca atgactaata cacgagtgat gtccattcac catcctgttt gatttggtaa
    10981 gcagcgggca tttttcctaa cgaatatctc agccaatagc ctttatcgtg gttaagggta
    11041 atcaatcttt tactgacttt tattgggtca atattattgt cggcagccca tttacgaata
    11101 tcattatcgg agatgttttg cccaacaata taaatatcga gaggtgtttg attatttgcg
    11161 taagagagga ttttcccaag atcactttta caagaatcgc agttatcaaa acgggtaaaa
    11221 taaaccacac gacctaccga ttgagaaata tgtggttcaa cctcaaaagg tagctcattc
    11281 ggataaagtt tctcaaactc ctgacgataa actttgtcaa aatcaagttc ttttgacact
    11341 cgttcataat attttttcgc caacatacga gcatattttt ccctttcctc agcagttcgt
    11401 gcttcgatac ctaaagcagt taacggatct agatttggcg accacatacc tcgctcacct
    11461 ttcattaatt cggtataacg actccattct tcaacggtca atccccattc tttagcctgt
    11521 tttaggctat cacttaattg tgattgttga tgttggcttt gtttctgcaa agtcgattgt
    11581 gagctactta cggtctctgt ttgcatattt tgagccagag taggtaatgc aaaggacaat
    11641 aaaagaggta gtactgccaa cggtatagct ttacggcata aattaaactt cattgatatt
    11701 tccctcatta ttcctcaatg acctcggttt taattgttgt cgttgtaacg gcaacagcag
    11761 gtttatgagg gttttggaat gaactttccc gagctttaaa gcaaatcgta cgggatatat
    11821 cattaagagt tagttcataa gcgggacctg caatcatttg tagagcttca tataatttca
    11881 tcggaccgaa ttggtaatga acttttggaa gaggtcgggt aaaaagtgtt tgaaccgcat
    11941 taccttgttc tccgataggt ggataacaca aatctaaacc cgtattagtt aatgtagaac
    12001 gcaaaccttg ctggacattc gcagtaaaac gatttttagg attggagact ttgactgata
    12061 cgacttgttc aagcagatat ttttgacctt cctccggagc aatactgatt aaggtgtagc
    12121 gtccgtcacg taccacttcg gtattcgagg tagtctgttc ttggtaaata tctttggaca
    12181 cgatacggtc ttgtggaggt aattgttgca tttgaggtgt tagcaatgaa tcaggcatag
    12241 gtgtgatttc atcactattt tgagctgtat cctgttgatg aacacaacca cttaagccaa
    12301 gcataaaaac agtcattaag gatatttttg ttttcattta atctctccaa gtatgaaagg
    12361 aatctagacc aactatggcg aagtcattgc tgattttgaa tgatttattc tgttgttgat
    12421 tgagagaata aaaaaacgcc atcattagac ggcgtttgaa aacacaataa tgtgctgtgt
    12481 taatcggatt tctccttagt attcataaga aaataagatt gttgtacagc tacgatcagc
    12541 ttctgtgatg atataaagac tttcatcatc tacatcgtag cgagctacaa ttctccctcc
    12601 gtatttcatt gcttcctgat tcgctaattt atcttcctca cacaaattgc cccaatcacc
    12661 ataacagtaa cgattaagca attcaaccaa ggcattttga ttgaaacatt cagcaacagc
    12721 acgagttgta catactcgac caagttcaaa cgtcatacat ttctcctaaa taaaatacgg
    12781 ggaaatgtca cccattaggg gagtatttcc ccaagtgggt ggactaagac tgcggcaaga
    12841 gtcgttttta gtaaaaattt gcaaaaaatt aggcagctaa tttagccggc gtcatttctt
    12901 tacgaatctt gtctaacatt tcattccaat catctatatc acagattctt tggcgattaa
    12961 tgtaaaaatg cgtgtctatt gcattgatat agtagtcaaa ttcaactaaa tctgaatctt
    13021 gctcaatatc taattcagta cagaatcgtt gaattaagcg agagatgaca agctcctgat
    13081 acagattacc gaaatgtggt acagtttcaa attctttggc aacaagccaa aagtcttcaa
    13141 tactgacaat atcgctatat tcttctaaga tggcttccca ttgtcctaaa tagcaagaga
    13201 tatcatcaaa gtcaaaaaag gaatcttcca ttatgtttct ccaagtttta aataaaaagt
    13261 ggggaaacat acctaccgag aatgtttccc gatagggtgt atagtcaata ttagcgataa
    13321 atatcaccgc tcactgtgtt aattttacct aaaatagatt tacaatgaat gtcagcactc
    13381 acagttttca cattcgaggt tacattacca tccactgtga catcaccact gacagttgaa
    13441 acagatttgg catcgcctgt tacacgaaca ttttctgaac ctgcattgag gttggttaca
    13501 ttgccaaata cttcaatgtt gatagtcgga gagggttcta atgcaattgt ttgtccatca
    13561 acaattaata cattattgcc ggcagccatt gagatataac ctttatttat cccaacaact
    13621 gtttgcactg tactattaat gatttgaacg ctgttgatct ctttgccttt agagtcaaag
    13681 acttgtttac cgttgatata aataccttgt cgattcatat aataatcctc tttgtattag
    13741 atgttaaaat gctcgataga atacgatatt accattggaa atttcgttgc gagcagcctg
    13801 tacaaacgta gttaattttg ctaaagcagg tgtattagtt tgctctaatc ctactcgctc
    13861 taacatatct agatcttcta tgttcaatac caccgtagca tctttccaga atgtctcaat
    13921 aggttgatag tattccggtt ccgatgaggc tttaatttct tttttgaaga aatgaaaagc
    13981 catctcgttg tgaactaaga aaatccctct atcatcggct tcagtccaac ggaaaaactc
    14041 cttggaatgt tcaattaact gaatatctgt accataaatg gggttatccc aatcagacac
    14101 aatgttatat tcacgctcta agcctaacga attaaaacgt tcaagagaga ttgaatacgc
    14161 tctagcaagc caagaacgtt gagcccaaga tttttcaggg attacctgtg ccggattttt
    14221 tccgaaccaa tagcaatccc aatctaggta aatccaatcc ttttcctcta tgcttttgtc
    14281 aaaaccaata atagtaaagc cttgatcaag ccatttacca tagccgttct gaacaaagac
    14341 aacatcgcca acaataccat caaaatggcg accattaagg actgttggtg ataatgtttt
    14401 gacaaattgt tgaagtgagt tcatcgtgtt tctcctaaaa ttgtaaaata aaggaaaaca
    14461 caccgcaggg agtgtttccc tacgggacat aaagtaaagc ccaacataaa ttgtcgggct
    14521 tgggtaaaat tactcagatt gctcatctgc ttttggtttt tcttgataga cgatttcccc
    14581 atcaatttta atcattttga tgcggattaa gcgaccttta agagacactc cagtatcgcc
    14641 ttttttgtga tatttactgt cacttgaata agtgaaagta tcgaaccaca aatcagccat
    14701 tacaaatgat acaagcactt ttttctcttc agagaccgct ttttggcatt ttttgatgag
    14761 tgtttcggtt tctttaccga ctacattcat atcaaaatat ctgtactctg gtgagtcaga
    14821 ttcgccaaca agagctgcaa cacgacaagc ataaaaagga tttccttttt tatgctctac
    14881 taaacgaata tcgtttaaat agccaatgcc ggtagtatgt agattgaagt aatttttttg
    14941 agtagaagtt tgagttgtca tagtttgttt ctccaaatgt aaaaaagcgg agaaacatcc
    15001 ttacccaaca cgggaaaaat gttccccgca aggtggaagt aaaatggaat ctcaatgaat
    15061 attcactgaa atgataggga ttgagttggc attcctgcaa atttacagag ttccctttca
    15121 gaatgctggg agacattttg gcaat
//
LOCUS       DANLXQ010000050        14905 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.050, whole genome shotgun sequence.
ACCESSION   DANLXQ010000050 DANLXQ010000000
VERSION     DANLXQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14905
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.050"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            15..233
                     /locus_tag="PXD94_001161"
     CDS             15..233
                     /locus_tag="PXD94_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6286036.1"
                     /translation="MWLRELFLKRRLELGLTQRTLGEKMGVLYSFIGKVETGDRRLDI
                     FEFIAYCKGLDLDPLSVLQEIEQQFPHK"
     gene            complement(280..900)
                     /locus_tag="PXD94_001162"
     CDS             complement(280..900)
                     /locus_tag="PXD94_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL6286037.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(902..1555)
                     /locus_tag="PXD94_001163"
     CDS             complement(902..1555)
                     /locus_tag="PXD94_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL6286038.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            1604..2263
                     /locus_tag="PXD94_001164"
     CDS             1604..2263
                     /locus_tag="PXD94_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL6286039.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            complement(2300..3346)
                     /locus_tag="PXD94_001165"
     CDS             complement(2300..3346)
                     /locus_tag="PXD94_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL6286040.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(3346..4299)
                     /gene="dusC"
                     /locus_tag="PXD94_001166"
     CDS             complement(3346..4299)
                     /gene="dusC"
                     /locus_tag="PXD94_001166"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL6286041.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            complement(4377..5003)
                     /locus_tag="PXD94_001167"
     CDS             complement(4377..5003)
                     /locus_tag="PXD94_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL6286042.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            complement(5003..5860)
                     /gene="djlA"
                     /locus_tag="PXD94_001168"
     CDS             complement(5003..5860)
                     /gene="djlA"
                     /locus_tag="PXD94_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL6286043.1"
                     /translation="MQFIGKIVGFFLGYQLFHGLFGGILGAFIGHLADKKLYELGSVR
                     SSLFGKNLTRQSLFTQTTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQ
                     NAFTLGKEPDFPLRQVIQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFT
                     IAETMGMSRFQFEQMIAMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIE
                     AAYTVLGVTAQDDQNTVKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYD
                     LICKVKEWK"
     gene            complement(5908..6618)
                     /locus_tag="PXD94_001169"
     CDS             complement(5908..6618)
                     /locus_tag="PXD94_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6286044.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVLTKKQSK"
     gene            complement(6854..7339)
                     /locus_tag="PXD94_001170"
     CDS             complement(6854..7339)
                     /locus_tag="PXD94_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL6286045.1"
                     /translation="MKKFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAANAA
                     GEFGGLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADER
                     GTPEYNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLE
                     Y"
     gene            complement(7366..8652)
                     /gene="tolB"
                     /locus_tag="PXD94_001171"
     CDS             complement(7366..8652)
                     /gene="tolB"
                     /locus_tag="PXD94_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL6286046.1"
                     /translation="MKLFSRISGVIGIFSALLTTQVVADSDVRISIDEGVSMAQPIAV
                     VPFKASGVPADVAQIISDDLRNSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIV
                     VGQVSSTGGGYNVAYQLIDTLGTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKL
                     TQIRGAFRTKIAYVVQRGVSSYELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAY
                     VTFENKKAQVVVHDIRSGARRVVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVG
                     SGGGTPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGS
                     YNAKVSSDGKNLIMIAGDRVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKG
                     TSKVLQLVSADGRFKANLPGAGGHYKFPAWSPYLTK"
     gene            complement(8668..9891)
                     /gene="tolA"
                     /locus_tag="PXD94_001172"
     CDS             complement(8668..9891)
                     /gene="tolA"
                     /locus_tag="PXD94_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL6286047.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQEQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     NMFKSPTLDFSLKVK"
     gene            complement(9905..10342)
                     /gene="tolR"
                     /locus_tag="PXD94_001173"
     CDS             complement(9905..10342)
                     /gene="tolR"
                     /locus_tag="PXD94_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL6286048.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(10484..11164)
                     /gene="tolQ"
                     /locus_tag="PXD94_001174"
     CDS             complement(10484..11164)
                     /gene="tolQ"
                     /locus_tag="PXD94_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HDL6286049.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGSTRAMNLALNREMESLENYIPFLGTVGSISPYIGLFGTVWGIMHSFMGLSAV
                     KQATLQSVAPGIAEALIATAIGLFAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLL
                     HRQAFSKR"
     gene            complement(11186..11587)
                     /gene="ybgC"
                     /locus_tag="PXD94_001175"
     CDS             complement(11186..11587)
                     /gene="ybgC"
                     /locus_tag="PXD94_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL6286050.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
     gene            complement(11750..12037)
                     /gene="ybgE"
                     /locus_tag="PXD94_001176"
     CDS             complement(11750..12037)
                     /gene="ybgE"
                     /locus_tag="PXD94_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDL6286051.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFLPLLGYLIVIPALWILLVK"
     gene            complement(12037..12135)
                     /locus_tag="PXD94_001177"
     CDS             complement(12037..12135)
                     /locus_tag="PXD94_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL6286052.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(12150..13283)
                     /gene="cydB"
                     /locus_tag="PXD94_001178"
     CDS             complement(12150..13283)
                     /gene="cydB"
                     /locus_tag="PXD94_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL6286053.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(13296..14846)
                     /locus_tag="PXD94_001179"
     CDS             complement(13296..14846)
                     /locus_tag="PXD94_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL6286054.1"
                     /translation="MLDVVELSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTG
                     KEVYKDMTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFN
                     FETMRMELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGRDLGFAKR
                     SFSVGASFGLIAIISVLIMGDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPN
                     TAEMKNEFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSG
                     NYTEADKEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRV
                     MMLSGFLMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQP
                     WAIYEMLPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGR
                     YYFEQSTK"
BASE COUNT         4526 a         3288 c         2653 g         4438 t
ORIGIN      
        1 attccagcga gcatatttgg ttaagagaac tattcttaaa acgccgtttg gaactagggc
       61 taacacaacg cacattagga gaaaaaatgg gcgttcttta ctcatttatc ggaaaagtag
      121 aaacaggaga tcgcagatta gatatttttg aatttatcgc ttattgcaaa gggttagatt
      181 tagatccttt atctgtattg caagaaattg aacaacaatt tcctcataaa taaaggcatt
      241 gagttataac tcaatgcctt tattttctat gtcaaatctc tacatgctcg gcttagtctc
      301 tgccagaatg gcgtatttat tattcatttc ctctgcccaa gcggttaaat ttgggtaatt
      361 ttttgcaaga tcgtagccta tcttatctaa gcggaatgcc caccagtcaa ttaatgctac
      421 tgcggttaat gtgcctaaat tcagctctgt gccaaactct tgcaatgcct cttctaattg
      481 tctgaacgaa cggatattgc gttccattaa ttgctcgtgg cggctgaccc accattcatt
      541 ctcggggcga gccatacgtt cttgtaataa agttggcatc gtattttcca taatgccgtc
      601 cgccaaatta tgtaatgcca acactgccca gcgaggttta ccctctttag gtaacagtgg
      661 agtgttattg cctttttggt caagatattc gctaattaac aaactaccga aaagccaacg
      721 tccgcagttg cgttgtaggg caggaatacg tcctaacggg ttatcttgat tatgtggcga
      781 atttggatcc attgcttttg tcacccgcaa catttcgact ttatcgtcta attggtggtg
      841 tttaatcacc gcaagggctt tgcgggcgaa agggctggtg gttgagtacc aaagtttcat
      901 actattctcc gtgtttgatg tgattggatt tgcaaatttt ttgccaaaaa tgaccgcttg
      961 tcggcaaggc ttgcactgag attttctcat tttcgtattc aaaatcaagc tgataagcgt
     1021 gcaaataaac acggtcggct tggctacctg aatagagttt atcacctaaa atcgggctgc
     1081 ccaaactttt cattgccact cttaattggt gagttttgcc tgttttgggt tgcaaaataa
     1141 aatgcctgag cgagtcttca acagaatgag aaataaattg cgttactgcc gggttttcct
     1201 tactgtggca taatttccac gctccatttc tgcttttttg catatcgcca acaattctac
     1261 cctgcttttt cttcggcttt ttatcgctta atgcccaata ggttttgtag attttatgct
     1321 gttcaaataa atggtaaaac cttgctgccg cctctttatt caacgctaaa atcagtaaac
     1381 ctgaggtgat tttatccaga cggtgaacca gccaaacctg ctttacattc agttggcgag
     1441 ccactaattc cgttaagccg atttcagcct catctttatg cacactcaag ccttccggct
     1501 tattgataat aataaaatca cgatgttgat aacaaatagg caatgttggg gtcattttgc
     1561 tataatcctt ccaatttttg actattttag caaggaaatt acaatggctg ttaccattta
     1621 tttagtacga cacggcagaa ccgtgtggaa tttggaaggc agattacaag gctcgggcga
     1681 ttccgcttta gtagaagaag ggattatcgg agcgaagaaa accggcattg cgttaaaaca
     1741 tattccgttt accgccgcct attccagtat gcagaaacgg gctcaagata ccgcaaacta
     1801 cattttagca gaaaatgaac gcagtaatat tccgcatttt catcataaag gcttgaatga
     1861 atttgatttt ggcagctggg aaggaatgaa atctgtcgat ttgcaagaaa atgatgaata
     1921 ttgggtgatg aaacgtacac cggcagaata tctggcaaaa gcaaacggtg gcgagcgatt
     1981 tgagcaactt tatcaacggg tgactcaggc gttcaatcaa atcgccgagc tacaccaaaa
     2041 tgacggcaaa gtgctgattg tttcacatgg tatgacctta accttactca cggctgtgtt
     2101 aaaaggcatt gcgtggcaag atttccgcaa tgaagaaaaa catagctttg tgttcaatac
     2161 tgccattacg aaagtagaag tagagaatgg caaagtaacg gtgctggaat ttaataatac
     2221 cgaccatttg gataatgtgc caacaagcca atacaaacga taatattaag ggcggaacat
     2281 tgtccgccca tagcattttc taatcagaga acactttttc tttcgtttct gttttagcaa
     2341 ataccgtatt tttgaccgaa gtaagcaact cctgacgaat agctgaaatt cttggcttct
     2401 cattcgcttg aaaagcaaca ggacgacaaa attccattgc tttaatgcca aggcgagcag
     2461 tgagtaaacc cacaccaata ccttgtgcgg cacggagcga taatttcgcc gtaaggtttt
     2521 gcgagaagaa atccattcca acatcagtag cgatttcggt tgcaccggcg aacaccatat
     2581 ttttcagcac cattttgaac agttttaagc ggctgatata acccaattcc attccatagg
     2641 ctttggtaat tttattcact aaagcgatat ttcttgccgc cattaacaac acatcaacta
     2701 ttgccagcgg gctaaccgcc acaatcaccg cattttctgc cgcattgcgg gaaatcatct
     2761 gcttaacctg tttatctatc ggctttagca cattttcgct aaacagatat agtacctctt
     2821 tggcattata agcctcgttc agttcgcttt tccaaagttg ctcggcttga gcaatgctcg
     2881 gcaagtgatt aagctgattt gcaatatttt cgcaaaattt gaccgcttgt tcgccatcat
     2941 tggaaggcag ctgttccagc aataattggt tactcatttg ttgatttttc tgatgatttc
     3001 gcaaatacac caactttcgc cattcggaaa taatcgcccc aacgcccaac aagctgacaa
     3061 taaaaaagac tatcgagaac gcaaaataga tccactctct ttgctggaac gtatcaatca
     3121 accactgaat actttgagca attaccgcca cgccaaacaa ggcaagtgtg gcaagtagta
     3181 ctcgaatcca aaagcgggaa ggcttaaggc tttcttcaat aatcagctcc gcctcaactg
     3241 ctttttcctc ttcttcaata atcgcatttt cgagatcaaa ctcttgttta ggttcaaaat
     3301 gctcggttgt ttttagctct tcttcattaa aaatttgctt actcattatt cctccactgc
     3361 cttttcaata tgtgctttta gaccatcata gccgtgttcg gttttcaaga tatcaaataa
     3421 ttctaccgct tgaggatatt ctttatccaa ataacgtagc cattgtttga tgcgagcaac
     3481 gtggtaaaaa ccggtatcaa actgattttc catctgaacg tacttaaaca atagctgtag
     3541 cacttcattc cacgccattt tcggaacatt atatttcacc actttgctta aattcggtgt
     3601 gtttaacgcc ccacgcccca tcattaatgc cttgcaagcg gttatttttt ggcaattttt
     3661 cgccgattca aaatcccaaa tttcgccgtt ggcgataaca ggaatagaca gccgttgttg
     3721 aatttcgcca atcgcctgcc aattaattct ttctgctcga taaccatcaa ctttggttcg
     3781 cccgtgaacg gtaatttcat tcgctccgcc ctgttctacc gcatcggcaa tttcaaaaca
     3841 ttggctaacg gaatcccaac ctaatcgcac ttttacggaa acgattttat cgcttggcac
     3901 agcttcacgc atcgctttgg ttgcacgata aatcagttcc ggatctttta ataacgaagc
     3961 cccaccattg ctgccattta ccgttttaga ggggcagcca cagttgagat ctaccccgtg
     4021 tgaaccaagc tcaatggcaa gagctgcatt ttctgccagc cactgtgggt gttgccctaa
     4081 aagctgtacc cgcactggtg tgccggaatc ggttaatccg ccttgatata gctcaggggc
     4141 taggcgataa aaggttttct tcggtaaacg ttgatcgacc actcgcacaa attcggaaat
     4201 acagagatcg tagtcattca ctgaagttaa aaggttgcgg acaaaagggt ctaaaacccc
     4261 ttgcatcggg gcaagaataa cgttaggaat tggattcatt ggtaaaataa aaaatacaag
     4321 cggttatttt ttctcaacat tttgcaaaaa attggcaaga aacaaccgct tgaaggttaa
     4381 aaatgtctat cgtgaatgcg caaatcgggg cgagcgatca ggtaaatcat cagtgcggtt
     4441 aatgccccaa gagtatccgc aagcatatct ttttgagcgt cccaaatatc cccttgcgag
     4501 cctagaaatt ccacgccggc attgccacct tcaatcacgg catattgcca ttcaataatt
     4561 tcgtaagctg ccgccaccga cataatgaaa aacagcgaga agaaaaatgc tacgcccatt
     4621 ccgcacttgc gtttacgcag taaccattct gccatcggga agctgtaaaa accgataatg
     4681 taatgtgcta ctcgatcaaa atggttacga tcttcaccta aaatcggggt tagaaattga
     4741 tttgcccact caaacggcac attggcaaag gtatattttg ccccgatcaa gtgcataatc
     4801 agccaaagcg acatcaaaaa ataagctaac ccactaaact gaaatttagg gtaagtgata
     4861 attaaaaggg caaaaacgat aaaaatcggt atcacttccg cataccaaac tgcgcggtct
     4921 gtcggattta accctgacca aaccgcaatc acggtaataa caatcgctaa aaatagaggg
     4981 atgatatgcg agcgtgaatc cattatttcc actctttcac tttacaaatt aaatcgtacg
     5041 ccgcttgaat ttgttgagct ttctcttttg ccaactccat catctcatca ggcaaacctt
     5101 tagcggctaa tttatccggg tgatgttcgt tcattaactt acgataagcc cgtttcactg
     5161 tattttgatc gtcttgtgcg gttacgccta atacggtata ggctgcctca atgcttggtt
     5221 gtgcaggctg ctgataactg ccctgatagc caccttgaga ttgctgatga taactcccat
     5281 tttgctgata atagccacca ttgcggaatt gttgagctgc cataatcatt gcgatcatct
     5341 gctcaaattg gaagcgagac attcccattg tttcggcaat cgtaaacaga atttgttgct
     5401 cgttcacatc aagttgccca tcttgcaagg ctgcctgcat ttgcacttcg acaaagaaac
     5461 gtaataagtc agttcttgcc ccacaggctt ctctgaactc ttgaatgact tggcgtaacg
     5521 ggaagtcagg ctctttacct aaggtaaagg cattttgcgc taattgctgg gctgcactat
     5581 ctaattttaa gcgagccatt aacgctcggg ctaattcaat atccgcttca gtaacacgcc
     5641 cttttgcttt cgcaatatgc cccaacaccg caaaagtggt ttgggtgaaa agcgattggc
     5701 gagttaaatt tttaccgaaa agacttgaac gaactgaacc taattcatag agttttttat
     5761 cggctaaatg cccgataaaa gcgccaagta tcccaccaaa taaaccgtga aaaagctgat
     5821 agcccaagaa aaaaccgact atttttccga taaattgcat ttttttatcc ttttatagaa
     5881 atgaataaac gttattctaa caaaatttta ctttgattgc tttttagtta atacaaacat
     5941 aagccagctt gagacaaaaa taattgcata acctagccaa ctcaacatat ccgggtattg
     6001 ttcaaagaac aacaacccta aaattgcact aaatagcaca gaggcataac tataaattga
     6061 gatgtctttc gccgccgcat atttataggc ggtagttaca caaaactgcc cgaccgctga
     6121 agttacccct gctaacacta agtagattgt ctgtaaattg gttagcacta cataatcaat
     6181 ggcgacaaaa ggcaataata tcaaggtcga aaaggcagaa aaaaagaaaa caatggtatt
     6241 aggtgattct acttttttgg tttttgccaa taaccttaag cataaatagg caatggctgc
     6301 aaacacagag ccaatcacgc caaccagata aggcataaag cgaatatcaa ctgccggttt
     6361 gataataaaa agtgccccga taaaggcaat aaggcataaa atccattgca ggagagatgc
     6421 tctctctttt aaaaaaatcg cagaaagaat aattaaaatg aacgacgtga gtttgccaat
     6481 catatcgaca tcacttagca caaggtgatc gatgatatat acaccacata aaatccccaa
     6541 taacccggtg atggaacggc ttaataataa caaacgattt tctgcataac caaagaataa
     6601 ttggcgattt ttccacacaa tataaccgct tatcaatgct gtgcgttgcg aaaaatcgct
     6661 ttttgtgcaa tcggtacatc ccccgaaaga cgaagaaaag cagccataat cgcaaagctt
     6721 agtgccgaca aaagcacaca aaaaatggct ttaaataacg gcgagagcga attccaaaaa
     6781 ttcatagcat aaaggctttt aataaaaata aagccctgat tattcagggc ttcaatttag
     6841 aatcaactaa gaattagtat tctaatactg cacgacggtt tttcgcgtag tcagcttctg
     6901 agtgacctaa tactgctggt ttctcttcac cgtaagaaac agttgaagtg ttgttagcac
     6961 ctttagctgc taagtaacct ttaactgcat ctgcgcgacg ttgacctaat gcgatgttgt
     7021 actctggagt accacgctca tcagcgtgac cagcaatagt tacgttacct tgagtttttg
     7081 ctaataattg agcgtgagcg tctaataatt gacggtattc accttcaact gcatagctgt
     7141 caaaaccgaa gtatacagtg ttgtattgtg cttttaattg ttcagctgtt aaaccaccga
     7201 attcgcctgc tgcatttgct gctgcatttg ctgctgcgtt tgctgctgcg tttgctgctg
     7261 catcatctga tgagctgcta catgctgcta atgcgaaagc tggtactgct accattaata
     7321 ctttagcaaa ttttttcatt tgttgctcct aatagagaaa tattattatt tagttaagta
     7381 tggtgaccat gcagggaatt tataatgtcc acctgcgcca ggcaaattag ctttaaaacg
     7441 cccatctgca gacaccaatt gtagcacttt actcgtacct ttggtagagc tataaataac
     7501 cataatgcca ttcggtgaaa tacttggact ttcgtctaag aaagttgaac tcagtacttc
     7561 tgtgccacct gaagcgagat cgcgtttcac aactctatca ccggcaatca tgattaaatt
     7621 cttaccatca gatgatactt tcgcattata gctaccgcta ccgcccattg ggctagcacc
     7681 accacctgat gaactcattc tataaacttg aggagaacct gcacggtcag acgtaaagta
     7741 gattgtgcta ccatctggag accagcttgg ttcagtatta tttccagcat tagcggtcaa
     7801 tttacttggt gtaccaccgc cactacctac aacataaata tttaactcac catcttgatt
     7861 tgaggcaaac gcaatgcgag aaccgtctgg agagaatgat ggcgcaccat tatgtccttt
     7921 caatgctgcc actacacggc gagcacctga acgaatatcg tgaacaacaa cctgcgcttt
     7981 tttattttca aatgtcacat aagctaatct gctaccatct ggtgaccact ctggcgacat
     8041 taaaggctct ttactggttg taatagtaaa ggcattaaaa ccatcataat cagatactcg
     8101 taattcatac gatgatacgc cacgttgaac cacatacgca attttagttc taaatgcacc
     8161 gcgaatttgg gttaattttt caaatacctc atcacttact gtatgcgcac cttgacgaat
     8221 ttgggctgcc ggaacaataa atgaaccttg ggcaatgaca ccacccgctg tacccgatgt
     8281 accaagagta tcaattaatt gataagcgac attataaccg ccacctgtgc tactcacttg
     8341 gccaactaca atattatcaa taccgatagc actccattga tctgccacca cttccgctgc
     8401 tgatcccggt tgagcaggca tttgagagcg ttcaagcggt gtaaatttac cactatttcg
     8461 caaatcatca gaaataattt gtgcaacatc tgccggcaca ccgctggctt taaatggcac
     8521 aaccgcaatc ggctgagcca tacttacacc ttcatcaata gaaattcgca catcagaatc
     8581 agcaaccacc tgagttgtca ataatgctga aaagatgcct attacacccg atatacgaga
     8641 gaataatttc atttgtagtc ctctaaatta tttaactttt aaactaaaat caagtgttgg
     8701 tgatttaaac atattatata ccgcatctga tggtgcaggt ggtacttttc tagttgccac
     8761 tattgcactt accgctgcat cacatactgc tttatcgcca ctgataactt gatagttagt
     8821 aattgtgcca tctcgactta agaatatctt aacatcacat tgttttccgg caaaacttgg
     8881 atcaacccga tatcgagatt gaattaattt cttaattcgt tgaccatatg cattaccatc
     8941 tacattagta ccttgcccta agccacttgt accaccagaa ccttgtgatc ctgctgagtt
     9001 tttgttacca cctttacttg aaccaccacc gatgtctcca ctattgagga aatcatcaag
     9061 tgctttattg tttttcgctt gggcggcttt tgcctcagca gctgccttcg ctttagcttc
     9121 tttctcggct ttctctttgg cttctttttc tgcttttgct ttggcttctt tctcagcctt
     9181 ttcttttgct tctttttctg ccttggcttt agcttctttt tctgcttttt ccttcgcttc
     9241 tttagcttct tttgcctgct gttccttcag ttctttttgc tcttttaact ttttctcagc
     9301 ttccgctttc ttctgctgtt caagcttctt ttcaagtgct tctttagctt ttttctcttc
     9361 ttcagcttgt ttagcagcag cctctaaacg cttagcttct gcatctgctt tcagttttgc
     9421 tgcttcagct gcttttttac gagttgcttc ttctgcttgc tgctttttaa gctcttcttg
     9481 cttagcttgt tcttgctttt gctgttcttg tagtttttgc tgacgctcaa tttcttgctg
     9541 acgctgtttc tcttgaatcg ccgctaactc agcttgtttc tgtttttgga tctcttccgg
     9601 agtcggttta tcttccacat cttcttcaac agcttcctct ttaggctcta cctttggctt
     9661 cgatgctttt gagccttttt tctctgccaa taactgccca tattcagcag ccacttgccc
     9721 agtatctacc atcacagctt caaagctatc gctatcgcca ccactgccac cagccgcagc
     9781 gagttccgtc ttagtgaaaa gagacccgag caataacaac ccgattaaca atatatgcaa
     9841 taagatcgaa acaataaccg ctagctctag tctatcttgt tgagatttca cacgttatcc
     9901 ttggctattt accttctgtc attaaaccta ccgatttcac tccggcgtct ttaagtagtg
     9961 taataccttt cataatttct tcataaggta ctactttatc accggcaact aaaaataagg
    10021 tattgttatc tttttgaaat tgttcccctg cataggcaac aacttctgat tcattaatat
    10081 tctcttgccc attactttgc acatagttac cgtcaatttt caatttatat tcaccaacac
    10141 ctgccacctc caaaatcact ggagttttat cttcattaga aactgattgg ctatgcgtat
    10201 cttccggtaa attaacttct acactttgac tgataattgg ggccgttgcc ataaaaatca
    10261 gcaataatac caacaataca tcaagaaatg gaacaatgtt gatttctgat ttaatgtcat
    10321 tacgttttaa acgacgagac atatattcct ctttacaggt gcttagcacc ttaattgatc
    10381 tgaaataacc ggcacagtgt cccggtctcg aataatgcgg ttacaagcgg tcttttttac
    10441 gaaaaacttt acaaattttc attaaaaatc aaccgcttgt agattatctt tttgaaaaag
    10501 cctgacggtg taataaggtc gtgaactcat caatgaaatt cacataccct tgctcaactt
    10561 tacctaaacg taaattcaaa cggttatatg ccattactgc aggaatcgcc gcaaataaac
    10621 cgatagccgt tgcaattaat gcttctgcaa tacccggtgc gactgattgt aaagttgctt
    10681 gcttaactgc acttaatccc ataaaggagt gcataattcc ccaaaccgtt ccgaataaac
    10741 caatatatgg gctgattgaa cccactgtgc ctaaaaacgg aatgtagttt tctaaacttt
    10801 ccatttcacg gtttaatgct aagttcatcg cacgggtaga accttgaata atcgattctg
    10861 gcgcatctgg gttcgcttgt tgtaaacggg taaattcttt aaacccaaca taaaagattt
    10921 gctctgaacc atttaatgca tcacgacgat tctctaaacc ttggtgtaaa cggtttagat
    10981 cttcccctga ccagaatcga tcttcaaaag caagggaatc ttttttggct ttagttaaaa
    11041 ttttgctgcg ctgaataatc accgcccaag acaaaattga gaatacaata aggatcagca
    11101 tcacgatctt taccacaata cttgcttcta aaaataacga aagaagacta aattcagttt
    11161 gcattaataa aactccgaat agtttttaaa tagaaaaata acttttttgt aatgcttgac
    11221 gaatatcatc aggaattgca accggcttca ttttgattaa atcaacacaa gcgaccgtaa
    11281 catttgcctc actcagacaa tcgccatctt tccacaatcg ttgtttaaag ataagggttg
    11341 ctttcttcaa ttctgagagt gttgtttcca catttaataa atcatctaaa cgagcaggga
    11401 gcttataatc tatttcaatc ctcttaacaa caaaagcaag ggattggcta aaaagttcct
    11461 gctgtgagaa atgctgctgt ctcaaaaatt ccgttcttgc ccgctcaaaa aaattcagat
    11521 agttggaatg ataaactact ccgccggcat cagtatcttc ataataaaca cgaatcggga
    11581 aatccataag cattatttct ctcattcaaa atgaaaaaac gcccaaaatt taggcgcaaa
    11641 ttgtgagata ttttaggatc agaaatattg ttatgcaagt atattttttg gtaaattgca
    11701 aaaatatttt ttatctatca aaaataaaaa tagagacata aaaatgcctc tatttaacaa
    11761 gtaaaatcca taatgccgga ataacaataa ggtaacctag aagtggtaaa aaaatcactt
    11821 tccaaatagc agctcggatt tcaaatccaa ttccatgtac ccataaaata gccataccat
    11881 aaaataccaa cattgctaaa tacggcgttt ttccaccaaa agaaagagcg aaagcggtcg
    11941 aatttaccca aatagttgca aataatacag ccgctaaaat aaaagaaagg gcttttaaca
    12001 agccctttgc tgttaagtta tagagtttat gaatcattac tcgtcaaact tacctgtttt
    12061 ttcaatgctg atcgtgatga ctgttgcaaa gaagattgca agcaatacac ctaagaccca
    12121 agttacataa aacatattgt gtctccttat tagtaagctg ctttattttc ttctaaataa
    12181 ctagcatcta aacgaccata cattttgata tatgaccaaa tagtatatgc taatactaac
    12241 ggtacaaaga taccagcaac aaataacatt acagttaatg ttgtgtggct agcagtagcg
    12301 tcccaaaccg ttaagctcat ttctggatga gtaattgacg gcataatgaa tgggaacatt
    12361 gatactgcag cagttaaaat caccccaaca atcatcaatg atgatgagaa gaatgctaaa
    12421 cctgaacgat ttgcttttga ggcaataata gtcagtaatg cacctactac aactaatgca
    12481 ggaacaaccc ataatacagg catttcagca taatttctaa accatgcacc agcttgaacc
    12541 gctacagttt tattcgtcaa tagagattgt gcattatgat ctaactcgct tgtgatcaca
    12601 aaaccatctt taaaatataa ccatacacct gcaagaataa atgccactaa tacaatagaa
    12661 gctgtcactt gtgccacttt tcttgagttg ttatgtagct catcagtagt tttcatttgt
    12721 aaccaagttg caccctgtgt ggttaacatc attaagctga taataccgca cactaatgca
    12781 aatggattta ataaaccaaa gaatgaaccc gtatattgtg attggttaat attattaaat
    12841 tcaaatggta caccttgtaa taagttaccg aatgcaacac cgaatactaa tgatggaacg
    12901 aaaccaccaa taaataatcc ccaatcccaa gcactacgcc atttcggatt atcaattttt
    12961 gcacgatact caaaacctac agggcggaaa aataatgctg caagcacaag caccattgca
    13021 atatagaaac ccgagaacga agttgcatag actgttggcc atgcagcgaa gatcgcacca
    13081 ccagcggtca ataaccatac ttggttacca tcccagtgag gggcaattgt gttgatcata
    13141 atacgacgtt caacattact tttaccaatg ataggtaaaa ggtttaatac gcccatatca
    13201 aaaccatcag taattgcaaa gccaattaac aataccgcaa ttaagatcca ccaaataaag
    13261 cggagaattt cataatcgat catttctaac tcctattatt tagttgattg ttcaaaataa
    13321 taacggcctg tttttaatgc actagggcca agacgaccat atttgaacat taaatacatt
    13381 tctacaacta agaatagtgt atataaacca cacaataagc cgattgtaaa ccataaatca
    13441 cctgttgtta atgcagagtt tgacacaccg acaggtaaca tttcatagat agcccaaggc
    13501 tgacggccat attcggcaag gaaccaacca gattcaatcg caatccaagg taacggaaga
    13561 ccgaataaca agcctcttaa taagaaacgg ctactttcta ctttaccacg catattttga
    13621 ataaatgctg tcgtaatcaa gataagcatc aagaagccag ataacatcat tacacggaat
    13681 gcccagaatg tagggcctac atttggaatt gtattatctg ctgctttttg aatttgttct
    13741 tcagtcgcat caaccacatt aggtgtatat tgttttaaca ataacccaaa gcctaaatct
    13801 ttttgtacag ctttaaatgc ttctttatcc gcctctgtat aattgccaga acgtaatttt
    13861 tctaatagtc cgtaggctac cataccggta cgaacacgtt gttcattaat tacacgaata
    13921 tcttttaggc ctttaatttc tttatctaaa gaacgggttg caataatacc agctgcataa
    13981 ggaatatgaa ttgagaattc atttttcatt tccgcagtat ttggaatcac aaaagcattc
    14041 caagcagctg gagcaggctg agtttcgaat tcaccttcca ttgcagctaa tttagtcggc
    14101 tgagctttac cgatttcata accagattca tcacccataa tcagtacaga gatgatcgca
    14161 attaaaccaa aacttgcacc tactgagaat gaacgttttg caaagcctaa gtcacgacct
    14221 tttaagatat agtatgaact aatacctaat acaaaaatag caccacaaac atagcctgcg
    14281 gttacagtat gtaagaattt agattgtgta actgggttta atacaagttc agagaaactt
    14341 gaaagctcca ttcgcatggt ttcaaagttg aattcagaag caatcgggtt ttgcatccaa
    14401 ccattagcaa ctaaaatcca tagtgcagag aagttagaac caaacgcaac acagtaagtt
    14461 gctaataaat gtttcgcttt cgataaacgt tcccagccaa agaagaacaa gcccacaaag
    14521 gtagattcta agaagaacgc cattaaacct tcaatcgcta atggagcgcc aaaaatatca
    14581 cctacataat gagaataata tgaccagttc ataccaaact ggaattccat tgtaatacca
    14641 gttgttaccc ctagggcaaa gttaatacca aataatttac cccagaattt agtcatatct
    14701 ttatatactt ctttacccgt agttacatac agggtttcca tcaccacaag gacgaaagaa
    14761 agacctaatg taagcggtac gaataagaag tgatagagtg cagttagagc aaactgcaaa
    14821 cgtgagagtt caactacatc taacatctca aacctcttat attaatatac taatttgaca
    14881 attagattac tgtaaaagta atcac
//
LOCUS       DANLXQ010000051        14813 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.051, whole genome shotgun sequence.
ACCESSION   DANLXQ010000051 DANLXQ010000000
VERSION     DANLXQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14813
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.051"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            166..597
                     /locus_tag="PXD94_001180"
     CDS             166..597
                     /locus_tag="PXD94_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL6286055.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEPKKGNPFYACRIAAL
                     VGESDSPEYRYFDMNVVGKETETLIKRCQKAVSEEKKVLVSFVMADLWFDTFTYSTDS
                     KFHKKGDTGVSVKGRLIRIKMIKIDGEIAYQEKPKSDEQSE"
     gene            615..1607
                     /locus_tag="PXD94_001181"
     CDS             615..1607
                     /locus_tag="PXD94_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6286056.1"
                     /translation="MLCWALLLDVQDLRVTFKTPDGDVTAVNDLTFSLNAGETLGIVG
                     ESGSGKSQTAFALMGLLADNGRISGSAKFNGQEILNLPETQLNRLRAEQIAMIFQDPM
                     TSLNPYMKIGEQLMEVLMLHKGMDHKTAFEESVRMLDAVKMPESRKRMNMYPHEFSGG
                     MRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKSEFNTAIIMITHDLG
                     VVAGICDNVLVMYVGRTMEYGTAEQIFYTPSHPYSIGLIGAVPRVDGQEEELITIPGN
                     PPNLLRLPSGCPFQPRCVHATKQCLKAPALTLLNDQRLRACFVPAEQLIRGNAS"
     gene            1604..2593
                     /gene="oppF"
                     /locus_tag="PXD94_001182"
     CDS             1604..2593
                     /gene="oppF"
                     /locus_tag="PXD94_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDL6286057.1"
                     /translation="MNTEKKVILEVKDLSVHFNIKDTKSLFFAKHQTLKAVNHVSFQL
                     YEGETLGVVGESGCGKSTLARAIIGLVESSGGNILWLGKELTQQSAKEWKTIRSDIQM
                     IFQDPLASLNPRMTIGSIIAEPLKIYQPHLTAEEVKEKVRAMMMKVGLLPNLINRYPH
                     EFSGGQCQRIGIARALIVEPKLIICDEPVSALDVSIQAQVVNLLKSLQREMGLSLIFI
                     AHDLAVVKHISDRVLVMYLGNAVELGDYEQVYHDTKHPYTKALMSAVPIPDPQLERNK
                     QIQLLEGDLPSPINPPSGCVFRTRCPLADQHCIADKPLLKGRDNHVVACFKAE"
     gene            complement(2697..3455)
                     /locus_tag="PXD94_001183"
                     /pseudo
     CDS             complement(2697..3455)
                     /locus_tag="PXD94_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(3585..4226)
                     /gene="bioD"
                     /locus_tag="PXD94_001184"
     CDS             complement(3585..4226)
                     /gene="bioD"
                     /locus_tag="PXD94_001184"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDL6286058.1"
                     /translation="MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGC
                     RGIAEDLLIHRKIQGIELTEEDKQGITCPYVFDYPCSPHLAAKLEPRTIERKKIETCT
                     ALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
                     VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEIMDLISV"
     gene            complement(4244..4999)
                     /gene="bioC"
                     /locus_tag="PXD94_001185"
     CDS             complement(4244..4999)
                     /gene="bioC"
                     /locus_tag="PXD94_001185"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDL6286059.1"
                     /translation="MDRIDKGRIKQRFSAAVSNYDQQARAQQQIHQRLLSLLSYIDRQ
                     AFQRALEIGCGTGGLTKQLMSQLQVEHWELNDLCDMREHLQSVLPQPFHFHCGDAEQL
                     PFPQQYDLIASASVVQWFEDKAGFIARCKVRLKKHGFLLFSTFTPENLTQIKHLTGVG
                     LTYPRLEDWQKWLVPEFEILALEHEEIVLHFDDPLAVLKHLKQTGVTATQQQRWTKGQ
                     LHAFCQQYQQHYRNAQHKVELTYTPMYVLARLT"
     gene            complement(5003..5650)
                     /locus_tag="PXD94_001186"
     CDS             complement(5003..5650)
                     /locus_tag="PXD94_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HDL6286060.1"
                     /translation="MKTQHISQQGDNLIVYFAGWGTPPSVVEHLILPENYDLCLCYDY
                     RDLHCDVDFSAYQHIRVVAWSMGVWVAERVMQGYPLLSATAINGTGLPCDDQYGIPEA
                     IFAGTLSGLNEDNRTKFERRICGSKTLLRDYQHYSMRPTLQEIHAELTALYTFLQQDH
                     RTDLISWSKAIVGSKDKIFPAQNQRAYWQDRCAITEIAGEHLLFPHFTHWETCWL"
     gene            complement(5647..6810)
                     /gene="bioF"
                     /locus_tag="PXD94_001187"
     CDS             complement(5647..6810)
                     /gene="bioF"
                     /locus_tag="PXD94_001187"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDL6286061.1"
                     /translation="MIRYYQQQLADLKAQNQFRQLPQLIHRGRFIQREDNTMLNMSSN
                     DYLGLANNEALRQAFFTQYQDQLPALTSSSSRLLTGSFPIYDELESLMAQAFGRETAL
                     LFNSGYHANIGILPALADKKTLIVADKLVHASMIDGIRLAQCEFVRFRHHDYVHLEQI
                     LQKNDRTFERIIVVTESVFSMDGDCADLTQLVALKQRYPQVMLYVDEAHAIGVLGEKG
                     LGLAEQQGCINQIDILVGTFGKALGSMGAYVICDQVIRDYLVNKMRPLIFSTALPPFN
                     VAWTHFVFQQLPHLQAERAHLAQLSQHLRQAIVDIFQVPMPSESCIVPYILGDNELTV
                     RTAQRLQQQGYYCLPIRPPTVPKGTSRIRFSLTADMQVAEVEQFIACLRELAE"
     gene            complement(6807..8108)
                     /locus_tag="PXD94_001188"
     CDS             complement(6807..8108)
                     /locus_tag="PXD94_001188"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDL6286062.1"
                     /translation="MNEQQLLECDRQHIWHPYAALNADFPLFAVERAEGVYIQLKNGQ
                     QLIDGMSSWWSAIHGYNHPRLNHAATQQLSQMSHVMFGGLTHQPAVELAQQLVNILPE
                     SLDKIFFADSGSVAVEVAMKMAVQYQHAKGETQRYKFATIRSGYHGDTWHAMSVCDPV
                     TGMHSLFAKGLPVQYFLPQPPVKFGQAWQPEAMQPLQDLLAQHGDEIAGLILEPVVQG
                     AGGMYFYSPEYLVQAKALCEPYGILLIFDEIATGFGRTGKLFACEHANVVPDVMCIGK
                     ALTGGYLTLSATITNQAVADVICSGEAKCFMHGPTFMANPLACAVAAESIKLLLASPW
                     QQRIQQIEQQLRQELRQAEALDCVAEMRVLGAIGVIEMKAPVNMASLPPRFVQNGVWV
                     RPFGKLVYLMPPYIISSDQLSQLIHGTIRTLQEEYAHKESL"
     gene            8250..9137
                     /gene="psd"
                     /locus_tag="PXD94_001189"
     CDS             8250..9137
                     /gene="psd"
                     /locus_tag="PXD94_001189"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755522.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL6286063.1"
                     /translation="MNILEKKAQLSYWQRIKIAFQYVMPQLYLTRLAGWFAKQQWGAV
                     THFVIKLFAKKYHVDMSEAAKPNFSDYASFNEFFIRPLADNARPINQNPTALCLPADG
                     RISQLGHIEQDLLLQAKGHYFSLNDLLAGDEALAHHFKDGEFATTYLSPRDYHRVHMP
                     CDATLCKMIYVPGDLFSVNPFLAEHVPNLFARNERVICVFDTEFGKMVQILVGATITA
                     SMSTVWAGVINPPRPEKITTWAYEGESAVKLLKGQEMGAFQLGSTVINLFEKDRVQLA
                     SHLQVDSPVRMGEILAHQK"
     gene            9162..9716
                     /locus_tag="PXD94_001190"
     CDS             9162..9716
                     /locus_tag="PXD94_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6286064.1"
                     /translation="MSQQFFDQVSLESQLEVSAEAVAKGIFDVLNQNPPALELNEVSH
                     ALQTLQQQAKFKEIEAVGAQFLAENAQKAGVNTTESGLQYEVLVAGEGQIPAREDKVR
                     VHYTGTLIDGTVFDSSVKRGQPAEFPVNGVIAGWIEALSMMPVGSKWRLTIPHNLAYG
                     ERGAGASIPPFSTLVFEVELLAIL"
     gene            complement(9776..10099)
                     /locus_tag="PXD94_001191"
     CDS             complement(9776..10099)
                     /locus_tag="PXD94_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286065.1"
                     /translation="MNWKAVLIFSVLGSLVSCASYSEHAVQRIEAGKSFAVTGNTKRI
                     NTMACQDNDDWYLDGYRVGKSFREHQQKMLIQRTAYCEEQTGKAVPDKFRHSWNSGYQ
                     QGLKR"
     gene            complement(10123..11226)
                     /gene="proB"
                     /locus_tag="PXD94_001192"
     CDS             complement(10123..11226)
                     /gene="proB"
                     /locus_tag="PXD94_001192"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL6286066.1"
                     /translation="MKIDKTLVVKFGTSTLTQGSAKLNLPHMIDIVRQLAQLHQAGFR
                     LVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQIL
                     LTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
                     QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKI
                     SAADVATRSGIETIIAPGNRPNVIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAG
                     IITIDAGAENAMLIQHKSLLPAGIVNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDA
                     LHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL"
     gene            11392..12380
                     /locus_tag="PXD94_001193"
                     /pseudo
     CDS             11392..12380
                     /locus_tag="PXD94_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
     gene            12455..13102
                     /locus_tag="PXD94_001194"
     CDS             12455..13102
                     /locus_tag="PXD94_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL6286067.1"
                     /translation="MTINTYAKFAPTVFVAKCSESHTKGEIITLTSKYGNETEVQVHN
                     LVKQDGEHYFYSFTRCDGMDSQSFAQQKAERYQGYADNAAKRSQDWVNAANEGCDFLS
                     LGEPIKIGHHSEKRHRNLIERNARRMDNAVAEMNKAASYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKTKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            13126..13533
                     /locus_tag="PXD94_001195"
     CDS             13126..13533
                     /locus_tag="PXD94_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL6286068.1"
                     /translation="MNRQGIYINGKQVFDSQGEDIQSIGFFGCNIQTVVGINKGSILV
                     SSNSNQLLVNGQSIELGSEPVLKIEVLGNVQKLSAGAADVSITGDAGSVDTVSGDVSI
                     KGNVASYVKTVSGDVRCRAITGSVETVSGDIYR"
     gene            13631..13981
                     /locus_tag="PXD94_001196"
     CDS             13631..13981
                     /locus_tag="PXD94_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286069.1"
                     /translation="MTTINLSQQERELVEFVWNNRNTPWFFHCSVSNVSRNNTSRQIA
                     VCVIKDNQLISLDELIAKLCGYKLRQNGLYVTVHNMDAVFDTLYRFMCKLNDEMEANE
                     NVCPYLSLAHYEVF"
     gene            14072..14434
                     /locus_tag="PXD94_001197"
     CDS             14072..14434
                     /locus_tag="PXD94_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286070.1"
                     /translation="MIDTFFDLDDTSCYLGNWEDILKEYDDCLSVEDFWHSAKEFDTV
                     PHFGNLYQERVILKLIARFCIELNIEQDSGLVEFDYYINAIDTHFYINSQRICDIDDW
                     NEMLDKIREEMTPAKLVA"
     gene            14547..14807
                     /locus_tag="PXD94_001198"
     CDS             14547..14807
                     /locus_tag="PXD94_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286071.1"
                     /translation="MTFELGRVCATRAVAECFNQHTLVDLLHRYCSGDWGNLCDNDKK
                     ANVKAIERGGRILARYDVEDETLYVITEADRSYTTILFPYEY"
BASE COUNT         4278 a         3201 c         3074 g         4260 t
ORIGIN      
        1 agcattctga aagggaacgc cacttttgtg ggaatgccac ctaaaccctc taccatctca
       61 gtgaagcatc attgagattt cattttactt ccacctcgcg gggaacattt ttcccgtgtt
      121 gggcaaggat gtttctccgc ttttttacat ttggagaaac aaactatgac aactcaaact
      181 tctactcaaa aaaattactt caaccttcat acaacaggca ttggctatct aaacgatatt
      241 cgtttggtag agcctaaaaa aggaaatcct ttttatgcct gccgtatagc agctctggtt
      301 ggcgaatctg actctcctga gtacagatat tttgatatga atgtagtcgg taaagagact
      361 gaaacactta tcaaaagatg ccaaaaagca gttagtgaag agaaaaaagt gcttgtgtca
      421 tttgtaatgg ctgatttgtg gttcgataca tttacttact caacagatag taaattccac
      481 aaaaaaggcg ataccggtgt atctgtaaaa ggtcgcttaa tccgcatcaa aatgattaaa
      541 attgatgggg aaatagccta ccaagaaaag ccaaaatcag atgagcaatc tgagtaattt
      601 tacctaagcc cgacatttta tgttgggctt tattactcga tgtacaagat ttgcgcgtaa
      661 cttttaagac cccagatggc gatgttactg ccgtcaatga tctgactttt tctttaaatg
      721 ccggtgaaac cctcggcatt gtcggcgaat ctggctcagg aaaatcgcaa accgcttttg
      781 ctctaatggg gctattagca gataacgggc gaatttcagg atcagcaaaa tttaatggac
      841 aagaaattct caacttgcct gaaacacaac ttaaccgctt gcgtgctgaa caaattgcga
      901 tgatcttcca agatccaatg acctcgctga acccttatat gaaaatcggc gaacagctaa
      961 tggaagtact gatgttgcat aaaggcatgg atcataaaac tgcgtttgaa gaatcggtac
     1021 ggatgttaga tgcggtgaaa atgccggagt cacgtaaacg tatgaatatg tatccgcatg
     1081 aattttccgg cggtatgcgt cagcgtgtga tgattgcgat ggcattactt tgccgtccga
     1141 aactcctcat tgcggatgaa cccacaactg cgctcgatgt gacggtgcaa gctcaaatca
     1201 tgaccttatt aaacgaatta aaaagcgagt ttaataccgc cattatcatg attactcacg
     1261 atctcggtgt ggtcgcagga atttgcgata atgtgttggt gatgtatgta ggcagaacca
     1321 tggaatacgg caccgctgag caaattttct ataccccaag ccacccttat tctatcggct
     1381 taattggtgc agtgcctcgc gttgacggac aggaagaaga actcattact attccgggta
     1441 atccacctaa cttattacgc ttaccgagtg gctgtccttt ccaaccacgt tgcgttcatg
     1501 caactaaaca gtgcctcaaa gcaccggcgt taaccttgtt aaatgatcaa cgtttacgcg
     1561 cttgttttgt ccctgccgag caattaatcc gaggtaacgc atcatgaaca cagagaaaaa
     1621 agtgatccta gaagtgaagg atttaagcgt tcatttcaac atcaaagata cgaaatcttt
     1681 attctttgct aaacaccaaa ctttaaaggc cgtcaatcat gtctcttttc agttatacga
     1741 aggcgaaacc ttaggtgtgg tcggtgaatc gggctgtgga aaatcaactc tcgcacgcgc
     1801 gattattggc ttagtggaaa gctctggcgg taatatttta tggctaggta aggagttaac
     1861 tcaacaaagt gcgaaagaat ggaaaacgat ccgtagcgat attcaaatga ttttccaaga
     1921 tcctttagcc tccctgaatc cacggatgac cattggcagt atcattgccg aaccattaaa
     1981 aatttaccaa ccccatttaa ccgcggaaga agtgaaagaa aaagtacgcg cgatgatgat
     2041 gaaagtgggc ttattaccta acttaatcaa tcgctatcca catgaatttt cgggtggaca
     2101 atgtcagcgt atcgggattg cgcgcgcctt gattgtggaa ccaaaactca ttatttgcga
     2161 tgagccagtg tccgccttag atgtttcaat ccaagcacaa gtagtgaatt tattgaaatc
     2221 tttacaacgc gaaatggggc tatcattgat tttcattgca cacgaccttg ctgtggtgaa
     2281 acacatttcc gatcgtgttt tggtcatgta tcttggtaac gcggtggaat taggtgacta
     2341 tgaacaagtc tatcacgata ctaagcatcc ttataccaaa gctttgatgt ctgcggtacc
     2401 gattcctgat ccgcaattag aacgcaataa acaaattcaa ttgcttgaag gcgatctccc
     2461 ttccccgatt aatccgccgt cgggctgtgt gttccgcacc cgttgcccac tggcagatca
     2521 acactgtatt gcagacaaac cgctcctcaa aggacgagat aatcacgttg ttgcttgttt
     2581 taaagccgag taatacacac aaccgcacaa aaaagtgcgg ttgttttttt gcgaatattc
     2641 acagtcagac tttcgtgata ctttcgctct gagataagga ttacgataca gagaagttat
     2701 tcaacaatca cacgtgcatt gatcggttgc actggacgat tattgtgtcc tttcatcaag
     2761 gtcgcaaacg cctcaacttg agattggcta atgttgtcat agtgttttaa cactaaccaa
     2821 tttacacctt cagaacaagg tggtgtagtt aatgacccgc tgaaacgata gtaatcgcgt
     2881 tttttcggaa ttaatgttgc tgggtcaaaa gcggtactaa gcgtatattt ctcccctgct
     2941 ttttgcggga agttttgcca aattggggct aaccattggt tttcacttga taatacatag
     3001 agcacgccaa gcacagcaag attcccttca ttgtctgcgt gtacaaaatg tgcttccatc
     3061 gggaaggatt tacctcaaat taagttttca ctcggcacat ggaaatggaa ttgtttaagg
     3121 gtaaaagtct ttccattaag ctgcatgcga ttacttgcct ctttgaaatc cacttgaata
     3181 gtatgcccat tattgacaat ctccacattg gaaggtcgat aatccagctt aatcggcgct
     3241 aacttaccat caatcgtatg cttgatatct attggtgttt ggtttttacc ttcgccacaa
     3301 atacggaatt tgggtgaaag ttcagcccaa tgttctggcg attcgtgtcc ggtgtatccc
     3361 caatgcgtgt gcgtttcttc tttcttaagg gggcttgtac acgctgtcaa tgctaatgca
     3421 cctaacgtaa gcacaaaaag gtttgatttt ttcatagcct agctcctgat taaaagagat
     3481 gaattgatga gacaacctaa actcaatgat gccttaatcc gagacgcttt catcttaggc
     3541 aaaacaacac tccatttcaa gtataacttg ctcaagaata tatcttagac cgaaatcaaa
     3601 tccattattt caaataaggt attaggcgag tatttctcta aataacgacg taaaaaatgt
     3661 tgtgtttctt ggctaatgac ttgatctgtt tccggataca agttgtatat cacagcatgc
     3721 acacttactc ttttggagtt gagtacttgt aaactcaata aggtatgatt aatactacct
     3781 agcttgccag aggtaactaa aatcacgggg tattgatgct gacaaagata atcaagggtg
     3841 gtttcttcct cattataagg tacacacaag ccgcccgccc cttccaataa tacataatca
     3901 tatttttcac acaaaagtgc ggtgcaggtt tcaatttttt tacgttcgat cgtgcgcggt
     3961 tctaatttcg cggctaaatg cggtgagcaa ggataatcaa acacgtaagg acaagtgata
     4021 ccttgcttat cttcttctgt aagctcaata ccttgtattt ttcggtgtat gagtaaatct
     4081 tcggcaattc ctcggcagcc tgtttggatc atcttttgcg tgatgacact tgcgccttgt
     4141 tgcatcagtt tcttggcgta ccaacctgtg gcgatggttt tgccaatatc ggtgtcgatg
     4201 ccagtgataa aaaaaacctt tgccatactt tttccttcta agattaagtt aaacgcgcca
     4261 gcacatacat cggcgtgtaa gtgagttcta ctttgtgttg tgcgttacgg taatgttgtt
     4321 gatattgttg gcaaaaggcg tgcaattgcc cttttgtcca cctttgctgc tgtgtggcag
     4381 taacgcctgt ttgtttcaaa tgttttaaca ccgctaaggg atcatcaaag tgcaacacaa
     4441 tctcctcgtg ttcgagggct aaaatctcaa attcaggcac tagccacttt tgccagtcct
     4501 caagacgtgg ataagttaac cccacaccgg tgaggtgttt gatctgtgtt aaattctcgg
     4561 gcgtgaaagt actgaataac aaaaatccgt gcttttttaa gcgcacttta caacgcgcaa
     4621 taaaacccgc tttatcttca aaccattgca ccacggaagc actggcaatc aaatcatact
     4681 gctgcggaaa gggaagttgt tccgcatcgc cacaatggaa gtgaaaaggt tggggcaaca
     4741 ctgattgcag atgctctcgc atatcgcaaa ggtcattcag ctcccagtgt tcaacctgca
     4801 actgtgacat taattgcttc gttaatccac cagtaccaca tccaatctct aatgcacgtt
     4861 ggaatgcttg tctgtcgata tacgacaata aactaagcaa acgttggtga atctgctgtt
     4921 gtgctctcgc ttgctgatca tagttcgata cagcggcaga aaaacgctgt ttaatgcgtc
     4981 ctttatcaat acgatccatg tgctacaacc aacaagtttc ccaatgggtg aagtgaggaa
     5041 acaacaaatg ctcccctgcg atttctgtta ttgcacaacg atcctgccaa taagcccgct
     5101 gattttgtgc agggaaaatt ttatcttttg agccgacaat cgccttgctc cacgagatta
     5161 aatctgtacg gtgatcttgt tgtaaaaagg tataaagtgc ggtcagttca gcatgaattt
     5221 cctgtaatgt gggacgcatg gaatagtgct gataatctct taacagcgtt tttgaaccac
     5281 aaatacgacg ttcaaattta gtccgattat cttcatttaa accagataac gtgccagcaa
     5341 aaatggcttc gggaatacca tattggtcat cacaaggtaa gcccgtgcca ttgatcgccg
     5401 tagcagatag cagtggataa ccttgcatca cacgctccgc tacccacacg cccattgacc
     5461 atgccaccac gcgaatatgt tgataagcgg aaaaatcaac atcgcaatgc agatctcgat
     5521 aatcatagca aaggcataaa tcataatttt ccggcagaat caggtgctcg accacgctcg
     5581 gcggtgtgcc ccaccctgca aaatagacga tcagattgtc gccttgctgt gaaatatgtt
     5641 gtgttttcat tcagcaagct cccgtaaaca agcaataaat tgttccactt ccgccacttg
     5701 catatctgca gtcagtgaaa aacgaatacg agaggttcct tttgggactg tcggcgggcg
     5761 aatcggtaag caataatagc cttgttgttg caaacgttgc gccgttctta ctgtcagttc
     5821 gttatcgccc aaaatataag gtacgataca actttcactt ggcatcggga cttgaaaaat
     5881 gtcaacaatc gcttggcgta aatgttgact aagctgagca agatgtgcgc gttcggcttg
     5941 caaatgtggt aattgttgaa agacaaaatg cgtccatgcc acattaaacg gcggaagtgc
     6001 cgtggaaaaa atcaatgggc gcattttatt aaccaaataa tctcggatca cttgatcgca
     6061 aatcacataa gcccccatag agcccaacgc cttgccaaac gtaccgacta aaatatcaat
     6121 ttggttgata cagccttgtt gttctgctaa ccctagcccc ttctccccca atacgcctat
     6181 cgcatgggct tcatctacat ataacatcac ttgcggatag cgctgtttta atgcgaccaa
     6241 ctgggtgagg tctgcacagt caccatccat actaaacacg gattccgtga ccacgataat
     6301 ccgctcaaaa gtgcgatcat ttttttgcaa aatttgttca aggtggacgt aatcatgatg
     6361 acgaaaacgc acaaactcac attgtgccaa gcgaatgcca tcaatcatac tggcatgtac
     6421 cagtttatcc gccacaatca gcgttttttt gtccgctaaa gcaggcaaaa tcccaatatt
     6481 agcatgataa ccgctattaa atagcaaggc agtttctcgt ccaaatgcct gcgccattaa
     6541 gctttctagc tcatcataaa tcggaaaaga gcctgttaat aaacgggagg aagaagaagt
     6601 caatgcaggg agttgatctt ggtattgcgt gaaaaatgcc tgacgcaagg cttcgttatt
     6661 ggctaaacct aaataatcat tggaggacat attcagcatc gtattgtcct cacgttgaat
     6721 aaaacgccct cggtgaatta gttggggcag ttggcgaaat tgattctgcg cctttaaatc
     6781 agctaactgc tgctgataat acctgatcat agagattcct tatgtgcata ttcttcttga
     6841 agggtgcgaa ttgtgccatg aattaattga gacaattgat ccgatgagat gatatacggt
     6901 ggcatcaaat acactaattt gccaaatgga cgcacccaaa cgccattttg cacaaagcgt
     6961 ggtggtaaac ttgccatatt gacaggcgcc ttcatttcaa tcaccccaat cgctccaagt
     7021 actctcattt cagcgacaca atctaaggct tccgcttggc gcaattcttg acgtaattgc
     7081 tgttcaatct gttgaatacg ttgttgccaa gggcttgcca ataacaattt gatactttct
     7141 gctgctaccg cacacgccag tggatttgcc ataaacgtcg gaccgtgcat aaaacatttt
     7201 gcctcgccag aacaaatcac gtctgctacc gcttggttgg taatggtagc ggatagagtt
     7261 aaataccccc ccgttaaagc tttaccgata cacatcacat ccggcacgac attggcatgt
     7321 tcacaagcaa ataatttgcc tgtacgccca aatcctgtgg caatttcatc aaaaatcagc
     7381 aaaatgccgt acggttcgca taaggctttt gcttgcacca aatactcagg ggaataaaaa
     7441 tacataccac ccgccccttg cactaccggt tctaaaatta aacccgcaat ctcatcacca
     7501 tgttgtgcca ataaatcctg taacggttgc atcgcttcgg gttgccaagc ttgcccaaat
     7561 tttaccgggg gttgcggtaa aaaatattgc acgggtagcc ctttggcaaa caagctgtgc
     7621 atgccggtca cgggatcaca cacggacatc gcatgccaag tatcaccatg atagcccgaa
     7681 cgaatcgtag caaatttata gcgctgtgtt tcgcctttag catgttgata ttgcactgcc
     7741 attttcatcg ccacttccac ggcgaccgaa ccactatcgg caaagaaaat tttatccaaa
     7801 ctttccggca agatgttgac cagttgctgc gccagttcta ccgctggttg gtgcgttaat
     7861 ccaccaaaca tcacatgact catttgactc agttgttgcg tggcagcgtg gtttaaacgg
     7921 gggtgattat agccatgaat cgctgaccac cacgatgaca tgccatcaat caactgctga
     7981 ccgtttttta actggatata cacaccttcg gcacgttcta ctgcaaataa gggaaaatcc
     8041 gcatttaacg ccgcataagg gtgccaaata tgttgtcgat cacattctaa aagttgctgc
     8101 tcattcatcg tttcacctcg gttgtgaatc gccctttcaa ctggcgtaaa ttcacgcaaa
     8161 tacttacata aacggaataa cttgttataa tcgccaagca tttggctttt caagcccatt
     8221 attctaactt aaaaagatga tttattacta tgaatatatt agaaaaaaaa gcacaactga
     8281 gttactggca acggattaaa attgcctttc aatatgtgat gccacaactt tatttaacac
     8341 gtcttgcagg ctggtttgca aaacagcaat ggggtgctgt cacacatttt gtgattaaat
     8401 tattcgcgaa aaaataccat gtggatatga gtgaagctgc aaaaccaaat ttcagcgact
     8461 atgcgagctt taatgaattt tttatccgtc cattagcgga caatgcccgt ccaattaatc
     8521 aaaatcctac cgcactttgt ttaccggcag atggtcgtat tagtcagtta ggacacattg
     8581 agcaggattt attgctacaa gccaaaggac attatttcag cttgaacgat ttgttagctg
     8641 gcgacgaggc gctcgctcat cattttaaag atggcgaatt tgccaccacc tacctctcac
     8701 cacgcgatta ccaccgtgtg catatgcctt gtgatgccac cttatgcaaa atgatttatg
     8761 tgccaggtga cttattctca gtcaatccat ttttagccga acacgtgcca aacttatttg
     8821 cacgtaatga acgcgtgatc tgcgtattcg ataccgaatt tggtaaaatg gtccaaattt
     8881 tagtcggcgc aaccattacc gcgagtatga gcaccgtgtg ggcaggtgtg atcaacccgc
     8941 ctcgtccaga aaaaatcacc acctgggcct atgaaggaga aagtgcggtc aaattactga
     9001 aaggacaaga aatgggagcc tttcaattgg gctcgacggt gatcaattta tttgaaaaag
     9061 atcgtgtgca actggcatct catttacaag tggatagccc ggtacgtatg ggtgaaattc
     9121 ttgcccacca aaaataattt ttaaccacaa taggaaaaac aatgagtcaa caatttttcg
     9181 accaagtgtc attagaaagc caacttgaag tgagcgccga agccgtcgcg aaagggatct
     9241 ttgatgtgtt aaatcaaaat ccaccagcac ttgagttaaa tgaagtgagt catgccctac
     9301 agactttgca acaacaagcg aaattcaaag aaattgaagc cgttggtgca caattcttag
     9361 ctgaaaacgc gcaaaaagcg ggggtaaata ctacggaatc tggtttacag tatgaagtgt
     9421 tagtggctgg tgaagggcaa attccggcac gcgaagacaa agtgcgtgtg cattacactg
     9481 ggacattaat cgacggtacc gtctttgaca gctcagtaaa acgtggtcaa cctgcagaat
     9541 tcccagtaaa tggcgtgatc gctggttgga ttgaagcctt gtccatgatg ccagtgggtt
     9601 caaaatggcg tttaaccatt cctcacaacc tcgcttatgg cgaacgtggt gctggtgcct
     9661 ccattccgcc attcagtacc ttagtgtttg aagtcgaatt attagccatt ctttaaagct
     9721 aaacgctaga cataaaaaag tgcggtcatt ttccgcactt ttttatgtct agcgtttatc
     9781 gtttcaagcc ttgctgatag ccgctgttcc acgaatggcg gaacttgtcc ggcacagcct
     9841 tgcccgtttg ttcttcacag taagccgttc gttgaatcaa cattttttgt tggtgttctc
     9901 gaaatgattt tcccacacga tagccgtcca aataccaatc atcattatct tgacatgcca
     9961 tggtattaat ccgttttgta ttccccgtca ctgcaaaaga tttgcctgcc tcaatacgtt
    10021 gcaccgcatg ttcagagtat gaagcacagc tcaccagcga acctagtaca ctgaatatca
    10081 atactgcttt ccaattcatc ttgttacctt tctttttctg atttataaca caatcatatc
    10141 gtctcggtgt accgcaaccg ccccatattc ataaccgaga acctgttcga tatcttgcga
    10201 atgtttgcct tgaattaaat ggagcgcatc gctgttataa cgtggcatcc ctaaggcaac
    10261 atccttgcct tgctgagtac gaatttttac cacttcaccg cgtgaaaagc gcccttccac
    10321 gtttacaatc ccagcaggca ataacgattt atgttgtatt aacatcgcgt tttccgcccc
    10381 cgcatcaatg gtaataatac cggctgacgg cgccgcatat agccattgtt tacgactttc
    10441 taagcgatct gcttgcacac taaatttggt gccaatggct tgcccataag ctaagtccac
    10501 aatcacattc gggcgattac ccggtgcaat aatggtttca atgccggagc gggttgccac
    10561 atccgcggca ctgattttgg tggacatgcc acctgtgcct aacgaggtgc cacttccgcc
    10621 cgcaatcgcg cgaatatgat cggtaatttt gtccacgtcg gcgatcaatt tggcgtctgg
    10681 atgtttgcgc ggatcacgat caaataaacc ttgctgatca gtaagcaaat acaactgttc
    10741 tgcttgcacc aaaattgcca ctaacgcgga taaattatcg ttatcgccta ctttaatttc
    10801 tgacgtagcg acagcgtcat tttcattaat cactggaata atctgattgt ctaataaggc
    10861 gcgcaaagta tctctggcat ttaaaaaacg ttcacgatct tcaatgtcgg cacgggtcaa
    10921 taaaatctgc ccaatatgaa tatcataaat ggcaaacaat ttttcccacg tttgaatgag
    10981 ctgactttgt cctaccgcag ccaataattg tttcgaggca atcgtcggtg gcaattgcgg
    11041 gtgatttaaa taatgacgac ctgccgcaat cgcccctgag gtaacaatca ctaaacgaaa
    11101 gcccgcttga tgaagctgtg cgagctgacg cacaatatct atcatatgag gtaaatttaa
    11161 cttcgccgat ccttgcgtaa gcgtactggt tccaaatttt acaacgagtg ttttgtcgat
    11221 tttcatcttc tctatcttct tatttttgtc gcacaaaatt gaagctaact taccattaat
    11281 catcagaaaa atcatctcgt ttacttgcta ttttctatga tttagcacaa attttttctc
    11341 actaaaaggc atataataaa gcttcttgat ttttcagcta ggaacccgat tatgacctca
    11401 acctcttcac aagaaacgga gcggcaacga gaaatcactc gcctttgtgt tcaagttgcc
    11461 caactcttat acaacatggt gcagaaagtg ccatcgttgt gcaaatggca catcgcttag
    11521 gcttagccct cggtgtagat agcgtagaat gtgccctcac gccaaatgcc attattctta
    11581 ctacattagc ccaagaccat tgcatcacga cagtccgtaa aaaccaagac aaaggaatta
    11641 atatgcaagt ggttaccgaa gtgcaacgta ttgtgattac agcagaacgt aaaatctatg
    11701 atatgcaaca agtgcatgcc aaactggctc gcattaaacc gcttaaatac aatcgctatt
    11761 ttgtgatgtt gatgattggc gtatcctgtg ccagttttgc tcatttatct ggtggggacg
    11821 cactagtttc attggttaca tttctcgccg caacactcgc catgtatgtt cgccaatcac
    11881 ttgccgcaag acactataac cccttaattg tatttgcggt gaccgccttt gtcgcctcac
    11941 taattgcggg tatcgcgttg aaatataaca tcggcaatca gccccaaatt gccttggcat
    12001 ccagcgtatt attgctcgtt cccggctttc cactgattaa tgcactcgcc gatattttaa
    12061 aagggcatat caatatgggg attgcccgtt gggcactcgc gacaatcctc acatttgggg
    12121 catgcttagg cattgtattt gcgctgagtt tattcttaga ggaaatctgt atgctaaata
    12181 aattcattca agatgaattt ctacggctag atcaacgtta tgcagcttgg tatcaaagtg
    12241 aagaaacgga tgaagttgcc agaatgggga ttcaagaaag tgagcggatt ttgaaaattc
    12301 tgtatgcact gaaatataac gaagacccta tgcaaaatgg tttaggctat attgcattta
    12361 tggcatcaaa aggccaataa tttacttatc tcaacaattt acccaccggg gaaactcctc
    12421 gatggcgttt cccctacttt ataggagaaa cgctatgact attaacactt atgcaaaatt
    12481 tgcacctacc gtttttgttg caaaatgctc tgaatcacat accaaaggtg aaattattac
    12541 gctaaccagc aaatatggta atgaaaccga ggttcaagta cataatcttg ttaaacaaga
    12601 tggcgagcat tatttttatt catttactcg ttgtgatggg atggatagtc agtcatttgc
    12661 tcaacaaaaa gcagaacgct atcaaggcta tgcagataat gcagccaaac gtagtcaaga
    12721 ttgggtaaat gccgctaatg aggggtgtga ttttttgagt ttaggtgagc caataaaaat
    12781 tggtcatcac agcgaaaaga gacaccgtaa ccttattgaa cgtaatgctc gccgtatgga
    12841 caatgcggtt gcagaaatga acaaagctgc aagttatgaa tctcgtatcg catattggga
    12901 aaaaatggca ggaaaaattg acctgtctat gccggaaagt ctggaatact ttaagtttga
    12961 attggataaa gccaaaacaa agcacaaaga attgaaggac aaccccgaga aacgagagca
    13021 tggttattca ctgacttatg ctaaaaaagc agttaatgaa cttgagaaaa aagtaaaatt
    13081 agctgaatta ctttgggcat aattatttac aaaggagatg acgatatgaa tcgacaaggt
    13141 atttatatta atggcaaaca ggtgtttgat tcacaaggcg aagatattca aagcattgga
    13201 ttttttggct gcaatattca aacggtcgta ggcataaaca aaggaagtat tttagtctcg
    13261 tccaatagta atcaattatt ggtgaatgga cagtcgattg aattaggttc tgagccagtc
    13321 ctcaaaattg aggttttggg caatgtgcag aaactgagtg ccggtgccgc agatgtctct
    13381 attacaggag atgccggcag tgttgacaca gttagcggtg atgtctctat taagggaaat
    13441 gttgcctctt atgtgaaaac cgtgagtggt gatgttcgct gtcgtgctat taccggttcg
    13501 gtggaaaccg tctccggtga tatttatcgc tgatatttac tcatcttatt atttaccacc
    13561 ctattgggaa gcaattttcc caaggtgggc agattgtttc ccatttttat tttgaatttg
    13621 gagaaacatg atgaccacta ttaacctttc acaacaagaa cgtgagttag ttgaatttgt
    13681 ctggaataac cgtaatacac cttggttttt tcactgtagt gtatcgaatg tgtcgagaaa
    13741 taatacctct cggcaaattg ctgtatgtgt cattaaggat aatcaattga ttagccttga
    13801 tgaattgata gcaaaattat gtggttataa actcagacaa aacggattat acgttactgt
    13861 tcataatatg gatgctgttt ttgatacctt atatcgcttt atgtgcaagc taaatgatga
    13921 gatggaggca aatgaaaatg tttgccctta tttatcatta gctcactatg aagtgttttg
    13981 attggtatca gaactatcca ccctgtcggg aaacaatttt tccgataggg cagattgttt
    14041 cccactttta ttttgaattt ggagaaacat aatgatagac accttttttg accttgacga
    14101 cacctcatgc tatttaggta actgggagga tatactgaaa gaatatgatg attgtctttc
    14161 agttgaagat ttttggcatt ccgctaaaga atttgatacc gtacctcatt ttggcaacct
    14221 ataccaggag cgtgtcatcc ttaagttgat tgctcgattc tgtattgagc tgaatattga
    14281 gcaagattca ggtttagtcg aatttgatta ttatatcaat gcaatagata cgcatttcta
    14341 tattaatagc caaagaatct gtgatataga tgattggaat gaaatgttag ataagattcg
    14401 tgaagaaatg acgccggcta aattagttgc ctaatttttg caaattttta ctaaaaatga
    14461 cgcttactgt cgtcttaatt cacccacttg gggaaatatc tccccaatgg gatgacattt
    14521 cccctctttc tgtttaggag aaatgtatga cttttgagct tggtagagta tgtgcaactc
    14581 gtgcagttgc tgaatgtttc aatcaacata cattagttga tctacttcat cgctactgca
    14641 gtggtgattg gggaaatttg tgtgataacg acaagaaagc caatgtgaaa gctattgaac
    14701 gcggaggacg cattttagct cgctatgatg tggaagatga aacactttat gtaatcacag
    14761 aggctgatcg tagttacact acaattttat ttccttatga atattaatgg agc
//
LOCUS       DANLXQ010000052        14803 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.052, whole genome shotgun sequence.
ACCESSION   DANLXQ010000052 DANLXQ010000000
VERSION     DANLXQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14803
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.052"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..461)
                     /gene="sixA"
                     /locus_tag="PXD94_001199"
     CDS             complement(<1..461)
                     /gene="sixA"
                     /locus_tag="PXD94_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL6286072.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKA"
     gene            588..2027
                     /gene="pyk"
                     /locus_tag="PXD94_001200"
     CDS             588..2027
                     /gene="pyk"
                     /locus_tag="PXD94_001200"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL6286073.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGIP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEGVKVHTEVTVGGPLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            2123..3160
                     /locus_tag="PXD94_001201"
     CDS             2123..3160
                     /locus_tag="PXD94_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6286074.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGIMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            3200..4258
                     /locus_tag="PXD94_001202"
     CDS             3200..4258
                     /locus_tag="PXD94_001202"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL6286075.1"
                     /translation="MNTVFNQDSVHNVNIQDEKVLLTPQELKQEFPLPEHLRNQIERS
                     RKTISDIIHRRDKRQLIVIGPCSIHDPVAALEYGKKLKALSEQVSDKLYIVMRVYFEK
                     PRTTVGWKGLINDPKMDGSFDVETGLRIARKLCLDLAELGLPLATEALDPMTPQYLAD
                     LFSWSAIGARTTESQTHRELASGLSMAVGFKNGTDGDLAVALNAMQSSAQSHSFIGIN
                     QQGQVTLLKTKGNADGHIILRGGKTPNYEKQYVDDCEWVLKQANLPTAIMIDCSHGNS
                     NKDYRRQPLVAENVLEQLLAGNTSIIGLMIESNLHAGNQSSELPFEQMKYGVSITDAC
                     IDWQTTEELLTHFAEKLR"
     gene            4313..5434
                     /gene="tyrA"
                     /locus_tag="PXD94_001203"
     CDS             4313..5434
                     /gene="tyrA"
                     /locus_tag="PXD94_001203"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL6286076.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(5625..6710)
                     /gene="recF"
                     /locus_tag="PXD94_001204"
     CDS             complement(5625..6710)
                     /gene="recF"
                     /locus_tag="PXD94_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL6286077.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            6850..7179
                     /locus_tag="PXD94_001205"
     CDS             6850..7179
                     /locus_tag="PXD94_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL6286078.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDDKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            7187..7792
                     /gene="recR"
                     /locus_tag="PXD94_001206"
     CDS             7187..7792
                     /gene="recR"
                     /locus_tag="PXD94_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL6286079.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
     gene            7864..8190
                     /gene="grxD"
                     /locus_tag="PXD94_001207"
     CDS             7864..8190
                     /gene="grxD"
                     /locus_tag="PXD94_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HDL6286080.1"
                     /translation="METIDKIKQQISENPILLYMKGSPKFPSCGFSARAVEAVINCQV
                     PFGYVDILTNPDIRAELPKFANWPTFPQLWVEGELVGGCDIVLEMLQKGELQTLLKET
                     AAKHAA"
     gene            8332..9369
                     /locus_tag="PXD94_001208"
     CDS             8332..9369
                     /locus_tag="PXD94_001208"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL6286081.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            9396..9944
                     /locus_tag="PXD94_001209"
     CDS             9396..9944
                     /locus_tag="PXD94_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDL6286082.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCSSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            9953..10297
                     /locus_tag="PXD94_001210"
     CDS             9953..10297
                     /locus_tag="PXD94_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL6286083.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(10330..12108)
                     /locus_tag="PXD94_001211"
     CDS             complement(10330..12108)
                     /locus_tag="PXD94_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL6286084.1"
                     /translation="MSTLDFHFIHRFHQQVQALSHHPALRFMQDEKWSEISWLELQKQ
                     VNNLSYALLANGLAVQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQVRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(12334..12903)
                     /locus_tag="PXD94_001212"
     CDS             complement(12334..12903)
                     /locus_tag="PXD94_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL6286085.1"
                     /translation="MNQENHKIEDIIRIFNQCFSDEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            complement(12913..14676)
                     /locus_tag="PXD94_001213"
     CDS             complement(12913..14676)
                     /locus_tag="PXD94_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL6286086.1"
                     /translation="MIRYFFSLVLSLLMINSSHANLFSQPKFLKSEEAFVFSIQNENE
                     TLQLNWDIADNYYLYKKEIRVTPQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKV
                     PFKEIKDNASIVVEYQGCTKGFCYPPERVELKLDSILSATPEELAQLEAQAVEKSENF
                     AKTDNMAPPENVAKAEQDQLAENLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIV
                     IGNKQRPSTAKALLLSIAYVQGMAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVF
                     ILLACSMFGLYEIQLPNSWQQKLNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLS
                     GALLYVAQSGDLVTGGLALYLLALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFV
                     MLALPVFLLSRVLPSHYEPFLWSALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIA
                     AKPWADLAWNGSTTQQQTTPHLALEKIQSLADLETKLTASKGKKVMLDLYADWCVACK
                     EFEKYTFSDQNVQNKLNEMVVLQIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEM
                     SQSRVTGFLDAEQFLAWLNKL"
BASE COUNT         4453 a         2854 c         3203 g         4293 t
ORIGIN      
        1 gcttttgtct ttacccaatt aatttctgct atggttgaag ttggaaattg tacattgctt
       61 tggttattcg ttaaaatttg gacgagatcg aacacaagtg ggagatgtga aatcagaaga
      121 atattttttg cgccttcctc tcttaaaaaa tgtagatagc tttctactgt atgcgggttt
      181 ccgtcaggcg taacctcgtc ccaactttca atgaggttgg taaaatgttg gggaaaattg
      241 accgcttgca taccaataag cacattttca agcgtttgtt gcgccctaag gtatgggctg
      301 acgagaattt tatctaattg aatttgttgg ttggctaaat gttctcctaa ccattttccc
      361 tgtgaaatgg tgttttttat accattgtcg gttaaacagc gagcagcatc actcgaagca
      421 ttaaagcctg cttccccatg gcgcataatc caaatattca taacattctc ctttagttgt
      481 atttaaactc tcttgattta ggcgtataat gaccctcgtt taacgcataa tcaagatgct
      541 tagaaagatt ttaacttaat ttttaaattt attgatggag tcaaataatg tctagaaaac
      601 tcagaagaac gaagatcgtt tgtacaatgg gacctgcaac agatcgtggc aatacgttgg
      661 aaaaaatcat tgctgccggt gcaaatatgg ttcgtatgaa tttctctcac ggtattcctg
      721 aagatcacat cgaacgtgcg aataaagtgc gtgaaattgc tgcaagatta ggtcgccacg
      781 ttgcgatttt aggcgatttg caaggtccta aaattcgtgt ttcgacattt aaagacggta
      841 aaattttctt aaatattggt gataaattca ccttagacgc agatttaccg cgtggagaag
      901 gccatcaaga agctgtaggt ttagattaca aaaacttacc aaatgatgta gtacctggtg
      961 atattctatt gttagatgac ggtaatgttc aactgaaagt gttggaagtg gaaggcgtta
     1021 aagttcatac tgaagtgaca gttggtggcc cgttatcaaa caataaaggg attaacaaat
     1081 taggtggtgg tttatcagct cctgcattaa ccgacaaaga taaagaagac attaaattag
     1141 cggcgaaaat cggtgtggat tacctagcgg tttcattccc tcaatcaagt gcagatttac
     1201 attatgcacg tgaattagca aaagaagcag gcttagaagc gaaaattgta gcgaaggttg
     1261 aacgtgccga aactgttgcg actgaagaag caatggacga catcatttta ggtgcggacg
     1321 ttgtgatggt tgctcgtggt gacttagggg ttgaaattgg tgatgcggct ttagtgggtg
     1381 tgcaaaaacg tttaatccgc cgtgctcgta agctaaatcg tgtggtaatt accgcaaccc
     1441 aaatgatgga atcgatgatt aaaaaaccaa tgccgacccg tgctgaagtg atggatgttg
     1501 caaatgctgt gttagatggt actgatgcgg taatgctttc cggcgaaact gcaaacggtg
     1561 actaccctgt tgaaacggta aaatcaatgg cagaagtgtg cttaggtgct gaaacaatgc
     1621 caagcattaa catttctcgc caccgtatgg aaggtacgtt taaatctatc gaagaagctg
     1681 tggcaatgtc tgcaatgtac accgcaaacc atttagaagg tgtatcggca attatttcat
     1741 taactcattc aggcgaaacg gctaaattaa ccagccgtat cagctcaggt ttaccgattt
     1801 atgcgatgtc tcgtaaccaa aaagcattaa acctttgtgc gttatatcgt ggtgtaacac
     1861 cggtatatta caatgaagac agccgcacaa tggaaggggc gaaaaaagcc attgctcaat
     1921 taaaagaaca aggctattta ttaaccggcg aattagtgct tttaactcac ggagatgaat
     1981 taaaagctgg cggtacaaat acttgtcgta tcttaagagt tgaataatct aaacgattaa
     2041 cgcaaaagcc acataaaaca ctttatgtgg ctttttttct gtcttaatat agcttaatgt
     2101 aatctgaact attgatatta ttatgcacaa taaacttctt tctatctgta ttctccgcct
     2161 ttctgccatt ggcgacgttt gccatacctt agcggttatt caagctattc aacgaaaata
     2221 tcctagtgct gaaattactt ggattatcgg caaaacggaa gcgtatttaa tgaaagatat
     2281 tccgaatgtt cacttaattc cttatgataa aaaaataggg tggaaaggta tctttacact
     2341 ttggcaaaaa ctttctcaca agcggtttga ttttttatta aatttacaaa cggcattccg
     2401 agcttcaatt ctatcgcttg ggattaaagc aaagaaaaaa atcggcttta atcaagacag
     2461 agcaagggaa atgcaatggt tgtttaccaa ccagaaagta gaaatgacag actctcctca
     2521 cgtgcttgac gggcaaatga tgtttgccaa agccattggg ataatggatt tatcggcaca
     2581 atggaattta cctgtgagtg atgaagattt agcttatggc aaacaattta ttgattttac
     2641 aaggaaaaat atactcattg ctccttgttc aagcaagaaa gagaaagatt ggttgccgga
     2701 acgctatgca gaaattgcta actggctaag taagcaaaat atcaatgtat taattgcagg
     2761 ttctccatcg caatatgaaa tggaaaccgc agccaaaatt caacaacttg ccccgaattg
     2821 tacgagtatt gccggtaaaa ccacgctaaa acagcttgca ggtgttattc gtcaggttga
     2881 tttggtgatc tcttcagatt caggtcctgc ccatatcgcc actacgcaaa atacgccggt
     2941 gattgggttg tatgcgattc ataatccaag acggacaggc ccttacaatg acttagacaa
     3001 agtggtttcg gtatatgatg aagcgatttt gcaatcttat ggtaaacctt ggcagcaatt
     3061 agcttgggca actaaagcta aaggcaaaaa ttggatggaa aaaatcacgg tggagagtgt
     3121 gaaacaaaaa gtagttgaaa ctttaaaaat tacgttgtaa agtttgtgta ttaaaagatt
     3181 tacacaaaag gaaattaaaa tgaacacagt gtttaatcaa gatagcgtgc ataacgtaaa
     3241 tatccaagat gaaaaggtat tactgacccc acaagagtta aaacaagaat ttccgttgcc
     3301 ggaacattta cgcaatcaaa ttgaacgttc acgcaaaacg atttccgata ttattcacag
     3361 acgagataaa cgccaactga ttgtgattgg accttgttcg attcacgacc cagttgctgc
     3421 ccttgaatat ggtaaaaaat taaaagctct ttcggagcaa gtgagtgata agttgtatat
     3481 tgtgatgcgg gtttattttg aaaagccccg cactactgta ggttggaaag ggctgattaa
     3541 cgatccgaaa atggacggct cttttgatgt cgaaaccggc ttacgtatcg cacgaaaact
     3601 ctgtttggat ttagccgaac ttggcttgcc gttagcgacc gaagccttag atccgatgac
     3661 accacaatat ttagcggatt tgttcagctg gtcggcaatt ggagcaagaa caactgagtc
     3721 tcaaactcac cgtgaattag cctcaggttt gtcaatggcg gtaggcttta aaaatggtac
     3781 agatggtgat ttagccgttg cacttaatgc gatgcaatct tccgctcaaa gccatagctt
     3841 tatcggcatt aaccaacagg ggcaagttac tttactcaaa acaaaaggta atgcagacgg
     3901 acatattatt ttacgtggcg gtaaaacccc aaattatgaa aagcaatatg ttgatgattg
     3961 tgaatgggtg ctaaaacaag caaacttacc tacagcgatt atgattgatt gcagccacgg
     4021 caattccaat aaagattacc gccgccaacc gctggtggca gaaaatgtat tagagcagct
     4081 acttgctggc aatacttcga ttatcggttt aatgattgaa agcaatttac acgcaggtaa
     4141 tcaatcatca gaattgccgt tcgagcagat gaaatacggt gtttctatta cggatgcttg
     4201 tattgactgg caaactacag aagagctttt aactcacttt gctgaaaagt tgcgttaaag
     4261 ataatttgca aaaaattgca taaaaacgac cgcttgtaaa ttggtaacaa atatgaaccc
     4321 attagcccca ttacgagaac aaattgatca agttgatcaa caacttatcg ccttactttc
     4381 tgaacgctta gctcttgtgg cagaagttgg gaaagtcaaa tcagaacacg gcattcctgt
     4441 ttatgctccg gagcgagaaa ccgcaatgat ttccgcccgc cgctctgaag cggaaaaaca
     4501 aggtattccg gcggatctca ttgaagatgt gctacgccgt ataatgcgtg aatcttatgc
     4561 caatgaaaac aaacacggct tcaaaagagt taattcggac attcaaaaaa tcgtgattgt
     4621 tggtggtaaa gggaaattag gtgggctgtt tggcaattat ttaaccctat ccggctataa
     4681 tgttgaggca ttaggacgtg atgattggga caatgccgag cagattttag ccaatgcaga
     4741 tgtggttatt gtgtgtgtgc cgattgccaa caccctagca accatcgagc gtttacaatc
     4801 ttatttaacc gaaaatatga ttttggcgga tttaacctcc gtcaaacatc agccgttgca
     4861 aaaaatgctg gaagtgcata atggagcggt agtcggttta cacccgatgt tcggaccgga
     4921 tattgcaagt caggcaaagc aagtggtcgc ttgctgccac gggcgtttcc ctgaacgtta
     4981 tcaatggttg cttgagcaaa tcaaaatttg gggggcaaaa attgaagaga ttgaggcagc
     5041 agcacacgat aatgcaatga cctacattca agccctacgc cacttttcca cttttgcatt
     5101 cgggctacat ctttctcgcc agccggtgca actctctcaa ttattggcac tatcttcgcc
     5161 gatttaccgc ttagagttgg caatgattgg gcgtttattt gcccaagacg gcagcttata
     5221 tgccgatatt attgccgata aaccggaaaa tttagccacc atcgaaacct taaaagagag
     5281 ttttgaacaa gggctagatt tctttaaacg caacgacaaa gccggtttta tcaaggcttt
     5341 tgaggaagtg catcactggt ttggcgatta ttccgagcag ttcttgaaag agagtagagt
     5401 attgctccaa caggctcacg attcaagaaa gtaaaggtag gttgaggttt tgctctgata
     5461 gcactagcgt ccacgctagt gctatttaat attctacaag cggttaataa agagctgttt
     5521 tttgcaaatt tttatgcaaa attatccgct tgttttggca cgagcgtgga cgctcgcgcc
     5581 atcggaagaa acatttttgt aaaattgccc ccaaatctga ccgcttactg tggctgaatt
     5641 tctccatttt ttaccgtaaa caaacaatca tcgcttctgt cttgccattg catttgattg
     5701 agctgatctt gagtaatggc ggtaacgaat acttgtgagc cactttctct taaacgatgt
     5761 gctaaaagct cacgtttggt tgggtcgagt tcggaggcaa aatcgtcaat taaaaatagg
     5821 cattggcgtt gtttttgggc gaccaaatgt tcgccttgag ccagtcttaa cgcacacatc
     5881 aatagcttta attgcccacg ggaaagcaca tcttccaccg gtaagccgtt cgctttaaaa
     5941 cggaaatcgg ctttttgagc tccaatcatc gtatagccaa gagctttatc ccgctcaaaa
     6001 ccttgtgcca gaatctcggc ataatccgcc cctttttccc agccttgatg aaagctcaag
     6061 ccgatttcga tttccggcag gaaaaattgg caggttttct caatttcggg gcgtaacgct
     6121 tcggcgtatt cggctcgcat ttcgctcacg gcataagtgg ttttcaccaa ttcgatatcc
     6181 cacgctttga gttcttgata agagcggact tgttgcaaag ccgaattacg ttgctttaat
     6241 aaacggcgaa gattgttcca atgagcgtag aaatcagggt gctggtgaaa taagccccag
     6301 tccaaaaaag cacggcgaaa gctcggacca ccgttaagta aggttaagcc ttcgggggtg
     6361 atcacttgca tcggcaataa atgggctaaa tcggcaattt tgttgccgtc ttcaccgttg
     6421 attttaagtg tcgtctcgcc actgcgaaat ttctgaatgc cgaccgacca actgtgcttt
     6481 gcctcgtcaa ttttaccgtg tagtacgaaa tcgtctttat cgtaatggat aatgcggttg
     6541 ctgatatggc ttttgaatga acgcccgtgt ccaagataga aaattgattc caacaaactg
     6601 gttttgccac ttccgttcgc cccgactaaa aaattaaaat gcgggctaaa tgctaaatcg
     6661 gtatgttgga tatttctaaa atggttgatt aataaacggg ttaaagccat agttaaagtg
     6721 cgaggaataa aaagagtagc cattttagca aatttgcaaa attttgggaa aaattaaccg
     6781 cttgcggatt agaaagtggt tcagtagaat aagccgttta ttttttattg aaaattaaga
     6841 ggaaaaatta tgtttggaaa aggtggctta ggcggcttaa tgaaacaggc tcagcaaatg
     6901 caagagcgta tgcaaaaaat gcaagaagag atcgcccaat tagaagtaac aggcgagtct
     6961 ggtgcgggtt tggtaaaagt cactattaac ggggctcaca attgccgccg aattgaaatt
     7021 gatccatcat taatggaaga tgataaagag atggtggaag atttagttgc cgctgccttt
     7081 aacgatgcgg ttcgccgtgc agaagaaatg caaaaagaga aaatggcaag tgtgactgcc
     7141 ggtatgcaac tgccgccggg tatgaaattc ccattctaat ttaagtatgc aaaccagtcc
     7201 tttacttgaa aatttgatgg aagccttgcg tgcattgccc ggtgtcggac cgaaatcggc
     7261 acagcgaatg gcttaccatc tgttgcaacg taatcgttcc ggcggtgtgg cgttgtcaaa
     7321 agccttaaat gaggcaatga gccatatcgg acattgcaaa tcgtgccgta cttttaccga
     7381 agaggaagag tgtacgattt gtaaaaatcc tcgccgtcag atgagcgggc aactttgtgt
     7441 ggtagaaatg ccggaagaca ttcaagcgat tgagcaaacc gggcaatttt cagggcgtta
     7501 ttttgtgctg atggggcatt tgtcgccgat tgatggtata ggccctcgtg aaatcggctt
     7561 agatttattg caacaacgct tggaacagga atctttccac gaagtgattt tggcgactaa
     7621 tccgaccatt gaaggtgatg ctaccgcaaa ctacattgct gaaatgtgca gaatgtataa
     7681 cgtgaaagct actcgtattg ctcacggtgt gccggtcggt ggttcattag aaatggttga
     7741 tggcacaacg ctttctcact cttttgcagg acggcgtgat attgatattt aaagctattt
     7801 ctgtgctaac ttaattctga tataattact caactatttt aaattattat tttgaggcta
     7861 aaaatggaaa ctattgacaa aatcaaacaa caaatcagcg aaaatccgat tctactttat
     7921 atgaaaggtt caccgaaatt cccatcttgt ggtttctccg ctcgtgcggt ggaagcggta
     7981 attaactgcc aagtgccttt cggttatgtg gatattttaa ccaatccgga tattcgtgcg
     8041 gaattgccaa aatttgcaaa ctggccgact ttcccacaat tatgggtaga aggtgaatta
     8101 gttggcggtt gtgacatcgt attagaaatg ctccaaaaag gcgaacttca aaccttatta
     8161 aaagaaaccg cagcaaaaca cgcagcataa tttcaaacca gtatgttaag gctcttatgt
     8221 ttaagagcct taattttttc ttgcgattta ggcttaaact tcttacctca agagctattt
     8281 tgttttagaa tgtcaaaatt atttcgtaac ataaaaataa aaggacacaa catggcatta
     8341 aaagagttag attgggcaaa tttaggcttt tcttatatca aaaccgatta ccgtttcatt
     8401 gcacattgga aagacggaaa atggaatgaa ggcgaattaa ctcaagacaa tacactgcat
     8461 attcacgaag gctcaactgc cctgcactat gggcaacaat gttttgaggg gttaaaagcc
     8521 tatcgctgta aagatggctc aattaactta ttccgcccgg atcaaaatgc cgagcgtatg
     8581 caacgcaccg cagatcgttt attaatgcca cgtgtgccaa ccgagctgtt tattcgtgcc
     8641 tgtaaaaaag tggtaaaagc caatcaagat tggttagggc cttacggttc gggggcaacc
     8701 ctttatttac gtccgttttt aatcggcgtg ggcgaaaata ttggggtgaa agctgcacct
     8761 gaatttattt tttccgtatt ctgctgtccg gtgggggcat atttcaaagg tggcttagca
     8821 ccctcaaact tcattacaac cgactatgac cgtgcagccc caatgggaac aggtggtgtg
     8881 aaagtaggcg gtaactacgc tgcgagttta ttaccgcacg aattggcggt tgagcaaagt
     8941 tcaactaccc gtaaatttgc cgatgcgatt tatttagatc cgaaaaccca taccaaaatt
     9001 gaagaagtgg gggcggcgaa cttctttggg attaccaaag acaacaaatt catcaccccg
     9061 atttcagagt cgattttgcc aagtattacc aaatactcat tgctacacct tgctcaagag
     9121 cgtttaggta tggaagcgat tgagggagat gtgtatatcg accagttaga ccaatttgcc
     9181 gaagccggtg cttgtggtac ggctgcggtg attacgccgg ttggtggtat tcaacataaa
     9241 gacaaattcc acgtcttcta ttcagaaacc gaagtggggc ctgtcacccg ccgtttatac
     9301 gcagaattaa ccggtattca atttggcgat gttgaagcac cgcaaggctg gattgtaaaa
     9361 gtagagtaat tttacacaag taacaagcgg tcgaattggc aaaacttttt gcaaattcaa
     9421 ccgcttgtct ttatttagag aatatgaaaa agttaatttt tttaggttta tcagcacttg
     9481 cattagctgg ttgttcctca acaatttcta atcctaatca actgattggg caatggcaat
     9541 gcactattga gtacgatgat tttaatattc gtacggtgga taatttacga ttttccacaa
     9601 atgggactat gaccaatcaa ggtggtatat attaccctat tcaaaagtcg atttttgagt
     9661 attcattaca acagaatggg cgctggagtg tgaaaaacgg ggctattgtt tatcgtatcg
     9721 tctcagaatc tgtacagcgt agccatcgtt cacaaatatg gtctgaatta caacgtgatg
     9781 cggagctaca acaatttgaa gagaatttat ttagctcact ttctgatagt gataataata
     9841 aaacagtgga actaatcatc actgattttg atgaaaaaga aatggatatc aagcaagaaa
     9901 taaaagggca taaagcttat aaagggcaat gtataaaaca gtagaggtaa aaatgattca
     9961 acgttttgaa gtaggtagcc gtttttcgga aatggcaata cataacggtg tggcttactt
    10021 ggcagggcaa gtgccgttag atgacaccgc cgatgcttac acccaaaccc agcaagtgct
    10081 tgcagaaatt gataaatggc tggcaaaagc caacagcgat aaaagtaaaa ttctaatggc
    10141 tacggtttat ttaaaagaga tggcagatta tgcggaaatg aaccgtgcgt gggacgaatg
    10201 ggttgcccca aataacgctc caccaagagc ggcggtggag gctaaattag ccaatccaaa
    10261 ttggaaagtc gaaattgtgg ttaccgctgc ggtataaatg aaaagcagaa agacgatctc
    10321 tgtttttcat tatgattcgt acattttttc gatttgttgc ttatagcgtt ctagaatgac
    10381 ctttcgtctt aattttaatg taggcgtaat ttcttccatt ttggtgctaa atgcttgcga
    10441 aagtagtgta aactttttga tctgttcaaa acttgcaagg tctttttgta attcgttaat
    10501 gcgttgttca aacattctga caacctcata atgtttaatg agttctaatc tgtcttgata
    10561 gcgaatattg agcttttttg catactcttc gaggctatca aagcaaggaa ctatcaacgc
    10621 agaaacatat tttttggcat cagcaatcac cgcaatttgc tcaataaact tatctttacc
    10681 tatttttcct tcaatgtatt gtggggcaat atatttaccg tttgaagttt tcattaattc
    10741 tttaatacga tcggtaatat aaagattgcc ttccgaatcc agttcaccgg catctcccgt
    10801 ttttagaaaa ccttcagcag taaaggcttg agcagtggct tccggttttt tgtagtaacc
    10861 tttcataacc aagcctccac gcactaaaat ttcattttct tcactaattt tgacctcaac
    10921 atcaggcatt aaggtaccgg ttgagttgat attaaagcct ttttcctgcc agcatgaaat
    10981 agtggcagta gtttcggtca ttccgtatcc aagttttaca ttgatgccta tactttggaa
    11041 aaatagcccg atagacggat ctaatttagc tcctccacac ggcatcatac gaatacgacc
    11101 gcctagtaag gctcgtaatt ttcctagcac caatttatct gccataatgt actgcaagcg
    11161 gtggaaaaag gctgattttt taccaaacag attgtcttcg gcggtacgga ttgcccattt
    11221 aaataacgct cggcgatgta tcggtgcttg ttggacttta tcccacaccg cagtatagat
    11281 tttttcatag aaccttggta cggcacacat taatgtaggt cgtaccgttg agagtgcttg
    11341 acgtacctgt tgaggatctt ccagatagca atttactgcc cctctgtata gcacataagt
    11401 gacccaagca cgttcaaaga tatgcgataa aggtaaaaat gaaagggata tatcattttc
    11461 gtcaatatga gggagtgctt tattatgtgc ctgaagttga tgagctaaat tagcataatc
    11521 taacataaca cctttaggct caccggttgt tcccgaggta taaattaaag tgaataaatc
    11581 ctctaagcat ttatcattta aacgtttggt taattcagct tcatcttgat taaccttaat
    11641 gaaatcttgc caatgttgag cgatagggaa atcacgcaga tctattgagt ctttcatcgc
    11701 gacaattttt attaatagcg aacagttgtt cgctatttct atggcttgat catattgttc
    11761 ctgatcgcca acaaagagga tttttatatc cccgtcattc aggatatatt caagttgttt
    11821 ggtggtactg gttgcataaa tcggcactgc taccgcacga atttgcattg cagcaatatc
    11881 ggtaattgtc caacgaggca tattttgtgc aaaaatagcg attttctctt gaacggcaag
    11941 accgtttgcc agcaaggcat aagaaagatt attgacctgt ttttgtaatt ctaaccaact
    12001 tatttctgac catttttcat cttgcataaa acgtaaagct gggtggtggc taagagcctg
    12061 aacttgctgg tggaaacggt ggataaagtg aaaatctaaa gtgctcataa tcttattttt
    12121 actttctatt tcagtgaaca gttgtacgct aatataatag aaaatatgaa aaaaagaaac
    12181 agttgtactc tgaaacaaag ggttttggat aggaagtaat caaggcaata gaaatagata
    12241 agtagggtat cgtttagtac aaatgcaata tttttcaaaa atgagaccgc ttgtatatgc
    12301 aacaagcggt ctgttttgtt aaaaattttg caactaaatt cgttcaatat caaattgagc
    12361 taaatcaatc acatcaggtg taccgtaaaa agcacagagg gctttacgca aggtttcagc
    12421 tcgtttggga aggtagccac gttctgcata gagcttgact tggtcgtaca ctgcttgttt
    12481 aaaggctgaa ttatcgccgg gattgcccga gagattgtcg gcactagcaa aataacgaaa
    12541 accggcagca gttgctaaca cccattcaat cgcttgcggt ttgatttccg cactttcaaa
    12601 ttcacgttgg cgttcggcag agcgtccgtc cggctcgtac caatagccaa aatcttcaag
    12661 tttacgccga gcttctccag caacgagcca atgggcaatt tcgtgcaagg cactgctgta
    12721 atagccgtga gcaaataaaa tcacattgta agggcgttcg ctcggcacac catcttcgtc
    12781 cataaattcg ggcaagtaaa tcggataatt acccccccgc tctaggcgag tgttatattc
    12841 atcagaaaag cattgattaa aaatgcgaat aatgtcttca attttatgat tttcttgatt
    12901 catttctttt ctttataact tattcagcca agctaaaaat tgctcggcat ccaaaaagcc
    12961 agtaacacga gattggctca tttcattgcc gttttcatca aaaaacagaa tcgttggcaa
    13021 acctaataca ttaaagtgtt tcatcagctc gatattttca gccgagtttt tcgtcatatc
    13081 gatttgcaac actaccattt cattcagttt gttctgaaca ttttggtcac taaaggtgta
    13141 tttttcaaac tctttgcagg cgacacacca gtcggcatag agatcgagca tcactttttt
    13201 acctttggaa gcggtcaatt ttgtctctaa atcggcaagt gattgaattt tttctaacgc
    13261 taagtgcggt gtagtttgct gttgagtggt tgagccgttc caagctaaat ctgcccaagg
    13321 tttagcggca atggctaaag cgagcaataa taagactttg atgatctttt tcgtgattga
    13381 attagatgga atcgcatcca ccaaccagat caaaaatgca accgcaaggg ccgaccataa
    13441 aaacggttca tagtggcttg gcagcacacg gcttaataag aacaccggta aggcaagcat
    13501 aacaaaaccg aatgcagctt tcacttttaa cagccattcg ccggattttg gcagaatttt
    13561 gttgccgaat aaggtaatca aaatcagcgg taagcccatt cccagtgcga gtagatagag
    13621 ggctaagcca ccggtgacta aatcgccact ttgtgccaca taaagcaacg caccggaaag
    13681 tggggcagaa gtacaagggg aagcaattaa gcctgcgatc atccccatta aaaacacgct
    13741 acccaacgca ccaccttgct gtttttggct cattgcattg agtttttgtt gccagctatt
    13801 tggtagctgg atttcgtata agccaaacat tgagcaggca agcagaataa agacgataga
    13861 taaaccaatc aacaccggtg ggctttgtaa ggcgatttgg aaaggcaagc cgatagccgc
    13921 tacaattaag ccaagtaagg tgtaagtaac tgccattcct tgcacatagg caatacttaa
    13981 cagcaatgct ttagcggtag aagggcgttg cttatttcca attacaatcg cagagagaag
    14041 tggtagcatc ggcagtacgc aaggagtgaa tgctaatcca acgcctaata caaaaaacca
    14101 aaaaatggaa aaacgattgt tagctaaatt ttcagccagt tgatcttgct ctgcttttgc
    14161 cacattttca gggggcgcca tattgtcggt ttttgcaaaa ttttcgctct tttcgaccgc
    14221 ttgtgcttcc agttgcgcca gttcttcagg tgtagcagag agaatactgt ctaatttaag
    14281 ctcaacccgt tccggtggat agcaaaagcc tttggtacac ccttgatatt ccactacgat
    14341 tgaggcgttg tctttaattt ctttaaacgg tacctttagc cctaagtggt tgcgatagat
    14401 ttccacctgc ccgaaaaatt catcgttata ttgctcggca gcgggaaaag tgatgtcgcc
    14461 tatctcaata ttctgtggcg tgacacggat ttctttttta taaagataat aattgtctgc
    14521 aatatcccaa tttaactgta atgtttcatt ttcattttgg attgaaaaaa caaaggcttc
    14581 ttccgatttt aggaatttgg gttgagagaa aagatttgcg tgagatgagt ttatcatcaa
    14641 taaactcaag acgagtgaaa aaaagtatct aatcatattg aaatcctatt tttaattgtc
    14701 tcggcgaaaa ttatagcgta atcagtattc atattccaaa taaattacgc catttaacga
    14761 tgttagacga tgtaagtgct attttattac aaaaattttg ttc
//
LOCUS       DANLXQ010000053        14714 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.053, whole genome shotgun sequence.
ACCESSION   DANLXQ010000053 DANLXQ010000000
VERSION     DANLXQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14714
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.053"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            70..282
                     /locus_tag="PXD94_001214"
     CDS             70..282
                     /locus_tag="PXD94_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6286087.1"
                     /translation="MTNKKIQKAIELVGTQTKLAEACGVSQPTVLKWLNGGGISAEYL
                     LKIEAATNGQVTIREICEELGNATAK"
     gene            complement(381..626)
                     /locus_tag="PXD94_001215"
     CDS             complement(381..626)
                     /locus_tag="PXD94_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286088.1"
                     /translation="MNTEDQIIELTVEVENLRHQLNAKNIILDDLKGRLTDYQRKRLD
                     RLFERSRRQYLNDLDLEEEQAEALEAICNELESFLAN"
     gene            759..1031
                     /locus_tag="PXD94_001216"
     CDS             759..1031
                     /locus_tag="PXD94_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286089.1"
                     /translation="MNLDHTCTNCKSKNLRVRTSEKIGLLTIQAVTYCNNCGTEQLVQ
                     SQIIRVRTPIYKERPEAMRINKCLMEADLNTPDLFDGVVADQPKQD"
     gene            1114..1446
                     /locus_tag="PXD94_001217"
     CDS             1114..1446
                     /locus_tag="PXD94_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286090.1"
                     /translation="MSKQLYYRHKSRWNRLNRLKKMNKRRLNLFLQEKRVANLESRLA
                     DEVELAKLNSEQIGDLLQALELKVNRLIRQNEKLKARVIKLEKVKQTQKQGLFAMFKK
                     VLGSKGNP"
     gene            1459..1752
                     /locus_tag="PXD94_001218"
     CDS             1459..1752
                     /locus_tag="PXD94_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286091.1"
                     /translation="MDFVATYQKIRINTNHKQFTCNHLGEIWGKENDDFKRFLLRVAF
                     LQDNPEPKPWGAYSPQEKRLLHSFSLWLEQIADFQRVMKRESKLREKHINAPD"
     gene            1904..2146
                     /locus_tag="PXD94_001219"
     CDS             1904..2146
                     /locus_tag="PXD94_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286092.1"
                     /translation="MSLITVNDYKIKKDILDADGKPCYQLYQGEELLRDTTNLSEIIA
                     YMFIRAEDKPFDELYNHIINQEAGAKTDIKFTVTVR"
     gene            2143..2475
                     /locus_tag="PXD94_001220"
     CDS             2143..2475
                     /locus_tag="PXD94_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286093.1"
                     /translation="MSQITKSAYTHIHPNWQAPRGNTYRIERYELKRRYALFCNGERV
                     VLYKDPTHPNKNVESHIESDVLLLWLLKNELGWSGNIKEPLSPQDVENIRLLEQAFCL
                     TYNGTFIK"
     gene            2472..4838
                     /locus_tag="PXD94_001221"
     CDS             2472..4838
                     /locus_tag="PXD94_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDL6286094.1"
                     /translation="MNYQNEILSATEAFAIGVSLPPVAGASACALELHSDRLYQAFKQ
                     PYTSMVQQELFSKDPIHEPLRQSYFERLPRALAEHFGRQYKQKLHYQSQYDTADWFKA
                     EMARKMPRIEAVISQYCDVFDFLKQSHQDLDFLAELDSNVYFGAGVDTQQAVQKIKQD
                     AEVKGIQLPMHLCRLENSSRYLKAHGIRPLAYQTEEQIKQLALGIAYRVRQIQQDNIE
                     QNMHKATDGDTAYSVLLECYRAMMGEVNKLKIDSPYQKKARKGRLTEEEITTGFLKMT
                     CEKWWTRKLSKIAEQMKEHLAIACGMVNMLSPYCSNARLKAFEAQRKANIDYLKSMII
                     ANIAEPEEQLSLFETWLKSASNPKIKRLELLTRMNGFERYADKQGHEGWFITLTAPSK
                     YHAMLSRTSSVNPKWNGASPAETQVYLVNTWAKIRAKLNREGVMAYGFRVAEPHADAT
                     PHWHLILFTRPEDMEKLRRVFLSYALEVDGTEAGAKKYRCKFKRIEKEKGSATGYLVK
                     YVSKNIDGFGMDGELSDEANIQAKENAARVGAWSSVWCIRQFQQLGNIPISLWRELRR
                     LGSVEQEDETLEKLRVIADSGEWDVFTEELGGALVKRADLVARITYTERKSETGEALY
                     TMYQEPSLKVSGIINIKNGVQINTRPKEWSIQLKPKNWEEQQLQKKLENATETERAEV
                     KRKYYEKLGFNEEAIALLGELAPPWTWTCVSNCTGSKNNQISEEARNCLRNELITMRG
                     RVTEHQIDDLLNGKRLKIWGNSQKTMFVSYSRGRLIEHIVDNYESFLN"
     gene            4851..5183
                     /locus_tag="PXD94_001222"
     CDS             4851..5183
                     /locus_tag="PXD94_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286095.1"
                     /translation="MVKFIRLNLINYFIEKDEYGEPVRCRAFRYDEIIQTKFIEGFRE
                     GEIPADDYGFEDWNGSVLILNEEGRKYFENYLFDVKKNYCRARDNYVYVLETLDEIEM
                     KLGLLEAK"
     gene            5183..5635
                     /gene="ssb"
                     /locus_tag="PXD94_001223"
     CDS             5183..5635
                     /gene="ssb"
                     /locus_tag="PXD94_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6286096.1"
                     /translation="MNLVTLVGRLGQDPDIRTMQNGEKAAALSVATSEKWTDKQTGVK
                     KESTEWHRVVLYRRLAEIAELYVKKGHLVSIIGKIKTRKWTDSNGVERSITEIIAEQM
                     QMLSSGEKNTPNTPNKAENKPQPKQKNQDVMTADEQKDIPQFDDDIPF"
     gene            5646..5978
                     /locus_tag="PXD94_001224"
     CDS             5646..5978
                     /locus_tag="PXD94_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286097.1"
                     /translation="MREWEKQMPFSANLHIRPTGKFEVKNVNIEQIIGERGKNYGNFD
                     DVATMSQQLKAVLLDTNTQLKPHQKEAGEMICLKLARIFAGCNPDYEDNWRDIAGYAV
                     LGGKLNEK"
     gene            5968..6237
                     /locus_tag="PXD94_001225"
     CDS             5968..6237
                     /locus_tag="PXD94_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286098.1"
                     /translation="MKNKMGSKFIRVRVEHPQRKKEDIELVQDVVAYHIFNLFKFEES
                     GLRGFSQYDLEIDVKVKPVLEQKEMPVDVWIDDLGGKNGTITSGQ"
     gene            6212..6502
                     /locus_tag="PXD94_001226"
     CDS             6212..6502
                     /locus_tag="PXD94_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286099.1"
                     /translation="MEQLQAVSNEQVFAKLCEVERLLKTKGVNEHSRELWDLGDVAAY
                     FGYTKEHTSRSVVSSPHFPKPIALDGLRGKGRGAKKWVSGEVVKFCLMWKVK"
     gene            complement(6772..6954)
                     /locus_tag="PXD94_001227"
     CDS             complement(6772..6954)
                     /locus_tag="PXD94_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDL6286100.1"
                     /translation="MAMSRNEIQAKSEAKRGIKQKSFKLPLEVIAEIEVLSQKLNIPQ
                     NQLIIQAIQQFKQNNP"
     gene            complement(7232..8230)
                     /locus_tag="PXD94_001228"
     CDS             complement(7232..8230)
                     /locus_tag="PXD94_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6286101.1"
                     /translation="MATIIKNGNKYRAQVRKKGIYKTATFSTKTEANRWAYALEAQID
                     AGEYNTTPNILFAELIDKYLKEVTPTKRGKREERYRLLRIAGSNLGNIPLPALTKEHL
                     RQWQNQRLQEVQPVSVSRERATLSALFTKAMEWDYLKENPLKTLEKIKTPPARTRRYS
                     DEEIDKLIFVSGYSPNQPPLLMQQRAGAALLFAIETAMRAGEIAGLKWEHINLQARTA
                     FLPQTKNGYSRTVPLSTKAIAILNQLAQLEPNKQNSVFQLTSRSLDSNFRKLKQKANL
                     HNADLHFHDTRREALTRLSKYLTVMELAKVSGHRDLSILQNTYYNPDISELVSKLQ"
     gene            complement(8458..8534)
                     /locus_tag="PXD94_001229"
     tRNA            complement(8458..8534)
                     /locus_tag="PXD94_001229"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8498..8500),aa:Val,seq:gac)
     gene            complement(8598..9398)
                     /gene="apaH"
                     /locus_tag="PXD94_001230"
     CDS             complement(8598..9398)
                     /gene="apaH"
                     /locus_tag="PXD94_001230"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL6286102.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLGIKRVKPNDKIDAIFEAKDRLDLQNWLRNQP
                     LVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKPERWS
                     EDLQGVERWRYIINVFTRMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLFTQKN
                     IIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            complement(9490..11466)
                     /locus_tag="PXD94_001231"
     CDS             complement(9490..11466)
                     /locus_tag="PXD94_001231"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL6286103.1"
                     /translation="MFQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYF
                     IPPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVD
                     HPSIKQTIPANTHKKATEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAP
                     WWVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIK
                     QNGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCS
                     LVPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQ
                     SEETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQ
                     MIEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELA
                     ERYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYA
                     TWTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVLSRLQEARRQNRLVERDIADWLYA
                     RYLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELA
                     LYIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
     gene            11626..11904
                     /locus_tag="PXD94_001232"
     CDS             11626..11904
                     /locus_tag="PXD94_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL6286104.1"
                     /translation="MKKLLAIATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGLAQ
                     KQEIDAAKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            11929..12225
                     /locus_tag="PXD94_001233"
     CDS             11929..12225
                     /locus_tag="PXD94_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL6286105.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
     gene            complement(12278..13069)
                     /locus_tag="PXD94_001234"
     CDS             complement(12278..13069)
                     /locus_tag="PXD94_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6286106.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPLDVATDESITECFTELSKHWEKFDGFVHAIAFAPGDQLDG
                     DYVNAATREGYRIAHDISAYSFVAMAQAARPFLNENAALLTLSYLGAERAIPNYNVMC
                     LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRR
                     TVTIDDVGNSAAFLCSDLASGVTGEVLHVDAGFSVMAMGELGDEE"
     gene            complement(13213..13986)
                     /locus_tag="PXD94_001235"
     CDS             complement(13213..13986)
                     /locus_tag="PXD94_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HDL6286107.1"
                     /translation="MKIVSEPKTSGRHTCTIHCQNCSISQLCLPFTLNETELTQLDNI
                     IERKKPIQKSQVIFKLGDELRSLYAIRSGTLKAYTLSESGEEQITGFHLPGDLIGFDA
                     ITNMQHEGYAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
                     SKMNAEEKLAAFLYNLSQRYSARGFSAKEFRLTMTRGDIGNYLGLTIETISRLLGRFQ
                     KSGMISVQGKYIIINRMEELAELAGAIKPQQNIIAQTTA"
     gene            14217..14642
                     /locus_tag="PXD94_001236"
     CDS             14217..14642
                     /locus_tag="PXD94_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL6286108.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQYSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRRVMNNITVDMLVVPLADE"
BASE COUNT         4445 a         2604 c         3284 g         4381 t
ORIGIN      
        1 catttggtta ttgatttttt atttctttgg ttatattatt ataaccattc aataaccaga
       61 gttatattta tgactaataa gaaaattcag aaagctattg aacttgtagg aacacaaaca
      121 aaattagctg aagcttgtgg agttagtcag cctacggtct tgaaatggct aaacggaggc
      181 ggaatttccg ctgaatatct actaaaaatc gaagctgcca caaacgggca ggtaacaatt
      241 cgtgaaattt gtgaggagtt gggaaatgca acagcaaaat aaaagccctg aacaggcagg
      301 gctagcgagt gggaaattta ctgtaagacg taatcaagac ggtaaatggc gtttagattt
      361 gaccttgttg gattttggta ttagttcgcc agaaacgact caagctcgtt gcaaattgct
      421 tcaagagctt cagcttgctc ttcttcaagg tcaaggtcat ttaaatactg tcggcgtgaa
      481 cgttcgaaaa gccggtccag tcgtttgcgt tggtagtctg ttaagcgacc ttttaaatca
      541 tcaagaataa tgtttttggc gtttagttga tggcgtaagt tttcaacttc aacagtcagt
      601 tcaataattt gatcttcagt attcataaaa tttccttttg gagggttaaa caatatctaa
      661 aacataacca aaattcgact gcttgtctat ggggatagat aagccgaaat caacgtaaag
      721 aatgttaata gagccttggg gaaggcgtag gggaaataat gaatttagac cacacctgta
      781 caaattgcaa aagtaaaaat ttacgggttc gtacatctga aaaaatcggt ttgctgacta
      841 ttcaggctgt tacttattgt aataactgcg gcacagagca acttgtgcaa agtcaaatta
      901 tccgtgttcg cacaccgatt tataaagagc gaccggaagc aatgcgtatt aataaatgct
      961 tgatggaagc cgatttgaac acgccggatt tgttcgatgg cgttgtggca gaccaaccta
     1021 agcaagacta atcccaattt ttaaacaatc gtcaaaataa gccttatttg catagggcga
     1081 gatttttgca ccctaaattc aggaatttga acaatgagta agcaattata ttaccgacac
     1141 aaatcacgtt ggaacagact taaccgcctt aagaagatga ataaacgtcg ccttaatctg
     1201 tttttgcaag aaaagcgggt ggcgaattta gaaagccggt tagctgatga agtagagctg
     1261 gcaaagctga atagtgagca gattggcgat ttactgcaag cgttggagtt aaaagtgaat
     1321 cggttaattc gccaaaatga aaagctgaaa gcccgtgtga ttaagctgga aaaagtcaaa
     1381 cagacccaaa agcaaggttt gtttgcaatg tttaaaaagg tgttgggtag caagggtaat
     1441 ccttagtaag aggtagatat ggatttcgtg gcaacgtatc aaaagatacg cattaacact
     1501 aatcacaagc aatttacttg caatcactta ggcgagattt gggggaagga aaacgacgat
     1561 tttaaacgtt tcttactacg agtcgcattc ttgcaggata accccgagcc gaaaccttgg
     1621 ggagcgtatt ccccacaaga aaaacgcctt ttacatagtt ttagcttgtg gttagagcag
     1681 atagcagatt ttcagcgagt aatgaaaaga gaatcaaaac tgcgtgaaaa acatattaac
     1741 gcaccggatt agaaggagta aatatgagcc gtaaccgtga attgtgtgag ttttttcaca
     1801 aactgtggtg caaaaccggt aatcgctatg cttatcgccg gtatcaagtg ttttttcagc
     1861 gttatcaaaa cgaatgttta gtgaatgagt agagggtaga aaaatgtcat taattactgt
     1921 gaacgattac aaaatcaaga aagatatttt agatgcagac ggcaagccgt gttatcagtt
     1981 gtatcaaggc gaagagctat tgcgtgatac caccaattta agcgaaatca tcgcttatat
     2041 gtttattcgt gccgaagata aaccgtttga tgagttgtat aaccatatca tcaaccaaga
     2101 agcaggagct aaaacagata ttaaatttac ggtaacggtg agatgagcca gattactaaa
     2161 tccgcttaca cacatattca cccaaactgg caagccccga gaggtaacac ttaccggatt
     2221 gagcgatatg agctaaaacg gcggtatgcc ttattttgta atggtgagag ggtagtttta
     2281 tataaagacc caacacaccc caataagaac gtagagagcc atatagaaag tgatgttttg
     2341 ttgttatggc tactgaaaaa tgaattaggg tggagcggta atattaaaga gccgttaagc
     2401 cctcaagatg ttgagaatat ccgcttgtta gagcaagcat tttgtttaac ttataacgga
     2461 actttcatta aatgaattat cagaatgaga ttctatctgc tacagaggct ttcgcaatag
     2521 gtgtttcact cccacctgtt gccggtgctt ctgcttgtgc attagagctt cattctgatc
     2581 gtttatatca agcgttcaaa cagccttaca cttcaatggt ccagcaagaa ttattctcga
     2641 aagaccctat tcacgaaccg ttacgccaat cttattttga gcgtttgcct cgtgccttag
     2701 cagagcattt cggcagacag tataagcaaa agctacatta ccaaagccaa tacgacacag
     2761 cagactggtt caaagcggaa atggcacgca aaatgccacg cattgaggca gttattagcc
     2821 agtattgcga tgtattcgat tttctgaagc aatcccatca agatttagat tttttagctg
     2881 aattagatag taatgtttat ttcggtgccg gtgttgatac gcaacaagcg gtacaaaaaa
     2941 ttaaacaaga tgccgaagtg aaaggtattc aactgcctat gcacctttgc cgtttagaga
     3001 atagctctcg ctatttaaaa gctcacggca ttcgcccgct tgcttaccaa accgaagagc
     3061 aaatcaaaca gcttgcctta ggtattgctt atcgtgttcg ccaaattcag caagataaca
     3121 tcgagcagaa tatgcacaaa gcgacagatg gcgacacagc ctatagtgtg ttgcttgaat
     3181 gctatcgggc aatgatggga gaggtaaata agctcaagat tgattcgcct tatcagaaaa
     3241 aagcgagaaa gggtagatta actgaagagg aaattaccac aggcttttta aaaatgacct
     3301 gtgaaaaatg gtggactaga aagttatcta aaattgccga acagatgaaa gagcatttag
     3361 ctattgcgtg cggtatggtg aatatgcttt cgccttactg ttcaaatgct cgcttgaaag
     3421 cgtttgaggc tcaacgcaag gcgaatattg attatttgaa atcaatgatc attgccaata
     3481 tcgccgagcc ggaagagcaa ctttcattat ttgaaacgtg gttgaagtct gcctctaacc
     3541 cgaaaattaa acgccttgaa ttactcactc gtatgaatgg atttgagcgt tatgcagata
     3601 agcaagggca cgaggggtgg tttattacct tgactgctcc gtctaagtat cacgcaatgc
     3661 tttcaagaac cagtagcgtg aacccgaaat ggaatggagc aagccctgcg gaaacacagg
     3721 tttaccttgt gaatacttgg gcgaaaatcc gtgccaaact caaccgtgag ggcgtaatgg
     3781 cttacggttt tcgagtggct gaacctcacg ctgacgctac cccacactgg catttaattc
     3841 tattcactcg tccggaagat atggaaaagc ttcgccgcgt gttcttgagt tatgccttag
     3901 aggtcgatgg cacagaagcc ggtgcgaaaa aataccgttg caaattcaag cgtattgaga
     3961 aagagaaagg ctcggcaacg ggctatttag tgaaatatgt ttctaaaaac attgacggtt
     4021 ttggaatgga cggcgagctt tcagacgagg caaatattca ggcgaaagag aatgccgctc
     4081 gtgtaggagc gtggtctagc gtttggtgta ttcgacagtt ccagcagtta ggtaatattc
     4141 cgatcagctt atggcgtgag ttacgccgtt tggggagtgt agagcaagaa gatgaaactt
     4201 tagagaaact ccgtgtgatt gcagatagcg gtgagtggga cgttttcacc gaagaattag
     4261 gcggtgcgtt ggttaaacgt gccgatttag ttgcacgtat tacttatacc gaacgcaaaa
     4321 gcgaaaccgg tgaggcgtta tatacgatgt atcaagagcc atcattaaaa gtgagcggca
     4381 ttattaacat taaaaacggt gtgcagatta atacacgccc gaaagagtgg agcattcaac
     4441 ttaaacctaa aaactgggag gagcaacaat tacagaaaaa attagaaaat gcaaccgaaa
     4501 cggaacgagc ggaggttaaa cgtaaatact acgagaagtt agggtttaac gaagaggcta
     4561 tcgctttatt aggggagctt gctccccctt ggacttggac ttgtgtcagt aactgtacgg
     4621 ggtcaaaaaa taatcagatt agtgaagagg ctcgaaattg cctaagaaat gaactgatta
     4681 caatgcgagg acgggttact gaacatcaaa tagacgattt actaaacggc aagcggttaa
     4741 aaatttgggg aaattcgcaa aaaacaatgt ttgtgagtta tagccgtggg cgtttaattg
     4801 agcatattgt tgataattat gagagttttt tgaattagga tattaagaaa atggttaaat
     4861 ttattcgttt aaatttgatt aattatttca ttgagaaaga tgaatatgga gaaccggtgc
     4921 gttgcagagc gtttcgttat gatgagatta ttcaaactaa gtttattgaa ggatttcgag
     4981 agggagagat accagctgat gattatggat ttgaagattg gaatggttcg gttctgattt
     5041 taaatgaaga aggacgtaaa tattttgaga actatctttt tgatgtaaag aagaattatt
     5101 gtagagcaag ggataactat gtatatgtct tggaaacttt agacgaaatt gaaatgaagt
     5161 taggattatt ggaggcgaaa taatgaactt agtcacatta gtcggacgtt tggggcaaga
     5221 ccctgatatt agaacaatgc aaaatggtga gaaagctgcg gctttatcgg tagcgacctc
     5281 tgaaaagtgg acagataagc aaacaggtgt gaagaaagaa agcaccgaat ggcatagggt
     5341 ggtgctttat cgccgattag ctgaaatcgc cgaattgtat gtaaaaaaag ggcatttggt
     5401 atcaattatc gggaaaatta aaacccgaaa atggacggat agcaacggtg ttgagcggag
     5461 tattacagag attattgccg agcagatgca aatgctcagt agtggtgaga aaaatacgcc
     5521 aaatacgcca aataaggcag aaaataaacc gcaaccaaag cagaaaaatc aagacgtgat
     5581 gactgcggac gagcagaaag atattccgca gtttgatgat gatatcccgt tttagggggt
     5641 gaataatgag agaatgggaa aaacaaatgc catttagtgc aaatttacat ataagaccta
     5701 caggaaaatt tgaagtgaaa aatgtaaaca tagaacaaat aatcggagaa cgtggcaaaa
     5761 attacggcaa ctttgatgat gttgccacaa tgagccagca gttaaaagca gtgttgctgg
     5821 ataccaatac tcaacttaaa ccacatcaaa aagaggcagg cgagatgatc tgcttgaaac
     5881 tggctcgcat ttttgcaggt tgtaatccgg actatgaaga taactggcga gatattgcgg
     5941 gttatgcggt gctgggaggg aaattaaatg aaaaataaaa tggggagtaa atttatacga
     6001 gtcagagttg agcatccaca gcggaaaaaa gaagatattg agcttgtgca ggatgtggtt
     6061 gcttatcata tttttaatct ttttaaattt gaggaaagtg ggttacgggg gtttagtcag
     6121 tatgatttgg aaattgatgt gaaagttaag ccagtgttag aacaaaaaga aatgcctgta
     6181 gatgtttgga ttgatgattt aggtggtaaa aatggaacaa ttacaagcgg tcagtaatga
     6241 gcaagttttt gcaaaacttt gtgaggttga gcggttatta aaaaccaaag gggtgaatga
     6301 gcatagccgt gaattgtggg atttgggcga tgtagcagca tatttcggct acactaaaga
     6361 acacaccagc cgaagtgttg tatctagtcc acattttccg aaaccgattg cccttgacgg
     6421 cttacgaggc aagggcaggg gggcgaagaa gtgggtatcc ggtgaagtgg tgaaattttg
     6481 tttgatgtgg aaggtgaagt gatgataaaa aagataattt tagatgcttg ttgcggtagc
     6541 cgaatgtttc actttaataa gcacaacccc tcggtattgt ttgctgacaa tcgggaatcc
     6601 ctgtatcgaa aattttaaat gttatcgaat accaacctat ctatgggcat aaaagcggta
     6661 aacataacaa aacgcattgg ctggcatttg taaagatgga taataacgat tatttgtaaa
     6721 gtatttcaaa atacaaaagc cgataaattt cattatttat cggcttttgt tttatgggtt
     6781 gttttgcttg aattgttgaa tagcttgaat aatcagctgg ttttgtggaa tgttgagttt
     6841 ttggctaagt acttcgattt ctgcgatcac ctcaagcggt agtttaaagg atttttgctt
     6901 tattcctcgt tttgcctcgc ttttagcttg gatttcattt cttgacattg ccataattta
     6961 tccctattat aaattttgtt gaagagctgg gagctttcac ccccagcgag ttgcttcaga
     7021 ttagtaagct ggggagctaa ggactaataa gaagacaact aggataatgt atttaaacat
     7081 tgtgctatcc ttcttcattt cgtaaggttt aagccttacg ctcactttca agctgtctct
     7141 tgaaagtgag gttattataa tgtagggtat cctacattgc aagtaataaa tacaaaaaag
     7201 ccgataaatt ttcgtttatc ggcttttgtt tttattgcag tttgctcaca agttctgaaa
     7261 tatccgggtt atagtaggta ttttgcaaaa tacttaaatc tcgatgcccc gatactttgg
     7321 caagctccat taccgtcaga tatttagaca aacgtgtgag tgcctctcgc cgtgtgtcgt
     7381 gaaaatgcag atcggcattg tgtagattgg ctttttgttt cagcttgcga aaattcgaat
     7441 caaggctgcg actggtgagc tggaaaacac tgttttgctt gttcggttca agttgtgcca
     7501 actgatttaa aattgctatt gctttggttg agagtggcac ggtgcgtgaa taaccgtttt
     7561 tggtttgtgg caaaaatgcg gtgcgtgcct gcaggtttat atgttcccat tttaaaccag
     7621 caatttcacc cgctcgcatt gcagtctcaa tcgcaaataa tagtgctgct ccggctcttt
     7681 gttgcattaa tagcggtggc tggttagggc tgtagccgga gacaaaaatc agtttgtcta
     7741 tctcctcatc gctatatcgg cgagttcttg ctggtggcgt tttgattttt tccagcgttt
     7801 ttagaggatt ttctttgaga taatcccatt ccattgcttt tgtaaagagt gccgataaag
     7861 tggctctttc tcttgagacg gaaaccggct gcacttcttg caagcgttgg ttttgccatt
     7921 ggcgtaaatg ctctttggtg agtgccggta atggtatatt tcccaagttg ctgccggcaa
     7981 ttcttagcag gcgatagcgt tcttcccttt tgccacgttt ggtcggagtg acctctttga
     8041 ggtatttgtc gattaattcg gcaaaaagaa tgttcggggt ggtgttatat tctccggcat
     8101 caatttgagc ctctaaagcg taagcccagc ggtttgcctc tgttttggtg ctgaaagtgg
     8161 cagttttata aatgcctttt ttgcggactt gtgctcggta tttgttgccg ttcttaataa
     8221 tagtagccat ttttttattc ctttgtgtat gtgtgcagtt taagtgtgca aatgtgtgca
     8281 aaggaatgtt aatagagatc ataaacgatc aataaatatc aagttttcat atcaaaaatg
     8341 atgtttttat tgatgttatg ctacacttgg ggaagtgttt taaatcaata agctattgat
     8401 ttataagggg tatttttggg gttaagcctg aatggggata ttcaggctta aaaataatgg
     8461 tgcgactagc tggactcgaa ccagtgaccc ccaccatgtc aaggtggtgc tctaaccaac
     8521 tgagctatag tcgcattaac ggtgtgcatt ctattggaaa aaggaactta gtgcaatatg
     8581 cttttggtcg aatgctttta ttttagacgc ttttgggtaa aaatttgttt atcttcccaa
     8641 cggagcatgg ttaaatggct tccccaagca cagccggtat caagtgcata gatgtttgga
     8701 cgagtgcatt tgcccattag gcttgcccag tgaccaaaga taatgttttt ctgtgtaaat
     8761 aacggattat ctaagtcaaa ccaagccagc agttcttgag gggcttgttc aagcggtaat
     8821 ttacagtcaa aatctaaccg cttgtcggtg tagcaaaacc gcatacgggt aaacacattg
     8881 atgatataac gccaacgttc aacaccttgc aaatcttcac tccagcgttc gggcttattc
     8941 tcatacattt gggcgatgta gtccgcataa ttgtcacttt gtagcacctt ttctgcctct
     9001 ttggcacaag ctagggtagt tgctaaatcc cactcagggc tgattcctgc gtgagtcagc
     9061 aaaaattgat gtttggggtg atgaattaca agcggttgat ttctgagcca attttgcaaa
     9121 tcgagccgat ctttagcctc aaaaatagca tcaatcttat catttggttt tacccgtttg
     9181 atgccaagca agtgcaaatc gtggttgcct agcacggttt tggcattgtt tgcagagtct
     9241 ttcacaaaac gtaaacaggc taaagagtcc tcaccacggg cgactaaatc ccctgttaaa
     9301 aagagttcgt cttgcttggg gttataattg actttttcta ataaaagctg aaattcgtta
     9361 aagcaaccgt gtaggtcgcc tacaatataa gttgccattg tttctccttt gttcccctaa
     9421 agttataaac aagcggttat attttagaga aaatttgcaa aatctcatca aaatataacc
     9481 gcttgaaatt taaatgatat ttcctacgat tgagcgggtt tctaagcgta cttcggtgag
     9541 tttcactttc accgcttgcc cgatctgata ggctttttca cctttaatat aaagtgcaag
     9601 ttcttcttgg cggtattcca tttcctcttt gttgtcgtgc aagctggaga atggcacaaa
     9661 aatttgagca ccgtttttta tgactttcgc acgcaagcca ccacgggaaa catctgcaat
     9721 ttcgcactca aattcgaccg cttgttccac cattggggca aggtaacgag cataaagcca
     9781 gtcagcaata tcacgttcta ctaaacggtt ttgacggcga gcttcttgta aacggcttaa
     9841 aactgattct tccaccgctt ggctttgttt gccaagtaac acttgcttga ttaaacggtg
     9901 gtttaccata tcgccatatt tacggatggg tgatgtccaa gtggcgtaat cggtaatgcc
     9961 tagtccgaaa tgcggggcaa cttcggcttt aaattcagcg aaggttaaat aacgtcgtaa
    10021 acgcaactct aagaatttct ctgggtagtc ttgaatatca cgttgcattt ggcaatagcc
    10081 ttctaaggtt gctaaacgct cggcggagta gcgttcggca agctcatcac gattttcatc
    10141 gctggctaaa gtttggagta aaaatgattt taccaattca taatttttcg gatcaaaacc
    10201 tgaatgggta ttaaatacac cggttttggc atgttggctt aagaattttg cacaacaaat
    10261 attggcaaga atcattgatt cttcgatcat ttgattcgca atacggcgat attcaacgtg
    10321 aatatctttt accgagccat cttcatttaa ctcaaaggta taatcgcctt gttctttgaa
    10381 tagcaacgca ttctcggcac gccattgaat gcgggcttga gtaaattgat gtagccactc
    10441 aatttgttgt tttgtttctt cagactgagg ttgccacgca ttttccacgc cttcgagata
    10501 atcggaaaca tcgtcgtaag ccaagcgatc ttttgattcg acccaagcag aggtaaaggt
    10561 tgtctcttta atgagattgc cgtttaaatc ggtttcaata aagccaacga gggcagggcg
    10621 tttttcattc gggacaagcg aacagagatc gtctgagagt tctcgaggta acattggaat
    10681 gttaaagccc ggcaaatagt tggtgaaaca acgctggcga gcggcttttt caatatggga
    10741 attttctggg atataagcgg ttgggtcagc aatcgctacc actaaacgcc agccggttcg
    10801 cttgccattt tgcttaattg gttcgatgaa cagagcatcg tccatatctt gcgtgcttgg
    10861 gctgtcaata gtggtaaagt aaagatgaga taaatcttca cgagctaatt catcgtgtaa
    10921 ttcatagttt ttttcatttg cgaccggttc acgaggctgt tcgtggcggg caagtgtgac
    10981 ccaccaaggg gcaaaattat cagattcttt acagataaat tgggtaactt gagcaaagaa
    11041 aaaacggtcg tcacgcaatg ggtgcgtttt taattgtgca accacccagt cgccattttc
    11101 taatttttca gtggcttttt tatgggtatt tgccgggata gtttgcttaa tgcttgggtg
    11161 atcaaccgca agttgcaatt tgccctcttt attcaagcga acttgggcaa taaatctatc
    11221 cagcattggc tcaagtagtg aatcaatttc tacctgttct ttgtcgccat cacgtttgac
    11281 tacggctttc actgaatcac cgtgcattac ttttttcatt tccgcaggtg gaataaaata
    11341 gcttttttta tcgcattcta aaaaaccgaa tgctttgtct gaggctttga caacgcctgc
    11401 gacatattct ttgcttgctt caatttgttg tttgagttgg gctaaaaggg gattgttttg
    11461 aaacataatt tctcatttta aaaataaaaa aaggtgctat cgttgcaccg aaaaaaacaa
    11521 tggcattata acggatttga accaagttca gcaaataaat gctgttgcta taacttataa
    11581 aggttggtag aatataatcc atttaaaaat aatggagact tttcaatgaa aaaattactt
    11641 gcaattgcaa cagtattttc tttagcagcc tgttctactc aaacacttga actatctcct
    11701 gctaaggaag ctcaatatga tcgtgttcaa cacttctttg tttcagggct tgctcaaaaa
    11761 caagagatag atgcagcaaa aatttgtaat ggtgcagata aggtaggtaa ggtagaaact
    11821 gaaactactt tctttaatgg cttgttaggt acaattactt atggtatcta ttcacctcgc
    11881 caaatccgtg tatattgtaa ctaatcctag taatttaagg agttgaaaat gaaaaaattg
    11941 cttgctatgt ctgtattaac agccctgctg actacagcat gtacaacaca aactgcccat
    12001 attggcggca aagttactga taatattaaa ccaactaaga gtgtaactca gtcgttcttt
    12061 ataggtggga ttgggcaaga agaaacattg aatgttgtag aggtttgtgg ttccaaagat
    12121 aaagtgcagc aggttgaaac agtactttca ggtggggaca ttctattagg tcttgtgact
    12181 atcggtattt atacacctcg tactgcaaat gtatattgta agtaatattt tattaaaaaa
    12241 ataacggact caattgagtc cgttattttt ataggaatta ctcttcgtcg cctaattcac
    12301 ccattgccat tacgctgaag ccggcatcaa cgtgtaacac ttcacctgtt acaccagagg
    12361 ctaaatctga gcataagaaa gctgcagagt tacctacatc atcaatagtt acggtgcggc
    12421 gtaaagcagc ggttttctcg aaagcagaaa gcattttctt gaagttttta atgcctgaag
    12481 cagctaatgt acggatcggg ccggctgaaa tcgcatttac acgaatacct tctttaccta
    12541 aatcggctgc cattacacgg gttgcagctt ctaaagaggc tttggctaag cacattacat
    12601 tgtagttggg gattgcacgc tctgcaccta agtaacttaa ggttaaaagg gcggcatttt
    12661 catttaagaa ggggcgagca gcttgagcca ttgcaacaaa gctataggca ctgatgtcgt
    12721 gtgcaatacg ataaccttca cgggttgcgg catttacata atcgccgtct aattgatcgc
    12781 ccggtgcaaa tgcgatagcg tgaacaaaac cgtcaaattt ttcccaatgt ttgcttaatt
    12841 cagtaaagca ctcagtaatg ctttcatcgg ttgctacatc taacggaagg acaatgtctg
    12901 agccaaattc tttggcaaat tcttcaactc gtggttttaa tttatcgttt aagtaggtga
    12961 atgcaagttc tgcaccttgt tgcttcattg cgtttgcaat accgtaagcg atcgaacggt
    13021 tgcttgctaa acctgtcact aaaatacgtt taccagttaa gatacccata aaaattcctc
    13081 ttttatattg attggggcaa aattgtgtcg attataacgg atataggaaa aacgggcaaa
    13141 tggagttagc ggctcatcgg atcagaaatc cctttttgaa cgaggtttca aaaagggatt
    13201 tggtaagata aattaagcgg tagtttgagc aataatattt tgctgcggtt tgattgcccc
    13261 ggctaattcg gctaattctt ccatacggtt aataataata tattttcctt gtactgaaat
    13321 cataccgctt ttttggaaac gccctaataa acggctgata gtttcaatgg ttaaaccaag
    13381 ataattgccg atatctccac gggtcatagt tagacggaac tcttttgcag agaaaccacg
    13441 ggctgagtaa cgttgggaaa gattatacaa aaatgcagct agtttttctt ccgcattcat
    13501 ttttgagagc aatagaatca tttcttgatc gcttttaatt tcattactca tcaaacgcat
    13561 aatttgatgg cgaatttttg gcattttgcc ggcaagatca tctaaaatat cgaatggaat
    13621 ctcacaaatc attgaggttt cgagtgcttg agcatagcct tcgtgctgca tattggtaat
    13681 tgcatcaaag ccaattagat cgccaggtaa atggaaaccg gtaatttgct cttcaccact
    13741 ttcacttaag gtataggctt ttaatgttcc agaacggata gcataaagcg aacgcagttc
    13801 atcacctaac ttaaaaataa cttgcgattt ttgaatcggt tttttacgct caataatatt
    13861 atctagttga gttaattccg tttcattaag agtaaaaggt aagcatagtt ggctaatact
    13921 gcaattttga caatgaattg tgcaagtatg acgtccgctt gttttaggtt ctgatacaat
    13981 tttcatccat tcaaccttaa attagaaaag tattcctaaa tttgatttag aacaaagttt
    14041 accattaaat ttacatgaag caaagtatac caaggaaaaa taaaaatact tacaaagagg
    14101 tatgaaaaat ataatcaaat ttttattttt gagatctact tcaagttttt atcaaaaaga
    14161 gtgtgttagt atgtttatac acaaagtaaa caactctcac aagcaaggag catattatgt
    14221 ataaacatat cttagttgca gtggatcttt ctgaagaaag tcttgtttta gtgcgtaagg
    14281 gggctgcgtt agcagaaaaa tgcggagcta aactttccct cattcacgtt gatgttaatt
    14341 tttcggatct ctatacagga ttaattgata ttaatatgtc ttcggtacaa tacagtgttg
    14401 ttgaagaaac aaatacggca ctggctgaac ttgcctcaaa agtaaattat cctgtatctg
    14461 aatgtttaaa cggaacggga gattttagtc aagttttgga agaggccgta gaaaaacaca
    14521 atgtcgattt actcatcact ggccatcatc aggacttctg gagtaaattt atgtcttcaa
    14581 ctcgtcgagt catgaataat atcactgttg atatgctggt tgtgccatta gcagatgaat
    14641 agcaagaaag tgaatgaaaa ataaccgctt gagacagttt atctgtctta caagcggtta
    14701 tttttttggg aaaa
//
LOCUS       DANLXQ010000054        14220 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.054, whole genome shotgun sequence.
ACCESSION   DANLXQ010000054 DANLXQ010000000
VERSION     DANLXQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14220
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.054"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..633)
                     /locus_tag="PXD94_001237"
     CDS             complement(<1..633)
                     /locus_tag="PXD94_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL6286109.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSPAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAVFAKSAKVRSTLNQRDKYSNDLPYIRFVKSIQTCQC
                     RDGKYCHTEKVLVENKNGAVQLCWHHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSTAIEFADLVLFASIKGLLDELDSKALQQFLGYPPLNNSGVSKES"
     gene            complement(760..1353)
                     /locus_tag="PXD94_001238"
     CDS             complement(760..1353)
                     /locus_tag="PXD94_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL6286110.1"
                     /translation="MTDFDKNTYQTPNYVRNWLNHRYAWFHIDGCSNGQNTLYTYWIG
                     RAADGLDDDKLSCQIADDFLADDLFDVLLDKVSDWGELLRIFVNPPYSDPLPFVQRAA
                     ELKKAGHLVVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRIQFINPVTG
                     KPAQGNNKGSMIVVFDPFIEGIVTRQMPLDKIKEWGK"
     gene            complement(1364..2410)
                     /locus_tag="PXD94_001239"
     CDS             complement(1364..2410)
                     /locus_tag="PXD94_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL6286111.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNPSIGNNSEQTRKNFRESSEKNPTDNNTNYKNNNDHTTPLNPPAGEPAP
                     AEVVLNYLNSALVTLAVQLGERKPVGYSLKPWAKNITARIRESSVADCCQVVDYLVAK
                     WGRDEKMREYLCPKTIFRQSNFADYFPKSTAWANNGKPVCVNGKWVTPAELEKRLIMP
                     TVDEVRALFQKSLSGNPFKALDFTNKRNLVMYHATINTRNKRPLERDLPMIISQEIKN
                     AVERIDRLRVPTFS"
     gene            complement(2407..3090)
                     /locus_tag="PXD94_001240"
     CDS             complement(2407..3090)
                     /locus_tag="PXD94_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDL6286112.1"
                     /translation="MNQLLNISAPKASITMSSREIAELCEKEHRHVTRDIEAMFSQLD
                     IPAEGYAHFWTHPQNGQTYREFRLPYDLTLTLISGYSVILRKKIIDRWQELESRQGIP
                     QSFSQALRLAAEQQEVIEQQNHQLAIQAPKVAFVEHYVEVGTTKSLRETAKILNFPEK
                     RLIECLERYRVLYRQSGNLLPYQDKQAKQLFAVKTGTAEHGHNFTQTRVTAKGIEWIA
                     QRYASELGQ"
     gene            complement(3148..3336)
                     /locus_tag="PXD94_001241"
     CDS             complement(3148..3336)
                     /locus_tag="PXD94_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL6286113.1"
                     /translation="MKKDDVLEHFGTLEKVAATLGISVSAVSQWGEIIPEKNAYRLQE
                     ITAGKLKVKHSLYRNRSK"
     gene            3461..4117
                     /locus_tag="PXD94_001242"
     CDS             3461..4117
                     /locus_tag="PXD94_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6286114.1"
                     /translation="MNTQTLGDRIKSRRKELKLTQKDLATALKGVSHVAVSQWESSTT
                     KPNSENILDLSTVLQCDISWLLRGNGESNVLPASLGMTKVPLISYIQAGQWTGIDDFR
                     ETCGDYEYILTDLEVSEDAFALEIKGDSMEPDFIEGDRVIIDPDVQPHAGEFVAAING
                     DYEATFKKYRPLEDLDEYGRQHFELIALNPDYHKLSTLKQEIRIIGTMVEHRIYRRKR
                     "
     gene            4129..4503
                     /locus_tag="PXD94_001243"
     CDS             4129..4503
                     /locus_tag="PXD94_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286115.1"
                     /translation="MTEFKDVKQAIVEAQLASTPKDKVVNDIMKGSAELLSANDIINR
                     LGIGADEFQELVNLPNHYLRINGGLGAAVYNTRRMFDTHVELVNQELEKNISFPKPDL
                     YILGKARWKKETFKKWLEEQCR"
     gene            4655..5392
                     /locus_tag="PXD94_001244"
     CDS             4655..5392
                     /locus_tag="PXD94_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL6286116.1"
                     /translation="MLSKQVLSISESRLSTYLFCFFRNDPTKQKEAIAIYTALQHRTG
                     IYFSLIQEIEVALRNEVSQLLRFAAPNHNLYEFFHFLANDSNAPLNAESKRQLQKAIS
                     ECGRNYNENDIISHISFGFWVNLFNYDIKRNQYVAYWQSVLKPIFGHRFSNFKDLYNT
                     LKQIMRFRNRLYHQEVVWNKRIAKKPTHALENLEKTYKQFELALQKIAPERFAFRQLS
                     QALIWQRQIFFDQQIFEAEIVILPQHI"
     gene            complement(5428..6024)
                     /locus_tag="PXD94_001245"
     CDS             complement(5428..6024)
                     /locus_tag="PXD94_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286117.1"
                     /translation="MKKLLMTLCCLPAFALANETGESCSKIEDSAKRLECYDSVFVKK
                     DTGKDEATDEKSNWEYEQKKDELRNATTYFAKIRSTNTIDFGFPYNSSSMNLMLRKDP
                     KYGNDVIFSVHGQFNGCMIESCKITVKFDDGKLESYRMIGADGGSNDTLFIENAKAMK
                     TFVDKLKKSKKLIVEASFYNYGKGQFTFDTQGLEWKHF"
     gene            6670..6855
                     /locus_tag="PXD94_001246"
     CDS             6670..6855
                     /locus_tag="PXD94_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286118.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGFWQ
                     MESNGNQYCGGKYGTKN"
     gene            6839..7036
                     /locus_tag="PXD94_001247"
     CDS             6839..7036
                     /locus_tag="PXD94_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286119.1"
                     /translation="MARKIDAGVEIVEVDKRFIAKFFYDGKLQHTTYPQYNRQNAIML
                     INRKIERFNIGRDKPIKLYKE"
     gene            7039..8013
                     /gene="ssb"
                     /locus_tag="PXD94_001248"
     CDS             7039..8013
                     /gene="ssb"
                     /locus_tag="PXD94_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6286120.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKTS
                     GEKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIRVRWITKEQA
                     KQFINNISTDWMKTICSFALLTGARRTEILSMTWDKIDFIRKVAIVSNDIAKSEKARS
                     LLLNDDAIELLQKQRGKNPKYVFVGSKGQALQDINRKIFNKATEKCLLVDFHFHDLRH
                     TWASWHVQAGTPLFTLKELGGWETLEMVKKYAHLNADHLLNHANSVKF"
     gene            complement(8100..8176)
                     /locus_tag="PXD94_001249"
     tRNA            complement(8100..8176)
                     /locus_tag="PXD94_001249"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8140..8142),aa:Arg,seq:tct)
     gene            complement(8201..8277)
                     /locus_tag="PXD94_001250"
     tRNA            complement(8201..8277)
                     /locus_tag="PXD94_001250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8241..8243),aa:Pro,seq:tgg)
     gene            8447..9307
                     /gene="folD"
                     /locus_tag="PXD94_001251"
     CDS             8447..9307
                     /gene="folD"
                     /locus_tag="PXD94_001251"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL6286121.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYLEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIASKSYDLPEETTEAELLALIEKLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QTYENHLQAV"
     gene            9421..10872
                     /gene="thiI"
                     /locus_tag="PXD94_001252"
     CDS             9421..10872
                     /gene="thiI"
                     /locus_tag="PXD94_001252"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL6286122.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVDEETALVLDLLKRTPGIHHILEVEETPFTDMHDIFEQTYAAVKAEL
                     EGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTNPDVTVRIDIEHDKML
                     LIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEI
                     GVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMGVVLKRMMVRAASRIA
                     ERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHDKEQIIAMAKAIGTD
                     DIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQTASYLDIREIAQQTE
                     KEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELPFYKLSSQFSTLDQSK
                     TYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
     gene            complement(10917..11624)
                     /locus_tag="PXD94_001253"
     CDS             complement(10917..11624)
                     /locus_tag="PXD94_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL6286123.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(11692..12666)
                     /gene="pfkA"
                     /locus_tag="PXD94_001254"
     CDS             complement(11692..12666)
                     /gene="pfkA"
                     /locus_tag="PXD94_001254"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL6286124.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
     gene            complement(12773..13969)
                     /locus_tag="PXD94_001255"
     CDS             complement(12773..13969)
                     /locus_tag="PXD94_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL6286125.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVALNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQSKIAIRQGEHCAKPYLRHLEEVGTVRLSLAHYNNQQDVEHFFNALDFTL
                     EILQES"
BASE COUNT         4340 a         2804 c         2871 g         4205 t
ORIGIN      
        1 actttctttg ctcacaccgc tattattcag tggtggatag ccgagaaact gctgtaatgc
       61 cttgctgtct aattcatcaa gcaaaccttt gatactcgca aacaacacta agtccgcaaa
      121 ttcaatagcg gtagattgcg ttttgcgtaa ggttaagcgg atttgttggg cgataaattt
      181 ttcccagttt tcatccgcta gtgcttctaa tttttcattc gggatttcac cgtccattcg
      241 cattttgtcg tgatgccaac aaagctgtac cgcaccgttt ttattctcta ccagcacttt
      301 ttccgtgtgg caatatttgc catctcggca ttggcacgtc tgaattgact ttacaaaccg
      361 gatataaggc aaatcattac tatatttgtc acgctgattt agcgttgaac gaacttttgc
      421 cgatttagca aacaccgcca gctctttaca agcggtcgaa tttgccgaaa aatttgcaac
      481 cttgcctgat ttaaccgtcg ctaaatcggt cggtgcaggt tgcaacaaag ttcgctcacc
      541 gaaagcagca ggagaaacat ctttaggcac tttgtaaaac acaataccca cttcggtttg
      601 aaagtaaggg gtaagtagca aaccttccgc cattattgcc cccatttctc ggtttcatca
      661 taggctttca cgaatagcat aaatacgcca atacagatta aaaaaatcag tacgccaatt
      721 actgcaccaa taccaataaa tagttcccaa taatccattt tatttacccc attccttgat
      781 tttatctagt ggcatctgac gagttacaat gccctcaata aatgggtcaa acacaacaat
      841 catcgagcct ttattattac cttgtgccgg tttacctgtt accgggttga taaattgaat
      901 ccgccctgtc cgccaagtgc ctttttcatc gtgatagcca ataatatcaa ttacctcatt
      961 agcgtgttgt tgaatgatgg tgtaccattc tgttgtttta tccgctggta ataacatcac
     1021 aaccaaatga cctgcttttt tcaattctgc cgctcgttga acaaaaggca gtggatcaga
     1081 atatggcgga ttaacgaaaa tacgcagtaa ttctccccag tctgaaactt tatctagtag
     1141 cacatcaaat aaatcatcgg ctaaaaaatc atcggcaatt tggcaactta atttatcatc
     1201 atctaaaccg tctgccgctc taccaatcca ataggtgtac aacgtatttt gtccgttaga
     1261 gcaaccgtca atatgaaacc aagcataacg atgatttagc caattacgca cataattagg
     1321 tgtttggtaa gtgtttttat caaaatctgt cattgtctaa tccttacgaa aatgttggta
     1381 ctctcaaacg gtcgatacgc tcaaccgcat ttttaatttc ttggctgata atcatcggca
     1441 aatcacgttc aagtggtcgc ttgtttctgg tgttgattgt ggcgtggtac atcactaaat
     1501 ttcgtttgtt ggtaaagtcc aatgccttaa acggattgcc actgagtgat ttctggaaca
     1561 aggctcgtac ttcgtccacc gttggcataa tcaagcgttt ttccagctcc gccggtgtaa
     1621 cccacttacc atttacgcaa acaggctttc cgttattagc ccaagcggtc gattttggaa
     1681 aataatctgc aaaattcgat tggcggaaaa tcgttttcgg gcagaggtat tcacgcattt
     1741 tttcatctcg tccccattta gcgacaaggt aatccaccac ttggcaacag tccgccaccg
     1801 aactttcacg aatgcgagca gtaatatttt ttgcccacgg cttgagcgaa taccccacag
     1861 gcttacgctc accaagctgt accgccaacg ttaccaaagc cgaattgaga taattcaaca
     1921 caacttcagc cggggcaggt tcccccgctg ggggattaag gggggtagta tgatcattat
     1981 tatttttata attagtatta ttatctgtcg gatttttttc cgagctttct cggaaatttt
     2041 tccgagtttg ttcggagtta tttccgatac tcggattttt ttccgactca cggatcggct
     2101 cgaatttatt ccattcacaa cctttttcgg ttaaacggac ataatcttta ccgtccattt
     2161 tgaagtggtc gataatgcct ttttctttca acaccttata ttggcggtaa actgtatctt
     2221 ctttgctaaa aaccgcaggt aactcttcgc aaaccttgct aaatgacatc cagtaatagg
     2281 ttatgccgtc aataaccacc gctttcgccc aactggaggc ttgattgatt aaatcgacca
     2341 atgccccttg tgtaatattg atttcccatt ccactaagcg gacattgttt atggttgatg
     2401 taaatctcat tgtcctaact ccgatgcgta acgttgagca atccattcaa tccctttggc
     2461 ggttactctc gtttgggtaa aattatgacc gtgttctgcc gtgccggttt ttactgcaaa
     2521 aagctgttta gcttgtttat cttgataagg gagtaggttt cctgattggc gataaagaac
     2581 tcgatatcgt tctaaacatt caatcaaccg cttttctgga aaattgagta tcttggcggt
     2641 ttctcgcaat gatttagtcg ttcccacctc aacataatgc tccacaaatg cgactttcgg
     2701 ggcttgaatg gctaattgat gattctgctg ctcaatcact tcttgctgtt ctgccgccaa
     2761 ccgtagagct tgagagaaag attgtggaat accttgtctg ctttctagct cttgccaacg
     2821 gtcgataatt ttcttgcgaa gtatcacgct atagccggaa attagggtaa gggttaaatc
     2881 gtaaggcaat cgaaattccc ggtatgtttg cccattttga gggtgtgtcc aaaaatgggc
     2941 atacccctcg gcaggaatat ctaattgact aaacatcgct tctatatcac gagtaacgtg
     3001 tcgatgttct ttttcgcaca actcagcaat ctcacgacta ctcatcgtaa tgcttgcttt
     3061 tggtgccgaa atgtttaata attgattcat cgaaaatacc tttcgtgttt aattatgtat
     3121 gaaagccacc gttacagcgg tggtttttta tttgcttcta tttctataaa gtgagtgttt
     3181 gacctttaac ttgccggcag tgatctcttg taatctgtac gcatttttct caggaataat
     3241 ctcgccccat tgagaaacag cagatacact aatccctaaa gttgccgcca ctttttctag
     3301 agttccgaaa tgttcgagca catcatcttt tttcatattt tcttaattcc tttcttgaat
     3361 tttatttaag acctcttaaa tatacaatta aaggaaactt aagtcaataa atgttaagat
     3421 aacttaacag caactttatt tttaccttta aggggatact atgaatactc agaccttggg
     3481 cgatagaatt aaaagccgta gaaaagaatt aaaattgaca cagaaagatc ttgctactgc
     3541 gttaaaaggg gtctcacacg ttgcagtatc tcaatgggaa agtagtacga ctaagccaaa
     3601 ttcagagaat attttggatt tatctacagt attgcaatgc gatattagtt ggctcttgcg
     3661 tggtaatgga gagtctaatg ttctacctgc tagcctcggt atgactaaag tcccattaat
     3721 tagctatatt caagccggac aatggacggg aattgacgat tttcgtgaaa cctgtgggga
     3781 ttatgaatat atcctaactg atcttgaggt gtctgaagat gcttttgccc tagaaatcaa
     3841 aggggactcg atggagcctg attttattga aggagaccga gtaattattg atcctgatgt
     3901 acaacctcac gccggtgagt ttgtggcagc aattaatggc gattatgaag ccactttcaa
     3961 aaaataccgt ccgttagaag acttagacga atacggaaga caacattttg agcttatcgc
     4021 tcttaatccg gattaccata aattatcaac gctaaagcaa gaaatcagaa ttatcggcac
     4081 aatggtagag catcgtattt atcgtagaaa gagataggaa ttgataatat gacagaattt
     4141 aaagatgtaa aacaggcgat tgtagaagct caattagctt cgacacctaa agataaagtc
     4201 gttaatgaca taatgaaagg aagtgcggaa ctgctttcag caaatgatat tatcaatagg
     4261 ttaggtattg gggcagatga gtttcaagag ttggttaatt tacctaacca ttatttgcgt
     4321 ataaatggag ggttaggtgc tgctgtgtat aatacaagaa gaatgtttga tacccatgtt
     4381 gagttagtca atcaagagtt agaaaagaat atttcatttc ctaaacctga tttatatatt
     4441 ttggggaaag ctcgttggaa aaaagaaaca tttaagaaat ggttggaaga acagtgtaga
     4501 taaagaaaag gtaggttaaa aaacctacct tttctgaacc gcaaataagc gattctcaca
     4561 ctttacaaag caatccagct cctacgtctg gggcttcggg tccgttgcca gtgcaagtat
     4621 tattacatta aactaaactt gagtaaagga aaatatgttg tccaaacaag tgctaagtat
     4681 ttcagaaagt cggctgagta catatttatt ttgctttttc cgaaatgatc ccacaaaaca
     4741 gaaagaagct attgcaattt ataccgcctt gcaacatcga acaggaattt atttttcatt
     4801 gattcaagaa atagaggtgg cactaagaaa tgaagtcagc cagttgttac gctttgctgc
     4861 acctaatcat aatttatacg aattctttca ttttctagcg aacgatagta atgctcctct
     4921 taatgcagaa tctaaacgcc agctacaaaa agccattagt gaatgcggac gtaactataa
     4981 tgaaaatgac ataatttctc atatttcttt tggattttgg gtaaatctgt ttaattatga
     5041 tataaagcgt aatcaatatg ttgcttactg gcaaagcgta ttaaagccta tttttggaca
     5101 tagatttagt aattttaagg atttatacaa tacattaaag caaattatgc gttttagaaa
     5161 tcggctgtat catcaagaag tggtttggaa taagcgaatt gccaagaaac caacgcacgc
     5221 actagagaat ttagaaaaaa cttacaagca atttgagtta gcactacaaa agatagcacc
     5281 tgaacgattt gcgtttcgtc agctttcaca agcacttatt tggcaacgcc aaatcttttt
     5341 cgaccaacaa atttttgaag ctgaaatcgt tattttgccc caacatatct aagcaaaaag
     5401 ctacgcatct atgtagcttt ttcttaatta aaaatgtttc cactctaacc cttgtgtatc
     5461 aaaggtaaat tgccctttgc cataattgta aaaactcgcc tctacaatta gttttttcga
     5521 ctttttcaac ttatctacaa aggttttcat tgcttttgca ttttcaatga aaagcgtatc
     5581 attgctgcca ccatccgccc caatcattcg ataactttcc aatttaccat catcaaactt
     5641 cacagtaatt ttacagctct caatcataca gccattaaat tgaccgtgta cgctaaaaat
     5701 tacatcattg ccatatttag ggtctttgcg taacattaaa ttcattgatg atgaattata
     5761 cgggaaacca aaatctattg tgttagttga tctaatttta gcaaagtaag tcgttgcatt
     5821 acgcaattca tctttctttt gttcatattc ccaattagac ttttcatcgg tagcttcatc
     5881 tttaccagta tcttttttca caaagacact atcataacat tctaagcgtt ttgcactatc
     5941 ttcaatctta gagcaacttt ctccagtttc gttagctaaa gcaaaagcgg gcaaacaaca
     6001 taaagtcatc agtagttttt tcatataacc tccatagaaa tcccttctta ttttctcaaa
     6061 agcgtggttt tcggtctgtg atcaagctcg caatctcaat aattatattt taatggttta
     6121 aaaaacaatc aattaagaaa tcataactaa ttgaaattta agaaatctta aaaatactta
     6181 ttaaataagc ttaaattttg attgacttgt aatttaagtt ttcttaaact taattcatca
     6241 aaacgagcta cggctcaatg ctctttaaca attcggtgct tgtgcggacg atattaacaa
     6301 cctcgggcag ttgtaaagta atgctttata actcagtaag gttggttaga ccctgacata
     6361 caaatttgaa cgacttgcca aaaagaggtg gcaaggttta ggcaacacac tcgcaaggtg
     6421 tggagagctg aaaaggcaac actggtatca gtcctaagca atcacgctta tctcaagttt
     6481 gagtaagtac ggatacaagc agcacccgac ggaatggcga aagccgggcg ggcgacggcg
     6541 tgggcagaca cgcatttatt ccaaagctca tttcaccaag tgtgtttcgg aatacccaca
     6601 actagacgca agtcgctcca tttgcccacc gtaaaacgtg ggcttttttt aaccctaaag
     6661 gacaatccta tgaacaaaat gaaacaactt gccccatttg ccttgctaat tttgctatta
     6721 ggcattgtcg ggcgaatgga ttatgacgac cacatacaaa tgcaacgtta caaatgcgaa
     6781 cgaaatcaag gcttttggca aatggaaagc aacggcaacc aatactgcgg agggaaatat
     6841 ggcacgaaaa attgatgccg gtgttgagat tgttgaggta gataaacgat ttatcgccaa
     6901 gtttttttat gacggcaaat tacagcacac aacctatcca caatacaacc gccaaaacgc
     6961 aattatgttg attaaccgca aaattgagcg gttcaacatt ggcagagaca aaccaatcaa
     7021 actttataag gaataacaat ggcaggaatt aacaaagtga ttattgtcgg tcgcctaggt
     7081 aacgacccgg aactacgaac aatgccaaat ggcgattcag tcgccaaaat cagtgtagca
     7141 acttcaaccg aatggacgga taaaacctca ggcgagaaaa aacaagccac agagtggcat
     7201 agcattatcg cctttcggaa tttagctgac attatcggca aatacctgaa aaaaggctcg
     7261 caagtgtatg tggaaggcaa gctacgcact cgcaaatggc aagcccaaga cggcacagac
     7321 cgctggacga ccgaaatcat cgccgatcag ctacaaatgc tcggtagctc aaacagcgga
     7381 aacagcaata ataattgggc aaccgaaccg gcaggaaacc caccaccaac caatccatat
     7441 aaccacgtaa tgacggattc agaatcacga gattttgatg atgatatccg ggttcgttgg
     7501 attaccaaag aacaagccaa gcagtttatt aataatatta gtactgattg gatgaaaact
     7561 atctgctctt ttgctttatt aacaggagca aggcgaacag aaatactttc tatgacttgg
     7621 gataaaatag attttatccg taaagtagcg attgtttcga atgatattgc caaatctgaa
     7681 aaagcgagat cgcttttact taatgatgat gcaattgaat tattacaaaa gcagagaggg
     7741 aaaaatccta aatatgtttt tgtaggaagc aaagggcagg cattacaaga tattaaccgc
     7801 aaaatattta acaaagcgac tgaaaaatgt ttattggttg atttccattt ccatgattta
     7861 aggcatactt gggccagttg gcacgttcag gccggcacac cattatttac cttaaaagaa
     7921 ttgggtggct gggaaacatt agagatggta aagaaatatg cccatcttaa tgcagaccat
     7981 ttactcaacc acgctaactc agttaaattc tagtcacaaa ttagtcacaa agtatttttg
     8041 acataggaaa ttaataactg atatttaaat gtaactattt gaaatataag ggtaattaat
     8101 ggcacgccct aaaggattcg aacctttgac ccacgcctta gaagggcgtt gctctatcca
     8161 gctgagctaa gggcgcagat gaagataata taaaaagaag tggtcggcga gataggattt
     8221 gaacctacga cccactggtc ccaaaccagt tgcgctacca agctgcgcta ctcgccgaca
     8281 taagatgttg ttgattatag atttctttgc acaacagtca atcttttttt gtgaatgtcg
     8341 gcttaaatga ttgaagttta tccgttattc aaaattagct tatcaaaaag gctatttttt
     8401 gagagaatga cgaatgtttt gccaagtgat atttcgggag gtagtaatga ctgcacaaat
     8461 tatttcgggg agtgaggttt cttcacaaat taaaaaaaag gtgagtgaga aaatcgctca
     8521 ttatcttgaa cagggtaaac gtaaccctgg tcttgcggtc attttagtgg ggaatgaccc
     8581 agcctctcaa gtgtatgtgg ggagtaaacg caaaagctgt gccgaaattg ggattgcgtc
     8641 gaaatcttat gatttacccg aagaaaccac agaggctgaa ttattagcac tgattgaaaa
     8701 gttgaatcaa gatgattcgg tcgatgggat tttagtccaa ttaccgttac ctaaacagat
     8761 cgattcgact aaagtgattg aagccatctt accacataaa gatgttgatg gattccaccc
     8821 ttataatgtt gggcgtttat ctcagcgtat tcccacttta cgcccttgca cgccatacgg
     8881 tgtgatgaag ttgcttgaaa caactggggt agatttatat ggtcaacacg cagtgattgt
     8941 aggtgcgtct aatattgttg gtcgcccgat ggcgttagaa ttgttgcttg gtggttgtac
     9001 agtcacagtt actcatcgct ttaccaaaga tttagagcat cacgttcgcc aagcagatat
     9061 tttagttgtg gcagtcggca agcctgaatt tattcccggg gagtgggtta aagacggtgc
     9121 tatcgtgatt gatgtaggga ttaaccgtgg agcagacggc aaactaaaag gtgatgtaga
     9181 gtattcagtt gcctcacaaa aagcggcatt tatcacccct gtccccggtg gggtaggccc
     9241 gatgacggtg gcaatgttaa tgcaaaatac cttgcagacg tatgaaaacc atttacaagc
     9301 ggtctaattt tacggatatt ttgcaaatcc tcggcattgg tcgaggattt ctttttctgg
     9361 gctttttcag cagaaaaggc taaaatactt gccattttta ttacacgatc cttattaaat
     9421 atgaaattta tcatcaaact tttccctgaa attatgatta aaagcgactc tgttcggaaa
     9481 cgctttatca aaattttaac ctcaaatatt cgcaacgtat tattaaaaga gactgaaaat
     9541 gtgtctgtgg ttcgccattg ggactttatt gaagttcggg cgaaagtgga cgaagaaacg
     9601 gcattagtgc tggatttgct taaacgcacg ccgggtattc accatatttt agaagtggag
     9661 gaaacccctt ttacggatat gcacgatatt tttgagcaga cttatgccgc agtgaaagct
     9721 gaactagaag gcaaaacgtt tagcgtgcgg gttcgccgta aaggaaagca cgatttccgt
     9781 tctcttgatg tagagcgtta tgtcggtggt gggctgaatc agcggattga aacggcgaaa
     9841 gtgaaattaa ccaacccaga tgttacggtc agaattgata ttgaacatga taaaatgttg
     9901 ctgattaaag ctcgccacga agggttaggc gggtatccaa tcggcacaca agaagatgtg
     9961 ttgtcattga tttcgggtgg ttttgattcg ggtgtatcga gctatatgct gattcgcaga
    10021 ggttctcgtg tgcattactg ttttttcaat ttaggtggtg ccgcacacga aattggcgtg
    10081 aaacagatgg catatcatat ttggagccgt tatagtacct cgcacaaagt ccgttttatc
    10141 gccattaatt ttgaaaatgt agtgggtgag attttagaaa aagttgataa cggacaaatg
    10201 ggcgtggtat taaaacgtat gatggtgcga gcggcaagcc gtattgccga gcgttttgat
    10261 attcaagcga ttgtgaccgg tgaggcgttg gggcaggtct ctagccaaac actgactaat
    10321 ttacgtttaa ttgataaggc atcagatacg ctggtattac gtccgctcat cactcacgat
    10381 aaagagcaga ttattgcaat ggcaaaagcg ataggcactg acgatattgc taaatcaatg
    10441 ccggagttct gtggagtaat ttctaaaaat ccgacggtta aggcaatcga aactaaaatt
    10501 ttggaagaag aaggcaactt taattttgag gtactagaac aagcggttca aacggcgagt
    10561 tatttagata ttcgtgagat cgctcagcaa accgaaaaag aggttgtttc ggttgaaacc
    10621 acaacagaat tgagtaaaaa cgatgttgtg ctagatattc gcagcccgga agaggttgat
    10681 gaaaaaccat tccgtttaga cggggttgaa gtaaaagaac tcccgtttta taaactttcc
    10741 agccagttta gtacgcttga ccaaagcaaa acctacttgc tctattgcca acgtggggta
    10801 atgagtaaac tgcaggctct ttatttgaaa gagaatggct acagcaatgt aaaagtgttt
    10861 cgagtgaaat aatgcctctt tgattgcacc ttaatgattg attaaggtgc aattttttac
    10921 caaataaggc ttgctaaaaa gagtaagatc acaggaaatt caagcattcc aacttgttta
    10981 acattccagc caaagcttgg cacaataatg gctcgggcag ttgcaattaa ataagcaaaa
    11041 aagaccgctt gtagagagca gagtatgtaa agcattgcta aaaccaaatg tattgcaata
    11101 ctgatttcca tataaattgg gtttcttcgc tctcgcacca tactctttat atagaaagtc
    11161 gtagcaataa aaaatagggt agggtggagc agaatctcaa actgatattg tttcgttacc
    11221 agataataag ctgccattcc cattatgcca aaggttagaa tgccggcaat atcattccat
    11281 aaattgcgtt catctttttg tttggcgtag tgaacttgca ctaacccaag cggtagaatt
    11341 atcggtaaaa attgcaaaat cgaaggctga gcgtagatca aaggtaatgc aaatagaatg
    11401 cttaagccaa agtagatttt cgcccatttt ttattgcgtt cagttggctt tttactgaaa
    11461 agtgagagaa aagggtagga aaagaagtag agaaacagcc acgacagccc aaagaaaata
    11521 tgcaaagtgc taaaaccact ttcaataacg gcgtagagaa acggcaacat tgccattgct
    11581 aatgcaccgt gttggttgga tataacgggt ttttctttga acattcaatt tccctcttaa
    11641 tgtggcaaat aaaaaggcga ctaaacagcc gcctttaaat tgtaagaaaa gttagaataa
    11701 ttttctagag gcttcaaata attcttcttt gaacggacga cgcatattat tgatcgcatc
    11761 aataatatcg tggtgaacca actgctcgtt ttgaataccg acacagtgac cgccaaagcc
    11821 ttgcagtaat aattccaccg catagacgcc catacgggag gccaaaatac ggtcgaaggc
    11881 acaaggcgaa ccaccacgtt gaatatgacc taatacggtt gcacgggttt cattgccgaa
    11941 acgtgcttca atttctttcg ctaatgcgtg aacatcggtc atcaattctg taattgcaat
    12001 aattgaatgg cgtttacctt tgtggaaacc ttcttcaatg cttcgcatta atgcatcttt
    12061 atctaagcct ttttccggta cgatcacata ttcgcaacca ccggcgaggg cagcacttag
    12121 ggttaaatca ccacagtggc gacccataat ttccacaatc gaaatacgtt ggtgtgaact
    12181 tgaagtatca cgcaggcggt caatcgcatc taatgcggtt tcaagggcag tttgatagcc
    12241 gatggtgtaa tccgttccca caatatcgtt atcaatcgta cccggaatac cgatacaagg
    12301 gtaaccaaat tcttcggtta ataatttagc ccccatataa gaaccgtcgc caccaataac
    12361 cactaatgca tcaatgctgt atttttttaa ggtttcgaca caatgtttgc gaacttctgg
    12421 tcttttaaaa tcagggaagc gggcagaacc taagaatgta ccgcctcggt tgatggtttc
    12481 agacacggaa cggcggtcaa gtgggatcac tttatcgtgg taaagcccat agtagccgtc
    12541 ttgaatacca caaacctcta aaccttcatt aagtgcggta cgaaccacac cacggattgc
    12601 cgcgttcatt cctggtgcat caccaccact ggttaatact gcaatttttt tgattttttt
    12661 actcatttaa aaacaccttg ttgataaatt aaagttaaat tggaaactgg tgcaaggtta
    12721 caatattcag ccttttcgtt ctactaaaaa atacgtattt ttttgattta gcttagcttt
    12781 cttgcaaaat ttcaagggta aaatctagag cgttgaaaaa atgctcaaca tcttgttgat
    12841 tattgtaatg ggcaagtgaa agtcgaactg ttccgacctc ttctaaatgg cgtaaataag
    12901 gcttggcaca atgctcccct tggcggatcg caattttact ttgggttaag aaagcggcaa
    12961 tatcggcagg gtgctgattt ttcaccacaa agctaatagt cggtgtattt tgttgcccga
    13021 taatttgcaa attttgataa gaatttaacc gcttgcaggt gtattcagcc aagtcataaa
    13081 gctgatgatt aagagcgaca aaatcccatt tttcgagcca agctagcact tgcccaaagc
    13141 cgataatgcc ggcgatattc ggcgtgccag cttccaagcg ataaggtaac tctgccacag
    13201 ttaattcact ttccgtaatg tttttcagca ttttgccgcc aaatacaagc ggttgaattt
    13261 gctccaaact ttgcaatttg ccggtaagca cgcccacacc gtttggacca tacattttat
    13321 gggcggaaaa agcgtagaaa tctgcatcta atttctgaac gtccacttgc tctgagcaaa
    13381 ctgcttgagc aatatctaac acaattttag cagagcaaga ttggcggata agcgggatta
    13441 tttgttccgc tggctggcga actcctgtta cgttagaaac taaattaaag gcaacaattt
    13501 tggttttttc cgataacgca tttttaaggg tttcaagctc aatttcatac ttatcattaa
    13561 gtggcaacac cacgagtttc gcccttgtac gctgtgccaa ctgctgccaa ggaataaaat
    13621 tagcgtgatg ttccgccact gaaattacaa tttcatcgcc tgctgcaatt tgatgctcca
    13681 agccataagc cacggtgtta atactttggg ttgtaccgct tgtccaaatc accgcttcac
    13741 gagtttccac attgaaacgt tttgcgacta aatctcgagc taattcatat tgttcggttt
    13801 gtggcaaatc gtactgactg cgatgcaccg accccgcaga agcgtaaaac tcacttgttg
    13861 cttcaatgag tacatcaggt ttcagcgttg tagcggcaga atctaaatac gtccatccgt
    13921 cagatttttc aaaaaaaggg aaatgggtgc gaaattgggt gttaagcata agttaattcc
    13981 taagagttgt ggaaaattga gttaccaaga aaatacctat tataaaggaa tgttttgagg
    14041 taagaagcat ttcaagaaat aaatagattt attttgttat ctttttgtta aattcgtgtt
    14101 gactaataaa attattttta taaaatccac ataaaaaata tggtctattt aggggggggg
    14161 taaaaaataa atattactaa tgtatgagaa gaagataatc tatatttttt aaaatgccct
//
LOCUS       DANLXQ010000055        14096 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.055, whole genome shotgun sequence.
ACCESSION   DANLXQ010000055 DANLXQ010000000
VERSION     DANLXQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14096
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.055"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(108..800)
                     /locus_tag="PXD94_001256"
     CDS             complement(108..800)
                     /locus_tag="PXD94_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL6286126.1"
                     /translation="MTELTPKQEAFCFAYIETGNASEAYRQAYETEDMKSETVHRKAK
                     ELMDNGKITARIEELKAEHAERHKLTVDDLLKELEEARILAREKENPNAMTQATMGKA
                     KLLGLDKQVTDPCFDGETGKGDMILSVNCSSIKDGKALDETCILKAGTHEFITRDSFI
                     FYRHIRIDSLDEIKANIEAGYFIQKALINDEAYQQILKGVLSSKSVTPRYKRFLKNAI
                     RQSACPDILAEP"
     gene            complement(797..1222)
                     /locus_tag="PXD94_001257"
     CDS             complement(797..1222)
                     /locus_tag="PXD94_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286127.1"
                     /translation="MKHELNLAGITALMNEPIKATLSFLDFPHIRIERLHHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAIIAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNEITREQMSKEYREKIDYAFAEKRMAEGKE"
     gene            complement(1229..1381)
                     /locus_tag="PXD94_001258"
     CDS             complement(1229..1381)
                     /locus_tag="PXD94_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286128.1"
                     /translation="MPELLIVLGNRIAKLEQQHNAMPSETDSITLGILRKQLSQLLKV
                     AERIKE"
     gene            complement(1542..2228)
                     /locus_tag="PXD94_001259"
     CDS             complement(1542..2228)
                     /locus_tag="PXD94_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286129.1"
                     /translation="MADHSQFISRLENRIEPKISFQELIQELSNYTNKPFSHIADKLK
                     SLIFSFQQNGIYTNAFVNCTLFEDGESYAIEPKTLHFKDAFDQPKGVLSDVIAQNSID
                     VEHLNAYYVTAREITQLIKTQSPTLLDKLTTKQTQSEISQIQNDYISVYDFIEWASKH
                     YSSLSETANDLNRLLKDKNIQLYRQYGGISPSIDKDNTNLKAAFDFVAINNGYEKQSS
                     SFNFDDDIPF"
     gene            complement(2623..2946)
                     /locus_tag="PXD94_001260"
     CDS             complement(2623..2946)
                     /locus_tag="PXD94_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL6286130.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNDVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
     gene            complement(3059..3277)
                     /locus_tag="PXD94_001261"
     CDS             complement(3059..3277)
                     /locus_tag="PXD94_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module killer protein"
                     /protein_id="HDL6286131.1"
                     /translation="MMREQTTNQIIITDNFSKWLENLKNPIAKATVARRIKNAINGNF
                     GDHKSLAGTGGLYEMRIATGAGYRIYYA"
     gene            complement(3552..4778)
                     /locus_tag="PXD94_001262"
     CDS             complement(3552..4778)
                     /locus_tag="PXD94_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6286132.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFV
                     EECSQGSLPQYHLKIA"
     gene            complement(5053..5419)
                     /gene="ssrA"
                     /locus_tag="PXD94_001263"
     tmRNA           complement(5053..5419)
                     /gene="ssrA"
                     /locus_tag="PXD94_001263"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5585..6544
                     /gene="rpoH"
                     /locus_tag="PXD94_001264"
     CDS             5585..6544
                     /gene="rpoH"
                     /locus_tag="PXD94_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL6286133.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARG
                     YLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVK
                     VATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMTGQDLGFD
                     LPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDERSQDIIKT
                     RWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            6710..7003
                     /locus_tag="PXD94_001265"
     CDS             6710..7003
                     /locus_tag="PXD94_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL6286134.1"
                     /translation="MTQLSTSVYQRADGFINVANAHLKNIAPNQVSNAMLFGCARFNA
                     YVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD"
     gene            complement(7021..7323)
                     /locus_tag="PXD94_001266"
     CDS             complement(7021..7323)
                     /locus_tag="PXD94_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL6286135.1"
                     /translation="MNTYNNTLNNKNLEKKQLLMAIQAIPASQDFIWDGKDDDERPVT
                     PSEFSIAMKKRGRPAGSKKELVSLRLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
     gene            complement(7295..7585)
                     /locus_tag="PXD94_001267"
     CDS             complement(7295..7585)
                     /locus_tag="PXD94_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL6286136.1"
                     /translation="MKIYTWDETKRKINLAKHGLDFADADEVLSSPYRFDVEVIRNNE
                     FRIQSFAYSFGRLLVLSLAYTEREDYIRVISFRRASKAEREAYYEYLQQYFE"
     gene            complement(7723..8262)
                     /locus_tag="PXD94_001268"
     CDS             complement(7723..8262)
                     /locus_tag="PXD94_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL6286137.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFLTVAGKMAQ"
     gene            8333..9010
                     /locus_tag="PXD94_001269"
     CDS             8333..9010
                     /locus_tag="PXD94_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL6286138.1"
                     /translation="MSIAENLSQIHQQIQQISEQYQRDNVRLLAVSKTKPVEAIQAAI
                     EAGQRAFGENYVQEAVEKIEFFANRPELEWHFIGPLQSNKTKLVAAYFDWIQTVDRLK
                     IAERLNEQRPADKAPLNVLIQINISDEASKSGIEPDEMLPLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKVAFRKMQLLFNRLQTEFDDIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARR"
     gene            9195..11042
                     /gene="typA"
                     /locus_tag="PXD94_001270"
     CDS             9195..11042
                     /gene="typA"
                     /locus_tag="PXD94_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL6286139.1"
                     /translation="MQNVDISKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGETRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGASD
                     EQLDFPIIYASALNGVAGLEHEDLAADMTPLYEAIVKHVQPPQVELDAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTIVDSEGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETSNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(11091..11528)
                     /gene="rimI"
                     /locus_tag="PXD94_001271"
     CDS             complement(11091..11528)
                     /gene="rimI"
                     /locus_tag="PXD94_001271"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL6286140.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(11521..11943)
                     /locus_tag="PXD94_001272"
     CDS             complement(11521..11943)
                     /locus_tag="PXD94_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL6286141.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            12073..13062
                     /gene="rsmC"
                     /locus_tag="PXD94_001273"
     CDS             12073..13062
                     /gene="rsmC"
                     /locus_tag="PXD94_001273"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL6286142.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGNIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FDLPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
     misc_feature    13192..13314
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            13336..13713
                     /locus_tag="PXD94_001274"
     CDS             13336..13713
                     /locus_tag="PXD94_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDL6286143.1"
                     /translation="MKNPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVI
                     SRHLRNIYQEGELEREATVAKNATVQIEGAREVVRLKDYLVKGYALNERRLQERGIEF
                     EQVIGLLSQYTGCLSLVGGRKFA"
     gene            13780..>14096
                     /locus_tag="PXD94_001275"
     CDS             13780..>14096
                     /locus_tag="PXD94_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6286144.1"
                     /translation="MSINISPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVS
                     ESENVKGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEYRNQGIGKELIEY
                     IYR"
BASE COUNT         3974 a         2801 c         3000 g         4321 t
ORIGIN      
        1 gtgggtaact ttgattttta cccactgtta gccactttta gaggggaaat attagcggtt
       61 ttggtagtgg ttaaagggca aaaaaggcct taattctcat caacggctta aggttcggct
      121 agaatatcag ggcaagcact ttggcggatt gcatttttta ggaagcgttt atatctaggc
      181 gttactgatt ttgacgatag tacgcctttt agtatttgtt gataggcttc atcattaatt
      241 agggcttttt gaatgaaata accggcttct atatttgcct taatctcatc taacgaatcg
      301 attcttatat ggcggtagaa aataaagctg tcccgtgtga taaattcgtg tgttcctgcc
      361 tttaaaatac aagtttcatc aagtgctttc ccgtctttaa tgcttgagca gtttacactt
      421 aaaatcatat ctcctttacc ggtttcacca tcaaagcacg gatcggtaac ctgcttatcc
      481 agccctagca acttcgcttt gcccattgtg gcttgagtca ttgcgttagg gttttctttc
      541 tctctcgcca gtatacgagc ctcttccagc tcctttaaaa ggtcatcaac ggttaattta
      601 tggcgttctg cgtgttcggc tttcagttcc tcaatccttg ccgtaatctt gccgttatcc
      661 attagctctt tagcttttcg gtggacggtt tcggacttca tatcttcggt ttcataggct
      721 tggcggtagg cttcgctagc attgccggtt tcaatataag caaagcaaaa agcctcttgt
      781 tttggtgtga gttcggtcat tctttcccct ccgccattcg cttttcagca aaggcataat
      841 cgattttttc tcggtattct ttgctcatct gctcccgtgt aatttcgttg ccgtgtcggt
      901 caataaatgg gtttgttcgt tgtgggtggg ggagcttgtt gatttcactc aataagtcat
      961 cattcactaa tagcggcgtg gcggttagct gttgatgata ggcaataatc gctagagcct
     1021 ctaaaaagcc cgtagcccct gccatttcaa tattgatatg atcttcaagg tcaagagaaa
     1081 gcacaccgat aaaaaacggt ctgccgttat cgtggtgcag tcgttcaatt cggatatgcg
     1141 gaaaatctaa aaagctcaag gttgctttga taggttcatt cattaaagca gtaatacccg
     1201 ctaaattaag ctcgtgtttc attttggttt attcctttat tctttccgcc acttttaaca
     1261 gttggctaag ttgttttctt aaaatgccga gtgtgattga atcggtttcg cttggcattg
     1321 cgttatgttg ttgttccagc ttcgctattc ggttgcctag cacaattaaa agctcgggca
     1381 atgggtaagc tcggtttcag aatagaggct taaaaacgtg aatagtctgc taaataatca
     1441 ggtgttatct cagaaaaatc gagctttttc cctagtgctg cttctaattc ttgtttattt
     1501 aaaagtaaat tttggaaatc atttaaattc attgctgcca cctagaacgg aatatcatca
     1561 tcaaaattaa agctgcttga ttgtttttca tatccgttat taattgctac aaaatcaaag
     1621 gctgctttta aattagtatt atctttatca atgcttggac ttattccgcc atattgtcga
     1681 taaagctgta tatttttatc tttgagtaag cggtttaaat cgttagctgt ttcactaaga
     1741 cttgagtaat gtttacttgc ccattctatg aagtcataaa cagaaatata atcattttga
     1801 atttggctaa tctctgattg tgtttgcttg gtggttaatt tatctaacaa ggttggcgat
     1861 tgtgttttaa tcagctgtgt tatttctctt gctgttacgt aataagcatt taaatgttca
     1921 acatcaatag aattttgagc aataacatca cttaaaacgc ctttaggctg atcgaaagcg
     1981 tctttgaaat gtagtgtttt gggctcaata gcatagcttt caccatcttc aaagagagtg
     2041 caatttacaa aagcattcgt atatattcca ttttgctgaa aggaaaaaat gaggcttttc
     2101 aatttatccg ctatatgtga aaaaggttta tttgtatagt ttgataattc ttgtatcagt
     2161 tcttgaaagc tgattttggg ctcaatacga ttttcaagcc tagaaataaa ttgactgtga
     2221 tctgccataa gcgccccttt aacgtatccc tttactgtga gagcgtggaa agagaaataa
     2281 agggctttct cctttcgtgc cggttaatta ctccgccact atccacgcta atttgaataa
     2341 atccgtatag ttacggattg aacttgatag gttgtatttt gctaattatt cagataaatt
     2401 acaagtaata agctgtgttt tattacagta aatcaaaata ggctaaagat gggtttccgg
     2461 acgctataaa aaagagcatt caagcggtca tatttcatta aggttttgcg aatagtgcgg
     2521 aattttcgga gcagtacgca cgcaaggagc aaataaaaag ccgtcaatat ccatcaacgg
     2581 ctatttaaaa gtgaggtttt gacaggtagc aagtgataag ttttagccat ttttgacttg
     2641 tggaataagc actaaattga atgcccctag aattttttga atagtgtcaa agcgtggttt
     2701 gctgcctttg gctagggttt tatataggct ctctctgcct agccctgttt gcttggccag
     2761 ctccgccatt cctcttgctc ttgctacatc gttcaaggct tctaaaaatt cgtcttggtc
     2821 gccctctttt aacacttccg ctaaataggc ttgaatagtt gcctcatcgg ttaaaaactc
     2881 cgccacgtca aaagggcgta aaccgtgctt ttctgctgcc tgttcattga tttgtattgt
     2941 ttccattgct ttctcctgta gtttttaatt cagcccataa tgctttcgct ttctctatat
     3001 cggcttgctg tgttgattta tcgccgccgt gtaacaagat ataaattaca tttccttgtt
     3061 atgcgtaata aattctatat ccggctcctg ttgctatccg catttcataa agtccgcctg
     3121 taccggctaa agatttatga tcgccaaagt tgccattaat cgcatttttt atccgtcttg
     3181 ctaccgttgc ttttgcgata gggttcttta aattctcaag ccattttgaa aaattatcgg
     3241 taataataat ttgattagtg gtttgctctc tcatcattgc cggcttctta ttctatttat
     3301 tgcttattgt atccattaag atacagtgag caagggtttt tgccatttac ccacttttaa
     3361 cccactttac ccactgtcat tttttcttta tttttcagtt agttataaaa aaataatagt
     3421 gaaaagtggg taaagtgggt aaaaatcgaa aagtttacag aattttcatc aaggggaatt
     3481 acgggcataa aaaagccgtc aggcggttta cctaacggca ttacaagcgg tcatttttgg
     3541 cggaaaattt actaggctat tttcaaatga tattgcggta agctaccttg cgaacattcc
     3601 tctacaaaat ccgcccacac ttggagcagt ttaaaacgtt gctctaggcg ttcgcctcta
     3661 tcataggcgg ctttgattcg gtcgctgttt aaatgcgata aggcaacctc gattaaatcg
     3721 ctattgtagc ctttctcatt aagataagtg gaggcgatgg cccgcaagcc gtgagccact
     3781 aatttgccct tgtagcccat tcgtttgatc gcaccgttgg cggtttgagt attgacgtgc
     3841 gtttgtgggt tcttcacgct agggaataaa tacgttttac cgccacttag ctttttaatc
     3901 tctcgcaaaa gtgcgattgc ttggcgtgag agtgtgattt tatgctcaat gcctcgcttc
     3961 atattttccg ccggtatcgt ccacagtttg gctttttcgt ctatatccgc ccattttgcc
     4021 ttagccgctt cattcgggcg ggtcattgtg agtaactgcc acagaattaa atagcgtgtc
     4081 tgtaaatgaa tttgggcgtt ttgtaaggtg tataaaaact cgcctaaatc ttccggctta
     4141 atggttttga aatgttcaac cgttgggcta tcaaactctt tcgcaatatt ggcggtaggg
     4201 ttaaacggta tcatttcacg gtgtacggca aacatcataa tttcattatg tttttgaatt
     4261 gcccgtttta acttctctag tttgccctcg tcttggtatt gcttgaatgc cttgattgcc
     4321 attggtgcgg ttatttgtgt gataggcatt cttccaaagt gcggcaagag gttattttta
     4381 attaaggcga ttacatcttt ttggtagttg tctgaaaaat tcttcttggt cttgcggtgt
     4441 tccagccacg cccacgctac attttcataa gtgttgctga tttcgttggc ctttgcctgt
     4501 tcctgttgtt ggcggtgggt ttgagggtcg atattttgag ccaataatgc cctatattct
     4561 tctctaattt ctctcgcttg tgctaaagaa atatcagggt aagccccaat gcttaaatca
     4621 ttccgctttt ttgtgaacgg tctttcatac ctaaaccgcc acaatttaga atttgaggta
     4681 ataacaagta gtaagccgtt accgtctctt aatgggctgt cttccggttt cgcttttctt
     4741 atttgggtat cgtttaacgg tgtgattttt ctagccatag taaaccttta ggagtataaa
     4801 gcctaaatct taggagtatg attttttgtt tgggagtaca aaactaaaag cgataaagcc
     4861 ttatttgaaa aggctctaaa gtggttttag gagtacgaat ttttgggggt attccggcgt
     4921 actcctaaat atactcctaa aatcttcgga tttgattgta tcgtataggt acgcaacgga
     4981 caataaaaaa gccttgaaac attgattttc aaggcttttt aggtatcggt tgaaccgtat
     5041 aggattataa tttggtggag ctggggggat ttgaaccccc gtccgaaatt tctctacctt
     5101 cagtactaca tgtttagtct agtctttaat ttcgcttatt cacagcggac agacacgcaa
     5161 tgaataaact agctttattt aatttagtgt ttcgatccta aagcggcggc gtccacacga
     5221 tctcgttttg ggtgacttat ctttgccctc gtcttacgag cggaagctga ggagataagg
     5281 ctttaaagca ggttattaag ctgctaaagc gtattgttcg tcgtttgcga ctattatttt
     5341 gcggtttgtt tacgaggcct accgcacctc gacatgcacc ttgggcttcg ccaatcccgt
     5401 cgaatccaaa atcagcccca aaagtagcgg tattctaaag gaaagcggtc gaatttgcaa
     5461 aaagttttgc aagtggcgga acttagcggt attgaatagc tcgaaataaa atcatacaaa
     5521 tcccacacca gatagattta actctgttat aatgtaaacc ttgctgagag tgaagagaaa
     5581 tagaatgaaa aaatttaacg agaacgagat tgaagatgct gaaattatag atgccgagcc
     5641 gattgatgtt gatgagatag acgaagaaga aacatcggta gaagcaatgc atccggcttt
     5701 ggtttccggc tcaatgcctg tgccgcaggg caatttggat agctacatcc gaatggcaaa
     5761 tcagtatccg attctcaccg ccgagcaaga aaaagaactg gcggagcgtt attattatga
     5821 tgaagacgtt gaggcggcaa aacagctgat cctttcccat atgcggtttg tgatccatat
     5881 tgctcgtggc tatttgggct atggtttacc gctggcagat ctgatccaag agggtaatat
     5941 cggcttgatg aaagcggtaa aacggtttga cccgaatgtc ggcgtgcggt tggtttcttt
     6001 tgcggttcat tgggtgaaag cggaaattca cgaatatgtg ctgaaaaact ggcgaattgt
     6061 gaaagtggca accaccaaag ctcaacgtaa attattcttt aatttacgca aaaataaaaa
     6121 ccgcttggcg tggtttaatg aagaagagat caaaaaagtt gccgatgatt taggggtttc
     6181 agtggaagaa gtacgagaaa tggaatctcg aatgaccgga caagatttag gttttgacct
     6241 gcctgccggt gatgacgatg aagacagcta cgctccttct atgtatattg aagatagcag
     6301 ctccaatttc gccgatgact tggaagacga acaacataat gggcaggcaa ccgctcaact
     6361 tgcctatgcg ttagccaccc ttgatgaacg tagccaagac atcatcaaaa cccgctggtt
     6421 agatgacaat aaagccacac tgcaagattt agccgataaa tataacatct cggcagagcg
     6481 ggtaagacag cttgaaaatg ccgcattgaa gaaaatcaag gaagcgattt cattagacgg
     6541 ctagaaatgc ctacaagcgg tgattttttt actcgctacg ctcgcacttc gtgccgtcgg
     6601 cagagccaac gttcaaacgc aagcgtttgt gcaaaatttt acgcaaaaag caccgcttgt
     6661 attttgacta aaaatgagga tactatgatt aacattaccg ataaacaaat tgacccaact
     6721 ttctaccagc gtctaccagc gagcagacgg ctttattaac gttgccaacg ctcatttaaa
     6781 aaacattgcc ccaaaccaag tcagcaatgc gatgttgttt ggttgtgcca gatttaacgc
     6841 ctatgttgcg gcaagcaagg cggaatataa acagcagttg gccgattcca gagaagaagt
     6901 tattcaatat tttgtggagc aatacaaaga aatgctcact gcaaatttag atgaatatat
     6961 ccaaaacttc gagcgatata ttgaagggaa aaaggcggat taaccgcctt tgttttgtgg
     7021 tcaaacttgt tttacaaaat cacttaaaac ttgattaatt ctactttgcc aacctttacc
     7081 tgaagctcta aaggcttcaa gaacgctttt atcaagacgt aacgaaacca attctttctt
     7141 tgaaccagct ggtctgccac gttttttcat tgcaattgaa aactctgatg gcgtaaccgg
     7201 gcgttcatcg tcatcttttc cgtcccaaat aaaatcttgg cttgccggaa tagcttgaat
     7261 agccataagt agctgttttt tctctaaatt tttattattc aaagtattgt tgtaagtatt
     7321 cataatatgc ctctctttct gctttagatg ctcgccgaaa gctaattact ctgatgtaat
     7381 cttccctttc tgtatatgca agtgataata cgagtaaacg tccaaatgaa tatgcaaaag
     7441 attgtattct aaattcattg ttacgaataa cttcaacatc aaatcgatag ggcgatgaga
     7501 gaacttcatc tgcatcggca aaatctaatc cgtgcttggc taaattgatt ttgcgtttag
     7561 tttcgtccca agtataaatt ttcataatta ttgtatatac ataaaataaa tctaacaaga
     7621 actaaattta atttagcaaa cagtaatttg aaaatcagat tagaaataca ttttttcgat
     7681 gcagatcgca aaaataaaat gaaatttaac tgcttgaaga gtctactgag ccattttccc
     7741 cgctaccgtt aaaaaaccat ctgcggtttt aaaaatttca ccgaatcctt gagcctccaa
     7801 aatttggtcg gagacttcac gaaacgcccc ttcaccaccg gctttttgca atacccaatc
     7861 tgctttttct ttgatgtaat cgagggcgtt acgggttgca atcgcaatgc cacacacttc
     7921 aaaagccggt aaatctaagg tgtcatcacc aatgtaagcc gtttgctcag gggtaacacc
     7981 ggcttgctgc attagctcaa agcaggcgga gcgtttttcc attttgccta acaaagcgag
     8041 atcaattttc agcacctcta accgtttgcg gagcaaagcc gtatcgccgc ccgagagtac
     8101 tgccacttta atgccacaac tttgtaacat cttaacgcct aaaccatcgt gaacgtggaa
     8161 acgtttcatt acttcgcctt ggtcggtgta gtacattccg ccatcggtta gcacgccgtc
     8221 cacatcggta atcactaatt taatcttggt gagatctaac atttttttgc ctcaaatatg
     8281 gtatgatttt accattatag cttttttacc tccgtttacc aaaggaaccg aaatgtcgat
     8341 tgccgagaat ttatcgcaaa tccatcaaca aattcagcaa atttccgaac aatatcaacg
     8401 agataacgtg cgtttgttag ccgtctccaa aactaaacca gttgaggcga ttcaagcggc
     8461 gattgaggca gggcagcgtg catttggcga aaattatgtg caggaagcgg tcgaaaaaat
     8521 cgaatttttt gcaaatcgcc ccgaattaga atggcatttt attgggccac ttcaatcaaa
     8581 taaaaccaaa ttggtcgctg cctattttga ttggattcaa acggtggata gattaaaaat
     8641 tgccgagcgt ttaaacgaac aacgcccagc ggataaagcc ccgctcaacg tgctgattca
     8701 aattaatatc agcgatgaag cctcgaaatc gggcattgaa ccagatgaaa tgctaccact
     8761 tgcccaagcg atttctcaat taccgaattt gaaactgcgg ggcttaatgg ccattcccaa
     8821 accagaaagc gagccagagc aacaaaaagt tgcctttcgc aaaatgcagc tactttttaa
     8881 ccgcttgcaa acagaatttg acgacattga taccctttca atggggatgt cggacgatat
     8941 gcaagcggcg attgaatgtg gctcgacaat ggtgcggatt gggactgcga tttttggggc
     9001 gagacgttag ccagctgagt ttttacataa agctgataca agcggtcaaa ttcgccaaaa
     9061 actttgcaaa ttccaccgct tgttttgtag ccaacagcgg aaaaagtctg tataattacg
     9121 cctcctaatt tcttgactct cgccagtttt gatgggcgag agtttttatt ttgacttaac
     9181 ttaaagatat tccaatgcaa aatgtagata tttcaaaatt gcgtaacatt gcaattattg
     9241 cccacgttga ccacggtaaa accaccttgg ttgataaact tttacaacaa tcaggcacct
     9301 ttggtgaaac tcgtggtgac gttgatgagc gtgtaatgga ttccaacgat cttgaaaaag
     9361 agcgtggcat taccattctt gcaaaaaata cggcaattaa ctggaatggt tatcacatca
     9421 atatcgtaga taccccaggg cacgctgact tcggtggtga ggttgagcgt gtactttcaa
     9481 tggtagattc tgttctgtta gtggttgatg cctttgatgg cccgatgccg caaacccgtt
     9541 tcgtcacgca aaaagcgttc gctcacggtt taaaaccaat cgtagtgatc aataaagttg
     9601 accgcccagg tgctcgccct gattgggtgg tggatcaagt gttcgacttg tttgtaaatt
     9661 taggtgcaag tgatgagcag ttagatttcc caattatcta cgcttcagcc ttaaatggtg
     9721 tagcaggctt agaacacgaa gacctagcgg cagatatgac cccactttat gaagcgattg
     9781 tcaaacacgt tcaaccacca caagtggaat tagatgcacc attccaaatg cagatttcgc
     9841 aattagacta caacaactat gtgggcgtta tcggtattgg acgtatcaaa cgtggttcgg
     9901 tgaaacctaa ccaagcggta acgattgtag atagcgaagg caaaactcgc caaggtcgta
     9961 tcggtcaggt gttggggcat ttaggtttac aacgttttga agcaacagaa gcctttgctg
    10021 gcgatattgt tgcgattact ggtttaggcg aattaaatat ttcagatact atttgtgata
    10081 ttaacgcagt agaggcttta ccagcattaa gcgttgatga accaacggta acgatgttct
    10141 tctgcgtaaa cacctcgcca ttctgcggtc aagaaggtaa atttgtgact tcacgccaaa
    10201 ttcttgagcg tttaaacaaa gaattagtcc acaacgtggc attgcgtgtg gaagaaactt
    10261 cgaacccaga cgaattccgt gtgtcaggtc gtggcgaatt acacttatcg gtattaatcg
    10321 aaaatatgcg tcgtgaaggt tacgagttag cagtgtctcg cccgaaagta atctaccgcg
    10381 aagaagacgg caaaaaacaa gagccgtttg agcaagtgac gattgatatc gaagagcaac
    10441 accaaggttc agtgatggaa gcacttggta tccgtaaagg tgaagtgcgt gatatgattc
    10501 cggacggcaa aggtcgtacc cgtttagagt atgtgatccc aagccgtggc ttaatcggtt
    10561 tccgtaacga atttatgacg atgacctcag gtactggttt actttactct acattcagcc
    10621 actatgatga cgtaaaaccg ggcgaaatcg gtcagcgtaa aaacggtgtg ttaatttcta
    10681 acgcaacggg taaggcatta gcatacgcat tatttggctt acaagagcgt ggaaaattaa
    10741 tgatcgacca cggtgttgat gtatatgaag ggcaaattat cgggattcac agccgttcaa
    10801 acgatttaac cgtaaactgc ttacagggta agaaattaac caatatgcgt gcatcaggta
    10861 aagacgatgc catcgtttta accacaccgg tgcgtttcac tctagaacaa gcgttagagt
    10921 ttattgacga tgacgaatta gtggaagtaa caccacaatc ggtgcgtatt cgtaaacgtt
    10981 tattaactga aaacgaccgg aaacgtgcaa gccgtactac aaccagtacc agcacgcact
    11041 aactgataaa aaagagcgac tgaggtcgct cttttggttt taaattctcc ctacaaatat
    11101 aacgccatta ccaccgcatt ttctttgcta ccgtttggcg tggggtaata gtttttgcgg
    11161 gtggtaactt cattaaaacc gagtgaatcg tagagttttc tggcagaatg gttggattct
    11221 cgcacttcca accacagggt ggtgagtttc cgttgttgca gttgcaaaat taattcggaa
    11281 agtaaccgct tgccaatgcc tttgccttga aaatcaggag caactgcaat gttaaataag
    11341 gttgcttcat ctagcacgat atgactaatg gcaaaagcca caattttgtt ttgctctacc
    11401 acttttaaat tcaggtattt ttcaccctga ttatttaata acgtcccctt tgaccacggc
    11461 actaaatggg cttgttgctc aatttcaaat aactgctcga aatcttccgc ttgaatttgc
    11521 tcaatcacgc tgttctccaa attgtgccga aaaagtatca atttgttgcc aaagttggcg
    11581 tttggtcgta gatttggcta aatcttgcca agactcgttt ttccaagcgg tctgattagt
    11641 aaaacttttt gccattttga ccgcttgttc ttccgcttga atcagccaaa agatcggcga
    11701 atgctcaaac accaaacgtt gtgattgttc gccagtcagc cattgatatt cgctgttttt
    11761 taactttaaa gcacgcagaa tatcggcaaa gagccggctg tgttggtgat cttcctcaca
    11821 cactaccacc aattttaccg cattgtttag gcggatttga gcatcgcctt ttagcacttg
    11881 gggattggtt agcacccatt gcgaaatacc catttcctgt aacagtaaat cacgacgatt
    11941 catttaaaac ctcatcaaat tttgcaaata gtatcaaaac tccgcttgaa cagaaacttt
    12001 tttcaaaaaa ttcaagattt ctgtacttaa attcggtata ttcacgctaa attttttaga
    12061 taaggtaaga aaatgctctc tttagaaagc gaagtacttg aacgccattt ggcgttattt
    12121 gaaaacaagt cggtgttatt atttggcgat gtgcgagatg attttgcccg ccagctgcaa
    12181 tctcgtgcgg aaaaggttgc ggtattcagt agctattttg attacgcacg ccaccatagt
    12241 gaggttgaat ttggcttaga atgtggtttg agtgccgagt tagcggtgtt ttattggacg
    12301 aagaacaagc aggaatgcca attccaactg ctacaatggc tgtcgcaatg ccaagcaggg
    12361 caagaaatgt tgattatcgg cgaaaaccga gctggtgttc gttcggtaga aaaactgctc
    12421 gaaccgttcg gcaatattgc caaaatcgac tctgcacgcc gttgtggttt ataccatttt
    12481 gagctacaga ccgtgccgac attcgattgc aaaaaattct ggaaatccta ccgcttgcaa
    12541 gacttagatg tgttcgatct accagcggtt tttagctcgg cggagctgga taacggcacc
    12601 aaattattgc tttcaacctt cgacaaagca gacaggctaa aaggcaaagt gttagattta
    12661 ggctgtgggg ctggcgtgat tggggcaacg ctcaaacaac aatttccgaa aatcaaactg
    12721 acgatgagcg atattcacgc aatggcgttg gaatccggca gacgtacttt ggcggaaaat
    12781 cagttagagg gggaagtggt agcaagtgat gtgttctctc acattaatga acggtttgat
    12841 ttaattgtct ccaacccacc attccacgac ggggtcgata ccgcctaccg agcggtggag
    12901 gagttgattt tccaagctaa aaatcaccta accaaaggcg gtgagctgcg aattgtcgca
    12961 aacgcattct tgccttaccc agatttgctc gataaagcct tcggcaaaca cgacgtactg
    13021 gcaaaatcca ccaagtttaa ggtgtattcc gcaagaacat aataggcaga acaaacggtt
    13081 ataaaaaagc cggtatttgc ttaaaaaagt gcttgatatt tctgccggct tgacgtaaat
    13141 tctgttcata ctgttttgga gtaatgcaat gaatttaaac tacactttac atcaccacca
    13201 cactttaacc tgatatcttt cgggcatttg gtgtgtgcgg aagataactc ttccgagcaa
    13261 gtgtagcaaa tcaataaaga tctctcggaa ggcaactttc gagagatttt tttatagata
    13321 aaatttggga ataatatgaa aaacccgatt gaaatttacc aaagccaaga cggcaaaacc
    13381 caagtttccg tgcagtttga taaggaaacg gtgtggctaa gtttacagca aatggcagat
    13441 atttttggac gagataagtc tgttatttct cggcacttac gaaatatcta ccaagaaggt
    13501 gaactggagc gtgaggcaac tgttgcaaaa aatgcaacag ttcaaattga aggtgctaga
    13561 gaagtcgttc gcctaaaaga ctatctggtg aaaggctatg cccttaacga acgccgtttg
    13621 caggaacgag gtatcgagtt tgagcaggtg attggtttgc tcagtcaata cactggttgt
    13681 cttagccttg ttggtggcag aaagtttgcc tgaacaaaaa gaactgatga ttaaactgat
    13741 tgaacacttt attttattaa aagcataaca ggaaaaaata tgagtattaa tatttcgccg
    13801 ttagcctcca aaaatctcac tgaatggaaa ctattgtggc aacaatattt agatttttat
    13861 caagtgaatt tagcagatga tattattcaa tcaacttggc agaaagtttc agagagtgaa
    13921 aatgtaaagg gatttggggc ttttgttgat aacagaatgg ttggttttgt tcatattgtg
    13981 attcacccaa atacttggaa tttaactgaa tgctgttatt tggaagattt atttgtttta
    14041 gctgaatatc gcaaccaagg cataggaaaa gaattaattg aatatattta tcgata
//
LOCUS       DANLXQ010000056        13911 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.056, whole genome shotgun sequence.
ACCESSION   DANLXQ010000056 DANLXQ010000000
VERSION     DANLXQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13911
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.056"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..790)
                     /gene="nfo"
                     /locus_tag="PXD94_001276"
     CDS             complement(<1..790)
                     /gene="nfo"
                     /locus_tag="PXD94_001276"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL6286145.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETI"
     gene            849..1418
                     /locus_tag="PXD94_001277"
     CDS             849..1418
                     /locus_tag="PXD94_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL6286146.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEAVE"
     gene            1448..1831
                     /locus_tag="PXD94_001278"
     CDS             1448..1831
                     /locus_tag="PXD94_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286147.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
     gene            complement(1912..2520)
                     /locus_tag="PXD94_001279"
     CDS             complement(1912..2520)
                     /locus_tag="PXD94_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL6286148.1"
                     /translation="MKLTKIALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGT
                     TSVQMSENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKF
                     QENQTVAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPL
                     EAKGQIVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKNIK"
     gene            2658..3557
                     /gene="corC"
                     /locus_tag="PXD94_001280"
     CDS             2658..3557
                     /gene="corC"
                     /locus_tag="PXD94_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL6286149.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            3616..5124
                     /gene="lnt"
                     /locus_tag="PXD94_001281"
     CDS             3616..5124
                     /gene="lnt"
                     /locus_tag="PXD94_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL6286150.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(5170..5634)
                     /locus_tag="PXD94_001282"
     CDS             complement(5170..5634)
                     /locus_tag="PXD94_001282"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL6286151.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
     gene            complement(5840..7039)
                     /locus_tag="PXD94_001283"
     CDS             complement(5840..7039)
                     /locus_tag="PXD94_001283"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL6286152.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            complement(7058..7705)
                     /gene="ribE"
                     /locus_tag="PXD94_001284"
     CDS             complement(7058..7705)
                     /gene="ribE"
                     /locus_tag="PXD94_001284"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL6286153.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETDLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            complement(7922..9019)
                     /gene="ribD"
                     /locus_tag="PXD94_001285"
     CDS             complement(7922..9019)
                     /gene="ribD"
                     /locus_tag="PXD94_001285"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL6286154.1"
                     /translation="MTDVQYMAYAITLAEKARGWTSPNPLVGCVIVKNGNIIAEGYHQ
                     KAGSWHAERNAILNCEEDLTGATAYVTLEPCCHHGRTPPCSELLIERGIKKVFIGSRD
                     PNPLVSGKGAAQLRAAGIEVVEDFMRTECDALNPIFFHYIQTKRPYVLLKYGMTADGK
                     IATYTGNSKWITGEAARANVQKSRHEYSAIMVGVETVLADDPMLNSRMENAKQPVRIV
                     CDSQLRTPLDSQLVQTARQYRTIIATLNNDAKKQAEYTACGVEILVTGERDKRVDLAD
                     LLQKLGELGIDSLLIEGGSQLNFSVLEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIG
                     ELAQAVKLRLKSTELLGEDILLDYEVDLLRN"
     regulatory      complement(9195..9313)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9517..11919
                     /gene="lon"
                     /locus_tag="PXD94_001286"
     CDS             9517..11919
                     /gene="lon"
                     /locus_tag="PXD94_001286"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL6286155.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDMYGVGVIANIIQMLNLPDGTVKVLVEGQTRAKIEQ
                     IHDDENGFWAAIQPIYSEYDDENEELKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKI
                     TLEDRLADTIASNLIAPVKKKQELLEQPNLIARFEALLIAMATEMDTLETETRIRNRV
                     KQQMEKNQRDYYLNEQIKAIQKELGNGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTE
                     FKKLKAMPQSSSEATVVRGYIDWILQMPWHKKSAVKKDLAQAQEILDKDHYGLERVKE
                     RIVEYLAVQSRLNKLKGPILCLVGPPGVGKTSLGQSIANATGRKYVRMALGGVRDEAE
                     IRGHRRTYIGSMPGSLMMKMAKVGVRNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQ
                     NKAFNDHYLEVDYDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKMHIARGHL
                     LAKQQENNGLKEGELTIEDSAILSIIRYYTREAGVRSLEREIAKICRKAVKALVLDKK
                     LKSITVSEKNIEEYLGVKRFDYGKMDSQNRIGEVTGLAWTEVGGDLLTIETASVAGKG
                     KFSYTGSLGDVMKESIQAAMVVVRSRAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSA
                     GIAMCTALISSLTGNPVRKEVAMTGEISLRGKVLPIGGLKEKLLAAHRGGITTVIIPK
                     ENEKDLEEIPENAKAALDIHAVETIDEVLALALENPPMGIEILSKAEIKVKKSRAKAT
                     VQ"
     gene            complement(12053..12943)
                     /gene="ilvY"
                     /locus_tag="PXD94_001287"
     CDS             complement(12053..12943)
                     /gene="ilvY"
                     /locus_tag="PXD94_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL6286156.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGPVRKRIDRWFKEQKIKDPI
                     IYATVSGHEAIVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGICVQQ
                     KRLQEPIINAFWQLLSDNSA"
     gene            complement(12945..13901)
                     /locus_tag="PXD94_001288"
     CDS             complement(12945..13901)
                     /locus_tag="PXD94_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6286157.1"
                     /translation="MILSEIPNKILFLRHDGKIGDYVVSSFVFREIKKQSPQTQIGVV
                     CSQKNRYLFENNPYIDKLYLVKTKSIPDYIRGGKQLAKEQYDVVIDPTVTLRNRDLLF
                     LRTISAKHYVGYQKQKYRLFDLNVEDRKQHFSEIYQDALALIGFENIETQYDVPLNAT
                     SNQSIKHYLYENQIRNYIALNLFGAGSARRFSEQKINELLSCLKQHSDKPIVLLTFPE
                     VTAKLCSFTKKYKNIFVYEKTKSVFDSIELIRFADLLISPDTATVHIASGLSKKIIAF
                     YSSDEQNFIHWHPNNQAETHILRFRKSVNDLDFAAIKPEWLK"
BASE COUNT         4055 a         2989 c         2746 g         4121 t
ORIGIN      
        1 aaattgtctc taaaatcata ggaatatggt taaaatgcat attttgcata ataaatgcaa
       61 acacttcggt tcctattgtg ccttctctta aggtatgatg acgatccact ctgctgccta
      121 atggtgtttt tgaaccattt aagtgcattc cccttaaata atgaaaacca accgtttggt
      181 taaaatcatt aaatactgcc tcgctctttt cataagaact aatatcatag ccggcagaaa
      241 ataagtgaca agtatctaaa cacaccccca ctcggctttt atcctcaact tgctcaataa
      301 tagttgccaa atgatcgaaa tgccaaccta aattagaacc ctgtcctgcc gtattttcaa
      361 tcacggcaat aacattcggt actttttcaa ctgcaatatt gatcgattct gcaattctag
      421 ccaaacaatc ttgctcggaa atttgcttta aatgcgcccc cgggtgaaaa ttcaacaatt
      481 ttaaacctaa ttgatttgct cgagacatct catcaataaa tgctacacga gatttctcca
      541 atgcctccgc ttccggattt cctaaattaa tcaaatagct atcgtgtggt aagatttgct
      601 ctgctgaaaa atgatgcact ttacaaaaac gcttaaattt ttcgatagtt tcggctttta
      661 atgccggtgc ctgccattga cgttgattct tagtaaataa cgcaaatgca ttagcaccaa
      721 tttctacgga acgcaaaact gcattttcca ccccacctga agcactaacg tgtgcgccta
      781 tatatttcat tcagtcctct cttgtgttct gctatactaa caattctttt ttctatctat
      841 ttcaaattat gaacgaaact accattaacg atttttccct gctttgccgt ttgtttggca
      901 acttatttta tcgtcaccct accgacccta ttttagctgg cacatttaac tggcttgccc
      961 aaggaggatt acgccaagtc tgggcattaa atacggatag ccaaagtgaa aatgcgctga
     1021 ctctcttaca aaagaatgct aaccctacgc aattagcagc aagctatcaa gcgctttttg
     1081 ctgaaaatgg ggctatttct ccaacaattt cagcctatgg attatcagtt aataattttg
     1141 tagcttttag acaagagcgg gcaatgcctg aattagaaaa tgccgatcac attgggttac
     1201 tatttctcac ggcatcttgg atcgaagata atctcgattc tgccttagct caaaaaatat
     1261 tattcgaaca atttttacta ccttgtgcag gcaaattctt aggaaaaata gaagctttcg
     1321 acaatggttt ttataaagcc ctcgcacaac ttagccgtga tgcccttagt gctatggctg
     1381 atgaacttga agaaagtgag ttagaagcgg tagaataaat caccaatttc aactagagga
     1441 ttcagcaatg aaaacactca aattaggctg cattttttca ctcgcactta tgttaagtag
     1501 ctgtacttta actccagagc aacaagctga acgtaaagcg aagcaaattc gggcagaaca
     1561 agatttgcaa gttcagctcg ccaaacaatg tgatgtagaa acggcggagt taatgtacca
     1621 acaatttaat ccgccgctta gccaaaccga acaagaagag aaagcattta agaaacgcta
     1681 tgccgaaaaa gtcaatgccc caatgttcca agcctgttat aagcttgctt ggcaaaatta
     1741 caaggcacaa gtagaacttg aagaaattcg ttggaattac gatagagact atatgtatag
     1801 aggctggcgt tactgctatt attgctggta aatctcattg tatgagcggt cattttttta
     1861 acaccttgca aaaaattggc gagaaatgac cgcttattaa ttgatatagg tttattttat
     1921 atttttcttc gcccattcta agaaacgttt ttgggtgtct ttatctgcct ttctaaataa
     1981 aagctgtaat tcttcttgtg aaactttatt tgtatccacc acaatttgtc cttttgcttc
     2041 aagaggtgcc gtcgcaaact gttcaactga agctgttttt ttcgctaagt tatatttcgt
     2101 taaattatct tcaactcttg ttttcgcaag gaaaccttcc ccttttaaga aatcttgttt
     2161 atgtgaaata accttacctg aatcggtttt aattgccaca gtctgattct cttggaattt
     2221 acgaagatct aactttgaat ttaatcttgg cgtttctaat tttgcatttt ccgcactgcc
     2281 ttggaaagtc aaaatgattt gatcagaggt gaaaaaatcc ccttcaactg tcccaccgac
     2341 ttccactacc acttgatgcg ttttattctc actcatttga accgaagtcg tgccggattt
     2401 aacacgttgc ccgtcaaagg ctaaaatgtc caccattgat gatgtactta acgtcccggc
     2461 cacactcatc gaactgacaa ctaaggctgt taaacttaat gctattttgg ttaatttcat
     2521 ttcaaactcc taaaatatga agaaataaaa agatttttgc ttatcctatg ctaatttgag
     2581 tgaaaatagc aatgttttat ttgctttgtg actgaatttg cttattataa gcgtacttta
     2641 tttaagtgga acaaactatg agtgatgaac agcaaagtgt aaacaccacc gaaaaatcag
     2701 aaaaaaaatc gattttacac tctctattta gcgggctttt tcaaaacgag cctaaaaacc
     2761 gtgaagatct agtcgaagtg attcgagact ctgttgaaaa tgaattgatc gacagcgaca
     2821 ccaaagagat gatcgaagga gtaatggaaa tctcggaatt acgagtgcgg gatattatga
     2881 ttccccgtgc gcaaattgtg tttattgatg ccaatgaacc tttagatgtc tgtattgatt
     2941 taattattga atccgcacac tctcgttttc cggtgattcg gggtgagaaa gatactatcg
     3001 aaggtattct tcactctaaa gatttattga aatatttacg ttctgactct gaacccttta
     3061 atctgcaaca ggtattacgc cctgcggtta ttgtaccgga aagcaaacgc ctagaccgaa
     3121 tgctaaaaga gttccgttca gaacgcttcc atatggcagt agtggttgat gaatttggtg
     3181 cggtttcagg cttagtcacc attgaagaca ttcttgaaca aattgtgggt gatattgaag
     3241 atgagtttga tgaagaagag gtcaaaccaa ttcgccagct ttcaagacat acttatgcgg
     3301 tattaccgct caccgacatc aaaaaattca atgaattttt tgctaccgac tttaatgacg
     3361 aagaggtgga taccattggt ggtttagtga tgcaggcttt cggttatttc ccgaaacgag
     3421 atgagcaaat tgagttaaac ggaattgatt ttaaagtcac ctcagctaat agccgccaat
     3481 tgattcagct acgagtcaca ctttccgatg aacagttaga gaaaatggaa aaaacctctc
     3541 ctaacgaaga agaataatat agtgagtttc aatcaaggca cgtttcggcg tgcctcattt
     3601 atttagtgag ttattatgaa ttttgcaaaa aatccgcctt ttttaaccgc ttgtctcctt
     3661 gcaatcatac tgggtgcaat cggcactttc gcctattcgc cttttgatat ttggattctt
     3721 gcctttgctt ccgcagccgg cttggtttgg tcagccactc tggcaaacag aaaaaccgca
     3781 ttatgggcaa cctttgcttg gtctatcggc tacttctgca ccggtgtaaa ctgggtttct
     3841 gtgagtatga cccaatttgg tggtgttcct accatagtaa gctacatagc agtattcctg
     3901 ttggcgtgct atctcgctat ttataatctg ctgtttagtg tagtttcaca caggctcaaa
     3961 ctgaataatc cgtttgcatt ggctgcgatt tttaccttta ccgaatattt acgaggcgtg
     4021 gtatttaccg gttttccgtg gctacaattc ggctatagct taatagacag cccctttgct
     4081 ggtattgcgc cgattttggg cgttgaaggt ttgactttct tggtaatagt tgcaagtagc
     4141 tatctagtga aaattattca gcaagagaac aaaactgtgc catcattgat ggtactagcg
     4201 gtcatttttg gcttaagttt tgccactcgc tttctccaat ttgtgcaaat tgatacagaa
     4261 aaacaaccgc ttgtagtgag tttagtacaa gggaatattg agcaaaaaat gaagtgggat
     4321 ccgcaacatt tcaaccacag cgtacgaacc tacgaacagc tcattctgcc cctattaggt
     4381 aacagtgaac tgattatttt gcctgaatcc gccattccgg cacttgaaaa tcaaatcagc
     4441 ccgctgcttg gctcgctcga tcacttagct cgccaacacg gaagtgaaat tattatcggc
     4501 acactctatc aaaatgagca agaggaactg tttaacagta gcgtgctatt aggcgaaaaa
     4561 gagagggaat atgccctgca cagcaaccct cgctacaaca aacatcacct tgtgccgttt
     4621 ggcgaatatg tgccgtttgg ctcggtgctg gattggatgc gggaagtgtt tattctacca
     4681 attaatcttt cacaaggcag tttcgttcaa ccatcattct tggcgaaaaa caaccgattt
     4741 aatatggcga tttgctatga aattattttt ggtgatcagt tacaacaaaa ccaaaaaagc
     4801 caaaattcag attatttatt aactatcacc aatgatgcgt ggtttggttc ttccatcggg
     4861 ccttggcaac atttccaaat ggcaagaatg cgagcgttag aacttggcaa accattgctt
     4921 cgagctgcga ataccggtat taccgccatt gttgatgcca acggaaaagt aattgaacaa
     4981 ctaccgcaat ttactgctga tgcactaact gccaccattg tgccaaccaa aggcgatacg
     5041 ccgtttaaac aattaggtca ctggttaatt tacagtttca gcctattttg tgtcattgcc
     5101 agcctgttac tacgcagaaa ataaaaaaat ccccttaggc tcttgggtct aaggggattt
     5161 tatcgctgat tacgctaagg cttttaatag gttcgccatt tcaatcgcta ccattgcaca
     5221 ctcttcgcct ttattaccgg ctttagtgcc tgaacgctca atcgcttgct ctaaattttc
     5281 caccgttaaa ataccgttaa taatcggcac gccggtttct aaagcagaag agctgatgcc
     5341 ttttgctgac tcgttcacta ccacatcata gtggctggtt gctccacgca cgaccgcacc
     5401 taagcagata atcgcatcat aacgaccgct ttgtgccatt tttttcgctg caattgagat
     5461 ttctaatgca cccggcaccc atacggtgtc gatatttttt tcttctacgc cgtgacgcaa
     5521 taatttatcg gttgcaccgt ttaacagttg atcgataaaa atcttgtgcc agttagttgc
     5581 cacaataccg aattttaaac cagttgcgat gtagtttcct tgaaatgttg ccattgtttg
     5641 ttttccttct ttaaaataaa tttgatactc aataaccctc tccccttgtg ggagagggat
     5701 agatttttct tcgttcagaa gaaaaatcag ggagagggga aattgcaaac tttttctaaa
     5761 aaatataccc tctctctgaa aaatacttct gaacgaagta tttttctgtc tctctcccac
     5821 aaggggagag agtattggat tagaaattaa acatatgtcg cattttgtgc tgtttaactt
     5881 taaggtattc caaatcgtgt tcgtttggct caaccataat cggctcacga gccactacgt
     5941 ttaaaccttg ctcaatgagt ccttcaattt ttgccggatt attggttaac aaacgaatcg
     6001 attttacgcc taatttttca aacatttgag cggcgatata atattcacgc tcatcatctt
     6061 tgaaacctaa tgccaggttg gcttcaaccg tgtccattcc tctatcttgc aattcgtaag
     6121 cacgcagttt attaattaaa ccgatgccac gtccttcttg gcgtaaataa agcaccacgc
     6181 ctctgccctc ttgctccact tggttcattg ccgctgcgaa ctgctgacca caatcgcaac
     6241 gttgtgagcc aaaggcatcg ccggttaaac attcggaatg gatacggcat aatacatttt
     6301 cgccatcacc aatatcacct ttaactaaag cgacgtgttc tttgcctgaa atagtttcaa
     6361 cgaaactgtg tgctttgaac tcaccgtatt tggtcggcat ttttaccacc gaaaccacat
     6421 tcaccaagct gtcgtgtttt ctacgatact cttgtaactg ctgaatagta ataaacggca
     6481 tattgtgtgc ttgtgcaaat gcctgtaatt ccggcatctg catcatcgtg ccgtcttctg
     6541 ccataatttc acagcaaagc cctgccggtt tcaagcccgc caaacgagct aaatccacag
     6601 ttgcttcggt atgaccgtta cgcactaata cgcctccgtc ttttgccact aatgggaaaa
     6661 catgccccgg acgtctaaaa tcttccggct tagcattttc atcaagcatt ttcaggcaag
     6721 ttaatgaacg ctcaaaagcg gaaataccgg tgccggtttg aatatggtca acagaaacgg
     6781 taaatgcggt ttgatgatta tcttcgttct ctagcaccat cgggtgaaaa tttaattttt
     6841 tggcaatttc tgtcgaaatc ggcgtacaaa tcaacccttt accatatttc gccataaagt
     6901 tgatattttc cggtgtcgca aattccgctg cacaaatgaa gtccccttca ttttcacggt
     6961 cggcatcatc gctgactaaa ataattttcc cttgtgcaat tgcttgaatt gcttcttcta
     7021 ccgttgaaaa cttaaacatt atttgtcctt tatttggtta aaaccctgca tttttaagaa
     7081 agtccgcagt aattttactt tgcggatttt caggctcttt tttcaataaa aactgttcga
     7141 tatatttgcc gacaatgtca ttttctaaat tcaccaaact accgattttt ttcgtaccga
     7201 ggtcggtttc cttaatggta tgcggaataa tcgatacccg gaaagattcc gcatcaacat
     7261 ccaccaccgt taaactaatg ccatcaatag taattgagcc tttttcaatg atgtaacgca
     7321 ttaacttagg ttcggcttta atgcgatacc aagttgaatt atctgctggt gtaatctctg
     7381 caaccacacc cgtgccatca atatgacctg acacaatatg cccaccaaaa cgcccgtttg
     7441 ccgccattgc ccgctcaaga ttaaccgagc tgttgatact taattcgcca agtgatgtac
     7501 gttttaaggt ttctgacata acatcagcag taaattgctc ctgcgtaaat gaagtcacgg
     7561 ttaaacacac gccatttacc gcaatgctat cccctaaatg tacatcggtc agaatctttt
     7621 ttgctttaac cgttaccacc acaaactcgc cttgtttctt aatttgggct atctggccaa
     7681 cttcttcgat aatgcctgtg aacatttaaa tttttctccg atttacttcc aatttagtct
     7741 gagaactgct tatcttacta ttcccctctc ccgtttacgg gagagggaag agaaaatccg
     7801 taggattttc ggagggagag ggaagattac actcattttc ccctctctct gcataaaatt
     7861 tctgaacgaa attttatgct gtctctctcc cgtaaacggg cgagagtaag gaaatttcaa
     7921 tttaattacg aagcaagtcc acttcataat ccaacaaaat atcttctccg agcaattcgg
     7981 tggattttag acgtaattta accgcttgtg ctaactcgcc aatccccttg ccaccaatcg
     8041 gggtttttgc cgctttgccg ccaatcagtt tcggggcaat atagcaatgt actcgattga
     8101 ctaacccctg ttccagcacg ctgaaattga gttgcgaacc accttcaatc aataagctgt
     8161 cgatcccgag ttcgcccagc ttttgcaaaa gatcggctaa atccacccgc ttgtcacgtt
     8221 cgcctgtcac taaaatttca acaccgcaag cggtatattc tgcctgcttt tttgcatcat
     8281 tattgagcgt agcaataatg gtgcgatatt ggcgtgcagt ttggactagt tggctatcca
     8341 atggggtacg cagttggctg tcacatacaa tccgcaccgg ctgtttagca ttttccattc
     8401 ggctgttgag cattgggtca tcggcaagca ccgtttccac cccgaccata atcgcactat
     8461 attcgtggcg gcttttctgt acattagctc gggcagcctc gccggtgatc catttcgaat
     8521 tccccgtata agtcgcaatt ttaccatctg ccgtcatccc atatttcagc aacacataag
     8581 ggcgtttggt ttgaatgtaa tggaagaaaa tcgggttcag cgcatcgcac tccgttcgca
     8641 taaagtcttc cacaacctca atgccggcgg ctctaagctg tgccgctcct ttgccagaaa
     8701 caagtgggtt cggatctcga gagccgataa acactttttt gatcccacgt tcaatcagca
     8761 gttcggaaca aggcggggtt cgcccgtggt ggcaacaagg ctctaaggtt acataagcgg
     8821 ttgcccccgt cagatcttcc tcgcagttta aaatcgcatt gcgttcagcg tgccaactac
     8881 cggctttttg gtgataccct tcggcgataa tgttgccgtt tttcacgatc acacagccaa
     8941 ctagcggatt agggcttgtc caaccccgtg ctttttcggc aagggtgatg gcataagcca
     9001 tatattgaac atctgtcata tacattttga taaattgagg gcgtaatgaa aggattattg
     9061 caatacaagc ggtcgttttt agtaaaaaat ttgcaaacgc cgcctgcaaa acaatgaagc
     9121 cttgaaatct cacgactcaa ggcttgcaaa aaagacacaa aaaatgaccg cttgtgcggt
     9181 attttttgcg gtttttgact cttctttcat ccagactata ctgtcggttt tggaatttca
     9241 ccaaatcctg ccgattgctc ggctcgcgga ctttaccgcc gatcgggaat ttcaccctgc
     9301 cctgaagatt aaaatatgca ggggattttg ctcgcattac gatttgatgt caagcgggaa
     9361 attaagctaa ttaccctgat tttaacaatt gattaaatcg ccaacatcta ctgttttgac
     9421 tattcgagtt tttttaagat aaatcaaatt ttcccttgtt tttcccaaat tcgccctcat
     9481 aatgggggat tatattttga tagagagagc aaatttatgg caccaagaaa aaagaaagcg
     9541 attgaattac cactactccc attgcgtgat gtggtggttt ttccttatat ggttatgccg
     9601 ttatttgtag gacgtgaaaa atcaattcaa gccctacgct cggcgatgga ttccaataaa
     9661 caactgtttt tagtcactca gcaagatcca aataaagaag agccgaatgc agaagatatg
     9721 tatggcgtag gtgtaattgc caacattatt caaatgttaa acctgccgga cggcacagtt
     9781 aaagtgctgg tagaaggaca aacccgtgcc aaaatcgagc aaattcacga tgatgaaaac
     9841 ggcttctggg cggcaattca gccaatatat tcggaatatg atgacgaaaa cgaagagtta
     9901 aaagcgattg ctaaaaccac tttaaccgaa tttgaaaatt acgttaaaaa taacaagaaa
     9961 attccggcgg aaattattgc taaactgcaa aaaatcacgt tggaagaccg cttggcggat
    10021 accattgcct ctaacttaat tgctccggtg aagaaaaaac aggaattatt ggagcaacca
    10081 aatctgattg cccgttttga agccttgtta attgcgatgg caactgagat ggatacgctt
    10141 gaaaccgaaa ctcggattcg taatcgtgtt aaacagcaaa tggaaaaaaa ccaacgtgat
    10201 tactacctca acgagcaaat taaagcgatt caaaaagagt taggtaatgg cgatgacgtt
    10261 gaacaaagcg agttagataa gcttaaagag aaaattgacg ctgcaaaatt accgaccgag
    10321 gtaaaagaca agctgaatac ggagttcaaa aagctcaaag caatgccgca aagctcttcc
    10381 gaagctaccg ttgtgcgtgg ttatattgac tggattttac aaatgccgtg gcataaaaaa
    10441 tcagcggtca aaaaagattt agcccaagct caagagattt tggataaaga ccactacggc
    10501 ttagaacgag tgaaagagcg gattgtagaa tacttagcag tgcaaagtcg tctaaataaa
    10561 ttaaaaggtc cgattttgtg cttagttggt ccgccggggg ttggtaaaac ctcattaggg
    10621 caatctattg ccaatgccac agggcgtaaa tatgtgcgta tggcgttagg tggcgtgcgt
    10681 gatgaagcgg aaatccgtgg gcatcgccgt acttacatcg gctcaatgcc gggtagccta
    10741 atgatgaaaa tggcgaaagt cggcgtgcgt aatccgctgt tcttgttaga tgaaatcgac
    10801 aaaatggctc aagatatgcg tggcgacccg gcttctgctc tgcttgaagt gttagatcct
    10861 gagcaaaata aagcctttaa cgaccattat ttagaggtgg attacgattt atccgatgta
    10921 atgtttgtgg caacctcaaa ctcaatgaac attccgccag cattgctcga ccgtatggaa
    10981 gtgattcgtc tttcaggcta taccgaagat gagaaaatgc acattgctcg tggtcattta
    11041 ctggctaaac agcaagaaaa taacggacta aaagagggtg agctgaccat tgaagacagt
    11101 gcaattttaa gtattatccg ctactacacc cgtgaagcag gcgtgcgtag ccttgagcgt
    11161 gaaattgcga aaatctgccg taaagccgtg aaagctttag tattggataa gaaattaaaa
    11221 tccattacag tttctgagaa aaatatcgaa gaatacttag gcgtaaaacg ttttgactac
    11281 ggcaaaatgg atagccaaaa ccgcattggc gaagtgaccg gtttagcgtg gacagaagtt
    11341 ggcggtgatt tattaaccat tgaaaccgct tctgttgcag gtaaaggcaa attctcttac
    11401 accggttcat taggcgatgt aatgaaagag tctattcaag cggcaatggt ggttgtacgc
    11461 tctcgtgcag aaaaattagg cattgctgaa gacttccacg aaaaacggga tattcacgtt
    11521 cacgtgccgg acggtgcaac accgaaagat ggtccgagtg cagggattgc aatgtgtact
    11581 gcattaattt ccagcctaac aggtaaccct gtgcgtaaag aagtagcgat gaccggtgaa
    11641 atcagcttgc gtggcaaagt attgccaatc ggcggcttaa aagagaaatt actggcagcc
    11701 catcgtggtg gtattacgac ggtgattatt ccgaaagaaa atgaaaaaga cttagaggaa
    11761 attccggaaa atgcaaaagc agcgttggac attcacgcag tagaaaccat tgacgaggta
    11821 ctagctctgg cactagaaaa cccgccaatg gggattgaaa tcttgtcaaa agccgagatc
    11881 aaggtgaaaa aatcgcgagc taaagccaca gtgcaataaa gtgcaataat ctaaaacaac
    11941 ataaggtgcg cagatcacaa tcacgcacct tattttttat ctgaaagttt tatctacaag
    12001 cggtcggttt tgctctattt tttgcaaaaa tttagcaaaa tctaaccgct tgttatgctg
    12061 aattatcaga caataattgc caaaatgcgt tgatgatcgg ctcttgcaag cgtttttgct
    12121 gaacgcaaat tcccagctca aacggagtga tcggcgaggg taaattcaca taagaaactt
    12181 gattattcat tgggctatgc ttaatcacaa tatccggtag cagtgcgacc ccaaagccta
    12241 gtgcaaccat cggcacaatc gcttcgtgtc cggagacggt agcataaata atcgggtctt
    12301 tgattttttg ctctttaaac caacggtcaa tccgttttcg tacaggtccg ttcaccggca
    12361 aaataaaggg gatatttttc caatctatcg gcgactgttg cagaaattga gtggcttggc
    12421 tcgccacacg aggggcaata atcgaaagtt gaatatcatc aatatagtga aacacaatat
    12481 tattcggcag aaattccggc ttacccgcaa ccgaaatatc tgcagccaat gactgaacct
    12541 gttccaatgc caaagccgga tcaccggtac tcagtttaat atccactttc ggatattttt
    12601 ggcgaaactt tcccaaaatc ggcggtaaat ggctgtaaga agccgttacc gagcagaaca
    12661 ctttcaactc tccttctaaa tcaccttgag caggcgacaa ctggttttgc aactgctgcc
    12721 actctgccca accttgttga gcaaacgcca aaaacctttc ccctgcttca gttaaacgca
    12781 cttgtcgatt atcacgcaga aacaacggct gcccgagctc ctcctctata cgctgaatat
    12841 ggcgggtaag tgtggaagcc gtcatataat tcttctccgc actgcgaata aaactacggc
    12901 tttgggctat atctaggaaa agtttaaggc tttggaagtc catattattt tagccactcc
    12961 ggttttattg cagcaaaatc taaatcattc acgctttttc taaaacggag aatatgggtt
    13021 tcagcctgat tattcggatg ccaatgtata aaattctgtt catcgctact gtaaaaagca
    13081 attatctttt tactcaagcc tgaggcaata tgcacggttg cagtatcagg tgaaattaat
    13141 aagtccgcaa aacgaatcag ttctattgaa tcaaaaacgc ttttggtttt ctcatagaca
    13201 aatatatttt tatacttctt agtaaaggaa cacaattttg ccgttacttc aggaaaagta
    13261 agtaagacta tcggtttatc actatgctgt tttaagcaac tcagcagttc attaattttc
    13321 tgctcactaa aacgtcttgc cgaacctgca ccaaaaagat ttaacgcaat ataatttcta
    13381 atttggtttt catataaata atgctttatg gattggttgc ttgtggcatt tagtggcaca
    13441 tcatactgag tttctatatt ttcaaacccg attaaagcta acgcatcttg ataaatctct
    13501 gaaaaatgct gttttctatc ctctacattt agatcaaata atcgatattt ttgtttctga
    13561 taacctacat aatgtttggc tgatatagtg cgtaaaaata agagatcacg attccgtaaa
    13621 gtaacagtcg gatcgattac aacatcatac tgctcttttg caagttgttt gccaccacga
    13681 atataatcag gaatactttt tgtcttaacc aaatagagct tatcaatata aggattattc
    13741 tcaaataaat atcggttttt ctgtgagcaa accacgccta tttgcgtttg gggagactgt
    13801 ttttttattt cacggaagac aaaggaacta acgacataat caccaatttt accatcgtga
    13861 cgaagaaata gaattttatt gggaatttcc gataatatca gatcagtatt t
//
LOCUS       DANLXQ010000057        13756 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.057, whole genome shotgun sequence.
ACCESSION   DANLXQ010000057 DANLXQ010000000
VERSION     DANLXQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13756
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.057"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(27..743)
                     /gene="rph"
                     /locus_tag="PXD94_001289"
     CDS             complement(27..743)
                     /gene="rph"
                     /locus_tag="PXD94_001289"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL6286158.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVANGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            1024..1887
                     /locus_tag="PXD94_001290"
     CDS             1024..1887
                     /locus_tag="PXD94_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL6286159.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            1896..2786
                     /locus_tag="PXD94_001291"
     CDS             1896..2786
                     /locus_tag="PXD94_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HDL6286160.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDTPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(2818..3078)
                     /locus_tag="PXD94_001292"
     CDS             complement(2818..3078)
                     /locus_tag="PXD94_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL6286161.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            3308..3727
                     /gene="rbsD"
                     /locus_tag="PXD94_001293"
     CDS             3308..3727
                     /gene="rbsD"
                     /locus_tag="PXD94_001293"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL6286162.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQTQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            3737..5233
                     /gene="rbsA"
                     /locus_tag="PXD94_001294"
     CDS             3737..5233
                     /gene="rbsA"
                     /locus_tag="PXD94_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL6286163.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLTIAENIFL
                     GRESKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTSLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAAAIGKSTGLN"
     gene            5243..6211
                     /gene="rbsC"
                     /locus_tag="PXD94_001295"
     CDS             5243..6211
                     /gene="rbsC"
                     /locus_tag="PXD94_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL6286164.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVSVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAGLIVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTL
                     VGALIIGFLNNALNLLDISSYYQMIAKALVILAAVLADNYLGTKKV"
     gene            6234..7112
                     /gene="rbsB"
                     /locus_tag="PXD94_001296"
     CDS             6234..7112
                     /gene="rbsB"
                     /locus_tag="PXD94_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL6286165.1"
                     /translation="MKKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKD
                     GAQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINPTDSEAVGNAVAIAN
                     RNKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSA
                     ARERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMAL
                     GALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKG
                     EKVEAKIPVDLKVISK"
     gene            7233..8162
                     /gene="rbsK"
                     /locus_tag="PXD94_001297"
     CDS             7233..8162
                     /gene="rbsK"
                     /locus_tag="PXD94_001297"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL6286166.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            8176..9186
                     /locus_tag="PXD94_001298"
     CDS             8176..9186
                     /locus_tag="PXD94_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL6286167.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     RLQHHLQMLMQRKVDGLLLMCGDTRLNADESLNIPLPLVVLDWWFTELNADKIFENSI
                     SGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIPLNPNWVFESHF
                     DFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQDISIIGYDDIEL
                     AKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELVLRHSIQTINKN
                     AE"
     gene            complement(9222..9851)
                     /locus_tag="PXD94_001299"
     CDS             complement(9222..9851)
                     /locus_tag="PXD94_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL6286168.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENDPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            10049..12208
                     /gene="rlmKL"
                     /locus_tag="PXD94_001300"
     CDS             10049..12208
                     /gene="rlmKL"
                     /locus_tag="PXD94_001300"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL6286169.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
     gene            12385..12864
                     /gene="trmL"
                     /locus_tag="PXD94_001301"
     CDS             12385..12864
                     /gene="trmL"
                     /locus_tag="PXD94_001301"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL6286170.1"
                     /translation="MLDIVLYEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKL
                     RRSGLDYHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPE
                     SRGIPKPLLAEMPMSQKIRIPMCKDSRSMNLSNSVAVVVYEAWRQFGYQNAVAAQSI"
     gene            complement(12958..>13756)
                     /locus_tag="PXD94_001302"
     CDS             complement(12958..>13756)
                     /locus_tag="PXD94_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL6286171.1"
                     /translation="EQKSVRSHYDEEKDKWYFSIIDVIAILTESLDPSAYWRKLKQRL
                     KAEGNETVTNCHGLKMPSADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQWLAKVGY
                     ERLQEINDPSQSLDRARENWQKMGRSEKWIQQRMMGQETRNKLTDYWKSSGVEQPQDF
                     AFLTNVIHQEWSGLSVQKHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQIAESMQA
                     VGVDENAQAGKQGGNIAKNARQELENKTGKSVVTGENFLPPAKNEEE"
BASE COUNT         3966 a         2705 c         3199 g         3886 t
ORIGIN      
        1 aaaagaaaat caaaaagaga agtgaattat tcagctaacg ctttacgttg agcttcaact
       61 agctggctaa tgccttgctt cgctaaatct agcaatgtca gtaattcttg gtgcgagaac
      121 ggttcgcctt ctgccgtgcc ttgtacttcc actaaacgac catcttcgac catcaccacg
      181 ttcatatcag tttcagcgtt ggaatcttcc agatattcta aatcgcacac cgcttcgcct
      241 gctacaatac cgacagaaat tgctgcgact aaacctttca ttgggttagt ttttagtgtt
      301 ccattagcta ctaacttatt cattgcatcg tacaatgcta cgcacgcacc ggtaattgag
      361 gcggtgcgag tgcctccatc ggcttgaata acatcacaat ctacggtaac ggtacgttcg
      421 cctaaggctt ttaaatctac caccgcacgc agagaacggg caatcaagcg ttggatttcc
      481 attgtgcgtc cgccttgttt gccttttgcc gcttcacgct gagtgcgaga gtgggtggct
      541 cgtggcaaca taccatattc tgcggtaacc caaccttgtt gctgaccttt taaaaagcgt
      601 ggcacgcttt cttctaccgt agcgttacac aatactttgg tttcgccaaa ctcaattaat
      661 accgagcctt cggcatagcg agtgtagtta cgggtaattt taatttctct aacttggttg
      721 tttgctcgat tatttggacg cattttcttt tccttttgct aaaattttta ccattttagc
      781 aaagaacaca gtaaaatgtg atttttcttt agcaaagaat ttacatagcc ctcgcccctt
      841 gtgggagagg gacggatttt tcttcgttca gaagaaaaat cagggagagg ggtaagcggt
      901 tatttttatc tcaaatttta caaattcctc cctctctctg aaaaatattt ctgaacgaaa
      961 tatttttcgg tctctctccc tcaaggggag agagctttac ttataaaaac acaaggaata
     1021 ccaatgattt atagtatgac agcatttgcc catttagaaa tgaaaaaaga gtggggcaat
     1081 gcggtgtggg aaattcgctc ggtaaatcaa cgctttttag aaacctattt tcgtttgccg
     1141 gagcagtttc gcaacctaga aatgaccttg cgtgagcgtt tacgttctgc actgacacga
     1201 ggcaaagtag agtgtagctt acgcattgat ttaagcaaaa cccaaaacag tgaaattgcg
     1261 ttaaacaaag aatatgccgc acaggtggtt caatcattaa aatggttaaa agaaaccgca
     1321 aatgaaggcg aaatcaacct tgttgatgtg ctacgtttcc cgggcgtggt ggataatgaa
     1381 agccaagatt tagaccaaat tgctcaagat ttattagccg gctttgaaca aattctggca
     1441 gattttatcg caatgcgagc cagagagggc gagaacgtgc aagccctgat cttacaacgt
     1501 ttagaagcaa tttcagttga ggctgcaaag gtacaagccc aaatgccgga aattctgcaa
     1561 tggcagaaag atcgtttaca acaacggttc gatgaactga atttacagct tgatccacaa
     1621 cgcttagaac aagaaatggt gttacttgcc caacgtgttg atgtggcgga ggaattagac
     1681 cgcttgcaat tacacgttaa agaaaccacc aatatcctga aaaaaggcgg tgcggttggg
     1741 cgtaaactgg attttatgat gcaagaacta aaccgtgaat ccaacacgct cgcctcaaaa
     1801 tcgattaatg cggacgtgac caattcggcg gtggagctga aagtgttgat cgaacagatg
     1861 cgtgaacaaa ttcagaattt agaataggac agattatggc aatgaatttc aagcaagtga
     1921 gccagagcta tccaaatctc tgcgtgccgc tttctcagta ttgtttaatt gaaatcgcag
     1981 gggtagatgc tgagaaatat cttcaagggc aattaacctg cgatgtggca aaaatttcgg
     2041 tgggcgaaca tacgctaacc agccattgtg atccgaaagg caaaatgagt gcgttactgc
     2101 gtttgtatcg tgcagaaaat gagaaattta tggcgatcat tcaccgcagc ttattgcctg
     2161 aagccctgac gcaacttaaa aaatatgcgg ttttttcaaa agtgaccttc acggaattag
     2221 acaccccact ttatggtatg gcaggtgaag cagcatttgc aaaattaagc gaaaatatga
     2281 ccgctttgcg tttaaccaca ggacagcctc gggccattgt ttggggcgaa gagcttgaaa
     2341 cgactgccga tgaacagctc tggacattaa tggatattca agacggcatt cctgttctct
     2401 tgcaacaaaa ccagtttgag ctgattccac aggcaaccaa tttacaggca cttgaatctg
     2461 cgatttcatt caccaaaggt tgctacatcg ggcaagaaac ggtagcgaga gcgaaatatc
     2521 gtggggcgaa taaacgtgct ttatttaccc ttgccggcaa ctttgaaagc gaaatcagct
     2581 taccggaagt tgcttctgcg attgaaatgc aactgggcga aaattggcga gcaaccggca
     2641 ctgtgttgac cagtgcgact gatgaaaata ccctgtgggt gcaagtggtg ctgaataaag
     2701 atgttgaacc taacaccaag tttagagcca atggtgtagc gttggaaatg gtcgagttac
     2761 cttatagttt ggcagaaaat gagtaaaacg aacggtcatc agggtatgac cgttttttta
     2821 tatctgatca gggtgatgaa tcatcacaaa acgttcccac aactgctctt cggtttcgat
     2881 attatcagga tctttgataa tgcagttggt aatcgggcaa actttctggc aagtcggctt
     2941 ttcataatgc ccgacacatt cggtacataa caccggatcg atgatataaa tatcatcgcc
     3001 caccgaaatc gcctcgttcg ggcattccgg caagcacata tcacagttgg tacatttgtg
     3061 ggtaattaat aaagccatag tttatattga gaaaataaaa attaaacaag cggtcgattt
     3121 tgcaaaaaaa tttgcaaatc ttaccgcttg tcactaaatg ctacctatta taaagcaaaa
     3181 taatttgaac tacttcacat ttttgcaatt tggttgttta tccgattaag aaaatttttt
     3241 atgatgccat cgaaacgttt cgatggagta ttttccaacg tgatggagcg aaaataaagg
     3301 ggttagtatg aaaaaaacgc taaatctaaa tgcacaactt tcgcacagta ttgcgacact
     3361 cgggcatacc gatagtttaa cggtttgcga tgccggtttg ccgattccaa acacaataga
     3421 gcggatcgat ttggctttaa ccgccggtgt gccaagtttt ttacaaacat ttgatgtagt
     3481 agttagtgaa ttatttgttg aacgagtgct acttgctgaa gaaattaaac ataaaaatcc
     3541 gcaagtgtta gcagctcttt tggagcgttt aacccaatta gaacaaactc aacagaataa
     3601 aattcaaatt gattatgtga accacgaaat ctttaaagag cagacacatt acagtaaagc
     3661 tgttgtgcgt tcgggtgagt gttcaccgta tgccaatatt attttatatt cgggcgtgcc
     3721 gttttagggt ggaatgatgg aaaccttatt aaaaatgagc gggatagata aagaatttcc
     3781 cggcgtgaaa gccttgagca atgcgtgctt atctgtttac ggtgggcgag taatggcatt
     3841 aatgggcgaa aatggggcag gtaaatccac cttgatgaaa gtattgacgg ggatttatag
     3901 caaagatgcc ggctctatta gctatttagg caaagaagtt acctttaaag gcccgaaaga
     3961 ttcacaagaa gccggtatta gcattattca ccaagagctg aatttagtcg gtaatttaac
     4021 cattgctgaa aatatttttt tgggccgtga gtccaaaacc gcttggggag ctattgattg
     4081 gaaaaaaatg tatgccgagg cggataaact gcttgcccga cttggggtaa aacacagtag
     4141 ccatcagctt tgtgcggagt tatccattgg cgagcagcaa atggtggaaa ttgcgaaagc
     4201 cctgagtttt gagtctcaag taattattat ggacgaaccg accgatgctt taaccgatac
     4261 agaaacagca tcgttgttta aagttattcg agagttaaaa gcggaaaatc gaggcattgt
     4321 ttatatttcc catcgtttaa aagagatttt tgaaatttgt gatgatgtga cggtgctaag
     4381 agacgggcaa tttatcggcg agcgagtaat tgccgatatt gatgaagatc agttaattga
     4441 aatgatggtt ggtcgccgtt tggaagagca atatccgcat ttacaccaag agcgtggcga
     4501 gttattactt gaagtgaaga atttaagtgg aagtggggtg aataatgtgt cattccaatt
     4561 acacaaaggt gaaattgtgg gtatttccgg cttaatgggg gcagggcgta cagaattaat
     4621 gaaggtgctt tatggtgcat tgccgaaaac agccggctcg atagagttaa atggaaaagc
     4681 gatttccaac cattgtccgc aagatgggtt gaataacggc attgtgtata tttcagaaga
     4741 tcgtaaaggc gatggtctga ttttgggaat gtcggtaaaa gaaaatatgt ctttgacctc
     4801 tctcgatcat ttctccaaag ccggcagtat tcgccacgat gcagaaaaat tggcggtcga
     4861 tgattttatt gagatgttta acattaaaac cccaagtcgg gagcaacaaa tcgggctgct
     4921 ttccggtggt aatcagcaaa aagtggcgat cgctaaaggg ttgatgaccc gcccgaatgt
     4981 gctgatttta gacgagccga cacggggtgt tgatgtgggg gcgaaaaaag agatctatca
     5041 attaattaat gaatttaaaa aagatgggct tagtattttg atggtttcgt ccgatatgcc
     5101 ggaagtgttg ggaatgagcg accgcatttt agtgatgcac gaagggcaaa tcagtgcaga
     5161 attttcacga gcagaagcga cacaagaaaa gctactggca gcagctattg gcaaaagtac
     5221 aggattaaat taaggtgtgt ttatggcaac tcaaacaaag cagtttcaac ttggcagatt
     5281 tttaatcgag caacgttctt ttgtagcgtt atttatttta attgtcagtg tttcaatgat
     5341 taaccctgat tttttcagcg tggataatat tttaaatatt ctacgccaaa cctcggtgaa
     5401 tgcgattatt gcggtcggaa tgacctttgt gattttaatt gcaggcattg atttatcggt
     5461 cggttcggtt ttagccttaa ccggtgcggt tgcagcgacc ttagtcggtt ctgaattgcc
     5521 gattttgttg gtgattccgc tggttttatt attagggacg gcatttggtg gtattagtgg
     5581 ggcgattgtt gctaaaggta aagtgcaggc atttattgcg actttggtca caatgacctt
     5641 attgcgtggg attacgatgg tttataccga tggcagaccg attagcaccg gcttttctga
     5701 ccaagcagac gcctttgcgt ttattgggac aggctattta tttggtattc ctgtgccgat
     5761 ttggattatg gcggtagtgt ttgggttagc ttggtatatt ttgaaacaca ccccgattgg
     5821 gcgttacatt tacgctttag gtggcaacga atcggcaacc cagctttcag gcatcaatgt
     5881 gaataaaatt aagatcttcg tatttgccgt gagtggtttt ttatctgctc ttgccggctt
     5941 aattgtgact tcacgtctct cttctgctca gccaacggca ggtgtgtcct atgagttaga
     6001 cgcaattgca gcggtagttg ttggtggaac cagtctaatg ggcggtaaag ggcgtgtaat
     6061 gggaacgctt gtcggggcat taattatcgg tttcttaaac aatgccctta atttattgga
     6121 tatttcttca tattatcaga tgattgctaa agcgttggtt attttagcag ctgtattagc
     6181 tgataattat ctaggcacta aaaaagttta atctatcttt aaaggagatt tctatgaaaa
     6241 aattaactac gattgcatca gcggttatgt taagtttatc ggttgcagcc actgcttcgg
     6301 caaaagagac tatcgctctt gcagtatcga ctttagataa cccattcttt gtgagcctta
     6361 aagacggtgc acagaaaaaa gcagatgaat taggctacaa attagtggtg cttgattccc
     6421 aaaatgaccc ggctaaagag ctttccaatg tggaagactt aacggtgcgt ggggcaaaag
     6481 tgttattgat taacccaacc gattcggaag cggtcggaaa tgcggtggct attgcaaatc
     6541 gcaacaaaat tccggttatt acacttgacc gtggtgcagc aaaaggcgaa gtggttagcc
     6601 atatcgcttc cgataacgtt gccggcggca aaatggcggg agatttcatt gctcagaaat
     6661 taggctcagg ggcgaaagtg attcaattag aaggcattgc cggcacttcc gccgctcgtg
     6721 aacgtggtga aggctttaaa caagcgattg aagcacataa attcaatgta ttagccagtc
     6781 aaccggctga ttttgaccgc actaaaggct taaatgtgac tgaaaacttg ctctcaagca
     6841 aaggggacgt acaagctatt tttgctcaaa atgatgaaat ggcactagga gctttgcgtg
     6901 cagtgagttc agcgaagaaa gaggttgtaa ttgtgggctt tgacggcaca gatgatggcg
     6961 taaaagccgt tgaaagcggt aaacttgccg caacgattgc tcaacaacct gaactgattg
     7021 gctcattagg cgtagaaact gccgataaag tgctaaaagg cgaaaaagtg gaagcaaaaa
     7081 ttccggttga tttaaaggtt atttcaaaat aattatactt gaagatagcg ttcgtctctg
     7141 acgaacgctt tgaattaata aaatagcact cgtcagagac tagtgctatc agaatcctag
     7201 ttttgttgta ctaggtaatt tgagggctta atatgaaaaa actcaccgtt ctcggtagca
     7261 ttaacgctga tcacgtgatt tccgttccct attttgccaa accgggggaa acgctcaccg
     7321 gacatagctg ccacattgct tatggcggta aaggggcaaa tcaggcggtg gcggcagcac
     7381 gtttgggagc taaggtttct ttcatcggtt gtattggcag tgatgacatc ggcaaagcaa
     7441 tgaaatcagc ctttgagcaa gatggcattg acactcgctc gattaaaacg attccgaatg
     7501 aaatgaccgg cattgcaatg attcaagtgg cggaatcggg cgagaacagt attgtgattt
     7561 cagccggagc gaacggtcat ttggatgaaa cggttgtggc aaagtgtgca gccgaaatca
     7621 cacaggcgga ttatttgcta atgcagctag aaacaccgtt gccggcgatt gtgcaagcag
     7681 caaaattggc tcagcaaaac ggcacgaaag taatattaaa ccctgcgcct gctcgagcgt
     7741 tacccgatga actgttaaac ttactggata tgattacccc aaatgaaacg gaagcggaaa
     7801 tattaaccgg tgtgaaagtt gttgatgaag ccagtgcgat tcaagccgca caagtgtttc
     7861 accaaaaggg catcgaaaaa gtgttgatta cacttggctc aaaaggggta tttgtaagcg
     7921 aaaacggtgt aggcaaaatc gttgcaggct tccgtgttca agcggtggat accactgccg
     7981 caggggatac ttttaacgga gcattagtga ctgcaatgtt agaagataaa ccgctggatc
     8041 aagccattcg ttttgctcac gcagcggcag cgattagtgt gactcgcaaa ggggctcaac
     8101 cttctattcc gacccgtcag gaaacactgg attttctaag ccaacaagcg gtgagttttt
     8161 aggaaaattt tgcaaatggc aacaatgaag gatattgcac ggcttgcaca ggtttccacc
     8221 tcgaccgttt ctcacgttat caacaatagc cgatttgtca gcgaggaaat tcgggaaaga
     8281 gtgcagaggg tggtgaaaga actgaattac acgccttctg cattggctcg tagcctcaaa
     8341 gtgaatgaaa ctaaaaccat cggaatgctg gtaacggcaa ccggcaatcc gttttttgcg
     8401 gaggtgatgg cgggggtgga gcaatattgc cagcaacatc aatataacct gattattgcc
     8461 actaccaacg gtgacaccga gcgtttgcaa caccatttgc aaatgctgat gcaacgaaaa
     8521 gtagatggct tgttactgat gtgtggcgat accagattga atgccgatga gagcttgaat
     8581 attccgctgc cattggtggt gttagactgg tggtttaccg agctcaatgc agataaaatc
     8641 tttgaaaatt caatatccgg cggctatctc gcaacaaaaa ccttaattga agcagggcat
     8701 tctaagattg cgattattac tggaaaccta aaaaaatcat tggcaaaaaa tcgtttagaa
     8761 ggctacaaaa aagcacttgc cgaggcgaat attccactca atccaaactg ggtgttcgaa
     8821 agccattttg attttgccgg cggggttaat ggaatgaatg ctttgcttgc ccaaagcgaa
     8881 aagccgacag cggtatttgc ctgtagcgac accattgctc taggggctta ccaagcaatc
     8941 tggcaacaag gtttacgaat accgcaagat atttcgatta tcggctatga cgatattgaa
     9001 ctggcaaaat acctttcccc accgctgagt accattagcc agcccaaagc ggaactcggg
     9061 cggcttgcgg tggaaacttt gctaaaacga attcgtttta aatccactga ttttcaaacc
     9121 ataatgcttg aacctgaatt agttttaagg cattcaatcc aaacaataaa caaaaacgct
     9181 gaataatgtg aagttattca gcgtttttta tccgatttca attagccttt ttgctgttcc
     9241 aaataaagca cggtggcggc aactcgggaa tgcacgttta atttacgcag taaattgcgg
     9301 atatgcactt ttacggtttc ttcggaaata aagagctgag ccgcaatttg tttgttcgac
     9361 ataccggttg cgagccattg taaaacatcc agttcacgat ctgttaagca acttaaagga
     9421 tcattttctg gctggcggtt gagtaaatgt tggcgaacgg tttcgcttaa taccacttca
     9481 ccggcagccg ctttacggat attatctagt agttccgggg tgtcggtatc ttttaataaa
     9541 tagccgtcca caccagcatc cattaatgca aatacatcgg attgctcgtc tgacactgtt
     9601 aaaacaatga ttctggcatc tataccttga gcgcgcaatg agcgtaaagt atctagccct
     9661 gaaagccctt tcatatttaa atccagaata atcacatccg gctcaagtcg taatgcaagt
     9721 ttgataccgt cggtgccgtt gcttgcttca cccactacac taaattggtg atctaattcc
     9781 aatagttgac gcattccctg acgcattaat gggtggtcgt caatgagtaa aatttgagtt
     9841 ggagctgtca tttatttctc ctttcctttc tcttaatcgg ttaattttgc aattattaat
     9901 tcaaaatagc aagtttaggg atttactaaa tgccgctttt ttgatcttag ctaattttta
     9961 actaaaaaag aaattgcaaa actttgcaag aaaaagaccg cttgttcggc tataattact
    10021 gccaattttt atttcaagtg tgaaaagaat gacaaatcaa acaacctatt ttgccacggc
    10081 agcccgtggt tttgaggaaa tactaaaaac cgaactggag cagatttgtg cggcagaatg
    10141 caaagtcgct caaggcggtg tgcattttac caccacgcaa aaaggggctt atcaggcatt
    10201 attacacagc cgtttagcct ctcggatctt actgccgctc atcagcacca aaatttttag
    10261 tgatagcgat ttatatgctt ccatcgtgtc atttaactgg gcggatttat tcgatccccg
    10321 tgataccttt tacattgatt tcagcggcac taaccgagaa atccgcaaca cccaatttgg
    10381 ggcaatgcga gtgaaagacg gcattgtgga ttactttgaa cgcaaaggtt ttgcccgccc
    10441 aacagtagat aaagataatc cggatgttcg cattcatgtt tatttggatc gtgaaaatgt
    10501 tgtggtttcg cttgatttaa gtggagatgc cttacatatt cggggctatc gggaagaaac
    10561 cggtaaagct ccgttgcgtg aaacattggc ggcagccatt attttgcgtt ccggctggaa
    10621 agtcggcacg ccattggttg atccgatgtg tggttcaggc acgttactga ttgaagcagc
    10681 acaaatggca gcgaatattg cccctcaact tcaccgtaaa cattggggct ttaatgcgtg
    10741 gaaagggcat caacaagcga tgtggaaagc cgtgttagat gaggcattcc gaaatgttga
    10801 attgggtgcg gtaggggaga attgcaattc gtccctacaa aaaatgttct tcggttttga
    10861 tctcgatcat cgtgtgctga ctaaagcaaa gcaaaatgcg aaaaatgcag gggtagatca
    10921 tctcattcaa tggcaacaag gtgatgtggc tgcgttaaaa aatccgattc cggaaaaaat
    10981 cggcacggtg gtgtgtaacc ccccttatgg tgaacgttta ggcacaacgc cggcgttaat
    11041 tgctctttat tcggtgttcg ggcaacgcct aaaacagcaa tttgccggtt ggaatgcctc
    11101 aatttttagc ggcgagcctg aattgttaaa ttgcctgcgt ttacgttcag cccgtcaatt
    11161 caaagcgaaa aacggtccgt tggattgtgt gcagaaaaat tactccatca gcgagcataa
    11221 caagcggtca gatttagatg aaaatttaca atttgaacag aatgctcaag tcgctccgga
    11281 ttttgcaaat cgcttaacca aaaatatcaa aaagatcgaa aaatgggcga aacaacaagg
    11341 cattgatgcc taccgtttat acgatgccga tctgcctgaa tataatttag cggtggacag
    11401 atatggcgac cacattgttg tgcaggaata ccaagccccg aaaaatattg atgagcaaaa
    11461 agcccgccaa cgcttgttag atgcagtctc tgctacgctt tatgtgacag gtgttgaaac
    11521 taataaatta gtgttgaaag ttcgccaaaa acaaaaaggc acgaatcagt acgaaaaact
    11581 tgccaataaa ggcgagtatt tctatgtgaa cgagtatggg gcgaaactgt gggtgaattt
    11641 aaccgattac ttggatacgg gtttgttcct agaccatcgc ttaacccgta aaatggtggg
    11701 cgaaatggca aaaggcaaaa cattcctgaa tctgtttgcc tacacgggtt cagctacggt
    11761 tcacgctgct cttaatgggg caaaatcgac cactacagtg gatatgtcga acacctattt
    11821 gaactgggcg gagcaaaatt tagagctgaa taatttatca cttcgccatc atcgtttgtt
    11881 ccaagccgat tgcttgcaat ggctggcaga atgccgtgag cgatttgagc tgatttttgt
    11941 cgatccgccg actttctcca actcaaaacg aatggaaaac agttgggacg tgcaacgaga
    12001 ccatattgag ttaatgaaac agcttaaacg tattttaacc gctgatggta ccattgtgtt
    12061 ctcgaacaat aagcgtggct ttaaaatgga ttttgacggt ttggcagcat taggcttaac
    12121 ggcggaaaat atttcgcata aaaccttacc gttggatttt gaacgcaatc cgcaaatcca
    12181 taattgttgg attgtgaaga atgtatagat aataccctcg acccttgtgg gagagggata
    12241 gatttttctt cgttcagaag aaaaatcagg gagagggtaa gaagttactg atgaaagagt
    12301 tatttccccc tctctctgaa aaatatttct gaacgaaata tttttcggtc tctctcccac
    12361 aaggggagag agtaaggcta aaaaatgtta gatattgttt tatacgaacc tgaaattccg
    12421 caaaacagcg gaaatattat ccgcctttgt gccaactgtg gctttcgttt gcatatgatt
    12481 gaaccgttcg gctttggttg ggacgataaa aagctccgcc gttcggggct ggattatcac
    12541 gaatttgtgg atattcaaaa atataaaaat tttgaagatt tccttgagcg tgctaagcct
    12601 aaacgcttgt tcgccttaac caccagaggg gagcagaacc acagtgatgt gaaatatgaa
    12661 cttggcgatt ttttaatgtt cgggccggaa agcagaggca ttccaaagcc attattagcg
    12721 gaaatgccga tgtcgcaaaa aatccgcatt ccgatgtgca aagacagccg cagtatgaat
    12781 ttatctaact cggtggcggt ggttgtgtat gaggcgtggc gacaattcgg ctaccaaaat
    12841 gcggtagcgg cacaatctat ttaacttgca agcggtgtga ttttggctat tttttgcaag
    12901 tttgtggtca aggttggggg aggctcgacc ttgactaatt aagaacatct attgtgtcta
    12961 ttcttcttca tttttagctg gaggtaaaaa attctctccg gtaacaactg atttacctgt
    13021 tttattctcc aattcctgac gggcattttt ggcaatattg ccaccttgtt ttcctgcttg
    13081 ggcattttca tcaaccccaa ctgcctgcat actttctgca atttgccgac tggaaagttc
    13141 agcaagtgct gtaaataaca attcagcttc gctcatatga tctcgcagat tttggctttt
    13201 taaaccttta agcgatttat gcttttgtac agatagtccg ctccattctt gatgaatcac
    13261 attggttaaa aatgcaaaat cttgtggctg ttcaacccct gacgatttcc aataatcggt
    13321 cagtttattt cgggtttctt gtcccatcat tctttgctga atccattttt cacttcgtcc
    13381 catcttctgc caattctccc ttgctcgatc tagactttga cttggatcgt ttatttcctg
    13441 taagcgttca taacctactt ttgctaacca ttgtttgaag ggctctgctt tcggactagg
    13501 gatagattga ataagtcgga aaagttgttc ggtatctgct acatcagtca aacgcatttt
    13561 tccgtcagca gagggcattt tcaacccgtg acaatttgtc acggtttcat tgccttctgc
    13621 ctttagcctc tgttttagct tacgccaata agcagaggga tctaggcttt cggttaaaat
    13681 agcaatcaca tcaataatag aaaaatacca tttatctttt tcttcatcgt aatgactgcg
    13741 tacagatttc tgttca
//
LOCUS       DANLXQ010000058        13474 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.058, whole genome shotgun sequence.
ACCESSION   DANLXQ010000058 DANLXQ010000000
VERSION     DANLXQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13474
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.058"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            13..408
                     /locus_tag="PXD94_001303"
     CDS             13..408
                     /locus_tag="PXD94_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6286172.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
     gene            complement(618..2777)
                     /locus_tag="PXD94_001304"
     CDS             complement(618..2777)
                     /locus_tag="PXD94_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL6286173.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHETQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARLSSQKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPERYVMASVEFKF"
     gene            2884..3750
                     /locus_tag="PXD94_001305"
     CDS             2884..3750
                     /locus_tag="PXD94_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6286174.1"
                     /translation="MEYKMRTLLFSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGK
                     ADKLVGVDTTSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQI
                     KASGIPLLHLGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHS
                     TDKEVLVLIDTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADV
                     ILLASRNQAKESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYA
                     HQILTTIQPCLK"
     gene            3761..4747
                     /locus_tag="PXD94_001306"
     CDS             3761..4747
                     /locus_tag="PXD94_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6286175.1"
                     /translation="MKLHLTLIFTLIISLVICTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGALFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYVADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            4747..5553
                     /locus_tag="PXD94_001307"
     CDS             4747..5553
                     /locus_tag="PXD94_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6286176.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(5574..6212)
                     /gene="purN"
                     /locus_tag="PXD94_001308"
     CDS             complement(5574..6212)
                     /gene="purN"
                     /locus_tag="PXD94_001308"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL6286177.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(6319..8476)
                     /locus_tag="PXD94_001309"
                     /pseudo
     CDS             complement(6319..8476)
                     /locus_tag="PXD94_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(8530..9366)
                     /locus_tag="PXD94_001310"
     CDS             complement(8530..9366)
                     /locus_tag="PXD94_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL6286178.1"
                     /translation="MLPRISSFLASLFMAFFLLGCSVSTQSLNEQNVDSIGQIIDLNT
                     NQTISFTELLSRLSSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVD
                     QQPAIDRIQFNRPVNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKE
                     VEILLNGAEPLKGSVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKL
                     VKNALSTSLLIAGNHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWV
                     TQ"
     gene            complement(9524..10786)
                     /locus_tag="PXD94_001311"
     CDS             complement(9524..10786)
                     /locus_tag="PXD94_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL6286179.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPVYA"
     gene            complement(11062..11283)
                     /locus_tag="PXD94_001312"
     CDS             complement(11062..11283)
                     /locus_tag="PXD94_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286180.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
     gene            complement(11371..12402)
                     /locus_tag="PXD94_001313"
     CDS             complement(11371..12402)
                     /locus_tag="PXD94_001313"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL6286181.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTDKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            complement(12490..13467)
                     /locus_tag="PXD94_001314"
     CDS             complement(12490..13467)
                     /locus_tag="PXD94_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL6286182.1"
                     /translation="MTTNFKCQVHTIEFNEDYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDENADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
BASE COUNT         3903 a         2876 c         2555 g         4140 t
ORIGIN      
        1 aaaggaaaat atatgaaaaa attgatgata ttcgcaacaa ctgccatgat tgtaagcaat
       61 ttagcccatg cagcaggtga acaaagtgat gcaagagaag cccacgaaag caccgtaaaa
      121 acctcaactg ttaagtatag ctgccaaaac ggcaaaaaat taagcgtgaa atacggcttc
      181 aataaacaag gcattccaac ctatgctgag gcaaaattaa gcggtaaaaa acgttttatg
      241 ccgatcaacc tatacacgac agatgcaacc ggcactaact ttggtgacga aaacaatttc
      301 agcctttacg gtgatccaat ggaatttacc aaccaccgta aagcatcagt aaatatccaa
      361 agccccgcaa gtgaaatttt atataaaggt tgcacacctc aaaaatagat aaaaagtaaa
      421 atgagtccgc tctgataagt ggctttttaa tagctgttta ttgcagatag gaaagcattt
      481 tgtttttatc tactatccag aaacaagcgg ttaaatttgt ttaatttttt acaaaaaatg
      541 ggccaaaatt aaccgcttgt ttattactat aaagaaataa gtacgtaaaa attaactctt
      601 acatttaatg cttatctcta aaatttaaac tccactgatg ccattacata gcgctctgga
      661 gcaaggtaac gatttaagcc ttcgccagtt atactgttgg caactccagg actagttata
      721 ccaatagttc ttaaactatc ccaagtccaa tattttttat taaagagatt ataaacccca
      781 actttaacaa tcgctctttc tccaaacttt ttgtgtgcaa ctaagtcaaa tacggtggct
      841 gaattactta agtatgggta agtggttaat gatttatcgt aacttaaata cccagtattg
      901 gctaaaatag catactgttg tgcatatttt gcttttttgc cttgtgtgta tgtgccgttt
      961 aggtgtatcg cccagtcatt gttcacagct cgataaccta tgccgaacaa tgcttttaac
     1021 ggttgaaccg tgagtaagtc ggtaccatca ttacgtttgc cacgagcata accaaatcca
     1081 ccattaagct caaagccttg tggaattgca ttccacgctg tgtttaagtc taaagttccg
     1141 ttaatatcaa cacctttgat tttagctcgg ttaacatttt gcgattggta aatattgtta
     1201 ccaatagtgg caagatcaat cagatttcta tagtgggtat ggtaagcggc tacgctcaag
     1261 ctacctaatg aaccattacc taccagtgaa atttcattac ctaagcttgt ttccggtttt
     1321 aagttagtat ttgctaagaa agtttggctt gctccacgcc cttgaacttc acggttaaag
     1381 aacatttcac ttgcggtagg tgttctaaaa ccacggctga gtttatagcc aatttgccat
     1441 acaggagaga gctgataatt taaaccaata tctcccgccc aattgttaaa ggttttatct
     1501 gacaaactat tatcatcaga atgtaatttc tgactgctca atcgagcatt taaatgagca
     1561 gatagatttt gtgtcagtaa aatagagtct cccatcaaaa tatggaaaga cttggttttt
     1621 gccggagact gcatattttg atcggttggc gtaatacctg ctgtataggt ttgcatatca
     1681 aaactgaaat cagatacacc atagcctaat tccgttgaca atttatgttc ggtattacct
     1741 agatcaacag gcttgaatgt aacacctaag ctaccttcaa catttttagt attaaaagcc
     1801 cgtttggtat tatctgtttt tgtaccacgg gcaatagtga tttgatcgtt aatacctgaa
     1861 atttcagtat cctgtttggt taccgcaact ttaatatttt caagaatact gttttttgga
     1921 gtgtataaat aagtaacgcc ataagtttca atttcctgca catccaaaaa atcacgtaat
     1981 gtgacggtag aataagaaag ctcttgaccg gcgtttttac tgcgttgctt agaagttttt
     2041 aattcaaggc gattttcatc attccatttt agccctaatt ttgccaagta gctgtattga
     2101 ttatttttgt aaggatttgg taaaccacga ccacttccca taatatccgg acctttagac
     2161 gttgtttgtg tttcgtgtcc ttgacggtgg ctatacataa ccaaagcatc cgcatattga
     2221 ccatccgcag caatacctgc cgtctcaata ttctctttat tttgaccgtt ataggtatat
     2281 ttcaacatcc caccaaattg gcgacctttc attaaaatat cacttacggt tttagtttta
     2341 tagttcacag agccgcctaa tgccccattg cctacttttg tgcttgagcc tttttcaatg
     2401 gttacatcgc tcattaattc agtatcgatt tcagggcgag atttattgta gtaactatag
     2461 cgactaaagg tggcatagtt atttgatgct gcttgcgata tgccatcaac tgttactgcc
     2521 acttgattac cttcaacacc acggatatta aacccttgtg ggccaaagcg gcctgtttgc
     2581 ggaacgccca tatcagtact ataacgtact aaatcttcat tgctcatcac cgcttgctgt
     2641 ttaagtaggt tctcctgctg ctcatttact tcaatagtat ctagttcaat gctactaatc
     2701 tttgtttctt gagctaagga gagttgagaa ataaagggag taatcgctat tgctaataca
     2761 gtttgcttta tttgcatatt ttagatcctc tagttagttt aatccgttta atgttaacac
     2821 tatatcattt taattgaaaa ttgaaataat aataattatt atttaagttt agataaaaat
     2881 caaatggagt ataaaatgag aaccttactt ttttctttca ttgccctatc aatgtgttta
     2941 aataccacaa aggcggcaga aaaaattgtt tcaactgcgg gctatgcttc cgaaattgtc
     3001 gcagcactag gaaaagctga taagttagtc ggtgtggata ccacttcagt taaacctcaa
     3061 acgataatgg agaaaaaacc taaaatcggt tatcgtcgcc agctttctgc tgaaggtatt
     3121 ctctctttaa ccccagattt gattatttta gcccctgatg ccggccctaa cgcagttatt
     3181 gagcaaatta aagccagcgg tattccatta cttcacttag gagagaaacc gagcttagca
     3241 gggattcgtg aggatattca gcaaattgcc aaggcgattg gtgcagaatc agaggcaaaa
     3301 caattaacag aaaccattct ggcagatgaa aaaaaattag ccgacattcg tagccaacat
     3361 tctactgaca aagaagtctt agtgctgatt gacactgcat cgcaaggtat ctttgggcta
     3421 gggaaaaaga gtgtgggtga tcatttctta accttgttaa atctaaacaa tagtttcaat
     3481 gctgagggca ataagccact cgccaatgag gctttagctg caagcaatgc cgatgttatt
     3541 ttattagcct cccgtaatca ggcaaaagaa tctgcaacga tcagtaaatt agcagaatcc
     3601 agcccacaat acgcccaact cacaaataca caagcgggca aaaaaggctg tgtttttgca
     3661 atcaatattt tagatgcctt gggctttggg ccttacactg ctcaatatgc acaccaaatt
     3721 ctcaccacta ttcaaccttg tttaaaataa cggatacgct ttgaaactgc atttaacctt
     3781 aattttcact ttaattatca gcttagtgat ttgtacccat ttaggggcga tgtcattacc
     3841 cctatccgct tggcactgca acaacgctga atgcgaaagt gcagcctata ttttatggca
     3901 aatccgcctg cctcgattac taggggcatt attcatcggg gcagccctcg ctattagcgg
     3961 agcaacaatg caagggctgt ttcgcaatcc gttagtagat ccggggatta ttggtgtctc
     4021 ttcaggtgct gcattggctg cggcattata tattgtggtg attcagccac tactgccgga
     4081 atcaatcagc tattatgctc tacctattgc cgccttttta gggggctggc tggtgacttt
     4141 tatgctctat ttactttctc aacgtaacgg gcaactacat atcgccatta tgctattaat
     4201 tggtattgct ctagctgcat ttagtggcgc tttaaccggc atactgattt atgttgccga
     4261 tgataaccaa cttcgtagtt taaccttttg gagtatggga tctttagcag gtatgaattg
     4321 gcaaatggtg gcaatgatgg ctgcggttgt cttcatttgt agcccactta tttggcggga
     4381 acaccgtgcg ttaaacgcct taacattagg tgaagaagat gcacaataca ttggttttga
     4441 cattgcaaaa gtcaaacgtc gtcttgtgtt ttacgttgcc ttattaaccg gcacaagcgt
     4501 tgcctgtgtt ggggctatcg gctttgtcgg gctggttatt ccacatttaa tgcgtttact
     4561 gggaggcagt aaccaccgcc atttattact caatagtttg gtttgcggtg caatcttatt
     4621 aatgattgca gatactcttg ccagaaccct agccgccccg gcagaaatcc cgattggtat
     4681 ttttaccgcc ttatttggtg cgccattcct tgcagtaatg gtatggaaaa gtgggaaaca
     4741 tatctaatga gcaatttatt aacttgccaa aatttaagca tcgttcatca acaaaaaaag
     4801 ttgctggaga ttcaagaaat tgcctttgaa caaggaaaat ttaccgccat catcggtcca
     4861 aacggagccg gcaaaacaac cttgctcaaa gccttaagcg gtaataatgc acaaactaac
     4921 agtgccgtat tttatcaaca tcaacgctta acagacttaa acggatatgc tctggctcaa
     4981 aaacgggcaa tgctcagcca acagagccaa attgcctttc ccttaaaagt agaagaattg
     5041 gtacaactcg gaagagagcc atatcgccaa agtgaatttg caaaatttaa tgaaaaaata
     5101 accgcttggg ctatggaaac aatgtcttta accgcattaa aagaaagaag tagcatacaa
     5161 ctttccggtg gagaacaaca tcgagcgcat attgctcgag tgctggctca actcgttcct
     5221 gaaccgaatg ccgatttaag cggaagctgg cttttgcttg atgaacccac caaccatttg
     5281 gatattcatc accaatatca gcttttttcc ttactacaaa agctgaaaca acaaggctta
     5341 actatcattg cgattttgca cgatcctgca ttggcaataa atcaagccga tcatatctta
     5401 atgctaaaaa atggagaaaa atttgctgaa atgtccgcta aagccattgc agaaagttgt
     5461 ttattagatg agctgtatga aatgaaaatg ggctgtcgct attgccctca cagcgggcaa
     5521 tattatcttt gccctgcatt agctgaaagt tagggtgcga tgcaccctat cgtttaatct
     5581 tctgcatatc cacaagcagg cagtagttta ccgtctaaat acgctttgcc gtgctgagaa
     5641 actaatctac ccgaacagaa ccactcaatc accaacggat aatatcgatg ttcttgctca
     5701 accactcgag ccattacatc ttcaatctca tcttcaggat aaatcggcac tttagcttgt
     5761 aataccaccg caccagcatc cacttcttca ttcacgaaat gaatagtcgt gccgtgttca
     5821 ctttccccag cctctatcgc tctttgataa gtgtttagcc ctgggtattt aggtaataag
     5881 gaagggtgaa tattgagtat tttgccagca aaatgttgcg taaattcagg cgttaaaatt
     5941 ttcatatagc cggctaatac aattaactca gctcctaact gctcaatttg ttcggcaatc
     6001 gcaagatcca tttccacatt gctttggtaa tctttacgag aaaatacaaa tgtcgggatt
     6061 cccgcctgtt tagctcgaat cagcccatag gcatctgcct tattgcaaat tacaccgcaa
     6121 atttgaccgc ttgtatccgc agatttttgc gcatcaatca ttgcttgtag gttagaacca
     6181 ttccctgaaa ttaaaaccac aaattttttc atattacaca ccggctgaat attcatcgtt
     6241 ctggatatta tgccttgaat ctcaacatca gtacaggaaa gaaaaagggc gtattggtta
     6301 aaataagcca ctttcaagct agaaacgcac ctctgcccct agtgtgtaag ttcgacctct
     6361 ggcactattt gtgaaacgat aggtgtctgt atcatagtca tactcttctg cggtggaaga
     6421 gttatacgca tttaaggcat caagataatt tcggttcatc acattttgta ccccggcttt
     6481 taaggttaaa tgtttattca ctttgtaatt tgcatataaa tcgataatca ccggaatatt
     6541 cggtaactgc tcgtgaataa tgttgccatc ttcgtctaaa tcaataccga cacgtttcgc
     6601 tttgcctgta tatttaaaaa tgctagacag tgttaatttt ttatccagca atttagcccc
     6661 tagctccaaa gtcgcataat cttttggtag ctgggtataa gcttcagtgc caaatccata
     6721 aagaatagtc gcaccttttg aagtgggtgc tgtgctttca gttcgggtat aactaccatt
     6781 gacaaaaaaa gcatcggaat cgtaagccag ctcaacttcc caacctcgat ttttaacctt
     6841 ttcagggtga ttcacataaa tttggaactg tatagcattg gtagcatcac ttgaatttcg
     6901 acatactgtt ggcccacaac catacaacga gaggttatac aaatagtctt taatgcgtga
     6961 gttaaaatat accgctttta gccccaagat atcattattg agtaatacat tatcttgatg
     7021 aatattaaac ccaatctcat aggttttcgc actttctggt ttcaaaaacg ggttcacact
     7081 caagccattt cgactggtat taaacatttc ttgcacatta ggcgaacggg aggtttgttt
     7141 gtaactgaca aaaggttgaa accaatcagc aacctgaaat gctaaagtgg cagaaggatt
     7201 caacttataa tgttttttat taacatcaaa cccaaaagaa gggaaacaat aatccatatc
     7261 atcgctatcg cattctggct tatatccttt taactgatag cgtgaatagt gaagacttga
     7321 atttaaagtg acaatgccgt atttccattc ggtgtctagg taaagggaat caatattttg
     7381 cttacctgcc ggcccaaaag cggcattgct aatcgctcca tcattattct tactttccgg
     7441 taaatcacgg gaatatttat ttccctgact caaaacggct ggtgttattc aaatcaaaca
     7501 tccacatttt gtttttggtt gaggcatctt ctaatgtcca tagttttgca tcatcgtgga
     7561 aatcttgctt agtttctgtt actgctgtta ggaatttcag atcaatccaa tcagattgag
     7621 gctggtaact ggattctaag gaataggtca cactatcaat attacgcccc actaagctat
     7681 ttttatattg acgtccggac aatttaaact ctgaatcttg atttggctta aaggcaattt
     7741 ttcccatata agattcaggg tgatttttta acccttgcat ataagcagaa tccgttgcca
     7801 ttgtgccatt acctcggcga taattttgac tgatactact tttgctaaaa gccaataaac
     7861 tgccaagaga accactttcc agcacatcaa cttttccggc tagtgcaagc attccgtttt
     7921 ttcccagatc attattgcca taattaaaac ggcttaatac gccgacttta tgttgttcta
     7981 gtagaatatc ttcgacatca atcgttttaa aattagcact accggctaaa gcattcaatc
     8041 cggctgcacc attgttgtag cctttcgcaa tatcaactcg ggttaaaaaa ttttgatcaa
     8101 ttaaagtgct gaattggctc attggcccgt taccatcagt gtggaactta ccacctccgt
     8161 ttgctgatgt accaagctgt gtttgtggca cgccatcaac taaggtattt acccgaccaa
     8221 gaccactcat attacgaata ttcacactca cgcccccttg ccccgggtca atttgggtat
     8281 agccacctgc gacagaacgg acaatactat ctaaactttg cattgcattc acattcattg
     8341 ctcgagaact caccgagccg gctttactga aattctcaaa attagggttt tccacagtgc
     8401 cataaaccgt cacagtatca agctccgcaa tatttttcgc cttttcctct gccaatgcag
     8461 tttgcgatcc tcccatacac aaaaccggaa acaaaatgcg tgtgattata ttaaatttca
     8521 taattctcct tattgagtga cccaaagata atctgcctgt gaagaagtaa tttcttctct
     8581 ctctgtttta agcattagca ccgctacttt ttttgttcgg tcatattccg caatatgcaa
     8641 cggaacgcca agatctttgc gaacgtggtg gttacctgca atcagcaagc tagtcgataa
     8701 cgcatttttt actaattttt ctgccattct gcgatcacgg aattgttgta ctgccaccat
     8761 attttccatc gggtaaggca ttccgtgatt cattgacagc aacagttgtg cgatcttttg
     8821 cttaacttca gaggaagtgg atacagaacc ttttaatggc tccgccccat ttaataaaat
     8881 ttcaacctct ttatctgtta agttagtggc tataacacga ctatttgaac ctaagctctc
     8941 taccactaaa tcttgataca tcttccaatc ccatttttgc cattgaattg cctctcttaa
     9001 atccgtggct gataaattaa ccggtcggtt aaactgaatg cgatcaatcg caggctgctg
     9061 atccacattt aacatctcca acgcaattaa atgtaacttg gctttcttcg taagttgatt
     9121 aaataaatac aattcaattt ggtggtgaat taactgagta tgctcctcac caactaaaac
     9181 gtaatcttga gaagatagac gggaaagtag ttcagtaaat gaaatcgttt gattggtatt
     9241 cagatcaata atttggccga tagagtccac gttttgttca ttaagggatt gcgttgaaac
     9301 cgagcagcct aaaagaaaaa aagccataaa aaggctagct aaaaaagagg aaatacgagg
     9361 taacataaca aaaaataaat taatgagaat tattatcatt atgttatgta aaaaaagaaa
     9421 aatcaatcca ttttcagaaa acagataaag aaaatgattg ataaaacaaa aagcggtaat
     9481 atttctaaaa aatattaccg cttgaaaaag tgtaaagttg agattatgcg tacactggta
     9541 aacgtttaca aatgtctaaa actttcgctt tagtctctgc aatcacctgt tcgtgattat
     9601 ccttaccaat gctatctagc acatcgcaca tccagccggc taatgctgtc acctctgctt
     9661 ctttaaaacc acggcgggtc acagaaggcg tacccacacg aatacctgaa gtcacgaatg
     9721 gtttttgagg gtcgttcggc accgagtttt tattcacggt aatgttcgca gctcctaatg
     9781 cagcatctgc cgctttgccg gttaaacctt gttttacaaa gctaacaagg aataaatggt
     9841 tttccgtacc gtttgaaacc acatcaaaac cacgttgctt aaatacctca accattgctt
     9901 ttgcattttt tacaacttgt tgctgatatg ccttaaattc cggctctaaa gcttctttaa
     9961 aacaaaccgc ttttgccgca ataacgtgca tcaatggtcc accttgacca gcagggaata
    10021 cggcagattg cagtttctta tacatttctt catcgccaga tgcagataaa atcaaaccac
    10081 ctcgagggcc ggctaaggtt ttatgggttg ttgtcgtcac gacgtgagcg tgaggaagcg
    10141 gatttggata aactcccgcc gcaattaagc ctgcaacgtg agccatatcc acaaataaat
    10201 atgccccaac ttcatcggca atctcacgca tttttgccca atccataact tgagaataag
    10261 cagagaaacc agcaacaatc attttcggtt tcacttcttg ggcttgtttg cgtaatgctt
    10321 cataatcaat taagccttca tcagtaatgc cgtattgttc tgctttataa attttaccag
    10381 agaagctaac tgaagcaccg tgggttaaat gcccgccgtg cgctaaactc atacctaaaa
    10441 tagtgtcacc cggattaagc aacgccatat atacggctgc attcgcttgt gagcctgaat
    10501 gcggttgaac gttggcataa tctgccccga acaatgcttt cgcacggtca atcgcaagtt
    10561 gttctacaat atccacatac tcacaacccc catagtaacg tttacccgga taaccttctg
    10621 catatttatt ggttaattgt gagccttgag cctgcattac acgtgggctt gcatagtttt
    10681 cagatgcaat taattcaata tgttcttctt gacggcgatc ttcatcttga atggcttgcc
    10741 ataatactgc atcgtaatca gcgatattca tatcacgttt taacattttt ggactcctaa
    10801 taagcttaag taaacgtttg cgtaagttga tttcacgtta ttttacgcat tttttagttt
    10861 catagatagt attatttata taaaaattaa cttcgagtga aagaaaaaca agcggtcaca
    10921 ttttgtaaaa aatttaccaa atatgaccgc ttagtattat tcattgaatt aatgattatg
    10981 atcttttaaa tgctttgccc attggtatgc agcaaatgaa aatgcaagca aaagaaataa
    11041 tccgattgtc aatccgtcca tttacatctc ctctgctgtt tctcgaaaca atcttgccgt
    11101 tgctaacaaa aaataaacta ctgcaaataa ggtaattgag tgtgtaaatt tcattagcag
    11161 tgaatattct ccatagagaa taacaggaat tgctaaaatt gcaacttttg ccacatcgtc
    11221 cattagtttt gcccaagcct caaaggagct tttcttaatt ggctttttaa atagatttag
    11281 catagtgcct ccgtcatttc ctgttatttt ggaagaaaat ctggaaacga gcaaaaagca
    11341 aagttgatat tttcgatgca gatcgcaaaa ttattttgcg aattccgcac gcaatatcgc
    11401 tgccccttgg ctgagtgcct gtaatttacc ttgtgcgact tggcgtgaaa gtggtgccat
    11461 tccgcagttg gtgcttggat aaagtttatc cgcatcgaca aattgtaatg ctttgcgtaa
    11521 cgtatctgcc acttgttccg gtgtttcgat aatgttggta gccacatcaa tcgctcccag
    11581 cattactttt ttgccacgca ctaattcgat taagtccatt ggcacacggg agttttggca
    11641 ctctaacgag ataatgtcga tttttgattg ttgcagtttc gggaaactct cttcgtattg
    11701 acgccattcg ttgccgaggg tctgtttcca gtcggtattg gctttaatgc cgtagccgta
    11761 gcagatatgc accgccgttt cgcattttaa gccttccgcc gcacgctcta acgccgccac
    11821 gccccagtcg ttcagctcat cgaagaagac gttaaatgcc ggttcgtcaa attgaataat
    11881 atccacccct gccgcttcca actctttggc ttcttcgttg agaattttgg caaattccca
    11941 cgccaatttt tcacggcttt tgtaatgacc gtcatagaga gtgtcgatca ttgtcatcgg
    12001 gccgggtaat gcccatttga ttggtttgtc ggtatgcgca cgcaagaatt tggcatcgtc
    12061 cacaaacacc gatttcgggc gggaaactgc ccccaccacg ctcggcacgc tggcatcgta
    12121 acggttacga atgcgcacga tttcacgctt gctaaaatcc accccttcca aatgctcgat
    12181 aaaggtggtc acaaagtgct ggcgggtctg ttcgccatca ctaacaatat caatccctgc
    12241 cgcaatttgt tcggcaaggg aaacaaggag ggcatcacgt ttggcttgaa gtagttcctc
    12301 gccttcaaat ttccaaggtg accacagttt ttccggctcg gcaagccacg aaggttttgg
    12361 taggctgccg gcggtagagg ttgggagaag aatgttgctc attttgaatt ttcctataaa
    12421 aagtagggtg ggcgtaagcc caccattggt tatatctcat tttatggtgg gcttacgccc
    12481 accctacgtt tattcctccg tcgcccaagc ctctaaaatc gcttggtaac gtttaataaa
    12541 atgctcttcg gtgtatttac cctgtttgat cgccaactgg gtgcgttctt cacggtcata
    12601 ttccactttg gtgacggaat aatcttggta agtcaggctg cctttataga cctgccctgc
    12661 gaccgaattg gcattgtaaa tttccggacg gtaaattttt tggaacgctt ccattgtggc
    12721 aatcgtgctg attagctcta agttagtgta atcattgagt aaatctgcat tctcatcaaa
    12781 gtagaacgca tagggtgcaa cggattttgg cggcataaag taacgtactt ttaagcccat
    12841 tttcgcaaaa taggcatcag ttaatgaata actatcttgc agatattcca cacctaaaat
    12901 tgggtgaacg ttagtggttc ttgtgtaggt acgggtggtt gccacgctta aacagatcac
    12961 cggtggtttt ttatattttg cggtatattc aggcgatgcc aataaatgct taaacagctt
    13021 gccgtgtaac tcgccatagc cttccggcac gctaaattgc ggtttgtctt tattgtgatc
    13081 taacaacaat acgctgaaat cgtaatcacg cacaaaagaa gagaagttat taccgataat
    13141 gccatcaatg gttttgccgg ttttgtagtc gataacatag gttttcagcc actcaatcgc
    13201 agggaaaaac tcgcctttgc cttccacatc aatatccagt gagacaatat ccaatttcac
    13261 cgaataacga tccgcattcg ggttatccca gttcgctaaa tcgttaaaac ggttattgat
    13321 catcgcaatc gcattttgca ggttctgttt gcggttttcg ccacgggcaa ggttggcaaa
    13381 gttggtggtt aaacgggtgc tttgctccgg ttggtaatct tcattaaatt caatagtatg
    13441 cacttggcat ttaaaatttg ttgtcatttt attg
//
LOCUS       DANLXQ010000059        13444 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.059, whole genome shotgun sequence.
ACCESSION   DANLXQ010000059 DANLXQ010000000
VERSION     DANLXQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.059"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..778
                     /locus_tag="PXD94_001315"
     CDS             <1..778
                     /locus_tag="PXD94_001315"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL6286183.1"
                     /translation="ETDHDVSVWAARYIVDRINAFQPTKEKPFVLGLPTGGTPEKTYK
                     ELIKLYQAGEVSFKNVVTFNMDEYVGLPKEHPESYHSFMFKHFFDHVDIPLENINILN
                     GMAEDVNAECERYEEKIRFYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLTEDT
                     RIANSRFFNNDVSQVPKFALTVGVGTLLDAEEVMILVTGHNKALALQACVEGAVNHLW
                     TISCLQLHRRGIVVCDEPATQELKVKTVKYFKELEKNVAR"
     gene            859..2001
                     /gene="nagA"
                     /locus_tag="PXD94_001316"
     CDS             859..2001
                     /gene="nagA"
                     /locus_tag="PXD94_001316"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL6286184.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            2083..2397
                     /locus_tag="PXD94_001317"
     CDS             2083..2397
                     /locus_tag="PXD94_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL6286185.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(2476..3666)
                     /locus_tag="PXD94_001318"
     CDS             complement(2476..3666)
                     /locus_tag="PXD94_001318"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL6286186.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFINRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
     gene            complement(4029..5333)
                     /locus_tag="PXD94_001319"
     CDS             complement(4029..5333)
                     /locus_tag="PXD94_001319"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL6286187.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
     gene            complement(5343..7169)
                     /gene="oadA"
                     /locus_tag="PXD94_001320"
     CDS             complement(5343..7169)
                     /gene="oadA"
                     /locus_tag="PXD94_001320"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL6286188.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            complement(7209..7463)
                     /locus_tag="PXD94_001321"
     CDS             complement(7209..7463)
                     /locus_tag="PXD94_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL6286189.1"
                     /translation="MAPAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFP
                     EPVSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
     gene            complement(7653..8840)
                     /locus_tag="PXD94_001322"
     CDS             complement(7653..8840)
                     /locus_tag="PXD94_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL6286190.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFHEAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANLPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSFITSFSPFIEKHKNCFIVAFIVVSTAVLATIGRPAQVLVFVG
                     TLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIVVILMAVLSAMTLSKYISG
                     LMA"
     gene            complement(9115..9852)
                     /gene="pxpA"
                     /locus_tag="PXD94_001323"
     CDS             complement(9115..9852)
                     /gene="pxpA"
                     /locus_tag="PXD94_001323"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL6286191.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDGEHAVVFAERIKKELQAKGILVKA"
     gene            complement(9839..10768)
                     /locus_tag="PXD94_001324"
     CDS             complement(9839..10768)
                     /locus_tag="PXD94_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL6286192.1"
                     /translation="MIYIEIIQGFAHIQDLGRYGYRHLGVSHGGAMDSLALQAGNLLL
                     KNANNAPAIELTLGGMRITFDCDTPFCLTGALVEAELDGTPVFSYYRYTANARQTLTI
                     KRIVLGNYAYLCVAGGFLVPQVLGSASTALKAQFGGFQGRMLKAGDNIATGRRDSRLS
                     LMSIEPIDFTSRIRATASSEYEAFTADSRERFWQQGWQLQNNSNRMGYRFAEKALELT
                     ASLEMLSYAAPSGTVQVPPDGQPIVLMADAQTTGGYPKIACIIQADLGRLAQNPFGST
                     VQFEQVSREQAVEIYQKDQNYLETIRRKANESR"
     gene            complement(10765..11412)
                     /gene="pxpB"
                     /locus_tag="PXD94_001325"
     CDS             complement(10765..11412)
                     /gene="pxpB"
                     /locus_tag="PXD94_001325"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL6286193.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGRQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            complement(11506..12330)
                     /locus_tag="PXD94_001326"
     CDS             complement(11506..12330)
                     /locus_tag="PXD94_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL6286194.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVEQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            complement(12464..13288)
                     /locus_tag="PXD94_001327"
     CDS             complement(12464..13288)
                     /locus_tag="PXD94_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286195.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEEADFAKVKTVEAVQPKAIFPFY
                     VPAENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHF
                     AAYNDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNG
                     ELNTYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISI
                     GATRYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
BASE COUNT         3669 a         2989 c         3022 g         3764 t
ORIGIN      
        1 agaaactgat cacgatgtga gtgtttgggc tgctcgttat attgttgatc gtatcaatgc
       61 attccaacca accaaagaaa aaccttttgt gcttggctta ccaaccggcg gtacgcctga
      121 aaaaacctat aaagaattaa ttaaattata tcaagctggt gaagtgagct ttaaaaatgt
      181 ggtgactttc aatatggacg aatacgtcgg cttacctaag gaacaccctg aaagctacca
      241 cagttttatg ttcaagcact tttttgacca cgttgatatt ccgcttgaga atatcaatat
      301 cctcaacggt atggcggaag acgtgaatgc agaatgcgaa cgttatgaag aaaaaatccg
      361 tttttatggt aaaatccatc tgtttatggg tggggtcggt gtggatgggc atattgcgtt
      421 taatgagcct gcatcttcgc tttcttcccg cactcgaatt aaaacgctta ccgaagatac
      481 tcgcattgca aactcacgtt tctttaacaa tgacgtaagc caagtgccga aatttgcttt
      541 aaccgttggg gttggtacgt tgcttgatgc cgaagaagtg atgatcttag tgaccggcca
      601 taacaaagcc ttagcattac aggcgtgtgt ggaaggggcg gtaaatcatc tttggaccat
      661 cagttgctta caattacatc gccgtggtat agtggtgtgt gatgagccgg caacccaaga
      721 gctgaaagtt aaaacagtta agtatttcaa agagttagag aaaaacgtag ctcgctaatt
      781 ctgattttac aagcggttaa ttttgtaata ttttttacaa aaacgaccgc ctgttagcat
      841 ttaaagagaa gagagaaaat gaaccattat gccttaacaa actgtgtgat ttacaccggt
      901 gaacaagtgc tttaccaaca cgcattagtg gtgaaagaag ataaaatcga ctccattgtt
      961 ttggaagaaa atttaccgct ggatctgcaa aaaatcgacc taaaaggagc gaatctttca
     1021 gccggtttta ttgacttgca actgaacggc tgcggtgggg tgatgtttaa tgaagaaatc
     1081 actacccgca ctttagaaat tatgcaggaa accaatctgc gttcaggcac aaccagctat
     1141 ttaccgactt ttattacctc gcctgatgag ggagtgaaac aagcggtggc ggtaatgcgt
     1201 gagtatttgt cgaaacacaa aaaccaagca ctcggcttgc atttggaagg accttatcta
     1261 agtgtggcaa agaaaggagt acaccgtccg gaatttatcc gtgaaatcag ccctgaaatg
     1321 ttagatttct tatgcgataa cggcgatgta attacgaaaa tcacgattgc agcagaaaac
     1381 ccgaccgcag accatatcaa acgctttaat gatgcaggaa ttattgtatc tatcgggcat
     1441 tccaatgcca cttttgctca aaccaaacag cgaattgccg aaggggcagc gtttgctacg
     1501 catttacaca atgcaatgtc gccggtcagc aacgggcgtg aaggcggtgt agtaggggcg
     1561 gtgttggata gcgatattta caccggcatt atttgcgatg gcttgcacgt tgaatggggc
     1621 aacattcgca ttgccaaaaa agccaaaggc gataaattag tgattgtcag cgatgctgtt
     1681 gctgccgcag gaagtgatat tgagtcgttt atttttgtcg gtaaaaaagt cacggtgaaa
     1741 gatggcaaat gctacgatga attcggagca ttgggcggtg cggcaatcac gatgatcgaa
     1801 tccattcgca acgtagtgca acattgcgat attagcctga atgaagcaat ccgaatgagt
     1861 accctttacc cggcaaaagc gattggggta gaggaaacac tcggctcact tgaagccggc
     1921 aaaattgcca accttgcgat tttcgataat caatttgaag taaaagcgac tgctgtaaac
     1981 gggcaattac agtggcatta attttgctac aaattctaag cataacagca tataatccaa
     2041 aggcaggcat tgtgtctgcc ttttatttat agggagaata acatgcaatt taaagattac
     2101 ttagccactt ttccaactat tgatcatcta gccggcttaa acgtgataaa tagcggtgaa
     2161 gtgattcaac atattcctgc gattgaaggt aaattaggtt cactaaaact ttacaatgca
     2221 ctgtttgcga aatttgatgg gaaattaacc gctagtgcag caaaacaagg gttggaatgg
     2281 tttgccgaac acgttgaaga tgcgaaaaat aatgtgggta agcacccgaa tattgatcta
     2341 ttatttaggg taattgagca agatttatct ttaagtttag agccgattgt taaataagat
     2401 tgcactagtg aaaaataaca agcggtcatt atctgcaaaa tttttgcaaa taatgactgc
     2461 ttgtgtttaa gaggcttatt tcacggtaat gcgtgcaaat ttgcgtttgc cgacttggta
     2521 aacaaaagtg cctttttgag cagtttgttt cacatcttcc actttttcac catcaatttt
     2581 aaccccgcct tgttgggctg agcgaattgc ctctgaagtg gacggtacta agccggcttc
     2641 tttcagtaga gccgctaggc cgatttcacc ctcaaaggta aattcaggca cttcatcagg
     2701 aattgcccct ttttggaaac ggttgataaa ctcttgctct gctgcatctg ctgccgcttc
     2761 atcgtggaaa cgagcgataa tttcttttgc cagtagaatt ttcacatcac gcgggttttt
     2821 gccattttca acatccgctt ttaattgtgc aatttcgtct aatggacgga atgagagcag
     2881 gttgtaccaa tcccacatta aatcgtctga aatcgacata attttgccga acatttcgct
     2941 tggagcttca gttacaccaa tgtagttgcc gagagatttc gacatttttt tctcgccgtc
     3001 taagccgaca agtagtggga gtgtcattgc cacttgtggt ttttgaccgg ctgatttttg
     3061 taactcacgt ccaactaaca agttaaaggt ttggtctgta ccacctaatt ccacgtcagc
     3121 tttcagttct accgagtcgt gaccttgtag tagcggataa ataaattcgt ggattgcgat
     3181 cggttgttgg ttcgtgaaac ggtttttgaa atcttcacgt tctaacattc gagcaacggt
     3241 gtagttggag gctaagcgga tcataccttc cgtacctaac ttgcctaacc attcggagtt
     3301 gaatacgatc ttggtttttt gtggatctaa aattttgaag atctgctctt tataggtttc
     3361 ggcattgcgt aacacgtctt cacggctaag tggcggacgg gtagagtttt ttcctgatgg
     3421 atcgcctacc ataccggtga aatcaccgat taagaaatag acttcgtgtc cgaattgttg
     3481 gaattgacga agtttgttca gcacaacagt atgacctaag tgaatatccg gtgcagttgg
     3541 gtctgcccct aatttaatac gcaatgggcg gttttctttt aatttggtga ttaaatcttc
     3601 ttctgaataa atattttcca cgccacgttt aagttcggca aggactgatt cgatagcttg
     3661 agtcatttat cttcctttta gtcttgaata taaaaaatgg atctattata gcggtatgaa
     3721 ttgaaattga taggctatag gcgaaaaaaa acgccgtatt ttgtggggga atacggcgta
     3781 aagagttgga gtgattacct attatttttg aagtgattta atgataatga ttatcatctg
     3841 ttaagtcaat actttttttg aaaattttca ataagaatta ttctcatcaa ttaagtaaga
     3901 tggatactac ggcaattagt gaagaatgta cagcggaatt ttcgtattgt aagagtgttg
     3961 gtaagcggta tttttagaaa aaacctccga gagcggttat ttttcggagg ttttttgcaa
     4021 atgaggattt acatcgttga aatgtatttc aacatcaccc ccgctgcaat cgcagagcca
     4081 atcacgccgg caacgtttgg gcccattgcg tgcattaaca agaagttttg tttatcgtac
     4141 tcttgtccga ctttgtttga tacccgtgca gccatcggca cggcagaaac acccgctgag
     4201 ccgataagtg ggttgatttt gtgctgacta aagcggttca ttattttcgc cataatcaca
     4261 ccgcttgcag tgccgatccc aaaggcaatc acgcccaata ctaaaatgcc tagtgtttgc
     4321 ggttgtaaga atttgtctgc gaccaatttt gcccccaccg ataaccctaa gaaaatcgtc
     4381 acgatattga ttaacgcatt ttgggcggtg tcgtttaagc gttctactac gccactggtt
     4441 cgcagtaagt tgccaaagca gaacattccc aataacggtg cagcatccgg cagtaagagt
     4501 gccactaata atagcaacac gatagggaaa ataattttct cacggttgct tacattacgg
     4561 agctgaacca ttcggatttt acgttcttgc tcggtggtta aggctttcat aatcggcggc
     4621 tgaatcagtg gcactaacgc catataagaa taagccgcta ccgcaatcgc acctaataat
     4681 tcaggggcaa gtttgctggc aagataaatc gcggtcgggc catctgcccc gccgataatc
     4741 ccaatcgcag cggcttgtgg caatgtgaag tcgataatgc ctaaccagtt tagccctaat
     4801 gcacccagta cggtagcaaa aatcccaaat tgagccgcag ccccaagcat taacattcga
     4861 gggttggcaa gtaaagggcc gaaatcagtc atcgccccga cacccataaa gataataagc
     4921 ggagcaacgc cgtagccgat agcaacttta tagaataatg ccaacacacc ggcggtatag
     4981 cccatatcgc cagccattac ttcaagctca ttttgaacag agggagcagc cagtgcgatg
     5041 gcatctttaa tcgaatgcag atcaaggctt gtactattta ccttagcagc aataattgcc
     5101 agttgctcgg cagaaccgct gtggagtaaa ttgtctaatg ccgtcattgc catacctgct
     5161 tccggaatgt ttgacatcaa accaccaaag ccaataggca gtagtaaaag cggttcgaat
     5221 tttcgggcga tggcaagcca aagcaacact aaactgatga caatcataat cgcttgcccc
     5281 agttctaaat gcattagccc cataccttta aaaagtgcaa taatactttc cataccggac
     5341 tcctaggcaa tactcattaa agtatcattt accgccacaa catcgccttg tttgacatta
     5401 atgccttgaa tcgtgccgga ttttgccgca caaatttggg tttccatttt cattgcttca
     5461 agaatcagta acacatcgcc ttcagcaact tgttgcccct ctttcacttc cactttcaaa
     5521 atgttccctg ccattggagc attcaccggt tcggcattgg ctgatagcgt atttgcagat
     5581 tttggtgcag aatcgaccgc ttgcggagcc ggtgttgctg gtgttggagc gatattggtt
     5641 atatcgccac cttcactcac cttcaccaca aaggctttgc cttcaagctc aacggtataa
     5701 atggctgaac cggattgtgt ttgggctttt ggtgcagaag cagcggaggc ttgtgccggt
     5761 tttgcttcat cagcacttgg cactggctca aaggcagacg gattattgcg gttttccaaa
     5821 tatttccagc ccacttgttg gaagagtgcc acaatcaata catcatcaat catgttttca
     5881 gatagcgtaa tgcctttttc ttttgcactt gcagtcactt ctgccactaa tttttccagt
     5941 tccggcggaa gatgatcggc tgggcggtcg gtaatcggct ctgctccctc aagcactctg
     6001 tcttgcaagg ctttgtttac cggtgccggt gtgcgaccat attcgccttt taaaatacct
     6061 gcagtttctt tggcgatagt tttataacgt tcgcccatta atacgttcat caccgcttgt
     6121 gtgccgacaa tttgtgaggt tggtgtcacc aacggaatat tgcctaaatc ttcacgcaca
     6181 cgaggaattt cttgtaaaac caagtctaat ttatccgacg cattttgttg cttgagttgg
     6241 ctttcgaggt tggtcagcat gccacccggc acttgggcaa ccaaaatacg gctatctata
     6301 ccacggagct gtccttcaaa ttttgcatat tttttacgca cgttacggaa gtaggcagag
     6361 attttctcca gagctggaat gctcaagccg gtttcatacg gcgtgccttg aagtgtggcg
     6421 acaatagact cggttgccgg gtgaccgtaa gtgccggaca ttgatgaaat ggaggtatcg
     6481 acaccatcga caccggcttc aatcgctttg agcaacgcca tttcagccat accggtggtt
     6541 gaatggcaat ggagatgtag ctcaacgtcg tagcgttttt taatttcgct gactaattca
     6601 taggctgcca ttggggttaa aatgcccgac atatctttga tcaccaacga atccacgcca
     6661 atttccaata attgctcggt tacatccaac cacgtttgta gggtatgaac agggctggtg
     6721 gtgtaactca aagtaccttg tgcgtgcccg ccgtgttttt tgaccgcttg taatgcggct
     6781 tgcatattgc gagggtcgtt gagggcatca aatacacgga acacactcat accgtttgcc
     6841 accgaacgct cgacaaattt atctaccaca tcgtcggcat aatgacgata acccaataag
     6901 ttttgcccac gtagcagcat ttgcattggg gttttcggca ttgctttttt cagctcacgt
     6961 aaacgcaccc acggatcttc gcctaaaaaa cgaatacaac tgtcaaaagt tgccccgccc
     7021 caagtttcta acgaccaata gccgatatta tccagctcgg aggcaatcgg taacatatcg
     7081 tctaagcgta aacgagtcgc aaaaagggat tggtgtgcat cacgcaaaac cacatcggta
     7141 atcgcaattt ttttcggttg agttgtcata atcaaattcc ttctaattat tttatttttg
     7201 aaatcttgtt aatgttgccc gttaattcga cgatgatgag caatcgcagc ggcaatcaca
     7261 gggcgtaaac gctctaaatc atctggggga gattgaggca cagttttaga ggctggaata
     7321 gaaacaggtt cggggaaata ttgattgata atcttggaaa taaagctgat ggcataaatt
     7381 aaaaccagca ggaacagcat aacaaatccc attccggcta ccattaaatt tataccttcc
     7441 gcaaagagtt ctgcaggtgc cataatttat ccttaaaatg tagaaatgat gaagaaaatg
     7501 tagaaaaaat tcagatttat ttcaaattat ttagcctcaa aatgagtggt agatcacaaa
     7561 ctttaaacgc aagcggtcag attttacaaa ttttttacaa gagtagttaa aatttgcaaa
     7621 acctgacctt aggttagctt gaagctaagt cgttatgcca ttagccccga aatatatttt
     7681 gaaagggtca ttgcacttag caccgccatt agaatcacca caatcgcccc tgaaacggtc
     7741 atccacacag ggtgtttgta gtcgcccaca atcttggttt tgtgagctgc cagcagaatt
     7801 aacccgaggg aaatcggtag aattaagccg ttaagcgtcc ctacaaacac caagacttgt
     7861 gccggtctgc cgatggttgc cagcacggcg gttgaaacca caataaaggc gacaatgaaa
     7921 cagtttttgt gtttttcgat aaatggcgag aatgaagtaa tgaaggaaac cgaggtataa
     7981 gccgccccaa tcacagaggt aatggaggct gcccaaatca ccacgccgaa gatcatcaaa
     8041 ccgacatcgc cggcaacgtg ggcaaatggg gtagccgcag ggtttttcgg atcaagttgc
     8101 acgcccattg acaccacgcc cagcaccgct aaaaacagta ccacacgcat aattgaggcg
     8161 attaaaatgg ctgacaccga gcttttgctc acctctttta aattctcttt gcctttaatg
     8221 cctgcatcaa gcaaacggtg cgcaccagca aaggtgatgt atccaccaac agttccgccc
     8281 actagcgtga caatcgcaat cacgtctaat ttttccggca caaaggtatg caccaccgca
     8341 tcgtcgattg gcagattggc ttttacggca acgaagatgg ttaaagcaat catcacaaag
     8401 cccataattt gtgcaaatct gtccatcact ttaccagctt catggaacag gaacacgccc
     8461 acggcaatcg caccgctgac caccgcaccc atttcagggg aaagtccggt gagaatgttt
     8521 agccctaaac ctgcaccgcc cacgttgccg atattaaagg ctaaaccgcc catcacgata
     8581 agcagagcca gaaagtaacc tgcccccggc aatacggcat tggcgatgtc ttgagctttc
     8641 ttttccgata ctgctacaat tcgccaaata ttcagttgag caccgatatc aagcaagatc
     8701 gagagtaaga tcacaaagcc gaagctggca agtagggttt gggtgaaggt ggctgtttgg
     8761 gttaagaacc ccggcccgat ggcggaggtc gccattaaaa atgctgcccc gagtagggcg
     8821 ctacggttgt tgtttgacat tgtgttggtt ccttttttgt tgttaatgag tcattattcg
     8881 tggatttatc atggtaatat tcccctcttt agcaaagagg ggttagggga gatttggcag
     8941 tagaactcat cgaagtgaga gagttaataa tgctcaattt aacctcttga aattacttat
     9001 tttctccgta aatcgcttct gtcaaatctc ccccagcccc tcttttctaa agaggggagt
     9061 tcgatctcca agagttttat ttctggtttt ccgtccattt tgactaaaac aggatcaggc
     9121 tttcaccaaa atcccctttg cctgtaactc ttttttaatt cgctcggcaa ataccacggc
     9181 gtgttcgccg tcaccgtgta agcaaatgct gtcggcttta atcgccacat taccaccatc
     9241 aacggttggt acagtgccgt ctaacaccat ttgcaagact tggctgatcg cttcatcatc
     9301 gctttctacc attgcatcat cacgggtgcg aggcacgagc gagccgtcag ctaaatagtg
     9361 gcggtcggca aagacttcgg aaatggtttc caaaccgtgc tgctcggcaa tgctcaacat
     9421 caggctgccg gataagccca tcagtttgag gttgggatcg aactgtttga ccgtattggc
     9481 gataagggtg gcaaggtgag ggtctttggc ggccatattg tataaagcgc cgtgcggttt
     9541 aacgtagctc atcggcatat tttccgcatc acataaggct ttggcggctc ccagttggta
     9601 gagcagacag gctttaagtt catcatcggg cagttgcata ttggtacgcc caaagttttc
     9661 cctatctgga aaactcgggt gagcgccaac tcgcacgctg ttttccttcg cccagttaat
     9721 cgctttacgc atttcattgt aatcaccggc gtgcaagcca cagcagacat tcgccgaagt
     9781 aacgagctgt aataatgaat gatcgttgcc acaaccttcg gcaagatcga catttaaatc
     9841 aacgtgattc attcgctttt ctccgaattg tttccaaata attttgatct ttttgataaa
     9901 tttcgaccgc ttgttcacgg cttacttgct caaattgcac cgttgagccg aacggatttt
     9961 gtgccaaccg cccgagatcg gcctgaataa tgcaggcaat tttcggataa ccaccggtgg
    10021 tttgggcatc tgccatcagc acaatcggct ggccgtccgg cggcacttgc accgtaccac
    10081 tcggggctgc gtaggagagc atttcaaggg aagcggtcaa ttccaacgct ttttctgcaa
    10141 aacggtagcc catacggttg ctgttgtttt gcagttgcca cccttgttgc caaaagcgtt
    10201 cacggctatc tgcggtaaag gcttcatatt cgctcgaagc ggttgcccga atgcgtgagg
    10261 tgaaatcaat cggctcaatc gacatcaaag aaagtcggct gtctctgcgg cctgtggcga
    10321 tgttatcgcc ggcttttaac attctgcctt gaaaaccgcc aaattgagct ttgagagcgg
    10381 tacttgccga acctaacacc tgcggcacca gaaaaccgcc tgccacgcat aaataggcgt
    10441 aattgccgag aacaatccgt tttatcgtca gcgtttgacg ggcgttggca gtgtagcggt
    10501 aataggaaaa aaccggcgtg ccgtccagct ccgcctccac caatgcaccg gtgaggcaaa
    10561 atggcgtgtc gcaatcaaag gtaatccgca ttccgccaag ggtaagttca atcgcaggtg
    10621 cattatttgc attttttaac aataaattac ccgcttgtaa ggcaaggctg tccatcgcac
    10681 cgccgtggct cacgcctaaa tggcgataac cataacgccc taaatcttga atgtgggcaa
    10741 aaccctgtat tatttcgata taaatcatag cgttatactc tccgccacaa atctaacatt
    10801 atcgccggct gtaagtagcg ttggcggatt gtgttgaata tcaaacaacg gtaactcggt
    10861 tcgtcctaac agttgccagc cacctggtga ggtaaaggga taaatgcctg tttgcctgcc
    10921 gccgatgccg accgaaccgg cagggacttt ggtgcgaggc acgtttcggc gtggggtgtg
    10981 taaattttgc ggtaagccgt tgaggtaagg aaagccgggt tgaaagccga tcataaacac
    11041 ggtgtaagtc ggttcggtat gacggcggat aatctcttcg ggagtggtat tgtggaattg
    11101 ggcgacttca aacagatctt cgccaaattc accgccgtaa tggacaggga tttcaatcag
    11161 gcgtggggat tgtgcggctt gggcttggtc gttttcccag agttgttgaa ccgcttggat
    11221 aaggcgtgtg ctatcggtgc caatggcgtg aaatagggta aaattgttca ttcctaccac
    11281 cacttccaac acgccatcta aggctcttgc ctgttctgcc acccgccaaa tctgttgctg
    11341 tttggcaagc tctgcaggcg gttcggctaa gcagagcagg gaatgttcgc tggtatgatg
    11401 aatcgtcagc ataattgccc tcaaattgtt acaaaaaggt aacaactttt tacgctgata
    11461 tatccagact gtcaatttta ttttgttgaa aatgtgaaat agctcttaat tttcagaaat
    11521 ttgatcattt cttattcgcc aaaacgttgc aaatctgggc ttgcctgatt ctgtgacacc
    11581 tcgatattta taggtaatga ttgagccaat cggtggcgga ttttctcgtt ctttatcttt
    11641 aaaaccgctg ccaatccgaa aacgtccacg ttgattttca caagtgattg ccccgagttt
    11701 atcggtatat ttccctttgc ctttatgatg ggcaaccaca gtgcattcct catctaaaac
    11761 cggtttgatt ttcaaaattt gggcagaacg accttgaata tagcctgcat tcgggttgcg
    11821 aacgataacc ccttcgccac cttgttccag cacagattgg taaaattgtt gaaggtgggc
    11881 tttatcttta atcggaattt gctcaataat agcgatatgg agcgttggat gttgcttcag
    11941 ataattctct aaggtcgcca gccgttcaat caaattccct tgtgcatttg gcacatcgaa
    12001 cacctgtaat ttcagcttgt gccaaccttt gggctgagcg gaacgcacaa tgcttgaaat
    12061 ttcctcaaat ttgccccgtt cgctaaacag ttcgccgtca atcgcaaaag gagggaaatc
    12121 ttgaataaaa tagtcgggtg gattgagcgg ataaccttgg cggctgatga gctgttgacc
    12181 gtcccaaaag cctcgcacgc catcgagttt ttctgacatt acccagccac tgatgtcttg
    12241 atcttgatat tgctcaacga gcattaactc gggcgatttg gcaaaaatag ggttaccaat
    12301 gaagagtagg aataagagca gttttttcat agtctctccg aaatagtgcc tccgaatatg
    12361 caaggcacta taaaggaaag cgtgcaaaca gtcagaaatg aatctcaatt tttcgacgca
    12421 gatcgcaaaa ttttccgaaa aactgatcgc ttgagtgaga aatttaacgt actgtttgaa
    12481 ttaaagcaga aatatcctcg ctaaaggttt ccaacttcgc catttcttca atttggctat
    12541 gagaatattt cttcacattg taaacgacca ccatacttac atcacctact tgcaataaat
    12601 tactttcgcc aaaaggcgtg tagcgtgttg ccccaatgct gataatcgct ttagttgggt
    12661 agtttgcctg ttttaacatt tcggaaatgt tgttcatcgg accttcatcg ggctgattat
    12721 tcattctctc tacaatccaa tcgagcaatt tttgatggaa atagctgtac tccaccgcag
    12781 ggctgtcgat gccgtaggta ttcagctcgc cattacgttt atggaaacaa gcgatgttgt
    12841 aattatccag cgtgccgcct ttgttgaagt tatctaacgg aagatgaatt gccgaaagcc
    12901 ctttagacga agccccccag tttttctttt cacagatttt attcgcattc ggacggcgga
    12961 tagagcagtc attgtaagcc gcaaaatggg ttggcgtgag cgaaaccact tgctgatctt
    13021 ggtagccaat ttcacaaatt aacgccactt caggctcaat ttgcagatta tcagcatcac
    13081 tcgggaaatt aatcgcagta tttgaaagcg gataggtgga taaaaactga taatccccca
    13141 aattttccgc cggcacataa aacgggaaaa tcgcctttgg ctgcactgct tccactgttt
    13201 tcactttcgc aaaatccgcc tcctctccgg cttgttctaa atgtccagca aaattgccgg
    13261 ccacgcctaa accgataaag tgttccataa tctactcctt aattggtaaa agttggataa
    13321 aattttaccg cttgtaacca aaaattgcga atgtttattt tgatctgatt gatagaaaat
    13381 tattgaattg agattaaatt tagccatatt ttgaacataa aattagcgct ttgttataaa
    13441 aaca
//
LOCUS       DANLXQ010000060        13344 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.060, whole genome shotgun sequence.
ACCESSION   DANLXQ010000060 DANLXQ010000000
VERSION     DANLXQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.060"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            342..986
                     /gene="rppH"
                     /locus_tag="PXD94_001328"
     CDS             342..986
                     /gene="rppH"
                     /locus_tag="PXD94_001328"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL6286196.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            988..1776
                     /locus_tag="PXD94_001329"
     CDS             988..1776
                     /locus_tag="PXD94_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL6286197.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            1784..2581
                     /gene="lgt"
                     /locus_tag="PXD94_001330"
     CDS             1784..2581
                     /gene="lgt"
                     /locus_tag="PXD94_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL6286198.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGGILIMAWAYKVNKVK"
     gene            2668..3336
                     /locus_tag="PXD94_001331"
     CDS             2668..3336
                     /locus_tag="PXD94_001331"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL6286199.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            3406..3666
                     /locus_tag="PXD94_001332"
     CDS             3406..3666
                     /locus_tag="PXD94_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL6286200.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            3663..3995
                     /locus_tag="PXD94_001333"
     CDS             3663..3995
                     /locus_tag="PXD94_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL6286201.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            4122..5153
                     /gene="trpS"
                     /locus_tag="PXD94_001334"
     CDS             4122..5153
                     /gene="trpS"
                     /locus_tag="PXD94_001334"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL6286202.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(5217..5972)
                     /locus_tag="PXD94_001335"
     CDS             complement(5217..5972)
                     /locus_tag="PXD94_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL6286203.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(6028..6555)
                     /gene="ppa"
                     /locus_tag="PXD94_001336"
     CDS             complement(6028..6555)
                     /gene="ppa"
                     /locus_tag="PXD94_001336"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL6286204.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
     gene            6772..6847
                     /locus_tag="PXD94_001337"
     tRNA            6772..6847
                     /locus_tag="PXD94_001337"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6805..6807,aa:Lys,seq:ttt)
     gene            6870..6945
                     /locus_tag="PXD94_001338"
     tRNA            6870..6945
                     /locus_tag="PXD94_001338"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6903..6905,aa:Lys,seq:ttt)
     gene            6967..7042
                     /locus_tag="PXD94_001339"
     tRNA            6967..7042
                     /locus_tag="PXD94_001339"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7000..7002,aa:Lys,seq:ttt)
     gene            7135..8169
                     /gene="mltG"
                     /locus_tag="PXD94_001340"
     CDS             7135..8169
                     /gene="mltG"
                     /locus_tag="PXD94_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL6286205.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            8180..8800
                     /locus_tag="PXD94_001341"
     CDS             8180..8800
                     /locus_tag="PXD94_001341"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL6286206.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            8800..9777
                     /locus_tag="PXD94_001342"
     CDS             8800..9777
                     /locus_tag="PXD94_001342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL6286207.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            9908..10150
                     /locus_tag="PXD94_001343"
     CDS             9908..10150
                     /locus_tag="PXD94_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL6286208.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            10143..10427
                     /locus_tag="PXD94_001344"
     CDS             10143..10427
                     /locus_tag="PXD94_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6286209.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEYIRGGLRMHTHQSHTIYYRIKADHIFIVRILHNQMNHKRYI"
     gene            10432..11220
                     /locus_tag="PXD94_001345"
     CDS             10432..11220
                     /locus_tag="PXD94_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL6286210.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
     gene            11230..11751
                     /locus_tag="PXD94_001346"
     CDS             11230..11751
                     /locus_tag="PXD94_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL6286211.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            11826..12527
                     /gene="lpxH"
                     /locus_tag="PXD94_001347"
     CDS             11826..12527
                     /gene="lpxH"
                     /locus_tag="PXD94_001347"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL6286212.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            12524..13314
                     /locus_tag="PXD94_001348"
                     /pseudo
     CDS             12524..13314
                     /locus_tag="PXD94_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         3963 a         2841 c         2777 g         3763 t
ORIGIN      
        1 acatctttat ccacaaatgg tggggataac tctgctttat ttgcgaagca aaaattgccc
       61 tgcttattca ttttaaaatt taaacttatt ttgacgagaa aaaacgcaac aacttgatct
      121 ttatccagat cattgcttaa gaaaaagatc gcatttttag atctgattaa ttgctctgtt
      181 ttacaacgtt ccgcctgttt atcctcaact tataccactt atattcaata gttatccaca
      241 aagttattca caagcggtta tattttatgg tttttttgca aatcaaccca aagtatgaaa
      301 caattctccc attctagaat ttttatcaat taggtggtcg agtgatcgat ttcgatggct
      361 accgcccaaa tgttggaatc gttatctgta ataagcacgg gcaagttctg tgggcaaaac
      421 ggtttggtca aaactcttgg caatttccgc aaggtggaat taatgagggg gaaaacattg
      481 aagcagcaat gtaccgtgag ctttatgaag aggtcggctt aagtaaaaaa gatgtacgat
      541 tgctttgggc gtctaaatat tggctaaaat ataaactgcc aaaacgcttg gtacgtaatg
      601 aaggctctgg tcctgtctgt atcgggcaaa aacagcgttg gtttctactg caattaatcg
      661 gtgatgaaag cctgattaat ttgaaaacca cgaaaaatcc ggagtttgac ggctggcgtt
      721 gggtcagctt ttggtatcca gtccgccaag tggtctcttt taaacgagat gtttatcgca
      781 aagcgatgaa agagtttgca caggttttgc aaaacaatat tcaaaaagcg actgacgata
      841 aaaccggtga gaaaccacgc attgagcgtg gcgagacttc gccccatact tataaaaaaa
      901 cctctcagga ttctgccaaa gcctataaac gagaagctcg taagccttat cgtccttggc
      961 atcaacataa tcaaaaagga aaataagatg actattttat taagctgcct ttttttaggg
     1021 gtgtttgtcg gctttttagc cggcttgttc ggcattggtg gtgggttaat catcgtgccg
     1081 agtttggtct tcctactgcc catggcaggt gtaccgccgg aatatataat gtccaccgca
     1141 ctcggcacat cattttcaac cattattatt accgcctttg catcggcaca acgccatcat
     1201 aagttaggca atgtcgatat ccaaaccagt aaatatttta ttccagcgtt gatggtttcg
     1261 gtttttctat cgggcttggt ggtaagccgc ctcgatccgc aactgatgtc tagaattttt
     1321 gcggtaatgg tactttacct tgcaggtaaa atgatttttt caataaaaag agcaccgaaa
     1381 aataaaccac ttaccgttca aacgacgata atcggaggtt caatcatcgg tgcgttagcc
     1441 agtatggcag ggatcgcagg tggagccttt attgtaccat ttttaaacga tcgaggctta
     1501 gaaatgaaac gtgccatcgg cacttcctca ttttgcggag catttttagg tttatctggc
     1561 acaatcagct ttattgtaag tggctggggc ttagaaggaa tgcctgatta ttctctcggt
     1621 tatgtttatt taccggctct gtttggtatt acggcaactt catttttcac ctcaaaaatg
     1681 ggggcaagtg ccgccaacgc tctacctgtg ccgatgctga aaaaagcctt tgcggtgctt
     1741 ttggtctgta tcgccatcaa tatgttttta aaataaggac actatgaacg aacaatttat
     1801 ccaatttcca caaattagcc cgattgtgtt tgaaatcggt ccgattgccc tgcgttggta
     1861 tgggctaatg tatttagccg gctttgcctt tgcctactgg ctgggaatga gacgggcgaa
     1921 aaattccaat ggagtttgga caaccgagca agtggatagc ttagtctata cttgcttctg
     1981 gggcgtagta ttaggtgggc gtatcggcga tgtgttcttt tatagcttcg accgtttctt
     2041 acaagatccg ctctacctct ttcgcatttg ggaaggcgga atgtcctttc acggcggttt
     2101 aatcggcgtc attgtagcaa tgatttgggt ttcctaccgc caaaaacgca atttctggca
     2161 gacagcagat tttatcgcac cgctagtacc gttcgggcta ggtatggggc gtatcggcaa
     2221 tttcattaac gatgaacttt gggggcgtgt aaccgatgtg ccttgggcgg tgttatttcc
     2281 aagcggtggc tatcttcccc gccacccgtc acagctttat gaagcctttt tagagggtgt
     2341 ggtgttactc tttatcctca actggtttat tcaaaagcct cgcccggcag gtgcggtttc
     2401 agggttattt ttaatcggct acggcacatt ccgctttatt gtggaatact tccgagaaat
     2461 cgaccataac gtcaatacca ccgcagattt aatcactcgt gggcaactac tctctttacc
     2521 gatgattatc ggcggcatac tgattatggc gtgggcgtat aaagtaaaca aagtgaaata
     2581 aacgtagggg caagtcaata tttgccctca ttacaaaaat tgcaaaaaat ttgcagaaaa
     2641 cgaccgcttg tgtttgaagg atatactatg agtaaaaaat acaaagtcat cggtttcgac
     2701 ttagacggaa cgctaatcaa caccctcccc gatctcactc ttgtcgccaa ttcaatgttt
     2761 ctagaagccg gtttgccaac ggttacacaa gaaaaagtgc tgacttggat cggtaaaggg
     2821 gcagatattt tcttccaaaa tgcggttgct cacaccggca gaattttcca agccgatgag
     2881 ttggtgaaaa tgcgagtgag ctttgataaa tactacgcca cctatgtgtg cgaggaaagc
     2941 acccttttcc caaatgtgaa agaaacctta gaaatcctca aagcaaacga ctacccgctg
     3001 gtggtgatta ccaacaaacc gaccaaatta gtcgagccgg tgctttccgc tttcggcatt
     3061 tatcatttat tcgatgaaac ccttggtggg caatcgctcc ccaaaatcaa accgcaccct
     3121 gacccgatgt tttttatctg cgaaaaattc ggcattaccc catcagaatt actttttgtc
     3181 ggcgactcag aaaatgacgt gattgccgcc aaagccgccg gttgtgatgt ggttggacta
     3241 acttacggct ataactacaa tgtgccgatt gaacaatcta acccgacttt tgtgacatct
     3301 gaatttaaag atgtgttgaa aattgtgggg ttataaagaa aacatttgtt ttcttttgat
     3361 gtttgcttta gactttccga acctaaagat ttaaaggagt aaagtatgcc aacaatcgca
     3421 ttacgtcagg tcggtggctc ggttgcaatg gtgattccgc ctgctttttt agaggcactc
     3481 aagctctccg tcggtagcaa agtggatttg tctttaaacg acaaccaact cgtgattaat
     3541 cctgcccgcc caaaagtgaa atacagctta gaggaattat taagacaatc cgattttgag
     3601 gcagcaagga atgaagaaag ccaatcgtgg ttatccgagc cggcaatagg aaaagaaatt
     3661 ttatgaaacg tggcgatatt tactggctcg atcttgatcc aaccttagga cacgaacaat
     3721 caggcttccg ccccgttgta attgtttccg caactgcatt taatctagcc agtaaactgc
     3781 ctgtggttgt gccgattact aacggtggag cttttgctga aagactgggc tttgccgttt
     3841 cattaaaaga tgccggctta aaaacaacag ggattattcg atgcgatcaa cctcgcacag
     3901 tcgatattgc acaacgccat ggaaaattta tcgaagcctt acccgaaagc ctacttgatg
     3961 aaattatggc aagggtttca gtgatttttg agtaagatac aagcggtctg atttgctcta
     4021 ttttttgcaa aatcatcaat aaatctgacc gcttgtactt ttatcccttc ccaataaccg
     4081 ttaagataac ctatattttt caaatcaaac gagaagataa aatgagcaaa cctattgtat
     4141 ttagtggcgt gcagccctca ggtgaattga ctatcggtaa ctacttaggg gcgttacgca
     4201 actgggtgaa aatgcaagat gattatgact gtgtatattg cattgtcgat caacacgcca
     4261 ttaccgttcg tcaagatccg gagtcgctac gcaaatcaac tttagatgtg ttagccctgt
     4321 atttagcttg tggcattgat ccaaacaaaa gcactatttt tattcaatcc cacgtgccgg
     4381 aacacgccca attagcttgg gtgctgaact gttacaccta tttcggtgaa atggggcgta
     4441 tgacgcaatt taaagataaa tcagcacgct atgaagagaa tgtcaatgtg ggcttattta
     4501 cttatccggt attaatggcg gcagatattc tgctttacca agccaaccaa gtgccggtag
     4561 gcgatgacca aaaacaacat ttggaaatca cccgtgacat tgccaaccgt ttcaatgcct
     4621 tatacggcaa aaaagattcc gaaggcaacc tgatcgagcc tgtttttgca gtgccggaag
     4681 tctttattgc gaaatccggg gcaagagtga tgtcgctact tgagccgacc aaaaaaatgt
     4741 caaaatcgga cgataatcgt aacaatgtga ttggactatt ggaagatccg aaagcggtag
     4801 cgaagaaaat caaacgggca atgaccgatg gcgatgaacc gccagtagtg cgttatgacg
     4861 tacagaataa agccggtgtc tcgaatttat tggatattct ctccgccatt accggcaaaa
     4921 ccattgccga attagaaacc gaatttgaag gcaaaatgta tggtcatttg aaaacagaag
     4981 tagcagataa ggtttccgaa ctattaacca atctgcaaga acgttatcac cactaccgcc
     5041 aagatgaagc attattggaa aaaatctacc gtgaaggggc agaaaaagct cgagctcaag
     5101 ccaaagcaac gttagataag gtatatgaat tagtcggttt tgtgcgttac taacacttaa
     5161 tgaaaaatgc tcttgaggat tcaagagcat ttttgcaaaa aagaaccgat tgtcaattat
     5221 ttacgggcgt tataaatctt cattgcttcc ggcacaaggc gggctaagtt ttcttgacgg
     5281 tcagcatttg atgggtgagt tgagaaaatg gacaagcctg aatcgccatt ggctttactc
     5341 atttttaccc aaacattcgg cgctgctgat gggttgtagc ctgattctgc cattaaaatt
     5401 aacccaactt cgtccgcctc agtctcttgg ctgcgagaga atggcttggt ggcaatcaaa
     5461 tccacgccag tgctgagtaa accttcggtg cttacgcctg ttgaggcagt cacagccaca
     5521 tctgcaatag cacctacaat actggtaata gcgctaaccg tacggtctga tttaccgtgt
     5581 tctttcagtg cgtgagccat ctcgtgtccc attaccactg caatttcatc gttcgataaa
     5641 ttaagtcttt ccactaaccc cgtgtaaaac gccattttac cgcccggcat cgcccacgca
     5701 ttgagttcat ccgatttcaa tacggtaatt tcccaatgaa aaggtacacc agtctggttg
     5761 gctctttccg cataaggctt cattttatga aaaacagtgt gaatccgctt agcggtggca
     5821 gacgaattgt caatcgcatt ttgctgacga gcttgggttt tcacttgagc ataactttga
     5881 gccgcttctt ggttaatgct ttctgttgtg gcacaagcgg taatcactgt tgctaaaaaa
     5941 actgaataac ttaatttttt gatgaatttc attttatatc ccacaaaaaa tgccaaacaa
     6001 atttgcttgg cattcaaaaa taaataatta tttcttagca cgctcgaatg attctaagat
     6061 ttcttggcga gcttcgttta catcgcccca accttctact ttcacccatt tgcccgcttc
     6121 taatgcttta tagctttcga agaagtgctg gatttgtgct tttaataacg ccggtaagtc
     6181 acctacatct ttgatgtggt catactcttt acttaatttg gtgtgtggaa cagcaaccac
     6241 ttttgcatca ccgccggctt catcggtcat ttttaacaca ccgacaggac ggcaacgaat
     6301 caccgaaccc ggttgtaacg gatatggtgt tggcactaat acatcaaccg ggtcaccgtc
     6361 tgatgataag gtgttattca cataaccata gtttgccgga tagaacattg cggttgccat
     6421 aaagcgatcc acaaataatg ccccggtttc tttgtccact tcatatttga ttggatcaga
     6481 gtttgccgga atttcaatta caacataaat atcgtccggt aattctttac cggcaggaac
     6541 taattctaag cccattttgg tttccttcta taataggtga aataaacggc gtgattatag
     6601 cctattttca gaaaaagata aaagcccgtt tctcttttca ttttccgcca acaaatccgg
     6661 ctaatttttt gctaaattgc ttacaaaccg cacactcaac catttttcga taaaaaaaac
     6721 ttgccaaagt tttaaaaatc cgtaagatat gccctcgtta cgcaagatta tgggtcatta
     6781 gctcagccgg tagagcagcg gacttttaat ccgttggtcg aaggttcgaa tccttcatga
     6841 cccaccatta atcttcaaaa cacctctaag ggtcattagc tcagtcggta gagcagcgga
     6901 cttttaatcc gttggtcgaa ggttcgaatc cttcatgacc caccattaat ttcaaaactc
     6961 ctctgagggt cattagctca gtcggtagag cagcggactt ttaatccgtt ggtcgaaggt
     7021 tcgaatcctt catgacccac catttactca aatcccattt gcaaattttt ctgaaaaaac
     7081 aaccgcttta tagtagaatt cagtcaattt ttatcacttt tattttattc atcaatgcta
     7141 aaaaaaatcc ttattgcatt aggcttagcc ggcgttattg cttgcggtgg actgttttac
     7201 ggctaccaaa aactcacctc tttagccgaa cacccgatca cggttcagcc gaatcaactt
     7261 ttcgtattgg aaaaaggcgt ttccagccaa aaattagcgg ctctgttaga agagcaaggc
     7321 attgtcaccc acgatgacgc cgatcttatc ccttatttaa tgcgattata ccctgagtta
     7381 agcaaattca aggctggtgc ttattcactc gcaggcttaa cgacagtaaa agatttgctc
     7441 gctcatttaa gctccggcaa agaagtgcaa cttaacgtgc aatttattga gggcaaaact
     7501 tttaaaattt ggcgggaaca actagcgaaa gccgattatt tacagcacac gctacaaggc
     7561 aaatcggaaa aagaaatcgc tgacttactc ggcattccac acgaaaaaat cgaaggttgg
     7621 atagccccgg atacttacaa ctacacgccc aattccaccg atttagcctt gcttaaacgg
     7681 gcttatcaaa agcagaaaac ggcgttggaa caggcttgga aaaatagagc ggaaaatcta
     7741 ccgcttgcaa caccatacga aatgctgatt cttgcctcga ttgtggaaaa agagaccggc
     7801 attgcgaccg aacgcccaca agtggcatcg gtctttatta accgtttacg caacaatatg
     7861 cgactgcaaa ccgatccaac cgtcatttac ggaatgggcg atcgttataa cggcaatatc
     7921 cgccgcaaag atttagaaga ggcaacgcca tacaatactt accagattga cggcttaccg
     7981 ccaacgccga ttgccatgcc gagtgaagct tcgattaaag cggttgccaa cccggataat
     8041 acaccctacc tctattttgt ggcagacggc acgggcggtc ataaattcag caaaacactg
     8101 aatgagcata ataaagcggt ggcagaatgg attcaaattg aacgcaaacg caaaaatgaa
     8161 caaaaatagt gagaacaata tgcgtggaaa atttattgta attgaaggct tagagggcgc
     8221 aggcaaaacg actgctcacc aagtgattct caaagagttg gaacaagccg gcattaatga
     8281 cgtagtaatg acccgagaac ccggcggcac cccccttgcc gaaaagttac gccatttaat
     8341 taaacacgaa accgaagaag ccattaacga taaggctgag ctgctaatgc tctacgctgc
     8401 tcgtgttcaa ttagtcgaaa atgtgatcaa acctgccctt gccgaaggca aatgggttct
     8461 gggcgaccgc cacgatatgt cttcccaagc ctatcaaggc ggtggacgta agttggatcg
     8521 tcacttactc gaaaccttaa aagaatcggt actcggcaca ttcgagccag atttaacgat
     8581 ttatctcgat ctcgatccgg tgattggttt agaaagagcc agaggcagag gcgaattaga
     8641 ccgcatcgaa cagcaaagcc ttgacttctt ctaccgcaca cgagagcgtt atttagaatt
     8701 aacccaacac aacgataaag ccgtgatcat caatgccgaa caacccatcg aacaggtgac
     8761 tgcggatatt caacaagtaa ttagaaactg gctgaaataa tgtacccttg gctcgaaagc
     8821 acttatcaac aacttaccga ttcattttta cagggtcgag gtcatcacgc cttactgttc
     8881 aaaacagatg ttggtcttgg tacagacacc ttaatctaca aggtggctga atggttgctt
     8941 tgcagccaaa aactccgcca tcaaccttgt cttgaatgca aaagttgcct gcttatgcaa
     9001 agcaacaacc accccgattt ccacattctt gagccgattg aagacaaaga tattggtatc
     9061 gaccaagtgc gtgagttgat ttataaactc cagaatttcg cccaacaagg gggaaatacg
     9121 gtcgtttata ttcgaggagt tgatcgttta accgaggcaa gcagtaatgc cctgctcaaa
     9181 accttggaag agccacgtga aaacgtctat ttcttgctcg aagcaccgct tcaagcaaca
     9241 gttttagcta ccattcaaag ccgcagccaa acttgggtac tttacgcccc acaagcctca
     9301 gaagtaatgg cttggctaca aacacaattt ccggcaatct caaccgacga cttagagatt
     9361 gcactccgcc tttgccactc acgcccacta atttgcaaaa attttatcga aaatgaccgc
     9421 ttgcaacagc gaaaaacctt tttgcaaacc ttctggcgtt tctacaaaag ccacgatgtc
     9481 ttcttgttat tccaagcctt cgcccaagaa aaagagctga ttttacagca actcgaatgg
     9541 ctggaaagtt tctttagcga tgccctaaaa gctaaaatgg caatcagcca cggttggcta
     9601 aacccggatc tccaaaacgg cattctccct tttagccaac aactaacgac ccacggctta
     9661 cttaaaggac atcaaattct ccaacaaacc cagcgggatt taaccgaaat taacgccgtc
     9721 aatccggaat tgatgttgct agattgcctg acgaagttgg ttttggtgtt tgagtaaaag
     9781 ccctctctca tagaaggtag ctttcaatta gccgtaaaaa gaggcttcta gccctagaat
     9841 atagtttata acttgcaaaa ttatctatct ttgttattgt atgaataatt cacacaagga
     9901 gaatattatg gcaagaacaa ccagcgtgac tttaggcgaa cctttaaatg attttgtaaa
     9961 tgaaatggtg gcaagtgggc gttatggctc taccagtgaa gtagtgagaa ccgctttacg
    10021 aatgcttgag gaaaaagagc aatacttagc tcagttgcga caagccattg atgatggtga
    10081 tgctagtggt tatgcaaccg aaacgattga tgaaatgatg ttgagagtaa aggcggaact
    10141 taatgtataa actttctgct caagcactcc gagatcaaga gaaacttttt cgttacagta
    10201 tctctcaatt tggtgttcaa caaacagaat cttattttgc agatctaaag catattttga
    10261 atttattagc tgattttcca acaatgggaa cttctgcgga atatattcgt ggtggcttgc
    10321 gaatgcacac acatcaatct catacaattt attatcgaat aaaagcagat catatattta
    10381 ttgttcgcat cttacataac caaatgaatc ataaacgcta tatttagaaa aatgaaagac
    10441 ttatttatca tagactcaca ttgtcatcta gattctctag actacgaaac tcgtcacaaa
    10501 aacgtggacg aagtaattga aaatgccaaa gcccgagggg tgcatcattt tatctcggtt
    10561 tgcactacag ttgggcgttt tgaagcaatg aaaaacttaa ccgcccatcg taatgacgtt
    10621 tcactttctt gtggcgttca cccgttgaat gtggaagatg aaccttttga ttacgataaa
    10681 ttatttcagt tcgctcaaga tccgaaagtg gttgccattg gggaaactgg tttggattat
    10741 cactacacac cggaaaccaa agcgttgcaa caatcgcttt tcgttcaaca aattgaaatt
    10801 gcaaacaaac tcaataagcc actcattatc cattctcgtt cggcacgcca agatacgatg
    10861 gatatgttgg aacaaaataa tgccgaaaaa tgcggtgggg tgttgcattg ctttaccgaa
    10921 gattgggcaa tggcaaaacg ggctttagac atcggctttt atatctcaat ttcgggcatt
    10981 attactttcc gcaatgcaga agaactccga gaagtcgtgc gaaaagtgcc gttagatcgc
    11041 ttattggttg aaacagattc cccttatctt gccccgattc cttatcgtgg taagccgaac
    11101 cagccggcgt atgtgcggga aacttgcgaa tatgtggcga cattaaaagg cgtttcaact
    11161 gaagaattag ctcgcattac aaccgaaaat gtgcaacact tattcaacat taaattataa
    11221 ggaactaata tgagagcaat acttaagtat tttctgattc ttgtctcact atcatttttt
    11281 attgtattag gcggtgcagt taattatgca atgccaagtt atgaagatac cgtagtaaca
    11341 ggtatggaag tacgccgaat ggataaagat ggtattatca gcaaagcgaa tcctgctgat
    11401 ggcgaggtgc gcgatgttta tttcttattc acggaagatc ctgaaaccaa aaaggtaatg
    11461 gtttatcgta atgaagatac cggctggggg ttgcctcctt atttcaaatt cggatctgcc
    11521 gacattcaag ccaaagctca agcctacgct aacgaaaaac aacgtgtgca aattaaatat
    11581 tacggctggc gaatcaattg gctaaatgaa tttagaaata ttgtttcaat caagccatta
    11641 gaagaagcag aaacagtcag caaaccaatt atgagctatg tactttatgc cgtattggcg
    11701 ttcttgttct tcctctcagt acaatttatt cgtggcattt ttaaagatta atgccgatta
    11761 acctttgaca aacggttcga ttttgtaaaa aattgaaccg ttttcttttt tatcaacaat
    11821 atcaaatgac gtactatttt attgccgatc tccacttaaa tgaaaatcag cctgaaatta
    11881 cccacctctt tttgcaattt atgcaagaaa aagcactact tgcagaagcg gtttatatct
    11941 taggtgatct gtttgatttc tggattggcg atgatgagca gtctgaactc atttcaaccg
    12001 ttaaacaggc aatcaaaacc ctcactacaa gcggtacaaa atgctatttt atttgcggaa
    12061 atcgtgattt tttaattggc aaacgattca gcgaacaaac gggtatggtt attttgcctg
    12121 attatcacgt tatagattta ttcggcacat ctaccttgct ttgccacggc gatacgcttt
    12181 gtactgatga tgtgaaatat caacaatttc gtaaaaaagt ccaccaaaaa tggcgacaag
    12241 ctatcttttt gatgtttccg ctttcttggc gaattcgtat tgcggagaaa attcgatcca
    12301 aaagcaaaca agaaaagcgt gataaatccg ctgaaattat ggacgtaaac gctgaagtta
    12361 ccgctcaaat tgtcgcaaaa tacaatacaa ctcaacttat acacgggcat acgcatcgac
    12421 aagcaatcca tcagcataat ggatttaccc gcattgtatt aggcgactgg aaagaaaatt
    12481 acgcctctat tttaagcgta accgagcaag gagctgaatt tatatgatta atgtgattat
    12541 tccttgctat aacgccgaaa acacccttgt tcgtgcagtg caaagtgtat taaatcagtc
    12601 tggattaaat ctattgtggc ttattgatga tgcttcaacg gataatacgc ttgccttagc
    12661 taaacattta caagcccaag tgcctgataa aattagagtg gaacaaatgc ctcaaaatgg
    12721 cggtgttgcc aaagcacgca actggggagc attacaaagt gaaacagatt taattgcctt
    12781 tttagatgct gatgatgctt atgaaaacgg ggcattgcaa gttgctgaaa agatttttga
    12841 gtttaaaccg gaaaccggct tggttcggtt agcattaaaa ccggtaactt agatcaacgt
    12901 tacagctctc accctaattt tgagttagct tggcaatata tgcaaatgac tggtgcaggc
    12961 aacactgtat ttcggcgtag tttcttcctc gcttgtggcg gctttccaca acatcagctc
    13021 ttccaagaat taggtggaga agatggtgct ttaggcatag ccactacaca aattagctct
    13081 gtagctactg catttaatga tgtcggtgtt ttacattact gccgagaggg tatgcacgca
    13141 gaaagattat taaatgcgat tttattcaac gaaaaagatc ccaatgttac tgaagaaaaa
    13201 gttaaacaag ccaacctaat taccgaaaat attgttaata atattagaaa tctacaagac
    13261 tgtcttaata gtaaagggat tggtattaag ccttatacgc ttgaatggag ctaaaaaagc
    13321 atttgcagat tttccctaaa aaaa
//
LOCUS       DANLXQ010000061        13242 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.061, whole genome shotgun sequence.
ACCESSION   DANLXQ010000061 DANLXQ010000000
VERSION     DANLXQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.061"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(157..1506)
                     /locus_tag="PXD94_001349"
     CDS             complement(157..1506)
                     /locus_tag="PXD94_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL6286213.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKERAPFALG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVAQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETAIGLTGFACALIGSWLL"
     gene            1693..2208
                     /locus_tag="PXD94_001350"
     CDS             1693..2208
                     /locus_tag="PXD94_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL6286214.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFELVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCVKAVEPLL"
     gene            complement(2262..3261)
                     /gene="gntR"
                     /locus_tag="PXD94_001351"
                     /pseudo
     CDS             complement(2262..3261)
                     /gene="gntR"
                     /locus_tag="PXD94_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674159.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
     gene            complement(3354..4514)
                     /locus_tag="PXD94_001352"
     CDS             complement(3354..4514)
                     /locus_tag="PXD94_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL6286215.1"
                     /translation="MIEQSYKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDD
                     FVYHKNSIESLSIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNPDYYPDLPYVATD
                     NYALVEAAFLHLKQKGINQFAFYGYPAETEKHWAIERKNAFIQLMEKYEHHPNCYIGD
                     QFHSDNWVELQAKLGEWLQTLPAHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGI
                     DNEELIQYLSRVSLSSVTQGTSQIGYHAVKLLYRKLNNLPVSTKPILIPPVKVEERRS
                     TDYRSLQDPMVIQAMHFIRHRACQGIKVEQVLDHLKISRSNLEQRFKQEMNKTIHQVI
                     HEEKLARATYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            complement(4637..5023)
                     /gene="rplQ"
                     /locus_tag="PXD94_001353"
     CDS             complement(4637..5023)
                     /gene="rplQ"
                     /locus_tag="PXD94_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL6286216.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPEVATEAAAE"
     gene            complement(5059..6048)
                     /gene="rpoA"
                     /locus_tag="PXD94_001354"
     CDS             complement(5059..6048)
                     /gene="rpoA"
                     /locus_tag="PXD94_001354"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL6286217.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(6079..6705)
                     /gene="rpsD"
                     /locus_tag="PXD94_001355"
     CDS             complement(6079..6705)
                     /gene="rpsD"
                     /locus_tag="PXD94_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL6286218.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(6734..7123)
                     /gene="rpsK"
                     /locus_tag="PXD94_001356"
     CDS             complement(6734..7123)
                     /gene="rpsK"
                     /locus_tag="PXD94_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL6286219.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(7140..7496)
                     /gene="rpsM"
                     /locus_tag="PXD94_001357"
     CDS             complement(7140..7496)
                     /gene="rpsM"
                     /locus_tag="PXD94_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL6286220.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(7634..7747)
                     /gene="rpmJ"
                     /locus_tag="PXD94_001358"
     CDS             complement(7634..7747)
                     /gene="rpmJ"
                     /locus_tag="PXD94_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL6286221.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(7772..9091)
                     /gene="secY"
                     /locus_tag="PXD94_001359"
     CDS             complement(7772..9091)
                     /gene="secY"
                     /locus_tag="PXD94_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL6286222.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFTGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(9094..9528)
                     /gene="rplO"
                     /locus_tag="PXD94_001360"
     CDS             complement(9094..9528)
                     /gene="rplO"
                     /locus_tag="PXD94_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL6286223.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(9533..9709)
                     /gene="rpmD"
                     /locus_tag="PXD94_001361"
     CDS             complement(9533..9709)
                     /gene="rpmD"
                     /locus_tag="PXD94_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL6286224.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(9716..10216)
                     /gene="rpsE"
                     /locus_tag="PXD94_001362"
     CDS             complement(9716..10216)
                     /gene="rpsE"
                     /locus_tag="PXD94_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL6286225.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGHGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(10231..10584)
                     /gene="rplR"
                     /locus_tag="PXD94_001363"
     CDS             complement(10231..10584)
                     /gene="rplR"
                     /locus_tag="PXD94_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL6286226.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(10599..11132)
                     /gene="rplF"
                     /locus_tag="PXD94_001364"
     CDS             complement(10599..11132)
                     /gene="rplF"
                     /locus_tag="PXD94_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL6286227.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(11149..11541)
                     /gene="rpsH"
                     /locus_tag="PXD94_001365"
     CDS             complement(11149..11541)
                     /gene="rpsH"
                     /locus_tag="PXD94_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL6286228.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(11578..11883)
                     /gene="rpsN"
                     /locus_tag="PXD94_001366"
     CDS             complement(11578..11883)
                     /gene="rpsN"
                     /locus_tag="PXD94_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL6286229.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPIRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(11895..12434)
                     /gene="rplE"
                     /locus_tag="PXD94_001367"
     CDS             complement(11895..12434)
                     /gene="rplE"
                     /locus_tag="PXD94_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL6286230.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(12452..12763)
                     /gene="rplX"
                     /locus_tag="PXD94_001368"
     CDS             complement(12452..12763)
                     /gene="rplX"
                     /locus_tag="PXD94_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL6286231.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNPETNKADRVGFRFEDGKKVRFFKSNEKT
                     I"
     gene            complement(12774..13145)
                     /gene="rplN"
                     /locus_tag="PXD94_001369"
     CDS             complement(12774..13145)
                     /gene="rplN"
                     /locus_tag="PXD94_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL6286232.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
BASE COUNT         3809 a         2947 c         2599 g         3887 t
ORIGIN      
        1 gcagcagaaa ggttttggaa taatttacaa aatattcagt ttataaatga agatatatta
       61 aataaaccct aacattctat aataagaaaa agcggtcgat tctctgccaa attttgcaaa
      121 ttttagaaga aaacagaccg cttgtcaggg ttggaattag agtaaccagc tgccaatcaa
      181 tgcacaggca aaaccggtta aaccgatggc agtttcaagc attgtccacg ttttgagggt
      241 agttttttcg tccatttcaa ggaaacggct catcagccag aaaccggagt cattgacgtg
      301 ggaaagcacc gtcgcaccgg aggcgatagc aatcacgata aaacagaggt cgaactggct
      361 taaatcagtc gctgccgcca cagttggcgc aatcaacgct gctgtggtgg ttaaagcaac
      421 ggttgccgag ccttgtgcga cacggaatac cgtagcaata ataaaggcgg caaccacaat
      481 cggcattccg gtgtctttca tcatttccga taatacatcg ccaatcccgc tggcacgcaa
      541 cacgccacca aacattccgc ctgcaccggt tactaagaca atcgagcaga ttgggccaag
      601 ggcattgtta cagatttttt cgatttggtc gaaactgcga ttatcccgca acagcataat
      661 cgccacaatt aaggtaatca gtaaagcaac aggggttttg ccgattaagc gaaggctctg
      721 cacccacatt tcactgccat caatcacttt agcaacggag agggtattca agccggtatc
      781 aaacaaaatc aggataatcg gcagcaccaa caccgtgagt accttgccaa aacttggcgg
      841 gttttgtact gccatttcgt ttgccgataa gccgtttaaa aacgctttcg gcaaatcaac
      901 gtgaatgcgt ttgctgataa attggctgaa caggtaagtg ccgatatacc acgttgggat
      961 tgcacaaacc aagcccacca agactaacag ccccatattc acaccaagca gatcgccgga
     1021 ggcaaccggg ccggggtgcg gaggtagaaa ggcgtgcatt accgcaaacg caccggcaga
     1081 cggaaacgca tagcgcaaca ccgaaccgcc gaactgtttt gccacgctaa aaataatcgg
     1141 taacatcacc accaagccgg catcaaagaa aatcgggaag ccgaataaca gcgaggcaac
     1201 gcccaacgca aaaggcgcac gttctttgcc gaatttattg ataagcgtat ccgcaagcac
     1261 tttcgccccg ccggtgattt caagcaagcg accaatcatc gccccaagcc ccaccagtaa
     1321 cgccactgat gccagcgtac taccaaaacc attcaacaat gttggcagga ttttatctac
     1381 cggaataccg gcagctaatg cagtgagcag gctcacaata atcaatgcca caaaagcgtg
     1441 gactttaaat ttcataatca gcaccagcaa taaaatgatg gcagcaaaca taatgaacag
     1501 taacatagga tctcctaagg ttgaattgtt atcggtaaca taaccaaaaa gcagtttaga
     1561 gtaaacggtt gcagggcttt tattttaaaa atgtgatcta cttcaagttt tctggaaata
     1621 gcagatgatt tttcagcata acctgttaca ttaacggcta agttaacgtt accagtaaca
     1681 caaggaaatt caatggcaaa cggcaaagca tttattttga tgggcgtgtc cagcaccggc
     1741 aaaaccagcg tgggaacggc agtcgcacag cggttgggaa taaaattgat tgatggggac
     1801 gacttacacc ctcgtgccaa tatactgaaa atggcaagtg gcacgccact caatgatgca
     1861 gatcgcacac cgtggttgga gcggattaac gatgcagtgt tcagttttga acaaaaaagc
     1921 gaaaaaggga ttatcgtctg ctcagcattg aagaaaaagt accgagacca gatccgacaa
     1981 ggcaatgccg atgtgacttt tctattcttg cacggctcgt ttgaactggt gctggctcga
     2041 atgcaacaac gcaaagggca ttttatgaaa ccggaaatgc ttcaaagcca atttaatacg
     2101 ctggaaattc cgcaggcaga cgaaccagat gtgatattta ttgatattga cggcagtttt
     2161 gacgaggtgg tggagcgttg cgtgaaagca gttgagccgt tgctttaatc acaagtggtg
     2221 gattttgcgg atttttttgc aaatttaacc gcttgtccgg gctacacact ttcccccaaa
     2281 tggatctgat agcccaagtt aattatggag ctttgttgtg gtacgccgtg aatacggttg
     2341 agcagctctt gggcggcaac tctgcctatt tcaaggcgag gtgtgatgac gctggcaagc
     2401 tgtggcgtga gggattgccc gacatcgtgt ccgtgaaagc cggcgatttt aatctgctgc
     2461 ggcacatgaa tgcctaaccg ttggcactca aataatgccc cgattgccaa atcgtcattg
     2521 gtgcaaaaaa tgccgtctag ctgcggaatg tgttgcaggg cttgttttag ctggcttgcg
     2581 ccaagcgtga aagaagaatg ctctgcagta gtaatgctgt gtgggcttaa gccgtgtttg
     2641 agcattgcct gttcgtagcc ctgcattttc agctgagagc gtttatccat tctcgccccg
     2701 aaatacacca cctgtcggca gcctcgatga atcattgtct cgaccattgc ttgagccgca
     2761 gaaatattgt caatatccga ccgcttgttg aatgccacat tcgccgcttt ccattatctc
     2821 aatcaccgga atattcgcca cttccagcat tttcaaggtg cggggcgagt ggtgattttc
     2881 cgataagata atgccgtcaa tattgtacga aagcagcgac tcaatccgct gctcttcctt
     2941 ttgttcgctg taaccatagt gggcgagcat ggtttgatag ctggcttggt cggtaatctg
     3001 ctcaatgcct ttaatcacat cggcaaacac ttgattggta agcgaaggaa ccagcacgcc
     3061 aattgcacgg cttttggcat tcgagagaat atccggtgca cggtttggga tatagccaaa
     3121 cagctcaatc gcttgtgcaa tccgctcgcc ggtctccttg gcaacggtgt ttggatcccg
     3181 caaaaagcga ctgaccgtca ttttggtaat gccgagatgg gtagcgatgt cttgcaaagt
     3241 tgggcgtttc ggttttctca ttggaataga taacgaaaaa atttaatttc gctgattata
     3301 actgaagata aggcaataat tgcaaaatat tcggtgaaat tgaccgcttg ttactactct
     3361 tgcctgaact ctttcggggt tttaccaaac tcttttttaa acacggcata gaaatattgt
     3421 aaagagggat aaccgcacac attggcaatt tcttgggtcg aaatctcggt aaatttgagc
     3481 atatatgttg ctcttgctaa tttttcctcg tgaatcacct ggtgaattgt cttattcatt
     3541 tcctgcttaa aacgctgttc aaggttagag cgggaaattt taagatgatc cagtacttgc
     3601 tctactttaa tgccttggca agctcggtga cgaataaagt gcatagcttg gattaccatt
     3661 gggtcttgca aagaacgata atcggtcgaa cggcgttctt ccacttttac cggcgggatt
     3721 aaaatcggct tggttgaaac cggtagatta tttaattttc gatataataa cttcacagcg
     3781 tgatagccga tttgtgatgt tccttgtgtg actgaagaaa gagaaacccg agagaggtat
     3841 tgaatcaact cttcgttatc aatgccgatc acgcaaagtt gttcaggtac agcgattttt
     3901 aaatgctcac aggtttgaag caggtgtctt gcccttgcgt cagtaacggc aataatgccc
     3961 gtgtgggctg gtaaggtttg taaccactcg cctaatttgg cttgtaattc aacccaatta
     4021 tctgaatgaa attgatcacc aatataacaa tttgggtgat gttcatattt ttccatcaac
     4081 tgaataaagg cattttttcg ctctatcgcc caatgttttt ccgtttctgc aggatagccg
     4141 taaaacgcaa attggttgat tcctttttgt tttaggtgta aaaatgctgc ctcaactaag
     4201 gcgtaattgt cggtagcaac ataaggaagg tcagggtaat agtcagggtt ttggtaagaa
     4261 ccgcctactg ctacaatggg gacgtcaata tctttaagta gctcaactgt ttccaaatcg
     4321 tcaaaatcgg caataatgcc gtcaatcgac aggctttcga tactattttt atgatagaca
     4381 aaatcatctt ccacaaaaat atcccaagta cattgtgatg cttgaataaa ctgcccaatg
     4441 ccctgcatta ctcctctgtc ataaattttg ttagcattaa aaagtaaggc aattttataa
     4501 gattgttcta tcattacttc ctccagtacc cactttcaaa agtaactata aaataaaatt
     4561 attttttgat caaaacgaat aaaagaataa aaccccggta tttctaccag ggttttaagc
     4621 tcaattttga ttaagattat tccgcagctg cttcagttgc aacttctggg cgatccacta
     4681 actcaatgta agccattgga gcattatcac ctgcacggaa accacatttt aaaatgcgag
     4741 tgtaaccacc cgcacgttgt gcaaaacgtg gacctaattc attgaataat ttagcaactg
     4801 tatcaacgtt gcgtgtacgg gcaaaagcta aacggcgatt tgcaacgctg tctgctttag
     4861 ctaaagtaat taacggctca actacacggc gtaactcttt cgcttttggt aaagttgtct
     4921 tgatgatttc gtgttcaaca agcgcacttg ccatattacg gaacatcgct tggcgatggc
     4981 tgctgttacg gtttaattga cgtccactct tacgatggcg cataacctat tccttcttag
     5041 aaaatctata ccgaaaaact agtcttctgc aatacttgca ggtggccagt tttcaaggcg
     5101 cataccgagt gataaaccac gagaagcaag aacatcctta atctctgtaa gagatttttt
     5161 acccaagttc ggggttttaa gaagttcaac ttctgtacgt tgtactaaat caccgatata
     5221 gtgaattgtc tctgctttca aacagttagc agaacgaact gtcagctcta ggtcatctac
     5281 tggacgaaga agaataggat caaattccgg tttttcttct ttcacttcag gttgacgaac
     5341 atcacgcaaa tcaacgaatg cagcaagttg ttctgctaaa atcgttgcag cacgacggat
     5401 agcttcttca ggatctaacg ttccgttagt ttccatctca ataacaagtt tatctaagtc
     5461 tgtacgttgt tcaacacgag cagcttcgac attgtaagca atgcgatcta caggtgaaaa
     5521 acgtgcatct actaataaac gaccgatagg gcggtcatcg ttttgagtat gaagacgtgc
     5581 tgatgcagga acataacctc gaccacgttg aacacgaatg cgcatattaa ttgatgcatt
     5641 cttatctgtt aaatgacaga ttacgtgatc aggattcaca atttctacat cgccatcgtg
     5701 tgtgatgtcg gctgcagtta cagggccaat tccagattta tttagtgtca aaataatatc
     5761 atctttattt tgcactttta ccgctagacc tttaaggttt aataatacct caagaatatc
     5821 ttcctgaacg ccttctttgc tgctgtactc atgcagtaca ccatcaattt caacttcagt
     5881 aactgcacaa cccggcatag atgaaagtaa aatgcggcga agtgcgttac ctaatgtatg
     5941 gccaaaacca cgctctaacg gctctaatgt cactttagca tgagttgaac taacttgttc
     6001 aatatttact aagtgcggct ttaaaaattc tgtcacagaa ccctgcattt tttcctctct
     6061 ttgcggttaa gctaactatt atttagagta aagctcaacg atcagatgtt cattaatatc
     6121 tgctgataga tcagaacgct ctggacgacg tttaaatacg ccttccattt tagttgcatc
     6181 aacttctaac caagttggtt tttcacgttg agttgctaat tctaatgatg ctttaatacg
     6241 tgcttgtttt ttcgattttt cacgaacagc aacaacatca tcaacagaaa cttggaatga
     6301 aggaatgttt actacacgac cgtttactac gattgattta tggctaacaa gctgacgtgc
     6361 ttctgcacgt gttgctgcaa aacccatacg ataaactacg ttatctaaac gaccttccaa
     6421 taatactagt aagttttcac cggtattacc ttttaagcgg ttcgcttctg tataataatt
     6481 acggaattga cgctctaaga taccataaat acggcgaact ttttgttttt cacgtaattg
     6541 actaccatag tcagataaac gtggcttgcg agcaccatgt tgaccaggcg ctacatcaag
     6601 tctatttcta cattttgatt caatcgcacg aacgcctgat tttaagaata aatcagtacc
     6661 ttcacgacgg cttagcttga gcttaggacc caaatatctt gccattttct ttctccaatt
     6721 cttttaacgt caattaaaca cgacgttttt tcggtggacg acaaccgtta tgtggaatcg
     6781 gagtcacatc agtaatattc gtgatacgga aacctgctgc atttaatgca cggattgttg
     6841 actcacgacc tggacccggt cctttaacca taacttccaa attctttaaa ccaaactctt
     6901 taaccatttc tgcacaacgc tctgcagcaa cttgagcagc gaatggagtc gatttacgag
     6961 aaccacggaa acctgaacca cctgcagttg cccaagctag agcattacct tgacggtcag
     7021 taatagtaac gattgtgtta ttgaaagatg cgtggatatg agctacgcca tctgcaatct
     7081 gtttttttac gcgcttacgt gcgcgaactg gtgttttagc cattatttat ctactccgac
     7141 tattttttga tcggtttgcg tggaccttta cgggtacgcg cattagtctt agtacgttga
     7201 ccacgtaccg gtaaactacg acgatgacgt aaaccacggt agcaacctaa gtctaaaaga
     7261 cgtttgatgc ttaaagttac ttcacgacgt aaatcccctt cgacagtaaa tttaccaact
     7321 tcttcacgca gtttttcaat ctgctcttca gacaattctc tgatctttac atcttcagca
     7381 atacccgttg cagcacagat ggcttttgca cgagttttac cgataccgta aattgcagtt
     7441 aatgcgatta cagtgtgttt ttgatcagga atgttaatgc ctgcaatacg ggccactatg
     7501 cactcctatt atttaagttt attgatatac tgatcttgaa aagcccgttt caggatactc
     7561 aaacagtata tcaggacgaa tgagctgagc agtataactg ctcagcgggt tctttgcaag
     7621 aaatatgcgt aaattaacct tgacgttgtt tgtgtttagg atcgctacaa attacacgaa
     7681 ccacaccttc acgtttgatt actttacaat tacggcacat tctttttact gaagcacgaa
     7741 ctttcattgc ttatccttta ttaacggcct attaatttag accttttaag ttagctttct
     7801 tcaacacaga atcatattgt tgagacatta aatgactctg aacctgtgcg atgaaatcca
     7861 taataacaac cactacaatc aataatgatg taccacctaa ttggaatggc accttccata
     7921 acgacgtaat gatgtaagga accaaacaaa caaaagcaat atataacgca ccaattaagg
     7981 ttaaacgtgt cattacttta tcgatataac gagatgtttg ttcgcctggt cggtaacctg
     8041 gtacaaacgc acctgatttc ttaagattat ccgctgtatc acgaggatta tactgcattc
     8101 ctgtataaaa gaaagcaaag aaaatcactg ccattgtgaa taatacaaca tacagtggtt
     8161 gacctggctg taatagttgt gaaaggttaa ataaccactc aaatccttcg ctttgaccaa
     8221 accattgagt aatgcttgcc gggaataaaa taatacttga agcaaaaatt gccggtatta
     8281 cccctgccat attcactttt aatggtaagt gagaagttct tgcaggcgcc atcattcgac
     8341 cttgttggcg acttgcatag tttacaacta ttcttctttg tccacgttca acaaaaacaa
     8401 caaaataagt tactgcaaat gcaattattg caactagtag taagacgata aatgataact
     8461 cgccttgacg ggcttgttct attgtctgcc ctatcgttgc tggaagatct gcaacaatac
     8521 ctgtaaaaat aatcaatgag ataccgtttc cgataccacg ctcagtgatt tgttcgccta
     8581 gccacattag gaacattgtt cctgtcacta agcttactac agaggttaca tagaataata
     8641 tacttggatt aggaactaat ccatgtacca tattcggtag ggctagtgaa ataccaatag
     8701 attgaataaa tgctaataac agtgtgccat agcgtgtata tttactgatt ttccgacgac
     8761 ctgcttcacc ttctttcttt aattcagcta gaggtgggtg aatcgctgtc aataattgga
     8821 taataattga cgctgaaata taaggcataa tacctagtgc aaatatagat gcacggctta
     8881 aagcaccacc agagaacata ttaaacatat caatgatggt gccttgctgt tgtcgaatta
     8941 attcggataa tacagaagca tcaatgccag gaacaggtat aaaagaaccg atacggaaaa
     9001 cgattaacgc acctaaaaca aacaatagtc ttgatttaag ctcgccagtc cctttttgtg
     9061 tactaccttg gtaccctggt tgctttgcca tctttattcc tcgatagaac cgccagcagc
     9121 ttcaatagca gctttagcgc ctttagttac acgtaagcct ttaacaacta ctgctctttc
     9181 aattttgcct gctaaaatta ctttagcaga taaaatatct ttagtaatga tgtttgctgc
     9241 ttttaatgtc tctaaagtta cttcgttgcc atctacttta gctaagtcgt ttaaacgaat
     9301 ttcagccaca tggaatgatt ttaatgaagt aaaaccaaat tttggcaaac gacgatataa
     9361 aggcatttga ccaccctcga aaccacgacg aacaccgccg cctgtacgag atttttgacc
     9421 tttatgaccg cgcccaccag ttttacctaa accagaacca ataccacgac ctagacgttt
     9481 tgcactgtgc ttagcacctt cagctggaga aagagaattt aaacgcattc tattactcct
     9541 ctactttaac catataagac acttgattga tcataccacg cactgctggt gtatcttcaa
     9601 gttctacagt atgacgaata tgacgaagac ctaagccttt taacgttgct ttatgtttcg
     9661 gtaaacgagc gatagagcta cgagtttgag ttactttaat ttttgccata tccaattacc
     9721 ccaaaatttc ttcaactgtt ttgccacgtt ttgcagcaac catttcaggt gatttcatgt
     9781 tctcaagtgc atcgattgtt gcacgaacaa cgttaattgg gttagttgaa ccatacgctt
     9841 tagaaagaac gttacgaaca cccgcaactt ctaatactgc acgcattgca ccaccagcga
     9901 tgatacctgt accttcactt gctggttgca taaatacgcg tgaacctgta tgtgaacctt
     9961 taactggatg ttgtaatgta ccttcattta aagctacatt aatcatattg cgacgagctt
    10021 tttccatcgc tttttggatt gctgctggaa cttcacgtgc tttaccgtga ccaaaaccta
    10081 cacgaccgtt accatcgccc actactgtta aagcagtgaa actcataata cgaccacctt
    10141 ttacggtttt tgaaacacgg ttaaccgcga ttagcttttc ctgcagttca ccagcttgtt
    10201 tttcgatgtt tgacatctca aattcctcta ttagaactgt agaccagctt cacgagcagc
    10261 atctgctaat gattgcacac gaccgtggta tttaaaacca gaacggtcaa aagcaaccgc
    10321 tttaatacct ttggcaattg cacgctcagc aacgatttta cccactactg ctgcggcttc
    10381 tttattgccg gtatatttaa cttgctcttt aattactttt tcaacagttg aagctgctgc
    10441 tagtacttct gagccattag gtgcaatcac ctgcgcatag atatggcgag gtgtgcgatg
    10501 cacaactaaa cgtgttgcac cttgctctct cattaaatga cgtgcacggc ttgcacgacg
    10561 gatacgagct actttcttat ccatagtgtt accttacttt actttttctt cgcttctttt
    10621 gtacgcacta cttcatcaga gtaacgtaca cctttacctt tataaggttc aggtttgcga
    10681 tatgcacgaa tatctgctgc cacttgacca attaactgtt tgtcagcact tttcagaata
    10741 atttctgttt gtgatggaca ttcaccagtt acgcctgctg gcaacgtgtg ttctactggg
    10801 tgagagaaac ctaaacttaa agcaacagca ttgcctttca tttgtgctct ataaccaaca
    10861 cccactaatt gcaatttctt agtaaagcct tcagtaaccc cgattaccat attatttact
    10921 agtgcacgag cagtacctgc ttgagcatct gcattagcga taccttcacg tggagcgaaa
    10981 gttaatgcat ctgcttcttg ttttacttca actgcattgt gaatttcacg agataactcg
    11041 ccgtttttac ctttaactgt taatagctga ccgttaagtt ttacctcaac accggcagga
    11101 atattaacag gtgcttttgc aacacgagac atttttccta cctctctatt atgctacgta
    11161 acagataatt tcaccgccga ggcccgcttg acgcgcagca cggtcggtca ttacaccttt
    11221 agatgtagaa acaactgcga tacctaaacc acccattact tttggtaatt cgtctttgcg
    11281 tttataaata cgcagacccg gacggctcac acgttggata ctttctacaa ccggtttgtt
    11341 ttggaaatat tttaaagtaa tttccagttc aggtttaaca ccttcaacaa ctttaacgct
    11401 ttcgatataa ccttcttcag ctaaaacatt tgcaatagca acttttaact tagatgaagg
    11461 catactgatt gcaactttgt tcgcagcttg accgttacga atacgggtca gcatatctgc
    11521 gattggatct tgcatgctca tgtatttact cccatgattc caaattaaaa gaggttatta
    11581 ccagcttgcc ttcttaagac ccggaatttc accgcgcata gcagcttcac gaaccttaat
    11641 acggcttaaa ccaaacttac gaagtacgcc atgtggacga ccagtttggc ggcaacgatt
    11701 acgctgacgg attgggctag aatcacgagg aagtgtttgt aattttaaca ctgctgccca
    11761 acggtcttca tcagatgcat tcacatcaga gatgattttc tttaattctt cacgttttgc
    11821 gtagaattta tcagctaatt tagcacgttt tacatcgcgt gcgatcattg attgttttgc
    11881 cacgataacc taccttattt acggaatggg aaattgaaag cagctaataa agcttgacct
    11941 tcttcatcag ttttcgctga ggtggtgata gtaatatcta aaccacgaac acgatctact
    12001 ttatcgtagt cgatttcagg gaaaatgatt tgttcacgaa cacccatact gtaattacca
    12061 cgaccatcga atgacttagc gtttaaaccg cggaagtcac gaatacgtgg aacagcgata
    12121 gtaatcaatc tttcaaagaa ttcccacata cgttcaccac gtagggttac tttgcaaccg
    12181 attggatacc cttgacggat tttaaagcct gcaacagatt tgcgagcttt agttactaaa
    12241 ggtttttgac cgctgattgc tgctaaatcc gctactgcgt tatctaacag ttttttgtcg
    12301 gtcaatgctt cacccacacc catgttaagg gtaatctttt cgattcgtgg gacttgcatg
    12361 acagatgagt agttgaattt tgcttttaat tcattaacta ctgtatctct gtagtaatca
    12421 tgcagtttcg ccatcacatt actccagtta attaaattgt tttctcgtta gatttgaaga
    12481 aacgcacttt tttgccatct tcgaatctaa atcctacacg gtcagctttg ttggtttcag
    12541 ggttgaagat cgcaacgttt gaaacatcga ttgcagcttc tttcttcact aaaccaccag
    12601 ctttgcctaa tgcaggaact ggtttttcgt gtttagtgat gatgttgata ccctcaacaa
    12661 aaactttacc atttggtaac actttagtta ccttaccacg tttgccctta tctttaccgg
    12721 taagaacaat tacttcatcg ttttgacgga tttttgcagc cattacattc tccttatagt
    12781 acttctggag ctaaagagat gattttcata aacttctcag aacgaagttc acgagtcacc
    12841 ggtccaaaaa tacgagtacc gattggttgc tcagtgttat tgtttaaaat tacacaagca
    12901 ttgccatcga aacgaataac tgcgccatct gggcgacgaa cacccttctt ggtgcgcaca
    12961 acaactgctt ttaacacatc accttttttc actttaccgc gtggaattgc ttcttttaca
    13021 gtaactttaa tgatatcgcc aatagcagcg taacgacggt gcgatccacc tagaaccttg
    13081 atacacatta cgctacgagc ccctgagtta tcagcaacat ccagcatagt ctgttcttgg
    13141 atcatattag tgctccgtta aaataaaaaa acaccctttc gggacgaacc tatttataca
    13201 aatataaata gggtgcaaaa tatactacaa aaaccactag tt
//
LOCUS       DANLXQ010000062        13174 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.062, whole genome shotgun sequence.
ACCESSION   DANLXQ010000062 DANLXQ010000000
VERSION     DANLXQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.062"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            74..979
                     /locus_tag="PXD94_001370"
     CDS             74..979
                     /locus_tag="PXD94_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL6286233.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQDAKNISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEVGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVEGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKEGK"
     gene            981..2222
                     /locus_tag="PXD94_001371"
     CDS             981..2222
                     /locus_tag="PXD94_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL6286234.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQQLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            2219..2851
                     /locus_tag="PXD94_001372"
     CDS             2219..2851
                     /locus_tag="PXD94_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL6286235.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNPACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETSKLYFILQGQKVRYLTDDEVNDLKNRGK"
     gene            2853..3629
                     /locus_tag="PXD94_001373"
     CDS             2853..3629
                     /locus_tag="PXD94_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL6286236.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCENV
                     HVMAAVVPEGEDREAYKQTFIKNVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIASVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            3682..5040
                     /locus_tag="PXD94_001374"
     CDS             3682..5040
                     /locus_tag="PXD94_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL6286237.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMSKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLLPIIFSVARRLGGNILVYSLPIGLAMLLVHVLVPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNNFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEVLESALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLSFILSALLRAAQGSATVATITTTTILAPAAVAAGYNDLQLALL
                     TAAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            5102..6442
                     /locus_tag="PXD94_001375"
     CDS             5102..6442
                     /locus_tag="PXD94_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL6286238.1"
                     /translation="MLKKTLLFTLLLSHSLYAEDMIQENKSANITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSVE
                     QKGLLKLTSGTTLGKTEWTQTAEPDKIKTPYLDDFVAKQVSLDQSGNKLNYRLYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYNSPEVIGWLLQQKRH"
     gene            6454..7143
                     /locus_tag="PXD94_001376"
     CDS             6454..7143
                     /locus_tag="PXD94_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL6286239.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
     gene            7300..9117
                     /locus_tag="PXD94_001377"
     CDS             7300..9117
                     /locus_tag="PXD94_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL6286240.1"
                     /translation="MIIKDTHWTSQQASPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            9120..9737
                     /locus_tag="PXD94_001378"
     CDS             9120..9737
                     /locus_tag="PXD94_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6286241.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(9821..10432)
                     /locus_tag="PXD94_001379"
     CDS             complement(9821..10432)
                     /locus_tag="PXD94_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL6286242.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            10627..11535
                     /gene="glyQ"
                     /locus_tag="PXD94_001380"
     CDS             10627..11535
                     /gene="glyQ"
                     /locus_tag="PXD94_001380"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL6286243.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            11615..11875
                     /locus_tag="PXD94_001381"
     CDS             11615..11875
                     /locus_tag="PXD94_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286244.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            11918..12952
                     /locus_tag="PXD94_001382"
     CDS             11918..12952
                     /locus_tag="PXD94_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL6286245.1"
                     /translation="MKTVIADNSAEFVNEISQLIQSSKQRMTVAVNAELTLLYWHIGK
                     RINDYILQGERAEYGQEVVKNLAQSLTEQFGKGWSKRHLNYTMQFATTFPNLEIVHAL
                     RAQLSWTHFKQIIHIDDPTKRGFYATMAAQERWSTRTLEDRIGSLLFERTAISKKPDE
                     TIIAELSLLREKGEYNQSMVLKDPYILDFLDLNDRYMERDLEDAILREIEQFLLELGA
                     GFTFVARQKRIQIDNDDFYIDLLFYNRKLKRLIAIELKTESFKHSHKSQMELYLAWLA
                     KYEQEADENPPLGMILCTGKKQEQVELLDLKNSDIHIAEYMTVLPSKAVLEQRLHLAV
                     ERAKERFLLK"
BASE COUNT         3915 a         2589 c         2969 g         3701 t
ORIGIN      
        1 atcacagatt ttatcttttc tattggttaa tataccgcca tcaagttcat taatttattt
       61 gagggaataa acaatgagtc aaaaatatag cgtagccgtc atcggcttag gagcaatggg
      121 tatgggagca gcaaaatctt gtatcgctgc cggattagac acatacggtg tggatcttaa
      181 cccgaattat ctggaaacct taaaagcagc aggtgctaag aaagtcggca caagtgcggt
      241 cgattttgcc gctgaattag atgcggtgtt gttgttggtg gttaatgcca accaagtgaa
      301 tgcggtgtta ttcggtgaaa acggcttagc ccaacatttg aaacaaggta cggtggtgat
      361 ggtatcttcc accatttctg cacaagatgc aaaaaatatt tcacagaagt taacagattt
      421 aggcttggtg atgttagatg ctccggtttc gggtggggca gcaaaagcag cacaaggtga
      481 aatgactgtg atggcatcag gttccagcga ggcattcgag aaattaaaac cggtattaga
      541 tgcggttgca ggcaaagtgt ataacattgg cgaagaagtc ggcttgggtg caacggtaaa
      601 aattatccat caattacttg ccggtgtgca tattgccgca ggtgcagaag caatggcatt
      661 ggcggctcgt gccggtattc cgcttgattt aatgtatgac gttgtaacca atgcggcagg
      721 caattcttgg atgtttgaaa accgaatgaa acacgttgtt gagggcgatt acaccccact
      781 ctctatggtg gatatttttg tgaaagattt gggattggtg aacgacaccg caaaatcttt
      841 gcgtttcccg cttcacttag ccagcaccgc ttattctatg tttaccgaag ccagcaacgc
      901 aggttacggc aaggaagatg atagtgcggt cattaaaatt ttcagtggta tcgaattacc
      961 taaaaaagag gggaaataag atgttaggca ttattgcaga cgatttcacc ggtgcgagtg
     1021 acatcgccag tttcttaacc gaaaacgggc taaaaacggt gcaaatgaat ggtgtgccga
     1081 aaactacact taacagtcaa gttgatgcga ttgtgattag cctgaaatct cgctctaacc
     1141 ctgtggaaga agcaatccaa caatcattac aagccttaga ttggctgaaa caaaatggtt
     1201 gctcgcaatt ctactttaaa tattgttcca cttttgacag cacggagaaa ggcaatatcg
     1261 ggccggtaac cgatgcccta atggcggcat tagataccga ttttaccgta gtcacaccgg
     1321 ctctgcctgt caatggcaga accattttca acggctattt gtttgtgggc gatgtgctac
     1381 tgaatgaatc tggtatgcgt aaccacccga ttaccccgat gaaagatgcc aacttggtgc
     1441 gtttaatgga tgcacaatct caaggcaaaa ccggcttggt tgcctatgct gatgtgattc
     1501 aaggggcaga gcgtgtgaaa gcccgttttg ctgaattgaa acagcaaggc tacaaatatg
     1561 cggtagtaga tgctgccgat aattcacaac ttgcggtgct tgccgaagcg gttgccgatt
     1621 ttcaattagt cacaggcggt tcaggcttag gggcttatat ggcggcttac cgttcaggcg
     1681 gcaaaaaagg cacgaatgcg tttgtaccaa gcaaaggcaa aaccgttgtg ctttcgggtt
     1741 cttgctcggt aatgaccaat acccaagtgg cggcttacca gcaaaaagcc ccaagttatt
     1801 tcttagaagc ggaaaaagcc attcaggatc cgaactatgt agaaacgctt tatcaatggg
     1861 tgattgcaaa cctagacggt gactttgccc caatggttta tgccaccgtt ccccctgaaa
     1921 aattgaaaga gattcaaacc caattcggcg gcgataaagc cagccacgcc attgaaaata
     1981 cctttgccaa attagccgaa aaattacaag ccgcaggcgt aactaacttt attaccgcag
     2041 gcggtgaaac ttcgagcatt gtggttcagc aacttggatt cagcggtttc cacatcggca
     2101 aacaaattgc accgggcgtg ccgtggttac aagcggtcga aaaaccgatt tttcttgcat
     2161 tgaaatcggg taacttcggt aaagaagatt tcttcagttt tgctcaggag atgtttgtat
     2221 gacagaacaa gaacaaaaga ttgaaatggt caatcttgcc cgctcgctgt ttgagcgggg
     2281 ctataccgtt ggcggtgcgg gcaacctctc cgtgcgttta gatgaaaatc gcattttagt
     2341 taccccaacc ggctcttcgc tcgggcgttt agaggcagac cgcctttccg tattggatat
     2401 gcagggtaat gttctttcag gagataaacc ttcaaaagaa tcggtattcc acttggcgat
     2461 gtatcagaaa aatccggctt gccaagcaat agtgcatctg cattccactt atttaaccgc
     2521 actttcttgc ttggaaaatt tggataccga caacgctatg cgtgctttca ccccttacta
     2581 tgtgatgcgg gtaggaaaat tgccggtcat tccttactac cgtccgggcg acatcaacat
     2641 tgcccgagag ctgggggaac gagccttaga cggcaaagcc tttttactgg caaatcacgg
     2701 ggtggtagtc acaggttcag acttgcgtga tgcggtggat aacacagaag agttggaaga
     2761 aacctccaag ctctatttta ttctacaagg tcaaaaagtc cgttatctca ccgatgacga
     2821 agtcaatgat ctgaaaaaca gaggaaaata aaatgcctaa atttgcagca aaccttacga
     2881 tgatgtttaa cgaagtgccg tttttagatc gttttgaagc ggctgccaac gctggattta
     2941 aatatgtcga gtatttatgg ccttatgatt atccggcagc agaattgaaa gccaaacttg
     3001 agcaatatgg cttaaaacag gtgttgttca ataccgcagc cggagatgtt gctcaaggtg
     3061 aatggggtgt ttctgccatt ccggggcgtg aagctgatag ccatcgtgat attgatttag
     3121 cgttggaata tgccctcgcc ttaggttgtg agaatgttca cgttatggca gcggtcgtgc
     3181 cagaaggcga agatcgtgaa gcctataaac aaacctttat caaaaacgtg caatatgccg
     3241 ccgataaatt caaaccgcac ggcattaacg tcttgcttga agccctaagc cctgaagtga
     3301 aaccgaatta tctgcttaaa agccagtttg atacgctaga aattgtggaa gcggtaaacc
     3361 gagacaatgt gtttgtacag cttgactact tccacgcaca gaatgtggac ggcaacttat
     3421 ctcgcctaac cgacaaactc aagggtaaat ttgcccacat tcaaattgcc tctgtgccag
     3481 atcgccacga accggacgaa ggggaaatta actaccaata tatcttcaac aaactcgatg
     3541 aaatcgggta tgaaggctat gtaggttgcg agtataaacc acgagcggaa accacctcag
     3601 gactaggttg gtttgagcag tataaataac cacgatgatt agcggataaa tacgtgaata
     3661 caacatttag aaggaaactt tatgtcaaat gaaatgttaa ttttgattgg attagcaagc
     3721 gtagttgctc ttctggtcat tatgattaaa ggtaaagtac acccattctt agcattgggg
     3781 ttagtgagta tcgcagttgc cttaactgca ggtattccaa tgagtaaagt gatcccaacc
     3841 ttgatttcag gtatgggaag tactttaggt agcgttgcac tcatagtagg tttgggagca
     3901 atgttaggga aaatcattga aaaatctcac ggtgcggatg tattagcaaa ttggttgtta
     3961 cagcgttttg gtgagaaaaa cgctcccttt gcattaggta tgacaggttt tatattcggt
     4021 attcctgttt tcgttgatgt tggttttatc gtattgctcc cgattatttt tagtgtagcg
     4081 agacgtttag ggggaaatat ccttgtttat tctttgccaa tcgggcttgc gatgttactt
     4141 gttcacgttt tagttccacc tcatccaggt attgtagcgg gtgctcaaaa cttaaacgca
     4201 gatattggct ttgtattggg aattggctta gtagcagcgc tacctgcata tttaatcggt
     4261 caaaaattta ttccgttctt tacaaaaaat aatttcgtaa aagtagtaac ttcaaccgat
     4321 cttttacagc accaaaaaga tttgattaaa gagaaaggca ttgaactacc ttcttttggt
     4381 aaagtgttag caatgatttc tttcccgcta atcctgatta tgttaggtac agtaacggca
     4441 acaaccttgc cgaaagaaca tatcgtgcgt gaagtattca gtatggtagg aagctctccg
     4501 tttgccttat ttgtggctgt ttgtctttct gcttatgtat taggttttag atacggttgg
     4561 actaagcata aattagaaga agtactggag tctgcgttag caccgattgc ggggattatt
     4621 ttaatcaccg gtgcaggcgg tatgttcggt aaagtgttag atgccagtgg cgtaggtaaa
     4681 gcattagcag atgtattagc caacacgggc ttaccgttat tggttctttc tttcatactt
     4741 tctgcattat tgcgtgcagc acaaggttct gcaacggtgg caactattac tactacaact
     4801 attctagctc ctgcagctgt agcagcaggt tataatgatc tgcaattagc acttcttact
     4861 gcagctattg gtgcaggttc tatgacatta tcccacgtga atgattcact cttctgggta
     4921 tggacgaaat tcttcaatgt tcctgtatcc gtaggcttaa gaacttggac gtttttaacg
     4981 actattttcg gtgtaatagc ctttgccgtt gttggcatta tttggatgtt attcgtttaa
     5041 tatcactctg ttactgctta aaatccacct cttatgaggt ggattttttt aggagaaacc
     5101 tatgctgaaa aaaacacttt tatttacgct attactttct cactccctct atgcagagga
     5161 tatgattcag gaaaataaat cagcgaatat tacgttgctt gccgaaatca ccgctcaagg
     5221 tcaaaaaatt aacggcgtgg cattggaata tcctgataat gtcttatcag gcagcgatct
     5281 tcgccaactg tatcaggtca aaacggcatt agatagtgcg gagaaagaaa accgttcggt
     5341 gctcaaagcc tatgtcaata atgagccaaa agtgaccgca cttccgcaag ccggtaaatt
     5401 tgtgattatt gaattggata cggcagataa aaatgcggca acttatagcg taaaagatgc
     5461 taataaagcg ccgatgaaat ttaaatccaa agatgaaaac ggcaacattg tagaaacaga
     5521 gaaagtacaa gcggtcaaaa ttccagagtt ttttgcaaat cgcctaatct attcggtgga
     5581 acaaaaaggc ttgcttaaat taaccagcgg cacaactcta ggcaagactg aatggacaca
     5641 aaccgctgaa cccgacaaaa tcaaaacccc ttatctcgat gattttgtcg caaaacaagt
     5701 gagcttagac cagtctggaa ataaactgaa ctaccgtctc tattcgcctt tgcaaaaaaa
     5761 tggtgaaaaa tacccgcttg tgattttcct acacggttca gggcaagtcg gttcggataa
     5821 ccttgctcac ctgctttcca gtaaaggggc gattgccacc ctgccttatg ccccaagttt
     5881 tgtacttgct ccgcaatacg accaagtatt cgacccattt gataatgccg ccaaagggca
     5941 aaaaggcggt attcactggc aaacggaaaa tcgtatcaat ctcgtgttaa aaatgattga
     6001 tgaaacccta aaagccaatc ctcaaattga taccacacgc atttatatca atggcttatc
     6061 tcgaggtgct gaagggggct taaatctgtt gttggctcgc ccgaatttct ttgccggagc
     6121 attattaatg agcggtcgtg aagcctacac caacgaatgg gtggacggca atgccaccaa
     6181 aacgaatttg gcggcaatta aacatatacc gatttggttt ttccatagta aagaggacaa
     6241 agtctcgccg gtaaaaggtt ctcgcattaa ctaccgcatt ctcaaagatg agttaaatgc
     6301 ccccaatgtg aaatataccg aattcacttt tgaacaagcc ggcgacaacg gcattgtcaa
     6361 taataaccct cataatactt gggacgctgt gtataacagc cctgaggtga ttgggtggtt
     6421 acttcagcaa aaacgccact aaggaggctc actgtgttta tgtatttgtc tcacccgtta
     6481 gatcctaaag atttagcttg gccgggagaa cctgttgttg aggtgactcg ctgcacagac
     6541 gtaggtgaag aaacgccatt tagcagtttt atcacgaaac taccgaatca ttgcggcaca
     6601 catatggacg ctcctcgcca ctttgtgaaa gacggtttaa gtatcaatga attgccgata
     6661 gagtatttct gccataaaga ggtcgcttta attgaaattc ccaaaggaga ggcagaaggg
     6721 atcaccaaag aagatttatt gccttatgtc gatattctct ccaaggtgtc ttttgccttt
     6781 ttacgaacgg ggtttgagaa atatagaacc gagaaccctg ctatttatca aaatgaatgc
     6841 ccctatattt caaccggtgc cgggcagtat ttatctgata ctttcccgaa tttgaaagga
     6901 gtaggcattg atttcttggc actagggtct ccttcttccc gagtgcctga tgcggaaaac
     6961 cctaaaaatt gccatcgagc gattttaggc tactataccg gcaggtttac cactgtgatt
     7021 gaagatatgc acttagcgga actccctaaa aatgccaaca ttaagcagtt ttttaatgca
     7081 ccgtggcgaa ttaaagggtt agattccagt caagtgactt gtattgtgga attacagcaa
     7141 taaaccacag atatattcat tttaggagag gctatttact atagtaagca taactcaatc
     7201 attaattgaa aataattatt atttgatttt aatcaagtca ttcataactt aattttgtta
     7261 taaatatgac cttgtttata gcactatttt gtttcgataa tgattataaa agatacacac
     7321 tggacatctc aacaagcctc tccaaatgct ttcccagaac gacaggcact tatgcctatt
     7381 tggggtggga aaaatattga acgcaatcaa tggcaatctt tatggctgga acaagcacca
     7441 aatgcgttat tacaggatgg tttagcttat ttccatattc cgttttgcgc tagtcattgt
     7501 attttttgtg gtttttatcg caatgcttgg aaggaaaatt atagccagat ttatactgat
     7561 cgtgtgatta gtgaacttcg atatgaatct tcactatggc gaggacaagg taagattcat
     7621 gccgtttatt ttggtggagg aacgccaaca gcgttacata cagaagattt agtacgattg
     7681 attcgagctt gttatcgtta tttgccactc gctgaagact gtgaatttac tattgagggt
     7741 agaattagcc attttgatat agataaggct aaggcttgta ttgaagcagg cgtgaaccgt
     7801 atttctattg gtattcagac ttttaactcc gaaattcgca aacgtttagg gcgcaaacat
     7861 agtggcgaag aggctcaaac ttatttaaaa gccttatcgg aattaaatgc tgtgattgtt
     7921 gctgatttaa tttttggctt gccaaaccaa acagatgata tctggcaaaa cgatattgaa
     7981 attgcgacta gtttaccgat ctcaggctta gatatttacg cctttaataa ttatcctttc
     8041 ctgcctatta atcgcttaat tgaacatgga aaattacctg cgcccgcagg gtttgacacc
     8101 caaagcttac actatgctta tgcagtagag aagttaaaag cacaaggttg gcagcaagtg
     8161 agcaataacc actttgcttt tccaaatcga ggtgaacgca atcgctataa tactttggtt
     8221 aaatcgaata tgccttgttt ggcatttggc tccggtgcgg ggggaaattt tggtgggctg
     8281 agttaccaag tacaatctga tttaaagacc tacttagatt cgcctcttga tcagaaaagc
     8341 ctttcttttt tgagtaaaca tggtgaacat aaagcactgc ttggtgtgat acaacatagc
     8401 atggaattgg gttacttaga tacaacgcct tttgaacaaa atgcgaaagc tgaaaaatta
     8461 ttgcaacaat gggaagcgct tgatttactg aagcgtgaaa aaaatcaagt atatctcaat
     8521 accagtggtc gttactggtc accaacatta attcgtaaac taatgttaac acttcctcaa
     8581 tcaagtaagg ataacaacat gataacgcca ttaactaaag aattagaaaa taaattgcgg
     8641 acaaatttaa aagaaaaccc agggcaaatt ttagaaatgc tggcaggaat gaatcaatgt
     8701 agcttggaat cggtaattaa ctgtctgccc aaagaaatgg taatgcaaac aggtggtgag
     8761 cgttttgtgg aaattatgca ggcaattgct ggttggaatg aagctgtgac ttttattatt
     8821 catacgcctg atgcgattgc agaggtaact gctaaattgc cacaaggtaa aattgggcga
     8881 ggtttttata attttgaaca tggtgtagat ggaggaattc acggtcattt acgctatgaa
     8941 aattgtgctg ctatttactt acttgatcgc ccatttatgg gaaaaagaac ggtggcgtta
     9001 acttttatca atcaaagcgg tgaggcgatg tttaagattt ttgttgggcg tgatgaacaa
     9061 ggtcaattac gagaaaatca gattcaagcc atgcggaaat taattggagg atgctagtca
     9121 tgattttggt ttgtggggca aatgggttgt taggacgagc aatctgtgac aaattaccta
     9181 gagagaaaac gatagccgtc ttacgaaaag atgctatgga tacgtttttc agcgaacgaa
     9241 atatagcagt agaaatagca gatttattag atttggaatc ctgcaacgcc ttgttacaaa
     9301 aaacacaacc ggaaatcttg attacggtag taggaggaaa aaatgaacaa ggtattcgta
     9361 gcgacaaaac agcaaatatt aatttgataa aagcagttga aaaatattca ccaactacga
     9421 aagtagtttt agttactagt gttgggtgtg cagaacaatg ggatttaatt agcccattag
     9481 cacagcaagc tttaggtgaa gcactaaaag ccaagacgga agctgaagct tatttacagc
     9541 aaacagcatt aaattggctg attattcgac ccggtgggtt aaataatgat gaagtaaatg
     9601 atgagtatca gattgtgcgt gatttaccta atgaacggaa aatgtatgta agccgtaaat
     9661 ctgtcgcaac aggcatagtg cgtttactgg atagtacgga aaatcatcaa atcttctcta
     9721 ttattaatag gggatagtat atttcaacga aggcaagcgg gcatatttta tggttatttt
     9781 gcaactataa aacagaccgc ttgtgagatc tttttctatt ttattgagaa ctggatgccg
     9841 cattcatctg caagtagcga agcacctttt tctgggtagc ttcgtccata tcggtaaaac
     9901 cgaccattcc gttaaatact gcaatccatt cgtttgcatc atgcatttta ggatcacgtt
     9961 gtttatggca cacgctgcat tctgttttat aggtattttt ggtgttcgcc caaaagttag
    10021 tgagatcttg ggtaaattcg gcattttcca cccacacgtt cattttcact ttttgccata
    10081 ccaagccggt gttcggatct tccttgctct ctacaatctc taacagtggg ttgttgctga
    10141 taaaagattt ctcaaatacc gcatcggtaa tatttttact gaaagaatga taccaaatcc
    10201 gcccgaaacc tttgcttttt cgccataaat tcagctcaat ttcagcggct tttgcggttg
    10261 tttgcaccaa tttaatttct gctccggctt ctagctgccc gattttctgt gtcaaatttt
    10321 catcggcaaa taaagggatt gtttccgtgg tgtaactgtt tgaaccggca aaacaaaatg
    10381 ttggcatgat gaatgccaag aaacagaaag cgattttcga taatgatttc atcataaacc
    10441 ccacaaatta gatgagaaat taggttatat gttacacaaa ttgagcaaaa aagcgttgat
    10501 ttacatcata aaactgattt tatgtcgatt tcttaatttt ttgcaaaaaa atcccaaaaa
    10561 atgactgctt acaagctata ataaccagta tttttcgacc atttttcaaa ttcaagagaa
    10621 aataaaatga gtacaaaatt caacgtaaaa acctttcaag gaatgatttt agccctgcaa
    10681 gactactggg caagcaaagg ttgcaccatt gtgcagccgt tcgatttaga agtgggggca
    10741 ggcacctcgc acccaatgac ctgcctgcgt gcgcttggcc ctgagccaat ggcgtttgcc
    10801 tatgtgcaac catctcgccg cccgacagac ggtcgctacg gcgaaaatcc gaaccgttta
    10861 cagcactact accagttcca agtggtgatt aaaccttcgc cggataacat tcaagaactt
    10921 tacctcgaca gcctgaaaat gctcggtttc gacccaaccc aaaacgacat tcgttttgtg
    10981 gaagacaact gggaaaaccc aacgcttggg gcgtggggct taggttggga agtgtggctg
    11041 aacggaatgg aagtgaccca attcacctac ttccagcaag tcggcggctt ggaatgtaaa
    11101 ccggtgacag gcgaaatcac ctacggctta gagcgtttgg cgatgtacat tcaaggcgtg
    11161 gacagcgtgt acgacctcgt gtggtctgac ggcgaactcg gtaaaaccac ctacggcgac
    11221 gtattccacc aaaacgaagt ggagcaatcc acctacaact ttgaatacgc tgatgtggac
    11281 ttcctgttcc aatgcttcga gcagtacgaa aaagaagccc aatacctgtt aggcctagaa
    11341 aaaccattgc cattgccggc atacgaacgc attttaaaag ccgctcacag ctttaactta
    11401 ttagatgctc gtaaagcgat ctcggtaact gaacgccaac gctacattct acgcattcgt
    11461 gccttaacca aaggcgttgc tgaagcgtac tatgccagcc gtgaagcgtt ggggttcccg
    11521 gggtgtaaaa aataatcgta gggacgccac gcttggcgtc cgtcaataaa ttacaaaacg
    11581 gacacgaagc atcgtgtccc tacaaggatt tacgatgaaa ccgattttat acttcgccga
    11641 ttggtgtccg gataccgccc catttgtggc agaactagac cgcttgggca ttgaatatga
    11701 gccgtgcgac atcaccaaag gaggctcaac acttaaacca tttttgaaac tgcgagacac
    11761 gcattctgcc tttgaccacg ccaaagccaa cggctatatt ggcattccgg cattgctgat
    11821 cgaaggcgaa aaagtcgtgc tggatttggc tgaattagag ggtattttcg gctaacaagc
    11881 ggttgaattt ggcggaaatg ttgcaaagga gccaataatg aagacagtaa tagcagataa
    11941 ttcggctgaa tttgtgaatg aaatttcaca acttattcaa tcgagtaagc aacgaatgac
    12001 cgttgcggtt aatgcagaat taaccttgct ttattggcat atcggaaaac ggatcaatga
    12061 ctatattttg cagggtgaac gtgcggaata tgggcaagaa gtggtgaaaa atcttgctca
    12121 aagtctgacg gagcaatttg gtaaaggttg gagtaaacgc catttaaatt atacaatgca
    12181 atttgctaca actttcccta atttggaaat tgtgcacgca ctgcgtgcac aattaagctg
    12241 gacacatttt aagcagatta ttcatattga tgatccaaca aagcgagggt tctatgcaac
    12301 aatggctgca caggagcgtt ggagtacaag gacattagaa gatcgcattg gctcattatt
    12361 gtttgaacgc acggcaattt ccaaaaagcc cgatgaaacc attattgctg aattgagttt
    12421 gctacgtgaa aaaggcgaat ataatcagtc aatggtgttg aaagatccct atattttgga
    12481 ttttctcgac cttaacgacc gttatatgga acgtgatctc gaagatgcga ttctacgaga
    12541 gatcgaacag tttttactgg aacttggggc gggttttacc tttgtcgccc gccaaaaaag
    12601 gattcagatt gataatgatg atttctatat cgatctgctg ttttataacc gtaaactcaa
    12661 acgcctgatt gcgattgaac taaaaaccga aagcttcaaa cattcccaca aaagtcaaat
    12721 ggaactctat ttggcgtggc tggcgaaata cgagcaagaa gcagacgaaa acccaccgct
    12781 tggaatgatt ctctgcaccg gtaaaaagca ggagcaagtg gaattgttgg atttgaaaaa
    12841 ttccgatatt catattgcgg aatatatgac cgtgttgccg agtaaagcgg tattggaaca
    12901 acggttgcat ttggcggtgg aaagggcgaa agagcggttt ttactaaaat agcaagcggt
    12961 taaaatcagc ggaaatgttg caaaatgaaa gtatgatgga aacgatttgg ttgcatattc
    13021 cgttggtggg tggttagaga ttttcaaatc tcccccctcc ccctctttgg ctaaagaggg
    13081 ggcgatcatt tagtgagtaa ttttaattca tctgactatt tacaatctta atgaagaaag
    13141 atgaattata aatttacgaa gatcaaattc ccct
//
LOCUS       DANLXQ010000063        13009 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.063, whole genome shotgun sequence.
ACCESSION   DANLXQ010000063 DANLXQ010000000
VERSION     DANLXQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.063"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            57..1070
                     /gene="cas1c"
                     /locus_tag="PXD94_001383"
     CDS             57..1070
                     /gene="cas1c"
                     /locus_tag="PXD94_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL6286246.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQDRKKVAQLPIHSIGHI
                     FCFGNVLVSPFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNP
                     VPVARNIIAAKIQASKRVLQRQIRNHGENADLQSTITALNYSLQQLKTADNLDLIRGI
                     EGDAAARYFGVFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSIVGKDISGALQGV
                     GLDPQIGFLHADRSGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAV
                     TLKPEARKLLFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPF
                     LMR"
     gene            1137..1430
                     /gene="cas2"
                     /locus_tag="PXD94_001384"
     CDS             1137..1430
                     /gene="cas2"
                     /locus_tag="PXD94_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL6286247.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     repeat_region   1622..2123
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1622..1641
                     /rpt_unit_seq="cagccacctacgcgtggctg"
     gene            2373..3848
                     /gene="rng"
                     /locus_tag="PXD94_001385"
     CDS             2373..3848
                     /gene="rng"
                     /locus_tag="PXD94_001385"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL6286248.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     gene            4002..5504
                     /gene="lysS"
                     /locus_tag="PXD94_001386"
     CDS             4002..5504
                     /gene="lysS"
                     /locus_tag="PXD94_001386"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL6286249.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLVMIFANAPSIRDVILFPAMRQK"
     gene            complement(5679..6734)
                     /locus_tag="PXD94_001387"
     CDS             complement(5679..6734)
                     /locus_tag="PXD94_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6286250.1"
                     /translation="MGRPRKQQNRYLPENLRPKTVTRKSGKTVIYWLYRTHGKTEISL
                     GTNRNEAFIKAAQLNIERESQTARITFGLVAKRYRDEVIPQKKASTAQTDLCKIKNLL
                     LFFNDAPLEEITPQHIREYMTWRKDTPGSANNEFSMFSHIWQYAREWGYTNLPSPNIG
                     MKRYPIRKREIYVENNIFQMVYEVADQDMRDLMDLAYITGQRPVDLVNIRKEHIFDGY
                     LHIVQQKTQAKLRIEIVGKLADIIQDRLEKATGTHLFSTKKGEKLTARKLGKRFTYIR
                     EQAVKNNPEYANEIMAFQFRDLRAKSGTDKALSLGEEAARQQLGHTSVQMTKTYIRKA
                     PIVTPIKESVPSSKHQS"
     gene            complement(6694..6969)
                     /locus_tag="PXD94_001388"
     CDS             complement(6694..6969)
                     /locus_tag="PXD94_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL6286251.1"
                     /translation="MEQPTEFLTKDELADLTGYRQKKRQIEWLRERHYIIQDENKYKP
                     LVLYKDVYGHPRQQYPEAQSTGKWKSPAYLMGVKNGKTTQTTESLLT"
     gene            complement(7059..7565)
                     /locus_tag="PXD94_001389"
     CDS             complement(7059..7565)
                     /locus_tag="PXD94_001389"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL6286252.1"
                     /translation="MRILPRNENYAATENGEIFSFLSNKILKPRPLKNGYLRVQLVDE
                     NGVKDYLIHRLVCETFHGYPDLHVNHKDRDKTNNRPENLEWCTRSENMLHCSRLGGFS
                     KQSERMKKMNIESCSVPVAAITLDGKLFKKYTSMTSAGKDGFSHSKISLCIQGKRKTH
                     KGYIWQKL"
     gene            complement(7543..8139)
                     /locus_tag="PXD94_001390"
     CDS             complement(7543..8139)
                     /locus_tag="PXD94_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL6286253.1"
                     /translation="MPLSITANLTKAVQLKHQGEALSGGSETLVVHGTQDPIISQNKA
                     HCLGRNNGQENVLFEVKGAEAVRISEIQDKTPTLKARMGTGGNNIPCIALAGNTIGRQ
                     PQNDGNGNVFDESGVSYMLTRTDVHAVSAGTTIRKLTPTECERLQGFPDGWTKITYRG
                     KSAEECPDSPRYKAIGNSMCTNVMKWIGERLSAYLTSQ"
     gene            complement(8060..9379)
                     /locus_tag="PXD94_001391"
     CDS             complement(8060..9379)
                     /locus_tag="PXD94_001391"
                     /inference="COORDINATES: protein motif:HMM:NF024795.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HDL6286254.1"
                     /translation="MNNIIKFTYGSICSGIEAASVAWHDIGTPLWFSEIEPFPCAVLA
                     HRFPDVPNLGDMTALPEKILNRKIPAPDVLVGGTPCFTAGHMVLTDKGYMPIETLSVG
                     DLVVTHKGQLKPILRVGSEIKPVGKLTAVGLPESIVCTPEHPFYAQKWQTINTKRNGK
                     YHRKTIISKPEWIEAHKLEGYQWVSLTDFSVMPYGGFHSKLTDDEVLLIAGYYLGDGW
                     IRRWKGKNKKAVVISVNKEKYRKFSLSFAKLPHHITTENNSVIKITICDTELADFLYS
                     QFGEKAGGKTIPAWCLSHSFRSKIFEGYMITDGSFRNGVYTANSISKSLAYGIAALSQ
                     TLGYISSVSKVTVAPTKEIQGRIVNQNDYYQMRAFLQSTSRKSRLDENRLLRSVQSFE
                     ITGNEVVYNIEVADDNSYILNNAVVHNCQSDKGGTVKASGGGFEWRE"
     gene            complement(9376..10281)
                     /locus_tag="PXD94_001392"
     CDS             complement(9376..10281)
                     /locus_tag="PXD94_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDL6286255.1"
                     /translation="MQFQEHNNRKKADKFAEYITGEALRRYVAEKVKQYARNNVSVFD
                     GAAGSGQLEQFVRPTSFTAVEVQSESCDVLRHNFPTAQIYNQSFFLYPNGEPCDCVIM
                     NPPFSLKFKDLSQAEQAQIQSEFPWKKSGVVDDIFVLKGLANSHQWGFFILFPGVGYR
                     QTEKRFREEVGNQLVELNRIQNAFEDTPIEVLFLVVDKTKTTDDCSRELIDCAGGQIQ
                     QICADKWKIRPDHWESIQPPPPPQEEIKPLELEAFAREQAIERIVGEVRLSKLIAEFF
                     EPELRRTFNAFIDDICVAVQAEKIV"
     gene            complement(10515..11192)
                     /locus_tag="PXD94_001393"
     CDS             complement(10515..11192)
                     /locus_tag="PXD94_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL6286256.1"
                     /translation="MSNQVQFPVFNFNSSAVRVIIDPNQEPWFCGADVCRILGYVNES
                     LTLQKHCKENGVSKRYLTDKMQRQQKAIFINEPNLYRLIIKSRKPEAEKFEAWVFEEV
                     LPQIRKTGKYALQNNQQNLPLAPPPKKYSFDFTEDELQSLIWAWFAFVRGIHTFRYIY
                     PMFQKLGSNIAPEIYGQGFEYSHTAQSAHKILERITKEFDYDPMTNWRVLKHVRGFDP
                     AFKKPTF"
     gene            complement(11440..12279)
                     /locus_tag="PXD94_001394"
     CDS             complement(11440..12279)
                     /locus_tag="PXD94_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDL6286257.1"
                     /translation="MANSVMIVQGVEVKVTARDGEDYISLTDMCKAFGDSDQLIKNWL
                     QNKNTIEFLQVWEELNNPNFNLVELHQIKNNIGLNRFVMSVKKWTATGAIGLVAKAGR
                     YGSGTYAHKDIALEFGSWLSPEFKLYLIKEFQRLKQQEANESKLEWSVKRILSKANYR
                     IHTDAIKAHIIPALLNTKQHTFVYSSEADILNQALFGQTAKQWKDRNPNLKGNMRDHA
                     TVEQLTVLAALESQNALLIEQGYSQEERLAMLNRLAIQQMSSLLQTRAIEELKEKPLL
                     IEE"
     gene            12509..12886
                     /locus_tag="PXD94_001395"
     CDS             12509..12886
                     /locus_tag="PXD94_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286258.1"
                     /translation="MFSLLRDALTFAAMFTGILFPAIIHKYDLSLGTQIQLLSFVPAF
                     FVLCHFLIKRKYQRDFDRLSYLSAVVCVAMCAGVGAFSQSEMIELGFKVERVLDYVTI
                     KFLLYFWSLLLLPVVLRKLFGKD"
BASE COUNT         3578 a         2491 c         2820 g         4120 t
ORIGIN      
        1 gaaaaatata tttaaataat tatgatgtga gattaaagtt aaagggattt tcaaccatgc
       61 gcaaactaca aaacaccctc tacattacca cccaaggtag ctatttgcac aaagagcgag
      121 aaaccttggt ggtggagcaa gatcggaaga aagtggcaca gttgccgatt cattccattg
      181 ggcatatttt ttgttttggc aatgtgttag tgtcgccgtt tttaatggga ttttgtggcg
      241 aaaataacgt aaatttagcg ttttttaccg aaaatgggcg ttatcttggg cggttgcagg
      301 ggcggcaaag tggtaatgta ttgttacgcc gagcccagta taaaaagtcg gagaccaacc
      361 ctgttcctgt ggctcgcaac atcattgcag ccaaaattca agcatctaag cgggtgttac
      421 aacggcaaat tcgtaatcac ggcgaaaatg ctgaccttca atcgacaatt accgcattaa
      481 attacagcct gcaacagttg aaaacagcag acaatttgga tttgattcgt ggcattgagg
      541 gcgatgctgc ggctcgttat tttggggtgt tttctcatct tatcaaagaa aatagcggtt
      601 tccactttga cggacgtaat cgccgcccac ctcgtgacgg tgtgaatgcg ttgctttcat
      661 ttctttatag cattgtaggg aaagatatta gcggggcatt gcaaggcgtg gggcttgatc
      721 cccagattgg ctttttgcat gcagatcgtt cggggcgaga tagcttagct caagatattt
      781 tggaggaatt tcgagcgtgg tgggtggaca gaatggtgct gtcgctgatc aatcgagggc
      841 aggttaagcc tgatgatttt gtgacggaag ccagtggtgc ggtaacgctc aagcctgaag
      901 ccagaaaatt actatttcaa accttgcaag cgaaaaaaca agaaaaaatc atccacccat
      961 ttttagagga agaagtggaa atcggcttgt taccttatat ccaagcgatg ttattggctc
     1021 gtcatttgcg tggcgatttg gcagaatatc cgccgttttt aatgcgttaa aatctgcaaa
     1081 attttgttga aatttgaccg cttgtttatg ttttattcat tgaggaatta gcaactatgc
     1141 tgatgttgat tacctacgac atttcatttg atgaccccga agggcaggct cgcctacgcc
     1201 gtattgctaa gcattgtttg gattatggtg tgcgggcgca atattctgtg tttgagtgtg
     1261 atgttactcc tgctcagtgg gtaacattga aaaataaatt attggcaact tacaacccag
     1321 actgtgatag tttgcgcttt taccatttag gcagtaaatg gaggaataaa gttgagcacc
     1381 atggcgcaaa accttcagtg gatattttta aagatacgct gattttataa atcgctagcc
     1441 tgtagttctc ataaaaatgt agcaaggtta gcgaatctat attgttcttt aataatcaag
     1501 gaattagaaa aaataaggca caatatattg gcgatatgct atactttatc cactctctac
     1561 aaaacgttag cgaataaagg gctaaaaagc ctgatatttc aatacgttat cttataggga
     1621 ccagccacct acgcgtggct gcgaattgaa accaccgctg ctacttcggt tatcggagta
     1681 gtttgcaccc agccacctac gcgtggctgc gaattgaaac gtggcacagt aacacgagca
     1741 gatgctgata aacgcccagc cacctacgcg tggctgtgaa ttgaaacttt accgtttacc
     1801 gattggattt taatgctaga ggcagccacc tacgcgtggc tgtgaattga aaccctaaat
     1861 tgccgtaaaa atctgccatt agctacgcgc agccacctac gcgtggctgt gaattgaaac
     1921 ctcataacga atccaatcct gttcggttaa tccccgcagc cacctacgcg tggctgtgaa
     1981 ttgaaacagg ggcgtgtacc gcaatcaatt ttaattataa ccaagcagcc acctacgcgt
     2041 ggctgtgaat tgaaacctcg tcttgaattg gaaaatcaag ggcgacaata ggcagccacc
     2101 ttcgcgtggc tgtgtattga aacggtgtac ccacaaatcc tatgttttcg ccattggctg
     2161 ctaccttcgc atcaatatat attgaaatat caatggcaaa tttgtatgtt gttaattaga
     2221 ctgattgcat ctgctatgta tcatagtaat gacgaaaata ttctattttg caaaaaataa
     2281 tcagaaatta cccgcttgtt cgaactgagt ttttgttttc taagatgtga aatgatacac
     2341 tttagatagt tttaaaatat agagacagta agatgagtag tacggaatta ttagtgaatg
     2401 ttaccccaag cgaaacaagg gtagcgttgg tagaaaacgg aatattgaaa gaattacata
     2461 ttgagcggga agctaaacgc gggattgttg gtaatattta taagggaagg gtgacaaggg
     2521 tattaccagg tatgcaatct gcttttgtgg atatcggctt ggagaaggct gcttttttgc
     2581 acgcttctga catcgtttcg catacggaat gtgttgatga aaatgaaaag aagcaatttc
     2641 tggttaaaga tatttctgaa ttggtgcgtg aagggcaaga tatagttgta caagtagtga
     2701 aagatccaat tggtactaaa ggagctaggc tcactactga tattacgtta ccatctcgtt
     2761 atttagtgtt tatgccagaa aatagccatg tgggagtatc tcagcggatt gaaagtgaag
     2821 aagagcgtgc tcgtttaaaa agcttggttg agccttattg tgatgaactt gggggcttta
     2881 ttgtacgtac cgctgcagaa gaggcaactg aagaaagttt aaagcaagat gttgaatttt
     2941 taaaacggtt gtggcgtaaa gtattagaac gaaaagggaa atattccgca aaatcaatgc
     3001 tttatggaga gcttgcgctt gctcaaaggg tactgcgtga ttttattggt acacatatca
     3061 attcggttag aatcgattca aagctaactt atgagcaagt gcgagaattt ttagctgagt
     3121 ttatgccaga attaacggat tgtgtcaccc tctataccgg tcagcaaact ttatttgatg
     3181 cctatggtgt agaaaaggca atccaaaagg cattggataa acgggttgat ttaaaatcgg
     3241 ggggctattt aattattgag caaactgagg cgatgacgac tattgatatt aatactggtg
     3301 cttttgtcgg acatcgtaac cttgcacata cgattttcaa cacaaatatt gaagcaatac
     3361 aggcaattgc acaccagcta cagttgcgta atttaggtgg tattatcatt attgatttta
     3421 ttgatatgca agaggaagaa catcgtgagc gcgttttaca atcgttacaa gaggcattaa
     3481 aaggcgatcg tgttaaaacc aatgtgaatg gctttactca gcttggcttg gtggagatga
     3541 ccagaaaacg tacaagggag agtctagagc gaattctttg ttgcgattgc caagcttgcc
     3601 aagggcgtgg cacagtgaaa actgtagaga cggtttgcta tgaaattatg cgtgaaattg
     3661 tgcgtgtaca tcatttgttt aagacagaaa atattcacgt ttatgcagct aaagatgttg
     3721 ctgaatattt aattaacgag gaaagccacg cattattggc tgagcttcag gcgtttatcg
     3781 gtaaaaaaat tgagattaaa cttgagccat attatcatca agagcagttt gatgtggtcg
     3841 tgatgtaatc tattacttgg ttgaggctaa atagttagcc tcaatatcgt tagttacaaa
     3901 aaatttagaa aatctgaccg cttgtttcct atccaaattc ctcaaaataa cgtataatcg
     3961 tccagtttta tttatccttt tttgaaatct aaggtaattc aatgtctgaa gtagaaaatc
     4021 aagaattaga tctaaacggt gaaatgttag cccgccgtga aaaattaaac aaaatccgtg
     4081 agcagggtaa cgcatttcca aatacattcc gtcgtgatgc gttagccaaa gatctgcacg
     4141 ctcaatatga tgcagtggaa ggtgaaacct taaaagaaca gaatattcaa gtgaaagttg
     4201 ccggtcgtat tatgttaaaa cgagtaatgg gtaaggcatc tttctttacc attcaagatg
     4261 tgagcggtgc gattcagctt tatgtagctc gtgataactt aagtgacagc gtttacgaag
     4321 aaaaagtcag cctttgggat ttaggcgaca ttgtgggcgt cgaaggtgcg ttatttaaaa
     4381 ccaaaaccgg tgaattaacg gttcgttgct ctgaagttca acttttaacc aagtctttac
     4441 gcccgttgcc ggataaacat cacggtttaa cggatttaga aactcgttat cgtcagcgtt
     4501 atttagactt gatttctaat gaagaatctc gccgtacttt tatgatccgc tcgcaagtag
     4561 tttcaggcat tcgcaaattc ttcttagaaa gagactttat tgaagttgaa acgccaatgt
     4621 tgcaagtgat cccgggcggt gcggcagcga aaccgtttat cactcatcat aatgcgttag
     4681 atgtggatat gtatctgcgt attgctccag agctttactt aaaacgttta gttgtgggtg
     4741 gttttgagcg agtatttgag cttaaccgta acttccgtaa cgaaggggtt tcagtacgcc
     4801 acaatcctga atttacaatg attgagtact accaagccta tgcggattat cacgatttaa
     4861 tggataatac cgaagaactg ttacgccaat tagccttaga tattcttggc acaactgacg
     4921 taccttatgg tgattatgta ttcgatttcg gtaaaccgtt tgagcgtatt acgatgcacg
     4981 atgcgattgt gaaatatggt aacggcattc aacgtgaaga tttagacagc ttcgagaaaa
     5041 gtgtggaaat tgcgaaaggc ttaggcattg aaattcaaaa atcttggggt ttaggctcgg
     5101 tagtcaatgc gatttttgaa gaagtggcag aacatcaatt aattcagcca actttcttga
     5161 tggctcaccc ggcagaaatt tcaccattag ctcgccgtaa tgatgaaaac ccagaggtaa
     5221 ctgatcgttt tgagttattt atcggtggtc gtgaaattgg taatggtttc tcagaattaa
     5281 acgatgcaga agaccaagca gagcgttttg atgctcaagt tgcggcgaaa gatgccggtg
     5341 atgatgaagc aatgtttaaa gatgaagact ttatcgtggc attggaacac ggtctgccgc
     5401 caacagcggg tgaaggttta ggaattgacc gcttagtaat gattttcgct aacgccccat
     5461 caatccgtga tgtaatttta ttcccagcga tgcgtcagaa gtaatcagaa atagaacaaa
     5521 agcacacttc ggtgtgcttt tttgtattac aagcggtcaa atttttaata aatattatca
     5581 gtaataacga gtaacaaaaa ttacttatct ttcaatctat tataagtgat ttttatgtaa
     5641 acgttccgaa aaaatgcaaa atgttccgaa actgtctttt aagactggtg ttttgaggag
     5701 ggaacacttt ctttgatagg ggttactatt ggggcttttc gaatgtatgt tttggtcatt
     5761 tgaacgctag tatgacctag ttgttggcga gcggcttctt cacctagaga aagggcttta
     5821 tctgttcctg atttagctct tagatcccga aattggaatg ccattatttc atttgcgtat
     5881 tccgggttat tcttgaccgc ttgttcccga atataagtga accttttacc tagttttcta
     5941 gcggttagct tttccccttt cttagtgctg aataagtgtg ttccagtagc tttctccagc
     6001 ctatcttgga taatatcagc cagttttccc actatctcaa tacgtagttt ggcttgtgtt
     6061 ttttgctgaa caatatggag ataaccatca aaaatgtgtt ctttccggat attgaccaaa
     6121 tcgacaggac gttgtccggt gatataggcg agatccatta agtctctcat atcctgatct
     6181 gccacttcat ataccatctg aaatatattg ttctcaacgt aaatttcacg ctttctgata
     6241 gggtagcgtt tcattccgat attagggctt ggtaagtttg tataacccca ctctcgagcg
     6301 tattgccaaa tatgactgaa cattgaaaat tcgttattag cagatcccgg tgtgtctttt
     6361 cgccaagtca tatactctcg gatatgttga ggggtgattt cttcgagagg agcgtcatta
     6421 aagaatagca gtagattttt tatcttgcac aaatctgttt gtgctgtgct cgcttttttc
     6481 tgaggtataa cttcatctcg ataacgtttg gctacaagcc cgaaggtaat tctggcagtt
     6541 tggctttctc tttctatatt gagttgagca gccttaataa aagcctcatt tctatttgtg
     6601 cctagagaga tttccgtttt tccgtgcgtg cgatatagcc aataaatgac agttttgcct
     6661 gattttcgtg taactgtttt tggacgtaaa ttctcaggta agtagcgatt ctgttgtttg
     6721 cgtggtcttc ccatttttca ctcccattaa ataagcaggt gatttccatt tcccagtgga
     6781 ttgagcttct ggatattgtt ggcgggggtg cccatagaca tctttgtata gcactaatgg
     6841 cttatacttg ttttcatctt gaattatata gtgacgctcc ctcaaccatt cgatttgtct
     6901 tttcttttga cgatagcctg ttaaatcagc aagttcgtct ttcgttaaaa attcagtagg
     6961 ttgttccata ttctctccaa ataaccccct catttagagg gattgagtta aataatcgtt
     7021 aagcctttgt cctatccacc gcatacaagg gacggccatt ataacttttg ccatatgtaa
     7081 cccttgtgcg ttttgcgttt accctgaatg caaaggctga tttttgaatg tgagaaacca
     7141 tctttacctg cacttgtcat tgaggtgtat tttttgaata atttcccgtc cagagttatt
     7201 gcggcaacag gaactgagca actctcaata ttcatttttt tcatcctctc ggactgcttg
     7261 ctaaaacctc caagtcttga gcaatgcaac atattttctg agcgtgtaca ccactctaaa
     7321 ttttcaggtc ggttatttgt tttgtctctg tccttatgat ttacatgtag atctggataa
     7381 ccgtgaaaag tttcgcatac cagtctatgg attaaatagt cttttactcc attctcatct
     7441 acaagctgaa ctcttaaata accattcttt aaaggtctag gttttaagat tttgttgctc
     7501 aagaaactaa aaatttctcc attctcggta gctgcatagt tttcattgcg aggtaagata
     7561 cgcacttaat ctctctccta tccatttcat tacatttgta cacatcgaat ttcctatcgc
     7621 cttatatcgt gggctatcag ggcattcttc ggcagatttg ccacggtatg tgattttcgt
     7681 ccagccgtct ggaaagcctt gtagtctctc gcattcggtg ggagtaagtt ttcgaatagt
     7741 tgttccagca ctgacagcgt gaacatcagt tcgggtaagc atgtaactta caccactctc
     7801 atcaaatacg ttgccgtttc cgtcgttttg tggctgtctg ccgatagtat taccggcaag
     7861 ggcaatacaa gggatattat ttccacccgt ccccattctt gctttcagtg ttggcgtttt
     7921 gtcctgaatt tcgctaattc gtacagcttc agcacctttt acttcaaata agacgttctc
     7981 ttgcccgttg tttcgaccta aacaatgagc tttgttttgg ctgataattg gatcttgcgt
     8041 accgtgaaca actagcgtct cactcccgcc actcaaagcc tccccctgat gctttaactg
     8101 taccgccttt gtcagattgg cagttatgga caacggcatt atttaagatg tagctgttgt
     8161 catctgccac ctcaatgtta taaacaactt catttccagt tatctcaaag gattgcacgc
     8221 ttctcaataa tctattttca tcaaggcgac tttttcttga tgtcgattgg aggaacgctc
     8281 gcatttgata atagtcattc tgattgacaa ttcttccttg gatttctttt gtcggagcaa
     8341 cagttacttt ggaaacagat gagatatatc ctaaggtttg ggataatgca gcaatgccgt
     8401 aagcaaggga tttcgaaatg ctatttgcgg tataaactcc gtttctaaaa gagccatcgg
     8461 taatcatata cccttcaaaa attttacttc tgaaagaatg agataaacac caagccggta
     8521 tagtttttcc gccggctttc tcaccaaatt gcgaataaag aaaatcagca agttcagtgt
     8581 cacaaatagt gattttaatt accgaattat tttcggtagt aatatgatga ggaagtttgg
     8641 caaaagaaag agaaaacttc cgatatttct ctttatttac agaaataaca acagcttttt
     8701 tatttttgcc tttccatctt cttatccagc catcaccaag ataataaccg gcaattagaa
     8761 ggacttcgtc atcagtcagt ttgctatgaa aaccaccata gggcataact gaaaaatcag
     8821 ttaatgatac ccattgatag ccctctagct tatgagcctc aatccactca ggtttggaaa
     8881 tgatagtttt cctatggtat tttccattcc ttttggtgtt tatcgtttgc catttttgag
     8941 cataaaaagg atgttcaggg gtgcaaacga ttgattcagg taagccaaca gccgttaatt
     9001 ttccaactgg ttttatttca ctgccaacac gcaaaatagg ttttagttgt cctttatgag
     9061 taacaactaa atctccgaca gaaagtgttt cgattggcat atatccttta tccgttaaaa
     9121 ccatatgccc agcggtaaag caaggcgtac ccccaaccaa cacatcagga gcaggaattt
     9181 tacgattgag gattttttca ggtagggcgg tcatatcgcc caagttcggc acatcgggga
     9241 aacggtgagc aagcactgca cacggaaacg gctcaatctc gctgaaccat aacggcgtac
     9301 cgatgtcgtg ccacgctaca cttgctgcct caatgccgga gcagatagat ccataggtga
     9361 attttattat gttgttcata caatcttctc cgcctgcacc gcaacgcaaa tatcatcaat
     9421 aaatgcgtta aaagtgcggc gtaattctgg ttcaaaaaat tctgcgatca gcttggataa
     9481 gcgaacctcg ccgacaattc gttcaatcgc ttgctctcgg gcaaacgcct ctaattcaag
     9541 cggttttatc tcttcttgtg gcggtggtgg cggttggata ctttcccaat gatcaggtct
     9601 aattttccat ttatcagcac aaatttgttg aatttgccca ccagcacaat cgattaactc
     9661 ccgagagcaa tcatcggtcg ttttggtttt gtctaccacc aagaatagga cttcaatcgg
     9721 ggtatcctca aaggcgtttt ggatgcggtt gagttctaca agttgattgc caacttcttc
     9781 tcgaaagcgt ttttctgttt ggcggtagcc aacgccggga aagagaatga aaaatcccca
     9841 ttggtgacta tttgccagtc cttttaggac gaaaatatca tcaaccaccc cagatttttt
     9901 ccacggaaat tcggattgaa tttgtgcttg ttcggcttgg cttaaatcct taaatttaag
     9961 ggaaaatggc ggattcataa tcacgcaatc gcacggttcg ccgttaggat agagaaagaa
    10021 gctctgatta tagatttgag ccgtagggaa attgtgccgt aacacatcgc agctttcgga
    10081 ttggacctct accgcagtaa aagaggttgg gcggacaaat tgctccaact gcccagaacc
    10141 tgccgccccg tcaaaaacag aaacattatt tctggcatac tgcttgacct tttcagcaac
    10201 ataacggcgt agagcttcac ctgtgatgta ttcagcgaat ttgtcggctt ttttgcggtt
    10261 attgtgttct tggaattgca tttttcctcc aaaaaataac cgcttgcgga acaagcggtt
    10321 gggttaatta gcgttgagct gtacaaattt tgccatcgca gtcgttgcgt aactctagat
    10381 ggtcagcagc aactgcaaag gcggttaaaa agagtatgat ttttgcgtat ttcatcgtct
    10441 aatcctcaaa atttgggtgc aggaaaccgc cgcttgatgt ttcgcaagcg gtcgggtttt
    10501 ataatttttt gtgattagaa cgttggcttt ttaaatgccg ggtcgaaacc tcgaacgtgt
    10561 ttgagtactc gccagtttgt catcgggtcg taatcaaatt ctttggtaat ccgttcaaga
    10621 attttatgag ccgattgtgc ggtgtggcta tattcaaaac cctgtccgta gatttcaggt
    10681 gcgatatttg agccgagttt ttgaaacatc gggtagatat agcggaaagt gtgaatgcca
    10741 cgcacgaaag cgaaccaagc ccatatgagg ctttggagtt catcttcggt aaagtcgaaa
    10801 gagtatttct ttggcggtgg tgcaagcggt aggttttgct gattattttg caacgcatat
    10861 ttaccagtct tgcgaatttg tggcagaact tcttcaaata cccacgcttc aaacttttcc
    10921 gcttctggtt tgcgtgattt gataatcagt cggtataggt ttggttcgtt gatgaagatc
    10981 gctttttgtt gtctctgcat tttatcggtg aggtatcgtt tcgatacccc attttcttta
    11041 cagtgttttt gcagagtaag gctttcattt acataaccta aaattcggca aacatctgca
    11101 ccgcaaaacc acggttcttg atttggatcg ataatgacac gaacagcaga tgaattgaaa
    11161 ttgaaaacag ggaattgaac ttgatttgac attttttgta ccttttggat ttaagtattt
    11221 aatccgatca ttaagtgggt gatcggggtt caacttaccc tccaaaagat gggcggagct
    11281 tattcagatg tattgtatat cgctctctcg ccccgatcat ttcggattaa acctaaaatt
    11341 taggtacaaa aaaaccgctt tatatcggag gcggaaaccg cttttggaaa gtaagtgtgg
    11401 ttatcttagc cgaaagggcg gttttttgtc aattagattt tattcttcaa ttagcaatgg
    11461 cttttctttc agttcttcaa tcgctctggt ttgtaacagc gagctcattt gctgaatagc
    11521 caaacggttg agcattgcca gtcgctcttc ttggctgtaa ccttgctcaa ttagtagggc
    11581 gttttggctt tccaatgcag caaggacggt aagttgctca acggtagcgt ggtctcgcat
    11641 attgcctttc aggttcggat tgcggtcttt ccactgtttt gccgtttgtc cgaagagagc
    11701 ttgattgaga atgtccgctt cgctggaata aacgaaagta tgttgttttg tattcagcaa
    11761 tgccggtatg atgtgagctt tgatggcatc agtatgaatg cggtagttcg ccttgcttaa
    11821 aatgcgtttt acgctccatt cgagcttact ttcgttcgct tcttgctgtt tgagtcgttg
    11881 gaactctttg atgagataaa gcttaaattc tggactgagc caagaaccaa attcgagagc
    11941 aatatctttg tgggcgtaag tgccgctacc atatcgtcct gcttttgcta ctagcccaat
    12001 agctcccgtt gcagtccatt ttttgacaga catcacaaag cggtttaagc cgatgttgtt
    12061 tttaatttgg tgtaattcca ccaaattaaa atttggatta ttcagctctt cccaaacttg
    12121 aagaaattca atggtatttt tattttgaag ccagttttta atgagctggt cactgtctcc
    12181 aaaagctttg cacatatcag ttaggctgat gtaatcctca ccatcacgag cggttacttt
    12241 gacctccacg ccttgtacaa tcattacgct atttgccatt tcgtttctcc gtttttaggt
    12301 acaaaaaaag ccgttggtgg acggcttgaa agtgcggtta tcttaatccg aattgggggc
    12361 ggtgtcaatt agattaatcc gtataagtat ggaaccaagt taaagagtag aactacaaca
    12421 cataaccaaa aaatgaaatt catatcttgc tcagttttga ttttgtgtaa aatacgctta
    12481 agtttgtttt tcatttagga aacctttaat gtttagttta ttgcgtgatg ctttaacctt
    12541 tgctgcaatg tttacaggga ttttatttcc tgcaattatt cataaatacg atctatcact
    12601 tggtacacaa atccaattac ttagctttgt tccagctttc tttgtgttgt gccattttct
    12661 tattaagcga aaatatcaac gtgattttga cagactttcg tatttatctg ctgtggtttg
    12721 cgtagcaatg tgtgcaggcg ttggagcatt tagccaatca gaaatgattg agctaggttt
    12781 taaagttgag cgtgttcttg attatgtgac tatcaaattc ttactctact tttggagctt
    12841 gctgttattg ccagttgtgc taagaaaatt gtttgggaaa gattagttgg ttatttaaag
    12901 agagctattt gtcaaacctc aatatctatt tttgcgatct gcatcgaaaa aataaatttt
    12961 cgttctgaca gtaaaacgct ttcgtagtaa tctcataaaa aacataagg
//
LOCUS       DANLXQ010000064        12992 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.064, whole genome shotgun sequence.
ACCESSION   DANLXQ010000064 DANLXQ010000000
VERSION     DANLXQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.064"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            119..1264
                     /gene="lldD"
                     /locus_tag="PXD94_001396"
     CDS             119..1264
                     /gene="lldD"
                     /locus_tag="PXD94_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL6286259.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAAKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            complement(1318..1587)
                     /locus_tag="PXD94_001397"
     CDS             complement(1318..1587)
                     /locus_tag="PXD94_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286260.1"
                     /translation="MLSEERFGFFYIFIFIYFSLLSLSIGYIRPELRTTYDYLEIENK
                     RYYVLSIHRNNIYVLGEKTKDNDEFLFFNQDTLKYYRINITKMPN"
     gene            complement(1662..2048)
                     /locus_tag="PXD94_001398"
     CDS             complement(1662..2048)
                     /locus_tag="PXD94_001398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286261.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTSI"
     gene            complement(2301..3131)
                     /gene="fghA"
                     /locus_tag="PXD94_001399"
     CDS             complement(2301..3131)
                     /gene="fghA"
                     /locus_tag="PXD94_001399"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDL6286262.1"
                     /translation="MQQFENYRCFSGEQQVWQHFSASLNCEMKFAIYLPQSAKTQKLP
                     VLYWLSGLTCTEQNFITKSGFQRYADEHHLIVVAPDTSPRGVNVANDEAYDLGQGAGF
                     YLNATQGEWTTHYQMYDYIVSELPTLINAHFPTNGKQSIFGHSMGGHGALTIAFKNPG
                     QYQSVSAFAPIVAPTQVPWGQKAFLAYLGEDQTAWKAYDSVALLEKYRPSLPILIDQG
                     SQDSFLSDQLKPEIFCETADRLGIPYQFNLRDGYDHSYYFISSFIGEHIAFHAKHLKD
                     "
     gene            complement(3211..4269)
                     /locus_tag="PXD94_001400"
     CDS             complement(3211..4269)
                     /locus_tag="PXD94_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDL6286263.1"
                     /translation="MAYPIKSYATFSATTPLAPHSFERRELLEDDVLIDILYCGVCHS
                     DLHMARNDWGWTQSYPLVPGHEIIGRIIKVGKNAKKFQIGDLVGVGCMVDSCRECSPC
                     QHGLEQYCEVGNIGTYGGIDRFDGTPTQGGYSTCVVVSEDFVLKVSENLDTKAVAPLL
                     CAGITTYSPLRHWNVKAGSKVAVVGLGGLGHMAVKLAKAMGAEVTLFTRSLGKADDAY
                     RLGASRVVLSTDEKQMADVANTFDLIIDTVPYTHDLKPYIPTLALDGTLVLVGLVGEL
                     EQTINTVPMILGRRSISASVIGGIQETQEMLDFCAEHNIVPDVEMIDMQNINDAYERM
                     LKSDVKYRFVIDMGSLKG"
     gene            complement(4456..5391)
                     /locus_tag="PXD94_001401"
     CDS             complement(4456..5391)
                     /locus_tag="PXD94_001401"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL6286264.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQF"
     gene            complement(5671..5982)
                     /locus_tag="PXD94_001402"
     CDS             complement(5671..5982)
                     /locus_tag="PXD94_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6286265.1"
                     /translation="MSETEKQAINAPNAVMNGYLTMHYPDWFKPDGIYFNDGAFESFE
                     SSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEHYILIGGDASYSEQDMLAGNIDGVCN
                     A"
     gene            complement(5989..6381)
                     /locus_tag="PXD94_001403"
     CDS             complement(5989..6381)
                     /locus_tag="PXD94_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6286266.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFARPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSRANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNS"
     gene            6513..7073
                     /locus_tag="PXD94_001404"
     CDS             6513..7073
                     /locus_tag="PXD94_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL6286267.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKLFEKVQVQEIIEKALINRTT
                     FYRYYSGESDLVGKLIKEVKAEYQDLLKKRFESGNLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHMLLKNAFIAHAKRITSDDKNWAEKNWDYQAHIFATIALESHKYY
                     FEQGKLLPIPQVFEEWIEMVQVLKSV"
     gene            complement(7139..8128)
                     /locus_tag="PXD94_001405"
     CDS             complement(7139..8128)
                     /locus_tag="PXD94_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL6286268.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVAIPAPKAHEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVIVAIGEGVNEVAISDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFGKVQKGDFVVLGGATSSVGL
                     ASIQIAKMQGATVIALSRTHAKGDVLLEKGADFVIATSEDDVTAKLFEITNGKGVNVV
                     FDPVGRKEAAKIINAMAQDGRYIIYGALSHDDIAVPVFPILGKHLTVRGYELFEITTV
                     PEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAHEYMASNQQIGKIVVKV"
     gene            complement(8342..9259)
                     /locus_tag="PXD94_001406"
     CDS             complement(8342..9259)
                     /locus_tag="PXD94_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6286269.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTALATQAAVNGEFGRQPGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGGILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            complement(9395..10054)
                     /locus_tag="PXD94_001407"
     CDS             complement(9395..10054)
                     /locus_tag="PXD94_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6286270.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWGVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            complement(10239..11303)
                     /locus_tag="PXD94_001408"
     CDS             complement(10239..11303)
                     /locus_tag="PXD94_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL6286271.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLIIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKAQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            11394..12302
                     /locus_tag="PXD94_001409"
     CDS             11394..12302
                     /locus_tag="PXD94_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6286272.1"
                     /translation="MSDIRTLDFNLLKAFVVLLDECNVSRAAQRLSITQPAMSGILNR
                     LRESFNDPLFVRVQHGMQPTDRALQLGQTARNILQDISSMLQPPILEPEHLNMTLRIA
                     AMDYVQQIIALPLILRLRRLAPNVKVALLPVQGQNIKTLFEQKKIDLALVSRQHLSTD
                     TLKTVLYEERYVCAMSKTHPMAQTPLSLDQFCNLPFAMLSYNGGEFSGATDLALQKIG
                     LQRKVMVSVNHISLLPQLLQGSDLVAVLPERLAKTLPNVYLQPPPIEVEGFTMMMAWH
                     ERTEQDTAHRWLREVLQGVVKPKQAL"
     gene            complement(12372..12923)
                     /locus_tag="PXD94_001410"
     CDS             complement(12372..12923)
                     /locus_tag="PXD94_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6286273.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFVNMPTLPFALNVGNLFTGVFDEQGEI
                     ASETLKSSMQNFINAYAQFVEKY"
BASE COUNT         3642 a         2914 c         2828 g         3608 t
ORIGIN      
        1 aattggtttg taacaaaaat gtgatctatt tctcatattt tgcaaatagt agtaattctt
       61 atttttattt taaagtagaa tagccgcact tttttcttaa tgatttatga ggttttaaat
      121 gatcatttca tctgcaaacg attaccgccg tgcggcaaaa cgtcgtgttc ccccttttat
      181 gttccattat gcggatggcg gttcttacgc cgagcagacc ttacgtcgta atgtgagcga
      241 tttggaagac atcgccctgc gtcaacgtgt gttaaaagat atgtcgcaac tagacaccgg
      301 catcgagctg ttcggcgaaa aactgtctat gccggtgacc ctcgcccctg tcggtgcatt
      361 aggaatgtat gcccgccgtg gcgaagtgca agcggccaaa gccgcagata acaaaggtat
      421 tccgtttacc ctttccactg tctctatttg cccgattgaa gaggttgcac cggcaatcaa
      481 acgcccgatg tggtttcagc tctatgtgtt aaaagatcgg ggctttatga aaaatgcatt
      541 ggaacgagcc aaagccgccg gttgttccac gctagtgttt accgtagata tgccgacccc
      601 cggcgcacgt tatcgtgata tgcactccgg aatgagcggc ccatacaaag acattcgccg
      661 tgtgttgcag gcaatggttc acccgttttg ggcgtgggac gtgggcattc acggtaaacc
      721 ccacacgtta ggcaatgtgt caaactatat gggtaaaccg attgatctga acaactacat
      781 cggctggctg accgataatt tcgacccatc aatttcgtgg aaagatttag agtggattcg
      841 tgaattttgg gacggcccga tggtgatcaa aggcattctc gacccagaag atgctaaaga
      901 tgccgtaaga tttggtgcgg acggtatcat cgtctccaac cacggtggtc gtcagttaga
      961 tggggtactt tcttctgcca aagccctgcc aagcattgcc gatgcagtaa aaggggagat
     1021 caaaatcctt gccgactctg gtatccgcaa cggcttagat gtggtgagaa tgctggctct
     1081 cggcgcagat tgtacgatga tcgggcgttc ctttgtttac gctcttagcg ccgccggtca
     1141 agccggtgtg gaaaacctgc tcgacatctt ccttaaagaa atgaaagtgg cgatgacact
     1201 taccagcaat gctaaaatta gcgacatcgg cagagatgct ttggtagatt taagtaatct
     1261 ttagctcaaa tagccacaat ccgcacgttt taggtgcgga ttgttatttt agataggcta
     1321 atttggcatt ttggtaatat taatgcggta atatttcaat gtatcttggt tgaaaaagag
     1381 aaattcatcg ttatctttag ttttttcgcc cagcacataa atgttattgc gatgaataga
     1441 taagacataa tatcgcttat tttcaatttc aagataatca tacgttgttc tcagctcagg
     1501 gcgaatgtag ccaatactca aagaaagtaa gctaaaatag atgaaaataa aaatatagaa
     1561 aaaaccgaag cgttcttcgc ttaacatttt ttgaatatct aagtggaata cgcggtggcc
     1621 ccaatgtttg tgaaataata atactgatag ggtagttgtc cctagataga ggtagttgtc
     1681 cctagataga gtaggtgtaa atacattggc actttgccaa taaacagata gaaactgatc
     1741 attactggca agctaaaaat agtaacaaga acactcaccc gtagccagcc aaggtattta
     1801 aagtaatgca gcttaaaaac tttattaact aaagcgtatc ctatcaaata aaaaagaaag
     1861 atagttaaaa atgtgccaaa aacgtaggct agtgcgcgag ctataccggc attaccaacc
     1921 tcaacatgcc accacgggta gccatgaaaa gcagcaatac cccaagcata acagtaagcg
     1981 gtagaaaaag agattaaagt aaagaaagcg agagtaagag aagagttgat gaatttctga
     2041 ttagtcataa cagtaaatag attgcaccta cttagttaaa gatgaaacac aagaaagcag
     2101 actgagttta gcttccaccc aactcctttg tttagaggag aactttatta aagcacgact
     2161 tttgttgtat ttcaaacctt tgtttttata caaaacaacc gtgttgtaaa aaatgatcgt
     2221 aaactgaccg cttgtaatgc actctgaata tatcgaaaca tgataaaaat gataaagcat
     2281 gtttcgataa tttactgtct ttagtctttt aaatgttttg catgaaaggc tatatgttca
     2341 ccgataaagc tactaatgaa atagtagctg tgatcgtaac catcacgtaa attgaattgg
     2401 tagggtattc caagtctatc tgcggtttca cagaaaattt ccggctttaa ctgatcgctc
     2461 aaaaagctgt cttggctgcc ttgatcgatt agaattggca agcttgggcg atatttttcc
     2521 agcaatgcca cgctatcata cgccttccaa gcggtctgat cttcgcctaa atatgctaaa
     2581 aatgcttttt gcccccaagg aacttgggtt ggggcgacaa tcggtgcaaa tgccgataca
     2641 ctttgatatt gccccggatt tttaaatgca atggttaagg caccatgtcc gcccattgaa
     2701 tgcccaaaaa ttgattgttt accgttagtc gggaaatgag cattaatcaa tgttggcagt
     2761 tcgctgacaa tgtaatcata catttggtaa tgtgttgtcc attcgccttg cgtagcattt
     2821 aggtaaaatc cggcaccctg acctaaatca taagcctcat cattagcgac attcacaccc
     2881 ctagggctgg tatccggtgc gaccacaatc agatgatgtt cgtcggcata acgttgaaag
     2941 ccagatttag tgatgaagtt ctgctcggta caggttaaac cagaaagcca atatagtacg
     3001 ggtagctttt gtgttttagc agattgcggc agataaatag caaatttcat ttcacagttt
     3061 aagctggctg agaaatgttg ccaaacctgt tgttcaccgc taaaacagcg gtagttttca
     3121 aattgttgca taatattcct tacaagcggt ttaattccgc tgattttttg caaaaataaa
     3181 ggcggaactc accgccttta tattgtaata tcaacctttc aaacttccca tatcaatcac
     3241 aaaccgatat ttcacgtctg atttcagcat tcgttcataa gcatcattga tgttttgcat
     3301 atcaatcatt tccacatccg gcacaatgtt gtgttcggcg cagaagtcga gcatttcttg
     3361 agtttcctga atgccgccga tgacagaggc ggaaatagag cggcggccta aaatcatcgg
     3421 tacggtgttg atggtttgct ccaactcacc gaccaagccc accagcacca atgtgccgtc
     3481 caacgctaag gttgggatat aaggtttcaa gtcgtgggta taaggcacgg tatcgatgat
     3541 taaatcaaag gtatttgcaa cgtctgccat ttgcttttca tcggtagaaa gcaccacacg
     3601 gcttgcgccc aaacggtaag catcatcggc tttacccagt gagcgagtaa aaagcgtaac
     3661 ttccgctccc attgctttgg caagtttaac cgccatatgc cccagtccgc caagccccac
     3721 aacagcgact tttgagccag ctttgacatt ccaatgacgc aggggcgaat aggtggtaat
     3781 gcctgcacac aaaagagggg caaccgcttt ggtgtccaag ttttcagaga cttttaatac
     3841 aaaatcttca ctcaccacca cgcatgtgct gtaaccgcct tgtgttggtg tgccgtcaaa
     3901 gcggtcaata ccgccataag tgccgatgtt gcccacttcg cagtactgct ccaaaccgtg
     3961 ctggcaagga ctgcattcac ggcaggaatc caccatacaa ccgacaccga ccaaatcgcc
     4021 gatttggaat tttttggcgt ttttaccgac tttgataata cgaccgatga tttcgtgccc
     4081 tggtacaagc ggatagcttt gcgtccagcc ccaatcattg cgagccatat gcaagtctga
     4141 atggcacacg ccacagtaca aaatatcaat caaaacatcg tcttcaagca attcacggcg
     4201 ttcaaaactg tgtggggcaa gtggtgtggt tgccgaaaaa gtggcgtagg atttgatagg
     4261 gtaagccatt tttggggttt aaatggctga aatggcaatt actatagtgc cgatgattta
     4321 ttattgaaaa tcaagatttt tgataaatcg gtatcagtta aaatgataat tgggggtttt
     4381 cagactgact gaaaaattat tttgcccatt ttaaaaacaa aaacaccgcc ctcatcacaa
     4441 aagcggtgtt tttcatcaaa attgtgtcaa agcatactct gccaattctg taagcacttc
     4501 atcagcagga cgctgagtgg gttttaaatt gagtgccaaa tgtttcacgc ctttttcttg
     4561 ttcttttgcc caaaagtctg ccaagccttt tgcaccacca cgcagataaa tgttgttaaa
     4621 taactgtgct ttggcgtttt tgtcgtccaa aacttccaca aattgagcat agccaaatgc
     4681 tgtgggggtg tctttttggc gtaaatcggc aagggtgttg atgatgtttg cggtgtcggc
     4741 aggattcaca ccgtgccaaa tccacgcatc aggtacatta gcaagccagt ccatttcttg
     4801 ccttgctcgt ccaatcgcca caatgggcaa gcggtgtttg ggtttgggga gcaaatccaa
     4861 attgtctgtt aaatcggcat aatgtttgcc tttgaaatgg ggaaaatcct gctcggttaa
     4921 ggtgttaatc aaatcaatgc tttcacgaaa acgctcgcta cgagaggcaa aatccgcccc
     4981 aaatgcaggg tattccacag gtctatcgcc agaggataag cccaataaaa accgtccatg
     5041 actcaaacta tccacacttg tcgccatttt ggcaatgtga atgggactac gcaaaggggc
     5101 aatcagccct gccgtgccaa gggcgatgtt ttgggtgtgg gcggacaaaa aaccaagtgt
     5161 aaccatcggg tcaagccctt gccccacatc gccaaaatga gggtcataaa acggcacatc
     5221 tcgtacccac aatgctgaaa agcccaagtt ttcagccagc cgtgcattgt cggcaatggt
     5281 ggtcaaattg tctggaatgt gattttgata gcccataaag ggggcaatta agcccagtgt
     5341 taattgattt tgcccaaaaa cttgggagaa aatgggggaa agcgtgttca ttttggcgta
     5401 cctgtcttat ttaagataag gtattttaag ggcttttgag tttgcttaaa atgggataaa
     5461 ttgagaaagt gttttgctaa attggcataa atagacgctt tcaagctgac tgaaaatttg
     5521 caaaattttt aataaaaatg accgcttgcc tttcaacaag cggtcattcc cctttaattt
     5581 tttaccaccg taaacacctt atttttcagc cgatattcac ttttttcatc gtgagccagt
     5641 tgcacaatgg ttggcttgcg tttacacaat tcacgcattg cacacgccgt caatgttgcc
     5701 agcaagcata tcttgttcag agtaagaggc atcgccgcca atcaaaatat aatgctcgcc
     5761 catatccacc accaccgcca aatgccccag cgtatgtcct gcggttggca caaggcggat
     5821 tttgccgtct ttggtgagtt tatgactgct ctcaaaactt tcaaacgccc catcattaaa
     5881 atagatgcca tcaggcttaa accaatcagg atagtgcatt gttaaataac cgttcatcac
     5941 cgcattgggg gcgttgattg cctgtttttc agtctcactc atcacaaatc agctgtttgg
     6001 gaaattgggc aatccgcctg cgtggtcgcc gtgcatatgg gtcattacca ccgtgtcaat
     6061 gcgtaaaggg tcaaagccgt ttgcctttaa aatttcgccc acttcttcgt gttttttgac
     6121 cccacgccgt tcgcaaaact gcataaacgg gtgccaccac ggctgatagc ccttgtcgtt
     6181 ggcacgactg ctttcgcccg tatccaccaa aatcaaaccg tcatcgtgtt caatcagcca
     6241 gcaaccaatc ggcagtttag gcgaccattt tttatcggtc agcacatcaa gcacacgggc
     6301 aggacgggca aaaaagcgtg ccacttgatg atgaattttg atttgtaccc aacctgttgt
     6361 cagacaatgc actttaatca tttttgtgtt ctcattaaaa aatcgtgggc atattgtaga
     6421 aagtgcggta acttttcaca aacattaaaa agttcgtatt tggctggata tgcagataaa
     6481 attacaaaaa tgtatgctta ttttgaaaaa agatgaacga tttaagagta atcaaaaccc
     6541 atctagcaat ccaaaacgct ttgattgaat tattagatga aaagctgttt gaaaaagtgc
     6601 aagtccaaga aatcattgaa aaagccttga ttaaccgcac cactttttac cgttattatt
     6661 cgggcgaaag tgatttggtc ggcaaattga taaaagaggt gaaggcggaa tatcaagacc
     6721 tgctcaaaaa gcgttttgaa agcggtaatt tgctgcgatt tatgcaaacc attggcaacc
     6781 gcttattgga aaaaagacgg ctgattttgg cactttggca aatcaaaacc cctcgccatc
     6841 atttgtatga cgatatgcac atgctgttaa aaaacgcctt tatcgcccac gccaaacgga
     6901 taacatcaga tgataaaaac tgggctgaga aaaattggga ttaccaagcc catatttttg
     6961 ccaccattgc ccttgaaagc cataaatatt attttgaaca aggcaaactt ttgcccattc
     7021 cacaggtgtt tgaggagtgg atagaaatgg tgcaggtgtt gaaaagtgtt tagaaataac
     7081 tggtaaaatt ttccataaaa aacaccgctt tcatcacaaa agcggtgctt tcaaatcatc
     7141 aaaccttaac cacaatctta ccaatctgct gattgcttgc catatattcg tgagctttaa
     7201 ccatttcatc aaaggcaaag attttgtcaa tttcagggcg taactgaccg cttgccaaac
     7261 cgtcatagac aaaggttttg gcttgtgcca atttttcagg tacggtggta atttcaaaga
     7321 gttcataccc ccgcacggtc aaatgcttac ccaaaatcgg gaacactggc acagcaatat
     7381 catcatggct taacgctcca taaatgatgt aacgaccatc ttgtgccatt gcattgatga
     7441 tttttgccgc ctcttttctg cccacagggt caaataccac attgacgcct ttgccattgg
     7501 tgatttcaaa aagtttggcg gttacatcgt cttcgcttgt ggcaatcaca aaatccgctc
     7561 ctttttccaa aagtacatcg cctttggcgt gggtgcgaga caaggcaatc acggttgccc
     7621 cttgcatttt ggcgatttgg atactggcta aaccaaccga actggttgca ccgcccagca
     7681 ccacaaaatc gcctttttgt actttgccaa actcaatcag tccgccataa gcggtaacaa
     7741 acgccatcca actggcggac gcttgttcca ttgacaaatt atcagggtgc ttgaccacag
     7801 cgtgttttgg catattgacg atttcgccat aagtgccgta ttcggtaaac ataaaggacg
     7861 gaataatgct caccttatcg ctaatggcaa cttcgttcac accctcgcca atcgccacaa
     7921 tcacaccagc cccttcataa cccaaagtcg cagggaatac agggtcaatc acatacgccc
     7981 cttcacggta catcatttcg gcacggttta agccaatggc ttggatttgg atttgtactt
     8041 cgtgtgcttt tggtgcagga attgctacat caacgatttg caagacatca ggcgaacctg
     8101 tttttgtaaa ttggatttgt ttgctcattg ttttgacctt ttgtgtaaat aatgggcgta
     8161 ttttaatgca atgaatttat tttaaacaca acatcaattg caaaacactt ttgctaaatt
     8221 tacataaata cattttcaca atgtctgaaa agttgttttg caaattttaa aaagaaaacc
     8281 accgcttgcc gatcacaagc ggtggttttt gtgggttatt ttgcaagagg tgtcagcttg
     8341 gctacagtaa gcggataaat gcgacgccgg ctaagagtaa ggtaacgccg atgatctgca
     8401 gcggtagaat gggtttttgt gtaatgccga acagaccgaa tttatctacc agcaggctgc
     8461 cgcaaatcag gccagagagg acgagcatca ccaccgagcc tgtgccgatt tgggggacga
     8521 gccaaacgcc agcgaggata aacaggctgc ccagtatgcc gccgctccat atccaaaacg
     8581 gtttgccttc ggttggctgg cagagtttcg gcaggctttt ttcataaaac agcactacca
     8641 gtaataagcc gatggcaccg acaccgaagg agactgttgc tgcgccgagt gatgagccgg
     8701 gctgtctgcc gaattcgcca ttaacggcgg cttgggttgc taatgcggtt ccgcctaata
     8761 tgccgactgc gcgccacagc cataaatttg cgttttcttt gcgttttaga ccggacagat
     8821 ggggcagtac cacggcaatc catacgccga ataagactaa accaatgcca aacaggcggc
     8881 tggccgtgaa ggctatgtaa ggcgagttaa gccaagcgaa agtgtcgatc agcacgccca
     8941 tcagcacttg gcctaaaatc ggcatgaccg cagtttgtac gccgccgagc ttggggaaga
     9001 tcagtacgtt tgccgttagc cccaccatgc cgagtaatcc gcccgtccat gcccataagg
     9061 ggacgttgcc taaattgtcg aggctaaatg ctggtgtttg ttgcatgatg agcgagagtc
     9121 cgagtagaaa caggaggcct acgctgaaag agacaaacga ggttaaaaat gccgcaccca
     9181 cgtgcatacg caaacgggcg ttgatggctg attgaaatgc aatcagcacg ccggctaaaa
     9241 taccgataat aaatgtcatg actaactggg ttgtgctgcc gcctcaaaat gagtggtggt
     9301 tgaaaagaaa tgccgtctga atgcggattc agacggcctt aaggcttggt atcggcgttt
     9361 acctgatgtt gaatagccgt tttgcctagc cggtttattc gatgaatttc accacggttt
     9421 cgaaatcttt acgtgctttt tggcggattt ctttatcacg gtaaccgaag gtacacatca
     9481 ccgatacgcc ccattcgctt gggtcaaata atccttccgc tgccaaaata tcgttgactt
     9541 cttggtaagc gaaaccttct atcggacaag aatctacgcc aattaaagct gcaccggtca
     9601 tcatattgcc caatgcgatg taggtttgtt tgcttgccca gtcaaacagg ctgcgttcgc
     9661 tgtctaaaat gccgatatct ttctcttgga attttttata cacggccatg gctttttcgc
     9721 gcgcttcgcc ttgtaaacct cgtctatcca taatttcaga aaaatattgg ctgtcgtaac
     9781 gggcattttt cttcgctaaa atcaccacga tatggcttga ggtttccata gttggaatgc
     9841 cccagcagta aggtttgatt ttttgccgta attcagggtt ttgcaacact aaaaactgcc
     9901 aaggctctga gcctaccgaa cttggactta aacgaccgag ttccagaata tagttgaaat
     9961 cttcagccgg aatttttctg ctgccgtcgt aagaacgggt agatgcacgg taatgaaagg
    10021 cattgattac gtcttctttg ttgaaaaatt gcatggaaca ctcctaaaaa tgcaaaacag
    10081 tcttttcaga cggtctgaaa agtctagatg aaatacggat aagtataaaa gatttaagtt
    10141 atttttaata attgataatt ttgatattta ggtataagtt gacgtgatga gaaaaaacag
    10201 accctctgaa tgttcaggcg gtctttaacg ttggttgttt acataaataa tccgcgttca
    10261 tattgcttcg gtacaaactg catttcactg ctgtctgctg cattttggtc atattgcgca
    10321 ttcaataccc agtacggatc gcgcaataag gctcttccca cagccactaa atccgcatcg
    10381 cccataccta agatatgatc gcctaccgcc ggattatcca acatacctac tgcaattacc
    10441 ggtttgcccg tagcgagctt cacttgacgg gcaaagtcta cttggtaacc tgcgtaaaat
    10501 tcggggtgat tacctgcatg caatacacca tcaccaccac cgctgatgtc taagacatct
    10561 gcgcctgcat cggcaaaacg tttcgctacc tgcaaaccat aatccaagcc aaagccgttt
    10621 tcaccgtatt cttgtgcaga aatgcggaca attaacggca tttcagacgg catcacttca
    10681 cgtgctgcct gaatcacttg ctcgccaaac aacattttgt cttggccgta ctcatcactg
    10741 cgctgattgg atttcggcga atagaattgg tgaattagat aaccgtgtgc gccgtggatt
    10801 tcaattgtat caaagcctgc ctcaacggca cgtttgaccg aatccttaaa ggcttgaacc
    10861 aatgctttga cttcgtctgt cgtcagctcg cgaggtgtaa tcagattttg tccggcataa
    10921 tccaactcac cgtagtgaat tgcacttgga gccactgcat ccggctcgtc ttgggctttg
    10981 cgtccggcat gggcgatttg cacaccgatt tttgcccctt gagcatgaac gctgtctacc
    11041 agttttttga atgcctgttg ttgttcatcg ttccataagc ctaagcagtt tggcgtaatt
    11101 ctgccgtttg gcgccacatt ggtcatctcc acaataatca agccgacacc acctactgca
    11161 cggctggtgt aatgcacgaa atgccaatcg ttcggtacgc cgttggttgc cgaatattga
    11221 cacattggtg gcataaccac acggtttttc aatgctaagt ttttaatgtt aaacggggtg
    11281 aataattttc tgaacttcgc catgttgcct tccttaatga aatagattag gtggaatgat
    11341 aattcagtgc tattatctat tcaaattgat tattgtgata gttaggtata aaaatgagtg
    11401 atataagaac actggatttc aatttgctca aagcttttgt ggtgctgctg gacgaatgca
    11461 acgttagccg ggcagcacaa cgattatcca tcacccaacc ggcaatgagc ggtattttaa
    11521 accgcttgcg cgagagtttt aacgatccgt tatttgtgcg agtgcaacac ggtatgcagc
    11581 caaccgaccg cgcgttgcaa ctggggcaaa ccgcacgcaa cattctgcaa gatatcagct
    11641 ccatgctgca accacccata ttggaaccgg aacatctgaa tatgaccttg cgtattgccg
    11701 caatggatta tgtacaacaa attattgcct tgccgctgat tctgcgttta agacggcttg
    11761 ctccgaatgt aaaagtcgcc ttactgccgg tgcaggggca aaatatcaaa actttgttcg
    11821 agcaaaaaaa aatcgatctt gccttggtca gccgccaaca tttaagcacg gatacgctga
    11881 aaacggtatt atatgaagaa cgctacgtat gcgcgatgag caaaacacac ccgatggcgc
    11941 aaacaccgct atcgctcgat caattctgca atcttccctt cgcaatgctt tcttacaacg
    12001 gcggagaatt cagcggagct acggatctag cgttacaaaa gataggacta cagcgcaaag
    12061 taatggtctc ggtaaaccat atttccctgc tgccccagct gctacaaggt tccgacttag
    12121 tcgcagtatt gcccgaacgc ttagcaaaaa ccttacccaa tgtttattta caacctccgc
    12181 ctatagaggt agaaggcttc accatgatga tggcttggca cgaaagaacc gaacaagata
    12241 cggcgcatcg atggttaaga gaggtgctgc agggtgtggt aaaacctaaa caggctcttt
    12301 aattattctt tcttttgcta tgaaaaaagc cgtttatatt tttagataaa cggctttttt
    12361 gcattctaat attagtattt ttctacaaac tgagcatagg cattaatgaa gttttgcatt
    12421 gagcttttta atgtctcaga ggcaatttca ccctgttcat caaatacgcc tgtaaataag
    12481 tttcctacgt ttaatgcgaa aggtaaggtt ggcatattta caaatgagcc tgaagtaata
    12541 gtacgtactt gatcagctgc acgtacaccg cctgccgggc ttgcgccaac ggttacgata
    12601 cctgccggtt tgccaatcca tttgctttgc cccattgggc gagaaaccac gtcaatcgca
    12661 tttttaagca ttgcagaaaa gcttgcatta tgctcaggtg tgactaataa cacgccatct
    12721 gccacagcaa ccgcttcacg cactctggtg tattgtgccg ggctgttttc atctaaatca
    12781 cggtcgtaaa gtggtaagtc tgcaatatcc acaatgttta attgaatgct tgcaggtgcc
    12841 attttttgta agtgacgaac agtcaagtga ctaaatgaag ttttgctgcc gctaccaatt
    12901 aatactgcaa tttgttttgc cattttataa ttccttagta catagagaat ataggcattt
    12961 tatccctttc aatctgtttc ataaagcgct ta
//
LOCUS       DANLXQ010000065        12921 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.065, whole genome shotgun sequence.
ACCESSION   DANLXQ010000065 DANLXQ010000000
VERSION     DANLXQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.065"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..212)
                     /locus_tag="PXD94_001411"
     CDS             complement(<1..212)
                     /locus_tag="PXD94_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6286274.1"
                     /translation="MPKSWELSLTARSNVIEILQSVEDYTGHWLSAVSLWEQLQNKLD
                     LIAFMPTIGRRREDGTLETFCRNYRIV"
     gene            complement(205..396)
                     /locus_tag="PXD94_001412"
     CDS             complement(205..396)
                     /locus_tag="PXD94_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286275.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
     gene            complement(686..1276)
                     /gene="yraP"
                     /locus_tag="PXD94_001413"
     CDS             complement(686..1276)
                     /gene="yraP"
                     /locus_tag="PXD94_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL6286276.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSAAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNSEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            complement(1286..1870)
                     /locus_tag="PXD94_001414"
     CDS             complement(1286..1870)
                     /locus_tag="PXD94_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL6286277.1"
                     /translation="MLNKIQDRFTESIQTQISASELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            complement(1876..2247)
                     /locus_tag="PXD94_001415"
     CDS             complement(1876..2247)
                     /locus_tag="PXD94_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL6286278.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DPIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            complement(2222..3943)
                     /locus_tag="PXD94_001416"
     CDS             complement(2222..3943)
                     /locus_tag="PXD94_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL6286279.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            4017..4889
                     /gene="rsmI"
                     /locus_tag="PXD94_001417"
     CDS             4017..4889
                     /gene="rsmI"
                     /locus_tag="PXD94_001417"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL6286280.1"
                     /translation="MKNRQNSSGINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            complement(5134..6300)
                     /locus_tag="PXD94_001418"
     CDS             complement(5134..6300)
                     /locus_tag="PXD94_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6286281.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGITVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(6452..7966)
                     /gene="putP"
                     /locus_tag="PXD94_001419"
     CDS             complement(6452..7966)
                     /gene="putP"
                     /locus_tag="PXD94_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL6286282.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMIICLLGAIGIGYFGMAYFFGNPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            complement(8060..8848)
                     /locus_tag="PXD94_001420"
     CDS             complement(8060..8848)
                     /locus_tag="PXD94_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL6286283.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            complement(8845..9423)
                     /locus_tag="PXD94_001421"
     CDS             complement(8845..9423)
                     /locus_tag="PXD94_001421"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL6286284.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQVSHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            complement(9425..9616)
                     /gene="ssrS"
                     /locus_tag="PXD94_001422"
     ncRNA           complement(9425..9616)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PXD94_001422"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(9721..10011)
                     /locus_tag="PXD94_001423"
     CDS             complement(9721..10011)
                     /locus_tag="PXD94_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL6286285.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVVVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            10090..10647
                     /locus_tag="PXD94_001424"
     CDS             10090..10647
                     /locus_tag="PXD94_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL6286286.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            10745..11809
                     /gene="hemE"
                     /locus_tag="PXD94_001425"
     CDS             10745..11809
                     /gene="hemE"
                     /locus_tag="PXD94_001425"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL6286287.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            11956..12546
                     /locus_tag="PXD94_001426"
     CDS             11956..12546
                     /locus_tag="PXD94_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL6286288.1"
                     /translation="MRNPIHKRMERFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
     gene            12682..>12921
                     /locus_tag="PXD94_001427"
     CDS             12682..>12921
                     /locus_tag="PXD94_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL6286289.1"
                     /translation="MNKTELIDAIAAGANLTKKDAKAALEATLDAISASLKAGDAVQL
                     IGFGTFKVNERKARTGRNPKTGEEIKIAAAKVPAFV"
BASE COUNT         3829 a         2833 c         2618 g         3641 t
ORIGIN      
        1 acaatacgat aattgcggca gaatgtttct aatgtgccat cttctctacg tcgccctatt
       61 gtaggcataa atgcaatgag atcgagttta ttttgtaatt gttcccaaag tgaaactgcg
      121 ctcaaccaat gccctgtata atcttctacc gactgtaaaa tctcaatgac attagacctt
      181 gcggtaagtg acagctccca agatttaggc ataattaacc tcaaacaaat tttcattttc
      241 tcgtaattgc tgacgtttct gcatatccgc aaataaatga tttaattcgt tattgatttg
      301 ttctttggta aaggtttctc ctcgagctat tgcttcttga ccagctttaa tttgctctgc
      361 aagaaactca tcatatcctt gttctttgaa tagcatattc tgctcctttg atttgtgtct
      421 tgattccact atgttcaaga catagtttaa gtttaaatct acgctcaaag caatgaagag
      481 tcaagggaat ccttatgttg atagggtgtg catatttttc gagtcaaatg aattaaaaat
      541 catcattacg atcacccact catagatttt atcttcctat ctaaggtcat cttcttatat
      601 caaacaagcg gtctaaattt gcaaaaaaat gacaaattta gaccgcttgt gagttacaaa
      661 aatataaact aattgagctt atcaattact gcgcataact aaatactttc accactttgg
      721 ttactccacc tacattgcga gcggcttcgg cggcggcatc agcttggttt tggctgactt
      781 tgcccattaa aaacacttcg ccgttttcgg tgacgacttt cacttcggtg gcttttacct
      841 cgctatttac taacagtttg gatttcactt gggtggtaat ccagctgtct tgtgtaatct
      901 gtccaaagcc gattttttcg ccttggcgga tttcgttgta aatttccgtc acgccctccg
      961 ccccagcagc gaggtttttc gcattttcca ctacgtccat acttggtgct tgcccgatta
     1021 ataacacttt gccgttgtaa gccaccacgt taatgcgggc ttcttgtttg atttgctcgt
     1081 ctttattaat gttgtaagcg actttttctt ctaaaatttc atcatcaact tgcgtgccgg
     1141 tggtgcgtgg gtctgtcgcc actttagttg caaccgccgc agaagtcacc accgccgtgg
     1201 tgatacaacc ttgtaaagag ataaaaccta atactaatag gctgcccttt aatagtttat
     1261 ttgctaattt gttcataatc tctccttacg aaaatagtaa atggtcgata agttcacata
     1321 ataaattcac ataaaaatga tggctttcaa tcaccctcag ttgattggtt gaaggggctg
     1381 aaatttcaat atcactttca tctaataagc cgagtgtgtg gtcgttatta ctgcctgtta
     1441 aagcaataat atctaaatct aaatgttgat ttttcgccgt atgaatagct tgcaatatac
     1501 attcttcatt acccacaggc gaaaaaacaa tgaagagatc accctctttt gcaaccgcct
     1561 gcaactgttt tttataaaca gcctctaact cgttatcttg tgccacaact cctgccaata
     1621 caccgcttaa ttgcagttgt acggcagcaa aactcggtct tgccagttca taacggtgaa
     1681 gtaagtggct gaccaataat tcggcattac cgtaagagcg accgtgtccg cagacgataa
     1741 ctttatcgcc acgcaacaaa caggcaacaa tgcggtttgc cgcttcactt aatttagccg
     1801 gcaatagctc agaggcggaa atttgggttt gaatactttc ggtaaatcta tcttggattt
     1861 tatttaacat tctttctaac ctaaaaagtt gggaatccac acaggcgttt ggtttccttc
     1921 aaaagcaacc acatcaaaac ggcaatttgc agtttctaaa ctttggtttt gtttaagcaa
     1981 ccacatatta gcggcatttt gccatttttg ttgcttgcgg taatcaatac tctcaacggc
     2041 tgaaccgaag cggttgcttt tacgctggcg aacttctaca aacacaacgg tttgtccgtc
     2101 ttgcataatc ggatccagtt cgccaccttt aaatgcttgg ttagcggcaa caaaacgaag
     2161 cccttgctgt tctaaaaaca ctcgggcttt ttgttcataa aacgagcctt gttcccgttt
     2221 attaataagc ttgttgaata gctccattgc gatattgcat ccaagataaa ctacgttcaa
     2281 tattacaatg ttgtccggct tttaactcac cggttaaacc tgaaattttg tagcccggaa
     2341 tatggcggaa ttcattgaat ttggttgcca atgaccacgc atcagccccc attgcgtata
     2401 aacgcatcat tgagaaatcg ctattggcta aattctcggc ttttttatat tcactagaac
     2461 tttgatcagc taacagtggc acttcactaa attttacccc ttccattgaa gtgtaaaaat
     2521 caatgccgtt atttggcgaa ttactgcgag atgaggtata gatcgctaaa cgatctttta
     2581 aggttgagct atcaatacct tgtttaattt ctaatacttg ctcggcattc cccaaaatat
     2641 ataaagctgc tttgctcaca cctgcatttt gaatagcggc aacggcatca agcggttggt
     2701 tgtagtaacg cacatcagca tcgctattgg taagctgacg ccagcgtttg gcaaaggctt
     2761 ctgccgagcg gttgccgtag tcatcttgag gggccgcaac gatagcgaca ctattaccgt
     2821 cttgataaat tttttccgcc cctgattgtg cctcagattc aggagataaa ccgtagtaac
     2881 acacctgagc aatattacgc acattttcag tggcattcag tgccaaaata ttcacgtttt
     2941 ggatttctgg gctaagtagc atttcatcaa cacgagattt gagcaatggc ccgacaatca
     3001 catttgcccc ttgctgtttt gcctgtgaaa ttaaggaatt aacatcagat gaatcggtat
     3061 caaaagtttg cactgcggtt gagtcatcag ccttagcatc attaaaacca cgcttgataa
     3121 tatcacccaa aatttttgca tcaccactta gaggcaatag caatgcgaca ctgtttaact
     3181 gggtttgttg gaaattactc accccttgta attccgttgg cattacggca accgcactat
     3241 ggctcgggta ttgggctttc caattattaa ttgcttgtgg catttgtgcc ggtgtcgtca
     3301 cattttgatt atagatatta attaatgcca accaaccagc aagctccatc tcacccgctt
     3361 caggcacagt tttttccagc attcctcgat ttgcatcacg caaggtttgc caaataatat
     3421 cgttattttc ttggcgttca cggttcattt tatagaaatt gttaagttgc gatcttgcac
     3481 gcacaatgcc aatcacatct ttctgattgg cagcaatacg ggcttgggtt tgaatataac
     3541 gcaaacgctg tgccgaactt aatagcggat cttgaatggc ttttaattgg cttgttgctt
     3601 gctcgttttt cccttgtaag gcggcaagct gggcggaaac caaggcatac tctaactttt
     3661 gcacctcgtt catctctgcg gtcagaattt cactaaaggt attttgagcc tcatattcct
     3721 tattttcatc aattaatttg cgtacagcta aaagttgata cgaaatttta tcttccgctt
     3781 ctttggtttg atctgcttta ttaatataga attccgagct tgaataggct tcatttttaa
     3841 tggattcggt gacaggattt accgatgtac aggcagaaat gaataatgca atcgccgttg
     3901 gcaccaatgc ctttttcatt gtatggttta aaatagttgc cattgtcaga tcctcaatag
     3961 aatataatgt gctaaattta atgtgaaatc ttacttatca aacgagctaa aagcaaatga
     4021 aaaacagaca aaattcatct ggaatcaacc caaattacgg aattttatat attgttgcca
     4081 cgcctatcgg caatttaggc gatattaccc aacgggcgtt agacaccctt tcatctgtcg
     4141 atttagtcgc cgccgaagac actcgccaca gcggtttatt gctgagccac tacggcatta
     4201 aaaaaccgtt tttcgccttg cacgaccata atgaacagca gaaagcggtc gttttagtcg
     4261 aaaaattagc aaaaggggaa aacattgcgt taatttccga tgccggaacg ccactgatta
     4321 gcgacccggg ctttcatctt gtccgtcatt gccgccaagc cggggtaaaa gttgtgccga
     4381 tccccggggc ttgtgccgct gttactgcct tatgtgcttc aggcatagct tcagaccgtt
     4441 tttgttttga agggttctta ccggcaaaaa caaaatcccg ttgcgataaa cttaccgagc
     4501 ttgaaaacga gcctcgcaca ctgatttttt atgaatccac ccaccgtatt ttagacactc
     4561 ttgccgatat gcagactgtt tttggcgaag atcgctatgt ggtaatggcg cgtgaaatca
     4621 cgaaaacgtg ggaaaccatt tatggagatc aactgggtaa cttaattgaa tggctaaatg
     4681 aggatagcaa ccgaattaaa ggcgaaattg ttctggtggt tgaaggtaaa ccgcaaagta
     4741 gtgaggaagc cttttccagc caagcgatca aattactgac attactctgc caagaacttc
     4801 cgcttaaaaa agccgctgcc attgtggcag aaaccttcgg ctataagaaa aatgccctgt
     4861 atcaatatgg attggagcat tttgagtaac agacaagcgg tcagattttg tgagtttttt
     4921 gcagattggc actcccctct ctctgaaaaa tgtttctgaa cgaaacattt cccggtctca
     4981 atactcgcta cgctcgctcc ttcggagccg ttggcaaagc caacgttcaa atgtaaacat
     5041 ttgtcccata aggggagaga gctaaattat tcgcttgtcg acccctctcc actcgtggga
     5101 gagggacaga ttcttcttcg ttcagaagaa aaatcaggga gagggggaag tttctcgcac
     5161 taaattccga taagcatatg ccataatcgc aaacatcaac agcaagtaaa gcggtaataa
     5221 ttgaatgctg atagcattag caataaagcc gaaaatcggt ggcaataaca ggacgccgat
     5281 ataggcactt gccatttgca cgccgatcac cgattgagac ttgtcctcgc cgaaaaacgc
     5341 aggggtcgaa tgaattaagc acgggaaaat cggggcacaa cctagcccga taataattaa
     5401 acttgccagc gtaacatatt tgcctaacgg caagcacagt atcgcaatgc cgactaagat
     5461 caaggctgaa ccaagtttaa tcatttgtct gttgctaaat ttaagcgtca agaagccact
     5521 tacgcctctg ccaacggtaa tgccgataaa gaaaatactg ccgaacgcag ccgcttgttc
     5581 taagccgaca tctttaacca ttacaaaata gctactcgcc cacagtccgg cggtttgttc
     5641 tacgccacaa tagccaaaaa aagctaaaat gccatatttc acgcccggaa tttttagcgt
     5701 ttcttttaag ctcatcggtt ttgccgaatg gttgatttca gcggtaatct gcttgcgtaa
     5761 tttccaaaaa ggcagtgagc aaaacagcat tagcgttagc ccgatttgta acattgaaat
     5821 ggtgaaatag cctgcctccc agcctcgccc ttgcaataag gcgtagccta aaatgtgcgg
     5881 cccgatagaa gctccaacgc cccacataca atgcagccag ctcatatggc ggcttgcgta
     5941 gtgaactgct acatagttat tcaacgccgc atcaaccgct ccagccccta agccgtaagg
     6001 aattgcccat aaacataaga tccagaaagc atcactgatt gaaaagcccc acaatgctaa
     6061 agccgttaag gcaacgctga tgaccattac tttgcctgtg ccgagatggt aggtcagttt
     6121 gtccgacatt aagctagagg taatagtgcc aatcgacata atcatcgaga ccacaccggc
     6181 ataggaaagc ggaacagcaa gctgttggtg cattgacggc cacgccgagc ctaaaattgc
     6241 atcaggcaac ccaaggctga taaaagagag ataaataata gcgagtagga ggttagtcat
     6301 agcttacaat cggtcacaaa aagcatcgct ttttgaacgt tggctctgcc aacgggagcg
     6361 aagctgtgaa caatccttac aaggattgtg aataattttt gataaatttt ttgtaaaaaa
     6421 atggcttaag gagaccttaa gccaaatttt attatttcac ttctgccaaa taggccttat
     6481 tagcacggtc aaaggtgtct aatacggctt tgcttggcat tggcgtgata agtgatacca
     6541 caatgatcat tagggtagca gcaataaagc ccgggatcat cgagtaaatt tccggtaagg
     6601 cgagcttaag tgtaatatcg ccccaaaaat gcacgattaa cgcaccggtc agcattccgg
     6661 caatcgcccc aaaaccgttc atacgtttcc agaatagtga aagcaatacc acaggcccaa
     6721 atgccgagcc gaaacctgcc cacgcattac gcactaagcc aaatactttg ctgtccgggt
     6781 cttgggcaat ataaatagct aatgctgcca ccgcaagcac catcaagcgg cttaaccaaa
     6841 ccagttcttt atcggaggct tgagggcgta aaatgccttt gtataaatct tcggtaatcg
     6901 cacttgaaca aatcagtaat tgagcggaaa gggtactcat taccgcagca agaatggcag
     6961 ataataaaat gccggctacc caagggttaa ataacaattt agctaattca atgaaaattt
     7021 gttccggatt tttgctgata ttgccgactt gctccgggtt gccaaagaaa tatgccatac
     7081 caaaataacc aatgcctatt gcccccagta agcagataat catccaagtc atactgatac
     7141 ggcgtgcatt ttcaagagac ttcacacttt ctgccgccat aaaacgcacc acaatatgcg
     7201 gttgtccgaa ataacccagc ccccacgcca ttaagctgat aatgcctaat acgctggtgc
     7261 cggtgaaaat gtcgttgtag tctttttgtg ccatctcgcc tgcacggtcg attaaggctt
     7321 gcatttcatc aaaaccacct aagttataca tcacaaaaaa cggggtgaga atcagggcaa
     7381 aaaccattaa tgaagcttga atagtatccg tccaacttac tgctaagaaa ccaccaataa
     7441 aggtgtaaag aatggtagca acagccccat accaaattgc ttggtggtaa ggtaagccaa
     7501 ataagttttc aaataccctc gcaccggcca caacgcctga tgcacagtaa attgcaaaaa
     7561 agagtaaaaa aatgaccgct gataaaatct taattaaacg ggttttatcg ccaaaacggt
     7621 tatgaaaata ttcgggtaag gttaaggcat cgccactgtg ttctgtatgc gagcgtaatc
     7681 tgcctgcgac taatttccag tttagataag caccgatagt taaaccgatt gcgatccagc
     7741 cttcgatcaa accgcctgca taaacggctc cgggtaatcc catcagcaac cagccggaca
     7801 tatctgatgc accggcagaa agtgcggtca caaagctacc taaacggcga ccgcctaaaa
     7861 tatagtctga aaggttattg gtaacacgat aggcaataaa gccgatggca atcattccta
     7921 gaatatagac aacaaacata attgttgttg gatcaagacc aaacattatt aatgaatcct
     7981 caagatttct tattgtataa agattaaagt gagtaaaggg taaaagatta cctgaaataa
     8041 tcaaaatggc aacacaaaat taccgtccgc ccagctcagc tgaaattttt tctgctgttt
     8101 cttgaatagc cggtagtaga ttttcccgcc ccagctgatt taaacgagca gttgaaagcg
     8161 aaacagaaat cgcataatgg actttttgtt ggtgatcaaa aatcgggcaa gcaatacaag
     8221 aaacccctaa ttcattttct tcgttatcca ttgcaaaacc ttgacggcga atggtttcca
     8281 gctcggtttg cattccgtca atcgtggtga tggtattgac cgtcagtttt tgaatgatgt
     8341 ccgatttagt ctgccaatat tgtggaatgt aatctgccga accataagca agaaacagtt
     8401 ttcccattcc cgagcaatag agttgcattt gttgcccgat ataggctcgg gtacgcatca
     8461 ttccggtggt agcttctaat ttattgatta aaatagcgtg atcgttttcc cgcatcgcaa
     8521 aatttaccgt ctcgcctaag gttaaattta gggcttctaa atgtggggcg gcaatatgca
     8581 aaatgttaat cgaacttaag gctcgctgcc cgattgccaa acatttggtg gttaaacgat
     8641 aactgcccgc agtcggtgca gggcggacgt agccctcttg ttgtaagcct tgtagcagac
     8701 ggtgaaccgt gcttttgttc agccccgaaa gctcggctaa atgggcaagc ggacagccgt
     8761 ttgggaattt cgctaacaat tccaatagtg ttaagccacg aattaaactt tgatttccac
     8821 tcgattcctt ttctttcttt gcattcattt ggctgctccc aaaataatat gatctaacgg
     8881 catatcccaa ctctcaatcg gtaattgttc gacttgctga cattcgtgtg ccaaaccgac
     8941 actaatcaga tgaggtgctt gagcaagggt tctatcataa aagccacctc ccattcccag
     9001 ccgatttccg gctttatcac aagcaacaag tggggtaaaa atcatctcta attcttgcaa
     9061 cggaaggata tttctaacat ctaatatcgg ctcttgaatc cccaagcggt taaattttag
     9121 aatagttttg caattgtaat ttacaaacaa taattgaccg cttgtaaaag ggtgcaacag
     9181 cggcagatac agctttttgc cctgttgaag taattgttcc attaaaggaa taggagaaat
     9241 ttcaccgtta aaaggcaaat aaaaggcaat atgtgagact tggtaagatt caatcaatga
     9301 aagagcttga ggaataatgg attcggctgc ttgagtttgc tgaccggcag ttaaactttg
     9361 ccgtttaagc cgcattgatt gccgtaattg gctacgaaga tcggttaaag atgattgatt
     9421 catataaaga cccgagatgc cgctaccagt aatggtcctt gaaccagtcg gttcaaggga
     9481 gtcaaactta actgctttta ggcttcttac tcctaattaa attagggtaa gcttgctcac
     9541 cagcgttaga tgtccaactc aaagtttcaa attatcggct cagggacgta actcgtaacg
     9601 cacaccccag gcaagactta tagtaacgga atttgatgat ttttcctagt atttaatccc
     9661 acaaaatgca aattctcaat ctttttggct ctattcctaa tcaattcgat taaaaattac
     9721 ctaattggat tgaaatttgc caagcgaatt atctaactgc tcaatacaag ccaccaacac
     9781 atttttattt tctgcatttt ggcgttgggc ttgctccagt tcataattta aattcagtgc
     9841 cacaatcgct aacacttttt caagctgaat aatgccggac tgctctttca ataccaccac
     9901 acgctcttcc aaacgctgtg ctgaagccag taaatgctct tgctgttcaa cagggcattg
     9961 caagcgtaaa acctgcccaa aaatctgtag ttcgatatta tttgccgaca taaatacgaa
    10021 atcctcactt aatcgcaaaa tttttgctat attatgccat tctttagcaa aatcacaaat
    10081 aatcccatta tggctatcac agattcccaa caatttaaac aactggtatc acaattacaa
    10141 attgatgata caccggcaga gtttcacggt tttttatgcg gtttaattgc cggcggtatt
    10201 caagatgagt cttggaaaac cctcacctac caatttacca atgacggaca cgcattttcc
    10261 atcgagccgt taaacacttt aaccgatttt tatcagcaat taagtgaaag cttttcagaa
    10321 gccaacacgc tgtttagcct ttggttgcca caaaacgaag aagatggttt tgccttagct
    10381 gatggtatct ccgaatggac aaacagtttc ttacttggac ttggcgttgc tcaacctaaa
    10441 ttacaacaag aaaccgatga ggttggcgag gcaatcgatg atttagatga aatcgccaaa
    10501 ctaggctaca gtgacgatga taataacgag gaactactgg aggctggcga agaagtgttg
    10561 gaatatctgc gagttttagc cttattcttg cacagccatt ttgctttagg cgtgccgaaa
    10621 accgaagaaa agccaccatt acactaatga ataaatacaa gcggttcaat ttcatctcta
    10681 ttttgcaaat atattatgcg gggcgaaaaa tatttcgccc ctaccaaaat caaaggaaaa
    10741 accaatgtca gaattaaaaa acgatcgcta tttaaaagcg ttattgcgtg aacctgtgga
    10801 tatgaccccg gtctggatga tgcgtcaagc cgggcgttat ttgccggaat ataaagccac
    10861 ccgagcagaa gccggcgatt ttatgtcact ctgccgtaac gctgatttag cgtgtgaagt
    10921 caccttacaa ccgctacgcc gttatgaact cgatgcagct atcctatttt ccgatatttt
    10981 aactgtgcct gatgcaatgg gattaggttt aagttttggt gcaggtgaag gccctaaatt
    11041 tgcccgcccg gtggcaagcc tcaatgatgt caatcagcta ccgattcctg atccggaaga
    11101 cgagttgcaa tatgtaatga atgcggtgcg taccattcgc cgagaattga aaggcgaagt
    11161 gccactaatt ggtttttctg gcagcccttg gacactcgcc acctatatgg tggaaggtgg
    11221 ttcaagcaaa gcatttacca aaatcaaaaa aatgctttac gctgagcctc acattctaca
    11281 caaattgctg gacaagttag ctgacagtgt catcttatac ttaaatgccc aaatcaaagc
    11341 cggtgctcaa gcggtgatgg tattcgacac ttggggcggt gtattggctc acgaggatta
    11401 caaagccttc tcattacact atatgcacaa aattgtggac ggcttaattc gagaaaatga
    11461 aggacgtaaa gtgcctgtta ccctattcac caaaggcgga ggcttatggt tagaagcgat
    11521 tgcagaaacg ggctgcgatg ccgttgggct agattggaca gtcgatattg ccgatgctcg
    11581 ccgccgtatt ggcgacaaag ttgcgttgca aggcaatatg gatccaagcg tgctgtatgc
    11641 aagccctgaa cgaatcgaac aagaagtaaa aaacattctt gccggcttcg gcaacggcag
    11701 cggacacgtc tttaatcttg gacacggcat tcatttagat gtgccggtgg aaagcccgaa
    11761 agtgtttgtt gatgcgattc acaactactc gaagcagtat cataaataat ggataaaatg
    11821 ccctctctcc ttgtgggaga gggacagatt tttctttgtt cagaagaaaa atcagggaga
    11881 ggggcaagcg gtcattttta tacagaattt tgcaaaatcg ttcccttctc tctgaaaaat
    11941 aaagaaggaa atacaatgag aaacccgatt cataaacgca tggaacgttt cgagccttgg
    12001 caaaacacca catttatggc gtgcctatgc gaaagaatgt accccaacta tcagcttttt
    12061 tgtgaagtaa ccgaacagcc tgaaaaagct aaggtattcc aaaatatttt aaatttagtc
    12121 tgggaatatt taaccataaa aggggcaaaa attaatttcg ataaccaact ggaaaaatta
    12181 gaagaaatca ttcctgatgt gaacgaatat gcgttttttg gtgtcttacc tgcacaggaa
    12241 gcttgcgaag ccctttctga acttcttcac agtattatcg ctggttcaac ccttgaacaa
    12301 gcaatccgca tcagccaaat ttcattaggc acagttgcca gctatttgga aatgcaacaa
    12361 gataaagaac tgaacgagca agagctaaag aattctgaag aaattcaaga agaactggac
    12421 gtacagtggc aaatataccg cttgctaaac gaatgtgaaa aacgtgatct tgatctcatt
    12481 tttgaactta aagatgaaat cagaagcagc gggattagta atatcgggtt aaatattaac
    12541 caatagcatt gatatttgca aaatcttgct taataatact ttgagaaatc ttaactttga
    12601 attaagctat cgcaaggttt caataacttc tcagacgttt tacgtcattt attaaaaaca
    12661 catatcttag aggataaaac tatgaacaaa actgagttaa tcgatgctat cgccgctggt
    12721 gcaaacttaa ccaagaaaga tgctaaagca gcattagaag caactcttga tgcaatctct
    12781 gcaagcttaa aagcaggcga cgctgttcaa ttaatcggct tcggtacttt taaagtaaat
    12841 gagcgtaaag cacgtactgg tcgtaaccca aaaactggtg aagagatcaa aattgcagca
    12901 gctaaagtac ctgcattcgt t
//
LOCUS       DANLXQ010000066        12803 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.066, whole genome shotgun sequence.
ACCESSION   DANLXQ010000066 DANLXQ010000000
VERSION     DANLXQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12803
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.066"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(200..1333)
                     /locus_tag="PXD94_001428"
     CDS             complement(200..1333)
                     /locus_tag="PXD94_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL6286290.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHGTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAA
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
     gene            complement(1350..2162)
                     /gene="dapB"
                     /locus_tag="PXD94_001429"
     CDS             complement(1350..2162)
                     /gene="dapB"
                     /locus_tag="PXD94_001429"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL6286291.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DAGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKRDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
     gene            complement(2213..2590)
                     /locus_tag="PXD94_001430"
     CDS             complement(2213..2590)
                     /locus_tag="PXD94_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286292.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTSQGFGNYTSEYILHYVKGEN
                     FIQIVDFTSHPPFKLPSESYLQN"
     gene            complement(2580..3053)
                     /locus_tag="PXD94_001431"
     CDS             complement(2580..3053)
                     /locus_tag="PXD94_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286293.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKSATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            complement(3121..4209)
                     /gene="rlmM"
                     /locus_tag="PXD94_001432"
     CDS             complement(3121..4209)
                     /gene="rlmM"
                     /locus_tag="PXD94_001432"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL6286294.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            complement(4209..4601)
                     /locus_tag="PXD94_001433"
     CDS             complement(4209..4601)
                     /locus_tag="PXD94_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL6286295.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIETGIK
                     YQMFHTLAILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYLLALNVP
                     TIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            complement(4708..6109)
                     /locus_tag="PXD94_001434"
                     /pseudo
     CDS             complement(4708..6109)
                     /locus_tag="PXD94_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(6264..8621)
                     /locus_tag="PXD94_001435"
     CDS             complement(6264..8621)
                     /locus_tag="PXD94_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL6286296.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSSGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQFADYVLGKADNLPKTAEWASQICDIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETMIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSGKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            complement(8757..11363)
                     /gene="acnB"
                     /locus_tag="PXD94_001436"
     CDS             complement(8757..11363)
                     /gene="acnB"
                     /locus_tag="PXD94_001436"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL6286297.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERADQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYAGKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQGLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            complement(11383..12630)
                     /gene="icd"
                     /locus_tag="PXD94_001437"
     CDS             complement(11383..12630)
                     /gene="icd"
                     /locus_tag="PXD94_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL6286298.1"
                     /translation="MQTQVQIPQGETIQLNQDGSLQVPDNPIIPFIEGDGIGVDVTPA
                     MKAVLDAAVEKAYQGKRKIAWMEIYAGEKANKLYGENTWLPDETLELIRQYHVAIKGP
                     LMTPVGGGIRSLNVAMRQGLDLYNCLRPIRYYDGTPSPVKHPELVDMVIFRENSEDIY
                     AGVEWVAGSPEANKVIEFLQQEMGVKKIRFTEDCGIGIKPVSKQGTQRLVRAALQYVI
                     DNNRKSLTLVHKGNIMKFTEGAFKEWGYQVAEEFGAKLIDKGPWMSLTNPKTGEEIII
                     KDSIADAFLQEILLHPTEYDVIATLNLNGDYISDALAAQVGGIGISPGANIGFEAAIF
                     EATHGTAPKIAGQNKGNPGSLILSGEMMLRHLGWVEAADLVVKAVSKTIANKTVTFDF
                     AEMLEGVTLRSTSEFAQDIIANM"
BASE COUNT         3651 a         2963 c         2448 g         3741 t
ORIGIN      
        1 tttaaaaatc tgttcgtttg cttgttttat aagcaaacta atttcgcata attattatta
       61 tgttaaatag atagttaaaa taacacattt aattaaatct tttcgtgttt ctctcctcct
      121 ttattaaaaa agaaaacggt cagattggta aaatctatac ctaatatgac cgcttgtgaa
      181 tacaaaaaaa ttacttcaat tacttcaact gtttaaaggc ttgcagtaaa tcagctttaa
      241 tatcttcaac gtgttctaaa ccaacggaaa aacgcagtaa attattcgta atgcctcgtg
      301 taatacgttc tgcttccgga atatccatat gggtttgtgt tgcaggataa gtaataaaac
      361 tttctgcacc gcctaaactt tccgcaaagg taatcaattt aattgccttg agaaatttcg
      421 ctacccactc ttctttttgt aaacggaaag ataacatacc acttttgcct gaatataaca
      481 cgctgtcaat ttcaggttgc tctgctaaga atttagccag ttcggtggca ttagactgat
      541 ggcgttccat tcgtaatgcc aaggttttca taccacgcac ggcaaggtaa gaatcaaatg
      601 gcgacagcac tgcaccggca cagttttgga aatagaataa acgctcggtc aaattctgcc
      661 cgtttacttt ggtagcatca gaatctttcg caacaattaa gccggctaac acatcattat
      721 gccccgaaag atacttggtc gcactgtgaa tcacaatatc agctccgtgc tcaatcggac
      781 ggaataacgc tggggttaag aacgtattat caacgatcaa cattaaattg tgttttttcg
      841 caattttagc aatggcagca acgtcgcact cctccatcaa tggatttgac ggcgtttcca
      901 cgaaaatcgc cttagtattc ggtgtaatag ctgcttcaat cgctgctaaa tccgctgtat
      961 taacataaac cggctttaat gtgccgtgat ttttgtggct aaaatcaaac aagcggtaag
     1021 aaccaccata aacatcgctg gaaataatcc attcatcagg tgctttaaat aatgacatta
     1081 ctagctgaat cgctgccatt ccagatgcca tcgcaaaacc ggcatcaccg ccttctaaat
     1141 ccgcaatccc ctgctctaat acagctcgag ttgggttttt ggtacgggtg taatcccagc
     1201 cggtagattc gcctaagcca tcacgcccgt aagcagttga taagataatc ggcattgcca
     1261 ccgcccctgt acgaggatca gttctgttac cgatttgcac taatgtggtt tcaatattat
     1321 tgaattttgt cattgttatt atccttcaat tacagattat ttagatccaa tacgtctgtc
     1381 atatcaaaca agccattttg tttgccattt aaccattttg cagcacgcac tgcgccattg
     1441 gcaaacgtca ttcgactaga ggctttatgt gcgatttcga cacgttcgcc ttcatcagcg
     1501 aaccatacac tatgctcccc tacaacatca ccggcacgaa tagtagcaaa gccgatttca
     1561 tcacgtttgc gttcgcctgt aataccgtta cgttcaaata cgccatgagt tttcaagtca
     1621 cgcccaagag ttttggcaat atgttctccc attgataaag ccgtacctga tggtgcatca
     1681 actttatggc gatggtgtgc ctcaatcact tcaatatcac aataatcgcc cattactttt
     1741 gctgcttttt cgagcagttt aaacaccaga ttcaccccaa cgctgtagtt tgaggcaaaa
     1801 acgataccga ttttttcact cgctgctttg attgcagctt tacctgcatc atcaaaaccg
     1861 gttgtgccaa tcaccatttg tttatgatga gccacacaaa tatccaaatg ttctagcgtg
     1921 ccttcagggc gagtaaaatc aattagtaaa tcaaaacctg ctacattttg ggcgacatca
     1981 tcagaaatca ccacacctaa tgtaccaacc ccggctactt caccagcatc actacctact
     2041 aatgatgaac ctttacgctc aaaagcagac cctaattcta cgccttgtgc attatggacg
     2101 gcttgaatta attgacgccc cattcgccca ccggcaccga cgattccaat ttttaatgtc
     2161 atatttgttc ttccttttta tatttgtttt ttacaagcgg ttacattttt aattaatttt
     2221 gcaaatagct ttctgatggc aatttaaaag gtgggtgaga agtaaaatca acaatttgaa
     2281 taaagttttc acctttcaca taatgtaaaa tatactcaga agtataatta ccaaatcctt
     2341 gactggtatc atattctgga atgccgatat gataatgcca taaagaatat ttttgtgctt
     2401 tagccacctt ctctgcccaa taaggatcat tcgttggcac atcatcagaa cttttatttc
     2461 gaccagctag attttccaat ccataaattt caacgtgttg tttaaactgc aggattttga
     2521 ctagatcttt ttcaggcaaa tattgcaatt cggctgaaat ttttgtacca aataaaactt
     2581 taatgcccat ttgcaacacc tttcaaccag tttctaaatt cattaacatt cgttatatct
     2641 ttaggcactt cgtgaaaagt atcatttaac cgctctttca ttcgctctaa gtcaaaatta
     2701 aatgcggttg cttgagagag ttgctgttca attttgcgaa tattggactt catattatcc
     2761 aatatagcca ttgtccttga gccatcagta aaatactgtt gggtatcgaa aatcaccacc
     2821 ttattttgat ttaggtattt tttgaaatcg tttaaatcaa tttcagccca aaagtttgga
     2881 tttttttcga tagtttcaat acaggcaatt aataattgat ttgccattgt aatacgaaaa
     2941 tttaacaatg agatttgctg ctctgttgca gatttatcat cagctgcgaa atcaaatgcc
     3001 ggataaatca ctgcaagcgt gtcagccata gataagggta aatttttcat catctctcct
     3061 tacggaagaa gttgtcatta tctactggat tacttaaaaa aacaacttat tcattttatg
     3121 ctatttttgc cccatcaccg caatatgtac cgtaatttcc tcacggtcgt ggtagaggtg
     3181 tttagcttgc attttgaacc aaaacccgtg tttcgttagc tcttcttcca agaaattgag
     3241 gcatagttgc acttcttgat aacgtttttt catcggcagt tttaggttga aaatcgtttc
     3301 tcgacaccaa ccgttagtca gccattttgc aatgagtttc gagatacgaa tcggctgctc
     3361 taccatatca caaaccagcc aatcgatttg tttacgctta ggcggttgga atttaaaccc
     3421 atcttcaggg caatgctcaa ttcttccggt ttcgtgtaaa cttgctgcca ttttgccgtg
     3481 atcgactgca tagacaaaaa caccacgttt gactaattga taagtccaac cacccggaca
     3541 agccccaaga tcgaccccta ccatcgtatc attaaagcgt tttttctcct ctgcttgcgg
     3601 aataaaagtt aaaatcgcct cttctaattt aagcgttgag cgactaggtg cctcagccgg
     3661 gaattttaaa cggggaatcc ccataaaaaa cggcgattta ttatggttgt acgcatagcc
     3721 cacatagcaa gctcccggac gagtaaataa aatatgcaag ctcacactac cctgcatatt
     3781 ttccgatttc cccaaccaac cttgtttttt caatgccgac cgtaatggca cagtaaattt
     3841 acgacagaaa gttaataatt ctttcgcttc gttggtatcc ggtgtttcga caaatacatc
     3901 agaactttgg cgaggattaa gttcggcata ttgggctaaa atcggcgaaa ttcgatcatt
     3961 ttcagacaga ttttgcaaca aatccccaac aaccaacatt tggcgaacaa aaataagctg
     4021 ttcaaacttt aacgcttggg ctaatcgatc tgcttcccct gcttgatagc actcaaaaat
     4081 cacatagcca ctattttctt taaggttggc aaagccaaaa atgccctgtt gtgcagcttt
     4141 atccgtgatt tcgcctgcta cctctttttc aaaccctgct cggcaatata aggctaattt
     4201 attgaacatt aggcttttct cacactgata taaattaacg caatccaacc aagcataaag
     4261 cagagaccac caataggcgt aagccaatga atagtcggca catttaacgc cagtaagtac
     4321 aggttgccac taaaaagcag aatgcctaac gcccaattgc ttccgatgat gttaaaggct
     4381 ttggctctac aaacgggtgg attttgccca aaatttgcaa tttggaataa acctaaagct
     4441 aaaatggcaa gggtgtgaaa catttgatat ttaatccccg tctcaatcca gcctaaggct
     4501 ttaggcgata attgagccga taatgcgtgg ctggctatcg ccccaaatgc cacagtaaat
     4561 aagccgctga atgcggcaat tgctacaaat ttgtttttca taagttgaag ataggtatga
     4621 tgttagatgg cttactatat catattatcg aaaccaacaa gcggtgaaat tttgcaaaaa
     4681 aattacaaaa aatcaccgtt tgtatgctta atggttcaag ataatatcca catcttccga
     4741 tttatcgtga accccaaatc tatctactaa ggttttgctg gcctcattta atccgataat
     4801 ttctacctca acatcacttt ttcgatattt caacacaact ttatctaacg ctgaaactgc
     4861 cgttacgtcc caaaagtgag cgtgggttaa atcaatgtgg acttttttca gttctgaacg
     4921 ggtaaaatca aattgtgcga taaaatcatc agatgaggca aaaaatactt gcccgataac
     4981 atcataacgc aattcctctt tctctatagc acttgtttta accgccatat aacgtccaat
     5041 tttattcgca aagaataatg ccgagagcaa cacgccggaa aatacaccaa gggctaagtt
     5101 atgtgtataa accacaattg caacagtgac taacatcact acattaaagc cgattgggtg
     5161 cgtttttagc tcttttaagg aatgccaatt aaaggtgccg atagagacca taatcatcac
     5221 tgcaactaat gcagccatcg gaatcactct caaaatatcg ctcaaaaaga ccacaaaaat
     5281 cagtaataat attggcaatg ccctgccctt tacattcacg gtttttatcg ctgtctgtct
     5341 ctgtcatttc atcaacaatc gttgccgtca ttaaagattc caataaccct actgcggcca
     5401 agctgaaaga ataaggcaaa ataatccata aggtgtctaa attaagcgga atatcgggta
     5461 ataaaaacac cggtaaggta tcgggtaatt gccccatatc cccaacggta cgaacatcaa
     5521 tgccttgcca taatgtgaaa agtgttaaag caataatggt cactaatggt gagggaatga
     5581 tttttccaat caccggtaca taaggaaaaa gataaatgat agcaagccct gctaccacca
     5641 gtgcataagt atgccaagtt gtattttgta actcaggtag ttgtgccata aaaattaaaa
     5701 tggcaagtgc attaacaaac ccaatcacta cggctcgtgc cacaaaccgc ataagcgagc
     5761 cgagttttaa ccaaccggca ataatttgca acacgcctgt taataaggtg gccgctagca
     5821 tatattgtaa accgtgatcc ctcactaagc ctgctaatac cagagccatt gcaccggttg
     5881 ctgcagaaat catcgccggt ctgccgccca caaaactgat aattactgca atacaaaatg
     5941 aggcatataa accaatttgt ggatcaaccc ctgcaataat tgaaaatgct atggcttctg
     6001 gaattaacgc caaagccacc actaaaccag agagaacatc acctcgtaca tttgaaaacc
     6061 attgttgttt aattaccgta ggcgaaaatt gctgaaataa agttgtcatt attaccctta
     6121 aataaaaaga aaagccgagc aaagtcggct aaaaatgagg cgagagagta gcaaattagt
     6181 gaaaagtcgt caaataaaca agcggtcttc tttcgctctt tttttgcaaa aaatagtaga
     6241 aaaatgaccg cttggattaa ttgctattca ctaaaaaaag gggctttaaa caccgtcact
     6301 tcaggtgcat ctcccacata tttttccact tgaaccgtgg tagtgttcgg tgcattgccc
     6361 tgcccgagtt ttgatgaacc ctcattacgg gttagcacat tcgggttgcc gtttttgcat
     6421 agtggagtat ctgttttgcc taaatcttgc gggtcatacc aaccaccttc gtataacgct
     6481 actgtgcctt gaatgatgcc atcagtcacc actgctcctg ccaacacctg ccctcgcttg
     6541 ttaaaaattc gcacaatatc accgcttgta atgcctctgt cagcggcatc ttttgtgtga
     6601 attagcattg gttctctgcc gtttacctcg taatattgac gtaatgagga gtaagcaagc
     6661 tggctgtgta aacggtattt aatatgcggt gtaactaatg ccaacggctc ggcttcgttg
     6721 gttttgcctg aatattcgtg atgttcaaac caagtcgggt gtcctttaca atcttcataa
     6781 ttcatcttgg caatggtttc ggagaaaatc tcaattttgc ctgatggcgt tgccaatttg
     6841 tgtttaatcg gatcagtacg ataatcttga tagcgaacga aagtattatt ttgatgttct
     6901 gcctcaaagc tcaacacttt attttccgcc caaaatgcct cgaaatccag caatggcgta
     6961 ttagcttttt cccccgcctc aaaggcaact tggtaaaatt ccttaagcca ttccatttcg
     7021 cttttgcctt cggtaaatgc ttgttcttta ccggctcgtt tcgctaattc ggcaaaaata
     7081 tcgtaatcat tcttcgcctc aaattgcggt tcaaccacct gtttcatcgg gataatatgt
     7141 tttgctacat aatcaccggc aacggtgaga tcgtttcgct cgtagctggt ggttgcgggc
     7201 aatacaatat ctgccattcg agcggtcggt gtccagtaac attcattgac gatcatcgtt
     7261 tccggctgtt gccacgcttt cactaaatag ttggtatccg ggtggtgagc aaacggatta
     7321 ccccccgccc aatagactaa tttgatgttc gggtaatgta atgtttgccc gttgtaatca
     7381 taaggctgat tcggtttgag caacgcatcg gcaattcgag acaccgggaa aaacggcata
     7441 tcggggttag gtttacctgc attgattgcc cctaaacgaa caccggaatt aacatttagc
     7501 ccattggtag aataacgata atttaagccg aaaccgccgc ccggcaagcc aatttgcccg
     7561 agcatagcgg caagagtcac taacgcccag tggctttgct ccccgtgttg caggcgttgc
     7621 aagccccagc cggacatcaa cattgtgcgg tgagtaacaa aatcatttgc taaggtttta
     7681 atcacctcct ccggaatgtc acaaatttgg ctcgcccact ctgctgtttt aggtaaatta
     7741 tctgcttttc ctaacacata atcagcaaat tgctcgaaac caacagtata tttagttaag
     7801 aagtcgtgat cgtgcttgtt ttcagtaata agggtgtggg cgatccctaa aatcagtgca
     7861 acatccgtgc cggtattaat aggcaaccac tctgcctcta aaaattgaca ggtctcgctt
     7921 ttcaccgggt cgatacaaat aatccgttta ccgcttgctt ttaatttggc gagataatca
     7981 atcccctccg gttcggcaga ggtccacgca atgcgaagtg tattcagtgg attagacgac
     8041 caaattacca ataattctgt gctttctaca attgtgtgcc aactggtttg ttgagcatac
     8101 acctcaatat tgcctaatac ataaggcata atcacttgca ccgcaccggt ggaataatcg
     8161 cctttagtat tggtaaagcc gcctgtgacg tttaaataac gttgcaaaag cgtgcgagag
     8221 gcgtgcagtg aaccactgct ataccagccg taagaaccgc cgaaaatgga ttcgctgcca
     8281 tattgctctc ttacccgttg gatttcgccg gaaaccagct caaaggcttt gtcccaagag
     8341 attctcaccc acttatctcg ccctcgcaaa gtggtgtctt tattcccttc caagtagcct
     8401 ttccgcacca tcggatattt cacccgaacc gggctataga gctgatccgc caccacatct
     8461 tgcaattcgt tttggtgatt tttcgctaaa ctgccctctg atttcaccac ttttccgttt
     8521 tccaccgtta catttaatgc accccagtgg gcggcggtgg taatggtatt tttcggttta
     8581 tccaaaaaga caaacttccc tttttctaat gctgtgttca tttaaattaa tgccttttct
     8641 gttacaaaat tgagaggaat ttactggatt aagccaaaag ttgcaaccac aagcggttgt
     8701 ttttagccaa aaatttgcaa aacaaaaccc acctaaaaag gtgggcttca aggaagttaa
     8761 acggcaactt gaacgattac attgtccgct tttttcacat aactattgat ttggtcaaag
     8821 ttcatatagc ggtaaatatc gtccttatct ttctgcaagt tattcatata gtctaggtac
     8881 tcttgcggtg tcggcaattt acccaataac gcagaaactg cggctaattc tgctgaggct
     8941 aaatacacat ttgccccttg ccctaaacgg ttcgggaagt ttcgggtaga ggtagaaacc
     9001 acattagcat tatttgccac tcgggcttgg ttacccatac agagagaaca gcccggcatc
     9061 tctacccttg ctccgctttt gccgtaaatg ctgtaatagc cctcttcggt cagcaagcct
     9121 gcgtccattt tagtcggcgg agcaatccat aatttagtcg gcaattcgcc ctgtgcttgg
     9181 cttaacagtt tacctgccgc acggaaatgc ccgatattag tcatacaaga accaataaag
     9241 acttcatcaa ttttatcgcc ctgtacttga gaaagcaggc gagcatcgtc agggtcattc
     9301 ggtacgcaca caatcggctc tttgatttca tctaagttga tctcaatcac tgcggcatac
     9361 tccgcatttt cgtctgcctc taaaagctcc ggattatcca accacgcttg catattggcg
     9421 atacggcgtt ctagcgtgcg tttatcgtca tagccttccg caatcatcca ttttaaaagc
     9481 gtaatattag aattcagata ctcgataatc ggctctttgt tgagcttgat ggtacaggct
     9541 gcagcagagc gttcagcaga agcatcagaa agctcgaagg cttgttcgat tttcagatct
     9601 tccaaccctt caatttctaa aattcgaccg gagaaaatat ttttcttacc ttttttctcc
     9661 accgtcagca agccttgctg aatggcataa tacggaatag cgtgaaccag atcccgtaag
     9721 gtaatgcccg gttgcattgt gccggaaaaa cgcaccaaga ccgattccgg catatctaac
     9781 ggcatcaccc ctgttgccgc agcaaaggca actaagcccg aaccggcagg gaatgaaatc
     9841 ccgattggga aacgggtgtg ggaatcgccg cctgtaccga cagtatccgg caataacata
     9901 cggtttagcc aagagtgaat cacgccatca cccgggcgaa gtgacacacc gccacggttc
     9961 ataataaaat caggcaaagt gtggtgggta attacgtcca ccggtttcgg gtaagcggca
    10021 gtatggcaga acgattgcat cacaagatct gccgagaagc ctaaacaggc taaatctttc
    10081 agctcatctc ttgtcattgg tcctgtggta tcttgtgaac cgacagacgt cattcgaggc
    10141 tcgcaatatt gccccggtcg aacgccttct acgccacaag ctcgccctac cattttttga
    10201 gcaagggtgt agcctttgtt actctctgaa accgactgcg gtttaataaa tacatcactt
    10261 tcaggcaagc caagtgctac tctggcttta tgggtcaaac cacgtccgat aatcagcgga
    10321 attcgcccgc ctgcacgcac ttcatcaagc aatacttcgg tttttagctc aaattcggct
    10381 aagacttcat cggagttgtg tttgcaaatt ttgcctgcat aagggtaaat gtcgattaca
    10441 tcgcccatat tcaggttggt tacatccact tcgatcggta aagaaccggc atcttctaat
    10501 gtgttaaaga aaatcggggc aattttaccg cctaacacca ccccaccggc acgtttgttt
    10561 ggaatgtaag gaatatcttc ccccataaac caaagcacgg agttggttgc ggatttacga
    10621 gaagagccgg tgccgactac atcaccgaca taggctaacg ggaaaccttt ttcttttaat
    10681 gcttccagtt gcttaatcgg gccggtatga ccgttttcat cggggaaaat accgtcacgt
    10741 tcatttttca gcattgctaa tgcgtgaagt ggaatatcag ggcgagacca agcgtcttga
    10801 gccggggaaa gatcatcggt attggtttcg cctgtgactt taaataccgt taccgttaat
    10861 ttttcggcaa gtttcgggcg agaggtaaac caatcggcat tcgcccaaga ttccaatact
    10921 tgttgtgcaa acggattacc ctgatctgcc cgttctttta catcgtggaa attatcaaac
    10981 atcaataatg ttttagataa ggcttctacc gcaatcggtg caagctcagg gttatccaac
    11041 gcagacaata aggtttcaat gttataacca ccttgcattg tgccgagtaa tttcactgcg
    11101 tgagcaggcg aaatgagcgg cgatgtggca acccctttgg taatatcggc taaaaaggcg
    11161 gctttaacat aagcggcttc atcaacacct gccggaatac gattggtaaa aagatccaat
    11221 aaaaagaccg cttgttcagc cggtgggttt ttcaataatt caaccagttg agccgtttgt
    11281 tccgcagaaa gtggctgagg cacaacaccc agtgccgcac gttcatcaac ctgttgttga
    11341 taattttcta taaaattttc catcatcatt ccctctccaa ctttacatat tggcaataat
    11401 gtcttgtgca aattctgagg ttgagcgtag agttaccccc tccagcattt ccgcaaaatc
    11461 aaaggtgaca gttttattgg caatggtttt agacaccgct tttaccacta aatcagccgc
    11521 ttcaacccaa cctaaatggc gtaacatcat ttccccactt aaaattaatg acccaggatt
    11581 gcctttattt tgccctgcaa ttttaggggc tgtgccgtgt gtggcttcaa aaatagccgc
    11641 ttcaaaccca atattcgctc ccggtgaaat accgattccg cctacttgtg cggctaaagc
    11701 atcagaaata tagtcgccgt ttaggttcag tgtagcaatc acatcatatt cggttgggtg
    11761 taacaagatt tcttgtaaaa aggcatcggc aatgctgtct tttataatga tttcctcacc
    11821 ggttttcgga ttagtcaagc tcatccaagg gcctttatca attaattttg ccccaaattc
    11881 ttccgcaact tggtagcccc actctttaaa tgctccttcg gtaaatttca taatgttacc
    11941 tttatgcaca agcgtgagcg atttacggtt gttatcaatt acatattgca atgcagcacg
    12001 caccaagcgt tgtgtgcctt gcttagaaac cggcttaatt ccgataccgc aatcttcggt
    12061 aaagcggatt tttttcacgc ccatctcttg ttgtaaaaat tcgatcactt tattggcttc
    12121 aggcgaaccg gcaacccatt ctacaccggc ataaatatct tccgagtttt cacggaaaat
    12181 caccatatcc accagctcag ggtgtttaac cgggcttggc gtaccgtcat aataacgaat
    12241 tgggcgtaag cagttgtata aatctaatcc ttgacgcatc gccacattta aagaacgaat
    12301 accgccaccc accggtgtca ttaaaggacc tttaatcgcc acgtgatatt ggcggataag
    12361 ctctaaggtt tcatccggca accaagtgtt ctcaccatat aatttattcg ccttttcgcc
    12421 tgcgtaaatt tccatccaag caatttttcg cttgccttga taggcttttt ctaccgccgc
    12481 atctagcact gctttcattg caggagtaac atcaacaccg atgccgtcac cttcaataaa
    12541 tggaataatc ggattatccg gaacttgtaa agaaccgtct tggttcagtt gaatagtttc
    12601 gccttgtgga atttggactt gtgtttgcat tgattttacc cccgtaatga cgaaaaagta
    12661 gtgacgaaaa atgaatgttg acaatcagcc aacaatggat taactataaa cagtaaattt
    12721 ctaaatgtaa ataaagcaaa atgggctgag agagaatttg tgatctaacg cacatttatg
    12781 taaataaaat gtaaaaaatc gtt
//
LOCUS       DANLXQ010000067        12715 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.067, whole genome shotgun sequence.
ACCESSION   DANLXQ010000067 DANLXQ010000000
VERSION     DANLXQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12715
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.067"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            6..1088
                     /gene="serC"
                     /locus_tag="PXD94_001438"
     CDS             6..1088
                     /gene="serC"
                     /locus_tag="PXD94_001438"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL6286299.1"
                     /translation="MTEIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGK
                     LFMELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHW
                     SATAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYIHYCTNETISGVEIFD
                     IPNVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARK
                     DTPSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKATLLYE
                     YLDSSTFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRA
                     SIYNAMPIDGVKALIEFMKVFEQRNA"
     gene            complement(1145..2122)
                     /locus_tag="PXD94_001439"
     CDS             complement(1145..2122)
                     /locus_tag="PXD94_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL6286300.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQVSAEVFSRDVL
                     MMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAG"
     gene            2234..4801
                     /gene="glnD"
                     /locus_tag="PXD94_001440"
     CDS             2234..4801
                     /gene="glnD"
                     /locus_tag="PXD94_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL6286301.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
     gene            complement(4978..5736)
                     /locus_tag="PXD94_001441"
     CDS             complement(4978..5736)
                     /locus_tag="PXD94_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL6286302.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGAGKSTLL
                     SLIARLQPIQQGEIHLNELNIANTASQTIARQLAILTQDNVIHSRITVKDLLLFGRYP
                     HHQGRITSHDNAIVEEALVRFDLSTFSHRFLNELSGGQRQRALIAMTFCQQTDHILLD
                     EPLNNLDMFHARELMKLLRKLTDELALTTIMVVHDINIAAAYADTIIAMKDGGIKMIG
                     TPKEIITTENLKTVFNLDAEVLECQGKPLVIHHI"
     gene            complement(5756..6632)
                     /locus_tag="PXD94_001442"
                     /pseudo
     CDS             complement(5756..6632)
                     /locus_tag="PXD94_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
     gene            complement(6685..7653)
                     /locus_tag="PXD94_001443"
     CDS             complement(6685..7653)
                     /locus_tag="PXD94_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL6286303.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKEKRAEEKARG"
     gene            complement(7747..8646)
                     /locus_tag="PXD94_001444"
     CDS             complement(7747..8646)
                     /locus_tag="PXD94_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6286304.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTARGEVTFKETPSKIAV
                     FDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFEPDLEALNSLNPDLII
                     VGTRTAKKFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFGKLFNKEAETEKLKAEINT
                     LLDQTKAAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNLGIPMADSSIKNTGHGQP
                     VSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQTKAWKNGNIVYLSAASY
                     LAPGGYEQMKTDLTNIKTAFEKK"
     gene            8820..10283
                     /locus_tag="PXD94_001445"
     CDS             8820..10283
                     /locus_tag="PXD94_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL6286305.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDTLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            10273..10578
                     /locus_tag="PXD94_001446"
     CDS             10273..10578
                     /locus_tag="PXD94_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL6286306.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            10578..11027
                     /gene="nrdR"
                     /locus_tag="PXD94_001447"
     CDS             10578..11027
                     /gene="nrdR"
                     /locus_tag="PXD94_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL6286307.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIYLSFSDIEEFIKEIEKLKE"
     gene            complement(11101..11283)
                     /locus_tag="PXD94_001448"
     CDS             complement(11101..11283)
                     /locus_tag="PXD94_001448"
                     /inference="COORDINATES: protein motif:HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6286308.1"
                     /translation="MALLADSTVDRLVAENGLVEVLGETENPKFDLTMIYAAHRYPSS
                     IVRAFVGFVLEQVKAN"
     gene            complement(11252..11962)
                     /locus_tag="PXD94_001449"
     CDS             complement(11252..11962)
                     /locus_tag="PXD94_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6286309.1"
                     /translation="MNLNTLRLFVAVIQHGSLSKASERLNVPIATISRQIADLEKELN
                     IQLFDRQKSGVKPTMAGQRLYEQVHLSIEQLATAKEVLFEEQGNLKGKLRLSASPACE
                     PVLAWIADFQAAYPQVQVHCTLTDRLLDLSADGIDVAFRIGQLHGEQFIAKKVGQIGS
                     KWVAHPKFLARFGTPSTVKDLKNFPIAAWAKNEASVITLGTGRQAVEIPYLFSSNDSY
                     AIEYMAKSSGSACRQHGG"
     gene            complement(11959..>12715)
                     /locus_tag="PXD94_001450"
     CDS             complement(11959..>12715)
                     /locus_tag="PXD94_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286310.1"
                     /translation="WVKINDFPDVSNLLEVTINDEQWLVLERHDDFRELAKFGQKETY
                     DRQMWLQIRSYLVKESQFSKAKNWLAQQDFMERWMPEGNEHYGMNIHQFFAENTLNEW
                     QEIYRHRENKSPSFKVIPSVDSHTWESTYGNDGISFYAPIPMLYHGMKMKNAIKDGYW
                     VDLQNNLVCFNPEINEKFQNSLLVKKDKFLDFLKEHKLKLVWTVLGEKHAYKESKFLQ
                     ELSGVYYLYGKNQQVQGSIKHFIQEIEHWGEKQ"
BASE COUNT         4068 a         2521 c         2555 g         3571 t
ORIGIN      
        1 gaaaaatgac agagatatat aacttcagtg ccggcccggc aatgatgccg aaaaaagtac
       61 tcgaaaaagc gcaacaagaa ttattaaatt ggctagacca aggcacatca gtgatggaag
      121 tcagccaccg tggaaagcta tttatggagc tggcggcaca atctgaagca gatttacgca
      181 agctgtataa tgtgccggac aattatcgta ttctatttct tcaaggtggt gcaagggggc
      241 aatttgcagc cattccaatg aatttaatcg gtaaaaaagg caaagcgctt taccttaatt
      301 cagggcattg gtcggcaaca gcagcaaaag aagcacgtaa ctttgcggaa attgatgaaa
      361 tcaatatttt agagactgaa ccgcaactta aagtgagtcg tttagatttc agcgatattg
      421 cagagcaata tgattatatc cattattgca ccaatgaaac catcagtggg gtagaaattt
      481 tcgatattcc gaatgtcgga aataccccgt tagtggcaga tatgtcgtct aatatcttag
      541 cccgagggat tgatatcaac aaatttggag taatttatgc cggagcgcaa aaaaatctag
      601 ggcctgctgg cattacgatt gtgattattc gtgatgattt aattggcaat gcacgcaaag
      661 ataccccatc tatttggaat tatgcaactc agcgagatgc tgattcgatg attaataccc
      721 cgccaacttt tgcttggtat ttatgttctt tagtgttcca acatttactg gcagagggtg
      781 gtttgaaagc tactgaagaa cgtaatcttg ctaaagctac attgctttat gagtatttgg
      841 atagttcaac attctattac aatacggtag cacacgaaaa ccgttcctta atgaatgtaa
      901 cctttaccac gggtaatgac gagctaaatg caaaatttgt ggcagaagcg accgcttgtg
      961 gtttacaggc attgaaaggc cataaagtat taggtggaat gcgtgcctca atttataacg
     1021 caatgccgat tgatggggta aaagcactca ttgaatttat gaaggtattt gaacaacgta
     1081 atgcttaatt caagcttaag aatataaatt aaagagccac gtttgtggct ctttgaattt
     1141 ggcactagcc cgcagtttta aacaaataga aattatagcc tacataagcg gcaaagagta
     1201 ataagccctc taaccgatta attctgccac cttctgtccg acgatagagg ttgatcccga
     1261 aaatgaataa taagaaggtg agagccgaca tcatcaagac atcgcgagaa aacacttccg
     1321 ctgaaacttg cattggttct atagcaccgg caatccccac cacggctaac gtattaaata
     1381 agttagaacc gataatgtta cctaaagcta aatccacttc tcctttacga gcagctgcaa
     1441 tagaagaggc taattcaggt agtgaggtgc ctaccgccac aatagtaagc ccgattacca
     1501 aatcactcac accaaaaaat ttagccactt caactgctcc ccagactaag aattgggaac
     1561 taattactag cgatactaaa ccaataatta gccaaagaat ggattttcct aatcccatct
     1621 tagcactatt ggttaattct gcatcaacat ctaagcttaa tgcatcattt ttacttttca
     1681 ttgcggtaat aattgtccac gccatatata caaaaaacac agcaagtaaa ataatcgcat
     1741 caagttgagt gacgtttgca tcataaacca aataagcaga aagcagggtg acagccagta
     1801 aaatcggcaa ctcttttttt aacacatcgg aattaactga tagtggttta attattgcgg
     1861 ttaaacctaa aatcagcgca atattggtaa tgttagaacc gtaggcatta ccgagagcga
     1921 tacccggcga tccgcttaat gcagaagatg cagatacgag catctccggt gcagatgtac
     1981 caaaaccaat gaccacaata ccgattaaca atggtgtcat accaaaatgg cgagccgtag
     2041 aggctgcccc ttccacaaaa cgatccgcac tccaaactaa tataattaag ccaccgataa
     2101 tagcggcaac agcaagtgtc ataaactatc cttttagatg aaaaacataa aggcattata
     2161 agcattttag ccaaaataaa tataggcaaa cgaaaataaa tgacctatta ttttgccagt
     2221 ttaggaagag attatgatga aaaacagcaa cgaattaaaa acgcttttat cagcatttac
     2281 ggagcaacag cggcaagcat ttcaatccga aaatgtactc agcttacttg cacaacgtag
     2341 ccaattttat gaccaactac tgcaagcact ttggcaagat tttggactcg ctcaatatga
     2401 acatattacc ctattagcgg taggggggta cggacgtgag gagatgtttc cgctttcgga
     2461 tctggatatc ttagtgttaa ctgaaaaacc gctagaagag gctattcagc agcgtttaaa
     2521 cgaactattt gccttgcttt gggacagcaa actgcaactt ggcaccagtg tgcgtacgct
     2581 cgaagaatgt attcagattg ggaaagcaga aatttcggtt gccactaata tgttggaagg
     2641 gcgattttta ttgggtaata aatcgctttg gctaagatta aaagaagcac tttatcagcc
     2701 ggatttctgg gatattaaag cgttttttag agcgaaaatg gcggaaaaaa atgagcgtta
     2761 tgcacgttat cataatacta gctataacct tgagccagat ctcaaacata gcccgggagg
     2821 attgcgagat ctgcatttaa tctcttggat tatgctacgg cattatggtg tatcttcttt
     2881 tgtcgatctc ctcaataaag ggctacttta cccggaagaa tataaggagc tgatcgcagc
     2941 acaagcggta ctttttcgga tgcgttttgc acttcatttg caactgaaac gttacgataa
     3001 tcgccttcgg tttgacagac aattacaact gagtgaaata ttaggctacc aaggcgaagc
     3061 aaatcaagcg gtggaattaa tgatgcgaga ttattttcaa gccacaaaat ctatttcgca
     3121 actgagccaa ttattgttaa acagttttga acaagcctat gtaaacgagt tgcaaaaaaa
     3181 tgcacaaaaa caaccgcttg atcagtattt ttatcggcaa gaacagcaat tgtttgtggt
     3241 cgaacgcaaa atatttaaaa cccaacccac aacaatgttg gatctctttt ttcatcttac
     3301 taatgaccct acgttaatag cgaatgccga caccttacgt cagttgagac tgtgtttaca
     3361 gcaacaacac gagcctatgt gctttttgcc tgaaatgcga gagaaattcg tacagctttt
     3421 tgctcaaccg aaagtcataa gccgagcaat agttccaatg caccaactgg gttttttaag
     3481 tgcttatcta ccgcaatgga aggggatcga agggctaatg caattcgacc tattccatat
     3541 ttatacagtc gatgagcata cggtcagagt catgttaaat ttagaaaatt ttagcgaaga
     3601 tccaagtcgg gcgtttttcc cactatgtca tcagctattt gcaacgcttt ctgagaaacc
     3661 gttgctctat cttgcggctc ttttccacga tattggcaag ggtagagagg gcgaacacgc
     3721 tcaagttggt gcgctggata tgcgagaatt tgcggaacta cacggttttt ctcaagcaca
     3781 aatcaatgat atggtttggc tggtggcaga gcatttgaca atgtcgatca ccgcacaaag
     3841 acgagatatc cacgatccgc aaatcatcag cgaatttgca aaaaaagtgc aaaatcagac
     3901 cgctttatct tcactacttt gtttaacggt tgcagatatt tgtgcaacca gtgagagcct
     3961 ctggaatgat tggaaagcct cgctgtttac ccaattatat caatttacgc tacagcaact
     4021 ggctcaaaat ttgaattatc aagcagtggc acaagagcat cgcttgcaag cattagaact
     4081 gatgaagttc gtgctttcag ctgatgagcg aatagcatta aacgcatttt ggcaaccttg
     4141 cccggagcgt tattttcttc accatacacc gactcagttg gtatggcacg ctctgcatta
     4201 tgtaaaaact cgccggttgc cgatggtttt aatcagtaat gaatatgccc gtggtggtac
     4261 agaaattttt gtatgttgcc aagatcaacc tcaactgttt gctcgaatcg cccaattact
     4321 cagccaaaaa cgcattagca ttcacgatgc acaaattatt actactgaaa atggtgtagt
     4381 gttagacagc tttattgtaa gtgagcgtga tggtgatgat gatttaaccg atgagagatg
     4441 tatgcaaatt cagcaggcga tgatgaaaat gttaagctta actgaaacag gcataaaatt
     4501 taccaaaaaa ccggtgaaac atttgtcatt taagcgtaaa actcggctgc gttttcttcc
     4561 tccgtcatca aagcaacaaa ccgaatttga actttttacc ttggatagag aagggttgtt
     4621 ggcacaaata ggctatattt ttaaccggct ccgattaaat ttaattaatg cgaaaattac
     4681 gactattggt gaaagggtag aggatttctt tgtggtcagt aatgatcaag gaaaagcttt
     4741 atcggaacaa gaacagttag aactaaaagc cattctaatt accgaactag atagtgaatg
     4801 aaaacaaccg cttggagtga agactgcaag cggtaaattt ttcaacgttt taggtaaatt
     4861 aaatcacgag ttaagagagg ttgagaattc aagaaattag aaactaaata taagtttcta
     4921 tcgtttagat taataaatta agacgcaatt tttattaatt accatgatct tcaaacgtta
     4981 aatatgatga ataactaaag gtttgccttg gcattctaac acttcggcat caagattaaa
     5041 cactgttttt aaattttcag tggtgataat ctcttttggg gtgccgatca tcttaattcc
     5101 accatctttc attgcaataa tagtatctgc gtaagcagca gcaatgttga tgtcgtgtac
     5161 caccattata gtagttaagg cgagttcatc ggttaatttt cgtaataatt tcattaattc
     5221 ccgagcatgg aacatatcta aattattcag tggttcatct aataagatat gatcggtttg
     5281 ctgacagaag gtcattgcaa ttaatgcccg ctggcgttgc cctccggata attcatttaa
     5341 gaatcggtgg gagaaagtcg ataaatcaaa tcgtaccaga gcctcttcta caatggcatt
     5401 atcgtgcgaa gtgattctgc cttgatgatg agggtaacgc ccaaataaca gcagatcttt
     5461 gacggtaata cggctatgaa tcacattatc ttgggtcaag atagccagtt gtcgagcgat
     5521 agtttgagaa gctgtgttgg ctatattcag ctcatttaag tggatttcac cctgttggat
     5581 aggttgcaag cgtgcaatta aagagagcag ggtggatttg cccgcaccat ttgctccgat
     5641 taaagcggta ataccaccat ttggaatggt taaattgata ttattcagaa tcgtggcatt
     5701 cccgattttg tggtgaagat ttttaaccgt tatcattttc ttccttaatt tatggttatt
     5761 ttttccgttt taaaatgaga taaataaaga caataccgcc aataaattca ataacgacac
     5821 tcagaacccc ttgcattttc agcacttgct cgaagattgc ctgccctaaa attaaggtga
     5881 tggcagagat taaaaaagtc attggtattc gcacgctatg gcaaacagtc gggctaattg
     5941 cattaacaat ggcacaaaca agtagcccta gaaatagaat cgggccaaca agtgcggttg
     6001 aaacagacac caacaatcca caacagatta gtaattgacg tgaaaattga tgatattcta
     6061 acccaagccc aattgcctta tctttgccaa gtaacaaaat atctaattta tgccgttgta
     6121 accagaccca aatggcagag agaatagcga tacagcctcc aatccaaagt aatgtggggg
     6181 tagtcaggtt aaatgaggca aagaagagcc ttgagcaacg gcaaactctg ttgggtcgac
     6241 attcgttgca acaaattatt taggcttcta aataagacac caaagatgac accgattaaa
     6301 agcattcggg ttaaatcgga tcgatttcga ttcagtgaac taaatagtaa tattgatgcc
     6361 cctagcatta acaaggtttc aaagaaaaac ttatagcttg ggtctaactg agtaaagcct
     6421 aaaccgccaa ataaaaatac taacgaggtt tgcaatagca agtagagcga atcgaacccc
     6481 aaaatacttg gagttaaaat aggattatgt gtgagtgttt ggaatagcag ggtcgaaata
     6541 ccaatcgtat aagcaatagt acagagcaaa ataagctttt tgccacgcag ttgcaatata
     6601 aagtcccaat tacctttcgc attataggtc atataaaaca gaatacttgc taataagatt
     6661 aagcttaaaa cgataaagcg tgatctaacc acgagccttc tcctcagccc gtttctcttt
     6721 gagcaataaa tagaggaaaa tcactgtccc aataatccca aaaatagtag aaatcggcac
     6781 ttcataaggg gcattgatgg ctcgccctaa aatgtcacaa atcaggacta aattcgcacc
     6841 taacaccacg accgcaggca gatttttacg caatcggtca cctgctatgc gagagacaat
     6901 attagggacg accaagccga taaacggaat ctgtccaacc gtcaccacga caatagcggt
     6961 aatcatcgcc accacaatta aggcaagcca agtcatttgt ttgtagttaa tgcctaagtt
     7021 ggtactaata ttttcaccaa ggcctgcaat ggttaattga tctgccatca cataaaccac
     7081 aacagccaga atagcggtaa tccagagcag ttcataccga cctgccaaaa taccggagaa
     7141 gtcgccggaa aaccaaatcg aaagcacttg taggctgtca gtttcataag caataaaagt
     7201 gctgatagct tcaataacat tgccaaaaac gatcccgatt aatgggagca ttaattgttg
     7261 atgaggcggt aattgacgaa tgagcaacat aaaaagcccc attcccatta atgcacttaa
     7321 tgtggcaata gacattttta acagtaatgc tgatgttggg aaaagtaaac tagcaagtaa
     7381 aataccaatt gccgcacttt ggctagtgcc aatcatacta ggctcaataa agcggttctt
     7441 cagcacaatt tgtaatacca tacctgcaac ggctaaggtt gcaccgacta atatcactgc
     7501 gagcgttctt ggtaaacggc tgataaaaaa gatctgactt tgttcggaag aatgaaaaat
     7561 ttcactccaa tgaaaatctg ccacacccac actggcactg attggaatga gcaatattaa
     7621 aagaaataga tttaatctaa aaaaagagaa catagtgata aatagcaagc ggtcatattc
     7681 tctctctttt ttacaaatgc aggcacttgc aaaaatagga ggaaatctga ccgcttgtaa
     7741 ggttaattat tttttctcaa atgctgtttt aatgttagtt aggtcggttt tcatctgctc
     7801 ataaccaccc ggcgctaaat atgaggcagc acttagataa acgatattac cgtttttcca
     7861 agctttagtt tgacgaacta attcattatc caacacttgt tgggctgatt gaccttcttg
     7921 tccgatagcc gcaatacggt ctaacacaaa caaccaatcc ggattgagtt tttggataaa
     7981 ttcaaacgaa acaggctgac cgtgaccggt atttttaatg ctagaatctg ccattggaat
     8041 ccctaaatta tcgtgaatcc aacttaaacg ataacctgaa ccaaatgcag aaattttgcc
     8101 accattgact aaaataatca gtccgttacc tttacctttt actgctgctt tggtttggtc
     8161 taataaagta ttaatttctg cttttagttt ttcggtttcc gcttctttat taaataattt
     8221 gccgaaagaa tcaagacgct gtaatccgct ttcgattaat ttatcgccat tatccgtcat
     8281 gtcgatggtt ttcgcaattt ggctgacttc atcaaatttt tttgctgtgc gtgtgccaac
     8341 aatgatcaaa tccggattta aagaatttaa cgcttctaaa tccggctcaa ataccgtacc
     8401 gacattcacc gctttgtctg cactcggttt aagataagaa agcaccttaa attcaggcat
     8461 accgtcaatt tttacaccta aggcttgtaa agtatccaaa cttccggtgt caaacacggc
     8521 aattttagac ggggtttctt tgaaagtaac ctctccacgt gcagttggaa tagtaacatc
     8581 tttggcaaat gcagaagcag caaacacagc agttgcggtc aatgcaaaca aggctttttt
     8641 aatcattgga taactccatt tatattgaaa ataattctca aagattttaa cgaaaattga
     8701 ttttaagtaa agcggaaaat aacaagcggg cgaattttgc ctaaaatttg caaatttcaa
     8761 tttaattaaa attccgatac actatgtaca tcaatacgtc attatagcaa gagaaaaata
     8821 tggcagaata tagcggaaca acccaagaga agatgaaaga taagcaggtt gagcaaatta
     8881 atatctcacc acattcgatt gaggcagagc aagcggtgct tggtggcatt atgctcagta
     8941 atgatcactg ggataatgtt gctgagcgtg tacaaccgca cgatttttac aactatgcgc
     9001 atcgaattat ttttgaacaa atggcggagt tggtgcgcca caataaaccg attgatatta
     9061 ttacgctgga tcaagcctta aaagacaaag gggtgctgca agatgtagga ggttttgcct
     9121 atttagccga gctttctaaa aataccccga gtgcagcaaa tattttagcc tatgcggata
     9181 ttgtgcgaga tcgttccgat ttaagaagtg taatcagtgc cggcaatcaa attgcggaaa
     9241 tgggctacaa aacgaaaggg cgtaactcca aagaagtgtt agacgaggca gagcgaattg
     9301 tgtttgaaat tgcagaaaaa cgcaataacg ccaatgaagg tccacaacgt attgacgata
     9361 ttttgcttaa aacactcgct cgaatggata cgctatctaa aaatcgccat aacggcggag
     9421 ttactggtgt ttctaccggg tttgttgatc tcaataaaaa aaccgcaggg ttacagcctt
     9481 cggatttaat cattgtggca gcccgtcctt caatggggaa aactactttt gcaatgaact
     9541 tatgtgaaaa tgcctcatta gtggatattg aagataaaga tgatttagac gaacagggta
     9601 atccgaagaa aaaaccggca aagccagtgc taattttcag cctggagatg ccggcagacc
     9661 aaattatgat gcgtatgctt gcctcacttt ctcgggtcga tcaaaccaag atccgaaccg
     9721 gtcagatttc agatgatgaa gacactgcta aaatctccag cacaatggca attttacaag
     9781 aacgtaataa tatctatatt gatgatagct ccggtttaac accgaccgaa ttacgttctc
     9841 gagcgagacg gatttatcgt gaaaatggcg gattaagctt gattatggtg gactatctcc
     9901 aattaatgcg agcgccggga tttgcagata atcgtacttt agaaatcgca gaaatttccc
     9961 gttctttaaa ggcactagcg aaagaattag aagtgccagt agtcgctctt tctcagctaa
    10021 accggagttt ggaacaacgt gccgataaac gtccggtaaa ctcggatttg cgtgagtcag
    10081 gttctattga gcaagatgca gacttaatta tgtttattta tcgtgatgag gtctataacg
    10141 acaattccga tctgaaaggc attgcggaaa ttattatcgg aaaacaacgt aacggcccga
    10201 ttggtcgggt gcgtttaacg ttccaagggc agtattcccg ttttgataat tacacaggag
    10261 ggcattatga tgatgaatac taaaccaacc cagccaatca cactttgtgc gatttatcgc
    10321 agtaaggcaa aagaaggaat gtatttatat gtgcctaagc gtgaccaatt tgagcaagtg
    10381 ccggaaacat tacgccagtt atttggtaag cctgaatttg tgatgatgtt taatttaacc
    10441 ggtgaaaagc cattggtgcg tgctaaaaac gaagaagtgt tacaattact tatagagcaa
    10501 ggctactatt tgcaaatgcc acctccgccg gagaatttgc ataaagcctt tatcgcacaa
    10561 cagaaagggg aaaaataatg cgctgcccat tttgttccac tgaagaaact aaagtgatcg
    10621 attcacggct tgcagcaagc ggttatcaaa ttcgccgccg ccgtgaatgt ataagctgta
    10681 aagaacgttt tactaccttt gaatcggccg aattagttgt gccttatgtg atcaaaaata
    10741 atggtagccg agagccgttt gatgccgaaa aattaagaac agggttaaga cgtgcattaa
    10801 ataaacgccc tgtcagtgct gatgatgtcg aaaaagcaat tagccaaatc attattcagc
    10861 tgcaagcgat gggcgaacga gaagtgccga gtcgccatat cggtacattg gcaatgaacg
    10921 cacttaaaca attagacaaa gtagcatata tccgctttgc ctctatttat ttaagcttta
    10981 gtgatattga agagttcatt aaagaaattg aaaaattaaa agagtagttg caaaaaaaca
    11041 taaaaaaatg accgcttgtg agtttaaaat ccacaagcgg tcatttttct ttaaaaatac
    11101 tcaattagct tttacttgtt ccaacacaaa ccctacaaac gcccgcacaa tgctagacgg
    11161 ataacgatgg gcggcataaa tcatcgtcaa atcaaatttg gggttttctg tctcacccaa
    11221 gacttccacc aagccatttt ctgccaccaa tctatccacc gtgctgtcgg caagcagagc
    11281 cactgctttt tgccatatat tcaatggcat agctgtcatt gctggaaaaa agatagggaa
    11341 tttctaccgc ttgtctgcca gtgcctagcg tgatgacgct tgcttcattt tttgcccaag
    11401 cggcaatggg gaaatttttc aaatctttga cggtgctggg cgtgccaaag cgtgccaaaa
    11461 atttagggtg tgccacccat ttactgccga tttgccccac ttttttggcg ataaactgtt
    11521 cgccgtgtag ttgcccaatc cgaaacgcca catcaatgcc atctgccgac aaatccaaaa
    11581 gacggtcggt cagcgtgcaa tgcacttgta cttgtggata agctgcctga aaatcagcta
    11641 tccacgccaa aacaggctcg caggcagggc ttgccgacag gcgtaattta ccctttaaat
    11701 tgccttgctc ttcaaacaac acttctttgg cagtggcaag ctgttcaatg gacaaatgca
    11761 cctgttcata caggcgttgc cctgccattg tgggtttaac gcctgatttt tggcggtcaa
    11821 agagttgaat gttaagctct ttttccaaat cggcaatttg acggctaatg gttgcaatcg
    11881 gcacattgag tcgctcggag gctttggaga gactgccgtg ctggataacg gcgacaaaga
    11941 ggcggagggt gttgaggttc attgtttttc tccccaatgt tcaatttctt gaataaaatg
    12001 tttaatactg ccttggactt gttgattttt accatataga taataaacgc ctgaaagttc
    12061 ttgtaaaaat ttactttctt tataagcgtg tttttcaccc aacacagtcc aaactaactt
    12121 taacttatgc tcttttaaaa aatccaaaaa tttgtctttt ttaaccagta agctattttg
    12181 aaatttttca tttatctctg gattaaagca aaccaaatta ttttgtaaat caacccaata
    12241 accatcttta atcgcatttt tcattttcat tccgtgataa agcataggaa ttggcgcata
    12301 aaatgaaatg ccgtcattgc cataagtaga ttcccaagtg tgagaatcga cactaggtat
    12361 taccttaaat gaaggcgatt tattttccct atgtcgataa atttcttgcc actcatttaa
    12421 tgtattttcg gcaaaaaatt gatgaatatt cattccatag tgttcattac cttctggcat
    12481 ccatctttcc ataaaatctt gttgtgccaa ccaatttttt gcttttgaaa attgagattc
    12541 tttaactaaa taacttctaa tttgtaacca catttgccta tcataagttt ctttttgccc
    12601 aaattttgct aattcacgaa aatcatcgtg tctttctaga acaagccact gttcatcatt
    12661 aatcgttact tccaaaagat tactaacatc aggaaaatca tttattttta cccaa
//
LOCUS       DANLXQ010000068        12650 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.068, whole genome shotgun sequence.
ACCESSION   DANLXQ010000068 DANLXQ010000000
VERSION     DANLXQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12650
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.068"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            184..402
                     /locus_tag="PXD94_001451"
                     /pseudo
     CDS             184..402
                     /locus_tag="PXD94_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            complement(454..669)
                     /locus_tag="PXD94_001452"
     CDS             complement(454..669)
                     /locus_tag="PXD94_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286311.1"
                     /translation="MQTEKRGRGRPKSGLTLQELQAKSDAKRGVRLKSFKFHEDFIAQ
                     LEQLAEQHGISQTQVIVQAVEQFSKGA"
     gene            1214..1915
                     /locus_tag="PXD94_001453"
     CDS             1214..1915
                     /locus_tag="PXD94_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286312.1"
                     /translation="MSTQISTQTISFNNQALITFEQNGTHYTAMKPICENIGLTWHAQ
                     YERIKRDEILSQGILIIRIPTNGGDQQMICLPIEYLNGWLFGIDINRCKPEIRETLIK
                     YKKECYQALHDYWFKGKAERSQIAPIVEPKIPMELTKKEWLRFASMWYALYNSLEVLA
                     TLEKPLRAIGSPFGATAYTHATEYQTTLGVMKRILEPMLADFEVDPFEEAHYHKALTT
                     LRQYQPKGLGGLVRI"
     gene            2169..2345
                     /locus_tag="PXD94_001454"
     CDS             2169..2345
                     /locus_tag="PXD94_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286313.1"
                     /translation="MTEEDKEYLQTKIENEGFEYAFVSYSDFEEVQDEKFHGLRKAYL
                     KARSELAEYIDIED"
     gene            2433..2816
                     /locus_tag="PXD94_001455"
     CDS             2433..2816
                     /locus_tag="PXD94_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286314.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEEDQIRCPKTYAKL
                     REILEANEENVVRIGNVSISIGSTT"
     gene            2866..3087
                     /locus_tag="PXD94_001456"
     CDS             2866..3087
                     /locus_tag="PXD94_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL6286315.1"
                     /translation="MNIYTDFLSREEIEFITQCKQKNLIKKQLNIMGIPFKENANGFP
                     VVRRDYAQAKQRKPIATNDSDWVSNAFKA"
     gene            3109..4149
                     /locus_tag="PXD94_001457"
     CDS             3109..4149
                     /locus_tag="PXD94_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6286316.1"
                     /translation="MARPRKYENNGLPQNLLCRRRKRANGSIVEYYFYVLADGKERSL
                     GTNKHEAILEAAKLNFENSRKSPIVLFIDVAKRYELEVVPTKKAKNTRQSNLQAIHWL
                     CKFFGNPPAPLEKIEPKHISQYLQWRKDTPAVANIEVGLFNTIWNMAREWGYTALPSP
                     SQGVKKFPTKYREVYVEDYILDKIYEFADERMADIIETAYLLGQRPIDICNIHRSHIY
                     DGILHITQQKTGKKVRFEISGRLKEILDKRLGYESDWIFTNKWGKKLKRRSLGDHFKE
                     IRERAMKAYPELADEIAKVQMRDMRAKAATDISLMTTDEQAQKQLGHTSKRMTHHYIR
                     KDKILKPTDEIT"
     gene            complement(4308..4595)
                     /gene="rplY"
                     /locus_tag="PXD94_001458"
     CDS             complement(4308..4595)
                     /gene="rplY"
                     /locus_tag="PXD94_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL6286317.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(4747..6015)
                     /locus_tag="PXD94_001459"
     CDS             complement(4747..6015)
                     /locus_tag="PXD94_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL6286318.1"
                     /translation="MSDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAE
                     PCLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAG
                     IDVFDIEINETDPEKLVEIIASLEPTFGGINLEDIKAPECFYVEQKLRERMGIPVFHD
                     DQHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMC
                     DSKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTM
                     AKDLLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVG
                     AKTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIG
                     VAKAAIQSGVATRPITDWEAYAEQLKKLVA"
     gene            complement(6172..7083)
                     /locus_tag="PXD94_001460"
     CDS             complement(6172..7083)
                     /locus_tag="PXD94_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL6286319.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDQNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGDAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEMVSYFINKMKNA"
     gene            complement(7080..7799)
                     /gene="nfsA"
                     /locus_tag="PXD94_001461"
     CDS             complement(7080..7799)
                     /gene="nfsA"
                     /locus_tag="PXD94_001461"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL6286320.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQILIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGILAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(7811..8596)
                     /gene="mtgA"
                     /locus_tag="PXD94_001462"
     CDS             complement(7811..8596)
                     /gene="mtgA"
                     /locus_tag="PXD94_001462"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL6286321.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(8571..8891)
                     /gene="trpR"
                     /locus_tag="PXD94_001463"
     CDS             complement(8571..8891)
                     /gene="trpR"
                     /locus_tag="PXD94_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL6286322.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWLNGKVNGTV
                     NGKA"
     gene            complement(8971..9612)
                     /gene="lolA"
                     /locus_tag="PXD94_001464"
     CDS             complement(8971..9612)
                     /gene="lolA"
                     /locus_tag="PXD94_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL6286323.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSGDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(9723..12581)
                     /gene="polA"
                     /locus_tag="PXD94_001465"
     CDS             complement(9723..12581)
                     /gene="polA"
                     /locus_tag="PXD94_001465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL6286324.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDAKGKTFRDELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKRWLNEVNQNANPVTQTSAEKVPNNYQVTQAVRNEENVANVVEIDRSKYE
                     TVDTEQKLSAWIEKLTAARLIAVDTETDSLDAMSANLVGISFALENGEACYIPLGHKQ
                     KAQPQQADMFGDEERSESPSSDELVKNQLNLTACLSKLKPILLNPEIKKIGQNIKYDL
                     TIFANYGIELQGVAFDTMIESYTQNSTGRHNMDDLAARYLGHKTIPFEELAGKGKNQL
                     TFDKIEIAKAAEYAAEDADVTMKLHQVLWEKLQQEPELVKLFDEIEMPLVGVLSRIER
                     NGVLIDPKKLLTQSAEIEKRLEEVETLVHAEAGQTFNLASTKQLQEILFEKLGLPVLK
                     KTPKGAPSTNEEVLDELAQQGHLVPKLLMEHRGLSKLKSTYTDKLPQLINKKTGRVHT
                     SYNQAVTATGRLSSSDPNLQNIPIRNEEGRRIRQAFVAREGYKIVAADYSQIELRIMA
                     HLSNDEGMINAFAEGKDIHRATAAEIFGVELGEVTSEQRRSAKAINFGLIYGMSSFGL
                     SNQLGIGRAEAQKYMDLYFQRYPAVQQFMTDIREVAVEKGYVETLFGRRLYLPDIKSG
                     NAILRKAAERVAINAPMQGTAADIIKVAMIGIDNAIRDNDEIKMIMQVHDELVFEVKA
                     DKIDHYSQLIKTEMEKAISLKVPLIAEVGVGENWDEAH"
BASE COUNT         3785 a         2808 c         2377 g         3680 t
ORIGIN      
        1 ccttatattt ttttatgaga ttactacgaa agcgttttac tgtcagaaca aaaatttatt
       61 ttttcgatgc agatcgcaaa aatagatatt gaggcttgac accgccaaac ttcggattaa
      121 gataaccgca ctttcaagcc gtccaccaac ggcttttttt gtacctaaaa acggagaaac
      181 gaaatggcaa atagcgtaat gattgtacaa ggcgtggagg tcaaagtaac cgctcgtgat
      241 ggtgaggatt acatcagcct aactgatatg tgcaaagctt ttggagacag cgaccagctc
      301 attaaaaact ggcttcaaaa taaaaatacc attgaatttc ttcaagtttg ggaagagctg
      361 aataatccaa attttaattt ggtggaatta caccaaatta aatgcaacaa ctaattaaaa
      421 acatcgaaca gtgaagggta ggtttatagg ggcttacgcc ccttttgaaa actgctcaac
      481 cgcttgaacg attacctgtg tttgcgaaat gccgtgttgt tctgcaagct gttcaagttg
      541 agcaataaaa tcttcgtgga acttgaatga cttcaatcgc acaccacgct ttgcatcgct
      601 ttttgcttgt aattcttgaa gcgttaaacc tgatttaggg cgaccacgac ctcgtttctc
      661 tgtttgcatt gtcaattcct tgttgatttt taatacggaa cgttatatta tttaggaact
      721 gcctagcaga gatggcgttc tctgctagga cttttagctt tctaatctaa taagctggcg
      781 tgcttattaa gattaagatc actaggatta acaacttaag atacataatc cagttcctga
      841 tgtttgccgc tctcttcaag ggcggcttct tcattttcag accctttccg aaaacaagat
      901 tattttaata cataccaaat aaaacgcaag ctatttttta gtatttatta aaataatttt
      961 atttgacaac cgccaaactt taacttacta ttccgcccaa ggtgtcgaaa cctcatactc
     1021 taaggcggat agttcactaa tcgccactag gcgatttttt tatatccgta atcctgacta
     1081 tgtcgggagg gcgactaata caagaccgaa aggaaatacg tccagccctt accttagagt
     1141 gggttttcga acctcccgac gccactgtcg aaagtggctt gttttaaaca aatagactct
     1201 aaggattacg actatgtcaa ctcaaatttc tactcaaacc atttctttca acaaccaagc
     1261 cttaatcaca ttcgagcaaa acggcacaca ttacaccgca atgaaaccga tctgcgaaaa
     1321 tatcggcttg acttggcacg ctcaatacga acggatcaaa cgagatgaaa tcctttcgca
     1381 gggtattctt atcataagaa tacctacaaa cggtggcgat caacaaatga tctgcctccc
     1441 aattgaatac ctcaacggct ggctcttcgg cattgacatc aatcgctgca aacccgaaat
     1501 cagagaaacg ctcatcaaat acaaaaaaga gtgctaccaa gccctgcacg attactggtt
     1561 caagggcaaa gcggaacgtt cacaaatcgc cccgattgtc gaacctaaaa tcccaatgga
     1621 gctaaccaaa aaagaatggc tacgtttcgc ttcaatgtgg tacgccctct acaacagcct
     1681 tgaagtactt gctacgttag aaaaaccgtt gcgtgctatc ggttcgccgt ttggggcaac
     1741 cgcctacact cacgccaccg agtatcaaac cacgcttggc gtaatgaaac ggatacttga
     1801 accgatgtta gccgacttcg aggtcgatcc gtttgaagaa gctcactacc acaaagccct
     1861 aactacctta aggcaatacc agccgaaagg cttaggcggc ttagtccgaa tttaatttgc
     1921 aaaaaaatca cacaatccga ccgcttgtga aacatcaagc ggcggtttcc tgcacccaaa
     1981 attcaaggat taggcgatga aatacgcaaa aatcatactc tttttaaccg cctttgcagt
     2041 tgctgccgac tacttagagt tacacaacga ctgcgatggc aaaccatgca ctatcgcaca
     2101 gcgataatgc ttccccgacc ctgcccgtgc agtgtttatt tttatccact ctcaatagga
     2161 gaaacgaaat gacagaagaa gacaaagaat atcttcaaac taaaattgaa aacgaaggtt
     2221 ttgaatacgc tttcgtcagt tatagtgact ttgaagaagt ccaagatgaa aaattccacg
     2281 ggcttcgcaa ggcttattta aaagctcgaa gtgagttagc ggaatatatt gatattgaag
     2341 attaaccact gatgaactgt tcattgaaca aatgaacact tgacaaccaa ccgcttgtag
     2401 ctaactgcaa gcggttaatt tttggaggaa aaatgcacat agaagacaaa atcgcttggt
     2461 ggcttgccaa tggagagaca ggggtaagca gtaaaacaat ggctttttac cttggttacg
     2521 gaataagacc aaaaatagaa ggctacccac acgatgtatc ggatttcagg cgttgctttc
     2581 tgttgttgga gacagtccct tttttgcgaa ataggattga aaaaatggct gaacttggga
     2641 aagtttgggc tgcacttgca aaagaatggc acacattaga ggctctctac aacgaagagg
     2701 aagatcaaat aagatgcccc aaaacctatg ctaagttgag agaaatccta gaagccaacg
     2761 aagaaaatgt tgtccgcatc ggtaatgtga gcatttcgat aggttcaacg acttaacgat
     2821 tatttaacca aatccctcta aatgaggggt ttatttggag agaatatgaa catctacact
     2881 gacttccttt ctcgtgaaga gattgaattt attactcaat gcaagcagaa aaatctaatc
     2941 aaaaaacaac tcaacattat gggcattcca tttaaagaaa atgcaaatgg attccctgtt
     3001 gtccgccgtg attatgctca ggctaaacag cgtaaaccta ttgcaacgaa tgattctgac
     3061 tgggtatcaa atgcatttaa agcgtaaatt gagaagaagg aggcacttat ggcacgccct
     3121 cgaaaatatg aaaacaacgg attgccacaa aacctactct gccgccgcag aaaacgagca
     3181 aacggaagta tagttgaata ctacttttac gttcttgccg acggcaaaga aaggtctctc
     3241 ggtacaaaca aacacgaagc aattttagaa gcagccaagc taaattttga aaatagtaga
     3301 aaatccccaa ttgttttatt tattgatgtg gcaaaacgct atgaattgga agttgttcca
     3361 accaaaaaag ccaaaaacac tcgtcaatct aatttacagg ctattcattg gctatgtaaa
     3421 ttctttggta acccacctgc accgctcgaa aaaattgagc caaagcacat cagtcaatat
     3481 ttacaatgga gaaaagacac gccggctgtt gctaatattg aggtaggttt atttaacaca
     3541 atttggaata tggcaagaga atggggatac actgctcttc caagcccatc acaaggtgta
     3601 aaaaaattcc cgaccaaata tcgtgaagtt tatgtagagg attatatcct tgataaaatc
     3661 tatgaatttg cagatgaaag aatggcggat attatcgaaa cagcctattt gctgggacaa
     3721 agaccgattg atatttgtaa tatacaccgc tcacacatct atgacggtat attgcatatt
     3781 actcaacaga agacagggaa aaaagtgaga tttgaaatta gtggtcggct aaaagaaatt
     3841 ttagataaaa ggttgggata cgaatcagac tggattttta ccaataaatg ggggaaaaaa
     3901 ttaaaacggc gaagtttagg cgatcatttt aaagaaataa gggagagagc aatgaaagca
     3961 tatcctgaac ttgctgatga aatcgctaaa gtacaaatgc gtgatatgcg tgccaaagca
     4021 gccaccgata tttccctaat gacaaccgat gaacaagctc aaaaacaact tgggcataca
     4081 tcaaaaagaa tgacacatca ttacatcaga aaagacaaaa tcctcaaacc taccgatgaa
     4141 atcacctaaa aataaagccc ttttgccaaa tacaaaagag ccatttactc acctaaaatc
     4201 cgttccgaaa ctattttgta aataattgat tttattgacc taaaaaacta taaaatcgca
     4261 aaaagtttcg gaacagaaaa cagcgttaaa cccttgtaaa tcaaggatta agcacgtttg
     4321 aagtctaagt gaactaattt tggtttagtt gggtggcgtt gaattgcttg tacttttact
     4381 tgctcttctt tacctgcaac aacgatagtt aatacttcgt tatagaatga atcgtgaact
     4441 tgtgcgttgt tcactttatc gtgatctaaa atgattgata caggctctgc attaccaccg
     4501 tagatgattg caggaacttg accattgtga cgcaggcggc ggctcgcacc cttaccttgc
     4561 gcagaacgaa cttcagcttc aaatttgaat gacatagttt tattctctta aataaaatgg
     4621 ggaaatcccc gtgaatagaa aaaacagcag gcgacccagc tgtttccgaa aaaatatcgg
     4681 catatccgtc tcgatatgcc aactacgtat tttactgaag ttgcagaaaa atgcaatgta
     4741 aaaatattaa gcaaccagtt ttttcaactg ctctgcataa gcttcccaat ctgtaattgg
     4801 tcgagttgct acacctgatt gaattgctgc ttttgctaca ccaatcgaga cattcacgat
     4861 caaacgtgga tcaaatggcg ttggaataat atattccggg ccaaacgata attcgccata
     4921 agttgaaata acttcaaacg gcacaggttc tttagccaac tctgcaatcg catgggctgc
     4981 tgccattttc atttcttcat taatggtttt tgccccaaca tctaatgctc cacggaataa
     5041 gaacgggaaa caaagcacat tattcacttg gtttttaaag tctgaacgac cggtacatac
     5101 aatcgcatct ggacggacag ccttcgcttc agcaggtgta atttccggca cagggttcgc
     5161 taaagcaaga attaatagat cttttgccat tgttttaacc atttcttgcg ttaaagctcc
     5221 gccttttgaa caacctaaga aaacatctgc attcgcaacg gcatctgcca atgttctcca
     5281 accattatct tctattgcat agaattgttt ggtttcatcc atcttatcac cacgaccttg
     5341 gaaaataacg cccttagaat cgcacattgt gatattttct tttttcacac ctaatgaacg
     5401 gagtaagtta gtacacgcaa tcgctgatgc tccagcaccg tttaccacta aacgtacttc
     5461 tgaaattgct ttacctacca cttgtaatcc gttaatcacc gccgcaccgg taataattgc
     5521 cgtaccgtgt tgatcatcgt ggaacacagg gattcccata cgttcacgta atttttgctc
     5581 tacataaaaa cattcaggtg ctttaatatc ttccagatta ataccaccaa aagtcggttc
     5641 taacgatgca ataatctcca ctaatttctc cggatcagtt tcattaattt caatatcaaa
     5701 cacatcaatt ccggcaaatt tcttaaatag aacacctttg ccttccatca caggcttact
     5761 tgctaaagca ccaatattgc ctaagcctaa cactgctgta ccattagaaa ttactgcaac
     5821 taagttacct cgtgccgtat aacgataaga agctgaagga tctttttcaa tcgctaagca
     5881 aggctcagca accccaggag agtacgctaa agctaaatcg tgctgagtat ctaatgcttt
     5941 agtaggagta acggaaattt ttccggggat tgggaactcg tgaaaatcaa gcgcctgttg
     6001 ttttaaatcg ctcattttag aaaccttata atctataaaa ataaaagtaa tcaataatag
     6061 ccaaagagta gccattttac cctaaatttt ttcgcatttt tatggctttg ctctcatttt
     6121 ttatgatatt tacaaaaaat aatacaaaaa cgtccgcttg ttttactcaa atcaagcatt
     6181 cttcatctta ttgataaaat aagaaaccat ttcagaagct atatcaacat catttactct
     6241 ctctatgcct tcaaatcctg ggctggcatt gacttctaaa ataactacgc ctttctctgt
     6301 ccgcagaaaa tcaacgcctg caatatcaag ccctatcgtt ttagttgctt taattgctaa
     6361 cgcacgttcc atctgagtta attcaatcgg ttgagccgtt ccaccttgat gaatattggc
     6421 tctaaaatcg cctgttacgc tggttcggct catggcagca accacttcat tgccaatcac
     6481 aaacgcccga atatcttgcc ctttagcgtc gcccacaaat tgttggcata gataaggctc
     6541 atttacttgc ctaaaggtcg ctaacacggc ctcggcatta tttttgcctt caaatagcat
     6601 tacgccattc ccctgtgaac catttagcac ttttgaaata agtggaaaag caaattgatt
     6661 gagttgtgat ttcacagaaa ccaaatgccc tgcaaaattc gtattaggaa ccggcaaatt
     6721 gtgtttggct aatttttgta atgttcgcca tttgtctcgg gctaacgcaa atgccgctga
     6781 atgatttaga acaggaatac ctttcgcctc aaaatggcgt aacaccgaac agcccatttc
     6841 ggtactgcct gtgccaaaac ggggtaaaat gccgtcataa ttcggcaaca ataccggctc
     6901 cggacggttg ttatcatagc tctcgccagc ttgatagtag atcttaaatt cgccattttc
     6961 taacccgagt agcattctat tctggtctaa aatatccagc tcaatcccct ttgcctcaca
     7021 agcggctttt attcgacagc agctatataa acgaggctct cgacatagca ttagccactt
     7081 cattttttca gcaatccttt ctgattgaaa aacggcaaaa tatgcggacg aacaggctta
     7141 tccaatgttt tatgaatagc tgcttgccat tgttgtggct cgccgattct ggcttggtag
     7201 taagcggtca aattttcatt atattttgca aattcagtcc aatcaaaatc ctgatattga
     7261 ttgttataac aaaccaaact attcggcaaa cgaggtttta ttgccggctc ttgtgccgga
     7321 taccccaaac acatacccac cagtggaaca caatattccg gcaagttaag ggctttagaa
     7381 acccgctcaa tatcattacg caatgctcca atatacaccg ctcccagccc tagtgattcg
     7441 gcggctaaaa atacattctg ggccaaaata ccggtatcta ccgccccaat caacgacacc
     7501 tccgcccatt ccaactgcga ttcagggaca agttgtttgt gtttattaaa atcaatgcag
     7561 aacaccaaaa attcagcaca atctactaca tattgcatac ctgaaacggc ttttaatgtt
     7621 tctcgaatct gccgatcggt tacacgaatc accgtcacac attgcagatg atttgaactt
     7681 gaagccgctt tacctgcctc aattaagatt tggaaaatct cttcagaaat aggctgttcg
     7741 gtaaatttac gaatagaacg gtgagataaa atcgtctcaa gagtcggttt actttgcata
     7801 tttttctcgt ctaaagttta tctaaataat ttttgccatc aagcaaactt acttgtcgca
     7861 taatccacgc ttggcgtttt cgcatactcg ggccgggttt atctacccta aacaccaacg
     7921 ggttaggcaa agaggctgcc aatagtgcgg attcttgcaa ggttagctgt ttggctgatt
     7981 ttttgaaaaa atgctttgcg gctgcttcca ccccgaaaat gccgttacca aattccgcaa
     8041 tattgaggta cacctctaaa atccgatcct tactccacac attttcgact aacagggtta
     8101 aaggcacttc aatccctttg cgtaaccagc tttgcccgtg ccacaaaaac atatttttta
     8161 ccgtttgttg cgaaatggtt gaacctccac gcactttttt ggatttagca ttcagtttca
     8221 aggcagtttc tatcgcttgt aagtcaatgc caaaatggct ttcaaacttt tgatcttctg
     8281 aggcaatcac agccatttgc atttgccacg aaatctcatt caggctcacc cattcttttt
     8341 tgatcgggta agatttcccc tgaaccagat tctccacctt tttttgcacc atatacgcag
     8401 aataaggcac cggtaacacc gaaaaaatca gtatcggaat caggaaaaat gtgccaccaa
     8461 aggttaataa gcgactagaa ccaaaccaaa gccagcccag tttacttttt attttgcgag
     8521 gaataaaaaa aagccctatt ttcttcaaaa gagagcttaa aaatcgcttg ttacgctttg
     8581 ccattgactg ttccattaac cttgccgttc agccattgca gaaaatcttg gtcgagtgtt
     8641 ttaagcatat ttgaaccacg agtgatggtt gccgcactgg tatttaaatt ttgttggatt
     8701 tcccgttgcg aatcttcacc ttttagcaaa gcttgcacaa tttgtaaacg caagcctaat
     8761 gaacctcgct catcggcggt gagaaacatt gagaaaaacg gctcaagttt atcttccgca
     8821 accgcttgtt caagcagagc caaaaactgc gtccactcgt ttggatctcg ctggttatag
     8881 agtgttttca tactgtccct tgaagtaaaa acggtgacaa gcggtcagat ttttacaaaa
     8941 ttttgcagaa aaacgaccgc ttgctctcca ttattttttc gattttttac cgccacgttg
     9001 gtcgtctaac tctgcccctt tcggtacgct gaatttaaac aaatcgcttg agacaccgcc
     9061 ggtagtaata ttgcgtaaca catataagtt actttgcccg tcacgctcaa tggtactgaa
     9121 accttttaat aaaccgcttt ggtcaatccg cacatcaaac tgttttaaat tgcttttttt
     9181 cgattttggc ttaagcacga aagtatcagc actctggctg acatcatatt gatcccagtg
     9241 gcttttatca ccgctggtta ataagacgaa tggcgtatta ttaactgcat cttgcacggt
     9301 atttaccgta acttgtgaaa cgaaaggatc gtaaaaccat aaatctttgc cgtcagatac
     9361 aatcaaattt tcttgtggtg atttggtatc cattctaaat aagttcgggc gttttacttg
     9421 gaatttgcct ttgccttgct gaatttgttt acctttgcta gaacgcacgg tttggtcgaa
     9481 atccgcacta tattggctta cttgttccaa acggcgttgt aattctgcaa cagattgagc
     9541 atcagccaat gccacatttg ccaagccaaa taacgctaaa ccgaataccg attttttaaa
     9601 taatgttttc atttgttttc ctttttacca acaagaattt tcttgtttga caataaaatc
     9661 atcaaaagtt tcatattaat gaactgttac aacaatacaa taaaagtcgt gaattgtata
     9721 gtttaatgag cttcgtccca gttttcgcct acgccaactt cggcaatcag tggcactttg
     9781 aggctaatcg ctttttccat ttcggttttg attaattggc tgtaatggtc gattttatcg
     9841 gctttgactt caaacaccaa ttcatcgtga acctgcataa tcattttgat ttcatcatta
     9901 tcacgaatcg cattatcaat accaatcatc gccaccttaa taatatcggc agccgtgcct
     9961 tgcattgggg cgttgattgc cacccgttct gctgctttac gcaaaatagc attgccggat
    10021 ttaatatcag gcaagtacaa acggcgacca aatagggttt caacatagcc tttttcaact
    10081 gcaacttcac gaatatcggt cataaattgt tgaaccgccg ggtaacgttg gaagtagaga
    10141 tccatatatt tttgagcctc tgcacgccca atccctaatt ggtttgaaag cccaaatgac
    10201 gacattccgt aaatcagccc gaagttaatt gctttcgcac tgcggcgttg ttcggaggtg
    10261 acttcaccta actccacccc gaaaatttcg gcagcggtag cacggtgaat atctttgccc
    10321 tccgcaaagg cgttaatcat tccctcatca ttcgataaat gagccataat ccgtagctca
    10381 atttgtgaat agtcggcagc gacaatttta taaccttctc tcgcgacaaa agcttggcga
    10441 atccgtctgc cctcttcatt gcggatcgga atgttctgca aattcggatc gctggaagaa
    10501 aggcgtccgg ttgcggtgac tgcttgattg taagaggtat gcacccgccc tgtttttttg
    10561 ttaatcagtt gtggtaattt atcggtgtag gtagatttca acttacttaa cccacgatgc
    10621 tccattaata acttcggcac taaatgccct tgttgagcca actcgtctaa cacttcttcg
    10681 ttggttgagg gtgcaccttt tggagttttc ttcaacaccg gcaagcctaa tttttcaaac
    10741 aaaatctctt gtaattgttt agttgaggca agattgaagg tttgccctgc ttctgcgtgt
    10801 actaatgttt caacttcctc caaacgtttt tcaatttcag ccgattgggt caataatttt
    10861 ttcgggtcaa tcaacacgcc attgcgttca attcgagaaa gtacgcccac aagtggcatt
    10921 tcaatttcat cgaataactt caccaattca ggctcttgct gtagtttttc ccacaatact
    10981 tggtggagtt tcatcgtcac atcggcatct tctgccgcat attcagccgc ttttgctatc
    11041 tcgattttat cgaaggtcag ttgatttttc cccttaccag caagctcttc aaacggaatc
    11101 gttttatgcc ccaaataacg ggcggctaaa tcgtccatat tatgacgacc ggtgctgttt
    11161 tgggtgtaag attcaatcat cgtatcaaaa gcaacacctt gtagctcaat gccgtagttc
    11221 gcaaaaatgg ttaaatcata tttaatattc tgcccgatct ttttaatctc cgggttaagc
    11281 aaaatcggct tgagtttact taaacaagcg gtcaaattta gctgattttt taccaattca
    11341 tcggaagacg ggctttctga acgctcctca tcaccgaaca tatcggcttg ttgcggttgg
    11401 gctttttgct tatgcccaag cggaatatag cacgcctcgc cattttccag tgcaaaggaa
    11461 ataccgacca aattagccga cattgcgtca aggctgtcgg tttcggtatc caccgcaatc
    11521 aaacgagctg cggtgagttt ttcgatccag gctgataatt tctgttcagt atcaacggtc
    11581 tcatatttgc tacgatcgat ctcgaccaca tttgcaacat tttcctcatt tctgaccgct
    11641 tgtgttactt ggtaattatt cggtactttt tcagccgaag tttgagtgac cggattggca
    11701 ttttgattca cttcgttcag ccagcgttta aattcataat gctcaaataa ttgagcaagc
    11761 tcagcacggt cttgcggctg tgttaaaagt tgatcgtgag taacatcaag ttccacatcg
    11821 gttttgatgg tagcaagcaa ataggataaa tcggcattcg ctttttccgc ttccaatttt
    11881 ggggcgaaat tttttgcccc acggaaagaa agggtggcaa tttgatccaa attagcataa
    11941 atctgttcaa gtgaaccaat gccttgcaat aaagctaatg cggtcttttc ccccacacct
    12001 tttacgcccg gaatgttatc ggcggaatcg cccattaatg ccagaaagtc gataatcaac
    12061 tcaggcggaa tgccatattt ttcaatcacg ccttcacgat ctaacaaagt attgttcatc
    12121 gtgttaatca acataatgtg atcgttcacc aattgtgcca tatctttatc gccggtgctg
    12181 atcaacacat ctttgccgtc tttagcggct tgcacagcaa gcgtgccgat cacatcatca
    12241 gcttccacac cttcaatcga aatcagtgga atgcctaagg ctttgataat gcgatgcaaa
    12301 ggctcaattt gagggcgtaa atcatcgggc attggtgggc ggtgcgattt atattgctta
    12361 aacaactcat cacggaaggt tttccctttg gcatcaaaca ccacagcaat atggcttgga
    12421 ttcacttgtg aaatcaggct ttttaacata ttcagcacgc catacattgc ccctgtcggt
    12481 tcgcctaagc gattagtgag cggagggaaa gcgtgaaatg ctcgatataa ataggaagaa
    12541 ccatccacca ataccagtgg attttttgcg atagttgcca taaaaatctc tttataatca
    12601 aataaatatt ggcgttatta tacaagaata tttaagaatt gagtgtgtat
//
LOCUS       DANLXQ010000069        12602 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.069, whole genome shotgun sequence.
ACCESSION   DANLXQ010000069 DANLXQ010000000
VERSION     DANLXQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.069"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            5..1450
                     /locus_tag="PXD94_001466"
     CDS             5..1450
                     /locus_tag="PXD94_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6286325.1"
                     /translation="MVSTLLILTSNLAFAETTPAQLQEKMDNQRMQKNLHTLEKPEPQ
                     AKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQLPAS
                     KQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENRENEAA
                     EAQFYKLQADKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGTTYG
                     NWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRESLG
                     LGYQNAKQKITLLPFFEQMWYAGGSSQTESTKRYSNAGGVNARWQYWITPQWQSAISY
                     EYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRRGVS
                     IGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKGITP
                     RLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
     gene            complement(1498..4095)
                     /gene="mutS"
                     /locus_tag="PXD94_001467"
     CDS             complement(1498..4095)
                     /gene="mutS"
                     /locus_tag="PXD94_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL6286326.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNSQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTPRQALDE
                     LYRLKKLLG"
     gene            4245..4808
                     /locus_tag="PXD94_001468"
     CDS             4245..4808
                     /locus_tag="PXD94_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL6286327.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            4982..5611
                     /locus_tag="PXD94_001469"
     CDS             4982..5611
                     /locus_tag="PXD94_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL6286328.1"
                     /translation="MAVSATSFAQTTQPTAAEQMNAVKASVAEKATVAKEATKEKAAE
                     LKAGSAEKMAIAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTMKVKAQE
                     AKAATAEKAATAHETLNSAKTATAEKAASMKAAATEKMSAVKKSQKVNINTANEEQLQ
                     SLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            5759..6388
                     /locus_tag="PXD94_001470"
     CDS             5759..6388
                     /locus_tag="PXD94_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6286329.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAETVARG
                     IYDVLNNNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAEASIPPFATLVFEVELLDIL"
     gene            6454..6969
                     /locus_tag="PXD94_001471"
     CDS             6454..6969
                     /locus_tag="PXD94_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL6286330.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(7017..7947)
                     /locus_tag="PXD94_001472"
                     /pseudo
     CDS             complement(7017..7947)
                     /locus_tag="PXD94_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(8006..8656)
                     /locus_tag="PXD94_001473"
     CDS             complement(8006..8656)
                     /locus_tag="PXD94_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha4 family transposase"
                     /protein_id="HDL6286331.1"
                     /translation="MRKSRLSQYKQNKLIEQFVAGVTARTASELVNVNKNTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEVDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQFATLLPIIREKVKPDSIVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFGSQAKRHLRKFNGIPKVHFELYLKECEWRFNHSDLKSQISFLKQLVKGSLG"
     gene            8985..10343
                     /gene="mnmE"
                     /locus_tag="PXD94_001474"
     CDS             8985..10343
                     /gene="mnmE"
                     /locus_tag="PXD94_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL6286332.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            10344..11609
                     /gene="tilS"
                     /locus_tag="PXD94_001475"
     CDS             10344..11609
                     /gene="tilS"
                     /locus_tag="PXD94_001475"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL6286333.1"
                     /translation="MALFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLMGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSNELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELPYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(11599..12492)
                     /gene="xerD"
                     /locus_tag="PXD94_001476"
     CDS             complement(11599..12492)
                     /gene="xerD"
                     /locus_tag="PXD94_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL6286334.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGVVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
BASE COUNT         3773 a         2933 c         2685 g         3211 t
ORIGIN      
        1 aaaaatagtc tcaacgcttt taatccttac ctcaaacctg gcatttgcgg aaaccacacc
       61 ggctcaactt caagaaaaaa tggataacca gcgaatgcag aaaaacctac atacattaga
      121 aaagcctgaa cctcaagcca aaccacaagc ggttaatgca caggctcacc ggcaatcgat
      181 ctctatttca agccaacagc tgcttcaaca gcctgagttg ctcaactcgg caatgctaac
      241 tgcgttggtc actaaccaca ctgagaatgt tcaattctta ctgcctattt atcaacaatt
      301 acccgccagt aaacaaaaca gcgaagttgt acaatgggcg gaggcagtta atgccaaagc
      361 aaaacaaaat tattcagagg caataaaagg ctaccgtcag ctactggcaa aataccctga
      421 aaatcagcca atccgtatgc aattagcggt tgcattgttt gaaaatcggg aaaatgaagc
      481 cgcagaggct caattttata agttacaagc ggacaaatta ccgcaagaaa ttgcaaatgc
      541 ggtcaatagc tatttggagg cgattggcaa gcaagatcaa tggagttttc aaggcggctt
      601 aacctatttg aatgacccga atatcaacaa tgctccaaat gcaggcacga cctatggcaa
      661 ctggactgca ccgaaaaaag aatccgccca aggggtcggc tttcactttg aggcggataa
      721 aaagtggtct tggggcaatg gtttcttcca cgagtttcgt ttaaacggaa acggcaaata
      781 ttactggaat aacaaaaaat ataacgaata ttcgctcaga gaaagcctcg gcttgggtta
      841 tcaaaatgca aaacaaaaaa tcaccttgtt gccgtttttt gagcagatgt ggtatgccgg
      901 tggctcaagc caaaccgaaa gcaccaaacg ctactccaac gcaggcggtg tgaatgcccg
      961 ctggcaatat tggattactc cacagtggca atctgcaatc agctacgaat atgcggaaca
     1021 gcgttacact cgccgccaac atttagacgg caattatcat tttattgccc cgagcctcta
     1081 tttttaccct aacagccggc aatattggtt tgtgggggcg aattttaacc gcaccagccc
     1141 ccgtgattta gatgatagct ttattcgccg aggtgtttct attggttggg gacaagagtg
     1201 gaaaaacggg ctttcaaccc aattaagtgc cagctacgcc cgcaaacaat accgagcccc
     1261 aatgccgatt ttccaaatta cacaacgtaa cagtgaatat ggtgtgcaag ccagcatctg
     1321 gcacagagcc ttgcatttta agggtattac ccctcgctta acgtggcaat ataacaaggt
     1381 aaaaagcaat catagcttct atagttatga caaaaaccgt gtctatattg agttttcaca
     1441 tacgttctaa acaaaaacgc cccttaaacg gggcgatttc ttagttattt tttcaattta
     1501 ccccaacaat tttttcaagc gatacaactc gtccaaagcc tgtcttgggg taagttcatc
     1561 aggctgaagg gctttcaggg ctttttcaac ttcattttct actacaagcg gttgaattgc
     1621 ttcatttttt tgcaaatcaa aaccatcgga aaacaacaaa tccgcctgtg gatgttcgtt
     1681 tgcttgtttg gtttggtggg aaatttcctc caaatgtgcc aaacgctgct ttgccagttg
     1741 aatcacttgc tttggcacac ccgccaaggc tgccaccgcc aagccgtaac ttttgctggc
     1801 ggccccctct tgcaccgagt gcataaacac aatactgtct tgatgttcac gcgcgtctaa
     1861 atgcacattt gccacccctt tgagctgatt cggcaagccg gtaagttcaa aatagtgggt
     1921 tgcaaacaaa gtgagtgact gggttttctt cgccagccat tcggcacacg cccacgccag
     1981 tgataagccg tcataggtag aagtgcctcg cccgatttca tcaatcagca caaggctgtt
     2041 ttcggtggct tgatgcagaa tattcgccat ttcggtcatt tccaccataa aggttgaacg
     2101 ccctgaagct aaatcatcgc ttgccccaat tcgggtgaaa atacggtcaa tcgggccgat
     2161 ttccgcactt tctgccggca caaaactgcc gatatacgcc atcaatgtaa tgagggcaat
     2221 ttgccgcata taggtgcttt taccgcccat attcgggccg gtcacaatca ataaatgccg
     2281 ttgggaattt aaaaacaccg gattggcaat aaacggctcg gttaaaacct gctccaccac
     2341 cgggtgtctg ccgcctttaa tattcaccat tcgcatcggg ctaaagctcg ggcaaacata
     2401 gcctaaacta tcagctcgct cggcaaggtt ggttaagaca tcaagttcag acagcatcat
     2461 cgccgctaat tgcaattcgc ctaagcgagg cattaacaca tcaaacagtt catcataaag
     2521 ctgtttttca agggcaagcg atgccccttt tgctttcagc actttgtctt cgtaagtttt
     2581 cagctcaggg ataatatacc gctccgcatt tttgagcgtt tggcggcgaa catagtgaat
     2641 cggggctttg tgtgcttgcc cttggctgat ctgaatgtaa tagccgtgaa ccgcattaaa
     2701 gccgattttc agcgaatcaa tgccggtgct ttcacgttct ctgatctcca aattttccag
     2761 atattgcgtt gccccgtccg aaagttgccg ccattcgtca agctcggcat tataaccttc
     2821 tgcaatcacc ccaccatcac gaatgagttg tggtgggttt tcgataatcg cccgctccag
     2881 caaatcgtgt aacgggctga aatccgccag ttgagccaaa agctgatcga tactagcggt
     2941 cagattttgt gaattttttg caatttccgg taactgagcc aatgcagtgc gtagtcgggt
     3001 gaggtcacga gggcgagccg tacgcaacgc cactcgggcg agaatccgct ccatatcgcc
     3061 gacttgttgt aataacggct gtaaatccga cattcgctca tattgtatca atgctgttat
     3121 cgccttttgt cgggcttgta atttaccaca atctcgaatc ggctgatgaa tccaacgctt
     3181 gagcagacgg ctccccattg gggtcacaca tttatctaac accgccgcca gcgtgttttc
     3241 cgtgccaccc gctaagtttt gggtcagctc taaattgcga cgagtagctg catcgagcaa
     3301 aatagtatcg ctatgttgtg ccaaatgaat tgcgttaatg tgcggtaaag cggtgcgttg
     3361 cgtttcttgg gcataatgca agacacagcc ggcagcacaa agtgccacca ccgctttttc
     3421 caccccaaat ccggcaagtg tttgcgtgcc gaattggcga tttagcagct gaatcgccgt
     3481 taccagctca aattcccaca ccggacggcg gcgtaaccct ttgtaattcg ttaaaattga
     3541 ggcagcggaa aaatcttccg catacagaat ttctgccggt tgtacacgtt gtaactcggc
     3601 ggatagagcc tctttgctag caagttcact gattaaaaac cgcccggagg tcatatctag
     3661 cgtagcaatc gcaaatgtgc ctttttcttc gtaaactgcc gccaccaagt tatcttggcg
     3721 ttcgggtaac aatgcctcat cgctaactgt tcccggcgta acaatccgta ccactttgcg
     3781 ttctaccggc ccttttgagg ttgctgggtc gccaatctgt tcacaaattg ctaccgattc
     3841 ccccaacgcc accaatttcg ccaaataccc ctccactgcg tgataaggca cacccgccat
     3901 cggaatcggc tcaccggcag attgccctcg cttggtgagc gaaatatcca acaatgctgc
     3961 cgctcgtttc gcatcatcat aaaacagctc ataaaaatcc cccattcggt aaaacaatag
     4021 tatgtccgga ttttgagctt tgagttgcaa atattgttgc atcattggcg tatgggtgga
     4081 gaaatcttgt gtcatcagtc tatttattgt gagaaatcat tgctgatagt ttaacagatt
     4141 cagccaaggg ataaaaacaa gcggtcattt ttcgctgaaa atttgcaatg gtcagccgct
     4201 gtttttttgt tacaatgcac caaattttta aaacagaggt cagaatgtta agaaaaatag
     4261 tgctactttc tacaggctta ttattggcgg cttgtagcag caatgaaccg caacaaccgg
     4321 aagcattccc gggcgaattt gccaatgccg attatgtgct ttcggaagaa aatgctcaaa
     4381 aatgggtaat tgccagcgaa caggcaaaac agtgtatcta ccccaattta acccgtattc
     4441 agcaagagca tttttccaaa gaagattctt atattttctc acaatatgta tttttctatc
     4501 cgcttgaaga gattatcggg gagcagtatg taaaaatcat tcaaaatgat gaaaaatcaa
     4561 tgggttacgc tcaatattta ttcaaaaaac tgaaaaataa cgaagcggtt gaagcgttac
     4621 cgaaaaaaca atgtgcgacc ttacgcacca atgcaaaaaa tgatttggca gtggtaaaag
     4681 ggcagtatca aagcggtatg atcgaagaaa ccaatagcca agaagggaaa tcaggcgata
     4741 aagttgccac caatgataat aaattcttct tcgatattat caaatggggc agtatgttac
     4801 ttctctagtg agcctcaata ctcggttgtt ttaaccacaa ccgagccatt tatacctacc
     4861 gattgaactt ttatctaaag cctcagtctt aaaatacagt ttttttcaat tattttccct
     4921 cttttatatt taaggatttt caataaaagc attaaaaaca ttattagcat tgggcgtggc
     4981 aatggctgtt tcagctactt catttgcaca aaccactcaa cctactgcgg ctgaacaaat
     5041 gaacgcagtt aaagcctctg ttgccgaaaa agcaactgtg gctaaagaag caacaaaaga
     5101 gaaagcagcg gagttaaaag ccggttctgc ggaaaaaatg gcgatagcaa aagccaaaac
     5161 cgaatcattt aaagattccg ctaaaaacaa aatggacgca gtaaaagcca ccacagcaga
     5221 aaaaacggac gtgattaaag cgaaagccgc atctgcaagc gacacaatga aagtgaaagc
     5281 tcaggaagca aaagccgcca ccgcagagaa agctgcaact gctcatgaaa ccttaaattc
     5341 agccaaaacc gcaaccgctg aaaaagcggc ctcaatgaaa gcagctgcaa cagaaaaaat
     5401 gtcagcagtg aaaaaatcac aaaaagtgaa tatcaacact gccaacgaag agcaattaca
     5461 atcgcttacc ggcattggtg aagcaaaagc caaagcgatt gtagaatacc gcaaaaaaca
     5521 cggtaaaatc aaaaatgcag cagaactggc gaacattccc ggcattggcg atgcaaccat
     5581 tgagaaagtt gctccatatt taactttcta atgttctgca agcggttatt ttgttgcaaa
     5641 attttgcaga aaaataaccg cttgttcatt tcacaccttg tattcttaaa tttcaccccc
     5701 attaagtgaa tcgtatttct ttcaatacag tattcaataa actcaaaagg aacagaaaat
     5761 ggcattagat ttcaattcag ttggattaga ttcagtagaa gcaaaaggcg gttacggcat
     5821 cggtttacaa attggtcagc aacttttagg cagcggaatg gacgtaaacg cagaaaccgt
     5881 tgcccgtggt atttatgatg tgttaaacaa caatgagccg gcaatcgaca tcaatgaagt
     5941 gaccgctgca ttacaagaat taggtcagcg tgcggaagcg gctcaagcag aagcattcaa
     6001 agcgattgat gccgaaaaca aagcgttctt ggaagagaat aaaaaagcaa acggtgtaat
     6061 cgttaccgac agcggtttac aatatgaaat tttaaccgaa ggcacaggcg aaaaaccgac
     6121 tgcgacttca accgtgcgtg ttcactacac cggttcatta attgacggca ccgtgtttga
     6181 cagctcggta aaacgcggtc agccggcaga attcccggta aacggagtaa ttcgtggttg
     6241 gacagaagca ttgcaattaa tgccggttgg ctcaaaatgg cgtttaacca ttccacaaga
     6301 attagcctac ggcgaacgtg gtgcagaagc atcaattcca ccatttgcga ccttagtgtt
     6361 tgaagtggaa ttgcttgata ttctctaatc aaactcaaat aaaaaacctg tggaggcatt
     6421 tcacaggttt ttgctattta agataactat attatgcaga aaaaatcact catatctcgc
     6481 ttacttaaaa atattttcct ctacggcagc ctgtttatca ttattagcct ggtggttgat
     6541 tggtatcgct cgccctccgc tccggcacaa tttgagcaaa ccgtgcatta tgatattcaa
     6601 caccagccga aagtaatcgc ccaattaagc cacgagaagc cgatgctact ctatttttgg
     6661 ggaagttggt gccactattg ccaagtggtt tcgccaaata tccaacaatt agcgaatgac
     6721 ggcacggaag ttttaggtgt tgccctgaaa tccggcgatg aaaatgacgt acaagcctat
     6781 ctcaatgaaa atggctacac tttcgctact ttgaatgacc caagcggtga tttctctcga
     6841 ggttggaaca ttcaagccac cccaaccatc ttgattatca aagacggtaa aatcgcccac
     6901 cacaccaccg gctacactag ctatttgagc ttaaaattca gactatggct ggcagacgtg
     6961 ttgtattaaa cgattccttt catttgcaaa aaaatccact aaatccgacc gcttgtttac
     7021 accaaaaaga caagcggtac taaattcatt gtcatcattg tggtgatcga taaaatcagc
     7081 ctcgatgagc caaaaataaa gcctcggttt ttgccgttgt cgaatttcac cagtaaattt
     7141 gccattgctg tgagggcgta cacttccacc accgtaaaaa tcacggtgta aatataggca
     7201 gcggtcagag ttgcgacttt taccgcaata aaccaagggg caataatcgc caccgctaat
     7261 aacggcgaaa tccaaattaa ttgctgtttg gtgaggttga ttttacgaga aaaagtcgat
     7321 tgcaaactga cggttaaaac accgttgatg aataacgcaa tcggcgaatg ctccggtgcg
     7381 tgcaggacat tgtcgaataa atacggaaaa atcactcagc ggcacaaata gcattaagtg
     7441 agcgaacaaa aactctttgt tgatgatttc cttaatttgt gcgaaacgaa atttcacgcc
     7501 gccattcacg ccttccaccg aataaaacgg cttgaccata aacgcaaaca agccggcttc
     7561 caaaataaag cagatccaaa tcagcgtttc aatcatctca aacctaataa acggaataat
     7621 aatcagcggg gcgagcattg aagacatact ggtgactttt agatatttgc cttgtaaacg
     7681 ggttttagca gcatattcat cgccggcaat cgccagtaaa caggctctgg cattagtgcc
     7741 gaataagcaa ctccccaacc caaagcaagt ggttgccaac aataaaatga ggtaattatc
     7801 cgctgctaaa aacagcatat aggcaaccac atctaataaa cagcccaata acatcatttt
     7861 tgccaagccg tagcgatccc cccaaatgcc ggctaaaatt gccaatgcct gattacaaaa
     7921 caacagcaat gaaaggaaaa aagcgatttg ggcgttatct agccctttct cttttaaata
     7981 aaagaaaagg ggctgtccta gataactaac ctaaactacc cttaaccaat tgttttaaaa
     8041 aggatatttg agattttaag tcactgtgat taaaacgcca ttcgcattct ttcaaataca
     8101 gctcaaaatg cactttggga atgccgttaa acttacgcaa atggcgtttt gcttggctcc
     8161 caaaattctc aattccatta atgtgatttt gattttcagc gaagtgtgtg ctgtgattaa
     8221 tgcgaaaatg gttaaattca ctcacatcaa gcacatcata acttcgatag aaatcggtgt
     8281 aaacaatact atcgggtttc accttttccc gaataatcgg caaaagtgtg gcaaattgcg
     8341 tgtttggtac taccacggta taaacctttc cgtcccgctt taaaagcccg aacacggcgg
     8401 ttttgcctgc tgcaccacgt ccacgtttac ctttacgatg accgccgaaa taactctcat
     8461 caacctcaat ttctccctca aacatttcca aatgagggct atgttgatag ataagcaacc
     8521 gcaagcgatg gaagtaatac gccgccgtgt ttttgtttac attgaccaac tcagatgctg
     8581 ttcgggctgt tacccctgca acaaactgct caatgagttt gttttgtttg tactgactta
     8641 gacgactttt tctcatttgg gtatcttaac ctaaatggag tttttagtcg ttatctagga
     8701 cagccccaaa gaaaaacacg ctttgcattg aaaaaaaggc ggtggtggaa ataaaacggc
     8761 gtaggagtaa tagtttttct aaagacattc agaatggttg aattggttaa ataaagagcg
     8821 gttattctac cttttaagtc aaccccaatc aatcggcttt tgttttacct ttggcgtggg
     8881 tttcggtatg ataagcgata tgtagtggcg ggtcctgtgc ccgcctaaat accaatgata
     8941 acttgcgggc gagcacagga ctcgccccta cgataaacaa gaaaatgaaa gacaccattg
     9001 ttgctcaagc aacaccgatt ggcagaggcg gtgtggggat tctgcgagtt tccggtccgc
     9061 ttgccgaaac cgttgctcaa gccgtattag gcaaaacatt gccaccccgt atcgcaaatt
     9121 atttgccgtt taaagacgaa gatggcactg tgttggatca aggcatcgcc ttatttttta
     9181 aagctcccaa ctcctttacc ggcgaagatg tgcttgaact tcaaggacac ggtgggcagg
     9241 ttattttaga tttactgctc aaacgcattt tacaagttaa ggggctacgc attgcccgag
     9301 caggtgaatt ttccgaacag gcatttttaa acgataagct cgaccttgcc caagctgaag
     9361 cgattgcgga tttaatcgac gccaccagcg agcaagccgc tcgttcagcc ctgaaatcac
     9421 tacagggtga attttctaat aaaatcaatg cgttagttga ttcagtaatt tatttacgca
     9481 cttatgtgga agcggcgatt gacttccccg atgaggaaat tgactttctg gcagacggta
     9541 aaatcgaggc aaaattaaat gagattatcg ctcaattaga cggtgttcgc cgtgaagcga
     9601 aacaaggctc gattctgcgt gaaggaatga aagtagtgat cgccggtcgc ccgaatgcag
     9661 ggaaatcaag cttgcttaac gccttagccg gacgagaggc ggcgattgta accaatattg
     9721 ctggcacaac ccgtgatgtg ttgcgtgaac atattcacat tgacggaatg ccgttgcata
     9781 ttatcgacac cgccggtctc cgtgatgcca gtgatgaggt ggaacgtatc ggcattcagc
     9841 gtgcttggga agaaatcgcc caagccgacc acgttttatt aatgattgac agcaccgagc
     9901 aacaagccga tcagttccga caagagtggg cggaatttct agccaaactg ccggcaaata
     9961 tgcctgtgac cgtaattcgc aacaaagtcg atttatccgg cgagcaggaa ggcttaattc
    10021 aggtggacga tttcacgatg attcgcctct ctgcccaaac caaagtcggc gttgatttat
    10081 tgcgtgaaca cttgaagaaa tcaatgggct accaaagttc tacggaaggc ggattcttgg
    10141 ctcgccgccg tcatctgcaa gcattggaaa ccgctgccga gcatttagag cgagggcata
    10201 tccagcttac tcaattctta gccggcgaat tgttagcgga agagttgcgt atggtgcaaa
    10261 atgccttaag tgaaattacc ggccagttca cctctgatga cttattaggc aacattttca
    10321 gctcattttg catcggtaaa taaatggcac tttttaaccg ctttcaaacc cagtgtgcaa
    10381 cgcatttgcc gaaccaaacc gcctttttaa tcggcttaag cggtggggtg gattcggtgg
    10441 tactactcca cctttttgcc cgcacctcat ttcacgttag ggcaatttat gtccaccacg
    10501 ggctaagccc aaatgcggac gactgggcag ccttttgcga acaatattgc aagcggttaa
    10561 atattccctt tattttacaa aaggtcagag tggatagctc aaacggcgtt gaaaacgggg
    10621 cgagagaagc tcgttaccaa gccattcaac aacagcttaa gcccaatgaa gtgttcgcca
    10681 ccgcccacca tttagatgac caagccgaaa cttttttgct cgccctcaag cgaggcagtg
    10741 gagtcaaagg attatcggcg atgcaagcgg tcactttttt gcaaaatttt accgtttttc
    10801 gaccgctact cacttttact aagtcagatt tgatgggtta tgccgttcaa catcagctag
    10861 gttggattga ggacgaaagc aatgccgata accgttacga ccgtaatttc cttcgcaata
    10921 gcatcttgcc actactcaat caacgttggc agcacttcag ccaaatggtt gcccgttccg
    10981 ctcagcattg tgcagaacag caagccttga tagaagagct attaagcaat gaactcaagt
    11041 caagaacagg ggaaaaacaa caactcaaca ttaacggctt tgggcaattt tcacttgcca
    11101 agcaacagca acttagccgc ctttggctgg aacaaaacgg agtgagaatg ccaagccaag
    11161 cccagctaca agcggtcatt tctgaactga tttttgcaaa agccgataaa aacccacagg
    11221 ttaaagtcgg cgaaaatatt atcagacgct atcaacaagc tatttacgtt accgaagaga
    11281 tcgccgacat tccgccgttt gaggtagtac ttcccacaga aaccaaagtg gaattaccct
    11341 atcagctcgg tactattatt cgccaacaac aagagatcat ttgcaaaaaa aacgggaaaa
    11401 atcaccgctt gcaactgccc caagagctgg ctcaatcaac tatttctatt aaaatcgggc
    11461 aactaggcaa agtaaaatgc tacggcaagg ctcaacggga agaaatgaaa aagatttggc
    11521 aaaatcacgg catacctgtt tgggaacgca accgtacacc gctgattttt tggcaagacg
    11581 aattagttgg cctattacct atcctctagg gtgaaattgc tgatgaagcg atttcaaacg
    11641 ggcttttgcc acttgagtgt aaatctgggt ggtcgataaa tcactatgcc ccagcaacat
    11701 ctgtaccact cgcaaatcag ccccatgatt gaccaaatgg gtcgcaaagg cgtggcgaag
    11761 cacgtgaggc gagagtttat ctgcatcaat acctgcaagc acggcatagt gcttaatccg
    11821 atgccaaaac gtctgccttg tcatctgctg acctcgccgg ctcggaaata caacatcaga
    11881 ctgctgatta ttcagcaaaa tagggcgacc gtactggaaa aactcctgta tccaataatt
    11941 cgcctcctcg cccaatggca ctaaccgctc tttatcccct ttaccgataa ttctcaccac
    12001 cccttgtttg aggctgatat tatccatcga aagcgacacc agctccgtta cccgcagacc
    12061 ggtggcatac agcagctcca gcattgcttt atcccgtaac tctatcggat ctaacgtatt
    12121 cggggcatct agcaaatcca ttacctgatc ttcacttaag gatttgggta aagaagcacc
    12181 ccgtttaggg gaagaaaggg ttaaagtcgg gtcatcttgg cgataatgct caaggcataa
    12241 aaaacggaaa aatttcctta aacaactcaa cattctggca gagcttgtcg ccttatagcc
    12301 ttgctccaaa cgctcgccga gaaaaccttg taagtcgata tgatcgagag acagaaatgc
    12361 tttaggttca gaaaaataat cggaaaaacg ctctaagtca gaacgataag aggcaacggt
    12421 attttcagac aaaccgtgtt cttgccatag tgtatcgaga aattgttcaa taatagggtc
    12481 aagctcagcc atacaagcgg gtatattttc aggaaatttt gcaaatttta acagaattaa
    12541 atctataaaa atacaaaagc gatagaaacg aaaaaaccga ttagcttatg ctaatcggtt
    12601 ta
//
LOCUS       DANLXQ010000070        12484 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.070, whole genome shotgun sequence.
ACCESSION   DANLXQ010000070 DANLXQ010000000
VERSION     DANLXQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12484
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.070"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            1..693
                     /gene="fkpA"
                     /locus_tag="PXD94_001477"
     CDS             1..693
                     /gene="fkpA"
                     /locus_tag="PXD94_001477"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6286335.1"
                     /translation="MVGTLFSTNVMAEEKADPKFVDDSSYAVGVLMGKNINGVIESQK
                     DIFTYNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKINEEKTKATVAA
                     GDKFRADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGTLTDGTVFDSSY
                     DRGEPIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGKIPANSTLKFEI
                     ELLDFKAADAKK"
     gene            790..1446
                     /locus_tag="PXD94_001478"
     CDS             790..1446
                     /locus_tag="PXD94_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6286336.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            1446..1823
                     /gene="tusD"
                     /locus_tag="PXD94_001479"
     CDS             1446..1823
                     /gene="tusD"
                     /locus_tag="PXD94_001479"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL6286337.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            1825..2196
                     /gene="tusC"
                     /locus_tag="PXD94_001480"
     CDS             1825..2196
                     /gene="tusC"
                     /locus_tag="PXD94_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL6286338.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            2206..2481
                     /gene="tusB"
                     /locus_tag="PXD94_001481"
     CDS             2206..2481
                     /gene="tusB"
                     /locus_tag="PXD94_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL6286339.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            2597..3496
                     /locus_tag="PXD94_001482"
     CDS             2597..3496
                     /locus_tag="PXD94_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286340.1"
                     /translation="MVMAEENNTWVDKKHETVSTTLDRWANNINSWLGDADPDKPGTA
                     SLRVMLDSRWDKHDKFTYKPRVRGKIKLPILKKHLNVVFGDDELDNELTDKNHAGKLY
                     KNVNPNKRYNSREARDSNSSIGLRWSDGIKALGIETDLDAGIRSGSDIFGRLRISKDW
                     KITDQFSTRLEQIYRYGSESKHYLRTNFENKYTETDTTFIMNHTNFEYRHDVDEERSW
                     GNSLYRQHNFKPLTHISYGISMGGRLDKELSKFNYWGPFVSYRQPIWRNWLFIQPEIS
                     FYNDKDKDRKHFIQTFVRLEAIF"
     gene            3846..7871
                     /gene="rpoB"
                     /locus_tag="PXD94_001483"
     CDS             3846..7871
                     /gene="rpoB"
                     /locus_tag="PXD94_001483"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL6286341.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAAHGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            8134..12417
                     /gene="rpoC"
                     /locus_tag="PXD94_001484"
     CDS             8134..12417
                     /gene="rpoC"
                     /locus_tag="PXD94_001484"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL6286342.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
BASE COUNT         3761 a         2419 c         2881 g         3423 t
ORIGIN      
        1 attgttggca cactttttag tacaaatgtg atggcagaag aaaaagcaga tcctaaattt
       61 gtggatgatt cctcttatgc cgtgggtgta ttaatgggta aaaatattaa tggtgtgatt
      121 gagtcacaaa aggatatttt tacctataac caggataaaa tcttagccgg tgttcaggac
      181 accttaaaga aaaccggtaa attaaccgat gaagatttgc aaaagcagtt aaaatcgtta
      241 gatgcctatt tatctgcgaa agaagcaaag attaacgaag aaaaaaccaa agcaacggta
      301 gctgcaggcg ataaattccg tgctgattat aaaaaacagt ctggcgtaaa agaaacggct
      361 tcaggcctgt tatataaaat tgaaaaagca ggtgaaggca attcaccaaa agcggaagat
      421 actgtgaagg tgcattacaa aggcactttg actgatggca cggtatttga cagctcttac
      481 gaccgtggtg agccgattga atttcagtta aatcaattaa ttccgggttg gattgaagcg
      541 attcctatgt tgaaaaaagg cggtaagatg gaaattgtag tgccacctgc attagcttat
      601 ggtgatcgtg ccgcaggcaa aattccagca aattcaacct tgaaatttga aattgaatta
      661 ttagatttta aagcagcaga tgctaagaaa taattcgtta ttccttaatt attgaagata
      721 cgtaagcggt tagatttatt ataatatttg caaaaaatgt gataaaacta accgcttgta
      781 atttgtatta tgaccaaaaa atttacgcct ttttctgacg aagatcacgc cattcttgct
      841 tcttactttc cgatggtaga tgggattgcg gcactgattg gtgagcattg cgaaattgta
      901 ttgcattcgt tagagtttct ggaacattcg gcaatttata ttgctaacgg acacaatact
      961 aaccgtaaaa tcggctctcc gattactgac cgtgcattga aatcgctgca tcatatggca
     1021 acggatagcg tctccaaacc ttattttacc aaggcaaaag gtggcgtttt aatgaagtct
     1081 atcactattg cgattcgtaa tggtaaacag cacgttatcg ggttactgtg cattaatatt
     1141 aatttggaag tgcctacctc acagtttctc aattctttta ttgcccctgt agataccgct
     1201 tcagaagtta cttttgccag ttcagtagaa gatttggttt cacaaacgat tcaaacgacc
     1261 attgatgatg tgatggcaga tcgtcaaatt tctaacaata ataaaaatcg tcaaattgtg
     1321 atttcgttat ttgagaaagg gatttttgat attaaagatt ccattaacca agtagcggag
     1381 aagctggata tttcacgcca tactgtgtat ctctatattc gtcaaattaa gcaggacaat
     1441 gattaatgga ttatgtatta gcgataaaat caccggttta tggttcacaa ggggcatttt
     1501 tagcctacca agtggcggaa gaattattaa aagccaatca tacaatttgc caaatattct
     1561 tttttcaaga aggcgtgagc aatgctaatg ccttagtgaa tccagcaagc gatgaagtga
     1621 atttagtcga gatgtggaaa aaattggcta aattgcaccg cttgcctctg catttgtgca
     1681 ttgctgctgc ccagcgtaga ggcgtggtgg atgagcaaat ctcagctaat gcaacacata
     1741 atttagccga aggatttacc ctagccgggt taggcgaatt tagccaagca gtattaaaag
     1801 cggatcgatt actgactttt taagatgaag aaatataaac ttgccttttt atttacccag
     1861 ccaccttttg gttcggcagt tagccgtgag gggctagatg ccttgcttgc agctagtgct
     1921 ttttgtgacg agcaagagat agctatcctc tttatcaatg atggcgtatt taatctcatt
     1981 gccgatcaac agccggaaaa actcttacaa aaagatcata tttccacctt caaattattg
     2041 gagttgtatg acttaactga atgttttatt tgccaagaaa gtgtaagttt acgagggctg
     2101 gataatgcag attggttact gcgaatgctt caatttaaaa ctcacgctga gatgatcgca
     2161 ttgctcaatc aagctgaaaa agtaatgacg ttttaaggaa tatttatgct ttataccctt
     2221 tccaaagcga attatgatga agaaacctta aacttctttt tctcacatct tagtgagaac
     2281 gatgcggttg ttctgtggca agatggcgtg ctacaagcgg tcaaaagtcc gcaatttttt
     2341 gcaaaattgc cccgttgcta tttattggca caagatgtgc aagctcgagg attgcaagag
     2401 aagctcactg catttcagtg catttcatta tccgaattag tcaaattaac agcggtttat
     2461 tttccccagg ttgcactgta acctagagag gatatttttt gtaataccaa ataatttaga
     2521 ttatgatgtg attcctaaaa aggagaatat tatgaaaaca aaatgtctta ttccgctact
     2581 agcacttaca cctgttatgg tgatggcaga agaaaataac acttgggtag acaagaaaca
     2641 tgaaacagta agcactacct tagatcgttg ggcaaacaat attaacagtt ggctaggtga
     2701 tgcagaccca gataaaccgg gaacggcttc tttacgggta atgttagata gccgttggga
     2761 taaacacgat aaatttactt ataagccccg tgttcgtggt aaaatcaaac tccctatctt
     2821 gaaaaagcat ctaaatgtcg tatttggtga tgacgaactg gataatgagc ttaccgataa
     2881 aaatcatgct ggaaaattgt ataaaaacgt caatccaaat aaacgctata actctcgtga
     2941 agcgagagat tcgaactctt ctatcggttt acgttggtct gatggcatta aggctttagg
     3001 gattgagacc gatttagatg ctggtattcg ttcgggaagc gatattttcg ggcgtttacg
     3061 catcagtaaa gattggaaaa tcaccgacca gttcagtacc cgcctagagc aaatttatcg
     3121 ttacggttca gagagcaagc actatttacg tactaacttt gaaaataaat ataccgaaac
     3181 ggataccacc tttattatga accacaccaa ttttgaatat cgtcatgatg tagatgaaga
     3241 aagaagttgg gggaatagtt tatatcgtca acataacttc aaaccactca cgcacatcag
     3301 ctatggtatc agtatggggg ggcgtttaga taaagagtta tcaaaattta actattgggg
     3361 tccatttgtc tcctatcgtc aaccaatctg gcgcaactgg ctctttatcc agcctgaaat
     3421 cagtttttat aacgacaaag ataaagatcg taagcacttt attcagacat ttgtacgttt
     3481 agaggctatc ttctagctat taagcctcaa gtagaatctt ggggcttgtt tttgcaagcg
     3541 gtcaatttct tgccaaaatt tacaaaatcc gctataattc ctaaccattt ttagcaaaaa
     3601 ttggctgacg gcttgaccgg tcagcctttt gtgcttgaac gcaagggctg attttgtcat
     3661 tttaatcagc aaaatttgca aatttcctgc ccaatttgac cgcttgcagt agcgatttct
     3721 gactgcttga ttggcgattg gaaaatgaca tctcgtacag tttttataat gccacttcca
     3781 accgagtctt tcgtgtcgga ttgtgtaatc tcaaccaaaa tatatttcag aggctatctt
     3841 aaaaaatggc atattcatat tccgagaaaa agcgtatccg taagagcttt ggcaaacgcc
     3901 cacaagttct gaacgtacct tatctattaa caattcagct tgattctttt gaaaaattta
     3961 tcgaaaaaga tcctgaaggg cagcaaggtt tagaagcggc attccgttct gttttcccga
     4021 ttgtgagcaa caatggtgca acagaattac aatacgtttc ttacgaatta ggcgagccgg
     4081 tgtttgacgt gcgtgagtgc caaattcgtg gtacaaccta cgctgcacca ttacgcgtga
     4141 aattgcgttt agtctcttac gatcgtgaag ccgcagccgg tacggtaaaa gacattaaag
     4201 agcaaaacgt gtatatgggc gaaatcccat taatgaccga taacggtact ttcgtgatta
     4261 acggcaccga gcgtgttatc gtttctcaat tacaccgtag cccaggtgtg ttcttcgatt
     4321 ctgacaaagg taaaacccac tcttcgggta aagtgcttta taacgcacgc attatccctt
     4381 accgtggttc ttggttagat ttcgagtttg atccgaaaga taacttattt gcccgtatcg
     4441 accgccgtcg taaattgccg gcaaccatta ttctacgtgc attaggctac accaccgaag
     4501 aaatcttaaa tttattcttc gagaaaatta aatttgaaat tgccgataat aaattattga
     4561 tgacgttagt gcctgagcgt ttaagaggcg agactgcatc atttgatatt gcagcaaacg
     4621 gcaaagtata tgttgaaatg ggtcgccgta ttaccgctcg tcatatccgt gcgttagaaa
     4681 aagacaatgt gacccaaatt gaagtgccgg tggaatatat tgtcggcaaa gtagcggctc
     4741 aagattacgt tgatttatct tcaggtgaag tgatttgccc ggcgaatatg gaaatctcga
     4801 tggagatgtt ggcgaattta tcacaagccg gttacaacca gatcgaagta ttatttacca
     4861 acgatttaga ccacggtcca tatatttctg aaaccttacg tgttgatcca acttacgacc
     4921 gtttaagtgc gttggttgaa atctatcgta tgatgcgtcc gggcgaacca ccaacgaaag
     4981 aagcggcaga agcattattc gataatatgt tcttctcggc agagcgttat gatttatcgg
     5041 cagtaggtcg tatgaaattc aatcgctcgt tagaaattcc ggaaggcgta ggcagcggta
     5101 ttttaagcaa cgatgacatc atcggtgtga tgaaaaaatt aatcgaaatc cgtaacggtc
     5161 gtggtgaagt ggatgatatc gaccacttag gtaaccgtcg tatccgcagc gttggtgaaa
     5221 tggcagaaaa ccaattccgc attggtttgg tgcgtgtaga acgtgcggtg cgtgagcgtt
     5281 tatctttagg cgatttagat ggtgtaacac cacaagattt aatcaacgct aaaccgattt
     5341 cagcagcagt gaaagagttc tttggttcat cacaactttc gcaatttatg gaccaaaaca
     5401 acccgctttc agaggtaaca cataaacgcc gtatctcggc attaggcccg ggtggtttaa
     5461 cccgtgagcg tgccggcttt gaagtgcgtg acgtacaccc gactcactat ggtcgtttat
     5521 gtccaatcga aactcctgaa ggcccgaata tcggtttgat caactcacta tcggtgtatg
     5581 ctcgtactaa taactacggt ttcttagaga ctccattccg taaagtggtg aatggtcaag
     5641 taaccgaaga gatcgagtat ttatctgcga ttgaagaaag caacaatatc attgcacagg
     5701 cgaactcaaa cttagatgag aacttccgtt ttaccgatac ttatgtaact tgccgtcagc
     5761 aaggcgaatc gggcttattc aaaccggaag aagttcaata tatggacatc tcaacccaac
     5821 aagtggtttc tgtggctgcg gcgttaattc cgttccttga gcacgacgat gcgaaccgtg
     5881 cgttaatggg tgcgaacatg caacgtcagg cagttccaac cttgcgtgcg gacaaaccat
     5941 tagtcggtac cggtatggaa aaaccaattg ctcttgactc aggtgtagcg attgtcgcaa
     6001 aacgtggtgg tacggtgcag tatgttgatg cgtcacgcat tgtagtgaaa gtaaatgaag
     6061 atgaaaccat tccgggcgaa gcgggtatcg atatttataa cttaatcaaa tacacccgtt
     6121 caaaccaaaa cacctgtatc aaccaagtac cttgtgtgaa cttgggtgag ccggttgctc
     6181 gtggtgaaat cttagcggac ggtccttcaa cagacttagg tgaattggca ttaggtcaaa
     6241 atatccgtgt ggcatttatg ccttggaacg gttataactt cgaagactca atgttagttt
     6301 ctgagcgtgt ggtacaagaa gatcgcttta ccactatcca cattcaagaa ttatcttgtg
     6361 tggcgcgtga taccaaatta ggtgcagaag agatcaccgc tgatattcca aacgtgggtg
     6421 aatctgcatt aagcaaatta gatgaatcag gtatcgttta catcggtgca gaagtgaaag
     6481 gtggcgacat cttagtaggt aaagtaactc ctaaaggtga aacccaatta acaccggaag
     6541 aaaaactatt gcgtgcgatc ttcggtgaaa aagcctctga tgtgaaagat tcatcattgc
     6601 gtgttccaaa cggtacttca ggtacggtta tcgatgttca agtatttacc cgtgatggcg
     6661 tagagaaaga taaacgtgcg aaagaaatcg aagagatgca actgtgtgaa gcgaagaaag
     6721 acttagcgga agagttggaa atcttagaag ccggtttatt tactcgtgtt cgcaacctat
     6781 taattgaagg cggcgtaaaa gaatctgcat tagatggcgt tgctcgtgaa aaatggttag
     6841 aacaaaccct tgaagacgaa gcgaagcaaa atcagttaga acagttagcg gaacagcacg
     6901 aagagttacg caaagaattt gaacgtaaac ttgagatcaa acgcaacaaa attatccaag
     6961 gcgacgattt agcaccgggt gtgttaaaag tagtgaaagt ttaccttgcg gtgaaacgcc
     7021 aaatccagcc gggtgataag atggcgggtc gtcacggtaa caaaggggtt atctcgaaaa
     7081 tcaaccctgt ggaagatatg ccatacgatg aaaacggtca gccggttgaa atcgtattga
     7141 acccgctggg cgtaccgtct cgtatgaaca tcggtcagat cttagaaacc cacttaggct
     7201 tagcggctca tggtatcggt gatcagattg ataagatgat caaacagcaa caagaagtgg
     7261 ctaaattacg tgaatatatg caaaaagcct acgatttagg tcacggctca caagtcgttg
     7321 atttaagcac tttctctgat gaagaagtga tgcgtttagc gcaaaactta cgcaaaggct
     7381 taccgcttgc tactccggta ttcgatggtg cacacgaaag cgaaatcaaa ggcttattag
     7441 agttaggcgg cttaccgact tcaggtcaga tcacacttta tgatggtcgt accggtgaga
     7501 aatttgagcg tccggtaacc gttggttata tgtatatgct caaattgaac cacttggttg
     7561 atgataaaat gcacgctcgt tcaaccggtt cttatagcct tgttactcaa caaccattgg
     7621 gtggtaaagc tcaattcggt ggtcagcgtt tcggtgagat ggaggtttgg gcattagaag
     7681 catacggtgc ggcttatact cttcaagaaa tgcttacggt gaaatcggac gatgtgaacg
     7741 gtcgtacgaa gatgtataaa aacattgtag atggtacaca ttatatggag ccgggtatcc
     7801 ctgaatcctt taacgtaatt accaaagaga tccgtgcatt agcaattgat atggagctgg
     7861 acgaagcgta atggtttcac tctccccctt gatgggggag agacaggcaa aggatttgtt
     7921 cagaatcctt tggcagagag agggtgatgc aagcggtgta aatttgctaa atttttgcaa
     7981 aaatcgaccg cttaccctct ccctgatttt gcttctgaac gaggcaaaat ctatccctct
     8041 cccacaaggg gagagggttt aaaatcgcaa ccaaattcgt agagtggata ttctttccac
     8101 caaacaaaac aacctcaaac taggggcaaa aaagtgaaag acttagtgaa gtttttaaaa
     8161 gcacaaacaa aatcgaacga agattttgat gtgattaaaa ttggtttagc atcaccggac
     8221 aagatccgtt cttggtcttt tggtgaagtg aaaaaaccgg aaaccattaa ctatcgtacc
     8281 ttcaaaccgg agcgtgatgg tttattctgt gcacgtattt tcgggccggt aaaagattac
     8341 gagtgcttgt gtgggaaata caaacgttta aaacaccgtg gcgtaatttg tgaaaaatgt
     8401 ggtgttgaag taacccaaac taaagtacgt cgtgagcgta tggggcacat tgaattagct
     8461 tgccctgtcg cacacatctg gttcttaaaa tcactaccgt cccgtatcgg tttaatctta
     8521 gatatgccgt tgcgtgatat tgaacgtgtg ctttattttg aatcttatgt agttactgaa
     8581 ccgggaatga ccgatttaga gaaaaatcag ttattaaccg aagaacaatt tatggacgct
     8641 gaagagcgtt ggggtgatga atttgaagcg aaaatgggtg cagaaggtat ccaagcatta
     8701 cttcgtgatt tagatttaga acaccaatgc gaaaccttgc gtgaagagtt acaagaaacc
     8761 aattcagaaa ccaagcgtaa aaaaatcact aagcgtttaa aaaccttaga agcattctta
     8821 caatccggca acaaaccgga atggatggtg atgacagtat taccggtact tccaccggat
     8881 ttacgtccgt tagtaccgct tgacggtgga cgttttgcga cttctgactt aaacgattta
     8941 taccgccgtg taattaaccg taacaaccgt ttaaaacgct tgttagattt agttgcaccg
     9001 gacatcatcg tgcgtaacga aaaacgtatg ttacaagagt cggttgatgc gttattagac
     9061 aacggtcgcc gtggtcgtgc gattaccggt tcgaacaaac gtccgttaaa atctttggca
     9121 gatatgatca aaggtaaaca aggtcgtttc cgtcaaaact tattaggtaa acgtgtagat
     9181 tactcaggtc gttcggtaat taccgtaggc ccatacttac gcttacacca atgtggttta
     9241 ccgaagaaaa tggcgttgga attattccgt ccgtttatct actcaaaatt agaaaaccgt
     9301 ggcattgctt caactattaa agccgcgaag aaaatggtag agcgtgaaga tcctatcgta
     9361 tgggatattc ttgccgaagt gattcgtgaa cacccgattc tacttaaccg tgcaccaaca
     9421 cttcaccgtt taggtatcca agcgtttgag ccgttattga ttgaaggtaa agcgatccag
     9481 ttacacccac tcgtgtgtgc ggcgttcaat gcggacttcg acggtgacca aatggcggtt
     9541 cacgttccat taacccttga agcacagtta gaagctcgtg cgttaatgat gtcaaccaac
     9601 aacgtacttt caccggcaag tggtgatccg attatcgtac cgtctcagga cgttgtactc
     9661 ggtctttact atatgacccg tgagaaagta aacggtaaag gtgagggaat gtacttcctt
     9721 gaccctcgtg aagcggaaaa agcataccgt accggtcagg ctgaattaca ttctcgtgtt
     9781 aaagtgcgtg ttactgaata tgagaaaaac gaagcaggcg aattagaagc aaaaacttcg
     9841 ttagtagata ccaccattgg tcgttcaatc ttatggatga ttgcaccaaa aggtatgccg
     9901 tttagcttgt tcaaccaaac cttaggcaaa aaagcgatct cgaaattaat taacgagagc
     9961 tatcgccgtt taggtttaaa agaatctgtg gttttagctg accaaattat gtacaccggc
    10021 tttgcttatg ctgctcgttc tggtgcctca gtaggtatcg acgatatggt aattccggag
    10081 caaaaatacg caatcattga agcggctgaa gcggaagtcg ctgagatcca agagcaattc
    10141 aacggcggtt tagtaacagc cggtgagcgt tataacaaag tgatcgatat ttgggctgct
    10201 gcaaacgaac gtgttgcgaa agcaatgatg gaaaaccttt caacggaaga agtaatcaac
    10261 cgtgaaggta atccggaaaa acaagcctca tttaacagta tctttatgat ggcggactcc
    10321 ggtgctcgtg gttctgctgc acagattcgt cagttagcgg gtatgcgtgg cttgatggct
    10381 cgtccggacg gttcgattat cgaaacccca attactgcaa acttccgtga aggtttgaac
    10441 gtattacagt actttatttc aacccacggt gcacgtaaag gtcttgccga tacggcatta
    10501 aaaacagcga actccggtta cttaacccgt cgtttagttg atgtggcaca agatttagtt
    10561 atcattgaag atgactgtgg tacgcacgaa ggtatcgtga tgacaccact tatcgaaggt
    10621 ggtgatgtga aagaagccct gcgtgatcgt gtattaggtc gtgtggtggc agaagatgtg
    10681 ttaaaaccgg gtacagatga agtattaatt ccacgcaaca ccttattaga tgaaaaatgg
    10741 tgtgatgtgg tggatcgtga atctgtggat gtgatcaaag tacgttcggt agtaacttgt
    10801 gatacagact tcggtgtgtg tgctaaatgt tacggacgtg acttggctcg tggtcactta
    10861 atcaaccaag gtgaagcggt cggtgttatt gcggcacaat ctatcggtga gccgggtaca
    10921 cagttaacga tgcgtacgtt ccatatcggt ggtgcggctt ctgcggcagc aaaagaatct
    10981 agcatccaag tgaaaaacgc aggtacgatt aaattaacca acgctaaatt tgtaaccaac
    11041 aaagaaggca aaatcgtatt aacttctcgt aatactgaat taactgtcat cgacaccttc
    11101 ggtcgtacca aagaaaacta taaagtgcct tatggtgcgg tgctttctaa acacgacggt
    11161 gcagaagtgg ctgtgggtga agtggttgcg aactgggatc cgcacacaat gccggtaatc
    11221 tcagaggtat caggtcgtat ccaatttagc gacatcgtag atggcttaac cgttactcgc
    11281 caaaccgatg aattaaccgg tttatcatct atcgtggtgc aagatgtggg tgaacgtgca
    11341 accgcaggta aagatttacg tccggcatta cgcttagttg atagcaacgg caaagacatc
    11401 ttaattccgg gtacagacgt tgcagcacaa tacttcttac cgggtaaagc aatcgtaacc
    11461 ttagacgatg gtgcagaaat tgaagtcggt gaagccttag cccgtattcc acaagaatct
    11521 gtggggacga aagatattac cggtggtctt ccacgcgtag cagacttatt cgaagcccgt
    11581 aaaccgaaag agccggcaat cttggcggaa atttcaggta ttgtgtcgtt cggtaaagaa
    11641 accaaaggta aacgccgctt ggtaattact ccggcggaag gcgaagcgtt tgaagagatg
    11701 attccaaaat ggcgtcagct caacgtattt gaaggcgaga tggtacaacg tggtgatgtg
    11761 atatcagacg gtgcagaaac tccgcacgac atcttacgct tacgtggcgt tcacgccgtt
    11821 accgactaca tcgtaaacga agtgcaagaa gtttaccgct tacaaggggt aaaaattaac
    11881 gacaagcaca tcgaagtgat tgttcgccaa atgttgcgta aagctgtgat caccaacgct
    11941 tacgactctg aattcttaga gggcgaacaa gtggaagtgg ctcgtgtgaa aattgccaac
    12001 cgcaaacgtg ctgaagaagg caaaccgttg gtagaatttg aacgtgaatt gcttggtatt
    12061 accaaagcat ctcttgcaac agaaagcttt atttctgcag cctcgttcca agaaaccact
    12121 cgagtgctta ccgaagccgc agtggcaggt aaacaagatg aattgcgtgg cttaaaagag
    12181 aacgtaatcg ttggtcgctt aatcccggca ggtaccggtt ttgcttacca ccaagcacga
    12241 gctaaaaaac gtgcccaact ggaagcagga attgcagttg tccctgaatt tgaagcaccg
    12301 gcagctaaag ccaattcatt cgttaccgat gcagacatcg aagctgaatt tgaatttgtt
    12361 gcagacgatg caacccaaag tttagcagcg ttgttaaatg cgggtgagga agagtaatag
    12421 ctcgtagggt atgataaatt aaaaaatctc ctagaatata atttctagga ggttttctac
    12481 ttga
//
LOCUS       DANLXQ010000071        12452 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.071, whole genome shotgun sequence.
ACCESSION   DANLXQ010000071 DANLXQ010000000
VERSION     DANLXQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.071"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            137..796
                     /locus_tag="PXD94_001485"
     CDS             137..796
                     /locus_tag="PXD94_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL6286343.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            complement(856..2181)
                     /locus_tag="PXD94_001486"
     CDS             complement(856..2181)
                     /locus_tag="PXD94_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL6286344.1"
                     /translation="MLIFNLIVFAAILFLLAKIYKSTQKLGQTVFIGLFLGLASGAFL
                     QTFYEKATVDATLDWINLIGNGYVRLLQMIVMPLVFVSILSAITRLKEAGSLGKISFS
                     VLSVLLVTTAIAAAIGIALVYLFDLSAEGLVAGSRELAAQEKVVGRVEQVSNLSVPSM
                     LLSFIPKNPFLELTGANPTSIINTVIFSALLGIAALSLGKEDKALGERIAQGVETLNK
                     LVMRLVRFVIRLTPYGVFALMIKMAATSQWSDIVNLGSFIVASYIAIGLMFIVHGILL
                     FVAKINPLDYYKKVLPTLSFAFTSRSSAATIPLNIETQTNKLGNNSVIANFSATFGAT
                     IGQNGCAGIYPAMLAVMVAPTVGMDPFSLNYILTLILVVAISSFGIAGVGGGATFAAI
                     VVLSTLNLPIELVGLLISVEPIIDMARTALNVNGAMVAGTLTNKWVKAE"
     gene            complement(2282..3034)
                     /locus_tag="PXD94_001487"
     CDS             complement(2282..3034)
                     /locus_tag="PXD94_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL6286345.1"
                     /translation="MYQAIVHFSYHNFEQDPVTAISQILNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVIFASFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NLLECHALVQISDETSDLAMIGRDLCQQIEQKTHIPTFYYLYRLGRDESEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCEPCRLISNLSWELL"
     gene            complement(3034..3939)
                     /locus_tag="PXD94_001488"
     CDS             complement(3034..3939)
                     /locus_tag="PXD94_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL6286346.1"
                     /translation="MNEKYSKLVKNAANLAIIVAILLIVIKAFAWWKTGSISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSAVFLLLQGF
                     HKLTNPELIAQSSIGVIVSIVSIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGFVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIERI
                     KEIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFQDMAENI"
     gene            complement(3948..4511)
                     /locus_tag="PXD94_001489"
     CDS             complement(3948..4511)
                     /locus_tag="PXD94_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL6286347.1"
                     /translation="MSAIKKGITLVALSLAGCVSQSPQFAVPDAIHFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNPENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVEIQQNELHSSVIYPPIERFNNVMLEVTRGRNLACGYGQIQFADKREVGKTVAKKSP
                     NLTAYSQEIARLSLELNQLGWQVQCSK"
     gene            complement(4511..5287)
                     /gene="yaaA"
                     /locus_tag="PXD94_001490"
     CDS             complement(4511..5287)
                     /gene="yaaA"
                     /locus_tag="PXD94_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL6286348.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     ELGSLMSISDKLAALNVARFAEWQLEHNEQNAKAALFAFKGDVYTGLEAETLSKAQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGKDLYAFWGNIITENLQQAID
                     EQGDDILVNLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     ILDTQPTSIEQLKQFNYGGYWFDADSSSESELVFKREEQA"
     gene            complement(5476..7196)
                     /gene="ltrA"
                     /locus_tag="PXD94_001491"
                     /pseudo
     CDS             complement(5476..7196)
                     /gene="ltrA"
                     /locus_tag="PXD94_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341372.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(8878..9273)
                     /gene="rpsI"
                     /locus_tag="PXD94_001492"
     CDS             complement(8878..9273)
                     /gene="rpsI"
                     /locus_tag="PXD94_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL6286349.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            complement(9290..9718)
                     /gene="rplM"
                     /locus_tag="PXD94_001493"
     CDS             complement(9290..9718)
                     /gene="rplM"
                     /locus_tag="PXD94_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL6286350.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
     gene            10017..10412
                     /locus_tag="PXD94_001494"
     CDS             10017..10412
                     /locus_tag="PXD94_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL6286351.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
     gene            complement(10573..12144)
                     /gene="guaA"
                     /locus_tag="PXD94_001495"
     CDS             complement(10573..12144)
                     /gene="guaA"
                     /locus_tag="PXD94_001495"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL6286352.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVALKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
     gene            complement(12161..12448)
                     /locus_tag="PXD94_001496"
     CDS             complement(12161..12448)
                     /locus_tag="PXD94_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286353.1"
                     /translation="MVALSACSIFGPSYSGETTASPLLKLDTERNINLYFQAIHKCTP
                     DKIHTKIINIPSEGNAKELWTATGCNKTQSFIITYRNDGVGGTFINMTTTP"
BASE COUNT         3549 a         2788 c         2358 g         3757 t
ORIGIN      
        1 atttttaagg taaagcaatt ttaaacaagc ggtcattttt tcctaatttt ttgcaaatta
       61 acaggcgtga aattcgcatt ctgctctaaa tcaagttatc attaatctac ttatttcact
      121 tataaaagga aaaacgatgt ttaatattag caaacagcaa gttttagacg cttttcactt
      181 ccgttcggcc tgtcgcagtt acgatccaaa caaaaaaatc aacaaagaag atatggatta
      241 tattttggaa ctcgggcgtc tctcaccaag ctctgtcggt tctgaaccgt ggaaattttt
      301 agtgctacaa aacccggaaa cccgtcagaa aatcgccccg gtttcgtggg gaattaaaca
      361 cccaatggaa gaaatgagcc atttagtggt gattttaacc aaaaagaatg tccgttatga
      421 ttctgtctat atgcgtgata tgttagccaa acgaggctta acctatgagc agattgaagc
      481 ctcgctggta cgctataagt cgttccaaga agaggatatg aaattgctta gtgaacgtga
      541 attattcgat tggagtacca agcaaaccta tattgcatta ggcaatatga tgaccggtgc
      601 ggctctgatt ggtattgact cttgcccgat tgaaggtttt cattacgata ccgtaaacca
      661 aattttatcg gacgaaggct tatttgacct aaacgaatat ggtgtgtctt gtatgataac
      721 ttttggctat cgcaacaaag aaattaagaa aaaatcccgc aaacctaccg aagaagtgat
      781 tgcgtggatt gaataatcac cttaacttat aaaaacgggc gaattaatcg cccattttta
      841 tatctttagt tttgactact cggctttcac ccatttattg gttaatgtgc ccgccaccat
      901 tgcaccattc acgtttaagg cggtgcgagc catatcaata atcggctcaa ctgaaatgag
      961 caagcctact aactcaatcg gcagattcaa ggttgatagc accacaatcg ccgcaaaagt
     1021 tgcaccacca cctacaccgg caatgccaaa tgaggaaatt gccaccacca aaatcaaggt
     1081 taaaatatag tttaagctaa acggatccat tcccactgtt ggagcgacca tcaccgccaa
     1141 cattgccgga taaattcccg cacagccatt ttgcccgatg gttgccccaa aggttgcaga
     1201 aaagttggca atcacactgt tattacctaa tttattggtt tgcgtctcaa tattcaacgg
     1261 aatggttgcc gcactggaac gagacgtaaa agcaaaactt aatgtcggca acactttttt
     1321 gtagtaatcc aacgggttga ttttagccac aaagagtaaa ataccgtgca caataaacat
     1381 caaaccaatc gcaatgtatg atgccacaat aaagctacct aaatttacaa tatcgctcca
     1441 ttgagaagtt gccgccattt tgatcatcaa cgcaaatacg ccataagggg ttaggcgaat
     1501 cacaaaacgt actaaacgca taaccagttt atttaaggtc tctacacctt gtgcgatacg
     1561 ttcgcccaac gctttatctt ctttgcctaa gctcagtgca gcaatgccta gcagagccga
     1621 gaaaataacc gtgttgatga tcgacgttgg gtttgcaccg gttaattcta aaaacggatt
     1681 tttcggaatg aaagaaagca acattgaagg cacagataag ttgctcacct gctccacacg
     1741 ccctaccact ttctcttgag cggcaagctc acgggagccg gcgactaaac cctcggcaga
     1801 taaatcaaac agatacacta aagcaatacc aatcgctgcg gcaatcgcgg tggtcactaa
     1861 taacactgat agcacactaa aactaatttt gcctaaagat cctgcctctt ttaagcgggt
     1921 aattgccgat aaaatagaga caaacaccaa tggcattacg atcatttgta gcaagcgaac
     1981 ataaccgtta ccaattaagt taatccaatc aagagttgca tcaacagtag ctttttcgta
     2041 aaaagtttgc aaaaaagcac cgcttgcaag ccctaaaaat aacccaataa acactgtttg
     2101 accgagtttt tgcgtagatt tataaatttt agccaataaa aataatattg ccgcaaagac
     2161 aattaagttg aaaataagca cagaacctcc aaagtaaaac cgcttattat aaaaatgccg
     2221 caagggtatt acgcttgcgg cattcattca agatgattta ttactttggt ataactaaaa
     2281 gttataataa ttcccagctt agattagaga tcaaacggca aggctcacat ctaaaatgga
     2341 aaatatcgtg caacggctca gttaatttcc attctccgcc acaacaaggg catttgcgtt
     2401 gatattcact ttcatcacgc cctaaacggt agaggtaata aaaggtggga atgtgagttt
     2461 tctgctcaat ttgctgacat agatctcgac cgatcattgc aagatcgctt gtttcatcac
     2521 taatttgaac aagtgcgtgg cactccagca gattgccgtt catttgtagc agatcgcacg
     2581 cttgccaatt ttgctgccat tccaatatcg cttcgctaag agactgatcc tgtaaagaat
     2641 aaagcggaat tggggcaaac tgctctccat cataaagtgg cgagcaggta tctaaatggg
     2701 tggtatataa tatttgaaac gctggcgagc ggttatgact gctttcttcg ccctgataat
     2761 ctcgcccgac aagctcaaaa cttgcaaaaa ttacgccgat ttcgaccgct tgttgcaacg
     2821 cttcatttac ttcatcacta ttatattgtg gcaaaaggct ttcttgtgta ggcacacaaa
     2881 cccgagcttg aaactcgttt tgatgaaaag taatcggcat ttctctgcca atgacttgcc
     2941 cgttatataa ccattgattt aagatttggc taattgccgt caccggatct tgctcaaaat
     3001 tatgatagct gaaatgcact atcgcctgat acattaaata ttctccgcca tatcttgaaa
     3061 ttcttgtttg accaccgtag tcggctcttg atgaataatc acgtgagaca tcggaaatgc
     3121 cgccagcaat ttctgctcaa ggctatcagt tatttcgtga gctactagca gcgttaagtg
     3181 atcgtctaat tccaaatgta attggataaa acgcactgca cctgcacggc gggttagaat
     3241 atcgtgaatc cctaaaatgt tcggatgttg cgaagcaatc tctttaatcc gctcaatttc
     3301 ttcatcgggc aaagcttggt ctagcaaggt ttgcaccgca caccaaaaca tttttactgc
     3361 attgaaagca atgtagaggg caatacctat tgcaaaaact gcatcggcgt acacaaaacc
     3421 ggcactatta agcaacattg ccactaaaat tgccgcattc attaataaat cagtttggta
     3481 atgtaacgaa tccgcttcaa tcgccggact tttcgtaagc gtgacaacat aacgctgata
     3541 aaagactaaa agtgcagtaa tcacaattga tacaatactg acaattaccc caatactcga
     3601 ttgagcaatt aattccggat tggtgagttt atggaaacct tgtagcagta ggaaaaccgc
     3661 cgagccggaa ataaacgcac tttgggcaag tgccgccaaa gactccgctt tgccgtgccc
     3721 aaacgcgtga ttctcatctg ccggttgtag tgcaaaacgc agcactaaaa tattggttag
     3781 cgaagcaaac agatccacta ccgaatccgt cattgctgcc aagattgaaa tcgaaccggt
     3841 tttccaccat gcaaaggctt taatcacaat cagtaagatc gcaacaataa tcgctaaatt
     3901 cgctgcattt ttgactaatt tgctgtattt ttcgttcatt tactacatta tttactgcat
     3961 tggacttgcc aacccagttg gtttaattca agagaaagtc tagcaatttc ttggctgtaa
     4021 gcggtcaaat tcggcgattt ttttgcaact gtcttgccaa cttcccgctt atctgcaaat
     4081 tgaatctgcc cgtaaccaca agctaaattc cgcccacggg ttacttctaa catcacattg
     4141 ttaaaacgtt ctataggtgg ataaatcaca ctgctgtgta attcattttg ctgaatttcc
     4201 actttcgcct caagctccgg ctttttcgca aaacttgctt ggcggaatgc agctcgttgc
     4261 tctaaggttt gatttttact gtttttatct aagaaaaata aaatgccttt ttgccaattt
     4321 tccggatttt tctcactctt ttctggcaaa taaagtaaat gttgcatttc atcaatttgg
     4381 ctgtgggtga cttttacaaa agtatcctgc ccaaagtgaa tagcatcagg cactgcaaat
     4441 tgtgggcttt ggctgacaca acctgccaat gataacgcaa ccaaagttat tccctttttg
     4501 atagccgaca ttatgcctgc tcctcacgtt taaagactag ctcgctttca ctagaactgt
     4561 ctgcatcaaa ccaatagccc ccgtagttaa actgtttgag ctgttcgatg ctagtcggtt
     4621 gggtgtctaa tataaagcga accatcattc cacgggcttt tttggcatag aagctgatca
     4681 ctttatattt accgtttttc tcatctaaaa acaccggctt gataatagtc gcatttagat
     4741 tttgtggctt aaccgcacca taatattcat cagaggcaag attaactaaa atatcatcgc
     4801 cttgttcatc aatcgcttgc tgcaaatttt cagtaataat attgccccag aaagcataaa
     4861 gatctttgcc tttcggattg gctaacttag tgcccatttc taaacgataa ggctgcatta
     4921 aatctaacgg ctttaataaa ccatacaagc ctgaaagcat acgcaaatgt tgttgagcat
     4981 attcaacttg tgctttgctt aacgtttctg cctctagtcc ggtataaaca tcacctttaa
     5041 aggcaaagag ggcggctttg gcattttgtt cgttatgctc aagctgccac tcggcaaagc
     5101 gagccacatt taaagcggct aatttatcgc taattgacat caaactacct aattcagcag
     5161 gtgaaagctg tttacaaata tcaattaatt cttggctaca agcggtcaaa tttggttgag
     5221 aaaatgcaaa accgtcaata ttggttttaa aatcaagggt tttggcaggg gaaataatgg
     5281 ctaacataaa tgcctctatc aaatagttaa tcagggcggt tattctacca cagtcataaa
     5341 gacaaaaaag gcaggaaacc ctgccttctt gaaaacgaaa agaaaagtcg ggtagcgaaa
     5401 gtacattact gcacctccgc cccctaagaa ctgtacaagc aaatttctct gcatacagct
     5461 caagcctttc atcagctata tagccggctt cacgacctcc aagttcaaag tatgaatctt
     5521 tatgtgacaa gtcggatgta ccaaacaacg attgcttaat gcatcgcttc ctcctaacat
     5581 tttgtacacg atatgatgat cgtgccagcc tgtttctttg gttattggca acccacataa
     5641 tgcacatttt ccacgctggt ctttatacag tgcctgccat tgtcgtctgt gtgattgttc
     5701 ttcatacaga cgttcttggc tcagtttttc gccgtacaac tcccattcag gcaaaaacgg
     5761 attatattca gacttaattt taacgtgtcg tttgatttta acatagccac aatagaataa
     5821 gtctatcggc ttagtttctc ctttttcatt ggtataaact gtgccaaata cccaatttcg
     5881 agtcgctgtg gtacaaaagt atttctcttt tatccacata atacctttat tgtgatgacg
     5941 gcgttcacac catttccaaa gtactttcca aactaatgcg tccattcgtc cgaatgcctt
     6001 acttgcaact tggcttcgat gataatttgc ccaacctcgt aacattgggt ttagcaccat
     6061 tataagatct tcctgtttag ccattttcat ttagaaatta cctcttttac cttgttgtag
     6121 aacgctttca cattcttttg acttggtctg ttcagtattt ttcctttgaa acgtctcaca
     6181 ttccaaccta gaaaatcaaa accttcttca atatgtacca ctttcgtctt ttctgttgat
     6241 aaggataaac ctcgttcatt cagaaagttc tggataagtg ggataacttg attttcaagt
     6301 aactctttcg ttttacctga tatgataaag tcatctgcat aacgtactaa gtaagtttta
     6361 ttttctcgaa ttgtccgact gccttttcct ccaaaatgag tttctaacag tttttccaat
     6421 ccatctaatg ccatatttgc aagcgttggc gagataatcc caccctgtgg tgtaccttcc
     6481 tctgttcgtt tcaaccgtcc aaattccact acacctgatt ttagccattt ccgcaatatc
     6541 cgtttattca ttggaatatg ctttaatagc cagtcgtggc ttatgtggtc aaaacaacct
     6601 gcaatatccg catcaagtac ccactcaaca ggttgattag ttttcttgcc tttatgcgaa
     6661 taaatttagt gtatatgagt aatagcatct accgttgaac ggtttaatct aaatccataa
     6721 gaacctttat ccgcagttgt ttctgccaca ggttgtattg ccaataaata aagggcttgc
     6781 attgctctat ctcgcattgt tggaataccc aacggacgtt tctttccatt cggtttgggg
     6841 atatacactc gtcttaatgg actaggttta tacccttttt ccgtcaatga tagtgcggct
     6901 tgccattttg tctctggggt ctcccaaagc tctttatcga caccacttgt tcgcttacct
     6961 tgattttcag tcactcttct tactgcaact aatttagcat aatgtgagtg ggttagcatt
     7021 cgttgtaagt ttttaacctt acgccaattg ccttccagtg ttgccttcgc aatccgaatt
     7081 tgcattcctt tcacatacgt ttcagcttta tgccaatcga cataatgcca tttggagatg
     7141 tgcgaggtcg ctggattgca agctaacttt tcagtataat gcttacgctc tttcatacct
     7201 acctctgata ttacaagata agtcgaaagt ctcagcaagt gggtagatcc ttcaagtaaa
     7261 caaatcccta ttgagataaa ctatccttag tttattccct ttggttacaa gaggatctta
     7321 ccgttttacc gataatttca accgctcttg taaccgattg ggttaaagag tcgagcgcta
     7381 ggttatgatg agcctaacgc tcatttcttt aatttgtttt tgtacctatt caacattgta
     7441 ttccttgata acattcaatt tctcttctgt tatctacaat ctctttcggt gttaccctta
     7501 aaggaaacgc tattcatctt acgatgaaag accagttaga agtctgcacc ctttcgggtt
     7561 aggctatgtt tcacacccta tctatgccat tacagcatag cattcgcttt ttctaacctc
     7621 ccttacctgc attcccaaca gtatcgctta cgctttactt gccttttcct cttttacctt
     7681 cgccatctat ttcaaacggt ttgagtattg tgggcaaggc agaaatacag gcttaccaag
     7741 ttccattact atcacacgag tagtgaagat acagtctctc ccccgacagt gctttgatgg
     7801 cgtatcctca cgttaaagaa gaataaccga ctgcctacct tttggtcaga gcctatcaat
     7861 agctttggct ctctctcgat aacgaggttt atcaactgtt cacttacgtt tatctttcta
     7921 cccagtctag ccccttgaag gtgtgctatt cactttctcc caatgtcctc tcacgatttc
     7981 attgactgat tttcatcagg ggttcattgt caggaagctc cgcaccacac cgttaccggt
     8041 gtcgcactat ccctagacta ctgttagcca tataacaggt taccttatca ttccctccgt
     8101 actactcata tcttcggatt gtttgaccgt aacaatgaca gtaatagctt acgcatactt
     8161 actaagtcag tatcctatac tgcataaata agtccgaacc tttacaaaat aaattcactt
     8221 atgcgactaa agtcgctttg tctatatcca aattttcccg caaatagcct ataacctgtc
     8281 caataggcga aggtagctaa ccctagctaa tgtgagtatt agaggtgatt ttaacgagaa
     8341 tttaaggtat aattatccct agctcataaa aacgaataaa caagagttta tccttagtgt
     8401 ttttattcac tattgaggag cattctacga ttaccgtaga ttgcgagtgt gaaaagtata
     8461 agtaaagatg gcactccttc cctaagattt accaagtctt agttctgatg tcttttctga
     8521 gtgctgaggc gatctattgg actttattta tagtaggtgt tgtaaatttt ctctgctctg
     8581 gggtatgggt aactccggtc tgcggatctt gctttcttac tctatcttgg tattaagaaa
     8641 aaaaccaagt acatcaaagt gaagatcata ccttacagcc tttcgagttg gttcatttac
     8701 tttcccaact tagaacaaga gtgcttgggc aaagcccctt ttctcttgcc tgtctttcca
     8761 agcactccga agagtgcttg gaaagtcgga agcaggtgat aagaatacca cccacttcca
     8821 gcaataactc cttgatatat aaggagttat tgccataagt acgacaactt gtcgcactta
     8881 acgttttgag tattgtggac gacgacgtgc tttgtgtaaa cccacttttt tacgttcaac
     8941 gcgacgtgcg tcacgagtaa cgaagcctgc tgcacgaagt gctgggcgta gcgtttcatc
     9001 gtattccatt aatgcacgag tgataccgtg acggattgca cccgcttgac cagaaatacc
     9061 accaccttta acagtgatgt aaaggtctaa tttatcagtt aattcaacca attctaacgg
     9121 ctgacgtacg atcatacgag ctgtttcacg accgaagtaa acttcaagtg aacgttggtt
     9181 gatagtaatg ttacctgaac ccggtttgat aaatacacgg gctgaagagc ttttgcggcg
     9241 acctgtgccg tagttttgat ttgctgctgt catttttatg ctccgtgaat tagatatcta
     9301 aaacttgtgg ttgttgtgct tggtgattgt gttctgaacc tgcgtaaact tttaatttac
     9361 ggaacatttc acgaccaagt ggaccttttg gtaacatacc tttcactgcg atttcaatca
     9421 cagcttcagg acgacgagca atcatttctt tgaaggttgc atctttgatg ccacctacat
     9481 agccagtgtg ccagtagtag attttgtcag tttgtttttt acctgttact gctactttgt
     9541 ctgcattgat cacgatgatg taatcaccgg tatcaacgtg tggagtgtac tcaggtttat
     9601 gtttaccacg aagacggcgt gccaattcag tcgctaaacg acctaatgtt ttacccgtcg
     9661 catctactac ataccagtca cgttgtactg tttctggttt tgctacaaaa gttttcatta
     9721 attaaatacc aaatttaaag tttaaaaccc aatacctcat tgaggtattt cctattattt
     9781 tacggtgaac ccgacccctt cgagtctgat tcagaccaaa cttgtgtggc ggggaaaacc
     9841 acacaaaaac agggtggcga aattatacag agatttcact caaaaatcta acattttttg
     9901 ctgataatct ttcaaaattg aacctatatc acattttaat catttggagc ttttcaaata
     9961 aaaactgaaa tgttaaaaat agcagtaatc aatcactaaa acataaggag tttatgatgg
    10021 aacaatggac aacagatgtt tgggcagcca ttatggcagc atttattgta ggtgctgtaa
    10081 taggttgcac tctagttaga gtatttaaca gtagtgtcag aaaacagcat gaactggaaa
    10141 aagagttgaa gcaaaccaaa actcaagtgg atgagcaaaa acaaaaactt gaacaacact
    10201 ttgagcaatc agcccaatta ctttcaagtc tagcttctga ttataaaaag ctttataccc
    10261 atttagcaca aagctctgaa aagctattac cagaagcatc aaaaaatatt acctttttcc
    10321 aacaaaccaa attacctatt ccggcagaaa aaacggaaga agatcaacct agagattatt
    10381 ccgaaggttc atctggctta ctgaaatcat aaaactaaaa attgaatatt tagttttact
    10441 aaaaggcact tagtttaagg attgattccg ttggaatcaa tcctttattc taaaccgcta
    10501 cattaagcag ttaacaacat ttttcagctt tagatactga acttaggtta taagaagcct
    10561 attttgtaag agttattccc actcaatggt tgccggtggt tttccgctca cgtcatacac
    10621 cacacgggaa ataccgttca cttcgttaat aatgcggttg gagattttgc ctaatagatc
    10681 atacggcaag tgcgcccagt gagcggtcat aaagtcgatg gtttccaccg cacgcaggct
    10741 gacgacccag tcatatttgc gtccatcgcc cattacgccc accgatttca ccggcaagaa
    10801 taccgtgaaa gcttggctga ctttgtagta ccagccggct ttgtgcagtt cttcgatgaa
    10861 gatcgcatcg gctttacgca gtaaatcgca gtactctttt ttgatttcgc ccagcacacg
    10921 cacgcctaag cccgggccgg ggaacgggtg gcggttgagc atttcagccg gtaagccgag
    10981 tgccaagccg attttgcgca cttcgtcttt aaacaattca cgcaacggtt cgactaagcc
    11041 gagcttcata taatccggca agccgccaac gttatggtgt gatttaatca cgtgggcttt
    11101 gccggttttg ctggcggcgg attcaatcac atcagggtag atcgtgcctt gtgcaagcca
    11161 tttgaccgag ctaagttttt tgctttcgtc atcaaacacg tccacaaaca ctttaccaat
    11221 ggttttgcgt ttagcttccg gttcgtctaa gccttttaac gcttctaaga aacggtcttc
    11281 ggcattcact cgcacaatgt tcaaaccgaa tttgttgccg aacatttcca tgacttgatc
    11341 gccttcgttc aagcggagca agccgttatc cacaaatacg cagtgcaggt ttttaccgat
    11401 agcacgatgt aatagcaagg cggtgacgga ggaatccacc ccgcccgaca aacctaaaat
    11461 cacctcgtca tcgcccactt gctcacgaat gcgtttcacc gcatcttcaa tgatgttttc
    11521 tgccgtccag ttcgtttcac agccgcaaat gccgaccacg aagtttttca gcaacgccaa
    11581 gccttgtttg gtgtgggtca cttcggggtg gaactgcacg ccatagaaac ggcggctttc
    11641 gtccgacatc gcggcaatcg ggcaggttgg ggtaacgcct gtgatggtaa agtgcggtgg
    11701 taatttggtg actttgtcgc cgtgactcat ccacacgtcc aattttggct cgcaagcggt
    11761 caaatcatcg tttaaatttg caaaaagttg atcggcagat ttgagagcca ccgaggcata
    11821 gccaaattca cggtggtcgg acgtttcggt taaaccgccc aactgcatcg ccattgtctg
    11881 cattccgtag caaatgccca gcactggcag accagcgtta aacacatact ccggcgctcg
    11941 tgggctgttt tcctcagtgg tgctttcagg acctcctgaa agaataatcc ccgttgggtt
    12001 aaattcacgg atctgctctt ccgttacgtc ccaagcccaa agctcgcagt aaaccccaat
    12061 ctcccgcaca cggcgtgcga taagctgagt atattgtgaa ccgaagtcta aaattaaaat
    12121 tttgtggtgg tggatgtttg tcattgtaat ttcctttaaa ttatggcgtt gtggtcatgt
    12181 taataaaagt gccgcctacc ccatcgtttc tatatgtaat aataaaagat tgcgttttat
    12241 tacaacctgt tgcagtccaa agttcttttg cattaccttc acttggaata ttgatgattt
    12301 ttgtatgaat tttatcagga gtacatttat gtattgcttg aaaatacaga tttatatttc
    12361 tttctgtgtc caactttaat aaaggactgg ccgttgtttc tcctgaataa gatggtccga
    12421 aaatactgca cgcagataaa gcgacgattg aa
//
LOCUS       DANLXQ010000072        12343 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.072, whole genome shotgun sequence.
ACCESSION   DANLXQ010000072 DANLXQ010000000
VERSION     DANLXQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.072"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            128..1318
                     /locus_tag="PXD94_001497"
     CDS             128..1318
                     /locus_tag="PXD94_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL6286354.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPIVISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGESGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFNLDWALEKIR
                     EVLA"
     gene            1393..2904
                     /gene="nhaC"
                     /locus_tag="PXD94_001498"
     CDS             1393..2904
                     /gene="nhaC"
                     /locus_tag="PXD94_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL6286355.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     VAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGCCP"
     gene            3021..6620
                     /gene="putA"
                     /locus_tag="PXD94_001499"
     CDS             3021..6620
                     /gene="putA"
                     /locus_tag="PXD94_001499"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL6286356.1"
                     /translation="MMSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNI
                     KKLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLI
                     RDKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPL
                     IRKGVDVAMRMLGKKFVTGETIQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYY
                     NDYVASIHAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLL
                     AKKYDIGLNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHL
                     IETARKHNRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLA
                     AQDVIYPQFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIY
                     APVGTYQTLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQAPVQKAELTNGTMHPK
                     IPLPVDLFDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESR
                     KIYNPANQAELVGEVIDATENDMEQAFESAAAFQSEWANTSPAVRAECLEKMADLMEA
                     NMPQLFDLAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPW
                     NFPLAIFVGEVSSALVAGNVVLAKPAEQTSLMAHYAVSLFYQAGVPKAALQCLPGRGK
                     TVGVALVADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALG
                     EQVVIDVLNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDV
                     GPVIDKVAQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHE
                     VFGPVLHVIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNR
                     NTVGAVVGVQPFGGMGLSGTGPKAGGPLYLQRLVNRQEWTLNGLQGNAQAVDFDLNFA
                     IRKVLSGAEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGISKGNLA
                     SQVQALIAIVTVGSQAVAEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSS
                     EEKLHHAERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
     gene            6729..7379
                     /locus_tag="PXD94_001500"
     CDS             6729..7379
                     /locus_tag="PXD94_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha3 family transposase"
                     /protein_id="HDL6286357.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVIARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEIDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFWSQAKRHLRKFNGIPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(7380..8267)
                     /locus_tag="PXD94_001501"
     CDS             complement(7380..8267)
                     /locus_tag="PXD94_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide transport protein"
                     /protein_id="HDL6286358.1"
                     /translation="MNKLTQLALISLAFTGSAFAAPKSFTYCLESSPAALNPQIATDG
                     ATLDVGQAIYNRLIDFEKGTTNLTPSLAERWEISEGGKIYTFFLRQNVQFQSNKSFTP
                     SRYFNADDVIFSINRQFDKTNPYHKLSGGNYEFFYGMNMQNIIKNVTKVDDYTVKIEL
                     SEPNAPFLANLAMDFMAIFSAEYANKMQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVR
                     FTAFEHYWEGKAKIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLSALKANKDIQ
                     LFEKSGLNIGYITFRGCPR"
     misc_feature    complement(8293..8438)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(8615..9625)
                     /gene="mglC"
                     /locus_tag="PXD94_001502"
     CDS             complement(8615..9625)
                     /gene="mglC"
                     /locus_tag="PXD94_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL6286359.1"
                     /translation="MSALQQNRTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSN
                     ILSQSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSE
                     IPIPVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSP
                     IAGFSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPE
                     AARVSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGG
                     VSFAGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAK
                     KK"
     gene            complement(9639..11138)
                     /gene="mglA"
                     /locus_tag="PXD94_001503"
     CDS             complement(9639..11138)
                     /gene="mglA"
                     /locus_tag="PXD94_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL6286360.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVETIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDSMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEEILQLAAKYL"
     gene            complement(11210..12199)
                     /gene="mglB"
                     /locus_tag="PXD94_001504"
     CDS             complement(11210..12199)
                     /gene="mglB"
                     /locus_tag="PXD94_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL6286361.1"
                     /translation="MKKAVLSAVAMAVALGATISTAQAENRIGVTIYKYDDNFMALMR
                     KEIEKEGKTQKVELLMNDSQNTQSIQNDQVDVLLSKGVKALAINLVDPSAYKTIIQKA
                     KSEDVPVVFFNKDPGAAAIDSYPKAYYVGTEPKESGIIQGELIAKQWKAHPELDLNKD
                     GKIQFALLKGEPGHPDAEARTKYVVESLKAQGIDSEQIFMDAALWDAAQAKDKVDAWL
                     SSGKAKDIEVIISNNDGMALGALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVL
                     NDGVNQGKAVVQLSANLADGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
BASE COUNT         3554 a         2599 c         2641 g         3549 t
ORIGIN      
        1 gcatagttaa tcaacaactg aagaaaaatt agaagtagat cataaaatca acaaaaagtc
       61 cgtgcttttt agctaaagat tgttaaactc ttttgtgtta caagttttta acataaagga
      121 gttttttatg tctacttctc aacaactcat ccaacaaacc gaacatttcg gtgccaataa
      181 ctacctacct ctacctattg ttattagtaa agctaaaggc atttgggttg aagatcctga
      241 gggtaatcgc tatttggata tgcttagtgc ctactcagcg gttaatcaag ggcattgtca
      301 tccaaaaatt gtacaggcac taaaagatca agcagacaaa gtaacgctga cctctcgagc
      361 attttataac gatcaacttg gtatttggta tgaaaaaatc tgcaaactcg ctaataaaga
      421 gatggcatta ccgatgaata cgggtgcaga agcggtagaa acagcgatta aaactgctcg
      481 ccgttggggt tatgatgtga aaggaattgc agaaaatcaa gcagaaatca tcgcttgtgt
      541 tggcaatttt cacggacgaa caatggctgc agtatctctc tcgtctgatc cggattataa
      601 acgtggtttc gggccgatgt taggtggcat caaacttgtt ccatacggtg atttagaagc
      661 attaaaagct gcgattacac caaacacagc agcgtttttg gttgagccaa tccaaggtga
      721 atctggcatt cttattccac ctgaaggttt cttggaacag gcttatgaac tatgcaaaga
      781 gaataatgtg ctgtttattg ctgatgaaat tcaggcaggt cttgggcgta ctggcaagtt
      841 atttgcaaca gaatgggaag gctttgagcc agatatttat attctaggta aagcacttgg
      901 tggcggggtg ttcccaatct cttgtgtgtt agcaaatcgt gatattttag gcgtattcaa
      961 tcctggctcg cacggttcga catttggtgg taatccatta gcctgtgcgg tatctattgc
     1021 ctcaattgat gtactgttgg aagaaaaact tgcagagcgt tcccgtgagt taggtgaata
     1081 cttcttggca gagctgaaaa aaatccaaaa tccaattatt aaagaagttc gtggacgtgg
     1141 actgtttatt ggcttggagt tacatcaacc agcacgccca ttctgcgaaa aactcaaaga
     1201 aaaaggctta ctctgcaaag agacccacga tacagttatc cgctttgctc cccctttggt
     1261 gattgaaaaa ttcaatttgg attgggcatt ggagaaaatt cgggaggtgt tggcttaatt
     1321 gtcattttct ctatatttta tcctgccaaa tcgcctttaa ccctttttgg caggaagcaa
     1381 aacagttgta aaatgctgat tttgcaaact ttttccaaaa atcaaccgct tgtaaaaggt
     1441 ggctttatgt caaacagaac agaaataaca gaccaaaata tccgaatccc gtttcgctat
     1501 gctatattgc ctgtgattat tatggtatcg attatggcgg tggctatcat caaatttgaa
     1561 gcctcacctc acgttccgtt gcttattggg gcaattagtg ccgctgtgat tgcaatggca
     1621 catggctacc gttgggagac aattgaaaaa ggaatttata gaggcattcg tatggcttta
     1681 ccagcgattg ttatcattat tatgattggg ctaaccatcg gagcttggct aggtggtgga
     1741 attgtagcta cgatgattta ctacggcttg aaattgatct ctccgtcgct gtttttagta
     1801 actatctcaa taatttgtgc tattgtgacc cttgcgattg gtagctcgtg gtctacaatg
     1861 ggaacaatcg gtgtagcggc gatggggatc gggtttagtc tcggcattcc ggcaccgatg
     1921 gttgccggtg cggtgatctc aggagcatat ttcggcgata aaatgtcgcc actgtcagac
     1981 actaccaatc tggcagcagg gattaccgaa accaatctgt ttgaacatat ccgccatatg
     2041 tttatcacta ccgtgccggc gttcctgatt gcgttatctg tctatttcta ttttggtatg
     2101 gaatttagag ccgaaaatct cgatcttgct aaaattgaag acattatgca ggcaatgcag
     2161 gaacatttct tgatttcgcc ttggttgcta ttagtgcctt tattagtcgt ggtgttggtg
     2221 gcaaaaaaag tgcctgcctt accggcatta gctgcaggga taattcttgg ttggctctgc
     2281 cacgtttttg tgcaaggcgg tgatgtcagc gtagcagtga ttactctgca cgacggctat
     2341 aaaattgaga gcggccacga gatgattgat aagctgttca accgtggtgg gatcgagtcg
     2401 atgatgtaca ccatttccct tacgattgtt gcgatgactt ttggtggcat tgcagagcaa
     2461 gctggaatgt tacattcagt agtggaaaaa atccttaccc ttgcaaaatc agcaggtagc
     2521 ttaattagtg ctacgattgt atcggcattt attaccaacg taacgacatc tgagcaatat
     2581 atttctattt tattaccagg cagaatgtat ataaaagcct atagggataa gggactaagt
     2641 tcgagaaacc tttctcgtgc attggaagac ggaggaacag ttacttcggt tcttgtacct
     2701 tggaatacct gtggtgttta tgcgttctca atgttgcaag tgagtgcttt tgaatatgca
     2761 ccgtatgcaa taatgaattg gctcattcca ctgattgcga ttggcttggc aaccttgaat
     2821 atcaaaattg agcgattgcc accggaagaa ttagccaaag aacctgagga aattactctg
     2881 ccaaaacgtg gttgttgtcc ataatttatc taaatagtat ttaataacaa taggttaagt
     2941 taaataacaa gcggtcggtt ttatcttaaa atttgcaagt ttatacataa atttgatcgc
     3001 ttgctgtata gaaaggaaaa atgatgtcat accaactttt acctaacacc actatccgtc
     3061 ctgaattgag ccgtcattac cgctttgatg agcaaaccct ggtgcaaaat ctgatagaac
     3121 aagcacattg tgatgaacag gctgataaca ttaaaaagct cactacccaa ttagtcggta
     3181 aagtccgaac tgcacgccag aaagcaagcg gggttgatgc gttaatgcac gaattttcac
     3241 tctccagcca agagggggtg gcgttgatgt gccttgccga agcattacta cgcattcctg
     3301 acaccgccac tgcggataaa ttaattcgag acaaaattgc caaaggggat tggcgttcac
     3361 acaccggcaa cagcccctct ctgtttgtga atgcagcggc gtggggcttg gtgattaccg
     3421 gcaaattggt ttcaacccac agcgaaagct cactctcatc agcactttca cgcctgatcg
     3481 ccaaaggcgg agaaccgctg atccgcaaag gtgtggacgt agcaatgcgt atgctcggca
     3541 agaaatttgt aacgggcgaa accattcaac aggcgattaa aaacggcgaa aaacgcttcg
     3601 caatgggtta tcgttacagc tacgatatgc tgggcgaagc ggcactcacc gccgaagatg
     3661 ccaaacgcta ttacaacgac tatgttgcct ctattcacgc tgtgggtgcc acctcgaaag
     3721 gcttgggcgt gtataaatcc tcgggtgtgt cggtcaaact ctccgccatt caccctcgtt
     3781 attccctttc ccaacaccaa cgtgtgatgg acgaactcta tccacgtctg aaagaactgt
     3841 tcttgctggc gaaaaagtat gacatcggct tgaacattga tgccgaagaa gccgaccgcc
     3901 tcgaattgtc gcttgatttg atggacagac tgcttgccga ccctgatttg gcagggttta
     3961 acggcattgg ttttgtggtg caggcgtacc aaaaacgttg cccgtatgtg atcgagcatt
     4021 taattgagac cgcccgtaaa cataatcgcc agctaatgat tcgcttggta aaaggcgcat
     4081 attgggacac tgaaatcaag cgggcgcaaa tggacggtgc agagggctat ccggtcttct
     4141 cccgtaaggt gcataccgat ttgaactaca tcgtttgtgc caaaaaattg ctggcggcgc
     4201 aagatgtgat ctatccgcaa tttgccaccc acaacgctca aactctcgcc accatttacc
     4261 acttggcaca gggcaaacgt tttgagtttc aatgcttgca cggtatggga gagagcttgt
     4321 acgaccaagt ggttggcagc gataatctta acgtgcaatg tcgcatttat gcccctgtcg
     4381 gtacttatca aaccctgctt gcctatttag tacgtcgctt gttggaaaac ggagcgaaca
     4441 gctcatttgt tagccaaatt gtggatgaaa atctcagtgt agatgatctg gctcaagccc
     4501 ctgttcaaaa agcagaactg accaatggca ctatgcaccc gaaaattcca ttgccagtcg
     4561 atttatttga cgacaatcgt gttaattcca aaggctatga tttaactgat gcagtaagcc
     4621 tacaaaattt agaaaatgcc ctcaatgaac ttaccaaaca gcattaccgt gctgaaccac
     4681 agctggcaaa tgataataca agcggtcaag aaagccgaaa aatttacaat ccagcgaacc
     4741 aagctgaatt agtcggcgaa gtgattgatg ccaccgaaaa cgatatggaa caggcgtttg
     4801 aaagtgcggc ggcgttccaa agtgaatggg cgaatacttc ccctgcggtg cgtgccgagt
     4861 gcttagaaaa aatggcggac ttgatggaag ccaatatgcc acaactgttc gatttggcgg
     4921 tgcgtgaggc aggcaaaacg ctcaacaatg caattgctga agtgcgtgaa gccgtcgatt
     4981 tctgtcgtta ttatgccaaa gaggtacgca acaacattga agcatacaaa aatccgcgtg
     5041 gcattgtagt agcaattagc ccgtggaact ttccgttagc gatttttgtg ggtgaagttt
     5101 catctgcgtt ggtggcaggc aatgtggtac tagcaaaacc agctgaacaa acttcgttaa
     5161 tggcacatta tgctgtcagc cttttctacc aagcaggtgt gccgaaagcg gcgttgcaat
     5221 gcttaccggg tagaggaaaa acggttgggg tggcgttggt ggcagatcat cgtgtgaacg
     5281 gcgtgatttt tactggttca acagagacag ctaaaaacat taaccgcaat ttgcacaaaa
     5341 acgagaaaat tataccgctt attgccgaaa ctggcggaca aaatgcaatg attgttgata
     5401 gctcggcact gggcgagcag gttgtgatag atgttttaaa ctccgcattt gactcagcag
     5461 ggcaacgttg ttcggcattg cgtgtgctgt atctgcaacg tgatgttgcc gatcacattc
     5521 tcacaatgct caaaggagca atggcggagt tgaaagttgg tcttccacaa cacttaacta
     5581 ccgatgtcgg accagtaatc gataaagtcg cacaacaacg cttgctcgat catattgaaa
     5641 atctcaaaaa atcggcgaaa agttactatc aggtggctat ctcctctgaa attgccgaaa
     5701 atggtatttt cgtaccgcca acgctgttag aaatagatca tatcagccag cttaaacacg
     5761 aagtattcgg accggtgcta cacgttatcc gttttgatgc ggataaactc gaccaagtga
     5821 ttgccgacat aaactccaca ggtttcggtc taaccagcgg tttgcatagc cgaattgatg
     5881 aaacggtgga tttatggttg aaatctgtcc acgctggaaa tctgtatgta aaccgtaaca
     5941 cggtaggggc tgtggtcggc gtacagccat tcggtggaat ggggttatca ggaactgggc
     6001 cgaaagctgg cgggccgctc tatctacaac gtttagtaaa ccgccaagaa tggacattga
     6061 acggtttaca gggcaatgca caagcggtcg attttgactt aaattttgca atccgcaagg
     6121 ttctaagtgg agctgagcag caaacggcgt tagatctggc caaaagcctt gcacgccgct
     6181 cgccactcgg aaaaacctac ccaatgcagg gtattaccgg cgagaaaaac gagatgattt
     6241 tcgtggctcg cccacagatt gggattagca aaggtaattt ggcttcacag gtgcaagcct
     6301 tgattgccat tgtaacggtc ggcagccaag cggtggcaga aaaatcaagc gagctggcaa
     6361 aacacgcctc tgcctttggt aagaaattac aggtggtgga tgatttcaac agtctcgatc
     6421 acctcagcac actcattgtg cttgacctgc tttctagtga ggaaaaattg catcatgccg
     6481 aacgcaaagg ggcgattttg acctacatcg aagggctgga tttagcgctc tcactgctgc
     6541 cgttggtaca cgagaaagca atcagcatca acaccgctgc cgcaggggga aatgccagct
     6601 taatgagtga aatggattaa gcaacaaaca aaagagtata agagggcaga tcacaatgat
     6661 ctgccttttt attttaaggg gctgtcctag ataacgacta aaacttccat ttaggttaag
     6721 ataaccctat gagaaaaagt cgtctaagtc agtacaaaca acataaactc atagagctgt
     6781 ttgtagcagg cgtaatcgct cgaacagccg cagagttagt gaatgtaaat aaaaccaccg
     6841 cagcctatta ttttcaccgt ttgcgattgc tcatctatca acatagccct catttggaaa
     6901 tgtttgaggg agaaattgag attgatgaga gctatttcgg cggtcatcgc aagggtaaac
     6961 gaggtcgtgg tgcagcaggc aaaaccgccg tattcgggct tttaaagcgt gacggcaagg
     7021 tttataccgt agtagtgccg aacacacaat ctgctacact tttgccgatt attcgggaaa
     7081 aggtgaagcc cgatagcctt gtttacaccg atttttatcg aagctatgat gtgcttgatg
     7141 tgagtgaatt taaccatttt cgtatcaatc acagcacaca cttcgcagaa aatcaaaatc
     7201 acattaacgg aattgagaat ttttggagtc aagcaaaacg tcatttacgc aagtttaatg
     7261 gtattccgaa agcccatttt gagctatatt tgaaagaatg cgaatggcgt tttaatcaca
     7321 gtgacttaaa atctcaaata ttccttttaa aacaattggt taaagggggg ttaggttagt
     7381 tatctaggac agcccctaaa agtaatataa ccgatattta ggcctgactt ttcaaataat
     7441 tgaatatctt tattcgcttt taaagcagac aaatctgacg ggttcggata aggcatcgct
     7501 tggcattcgt tcttctccaa tttagctaaa cgtactgagg catcgggcgt aatactaaat
     7561 acaaggcgat ctatcttcgc ttttccttcc caatagtgtt caaatgctgt aaatcttacc
     7621 gctgcgtcct tttggtaacc atagaactgg aatgcacctg tgcctatagg ttctaaatct
     7681 aatttttccg gcgtacccgc tatttgcatt ttatttgcat attccgcaga aaagatcgcc
     7741 ataaaatcca tagctaaatt agcaagaaag ggggcattcg gttcagataa ttcgattttt
     7801 accgtgtaat catctacttt tgttacattt ttgatgatgt tttgcatatt cattccgtag
     7861 aaaaactcat agttaccgcc ggacaatttg tgatatggat tcgtcttatc aaactgacgg
     7921 ttaatcgaaa aaataacatc atcagcatta aaataacgac taggggtaaa acttttatta
     7981 ctctggaact gcacattctg acgcaagaaa aaagtataaa tttttccgcc ttcactgatt
     8041 tcccatcgct ctgccagaga aggagtgagg ttagtggtac ctttttcaaa atcaatcagg
     8101 cgattataga ttgcttgccc gacatcaaga gtagctccat ctgttgcaat ctgaggattc
     8161 aatgctgccg gagatgattc caaacagtaa gtaaaagatt taggtgctgc gaaagcagaa
     8221 cctgtaaatg ctaaagaaat aagtgcaagt tgtgtcagct tgttcattgt catatcctta
     8281 aatgttaagc aataaaaaac cctttcaaaa gttgccttcc gaaagggtta gaattgggtt
     8341 tgcgattttg gattgcgttt gccatttcag gttttcggaa gatatcttcc gaacacgcca
     8401 aatgcccgaa agattaatcg gtcaaatggt agtgatgatg tttaacgcaa tagctcataa
     8461 tattctccaa aattgaatgt ttaggaattt acgcttttta aaaatcaatt gcaaacgttt
     8521 tctttaaaat tttgtaaaaa attcaacatt tttcaccgct tgtatcgtac ctaggagcaa
     8581 atggcgattt gctcctaggt ttccaagcaa taggctattt tttcttcgca tatttaagtg
     8641 agtcaatcgc taccgccaag ataatgatgc cacctttaat aatatattgc cagtaagggt
     8701 ttactccaat ataggttaag ccgtagttga taacggtgaa gattaacacc cctgttacta
     8761 caccgatgat agtacccaca ccaccggcaa aggatacccc acctaccaca caagcggcaa
     8821 tggcatccat ttcatacata aagcctaagt tgttggttgc actaccgata cgacctgctt
     8881 ctaacatacc gccgaaggca tagaagatgc ctgaaagcat ataaataccc atcaggttaa
     8941 gcggtacgtt tacaccggaa actcgtgctg cttcagggtt gccgccaata gcaaatacgt
     9001 ttttaccgaa gcgggtttta ttccataacg tccacataat aatggtagca atcacggcat
     9061 aaatagtgat gtaggatagc ctaaagtcgc caaagcggaa gaagccttgt gcaaaggttg
     9121 agaaatcatc gctaaagcct gcaattggcg atgagcctac tgcatcgtag taaagcgagt
     9181 tgataccata cacaataatc attgttccca tcgtggcaat aaacggtgtt acgttgaggt
     9241 aagccaccac aaagccgtta aataaaccaa tgacagcacc gatggcacac acggttaata
     9301 caaccacagg aatcgggatt tccgagaggt ttgggaatac cttgttcata ttttccattg
     9361 attggagcaa ggtggcggaa attaccgctg ccaagcccac ttggcgaccg gcagataagt
     9421 ctgtcccttg tgttacaatc aagcctgcaa cccctaatgc aataatcaaa cgcacagaag
     9481 attgcgataa aatgttactg aagttacgca agctcaagaa acttgggtct tgcacaataa
     9541 tcacaataag taagattaat aatacgaagt aaatcgcatt ttgtttaaaa aaatcgagtg
     9601 ttctattttg ttgtaaagca gacatacttc actccttgtt ataaatattt tgctgcgagt
     9661 tgcaagattt cttcttgcga ggtgtgagcg gtttcgacaa tacctgcaac tttcccgtta
     9721 ctcatcacta aaattcggtc tgtgacccca agcaattccg gcatttccga tgaaatcata
     9781 atgatgcctt tatctttatt cgccagctct aaaatcaact ggtaaatttc aaattttgcc
     9841 ccgatatcga taccacgggt cggctcgtca agcatcaaga tttcaggctg ggttaacaac
     9901 caacgcccga taatcacttt ttgttggtta ccgccggaaa gtgagccaat atgcgtttga
     9961 tgcgatgggg tttttacatt catcgaatca attacccatt gggtatcgct tttcattttt
    10021 ttattgctca gtaagccgaa cttgcctaga taagacttca tatttgaaat cagagagtta
    10081 aactcaatgc ttaagttggc ataaatccct gttgaacggc gttcttctgt aaccaatgca
    10141 aagccgttat taatcgcatc aaaagcgttg cggttattga tttctttatc gtttagtcgg
    10201 atagtgccgc ctgctttctc ccgtacaccg aaaatggttt caacaatatc agtccgtttt
    10261 gctccaacta agccggcgat gcctaaaatt tcccctctgc gtaagctaaa gctgacatct
    10321 tgaatagagg gttgattttt tgccgttaaa tgctcaactt caagaatcac ttctttcggc
    10381 agattggttt tttcagggaa acgctgggtc agctcacgcc ctaccatcat tgaaaccact
    10441 tgatccattg aggtatcttt aattgcaacg gtgtttaccc atttaccgtc acgcaaaatg
    10501 gtaatttcat cacaaatttt aaaaatttcg tccattttgt gtgaaatata gataatgcca
    10561 cagcctctgt ctttcagttt ttggataatt ttaaataaat gctcgacttc tttttctgaa
    10621 agtgacgaag ttggttcgtc cataatcaca attttggcat tgtaagagaa agcttttgca
    10681 atctcgatca tctgcatttg tgaaaccgat aatttggcta ctttatcacg tggatcaaca
    10741 tcaatatcta attcatcaaa aatcgcctta gtatcacgat acattttatc gtgatctaca
    10801 aaaggccctt ttagtgggta acgacctagc cataagttat ccattaccgt cacttgtttt
    10861 actaggttca actcttggtg aaccattgag atcccatttt ctaacgcctc ttttgaggtc
    10921 ttaaaattca ccggcttgcc aagaaagaga atatctccct catctttagc ataaatccca
    10981 aataagcatt tcagtaacgt tgatttacct gctccattct ctcccattaa ggcgtgaaca
    11041 ctatgggatt tcaccgttaa gttagcatta tctaaggctt ttaccccggg aaatgacttg
    11101 ctaacatttg tcattgtgag taaaacttca cctgtcatat ctcacccctg attacatcga
    11161 aaaatcgaaa aaaagcacgg gtatccatca cccgtgctaa aagcgtgaat tattttaaaa
    11221 attcagataa gttgtcttta tcgacaccaa cgtaaggaat acgcaccaca cggttttcca
    11281 atttccattg tgtaccagca actgcatctt taccgtctgc taagttagca gaaagctgaa
    11341 caaccgcttt accttggttt acaccatcat ttaataccgt accggctaat tcaccttttt
    11401 tgattaattg aagtgcttct ggcaatgcat ctacaccgaa gattggtaat tttctaccgt
    11461 gagctttggt tgcttctaat gcacctaacg ccataccatc attattagaa ataattactt
    11521 cgatgtcttt cgctttacca cttgataacc aagcatccac tttatcttta gcttgggctg
    11581 catcccataa tgctgcgtcc ataaagattt gctcactgtc gataccttgt gcttttaagc
    11641 tttctaccac atatttagta cgtgcttccg catcagggtg acccggctca ccttttaata
    11701 aggcaaattg gattttaccg tctttattca agtctaattc aggatgggct ttccactgtt
    11761 ttgcaattaa ctcaccttga ataatgcctg actctttcgg ctcagtacct acataataag
    11821 ctttcggata gctatcaatc gcagctgcac ccgggtcttt attgaagaac accacaggca
    11881 catcttcact ttttgctttt tgaataattg ttttataagc tgatggatcc actaagttaa
    11941 ttgctaatgc tttaacccct ttagaaagta atacatcaac ttggtcgttt tgaatagatt
    12001 gcgtattttg cgagtcattc attaataact cgactttttg tgttttaccc tctttttcga
    12061 tttctttacg cattaatgcc ataaagttat cgtcatattt ataaattgtt acaccaatac
    12121 ggttttcagc ttgagctgta ctgatcgttg cacctaatgc gactgccatt gccactgcac
    12181 ttaatactgc tttcttcata gtagatcacc tcttttatgt gtttataggg aaatgaattg
    12241 cttaccataa gcaacttggt gcaaagtata actaatttta aaaaaacaat ccgtgatcat
    12301 tctcacagaa ttaagacaaa gtggagaaat ttttaatttt aag
//
LOCUS       DANLXQ010000073        12333 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.073, whole genome shotgun sequence.
ACCESSION   DANLXQ010000073 DANLXQ010000000
VERSION     DANLXQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12333
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.073"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..218)
                     /locus_tag="PXD94_001505"
     CDS             complement(<1..218)
                     /locus_tag="PXD94_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286362.1"
                     /translation="MSSIKEIFSTKNSPINVLMQTEWFLGFILIVVYLDAYFQLVHQV
                     PIYIAIQQKEIYLTKTIDYIAAIFFFSVT"
     gene            complement(288..1421)
                     /locus_tag="PXD94_001506"
     CDS             complement(288..1421)
                     /locus_tag="PXD94_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL6286363.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
     gene            complement(1552..2580)
                     /locus_tag="PXD94_001507"
     CDS             complement(1552..2580)
                     /locus_tag="PXD94_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6286364.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(2592..4136)
                     /locus_tag="PXD94_001508"
     CDS             complement(2592..4136)
                     /locus_tag="PXD94_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6286365.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(4187..5125)
                     /locus_tag="PXD94_001509"
     CDS             complement(4187..5125)
                     /locus_tag="PXD94_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL6286366.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
     gene            complement(5457..6968)
                     /locus_tag="PXD94_001510"
     CDS             complement(5457..6968)
                     /locus_tag="PXD94_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL6286367.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAAADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
     gene            7187..8011
                     /gene="iolB"
                     /locus_tag="PXD94_001511"
     CDS             7187..8011
                     /gene="iolB"
                     /locus_tag="PXD94_001511"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL6286368.1"
                     /translation="MSSPLLSKPNFNQHEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYAFENIGNRTTPFEKIPPYAVYIPPHKSVLVKTEL
                     NLELAVCKAPSQGNYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEAADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
     gene            complement(8087..9538)
                     /locus_tag="PXD94_001512"
     CDS             complement(8087..9538)
                     /locus_tag="PXD94_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL6286369.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDVEGVG
                     RELSTNPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTIGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQAMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
     gene            complement(9716..11026)
                     /locus_tag="PXD94_001513"
     CDS             complement(9716..11026)
                     /locus_tag="PXD94_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL6286370.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVAAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLSIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDTIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVWAPMIVS"
     gene            complement(11042..11737)
                     /locus_tag="PXD94_001514"
     CDS             complement(11042..11737)
                     /locus_tag="PXD94_001514"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDL6286371.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKWRVGLLATDATLAVDLYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
     gene            11953..>12333
                     /locus_tag="PXD94_001515"
     CDS             11953..>12333
                     /locus_tag="PXD94_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6286372.1"
                     /translation="MKNIETKWLEDFLTLEECRHFSQAAEKRNLSQSAFSRRILALEE
                     VVGVKLFDRTSIPLQLTEQGKLFHSQTRNLLQQLQNNLDELLGHNCNLPNIKFAAAHS
                     LSLSIMPKLIKKLSQTNENFIYSVE"
BASE COUNT         3579 a         2837 c         2406 g         3511 t
ORIGIN      
        1 gtaactgaaa aaaagaaaat tgctgcaata taatcaatgg tttttgtgag atatatttcc
       61 ttttgctgta tcgcaatata gataggcact tgatgaacaa gttggaagta agcatccaaa
      121 taaacaacta tcaatatgaa gcctaaaaac cattctgttt gcataagaac attgataggg
      181 ctgttttttg tgctaaaaat ttctttaatt gaactcataa tttttcctta aatgtaaaaa
      241 aacgtacctc aaccaatttg agatacgttt ctattttaat gtaattatta aatctcactc
      301 acctccaccc accttttctc ctccgccgat tttgctatcg cttccaatac tctggagatt
      361 ttatagcctt cttcaaaatc gggatacatc gggtcatcag cggcgatgcc gttgatgagg
      421 tcacggattt ccaccgcttt ttggtcgtta aaaccgatgc cgtgaccggc actcacacaa
      481 aaagcagcgt agtcgggatg ttccgggccg actaaaatgg ttttgaatcc ttggcgggat
      541 ttatcgtcat cgtgcaaata gagtttgagt tctgccattc gttcttgggt atagctgagc
      601 gtgcctttag tacctgtaac gacataggtc agccccattt tacgcccgca ggaaatgcgg
      661 gagctttcga gcgtgcccat taccccatta gcaaatcgta tgagactact tgcctgatct
      721 tcgttttcta ctttgatttt ttctgctgga ttgtgcggac tagggcgttc ttcaatcact
      781 gtttgcatat cgccgataac acgctcaatg tctgaaccaa ccaaatagtg tgcgatactg
      841 acaatatgtg cggctaaatc tcccaacgta ccaagcccgg ctttggcttt gtagcagtgc
      901 caatcaatcg gggtattagg gttggcgaga taatcttcgt tatgcgtgcc gtagaaatgc
      961 accacctcac caatttcgcc gttttgaata atttggcgag ccagttgggt ggttggattt
     1021 ttgatatagt tgaaaccgac caaggtttta actcccgctt gttgagcggc aatcaccatt
     1081 tctttggcat cttgggcggt gagtgcaagc ggtttttctg aatagacgtg tttgccgtgt
     1141 ttaatcgctt ctaaggcaat ttctttgtgc agaaaatttg gtgtacaaat gtccaccaca
     1201 tcgacattcg gatcggtaac caattttcgc caatcgtcag tagcacgctg aaagccaaat
     1261 tctcgggctt tttgatcggc taatgcttgg ttgatttcgg caaggtattc cagttggatt
     1321 ttgcctttaa gtggaaacag ggttggggct tgagcgtagg caatggcgtg gcagcgaccg
     1381 atatagccgg tgccaactaa gccgattttg atggttttca taatactatc ccagtagggt
     1441 gcgtttgcta acgcaccatt agaattttca gtgcattggc aaacgcaccc tataagaaag
     1501 cggtctggtt ttgcaaaatt tttacaaaat cagaccgctt gtgttagcta tctataatgc
     1561 tcctcgtctg ctcttcgcat aggtgtcgaa ggcaacggca aggatgatga ttaagcccat
     1621 aatgatttgt tggtagtaag cggaaacgtc cattagtact aaaccgttaa tcaatacacc
     1681 cataataatg gagccgatga tggtgcctgt gactcgtccg tatcccccca ttagggaagt
     1741 tccgccgata accactgagg caattacacg tagctcaaaa gaaacgccgg caacggattc
     1801 ggcactgcct aaacgggcgg agagaatgaa accggcaagt cctgccaatg ctccgatcac
     1861 cacataaaca ctgactaagg tacggttaat attcacaccg gctaaacggg ctgcttccgg
     1921 attgccacct accgagtaca catagcgtcc ccagcgggtt ttatgcaatg cgaagtaacc
     1981 gataatggcg actacggcaa aaatcactac cggcaccggt acgcctaaaa tatcgccacg
     2041 tccccaccag cggtaacctt cgtcaaatcc ggcaattggc gagccgttat ttaacacaag
     2101 tgttaaacca cgccaaattg tcataccgcc aagcgtgata ataaacggtg gcagtttgag
     2161 tttagtgacg cctaagccgt gtaaccaacc aatgaaagtg cctaaaccta aacagatact
     2221 caaacctatc agccagctca ttccgcccca agcaccggct tctagcgtta ccgtattatc
     2281 gcctttgata atataggccg cgaacattgc ggtaacagca aggattgagc cgacagagag
     2341 atcgatccct gcagttaaga tgacgaaagt cattcctacc gccataatgc cgaaaatcga
     2401 cacttcggtt aaaacgttat agatgttgcg ttgcgataaa aatgccccgt tttgtgaagt
     2461 gaagaaaata atcagtaaga tcaagaaaat cagtacgccg aaacgttcaa agaaggcgat
     2521 aaaatcgatt tttcctttgt catcgaccat acccatattt tttagaaatt gcgttgtcat
     2581 acaaacctct cttatgctgc cgctttacgc tgaacgccaa cagccatcat tgacattaat
     2641 ttttcttcgg tggcatcatc gccgtgaatt tcgccagtta cccgaccttc ggaaagagtg
     2701 ataatgcggt cggaaatcgc cataatttcc ggtaaatcgg aggaaatcac taataccgca
     2761 acaccttgtt ttgccatatc aaacagcact tggtgaacct cagattttgc cccaacatca
     2821 atacctcgtg tcggctcatc aacaattagt actttcgggt tgagtgccat acaacgggct
     2881 aaaatcactt tttgctgatt accacccgat aatttgcggg cttcaagttc gctacttgcc
     2941 attttgatgc gtaacgcttg gcggtaggtt tcgattaaat catcttcttt ttgattatcg
     3001 acaaacaagt tacccttcaa taatttgctt aaaatcggca gggtcatatt ggttttaatc
     3061 ggtaaaccga gaactaagcc ctcttttttg cgatcttccg gcacaagggc aacccctttt
     3121 tccaaagcat ctaacggcga ggtggcagca taaggcttac cgtctaggct gatttcatca
     3181 gccgtataat tttctacccc aaacaagcaa cgggcaattt cagttcgccc tgcgccaact
     3241 aagccggcga tccctaatac ttcaccctcg tgaatgttaa aagagacacc gtggagagca
     3301 atgccgtgtg gatcgaatgc cggtttttcc cgatgcagat ttttcacagc taaacgtatt
     3361 ggtcggtggg tatggtgagt ctcttctggt ggacggcgag taaaggcaac ttcacgtcct
     3421 accatcattt taataatttg atcgacattc acatttttga cttcaccctc accagaatag
     3481 cgcccgtctt ggaaaacggt gaaacggtcg catagggcaa acacttcgtt taagcggtgg
     3541 gtcacataaa tcacgctaat gccacggctt tttaaatcac gcacgatttg gtgtagtgtt
     3601 tccacttctt tatcacttaa ggcagcggac ggttcgtcca taataatcaa cttagcgttt
     3661 aaggtcattg cacgggcaat ctcgaccatc tgctgttgtg ccacacttaa acgggaaact
     3721 tgggtgtcag gtttgatatt taagccgaga ttatccagca ctaattgtgc ttcctcattc
     3781 accgcttttt cattgacgaa taaacctttc accggctctc tgcccaaaaa gaaattttca
     3841 gcaaccgtca tattcggtaa caagttaaat tcttgataaa tcgttacgat gcctttgagt
     3901 tggcgatcaa acggggaatc gctcaaactt aaggtttcac cgttaaagat aatttcgcca
     3961 cttgtttgtg gctgagcgcc tgaaagtgct tttaataagg tcgattttcc tgcaccgttt
     4021 tcacccaaca atgcgtgaac ttcgcctttt cccacagtca gtttcacgcc attgagtgcc
     4081 ataacaccag agaaactttt tgtcagatct ttcacttcaa gtaagggagt agacatacaa
     4141 cctccgtaaa aaaacgccct ccggcctcag ccggaaggca gataaattat tcgctgattt
     4201 ctgcaaaacg ttctgcttct tgcagattat ctttcaggat aatttttggc ggatagtccg
     4261 caccgctcac ttctttgctc tcacggatat tgccaaccac ttgctctaat gcggtgctta
     4321 ccgcaaaact tgggcgttgg tcagcggtga gatacaacca gccatctttc acacgtgcaa
     4381 gagcctcagg ggttgagtca aagccggtta ctaaaatatc gccggctttc aaaccttggc
     4441 tacgcaatgc ttcaatcgca ccaagtgcca tatcgtcatt cgctgaaata atcacttctg
     4501 gtttctcttt taaggttggt aacacacttt caataatgcg taatccctca gaacggagcc
     4561 agttaccggt ttgatcaacc acgattttat atttgtcacc accggcagct aattcatcac
     4621 gaataccttt ggtacgttca atgttagagg ttgaacccgg ctggccggtt aaaagaataa
     4681 tttttgcccc attcgggtat ttggctttca ctgcttttgc gacttcttga ccgcctgtgt
     4741 agttatttgc cccaaagtgt ggaaccggct tactgctttc aacttttcgg tctaatgtcg
     4801 cagcaggaat tttttctttg ataatttctt ccactgcacc tgaaatcgca ttcacatcat
     4861 tcggggaaat gataatgcct ttagcaccac gggtaattgc attttctaag tcagaagcct
     4921 gcttagtaga agaactttgc ccgtctaata cttgcaattt gatctccaac tttttactgg
     4981 tttctaccgc tactttttgt agttgaactt caaacgggct ggataggttc ggtaagctaa
     5041 ataccacaag ctcatctttc gcaaagctgt tcattgctaa gcctaacgtt aaagcactca
     5101 atacaaattt aatacgcttt ttcattacaa aatcctcttt ttaagatggg aattgacggc
     5161 gaatattgcc aatgttactt caaactgcga aggaaactta agtcttattt caataataaa
     5221 cctcaatgaa ataaattttt caatttagaa tatatcattc aaaattttcc aaaccaaatg
     5281 ctttgtttca aatttgttac gtagatcaca tttttaggaa gagataaatg caagcggtca
     5341 aaacaggtcg cttttttgca aaaatttgaa taaaaatgac cgcttgtgag atgaaaacca
     5401 ataaaaaagc ggtgatttct caccgctaaa cactcaaaaa agaaatgtac tcgttattat
     5461 aaggttggga aattaaatgc cgcttggcta cgcaccgagc tgtccggcca acgggttgtg
     5521 atggttttca tacgggtgta gaaacgcacg ccgtctgtgc cgtacacatt gagaggcccg
     5581 aaaatagagg ctttccaacc gccaaagcag tggaacgcca ttggcacagg aattggaata
     5641 ttcacgccca ccataccggc ttgtacatct tgggtgaatt gacgtgctga atcgccatct
     5701 gcggtgaaaa ttgcactacc attgccgtat tggtgactgt taatgagtgc gatcccttcg
     5761 gcataatctt tcacacgcac aacagagaga accggtccga agatttcatc ttgccagatg
     5821 gtcatttcag gtgtcacgtt atcaaacagc gtacctccga caaagtaacc gttttcaaac
     5881 ccttcgactt tatagcctcg accatcgaca cagagtttcg ccccctgtgc caccccttga
     5941 tcgatatagc tctcaatttt ggctttatgc tctttggaaa tcaccggccc catatcattt
     6001 tcaagcacgc cttgtggtag taagcctgaa ccgattttca atgctttgac cttaggaatt
     6061 agctcttcaa tgattttatc ggctaattca tcaccaacaa ctaccgctac cgatagtgcc
     6121 atacaacgtt caccggctgc accaaatgct gcgcccagta aggcattaat tgtgccttgt
     6181 acatcagcat ccggcataat caaagcgtgg tttttcgcac cgcccaaggc ttgaacccgt
     6241 ttgccgtttg ctgagccttt ctcataaata tattgtgcaa ttgcagttga gccgacaaaa
     6301 ctcaccgcct gaatgcgaga gtcggttaaa agaatatcta ccgcttcttt atcaccttgc
     6361 accacattga atacaccgtc cggcaagccg gcttctttta ataattctgc caagcgaata
     6421 gacacacttg ggtctttttc agacggtttt aaaacgaaag tgttaccgca agcaagtgcc
     6481 actgggaaca tccacatcgg taccattgcc gggaaattga acggggtaat gcctgccact
     6541 acgcccaacg gttgctggat tgagtgaata tcaatgccac gccctgcatt ggcggagaac
     6601 tcaccttttt gtaagtgtgg aataccagtg gcaaattcca ccacttctaa accacgggtt
     6661 aattcgccaa tcgcatcgga atagattttg ccgtgttctg agctgattaa acgagccaac
     6721 tcatcaaagt ttgcttccaa taactctttg aatttaaata aaattctggc acggcgtagt
     6781 ggtgattgtt gtgaccagct tttgaaagcc tgatcggccg ctgcaattgc cgcatcgact
     6841 tcgtttggca ggcttaattt gacttttttg gtctcttcac cggttgcggg attaaatacc
     6901 gaggtaacac gctggctttg gctttcaacg actgcaccgt taataaaatt ttggacaaat
     6961 tggctcattg gaggctcctt atcaactgat tgagaggaaa aacggataaa atacagccga
     7021 taaactaccg ctaaaaatgg aaaatatcaa tacataatac taaaaaatga aaattttatt
     7081 tcatttttgt gatctaagta gcaagtttac agattgccgg tattaaaatg agacaaaatt
     7141 tcgtatagtt tgaaagcgta ttccatttcg ttaagcgagg tttcctatgt ctagtccatt
     7201 attgtctaaa cccaatttca accaacatga agggttaatt cagcacatta cccctgaaaa
     7261 tgcacagtgg gaatatgttg gatttgatgt ttatcagctc agtaagcaac aaattacgct
     7321 ggaaagtggt gaaaatgaac tttgtctagt ctttgtgacc ggttttggct ctgtctatgt
     7381 tgatgattat gcttttgaaa atatcggcaa ccgtaccacg ccatttgaaa aaattccgcc
     7441 ttatgcggtc tatatcccgc cacacaaaag cgtgttggtt aaaaccgaac tcaatttaga
     7501 attggcagtg tgcaaagcac caagtcaggg taattatccg gtgcgtttga ttagcccgaa
     7561 ccatatcggt gtggaaaaac ggggctttgg caacaatcaa cgcttggtgc ataatattct
     7621 gccggaaacc gaagcagccg acagtttgct ggtggtggaa gtctttaccg atgaaggcaa
     7681 taccagttcc tacccaagcc acaagcacga tgacaaacac tctaccgaag aaacctattt
     7741 ggaagagacc tactaccacc gcttcgatcc accacaaggt tttgcaatgc aacgggttta
     7801 taccgatgat cgcacacttg atgaatgtat ggcggtgtat gataaaaatg tggttaaagt
     7861 gcctcgtggc taccaccctg ttgctaccat tgccggctat aacaactact acctaaatgt
     7921 tatggcaggg ccggtacgca aatggaaatt tacttgggaa caagatcatc tttgggtgaa
     7981 cagtgaaacc tataaagcca aatttgagta atgcaagcgg tctttttcca ccaatttttt
     8041 acaaaataag ggattaaaaa tgggacgctt ttggcgtccc attcggttat actttcatac
     8101 aaagatattt tatttctaaa aactcttcaa taccgtactt cgaaccttcc cgcccaagtc
     8161 cagactcttt ccagccgccg aacggagcga tttcgttact gatgatcccc tcattaatcc
     8221 cgacaatgcc atattccaac tgctccgata ctcgccaaat tcgcccgata ttttggctga
     8281 ataagtaagc cgccaaacca aactcagtat cattcgccca ctggataacc tcttcttcgg
     8341 tttcaaattt gaaaatcggg gcaaggggag caaaggtttc ctcacgagcc actttcatcg
     8401 cttgagtgac atttttcaga atggttggct ggaagaatgt gccacccagt gcgtggcgtt
     8461 caccgcctaa caccagttct gccccaagtg aaagggcatc gttaatgtgg ctttcgactt
     8521 tttccaccgc tcgttcacta attagtgcac ctatcgtgac ctcttcctca aatggcgagc
     8581 caagcttgat ttgagccact ttctcactca attttgcaag aaattgctca taaatgcccg
     8641 cttgcacata aattcggtta gcacagacgc aggtttgccc accgttacgg aatttagagg
     8701 caaacacgcc ctcaaccgct gtatctagat ccgcatcatc aaacacaatc gccggtgcat
     8761 taccgcctag ctccaatgcc aattttttta ccgtccctgc cgattgttgc attaaaattt
     8821 tgccgacacg ggtggagccg gtaaaggtga gtttgcgtac ggtcggatta gtgctcaact
     8881 cccgccctac accttcgaca tcgctactgg taataatgtt aaatacccct ttgggaatac
     8941 ctgcttgatc cgctaattgg gctaatgcta aggcagaaag cggggtttct gccgccggtt
     9001 taatcacaat cgcacaacct gccgctaacg ccggtgccgc tttacgggta atcattgcat
     9061 ttgggaaatt ccacggtgta attgctgcca ccacgccaat gggttgttta gtcacaaaca
     9121 agcggcgacc ggctttatct tgtggcaaga catcgccata aatccgcttg ctttcttcgg
     9181 caaaccactc gataaaactg gctccatagg caatctcgcc tcgactttct gccaatggtt
     9241 taccctgttc tagacttaaa atctgggcga gttcttcttg gttttgcata atcagctcaa
     9301 accatttacg caaaatcacg gaacgctctt tgggcaataa tgcctgccat tttttctggg
     9361 caactgcggc tgcttcaatc gcttgagcgg tttcttccgt gcctaaatca gccacctcac
     9421 cgattttctc accattagtc ggattctgta ccgtaaaggt tttcccattt tggcttgaaa
     9481 caaatttacc gttaatatag gcttggaaac ggagtagcaa aaatttgtga tttggcataa
     9541 tcactccttg aatggttaaa aagagaacaa tattgactat aatccaattt cagtaaagag
     9601 aaaagagctg aaaaataact ttttactcaa taataaagca ccttagacaa gcgttcttct
     9661 aaggtgcagc catttattat tgagaattaa cttaaattct ctacatattt aataattaag
     9721 atacaatcat tggagcccat acgaatccaa aagcaagccc aacagcaacc ccaactaaag
     9781 ctggaagcat aaacggatga tctataatat atttgccagc acgagttgaa cccgtagtat
     9841 ctaattccat cgctgctaat gaagttgggt aggttggcag gataaaaatg cctgtaattg
     9901 caatgaaaga agccaaaatt gcccagttag ggatccccaa gcttactgct aatggaataa
     9961 taagagcaga agttgaccct tgtgaataaa gtaatgcaca agtaaaaaag agtactacag
    10021 ctaacaacat tggatattca gttaaaatat ttccaataga ggctttaatt tctattaaat
    10081 gtgcatcgac aaaagtcata cctaatgtaa caatcccgat aattaccgca atagaagtca
    10141 ttcctgagcg gaaaattgga ctagccacaa tcgtatcagg tttaattttt cctaccaaca
    10201 tcattaagca cgaagaggct aacatcatca caataattat atctcgggta ctcattgact
    10261 tcccattttc atatactggc tttaaccctg gaacggctgc aagtagaaca accaatacag
    10321 ttgctagtaa gaaaatgcca atcgatagtt ttgctccttt tgaataggta gattcactcg
    10381 ctaacttacg agatgggctg acgattcctg cttttaaacg ttctaaataa acaacatcat
    10441 tttgcaattc acagccctgc ttagaagcaa caaatgcagc aaccatagag gcaataaaag
    10501 aaacaggtaa gcatactgct attacttgaa aaaatgtaac actgttgttt tccattactg
    10561 ctatcatagc cgccattgca gcactcaatg gagaacctgc aatagctaat tgagaagaca
    10621 caaccgagcc ggctaatgct cttgaagggc gaataccact ctcttttgcc acttctgcaa
    10681 tcacaggtaa ggtggaaaag acgataaaac ctgtaccagc cataatcgtc attaaccaag
    10741 taatcatcgg tgcaataaag ttaatatatt taggattttt acgcatcaat acttccgcat
    10801 aacgaacaag taaatccata cctcctactg cttgtaacac agcagcacct attacaacag
    10861 tcataataat cagcattaca tcaactggaa tagaacccat aggtaaacca aaacctagag
    10921 ttagaatcgc aagccctaaa ccaccatata gtcctattcc taagccccca gaacgcagtc
    10981 ctaaatagat tgcagcaagt accacaaaga tttctgcata aatcatatcc atctccttaa
    11041 attaatgagt ttgataccaa tcaattgctt ttttaaccaa taccaacgtt gaatctacat
    11101 aacgcattgg ctcacgctct atttcttgag ctaaaataat aggtacttct gtacaaccta
    11161 aaataattac ttctgcacct aaacgaatga gttcatcacg ttgttgtaac attagctctg
    11221 tggctttctc taactcacct gattttaata aatagatact atccattact gccttttgac
    11281 cattttctgt cgggcaaata aaagtcagtc cttgtgcttc aagctgtttt ttgtataggt
    11341 ctaccgccaa agttgcatca gttgccaaca atccaacccg ccattttcca cacttcaaaa
    11401 cctcatcaac cacacaatca atcatactta gcatctctac ctgacttttg gccttcaagt
    11461 gttcaaacca ataatgtgca gtattacatg caataatgat gcaggttgcc cctgcactct
    11521 caagcccttc aatatatttt tccattaccg gagccggatt ctctccattt gctaaaatat
    11581 agcgagttct atctggtatg tcgggaatag aggaaatcaa taatggaata tgagcttgat
    11641 cacaatcagc tggtgttaag cggataaatt tttggaacat atctgctgtt gctgcaggtc
    11701 ccattccacc taaaatgcca ataatatttc ttgccataca cttctcctta taagatttaa
    11761 tttactgcga tgacttatac aaacaagtta tgcaaaaaag aaatgcaata aatcgaatca
    11821 gtatgcaaaa aatgcaacaa agctctttat agatagaaaa aaaatcttta aaatcaataa
    11881 aatactaata tatcaaatat aaatttaatg catttaatga aatcataatg cgaaatttgc
    11941 attggttagg atatgaaaaa catagaaaca aaatggttag aagatttctt aacgctagaa
    12001 gaatgtcgcc atttttctca ggcagcagaa aaaagaaatt tgtcacaatc tgcatttagc
    12061 agacgtattt tagctttaga agaagttgtg ggagtgaagt tatttgatcg tacctctatt
    12121 ccattacaac tcacagaaca aggtaagcta tttcattcac aaacgcgaaa cttattacaa
    12181 caattacaga ataatctaga tgaattatta ggacataatt gtaacttacc taatattaaa
    12241 tttgcggcgg ctcattccct ctctctttct attatgccca aattaattaa gaaattatca
    12301 caaacaaatg aaaattttat ttattccgtt gaa
//
LOCUS       DANLXQ010000074        11936 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.074, whole genome shotgun sequence.
ACCESSION   DANLXQ010000074 DANLXQ010000000
VERSION     DANLXQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11936
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.074"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            107..604
                     /gene="purE"
                     /locus_tag="PXD94_001516"
     CDS             107..604
                     /gene="purE"
                     /locus_tag="PXD94_001516"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL6286373.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            619..1584
                     /locus_tag="PXD94_001517"
     CDS             619..1584
                     /locus_tag="PXD94_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL6286374.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LAQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGVVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            1603..2691
                     /gene="purK"
                     /locus_tag="PXD94_001518"
     CDS             1603..2691
                     /gene="purK"
                     /locus_tag="PXD94_001518"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL6286375.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHTDKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(2912..3322)
                     /locus_tag="PXD94_001519"
     CDS             complement(2912..3322)
                     /locus_tag="PXD94_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286376.1"
                     /translation="MISRSLFSKLILFAKIVIFSQLVFMVVIWIAHHIMREYSNPFER
                     FGTLFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIWILGFIYFEPAV
                     YDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(3325..5343)
                     /gene="uvrB"
                     /locus_tag="PXD94_001520"
     CDS             complement(3325..5343)
                     /gene="uvrB"
                     /locus_tag="PXD94_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDL6286377.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGHSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIEMMNELGYCSGIENYSRYLSGRKEGEAPPTLFDYMPA
                     DGILVIDESHVTVPQIGGMYRGDRARKETLVQYGFRLPSALDNRPLRFEEFERLSPQT
                     IYVSATPGAYELEKNPDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDER
                     VLVTTLTKKMAEDLTDYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKA
                     ITETERRREKQMKYNEEHGITPQALNKKVGELLDIGQSDKPKRGKQAVKKAENSATDY
                     KPKSRKELEKELKTLEQQMRDFAKDLEFEKAAAVRDKLQHLKAYLLEV"
     gene            complement(5582..5818)
                     /locus_tag="PXD94_001521"
     CDS             complement(5582..5818)
                     /locus_tag="PXD94_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL6286378.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKRNV"
     gene            5981..6826
                     /locus_tag="PXD94_001522"
     CDS             5981..6826
                     /locus_tag="PXD94_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL6286379.1"
                     /translation="MFLLKGNFYAFNQTCFCTFALSGFFVATQAAALDLTKETEAYKQ
                     FVVEQIDQLVTDTEKFVGYLNKGDVQKAKKIYPLARMYFERSEPIAESFGDLDPRIDA
                     RLADLAEEGKTEKDWSGFHKIEKVLWQKNTTKGTKATAEQLLKDVKELRAKIPTAEVT
                     PEPMITGAVDLLNEVSTTKVTGEEEIFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDA
                     KLSTEIGSRFAAVNALLAKHNKSKTGYDYVPYNKLSKADIKALAEAVNQLGEPLAQLG
                     VLLNK"
     gene            6837..8033
                     /gene="efeB"
                     /locus_tag="PXD94_001523"
     CDS             6837..8033
                     /gene="efeB"
                     /locus_tag="PXD94_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL6286380.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELNDLPHFPRDQLQAEY
                     TGGDICIQACADDPQVAFHAVRNLVRVARGEIKMKWSQMGFNSFANQDTPRNLFGFKD
                     GTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWDRTHLQSQEETFGRHRD
                     SGAGIGHQHEFDKLDLAKKDEKGNAVIPEVSHAHLANKTGLKILRRSFSYSSGIDDKG
                     QFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGLFACFAGVKDENDYLGK
                     ALFDLI"
     gene            8044..8663
                     /locus_tag="PXD94_001524"
                     /pseudo
     CDS             8044..8663
                     /locus_tag="PXD94_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
     gene            complement(8699..9517)
                     /locus_tag="PXD94_001525"
     CDS             complement(8699..9517)
                     /locus_tag="PXD94_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL6286381.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTARWIRRLIADFKQAGGDGIEVSG
                     CGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIELGKSLELPQECKPIWTLF"
     gene            complement(9529..10128)
                     /locus_tag="PXD94_001526"
     CDS             complement(9529..10128)
                     /locus_tag="PXD94_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL6286382.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEAFLREKGVALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            complement(10125..11672)
                     /gene="sbcB"
                     /locus_tag="PXD94_001527"
     CDS             complement(10125..11672)
                     /gene="sbcB"
                     /locus_tag="PXD94_001527"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL6286383.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELPVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAAQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
BASE COUNT         3495 a         2604 c         2479 g         3358 t
ORIGIN      
        1 agataacagc cctaaaaatt gcttttcact tcttaaatag ctacgcaaac gtttgctttg
       61 tatgctatga ttacactgtt ttacattagt atcataagga tttaaaatgc caaataaagc
      121 tgaaattgcg atcgtaatgg gatctaagag tgattgggct acaatgtctg aagcaaccca
      181 aatattagac cagttcggtt tgagctatca cgttgaagtc gtctcagcac accgcacacc
      241 ggacaaactc ttttcttttg ctgaaactgc agagactaac ggctataaag tgattattgc
      301 cggagccggc ggtgcagctc acttgcccgg aatgatcgct gctaaaacca ttgtgccggt
      361 gttaggtgtg ccggtaaaaa gctcaatgtt aagtggtgta gatagcctct attcgattgt
      421 gcaaatgccg aaaggtattc cggtcggtac gctcgcaatc gggccggcag gtgcagcaaa
      481 tgcaggctta ttagccgctc aaattttagc agcctttaac cctgatattg cagagaaact
      541 gcgtattttc cgtcaaaccc aaactcagat ggtattagat aatcctgatc ctcgtgttga
      601 ataactattg tataaaagat gtctaaaaaa ttattaatcc tccacactgg tggtactatt
      661 tcaatgaaag aaggggaaga cggtaaggtc tccccttcca atgaaaatcc tctcttggct
      721 gccttagaac gtttaaacca cccagcaaaa ttggcgcaag aatcgttatt taatattccc
      781 tctccgcata tcaatatcga gcattggcaa cagttaaaaa tgcggattga aaaggcagtg
      841 aatgaggaga atgtcgatgg cgtagtaatc acccacggca cagatacctt ggaagaaacc
      901 tcctattttc tggatttagc cctcagcgta aatataccgg tagtaattac cggagcaatg
      961 cgttccagta acgaactggg tgctgatggc ttaatcaatc tacaaagtgc gattttagtg
     1021 gcgttaaatg aagaaagccg agataaaggc gtgttagtgg tgatgaatga tgaaatccac
     1081 aatgctaaat ttgtgaccaa aacccacacc actaatgtgg cgactttcca aaccccaacc
     1141 tttgggcctt gtggcttagt aacgaaagaa tctgtcattt ttttccaaaa attgaccact
     1201 tacgaacgtt atccaattca acaagtaact aaagctaacg tgcaactact gaaagcatac
     1261 gccggaatgg atagttttct gctggaacaa ttagcgaaaa gcaaatgcga tggtgttgtg
     1321 attgaggcat tgggtgcagg taatttaccc cctagttgtc tggctggtat tgaggcttta
     1381 gtgaatgcca atattccaat tgtaattgtt tctcgggcat tcaatggtat tacccaagat
     1441 gtctatgatt atgtaggcgg tggaaaacag ttaaagcaaa aaggcattat ttttaccaaa
     1501 ggcttaagtg ggcaaaaagc tcgcattaaa cttatggtct tactcagcca aaccttagac
     1561 aaaccactat cagactattt ctaacccatt gagaatacca cgatgcaaaa aagtgcaatc
     1621 tacccacctg tgtatgtatt aggcaacggc cagctcggga gaatgctacg ctacgccgga
     1681 gcaccacttg atattgaagt caaaccgctg gctttcgatg cccctgtgtt tgatattgag
     1741 ccaaacagca ttattaccgc agaaatcgaa cgttgggcag atacgccttt aacgcaatta
     1801 ctcggtaacc acccgaattt tgtgaacctg aatgtttttg ggaagttagc cgatcgtttt
     1861 acgcaaaaat cactacttga tgaactccat cttgcgactt caaaatggca actgctagaa
     1921 aatgccgaac attggcaaga tatttttcag catattggcg aaaaagtagt ggttaaacgc
     1981 cgaaccggag gatatgacgg acgtgggcaa tggattgtaa cgcaagactc gattcatcaa
     2041 attacacctg aattatttgg cgaagtgatt gccgaaaaat ttatcccgtt tgagggggaa
     2101 atttccattg ttggggcgag attccgtgac ggctcaaccc gtttctaccc tatttctcac
     2161 aatttacaac aaaacggcat tctacgttac tccgtctccg atgtgaattt accgaaccaa
     2221 gccctctacc agcagcaagc cgaacagatg ctcgctacgg taatgcaaaa attagagtat
     2281 gtcggcgtaa tggcaatgga atgctttgtt gtgggcgaca agctactcat caacgagctt
     2341 gcccctcgag tgcataacag cggacactgg acacaactcg gctgtgccat tagccaattt
     2401 gagttacatt tacgggcatt attagatcta cctacgccag cactcgacca aattgcccca
     2461 agcgtgatgg ttaatttaat aggcattgaa cataacaagc agtggctaag cctcccattt
     2521 agccaactac attggtatgg caaagaagtc cgagctggta gaaaagttgg gcatatcaac
     2581 ctcactcaca ccgataaaaa cggcttaatt gaactactgg aacaactcag accgagccta
     2641 acggaagatt tccattcggg attagattgg gcaatcaata agctcaagta agcggtcgga
     2701 ttttgtaact tttttgcaaa tctgtttacc cgatggtgcg agcttcctcg ctcgtaccaa
     2761 acaagcggtc gacttagtgc taaaatttgc aaaataattc aacataatgg ctacttccgg
     2821 tcatcatctt gatctgaaaa cccctcaggg tggatatggc tatcaaaaag cattttcttc
     2881 ctccgagaaa acagatttct tcacaagaat cttagagctg ctctttattt gtttccttag
     2941 gcaataactt cgagcaaatg ataatagaga gcatagcccc aaatcctgca cctgaaatct
     3001 tcagtgattt atcataaact gccggctcaa aataaataaa tcccaaaatc caaattaaaa
     3061 agaaccccat ataaatccaa aacactttat cttcattttt ctgggtataa cctttataaa
     3121 caataaccgc actacttacc atcatcggaa taataaaagt caaaaacaca ttattgaaca
     3181 gggtaccaaa tcgttcaaac ggattactgt actcccgcat aatatggtgg gcaatccaga
     3241 taaccaccat aaaaacaagt tgagagaaaa taacaatttt ggcaaataag attaattttg
     3301 aaaataatga acgagaaatc atatttaaac ctccaacaaa tacgccttta aatgctgcaa
     3361 cttatccctt accgcagccg ctttttcaaa ttccagatct ttcgcaaaat cacgcatttg
     3421 ttgttccagc gttttaagct ctttttccag ttctttacgg gatttcggct tgtagtcggt
     3481 tgcagaattt tcggcttttt tgaccgcttg tttgccccgt tttggcttgt cggattgtcc
     3541 gatgtcgagc aattcaccca cttttttgtt caacgcttgt ggcgtgatgc cgtgttcttc
     3601 gttgtatttc atctgctttt cacggcggcg ttcggtctca gtaattgctt tttgcattga
     3661 gttggtaatg cggtcgccgt ataaaatcgc ttttccgttg aggtttcggg cggcacgtcc
     3721 gatggtctga attaacgagc gttcggaacg taaaaatccc tctttatccg catcaagaat
     3781 ggcaaccagc gacacttcgg gcatatctaa gccctcacgc aataagttga tccccaccag
     3841 cacatcaaac attcccattc gtaaatcgtg gataatttcg acccgttcca ccgtgtcaat
     3901 gtcgctatgt agataacgaa ctcgtacgcc atgttcgtcc aaataatcgg tgagatcttc
     3961 cgccattttt ttggtgagtg ttgtcaccag cacccgttcg tccaccgcca ctcgtttgtg
     4021 aatttcagac agtaaatcgt ccacctgcgt agcaacgggg cgaacttcga taatcggatc
     4081 gagcaagccg gttggacgaa ctacttgatc caccacatcg ggatttttct ccaattcata
     4141 cgcccccggt gtggcagaaa cgtaaatcgt ctgtggtgaa agacgttcaa actcttcaaa
     4201 tctcaacggg cggttatcca gtgcagacgg cagacggaaa ccgtattgca ccaatgtttc
     4261 ttttctcgct cggtcgcctc gatacattcc gccaatttgt ggcacagtaa cgtggctttc
     4321 atcaatcacc aaaataccgt ccgccggcat ataatcaaac agggtcggag gggcttcgcc
     4381 ctcttttctg cccgagagat agcgtgagta gttttcaatg cccgaacagt agcccagctc
     4441 gttcatcatc tcaatatcaa actgcgtacg ttgggcgatc cgctgctctt ccagcaattt
     4501 attctctttg atgaaatagg tgcgacggtc ggcaagctcg accttaattt gctcgattgc
     4561 ctccaaaatc cgttcccgag gggtcacata gtgagttttc gggtaaatgg tatagcgagg
     4621 caccttgccc aagctatgcc ctgtaagcgg atcaaacagc gacagatttt cgatttcatc
     4681 gtcaaacagc tctaccctta acgctacttc atcgctttcg gcagggtaaa tatcaatcac
     4741 atcgccccgc actcggaaag tcgaacgttg gaacgcctga tcgttgcgag tatattgcaa
     4801 ttcggcaagg cgagagagaa tttcacgctg cccaatcatc gcccccactt gcaaatgcaa
     4861 catcatctgc atataagcgt ccacatcacc caagccgtaa atggcagaaa cggacgccac
     4921 tacaatcgta tctcggcgtt ccaaaaagga tttggttgcg gaaagtcgca tttgctcgat
     4981 ctgctcattg atagaggcat ctttctcgat aaaggtatcg cttgccggca cataggcttc
     5041 gggctggtag taatcatagt aagagacgaa atactccacc gcattttccg gaaagaacgc
     5101 tttcatctcg gcataaagct gggcggcaag ggttttattc ggggcaagca ccatcgccgg
     5161 gcgattgagc ttggcaatca cattagcaat ggtaaaggtt ttccccgagc cggttacgcc
     5221 aagtaaggtt tgatgtgcca agccgtcatt taagccttcg gtcaatttgg caatcgcact
     5281 tggttggtcg ccggagggct tgaaaggaga atggaggata aagggtttgc cttgcttgct
     5341 catagaaaat cctgtcttaa aaaatcggtt tattttagca caagcaagcg gtcgttttta
     5401 tcgggatttt tgcaaattta ttgtaactgc ttatattgat ccattcttgg atctaacacc
     5461 ctaacaataa agataccttt tgggattggt ttataataaa tagaatgtga gcggtggtaa
     5521 tgacagcgta acaaagggcg aataaagtaa cgctaaatct cgctctgccc taacagaaag
     5581 tttatacatt acgttttgcc ttgctgataa tagattgaac ggtttcctca gaaacaccgc
     5641 tttctaagcc ttcatcaatt aaggctctta gctgaattag ttgctgttct tgtgcttcca
     5701 atatttttaa tgcggcactg atgacctcgt tggtcgaacc ataacgccca ctttcaacca
     5761 tttggttcac aaattggctt aatggctcag ctaaagttac gctggttgtt ctactcatta
     5821 ctattcctat cttattgatt attgagcaga ttgtaatggc tctaatttag ataagcaagc
     5881 ggtctatttt tagcaataat ttgcaaaaca aaatgtgatt tacatcacaa aatcagcatt
     5941 attttttgcg aataattcgc aataaggata ttatgtcgca atatttttat taaaaggaaa
     6001 tttctatgcg tttaaccaaa cttgcttttg cacttttgca ctttctggct tttttgtggc
     6061 tactcaagcg gcagctttag acttaactaa ggaaaccgag gcttataaac aatttgtggt
     6121 agaacagatt gaccaactgg ttaccgatac ggaaaaattt gtcggctact taaacaaagg
     6181 cgatgtgcaa aaagcgaaga aaatttaccc gcttgcaaga atgtattttg aacgttctga
     6241 accgattgca gaaagttttg gcgatttaga ccctcgtatt gatgcccgtc tggcagattt
     6301 agccgaagaa ggtaaaaccg agaaagattg gtcaggtttc cataaaatcg agaaagtatt
     6361 gtggcagaaa aacaccacta aaggcacaaa agcaacggca gaacaattat taaaagatgt
     6421 aaaagagctg cgtgcgaaaa ttcccactgc agaagttacc cctgagccga tgattaccgg
     6481 tgcggtagat ttattaaacg aagtttctac tactaaagta accggtgaag aagaaatttt
     6541 ctctaaaacc gacctttacg attttaaagc caatattgaa ggtgcagaaa aaatctatga
     6601 gatttttaaa acgcaattag agcaaattga tgccaaactc tcaacagaaa tcggctctcg
     6661 ttttgcggct gtcaatgcgc tgttagcaaa acacaataaa tctaaaaccg gttatgatta
     6721 tgtgccttat aacaaactgt caaaagcgga tattaaagcg ttagcagagg ctgtaaacca
     6781 attaggcgag ccgttagctc aacttggcgt acttttaaat aaatagtgga gcaaaaatga
     6841 tggcagaaac cacttctcgc cgtgattttt taaaacatac cgcattagta ggagcaggct
     6901 tattgtctgc tcctgctttc gctttggaat cacgacaaat atctgaaaaa tctcaaaatc
     6961 aatacgactt ttacggcaaa catcaagctg gtattgttag ccctgcacaa aaacatatct
     7021 actttttagt gctggattta gacaccactg acattgccaa agtaaaaaca ttgtttcaaa
     7081 actggacgga atatagccgt aacctcacgc ttggcaaaaa tgtgaaacct tatagtgcta
     7141 atgcttatgt tccaccggcg gataccggtg aggcagatag cctttcggca cagaatctca
     7201 ctcttacttt tggcgtcagt gccaatttct ttacaaagct cggcattgaa aaacataagc
     7261 cgaaagaact gaatgattta ccgcatttcc ctcgtgatca actacaagca gaatacaccg
     7321 gtggcgatat ttgcattcaa gcctgtgcag acgatccgca agtggctttc cacgctgtgc
     7381 gtaatttggt gcgtgttgcc cgtggtgaaa tcaaaatgaa atggtcgcaa atggggttta
     7441 actcctttgc caatcaagac acacctcgta acttattcgg ttttaaagac ggcactgcca
     7501 acgcagaaag tttaaaagat caggaaaata cggtatgggt gcaacaagag ggctggctca
     7561 aaggcggttc ttatttagcc gttcgccgtg ttaaaatgtt cttggaaact tgggatcgca
     7621 cccatctcca atcccaagaa gaaaccttcg gtcgtcaccg tgattccgga gccggaatcg
     7681 ggcatcagca cgaatttgat aaattagatt tagccaaaaa agacgaaaaa ggcaatgcag
     7741 taattccgga agtttcacac gctcatcttg ccaataaaac ggggctgaaa atcctacgcc
     7801 gttcattctc ctattcaagc ggtattgatg ataaagggca atttgattca gggttgttgt
     7861 ttgtctcttt ccaacaaagt ccggagcaat ttatcaaaat ccagaacgct ctcggcaatg
     7921 tggataaaat gaatgaatac atcactcata tcggcagtgg tttatttgcc tgttttgccg
     7981 gtgtgaaaga tgaaaatgac taccttggca aagcgttgtt tgatttaatt tagggaaaag
     8041 gaaatgaaat taaaagcatt agtgctaatt ccgttactct tttcgagtac aggattgctt
     8101 gccaaagtga atgtcagccc cttatttgtt cagctttccg atgcaatggc agagagtaaa
     8161 aaaggggaat ttgcaaaatc cagccaaaat ttgaccgctt tacagcacga ttttgagcag
     8221 attagccagc acgattccct cgccggagtt gaagtacaga ccgctctgaa aaaagcgatt
     8281 gaaacaccca atgctgaaaa cttagagagc ctttctaaaa actctatgcg tttgaaaaag
     8341 aacaaaaccc tagcggttat gctgaaaaac atcaggattt tgtggcaaaa atgacgccgc
     8401 tttatgcaga acttgctcaa accgtgccaa ctcaagaaat cgccaaaatc aaatgggcgg
     8461 cttatcagtt tggtaagaat tgggtaaaac aggaaaaagc agtacgggaa atcagtcttc
     8521 cccattatgg caaatttgag cgtattcttg gtttaatgcg aattaatctg aacgctgaaa
     8581 agccggatat ggcaaaaatc agtgaactgg tttccgaact aggtgtggta atggcagact
     8641 ttaagcaagt taaagtgaaa tagtaaaaaa tcggggcaaa ttatttgccc cgataacact
     8701 aaaataacgt ccaaatcggt ttacattctt ggggtaattc caaactttta ccgagctcaa
     8761 tccagccagt tgggtagtgg aaatcagaac cgaccgagcc atataaatca aactcttttg
     8821 cccaacgggc aagtaattgc cgttgatcag gtgtttgtcc gcagcctgat acttcaatgc
     8881 catcgcctcc ggcttgtttg aaatctgcga ttaaacggcg aatccaacga gcagtcattt
     8941 tatagcgtag cgggtgagca atcgaaatca caccgcctga ggtgtgggta acattcaccg
     9001 cgtcttctaa actactccat ctcggtttca cataggcagg tttccccatt cccagataac
     9061 gcttaaaggc gtgttcaata ttgcggacat agccttgttc atacaaaaaa cgcccataat
     9121 gtgcccgagt tacttcccct gttgccaacg cttttgcccc ctcataggca tttgcaatcc
     9181 cgacttttgc taatttttca ccaatttcga ccgctctctc ttcccttaat tgggcttggt
     9241 tatccaaaaa cttcaccagc tcctcgttgt tttcatcaat attcaacgcc gcaagatgaa
     9301 tacttttttc ttgccataaa atcgaaattt ccaccccaga gataagctca agcggttgat
     9361 tttgggcaaa atttttcgct tcttgaatgc ccgaaatcgt atcgtgatca gttaaagcta
     9421 gcattgttac cccttgctct accgcacgtt gtaccaattc agtgggagaa agtacgccat
     9481 cagaggcggt actgtgggcg tgtaaatcat atttcatttt atttatcctt aaattaagcc
     9541 ggcagtcctg aaaaattggc gataggcttc ggttttgttg gctaagctga gtggataagc
     9601 cctcgacgtc gaaggcaggc gatataaata aagttctctt gtggcggaat aaggatagac
     9661 cacccattcg ttggttttcg gagctttgtg tttttccggc aataaaccga gcaacgtatc
     9721 ggttgccaaa ccacctgtgg taaatagcca tttcagtttg ggtaatttga gcaacagttc
     9781 gtccatatcg ataggatcaa caatttttaa atctttatct gaagcgtttc cttttaaacg
     9841 aatcgctgtt tttgccgaag aacagagcgc aacgcccttt tctcgcaaaa acgcctcaat
     9901 ccgaatcgga tcaaagcgtt tttcttcacc aactcgaaaa tagttagggt cagcataaaa
     9961 aatattgccc ataattcgcc acatatcatt ttgaaaattc gggtaatgaa attccataca
    10021 acgcttttcc gatgtaggtg gaaaagtgcc gaccattacc accgttgctt gttcaggtaa
    10081 aatagggtca aacgggtgct gttctagcgg taaattatcc attgtcattt aatcaactct
    10141 tttaacatct tgagcttttc ggttttatct aggggctgtt ccgctactct gttaagctcg
    10201 ctcagcaatt tttcatcttt tgcaacattt tgacgataaa tgaccgcttg ttgttgcgaa
    10261 atctgctcgg cgtaagcggt caaattttct aataatttta ccttttccgg gttgtcgtga
    10321 tgttgttgga acaagtcgtc aatcgattgg gcgaactgcg ctgcttttgc ctcaatttgc
    10381 tggttgcaat gtttctgcca tttgatctgc tcggctcgac tgagtgtttg tggatagtgc
    10441 cttgctcggt aatggaatag taacgcctct actcgagggt cttcaaattt caagccgtgg
    10501 ttggctaaat cttcaggcgg tagcgtgcgt aaaatcgcca tattattttt atccgcaggc
    10561 gagaaaaagc cgtcatacaa agtggtttct acattggtcg aaggctcaaa cgtgcgttct
    10621 tcattaaaaa tttcaatcac tttttcccga atcagcgatt tctgtgcttt taaagttttc
    10681 aaattttcca aacataaagc tctgtctatg cctaaacgtt ctgcattttc aggcagtaag
    10741 gtttttgccg gtgctaaaat cgggcattta ttaatatgca ctaattttaa cggcacaggt
    10801 aactcatttt cggctaattc atctcgtttg gtgtataaac gctgacgtaa aacctctgca
    10861 ttttcagtca ataagccatt catatcgccc gataaatcac acacaattac ggcattttga
    10921 ttagttgagt gccaagccag tggcacaacc caagcggtat ttcctcgata gttgcccagc
    10981 ataccggaaa cgtgcacaag cggtgtcatt tcaccagtgt caattaactt ctctacttct
    11041 tttttactgc gtaaattaaa gaaaaaatcg aacaactttg gctgtttttg cttaattaat
    11101 tttgccattg cgatggtggc atacacatca gacatcgcat cgtgtgcatt ttcatgggca
    11161 atgccattgg ctttggtgag gttttccaat ttaaaacttg gcataccggt ttcatctgtt
    11221 ggccattcaa taccatcagg tcttaacgca taacaggcac ggactaaatc taacaagtcc
    11281 caacgagaat ttccgttttt ccagctatat tcgtaaggat cgtaaaaatt gcggaagaag
    11341 gtataacggg tcatctcatc atcataacga atattgttat aaccgatagt acaagtattc
    11401 gctcggctaa attcagcgtg aattcgggct gcaaattccg gctcagatac accttcagcg
    11461 ttacattgtt gcggtgtaat cccggtaacc atcaccgctt cggggctggg taaataatcc
    11521 ggcgtttgct tacaaaaaaa cattaccggt tcaccgataa tattaaaatc cgcatcggtt
    11581 cgaataccgg caaattgtgc cggacgatct aacgccggac tcacgccgaa actttcataa
    11641 tcgtaaaaga aaaaagagaa tttatcgctc attcgttcaa tttgttagaa aaaattgggc
    11701 tgattgtagc acagtaaggc agaaaggagg gaatacaagc ggtcaatttc cgttagaaat
    11761 ttgcaaatgc taaaataaaa aaagccccta atcaaaaaag cgaaaagggg cggaggtctt
    11821 acctaatgat aatattattt ttataaatca attagttaag atgtttttgt cgtaaaaata
    11881 tcgagattat taaaaacacc tacaataaag caatacctca gcactcgaag agtaag
//
LOCUS       DANLXQ010000075        11735 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.075, whole genome shotgun sequence.
ACCESSION   DANLXQ010000075 DANLXQ010000000
VERSION     DANLXQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.075"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(97..172)
                     /locus_tag="PXD94_001528"
     tRNA            complement(97..172)
                     /locus_tag="PXD94_001528"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:His,seq:gtg)
     gene            complement(202..278)
                     /locus_tag="PXD94_001529"
     tRNA            complement(202..278)
                     /locus_tag="PXD94_001529"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(242..244),aa:Arg,seq:ccg)
     gene            complement(437..1159)
                     /gene="rffM"
                     /locus_tag="PXD94_001530"
     CDS             complement(437..1159)
                     /gene="rffM"
                     /locus_tag="PXD94_001530"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL6286384.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLVAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            complement(1149..2420)
                     /locus_tag="PXD94_001531"
     CDS             complement(1149..2420)
                     /locus_tag="PXD94_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HDL6286385.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTWANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            complement(2413..3483)
                     /locus_tag="PXD94_001532"
     CDS             complement(2413..3483)
                     /locus_tag="PXD94_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL6286386.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFPHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRTRSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            3670..5091
                     /gene="glnA"
                     /locus_tag="PXD94_001533"
     CDS             3670..5091
                     /gene="glnA"
                     /locus_tag="PXD94_001533"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL6286387.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            complement(5135..6278)
                     /gene="rffA"
                     /locus_tag="PXD94_001534"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /pseudo
     CDS             complement(5135..6278)
                     /gene="rffA"
                     /locus_tag="PXD94_001534"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
     gene            complement(6265..6909)
                     /gene="rffC"
                     /locus_tag="PXD94_001535"
                     /gene_synonym="wecD"
     CDS             complement(6265..6909)
                     /gene="rffC"
                     /locus_tag="PXD94_001535"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL6286388.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            complement(6906..8153)
                     /gene="wecC"
                     /locus_tag="PXD94_001536"
     CDS             complement(6906..8153)
                     /gene="wecC"
                     /locus_tag="PXD94_001536"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL6286389.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCSQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            complement(8194..9006)
                     /locus_tag="PXD94_001537"
                     /pseudo
     CDS             complement(8194..9006)
                     /locus_tag="PXD94_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
     gene            complement(9017..10081)
                     /gene="wecA"
                     /locus_tag="PXD94_001538"
     CDS             complement(9017..10081)
                     /gene="wecA"
                     /locus_tag="PXD94_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL6286390.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            10223..11503
                     /gene="hemL"
                     /locus_tag="PXD94_001539"
     CDS             10223..11503
                     /gene="hemL"
                     /locus_tag="PXD94_001539"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL6286391.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
BASE COUNT         3465 a         2742 c         2394 g         3134 t
ORIGIN      
        1 ggcgagatag gatttgaacc tacgacccac tggtcccaaa ccagttgcgc taccaagctg
       61 cgctactcgc cgtctgatac tcacaagagt attgaatggg gtggctaatg ggattcgaac
      121 ccacgacaac tggaatcaca atccagggct ctaccaactg agctatagcc accattgtgt
      181 tgatagtgcc gcattgacat ctggcgcgct cgacaagatt cgaacttgcg acctttggct
      241 ccggaggcca acgctctatc caactgagct acgaacgctt cgatttacat caattcaact
      301 taatgcgtcg caacggaggc gtatattata gatttcactt tcccttgtct agcacttttt
      361 aaaaaaaatg taaaaaaatg tattgtttgg ttttatttta ttcaaaacgt ttaataaata
      421 tcaaaaaatg cgttttttag agctgatttg tcagatagta ataggcatat tttagtaaat
      481 ttaattgtct ttgccagcgg gttggctgtt tcaatagtcg ataaagccat tccagcccca
      541 aattttgcca aattttaggt gctcgtttca ctttaccggc aaacacatcg taagtcccgc
      601 caacacccat atataaggct tgtggatatt gttgttgtaa ctgttggata aaaagttcct
      661 gttttggagt tcccattgcc acactaataa acttcgcgcc acttgcttta atttgctcta
      721 taacgtcctg ttcttgctcc gccttaaaat agccattctg cctaccgaca atattcacct
      781 tccattgtgc caattttgct tccaccttcg ttagggtatc cgccgagctg ccgaccaaaa
      841 acaccggcac atttaacgct ccggctcttt gcattaaggt ttcccataaa tcggctccgg
      901 caattcgctc tattttatag tttggatatt tttttcgaat cgattgaaca acactgatgc
      961 cgtctgcata gttgaactcg gcttcatcta tcactcggcg taacaacgga tttttttccg
     1021 acagaatcac tttctcagca ttaattgcca ctaatcttcc gaactgaatc cgcttttcat
     1081 tcatcagcca atttgcaaaa atttcccgat ttttgaccgc ttgtaacgca atgccccgaa
     1141 tgatcacttt atcgaacatt attatttttc cccattgaaa ggattttcgc cagcaaatag
     1201 caagccataa atatcagcgt gaaaaagcaa aaacgggaaa caaatgcatc gattccctct
     1261 cgcactaata cgatcaaatt gaataaattg ccgaagcagt aggcttgcaa caaggcggaa
     1321 ttgcacgctt tggcgtaagc gaataggtta tctaaggctt taatcatggc agctactatc
     1381 cccattccca acgccataac gctaaatccg cccataatat agaaagagcc gagtaaggta
     1441 ggcgaaatag ctaaccctga ttggttgcct aaaatcactt tggtaaaata attcgccgta
     1501 ttccagacga tctcagggcg ttcttgccat aaagattgtg ggatatatac atagaaatcc
     1561 cgcacaatcg gcattaaacc ttgatattcc acctcattag aaaatacacg agccaaattt
     1621 tcccaaggcg aaaaagtatc tcggcttaaa tataagaagg tgtaaatcat ctcgctaccg
     1681 gaaagggtaa gtttgtaacg ggctagagcg aggaagaaca tcgccaccac gccgattagg
     1741 cttacggcaa tcacctttga taacgacaga tagccttgct gcaatccaat caataagaaa
     1801 aagccaaccg ctagtgctaa atttgcccat gtgccgccca ctgccacata gcttaatatg
     1861 ccgaatgccc caccaacaat cagtaacgcc caccacgctg ttttattctt atataggaag
     1921 caaaaaatta acaacgccgg caataaaaag tagaaaaatc gttttaatgc cgaaagtatg
     1981 accctatctt tggcaaacag ttggctatat ttttcgagtt tgaaaagcag aaaaccgttt
     2041 aaataaagga aatatagcag ggcaacaact gccacagcgg caaggataca agcggtcgtt
     2101 tttgcctgaa aatttgcaac aagagcagtt ggttgcgaca cagcactcca caagcgtgaa
     2161 tagacaacgt aataaatcaa atagccaaca cctgctgccg taaaaacttg ccattgtgtt
     2221 tttagctccg gcagcgggta atcaaagcca aatgccatta ttaacgaaaa cggaaaaccg
     2281 gcgtaaaaag taacaaaata gagcaaacta aacagtaaat ggaaagaaat ccccgatttc
     2341 tgataaagtt gataaagtaa gctgaaaaat agcccgacag aaacaagata aaatccgcca
     2401 agtggtatta aattattcat tttgcattaa ttcgattaat cgctgttgcc atagtttagt
     2461 gtagcccgga gcaaaaaacg caatgcggtt tttatctatg ctctttagtt gttgtttggt
     2521 tgtttgaatc tgtgaagcag tcagattttc cgccaacaaa taaggcactt tttctgcctg
     2581 tagatccaac ccaaacggat tggctgaatg taacacacaa ggtacattat ggctaatcaa
     2641 aaggcttatc gtgccaatgc cttgctggcg tttaaaattg aaatagccga gatcacaatg
     2701 gctcaataac gtaacatact cttcaaagct cagcttcttc cgcaaaattt gcaaattttc
     2761 cgccaaaaat aaccgcttgc tataggcttc cacctgccga atatagcttt cattattttc
     2821 cgggtagccc attggtatga taatccgcac attgttccct agtcgttgat gaatctcgct
     2881 taaggcttga ataagctcat tcgctggatc gcctgaattg ccgagcaata tcgtcaagcg
     2941 atcattttgt gtggggctaa tttttatttg atcacctaat ttcgtgggga aatagattaa
     3001 atgatcctgt tctgatcttg tacgcatatt tcgccacaaa tgatccagat cgcctcttgt
     3061 tgcccaaacc tgtaagattt tttgttgggc aacacgccga aacgggtaaa acagcttaaa
     3121 tttccacgaa gttgccattt catataaatc cgccccccaa atatgccaaa tcaggttggt
     3181 ggtcggcaat ctgcctaagg ctatcgccag ccaaatccaa gggttaaact gcccgtgtaa
     3241 cagaaaacaa gcggtcggat tttgccgttt ttttgcaatt aaggcgttcg caatcgcttt
     3301 ttgtgaagag aactgcttaa tccgcaaatg aggaaaagca gtttgcagtg aaggcttgcc
     3361 taccacataa aaaaaatgtt cggagtctgg caataacggc aaaagctctt gctgaaaaaa
     3421 ggttaaaacc tgttggttat gatgctcaat ctcggagcct agaatatgat aaatcggcaa
     3481 cattacacgc ttttctctcc ccaccataca aacaatatgc aagcggtaag attttagcaa
     3541 aaatttgcaa cattactgca acttctttta gctttgaaac actaaaaaac atcacacact
     3601 tggttaaaaa atgctatgat aaccgagctt ttttattatt catttatccc aacgaggact
     3661 tattttacta tgtcaaacgc aatcgctaat gtatctaaac tgattcaaga caatgatatt
     3721 aaatttgtct tattaaaatt tactgatatt aaaggtaaag aacacggggt ttcgctccct
     3781 gtcagcttag ttgccaacga tttggaagac ttattcgagg aaggcaaaat gttcgacggt
     3841 tcgtccgtag aaggttggaa ggcaattaac aaagcagata tgttgttaat gccgattgct
     3901 gaaaccgctg tagttgaccc tttcgcccgt attcctaccc tttctattcg ttgttccatt
     3961 tacgacccaa acacaatgca gtcttatgac cgtgaccctc gctctattgc aacccgtgcc
     4021 gaaaactact tgaaatccac cggtatcgcc gatagcgtaa tgtttggacc tgagcctgaa
     4081 tttttcttat tcgatgatgt gcgttttagc accgaaatga acaatgtgtc ataccgaatt
     4141 gacgatgtag aagcggcttg gaataccaac cgtaaatttg aagaaggcaa caaagcctat
     4201 cgtccgttga aaaaaggcgg ctactgtgcg gttgcaccga ttgataccgc ccacgatatt
     4261 cgctccgaaa tgtgtttatt gatggaagag atgggtttag tgattgaagc tcaccaccac
     4321 gaagttgcga cagccggaca aaatgagatc gcaacccgat tcaacagcct gaccttaaaa
     4381 gccgatgaaa cccaaatcta taaatacatc gtgcaaaatg ttgccgtaga acacggaaaa
     4441 accgcttgct ttatgccaaa accgttcgcc ggcgacaacg gctcgggaat gcactgtaat
     4501 atgtcattaa gcaaagacgg caaaaatgtg tttatgggcg ataaatacgc cggcttatct
     4561 gaaactgccc tctactatat tggcggtatc attaaacacg caaaagcatt aaatgccttt
     4621 actaacccaa ccacgaactc ttataaacgt ttagtgccgg gttttgaagc accggtgtta
     4681 ttggcatatt ctgcaagtaa ccgctctgcc tctatccgta ttccggctgt gaccagtccg
     4741 aaagcgaccc gtgtagaagc acgcttcccg gatccaatgg caaacccata cctttgcttt
     4801 gccgccctgt taatggctgg cttagacggt gtgatcaaca aaatccaccc gggcgatgca
     4861 atggataaaa acttatacga tttaccgccg gaagagttaa aagatattcc aacggttgcc
     4921 tcgtctttag aatccgctct tgacgcatta agcaatgatt ttgaattctt aacacaaggt
     4981 ggcgtgttca cgaaagactt tattgatgct tatatcggta ttcgtcgcaa agaggtggaa
     5041 cgtgtaaata tgactccgca cccggtagaa tttgaacttt actattcata gtaacaacat
     5101 atagcaaaaa acggctgaaa cactcagccg ttttttattg attgaagaaa gcaagcactg
     5161 cttgaataac acgctcttgc tcctgcttac tcaaattata aaacagtggc aatcttacca
     5221 accgttcgct ttctaaggta gtaaagcgat catcacctgc taatcgacct aatttctgcc
     5281 ctgccggact gctgtgcaac ggcacataat gaaacacggt taagatattc tgtgccttca
     5341 taaaatcaat aaaagcagtt ctctgcttca agtcacttag ctttaagtag aacaaatgcc
     5401 cgttatggcg acaatattcg ccaatgcgag gaagctcgca cttagctgtt tgggcaagtg
     5461 gcaaaaaggc ttgatagtaa ttgtgccaaa gggttaaacg ctgttggttg attttatctg
     5521 cctctataag ctgagcatac aaataagccg cttgaatttc cgacattaaa aaacttgaac
     5581 caatatcctg ccagcgatat ttatctacct caccacgaaa aaattggcta cggttagtgc
     5641 ctttctctct gatgatttcc gctttttcca gcaaatgcct cattaatcac gatcgcccca
     5701 ccttcaccgc cagcggtata gtttttggtt tcgtgaaaac tatagcaacc aatatgcccg
     5761 atggaaccta acgccctacc tttataaaac gaattgactg cctgtgccgc atcttctacc
     5821 acataaagcc cgtatttttc ggctgttgcc attattgaat ccatatcaca agcaatgccg
     5881 gcataatgca ccggcacaat cactttggtt tttggggtaa tggcttgctc aattttgcgt
     5941 tcatcaatat tcattgtatc cggacgaata tccacaaaca caatctttgc cccacgcaac
     6001 gcaaacgcat ttgcagtaga gacaaaagta tagctcggca taatcacttc atcgtccggc
     6061 tgaatatcca gcagtattgc cgccatctct aacgcagccg tgcaagaagg ggtaagcaac
     6121 actttgctac gggccaggtt ttcaagccac tgctcacata agcgggtgaa tttaccaccg
     6181 cctgaaagct gccctgttgc catcgcctgt tctatatagg cttgctccgc ccccacaacc
     6241 gggggttgat taaacggaat aaaatcagga ttgttcatca tcgtattttt ccaaagcagg
     6301 gtaaaaccaa taatacaccg ccttaatgct tgcaccaaac gcttgataaa aacgaatcgc
     6361 tttctggtta ctcagctgag tggtaatcat cagttttttc gcattttgct gtttcgccca
     6421 cgctatcgca gcatttaaca attccgagcc aataccttgt tgtcggcaat caggctttac
     6481 cgccaataat ccgattttcg cttgctgttg ggcaagtaag cgaaccgtta ccgccccttg
     6541 aatttgcccg tcagcattgc attgaaccaa gccaatatca tcaaacttgc cttttaccgc
     6601 attttcaatc caagtccggt aaaagcgttg attctcttcc cttgaaaaat agggcgaacg
     6661 aaaccggcta tcgggaaatg ccgaaccaaa caataagttc aagtcgggca aatctgccat
     6721 atccgccggg cgacaagcgg tcatattttc agaaaatgtt gcaagtgcca gctcaaattc
     6781 caattctccc tccacaagta caaaaccttg tttttgcaac ctagcgattt gcctaaaatc
     6841 atcagccgct acttttactt gcacaatttc tctaccgaaa aaatcggaca gccattgatc
     6901 cggcttcatc ttacccacac tcctcgacaa tcaataatat gccgttgcct gatctgttcc
     6961 cttgaaattt gcttaaatga agtgtgatcc actaacaaga ctaaaatgtc tgcctgcgaa
     7021 agtgcggtgt tgagatcgac taaggtgatt ttttcgccag ccagctgttg aatatttggc
     7081 tcaaccgcca acaggctacc ctgatgccaa gcggcaagtt gccgggtaat cgctaatgcc
     7141 gggctttccc gcaaatcatc aatattcggc ttgaaagcta gccctaaaca agcgatggtt
     7201 agctcacttg gcttacaatc ttgctccatc gcacagtctg cgaccgatgt tttcacttgc
     7261 tcgattaccc attgcggttt gctgtcattc acttctcgtg ccgtgcgaat aaggcgagcc
     7321 tgttggggcg atgtggaaac gataaaccac gggtcaaccg ccaaacaatg tccgcccacc
     7381 cctgctccgg gctggagaat atttactctc gggtgaagat ttgccaattc aattagctcc
     7441 cacacattga tcttcaacgc atcacaaatc attgaaattt cattggcaaa ggcaatgttc
     7501 acatcacgaa agctgttttc caccaacttg cacatctccg ctgtgcgagc tgtggtggtg
     7561 acacaacgtc cttttgcaaa aatttggtat aaatcgaccg cttgttgact gcattgttcg
     7621 cttaatccgc cgataatgcg gtcattttca accaattccg tcattatatt acccggcaaa
     7681 acacgttcag ggcagtaggc aatagacaca tcggctggaa aggctaaatc agggcgagct
     7741 tggcttagcc attcggctaa attttctgtt gtaccgacag gcgaagtgga ctccagcacc
     7801 accaaattcc ctttacgcaa acaaggggca atcatagcgg cggcattttg aatataagtg
     7861 agatcgggct gcttcccgca aagtggggtt ggcacggcga tgataaaagc atcggccggt
     7921 tgcggtttgg cagttgcaaa aaaattacct tttttgaccg cttgtgtgaa ggcagtttgt
     7981 aaatccggct cgataatatg aatgttgcct tgattaaggc ttgccaccac ttgcggatta
     8041 atatccaccc caattacttg cttgccggca ttggcaaaaa cgactgaggt cggcaagccg
     8101 atatagccta accccagaat acaaatagtg ttaaattcag ttttactcat catcagttac
     8161 aaagagaaat ggcacactaa attgtgtgcc attttagatt aaagttccgc aggttgagct
     8221 ttcgctttca ccgcaaaact gagcataaag cccagcagta agccacttaa tgccccaatc
     8281 atcagccaaa ataggcggat caggcgattc tgatttaagt ggaacgctag gcgatttaac
     8341 caagcgataa gctgtaagtt ggttgtctaa cggctgaaca gaacgcatca tttgcagttt
     8401 agctgcccaa tcttgtgagc cttgcttacc gttaatattc aactccgaag cagttttaat
     8461 ttgctggaac aagactttcc atttcgcaat taaatcggcg tttaatttat ctcgagctta
     8521 ggtgtttacc aacggaataa aatcggctaa taatttttga gcctcttccg cattttctgc
     8581 aattaagctg acattatctg ccgaagttgc catatctttt tcaaaacgga attgttcaac
     8641 taattcagcc gccaccaccg ccggtgattt ctttgcaacc tgagctttta atttcaccgc
     8701 ttctgtttgt agcaaatagg cttgaagcaa gtcggcggac gtgaggttgc gtttaaattc
     8761 cgcataaacc tgctcacttg cctgttgctg tgccgtttgg ctattcgctt cttggctatt
     8821 actattcaaa aaatggtagg tggaaagtaa cgagtaataa ttacctaaat cgggaatagt
     8881 cggttgctca aattgagcgg tcactttcca tttttcaggt tgagtataac tcagcccata
     8941 accggcacct gccccgatag cagcaaataa aattagcact aaaagtcgat aaaataaggt
     9001 tttcacaaca tatcctttaa agttgtttgc tacgttttgc acggcgtttt agacggcgaa
     9061 caaaacgggt tacacgccaa gcgtgggtaa tggaataggc gtataggaaa aacagcccga
     9121 taaacattag catcatcacc cactgattcc aataatacac ctcgcctaat accccaatgg
     9181 tggaacaaaa tcccgcccag agggtaatca ccaataatgc ttggcgagag gtaagccctg
     9241 ctcgcatcat taaatggtgt aggtgcaagc ggtctggttt gaacggactt ttaccttttt
     9301 tcaaacggcg gataatcacc gcaaccatat caattaacgg cacggcaatc agccacaaac
     9361 cggtaatcgg gctaattggc gagccttgcc cttgggtgct taacagcaga atccaaataa
     9421 tggtaaagcc gatcagcata ctgcctgagt cgcccataaa cactttccat ttcgccccaa
     9481 acaggctcaa attgaacatt gcatacggca acaacacaaa gataagggca aagcaccaat
     9541 agcccaaact tggttcgcca ttaatgaaca ttaaagtgcc aagaccggca aaactcacgc
     9601 tggaaagccc tgctaataag ccatcaatcc catctaccat attaaacgca ttaataacgc
     9661 caatggtgat aaacacggta aagacaatgc cgagccaacc taattctata ctaaaggggg
     9721 caataatttg ccctaaactg ctgagcgaca agccactgta aatcatcgcc ccagctagtg
     9781 ctgcttgaat cccggcacgc agtaatgggc tgatgtcaaa tcggtcatct aacacgccga
     9841 taatcagcaa aatagttacc gctgtcagat acagttccgg cagccgcata tcttgccact
     9901 gcataaaata gaaagtgaga ttgccgataa ataatgcaat accaccgatt aacggaatca
     9961 acccctgatg gcgtttacgg aagttgggct tatctaccaa accaacaaat tccgccaccg
    10021 gacgcatcac aatcagggct aaaaaagaaa caataaatac ggttaaaaaa gtaagccaca
    10081 tacagggatt gcctcaaaaa atgagtcata aaataggaat gcagaatagc ataaggcaac
    10141 aaaatcgtaa attttttagc gaaaacaagg gcttttcagt acaataacag gcaaattttt
    10201 acatcaaaag aaaggaattg atatgtctat ttcagaacaa ctgtttgaac aagcccaaaa
    10261 agtgattccg ggcggtgtga actcccctgt gcgtgcattt aaaggcgtgg gcggcacacc
    10321 ggtttttatt gaaaaagccg aaggtgctta tattaccgac agcgacggca aacaatatat
    10381 cgactatgtc ggctcttggg ggccaatggt actcgggcat aaccacccgg cgattattga
    10441 tgcggtgcta aaagccgtgc caaaaggctt aagtttcggt gccccaacgg cagcagaaat
    10501 cacacttgcc gaattagtgt gcaaattagt accttctatt gaaatggtga gaatggtgag
    10561 cagcggaaca gaggcaacaa tgtctgccat tcgtttggct cgtggctata ccaaccgaga
    10621 taaaatcatc aaatttgaag gctgctacca cgggcattcc gattctctgt tagtaaaagc
    10681 cggttcaggt gctttaaccc tcggtcagcc aagcggcccg ggcgtacccg ctgatttcgc
    10741 caagcatacg cttacttgca cctataatga tttagattca gtaaaagcag catttgagca
    10801 atacccaaat gagatcgctt gtttaattgt tgagcctgtt gccggcaata tgaactgcat
    10861 tccgccacaa gaaggctttt tacaaggctt acgtgatctt tgcacacaat atggtgcggt
    10921 atttattatt gatgaagtaa tgaccggctt ccgtgtcgca ctcggtgggg ctcaatctta
    10981 ctatggggta acaccggatt taaccacact cggcaaaatt atcggcggcg gaatgccggt
    11041 gggggcattt ggtggtaaaa aagagattat ggaatacatc gctccaactg gcccggttta
    11101 ccaagccggc acgctctcag gcaacccgat tgcaatggct gcaggtttgg catgcttaac
    11161 tgaattatca aaagcaggaa atgaaaccat tttagcggaa aaaaccaaaa ccctcgctga
    11221 aggctttaaa gctcttgcag ataagcacaa tgtgccatta acggtacaat atgtcggcgg
    11281 aatgttcggc ttattcttca ccgaacaaaa agcagtaaac aacttccaag aagtaatgaa
    11341 atgtgatgcc gccaaattca accgtttctt ccacttaatg ctggaacaag gcgtgtattt
    11401 agccccatcg gcattcgagg cagggtttat gtctttagct cacagtgatg aagatattgc
    11461 tcgtacgcta gaggctgcgg ataaggcatt tgctcagctt tagaagaatg acaagcggtt
    11521 gggtttactc aataatttgc aagtttttga gggaatttga ccgcttgtag tgcaaatttc
    11581 tcttacgata gcaattatga tggtgttcgt ctctgacgag tgcttataca aactttcatt
    11641 atgcttaaac aagcacccgt ctggagacgg gcgctatcga gggaatagga ttgataaagc
    11701 aatatatggg cttccaacac ttgcaggcaa ggaca
//
LOCUS       DANLXQ010000076        11636 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.076, whole genome shotgun sequence.
ACCESSION   DANLXQ010000076 DANLXQ010000000
VERSION     DANLXQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.076"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            68..814
                     /gene="znuC"
                     /locus_tag="PXD94_001540"
     CDS             68..814
                     /gene="znuC"
                     /locus_tag="PXD94_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDL6286392.1"
                     /translation="MVSLENIEVSFNGQKALQNINLSIYPNSITTIVGPNGGGKSTLL
                     KILLKLLSPNKGKVIHQKGLKIGYVPQKLHLEPSMPITVYRFLALKPHTTKEMINQAL
                     DLFSITHLTENSMQKLSGGELQRVLLARAILDRPQLLVLDEPMQGVDIIGQTELYQLL
                     NKTRDLLNCAILMVSHDLNIVMANTDEVLCINKHICCAGSPEVITHDPSFIHFFGDQF
                     ARNVAFYSHKHNHHHNLHGDICLGECSCEH"
     gene            979..3792
                     /gene="sucA"
                     /locus_tag="PXD94_001541"
     CDS             979..3792
                     /gene="sucA"
                     /locus_tag="PXD94_001541"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL6286393.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQVEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDIEHEKVLAVTVHGDSAVAGQGVVQETLNMANVRGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            3895..5124
                     /gene="odhB"
                     /locus_tag="PXD94_001542"
     CDS             3895..5124
                     /gene="odhB"
                     /locus_tag="PXD94_001542"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL6286394.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            5417..6577
                     /gene="sucC"
                     /locus_tag="PXD94_001543"
     CDS             5417..6577
                     /gene="sucC"
                     /locus_tag="PXD94_001543"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL6286395.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQVHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVGVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            6586..7455
                     /gene="sucD"
                     /locus_tag="PXD94_001544"
     CDS             6586..7455
                     /gene="sucD"
                     /locus_tag="PXD94_001544"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL6286396.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(7526..8971)
                     /locus_tag="PXD94_001545"
     CDS             complement(7526..8971)
                     /locus_tag="PXD94_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL6286397.1"
                     /translation="MDSIILFFESVLTWIVNTIDGPLWDVTIILLLGAGLFFTLTTGF
                     VQLRLLPQSLREMWGGRSTEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAV
                     FWMWITALMGMSSAFAESSLAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTL
                     IFTFGFAFNAVQSNSIVEATKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVV
                     PMMALFYLIIAVIILGMNIEKVPSIISLIIESAFDFSAAAGGTFGALVSKTMLMGIKR
                     GLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGG
                     ETLRGISLTQSALQFHLGEFGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFV
                     WGFRAIVLFFVYFGAVRDGGIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQ
                     VKSGKEPVFRIEEHQDLIHKGVDPLIWKSKE"
     gene            complement(9076..10062)
                     /locus_tag="PXD94_001546"
     CDS             complement(9076..10062)
                     /locus_tag="PXD94_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HDL6286398.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLSFEKTKLNQAMKITF
                     ASALVQGLVAISLVTVIVVIFTLSRQYFNLTLKWVERGSFGLMILFGIYWCYQVYRTV
                     NRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQVRDWKAQL
                     MLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYLVIIARNK
                     AVKVSQWYFSAQINRNIVWILKFTLGLALILFGMVLFHSSFIDAGTGVFFKR"
     gene            complement(10053..10691)
                     /locus_tag="PXD94_001547"
     CDS             complement(10053..10691)
                     /locus_tag="PXD94_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL6286399.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNTSN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSLDMPENGDYSLGAQFAQKVEIICD"
     gene            complement(10752..11130)
                     /gene="rnpB"
                     /locus_tag="PXD94_001548"
     ncRNA           complement(10752..11130)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PXD94_001548"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         3645 a         2507 c         2304 g         3180 t
ORIGIN      
        1 tcatcttaaa ccgaatctaa ataccactac atttgaacta ttcaaacaga atttatccgc
       61 gacatcactg gtttctcttg agaatattga agtgtctttc aatggacaaa aagcattaca
      121 aaatattaat ttatctattt atcctaactc tatcacgacg atagtcgggc caaatggtgg
      181 aggaaaatct accctgctta aaattttgct aaaattactt agcccaaata aaggaaaggt
      241 tattcatcaa aaaggactca aaattggcta tgtaccacaa aaattgcatt tagaaccctc
      301 gatgcccatt actgtttatc gatttcttgc gctaaaacca catactacca aagaaatgat
      361 taaccaagca ctagatctgt tttctattac ccatctcact gaaaatagta tgcaaaaatt
      421 atcgggtggt gaacttcaac gtgtattgct ggctcgggca attttagatc gtccgcaact
      481 cttagtgtta gatgaaccaa tgcaaggcgt tgatattata ggccaaaccg aactctatca
      541 attacttaat aaaacaaggg atctccttaa ctgtgctatt ttaatggtct cacacgactt
      601 aaatattgtg atggcgaata cagatgaagt gctttgcatc aataaacata tttgttgtgc
      661 cggaagcccc gaagtcatta cacacgatcc gagctttatc catttttttg gcgaccaatt
      721 tgcacgtaat gttgcatttt attcacataa acataaccat caccataatt tacatggcga
      781 tatctgcctt ggtgaatgta gttgtgagca ctgataggcg tcctttttac caaatgttta
      841 aaatttgtaa aaataatgct aaaaataacc gcttgcagca caagatttat ctaaaaatca
      901 gacttacttc acattttatt tgcttaaatt ggagtataat aagggcgtga tttcatacca
      961 caaacaggtc aggtctttat gcaatataca cattttgatg attggcttgc atcatctgca
     1021 ttaggcggtg caaaccaatc ttatatagaa gaaatttatg aacagtatct agaaaatcca
     1081 gcaaatgttg atgaaagctg gagaaacatc tttgattcat ttcctaaaac tccccaagtt
     1141 gaacaagctc acacccctat tcgtgactat ttccgtaaat tagcccgtga acagcaacct
     1201 caatccgtca ctattattga cccagaagca ggggcgaaac aagttcgctt actgcaatgg
     1261 attaacgctc accgtttccg tggctattta gaggcaaaac tagacccaat taactattac
     1321 cgttggaaaa cctccagcgt gcctgaattg gactacaaat atcacggctt caccgatgaa
     1381 gatctcaacg aaacttttac ggtcggtcgc tatgtctatg ataaagacac aatgaagctc
     1441 agcgaactgg ataaagccct aaaagaaact tatttagggc ctattggttt agaatttatg
     1501 cacgttcaag atattgaaca acgcaactgg cttcaagcca aaatggaaag cgtgctaaac
     1561 aaaccgctgt tcaccaaagc agaaaaactg aatttattaa gcgaattgac cgctgccgat
     1621 ggtttagagc gttacttagg ggcaaaattc ccgggggcaa aacggttctc attggaaggt
     1681 agtgatgcct ttattccgat gatgaaagaa attatccgcc acgcttcccg tcaaggtatg
     1741 accgatgtgg ttttaggtat ggcacaccgt ggtcgtttga atatgttagt gaacgtattt
     1801 ggtaaacgcc cggcagaatt gtttgatgaa tttgccggca aacacgctga tgaaagccgc
     1861 acgggcgatg tgaaatacca ccaaggtttc tcctcaaact ttgccgtaga tgataaaaac
     1921 gttcacctta cccttgcttt caacccatcg catttagaga ttgttagccc ggtggtaatc
     1981 ggctcagtgc gtgcaagaca agaacgtatg cgtgatatcg aacacgaaaa ggtgcttgcg
     2041 gtgaccgttc acggtgactc tgcagtggca gggcaaggtg tagtgcaaga aaccttaaat
     2101 atggcaaatg ttcgtggcta taaagtagga ggcactatcc gtattgtgat caacaaccaa
     2161 atcggattta ccacctctaa cccaaatgac acccgctcca ccgaattttg taccgacatt
     2221 gccaaaatga ttcaggcacc gattattcac gttaatggtg atgatccgga agctgttgcc
     2281 tttgcagcga gaatggctgt ggaatatcgt actaaattta aacgtgatat ttttattgat
     2341 ttaatctctt accgtcgtca cggtcataat gaagccgatg agcctttagc aactcagcca
     2401 atgatgtata gcatcatcaa aaaacacccg acacctcgca aagtctatgc ggatcgttta
     2461 attaccgaag gtgttttaac caacgagcaa gaaatcgaga tgttaaacct gtatcgtgat
     2521 gccttagaca acggcgaatg cgttgtgccg gaatggcgtg aaatggatat ggcagcggtc
     2581 gattggctac aatatctcaa ttacgactgg actgccctat acgaaagtaa attccctgaa
     2641 gaacgattct taaccattgc caaacgggtg tgcgaatatc cggcttcgct taaagctcat
     2701 tctcgggttg aaaagatcta cactgatcgc aaagagatgt atgaaggcaa gaaattattc
     2761 gactggggta tggctgaaac aatggcatac gctactttgt tagacgaagg cacacatgtt
     2821 cgcctttcag gagaagatgc cggacgtggc actttcttcc atcgccatgc ggtaatccat
     2881 aaccaaaatg atggaacagg ctatgtgcca cttactcaac tgcacgccaa tcaagggcgt
     2941 ttccaagtat gggattccgt actctctgaa gaagcggtac tcgcctttga atatggcttt
     3001 gccactaccg acccgaaaac actgaccatt tgggaagctc aatttggaga tttcgctaac
     3061 ggagctcaaa ttgttatcga ccaatttatc agctcaggcg aacaaaaatg gggcagaatg
     3121 tgcggtttag tgatgttatt accacacggc tatgaaggtc aagggccgga acactcctct
     3181 gcccgagtgg aacgctattt acagctttgt gctcagcaaa atattcaagt ttgcgtgcca
     3241 tcaacaccgg cacaggttta ccatatgtta cgccgtcaag cgatccgcaa aatgcgtcgc
     3301 ccattggttg caatcacacc aaaatcattg ctacgccacc cacttgcggt gtcatctctt
     3361 gatgaattga ttaacggttc attccaaaat gtgattggcg agctcgataa tatcgatcct
     3421 aaacaagtca aacgtgtggt gatgtgttcg ggtaaagtgt actacgactt aattgaaaaa
     3481 cgccgtgaaa ataaccaaac ggacgttgcc attattcgta tcgaacagct ctacccgttc
     3541 ccacttgaag aaatgcaaca aattctcgag ccttacgctc acgttaaaga ctttgtctgg
     3601 tgtcaagaag agccactaaa ccaaggtgcg tggtattgca gcaagcacaa ttttgaggct
     3661 tctctgccgg aagatgcaaa attaacttac gcaggcagac cggcttctgc ttcaccggcg
     3721 gtgggttatc catcgttaca cgcaaaacaa caaaaacaac ttgttgaaga tgctttaacg
     3781 atcgctgaat aataaaatgg gcgtatatac gccctacaag cggttcactt ttaaccaaat
     3841 tttacaattt acgctgtcgg agttttaaaa ctctctcgaa aaaaggaata agttatgacc
     3901 attgaaattt taacccccga tttacccgaa tctgttgccg atgcgaccgt tgccacttgg
     3961 cataaaaaag tgggcgacaa tgtaaaacgt gatgaaattt tagtcgaaat tgaaacagac
     4021 aaagtcgttt tagaagtacc ggcttcaagc gatggtattc ttgcagaaat tacccaagaa
     4081 caaggtgcta ctgttgtatc caagcaatct ctgggtaaat tagtagtagc taaagccggt
     4141 gatattagct ctgcaactat tgagcaaaaa acagaatcca cgccatcaga ccgcaaacac
     4201 gcagcaattg aaaacagcca cgcgaacgct gatgatcaag ggcctgcgat tcgccgttta
     4261 ttagccgaac acgacttaca agccgctgat attcaaggca gtggtgtagg cggcagaatc
     4321 acccgcgaag atattgaaaa agagattgcc aaacgagtgc aaatcaacca agtaaaaggg
     4381 tctgcaacca gccaaaacac cgttagcact gtagcattta acagccgtag cgaaaaacgt
     4441 gtgccgatga cacgactccg caaacgtatt gccgaacgct tactagaggc aaaaaacacc
     4501 actgcaatgc tcactacttt taacgagctg gatatgcaac caattatgaa tttacgcaaa
     4561 acctatggcg aaaaattcga aaaacaacac ggtgtgcgtt taggctttat gtcgttctac
     4621 attaaagccg tggttgaagc attaaaacgc taccctgaaa tcaatgcctc cattgatggc
     4681 gatgatgtgg tttatcataa ctatttcgac atcagcattg cggtttctac cccacgaggc
     4741 ttagtcacac ctgtgttgcg tgattgcgac aaaatgagta tggcagatat tgagaagaaa
     4801 attaaagaac ttgccgaaaa agggcgtgac ggcaaattaa ccgttgaaga cttaaccggc
     4861 ggtaacttta ccatcaccaa cggcggggta ttcggttcat taatgtccac cccaattatc
     4921 aacccgccac aaagtgcgat tttgggtatg cacgctatta aagaccgccc tgtcgccatt
     4981 gacggacaag tagtgatccg cccgatgatg tacctagcct taagctatga ccaccgttta
     5041 attgacggta aagaatctgt cggtttctta gtggcagtta aagagttatt agaagaccca
     5101 acccgtcttc ttttggaaat ttaatcgaaa atatgaccgc ttgtactttt agctacaagc
     5161 ggtctgtttt taataaaatt tgacaaaatc cgataaacgc tctatttgag ttcccttctg
     5221 agccacctga gcgactttga atttgtagga aaaatttagt gtttgagctt acaaggcaag
     5281 cgagttataa atttttccgt caagcaaatt caaactaagc gaagaaagtg gcgaagcagg
     5341 gtgtgctttc ttttggttac ttttctttgc acaagcaaag aaaagtaaca taaaaaaaac
     5401 caaacgagga aactctatga atctacacga atatcaagca aaacaaatat tcaaacaata
     5461 cgggcttccc gtaagccaag ggattgcttg caaaacggca gaggaagccg ttgaggcaat
     5521 taaacaacta caaggtgaac aatgggcgat aaagtgccaa gttcacgctg gtgggcgtgg
     5581 caaagccggc ggggtgaaac tggttcgcaa tgaagccgaa gtgcgtgaat ttgccgataa
     5641 atggctcggc aaacgcttag ttaccttcca aaccgatgct aatgggcaac cggttaatac
     5701 cttatattta gaagaaacct gcaatatcga ccgtgaattg tacttaggtg cagtggttga
     5761 tcgctccgct caaaaagtga tttttatggc atcaagtgcc ggtggtatga atattgaaga
     5821 agtagcggag aaaacccctg aattaattca caaagcggta attgacccac tcaccggtgg
     5881 gcagggttat caagggcgtg aattagcatt taaattaggc ttgaaaggtg atgaagttaa
     5941 acaatttgcg gatattttcg tgaaactggc taacctattt gttgaaaaag atttagcttt
     6001 attagaggta aatccacttg tcatcactaa agaagggcaa ctactctgct tagatgcaaa
     6061 aatgtcgatt gacagcaatg ctctctaccg ccacccagaa cttaaagagt tgcaagaccc
     6121 atcgcaagat gacgaacgtg aagctgaagc ggaaaaatgg aatctcaact acgttgcact
     6181 agatggcaat atcggctgta tggtaaacgg tgcggggctg gcaatgggga cgatggatat
     6241 cgtcaaactt tatggtggta aaccggctaa cttcttagac gttggcggtg gtgcaaccaa
     6301 agagcgtgta gcagcagctt ttaaattgat tttatctgat ccgaatgtga aagccgtgtt
     6361 agtcaatatt tttggtggta tcgtccgctg cgatttgatt gctgatggcg tcattgctgc
     6421 tgtaaacgaa gtcggcgtga atgtgcctgt tattgtgcgt ttagaaggca ctaatgccgc
     6481 acaggggcgt gagattttag ccaatagcgg gctgagtatt ctagcggcat catcattaaa
     6541 acaagcggct gaattagcgg taaaatctgc aaattaggag gaaaaatgtc gattttaatt
     6601 gataaaaaca caaaagtaat ttgccaaggt ttcaccggcg gacaaggtac tttccacagc
     6661 gaacaagccc ttgcttatgg cacacaatta gtcggcggag tttcgccgaa taaaggtggc
     6721 acaacccatt taggcttacc ggttttcaac accgtacgcg aagcggtaga agcaaccggt
     6781 gcaactgcaa ccgttatcta cgttccggct cccggctgta aagatgctat tttagaagcc
     6841 attgatgccg gcatccaact aattgtctgt attactgaag gcattccaac cttggatatg
     6901 ctacaagtta aacaacgtct aaaccaaacc ggcgtgcgaa tgatcggtcc gaactgcccg
     6961 ggtgtgatta cccctgatga gtgcaaaatc ggcattatgc cgggcaatat ccacaaaaaa
     7021 ggcaatatcg gtattgtttc tcgttcgggt actttaacct atgaagcagt taaacaaaca
     7081 actgacgaag gctttggtca atctacttgt gtcggtatcg gtggtgatcc gattccggga
     7141 tctaacttta ttgacatttt aaaactgttc caagacgatc ctgaaactga agcaattgtg
     7201 atgattggtg aaatcggcgg ctctgccgaa gaagaagcgg cagctttcat caaagaaaat
     7261 gtcaccaagc ctgtagtcag ctatattgcc ggtgtcaccg caccaaaagg caaacgaatg
     7321 ggacacgccg gtgcgattat cagcggcggc aaaggaaccg ccgatgaaaa attcaaaatc
     7381 cttgaagatg ccggtgtgac aaccgtcaga agtttagccg aaatcggttc agctcttaga
     7441 aaaattttag gctaaactaa aagttcaagc agtcatattt acccattttt ttgtaaatat
     7501 gaccgcttgt ttttttgcta ttaaattact ctttcgattt ccaaattaat gggtcaacac
     7561 ctttatggat aaggtcttga tgttcttcaa ttctaaacac tggctctttg ccacttttca
     7621 cttggcgaag atagtctttt aaaataatcc agacaatcgg agcaagaatt aagatagcca
     7681 ccaagttaat taccgccatt gatgccatca ctgtatcggc aaatgcccat acaatccctc
     7741 cgtctcttac tgcaccgaag tacacaaaga ataaaacgat ggcacggaat ccccacacaa
     7801 accacggact gtttttaatg tagcggatat tagtttctgc ataagcatag ttaccgataa
     7861 tggatgtgaa gcaaaacagt aaaatcacaa atgctaaaaa gtgtaaacca aattcaccta
     7921 aatggaattg taacgcactt tgagtaagtg aaatacctct taaagtttca ccaccatagt
     7981 tatttgacat aagaatgatc actgcagtac aagtacagac tacaattgta tctacaaata
     8041 cgcccaacat ttgaattaag ccttgacttg ccgggtgttt cgcatcagaa gtcgctgcag
     8101 aatttggtgc agatcccata ccagcttcgt ttgagaataa gccacgttta atccccatta
     8161 gcatagtttt ggataccaat gctccaaacg ttccacctgc agcagcagaa aaatcaaagg
     8221 cgctttcaat aattaaagag attatacttg gcactttttc aatattcata cctaaaataa
     8281 ttactgcaat aatcaagtag aataatgcca tcattggaac aacttgggca gaaactttac
     8341 caatacgctt gacccctcca gaaataatta accctgtaat caccactaat gtcatgccta
     8401 cgtagactgg gttccaatcc cacgcatttt tggttgcttc cacaatagag ttagattgca
     8461 cagcattaaa agcaaaacca aaggtgaaga taagcgtcac tgcaaaagca atagcaagag
     8521 aaggcagttt taaaccacga gtgatgtaat aggctggccc accacgaaac attccgtttg
     8581 gatctttggt tttatacaac tgagccagtg aagactcagc aaaggcggaa ctcattccca
     8641 ttaaggcggt aatccacatc cagaataccg cacccggccc acccaaagcg attgcagtag
     8701 ctaccccacc aatattacca accccaacac ggctggctaa tccagtagta aaggcttgga
     8761 acggagttaa tgatttccct tccgtagaac gtcctcccca catttcacgt aaactttgcg
     8821 gaagaagacg cagctggaca aaacctgttg taagagtgaa aaataaccct gcaccgagca
     8881 ataggataat cgttacatcc caaagtggac cgtcgattgt gttcacaatc caagtcagta
     8941 cactctcaaa aaatagaata attgaatcca taaagacctc ttaattgaaa ataaaacttc
     9001 cacattgtaa ccgatttaat atttttaaaa agttttttta atacttttaa taaaaatttt
     9061 taataactta ttaatttagc gtttgaaaaa aacgcctgta ccagcatcaa tgaaactact
     9121 gtgaaataat accattccaa atagaattaa tgcgagtcca agtgtaaatt ttaatatcca
     9181 aacgatattt cgattaattt gagctgaaaa ataccattgg ctaaccttaa ctgccttatt
     9241 tctcgcaatg ataacaagat aagcaaataa gcttaaagtc agccctgttc ctactgccat
     9301 agctaatgct gaaataaccc cccaagcata gagattgagc gtataagaaa gaaataaaac
     9361 aagaatagcg ccagagcaag gtcttgagcc aatacttaat accaacatca gctgtgcttt
     9421 ccaatctctc acttgttgca tttcatttga agtcggtaaa tgtttatgcc tacagctaca
     9481 attttcggta tgaatatgcc ctttctgcgt gacaagcggt aattttccag cttttttttg
     9541 cgaaatacga atggatttaa tgataggctt tcgcgctctg ttcaccgttc gatacacctg
     9601 gtaacaccag taaatgccga acaagatcat caaaccaaag ctcccccgct caacccattt
     9661 tagggttaaa ttaaaatatt ggcgagagag agtaaaaatc accacaatca ccgtcaccaa
     9721 tgaaattgcc actaagccct gcaccaatgc tgaggcaaaa gtgattttca tcgcctgatt
     9781 taattttgtt ttttcaaaag aaagataact gctcagaata aattttccgt gaccaggccc
     9841 aactgcgtga aatactccat ataaaaaact aataaatact aaggtaatac cggctttact
     9901 ttgattttca gctaattcat tgagcgaagc ggaaagctgt aaattaaagt ttttttgcca
     9961 ttccatcact ttaaacaata tcattggata aagctgataa agagcaaata ggctcaaaaa
    10021 tcctatcact atcattcccc aatatgttgc ttttaatcgc atataatctc cactttttga
    10081 gcaaattggg caccaagaga gtaatctcca ttttcaggca tatccagtga ttggttttta
    10141 tccaaaccgg aagcatataa gcgtaactta ctatctactt gaggttgcac caatgttgct
    10201 ttacaacttg cattactggt attgacatca gcagggcgat tatattccat cgcaatataa
    10261 taactcggtt cataagtata gaatctcact ttactctgct ttaattcgtg aggtttagct
    10321 aaatgaaaag taatataaaa aatcactctg ttattcttga tttcaaagcg agtcgctgtg
    10381 ggctttgtcg taaattttat cggctgattt tgactatcat ataaggtgct aaaataatga
    10441 ttttgcaatg cggtttgcac catttctgca gtgatattct gctgagtttt ttctttatct
    10501 tggctatttt tcacttcata aattaactca gaagaagcaa tttcatctag catccattcc
    10561 atttggaaag atttcaacat tgagccttcc acaataacat tcgttttcaa atcaacaaat
    10621 gaatgtgggt gagaaaaaac tggcattgaa tataatgcaa ttaacgtgat aatcgtctta
    10681 ataaatttca tatttacttt cactataaaa taaaaaagcg aacaagttcc ccctgttcgc
    10741 atttcaagaa tttagtgagt tggtcgataa gccgagttct gtcgtggaca atcattcctc
    10801 tagacgtaca tttacacata cgctcaagca acctacccga actctcagcg ggccaccgat
    10861 tgagttccta tttggtcttg ctacgagtgg agtttaccct gctgtggaat gttaccaccc
    10921 acacggtgcg ctcttaccgc accctttcac ccttaccgac aaagccggcg gtctgctctc
    10981 tgttgcactg gtcgtaggct cacgcccccc ggacgttatc cggcactctg ccctgtgtag
    11041 ctcggacttt cctctcgttt acttgtcccc cagtttacgt tttcacgaat ttagaggctt
    11101 caataaacca gcgattgcct aaccaactca gcactagagt ataatgcttt cgcaaaaaat
    11161 tgcaaggaaa taaccgcttg tagttattat ggcaaaataa aaaaggatta agttcattta
    11221 aaaacttaat cctcttcgct ttaattcact cgtaaaaatt acgcacgttt gaagtctaag
    11281 tgaactaatt ttggtttctc tattttatta caataatttc aaagggttat ttaatcaatt
    11341 tcctttctat ttccgaaact atatacattt ttccgaaact caaattttaa tcagattata
    11401 aaacaagcta ttttcctttt ttctcattcc aaaaatacca tccaaaacat cttaccgcca
    11461 accaagtgct ccacgcaaaa ataggtctat tttgcgcaat caaacaatcc cttaaccgct
    11521 tatctgcttc cgctctactg acttttccgt ttacgcccac gatcgtgttg atgacagcac
    11581 gaattaatat tttcaggggc gtgtaccgca atcaatttta attataacca attgtt
//
LOCUS       DANLXQ010000077        11574 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.077, whole genome shotgun sequence.
ACCESSION   DANLXQ010000077 DANLXQ010000000
VERSION     DANLXQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.077"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            103..1995
                     /gene="mnmG"
                     /locus_tag="PXD94_001549"
     CDS             103..1995
                     /gene="mnmG"
                     /locus_tag="PXD94_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL6286400.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLILTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNNSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINAA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQVAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            2090..2314
                     /locus_tag="PXD94_001550"
     CDS             2090..2314
                     /locus_tag="PXD94_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286401.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            2327..2941
                     /gene="rsmG"
                     /locus_tag="PXD94_001551"
     CDS             2327..2941
                     /gene="rsmG"
                     /locus_tag="PXD94_001551"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL6286402.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPTENGKF
                     YALKGVYDEQEIIEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            3209..4657
                     /locus_tag="PXD94_001552"
     CDS             3209..4657
                     /locus_tag="PXD94_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6286403.1"
                     /translation="MNRVTNKADEFAKLSTFERIAYGSGDLAQNLVFGTVGGFLLTYL
                     TTVNGISAVAGATIFLIVKWGNVFWDPFVGAFIDKSKPSAQGKYRPYLLKFGIPLVVL
                     ASLLFLPLESVRGSVIYAFLSYFATAMVYSFVNIPYGSLSASLTRDTDEIAKLTTTRM
                     TMANIANLFVYTLFPLFVQLLAPHAELKNTGFFGIELYLGDYMSAAAGPAWFKVYAVY
                     MVIGFVALMLSYFGTKERIIPTAEETDKVKYSDLVNELKRNRPLQILGLFFLIGFTFM
                     FFGNTVWPFYLKYNIGHSEWIASVGLLGSIPGIFLVFLWPKLRRTIGKKNFFYLFLGI
                     FVLGQLCLWVWQFDSFKYSPTLGYIGRFLQQWGLTSATGFMWALVPEVVTFGEFKSKK
                     RVAGIINALMGLFFKIGLALGGIIPGYINAVYGFNGSLLAQTEEALFGINLSMIWAPI
                     ILAIIAGFVMSRYPLSDTDIDHINEKITAYSK"
     gene            4680..5726
                     /locus_tag="PXD94_001553"
     CDS             4680..5726
                     /locus_tag="PXD94_001553"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL6286404.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            5729..6367
                     /locus_tag="PXD94_001554"
     CDS             5729..6367
                     /locus_tag="PXD94_001554"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL6286405.1"
                     /translation="MPFSKEMGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAE
                     TKVKFTPFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYL
                     KDKAIVIPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYG
                     GFFTGAFVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            6386..7366
                     /locus_tag="PXD94_001555"
     CDS             6386..7366
                     /locus_tag="PXD94_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL6286406.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETAEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNRLIRPSVHHLW"
     gene            complement(7428..8768)
                     /locus_tag="PXD94_001556"
     CDS             complement(7428..8768)
                     /locus_tag="PXD94_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6286407.1"
                     /translation="MFSFLKASPPAEQKVEASRVDAEYRKLRWQVFAGVFIGYAAYYL
                     IRKNFSLAMPYLIDEYGFTKADLGTVGVALSLAYGFSKFIMGNVSDRSNPKYFITIGL
                     LGSAIVSLVFGLVPGVLSSIPIMIILAALNGWFQGMGYPPGAKTMTNWFSTSERGVWW
                     SWWNVSHNLGGGLIGPLAILGLSIFGVWQSLFYLPALIAIVLAFVMFYLMRDTPESQG
                     LPPIDEYKGEKVVEKVESAHTLSSKDIFYKYILNNKFLWAIAIANVFVYFIRYGIIDW
                     APTYLKEVKHFSVDKQSWAYFLYEYAGIFGMLASGYLSDKVFKGHRAPPMLLFLAGVL
                     IAIVVYWKNPAGNPLIDNICLVAIGFLIYGPVMMIGLQAADLVPRVATGTATGLTGLF
                     GYLLGSASAGYVMGKLVDMFGWDGGFYALILSCFLAFAFIALTLFKKTSKQLEV"
     gene            complement(8958..10031)
                     /gene="glpQ"
                     /locus_tag="PXD94_001557"
     CDS             complement(8958..10031)
                     /gene="glpQ"
                     /locus_tag="PXD94_001557"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL6286408.1"
                     /translation="MKFKFKTLVAALALSATALGAYAQQSDKLVIAHRGASGYLPEHT
                     LESKALAFGQQADYLEQDLAMTKDNRLIVIHDHFLDGLTDVAKKFPERKRADGRYYVV
                     DFTLDEIKTLEMTENFEVKDGKQAQVYPNRFPMWQSHFTIHTLEEELEFIQGLEKSTG
                     KKIGIYPEIKAPWLHHKEGKDIALETLKVLKKYGYDSKTSPIYLQTFDFNELKRIKTE
                     LLPKLGMDVKLVQLVAYTDWHETEEKDKNGNWVNYDYDWMFKDGAMAEVAKYADGVGP
                     GWYMLVDKEKSKVGNIVYTPLVKELAKYKMELHPYTVRKDALPDFFQNVDEMYDALLN
                     KAGATGVFTDFPDTGVEFLKKQK"
     gene            complement(10101..11546)
                     /gene="glpT"
                     /locus_tag="PXD94_001558"
     CDS             complement(10101..11546)
                     /gene="glpT"
                     /locus_tag="PXD94_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL6286409.1"
                     /translation="MFGPFKPAPHIAELPADKVDARYKFLRWQVFAGIFFGYAAYYFV
                     RANFDLAQKGLIEAGMYTKTDLGVIGTAAGLAYGLSKFVMAGMSDRSNPKVFLPTGLL
                     LSGLCMTLMGLMPWATSSILVMWVMIFLNGWFQGMGWPPCGRTMVHWWSKSERGTIVS
                     IWNTAHNLGGMVPGAMVLLASALYFSEYGVEATAKDVWQQALYFPGIAAMIAAIPIYL
                     VMKDTPQSCGLPPVEKWRNDYPDDYNEKTYEHDLSTKEIFVTYVLKNKLLWYIAIANV
                     FVYLIRYGVLKWSPVYLGEVKHFNIKGTAWAYTIYELAAIPGTLLCGWVSDKLFKGKR
                     GLTGFIFMILTTAAVVAFWLNPATPESEIAQYAGHAWYENPYQLMDFIWMTTIGFLIY
                     GPVMLIGLHALELAPKKAAGTAAGFTGLFGYLGGTVAASAVVGWAADKFGWDGGFYVM
                     IGGGVLAILLTFIVMVEEGKHKAKLEDHYGK"
BASE COUNT         3326 a         2443 c         2489 g         3316 t
ORIGIN      
        1 ttagatctta acctctctaa aaatctattt cccagatgat ccctttttac gctaaaatat
       61 gcgttcattt ttcagcagaa tttgtaagat agtagaagag atatgattta caaccaaatt
      121 tacgatgtga tcgtggtcgg cggtggtcac gccggcacag aagcggccct agcaccggca
      181 cgaatgggct taaaaaccct gattttaacc cacaatgttg ataccttagg gcaaatgtcg
      241 tgcaacccgg ctatcggagg gatcggcaaa ggccatctgg ttaaagagat cgatgcgatg
      301 ggcggtttaa tggcaaaagc aacagacgtt gccgggatcc aattccgtac cctaaacaac
      361 tcaaaagggc ctgcggtgcg tgccacccgt gcccaagctg accgtgtgct ttaccgtaat
      421 gcggtacgca ctgcgttaga aaatcaacca aatttagata ttttccagca agaagtagtg
      481 gatattttgg tggaaaacaa ccgtgcagtg ggtgcagtta ccaaaatggg cttaacgttt
      541 aaagcccgtg cggtagtttt aaccgctggt acgttcttag cgggtaagat ccacattggg
      601 ttggataact atgcaggtgg tcgtgccggc gatcctgctg caacaatgtt ggcagatcgt
      661 ctgcgtgatc tgaatttgcg tgttgatcgc cttaaaacag gtacaccacc tcgcctcgat
      721 gcccgtacca tcaactttga cgtacttgcc aaacagcacg gcgatgaggt gttacctgtc
      781 ttctctttta tgggcgatgt ctcacagcac ccacgccaaa ttccgtgcta catcacccac
      841 actaacgatc aaacccacga tttaattcgc aacagtcttg atcgcagccc gatgtacacc
      901 ggtattatcg aaggggttgg tccacgttac tgcccgtcta ttgaagataa agtaatgcgt
      961 tttgccgatc gtaatagcca ccagatctac cttgaaccgg aaggcttaaa tacgattgag
     1021 gtttacccga acgggatctc caccagcttg ccgtttgatg tgcaaatggg gatcgtcaac
     1081 tcaatgaaag gcttggaaaa cacccgcatc atcaaaccgg gctatgcgat tgagtacgac
     1141 tacttcgacc cgcgtgattt aaaaccaacc cttgaaacca aagcgattga cggtttattc
     1201 tttgccggac aaattaacgg cacgaccggt tacgaagagg caggtgcgca aggtttgctg
     1261 gcagggatta acgcagcgtt acaggttcaa ggtaaagagg cgtggtatcc aacccgtgat
     1321 caagcctaca ccggcgtgtt ggtggacgac ctctgtacat taggcaccaa agagccatac
     1381 cgtgtgttta cctctcgtgc cgaataccgt ctgttgctgc gtgaagacaa tgcggacatt
     1441 cgtttaacac caaaagccca cgaactagga ttaattgacg aggcacgttg ggtgcgtttc
     1501 aaccaaaaaa tggaagcgat tgagcaagaa agagctcgtt tgaaatccac ttgggcacat
     1561 ttgcaaatgc caaatctgga cgaattgaac gcgttattgg ctaatccttt agcccgtgaa
     1621 gcaagcggtg aagacttaat ccgccgtcct gaaatgagtt acgaaaagct gacccaaatc
     1681 gccctatttg cccctgctat tgaagacaag caagtggcag aacaggtgga aattgcgatc
     1741 aaatatcagg gctatattga gcatcaatac aacgaaatcg aacgccataa acgccacgaa
     1801 aacaccctga ttccggtaga gtttgactac gacaaagtcg aaagcctctc caacgaagtg
     1861 cgagctaaat tgatgcaaca ccgccctgtc tctatcgggc aggcaagccg catttccggc
     1921 atcacaccgg cagcgatttc cattctgttg gtgaatttga agaagcaagg aatgttgaaa
     1981 cggggtgagc tgtaaatact acaagcggtt ggttttgcag aaatttttgc aaaattgacc
     2041 gcttgtattc tttggagaag gaaatacccg aactaccgcc gtatttttaa tgatatatct
     2101 gcgtaaactc ggtttttcgc cgttcgatca ccaaatgttt gcccaacctt cgtggtattt
     2161 ctgcaatgca cttgttcata ccaattatga aaatctgcga caaggtgtat ttgtgactaa
     2221 agcgtattta gtgatgtttc ttgaaaattt attgtttgaa aaaagtcatt cgttaagtaa
     2281 tcgggcattg cgtattaatg aaaataatga ttaaggaatt tgcaaaatgt tagctaaatt
     2341 aaaccgcttg cttgctcaag ccgaaataaa tttgaccgat caacaaaaag agcagttagt
     2401 tggttttgtg agattattgg ataaatggaa taaggcgtat aacctcactt ccgttcgcaa
     2461 ccctgatgaa atgttggtta aacatatttt ggacagcctt gttgttagtc gtcatttaca
     2521 aggcgaaacg tttattgatg tcggcacggg gccggggcta ccgggcattc cgctggcgat
     2581 tgctaaccct gataaacagt tcgtgctact agacagctta ggcaaacgca tcacttttat
     2641 caaaaatgcg ttgcgtgagc ttggtattaa aaacgttacc ccagtgcaaa gtcgagtgga
     2701 ggaatacact gaaagccagt ttgacggcgt attaagccga gcctttgcct cgcttaatga
     2761 tatgatcgac tggtgttatc atttaccgac tgaaaacggt aaattttatg cattaaaagg
     2821 cgtgtatgat gagcaggaaa taattgaaat caaacaaccg attcagctag tggaagtgat
     2881 taagcttgaa gtacctgagt tggtaggaga acggcattta gtagtgttga aaaagcccta
     2941 atacattgga agtttaatga tacaagcggt cgaatttgta aatttttttg caaatctgcc
     3001 tgcttgtgtt aactattttt cataaagcat acctggttaa taaggtgtgc tttttttata
     3061 acctattatt tcatctgatt tatttctatt ttgtgatgtg cttcacaaaa acaaaaaaac
     3121 ataatgtagt aaaaaatatt cgtaattgaa gcccttattt ctttacctga aaataagcga
     3181 acttaattat ataacgagga ggttcgtaat gaacagggta acaaataagg cagatgagtt
     3241 tgctaaactc agtacgtttg aacgtattgc ttatggtagt ggcgacttag cccaaaattt
     3301 agtttttggt acagttggtg gtttcttact cacttactta actactgtaa acggcatttc
     3361 tgccgtagca ggagcaacga ttttcttaat tgtaaaatgg ggaaatgtgt tttgggatcc
     3421 gtttgtcggt gcctttattg acaaaagcaa gccctcagca cagggtaaat accgcccgta
     3481 tttactgaaa tttgggattc ctcttgtggt tttagcttca ctactatttc ttcctcttga
     3541 atcagtacgt ggctcagtga tttatgcttt cctttcttat tttgccactg cgatggttta
     3601 ttcttttgtt aatattcctt atggctcgct ttctgcttct cttactcgag acacagacga
     3661 aattgctaaa ttaaccacaa cccgtatgac aatggcaaat atcgctaatt tgtttgttta
     3721 taccttattc ccactttttg tgcagttact tgcaccacat gccgaattaa aaaacaccgg
     3781 ctttttcggt attgagctct atttaggtga ttatatgtct gctgctgcag gccctgcttg
     3841 gtttaaggtt tatgcggtct atatggtaat tggatttgtg gcgttaatgc tctcttattt
     3901 tggtactaaa gagcggatta ttccaacagc ggaagaaaca gataaagtca agtattcaga
     3961 tttagttaat gaactcaaac gcaatcgtcc attacagatt ttagggttat tcttcttaat
     4021 aggttttact tttatgttct tcggaaatac ggtgtggcct ttttacctaa aatataatat
     4081 tgggcattct gagtggattg cctccgtcgg tttattaggc tctatccctg ggattttcct
     4141 tgtattctta tggccgaaac tacgtcgcac tatcggtaaa aagaactttt tctatctatt
     4201 tttagggatt ttcgttctag ggcaactttg tctatgggta tggcaatttg actccttcaa
     4261 atactctcca accttaggct atatcggtcg tttcttacaa caatggggat taacttctgc
     4321 taccggtttt atgtgggcgt tagtgcctga ggttgttacc tttggagagt ttaagtcgaa
     4381 aaaacgtgta gcaggtatta ttaatgcgtt aatgggcttg ttctttaaaa tcggcttggc
     4441 gttaggcggt attattccgg gttatattaa tgcggtttat ggttttaacg gctcgctttt
     4501 ggctcaaacc gaagaggctt tatttggtat caatctttca atgatttggg ctccaattat
     4561 tttagcgatt attgcaggat ttgtgatgag tcgctatccg ttaagcgata ctgatattga
     4621 tcatatcaac gaaaaaatta ctgcttattc caaataattt gagtatagga gaatagatta
     4681 tgcaaattca aaatccagtt ttacccggtt tcaatccaga tccatctatt gtgcgtgtgg
     4741 gtgatactta ctacattgca acttctactt ttgaatggtt tccgggagtg agaattcacg
     4801 aatcgaaaga tttagtgcat tggaatttag ttaaaaatgt cttagataca accacgttgt
     4861 tagatatgcg aggcaatccc tcttcaggtg gtatctgggc tcctgatctt tcttatgcgg
     4921 aaggtaaatt ttggttggtt tataccgatg taaaaattac agacggtgca ttcaaagata
     4981 tgactaacta tctaactact gccaaagaaa ttcaaggtcc ttggtcagct ccgattaaat
     5041 tgaatggtat tgggtttgat gcttcgttgt ttcacgatga tgatgggcga aaatatttag
     5101 tgcaacagac ttgggatcac cgtgaatatc atcacccctt tgacggtatt acgttaacgg
     5161 aatttgatgt tgccacaatg aaactcaaac ctgaaaccca gcgtacgatt tttgaaggta
     5221 cagaagtgaa attggtggaa ggtccacata tttataaaat taatggcgaa tattatctgt
     5281 ttgcggcgga aggcggcaca gtatttacgc atcaagaagt agtggctcgt tctaaaacat
     5341 tggaggctca ctctttccaa ctggctccaa atacaccgtt tattactaat tttgatacac
     5401 ctaattctta cctacaaaaa caagggcacg gttcattagt tgatacccca agtggtgaat
     5461 ggtattacgc ttccctcaca gcaagacctt ggaatcatgc aacagaatcc gcaaccgatc
     5521 ctcgtggttg gtcaacatta gggcgtgaag ccgccattca aaaagtagaa tttgatcaag
     5581 acggctggcc ttatattgtg ggtggacacg gtggaaaaac ctttgtagaa gccccaaaag
     5641 atgcaatttt taccgaagct cctattgatc attctcaaca cgatgagttt gatagctcaa
     5701 cgttagatat taactggaac acttaagagt gccttttagt aaagagatgg gcgaagttgg
     5761 taatggtgtt ttaaaattga ttgggcgagg ctctcttgca aatacacacg atttatcgct
     5821 aatcgcacgc cgttggcaag ccttctattt tgatgcggaa accaaagtga aatttacacc
     5881 gttttcttat caatctatgg caggtttaac taactattac aatcactcac actggtcttg
     5941 gattttcatc accaaaaatg atcaaggaca acaggtgatt gaagttgcag aaaataaagg
     6001 tggactcaga aatgggcaat atacttctta ccttaaagat aaagcaattg tcattcctga
     6061 tggaacggag tatgtttggt ttaagactaa agttcggaaa gaaacttacc gttatgaata
     6121 ttcattcgat ggcaaacaat ggcacactat tccggttgaa ttagatgcgg cagtgctttc
     6181 agatgattat gtgttacgca gttatggcgg tttcttcaca ggagcttttg tcgggctggc
     6241 tgcagtggat tactccagtt atgaatctac agcagaattt agctactttg actataaaga
     6301 gttaggcgat aagcaactta cagacggctc attctcttgg gaaaaatctg aaagttgctt
     6361 tggctaaaat gaggagaata tcacaatgaa acaacgcaga tttggtaaaa ccaacaaaat
     6421 ggtgtcagaa attgggttag gcacttggca gcttgggacg aaatggggtg aaccgttcaa
     6481 tcatcaagag gcgatggcaa ttttagaaac cgcttatgag caaggcatca attttattga
     6541 taccgctgat gtgtataaca acgggcaaag cgagaaagcc attggtgata ttttgaaaaa
     6601 ataccctgac tttttctatg tcgttaccaa atgtggacgt gcgttaaacc cgcatactgc
     6661 tgaaatgtac acgccacagg caatcgaaaa atttgttgat ggtagtttaa gccatcttgg
     6721 cacggaaaag ttggatatga ttttgctcca ctgcccgcca acgttggttt atcgcaatga
     6781 tgagattttt acgaaattgg aacagcttaa agccggcgga aaattaatag actacggtgt
     6841 gagtatcgaa acggcagagg aaggcttgtt ggcaatggag tatgacattg cggcaatgga
     6901 agttatcttc aatatgttcc gcttaaaacc attggagcaa ctcttcccaa ccgctcagca
     6961 aaaaaatgtg ggtattatcg cccgtgtact gttggcaagt ggtttgctta caggcagata
     7021 taatgcctgc acccaatttg gcaaagacga ccaccgcaca tttaaccgca atggtgaagc
     7081 cttcgataaa ggcgaaacct tttcgggtgt ggattatcaa ctcggtttac aagcggtgga
     7141 agaattgaaa gccctattcg gcacggaaga tttaattcct tacgccctac gttgggtatt
     7201 gatgaatgaa gctgtgagta cagtaatccc gggagcaagt aaggtttcac aagtaattac
     7261 taacgcagcg gtggataatt tccctccact cactgaaaat gaaatgcaaa aagtggaaga
     7321 tatttacaac cgcttaattc gcccaagtgt gcatcatttg tggtaattta tccaacgcta
     7381 taaaaaatgg aactctaact tgagttccat ttttgtcaat tttagcctta aacctctaac
     7441 tgtttagagg ttttcttaaa cagagtcaat gcaataaacg caaaggctaa gaaacaggaa
     7501 aggatcaggg catagaaacc accgtcccag ccgaacatat caaccagttt acccatcaca
     7561 taacctgcac ttgcagagcc taacaagtag ccgaataaac cggttaaacc tgttgctgta
     7621 ccggtggcaa cacgaggcac taaatccgct gcttgtaaac cgatcatcat caccggcccg
     7681 tagattaaga aaccaatcgc cactaagcag atgttgtcga ttaacgggtt gccggctggg
     7741 tttttccagt acaccacaat cgcaattaac acaccagcaa ggaataacaa catcggcggc
     7801 gcacggtgac ctttgaatac tttgtcgctt aaataaccgc ttgcaagcat accgaagata
     7861 ccggcatatt catacaagaa gtacgcccag ctctgtttat ccacagagaa atgtttcact
     7921 tcttttaagt aagtcggtgc ccagtcgata atgccgtagc gaatgaaata gacaaacacg
     7981 ttggcaatcg caattgccca caggaatttg ttgtttaaaa tatatttgta gaaaatgtct
     8041 ttggaagaga gtgtgtgggc agactccact ttttccacca ctttctcgcc tttgtactca
     8101 tcaatcggcg gtaagccttg agattccggc gtatcacgca tcaaatagaa catcacaaac
     8161 gccagtacaa tcgcaatcaa cgccggcaga tagaacaggg attgccacac gccaaaaata
     8221 gataagccta aaatcgccaa cggcccgatt aaaccgccgc ccaagttgtg cgacacgttc
     8281 caccagctcc accacacgcc acgttcagag gttgagaacc agttggtcat cgttttcgcc
     8341 cccggcggat agcccattcc ttggaaccag ccgtttaacg ccgccaaaat gatcataatt
     8401 ggaatagacg aaagcacgcc cggtactaaa ccgaacacca gactcacaat cgctgaaccg
     8461 agcaagccga tagtgataaa gtatttcggg ttagaacggt cggaaacgtt acccataata
     8521 aatttactga agccgtaagc gagtgataat gccacgccca ccgtacctaa gtctgctttg
     8581 gtgaagccat attcatcaat caaatacggc attgccaacg agaagttttt acgaattaaa
     8641 taatatgccg catagccgat aaagacgcct gcaaacactt gccaacgtaa tttgcgatat
     8701 tccgcatcaa ctctgctggc ttccactttt tgttctgctg gtggagaggc ttttagaaag
     8761 gaaaacattt tactactcct ttaagaatga aatcctatgg ctactataat ctttcatttg
     8821 gagcagaaaa agtggatttt tgaaaatttg tgagcaagat taaaattatt ttgagcgaaa
     8881 acgaaaagcg gttgaatttc cttctttttt tgcaaaaaat ttgagaaatc taaccgcttg
     8941 tttcacttaa aaatcagcta tttctgcttt ttcaaaaatt ccacgcccgt atcagggaag
     9001 tcagtaaaga cgcctgttgc acccgcttta ttcagcaggg catcgtacat ttcatcgaca
     9061 ttttggaaaa agtccggcag agcatctttg cgcacggtgt atgggtgaag ttccattttg
     9121 tacttcgcca actctttcac taatggcgtg taaacaatgt tgccgacttt agatttttct
     9181 ttatccacca acatatacca gcccgggccg acaccatcgg catattttgc cacttctgcc
     9241 atcgcaccgt ctttgaacat ccagtcgtag tcgtaattaa cccagttgcc gtttttatct
     9301 ttctcttcgg tttcgtgcca atcggtgtag gcaactaact gcaccaattt tacgtccata
     9361 cccagtttcg gtagtaattc ggttttgatc cgtttcagct cgttgaaatc gaaggtttgc
     9421 aggtaaattg gtgaggtttt gctgtcgtag ccatattttt tcaacacttt cagggtttcg
     9481 agtgcaatgt ctttaccctc tttatggtgc aaccaagggg ctttaatttc aggatagatc
     9541 ccaatttttt tgccggttga tttctctaat ccttgaataa attccagctc ttcttccaaa
     9601 gtgtgaatgg tgaaatggga ctgccacatc gggaagcggt tcgggtaaac ttgtgcctgc
     9661 ttgccgtctt tcacctcaaa attctcggtc atttcaaggg ttttaatctc atcaagggtg
     9721 aaatccacca cataataacg accgtctgct cgtttacgct cagggaattt tttcgccaca
     9781 tcagttaaac cgtctaaaaa gtggtcgtga atcacaatca ggcggttatc tttggtcata
     9841 gcaagatctt gctctaaata gtccgcttgt tgcccgaatg ctagagcttt agattcaagc
     9901 gtatgctcag gtaaataacc gcttgcacca cggtgggcaa tcactaattt atcagattgt
     9961 tgtgcatagg cacccaatgc tgtcgcggac aatgctaatg cagcaaccaa tgttttgaat
    10021 ttaaatttca tagaattacc tcatataatg taaaaataaa aggaagaaca aaaatgtcct
    10081 tccttttgat aagttattta ttatttacca tagtgatctt ctaattttgc tttgtgcttg
    10141 ccttcttcaa ccatcacaat gaaggttaag agaatggcta atacaccacc accgatcatc
    10201 acgtagaaac cgccgtccca gccaaattta tctgctgccc agccaactac tgcagaggct
    10261 gccactgtac cacctaagta accgaataaa ccggtgaaac ctgctgctgt acctgctgct
    10321 tttttcggtg caagttcaag ggcgtgtaag ccaatcaaca ttaccgggcc gtagattaag
    10381 aaaccgatag tggtcatcca aatgaagtcc attaattgat atgggttttc ataccaagca
    10441 tggcctgcat attgtgcaat ttcagattcc ggtgtcgcag ggttgagcca gaacgcaacc
    10501 actgctgcgg tggtaagaat cataaagatg aaaccggtta aaccacgttt gcctttgaac
    10561 agtttgtcag agacccaacc acagagtaat gtacctggaa tcgcagctaa ttcataaatg
    10621 gtatatgccc atgctgtacc tttaatgttg aagtgtttta cttcgcctaa gtacaccggc
    10681 gaccatttca atacgccata gcggattaag taaacgaaga cattcgcaat ggcaatgtac
    10741 cataacagtt tgtttttcag cacataagtc acgaaaatct ctttggtgct taaatcgtgt
    10801 tcataggttt tttcgttata gtcgtcaggg tagtcgttac gccatttttc aactggcggt
    10861 aaaccgcaag attgtggagt atctttcatc acaaggtaga ttggaatagc tgcgatcatt
    10921 gctgcaattc ccgggaaata aagggcttgt tgccatacat cttttgccgt tgcttctaca
    10981 ccatactcac tgaaatagag cgcacttgct aatagcacca tcgcccccgg caccataccg
    11041 cctaagttgt gagcggtatt ccagatagaa acgatagtac cacgctcaga tttagaccac
    11101 cagtgaacca ttgttcgtcc acacggtggc caacccatac cttgaaacca gccgtttaag
    11161 aagatcatta cccacattac taaaatgctt gatgttgccc aaggcattaa gcccataagg
    11221 gtcatacata gacctgaaag taacaatccg gtcggtaaaa atactttcgg gttagagcgg
    11281 tcggacattc ctgccatcac gaattttgat aaaccgtagg ccaggccggc agctgtacca
    11341 atcacaccga gatcggtttt tgtatacata ccagcttcaa tcaagccttt ttgtgctaag
    11401 tcgaagtttg cacgcacaaa atagtaagcg gcatagccga agaaaattcc tgcaaacact
    11461 tgccaacgca agaatttata tcttgcatcg actttgtccg ccggtaattc cgcaatgtgc
    11521 ggagcgggtt taaatggacc aaacatagta ttctccaata ttacttagta aaac
//
LOCUS       DANLXQ010000078        11369 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.078, whole genome shotgun sequence.
ACCESSION   DANLXQ010000078 DANLXQ010000000
VERSION     DANLXQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11369
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.078"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            14..175
                     /locus_tag="PXD94_001559"
     CDS             14..175
                     /locus_tag="PXD94_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286410.1"
                     /translation="MEMSIEKIAELLSDKEKDDYPFSIEERQFAAQASADTWEAILNN
                     TIQPFDFKK"
     gene            183..545
                     /locus_tag="PXD94_001560"
     CDS             183..545
                     /locus_tag="PXD94_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286411.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESETKVILLGTKFNNKRKTFNFANTKTQQKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            558..1475
                     /gene="nagK"
                     /locus_tag="PXD94_001561"
     CDS             558..1475
                     /gene="nagK"
                     /locus_tag="PXD94_001561"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL6286412.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYECGCGNQACLDTYISGRGFELLFNDLVGEKISAKAIIERF
                     YQQETKVVAFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(1546..3495)
                     /locus_tag="PXD94_001562"
     CDS             complement(1546..3495)
                     /locus_tag="PXD94_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL6286413.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANETFNQLHKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDIDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKE
                     ALAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(3528..4316)
                     /locus_tag="PXD94_001563"
     CDS             complement(3528..4316)
                     /locus_tag="PXD94_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL6286414.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            4441..4671
                     /gene="xseB"
                     /locus_tag="PXD94_001564"
     CDS             4441..4671
                     /gene="xseB"
                     /locus_tag="PXD94_001564"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL6286415.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEEALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            4677..5570
                     /gene="ispA"
                     /locus_tag="PXD94_001565"
     CDS             4677..5570
                     /gene="ispA"
                     /locus_tag="PXD94_001565"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL6286416.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVVQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEYSQNVAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGETDKIGKIVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(5605..6405)
                     /locus_tag="PXD94_001566"
     CDS             complement(5605..6405)
                     /locus_tag="PXD94_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL6286417.1"
                     /translation="MCQLLGMNCNTPTDITFSFDGFRRRGGLTDHHTDGFGIAFFEGK
                     GVRVFRDNRPGASSPMAEAISQYQIKSLNVIAHIRKATRGDVNLENTHPFIRELWGEN
                     WVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQ
                     ITSRIAEHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKIT
                     DKVTIIATQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAQPHV"
     gene            complement(6530..7315)
                     /gene="znuB"
                     /locus_tag="PXD94_001567"
     CDS             complement(6530..7315)
                     /gene="znuB"
                     /locus_tag="PXD94_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL6286418.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     FFSIIAVSGGLLLSSWKDTPAGPSVVICAGLIFLLSLFKKEYH"
     gene            complement(7402..8739)
                     /locus_tag="PXD94_001568"
     CDS             complement(7402..8739)
                     /locus_tag="PXD94_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL6286419.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            complement(8749..9237)
                     /gene="mreD"
                     /locus_tag="PXD94_001569"
     CDS             complement(8749..9237)
                     /gene="mreD"
                     /locus_tag="PXD94_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL6286420.1"
                     /translation="MRTNPIFQILVIIAIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            complement(9237..10292)
                     /gene="mreC"
                     /locus_tag="PXD94_001570"
     CDS             complement(9237..10292)
                     /gene="mreC"
                     /locus_tag="PXD94_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL6286421.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            complement(10394..>11369)
                     /locus_tag="PXD94_001571"
     CDS             complement(10394..>11369)
                     /locus_tag="PXD94_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL6286422.1"
                     /translation="KGQGIVLDEPSVVAVRQDRMGSLKSIAAVGSHAKLMLGRTPKSI
                     MAIRPMKDGVIADFFVTEKMLQHFIKQVHSNNFMRPSPRVLVCVPAGATQVERRAIKE
                     SAIGAGAREVYLIEEPMAAAIGAGLPVHEAMGSMVIDIGGGTTEVAVLSLNGIVYSTS
                     VRIGGDKLDEAIIAYVRRHFGSAIGEATAERIKQEIASAKIESDDDVKTMEVHGHNLA
                     EGAPRTFELNSRHVLEAIEQPLNGIVEAVKSVLEQCPPELAADIFERGMVLTGGGALL
                     HNLDRLLSDSTGVQVVVAEDPLTCVARGGGKALEMIDMHGGDVFSIDD"
BASE COUNT         3524 a         2525 c         2151 g         3169 t
ORIGIN      
        1 taaggagctt agaatggaaa tgtcgataga aaaaatagct gaactattaa gcgataaaga
       61 aaaagatgac tatccgttta gtattgagga aagacaattt gccgcccagg ctagtgctga
      121 tacttgggag gcaatcttaa ataacactat tcaacctttt gacttcaaaa aataggcttg
      181 ctatgtatca agtttttatt gcagaaccaa aagacccaaa cttagagaaa ggcttattga
      241 aacacttcat taagtcttat cttactcaag aagaactcga tttcctgcat aaaattctca
      301 ctaatttacc caacatcaga cctttttggg gcgataccgg ttacaaatat atcgtcattg
      361 ataatgttta cttcttgtat gaagtttccg aaagtgaaac gaaagtcatt ctacttggca
      421 ccaaatttaa taataaacgg aaaactttta attttgcgaa tacgaaaacc cagcaaaaat
      481 caatgaagaa atggattaat gatttagcaa tttataaacc gcataaaatc aatcaatcgc
      541 aataagagag cgtaattatg gcaatttatt atggcttaga tgtgggcggc acaaaaattg
      601 aattagccgc ttttaatgaa aaactagaaa aactacacag cgaacgtgtg ccgacaccgc
      661 agacaaatta tcaagattgg ctgacggcaa ttaaaacgct ggttgagact gccgatacaa
      721 aatttgacga aaaaggcact gtcgggctgg gcattccggg ctttgtaaac cgtgaaaccg
      781 gcattgcgga aattaccaat attcgagtgg ctgacggcaa aacgattctg aaagatttag
      841 ccgaatgttt agggcgtgaa gtgcgtgccg aaaacgatgc taactgcttt gccctgtctg
      901 aagcgtggga cgaaagtaat aagccttatt ccaccgtatt agggttgatt atcggcaccg
      961 gttttggcgg tggttttgtg tttgacggta aaattcattc aggcagaatc ggaatggcag
     1021 gcgaagttgg gcatatgcaa cttaactacc acgcacttaa attgctcgac tgggataaag
     1081 ccccaattta tgaatgtggt tgtggcaacc aagcctgttt agacacctat atttcagggc
     1141 ggggttttga gttactgttt aatgatttag taggagaaaa aatctcggcg aaagcgatta
     1201 tcgaacgctt ctaccaacaa gaaaccaaag tggtggcatt tgtcgaaaaa tatattgagc
     1261 taatggcaat cagtatcagc aatctgatta cgattttaga tccggatatg attgtattcg
     1321 gtggcggttt atccaacttt gattatatct accaagctct accaaaagca ttaccaaaac
     1381 acttaatgcg ttcagcgaaa gtgccggtca tcaaaaaagc aattcacggc gactcaggtg
     1441 gcactcgtgg tgcagcagca ttattcttaa agtaatgaac aaatttgaga gagggcaagc
     1501 ggtcattttt aataaaaata ttgcagccct ctcttttaat ttgccttact gcttagtttc
     1561 ttccggtaat ttatataact gatttttata taaataactg ccaagcaatg cgtgtgctaa
     1621 cgcctctttt ttaaacgatt ccggctgttc tttaggggtg agtttatagt tagctttatc
     1681 gtagctaaat ccttctatcg gtaaatttgg tcgcattacc accacatcat ttccccgcat
     1741 catcgcctgt gtcgcatcat attgcataaa ggcacggttt gggtcaatgt cttgcgttaa
     1801 atcataccct atcatcggat attgaccact tattcccgcc acagaaagta atgttgtcgg
     1861 catatcgagc tgactcacta aacggttatc cgttcttggc tgaatgtgtt cgcctaataa
     1921 aagtgccgga atatggaagt gttgaattgg cactaaatgc tcgccaacgg ctcttgaatc
     1981 gtgatctgca atcactaaaa acaccgtatc ttgccagtac tctgattttt tcgctaactc
     2041 aaagaaatgc ccgatagcat aatcggcata ttttgccgca ttatggcgag tctgcttcgg
     2101 ttgttcatag agttcaattt tgccgtccgg aaattcaaac ggatcgtggt tactggaact
     2161 gaataccaaa ctaaaaaacg gtttacctga tttgtgcagt tgattaaaag tttcatttgc
     2221 cttatcaaac agatcttcat cactcacgcc ccaagttgcg gtaaatttag ggtttttata
     2281 atctttttca tcgataattt gctggaaacc attaccgtag aaaaagctcg ccatattatc
     2341 aaaatgcttc tccccaccat aaataaagga agtatggtat ccttgacgtt ttaataattc
     2401 ggcaatacta aaaaagttag tttggctttt cgtcagtttt accacagatc ttgccggtgt
     2461 tggtgtaaaa ccagccgtta ccgcttcaat tcctctcacc gaacgagtac ctgttgcata
     2521 aagattggta aaataccagc cttgttctga aagtttatct aaatttggag tgagattttt
     2581 ccctcctagc gaaccgacaa attgagcccc taaactttct tctaagataa tcacaatgtt
     2641 tttaggcttt ccttgataag tcgctttatt ttgcgtgagg gtgggaattt catctgaaat
     2701 ataatctgaa gctggtcgat tacgaacctg cttaacaatg ttgaacattt cctccacaga
     2761 catttttcca tactgttcgg aagatttatc ttcatctttc aattgctgaa ccgcataaag
     2821 cacggaataa cctgaattta gcaccagtga attgacaagc ggatcgcttg aaaatgccac
     2881 cattgccgga ttaatccctc gatgggcaaa actggaacgc ccaccaacaa aaactattgc
     2941 agcaaccaat aaagccagta gcggacgcat tttccagctc gggaaatgca aatttttggt
     3001 tgcccaaccc gataatttcc aataaatgat gagagaaatc atcgcaatgc caatcccaca
     3061 aataacagcc cataaatgtc cgttaatcag cattgtgaac acttctctcg gatggaccaa
     3121 atactcaatg aaaagacgat ttgggcgaag gtcaaaggtt tcaataaaat tcggtgtggc
     3181 gacttccata aaaagaataa atacactacc gacagttagc caaattctta aaacagtctg
     3241 ccaaatcgaa cccagtacat ttttatgaaa acacaacacg ctaaataatg ccggtaagcc
     3301 aaatagccaa catagcgaag atatatcaat gcgaatacct tgtagtatta atggtatcca
     3361 tccatcaaca gcggatactc gatcagattg ccaaacgcct aacactaaac gggataaggt
     3421 gaggataact aaattaatcc caacaaatag taggattgga aatagcacag ttcttgattg
     3481 actgcgttgg tacattttga atctcctttt aaaaaaatat cggcatatta taattgaata
     3541 atgacaggat cgcctacttt tatcacacca ctgttttgtg gcacaaggtg aataccgaac
     3601 accggcttac ctttttcatc gcaatgcttt tcttttaagg ttctaaacgg ctccgatttg
     3661 gggtctaact caagcgtgtt taaatcccgt gtaatcaaaa tgcaacgagt acataatgcc
     3721 gctttggtaa atagcacttc gccaatttga acttgttgcc actgctcttc ttcaaatgcc
     3781 acattgccat caatcacaat attcgggcga aaacgagcca tttcgagtgg aatcggcgac
     3841 cattgttgca cttgctcaag cgattttaga gacatcaaac tcaccgggtt gctatctgca
     3901 aaactcattg ggtgatcggc aaaattcgca acaattcgtt ggctttgcga gccaagccaa
     3961 cgtagttgca cagccctacc aaaaatttca ctcagccatt gattgaccgc ttctgccgcc
     4021 actttggcat taaaatgtgt tccccacact tcactactct gctcttccac aaaatcttgg
     4081 taaagtgcaa cgcatttttg accgcttgta tgggtaatca ttacccctgt cgcaatcggg
     4141 aaagccgcta gacggtataa ttccggatct ttacgggcag taataaattt gccgttaggc
     4201 tcggctagca taaattcacg gtcaaaattt aagcctttcg gttcaacaaa tgcctgtgaa
     4261 acttgaatag cttgagttga ttttatcggg taaatattga gctcggtaac ttgcatagaa
     4321 aatcctccat ttggaaatag gataacaata cctgcaagcg gtcgaaaagt aaaaggaatt
     4381 tgcaaatccc ttgtgagaac ggtaaacttg agcaactttt acagcaatag gtcagcaaca
     4441 atggcaaaga aacccactca agattttgaa agcacactta aagaattaga agcaatcgtt
     4501 acccatttgg aatccggtga tttaccgctt gaagaagccc tcaacgaatt tgaaactgca
     4561 atcaaattag tgcaacaagg gcaagaacgg ttacaaaaag ccgagcaacg tattcaaatc
     4621 ttattaaata aaaacgatac tgccccactt agcgattacg aaagagaata atcacaatga
     4681 gctatctatt agcaaccgat ctgactcacc tgcaacaacg gattaaccat tttctcgctg
     4741 aaaaattaaa aacaacggat agcgttggtt cgcctttagt agaagcaatg gcttatgcgg
     4801 tattgctagg cggtaaacgg gtgcgaccgt ttttaatcta tgccaccggc agaatgcttg
     4861 gtgtgccgtt ggaaaaatta gatcatagtg cggcggcaat ggaggctatt cacgcttact
     4921 cgctggttca cgatgattta ccggcaatgg acgatgatag attacgccgt ggcaagccaa
     4981 cctgccatat tgcctttgat gaagctaccg ctattttagc cggtgatgcg ttacaaagtt
     5041 ttgcgtttga actgctggca accgatccgc atttgaccga ccgagaaaaa gttgttcaag
     5101 ttacagaatt ggcaaccgct tccggtgcaa aaggaatgtg cttagggcaa agtttggatt
     5161 tgattgcgga aaataaagcg gtgagcttgg aagagcttga attaattcat cgcaacaaaa
     5221 ccggggcatt gattctatca tccgtgatga tgggattaaa cctttccgaa tattcgcaaa
     5281 atgtagccat aaaacaaccg cttgtaaact atgccaaagc gattggctta gcctttcagg
     5341 tgcaagatga tattttagat gtcattggcg aaacagataa aatcggcaaa atagtgggtt
     5401 cagatgaaca cttaaataaa agcacatacc cgaaattgtt gggcttagac ggggcaaaac
     5461 aaaaagccga aatgctttat caaaccgctc tccattcgct ggaacagttg cctttcgata
     5521 ccaccgcatt aaaagagtta gctaatttta ttgttaagcg tgaaaactaa gaaaaaacct
     5581 agccgaagct aggtttttta gtgattaaac gtgaggctga gcttccggca atgtgccttc
     5641 aatttcatat accacatcgc cgtctttaaa gaaaacatag ccaccgtttt tcattttggt
     5701 ccagttttca tttttggtaa gtggctgagt ggcaataatc gtcactttat cggtaatttt
     5761 ggtatattga ctaaaatcaa tcgacacatc atcatcacgc aaggctttgc caaacggtgc
     5821 tttacggcaa acgtaatgca gattagttgt acaacgggca atcatccaat gaccgttgga
     5881 taaaacaaaa ttaaataccc cgtgttccgc aattcttgag gtgatttgca ccacggcatc
     5941 aaaaatctct ttttcgcttg gtttagccgg atacatctct tttaattccg acacaatacg
     6001 gcaaaatgcc gcttcggaat cggttgtacc aatcggctgg tagtggcact cctcgcatac
     6061 cccaacacct tccactgtgc cgttatgggc aaacacccag ttctcgcccc ataattcacg
     6121 aatgaagggg tgggtatttt ccaaattcac atcaccacgc gtagctttac gaatatgtgc
     6181 aataacattg agcgatttaa tttgatattg gctgattgct tctgccatcg gcgatgatgc
     6241 tcccgggcgg ttatcacgaa aaacccgaac ccctttgcct tcaaaaaagg caattccaaa
     6301 cccatcagta tggtgatccg tcagcccacc ccgacggcga aatccatcaa aagaaaaagt
     6361 aatatcggtt ggcgtattac aattcattcc aagtaattga cacataattt gcttcttaaa
     6421 accaaataat ttacaacaaa aaataattgt tacatttaaa cagattgatt agaaaattca
     6481 atgctcttta cataaaaatc ttatgcttta ttttcataca ttatgctgtt taatggtatt
     6541 cttttttgaa tagcgataat agaaaaatca agccggcaca aatgactacc gaaggccctg
     6601 ctggtgtatc tttccaactg gataacaata aaccgccact gactgcaata atactgaaga
     6661 aaatcgcaat caacaccatt ttttcaggcg ttttagcaaa gcgtcttgcc gtagcagacg
     6721 gaataatcag cagtgaggta ataattaaag caccgacaaa tttcatactt aatgcgatag
     6781 ttaatgcggt taaaatcatc agcaataaac gtaagcgagc cacatttaat ccttcaattt
     6841 gagcaagttc cgggctgatg gtaatcgaca acaaccgctt ccaaaacagc attaaaatca
     6901 aacaaattag ggttgctccg attccgataa acaccacatc agaaaaatta attgccagta
     6961 aatcgccgaa taagtaagac atcaaatcca ctcgtacatt atccaacaaa ctaatggtaa
     7021 ttaccccgag agaaaggcta gtatgggcga taatccccaa cacggtatcc accgaataat
     7081 tggttttttg ctccaaccaa accagcaata atgcaagaag aatagtcatt ccaattacag
     7141 caagatacgg atcgagttct aaaaaaatcc ctaatgccac gccgagcaat gcagagtgcg
     7201 aaagtgtgtc gccgaaatac gccatttttc gccacaccac aaaagcccct agaggtgcgg
     7261 taataaaggc taacaaaaca ccggcaagcc aagccggaaa taaaatatca aacatattgg
     7321 ttctcaaagg caagtaaaac aagcggttat tttttcggaa aaatttgcaa attttttgct
     7381 gatttagacc gcttgtcata tttatttata acgttgaatc gggctggctt gatcctgtgc
     7441 tttcagccac gccatttttt ctttaatctg tttttccatt ccccgctcac ttgggaagta
     7501 gaattcggta tctttcagct caggcgggaa ataattctcc ccggcggcat aagcgtttgg
     7561 ctcattgtgg gcatagcgat actcttcgcc atagcctaaa gatttcatta attgagtggg
     7621 agcattacgc aaatgcgccg gcacatcgta gtcttttgcc tctttagcga ggcgctttgc
     7681 ttcattaaaa gcttgataga ccgcattgct ctttggggca actgccaaat aaatgatcgc
     7741 ttgggcaatc gctctttcac cttcataagc ccccactcgg gtaaagcaat cccaagccac
     7801 aatcgcaatt tgcattgctc gagggtcggc attaccaata tcttcagaag caatcgcaag
     7861 cagccttctt gccacataaa gcggatcacc accggcggtt aaaattcggg cataccagta
     7921 taatgctcca tcagccgatg agcctcgaat ggatttatgt aacgcagaaa tgagatcata
     7981 gtaacgatca ccgcctttat caaaacgagc ttgtcgttcg cctaacactt cggtcagcaa
     8041 acttttattc agcactttcc ctttagctga atcttgtgcc atatctacca ttaattccaa
     8101 acagttcaag gcaaaacggg catcaccatt aacataatca gcaagcaatg ccagtacatc
     8161 atcttcaagt accaaagttt cattgcctaa accacgttcg gtatcggcta tcgccatttg
     8221 caaaattttt agaatttcga ccgcttgtaa cggctttaaa atataaattc tggctcggga
     8281 aagcagtgcg ttatttagct caaaagaggg attttcggta gtcgcaccaa taaaaataat
     8341 agtgccgtcc tcaatatgcg gtaaaaacgc atcttgctgg cttttgttaa agcggtgaac
     8401 ttcatccaca aaaagcaacg ttcgtctgcc ggcttggcgg tttaatttgg cttgttcaat
     8461 cacctctcgg atctctttta caccgctggt cacagcggaa atacgttcaa cttccgcatc
     8521 aagatgatgg gcgataattt cagctagggt cgtttttccg gtgccgggcg gtccccaaaa
     8581 aatcatagaa tgactatgtc cggcttcaat cgctctgcgt aacggtttgc cttcgccaat
     8641 taaatgagat tgcccgacat attcagccaa gtttcgaggt cgcattttgg ctgcaagcgg
     8701 tcgaaaatct tcactaaaat caaaagacaa actactcatt ttaacctctt aacgtaaccg
     8761 cagttggcga cgaatatgac gcattaataa gaaaatccaa gaccaaagca caccactgat
     8821 tacagcacct aacaattcct gtgaattaaa ttcggcactg tgtaaaatgt attcaatcac
     8881 gaaaataaag aaacggatca gcacaatata tgccattact aataggcttt gtagccatag
     8941 cgataagttt cgcagaatca gatggtattt cgcaacaaaa taaatcgcca ccgataacac
     9001 taaggcgtgg atgcctaaaa tcgagccaag aatcagatcc cagacgatgc cggcaataaa
     9061 agccgtcccc acactcattt tatcaggtaa tgccaatgcc caataaatta acaccaaaac
     9121 gacccaagta gggcgtagcc cttggaatcc taccggccaa ggcataatct caagtacaaa
     9181 agcaaccaca aaaatcgcaa ttatcacaag gatctgaaaa atcgggtttg ttctcattat
     9241 tccgctcctt cttccgtatg atagttttgt tcattttccg cttctggttg tgctgccgga
     9301 ttctcattta tctcatcatt ctctatttgt tcttcttttg cctctagctg cacttcagtt
     9361 ttcttgttag acatctgttt taaacgttca aatggattca cgctattacg tctttcttca
     9421 acaacttcac gtacttgcgc tggcgacaag ctttgggagt agtttttctc ttccgccgtc
     9481 ggccaaagca gtaataagta acgtaagcga tcaaatgagg ctaacggacg agcaacaatt
     9541 ctggcaaatt gacttttggt atcattttca accgtttcta caatcgccac cggatatccc
     9601 tcagggaaac gtccacctaa gcctgaagtc accagcacat caccttttac cacatcaata
     9661 gaacgaggca agttatcaat gaccaattca ttattcaccc ctgtaccact ggcaatgcca
     9721 cgtacatcat tgcgtagcac ttgaacagga atagcatgag taatatcaga gagcaataaa
     9781 acacggctag atttttcccc tacagaaata acttgtccaa caacacctct ttcatcaata
     9841 ataggttgcc cgacaaaagc accgtctaaa cgcccttgat taatcactac ttgctgacga
     9901 taagcatcca tctcagcagc taatacttca gcaatttttt tatactcatc ttgccgaagc
     9961 ggagaactca ataacaatct taaacgctga ttttcaacct ttagctgatc aagcaataat
    10021 aaatcggcac ttttttctct tagctgctct tttaataaat tattctcaag ctgtaattta
    10081 ttattatcaa taaaattgcc acttacccca tctaaaattg aacggggggt attagcaaaa
    10141 taatataatc cacttaccgc ggtttctaat agattacgcg cttgaatcat taaagagcta
    10201 cgcccatcgg aaataatcag cataatagaa aatatcactg cgataatcag gcgcatccct
    10261 aaagagggag ctttagcaaa aataggtttc attagagaaa tatatgtgaa tttattggga
    10321 atctataacg aaaattgaca atagaatcaa tccattgcca acctccattt aaacagctgc
    10381 taagagaaat gtattaatca tcaatactaa atacatcacc accgtgcata tcgatcattt
    10441 ctaatgcttt accaccacca cgagctacac aagttaatgg atcttctgct actacgactt
    10501 gcacgccagt tgaatcagaa agtaaacgat ctaaattatg taataatgca ccacctcctg
    10561 ttaacaccat accgcgctca aaaatgtctg ctgccaattc aggtgggcat tgctctaaga
    10621 ctgatttaac tgcctctaca ataccattta atggttgttc aattgcttct aatacatgtc
    10681 ttgagttaag ctcgaaagta cgaggagcgc cttcagctaa attgtgtccg tgtacttcca
    10741 ttgttttaac atcatcatcg gattcaatct tagcactagc aatttcttgt ttaattcgct
    10801 ctgccgttgc ttcaccaata gcagaaccaa aatgacgacg tacataagca ataatagctt
    10861 catctaactt atcaccacca atacgcacag aagttgaata cacaatgccg tttaaagaaa
    10921 gcactgccac ttcggttgta ccgccaccaa tgtcaattac cattgacccc attgcttcgt
    10981 gaaccggcaa acctgcaccg attgcagccg ccattggctc ttcaattaaa tagacttcac
    11041 gcgctcctgc tccaattgca gattctttaa ttgcacggcg ttccacttgt gttgcacctg
    11101 ctggcacgca cactaataca cgagggcttg ggcgcataaa gttattacta tgtacttgtt
    11161 taataaagtg ttgcaacatt ttttctgtta caaaaaaatc cgcaatgaca ccatctttca
    11221 ttggacgaat cgccattatg ctttttggtg tacggcctag cattaatttt gcatgtgagc
    11281 ctaccgccgc aatacttttt agcgatccca tacgatcttg acgtacagca accactgagg
    11341 gttcatctag cacaatacct tgaccttta
//
LOCUS       DANLXQ010000079        11220 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.079, whole genome shotgun sequence.
ACCESSION   DANLXQ010000079 DANLXQ010000000
VERSION     DANLXQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.079"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..2782
                     /gene="mscK"
                     /locus_tag="PXD94_001572"
     CDS             <1..2782
                     /gene="mscK"
                     /locus_tag="PXD94_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL6286423.1"
                     /translation="GGSEDLSALYSAQLDEKKQEQQNLQAEIANLQTAINNKIVEESQ
                     NKLDQAQAVQNQQNNATTNPVILRELDINTKVTQELLKQTKDMTQLSQDNLRIKSVLD
                     NLQQTQRNIEEQISSLQGTLVLSRIINKQKQSLPQDEMISGLSKQIADLRVRVFDITE
                     FKDSFADINAYISRIEQDEKTTFTSKEKEQLSKILQERSDTLTEMIKSLNNQLNLLIN
                     IELNQQQVQTISDALQQKLQQQSFWVKSNNPIDLEWFSKFVDITHYQFIDIGKKLNFS
                     NWRDNLLPASILVGILLLLAIFINRQKDKIKQKLHQINNRLKIVATDSQWNTPLAIFW
                     TLVLCLPSTLMFLAGFVAITYISFENPQEVWTWGLKMAIYWLYFAFLVAMLRPHGIGH
                     IHFNMPQKSNEIFRNILKRSVWIIALLLNTSIFTNLEMGISYDMLGQTMTIFVLILMI
                     WMIVPAFRQAISTYENVTTEEKKESKSILLTIVKLVLLLAPIILIILIALGYYYTALV
                     IIEHLVDSYFAIITWIIIRNILYRAFSISSRRLAYRRLQEKREAAEAKTRAKAEQNDE
                     NPDGVELPFELKEDSIAVSEVKNQMLKLTDFVLWIGLFVLLYWVWSDLITVALYLDGV
                     TLWQQSVTTEAGIVMESITLLNLLTAILIIVVMVVLIRNLSGLLEALIFSHFKLSQGT
                     PYTITTLLTYLIIALGAIFAFGTLGMSWSKLQWLLTALSVGLGFGMQEIFANFVSGII
                     ILFERPMRIGDTITIGTYSGTVSKIRIRATTLVDFDGKEVIVPNKAFVTERLVNWALS
                     STATRIIVQVGVAYGSDLELVKRLLLQVAEENPKVLKDPQPRAYFLTFGASTLDHELR
                     AYVNDLSDRNPTIDALNTRIHQLFNEHNIDIAFNQLDVYIKNNDTQDEIKVDSKRFEF
                     ERPNNITAKEKE"
     gene            2785..3867
                     /gene="aroC"
                     /locus_tag="PXD94_001573"
     CDS             2785..3867
                     /gene="aroC"
                     /locus_tag="PXD94_001573"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL6286424.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGDQRSQDYGDIAEKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYLSQI
                     GNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKEGDSVGAKLTVI
                     AERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTPQGF
                     LSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPCVGI
                     RAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            3886..4713
                     /gene="mepA"
                     /locus_tag="PXD94_001574"
     CDS             3886..4713
                     /gene="mepA"
                     /locus_tag="PXD94_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL6286425.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTHLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQALVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            4786..6705
                     /locus_tag="PXD94_001575"
     CDS             4786..6705
                     /locus_tag="PXD94_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL6286426.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNAVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPIVKKGQILHCEKGEIVSKKTQPPRHFTDRSLLSAMTGIARFV
                     QDKELKKILRETDGLGTEATRAGIIELLFKRGFLIRKGKNIHSTEAGRILIQALPDSA
                     TQPDMTAHWEMQLNQISKKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLAKITPLASG
                     NKPRYFKKRTQTAEN"
     gene            6811..7137
                     /gene="secG"
                     /locus_tag="PXD94_001576"
     CDS             6811..7137
                     /gene="secG"
                     /locus_tag="PXD94_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL6286427.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHNVAPKSQFEDLSQVEQKQVEPVKIE
                     NSDIPQ"
     gene            7162..7247
                     /locus_tag="PXD94_001577"
     tRNA            7162..7247
                     /locus_tag="PXD94_001577"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7196..7198,aa:Leu,seq:gag)
     gene            7450..8691
                     /locus_tag="PXD94_001578"
     CDS             7450..8691
                     /locus_tag="PXD94_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6286428.1"
                     /translation="MARTTKALNKTQIENAKSKDKEYILSDGDGLNLRVKPLPKGSKI
                     WIFNYTNPQTKNRTNLTIGRYPLITLAEARTICDGYRSLIEKGIDPQEEKKRLQQEAE
                     NRVNDTFRNVAESYFNGIYKEKAKNPITREKNWIRLENHIFPYIGDKHVEEIKVKALV
                     DIYSKVADKSNTLKKLHQLVSAVMDHAITQGIIESHNCRLAVKNFYIKPSTPNPTIEL
                     EELPQLFTDLHNAKLSKQTYALVCWSFLTALRPNEAVNAEWKEIDFEKKLWNIPKEKM
                     KGKADKKRPHSVPLSSQALKILEMMKMHSNSSPFVFPGRSSNSKPMSNATVNSTLKRN
                     GYKDRLTAHGIRAFVKTFLSSRKVDRHVSESILSHLLEGGDDLENTYNRYDFLEERVP
                     VMQLLGDYCEQCGMNLELVRG"
     gene            complement(8850..10718)
                     /locus_tag="PXD94_001579"
     CDS             complement(8850..10718)
                     /locus_tag="PXD94_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL6286429.1"
                     /translation="MFKKLLELRQQKAAIEAEMRSILTKADEEKRSTTEAEGAKFDEL
                     RSQAENLVKEIARYEAITDEQRNQPGQQVETANVKVSDDELRHWIKTGELRSLATNTN
                     GGADGGYSVIPELDKQVMKRLTDDSVMRQIANVVKLTQGKEYKKLVSAGGAAVNHIGE
                     GEARTETATPKMNEVTIAVHNIYAYPKTTQEILDFGGVDILGWLTEEISESFVETEET
                     DLTNGTGTKQAKGFLAYPRTTANDKTRPFGTLQKMEVQPATVIGWNSKGQPETLTPYP
                     PYSVNVQRTVNRQNWLTAYIPHSYDENVAETPHFDQWLNFVSDGKQDKAKNILAALYA
                     ILTNRYNWQVFFQVTGKGGSGKSVFAGIATLLAGEKNTASARLENFDDERKIAGLEDK
                     KLIICSEQTKYAGDGGGLKAITGGDMVRVDPKNKHPFNARIAAMIMIVNNDPCKWTER
                     NGGIDRRIVNFRFNKRPPENERDPYFMDKITLEIGGIIRKVLDTFPDPLEAKRALEEQ
                     KESLEALEIKKQSDPLTDFFEYLYTTENTDGLYIGTGNTLGHDKIRTHLYPAYLAFVK
                     AKNVLELGLNTFVVGIEQAVKQHGNKHDFTKRKTNKGQRTNVHFKNFDDFQSDILN"
     gene            complement(10729..11124)
                     /locus_tag="PXD94_001580"
     CDS             complement(10729..11124)
                     /locus_tag="PXD94_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6286430.1"
                     /translation="MISNEAIRQEVIDLLQGNIQGIQHFHNGRAVFTDVENELPAIAV
                     FIDEAEYSQLTLCDGECNAYLKIGIYLPMFSTENDLDLVAQKISDVMNQATFHSMDEC
                     VLAKYSYDYDANESAWKTATLHYNINYLN"
BASE COUNT         3370 a         2351 c         2316 g         3183 t
ORIGIN      
        1 tggtggcagc gaggatttat ctgcgcttta ctctgctcaa ttagatgaaa aaaagcaaga
       61 gcagcaaaat ctacaagcag aaattgcaaa cctgcagact gctatcaaca ataaaattgt
      121 agaagagtct cagaacaagc tagatcaagc acaagcggtt caaaatcagc aaaataatgc
      181 aacgacgaac ccagttatct tgcgtgaatt ggatataaat accaaagtca cacaagagtt
      241 gctaaaacag accaaagata tgacgcagtt atcacaagat aatttgcgaa tcaaaagtgt
      301 gctagataac ttacagcaaa cccaacgtaa tattgaagaa caaatcagtt ctctgcaagg
      361 tacgttggtg ctttctcgta ttattaataa gcaaaaacaa tcattaccac aagatgaaat
      421 gatttccgga ctatcaaaac aaattgcaga tttacgtgta cgtgtttttg atatcactga
      481 atttaaagac tcttttgcgg acataaatgc ttatatcagc cgcatagagc aagatgaaaa
      541 aacaaccttt acgagtaaag agaaagaaca actgagtaaa attttacagg aacgttctga
      601 cactttaact gagatgatta aatcattaaa taatcagctc aatttattaa tcaatattga
      661 actaaaccag caacaagtgc agaccattag tgatgcgtta cagcaaaaac tgcaacagca
      721 aagcttctgg gttaaaagta acaaccccat tgatcttgaa tggtttagta aatttgtcga
      781 tattacccac tatcaattta ttgatattgg aaaaaaactc aatttttcaa attggcggga
      841 taatttatta cctgcaagta ttttagtcgg aattttattg ctattagcca tctttattaa
      901 tcgccaaaaa gataaaatta aacaaaaatt acaccaaatt aataatcgac taaaaattgt
      961 cgccacagat agccaatgga acacccctct tgctattttt tggacattag tgctttgcct
     1021 gccaagtaca ctaatgttct tagccggatt tgttgctatc acttatattt catttgaaaa
     1081 tcctcaggaa gtctggactt ggggattaaa aatggcaatt tattggctgt attttgcctt
     1141 tttagtggcg atgctacgcc cgcacggcat aggacatatt cactttaata tgccacaaaa
     1201 aagtaatgaa atcttccgta atattttaaa acgttcggta tggatcattg ctttattact
     1261 gaacacctct atttttacaa acttagagat gggaatatct tacgacatgc ttggacaaac
     1321 aatgactatt tttgtcctta tattaatgat ttggatgatt gtaccggcat ttaggcaagc
     1381 tatttctacc tatgagaatg tcacaacaga agagaaaaaa gaatctaaaa gtattttact
     1441 cactattgtc aaattagtgc tactgcttgc ccctatcatt cttatcattc tgattgcatt
     1501 gggctattac tatactgcac tggtgatcat tgagcattta gtagattctt attttgcgat
     1561 cattacttgg attattattc gtaatattct ctatcgagca ttctctattt catcacgccg
     1621 tctggcttac agacgtttac aagaaaaaag agaagcagct gaagccaaaa cacgagctaa
     1681 agcagaacaa aatgatgaaa acccggacgg tgtcgagctt ccttttgagc taaaagaaga
     1741 ctctattgcc gtaagcgaag tcaagaacca aatgttgaaa ttaactgatt ttgtgctttg
     1801 gattggctta tttgtgctgc tttactgggt ttggtcggac ttaattactg ttgcactcta
     1861 cctagacggc gttaccttat ggcaacaaag tgttacaaca gaagccggga ttgtaatgga
     1921 atccattacc ctgctgaatt tactcactgc tattctgatt attgtggtaa tggtggtact
     1981 cattcggaac ttatccggtt tacttgaagc cctgattttc tcgcacttca agttatcgca
     2041 aggtacacca tacacaatta ccacactctt aacctatttg attattgcgt taggggcaat
     2101 ctttgcgttc ggtacattag ggatgtcttg gagcaagctc caatggctac taactgcact
     2161 ttctgtcggt ctgggttttg gtatgcaaga gatctttgca aacttcgttt cagggattat
     2221 catcttgttt gaacgtccaa tgcggattgg cgacaccatt accatcggca cttacagcgg
     2281 tacggtttcc aaaattcgta ttcgtgccac aaccttagtc gattttgacg gcaaagaagt
     2341 gattgtacca aacaaagcat tcgtaaccga acgcttggtg aactgggcat taagcagcac
     2401 cgcaacccgc attattgttc aagtcggcgt ggcttacggg tctgatttag aattagtcaa
     2461 acggctgtta ttacaagtgg cggaagaaaa tccgaaagtc ttgaaagatc cacaaccaag
     2521 ggcttatttc cttacctttg gtgcaagcac tttagatcac gaattaagag cctatgtgaa
     2581 cgatttatct gatcgtaacc caactattga tgcactaaat acacgcatcc accagctctt
     2641 taatgaacat aatattgata ttgcatttaa ccaattagat gtttatatca aaaacaacga
     2701 tacacaagat gaaattaaag tagattcaaa acgctttgag tttgaacgac caaataacat
     2761 aacagcaaaa gaaaaggaat aacaatggca ggcaacagca tcggacaatt atttaaagtg
     2821 accactttcg gcgaatctca cggtattgca ttaggctgta tcgtagatgg cgtaccgccg
     2881 aatatggctt taagcgaagc agatttacag ccggatttag accgccgtaa acccggtact
     2941 tcacgctaca ccacgcctcg ccgtgaagat gatgaagtgc aaattttatc gggagtcttt
     3001 gaaggcaaaa cgacgggaac aagcattggt ttaatcatta aaaacggtga ccaacgctca
     3061 caagactatg gcgacattgc cgaaaaattc cgcccggggc acgcagatta tacctatcag
     3121 caaaaatacg gcattcgaga ttaccgtggc ggtgggcgtt cttctgctcg ggaaaccgca
     3181 atgcgggttg ccgccggggc gattgccaaa aaatatttgc gtgagcaatt tggcattgaa
     3241 gtgcgaggct acctctcaca aatcggcaat atcaaaattg acccgaaaac cgttgaaaat
     3301 attgaacaaa ttgattggca cgcagtcaat agtaatcctt tcttctgccc cgatcccgtt
     3361 gcgattgaag gctttgatgc cttaattcgt gagctgaaaa aagagggcga ttcggtgggg
     3421 gcaaaattaa ccgtgattgc agagcgtgta cctgtagggt taggcgaacc tgtgtttgac
     3481 cgcctagatg ccgaccttgc ccacgcctta atgtcgatca acgccgtgaa agcggtggaa
     3541 attggcgatg gttttgcagt tgtggaacaa cgtggtagcc agcaccgaga tgagatgacg
     3601 ccgcaaggct ttctctctaa tcacgccggc ggaattttag gtggtattag ctccggtcag
     3661 ccgattatcg ctcatattgc gttaaagccg acctcaagca ttactattcc cggcagaagc
     3721 gtgaacaaaa acaacgaagc cgttgaagtg atcactaaag gcagacacga cccctgtgta
     3781 ggtattcgtg ctgtgccgat tgcagaagcg atgatggcga ttgtactact cgaccatctg
     3841 ttaagattta aggctcaatg ccgttaaaca cataaaattg gaaaaatgaa attattaaaa
     3901 acacttttag tctcaagcct tgcttttaca gctaccgcac tgaatgcaac gcaatgggag
     3961 aaaatcaaaa ctccggttca aggaaaagca caatctatcg gcggttatag taacggctgt
     4021 attattgggg cacagcccct tgcattaaaa ggcgaaggct accaagtgat ccgctcggct
     4081 cgtaaccgct attacggaca cccacaactg ctcgattatc tcactcactt aggcaaaaaa
     4141 accaaagctg ccggtgtgcc gaccatttta atcggcgata tgggaatgcc tgccggtggg
     4201 cgttttgcct ccggtcacgc ctcccatcaa accgggctgg atgctgatat ttggtttaag
     4261 ttcggaccga tggacgatga aaccgcccgc aatccagcag ggcttgccac cattatggta
     4321 aaccgtagta ccgagcgggt tgatgattca ctctggaatc aacaccatac taatttagta
     4381 aaacttgctg caaccgatcc tcgagtggat cgcatttttg tcaatcctgc cattaaagtg
     4441 aaactctgcc aaaccgcagg caatgatcgt gcttggttac gcaaaattcg cccgtggtac
     4501 ggacacgact cccatttcca cgtccgttta acctgcccgg ctgatgccgc aagttgtgaa
     4561 aatcaagccc ttgtgcctgc cggtgacggc tgtggtgaag agctttactc gtggtttgag
     4621 ccgaaagaag cctccggcac accaaaagcc aaagtgctac ccacagcgcc tgttcaatgt
     4681 cagatgattt tatctgaaca aggcttgaat taattgcaaa ataacaacct tttttgaccg
     4741 cttgtcagcc cacaagcggt tcattttctt aaataattta cgattatgcg tttatttgtt
     4801 gccgaaaaac ccagccttgc ccgagcgatt gccgatgtgt taccaaaacc ccataccaaa
     4861 ggagatggct ttatccaatg cggcgaacac gacactgtta cttggtgtat cggtcattta
     4921 ttggaacagg ctgaacccga tgtctatgat gagcgtttca aaatgtggcg aatggaacat
     4981 ttgccgattg tgcctgaaca atggaaattg attcccaaaa aagagacgct caagcagttt
     5041 aaagtggtgg aaaagctgat taaaaaagcc gatgttttag tcaatgccgg agacccggat
     5101 cgagaagggc agctgttggt ggacgaagtt tttggctatt tgaatttatc tgctgaacgc
     5161 aaaagccaaa ttcagcgttg tttggtaagc gatctcaacc cgaatgcggt gagccgagcc
     5221 attgaaaaat tgcaaaaaaa cagcgatttt ataccgcttg caacctctgc tctcgcccga
     5281 gccagagcgg attggctgta tggtatcaat atgacccgag cctataccct acaggggcgt
     5341 tgggcaggct ataaaggcgt gttatcagtc ggacgtgtgc aaacgccggt attagggctg
     5401 attgttcgcc gagatctgga gatcgagcat ttcgtgccaa aggattattt tgaggtgttg
     5461 gcacagattg tcgtgccgga aacccaagag cgatttaagg cgcagtggca accgagcaaa
     5521 gcctgtgaag attatcaaga tgaagatggg cgggtgctgt ctcgtccatt ggcagaaaat
     5581 gtggtaaaac ggattaccga ccagccggca accgtcaccg aatatcaaaa taaaattgaa
     5641 aaagaaactg caccgctgcc ttattctctt tcggtattac aaatggaagc cgccggcaga
     5701 tatgggctat ccgcccaagc ggtgttggat atttgccaaa agctgtatga aacccataaa
     5761 ctaattacct accctcgttc tgataatcgg tatttgccga ccgaacattt caacgacaga
     5821 tttaaagtga tggcggcgat tgctcatcat ttacctgaat atcaacaaaa accggaagtg
     5881 attgatgcca ctattcgcaa tcgttgctgg aatgataaag aggtggaagc ccaccacgcc
     5941 attattccca ctgcacggca agggacggta catttaaccg aaaatgaaaa aagagtttat
     6001 caactcattg cccgccaata tctaatgcaa ttctgccctg atgcggaata tcgcaaaagt
     6061 aaaatcgggc ttgaaattgc aggtggtagt tttgtcgctc aagcacgcaa ccttgtaatt
     6121 gccggctggc gagcgttact gggtaaagaa gatagccttg acgatgcaga gccactacta
     6181 ccgattgtca aaaaagggca aatcctccat tgtgaaaaag gggaaattgt cagcaaaaaa
     6241 acacaaccgc cccgccactt tactgatcgc agtttgcttt cagcaatgac cgggatcgcc
     6301 cgttttgtac aagataaaga attgaaaaaa atcttgcgtg aaaccgatgg tttaggcacg
     6361 gaggcaaccc gagccggtat tatcgaattg ctgtttaaac gtggtttttt aatccgtaaa
     6421 gggaaaaata tccacagtac cgaagccggc agaatattga ttcaagcact tcccgactct
     6481 gctacccagc cggatatgac cgctcattgg gaaatgcagc tgaatcaaat cagcaaaaaa
     6541 gagaccagct atcagcaatt tatgtttgat ctaaaccaac agctcccaca tttattacaa
     6601 agcccaaacc gccaagtgtt gcaaaattta gcgaaaatca caccgcttgc aagcggcaat
     6661 aaacctcgtt attttaaaaa gcgaacacaa actgctgaaa attaaatcaa gcaggtaaaa
     6721 attactgttt agcacttgtc aaacgggcgt aaattctata ttatgcacgc cattgcaaat
     6781 ttcgcataaa gaatacaaga gaataaatta atgttaatta atgtactcac tattgtttat
     6841 cttgtagttg ccgtattact gatcgcattt gtattaatgc aacaaggtaa aggcgctgat
     6901 gcaggtgctt ctttcggggc gggtgcttca gcaacagttt tcggctcagc aggatcagca
     6961 aattttttat caagaacaac tgctctactt gcaacgattt tcttcgcaat tagtttagtt
     7021 atcggtaatt taagctcaca caatgtagca ccgaaaagcc aatttgaaga cttaagccaa
     7081 gttgaacaaa agcaagtaga acctgtaaaa atagaaaata gcgatatccc tcaataatcg
     7141 cttaacatta aaaagaataa agctctggtg gtggaattgg tagacacgcc atcttgaggg
     7201 ggtggtggac acagtccgtg cgagttcaag tctcgcccag agcaccattc aactaaattt
     7261 ggctcaacgg agctaaacta aaaaagcctt agaaagcctg taaaatcaac atttacaggc
     7321 ttttttacta tcttaactca acggctctaa acgtttccta tcacaaattt tagttacacg
     7381 tttagttaca cgttttaaat cgtgttacta tactttcaat aatcgtgtaa cacttcgtag
     7441 gtttaattta tggctagaac aactaaagca ttaaataaaa ctcaaataga aaatgccaaa
     7501 tcaaaggata aagaatatat tttatccgat ggcgatggat taaatcttag agttaaacct
     7561 ctccctaaag gctctaaaat ttggattttt aactacacta acccacaaac taaaaacaga
     7621 acaaatttaa ctattgggcg ttacccctta attacacttg cggaagctcg cactatatgt
     7681 gatggctacc gctcgttaat agagaaaggc atagatccac aggaagaaaa aaagcggctg
     7741 caacaagaag cagaaaatcg agtaaatgat actttccgga atgtggccga aagctatttc
     7801 aatggtattt ataaagagaa agcaaaaaat ccgattactc gtgaaaagaa ttggataagg
     7861 ttagaaaatc atattttccc ctatatcggg gataaacacg ttgaagaaat caaagtaaaa
     7921 gcccttgttg atatttattc taaagtggca gataaaagca acacattaaa aaaactgcat
     7981 cagcttgtat ctgctgtaat ggatcacgcc attacgcaag gcattataga aagccataac
     8041 tgccgattag cagttaaaaa cttttatatc aagccatcta cgccaaatcc cactattgag
     8101 cttgaagaat taccgcagtt atttactgat ttacataatg cgaagttatc aaagcaaact
     8161 tacgcccttg tttgttggtc gttcttgact gctctacgcc caaatgaagc cgttaatgct
     8221 gaatggaaag aaatagactt tgagaaaaag ctatggaata tccctaaaga gaagatgaaa
     8281 ggaaaagcag ataaaaaacg ccctcactct gtgccgttat cctcccaagc gttaaagata
     8341 ttagagatga tgaaaatgca ctctaattct agcccttttg tttttccagg tcgttcatca
     8401 aatagtaaac caatgagcaa cgcaacggta aattcaacat taaaaaggaa tggatataaa
     8461 gatagactaa ccgcacacgg tattcgggca tttgtgaaaa catttctatc ttctcgcaaa
     8521 gtagatcgcc acgtttccga aagtatatta tcccatttat tagaaggtgg agatgattta
     8581 gaaaacacat acaaccgata tgatttttta gaagagcgtg tgccagtaat gcagctttta
     8641 ggtgattatt gcgaacagtg cggaatgaat ttagagttag ttcggggtta gggaggtaaa
     8701 atatatgatt ttagtcgtca cgtcatcact tttcccactt tcccctttaa aatcaaatag
     8761 ttaagtggtg atgactggtt ttaaagtcat cattaagtca tcacaaaaag gtgatgacct
     8821 agaaaaaaag aggggtttcc ccctctttgc taattcaaaa tatcactttg aaagtcatca
     8881 aaatttttga aatgaacgtt tgttcgttgc cctttattcg tctttcgttt tgtgaaatcg
     8941 tgcttattgc cgtgttgctt tactgcttgc tcaataccta ctacaaaagt atttaagcct
     9001 agttctagca cattcttagc cttaacaaag gctaaatatg ccggataaag gtgcgttctt
     9061 attttgtcgt gtccaagcgt gttgccagtt ccgatataaa gcccgtctgt attttctgtt
     9121 gtatatagat actcgaaaaa atccgttaat gggtcagact gctttttaat ctccaacgcc
     9181 tctaggctct ctttttgctc ctctaaggct cttttagctt cgagtgggtc agggaaggta
     9241 tcaagcactt tacggataat gccccctatc tctaatgtaa ttttatccat aaaatacgga
     9301 tcacgttcat tttcaggcgg tcttttatta aaacggaaat tgacaatccg cctatctata
     9361 ccgccattcc gttctgtcca tttacaaggg tcgttattga cgatcataat cattgccgca
     9421 attcttgcgt taaatgggtg cttgttcttc ggatcgactc gcaccatatc accgccagta
     9481 atcgctttca atccgccacc atctccagcg tatttcgttt gctcggaaca gataatcagc
     9541 tttttatctt ccagccctgc gattttgcgt tcatcatcaa agttttctag ccttgctgaa
     9601 gctgtgttct tttctccagc taatagtgtc gcaatgccag caaataccga tttaccgctt
     9661 ccgcctttcc ctgttacttg gaagaatact tgccagttgt agcggtttgt taaaatcgca
     9721 tagagagcgg ctagaatgtt cttcgccttg tcttgcttgc cgtctgatac aaagttaagc
     9781 cattgatcaa agtgtggtgt ttcggctacg ttctcgtcat agctatgtgg aatataagcc
     9841 gttagccagt tttggcggtt aacggttctt tgaacattta ccgaataagg tgggtaaggg
     9901 gttaaagttt cgggttgccc cttgctattc caaccaatca ccgtagccgg ttgtacttcc
     9961 attttttgaa gcgtgccgaa tggtcgggtt ttatcgttgg ctgtcgtgcg tggataagct
    10021 aagaagcctt tagcttgttt tgtgcctgta ccgttggtta aatcggtttc ttcagtttcg
    10081 acaaaggatt ctgaaatttc ttcagtcagc cagcctagaa tatctacacc accaaagtct
    10141 aaaatctctt gcgtggtttt cgggtaagcg taaatattat gaacggcaat cgttacttca
    10201 ttcattttcg gggtggcggt ttcggtgcgt gcttcgcctt caccgatatg atttaccgct
    10261 gcaccgccag ccgatactaa ttttttatat tctttgcctt gggtcagttt caccacgtta
    10321 gcaatttgtc gcataacaga atcatctgtt aggcgtttca ttacttgctt gtcaagttcc
    10381 ggaatcactg aatagccacc gtctgcccct ccatttgtat tcgtagcaag cgaacgcaat
    10441 tcgcctgttt taatccagtg gcgaagctcg tcatcagata ctttcacatt tgcggtttca
    10501 acctgttgcc ccggttggtt acgctgttca tcagtaatcg cttcataacg tgcgatttct
    10561 tttactaaat tttcagcttg tgagcggagt tcatcaaact tagccccttc tgcttcagtt
    10621 gttgagcgtt tttcttcgtc tgctttggtt aaaattgaac gcatttctgc ttcaatagcc
    10681 gctttttgtt ggcggagttc taaaagtttt ttaaacattg ccttatcctt aattcaaata
    10741 attgatattg tagtgaagcg ttgctgtttt ccacgctgat tcgttagcat cataatcata
    10801 gctatactta gccagcacac attcgtccat actgtggaat gtggcttgat tcatcacatc
    10861 actgattttt tgagccacta aatctaggtc gttttcggtt gaaaacattg gtaagtaaat
    10921 gccgattttc aaataggcgt tgcattcgcc atcgcaaagc gtgagttggc tatattccgc
    10981 ttcatcaatg aatacggcga tagccggtaa ttcattttct acatcggtga atacggctcg
    11041 gccgttatgg aaatgttgaa tgccttgaat gttaccttgt aataagtcga taacttcttg
    11101 gcggattgct tcgttagaaa tcataaaaaa gtcctcttaa ttaagaaggc tacaattagc
    11161 cacagaacaa tataggcata atttcaacgg agtaaatagg gtaaaattta atcgtttagc
//
LOCUS       DANLXQ010000080        11219 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.080, whole genome shotgun sequence.
ACCESSION   DANLXQ010000080 DANLXQ010000000
VERSION     DANLXQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.080"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            145..363
                     /locus_tag="PXD94_001581"
     CDS             145..363
                     /locus_tag="PXD94_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286431.1"
                     /translation="MYQYYLAQVGNEQQKLIRGMPDNLLSFSVYEPEQEDWDNKFGPF
                     WADKILISGFDAYEEISEKEAIRFIKVH"
     gene            364..777
                     /locus_tag="PXD94_001582"
     CDS             364..777
                     /locus_tag="PXD94_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL6286432.1"
                     /translation="MCQAEQFARQLHYGQTDKAGKPYIEHLAFVAENISTKNEETEAV
                     AWLHDSVEDTQCSINDIRHIFGDVIADAVEAITKKQGESYFCYLDRVKSNPIAKVVKL
                     ADLTHNLCLERLPKITEKDLKRAEKYKSALEYLQA"
     gene            895..1629
                     /locus_tag="PXD94_001583"
     CDS             895..1629
                     /locus_tag="PXD94_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286433.1"
                     /translation="MKLKLDENGHVVVENGMPVYIHDDGKEIPFDALKATQKITQLNA
                     EAKQHREAKEKAEADLKAFSGIDDPKAALEALKTVANLDAKKLIDAGDAEKVKAEIIK
                     GYDEKLAVATAQAEKLQQQLHAELIGGSFARSKYAQEHLNIPSDVVQAFFGKHFSISD
                     DGKVVAKFADGGEIFSRTRPGEKADFEEALEALVSAYPNKDAILKPSGSSGAGAGGGS
                     RSSSMPKTLSDCKTDEERIAYLQANS"
     gene            1695..2642
                     /locus_tag="PXD94_001584"
     CDS             1695..2642
                     /locus_tag="PXD94_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286434.1"
                     /translation="MAFDLQVFNKQTQLALTETVDQAIEKFNAASGGAIILQNKPAEG
                     DFDIRASFKGIAGLVRRRNVYGTGTVDAKRLEQLLDVAVKVAAGTAPIEYQPSQYKWI
                     LLNPELAAIEIGTQLAKARVADMLNTAIAGTVAAISGNTAMTLDEKTAAPTFRILNKG
                     AAKMGDRAGSLKAWVLHSTTLHGLYDNALTNAENLFKYENVNVIRDPFGRVFVVTDSP
                     ALMASDGTFNTLGLVENAVVVSGNNDFDSVLVPKTGGENLSAVYQAEWTYNLGILGYK
                     WDMTAGGKSPNDTALGTPTNWDKSVTSNKDTAGVLVKTK"
     gene            2702..3052
                     /locus_tag="PXD94_001585"
     CDS             2702..3052
                     /locus_tag="PXD94_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286435.1"
                     /translation="MKRILYFTTDFSAKNIAFAKAHGVLMRNLNAYHPSDTLENADAV
                     CGNVPQAYQHLPLFELPESNQKSGQDLKTLKTEEIKEKLTELGIAFEANAKKETLLTL
                     LTQAVGRDGTNDAK"
     gene            3042..3479
                     /locus_tag="PXD94_001586"
     CDS             3042..3479
                     /locus_tag="PXD94_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286436.1"
                     /translation="MLNSYMTVEQANAYHDIRMSREAWNALSDQEKQQRLVSASDFLD
                     FNYRFSGEKADLTQEREFPRKGFEAIGIPKAVQFAVCELALQDNLTANQEAQMSSVRV
                     GPVSVNYDNSSPQNSVNRFEYVRQLLSTWLDRSSFGTVKLERS"
     gene            3479..3850
                     /locus_tag="PXD94_001587"
     CDS             3479..3850
                     /locus_tag="PXD94_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286437.1"
                     /translation="MADFYGNLGKISHSLIKQFGQPCSVTINEQGRYNPATGERKKGS
                     STVHAGYCLFDNLAFDFRNQQSVSVQQGDVMIYLTADCKPVLNAVIEANGEKWSVINV
                     QPIRPAATTMIYQVQGRKADG"
     gene            3843..4256
                     /locus_tag="PXD94_001588"
     CDS             3843..4256
                     /locus_tag="PXD94_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL6286438.1"
                     /translation="MGSFHAQIARFVEQTQQKADRALRVIALQTLSGVQKKSPVDTGQ
                     LRRSWTVALNGFPSSYNGSQTVLNSAKFGDMIVIATNKPYAPMLEYGLYPNPPKKPTG
                     KTKNGYSIQAPNGMVRITVQEMQAFIRNNPQLGVS"
     gene            4256..4648
                     /locus_tag="PXD94_001589"
     CDS             4256..4648
                     /locus_tag="PXD94_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286439.1"
                     /translation="MRPIIRSVLEQHLAELNPFNTVWEGIIDSPKLPYQTVSMTTSTN
                     ETATIGNTPKAKQSGFLQVTLYYPLDQGTKAIEERADEIFRHFFGQVWIEENVQILVQ
                     QPPLVASLYRLDNSIALPITIYFTAYEL"
     gene            4658..5680
                     /locus_tag="PXD94_001590"
     CDS             4658..5680
                     /locus_tag="PXD94_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286440.1"
                     /translation="MSILGNSQGTKRTVVFAKESSFGTKAAPTGAKIMPRVETSLNTN
                     FESYQSEEIRDDLQRSASVIGFEKVEGDIKGELAAGQWAAFFAAALRGSFTAQAKKPI
                     ITKTTDGTGEKNGKILIVPKTSHTSDSFTLEDVFKDINVSRQYLGCRVSKLSLDVQPN
                     GIASITVSFLGQRGEESTTAYFTNPTDLTQSGKLAGVKGSLQVNKRPVGLVTAFKLDI
                     DLGASSEPVLGAKYAPDVFIGTVAVSGSFTMYFQDKTMIDAVRNGTDLSLALRMDAEE
                     RTNTDYLTIILPGVKVTSNELDDGAKNIMQTLNFDAFPAVWDSASQIDNVLKVATTMI
                     VQDSLA"
     gene            5770..6171
                     /locus_tag="PXD94_001591"
     CDS             5770..6171
                     /locus_tag="PXD94_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286441.1"
                     /translation="MNLNALSKEKQLAEHRFELVHPVSGEPLGVFMSVVSAKSDKAQR
                     FAEKQLRQEQMRELENAKSRKPKFKGLGDMIAESRAMAIDRLTGWENVEWNEKPLEFS
                     DQNAELLLTECDWIVEQIFEHSNDLGKFLKS"
     gene            6186..6512
                     /locus_tag="PXD94_001592"
     CDS             6186..6512
                     /locus_tag="PXD94_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286442.1"
                     /translation="MYAEKTFKLDKKLKGSESTLREHLQAIEMQTGITPEELHNPEPS
                     PAVAYLLGWFFELAMSRQSGMSLNPISYTEIDAWSRLFARHLEQWEISVIKQLDMIYL
                     NVQQTE"
     gene            6514..6837
                     /locus_tag="PXD94_001593"
     CDS             6514..6837
                     /locus_tag="PXD94_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6286443.1"
                     /translation="MDTFNFQPNWGLNVQRKPEVMKIAFGDGYEQVAPKGLNHNLRTY
                     SAVFTGTEARIKQIEAFFERQGGYKAFLWTPYGGKEGKFRCEEWEVSISSGKWTLSAT
                     IREVL"
     gene            6924..7616
                     /locus_tag="PXD94_001594"
     CDS             6924..7616
                     /locus_tag="PXD94_001594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286444.1"
                     /translation="MKKIFVSALLLTCSLSALGNSSYELIIDSMQDLEKHKPEILSIP
                     EADRNTFIRYMMRKELRIKLNQGRLADGLTVREAIQNQQAFEKDRNSKMQQEKQKKET
                     EKAEKEKAVTDFNQKFELSITGARKAKNALNQDILLVSLSLKNNSDKPIIAVQPDIQF
                     VLGEDKQTISSMRPKKFENGIPPNGSEIFEFGFDLDDMFALKAEKNIEKLRAVFQTAE
                     VLFADGTTEKLQ"
     gene            7680..8504
                     /locus_tag="PXD94_001595"
                     /pseudo
     CDS             7680..8504
                     /locus_tag="PXD94_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            10548..10706
                     /locus_tag="PXD94_001596"
                     /pseudo
     CDS             10548..10706
                     /locus_tag="PXD94_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            10714..>11219
                     /locus_tag="PXD94_001597"
     CDS             10714..>11219
                     /locus_tag="PXD94_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6286445.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRNLKDKSGNAGRVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEASGFERNSSGPSNRPTLTLSNLFGLITSIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNA"
BASE COUNT         3529 a         2201 c         2637 g         2852 t
ORIGIN      
        1 cggtaaattg cacgagctct atgaaaaatt cagagatctg aatatacctc ttgcaaaaaa
       61 atcagaaatt ttacttgaaa tgcagaagtt aagtgctaat ctaaaagagc caaagaacat
      121 tgatttaatc aaggagaaaa aataatgtat cagtattatt tagctcaagt tggcaatgaa
      181 cagcaaaaac ttattcgtgg tatgcctgac aatctactta gtttctcggt ttatgagcct
      241 gaacaagaag attgggataa taagttcggc ccattttggg cagacaagat cttgataagc
      301 ggatttgacg cttacgagga aatttcagaa aaggaagcaa tccgatttat caaggtacat
      361 taaatgtgtc aagctgaaca attcgctcgc caactgcatt atggacaaac agataaagct
      421 ggaaagccat acattgagca tcttgctttt gttgcagaaa acattagcac aaaaaatgaa
      481 gagacagaag cagtagcttg gttgcacgac agtgtagaag atactcagtg ttcaattaat
      541 gatattcgtc atatatttgg cgatgttatt gctgatgcag ttgaggctat tactaaaaag
      601 caaggcgaaa gttatttttg ctacttggat agagtaaaat caaatcctat tgctaaggtg
      661 gttaagttag ctgatttgac tcataatctt tgccttgagc gattgcccaa gatcactgaa
      721 aaggatctta aacgagcaga aaaatacaaa tcagcattgg aatatctaca agcctagcct
      781 aaaacgctag gcttttttat cgcaattttt taccccaaac tgaccgtttg tgagccatca
      841 caggcggttt tttattatcg ggcggaagcc caccagcaat gagaggaagc tcaaatgaaa
      901 ttaaaattag acgaaaacgg acatgtagta gttgaaaatg gtatgcctgt gtatattcac
      961 gatgacggaa aggaaattcc gttcgatgca ctaaaggcga cacagaaaat cacacaactg
     1021 aacgctgagg caaagcaaca tcgagaagct aaagagaaag cggaagccga cttaaaagca
     1081 ttctcaggga ttgacgatcc gaaagctgca ctcgaggcat taaagacggt tgccaattta
     1141 gatgctaaaa agctgattga tgccggtgat gcggaaaaag tcaaagcgga aatcatcaaa
     1201 ggttacgatg agaaattagc tgtggcaacc gctcaagccg aaaaactgca acagcagtta
     1261 cacgctgaat taatcggtgg ttcgtttgct cggtctaaat acgctcaaga acacttgaat
     1321 attccgtctg atgtcgtaca ggcattcttc ggtaagcatt ttagtatttc ggatgacggc
     1381 aaagtggtag ctaaatttgc tgacgggggc gaaatcttta gtcgcacccg cccgggcgaa
     1441 aaagcagatt ttgaagaggc attagaagct ttagtaagtg cttatccaaa taaagacgct
     1501 attttaaaac caagtggttc aagtggggca ggagcaggtg gcggatcacg cagttcatct
     1561 atgccgaaaa cattatcgga ctgcaaaaca gatgaagagc gtatcgctta tcttcaagca
     1621 aattcgtaag gtgcaagagg ttgcaccttt tttattttcg gtgcaatcgc accacttttt
     1681 agaggaaaat caaaatggca tttgacctac aggtttttaa caaacaaacg caattagcct
     1741 tgaccgaaac tgtcgatcag gctattgaaa aattcaatgc agcatcaggc ggtgcaatca
     1801 ttttacagaa caagccagct gaaggtgatt ttgatatccg tgctagcttt aaaggtattg
     1861 ccggtttagt tcgccgccgt aatgtgtacg gtacaggtac ggtagatgca aaacgtttag
     1921 agcaattact ggacgtagca gttaaagtgg cggcaggtac agcaccgatt gaataccagc
     1981 cgagtcaata taaatggatt ttattaaatc ctgaattggc ggcgattgaa atcggcacac
     2041 aacttgccaa agcccgtgtt gctgatatgc tcaatacggc tattgccggc acggttgctg
     2101 caattagtgg caatacagca atgacacttg atgaaaagac cgccgctccg acattccgca
     2161 ttttaaacaa aggtgcagcg aaaatgggtg atcgtgccgg ttcgcttaaa gcgtgggtgt
     2221 tgcactccac aaccttacac ggcttatacg ataatgcctt aaccaatgcg gaaaacttat
     2281 tcaagtacga aaacgtgaac gtaattcgtg acccgtttgg gcgtgtattt gtcgtcacag
     2341 acagcccggc tttaatggct tcagacggta catttaacac cttaggcttg gttgaaaatg
     2401 cggtggtagt ttccggcaac aatgactttg acagcgtgtt agtgccgaaa accggcggag
     2461 aaaacctttc agcagtttac caagctgaat ggacctataa cttaggaatc ctaggttata
     2521 aatgggatat gaccgctggt ggtaaatcac cgaacgatac cgccctaggc acaccgacga
     2581 actgggataa atcggttacc tccaacaaag acaccgccgg tgtattggta aaaacgaagt
     2641 aatttttaac cgcttgtaga taaggtttac aagcggttgt ttttttatct ggagtttgca
     2701 aatgaaacga atactttatt ttacaacgga tttttcggca aaaaacattg cctttgccaa
     2761 agctcacggt gtattaatgc ggaatctgaa cgcttaccac ccatcagaca ccttagaaaa
     2821 tgcagatgca gtgtgcggta acgtacctca agcttatcaa catttaccgt tgtttgaatt
     2881 gcctgagtct aaccaaaaat caggtcaaga tttgaaaacc ttaaaaaccg aagaaattaa
     2941 ggaaaaactg actgaattag gtattgcttt tgaggcgaat gccaaaaaag aaacattact
     3001 aaccttactc actcaggcgg tgggacgtga cggaacaaac gatgctaaat agttatatga
     3061 cggttgagca agccaacgcc tatcacgaca tcagaatgag ccgtgaggcg tggaatgcgt
     3121 taagcgatca agaaaaacag caacgattgg tgtcagcgtc tgattttctg gatttcaatt
     3181 atcgtttttc aggcgaaaaa gcagatctta ctcaagagcg tgaatttcca cgcaagggtt
     3241 ttgaagctat cggtattcct aaagccgtgc aatttgcggt atgtgagttg gcattgcaag
     3301 ataacttgac cgcaaatcaa gaagctcaaa tgagtagcgt aagagttggc ccggtttcag
     3361 ttaattatga taacagttca ccgcaaaaca gcgtgaatcg gtttgagtat gtgaggcagt
     3421 tgctttccac ttggttagat cgatcttctt tcggcacggt taagttggag cgtagttgat
     3481 ggcggatttt tacggcaatt tagggaaaat ctcccattct ttgattaagc aattcgggca
     3541 gccttgtagt gtaacaatta acgaacaagg cagatacaac cctgcaaccg gtgagcggaa
     3601 aaaaggtagc tcaacggttc acgcaggcta ttgtctgttt gataatttgg cgtttgattt
     3661 ccgcaatcag caatctgtca gcgttcaaca aggcgatgtg atgatttatc tcactgccga
     3721 ttgcaaaccg gtattaaatg ctgtgattga agcaaacggc gaaaaatggt cggtcatcaa
     3781 cgtccagcct atacgcccag cagctaccac gatgatttat caagtacaag ggagaaaagc
     3841 cgatgggtag ttttcacgct caaatcgccc gttttgtgga acaaacgcaa caaaaagcgg
     3901 atagggcgtt aagggtaatt gccctgcaaa ccttaagtgg ggtgcagaag aaatcgcctg
     3961 tcgataccgg acaacttcgg cgtagctgga cggtggcatt aaatggtttc ccgagcagtt
     4021 ataacggcag tcaaaccgtg ctgaatagtg ctaaattcgg cgatatgatt gtgattgcta
     4081 ccaataaacc ttatgcacca atgctcgaat atgggcttta cccgaaccca ccaaaaaaac
     4141 cgaccggcaa gaccaaaaac ggttattcaa tacaagcacc taatggaatg gtgcgaatta
     4201 ccgtgcagga aatgcaagcc tttatccgca ataatccaca attaggagta agctgatgcg
     4261 accgattatc cgctcggtat tagagcaaca tcttgccgaa ctcaatccgt ttaatacggt
     4321 ttgggagggc atcatcgaca gcccgaaact gccgtatcaa accgtatcaa tgacgacttc
     4381 aaccaacgaa accgccacga ttggtaatac gccgaaagcc aaacagagcg gttttttgca
     4441 ggtcacgctt tactatccgc tagatcaggg gacaaaggcg attgaggaac gagccgatga
     4501 gatcttccgc cactttttcg ggcaggtctg gattgaggaa aatgtgcaaa ttctggtgca
     4561 acagccaccg cttgtagcct cactttatcg actggataac agcattgcct tacccatcac
     4621 catctatttt accgcttatg aactatagga gaacccaatg tctattctag gcaattcaca
     4681 aggcacaaaa cgtacggtcg tttttgccaa agagagttct ttcggcacga aagctgctcc
     4741 gacaggcgca aaaattatgc ctcgtgttga aacctcactc aataccaatt ttgaaagtta
     4801 tcaatcggaa gagatcagag atgatttaca acgctccgcc tctgtgatcg gctttgaaaa
     4861 ggtcgaaggc gatattaaag gcgaactagc cgccggacaa tgggcggcat tttttgcggc
     4921 tgcattacgt ggttcattta ccgctcaggc gaaaaagccg attattacca aaaccacaga
     4981 cggcaccggc gagaaaaacg gtaaaatttt gattgtgccg aaaaccagcc acacctccga
     5041 cagtttcacg ctggaagatg tcttcaaaga catcaacgtc agccgccagt atttgggatg
     5101 tcgtgtatcg aaactcagcc ttgatgtgca accaaacggt attgcctcga ttacagtctc
     5161 atttttaggg caacgaggcg aagaaagtac aacagcctat tttaccaatc ccaccgattt
     5221 aacccaatcc ggcaagctgg caggggtaaa aggctcattg caggtcaata aacgcccagt
     5281 cgggttagtg acggccttta aactggacat tgatttaggg gcaagcagtg agccggtatt
     5341 aggggcaaaa tacgcccctg atgtctttat cggtacagtg gcagtgagcg gatcgtttac
     5401 gatgtacttc caagacaaga cgatgattga tgcggtgcgt aacggcacag atttatccct
     5461 tgctttgcgg atggatgccg aagagcgtac caataccgac tatttaacca ttatcttacc
     5521 gggtgtcaaa gtgacctcaa acgagttgga cgatggggcg aaaaacatta tgcagacact
     5581 caattttgac gcattccctg cggtatggga ttcagcaagc caaatcgaca acgtgctaaa
     5641 agtcgccacc acaatgattg tgcaagacag cctcgcttaa tcgaaatttt gcaaaaaaat
     5701 aaccgtatct aaccgcttgc cctcaaacaa gcggtctttt ttatccccaa tttacaaaag
     5761 gaaacccaaa tgaatttaaa cgcattaagt aaagaaaaac agttagccga acaccgtttt
     5821 gagctggttc accctgttag tggcgaaccg ctcggcgtgt ttatgagcgt cgtgagtgct
     5881 aagagtgata aggctcaacg ctttgccgaa aaacaattac gccaagaaca gatgagagag
     5941 ctggaaaatg ccaaatcccg caagccgaaa ttcaaaggct taggcgatat gattgccgaa
     6001 agtcgtgcaa tggcgattga ccgcttaacc ggctgggaga atgtcgaatg gaacgaaaaa
     6061 ccgttggagt tttccgacca gaatgcggag ctacttttaa ccgaatgtga ttggattgtg
     6121 gagcaaatct ttgagcattc gaacgatctg ggaaagttct tgaagagcta attgatgatt
     6181 tacaggtgta tgcagagaaa acgtttaagt tggataaaaa acttaaaggc agcgaaagta
     6241 ccttgcgaga gcatttgcag gcaatcgaaa tgcaaactgg cattacacca gaagaattgc
     6301 ataaccctga acccagtcca gcggtggcct atctgctggg ctggttcttc gagttggcaa
     6361 tgagccgaca atcgggtatg agcttaaacc caattagcta cactgaaatt gatgcgtgga
     6421 gcaggctttt tgctcgtcat ctcgaacaat gggagatcag cgtaattaag cagcttgata
     6481 tgatttactt gaacgtacaa caaacggagt agtatggata ctttcaactt tcagcccaat
     6541 tgggggctta acgtacagag aaagccagaa gtgatgaaaa tcgcctttgg tgacggttac
     6601 gaacaagtcg cccctaaagg gttaaaccat aatctccgca cttattccgc tgttttcacc
     6661 ggcacagaag cacggattaa acagattgag gcattttttg aacgacaagg cggctataag
     6721 gcttttcttt ggacacctta cggcgggaaa gaggggaaat tccgctgtga agagtgggag
     6781 gtaagcataa gcagcgggaa atggacgctg tcggcgacga taagagaagt gttgtagttt
     6841 tttctgattt tgtgaactgg ttcacactct gtgggttgga tataggttag aatttaagtc
     6901 taatttgctt aacggagtct tttatgaaaa aaatttttgt ttcagcctta ttgctcactt
     6961 gctcgttatc tgcacttgga aattcctcgt atgagttgat tattgactca atgcaagatc
     7021 ttgaaaagca taaacccgaa attctatcta taccagaagc ggatagaaat acttttattc
     7081 gttatatgat gcgaaaagaa ctgcggataa agttaaatca aggcagatta gcggatggtc
     7141 taactgtgag agaagctatt caaaatcaac aggcatttga aaaagataga aatagcaaaa
     7201 tgcagcagga gaagcaaaaa aaagaaacag agaaagcaga aaaagaaaaa gctgttacgg
     7261 attttaacca aaaatttgag ctatccatta caggggcgag aaaggcaaaa aatgcactaa
     7321 atcaagatat tttgcttgtt tcattaagtc taaaaaataa ctcagataag ccaattatcg
     7381 ctgtacaacc ggatattcaa tttgtactgg gtgaagataa acaaactatt tcatccatgc
     7441 gtccaaaaaa atttgaaaac ggcattccgc ctaatggctc agagattttt gagtttgggt
     7501 ttgatttaga tgatatgttt gctttgaaag cagaaaaaaa tatagagaaa ttgagggcag
     7561 tcttccaaac agcagaagtt ctttttgctg atggaacaac tgaaaaactt caatagtaat
     7621 tggtaaatct tcattggccg cttgtagaaa tacaggcggt ttttttattg gagcaagcta
     7681 tggcagattt agcaacactg gcgattaaaa tcgaaacaga aggcgcaagc cgagcaagtg
     7741 cagatctaag taaggtcgag caatctgcaa agaacacaga gaaagccgca gataatctga
     7801 cgcgcgcaat gggaaatttg aagcggattt tagcgattgc agggctggct ggtgggttat
     7861 ccgcttacct agatatggct gaaagaatgc agtcgttaaa cgcacagctt aaatttgtta
     7921 ctaactctca atacgaattc aatagcgcac aaaaagagct ttttgcgatt gcgcagaata
     7981 cgcgcgctag tttggaaagc accacgcaac tttatattaa atcttctcag gcactgaaag
     8041 attacggcta cgcgcaaaag gatattctca catttaccga aaccattaac aaagcaatgg
     8101 cagttggcgg ggttaagcca caagagcaag ctagtgcttt actccaatta tcacaggcat
     8161 taggttcagg gaaactgcaa ggagatgaat tccgttcaat ttcagaagct gcaccgatta
     8221 ttttagatac gcttgccgag tatatgggta aaagtcgcga tgaagtaaga aaactcgcat
     8281 ccgagggtaa gctcaccgca gacttactgt tcaatgcgtt taacggttca agcgctaaaa
     8341 ttaccgaaaa atttaatcaa atgccgctaa catttggcgg tgcaatgcag cagttgcaga
     8401 atgccacatt gaaatttatc ggtgacctag atagtgcaaa tggcttgagt ggaatgttag
     8461 ctcaagccat tagtttttta gcacagaatt ttgatgtgtt aggcaaggct ttgatttatg
     8521 caaccggtgc ttatattgcg tttaatgcag ttgcctttgc tcagaatttt gcaaaagcaa
     8581 ccggtggtgt cggtttatta agtagtgcat ttggttattt gactacgatg atccgaggtg
     8641 caactgttgc aatgatggct aatcctatcg agcttttaac aactgcaatt attggggctg
     8701 cttttgtatt tgatgctttt atcagtgata tagaggtagg tgcagcaacc tttgatgcga
     8761 cttggggaga tgttgcgaca gctgtgtggg atgattttaa agatttaact tcagatgcgg
     8821 cagattggtt agtcactcaa tgggataaag caaccgccag tgcagcgagt tcgttcagtt
     8881 ccttaggtgt tgatattggc tcagtatggg agacggtgca atccattacg aaaacattga
     8941 tcaatagtat tattggtctg tttgttggag cttatcaagc catcgtgctt gcttggaata
     9001 attttcctgc cacaatggaa aacttgggtg tgatggcggt aaatgctctt gtcgatttgg
     9061 ttcaaaaagg catcaatagc attattcagc ttatcaaatt accgattgag cttttaaaca
     9121 gagcctcatc tacttttggt ggaggaaatc tgattgatac atcaggttgg aaggcaagct
     9181 tagatgattt taaactaaag gcaagccgcg aggcagaagt tgttggtcaa tcaattagcc
     9241 aagcatttgc ggatagtatc accacagatt acataggcaa tgcagttaat tcagtgagtg
     9301 actatattga tggagcggca gttcgtgggc gcttaaaacg tcaattagat gaaaccgcct
     9361 ctgacgatca aggtacgcag tcaccagcta aaaaaacatt agctggaaat aataactctg
     9421 ctagtgactg gaaaaactac ctcaatgaac ttgaacgagc caatgccgat ggattagccc
     9481 gtattgcact tgagcaagat cgctcaatgc gtgaaatgtt ggaaaaggcg aagaaagccg
     9541 gtgcaagtca cgctgaaatt gaaaaagcca aaatgctgat tacagaacgt tatgctaaag
     9601 agcgaatgga aattgccgag aagtatgtgc cgagtctgaa gtttgaggaa gaactcaaag
     9661 atcagattaa ggaaattgat gaactgcaaa agttaaacct gattaccacc gagcaagcag
     9721 gtcttgcgaa agaggcgtta gcaggcaaat atcagccggt agttgaaatc caacagcaat
     9781 acatcaatca actgagagag attgaagcat taaaacaagc cggtgtatta agcaaacctc
     9841 aagcagaagt agcggccgaa aaagccaaat gggacgccgg cacacaaaaa gccaaaatcg
     9901 caggtaaaaa cgccgttagc ccttatgaaa aatggaaatc ggaatttgac ccaatgcagg
     9961 caattcaaaa tgagcaagcc tcaaagttgg cagaagtgca atcgatgtac gatcagcatt
    10021 tgatccaata tgaggatttt atcaacgcta aagcacaaat tgatgctaag gcgactgctg
    10081 atactcatca actgtttatg aacagtatta gtgggtttgg ttcggcgata gatacgatgt
    10141 taggcgtgat gaaaaacgca gggcaagagc aatcaggtat ttaccgcacg atgtttgcga
    10201 tgtcgaaagc gtttgctatt gcggaatcaa ttctgaactt gaatcgtgca attactcaag
    10261 caatgagcga tcaaacagct attacaccgg ctcaaaaatt tgcaaatatg gcggctattg
    10321 ctagtgccgg ggcgagcttg atttctaata tccagtcagt ctcattaggc ggtatggctc
    10381 acagcggtat tgacaatgtg ccgaaagaag gcacttggtt gctggataaa ggtgagcgtg
    10441 tagtggatag tcgtactaac caagacttaa aaaccttttt atccaatgct aatcgtggcg
    10501 gtgcaagtgg tcaaagatcg gcgataaatg tcaaaattat taataacggg caacccgtag
    10561 aggcgaaagt cagcagtgaa gagactgtag atggcaatat ccaaatgacg gtggaactgc
    10621 tgaagaaaat ggatcaaatc gccgatcaac gctaccgtag aaatcagtta aatgatttaa
    10681 gaacaggtgg aacattatct aaataggtga agtatgccaa ttagtatttc taaccaaatg
    10741 aaactagatc ttgccaaact tgagcaaaat gcaatgctgg acttgtacga ggttgatttg
    10801 cgcaatctca aagataaaag tggtaatgct ggcagagttt accgctttta ttctgggcta
    10861 aatgaactca aaacaagtat cgtctggcaa gggcggactt acgatccgta tcctattgag
    10921 gcaagtggat ttgaacgaaa cagtagcggc ccgagcaatc gcccgactct cacgctatca
    10981 aatctgtttg gtttaattac cagtattgcg aatcagtttg acgaatgtat cggtgcgatt
    11041 gtccgccggc atcaggttta tgctcaatat ttagatgccg tgaattttgc cgggggtaat
    11101 gcgaaagctg atccaaatca ggaaataatc agtcattttg tgattgagca gcttaccagc
    11161 ctcacccgag aaacggcgac ctttacgctg gcattaccga ttgaaaccga taatgccaa
//
LOCUS       DANLXQ010000081        11214 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.081, whole genome shotgun sequence.
ACCESSION   DANLXQ010000081 DANLXQ010000000
VERSION     DANLXQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.081"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            2..1798
                     /locus_tag="PXD94_001598"
     CDS             2..1798
                     /locus_tag="PXD94_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
                     /protein_id="HDL6286446.1"
                     /translation="MDFLTEILLFLKDQVLNKAPFLLGIVACLGYILLKKDTSTIIKG
                     TIKTIVGFMIVQAGSGFLVTNFKPVIDGLAKYHQLTGAVIDPYTSMQSTIQTMGENYA
                     WVGYAVIGALFLNILLVVFRRLTGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETII
                     YTSVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKDDTVD
                     KMKLPNWLNIFHDSISATALVMTIFFGIILLSFGLDNLKELAGKTNQFMYILEMGLKF
                     AVAIQIIVTGVRMFVAELSEAFKGIAERLIPNSVLAIDCAAIYAFSPNAMVFGFMWGA
                     IGQFVAVGGLLAVGSPVLIIPGFIPMFFSNATIGVFANQFGGWKAVMKICFVMGIIEV
                     LGSAWVIQMLASQGTTFNGWMGMADWALFFPPILQGIISIPGFFFVIVAAAMVYMFFA
                     SKQLRAEEAEAAARGITLEQLDGYGLDEVKAEPKAEAVVQQAVENAENSANPIRILAV
                     CGSGQGSSMMMKMKIKGYLDKRGIPNIMDSCAVTDYKGKLSEVDIIVSSKHLANEIEV
                     PEGKAVLGVQNMLNPNSFGDELVELINQHNAK"
     gene            1878..2939
                     /locus_tag="PXD94_001599"
     CDS             1878..2939
                     /locus_tag="PXD94_001599"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL6286447.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            2927..3391
                     /locus_tag="PXD94_001600"
     CDS             2927..3391
                     /locus_tag="PXD94_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL6286448.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            3422..3538
                     /locus_tag="PXD94_001601"
     CDS             3422..3538
                     /locus_tag="PXD94_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL6286449.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            3554..4021
                     /locus_tag="PXD94_001602"
     CDS             3554..4021
                     /locus_tag="PXD94_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL6286450.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            4076..4756
                     /locus_tag="PXD94_001603"
     CDS             4076..4756
                     /locus_tag="PXD94_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL6286451.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRVLHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(4824..5966)
                     /locus_tag="PXD94_001604"
     CDS             complement(4824..5966)
                     /locus_tag="PXD94_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL6286452.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATPEM
                     VKMLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISALQE"
     gene            complement(5976..7355)
                     /locus_tag="PXD94_001605"
     CDS             complement(5976..7355)
                     /locus_tag="PXD94_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL6286453.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSFAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAIAAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
     gene            complement(7477..8571)
                     /locus_tag="PXD94_001606"
     CDS             complement(7477..8571)
                     /locus_tag="PXD94_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6286454.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQKFVLYLGAVLS"
     gene            complement(8675..9127)
                     /locus_tag="PXD94_001607"
     CDS             complement(8675..9127)
                     /locus_tag="PXD94_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL6286455.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIKATVAK"
     gene            complement(9148..9900)
                     /locus_tag="PXD94_001608"
     CDS             complement(9148..9900)
                     /locus_tag="PXD94_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL6286456.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            complement(9911..11095)
                     /locus_tag="PXD94_001609"
     CDS             complement(9911..11095)
                     /locus_tag="PXD94_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL6286457.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDARKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFRVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
BASE COUNT         3234 a         2572 c         2348 g         3060 t
ORIGIN      
        1 tatggacttt ttaaccgaaa ttctcctttt cttaaaggat caagtgctga ataaagcacc
       61 cttcttacta ggtatcgtag cttgcttagg ttacattctt cttaaaaaag acacttctac
      121 gattattaaa ggcacaatta aaacaattgt cggttttatg attgtacaag ccggttcagg
      181 tttcttagta accaacttta agccggttat tgatggctta gccaaatatc accaacttac
      241 cggtgcagta attgaccctt acaccagtat gcaatcgacc attcaaacca tgggcgagaa
      301 ctacgcttgg gttggttatg ccgtaattgg ggcgctgttc ctcaatattc tattagtggt
      361 gttccgccgt ttaaccggta tccgtaccat tatgctcacc ggtcacatta tgttccaaca
      421 agcaggcttg gttgcggtgt tctatatgat catcggagcg tcaatgtggg agaccattat
      481 ctacacctca gtgttgatgg ctctttattg gggtatttcg tctaatatta tgtataaacc
      541 aacccaagcg gtaacaggcg gtgcaggctt ctcaattggt caccaacaac aaattgcttc
      601 ttggattgca gtcaaactcg caccaaaact tggcgataaa gacgatactg tagataaaat
      661 gaaattgcct aactggctca atattttcca cgacagtatt tctgcaaccg cattagtaat
      721 gaccatcttc tttggaatca tcttactttc attcggttta gataacctca aagaacttgc
      781 tggcaaaacc aaccagttta tgtacattct tgaaatgggc ttgaaatttg cggtggcaat
      841 tcaaatcatt gtaaccggcg tacgtatgtt tgtagctgag ctttcagaag ccttcaaagg
      901 tattgctgaa cgcttaattc caaactcagt attagcgatt gactgtgccg ctatctacgc
      961 tttctcgcca aatgcgatgg tattcggctt tatgtggggg gctatcggtc aatttgtggc
     1021 agtaggcggc ttgcttgcag taggctcacc ggtgcttatt attccgggct ttatcccgat
     1081 gttcttctct aacgcaacca ttggtgtgtt tgcaaaccaa tttggtggtt ggaaagcagt
     1141 aatgaaaatc tgttttgtga tgggtattat tgaagtgctt ggctcagctt gggttattca
     1201 aatgcttgct tcacaaggca caacctttaa cggttggatg ggtatggctg actgggcatt
     1261 gttcttccca ccaatcctac aaggtattat cagcattccg ggcttcttct ttgtaattgt
     1321 ggcagcagca atggtttata tgttcttcgc atctaaacaa cttcgtgccg aagaagcaga
     1381 agcggcagct cgtggcatta cacttgaaca attagacggc tacggcttag atgaagtaaa
     1441 agcagaacca aaagcggagg cagtagtaca acaagcggtc gaaaatgccg aaaattctgc
     1501 aaacccaatc cgcattctag cggtgtgtgg ctctggtcaa ggctcatcaa tgatgatgaa
     1561 aatgaagatc aaaggctatc tcgacaaacg tggtattccg aatattatgg attcctgtgc
     1621 ggtaactgat tacaaaggca aattaagcga ggttgatatt attgtctcca gcaaacattt
     1681 agccaacgaa atcgaagtac cggaaggcaa agcggtatta ggtgtacaaa atatgctaaa
     1741 tccaaactca tttggtgatg aattagttga gctaatcaat cagcataatg caaaataagt
     1801 aagtttaggg gtgaataatc acccctatct atgctaaaga ttgtaaaaac tcacttcatc
     1861 aaacgaggaa ttatccaatg aaaaaaatct tatccaccct gtttatgctc tccgcggtta
     1921 gcgttgccgt tgccaaagag gtaaacatta aactacttgg tacctcagac gttcacggac
     1981 gtgttgtgcc gtggagctac ggggcagacg tggaagacaa atccggctca ttcgcccaaa
     2041 ttgcaaccta tgtcaaagag gtgcgtgcca accacaaaaa tgtgttgctg tttgatattg
     2101 gcgatgcgat tcaagacaac caagtcgaag agttcgctaa aaccttagac aaagcgaaaa
     2161 acaatcccgt gccgaaagtg ctgaatgcaa tgaattacga catcttcatt ctcggcaacc
     2221 acgaatttaa cttcggaatg ccgaatttag atgcgatttt aaaagacatc aacgccaaaa
     2281 ttctcactgc caacttctat cacaaagacg gcaaacgcta cgttgagcca actaccatca
     2341 ttgaacgtga tggcgtaaaa cttgggatta tcggcttaac caccccaatg tcggctattt
     2401 ttgaaaaaga caccggtcat ttagacgcaa tgaagttcac ttcgccgatt gaagaggcga
     2461 aagcccaaat tgccgatctc aaagccaaag gcgtggacgc cattattgtg ctggcacata
     2521 tggggattga gaacgaaaac aacattcccg acaccggcgt ggcggattta gtgaatgcag
     2581 tggacggtat tgatgtaatt atcgccggcc atatgcataa agatgtgccg agtgaaacta
     2641 tcaaaaatac cctgattacc gaaccgcacc gctacggcac agtggtttcc gaagttgatc
     2701 ttgcctttga tgtgaatgac aaaggcgaag tgaaactact ttctaaaacc gccaaaaccg
     2761 taccggtgaa gaaactcgca agcgatccgg aaatcgaaaa aatttaccaa ccgtatcaca
     2821 atgaactgcg tgccttaaac aacgtcaaaa tcggtgaaac tgcacaagat ttaatcccac
     2881 aaggcaaaat cacggtgtat cgattgcttt ctcgcaagac accggaatgt cctcattgat
     2941 ccacgatgtg caacaacatt acagcggtgc agacgtagtg agttttgcat ttgactacga
     3001 aaccgtgcgt ttagacaaag gcgacatcaa gaaaaaagac atcatctaca actaccgcta
     3061 tgccggtggc gatgtgagca tgtatgagct aaccggcaaa cagcttaaac agtatatgga
     3121 atggtcagcg gattacttcg acaccattca agccggtgac aaagactacc gctacaacgc
     3181 tgtgcggggt aaatctaaat acgttacctt cgacctattc ggcggcgtaa gctacaacat
     3241 cgacctacgc aatccaagcg gtagcaaaat tgtcgatcta aaactggcaa acggcagtct
     3301 aatcaccgat gacggcaaat acaaagtggg aatgaactcc taccgctttg gtcagctcac
     3361 caaaaaaggc ggcatctggg aaggacagta aatccccgtg atttgggaat ccaaagtcgc
     3421 catgggggct gaaaaaggca caatccaaaa tatgctgatt gattacatca ccaacgtcaa
     3481 aggcggaaaa atcgaaggca aatcgcataa ccgctgggcg attatcggct taaattaacg
     3541 aaagggaaac actatgaact taaaacaatc tcttattgaa aataactcta ttttgctcaa
     3601 tcaatcagcg gcgaattggg aagacgcaat caaaatcggc actgatttat tagtcaaagc
     3661 cggtacgatt gaaccacgct attacgataa tattgtaagc aaaatcaaag agatggggcc
     3721 ttatattatt cttgcaccgg gcttggcaat gcctcacgcc cgtccggaag aaggtgtgat
     3781 taaaacctct ttcgctttag tgacttttga caccccgatt tattttgagg gcgaagatga
     3841 gccggttcac gttatgctaa cccttgcagg gagtgactct gatcagcata tgcaaggctt
     3901 ggtagaaatc acccaagtgc tagatgaccc agagagcgac acaggtgttg atttagataa
     3961 aatccgccgc tgtaaaacgg ctgaagaagt gtatgcagta atcgatcagg cattagccta
     4021 atattctaca agcggttcaa aaacatcaat attttgcaaa tagaggaata caattatgtc
     4081 caaaccatta ttacaaattg cccttgattc tctttcactg gaaaaagcgg tggcggatgc
     4141 aaaacaagca gaaaaccaag tcgaaattat tgaatgcggt actattcttg cttttgctga
     4201 gggtatgaaa gccgtttcca ccttgcgtgt attgcaccca aatcacatta ttgtgtgcga
     4261 cttaaaaacc accgatggcg gtgcaatttt agctaaaatg gcatttgaag ccggtgccga
     4321 ctggctcacg gtttcggctg cggctcaccc tgcaaccaaa gcggcttgca aaaaagtggc
     4381 ggacgaattt aacgctgctc acccggagct aaaagtgaaa aaagagattc aaattgaaat
     4441 ctacggcaac tggacggtgg aaaaagatgc caaagagtgg gtagaattag gggtaaccca
     4501 agcgatttac caccgctccc gtgatgcgga actagcaggt aaaggttgga caacggaaga
     4561 tgttgagtta atgaaacagc tttctgactt aggcattgaa ttatctatca ccggcggtat
     4621 cgtaccggaa gatattcacc tgttcaaaga gatcaaaaac gccaaagcct ttattgcagg
     4681 ccgtgcttta gtcggtgaaa aaggcaaaca aactgccgaa gccattcgtg tggagattga
     4741 taaatactgg aactaatccg gcattgaaac ccaataaaaa tggcgttctc tcaaccggaa
     4801 cgccattttt tatctcattt tttctattct tgcaaggccg atattttcca caatctcgct
     4861 acattttcgg cagttgaaat caggttctct ttgcctgatt ttaacaccgc ttctaaattg
     4921 ctgacctgtc gaataatcgg aaataccgca tcaatgccgt gttggtacac cacagggtaa
     4981 tcttctttca atgagccaac tatcgcaatc accggcttgc caaactgctt agcgactttc
     5041 gccaccccaa tcggggtttt accggcgata gattgagcgt ccattcgccc ttcccctgtc
     5101 accaccaaat ccgcatcggc aagtttctca gcaagatcgg ttgcctcaag aataatttgc
     5161 acgccggaac gtaatgttac gttaggcagt agcagtaaac caccgcccat tccaccggct
     5221 gcaccggcac cggctttctc ggcaatctca atgcctaatt gggattttac ggtttcggca
     5281 aaatgcaaca gggcgtggtc gagcatttta accatttccg gtgtagctcc tttctgtggc
     5341 ccaaaaattg ccgaagcccc tcgctcgcca cacagcggat tatcgacatc gcaagcaacc
     5401 tcaaattcca cttgctttaa gcgtgaatcc aaaccgctta aatcaatctg ctgaatttgg
     5461 cttaatgcct cgccaccaaa gccgatttcc cgctgttcgg catttagaaa tttcgctcct
     5521 aaggcttgta gcattccgac accgccatcg ttagtcgcac tgccgccaat gccgagaatg
     5581 attttttcca cccctaaatc taaggcggct ttaattaact gccccgtgcc gtagctggtg
     5641 gtaaccagcg gatttcgttg ctccattggc actaaatgca agccacttgc cgccgccatt
     5701 tcaataaagg cggttttttg atcccccgac aagccaaaaa agccctccac ctcatttgca
     5761 agcggtgcaa ttacgctgat tttttgcaaa ctgccgtgag tggcatcaac cagcgactgt
     5821 accgagcctt cgccaccgtc tgccatcggt acgagctgat actccgcctc agggaaaaca
     5881 cgggcaaaac cctgttgaat tgcttgagcc acttctaatg cggtcaaact ctctttaaat
     5941 gaatccggtg caatcacaat tttcatcgtc aatccttaga gtaaaatcaa tgaaagcaga
     6001 taaaccgtga gcatacccac cacacccata atgaaagtta gggtagtttg cgtacgatag
     6061 ccttgttgcg gagtcagttt agtaaagttt gttaccaccc agtagtagct gtcattggcg
     6121 tgggaaacgc acattgagcc agccgcaatc gccattacgg ttaaggctgc cgccatttca
     6181 ctggttagcc ctaaggcgtt cattaatgag tccggtgcgt tgaacattcc cataatggag
     6241 gcggtggtga taattgccac cgttgagctg ccttgtgcgg tttttaaaat tgccgaaatc
     6301 aggaacggga agaaaatccc tgctgtactg ataatatgag cgttttgctt gatgtaatcc
     6361 acaaagccgg cttgagtaat cacgttacct agcacaccac cggcggcggt aataaagaga
     6421 attgggccga ctaatttcag ggtatcattg gttaagctgt caaattccgc cattttaccg
     6481 cttgaaacca gtaggaaaat agcgaagatc accccaattg ctaacgcaat aatcgggttg
     6541 ccgacaaatt gtaggaatac gccgaaatcg ccacttacac cggcaatttt ggcaatcgag
     6601 ccgatagcca ttaagataat cggcataaaa atcggggcaa agcttaaaaa cccacttggt
     6661 aatttgccgt attgtttgat cagatcttca tagctttggc tgattacttg atccgcttga
     6721 gcttcttcca tcacgctgac ttgtttgcca acatatttgg caaagaagta actggcaatc
     6781 aacacaggaa tcgaaaccac aaagcccatt ccgatcacaa gcaggagatt atggcttaaa
     6841 ccaacagcac cggctgctgc aatcgggccc ggtgtcggag gaataaagac gtgggaggca
     6901 tataaaccgg cacttaacgc caccgccatt cccaccggat tggcaagaat ttttttgcga
     6961 atagcttcac gaatcgggtt taacaccaca aagccgctat cacagaacac tgggatacca
     7021 accacccagc ccataatcaa cattgccagt tcagggcgtt tttgccccac cacttttacc
     7081 accatatcgg ctaatttgag ggctgcaccg gttttttcta aaatcgtgcc gataatcgcc
     7141 ccaaaaataa tcacgatacc gatacttttg aaggtattac taaaaccttc gcccacaata
     7201 gttggaattt tggcaagcgg taaacctgcc actaatgcaa gggctatcga aatcgacatt
     7261 aaggctaaga acgggtgaac tttccacttc gcaatcatat aaatcattat gatgatagcg
     7321 accagaaaga tcagaataag tgcaatacct gacataataa gtctcctcat tgtagaaaaa
     7381 tgaacttatt attcggtatt tattgctaaa aagatttaga taaatttgca aaaaatttgt
     7441 taaaaacgac cgcttgtttg tatattttta caatttttac gacagaaccg ctccaagata
     7501 cagcacaaat ttttgctcta tgttattgaa agataaacca gttatctgct caatcttctc
     7561 taaacggtaa cgtagcgtat ttgggtgaat aaacaatttt ttggcagcgt gatccagatc
     7621 acaatttgcg aaaaaatagt aacgcagact tttcagtaac acctgttttt tatcggaaac
     7681 cagtaaagct tggaacggcg agagcaaggt tttcgcttcc caagagtggc tgaaactgtg
     7741 caagagggcc ggcagtttaa aatcttcaaa atgaataatc tgcttacggc tttggatctg
     7801 ctcggcataa ttgagggtat ggcaggcggt ttggtaggaa atatgggttt gcgataaatt
     7861 ttcgaccgcc agcccaacca ccgttttgaa ggaaatatcc ggatcgttaa gtttgtttag
     7921 tgcattgcgg attaacgatt tttcgttgga taaatgattc agcaaggcaa ttttatctaa
     7981 cccaatcacg gcggtgagta ggctcgggaa ataatgttcg aaaaaatcaa tcaggcgttg
     8041 gagtttttcc gcagtagagc cgtgaatttt aatcaccatc accgacagcg gttttgcaaa
     8101 atcttgcccg aaaaagaccg cttgttgctg gatttctgct gtgcttaggc tacctttgag
     8161 caaggcccgc acaaactctt cccgatagcg gcgttgccaa cgttcctgtt ccaactcaaa
     8221 cgcttgctgc ataatcagct ccgccgccat tttcaccaat tgagcatatt gccgaacttg
     8281 ttgtggctgc cctgaaatcc ccactacacc aatggttgag cctaaatagt gaagcggtaa
     8341 gttaatgccc gctttcactt catcgcacca ctgttttgca agattttcat caatttcgac
     8401 cgcttgatcg ttgcgtaacg caatcaccgc tcctgtgtgt cgttgcccga tacgaccgac
     8461 atcgccggaa gcaataatca ccccttcact atccatcaca ttgacggagt tgggaataat
     8521 ctgcatcgtg cgcttgacga tgcggtcggc aatcgcttgg ttgagtttca ttatatgatt
     8581 cctactcatt cccaatacta tgaaaaatcc ctctttcgag ggatttgcaa attatctacg
     8641 cttccacttc ctgagaatag atcatagcgt gaagttattt tgccacagtc gctttaatta
     8701 atttttccgc tttggcaagg ttgttttcta catcagcata atcggtatta gcgtgagcca
     8761 ccacttgatc ggcacgattt aacattactt cgaccttgcc cttgttcggt tcggtaatta
     8821 acacttttaa cggtaattgc aacgctaaca tcggatcttt caccattaat ggcgtaccgg
     8881 ctttcggtgt gccgtaaacc aatacggttg ccggttgcat ttctaagccg gcttgttttg
     8941 ccgccgcttg gtgatcgatt tcggcaaaaa tcgtcattcc tttttctttt aaggtgttgg
     9001 ttaaagttgt gactgtagtt tggaaatcaa atttactttc aaataccacc ggtttgacat
     9061 cggcaatatc tgccattgcc acgctgctca ttgctaatgc agaaactgcg actgctttta
     9121 agttcatttc ttttctcctc aaatagatta aataatccct tgttcttcca acttgcctaa
     9181 gaaataaggc atctgtttac gccaagtcgg ccagtcatgg tcaatatcaa agccccagta
     9241 gtcaaaccaa gcatcgatcc cttttgcgtt aaaagcctct tgtaatcggc gggtgtcttc
     9301 aatatgctgt tcttcccacg caccttgtcc gactgcaatc acatagtgat tttggcggta
     9361 gcggtctaag aaccaatcgt cattttgacc ccatacataa tcaatcggcg agttaaaata
     9421 aaccgcttta tcgccataaa actcaccact gaaaaagcga gcgtcataaa caccgcttaa
     9481 ggcgatggca atatcaaaca gatccggatg acgtaaggca aagtttaccg tatggaaagc
     9541 ccccatacta caaccggtgg caatcatcgt gccgttccag tgcgattcat ggcggattaa
     9601 cggcactagc tcgtgaataa tataacggtc gtaagcatta tgagctaatg ccatatcgtg
     9661 tccggattta tgtttagata accaagattc cgcatcgtaa gaatcaggcg tgtaaaattt
     9721 gattaagcca cgttcaataa aggaacggca agcctcaatc attccaaaat taccatactc
     9781 gttttcattg ccgccggagg aagggaaaac aataaccggt ttacctgcgt gtccgtaaac
     9841 gttgaacgac atttcacgac ctaattctcc actccagtgg cttctttttt caaaatgcat
     9901 acttcacctc ttactgttta gcttgtgcaa attgtgcaat ttcacgcatt tgctccattg
     9961 tttcggtacg ggctaaaatg ccttgttcgc ccatcacttt ggcaaatacg cccggtacgc
    10021 tttgcacgct gataatattg ccggcaaatt tctcacaaac agcttcaatc gaattcacat
    10081 aattttggtt tgccttgcgg gaaatataaa ccacgttcca agggtgctga atatcggcat
    10141 agaatttgtt ttctttcaca atattggcaa attcacggaa cacatcgaaa tcgttggcgt
    10201 agttccacat atcaatagtt aagccacccg gcggacggca gttgatttcg agtggtaaca
    10261 actcttcttt tttgctccct ttaacacgga agaactcaaa atggaaaaaa cgctctttta
    10321 cgttaaaggc tttcacacat tgtttgccca tttccactaa ttttggcgaa atttcacatg
    10381 ggacatagta gaacatatcg ccatcttttg aaacggtatc taataccgct tctgaatatt
    10441 caaggctgga gtagaaaacg atattgccct ctttatcggt taaaccgtca aaggtaataa
    10501 tatcgccgtc aataaactct tccataatat attcaaccgt tggcgtttta taaccgaaga
    10561 aagcttctaa ctcttccgca gttttgattt tgtaagtatc gcttgcccca acccctgaat
    10621 ttggcttgat aatcaccggg aatttaagct gttttgctaa tttgcgggca tcttcatcat
    10681 ctttaaatac tcgcccttgt gccactttca agccggtttt gcggaatacc tctttcattt
    10741 gggctttggt tttaattgag agcatatcat cgctcttata accaaacaca ttgaaatcgg
    10801 tacgcaattt cgcatctaac tccagccaat attcattgtg cgattcaatg cggtcaattc
    10861 gcccgtattt atgggcaaag tacgcgaccg cacgataaac ttgttcgtaa tcttccatat
    10921 tatctacacg ataatattca gtcagggaat gacgcagatt ttcgctcaat gattcataag
    10981 gggcatctgc aattcccaat gtattaaaac cggcttcacg caaacgaatg gcaaaggttt
    11041 caaagttcgt tgggaagtgc ggggaaatca tcacaaaatt aagtggtcta gacatagttc
    11101 ttcctttata aagtttgcat attattaaaa tgattagaat agattttagg ctaaatttat
    11161 taattttcaa aatgtattca cacaagcaat aatttttaca ataaattaac tttt
//
LOCUS       DANLXQ010000082        11063 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.082, whole genome shotgun sequence.
ACCESSION   DANLXQ010000082 DANLXQ010000000
VERSION     DANLXQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11063
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.082"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..242)
                     /locus_tag="PXD94_001610"
     CDS             complement(<1..242)
                     /locus_tag="PXD94_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286458.1"
                     /translation="MITSNLERSFHGLNIAQHNYKIFLDESGGNIGKLKHTPNDKKGS
                     NFNRTISSHIGVPPVFVLGGIAQISNANLIAPDWRKL"
     gene            complement(269..943)
                     /gene="cas4"
                     /locus_tag="PXD94_001611"
     CDS             complement(269..943)
                     /gene="cas4"
                     /locus_tag="PXD94_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL6286459.1"
                     /translation="MGDLQNSAEILPLVQPKFGDDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQALHERVDSGEPEVRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKLDPIDEIQLCAQALCLEEMTEQTINEGALWYMQTRHRLPIIF
                     STELRTKTLATIAEVRSLLDSGTTPPPEYSKRCKACSLIDLCQPKLLERDRSKGYVVG
                     VFGSLL"
     gene            complement(943..1806)
                     /gene="cas7c"
                     /locus_tag="PXD94_001612"
     CDS             complement(943..1806)
                     /gene="cas7c"
                     /locus_tag="PXD94_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL6286460.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYCISVQTE
                     GLTGVTVEELL"
     gene            complement(1816..3597)
                     /gene="cas8c"
                     /locus_tag="PXD94_001613"
     CDS             complement(1816..3597)
                     /gene="cas8c"
                     /locus_tag="PXD94_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL6286461.1"
                     /translation="MITALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDSK
                     GNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATAK
                     DQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDAN
                     VVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGVF
                     GGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGDA
                     STVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPELS
                     ENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCPWKTPPSIWRLL
                     LQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMKA
                     VLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQSQALGELNAGIADRYYG
                     SASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSIDE
                     QGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(3619..4296)
                     /gene="cas5c"
                     /locus_tag="PXD94_001614"
     CDS             complement(3619..4296)
                     /gene="cas5c"
                     /locus_tag="PXD94_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL6286462.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(4538..6289)
                     /locus_tag="PXD94_001615"
                     /pseudo
     CDS             complement(4538..6289)
                     /locus_tag="PXD94_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(6279..6740)
                     /locus_tag="PXD94_001616"
     CDS             complement(6279..6740)
                     /locus_tag="PXD94_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286463.1"
                     /translation="MFKPHGSLDWFMAGDVPMTSCIGKVEEPLIITPGVGKYKKGYGQ
                     PFDAHREKANEAIDSASSILCIGYGFNDDHLQTHLTGKIKSGVKTLLLTRGLSENARN
                     VVKEAPNCKALIFDGDKAGTRVVSKQEETFIPNVHWWDVEFFVKEILENGK"
     gene            complement(6899..8035)
                     /locus_tag="PXD94_001617"
     CDS             complement(6899..8035)
                     /locus_tag="PXD94_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6286464.1"
                     /translation="MLLRKAFKFEIRPNGEQVRKMKQFCGCSRFVFNKALAWQNEQYE
                     QDNHHKFSYTKLANLLPQWKKELVWLKDCHSQVLQQSLKDLESAFKNFFQKRADLPKF
                     KKKGVKESFRFPQGCKIEQKNDRLYLPKIGWVRYRNSREIVGEVKNVTVSMKCGRFFV
                     SIQTEFKQEIPTHKGGEIGIDMGIARFATLSNGEFFEPLNAFKTHKGKLAKLQKQLKN
                     KVKFSKNWQKLKDKIGKLHHKIANCRKDFLHKISTQISKNHAMIYVEDLQVANMSKSA
                     KGTAEVHGKNVKQKSGLNRVILDQSWFEFRRQLDYKTQWLGGFLLAVPPQNTSRTCPC
                     CNYTAKENRQTQENFECVECGYTENADVVGAMNVLTRGQAIVQA"
     gene            8082..8498
                     /gene="tnpA"
                     /locus_tag="PXD94_001618"
     CDS             8082..8498
                     /gene="tnpA"
                     /locus_tag="PXD94_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL6286465.1"
                     /translation="MKKETEIRRGRHVVFNLHVHLVFVTKYRREVFTQAILNDLHSIF
                     ATICADFEATLVEFDGEDDHVHLLVEYPPKVAISHLVNSLKGVSSRMIRKKNYPSIRK
                     KLWGNQLWSPSYFAGSCGGAPISIIRQYIEQQQTPD"
     gene            complement(8704..9696)
                     /gene="mglB"
                     /locus_tag="PXD94_001619"
     CDS             complement(8704..9696)
                     /gene="mglB"
                     /locus_tag="PXD94_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL6286466.1"
                     /translation="MKRTILSAFALSVICGVNLSYAQSESRVGVTIYKYDDHFMTQMR
                     KDMQNEAKGFAGLKWFMNDAQNSQVKQLAQIETLLQHKMRVLAVNIVNANESKTIVEK
                     AKAYNVPVVFFNRTPSKQALESYEKAYFVSADPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
     gene            complement(9781..10194)
                     /locus_tag="PXD94_001620"
     CDS             complement(9781..10194)
                     /locus_tag="PXD94_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HDL6286467.1"
                     /translation="MTAWERVFHALLFECFAIIFTVILTSWLTSHRMVDLTAVIVMIS
                     VIAMVWNVIFNWGFDKVFTGERVQRGLGIRILHSFLFEGGLLVFTIPLVMYMLDIGWW
                     QAFVMDIGMTLFVLVYSVVFNWVYDHLRLRWVKSG"
     gene            10314..10601
                     /locus_tag="PXD94_001621"
     CDS             10314..10601
                     /locus_tag="PXD94_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL6286468.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVATIQRTYESENEAKAAL
                     EKFTQKARDVESEPCEISSEITPIESGFELVAHFKFSCQAEVVIFQLGTRF"
     gene            complement(10647..>11063)
                     /locus_tag="PXD94_001622"
     CDS             complement(10647..>11063)
                     /locus_tag="PXD94_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL6286469.1"
                     /translation="YLDLDLSPYFNSGVLLINNILWKKYSVCDELVTINEKIVNLTYG
                     DQDALNIYFKGGWGALPIEYNYQVDAILELVLRREQEELARKNGYLDVIPKIIHYTSK
                     FKPWKKELHTMQISTRKKYWFYYHLEWNDILEQHQK"
BASE COUNT         3131 a         2501 c         2020 g         3411 t
ORIGIN      
        1 agttttctcc aatcaggagc aattaaatta gcattactaa tttgagcaat ccctcctaat
       61 acaaatacag gaggaacccc aatgtgggaa gaaatggttc tattaaaatt actacctttt
      121 ttatcattag gcgtatgttt taacttccca atatttccac cactctcatc aagaaatatt
      181 ttgtagttat gctgtgctat atttaatccg tgaaatgatc tttctaagtt tgaagttatc
      241 acaacaagtc cctccccaaa gtaggtaatt ataataaaga tccaaacact cccaccacat
      301 accccttcga tctatccctt tccaacaact ttggttggca gaggtcgata agcgagcagg
      361 ctttgcagcg tttgctgtat tctggaggag gtgtggttcc gctatctaag agcgaccgca
      421 cttcggcaat ggtggcgagg gttttggtgc gtagttcggt ggaaaaaatg atcggcaaac
      481 ggtggcgagt ttgcatatac cacaacgccc cttcgttaat cgtctgctct gtcatttctt
      541 ccaaacataa ggcttgggca cagagttgga tttcatcaat cggatcaagt ttgggcttgc
      601 cacgtttgta ttccactggt tttagctcgc ctgtttttag gtctcgctca accaaatcca
      661 aaatgccgct tatgcctaat ttttcggctg caacgtgaac cgttcgctca aagcgaatcc
      721 ctttgcgaac ttctggctca ccactatcta cccgctcgtg caacgcttga ccttgtgcgg
      781 tgagatagtt ttctgcccac acctgttcgt tgtggatcag cgcacattga cgtgggcaga
      841 aggcatagtg ttgcaaagcg gaaagcgaaa ttgaccgctt gtcatcgcca aattttggct
      901 gaacaagcgg taaaatttcc gcagaatttt gcaaatcgcc cattacagta attcttcaac
      961 ggttacaccc gttaagcctt cggtttgtac gctgatacag taatcgtcat agtctttcgc
     1021 tggcgtgtct ttttcaccgt tgattcgctc aacttttacc gagtcaaaca gtttgtgtgc
     1081 aggttggttg cctaattcgc tgtcgtgttt aaatacgatc agtttacgag ctgccatttc
     1141 gcctcgtgcg gcagaacggt cgtgttcaaa cataagttgc aaggcttgcc aaagtttttg
     1201 tacatcttca tcagaaaagc ccgttttttc agcgagttta gctgaaataa agccgtgaac
     1261 acgataaagt gcgtaaggca cgatatattt acgccccata gtccgttctt tttcaagatc
     1321 tttttcgttg gtcactgcca ttcgtgtgat cgatacttct agcggaataa tcggatcgat
     1381 agattgagcg aacgccaatt gcactgggcc tcgcacctgt ccgctgttta cttcggtagt
     1441 cattaccgcc ccaaaggtgc gaatatcgaa aaagtttttg cacatccaag cggtaatttc
     1501 tcgggcttta gcttcctctt ttggtagttt tttcgcttcc gattcaatcc ctaatgcttc
     1561 ataagcacgt ttgttttgta aattcagaac acttttttct ttaacataaa tttcgtaacc
     1621 cgcctcgttt tcatagctca tttcaataaa attgcggatt ttgcgtttta ggcaaacatc
     1681 ggtgactaaa cctttactgg tttcaggatc aaggcgtggc atatttcctg catctggatc
     1741 accatttggg ttaccgttgg ttacatcaaa gaaaaaaaca aattcatagc ggttttgaat
     1801 agacatagtg tttccttagt tttcaataga ctcagtggtt tcttttttag cgaaatagct
     1861 ttgtttttgg tggtaataac caatcgcaaa acgcccttgt tcatcgatac ttaaatggcg
     1921 agggaaattt tccggcaagc tggcaataat ctcacctaaa tccttatcca gattaaccgc
     1981 cattccgccc ttatcttttc tcaagcgaga taaatggtgt tttgcaccgc ttaataagcg
     2041 agggaatacc gaaaacggca cggcagaagc cgaaccataa tagcggtcgg caatgccggc
     2101 atttaattcg cctaatgctt ggctttggat tctttctaat actgcaaata acctgccgag
     2161 caagtaagct tggttatcgc tttctttatt taagctcata ggaactcctt ctttaataaa
     2221 accttttcta aattttcgtt gtaagactgc tttcatcaac gctactcgca aaccgttaat
     2281 atcgccgtct gctcggattc gagtcactaa ctgtgaaagt aaactcatcg gatataaatg
     2341 accgctaata actgcgcgag ccatttcacc cgccaagact ggagaaatat tctcactttt
     2401 acctaatgaa gcggtttgta ataatagtcg ccaaatggaa ggtggtgttt tccatgggca
     2461 aggctcaaga gcgagatctt gccaatgctc gctgaggttt tttgccagtt gcccaaaggt
     2521 ggtatccagc caaaaacgta ccgaaatgcg agctgcattt ggagcaagcc ccaacacata
     2581 aaaacgggtg ttttcagata attctgggga aatttctgca agcggtcgtc ctttgctgat
     2641 tttttgcaat tcttcaaaga ttttttggtt ttcttgctca tcatctggag gcgtcattaa
     2701 agcagcaaaa aagccttcag cagcttcagc ttgggtggta ttgtctgctt ctgcccaaaa
     2761 gacagtgctg gcatcaccaa tagttaaacg atgtttctcg gtgcgtaaca aataatttaa
     2821 agcagtagtg taggcaaagg cggcttgttc agaaacaggg gcattactac cttgttcctt
     2881 gccaaatgaa gtgaaggatt ctttattgaa agagatgatt gaaccaccgg aactttgccc
     2941 accaaatacc cctttaatag gaggatgagt tcgttgaatt ggcgataatt tgcctgaaat
     3001 taagcataaa cctttttcag cattatcact ttgatttatc aacaaacttg tccaaagggt
     3061 ttgtgcagct tctcgctgat gaatataacc gcttgtgccg actaatttaa acacgacatt
     3121 cgcatcaaga atttctcttg ggcagattga ttcggcaaaa tgtgtgggtt gccattgttg
     3181 caggaaattt cttatcgcca ttaaaccttc atcattggaa tctgcaagca aatccaaatg
     3241 gtactgttta aatgcctcaa aggttttctc ggcgaaattg atgggttgat ctttggcggt
     3301 ggctttatca ggattacttt caatgcctag cacataagcg gttttatccc acagaaaatt
     3361 gggcttaatg ccagacgtgc gtttttcagg gcgaggtacg ctcatcaatt tcggttttgg
     3421 tttcttatct tctgttaaat ttgagatcac attgaccaaa tttccctttg aatctaattc
     3481 tagtacccaa ccgatttttt cttcactgaa accataagaa ggcactttag cttcgcctgt
     3541 taagcgatct tcttgtgctg caaggcggta ataatagcga gttaaggcgg taataatagc
     3601 gagttaaggc ggagagaatc atgatttgac ctcctctgca taaaatggtg gcacatcaat
     3661 cacgccattt ttcatttccg cacggaagaa acgaggttga ttaccgtgct caaaatcaat
     3721 gtcgtgcaac atccagccta aatcccgatc tcgctcattt tctgccaaaa aacactcagg
     3781 taacggttca tcgtcatcaa tcaaggcaaa atttgcggca aattcacgca cgcccataca
     3841 aggctgctgg aaacattgcc cctttttcgc acggcgttta aacatttcaa tatgcttggt
     3901 gacggtttcc gcctcgcctg ccattttggt cagcactgcg tgagcttcga tcacataacc
     3961 gacatttttc agcacagtag cagcacgttg ttggcggtcg tcttcaatca cagtatataa
     4021 atccgccact gttttacgct tcattgcacc actgatttta ctggcagaaa ttttactgcc
     4081 taactcatta cggcgaacag actcaaattg aatcggttgt aatacataaa ttttatcaat
     4141 cacccagcga attgccggct tccaatgcac cgcagccaaa atcccccgtg ccgcagacgg
     4201 cgtaatcaca tcataagaca cccgttctac cttcatttca ggacgggtaa aacaagcata
     4261 atcaccccaa atatggagcc tgattttgtt tggcataata gaatgctcct tttaattatg
     4321 taacacacac ctccccgaag ggaggtgaaa tttctttagc ttatattata actgctttgg
     4381 tttcaaccca cgcactcagt aaaatgcgaa aaattttatg gcttattata ttaagcggtc
     4441 ggatttactt agaaagttgc aaagttattt gagtatttaa ccgcttgtat ttcaaaaata
     4501 gtcagaaaaa taacagcatt ctaggtacaa tatttttcta agaacgaact tgtctccgca
     4561 atgcctctac tgcgtttgac acagcatttg gtttgggctc ctggtttgcc caatcagacc
     4621 agaattggcg tgtagcactt tctggggcta tatgcggttt agcaagtttg attcgtgtag
     4681 gtaatagaat cgaatcgcct agcaataacg cttcgcctaa atcaagtaac ggcaatatat
     4741 cagtaattcc aaccatagat tccggcaata aacgctttac aacactttga tcttgaccat
     4801 ttgttaatcg taaaaccaag aaattattac attgactcaa aattgttcgg cttacatctg
     4861 aaggacgttg gctaacgacc aacaaagaca cgccatattt tctaccttct ttcgcaatcc
     4921 gctcaaaggc ttccaatgcg cgtttttccg ctgcttccgc tgaatctcga acagggagat
     4981 ataaatgtgc ttcatcgcaa ataatcgtta gtggatggcg ggaatcttta tccatccaaa
     5041 attgaatgtt gtataaaagg cgggcaagaa ctcccgtaac aatcggtaaa acatccgacg
     5101 ggacttccga aaaatcaata atttttatcc ctttttcgct ctcagaagca gaaaacagtt
     5161 ttgacaacat attttccagc cacccatagc tgttgcactc tactggcggt tggaacataa
     5221 aaccgcaacg tctgtcatct attttggctt tcatccttcc cataaaccta gacaacttac
     5281 tataccaagg accttgcttt gttcctgaac ctccctcttt aggtaccatc tcagaattat
     5341 ctttatctaa acgagctaac aataattcaa tagaatacgg aattggcgaa tcaacagtaa
     5401 acgtttccaa aacatcattt ctttcttcct tattcaatgt ttgctctttt aatgcgcgaa
     5461 catgctcagt aaaacgcgcc gcctgattcg gagcattatc atcactgcga tctaaaatta
     5521 aagccagcat ttattcccga ttgagcagcc aatagggaat aaataaactg cgttcatctg
     5581 aaccatccaa atctcccggt ccagcaatac ggtattgctg agcgatagcg tttttacctt
     5641 cgcatagagt gctatattca ccgtgcatat cgaatacaat aagattagca gcttttaaag
     5701 ctgctgcacg ttccaatatt aatgcaactg accagctttt accggaacca gtactaccca
     5761 aaatagcagc gtgccgttga aagaatttat ctccgtttgc tatagctaca gccgtttcat
     5821 cagccataaa atgacctaaa gtcagccgtt cattttcttt tacgttagcg gacaaaagat
     5881 tcataaataa ttgcagattg ctgccactaa tatgaaagca atctctatca atttgcggaa
     5941 aacaatccgc tccccgctta aaagtgttgc tcaaactacc atcaacagct ttataagtac
     6001 cgattaatac tgccttgata aagtcatcct gactttcacc cagaggaatt tcaccctcat
     6061 tagatatttc ttctagcagc aatttttcac tgatagaacg cgttacccgt tcgataatgc
     6121 cgataaggta ctcactagcc gttctgcctt gaatcgcaat cagattaccc acagcaatac
     6181 tagtcatttg ttgatgctct tcaacagaaa tcaatacacg gctggtatct actgttacaa
     6241 ccctgcccaa acgggcattg tctttaaaat tcaatgtttc atttgccatt ctctaaaatc
     6301 tcttttacaa aaaactcaac gtcccaccaa tgaacattag gaataaaagt ttcttcttgc
     6361 ttgctaacca cccttgttcc agccttatct ccatcaaaaa tcagagcctt acaattcgga
     6421 gcttccttca ccacgtttct ggcattttct gaaagtcctc ttgtcagaag tagtgttttc
     6481 acaccacttt taattttgcc tgtcaaatgt gtttgaaggt ggtcatcatt aaaaccatag
     6541 ccgatacata aaattgaaga agcagaatca atagcttcat ttgctttttc tcgatgagca
     6601 tcaaatggtt gtccatagcc ttttttatac ttcccaacac caggcgtaat aattaaaggc
     6661 tcttccactt tacctataca acttgtcatt ggaacgtctc cagccataaa ccaatctaaa
     6721 ctaccatgcg gtttaaagat tttgatatga tctcgttgac atcctcccct gcctaaaggc
     6781 aggggattcc tactagctcc cacagcaagc tgtgggctac tctcggtggg tttctactgc
     6841 tgatctctat tgagattcac ttcatagacg cttcgggtat gtcctaccct gatatttatt
     6901 atgcttggac tattgcttgc ccacgtgtaa gaacattcat tgctcccact acatcggcat
     6961 tttccgtata gccacattct acgcattcaa aattttcctg tgtttggcga ttttcttttg
     7021 ctgtatagtt gcaacaaggg caagtacgac tggtattttg tggaggaact gctagcaaaa
     7081 atccgcctaa ccattgcgtt ttgtagtcca gttgtcgtct aaattcaaac caagactggt
     7141 ctaatatcac tcggtttaag cctgatttct gtttcacatt tttgccgtgt acttccgctg
     7201 tgcctttggc tgatttggac atattcgcca cttgcaaatc ttcaacatag atcatcgcgt
     7261 ggtttttgct gatttgcgtt gagattttat gcaggaaatc ttttcggcag ttggcgattt
     7321 tgtgatgtaa cttgccgatt ttatctttca gtttttgcca gtttttactg aacttcactt
     7381 tattttttag ctgtttttgc agttttgcca attttccttt gtgggtttta aacgcattta
     7441 acggctcaaa aaattctcca ttggaaaggg ttgcaaaacg agcaatgccc atatcaatgc
     7501 cgatttctcc gcctttatgc gtgggaattt cctgtttaaa ttccgtttga atgctcacaa
     7561 aaaagcgtcc gcatttcatt gaaacggtga cattttttac ttccccaaca atttcacgac
     7621 tgttgcgata acgcacccaa ccgattttcg gcagatacaa gcggtcgttt ttctgctcga
     7681 ttttgcaacc ttgcggaaaa cggaaactct cttttacgcc ttttttcttg aatttgggca
     7741 aatccgcacg cttttgaaag aagtttttaa acgcactttc aaggtctttt aacgactgtt
     7801 gaagcacttg tgaatggcaa tctttaagcc ataccaattc ttttttccat tgcggaagca
     7861 agttggcgag cttggtataa ctgaatttgt gatggttatc ttgttcgtac tgctcatttt
     7921 gccacgccaa tgccttgtta aacacaaagc gagaacagcc gcaaaactgc ttcattttgc
     7981 gaacttgttc cccatttggt ctgatttcaa atttaaaggc tttacgtagt aacattgcac
     8041 gaattagaat acaaaaattg tgcttattct acacttggcc tatgaaaaaa gaaacagaga
     8101 ttagacgtgg tcgccacgtt gtttttaact tacacgttca tttagttttc gtgactaaat
     8161 atcgcaggga agtttttact caagcaattt tgaacgactt acactcaatt tttgcaacga
     8221 tttgtgctga ttttgaagcc acattagtgg aatttgatgg tgaagatgat cacgttcatc
     8281 tacttgtgga atatccgcct aaagtcgcta tttctcatct tgtaaacagt ttaaaaggcg
     8341 tttcgagccg aatgattaga aagaaaaact atccgagcat tcgcaaaaaa ttatggggaa
     8401 atcaactttg gtcaccgtct tactttgctg gtagttgtgg tggtgcacca atatcgatta
     8461 ttcgccaata tatcgagcaa caacaaacac ctgattagtt agaaatttga aacggctttg
     8521 ccgtttatac cttatatcca cgccctaaag gacgtggttt tacggcacga ttagataaaa
     8581 tttgaatatt cgctctattc aaatctgttg gaagaaaaga gaaaggattt gcaagatttt
     8641 actgaaaaat gaccgcttgt tatcatcaat aaggggcgta tttataacgc cccgctcctc
     8701 catttaaggt aaaaattgcc ctaaattttc cttatcaata ccaatatgtg gaagataaat
     8761 cgttttagag gaatggcttt cccactgcgt atctttcagc gcaggcttac cttgtgccaa
     8821 attagatacc aatttcacca ccgctttcgc ttgtgcagag ctatcattta ataccgtgcc
     8881 ggctatttcc ccttttttca ttaatgctaa cgtctcaggc aaggcatcaa taccaaagat
     8941 cggcaatctt ttctcgtgtg catttaacgc atctaaagca ccaattgcca tttcatcatt
     9001 gtttgaaatc acaacttcaa tctcagctgc acggttttcc accagccact cattcatttt
     9061 attgcgagcc aatgttctac gccatctcgc cgtgtcactg taaatttctt ctgcctgaac
     9121 accttgttga ttaagcgatt caaccacagc ttgagaacgc tcagcgctca catcactcgg
     9181 ctcgccctta agcaatacaa attgcaattt cccgtcttta tttagatcga aatcggggtt
     9241 tgatttccac actttcgcaa ttaattcccc ttggattcta ccggcctctt taggatcagc
     9301 gctcacaaag tacgcctttt cgtaactttc taacgcttgt ttactcggtg tgcggttaaa
     9361 aaagacaacc ggcacattat aagccttcgc tttttccaca atagttttcg actcatttgc
     9421 attcacaata ttcaccgcca acacacgcat tttatgctga agtaaggttt caatttgcgc
     9481 taattgcttc acttgggaat tttgtgcatc attcataaac cacttcaacc cagcgaagcc
     9541 tttcgcctcg ttttgcatat ccttacgcat ttgagtcata aaatggtcat catatttgta
     9601 aatcgttacc cccactctgc tttcagattg tgcgtaactt agattcacac cgcaaataac
     9661 agaaagtgca aaagcactca agattgtacg cttcatagaa tgtccctctt gctatgccaa
     9721 atatgaacca attcaagcat agcgaatttt gtgtaaggaa accgtgatct agttcacaat
     9781 ttagccagat ttaacccacc tcaggcgtaa atgatcgtac acccaattaa aaactaccga
     9841 ataaactaat acaaataagg tcattccaat atccatcaca aacgcctgcc accagccgat
     9901 atcgagcata tacattacca atgggatagt aaaaaccagc aaaccgccct caaaaaggaa
     9961 tgaatgcaaa atacgaatgc ctaaaccacg ttgcactcgc tcaccggtaa acaccttatc
    10021 aaaaccccag ttaaaaataa cattccacac cattgcaatc accgaaatca tcacaatcac
    10081 tgcggttaaa tcaaccatac gatgagaagt cagccaagaa gttaagataa ccgtaaaaat
    10141 aatagcgaaa cattcaaata acagcgcgtg aaacacacgc tcccaagcgg tcatttttcc
    10201 tccaaacttt gcaaaatacc aactaaatca aataatcgtt cactgatgat tgcaggttac
    10261 gcaatttagt tttaaagtac ctacattatt catcaattta gaaggattag ccaatgaaaa
    10321 acgataaacc gattgaatgc gaaggctgta acacatttga tgtaggttct attttagata
    10381 acagcgaaac agtcgctact attcagcgca cttatgaaag cgaaaatgaa gctaaagcgg
    10441 cacttgaaaa attcactcaa aaagcccgtg atgtcgaatc tgaaccctgt gaaatcagca
    10501 gtgaaatcac cccgattgaa agcggttttg aactggttgc tcacttcaaa tttagctgcc
    10561 aagcagaagt ggttattttt caattaggaa cacgtttcta atacaaaaag ctgattcaaa
    10621 cacttgaatc agcttttgta tgatagctat ttttgatgtt gttccaaaat atcgttccac
    10681 tctaaatgat agtaaaacca atatttttta cgcgtagata tttgcattgt atgcagttct
    10741 tttttccaag gtttgaattt actcgtatag tggataattt tcggaatcac atctaaatag
    10801 ccattttttc tcgcaagttc ctcttgttct cttcttaata ctagttcaag aattgcatca
    10861 acttgataat tatactctat cggcaatgct ccccaaccgc ctttaaagta aatattgaga
    10921 gcatcttgat cgccataagt taagtttaca attttttcat tgatagtaac caactcatca
    10981 catactgaat acttcttcca aagtatatta ttaattaata gtaccccaga attaaaataa
    11041 gggctgaggt ctaaatcaag ata
//
LOCUS       DANLXQ010000083        10910 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.083, whole genome shotgun sequence.
ACCESSION   DANLXQ010000083 DANLXQ010000000
VERSION     DANLXQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.083"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(17..1306)
                     /locus_tag="PXD94_001623"
     CDS             complement(17..1306)
                     /locus_tag="PXD94_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL6286470.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHLSPKIEKEQDSA"
     gene            complement(1430..2227)
                     /gene="ccsA"
                     /locus_tag="PXD94_001624"
     CDS             complement(1430..2227)
                     /gene="ccsA"
                     /locus_tag="PXD94_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL6286471.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            2443..2967
                     /gene="yceD"
                     /locus_tag="PXD94_001625"
     CDS             2443..2967
                     /gene="yceD"
                     /locus_tag="PXD94_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL6286472.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            2984..3154
                     /gene="rpmF"
                     /locus_tag="PXD94_001626"
     CDS             2984..3154
                     /gene="rpmF"
                     /locus_tag="PXD94_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL6286473.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            3173..4195
                     /gene="plsX"
                     /locus_tag="PXD94_001627"
     CDS             3173..4195
                     /gene="plsX"
                     /locus_tag="PXD94_001627"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL6286474.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     IAPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
     gene            complement(4292..5575)
                     /gene="hisD"
                     /locus_tag="PXD94_001628"
     CDS             complement(4292..5575)
                     /gene="hisD"
                     /locus_tag="PXD94_001628"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL6286475.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPAFVASDLLSQAEHGADSQVILVTPSESLARAV
                     DEELEKQVAVLPRAETARKALAHSRTFIAENLQQAVEISNKYAPEHLIVQTENARSLL
                     ADLDNAGSIFLGAYSPESMGDYASGTNHVLPTYGYTKTYSSLGLADFSKRMTVQELTP
                     QGFLALAETVERMASAELLDAHRNAVSLRVAKLKG"
     gene            complement(5587..6486)
                     /gene="hisG"
                     /locus_tag="PXD94_001629"
     CDS             complement(5587..6486)
                     /gene="hisG"
                     /locus_tag="PXD94_001629"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL6286476.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
     misc_feature    complement(6519..6646)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            6845..7702
                     /locus_tag="PXD94_001630"
     CDS             6845..7702
                     /locus_tag="PXD94_001630"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL6286477.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
     gene            complement(7843..9324)
                     /gene="ilvC"
                     /locus_tag="PXD94_001631"
     CDS             complement(7843..9324)
                     /gene="ilvC"
                     /locus_tag="PXD94_001631"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL6286478.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
     gene            9546..10781
                     /locus_tag="PXD94_001632"
     CDS             9546..10781
                     /locus_tag="PXD94_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL6286479.1"
                     /translation="MKINRIILQSCSECGQQVRVPLTSNGNAVCPVCHNILRKSNTWS
                     LHRSAFLALAILILLPFALTFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLV
                     CAVIMPIAFVLLILTIQLQRIIRQRPRYTLIFLTKIKEWVMLDVYLVALAVAAFKIRD
                     YADLQFDINLIAFVLVTLLNTLLFIKTEPKQAWERFYPEYHALPFDHPSNPTLCPTCE
                     YCFDETILDLKGRQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTEL
                     AGQVSADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPI
                     HWQMKLLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFA
                     SANLDSRMLWKIHTVQKTS"
BASE COUNT         3238 a         2446 c         2064 g         3162 t
ORIGIN      
        1 ttcttttttc ttaaaattag gctgaatctt gctctttttc aattttcggt gaaagatgtt
       61 caactttcgc ttgtttaacc atattttcac tcacttcaag aatcatcact ttcaagttgt
      121 agagttcaaa ttccattcct tcatcaggaa ttttttctaa atgctctaaa atcaagccgt
      181 taaaggttct ggcttcctca agcggtaaat cccaatcaaa tagtttgttc agatcacgca
      241 ggttggctga gccttcaata atcaccgtac catctgattg ttgctgaact tcttgctcaa
      301 gggtcggtgc tgatgaagtc gtaaattcac ccacaatttc ttctaaaata tcttcaagag
      361 tgactagccc tttaatatca ccatactcat caaccactaa accaatacgc tctttattcg
      421 ctttaaagtt cattaattgt gtagtgagtg gtgtgccttc aggaataaaa tagacttcat
      481 ctgttgcacg cagtagcatt tctttggtaa attcgttttt ctccaacatt aaacgaaagg
      541 cttcacgcac ccgcaaaatc ccaacaatat tgttgtccat atcccctttg tagataatca
      601 cccgagcgtg aggggcgtga ttaagctgac gcataatcgc tttccagtca tcatcaatat
      661 caatgccgct gatgtcgttg cgaggcacca taatgtcatc aaccgtcact ttttccatat
      721 ctaaaataga gaccaacatt tcacggtgtt cggcaggaat aaatttaccg gcatcaagca
      781 ctacactacg cagctcttcg gagcttaagc cttgtttatc tttttgttga atccgcaata
      841 atttcatcag cccggcaata atcaaattca ttaaaaatac aatcgggctg agtaattttt
      901 taagcggtgt tagcacaaag ctggcgaaaa aaccgacttt ttcagggtaa attgccgcta
      961 ctgttttagg taggatttcc gaaaaaacca acattacaaa agttagcaaa ccggttgcaa
     1021 ttgccacacc ggcttcaccg gataactgca taccgatcat cgttgcaatc gcagaggcgg
     1081 caatgttgac taagttatta caaattaaga taaggctaag tagcacatcg gtgcgtgcca
     1141 agagtttttc cgccagtttc gcccctttat gcccttgctc ggcaaggtga cgcattttgt
     1201 agcggtttaa agacatcaag ccggtttccg agcctgaaaa aaatgcagag agtactaaaa
     1261 gaatagctaa ggtaataaat aaactactca ggggaatact gtccaaaatt caattcctat
     1321 gtaaattaat cataaaaggt agcgtaacac taaaagcgtt gtcactacct cactttgtcg
     1381 gttcagccga cttgcaaaaa attttgcttt tttgaccgct tgtagatcat taattgacga
     1441 tcaaacgact accgaaatag ccgatggtga gcaacatcat acccgaaatc gaataaataa
     1501 gcacccgttt tccacgccaa tgtaatttcc actgtccaag taactgaatg ctataaacaa
     1561 gccaagctaa aaaggagaaa attgctttat gaatatgctc cggctcgaaa aagttatgca
     1621 aataaaccac accggaaaat aaagtgagcg tcagcatagc ttgtgcagct aaggttaaag
     1681 taaagaaatg gcgttcaacc gtcattagcg gtggtagaat ctggcaaaag aacattgttt
     1741 tgctctttag ttttctatcc agccatttga gttgaaaagc ataaattaat gctaaaaaga
     1801 agagagcata agaaaataat gcaatcccta aatggaaaag cagtcccgca ttttcttgca
     1861 cattacggat aaaactacct tttacaaatc cagccacccc cacactacaa atggcgaaag
     1921 agtacacaat aattaaagga aaccaaaccg ttttccattt taacaacgca agtgtgacac
     1981 aagccgccat aatgaaactc atcaaagaga ttacatttgt taccgtaaaa ttctgccctt
     2041 cgattaaaaa taattcatta tataacacag aaaaatgcaa aatcgtggca agtaacccaa
     2101 acccaaagac taacatcata ttaggctttt tattaactgt tgcggcattt tctaaattca
     2161 ctagagtagg ggcaacccaa agtagcgcta gagcgtaagc aacaatggat aaaagaggaa
     2221 gtaacatatt gtctcgaatc tcgtcaaata agaataacta tcagaatttt aatcaaaagt
     2281 aaccgtttta gctagttttt gctgttgcta gatcaaaaat tatcctaaaa aatgtgcttt
     2341 tcgccttaaa ttgaacccta ccttttctcg gtaagattat ctcactgaaa acaataattt
     2401 tcctttgact aatttgcttt ctatctgtaa tatgcacacc ctatgcaaaa ggtaaaacta
     2461 ccccttacga ttgaccctta caaagatgct cagcgtcgcg ttgattatga aggctacatt
     2521 tcaggtaaac ttctaacacg cttgggtgaa tcagtatgta atgtgcaaag tgatgcacaa
     2581 gtcactctct cgttatatgt cgatccacaa cgcctaactg tgattaaagg cacagcaaaa
     2641 gtagatgtgg aatttgattg ccaacgatgt ggtgacccgt ttacacaaac gctcgactgt
     2701 tcatttgttt tcagtccggt gtctaacatg gatcaggcgg acatcttgcc cgaaatttat
     2761 gaacctatcg aagtaaacga gttcggtgaa gtaaatttac tagatatgat tgaagatgaa
     2821 tttattcttg aattacctct agtcccgatg catagtgaag aacactgtga agtgtccgta
     2881 agtgaacagg tatttggcga gttgccggaa gaactggtac aaaaaccaaa cccgttcgca
     2941 gtattagcta atttaaagaa aaactagatc taggagaaag cccgtggctg ttcaacaaaa
     3001 taagaaatct cgttcacgtc gtgatatgcg tcgttcacac gatgcattaa caactgcagc
     3061 agtttcagta gataaaacca gtggtgaaac tcactttcgc caccacgtaa ctgcagacgg
     3121 ttactaccgt ggtcgtaaag taatcaacaa gtaattcctt tcgaggttta tcttggttga
     3181 tatcactctt gcagtagatg tgatgggcgg ggactttggt ccccgcgtta ctattcctgc
     3241 actggaaaaa gccttaaaac aaaatccaag cctttctttt ttattatttg gtgatgaaca
     3301 ccaaatagcc cctttattac aaaaacacac actactcaac caccccaaaa tccaaattca
     3361 tcataccgaa caagtgattg aagctgatgt tccgttcact actgcaatcc gccaaagcaa
     3421 aaacagctct atgcggttag cgattgaggc agtaaaagag ggcaaagcac aaggttgtgt
     3481 tagtggcggt aacactggtg cgttaatggg attggcaaaa ttattgattg aaccattacc
     3541 aaatattgaa cgccctgcac tcacttcgct aattcctacc ctcaacggca aatccagcgt
     3601 aatgttagat ttaggggcaa atattgaagt aagcaatcac cagctacaac aatttgcaga
     3661 aatgggaaac ctgtttgctc aagtaatgct tgatttagtt taccctcgcc tgtctttatt
     3721 gaatattggt acagaagaaa ataaaggcac ggcacaactt caggcagtgc atcaactgct
     3781 gaaaaacaga caagacttaa attacaccgg ctttattgaa agcgataaat taaccagcta
     3841 tctcactgat gtgattattt gtgacggcta caccggcaat atcgcgttaa aagcgttgga
     3901 aggggcagct aaaaatatta tttctctctt taaaaaagag aaagccgatt ccaatatttg
     3961 ccgtaacacc aaacgatatt tactgaaagc gatgttctac cgttactacc gcaaattgca
     4021 acaaattaac cctgaccgcc ataacggtgc gaccttgctc ggcttatcta aagtagtcgt
     4081 gaaaagccac ggcggggcaa atatcaatgc cttttattac gctattgatt atgcggtgca
     4141 acaaattcaa ggcgacattc caaataaaat cctagagggt ttaacccaaa aataagcgaa
     4201 ctcaactgtt cgcttatttt catttcttgt ttcacttaaa gatacaagcg gtcaaatttg
     4261 caaaaaaatt tacccattca accgcttgtg tttagccttt taattttgcc actcgtaaac
     4321 taaccgcatt gcggtgtgca tctagcaatt ctgcacttgc cattcgttcc accgtttcgg
     4381 ccaaggctag aaagccttgt ggtgtcagct cctgcaccgt cattcgcttg ctaaaatcag
     4441 ctaaacctaa gctcgaatag gttttagtgt agccgtaagt cggtaaaacg tggttagtgc
     4501 cgctagcata atcccccata ctttccggcg aatacgcccc taagaaaatc gagccggcat
     4561 tatctaaatc cgccaacaac gagcgagcat tttcagtttg cacaattaaa tgctcgggtg
     4621 catatttatt actaatttcg accgcttgtt gcaggttttc cgcaataaaa gtgcgactgt
     4681 gagccaatgc tttacgggca gtttcggcac gaggtaaaac cgccacctgc ttttctaatt
     4741 cttcatcgac tgcccttgcc aagctctcac ttggtgtgac taaaatcact tgggaatcgg
     4801 ctccatgttc tgcttgagag agtaaatcgc tcgccacaaa tgcagggtca gcaaactcat
     4861 cggcaatcac caacacttcc gacgggcctg ccggcatatc aatcgccgtg ccttcttgca
     4921 caacttggcg tttcgcctcc gtcacaaacg cattgcccgg gccgaaaatt ttatccactt
     4981 tcgctaccgt ttgtgtgcca tttgccatcg caataatcgc ttgagccccc ccaactgcgt
     5041 aaatcgtttc caccccacac aaatgggcgg tgtagagaat ttcatcggca atcggcggtg
     5101 gtgagcaaag cacaatcttg ttacaaccgg caatttttgc cggcactgca agcattaaca
     5161 cggtagagaa aagcggggct gaaccgcccg gaatataaag ccccaccgca ttaatcgggc
     5221 gagtgatgac ttggcaacac acccccggct gggtttcaat atccacctct tgattttgct
     5281 gtgccttgtg gaaagcctcg atattcgctt tcgcattttg aatcgcttgc ttaagctctg
     5341 gtgaaatacg gctctgtgct tgctgaatct gctgttctga tacaactaag ctctctaatt
     5401 ttactttatc gaacttttca gctaattcaa ataatgcctt atcgccgttg gcaattacat
     5461 tcgctttaat ttcattaact gccgctttaa tactctcgcc aaccacctgt gccggacggg
     5521 caagatattg ctgctgctca gtttctgtta aattgttcca aattagggtt tgcatattta
     5581 ttccttttac gccaacattt tctcaatcgg taaaaccaat acggaacttg cgcccatttc
     5641 ttttagctgc tccattgttt cccagaacag gttttcttgg ctgacaacgt gcattgccac
     5701 acgggtttgg tcgttagcta acggtaaaat agtcgggttt tccacaccgg gtaataaagc
     5761 ggtaatttcc gccagtttgt ctttgggggc gtgcagcata atatacttag attcgttcgc
     5821 ctgttgcaca ccttgaatgc gggttaataa tttatccact aacgcttgtt tttccgctga
     5881 aagtggttct tggcgttgaa ttaggcaagc agttgaacgg taaatcactt caacctcttt
     5941 taatccgttc gcctctaatg ttgcacctga agaaaccaag tcacaaatcg cattcgcaat
     6001 tccggcactt ggagcaactt caaccgaacc ggtgagtaaa atacttttaa acggcacatt
     6061 ttgctctttc atataccgct ttaataaatt ggggtaagaa gtggcaatca cagcatcttc
     6121 taaatctttg atattgttat aatttttatc acgatcaata gctaacgata aacggcagcc
     6181 accaaaatct aactgacgaa gttttttata ggcaacattc tcaccagcac ttaatcgccc
     6241 taattccact tcctctaata cattttcacc gataatccca agatcaacta cgccgtcaaa
     6301 aactaagcct ggaatgtcgt catcacgcac ccgtaaaatt tcaatcggca tattttctga
     6361 ataggcaatc aggcgttgat cgttccaaat gattttcacg ccacattgtt taagcaactc
     6421 actacattct tggcttaagc gacctttttt ctgcatcgca attcttaatc gattttgtgt
     6481 tgtcattgtt gtttccttct tatgttttaa attgtaaaaa taaaaaaacc tctcgaaatc
     6541 acttccgaga gggttaaatt tatgctgcgt ttaggcgtac tcggaagtct tatcttccgc
     6601 acataccaaa tgcccgaaag attattcagg taaatgatgg tggtgatgag tacgaataaa
     6661 agccattttg ttgttctcca aactaaattg ctttaaaaga tacgcttaaa ttttggcaat
     6721 gacaagtgtt ttttgcgaaa aaattgctat ttttcatctc tctaaaacaa ctatcgcaaa
     6781 cgtttgcgta aagcggtata atttgcctga ttttttgcaa attcaacaaa gtaggaataa
     6841 cactatgcaa atcagcctca aaaagatcta ctccggcaaa gtccgtgatc tctacgaaat
     6901 cgatgaaaaa cgtatgctta tggttgccac cgaccgatta tctgcctttg atgttatttt
     6961 agacgatcct attcctcgta aaggcgaaat cctcacccaa atctccaact tctggttcaa
     7021 caaattagcc cacattatgc caaaccactt caccggcgac agtgtgtttg acgtgttacc
     7081 gcaagaagag gcagaagcta ttcaacatcg tgcggtggtt tgcaaacgct taataccggt
     7141 gaaaattgaa tcgattgtgc ggggttattt aaccggctcc gggctgaaag attaccaaca
     7201 aaccggcacg atctgcggct taacattgcc ggaaggcttg gttgaggcca gcaaattacc
     7261 gcaaccgatc ttcacaccgt ccagcaaagc cgaggtgggc gatcacgata ttaatatcag
     7321 ctacgaagag tgtgaacgcc agatcggtca agaacttgcc gccaaagtgc gtgatgccgc
     7381 tctaaaactc tacactgaag cggcaaacta tgccctgacc aaaggaatta ttatttgcga
     7441 caccaaattc gaattcggct tagacgaaaa cggtgaacta acgctaatgg acgaagtcct
     7501 aaccccggat tccagccgtt tttggtcagt tgaaacctac cgagaaggca caaatccacc
     7561 ttcatttgat aaacaattcg tgcgtgactg gctggaaaat agcggttgga acaaacaacc
     7621 cccagccccg aaaataccgg cagaggtaat tgaaaaaacc gtagcgaaat atcaagaagc
     7681 attggattta ttaaccaaat aacaccacaa gcggtcaaat ttacgctatt ttttgcaaaa
     7741 caaaaacctg taagccaaca cttacaggtt ttttagttag tggtaaaaag tgcggttatt
     7801 ctaccgcact tttgcaaaat tttgtgtaaa tctgaccgct tgttagcctt gcgaagaaat
     7861 acgtttcata tctgtcatat aaccacgtaa ttcttgaccg attaactcga ttgggtggtt
     7921 acggattgcg tcattcacat cacgtaaggt aacgttgtcg atttccactg ccggagttgg
     7981 ttcacctaaa tcgcctttct ctaacgttgg gataattttc tccgctaaaa tcggggttgc
     8041 aacgtgagag aataagtagt taccgtattc tgcggtatct gaaattacca cgttcatttc
     8101 atataaacgc ttacgagcga ttgtatttgc gattaacggt aactcgtgta gagactcgta
     8161 gtaagccgat tcttcataaa taccggctga aaccattgta tcaaaggcta attcaacccc
     8221 tgctttcacc attgcaacca ttaacacacc gttatcaaag tattcttgct cagaaatttt
     8281 aataccgtct gcttgtggtg cgttttcaaa ggctgtttta cctgtttctt cacgccattt
     8341 taataagttg gcatcgccgt ttgcccagtc tgccatcatc gttgatgaga actcaccact
     8401 gatgatgtcg tccatatgtt tttcaaataa gaacgctaat tcttctttga tttgctcaga
     8461 aagctcgaac gcacgcaatt tggcactgtt tgataagcga tccatcatta aggtgatacc
     8521 gccttgtttt aacgcttcgg taatggtttc ccaaccgtat tggatcaatt tacctgcgta
     8581 agccggatct ttaccttccg acactaattt gtcgtagcac acgattgaac ctgcttgtaa
     8641 cataccgcaa agaatggttt gttcacccat taaatcagat ttcacttctg ctacgaatga
     8701 agattctaat acgcccgcac ggtcaccacc ggtagcactt gcccacgctt tcgcaatggc
     8761 taaaccttcg cctttagggt cattttccgg gtgaacggca attaaggttg gcacaccgaa
     8821 accacgtttg tactcttcac gcacttccgt gcccgggcat ttcggagcgg tcatcacaac
     8881 cgttaaatcc ggacgaattt gctcaccttc ttccacaatg ttgaaaccgt gtgaataacc
     8941 aaatgccgca ccttgtttca ttaacggcat tacatcggca actactttag agtgctgttt
     9001 gtccggcgtt aagttaatga ctaaatccgc ttcaggaatt aattgctcgt aagtacccac
     9061 cacaaagccg ttttcagaag cacgtttaaa tgaagcacgt ttttcagcaa tcgcttcggc
     9121 acgtaaagcg taagcaatat ctaagcctga atcacgcata tttaaacctt ggtttaaacc
     9181 ttgagcacca caacccacaa tcacgatttt tttgcctttt aagaaattgc agccatctgc
     9241 aaattcatca cgatccataa agcggcaacg acctaactgg tctaattttt gacgtaaatt
     9301 taaagtgttg aaatagttag ccatttttta atccttattt gaattaatga gattgatatg
     9361 atgttttgtt tgcacattgt tatagtttga tttgattata ccttaaccta acattgcgta
     9421 aattgaaaat attaatattt catattgcat aaaatgcaac ttgcaaaaat tttgccaaat
     9481 ttaaccactt gcaatctcta cgataaagta gaaaataacc acctaattta atagaagatc
     9541 aaaaaatgaa aataaacaga atcattttac aaagctgtag cgaatgtggg caacaagtgc
     9601 gagtcccgct cacgtcaaat ggcaatgccg tttgcccggt ctgccataat atattacgca
     9661 aaagcaatac ttggagcttg caccgtagtg cttttttggc attagccatt ttaattttgc
     9721 ttcccttcgc acttactttt cccttgatga gcattgactt actcggcatc aaaattaatg
     9781 ctaccgtatg ggacggtatt tggaagatgg caacggaggg ctacccttac accgctttta
     9841 tggtgctagt ctgcgcggta ataatgccga ttgcatttgt attgctaatt ttaaccattc
     9901 agctacaacg tattattcgc caacgccccc gttacacgct gattttctta actaaaataa
     9961 aagaatgggt aatgcttgat gtctatttag ttgctcttgc ggttgccgct tttaaaattc
    10021 gagattatgc cgatctacaa ttcgatatca acttaatcgc ctttgtgttg gtaacactct
    10081 taaacaccct actttttatt aaaaccgagc caaaacaagc atgggaacgt ttttacccag
    10141 aatatcacgc cttgccgttt gatcacccaa gcaatccaac actttgcccg acctgtgaat
    10201 attgctttga tgaaactatt ttagacttaa aaggacggca acgctgccct cgttgtgaaa
    10261 gcaatttatc tatttccgac aacgtaaaaa tacaacgggt ttgggcttgc ttaattgcag
    10321 ggactattat gatgattccg gcaaatattt tcccgatttc gacaactgaa ctggcaggtc
    10381 aggtttcggc agattcatta atgtccggcg tgcttctgtt tatggaaatg ggtagctaca
    10441 ccgtggcagc gattgtgttt atcgctagta ttgcggtgcc ggtgagcaaa attaccatta
    10501 ttttttatct cttgctggca attcattaca agtggaaaca cccgattcat tggcaaatga
    10561 aactactaca ctacgtccat tttgtcggac gctggtcaat gctggattta ttcgtgttat
    10621 cattgatgat gtcgctactc gaacgagggc agattctcag tttctcggta ggggaagccg
    10681 ctttctattt tggagcagcc gtattcttaa cgatgtttgc ctctgcaaat ttagattcca
    10741 gaatgttgtg gaagattcat actgtccaga aaacatccta aataaagatt ttccgataaa
    10801 taatacataa catgcaaagt aagaaaagta gtacgtaacc caaaatctca atccctattg
    10861 agtaagaaag agccgataat gtagcaccga tagccagaaa gaaattagca
//
LOCUS       DANLXQ010000084        10872 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.084, whole genome shotgun sequence.
ACCESSION   DANLXQ010000084 DANLXQ010000000
VERSION     DANLXQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10872
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.084"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            25..957
                     /locus_tag="PXD94_001633"
     CDS             25..957
                     /locus_tag="PXD94_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6286480.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMALLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLAVPRIKHKLLGKRG"
     gene            959..1756
                     /locus_tag="PXD94_001634"
     CDS             959..1756
                     /locus_tag="PXD94_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6286481.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNILLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            1843..2064
                     /locus_tag="PXD94_001635"
     CDS             1843..2064
                     /locus_tag="PXD94_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286482.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            2247..4172
                     /locus_tag="PXD94_001636"
     CDS             2247..4172
                     /locus_tag="PXD94_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL6286483.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKKTG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSETLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            4246..4611
                     /locus_tag="PXD94_001637"
     CDS             4246..4611
                     /locus_tag="PXD94_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL6286484.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            4613..5299
                     /locus_tag="PXD94_001638"
     CDS             4613..5299
                     /locus_tag="PXD94_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL6286485.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMVIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            5383..7146
                     /gene="msbA"
                     /locus_tag="PXD94_001639"
     CDS             5383..7146
                     /gene="msbA"
                     /locus_tag="PXD94_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL6286486.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADGISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGLISVAKAEGNVEFKKVSFSYEGKEEKALDNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGQILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTIANNIAYAAIDKY
                     SREEIIAAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            7235..7867
                     /gene="lolB"
                     /locus_tag="PXD94_001640"
     CDS             7235..7867
                     /gene="lolB"
                     /locus_tag="PXD94_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL6286487.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNASTEVQQPTVQIPHDDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDNEGNSRSDRDVDALMQEIIGVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            7855..8709
                     /gene="ispE"
                     /locus_tag="PXD94_001641"
     CDS             7855..8709
                     /gene="ispE"
                     /locus_tag="PXD94_001641"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL6286488.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGD
                     EIEIEITESSEVELINPVEGVATEENLIYRAAKLLQKTTACRQGCRIGIKKNLPMGGG
                     VGGGSSNAATVLVGLNHLWNTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLT
                     PCDPQEKWYVVLKPEVSISTAAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKY
                     SAVEDLIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISP
                     LHQMLFQI"
     gene            8735..9685
                     /locus_tag="PXD94_001642"
     CDS             8735..9685
                     /locus_tag="PXD94_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL6286489.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGTAAQNLANPALDEIVVTDTI
                     PLSNEIKALNKVRVLTLSGMLAEAIRRISNEESISAMFDA"
     gene            9763..10773
                     /gene="pyrD"
                     /locus_tag="PXD94_001643"
     CDS             9763..10773
                     /gene="pyrD"
                     /locus_tag="PXD94_001643"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL6286490.1"
                     /translation="MYSLIRKLLFALDTEKAHQFSIQALALAGKSPFSILPTPDNPTQ
                     VMGLTFKNPIGLAAGADKNGEAIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDA
                     EGIINRNGFNNKGVDVLVENVKNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQ
                     YADYVAVNISSPNTKNLRSLQYGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDL
                     TDEEIVSVADSLIRYQIDGVIAGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLIS
                     KLSAELGERIPIIGCGGVHSLESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQL
                     NS"
BASE COUNT         3116 a         1961 c         2507 g         3288 t
ORIGIN      
        1 gatttcatta actataataa acaaatggac agtcttattc attttttgag tgccttttta
       61 actaatatca cttggattgg ggcgttagag ctggggttaa tttattccct tgttgccctt
      121 ggggtattaa tttcttataa aattctggat tttccggatc tgaccgcaga cggcagtttc
      181 cctttaggag gggcggtgtg tgtgatcagc attttgaatc aagttgatcc ttggctggca
      241 actttatacg gcacgcttgc cggagcagta gccggtatgg ttactgcaag tttgcatatc
      301 ggctttaaaa tcgaaaaatt gcttgccagt attttaatga tgatcgcact ctattcgatt
      361 aatctacgca ttatgggaat gccgaatatt gctattttag gcgaaaccac gatttacgat
      421 gtaatgccga ttgaaagcga tctacagtta gccttagtac gattagtgat ggcattgctg
      481 gttgttatta tcgtaaaagg tttgtttgac ctcttttttg cgaccgaaat cggtttggct
      541 gtgcgggcaa ccggtactaa tagtcgaatg gcgaaagcac agggtattgc gattaataaa
      601 atgacgattt tagggatggc gatttctaat gcgttaattg ccttaggtgg tgctttgtat
      661 gtgcaaagca acggtggtgt agatatttcg attggggtag gaactatcgt gattggcttg
      721 gcggcggtaa ttatcgggga agcaattttt tcggtaaaac ggattatttg gcttaccttt
      781 gcggtgatta ttggctcaat tctttatcgt ttcttcatcg cactagcatt aaataacgaa
      841 actttaaacg gcattggctt tggcccgcaa gatctgaatt taattacggc gttattggtg
      901 gtgattgtat tagcggttcc tcgaattaaa cacaaattgc tcggtaagcg aggttaatat
      961 gattgaactc aataacttat ttattacctt caacaaaggg acagcaattg agaaccctgt
     1021 gctgagaggc ttatccctaa aagttgagca aggcgaattt gtgtcggtaa ttggtagcaa
     1081 tggggcgggt aaatctacct tattaaatgc gatcagtggt gattgtgcgg tggactccgg
     1141 tgagatttta attcaaggaa ataaggtaaa ccaatccacc acttggcaac gggcaaatca
     1201 agtcgcccga gttttccaag acccgatggc tggcacttgt gaaagtttaa cgattgaaga
     1261 aaatatggca ctggcttacc aacgtggacg taaacgtggg ttgggctttg cgatcaaacg
     1321 ccaaatgcga gagttattca aagacaaact tgctttgtta gggctaggtt tggaaaatcg
     1381 gcttactgat caaatggggc gtttatcagg cgggcaacgc caagcggtga gcttgctgat
     1441 ggcatcactt cagccgtcta atatcttatt gttagatgaa cacaccgcgg cactggatcc
     1501 gaaaaccacc gattttgtgc ttgaacttac caataaaatt gtaagagaac agaaattaac
     1561 cacgctaatg gtaacgcatt caatgcggca agcccttgac tatggcgata gaaccgtgat
     1621 gttacaccaa gggcaagtgg cgtttgatgt ttcgggcgag caacggcaaa aaatggacgt
     1681 gccggatctg ttacaacttt tccaacaaag tcgccacgag cagttgagtg acgatggatt
     1741 attactcggc aattaatttt gcgatctgca tcgaaaaaat gaatttctcc ttttgttttt
     1801 ggctgtcaaa ttttaaataa taagcccaaa aggaggtatt caatgttaag cattttcaaa
     1861 acaccggtag agaaagaaac gctagacgat tgggctaaaa tttcggttga tgtagcgaaa
     1921 gttgcgattt tggcaattcc tgttatactt tttggcaaag aaccaacgtt gattaaattg
     1981 actaacttag cattattggt attgagtatt tatgtttttt tgacgcttgg gcgaaaatta
     2041 cggcagctaa aggaggtgtt gtgatggaac taggctggaa tttaaccata ggtttgctaa
     2101 ttttaaacgt ctttgcgttt gcttggcttt atattattaa ccggatttct aaagaacaat
     2161 tatccaaaaa tcaataatat ttaatttttc acaagcggtt gtttttgcaa aatattttgt
     2221 aaattcgacc gcttgcctgt ttttaaatga caaaatacga cgataaaatt cagcatgcat
     2281 ttagcgaaaa tgggttgctc agccaaaata tcagtggatt tcgaccacgc caagcacagc
     2341 ttgaaatggc tcaagtggtc gcaaaagcag taaaatttgc gacacctgtt gtggtagagg
     2401 cgggaaccgg cacaggcaaa acctttgctt atcttgtgcc ggcactactt tcaggcaaga
     2461 aaactattct ttcgacaggt tctaaaaacc tgcaagatca actttttaat cgagatcttc
     2521 ccactattca aaaagccctg aaatacaaag gcaaaattgc gttactcaaa gggcgtgcga
     2581 actatttgtg tttagagcgt ttagatcaag tgaccgcaat gggcgtactt ggggataaat
     2641 ccgttcttac cgatttaaaa aaagtcagta aatggcaaaa tgccactaaa acaggggatt
     2701 taagtgaatg tattaccatt gccgaagata gcccgattct accgcagtta gttagcacca
     2761 ccgagagttg cttaggctcg gactgcccgc attttaaaga ttgttatgtg gtacaagccc
     2821 gccgtaaagc gatggaggca gacgtagtgg tggtaaacca tcacctattc tgtgctgata
     2881 tggcagtaaa aaagaccggt tttggtgagt tgatccctga agctgagctg gtgatctttg
     2941 atgaagctca ccaactgccg gatattgcca gccaatattt tgggcaatcg ctttcttccc
     3001 gccagttatt tgatatttgt aaggatacca atattgttta ccgcactgaa ctcaaagatc
     3061 tgtctcaatt aggcaaagcg gctgatcact tgacgaaagt agtgcaagat ttccgattat
     3121 tgatgggcgg agaagggcag attcgaggca atttgcgaga gttatttcgt gataaaaaag
     3181 tggtcgatgg cttggtaaaa gtggctgaaa atattgattt tttaagtgaa gtggcgaaaa
     3241 aatcgctggg gcggtcggaa acgttggata aaattttcga gcggttagca gaagttaagg
     3301 ttcaacttaa aaaattaatg gatactagtg tgacaggcta ttgttactgg tatgaggcga
     3361 atggacgatc ctttgggctg catattacgc cgcttatggt ggcagataaa tttggtgaac
     3421 agttgaagaa ccagcaaatt ggttgggtgt ttacctcagc cacactcgaa gttgggggca
     3481 aattcgatca tttttgtcaa cggctgggca tcgaaaatgc ggagcagttg gtgttacaaa
     3541 gtccgtttga ttaccaacat caatctttac tctgtgtgcc ccgctacttg ccggatacca
     3601 ataaatccaa tactttaact gctctcgggc aattattaaa accggtgatt gaggcgaata
     3661 aagggcgttg tttcttgctt tgtacttcct attatatgat gcgaggttta gccgactttt
     3721 tacgcgaaca cagttcgctg aatgtgttat tgcaaggcga aaccagcaaa agccgtttat
     3781 tggaaaaatt tgtgaaagaa gatcacagcg tgttggtggc gacccaaagt ttttgggagg
     3841 ggattgatgt gcgaggcgat gatttatcgt tggtgattat tgataaattg ccgttcactt
     3901 caccggacga accattactc aaagcccgaa tggaagattg taagatacag ggcggtgatc
     3961 cgtttaacga tattcaaatt cccgaagcgg tgattaccct caaacaaggt gtagggcgtt
     4021 taattcgtga tgtgaccgat agaggtgtag tgattatttg tgattcccgt ttggtgatgc
     4081 gaaattatgg gcaaactttc ttgaaaagtt taccgccgtc tagacgcact agagatttgg
     4141 ggaatgttat tcaatttttg aaacaaaaat agggttacaa gcggtcagat ttctgcagat
     4201 ttttgcaaaa aaacggataa aaataaccgc ttgttgagga gttatatgtt gtctacattg
     4261 ctgcgtatgg ttgcttcttt ggcattgtta tttgtgatgc tttatttagg caagttaatc
     4321 gtttatctat tccccatcgg gatcccggat agtattttag gtatgcttat cttattcggc
     4381 tgcttggtta tcggcattgt taaagtggaa tgggtgatac cgagcgcgaa accgctcaca
     4441 cgttatataa cactattttt ccttccgatt tgtgtcgaac tcatcgaaca tcttgagctg
     4501 ttacgccaaa atctgaacac ttttgtatta gcaacggcat taagcacctt agtttctgtg
     4561 gtcgcgattg gcgtgtttgc acagtggatt tttcattaca agagagggta aaatgagcta
     4621 tctttacact gtgttgacgg taatcatgtt tattgccggt ttgaaaatca gccaaaaact
     4681 caagagcagt tttctgaacc ctttcttaat tgcactcgct ttgctaattg gtgtattact
     4741 gatttttaat atcccttacc aagactatta tcaaggtaat tcccccttga ataacttact
     4801 tggcgtttct gttgtggcac ttgcttaccc tttttatgaa caattacccc atatccgtaa
     4861 aaaatggcga cagattgtcg tgattttgct gtttagtacc atcattacga tgataaccgg
     4921 gcttgggttt tcattattat ttggggcaag ccaagagatc ttagccgcca ttttgcctaa
     4981 atcggtatca atgccaatgg caattattat tacgaatgaa attaatggaa atgttgcaat
     5041 cgcagcggtt ggggtgatga tcgcaggctt agtgggatct attttaggct ttacgcttct
     5101 gcgcctggca aaagtaagaa atgtgatggt gattgggtta agtatgggag ctgcttccca
     5161 cgcattaggc acggcaagat gcttagagca tagtatgaaa gccggtagct atagttctat
     5221 cgcattagtg gcttgtggtg tgctgtcttc tatatttgca cctttaatat ttaacgttat
     5281 cgtcacgtta tgttactaaa aacagtgaaa tctcaaacct tatttggtaa ctggctgaaa
     5341 tcaaggtaca ataccgtgat ttttcaatga aaatagagaa ttatgcaaga acaaataaca
     5401 caagaaaaag atctttctac gcttaaaacc tttaagcgtt tatggccgac tatcagcccg
     5461 tttaaagcgg gtctgattac aggtgctgtc gctttagtat taaatgcgtt agtggattct
     5521 agtcttatct atctcctcaa acccttgcta gatgaaggct ttgggcaagc taataatgat
     5581 tttctgaaat tgatggcggt gcttgtagta gtttttattt tagtgcgcgg tattaccaat
     5641 tatattgcca gttactgttt agcttgggtg tcaggcaagg tggtaatgac attacgccgc
     5701 catatttttc aacatttaat gtatatgccg gtaagctatt ttgataacaa cccgaccggg
     5761 cgtttgctct cacgagtgac ttacgatacc gaaatggtgg cgagttctgc ttctcattct
     5821 ttagtgacta ttgtgcgtga aggggcttat ctgctgtcat tgtttgtggt gatgatctat
     5881 accagttggc agctttctat tgtgttattt gtgatggctc ctatcattgg ggtactgatc
     5941 agtttcgttt caaaatattt tcgtcaatta agtcgcaata tccaaagttc aatgggggag
     6001 cttactgtca cgacagaaca aatgcttaaa gggcataagg ttgtactttc ctttggtggg
     6061 caggaaattg agaaagcacg ttttgatcga gtcagtaatg atatgcgtcg taaagggatg
     6121 aaagtcgttt ctgctgatgg catttctgat ggtgtggtac agcttatcgc ctcttttgcg
     6181 ttatctgctg tgctttatgt tgcgaccttc cccgaggtga tgagtgagaa tttaaccgca
     6241 ggttcattca cagtagtgtt ttcttcgatg atggcaatgt tacgtcctct aaaatcactc
     6301 accaatgtga actcgcaatt ccaacgtgga atggcagcct gccaaacgtt atttgaattt
     6361 ttagatttaa aaactgaaaa agatcacggc ttaatctccg ttgctaaagc tgaaggcaat
     6421 gtggaattta aaaaggtaag ctttagttat gaaggtaaag aggaaaaagc actcgataat
     6481 atttctttca ccattcctaa aggcaaaaca gtagcattgg ttgggcgttc cggctcgggt
     6541 aaatcaacta ttgccaattt gcttacccgt ttttatgatg tgactgacgg tcaaattttg
     6601 cttgatggaa ttaatattca agattaccgc ttaaaaaact taagagaaca atgctcggtg
     6661 gtttctcaac aagttcattt gtttaacgat actattgcta ataatattgc ttatgctgca
     6721 atcgacaaat acagccgaga agaaattatt gctgctgcaa aagctgcaca cgcaatggaa
     6781 tttatcgaaa aattggaaaa tggcttagac actgtaattg gtgaaaatgg cgcaagtctt
     6841 tccggtgggc aacgccaacg cttggcaatc gctcgtgcat tactgcgaaa ttctcctgtg
     6901 cttgtgctgg atgaagctac ctctgcatta gatacggagt cagaaagagc gatccaatct
     6961 gcacttgaag tgctacagaa agatcgtacg gtgttggtca tagcacaccg gttatcaacg
     7021 attgaaaaag ccgatgaaat tttggtagtc gatcacggaa atattattga gcgtggcagc
     7081 catacggaat tgttagcaca aggtggggct tataaacaac tccatagttt acaattcgga
     7141 gcctaatttg taggggagaa atatgtttct ccccaattaa tgataaaaac agggcgaaaa
     7201 atatttcgtc cctacaataa tttatttgga tactatgaaa aaactgacaa aacttttatc
     7261 tcttagtgct ttactggtct taaccgcctg taattcggta ctaaatgcct caacagaggt
     7321 gcaacaacca acagtacaaa ttccgcatga cgatccacaa tggcagcagc atttagcgaa
     7381 actgagccaa attcaaggct atactgcaac ggggcaattt ggctatattt cgcctgaaga
     7441 gcgtttttct tcccatttca actggcgtta tactaatcca atccaatttg ggttagagct
     7501 ttcctcgaat ctctcgacta aatcactgaa attagagcgt aataaacgag gcttaacggt
     7561 ttcggacaat gagggtaata gccgttctga ccgtgatgtt gatgcgttaa tgcaagagat
     7621 tatcggtgtt tcgtttccta tcgatcagtt tgcctattgg gtaaaaggct tgcctgaaaa
     7681 agatggcaac tatattgtga atgaaaaacg ccagctgtcg caatttagtt atccgcttaa
     7741 tggcacgctt tggaaagcaa gctatgtgga gtatcacgaa gatcgtgtac caaacttacc
     7801 gaagctgatt gtgcttgaaa acggcactca aacgttgaag atccgcattg aaaaatgggc
     7861 gtattaatga agaaagtcac cttacccagc ccggcaaaat taaacctgtt tttatacatt
     7921 accaataagc gggcagacgg ttatcacgag ctacaaacgc tgtttcagtt tttagatttt
     7981 ggtgatgaaa ttgagattga gatcacagaa agttcagagg ttgaattaat taatccggta
     8041 gaaggggtgg caactgagga gaatctcatt taccgagcag caaaactttt gcaaaaaacg
     8101 accgcttgta ggcaaggctg ccgtattggg attaaaaaaa acctaccgat gggcggaggt
     8161 gttggtggtg gatcttctaa tgctgcaaca gtattagtcg gtttaaatca cttgtggaat
     8221 accgagcttt cattggaaaa attggcagaa ttaggcttaa gtttaggggc tgatgtgccg
     8281 atatttgtta gaggtatggc tgcttttgcc gaaggcgtgg gggaggtgtt aactccttgt
     8341 gatccacaag aaaaatggta tgtagtgcta aaaccagaag tgtcgatttc aaccgcagcg
     8401 gtgtttaccg acccgaattt acctagaaat acgccaaaac gccccttagc cgaattatta
     8461 tctttaccgt gggaaaacga ttgcgaaaaa gttgtacgag aaaagtattc tgcggttgaa
     8521 gatcttatac aagaattgtt acaatatgct cagtttaggc taacaggcac aggtgcttgt
     8581 attttcgccg aatttgaaac aaaagcagaa gccgagcagg tttttgcaca taaaccggaa
     8641 gccgtcttcg gttttgtggc gaaagggcaa aatatttcgc cattacatca aatgttattc
     8701 caaatctaac catcttaacc ttaaggatca atccatgaca gatattaaac tgtttgcggg
     8761 caacgcaact ccggaacttg ctaatcgtat tgcaaaacat ctttatacct cattaggtaa
     8821 tgcaacagtt ggtcgtttta gcgatggtga aattcaagta caaatcaatg aaaatgtgcg
     8881 tggaggcgat atttttattg tgcaatccac ttgtgcacca actaatgata atttaatgga
     8941 attgatcgtg atggtagatg cgcttcgccg tgcatcagct ggtcgtatta ccgcagtgat
     9001 tccgtatttc ggttatgcac gccaagacag acgcgtgcgt tcggcccgtg tgccaattac
     9061 cgcaaaagtg attgctgatt tcttatccag cgtaggggta gatagagtct taacctgcga
     9121 cttacacgca gagcaaattc aaggtttctt tgatgtgccg gtagataacg tatttggttc
     9181 ccctgtgtta atcgatgata ttttgaaaaa atccgatttg gtcaacccta tcgtggtttc
     9241 tccagatatt ggtggggttg ttcgtgcaag agcggtggca aaattattaa atgataccga
     9301 tatggcaatt attgataaac gtcgtccaaa agcgaatgta tcacaggtaa tgcacattat
     9361 cggtgatgtg gctgatcgtg actgtatttt agttgatgat atgatcgata ctggtggtac
     9421 tttagtcaaa gcggctgaag cgctgaaaga acgtggcgcc cgtcgtgtct ttgcttatgc
     9481 cacacacgcg gtattttcag gcacagcagc tcaaaattta gcgaatcctg ctttagatga
     9541 aattgtagta actgatacta ttccactgag taatgagatt aaagcattaa ataaagtacg
     9601 tgttttaaca ttatccggta tgttagcaga agcaattcgc cgtattagca atgaagaatc
     9661 catttcagca atgtttgatg cctaatttat gagaaaaaac ccagtcgaaa gattgggttt
     9721 ttcatatctg ttcactacta tatttagttt aagataattt gtatgtattc cctgattcgt
     9781 aaattacttt ttgcactaga tacggaaaaa gcacatcaat tctctattca ggcacttgct
     9841 ttagccggaa aatcgccttt ttcgattctg ccaacacccg ataatccgac ccaagtaatg
     9901 ggactcacgt ttaaaaatcc aatagggctt gcagccggag cggataaaaa cggtgaagcg
     9961 atagatggtt ttgggaaatt aggatttggg tttattgagg ttgggactat tactcctctc
    10021 gctcaagacg gaaaccctaa accgaggcaa tttcgtattg tagatgcaga gggaattatt
    10081 aaccgcaatg gatttaataa taaaggcgtt gatgttttag tagaaaatgt aaaaaatgca
    10141 acctatagtg gtattttagg tattaatatt ggaaaaaatg cggttacacc aattgagcat
    10201 agctttgatg actaccaaat ttgtttgcgt aaggtttacc aatatgctga ttatgtggcg
    10261 gtgaatattt catcacccaa taccaaaaat ttgcgcagtt tacaatatgg tgaggcatta
    10321 gatgatttgc tgcgaaacct caaaaatgag caggcacaac tttcgcaaaa aatggggaaa
    10381 tatcgaccgc ttgttttaaa gattgcgcct gatttaaccg atgaagaaat cgtttctgta
    10441 gccgatagtc ttattcgtta ccaaattgat ggggtgattg ccggcaatac taccttgtct
    10501 cgtgaaagta ttgccggatt accgtattcg gaacaactgg gtggattaag tggcaagcca
    10561 ttacactcat taagtacaag gttaatttca aaattatctg ccgagttggg cgagagaata
    10621 ccaattatag gttgtggggg agtccattcg ttagagagtg ggcaggagaa agtgaatgca
    10681 ggtgctagtt tattgcaagt ctattccgca atgatttacc aaggaccatc attaattcaa
    10741 accttagcca aacaccttca gctgaacagt tagggataga tttctagtta aaaatgattg
    10801 gttaggttta tttctgtcat cttagtggaa tagatggcgg tattagctat ctatcaaaaa
    10861 tagacaaaat tg
//
LOCUS       DANLXQ010000085        10468 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.085, whole genome shotgun sequence.
ACCESSION   DANLXQ010000085 DANLXQ010000000
VERSION     DANLXQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.085"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            17..1525
                     /locus_tag="PXD94_001644"
     CDS             17..1525
                     /locus_tag="PXD94_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL6286491.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
     gene            complement(1590..1811)
                     /locus_tag="PXD94_001645"
     CDS             complement(1590..1811)
                     /locus_tag="PXD94_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL6286492.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(1840..2349)
                     /locus_tag="PXD94_001646"
     CDS             complement(1840..2349)
                     /locus_tag="PXD94_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL6286493.1"
                     /translation="MLFTGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVALLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIIYAGGCATGTWYRAGEG
                     LIGSWVALVIYGLFSASMRTGVLAPLNQELKSNVIQHRTIYETFGISPWVLVFILSVI
                     TLALSAHSI"
     gene            complement(2456..3790)
                     /locus_tag="PXD94_001647"
     CDS             complement(2456..3790)
                     /locus_tag="PXD94_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL6286494.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFNVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALFSTLVSTFLIGALIYGVARLLGLELGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQEPNLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLKIMTWGGLRGGLALAMALSVPAGAAVVSSSEGNLDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRKVALNKLGAPDDVIPHI"
     gene            complement(3901..5049)
                     /gene="dapE"
                     /locus_tag="PXD94_001648"
     CDS             complement(3901..5049)
                     /gene="dapE"
                     /locus_tag="PXD94_001648"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL6286495.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKLGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTE
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYVQFNLRYCTEVTDEIIKAKV
                     AEMLEKHGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVTKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNATIHKVNECVSADDLAHCGEIYYQILEKLLATA"
     gene            complement(5179..5526)
                     /locus_tag="PXD94_001649"
     CDS             complement(5179..5526)
                     /locus_tag="PXD94_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL6286496.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            complement(5526..6384)
                     /gene="folP"
                     /locus_tag="PXD94_001650"
                     /pseudo
     CDS             complement(5526..6384)
                     /gene="folP"
                     /locus_tag="PXD94_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
     gene            complement(6493..7455)
                     /gene="rluC"
                     /locus_tag="PXD94_001651"
     CDS             complement(6493..7455)
                     /gene="rluC"
                     /locus_tag="PXD94_001651"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL6286497.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRNKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     ESMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDAEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            7564..8466
                     /locus_tag="PXD94_001652"
     CDS             7564..8466
                     /locus_tag="PXD94_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL6286498.1"
                     /translation="MTEKVTETAPNIDVVAETEKVIHKVSNMNIETMLNEWIIPYGTK
                     ILLAIAIFVVGKFLARLISRLLGKAALKSTKDEMLQSFIVSISYFLLLLIVVIASLSQ
                     IGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGVVE
                     QMGLLVLELRTGDNKTVLLPNGKVFSDSIINYSNNPTRRIDFTFDISYESNLKEAKEI
                     IQRILDANTYVLKDPAPVVAVGALAPHSVQLVVRPWVQTPNYWAAYWEIIEQVKLAFG
                     EAGISIPYNQMELHIKSGSLENKA"
     gene            8564..9061
                     /gene="rraA"
                     /locus_tag="PXD94_001653"
     CDS             8564..9061
                     /gene="rraA"
                     /locus_tag="PXD94_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL6286499.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIAEVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYVYADLTGIILSQELLDL
                     NDISE"
     gene            complement(9109..10452)
                     /locus_tag="PXD94_001654"
     CDS             complement(9109..10452)
                     /locus_tag="PXD94_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL6286500.1"
                     /translation="MKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQKNVIAE
                     SAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAFGVSGGS
                     ILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGDLLQERL
                     NFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDLEISRAV
                     AASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHILSLYQDS
                     KERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYQYIHQLRVKNIVVINVNAQNEVS
                     SEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSKIEMHFV
                     SLGLKELPDGDLKKDVLNIATSFYLPEEDVDKLREAARVLLQQSQEYNAALKTLQ"
BASE COUNT         3060 a         2349 c         2045 g         3014 t
ORIGIN      
        1 ttatgaggat tctattatgt ctaaatgtcc tgttacacat ttgacaatga acaacggtgc
       61 acctgttgtt gataaccaaa acagcttaac agcgggtcca cgtggtccat tattagcgca
      121 agatttatgg ttaaatgaaa aattagcgaa cttcgtgcgt gaagtcattc ctgagcgtcg
      181 tatgcacgct aaaggttccg gtgcgttcgg tactttcact gtaactaacg acatcactaa
      241 atacactcgt gcggcaattt ttagccaagt cggtaaaaaa actgaaatgt ttgctcgttt
      301 caccaccgtt gcaggtgaac gtggtgcggc agatgctgag cgtgatattc gtggtttcgc
      361 attaaaattc tataccgaag aaggtaactg ggatatggtg ggtaacaaca cccctgtgtt
      421 cttcttacgt gacccacgta aattcccgga tttaaacaaa gcggttaaac gtgacccacg
      481 cacgaatatg cgttctgcaa ctaacaactg ggatttctgg acattattac cagaagcatt
      541 tcaccaagtg actattgtaa tgagtgaacg tggtattccg gcttcatatc gtcatatgca
      601 cggttatggc tcacatactt atagcttcat caatgcagct aatgagcgtt tctgggtgaa
      661 attccatttc catacacaac aaggcattaa aaacttaact aacgaagaag cagccgctat
      721 tatcgctaac gatcgtgaaa gtcaccaacg tgatttatac gaagcgattg agcgtggcga
      781 tttcccgaaa tggaaaatgt ttgttcaaat tatgcctgaa actgatgcag acaaagtacc
      841 ataccacccg ttcgatttaa ctaaggtatg gccgaaatca gactatccgt taattgaagt
      901 gggtgagttt gaattaaatc gtaaccctga aaacttcttt gctgatgtgg aacaatctgc
      961 attcgcacca agtaacttag tgccgggcat cagcgtgtct ccagaccgta tgttacaagc
     1021 tcgtttattc aactatgcgg atgctcaacg ctaccgttta ggtgtgaacc accaccaaat
     1081 ccctgtaaac gctccacgtt gcccggttca tagcaacgct cgtgacggtc aaggtcgtgt
     1141 ggacggtaac tacggtagta ccattcacta cgaaccaaac agcttcggca aatggcaaga
     1201 acaagctcaa tatgcagagc caccactgaa aattaatggt gatgcagccc actggaacta
     1261 tcgtgaagac gatgctgact acttcagcca accacgtgta ttgttcaata tgatgaatga
     1321 tgaacaaaaa caagcattat tcaataatac tgctgcaggt atgggcgatg cattagactt
     1381 cattaaatac cgtcatattc gtaactgcta tgcctgtgat ccggcatatg gtgaaggtgt
     1441 ggcaaaagca ttaggtatga cggttgaaga tgctattgct gcaagagcaa cagacccagc
     1501 attaggtcaa ccgggtttac tctaatcctt agtaaataca aaactcaaag caaaagctct
     1561 acaaaaaagt agagcttttt tattgctaat tacttaacca ccgtaatttc ccacaaggca
     1621 tcaccaagtt gttggtaatc ggtaacttca tagccttcct cttccgccca atttggaatg
     1681 gcttcggttg cttgagtaca gtcaaattca atcgttaagc tatcgccttt attcaatttt
     1741 gccatcgccg ctttggcttc gactaatgga aacgggcaaa caagccctga ggcaggtaaa
     1801 cgtacgttca tctttattac tccttaaaaa attaaccttt taaatgctgt gggcggataa
     1861 tgccaatgta attacactca ggataaacac taacacccaa ggggaaatac caaatgtttc
     1921 ataaatcgtg cggtgttgaa ttacattgct ttttagctct tgattaagtg gggctaacac
     1981 accggttcgc atagaagcag aaaacagccc ataaataacc agtgcaaccc aactgccgat
     2041 taaaccctca ccggcacgat accacgttcc tgtagcacaa ccgcctgcat aaataatgcc
     2101 gataccaaac agaaatgccc caatgatcac tgcgagcaaa ggcaaatctt ccgccggaga
     2161 actaaaagag ccggcttcat acaaaagcca aatcccgatc gattgcaccg taattgcaat
     2221 taaaagtgcc acaaacatac ggctattctt tgttacatag agatcacgaa atgctccggt
     2281 aatacaaaaa cgccctcttt gcatcacaaa cccgagtaga aagccacata ataatcctgt
     2341 gaataacata aaaaatacct ctcaacaaat taacgtgaga ggtatttaaa tataaagccg
     2401 tctagatggc taataatctt tagcaaaatg atatgttatt cagttataaa tgctattaaa
     2461 tatgtggaat tacatcatca ggtgcaccta atttattgag tgccactttt cttggatcta
     2521 ccaacttgga tttttttatc atcggttcaa ccgttgaacc ttgtactaaa atcgagaaag
     2581 tgactaccgc ataagtcatt aacaaaatca aatcacgtac atcaagattg ccttctgagc
     2641 tcgataccac cgcagcccct gccggtacgg aaagtgccat cgcaagggct aatccaccac
     2701 gtaaaccgcc ccaagtcata atttttaagg tgtacgggtt ataagtacgg aaacgtttta
     2761 acacttggta tggaatccac aggctgatat agcgtgcaag caaacaaatt ggaattgctg
     2821 ccaacattaa caccccacca ataaaggtga aatcaactaa cagtgtactt aagccgataa
     2881 gcaggaataa caggtagttt aatgaatggt cgatcatctc ccagaagtga tcaagatatt
     2941 tttggctctg ttcggagaaa ccggaacggc gagtccagtt gccaatcata ataccggcaa
     3001 ctaccattgt taatgcccct gaaacgtgta ggtagtttgc aatcacaaag ccgactgttg
     3061 gaatcgttag cgtaattaaa atttcaaggc ttccgtcatc ggttgcggaa ataaatttat
     3121 gtgccacaaa accggttaat aagccaaaaa cgataccgcc taccgcttct tgcaagaaca
     3181 gtccggtaat accgctaagg tttggttctt gtccgccaaa agcaacggca aagatagtgg
     3241 taaaaatcac caagccgata ccgtcattaa acagagattc gccctccacg tgcatcgaaa
     3301 ggcgttttgg ggctttcagg cttttgataa ttgccaatac ggcaatcggg tcggttggcg
     3361 aaatcagtga gccaaacagc aaacaataca caaagccgag ctctaaccca agtagccttg
     3421 ccacgccata aattaacgca ccgatcaaga aagtagaaac taaggttgaa aataaggcat
     3481 aaactgcaat ttcccgccgt tgctctttca ttaatggtag tttaatccca agtgaaccgg
     3541 caaaaagcaa gaaccccagc attccgttta acaggaagct tttaaaatcg agttgttcaa
     3601 aaatggaaat ggcaagttta tcaacattaa accagccgag agaaccgaag gtaagaataa
     3661 gaagggagcc gacaagggca gaggctgtaa ttgcaatagt ggtttgaata ttttcgttta
     3721 attttcgggt aaaaaacgaa ataaacatcg caagcccaaa cagcaagcag atataagagt
     3781 agagttgcat aacatttacc tataaatata aaaactgccg ctagtgtaaa cgctgtttaa
     3841 cagaatagct acataaaaaa taggcagaat acaatttgat aacattctgc ctatttaggg
     3901 ttatgcagtt gccaataatt tttctaaaat ttgataataa atttcgccac aatgtgccaa
     3961 atcatccgca ctcacacact cattcacttt gtgaatagtg gcgttcagtg gtccaaattc
     4021 cactacctct gcccccatta atgcaataaa acgaccgtcc gaagtaccgc cacctgtgtc
     4081 taaacgaggc gtaatttttg tgacattttc gaccgcttgt aaggcggctt cgaccaattt
     4141 tccattgccg gcaaggaatg gtttgcctga taaattccaa cttattcggt atttcaaacc
     4201 gtgtttttcg agcatttccg ctactttggc tttgatgatt tcatcggtca cttcagtgca
     4261 ataacgtaag ttaaactgca cataaagctc gcccggaatc acattattac tgcctgtgcc
     4321 ggctttgata ttggcaattt gcaaactggt aggcgggaaa aattcgttac cgttatccca
     4381 ctgataagtg gtcagctcgg ttaaaaaacc cagtgacgta tgcaccggat tttccgccaa
     4441 atgtggatag gcaacgtgcc cttgaatgcc ctcgatatag agatctgccg taattgaacc
     4501 acgtctgccg tttttaatca catcgccaaa caccttgcta ctagacggtt cgcccactac
     4561 cgcataatgt accgcttcat tgcgagccat taaggtttca accactttta ccgtaccgtc
     4621 tttggctgct gcctcttcat cggaggtaat caataatgcc actttgccct tatggtttgg
     4681 gtgatgttgc acgaaagttt ctgccgccac aactaacgcc gagagcgagc ctttcatatc
     4741 tgccgcccct cgcccataaa gcattccatc tacaatctca gccgaaaacg gcggataagc
     4801 ccattggctt tcatcgccta ccggcaccac atctgtatgc ccggcaaaca caatgcaagg
     4861 ctctgtttta ccattttccg agccgtgcgt tgcccataaa ttcaaggtgt cgccaaacgg
     4921 caaccattca atattaaagc ccagtttttc aagacgttgt gcaatttctt gctgacagcc
     4981 tttgtcttgt gggctgatgg aatcacggcg gattaggttc tgtgcaaggg aaataatttc
     5041 atttttcata ggttacaagc ggttgttttg ggtgaatttt ttgctatata tcctgtaggg
     5101 gcgggtcctg tgcccgcccg aacaatccta aagactacgg acaggcacag gacctgtccc
     5161 tacaagcagg ggaaaaaatt aattaaacgc agccacatac tctttctcat taaaaccaat
     5221 caaagcaata tcctctttaa tcacaatcgg gcgtttaatt agggtcggct gttcggttag
     5281 cacttttaat gccgactcac ggttaaggtt gtttttaatt tcctcatcaa ggtttcgcca
     5341 agtggtgcta cgtttattaa ctaagttttc ccaaccgaat tttgcttcca tttccactaa
     5401 ccaatcggca tttaaaccgt ccacacggta atcgtgcagt tgtgggtttt gattatgatc
     5461 ttcgagccat ttcaaggctt tttttactgt gtcgcaattt ttaatgccat aaactaaggt
     5521 tgtcatcatt tttcctcatt atctgattcc agcattgcct gccagatttt taacgcatcg
     5581 gcggtttctg ccacatcgtg cacccgtaaa attttcgccc cattttgagc ggctaataac
     5641 gcccctacta cagagccaag tagccgttgt tctaccggtt tatctaacac cgcaccaatc
     5701 attgatttac gggaaagtcc ggcaagcaca gggaagcctg aatccacaaa ggctttaagg
     5761 tgtttcaata attggtaatt gtgtttcacg cttttaccaa agccaaaacc ggggtctaaa
     5821 ataatgtgtt ctttgggaat gcctgcggtg atgcactcga aaatccgttg atttaagaaa
     5881 tctgccacat cttccaacac atcatcatag tgcggatttt gttgcattgt ttgcgggttg
     5941 ccttgcatat gcattaaaca aaccggcaag cccaattcta ctgccgctgc caacgcattc
     6001 ggctcggtta aggctcgaat atcgttaatg atatccatac cgacttttgc cgactctttg
     6061 aatacttctg ctttggagct atcaaccgaa atcaagcaat caaaacggct gcggatcgcc
     6121 tccaccaccg gcacaacacg ctctaattct tgttctaagc tcacaatcgc cgcattaggg
     6181 cgggtggatt caccgccaat atcaataatg cttgccccca tcagctaaca ttttttccgc
     6241 ttggaacaag gctttatcga gggaaaaaaa cttgcccgaa tcggaaaatg aatccggcgt
     6301 gaagtttaag atccccataa tttgcggtgt ggttaaatct aacaagcggt catttttttg
     6361 aaaatttttg aaatggattt gcatattttt gcttatattt tgttggtgca agcgtccacg
     6421 cttgtaccac tctatgttga tttacagcac aagcgtggac gcttgcgcta tcttactctc
     6481 tcattaagtt atttagtaat tagcccttaa ttttgccaat acgcctttta aagttttatc
     6541 cagcggggcg gtaatgacca tttcttcgcc ggttttgggg tgttcaaaac gaatagcatg
     6601 ggcgtgcaga aataaacgct gtaagcccac ttctttcatt ttgctgtcaa attccgcatc
     6661 accatatttg tcatctaacg caatcgggtg tccggcatat tgggtatgca cacggatttg
     6721 gtgggttctt ccggtaatag gcgaagcttt caccaaagtg gcattggcat aacgctcttc
     6781 aatgctgaaa cgggtttcgg acggtttacc ctcttcactc acccgtacaa ttcgctcacc
     6841 gctggcaagt tcgtttttta gcaatggagc tttaatcact ttggtatgag cctgccattg
     6901 ccctcgcaca agggcaagat agtctttttg tactactttt tcacgcagtt gttcgtgcaa
     6961 ggaacgtaac gccgaacgct ttttcgccac cagcaaaatg cctgatgtat cacggtctat
     7021 acggtgtacc aattccaaga aacgagcctg tggacgtaat gcacgcaagg cttcgatcac
     7081 accaaaactt aaaccactgc caccgtgtac tgcaatgcct gaaggtttgt taattactag
     7141 cattgactca tcttcataca gaatttggct ctccagctcc gccactttgt ttaatttagt
     7201 cgaaattggg gcttcatttt tttccgccac acgcaccggc ggcacacgca ctaaatcatc
     7261 aggctgtaat ttatattcgg gtttgtttcg tcctttattc acccgcacct cgccttttcg
     7321 cacaatgcgg taaatcaaac ttttcggcac gcctttgagc tttgccaata aaaaattatc
     7381 taaacgctgt ccggcttcat cttcactgat cgtaaaaaat tgaacgccgg cactaattac
     7441 tttttctgtt gtcattatct tgtcttttag agaaaatttt gctctaaatt ctagcacaaa
     7501 accgcttagc gagtgaggca aattcgctag gcttattttt cattttaatt ttaaggaaac
     7561 aatatgactg aaaaagtcac cgaaacagct ccaaacattg acgtggttgc cgaaaccgaa
     7621 aaagtaatcc acaaagtcag taatatgaat attgaaacta tgctaaatga gtggattatc
     7681 ccttatggca ccaaaatttt actggcgatt gcgatttttg tagtcgggaa atttcttgcc
     7741 cgcctaatta gccgtttact cggtaaagcc gcattaaaat caaccaaaga tgaaatgctg
     7801 caaagtttta ttgtctcaat cagctatttt ttactgctgt taattgtggt gattgcctcg
     7861 ctttcacaaa tcggcattaa caccagctcg ctggtcgcat taatcggtgc tgccggttta
     7921 gccatcggtt tatcattaca aaactcatta caaaactttg ctgccggcgt gatgttgtta
     7981 atcttcaagc catttcgcaa aggggattta atcgaaaccg gcggaatgac cggcgtggtt
     8041 gaacaaatgg ggcttttagt gttggaactt cgcaccggcg ataacaaaac cgtgttatta
     8101 ccaaacggca aagtgttttc tgacagcatc atcaactact ctaacaaccc gacacgccgt
     8161 attgatttca cctttgatat ttcttatgaa tctaacttaa aagaggcgaa agagattatc
     8221 caacgcattt tagacgccaa tacttatgta ttaaaagatc cggcacctgt agtagcagtc
     8281 ggcgctttag caccgcatag tgttcaactt gttgttcgcc cttgggttca gacaccaaat
     8341 tactgggcag catattggga aattatcgaa caagtaaaat tagcctttgg tgaagcaggt
     8401 atatctattc cttacaacca aatggaattg catattaaaa gcggttcact agaaaataaa
     8461 gcataagaaa aaagcctgta agcggtcttt tttcgataaa attttgcaaa ttttgggcga
     8521 aggtttatac tttagctaaa taatctacga acaggagcat attatgcgta ttgatacctc
     8581 tgcactttgc gatctttatt ctgaccaagt tgatgtggtt gaaccaattt tctcaagctt
     8641 tggcggtgct tcttccttct ttgggaagat taccacggtt aaatgctttg aaagcaacgg
     8701 tttaattgct gaagttttgg aagaagaggg tgaagggcga gtgctgttag tcgatggtgg
     8761 cggtgcggtt cgtcgtgcct taattgacgc agagctggct caacttgctg tagataacgg
     8821 ctgggaaggt atcatcgttt atggtgcggt tcgccaatta gatattttgg aaagattaga
     8881 tatcggtatt catgctttag cgccaattcc agtgggagca gataataccg acattggcga
     8941 agtggatacg cctgtgaatt ttggcggtgt aaccttcttc cccgaagatt acgtttatgc
     9001 tgacttaacc ggcattattc tttcgcaaga gttgttggat ttaaacgaca tcagcgaata
     9061 aatatttgca aaaaaatacc aaaaaccgac cgcttggtcg gttttttact actgcagtgt
     9121 ttttaatgcc gcattatatt cttgagattg ctgcaataaa acccgagcag cttcacgtaa
     9181 tttatctaca tcctcttccg gcaaataaaa tgatgttgca atatttagca catctttttt
     9241 taaatcacca tcaggtaatt cttttaaacc taaactgaca aaatgcattt cgatctttga
     9301 tggctgttta tttgcatatt cattccaacc atctgtaaac tggcgataac gttgtaaatt
     9361 aatttgtgtc gttctatcaa ttggcacatt aactattgta ttcactacat cagccacccc
     9421 cggtacattt gcggtcttat cgatctcgct agatacttca ttttgcgcat ttacattaat
     9481 cactacaata tttttcaccc taagctgatg aatatactgg tacatctctt caatacccat
     9541 aacatcagat atttccagca cactggcaag ccctaaatta tctgttaatc caccatcaac
     9601 taaatggata aaggggcgtt ctttactatc ttgataaagg ctgagtatat gattcgtttc
     9661 ttcaatattt tttgtaatta gtccatcaat tgctttttct ctagcactaa attcttgtgg
     9721 catactaaaa tggcaatttc caccattgtt attcagtgta agcggcgcaa aaatcagagg
     9781 aacagaacta gaagcagcaa cagcacggga aatttctaaa tcattcaaat ttaaacaaag
     9841 cccatcaaag gtttcttgcg taaaggtcag tttttgacca atgttcatgt ctgttgcgct
     9901 aatcacagca aaaggtcctt tacgattttt cactaaatca ccaaactttt taccatgata
     9961 aagggcaaaa tttaagcgtt cttgcaataa atcaccacgc ccatattgag gagaacttaa
    10021 acgaggaaga ttggataacg aaaacacctc tgtaataata tctttttgaa agttcgcttt
    10081 taaaaaacgg tcagcaaatt taggaatgat atcctttcct tccaacgcaa aataagtggc
    10141 taatatcgat ccacctgata cgccaaaggc aatatctata ttctctaata aagtattacc
    10201 tttatttgtt tctttcaccc ttaccttttt aaactcttcc aacactccat aaccaagcgc
    10261 cgccgctctt gttccaccac ctgaaaacat gatgacaaca atattaccgt cttttgcact
    10321 ttcagcgatc acattttttt gtcgataacc tctttcaaca tttacctgtt taattgtttc
    10381 aactggctta taaacaacca aactgcaagc agataacact agcgtaaagg cgaagatgaa
    10441 aagctgtttt attttgttta gaaaaagc
//
LOCUS       DANLXQ010000086        10426 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.086, whole genome shotgun sequence.
ACCESSION   DANLXQ010000086 DANLXQ010000000
VERSION     DANLXQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.086"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(16..813)
                     /locus_tag="PXD94_001655"
     CDS             complement(16..813)
                     /locus_tag="PXD94_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL6286501.1"
                     /translation="MENFEKQFISLFQRIVPHYRRSEVFYDFITLSALDMYLVTYRDQ
                     AEPNLRERFAHAKARYSDSEFTQLAELLAVTVNALTQKRYDFLGSVFMNLNLGDGYRG
                     QYFTPSHIADLMAKVTLQDCDRIISQQGFVTLSEPTCGSGVMVIGCVNAMFDAKYNPQ
                     QQLWVHCQDVDFTAAMMCYIQLSLLHIPACIVVGDTLLNETKIQMYTLAHLMGNWSHK
                     LEQQIAPLVNSTTEKGCGNNIEKSVIVEDLSQISILDEIDDNELIFY"
     gene            complement(886..1632)
                     /locus_tag="PXD94_001656"
     CDS             complement(886..1632)
                     /locus_tag="PXD94_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286502.1"
                     /translation="MPNWCENKLQIFCDCIQAKQIIPKLFRKTESDKWCLDFELLVPM
                     PESICIESSSLGDRAMEFLKISPNRRLTRPLLKKYIYGSEIDRIYAKAKHHRWDVGHF
                     INWLIKRPEQQSILWLNLALGQQYLSNLAQHGYKDWYDWSNEQWGCKWNVCSDSCQVS
                     FNDNGSINCLFDTPWGPPDGWFQALCTAFPDIEFILSFFEPGMWFAGKYIANLDGSYC
                     NIYIDDRGIRSFAEEVFNEEFEDEFEEKLE"
     gene            complement(1772..2746)
                     /locus_tag="PXD94_001657"
     CDS             complement(1772..2746)
                     /locus_tag="PXD94_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL6286503.1"
                     /translation="MNTQTKFDLYQHITDRIVAALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEYGYKQSKWITAKKANELGGYIRKGEKATVVMNYRPDK
                     REKLDEEGNIVYDEEGNPEIEQHFFIKKHNLFNLDQCENLPEELYDPISEPKQPTSDP
                     NQYEIFADIRQIIKGMDLHVEMKPSNRAYYQPSADLVVMPEIKQFENAQAFHSVLLHE
                     MTHATGAAKRLDREGITSGKAKFGNKVYAFEELVAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVIKEDKRAIVRASGKAREACQYMLDALNISQLADKNEVFEEAA"
     gene            complement(2991..3398)
                     /locus_tag="PXD94_001658"
     CDS             complement(2991..3398)
                     /locus_tag="PXD94_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6286504.1"
                     /translation="MKHDLDLLKYIWDNRNLNNKAYQYLFHICIEPMRKGDWNRYTIW
                     LNNNGNLVNISAVVYEIVSSQQLKDDIKLWQDMQFHCRPVKVETTGFISFLLHQFFNG
                     ITHVLLEQGMITETEAAEKSYHFIAANSFQRLL"
     gene            complement(3514..4356)
                     /locus_tag="PXD94_001659"
     CDS             complement(3514..4356)
                     /locus_tag="PXD94_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286505.1"
                     /translation="MSGSVLNTYFKHALVKAGFPNCLNIEYSLSHCQGDGVAFYGRLN
                     TETLINLFNKLNPQKRTQKMFSNLLNHIESWEPHYGVDFEIIRNSFGYRYSHCNTMEL
                     SVRTADDLAFFYDRDARKEWYFPTSKVEKYKALWDGFIQDLGQYIKDTSCALESNGYQ
                     IIEASPYQTETIFKFNTENYLIELKRKSSEFYSESVDWLCGDVCDFEAMIEEILQGKL
                     CYADFVATVTDKESGIQLGSDEITGVSYSPTDHSLSGYRQELISNAIASARSNAARFA
                     YLHA"
     gene            complement(4434..4829)
                     /locus_tag="PXD94_001660"
     CDS             complement(4434..4829)
                     /locus_tag="PXD94_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286506.1"
                     /translation="MARKKIQYTETDLHLYSVLWNLRHAHDLFSVKLERETKFGKAFS
                     VFSRFDVCPSYGEVDITYLVVKAGIRKTLRGFAYVGGNGFSHSRHLMSTFMYLVREIL
                     VEHNWISETELYDQPISVYKFRAINFDKW"
     gene            complement(4928..5173)
                     /locus_tag="PXD94_001661"
     CDS             complement(4928..5173)
                     /locus_tag="PXD94_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6286507.1"
                     /translation="MLVHIRLSCIGDVLPTKLNEWTSEHQALQQEISHRALEAWQNRD
                     KDIKSVRFMQQMPSRQGLNFRFLSVSEQNGKLIVSRA"
     gene            complement(5245..5598)
                     /locus_tag="PXD94_001662"
     CDS             complement(5245..5598)
                     /locus_tag="PXD94_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286508.1"
                     /translation="MSQQTAGWDVIPFESSKTVKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFWTLLAAIIAF
                     VCGMAAVFGTQLSLI"
     gene            5972..6415
                     /locus_tag="PXD94_001663"
     CDS             5972..6415
                     /locus_tag="PXD94_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286509.1"
                     /translation="MKVSRFLGYCSIVTGVFSPLAQAQPNIIVYTTKMYPIQNSQLAS
                     QIYYLDQVENFEESISRNLSKSPEIAEQEMRAFFNSADWKAQEQQLKDAYQGVISGWQ
                     NGIKKVPAVFFDNPQSSPSVIYGTTDVQKALDEWHKWVKQQGESK"
     gene            6412..7353
                     /locus_tag="PXD94_001664"
     CDS             6412..7353
                     /locus_tag="PXD94_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286510.1"
                     /translation="MKKTFLTILISASLAVPTTLYPQPARASLNTASIAASSASTSCL
                     EYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNHDGQTPWTELQALNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGNTGYYCKTQTTPFMPYFLSGLDFFA
                     WRENMPEMFYPEALTPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     SRTAQPHIYFPAAKTGGAGEWPPGPVEEGQARTHKWQILYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            7371..9383
                     /locus_tag="PXD94_001665"
     CDS             7371..9383
                     /locus_tag="PXD94_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6286511.1"
                     /translation="MKSHFNFKKSHLRITTALLSSVSGLSYADSITDKLEFSKSDSII
                     SDRVFYQIGGGMGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMVLQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSSQPDAKQAQKDLEDKKGKDGRVWIGGQKKGGENQ
                     EAINVVGDVVKAGFNLLNKRNALDTSTISGSSCDGNLCQTWDNPQTAADFLTKIIGEQ
                     SISTCKEDCGTKTSSKAGVGLSPEIEEEHVRTIVKLENVLNMDVPKAELLADLSSTTV
                     PVTRGLIESLRDDPDVEVLSQRLSAEIATSKIMEKMLLARRAVLAGMREPNVAQNADA
                     QVELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLD
                     KRVQGLENGNVALTDLEAGSNINLSRRNIALSIPNIPNVAALIPNLPNRGGSGVGGNF
                     PSLAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTIVKVTKDT
                     VITKEDAERDLARRTAIFANNVRKELGDSNWNALPPNAQAVLVSYAYNYGSLAKTKSV
                     LDAARRSAQSGDMNALATAIRNRQVDNNGINARRRNQEADYILGKN"
     gene            9404..9832
                     /locus_tag="PXD94_001666"
     CDS             9404..9832
                     /locus_tag="PXD94_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286512.1"
                     /translation="MRKLLGITVLTLCTTSAFATDKNVVFSCTSTEGKPLTVKRVGND
                     YEYSYDKTIFKNPIKKAVTNDGSIIARGSGFTTYALELKNDGLKYVVGFVQPNGNSKE
                     FIEPGATISRDNDQPSIGSVDCDPYKKIYYKFDVHLMNTL"
     gene            10048..>10426
                     /locus_tag="PXD94_001667"
     CDS             10048..>10426
                     /locus_tag="PXD94_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286513.1"
                     /translation="MDITTQNIELRYLSEQFYLDVAPHLKEILLKETRPYCVLLVRVK
                     NLDFALPLRSNLPEREGVGIKTIPNPQEYGKFKGIDFSKAILLTDKSYLKTDTVQLKN
                     KSELLNIQGNERRIVREFKKYVSE"
BASE COUNT         3301 a         2033 c         2003 g         3089 t
ORIGIN      
        1 ggcggctatt atttgttagt aaaatattag ctcattatca tcaatttcat ctaaaattga
       61 gatttgagat aaatcttcca ctatcactga tttttctata ttgttaccac agcctttctc
      121 agtagtgcta tttacaagtg gtgcaatttg ttgctccagt ttatgagacc aatttcccat
      181 caaatgagcc agcgtataca tttgaatttt tgtttcattc aatagcgtat caccaactac
      241 aatacaagca ggaatatgta ataacgatag ctggatataa cacatcatgg cagctgtaaa
      301 atcaacatct tgacaatgaa cccaaagttg ttgttgggga ttgtatttcg catcaaacat
      361 cgcattaaca caaccaatca ccattacacc cgaaccacaa gttggttctg ataaagtaac
      421 aaatccctgt tgtgagatga ttctatcaca gtcctgtagt gtaactttcg ccattagatc
      481 tgcgatgtga gatggtgtaa agtattgccc tctataacca tcaccaagat tgagattcat
      541 aaatactgag cctaagaaat catagcgttt ttgtgttaaa gcattcaccg taacagctaa
      601 caattctgct aattgagtaa actcagaatc tgaatatcgt gccttagcat gagcaaagcg
      661 ttctcgcaaa ttaggttctg cttgatctcg atacgttact aggtacatat caagtgcaga
      721 gagtgtaata aaatcataaa acacttctga acgtcgataa tgtggtacga tacgttgaaa
      781 taagctgata aattgctttt caaagttttc cattagacta ctccttaaag tttacggagt
      841 aatcccccta gggaattacc cctagggtgg aaaaattaat taaggctatt caagtttctc
      901 ttcgaactca tcttcaaatt cttcattaaa gacttcctca gcaaaactgc gaatacccct
      961 atcatctatg taaatattgc agtatgagcc atcaagattt gcgatatatt tgcccgcaaa
     1021 ccacattccc ggctcaaaaa atgaaagtat aaactcaatg tcaggaaagg cagtacaaag
     1081 tgcttggaac caaccatcag gtggtcccca tggcgtatca aataggcaat taatagatcc
     1141 attatcatta aagctaactt gacagctatc tgaacataca ttccatttac agccccactg
     1201 ttcatttgac cagtcatacc aatctttgta accatgttga gctaggttag acagatattg
     1261 ctggcctaat gcgaggttta gccataatat actttgttgt tcaggacgtt ttatcaacca
     1321 gttgataaaa tgacctacat cccagcgatg atgctttgcc ttagcatata tccgatcaat
     1381 ttcggaccca tagatatact ttttgagtag agggcgtgtt aaacgtctat ttggtgaaat
     1441 ttttagaaat tccattgctc tatcaccaag actacttgat tcaatgcaga ttgattctgg
     1501 cattggaacg agtaattcaa agtctaaaca ccatttatca ctttcagttt ttctgaaaag
     1561 cttaggaata atttgtttag cttggataca atcgcaaaaa atttgtagtt tattttcaca
     1621 ccagtttggc ataatgtact cctaaataaa gtaggggtac acttgccttg aggcaatgta
     1681 ccccaagggt taaaagataa tcgatacata attcctaaag gattatgtat tactatgaaa
     1741 gattgtgttg aaagtacctt tcagcaaaga gttaagctgc ttcttcaaag acctcgtttt
     1801 tatcagccag ctgtgagata tttaatgcat ctaacatata ttgacaagct tcacgagctt
     1861 taccagaagc ccggacaata gcccgtttat cttcttttat aactcttatc caactttcaa
     1921 tatatgccgc attttgaggt accgtatcaa atccaaggta ggcacacaaa aatgctcccc
     1981 ccatttcggc aaccaactcc tcaaaagcat agactttgtt tccaaatttt gcttttccgg
     2041 aggtaatacc ctcacgatcg agccttttag cagcaccagt tgcatgagtc atttcgtgca
     2101 ataacacact atgaaacgct tgagcatttt caaactgttt tatttccggc attacaacta
     2161 aatctgcact gggttgataa tatgcccgat tactcggttt catttccacg tgtaaatcca
     2221 ttcctttgat aatctgccga atatcagcaa aaatctcgta ttggtttgga tccgatgtag
     2281 gctgtttagg ttctgatata ggatcgtaca gttcctcggg taaattttca cactggtcta
     2341 gattgaacag attgtgcttc ttaatgaaaa agtgttgttc aatttctgga ttaccttcct
     2401 cgtcatacac aatatttccc tcctcatcaa gtttttctct cttatcagga cggtagttca
     2461 ttacaacagt agctttttca cctttacgga tatatccgcc gagctcgtta gctttttttg
     2521 ctgtaatcca cttactctgt ttgtaaccgt attctgcggt agcaatccag agtaatagtg
     2581 tgttgatacc agaatacaat cggttagaga ccgcattacg aggtaatgcc atattacatt
     2641 ctggatgact ccacggtttt aaccatggtg cagtcccctg ttccagtgct gcaacaattc
     2701 ggtctgtgat atgttggtat aaatcaaatt tagtttgagt gttcattagt gtttctccaa
     2761 agttcaggta gaaaatgggg aaacactatt acccttatcg ggcactagtt tccccgatgg
     2821 gtggtttaaa atctcaacag agatagtttt tctaggttca tcaaaaccag tagaactgcc
     2881 tataatgcag taactacaga taatttgtta atcagtattg attcaagtta aacataatcc
     2941 tcatatcgaa aattactttg gtaatacaaa gttacattat ttcagatcca ttaaagtaaa
     3001 cgttgaaagc tattagcagc aataaagtga taactttttt ccgcagcttc tgtttccgta
     3061 atcattccct gctccagcaa aacatgggtg attccattga aaaactgatg taatagaaat
     3121 gaaataaaac ctgttgtttc aacttttact gggcgacaat gaaactgcat atcctgccaa
     3181 agtttaatat catcttttaa ctgttgagag ctcacaattt catatacaac agcagaaata
     3241 ttaactaaat taccgttgtt attcagccaa attgtataac gattccaatc tccttttctc
     3301 attggctcta tacaaatgtg aaataggtat tggtaggctt tattatttaa attgcggtta
     3361 tcccagatgt atttgagtaa atctaaatcg tgtttcataa tgtttctcca aaagaaaaaa
     3421 gtggagaaac atcttaccta tagggaaaat atttccccac agggtaaaaa acaatctcga
     3481 taagcggtca aattgaaata aaaaacctac aaatcaggca tgcaagtagg caaaacgtgc
     3541 ggcattacta cgagcagaag ctatagcatt actaataagc tcttgtcgat aaccactcaa
     3601 tgaatggtct gtcggcgaat agcttacgcc tgtaatttca tcgctaccta gttgaatgcc
     3661 actttcctta tcagtaacgg ttgctacaaa atcagcatag caaagtttcc cttgtaggat
     3721 ttcctctatc attgcctcaa aatcgcagac atcaccacat agccaatcaa cagactctga
     3781 ataaaattca gaggatttac gtttaagttc tatcagatag ttttccgtgt taaatttgaa
     3841 aatagtttcc gtctgatacg gtgaggcctc aataatctga tagccattcg attctaaggc
     3901 acaggaagta tctttaatat actgacctaa atcttgaata aatccatccc acagggcttt
     3961 gtatttttcc acttttgatg tagggaaata ccactctttt cgggcatcac gatcatagaa
     4021 aaatgccaaa tcatctgctg ttctaacaga aagctccatc gtattacaat gcgaatagcg
     4081 gtacccaaaa ctatttcgta tgatttcaaa atccacgcca tagtgaggct cccaagattc
     4141 aatgtgattc agcagattag aaaacatttt ttgtgtgcgt ttctgaggat ttagcttgtt
     4201 gaaaagatta attaacgtct ccgtattaag cctaccgtag aatgctacgc catcgccttg
     4261 acaatgagaa agtgaatact caatgtttaa gcaattagga aaaccagctt taacaagagc
     4321 gtgtttaaaa taagtgttta agactgagcc agacattgcg cttctcctaa aattagaggg
     4381 aaacacatta cccttttccg gaaattgtgt ttcccgaaag ggtgaaagat tgattaccac
     4441 ttatcgaagt taatcgctct gaatttataa acggatatag gttgatcata gagttctgtt
     4501 tccgaaatcc aattgtgttc aaccaagatt tctctgacaa gatacataaa tgtactcatc
     4561 aaatgacgac tatgactaaa cccgtttcca ccaacataag caaaccctct cagggttttg
     4621 cgaattcctg ctttaacaac aaggtaagta atatcaacct cgccataaga cgggcagaca
     4681 tcaaagcgag aaaaaacaga aaatgctttg ccaaacttgg tctcacgctc taatttcaca
     4741 ctgaatagat catgtgcatg gcgtaaattc caaagtacac tgtataagtg caaatctgtt
     4801 tcggtatatt gaattttttt acgagccata atgtttctcc taaaaaataa aagggaaaca
     4861 ttacttccct ctggggataa tgcctcccct tgtgaggtat aagaatagga tgtgaatatg
     4921 aagttgccta tgctcgacta acaattagct tgccgttctg ctcggacaca ctcagaaagc
     4981 gaaaattaag tccttgccga gacggcattt gttgcataaa tcgcacgctt ttgatgtcct
     5041 tatctcgatt ttgccacgct tctaaagctc tatgactaat ttcttgctgt aaggcttgat
     5101 gctctgatgt ccattcatta agtttagttg gtaaaacatc tccaatacag gaaagtctaa
     5161 tatgaactaa catacatttc tccagaataa ggggaaatgc attcccctta agggaaacaa
     5221 catttccccg atgggtgagt taaattagat taaagagagc tgagtaccaa aaacggcagc
     5281 cataccacat acaaaggcaa taatggcagc caacaaagtc cagaaaaaca tcttacgctt
     5341 aatgcgggaa atttcctgtt catattcggc tgcttgttcg tctaagcaca tttgcagttc
     5401 taaggctcgc atttcatctt cttcagcaat ccgttgattg atgcctttgg tgacttcaac
     5461 atacttgctt ttggctttat gtttcgctga aacaaaaata ttacctagct tgttgccaag
     5521 tttccaaaaa agagaaggtt ttacggtttt ggatgattca aacgggataa catcccatcc
     5581 tgcggtttgt tgactcatag cgagttctcc taaaagttaa gtgaataaaa ttcctcagga
     5641 gatcgcaatt ccaagggaat tgaaatatcc ctcggaaggt aggtgtgtag gtagcattcc
     5701 tgcaaatttg cagagttccc tttcagaatg ctgggagaca ttttgggtaa ataactcaac
     5761 tacctctaag gatagccgcc gcttttcaag agttatgcga cttttaaggg ctgtatctac
     5821 agcgtcttaa tgcttaatat catacgaatt cctccaataa aatcaaccgt ttttttgttc
     5881 aaaaaatatc tcttttttga aaagagtttt tcctatcctc aacataaaaa tctcgctaat
     5941 tttctgctta tttgttcggg aggagagcgt gatgaaagta agtagatttc tgggatattg
     6001 cagtatagta acaggtgtgt tttcgcccct tgcacaagca caacccaata tcatcgtcta
     6061 taccacaaag atgtacccta ttcaaaattc acaacttgct tcccagattt attatcttga
     6121 ccaagtagag aattttgaag agtcaatcag tcgaaacctc agtaaatcac cagagattgc
     6181 cgaacaagaa atgcgtgcat tctttaattc agcagattgg aaagcccaag agcaacaatt
     6241 aaaagacgct tatcaaggcg tgatcagtgg atggcaaaat ggcattaaga aagtacctgc
     6301 ggtgtttttt gataatccac aatcttctcc gagtgtgatt tatggtacga ctgacgtaca
     6361 gaaagcatta gatgaatggc ataaatgggt taaacaacaa ggtgaatcaa aatgaaaaaa
     6421 acgtttctta cgatactgat ctctgccagt ttagctgtcc caacaacctt atatcctcaa
     6481 ccagccagag cctcattgaa taccgcttcc attgctgcat caagtgcctc aacaagctgt
     6541 ttggaatata aagttgttgg aacctgtttt tggttatttt gtacctactt tggatgtaag
     6601 gttcgcacat caaccaaaat taagcattat ttacctgaaa tggttgtttc atcttataac
     6661 cacgatgggc aaacgccatg gacagaatta caagctctca atcctggtgt aaaaggtgga
     6721 gaataccgtt ctccacaacg ccaatactct caactcactt tcaaaaatgc ggatgcgttt
     6781 ggacacccac aaggagctat cggtcaatta ttaggaaata cggggtacta ttgtaaaact
     6841 caaactacgc catttatgcc ttactttttg agtgggctgg atttttttgc ctggcgtgaa
     6901 aatatgccgg aaatgtttta cccggaagct ctgactccgg gaatgcggga agttggccgt
     6961 gtaggcgata gctggggaaa tatttaccca cgttccggtt gggtaaccca agcacacgat
     7021 tataaagcct ctgcgaccat tgtgcagaga gtggcagaca ttgtgagccg aacagctcag
     7081 ccacacattt atttcccagc agccaaaaca ggtggtgcag gagaatggcc tccaggaccg
     7141 gtcgaagagg gacaagcaag aacacataaa tggcaaatac tttaccctca aatgtcaaat
     7201 acctgctctg tttttcctaa tggagggcaa acagatacct attcttctca aatttcaaat
     7261 caaggtgatt atgcttgggc attatggcga ccttatagtt gttgcaaacg ccgtgggcaa
     7321 acattccttt acagtattga ttggtcacac taataacatt gggagcaata atgaaatctc
     7381 actttaactt caaaaaatcc cacttacgga tcacgacagc attattatca agtgtatcag
     7441 gactatctta tgccgattcg attacggata aactggaatt tagtaagtcg gattcgatta
     7501 tttcagaccg tgttttttac caaatcggcg gtggaatggg ttatatggct ccacctagtc
     7561 gtggtaatac caaagttgtt gatttaggaa ttggttggaa agcgaattta acctgtggta
     7621 attttgatat taaaacgacc attaaaaatg atttaaacgg tctcaaagac ggctttaaag
     7681 atttaatggg gaatgtcatt gattcagcga aaggtgcggt tgcaagtatg cctgcaatgg
     7741 tattgcaacg ggccaaccct caactctacg atttactcac aaatggtatg taccaaggta
     7801 agctggattt caaccgtctt aaaaccagtt gtgaagaaat gtccaaagtt ttagcagatc
     7861 atacactggg aggaaaatgg gggcaagcag ctgatttaga aagctataag caaattacct
     7921 cctctcaacc tgatgcaaaa caagctcaaa aagacctgga agataaaaaa gggaaagatg
     7981 ggcgagtttg gataggaggt cagaaaaaag gtggggaaaa ccaagaagct atcaatgttg
     8041 ttggcgatgt tgtcaaagct gggtttaatt tactgaacaa acgtaatgcc ttagatacca
     8101 gtacaatttc aggttcaagc tgtgatggga atctttgcca aacttgggat aatccacaaa
     8161 cagcagccga ttttttaact aagattattg gtgaacaatc tatttcgact tgtaaagagg
     8221 attgtggcac taaaaccagc tcaaaagccg gtgtaggctt gagtccggaa attgaggaag
     8281 aacatgttcg tactattgtc aaattagaga atgtactgaa tatggatgtg cctaaggctg
     8341 aattgttagc tgatttgagt tcaactactg taccagtaac aagagggctt attgagagtc
     8401 ttcgagatga tcctgatgtt gaggtattaa gccaacgctt atctgcagaa attgcgacta
     8461 gtaagataat ggaaaaaatg ctcctggctc ggcgagcagt cttagctggt atgcgtgagc
     8521 caaatgtcgc tcaaaatgcg gatgcacaag tagaactgga aagagtactc aatattattg
     8581 accgtgaaat ttcccaagta aaactagaaa tggatatgca aaaaatgatc accggcaata
     8641 cggcttcggt gattttgcaa agcaaatccg aacgagaagc tcttgctggt gctcatagtt
     8701 tatctcaaga cagtctggat aaacgtgttc aaggacttga aaacggtaat gttgcattaa
     8761 ctgatttaga ggcaggctct aatattaacc tctctcgtag aaatattgca ttgagtatcc
     8821 cgaatattcc gaatgtagct gctctcatac ctaatctgcc aaatcgaggt ggttcaggtg
     8881 taggtggaaa tttcccatca ctcgcacaaa tcagcggttc aacgatggat caagcaacag
     8941 gattactacg taattttgaa ggctttatct ctaaggctca atgggatgta aatgctcatc
     9001 gagtaggata tggttccgat acaattaccc gagctgatgg tacgatcgta aaagtaacca
     9061 aagatacggt cattaccaaa gaagatgcag agcgtgattt ggcccgccga acagctatct
     9121 tcgcaaataa tgtgagaaaa gagcttggag attcaaattg gaatgcgtta ccgccgaacg
     9181 ctcaagctgt tttagtgtct tatgcatata attatggttc acttgctaaa acaaaaagcg
     9241 tacttgatgc agccagaaga tcagctcaaa gtggtgatat gaatgcttta gctactgcaa
     9301 tcagaaatcg ccaagttgat aataatggca tcaatgcacg tcgccggaat caggaagctg
     9361 actatattct tggtaaaaat taacctcata aggagttaca tttatgcgta aattattagg
     9421 tattactgta cttacacttt gtacaacttc agcatttgca acggataaaa atgtggtatt
     9481 ttcttgtacc agcacagaag gtaaacctct cacagttaaa cgggtaggaa atgattacga
     9541 gtattcgtat gataaaacta tcttcaaaaa cccaatcaaa aaagcggtga ccaacgatgg
     9601 ctcaattatc gctagaggat caggttttac cacgtatgca ttagaactga aaaatgatgg
     9661 tttgaagtat gtagttgggt ttgttcaacc aaatggtaac tcaaaagagt ttattgagcc
     9721 tggagcaacg attagccgtg ataatgatca accaagcatt ggctctgtag attgtgatcc
     9781 ttacaaaaaa atctattata agtttgatgt tcatttaatg aataccttgt aatgcgataa
     9841 ttcaataata tgggggaatg gcttgatttt tcatccccca tactctatta tatagatgtg
     9901 gtgctagagc cagcccacgt taaaaaatac aaagctcggt agatgaacta ttgtggtgcc
     9961 agagccggcc cacgttaaaa aatacaaagc tcggtagatg aggcccctta agtcgtaaga
    10021 tttaaggggt tttgcttagg atataaaatg gatattacaa cacaaaatat tgaacttaga
    10081 tacttatctg agcagtttta ccttgatgtc gctccacatc tgaaagaaat tttgcttaaa
    10141 gagactcgcc catactgtgt tctattagtt agagttaaaa atttagattt tgccctacca
    10201 ttgcgttcta atttacctga aagagaagga gttggaatta aaacgatacc taatcctcag
    10261 gaatacggca aatttaaagg aattgatttc tcaaaagcca ttctactgac agataaaagc
    10321 tatttaaaaa cagataccgt tcagctcaaa aacaaatcag aattgttgaa tatccaaggt
    10381 aatgagcgta gaattgttcg tgaatttaag aaatatgtga gtgaat
//
LOCUS       DANLXQ010000087        10276 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.087, whole genome shotgun sequence.
ACCESSION   DANLXQ010000087 DANLXQ010000000
VERSION     DANLXQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.087"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            142..1119
                     /gene="manX"
                     /locus_tag="PXD94_001668"
     CDS             142..1119
                     /gene="manX"
                     /locus_tag="PXD94_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL6286514.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHSVAVDQNDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
     gene            1131..1931
                     /locus_tag="PXD94_001669"
     CDS             1131..1931
                     /locus_tag="PXD94_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL6286515.1"
                     /translation="MELSALQIVLLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            1945..2781
                     /locus_tag="PXD94_001670"
     CDS             1945..2781
                     /locus_tag="PXD94_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL6286516.1"
                     /translation="MTTEIKKVTKSDLNAVVRRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNGPGRKEAIKRHSEFFNTQPFVGAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTVRPVFAALGAGLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTIEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGAATGA
                     LA"
     gene            2874..3359
                     /locus_tag="PXD94_001671"
     CDS             2874..3359
                     /locus_tag="PXD94_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL6286517.1"
                     /translation="MTNTILILFILLGLVFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRSPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLAYVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(3445..4236)
                     /gene="tpiA"
                     /locus_tag="PXD94_001672"
     CDS             complement(3445..4236)
                     /gene="tpiA"
                     /locus_tag="PXD94_001672"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL6286518.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGVSLKAPAFAVIVKAAAAAKA"
     gene            4396..5310
                     /locus_tag="PXD94_001673"
     CDS             4396..5310
                     /locus_tag="PXD94_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL6286519.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTNASTLFVGEKLAKFVGISEREMSYAGLKDRHNRKIRVGSLAGNHFELLLRDVAES
                     EELIYRLQQLQAVGFPNYFTEQRFGRDGHNLTQALRWANGEISVKDRKKRSFYLSAAR
                     SEVFNLVVSQRINDCLTQKILENDYVQLAGSNSFFCVKSDEIAETQARLAQQDLLLTA
                     PLIGERSLELECNPAEKAIIEQHAQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGL
                     RLKFFLDSGSYATALVRELILLTEESEA"
     gene            5481..6269
                     /gene="surE"
                     /locus_tag="PXD94_001674"
     CDS             5481..6269
                     /gene="surE"
                     /locus_tag="PXD94_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL6286520.1"
                     /translation="MRILISNDDGFHAVGIQTLAKALRDAGHIVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     TQRFPSNHVLNVNVPNLPYSELKGTMITRLGERSPAAEIVKQKDPRNADVYWIGVTGE
                     PLDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            6256..6840
                     /locus_tag="PXD94_001675"
     CDS             6256..6840
                     /locus_tag="PXD94_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL6286521.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTIVLAVVAYIIYTLMK"
     gene            6858..7358
                     /locus_tag="PXD94_001676"
     CDS             6858..7358
                     /locus_tag="PXD94_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL6286522.1"
                     /translation="MKKSLFVLSLISFALVGCSSNSGESTSAVETTEANATWQSADSI
                     QTAPMPASMNQPIATQPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVR
                     DANNAPIYSQITKGCYTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVG
                     QVLRVR"
     gene            7371..7571
                     /locus_tag="PXD94_001677"
     CDS             7371..7571
                     /locus_tag="PXD94_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286523.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            7590..8870
                     /locus_tag="PXD94_001678"
     CDS             7590..8870
                     /locus_tag="PXD94_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL6286524.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATETITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRAIDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            9111..9302
                     /locus_tag="PXD94_001679"
     CDS             9111..9302
                     /locus_tag="PXD94_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286525.1"
                     /translation="MDPTEPSHRIYILLSEDRFQAEVFSTKLSESSMLLEAVKGGYIS
                     KDGKVRLLKKAEGWKIYYE"
     gene            9295..9861
                     /locus_tag="PXD94_001680"
     CDS             9295..9861
                     /locus_tag="PXD94_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL6286526.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVPAHTETSKAMSKALKKRGFVF
                     VGETTCYAFMQSMGLVNDHLNNCVFKHK"
     gene            complement(9908..10138)
                     /gene="acpP"
                     /locus_tag="PXD94_001681"
     CDS             complement(9908..10138)
                     /gene="acpP"
                     /locus_tag="PXD94_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL6286527.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
BASE COUNT         3008 a         2099 c         2179 g         2990 t
ORIGIN      
        1 ttagcccaat ttatagccaa aaaagcttat tgtctgtgat atagatcaca cttttgtaat
       61 taatacattg tgtgaaataa ttatcatatt aaaatctcgc cccagtaaat aaacaactta
      121 aaaaaccgca aggagtaaca aatgagcatt gctattatca tagcaaccca tggtgttgct
      181 gcagagcaac ttctcaaaac aactgagatg ttgattggtg aacaagaaga tgttgcttac
      241 attgactttg ttcccggcga aaatgcagaa acgattatgg gcaaatatca agcattggtt
      301 gaaggtccac tatcacactg tgatcaagtg ctgtttttgg tggatatgtg gggaggaagc
      361 ccattcaacg ccgcaaaccg attccaagat ggaaaatcgg gtatggatgt tgttgcgggt
      421 gtgaatattc ctatgttagt gaacacctgt atggctcgtg atgatgtgga gtcgttggaa
      481 gaattggtgg aagtcgcatt agaagccggc tcaaccggga ttcgttcact taatcaccaa
      541 cctgaagtgg ctgaggtgca aaccgctact cctgcacaag aaactgcagc tgttcaacag
      601 cctgaaattg ttgatgacgg acgtcgtatg accattgccc ttgcccgtat tgatgaccgc
      661 ttgattcacg gtcaagttgc gacggtttgg acaaaagaaa gtaaagtcaa tcgcattatt
      721 gtggtaaacg aaggtgtagc aaaagatagt gtacgaacta ctatgctaaa aagtgttgct
      781 cctcttggcg taacggctca cgttgttgat gtacctaaaa tgattcgtgt ctataacaac
      841 cctgaatatg ccgatgatcg tgtaatgtta ctctttacca acccaagtga tgtcttagcc
      901 ttaattgaag gcggtgtacc actcaatagc attaacgtgg gcggtatttc gcataaagaa
      961 gggaaacaaa tggttagcca ttctgtggcg gttgatcaaa atgatattga tgccttcaaa
     1021 gcaattgatg cgaaaggcgt tgaattagaa gtacgcaaag tctcaagcga tagccgtcag
     1081 tatatgatgg atttattgaa aaaacataac ttaatctaaa ggaaattact atggaacttt
     1141 cagcattaca aattgtgctt ttattcctcg ttgcctgtat ctcaggtatg ggctcaattc
     1201 ttgatgaatg gcaaacccac cgtccgttaa ttgcctgtac gatgataggc ttagtgcttg
     1261 gtgatgtgac taccggcgtt attgttggtg gctcattaga gttattagct ttaggttgga
     1321 tgaacatcgg tgccgctgtt gcaccagatg cggcattagc ctctattgtc tcaaccattt
     1381 tagtcattgt aggtcgtcaa gatatttcaa ctggtatcgc catcgctatt ccgcttgcag
     1441 ctgccggtca agtattaacc tatgttgtgc gtgcaatcac cgtaggtttc caacacgcag
     1501 cagataaatc aattgaagac ggtaacttaa gccgtttaga ctggttacat cgtggtgctt
     1561 tattgcttca agcaatgcgt attgccattc cggcattaat tgtggcatta acagcaggta
     1621 cagatgcggt tcaaacaatg ttaaatgcca ttcctgaagt ggttacaacc gggctgaaaa
     1681 ttgccggtgg cttcatcgcc gtggtgggtt atgcgatggt gatcaatatg atgcgtgcag
     1741 gtcatttaat gccgttcttc ttcgcaggct ttgtaattgc aggctttacc gatttcaatc
     1801 ttgttgccct tggggtatta ggtacggtta ttgcaatggt ttatatccat atgcacccta
     1861 aatataacaa aagccaacag gtagtggtgg cagctgcacc aaaaaatgat ttagacaacc
     1921 gtttagacta agagggcatt taaaatgaca actgaaatca aaaaagtaac aaaaagcgat
     1981 ttgaatgccg ttgtacgtcg ttcaaactta ttccaaggtt cgtggaactt tgagcgtatg
     2041 caggctcttg gttttgcgta tgcgatggta ccggttatca aacgtttata tccggatcca
     2101 aatggcccgg gtcgcaaaga agcaattaaa cgccattctg agttctttaa cacccagcca
     2161 ttcgtcggtg caccgatttt aggggtaacc attgctatgg aagaagaacg tgccaacggt
     2221 aaagaaatcg acaatgccgc aattaacggt attaaagttg gtttaatggg accattagcc
     2281 ggtgtgggtg acccaatttt ctggggaact gttcgccctg tatttgccgc attaggagca
     2341 ggcttagcct taaaaggtga gcttttaggc ccaatcctgt ttttcgcctt atttaacata
     2401 gtgcgtttag ccgcacgcta ttatggcgta gtgtatggct ataaaaaagg gttagatgtt
     2461 gtacaagata tgagcggcgg tttattgcaa aaattaaccg aaggtgcttc aatcttaggt
     2521 ctgtttatta tgggggcatt ggtacaaaaa tggacgagca ttaacgttcc gcttgtgctt
     2581 tcaaccattc aaaaacaaga cggtaccatc gaagttacca cagtgcaaag cattctcgac
     2641 agcttaatgc cgggcctact cccattatta ttaacctttg cttgtatgtg gttacttcgt
     2701 aatcgagtaa atgctatctg gattatcctc ggtttctttg ttatcggtat cctcggcgct
     2761 gcaaccggtg cattagctta attgcaaaat tttctgtgaa aacgaccgct tgaacttgaa
     2821 cattcaagcg gttttttata cgctttagcc caaataaatt cacttgctta attatgacca
     2881 ataccatcct tattctcttt attttacttg gcttagtatt cgccatttat gaccaagttt
     2941 ttatgcacaa actcaaaggc acaaccttac tggaaatccg tttaaaaaag caaaaaacaa
     3001 ttgatacttg gttattgatt ggtttaatcg tcttgtcaat tagctatggc gtacaaaatc
     3061 aaatccagcc ttttacactt tacctgctgg cgacttgcat tattctgtcg gtctatctcg
     3121 cctttttccg ttcccctcgc ttgttgttaa aacagcacgg ttttttcttt gcgaatgtct
     3181 tttttaatta cgacaagatt tatcaagtca atattgcaga aggaaaagca gacgaaaaaa
     3241 ttttggtaat tgatttacat agcggaagaa ggctgcttgc ctatgttgaa aatgcggaag
     3301 atcttcaacc ggtagtggat ttttttggcg gttataaaaa ggaaagtgag cagaaatagt
     3361 ttgcgaacaa aacacaagcg gtcatttttc cctgaaattt tgcaaaaaac tgaggaaaaa
     3421 tgaccgcttg taaaatcggt taaattaggc tttagcagcc gctgccgctt taacgatcac
     3481 cgcaaaagca ggcgctttaa gtgaaacacc gcctactaat gcaccgtcaa tatccggttg
     3541 agtgaataac tctgctgcat tggcatcgtt tacagagcca ccgtattgaa tgatcacttg
     3601 gtcagccact gcttgcgatt tagctgcaat atggtcacga ataaatttat gcactgcttg
     3661 tgcttgtgcc ggagtcgctg atttgccggt accgattgcc caaattggtt cgtaagcaat
     3721 aactgcaccg ttgaatgctt ccacaccgtg cgcgttgatg accgcatcaa tttgtcttgc
     3781 acacacttct tcagttttac ctgcttcatt ttcggcttca gattcaccga tacataatac
     3841 aggcactaag ccggcatctt ttaatacttt gaatttttga gcaataaatt catcgctttc
     3901 tttgtggtaa gtacggcgtt ctgaatgccc gataataata tattttgccc caaactcttt
     3961 taacatttca ggtgaaatat caccggtgaa tgcccctttt acgttcacat caacgttttg
     4021 tgaaccaagt gcgattaagc ctttatcact gcaaccacag ccacaaccgg caagtgatgc
     4081 ttcagcgtgt gccaaataca tcaccggtgg agcgattgct acatcacagc cttcaacgcc
     4141 atgtaattct gcttttaaat ctgcaattaa ggttttagta aacgctttat caccgtttaa
     4201 tttccagtta cccattacaa gtggacgacg tgccattttg tttctcctat atgaagttga
     4261 acttaaatat aaacgttgca aactatacca aattttaatg tcgttttgtt gtttttattt
     4321 tgctttttct gatctagatc gaaaggttta acgtataatg ccgaaatctt attttttatg
     4381 ttattcgaga agtgaatgaa actgaattat ttatactctc ctcctcaagc tgttgggcga
     4441 ctaaaagccg aatatgcaga ctttattgtg cgtgaagaac tgggctacga actagccggc
     4501 gaaggggaat ttgtggcggt taaaatccgc aaaaccaacg ccagcacgct gtttgtgggg
     4561 gaaaagttag ccaaatttgt cggtatttcc gagcgtgaaa tgagttatgc aggcttaaag
     4621 gaccgccaca accgcaaaat tcgtgttggt tcgctcgccg gcaatcattt tgaattattg
     4681 ctacgtgatg tagcagaaag cgaagagttg atttaccgtt tgcaacagtt gcaagcggtc
     4741 ggatttccca attattttac cgaacaacgt ttcgggcgag acggacataa cctgactcaa
     4801 gccttgcgtt gggcaaacgg ggaaattagc gtgaaagatc gcaaaaaacg cagtttctac
     4861 ctttccgctg cccgctcgga agtgtttaat ttagtggttt cacagcggat taatgactgc
     4921 ttaacccaaa aaatcttgga gaatgattac gttcaacttg ccggctctaa cagctttttc
     4981 tgcgtgaaat cggacgaaat tgctgaaact caagcccgtt tagcacagca agatctactt
     5041 ctcaccgctc cgctgatagg cgaaagatcg cttgaattag aatgcaatcc tgctgaaaaa
     5101 gccatcatag agcaacacgc tcaattggtt gccttaatgc aaaaagagcg tatggcagcc
     5161 gctcggcgag caatgctttg taagccacaa aatctggaat ggcaatttga acaagaaggg
     5221 ctacggttaa aattcttttt agattcaggc agttatgcca ctgcactggt gcgagagtta
     5281 attctgctta ccgaagagag cgaagcttag tccataacac ataacaagcg gtaagatttt
     5341 aatcattttt tgcaatttgc aaaactttaa caaaatctta ccgctttttg cacttttaga
     5401 ctaaaaaaac tgtcgaaaaa ttgttagaat tgcgatcttt gtatttagag tctatcaaga
     5461 tgtaagatct aaaggaaatt atgcgaattt taattagtaa tgacgatggt tttcacgctg
     5521 tcggcattca aactttagcc aaagccttgc gagatgcagg ccatatcgtc accattattg
     5581 caccggatcg taaccgcagt gcagcttcaa gctgcctaac cttagtagat ccacttcggg
     5641 tgcatcgttt tgatgaatat aattatgcgg tgatcgccgg cacacctgcc gattgcgtgc
     5701 atttagcctt aaacggcttg tttgacggca aatttgcaga aaaatttgat ttggtggtct
     5761 ccggcattaa tcacggggca aatttaggcg atgatgtggt ttactccggt acggtagcgg
     5821 cagcattgga aggccgccac ttgccattac caagtattgc agtatcactg gttgggcgta
     5881 aacactcttc gtttttagac ggtgaaaatc attttggaac ggcagcacag gtagtgttag
     5941 atttattgga aaaaatggaa acgcaacgtt ttccatcaaa ccacgttctc aatgttaatg
     6001 tgccgaattt accttattca gagctaaaag gcacaatgat cacccgctta ggtgaacgtt
     6061 cacccgctgc ggaaattgtg aagcaaaaag atcctcgtaa tgctgatgtc tattggattg
     6121 gtgtaacagg tgaaccactg gatgaaagtt ttggtactga tttttatgca ttaaaccgag
     6181 attgcgtttc tatcacacca attcaagtgg atatgaccgc tcataaaacg atagcaacct
     6241 taaaaggcat ttttaatgaa actgtttgaa acaatttatg acaaaacaat gcagtggtct
     6301 aagcaccgct tcgctgcctt ttggttgagt tttgtcagtt ttatcgaggc gatctttttc
     6361 ccgattccgc cggatgtgat gttgattccg atgtcgatgg caaaaccgca aaatgcgttt
     6421 aaatatgcag tttacactac ccttgcttcg gttgccggtg gtgttattgg ttattttatt
     6481 ggcttatatg ctaccgattg ggtgcaaggc gtcatcgcaa attggggaat gcaagcaaac
     6541 tttgataaag caaaattgtg gtttgaaact tggggtgtcg cggtagtatt tttagccggt
     6601 ttttcgccga ttccctataa ggtatttacc atctgtgccg gtgtaatgca gatggcgttc
     6661 ttcccgtttg tgattactgc tgcgatttct cgctttgccc gttttatttt ggtagcaaaa
     6721 ctctctgctt ggggtggcaa aaaatatgaa caaaaaattc gccgctcaat tgaattaatt
     6781 ggttggggaa caatcgtttt agccgttgtt gcctatataa tttatacatt aatgaaataa
     6841 attttaagga tataaaaatg aaaaaatcac tttttgtttt atctctgatt tcttttgctt
     6901 tagtgggttg ttcaagcaat tcgggtgaat ccacttctgc ggtagaaaca acagaagcaa
     6961 atgcaacttg gcaatcagca gatagcattc aaactgcacc aatgcctgcc tcgatgaatc
     7021 aacctattgc cactcagcca acctatcagc agccgactta ctctgcacca caaccaattt
     7081 cggcagcacc agcaccaatg cctgtttcaa gtggtagtga tagctctcaa gttgaaacag
     7141 tagggaactg ccaagtggtg cgtgatgcga ataatgctcc tatctattca caaattacca
     7201 aaggctgcta cacagaaagt cactatacgg taggtaaaag tgatacgctt tatttaatcg
     7261 gttatctaac cggcaccaat gccaatcaaa ttgcgcagct aaatggttta aataccggct
     7321 ctccactgaa agtcgggcaa gtcttacgtg ttcgttaata ggcagagaaa atgaacagaa
     7381 cccttttagc atttgcaatt tcttgtgcat ttttgaccgc ttgtagctcg cctactcagc
     7441 cacaagttga agatccaaat atgttacctg caggcacatt acaaccgatt gaaggctcag
     7501 gcgcaagcca agacagttac agttggccgt ctgatattca atcaacgtca atgcctgaca
     7561 gtatgaaata attttaggac aatcatttaa tgaaaaaaca atttattctt ctccctttag
     7621 tctctgctgt tttggcagcc tgttcttcaa acaatccagc accggtggtg aatgcttccg
     7681 gtaataacga attaagcccg ggcgtgatgc agcctgttga tgtgagtgca ggtgcaatta
     7741 attcaagcgg tggctggcag tctgatattc aatctgcacc aatgccaagc tcgatgaata
     7801 ccactccggt gcaaacaatg ccccagccgg tttcagtgcc acaacctgtg gcgactgtac
     7861 cgcagccaat taccaatccg gtgattaccg aacaaccacc acagccgaca accacgacca
     7921 aaatcgtgaa aaaaacgaaa acggtcgaga aaaaagtgga tcaaaatttt gagatcccgc
     7981 gtgatgccaa caatgcccca atgtacaacc aaattcaaaa agggttctac gatggctcta
     8041 cctataccgt acgcaaaggc gacacaatgt ttttgattgc ctatattgtg ggtaaagatg
     8101 taaaagaaat cgccgctctt aataatatga gcgaacctta ccaattaagc gtagggcaaa
     8161 aactcaaaac cggcaaatct gcaacggaaa ctattaccgt ggaagaaaaa gtcaccgtac
     8221 cggttgaacc acaaattacc taccaacaag gtgcaaacgg cacaacctat gcttctggcg
     8281 gtaatattac tgggccggta aaagcaggca cagccaacgc tgatgtcacg ccggttaata
     8341 acggtattaa agcaacaact gcaactgctc cggcagtaac tgcttctgtc ggtacagtaa
     8401 atgcaacaga ggaccgtacc gcctccacca ttcgtgctat agatggcaca gcaagtggtg
     8461 ccgtacaaag tagtaatact gtttctgcac cagcttcatc aattaaatgg caatggccaa
     8521 ccagcgggcg tgtaatttca ggcttctcct cagcagaagg aggtaataaa ggcattgata
     8581 ttgccggcaa taaaggacaa gatgtacgtg ctgccgccgc aggtaaagtg gtatatgccg
     8641 gtaatgcctt gcagggttac ggtaacttaa tcatcatcaa acataccgat gatttcttaa
     8701 gtgcttatgc acacaacaat actattgaag ttgatgagca agaaacggtg aaagccggtc
     8761 agaaaatcgc gactttaggt agtacaggaa ccaatacgaa taaattacat tttgaaattc
     8821 gttacaaagg aaaatctgtc gatccggctc gttatttacc aagaaaataa gagtaacttt
     8881 ttagcataat ccgaccgctt gtgtactaca agcggtcaat ttttaggaaa attatgcaaa
     8941 tgaaaaaata tatcgcacac atgataagtt cgttactgct gatagcttgt tcggactacc
     9001 gagcaacacc aactagccca agcgatgctt acacttgctc tccttctttt cgctactctt
     9061 tcttaaaaaa aacgtgcata cctttattca gtatagccga cattcaatta ttggatccaa
     9121 ccgagccttc acatcgcatt tatattttgt tatctgaaga tcgatttcaa gccgaagtct
     9181 tctcaacgaa gctctctgaa tcttctatgt tattggaggc ggttaaagga ggctatattt
     9241 caaaagacgg aaaagttcgt ttactcaaaa aagctgaagg ttggaaaatt tactatgagt
     9301 gagactaaaa tacgttgtga ttggtgtaaa agcagtgaaa tttatatgaa atatcacgac
     9361 gaagaatggg gaaaaccgga gtttgacagc ctcaaactgt ttgagaaaat ctgccttgaa
     9421 gggcaacaag ccggactttc ttggattacg gtgttgaaaa aacgggaagc ctaccgccaa
     9481 gcattccatc agtttaatcc tgaaaaaatt gccaaaatgg gtgaagaaga aattgatttg
     9541 ttaatgcaaa acactagctt aatccaccac cgagcaaagt tagaagccat tataaaaaat
     9601 gcgaaagctt atatcacaat gcagcaaaat ggcgaagact tcagtcggtt tatttggtct
     9661 tttgtgaatt atcagccgca aattaatgat gtaccaacac ttgaagctgt tcctgctcat
     9721 acagaaacct caaaagcaat gtcaaaagcg ttgaaaaaac gtgggtttgt gtttgtaggc
     9781 gagactactt gctatgcttt tatgcagtca atggggctgg ttaatgacca tttgaataat
     9841 tgcgtgttca aacataaata aacacaaaaa attaggcgac catttggtcg cctaacatta
     9901 attcagatta ttgattgttt tgaacgtaat caattgctga ttgaactgta gtaatttttt
     9961 cagcttcttc atctggaatt tcgatatcga attcttcttc taaagccatt actaattcaa
    10021 cagtgtctaa agaatctgca cctaaatctt caatgaatga agcttctggt tttacatctt
    10081 ctgctttcgc gccaagttga tcaacaatga ttttttttac gcgttcttca atgctcattt
    10141 tgtttttcct atagtgaata tcgcataagt gcgagagtta aatagtgtaa tcgaaataag
    10201 tttaaataca accttttata aaaaggtcaa accagactat tcctatacac cagaatatat
    10261 ctgtttttcc attcta
//
LOCUS       DANLXQ010000088        10181 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.088, whole genome shotgun sequence.
ACCESSION   DANLXQ010000088 DANLXQ010000000
VERSION     DANLXQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.088"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..576
                     /locus_tag="PXD94_001682"
     CDS             <1..576
                     /locus_tag="PXD94_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL6286528.1"
                     /translation="GAYLEASEAQIFRGKYQEMEFVPNPDFDGPYYGLDFGFAQDPTA
                     AVKCWVFNGDLYIEFEAGKVGLELDDTADFIAKGVPQIADHVVRADSARPESISYLKR
                     HGLPRIIGVEKWKGSVEDGIEHIKSYGKVFIHPRCQQTLNEFRLYSYKTDRLSGDVLP
                     VVIDAHNHFIDALRYALTPLMQVKSAKGVLL"
     gene            573..1949
                     /locus_tag="PXD94_001683"
     CDS             573..1949
                     /locus_tag="PXD94_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL6286529.1"
                     /translation="MSLEQDRLAFLAEALGLGNIKRRTLWKEFGYPNTLTFNHFLKAY
                     KRNSIAFAAINRLLDGCWVDCPVIVEGEQKNESKQTTEWESKVERFMKRYWSAIKEAD
                     RRNLVGNYSALLLQVRDGQQWDQPILQGALSSIGEFGLVKLIPVWQSQLSVTEFQEDV
                     TADNYGEPLMYQFSESAFGKKSVSRNIKVHASRVILLNEGGDFNSPHSGVPLLEAGYN
                     KLVDLEKTSGGSAEGFLKNASRQLGIKLTKDVDLRQLEDSAKALGFSSFSDALNDKIK
                     KINSGTDSALITHEGDASVLSVAPADPKPTWEISANEFAASVQIPFTILFGQQTGRLA
                     SDEDKTDWANRCNGRRNGFLTDVITQLLNRLWFIGVLPMPKNSDVTVSWSDLLAPSEK
                     EKIANAQALASVATTSQSAFGFAAVTANEIRESLGFEPLPDNLIPPTIDDENEDQDEN
                     QATADSDK"
     gene            1903..2841
                     /locus_tag="PXD94_001684"
     CDS             1903..2841
                     /locus_tag="PXD94_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL6286530.1"
                     /translation="MKIKTKIKPLRIPTNKADPLNMGVSVAKLFRHIDVIYSQIKRSI
                     KQQILQKMSLRLQANNFEYFPSYLTATELADLLETLQKIIDKEILSNGSQGENLWFDS
                     YIDEAALKGTQSAVTDLGLQSEAYRSQRNLSSIVFSPAYFNRLAIAHMASYSEWQGLS
                     DELRKSLAGVITEAVLNGKNVKETAREIRQKLDVSSKRAKRIAQSSQLAAYRRAEWQE
                     AEEAKEELGLNTKLLHFSALKATTRLAHAQRHGKYFDVAEVREWYSRDGNQFNCYCKQ
                     SVVVVNADGKTDIEPLLVGLAKERKQWVGALKNVKK"
     gene            2828..4198
                     /locus_tag="PXD94_001685"
     CDS             2828..4198
                     /locus_tag="PXD94_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL6286531.1"
                     /translation="MSKNNVNIVTVINSKNISTESIDGDQHLIIRGVVPIVDDVVMND
                     GLYPADEINKSYNSLEGNFMPLGHPKIEGKYVSAQDVRAVNKHHVGAWAKNVRKESGK
                     VLVDMYINRRFAEGSEQGKTLLERLDGMVTNTNAEPIHVSTGLILNKVKQSGKSKGKK
                     YSWIATNMSFDHIAILLDVPGAATPDDGVGIFVNQDGSEMGVENVNLTQAADYRTEKL
                     FDKVRFYLMANSDLSFEEIHRALREAIRPEGKDKWSFWVEAVYPKYFIYSDDNSGKKY
                     KRSYFIDENDNVQLTSEPVEVVKKIGYEEITTNEEHNPMKTKILAVLNAANVKTEGLN
                     DDQLLEAYNKLQADKGKEPEKPKNEDDSEIDEKIKKAVNAAIEPLQQALQANTDKEQA
                     AMRAAVKAKFNMSDAAVNALQGEVLTELYAQTQQSVGLNNNSANGNGDNQWDGYSLNQ
                     EEKGNE"
     gene            4191..4625
                     /locus_tag="PXD94_001686"
     CDS             4191..4625
                     /locus_tag="PXD94_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286532.1"
                     /translation="MSNVIYRGSVKREPQTVNIVINDTSAPGAVVKLHNGKLEAAADL
                     KGRRFLLGNNRFKGQTIEQAYAKGDTATAFRLEPEQEYYAQIADGTYNFGDELTVKQA
                     GGKLTKAETGDVVLFFFDEESQRVISGGKGYADVVVANAYTK"
     gene            4640..5629
                     /locus_tag="PXD94_001687"
     CDS             4640..5629
                     /locus_tag="PXD94_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HDL6286533.1"
                     /translation="MLKFTKEQERFVINERINYDKKHALMAANSAGLLGNAMTLPKDV
                     WAEWDRDAVQIQREELVVFNDLAGISKSMPIGKLIHHFQTVSDSGSVNISLDGRSSAK
                     TDNVVLNYHGTPLPIIDSTFSFGWRDMSAAQSEGYQIDGAARANSLRKVAEKMEDLAL
                     NGDSSIVVGNAKLYGLRTAPNRMTNTHNLDLATATPKEIYDVFRDLISKFHAKNYYSP
                     VTLYVNYGDYFAMSTRDYSEQKSEGSILNKVMTIPQIAKIVPASRVPQNEILGLCKRS
                     DVYQVLNGMPLVTRPIARHNETDDYSFQIMAAVAVEFKFDDKGNAGYIQYTKS"
     gene            5641..5928
                     /locus_tag="PXD94_001688"
     CDS             5641..5928
                     /locus_tag="PXD94_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286534.1"
                     /translation="MKWLLTHDSHELKKGDVYEGETLPLWLVGKAIEIEEKSFEVATP
                     SDHADGETGETGETGETGETGETGETGETGETGETGETGETGQKGKKDKGK"
     gene            5931..6308
                     /locus_tag="PXD94_001689"
     CDS             5931..6308
                     /locus_tag="PXD94_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286535.1"
                     /translation="MAAEIELSEARTTLDELGFTSPNSLLELYVQQTNTLDQALNKSG
                     YSEETQKLIKLYLVAILAISSGARRIKSEGAPSGASRTFEYDPNALDNLKSAIRSIDP
                     KGCAESLLPKDKVVGFFDVVGGC"
     gene            6308..6652
                     /locus_tag="PXD94_001690"
     CDS             6308..6652
                     /locus_tag="PXD94_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286536.1"
                     /translation="MSNLSNWSYTAKATLWQAKGKNDDGVLVFSTPQIISCDYGADRK
                     RARFEIGREQAVKNVIWTEFAHAKLGDYVLIGESEQADPILAGAEEIIHIQRFADTFD
                     RNRDDFALITGG"
     gene            6654..7022
                     /locus_tag="PXD94_001691"
     CDS             6654..7022
                     /locus_tag="PXD94_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL6286537.1"
                     /translation="MGIKVKGIRQVKQRLNQVVGEVQSQKATRAMFRILNAVAPLAAH
                     YTPVDTSTLINSQFTEMEVNGTRLIGRVGYSANYAAYVHDPNIKQNFRKPSAKKEFLT
                     SALNESKATIKSIVKEELSL"
     gene            7019..7399
                     /locus_tag="PXD94_001692"
     CDS             7019..7399
                     /locus_tag="PXD94_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286538.1"
                     /translation="MISYVKAFKQWLEAHSLADGYVVQLYQWEDTQKAKPVIVIQPNS
                     GSPQVSDLSSEHYLLISLVAGKNSGYTIEERARAIMSKILVEPFASFGYIESMGGLPA
                     PIFTEDNRMIFRLPLRIISTNQEE"
     gene            7403..7885
                     /locus_tag="PXD94_001693"
     CDS             7403..7885
                     /locus_tag="PXD94_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6286539.1"
                     /translation="MGSPSPQNNAENLVVGRAVVLEYGTTDAKPQESEWKVAGAMTTK
                     SWDFSPNSVTSEADDAGGFPETLITNSDFSISGDGEWRKRPKSKELGIKDLVVLYANA
                     VKNRTQPYIWVRLKYAGLTILGKMVITALSSEAPTNDLVKFSIEFKVGDSSTIEIAAA
                     "
     gene            complement(7925..8341)
                     /locus_tag="PXD94_001694"
     CDS             complement(7925..8341)
                     /locus_tag="PXD94_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL6286540.1"
                     /translation="MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLL
                     SCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKVLLHNTMQEQAVSKAEIARLTNI
                     RPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG"
     gene            complement(8397..8573)
                     /locus_tag="PXD94_001695"
     CDS             complement(8397..8573)
                     /locus_tag="PXD94_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL6286541.1"
                     /translation="MKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELK
                     TGLVEGVKKQLGLK"
     gene            8669..9340
                     /locus_tag="PXD94_001696"
     CDS             8669..9340
                     /locus_tag="PXD94_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286542.1"
                     /translation="MKMVSYPITEIGEMLIATDGRDFLFRPSFKNIYKIGSPQEIVKI
                     YALLHGCEIREILPHILTKSESHQSYFWSVLNSPVFGRKILQNAMIVMSCCCDDDISL
                     LIGEWLPSKSGVRYKLGKMPPETIINFAKNLMGHGVIGCCELPKKEQSAKNEFSESFD
                     AVEYISLARTHFNMSREDAENLTMTELQQLLKSQMPAENNSYGQFTEEEYDRIMAEYE
                     KRRAA"
     gene            complement(9355..9588)
                     /locus_tag="PXD94_001697"
     CDS             complement(9355..9588)
                     /locus_tag="PXD94_001697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286543.1"
                     /translation="MKKDCTKQDFANWLNENQEVCWIKEVSIERILSCVDDYVAICNA
                     RSKDEFELLNDIRELFYSAHYKDFTPVLSGRFF"
     gene            9757..>10181
                     /locus_tag="PXD94_001698"
     CDS             9757..>10181
                     /locus_tag="PXD94_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286544.1"
                     /translation="MKKMIVFVASVLSSFVIASDWEIIEAAQNKVKAELIRKSEKKAE
                     LCLNVAQAVLITNQKVQKDTFSMCGEFSTEIVFSDSKAYSLAVCGKVKGKDIIGNPIE
                     SDYIYQRATKDFYFNYNGKKSAYYDPQSDLARLQEVSDS"
BASE COUNT         3047 a         2103 c         2418 g         2613 t
ORIGIN      
        1 ggtgcttatt tggaagcgag cgaagcgcaa atctttcgcg gtaaatatca agaaatggaa
       61 tttgtgccga accctgattt tgatggccca tattacggct tagattttgg tttcgcgcaa
      121 gatccgacgg cggcggtcaa atgctgggta tttaatggcg atctgtacat cgagtttgaa
      181 gcaggcaaag tgggcttaga acttgacgac accgcagact ttattgccaa aggcgtgcca
      241 caaattgccg atcacgttgt gcgtgcagat tcggcgcgtc ctgagtcaat tagttacctc
      301 aaacgtcacg gcttaccgcg cattatcggt gtggagaagt ggaaaggctc ggtggaagac
      361 ggcattgagc atattaagtc ctacggcaaa gtctttattc acccacgctg tcagcaaacg
      421 ctcaacgaat ttcgccttta ttcgtacaaa accgaccgct tatcgggcga tgtattgcct
      481 gtcgtgattg atgcccataa ccactttatt gatgcgctac gctacgcatt aacgccgttg
      541 atgcaagtta aaagtgccaa aggagtttta ctatgagcct agagcaagac agactggctt
      601 ttttagccga agccctaggg cttggcaata tcaaacgaag aacgttatgg aaggagtttg
      661 gctaccctaa taccttaaca ttcaaccatt tcttaaaagc ctataagcgc aatagtatcg
      721 cttttgcagc gattaaccgc ttacttgacg gttgttgggt tgactgcccc gtaatcgttg
      781 agggtgagca aaagaacgaa tcaaagcaga caaccgaatg ggagtccaaa gttgaacgtt
      841 ttatgaaacg ctactggtcg gcaatcaaag aggcagatag acgaaacctt gtcggtaact
      901 attcggcgtt attacttcaa gtaagagatg gtcaacaatg ggatcagcct attttgcaag
      961 gcgcgctatc tagcattgga gagttcggac tggttaaact gatccccgtg tggcagtcac
     1021 agctttcagt gacagagttt caagaggatg tgaccgccga taattacggc gaaccgctga
     1081 tgtatcaatt cagcgaatct gcctttggca aaaaaagcgt gtcacgaaat atcaaggttc
     1141 acgcaagtcg tgtaattttg ctcaatgagg gtggcgactt taattcccct cattctggag
     1201 ttcctctgct tgaggcgggt tacaacaagc tagttgatct cgaaaaaaca tcgggcggca
     1261 gcgccgaagg ttttctgaaa aacgcaagcc gacaattagg gatcaaatta acaaaagatg
     1321 ttgatttgag acagctcgaa gacagcgcaa aagcccttgg gttttcttca ttttctgatg
     1381 cgttgaacga caagatcaag aaaatcaact caggaacaga ctcagccttg attactcacg
     1441 aaggtgatgc ctctgtgttg tcagtcgcac cagcagatcc gaagccaacg tgggaaattt
     1501 ctgcaaatga gtttgccgcc tcagtacaaa tccctttcac gattttgttc ggacagcaaa
     1561 cagggcgatt ggcaagtgac gaggacaaaa ccgactgggc aaaccgttgc aacggtcgtc
     1621 gaaatggttt tctcaccgat gtgataacac aactacttaa tcgcttgtgg ttcatcggtg
     1681 tgctacctat gccaaaaaat agcgatgtca cagtgtcttg gtctgactta cttgcaccaa
     1741 gcgaaaaaga gaaaatcgca aacgcacagg ctttggcttc agttgcaact acatcgcagt
     1801 ctgcctttgg ttttgccgcg gtaacagcga acgaaatccg cgaatcttta gggtttgaac
     1861 ctctgcctga taatctgatt ccgccaacaa ttgacgacga aaatgaagat caagacgaaa
     1921 atcaagccac tgcggattcc gacaaataaa gccgatccgc tcaatatggg agtgtcggtt
     1981 gccaagctat ttcgtcacat tgatgtcatt tactcacaaa ttaagcgctc cattaagcaa
     2041 caaatcttgc aaaagatgtc actgcgactg caagcaaata attttgagta ttttccttcg
     2101 tatcttactg cgactgagtt ggcggatttg cttgaaacct tgcaaaagat tatcgacaaa
     2161 gagattttgt cgaatggttc tcaaggcgaa aatctctggt ttgactccta tatcgacgaa
     2221 gcagcactaa aaggcactca gtctgcggtc actgatttag gtctgcaatc agaggcttat
     2281 cgcagtcagc gaaacttatc atcaatcgtt ttcagtcctg cgtacttcaa ccgattggcg
     2341 attgcacaca tggcaagtta tagcgagtgg caaggtttaa gcgacgagtt gcgaaaatct
     2401 ttggcggggg tgatcacgga ggcggtttta aacggcaaaa acgtgaaaga aactgcccgt
     2461 gagattcggc aaaaacttga tgtgtcatca aaacgggcga agcgaatagc gcaatcaagc
     2521 cagttggcgg catatcgtcg ggcagaatgg caagaggcgg aagaggcaaa agaagaactt
     2581 gggctaaaca ccaagctctt acatttttca gcgctcaaag ctactacgcg attagcacac
     2641 gcccaacgcc acggcaaata cttcgatgtt gcagaggtgc gcgaatggta tagccgagac
     2701 ggcaaccaat tcaactgtta ctgcaagcag tctgtggttg tggtcaacgc cgacggtaaa
     2761 acagatattg agcctctcct tgttgggttg gctaaagaac gtaaacaatg ggtaggagca
     2821 ttaaaaaatg tcaaaaaata acgtgaacat cgtcaccgtc attaacagca aaaatatcag
     2881 cacagaatct attgacggag atcaacacct gattatccgc ggcgttgtgc cgattgttga
     2941 tgatgttgtg atgaacgacg gactttatcc tgcggatgaa atcaataaaa gctacaacag
     3001 cttagagggc aactttatgc cgcttgggca tccaaaaatc gaaggtaaat acgtttcagc
     3061 ccaagatgtt cgagcggtga acaaacatca cgtcggtgcg tgggcgaaga acgtacgcaa
     3121 ggaaagtggc aaagtgctgg tcgatatgta tatcaaccgc cgctttgcgg aaggttcaga
     3181 acagggcaaa accttgcttg aacgacttga cggaatggtg acaaacacca acgctgaacc
     3241 tattcacgtt tccacagggc tgattctaaa caaagtcaag caatcgggta aatccaaagg
     3301 caagaaatat agctggattg cgacaaatat gtcgttcgat catattgcaa tattgcttga
     3361 tgtgccgggc gcggcgacac ctgatgatgg cgtggggatt tttgtaaacc aagacggaag
     3421 cgaaatgggg gtggaaaatg taaacctaac acaagcggcg gattaccgca cagaaaagct
     3481 gtttgacaaa gtacgatttt acctgatggc gaactcagat ttatcgtttg aagaaattca
     3541 tcgcgcgctg cgtgaagcta ttcgccccga aggcaaagat aagtggtcat tttgggtcga
     3601 agctgtttac ccgaaatact tcatctattc ggacgataac agcggcaaga aatacaagcg
     3661 gagctatttt attgacgaaa atgacaatgt gcagctcact agcgaacctg tcgaagttgt
     3721 caagaagatt ggctacgaag aaattacaac caatgaggaa cataatccaa tgaaaaccaa
     3781 aatcttagcc gtgttgaatg cggcaaacgt gaaaacggaa ggcttaaatg acgatcagct
     3841 cttggaagcc tacaacaagc tgcaagcgga taagggtaaa gagcctgaaa agcctaaaaa
     3901 tgaagatgac agcgaaattg atgagaaaat caagaaagca gtcaatgccg cgattgagcc
     3961 actccagcaa gccttgcaag ctaatactga caaagagcaa gcagcaatgc gtgcggcggt
     4021 gaaagccaag ttcaatatga gcgatgccgc agtcaatgct ttgcaaggcg aggtattaac
     4081 tgagttgtat gcgcaaacgc aacaatcggt cggattaaac aataattcgg caaacggcaa
     4141 cggcgacaat caatgggatg gttactcact caaccaagag gagaaaggca atgagtaatg
     4201 taatttatcg cggttcggtc aagcgtgaac ctcaaacggt gaatatcgtg atcaacgata
     4261 cttcggcacc gggtgcagta gttaaactcc acaacggcaa attagaggcg gcggctgatt
     4321 taaaaggtcg ccgtttttta ttgggcaaca accgctttaa ggggcagaca atcgaacaag
     4381 cgtatgcgaa gggcgatact gcaacggcgt ttcgtttaga gcctgaacag gagtattacg
     4441 cgcaaattgc cgacggcact tataactttg gcgatgaact cacggtaaaa caagcgggcg
     4501 gcaaactgac caaagcggag acgggcgatg tagtgctatt tttcttcgac gaagaatcgc
     4561 agcgtgtaat cagtggcggc aaaggctatg ccgatgtcgt ggtggcaaac gcctacacta
     4621 aataagaggt aacaaagcaa tgttgaaatt cacaaaagaa caagaacgct tcgtgattaa
     4681 cgagcggatc aattacgaca aaaaacacgc gctaatggca gcaaattcgg cggggttatt
     4741 gggcaacgcg atgactttgc cgaaagatgt ctgggcggaa tgggatcgtg atgcagtgca
     4801 aattcagcgc gaagaattgg tggtgtttaa cgatttagcg ggcatttcaa aatcaatgcc
     4861 gattggcaaa cttattcacc atttccaaac ggtatctgat agcggttcgg ttaatatctc
     4921 attagacggt cgttcatcag cgaaaaccga taacgtggtg cttaactacc acggtacacc
     4981 gttgccgatt attgattcca ctttctcttt tggctggcgc gatatgtcgg cagctcaaag
     5041 cgaagggtat cagatcgacg gtgcagcacg cgcgaacagt ctccgcaagg tggctgaaaa
     5101 aatggaagat ctcgctttaa atggtgattc ctctattgtg gttggcaatg ccaagcttta
     5161 cggcttgcgt actgcgccaa atcgtatgac taacacacac aatctggatt tagccactgc
     5221 gacaccgaaa gaaatttacg atgtgttccg tgacttgatt agcaaattcc acgcgaagaa
     5281 ctactactct cctgtaacgc tttatgtgaa ctatggcgac tatttcgcaa tgagtacacg
     5341 tgattactcc gagcagaaat ccgaaggatc tatcttgaac aaagtgatga cgattccgca
     5401 aattgcgaaa atcgtgccag caagccgtgt accgcaaaat gaaattctcg gcttgtgcaa
     5461 gcgttcggat gtgtaccaag tgcttaacgg tatgccgtta gtgactcgtc cgattgcgcg
     5521 ccataatgaa accgatgatt actcgttcca aattatggca gccgttgccg tggagttcaa
     5581 attcgacgat aaaggcaatg ctggctatat ccagtacact aaatcctaag gcggtgcaaa
     5641 atgaagtggc tattaacaca cgatagccac gagttgaaga aaggcgatgt ttacgaaggc
     5701 gaaacattac cgctttggct tgtgggcaaa gctatcgaaa tcgaagaaaa atcctttgaa
     5761 gttgcaacgc cttctgatca cgctgacggc gaaactggcg aaactggcga aactggcgaa
     5821 actggcgaaa ctggcgaaac tggcgaaact ggcgaaactg gcgaaactgg cgaaactggc
     5881 gaaactggcg aaactggtca gaaaggcaaa aaagataaag gcaaataact atggcagcag
     5941 aaatagagct atccgaagcg cgaacaacgc ttgacgaact gggtttcact tcgccgaact
     6001 ctctgcttga gctttacgtt cagcaaacga acaccttaga ccaggcgttg aacaaatcgg
     6061 ggtacagcga agaaacacag aaactgatca agctctatct ggttgctatt cttgcaattt
     6121 catctggggc gcgccgtata aagtcagaag gtgcgcctag cggtgcaagc cgaacgtttg
     6181 agtatgatcc aaacgcgcta gacaacctta agtcggcgat tcgctcaatt gatccgaaag
     6241 gttgcgctga aagtttgcta cccaaagata aagttgtcgg ctttttcgat gtggtcggag
     6301 gctgctaatg tcaaatttat cgaattggtc ctacaccgca aaagccacgc tttggcaggc
     6361 taaaggaaag aacgatgacg gcgttttagt tttttctacg ccacaaatca tttcctgtga
     6421 ctacggtgca gatagaaagc gcgcacgttt tgagattggg cgtgaacaag cggtcaaaaa
     6481 tgtgatctgg acggagttcg cccacgccaa attaggcgat tatgttctga ttggcgaaag
     6541 cgagcaagcc gatccgattt tggctggcgc agaggaaatt attcatatac agcgctttgc
     6601 tgatacgttc gaccgcaatc gtgatgattt tgcgctgata acaggcgggt aaaatgggga
     6661 tcaaagtaaa aggcataaga caggtcaagc agcggctgaa tcaggttgtt ggcgaggttc
     6721 agagccaaaa agcaactcgg gcaatgtttc gcattttgaa tgcggttgcc cctcttgcgg
     6781 cgcactatac gcctgttgat acctcaacgt tgatcaacag tcaattcact gaaatggaag
     6841 tgaacggcac acgcttgatt ggaagagtgg gttattctgc taattatgcg gcttacgttc
     6901 acgatcctaa tatcaagcaa aacttccgca agccttcagc gaagaaagag tttttgactt
     6961 ccgcgctgaa cgaatcaaag gctacgatca aatccattgt taaagaggag ttgtcactat
     7021 gatttcttat gtcaaagcct tcaaacaatg gttagaggct cactctcttg cagacggcta
     7081 tgttgtgcag ctttaccagt gggaggatac gcaaaaagcc aagcctgtta ttgttattca
     7141 gccaaatagt ggttcgccgc aagtttctga cctgagcagc gaacactatc tgctaattag
     7201 ccttgttgca ggtaaaaatt caggttacac aatcgaagag cgagcaaggg cgattatgtc
     7261 aaagatttta gttgagcctt tcgcttcctt tggttacatc gaatcaatgg gcggattacc
     7321 cgccccaatt ttcaccgaag ataatcgaat gatttttcgc ttaccacttc ggattatttc
     7381 aacaaatcaa gaggagtagt ttatgggttc tccatctcca caaaataacg cagaaaatct
     7441 cgtggtgggg cgtgccgtag ttttagagta cggtacgact gatgcaaagc cacaagaaag
     7501 cgaatggaag gttgcgggcg caatgacgac aaagagttgg gattttagcc caaattcggt
     7561 tacatcagag gctgatgatg ctggcggctt ccctgaaacc ttaattacaa actcagactt
     7621 tagtatttct ggtgacggtg agtggcgcaa acgaccaaaa tcgaaagaac taggcatcaa
     7681 agatctagtt gtgttatacg caaatgcggt aaaaaatcgc acccaacctt acatttgggt
     7741 tcgtttgaaa tacgcaggct taacaattct cggaaaaatg gttatcactg cattaagtag
     7801 cgaagcccca accaatgact tagtgaaatt ctctatagag ttcaaggttg gcgattcaag
     7861 cactatcgaa attgctgcag cgtaaaaaat cccaccctct ataacaagag cggtgggatt
     7921 atcattatcc tagggatagt tgtaggggct ttcctaagct agccaaaata cgaccaatag
     7981 tgtcgatttt tgtaatatgg cgaggggcta aaattcgctg tacttctggc ggacggatat
     8041 tagttaatcg ggcaatttct gccttgctta ccgcttgttc ttgcattgta ttatgcaaaa
     8101 gcacttttgc gtagatgctc tctggcataa acacaggcac ttcgtcggta tttactgggc
     8161 gagtgagcgg aaagcgtcta tcttcatcga aataaatttc aacacaagat agtagcacgt
     8221 cttctgccat ttccattgct tcttcaaatg tatcgccttg ggtgatagct tctggcaaat
     8281 caggaaatgt cactacaaag cccccttctt cagcgggggt aaataacgct ggataaaaca
     8341 tattttctcc ttattcaagg gtgtagtgaa agccccttaa catcaggggc tttatattat
     8401 tttaagccta gttgcttttt aaccccttcc actaagcctg tttttaactc ttggcttggg
     8461 tgtttgggga ggtggcttct tttgcccttg tagtagagtt tcaaatgctt tgatccattt
     8521 tcaacctcta ccccattagc ctttagccac cttaggaatt cgctttgctt cactacctcc
     8581 tcctatttgt ttaacttggg ttcattatat gcaaaaatgc taactttgtc aacagaaagt
     8641 tagcaaaaat gctaattttt ttgttgaggt gaaaatggtt agctatccaa ttactgaaat
     8701 tggtgaaatg cttattgcaa ccgatgggcg agacttttta ttcagaccga gtttcaaaaa
     8761 tatctacaaa attggaagcc cacaagaaat tgtaaaaatc tatgcactat tgcacggctg
     8821 tgagattcga gagatattgc cgcacatttt aaccaaatcg gagtctcatc aatcttactt
     8881 ttggtcagtg ctgaattcgc ctgtttttgg cagaaaaatt ctgcaaaacg caatgatcgt
     8941 gatgtcgtgc tgttgcgatg atgatatttc cctgttgatt ggcgagtggt tgccgtcaaa
     9001 gtctggcgtt aggtacaagt tgggtaaaat gccacctgag acaattatca attttgcaaa
     9061 aaatttaatg ggtcacggag tgattggttg ctgcgaattg ccaaagaaag aacaatctgc
     9121 aaaaaatgaa ttttcagaaa gttttgatgc tgttgagtac ataagtttag cccgaactca
     9181 tttcaatatg agccgcgaag acgctgaaaa tctcacgatg accgaattgc agcaacttct
     9241 aaaaagtcaa atgcctgccg aaaataatag ctatggacag ttcaccgaag aagagtacga
     9301 cagaattatg gcggaatatg agaaaagaag agcggcttaa atagccgcta acatttaaaa
     9361 gaacctacct gatagaacgg gagtgaaatc cttgtaatgt gctgaataaa aaagctctct
     9421 tatgtcattg agtagctcaa attcatcttt ggatcttgcg ttgcagattg ccacatagtc
     9481 atctacgcag gataaaatac gttcaatact gacttctttt atccagcata cttcttggtt
     9541 ttcattcagc cagttggcaa agtcttgttt agtgcagtct tttttcataa gttctccttt
     9601 ttgattattt gcaaaaaaaa tcatatcaaa tcatccgttg ttatctattg gcgaaaataa
     9661 tttttgcgac acagatcgca aaaataggtt ttgacggtgg aaaaacgaat cttaattcgt
     9721 agaatggtaa aatttcttta tttttaaggt atttttatga aaaaaatgat tgtttttgta
     9781 gcttctgttt tgtcatcttt tgttattgca tcggattggg aaataataga agcggctcaa
     9841 aataaggtga aagcagagct tataagaaaa tcagaaaaaa aagcagagtt atgtttaaat
     9901 gttgctcagg ctgtcttaat tactaatcaa aaagtacaaa aagatacttt ctcaatgtgt
     9961 ggtgaatttt cgacagagat tgtcttttct gactctaaag catatagcct tgcagtatgt
    10021 ggaaaggtta aaggtaaaga tattatcgga aatccgatag agagcgacta tatataccaa
    10081 agagcaacta aggattttta ctttaattat aatggcaaaa aatcagctta ttatgatcct
    10141 caatctgatc ttgcaaggct tcaagaggtc tcagactcta t
//
LOCUS       DANLXQ010000089        10158 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.089, whole genome shotgun sequence.
ACCESSION   DANLXQ010000089 DANLXQ010000000
VERSION     DANLXQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.089"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            11..87
                     /locus_tag="PXD94_001699"
     tRNA            11..87
                     /locus_tag="PXD94_001699"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Met,seq:cat)
     gene            238..1698
                     /gene="xseA"
                     /locus_tag="PXD94_001700"
     CDS             238..1698
                     /gene="xseA"
                     /locus_tag="PXD94_001700"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL6286545.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHSQIPIISAVGHETDVTIADFVADLRAPTPSAAAELVSRDQNELKRQLQHQFDKV
                     NLAFDRVWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIYALVAAKKQ
                     VGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNITQRFERNP
                     LPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKILTRGYSITQ
                     TKDGKTLTSTENIERGEAIITQLKTGKIISQVVDFL"
     gene            2010..2095
                     /locus_tag="PXD94_001701"
     tRNA            2010..2095
                     /locus_tag="PXD94_001701"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2044..2046,aa:Leu,seq:caa)
     gene            2193..3017
                     /locus_tag="PXD94_001702"
     CDS             2193..3017
                     /locus_tag="PXD94_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL6286546.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLRTL
                     LIDTDTQPTLSSYYELEYQAPGGTYEFLHFKDVDPTHIISRTTIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETISQLCALLMPQFANSHFSA"
     gene            3029..4390
                     /gene="dnaB"
                     /locus_tag="PXD94_001703"
     CDS             3029..4390
                     /gene="dnaB"
                     /locus_tag="PXD94_001703"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL6286547.1"
                     /translation="MSSLNKSLVQPHSIEAEFSVIGGLLLDNELFDEVSSKINEGDFY
                     NFTHRLLFKHISELIEAGKPADILTLDQYLTQKKVIEDLGGFAYLAEIVKNTPSTANI
                     HAYTDIVLLYSKQRQLLKLGQFIVDQTQSAKTPDKFEAVVDDIEKKITEFSLSDNTKS
                     AADLSDIFANMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLKKFESAPVFYHSMEMPADQILQRFLAMRARVGLQNIRQADSLDDEE
                     WSKLTSAMGYILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPAAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMDCPVYALSQLNRSVEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEVYDADSPFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRTN
                     TTH"
     gene            4399..6045
                     /locus_tag="PXD94_001704"
     CDS             4399..6045
                     /locus_tag="PXD94_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL6286548.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPTHYSSDVEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIIADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLGKSLSSRELSSELENDGYIISHALILKMERCVNYLYPHIPEVLF
                     SGLGHTQIDKLLAIRNNAEEVWAAHQFDTDVTFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALEGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQT
                     AKSEPLIKNENKAVTPQKAQHIIDEVIDDNESDDEQNNAITDLSQLGSSLLDNQDNQE
                     DYLSQIAHNFGLTPGMSIQEQREQRAEANGLTFACEGRQPVEDIWLIHPARQYRSEAY
                     SLALDIAESMGIDHLVEHIVKQPVDYTYAMKPADTELSPSALTLYSFLSMLQTAEHKD
                     ENSCVLDSKFLFELDDLTLVKIFRLIRVSRHIRQQGGEND"
     gene            6038..6595
                     /locus_tag="PXD94_001705"
     CDS             6038..6595
                     /locus_tag="PXD94_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL6286549.1"
                     /translation="MIKPITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     IDEIMDISNSVVSFAKVEINHETFWKLLAIAQANTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQRYKQRMEILDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWYSKHK"
     gene            6754..7944
                     /locus_tag="PXD94_001706"
     CDS             6754..7944
                     /locus_tag="PXD94_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286550.1"
                     /translation="MFSSTNKPTEHGLLFFGNQHETVPTRLLQDPCLTPRAKFAWQLI
                     KCNVQAFQGGLFPSYETLSKMLSDRPYAEAKLSEKVVTQTLYLLRLTRWLTLCETVRD
                     ERGYVQGNVYILHDEPVPILDAIQLNSDYLSFLDKCTKHKDSLINGVANHIVESLITD
                     KTKWHYVSHLDCLQSRYQIYQQQQLVKDKQPSQTEQELSKIQQNILTSTMEVRDFDEE
                     VRKKSSKNQTSNKGVRAKNEDKSLILDLLPLGKSDVQYRTLYINKYCTGEPSEITWPQ
                     EIPLTSLEQKLTAQAMKGLDLGLCQAILLEVQQRIIQKQDVKKPKGYLYTLIQRAKQG
                     EFKPYYFELGKNLPSQNQANNNSAHSIQKLTEQPTEKPVLTSADREQMASRMQKFRAE
                     LLLQ"
     gene            8123..8881
                     /locus_tag="PXD94_001707"
     CDS             8123..8881
                     /locus_tag="PXD94_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286551.1"
                     /translation="MTTQLDPQLGPLRSEITFTLHTQYAHKLWVGRPMIRNEEGKVTQ
                     SSIISVPNCFAMLTQIQRAASEDDPYADDYLIQFEESVINYRKEIQKLTNDIVTLYAK
                     MLPEGISIERCANISPISYPIYVNSQLGYQLLYLLGDFDSLCRSVMTAAHIAIINRAE
                     AQDWIEAGARLIRKCFGIVERYKSSGITRRDYQEKNARYQAAIKRMGYTLSDAVLTGE
                     HRAEFAPFIKQNTTVEEEQSVETHITAQTNESQK"
     gene            8890..9375
                     /locus_tag="PXD94_001708"
     CDS             8890..9375
                     /locus_tag="PXD94_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HDL6286552.1"
                     /translation="MNLISDEIYRQLVKDSGLNTSLKQLFSHFDTGSDYELLQEQFTQ
                     ARAYFMAAQDSMVQSVRQDLSPLAVYMIKDKASSSGGTFLRWRSMQNARTGGTVWQPI
                     VDDKSVPVEVRKKVVAVEKDRILINMQISVFNHILRQLADCAEKLKEVDNAVAKSDLN
                     S"
     gene            9731..>10158
                     /gene="ssb"
                     /locus_tag="PXD94_001709"
     CDS             9731..>10158
                     /gene="ssb"
                     /locus_tag="PXD94_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6286553.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRYAEICGQYLRKGSKIYIEGKLRTRKWQDQNGQERYTTEIQG
                     ELLQMLDSRNATNSVDGQQSNYQAMSQSPKATHPQDLPMD"
BASE COUNT         3301 a         2004 c         2044 g         2809 t
ORIGIN      
        1 cctcatcaga cgcggggtgg agcagcttgg tagctcgtcg ggctcataac ccgaaggtcg
       61 ctggttcaaa tccggccccc gcaaccacat tccaaaccct cgatagtgaa tcacatcgag
      121 ggttttcgtt ttatttgcca aaaaacagca aaaaataacc gcttgttgac tgcaaaagct
      181 tcctctcgct ttcatttctc gttacacttc tactacattt acgctttttt gattattatg
      241 agcaatattt taactgttac ccaactcaac tattccgtcc gccatttgct cgaaatggaa
      301 atgagccata tttggctaac cggcgaaatc tcaaatttca gccaaccagt ttcggggcat
      361 tggtatctca ctttaaaaga tgacaaagca caagtgcgtt gtgcgatgtt taaaatgaaa
      421 aatgtaagag tgaattttcg cccacaaaac ggtatgcagg tgctggtgcg ggcaagtgtt
      481 acactctatg agccacgagg tgactatcag ctcattattg agagtatgca accggcgggc
      541 gaaggcttgt tacagcagca atttgagcaa cttaaagcaa aactttcagc tcaaggcttg
      601 tttgctcaag agcataaaaa gccaattcct acttttgtta aaaccgttgg tattattacc
      661 tcctcaagcg gagccgcatt acaagatatt ttacatattt tgcaacgacg agatccgagc
      721 ttatcagtgg ttatttaccc ggctctcgtg caaggcaaag aagcaacgca agatattgtg
      781 aatatgattg aactggcaaa ccgccgcaat gagtgtgatg tattaattgt cgggcgaggt
      841 ggcggttcat tagaggattt atggtgtttc aacgaagaga gtgtcgctta tgccatttat
      901 cattcacaaa tcccgattat cagtgcggtg ggacacgaaa ccgatgtgac cattgctgat
      961 tttgttgcag atttgcgcgc accaacgcct tcggctgctg cggaattagt cagcagagat
     1021 caaaatgaat tgaaacgcca attacaacat cagtttgata aagtgaattt agcctttgac
     1081 cgagtttgga accataaatt taaccgtttc caacaactcc ccttacgcct gaatgctcag
     1141 catccaagca aacaaattca atcgcaacag caacgttttg attatatgga ataccgcctc
     1201 gagcaggcaa tttatgcact tgttgcagcc aaaaagcagg tggggaaaga gctaaatgtc
     1261 cgtttaattc accaacatcc gcaaaagcag attgaacgcc aatcccaaat gcttaagcaa
     1321 ttggagctga aactccattg gcaaatggag cagcaactca gcaaaaaaca gcaaaaatgg
     1381 aaaaacatta cgcaacggtt tgaacgcaac ccgctacctt atcaacttta tcagcaacaa
     1441 acgatggtgg caaaaatgtc gcaacgcttg gcttattcta tcgaaaaaat aatgctgaaa
     1501 gagcagcgac attttcaaga gttatgcact aaacttgatg gacttagccc attgaaaatc
     1561 ttgaccagag gctactcaat tacccaaact aaagacggta aaacgcttac ttcaacagaa
     1621 aatattgaac gtggtgaggc tattatcacc caactaaaaa caggaaaaat aataagtcaa
     1681 gtagtggatt ttttgtaggg gaaattttga acgctgccaa tttttaaaat tatttatagt
     1741 aaaatcagta ggttaaatat ttataaacag ggttgttcac ttagttatcc acaggtgatg
     1801 tgcataacta tttttgtaaa gatgaattga gtgctgattt ttaaatcaac cgatttcaaa
     1861 aggcattttt acgcttgcac aagcggtcat atttccctat aatcgtgcaa aaattaagcc
     1921 agtgtggcga aatcggtaga cgcagcggat tcaaaatctt atgcgtaaac tgataggaca
     1981 tacaggcgat tgaataaaaa gcactcttag cctgagtgat gaaattggta gacatgacgg
     2041 attcaaaatc cgttggtgaa taaccgtgtc ggttcgagtc cgacctcagg caccatttga
     2101 atcacacttg tagtttctct ctttcaaata atcaattcaa atacaccgta ttaatggcca
     2161 atactcctta acccttgtta agagagtaaa ttatgaaacc attacaaaac ggtacaaaca
     2221 aaccttttac cattactatt gcttcaacta aaggtggctc tgccaagagt acgaatgctg
     2281 ctaacattgg tgcattctgt gcagaacacg gtctaagaac ccttcttatt gataccgata
     2341 cccaacccac attaagctct tattatgagt tggaatatca agctcccggt ggaacttacg
     2401 aatttctgca ttttaaagat gtagatccta cccacattat ttctagaacg actatcccaa
     2461 atcttgactt gatccagtct aatgacccgt caaataaaat cagcccaatg ctgagagatt
     2521 cacctgacgg tgcattacgt tttagtttac tgctccagaa aattgaaggt tacgatgtca
     2581 tcattattga cactcgtggt accagagata ttactgtcga tatgtctgta ttggcagcag
     2641 atgttttatt ctgtcctatt cttccccaca ttttatctgc taaggagttt attcgtggca
     2701 cgattggaat gtatcaagac ctcgaaacct ttacggcatt tggctttacc ctaccaccgc
     2761 tgaaagcgat gattaactgt gttgatcaca ctaaagatgt caaattggtt tccgactacc
     2821 ttcatactct cttccaaaat gagtttgatg aaaacaaaac attgctggaa ttttcggtac
     2881 ctgaccgcgt ggcatatcgt gaagcggcaa ctttttcaaa accggtctat cgtcagagcc
     2941 aagctgaata tgaaacgatc agtcagcttt gtgcgttact gatgccacag tttgcaaact
     3001 ctcacttctc agcatagagg gagaatgtat gtctagctta aataaatcac ttgtacagcc
     3061 gcattctata gaggctgaat tttcagtaat cggtggatta ctcttggata atgaactctt
     3121 tgatgaagtg agtagcaaaa tcaatgaggg ggatttttat aattttactc accgtctact
     3181 gtttaagcat atttccgaat tgattgaagc cggtaaacca gcagatatat tgacattgga
     3241 tcaatacctt acccagaaaa aagtgatcga ggatttgggc ggatttgctt atcttgcaga
     3301 aatagtcaaa aacactccat ctacagctaa tatccacgct tatacggata tcgttctctt
     3361 atatagtaaa cagcgacagc ttttaaaact ggggcagttt attgttgatc agacacagag
     3421 tgcgaaaacg ccagataagt ttgaagctgt tgttgatgac attgagaaaa aaattactga
     3481 gtttagccta tcagataata caaaatctgc agcagattta agtgatattt ttgccaatat
     3541 gctctctcgt atggaactct cggctaaaaa tggagatcct gtgacaggta ctccaactgg
     3601 tattcaaggt attgacgaag cgacaacagg tggacagcca ggcgatttaa tcgtgattgg
     3661 tgcccgccct tctatgggaa aaaccgcatt ttctcaaact atcgcttacc atacacttaa
     3721 aaaattcgag tcggctcctg tgttctatca cagcatggaa atgccggcag atcagatttt
     3781 acaacgtttt ctagctatgc gtgccagagt gggattgcaa aatattagac aggcagacag
     3841 tttggatgat gaggaatggt caaaactaac ctcagcaatg ggctatatcc tagaacattg
     3901 gaaaaatcgt ttagttattg atgatgaagg tagtctcacg cctcaaaaat tacgcactaa
     3961 agttcgacaa aatgctcgta aatatggcaa accggcagct attttcattg attacattca
     4021 gttaatgcaa acctcgcaac gttatgaaaa tcgcaattta gaaattgcca acatctctcg
     4081 ttcactcaaa ttactggcaa aagagatgga ttgtccggtt tatgccctct ctcagctgaa
     4141 ccggtctgtg gaacaacgtg ccaataaacg cccgatcaac tctgatttac gggaatccgg
     4201 ctcacttgag caagatgccg atgtcatttt attcatctac cgagatgaag tttatgatgc
     4261 ggattctccg tttaaaggca ctgctgaaat cattatcggc aagcaaagaa acggaccact
     4321 tacttccgta atgacgaatt tcagaagtga atttgcccta tttgaaaaca gaacaaacac
     4381 aacgcattag gatagattat gacaaaagat gaaaaaagac tggcagcgat agcaaaagga
     4441 ttaaatgttt cacctatttc aaatacggct ccatcctacc aaacagccac accaacgcat
     4501 tacagtagcg atgttgaata tattacagta acgctggata agttgcgtcc gtatgagcat
     4561 aaccctcgga aaacccgtaa ccctaacttt gagatgatca aagagtctat tcgtcgtcga
     4621 gggttagatc ataagccgaa tatcacccgt cgtccgggag aagatttcta tattatcgct
     4681 gacggtggta atactcgtat tcaggcactc aaggagctct ttaccgagac caaagatcca
     4741 aaattttggt ccatcagttg tcaatataaa ccttggcaag gtgatactgc cgacagtgtc
     4801 gaagctgaac tcaatttact tatcgggcat ttgattgaaa atgatacccg tgcggatttg
     4861 tcatttattg aaaaagcatt ggggattttg caggcgaagg agtattacga gaaaaaatta
     4921 ggtaagtctc tttcttcgag agagttatct tctgagttag aaaatgatgg atatattatc
     4981 tctcatgctc tcattcttaa aatggaacgt tgtgttaatt acttatatcc acatatacca
     5041 gaagttcttt ttagtggtct tggtcataca cagatcgata aactattagc tattcgtaat
     5101 aatgctgaag aggtatgggc agcccatcaa tttgatactg atgttacttt tgaatcgctc
     5161 tggtcagaaa atttatccaa atttaatgag gctacaccat ttcaagcaaa agaattccaa
     5221 agtgaattga ttacggcaat ggttgaagct ttagagggta aagtgagttt tgaaacactt
     5281 tatctggata ttgacttaga tgaacaaaaa ttcaaaaaaa tagcggctaa acaacaagaa
     5341 attgataatt tagttgaaaa aacagtcgat caaatcaata ccagacagga acaacaaact
     5401 gcaaaatctg aacctctcat caaaaatgaa aataaagccg ttactcccca aaaagctcaa
     5461 catattattg atgaggtaat tgatgataac gaaagtgatg atgagcagaa taacgctatt
     5521 actgatttat ctcagcttgg ctcttcattg ctcgataacc aagataatca agaagattat
     5581 ctatcccaaa ttgcccataa ctttggactc actccgggaa tgagtattca agaacagcga
     5641 gagcaacgag ctgaagcgaa tggactgact tttgcttgtg aaggtcgcca accggttgaa
     5701 gatatttggt tgattcaccc cgcacgtcaa tatcgctcag aggcctactc actggcctta
     5761 gatattgccg aaagtatggg cattgaccat ctggttgaac atattgtgaa acaaccagtg
     5821 gattacactt atgcaatgaa accggcagat actgaacttt caccgtctgc tttaacactc
     5881 tactcattcc tctcaatgtt acagactgct gaacataaag atgaaaatag ctgtgtctta
     5941 gattccaaat tcctgtttga gcttgatgat cttaccttag tcaaaatttt ccgtctcatt
     6001 cgggtcagtc gccatattcg ccaacaagga ggagaaaatg attaagccga ttaccgtaaa
     6061 tgaagcattg attggtaacg ccctgctcaa tattaaagag ggaaatatcc atccttgtct
     6121 aaaaatggga ttttctgagg agcaactaaa agcgatcaat aatctcagta ttgatgaaat
     6181 tatggatatt agcaactccg ttgtctcttt tgctaaggtg gagattaatc acgaaacctt
     6241 ttggaaatta ctggctattg cacaagccaa tactcaacag cgacaaatta ttgaccgagc
     6301 attattgctc ggagcctcta ttgaaatgtt gcaccagtat tttggtttat caacctctga
     6361 agtctcggct cgccgtcaat tactcggtat cgaagaaaaa atgggacgaa aagcggcagc
     6421 aagtgatgaa gaatctaccc atatttggga aatttggcaa agatacaaac aacgaatgga
     6481 aatcctagat tcacaagaag gattagagct tctatgtctg attgctgagg aaggcaatat
     6541 gaatctaacg gttgtttgga aactggtttc cgaatggtat tcaaaacata agtaaaaaat
     6601 aaatgccccg aagttggagc tttggggcat tcgatattga gtcttgtatc tctatcttat
     6661 gaattacaag gactttgcag aagtcccgtg cattatagcc ataggagaga gaatagcaag
     6721 agaaaaactc ttttatcctg gtggagagaa acaatgttta gcagcacgaa taagccaaca
     6781 gagcatggat tgctattttt tggcaaccaa cacgaaacag tccctactcg attattacaa
     6841 gatccctgcc ttacaccaag agcaaagttt gcttggcagc tcattaagtg caatgttcaa
     6901 gcctttcaag gaggattatt tccctcttat gagacgctca gcaaaatgtt atccgataga
     6961 ccttatgcgg aggctaaatt atcggaaaaa gtcgtgactc aaacactcta tttactccgt
     7021 cttactcgct ggcttacttt atgtgaaaca gtgcgtgatg agcgaggtta tgtacaaggt
     7081 aatgtttata tcctacatga tgaacctgtg ccaattttag atgcaattca attaaatagc
     7141 gactatctct cttttttaga taaatgcacc aaacataaag atagccttat taatggtgtg
     7201 gcaaatcata ttgttgaaag tctaataaca gacaaaacca aatggcacta tgtctctcat
     7261 ttagactgtt tacaaagtcg ttatcagata taccagcaac agcagctggt gaaagataaa
     7321 cagccatcgc aaacagaaca agaattaagc aaaattcaac aaaacatcct aacttccact
     7381 atggaagtta gggattttga tgaggaagtc aggaaaaaat cctctaaaaa ccagacttcc
     7441 aataagggag ttagggctaa aaatgaagat aagtcattga ttttagactt acttccatta
     7501 gggaagtcag atgtacagta ccgtacttta tatattaata agtactgtac tggggaaccg
     7561 agcgaaatta cttggccaca agaaatccca ttaacttcac ttgaacaaaa acttactgca
     7621 caggcaatga agggattaga cctcggttta tgccaagcta ttttgttgga agtacaacaa
     7681 cgcattattc aaaagcagga tgtgaaaaaa ccgaaaggtt atctctatac gcttatccag
     7741 cgggcgaagc agggtgaatt taaaccctac tattttgaat taggaaaaaa tcttccctca
     7801 cagaatcagg ctaacaataa ttctgctcat tctatacaaa aactgactga gcagcctact
     7861 gagaaaccgg tcttaacttc agcagacaga gagcaaatgg ctagtcgtat gcagaagttt
     7921 agagctgaat tacttttaca ataagattaa aaaacaatca ctgttaaggg gcttaatagt
     7981 gagtgaaaaa taatcagtgt gtaccaggta cacaatggat aaaaaataaa cagcgtaggc
     8041 agtgtctgca aagattaaaa aacagacaat ggaaagcccc tttccaaaga ctaaaaaata
     8101 atcaaataaa caaggagcat atatgaccac tcaattagat cctcaacttg gtccacttcg
     8161 tagtgagatc acttttacgt tacataccca atatgcccat aaattatggg tggggcgacc
     8221 aatgattcgt aatgaggaag gcaaagtaac tcaatcctcc attatcagcg taccaaattg
     8281 ctttgcaatg ctaacccaaa ttcaacgggc tgcatcagaa gatgatcctt atgctgacga
     8341 ttatctgatt caatttgaag aaagtgtgat caattatcgg aaggaaatcc aaaagctcac
     8401 caacgacatt gtcacgctct acgccaaaat gttgccggag ggaatttcta ttgaacgctg
     8461 tgccaatatt tccccgattt cttacccgat ttatgttaat tcacagctag ggtatcaatt
     8521 actctactta cttggtgatt ttgatagcct ttgccgttca gtaatgacgg cagctcatat
     8581 tgcgattatc aatcgtgcag aagctcaaga ttggattgaa gctggtgcaa ggttaatccg
     8641 gaaatgtttt gggatcgttg agcgttataa gagttcagga atcactcgtc gggactatca
     8701 agaaaagaat gctcgctatc aagctgcgat aaaacgaatg ggctatacac tctcagatgc
     8761 tgtacttacc ggtgagcatc gtgctgagtt tgctcccttc atcaagcaaa acactactgt
     8821 tgaagaagag caatccgtag aaacccatat cacggcacag acaaatgaat ctcaaaaata
     8881 aggagtgata tgaacctcat ctctgatgaa atttatcggc aattagtcaa agattctggg
     8941 ctgaatactt ctctgaaaca gctatttagc cattttgaca ctggctcaga ctatgaactt
     9001 ttgcaagaac agttcactca agctcgggct tattttatgg ctgcacagga tagtatggtg
     9061 cagtctgtgc ggcaggatct ttcacctttg gctgtttata tgattaagga taaggcctca
     9121 tcctcaggcg gtacattttt acgctggcgt agtatgcaaa atgccagaac cggtggcaca
     9181 gtttggcagc cgattgtaga tgataagtct gtcccagtgg aggtgaggaa aaaagttgtc
     9241 gcagtagaaa aagacaggat tttgattaat atgcagattt cggtgtttaa tcatattttg
     9301 cgacagttgg cagattgtgc tgaaaagttg aaagaggtag ataatgccgt tgcgaaatca
     9361 gacttaaatt cgtagtaaat ttatatataa caactcctat attggttgtg tgtaaacgcc
     9421 aaccacggtg ggctgacgtg gtaaaacaaa cagtccgtta attaggacat attcaagttt
     9481 gtaatacctg aaaatccggt ttgtaatccc tgaaaaagtg cagtttgtaa atcctgcatt
     9541 aaaaggcctt ttaaaggtaa gggtacaagc cccttacctt cacgaagaaa aagcctttac
     9601 aacggaggaa taagtgattg cacacgtaaa aatgaaaaat tcactctaac caaattttaa
     9661 ttaaatactt ttttcttagg ctgaatcaag tttaatagaa ctaacttaat ttattgaagg
     9721 agtaaatacg atggcaggaa tcaataaagt gattattgtc ggtaatcttg gcaacgatcc
     9781 tgaaatgcgg actatgccta atggtgaagc agttgctaat attagtgtgg caacttctga
     9841 aagttggacg gataaaaata ccggagaacg tcgagaagtc actgaatggc atcgtatcgt
     9901 tttttatcgc cgttatgctg aaatttgtgg tcagtaccta cgtaaaggct ctaaaattta
     9961 tatcgaagga aaattacgga cacgtaagtg gcaagaccaa aacgggcaag agcgttatac
    10021 aaccgaaatc caaggtgaac tattacaaat gttagatagc cgtaatgcta caaatagcgt
    10081 agatggacaa caatctaact atcaagctat gagccaatct ccaaaagcaa cacatccaca
    10141 agatttacca atggataa
//
LOCUS       DANLXQ010000090         9887 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.090, whole genome shotgun sequence.
ACCESSION   DANLXQ010000090 DANLXQ010000000
VERSION     DANLXQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.090"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(67..561)
                     /locus_tag="PXD94_001710"
     CDS             complement(67..561)
                     /locus_tag="PXD94_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6286554.1"
                     /translation="MLSGLKIESFSKEFKRDCFDSESELLNQYLKTQVSQDIKRRITS
                     CFLAVNSEQTVVGYYTLASTSLSLSDLPITLQRKLPRYPTVPAVLLGRLAVDKRVKGL
                     GLGQALLGNALKRVLRADIAAYALIVEAKDESAVNFYRKFGFIDFENEARKLFFPLDR
                     VRLS"
     gene            complement(561..833)
                     /locus_tag="PXD94_001711"
     CDS             complement(561..833)
                     /locus_tag="PXD94_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL6286555.1"
                     /translation="MATATARFEARMSQEVHMLLKRAASLEGRSLSDFVVGAALSAAR
                     KTVEQNDIIRLSVNDQELFAKALIDPPEPNLAMQKALALSSQILGE"
     gene            complement(992..1420)
                     /locus_tag="PXD94_001712"
     CDS             complement(992..1420)
                     /locus_tag="PXD94_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286556.1"
                     /translation="MRKLLVITALTLCTTPVFAADKNVIFSCTSTEGKPLTVKRVGND
                     YEYSYDKTTFKNPIKKAVTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKE
                     FIEPGATISQSKEQPSIGSVDCDTRKKSHYKFDVHLMNTL"
     gene            complement(1441..3447)
                     /locus_tag="PXD94_001713"
     CDS             complement(1441..3447)
                     /locus_tag="PXD94_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6286557.1"
                     /translation="MKSQFKFKKTHLLVITVLFSSTSSISFADSITDKLEFSKSDSII
                     SDRVFYQIGGGTGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMILQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSTQPDAKQAQKDLEDKKGKDGRVWVGGQRKGGQNQ
                     DPINVVGDVAKGGFNLLNKRNVLDSSPVSNCEGNLCQTWDTPQAAADFLTKIIGEQSI
                     STCKEDCGTKTSSKAGVGLSPEIEEEHIRTIVKLENVLNMDVPKSELLAELSSSSVPV
                     TRGLIESLRDDPDVELLSQRLSAEIATSKVMEKMLLARRAILAGMREPNVAQNADAQV
                     ELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLDKR
                     VQGLENGNVALTDLETGSNINLSRRNIALSIPNIPNVAALIPTLPNRGGSGVGGNFPS
                     LAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTVVKVTKDTVI
                     TKEDAERDLARRTAIFANNVRQELGDSHWNALPANAQAVLVSYAYNYGSLAKTKSVLD
                     AARRSAQTGDMTALATAVRNRQVDNNGINARRRNQEADYILGKN"
     gene            complement(3462..4403)
                     /locus_tag="PXD94_001714"
     CDS             complement(3462..4403)
                     /locus_tag="PXD94_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286558.1"
                     /translation="MKRAFFSVLLSVSLAVPTTLYPQQVKASLNTASIAASSASTSCL
                     DYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNQDGQTPWTELQSLNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGSTGYYCKSQTTPFIPYFLSGLDFFA
                     WRENMPEMFYPEALIPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     TRTAQPHIYFPAAKKGGVGEWPPGPVEEGQARTHKWQMLYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            complement(4400..4843)
                     /locus_tag="PXD94_001715"
     CDS             complement(4400..4843)
                     /locus_tag="PXD94_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL6286559.1"
                     /translation="MKASRFLGYFSIVTVVFSVVAQAQPNIIVYTTKMYPIQNSQLAS
                     QVYYLDQVENFEEEISRNLSKSPEIAEREIRAFFNSADWKAQEEQLKDAYQGVISGWQ
                     NGIKKVPAVFFNNLQSSPSVIYGMTDIQKALDEWHKWVQQHGESK"
     gene            5218..5571
                     /locus_tag="PXD94_001716"
     CDS             5218..5571
                     /locus_tag="PXD94_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286560.1"
                     /translation="MSQQTAGWDVIPFESSKTAKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFRALFVAIIAF
                     LCGMATVFGTQLSLI"
     gene            5643..5888
                     /locus_tag="PXD94_001717"
     CDS             5643..5888
                     /locus_tag="PXD94_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6286561.1"
                     /translation="MSVHIKLSCVGDILPIKLNEWTPEHRALHHEISHKALEAWQNRD
                     KGTQSVRFMQQMPARQGLHFRFLSVSEQDGKLIVSRS"
     gene            5897..6139
                     /locus_tag="PXD94_001718"
     CDS             5897..6139
                     /locus_tag="PXD94_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286562.1"
                     /translation="MFGKLFYKMNPKVLAKIGEDFRKAGYLMGAGFVGMVISNDQIST
                     SEGFVLFLWGLYIWIIGHIALHHSEKILNSKQGEPK"
     gene            6403..7245
                     /locus_tag="PXD94_001719"
     CDS             6403..7245
                     /locus_tag="PXD94_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286563.1"
                     /translation="MSDSVLNTYFKHALVKAGFPDTLDMEYSLSHCQGDGVAFYGRLG
                     TDDLIQLFNHLNPQKRKQKMFANLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMEL
                     YAKTADQLVFFNDSDARRNWYFPTSKVDKYKALWDGFIEDLEQYIKDISREMESVGYQ
                     IIEASPYQTETVFKFNTEKYLVELKRKPSEFYSELVDWAFGDMCDFEAAIEQICQGQL
                     CYADFEATVTDKKSEIQLGLARLSGISYSPNDRSFSGYRQELIGEAIASARHNAARFA
                     YLHA"
     gene            7360..7773
                     /locus_tag="PXD94_001720"
     CDS             7360..7773
                     /locus_tag="PXD94_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6286564.1"
                     /translation="MKNEHNLEQLKYIWDNRNLENKVYQYLFHICIEPMKKGDWNRYT
                     IWLNFHGNLINVSDVVYRVVESQKLKDDIKIWYDKQYYCRPTKVETTGFISIFLHLFY
                     EGIARILLEQGMITETEAAEKSYNFRAANCFQRLL"
     gene            7787..7942
                     /locus_tag="PXD94_001721"
     CDS             7787..7942
                     /locus_tag="PXD94_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286565.1"
                     /translation="MEPRTVKVIYDDGDYFFTLINADEQTIRNYYAIGSSIGFDNTRK
                     IVAVEIL"
     gene            8022..9005
                     /locus_tag="PXD94_001722"
     CDS             8022..9005
                     /locus_tag="PXD94_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL6286566.1"
                     /translation="MNPQSNTQPKFDLYQEVTDKIIAALEQGTAPWLKPWDSVIPYGG
                     MPCNAISGRLYSGINVLLLWLSAAEHGYIQRKWITAKAAIEAGGHVRKGEKATLAVSY
                     RPYDREKTDDDGNLVLDDDGNPVIEHLASIKKHCLFNIEQCEKLPQDWYETVETPEQL
                     TDENQYAIFEEIRLMLKGMDLQVMVKPSNQAYYHPRNDHIVMPEMKQFHSPQKFHSVL
                     LHEMTHATGHQSRLARDGIVSGKARFGNKVYAFEELVAEMGCAFLCSHLGFADVPQNA
                     SYIESWIKVLREDKRAIFRASGKAREACQYMLDALNIAQLADKYEVFEEAA"
BASE COUNT         2879 a         2001 c         1922 g         3085 t
ORIGIN      
        1 ttctttaaaa gctcgaattt tcctgcctaa ccaataacca aaattatttt tcataatcac
       61 tctccattaa gataatctca ccctatctag tggaaaaaat aatttcctag cctcattttc
      121 aaagtcaata aatccaaatt tacgataaaa attgaccgca ctttcatctt ttgcctcaac
      181 aattaatgcg taagctgcta tatctgcacg aagtaccctt ttaagtgcat tacctaatag
      241 agcttgccct aaccctaatc cctttacacg cttatctact gctaatcgac caagtaggac
      301 ggcaggtacc gtaggatatc ttggtaattt cctctgtaat gtaattggta aatcagataa
      361 tgaaagactt gttgaagcca gtgtgtaata tcctactact gtctgttcag aattaacagc
      421 taaaaaacaa gaagtaatac gtcttttaat atcttgtgaa acctgagttt tgagatattg
      481 atttaataat tcagattcac tatcaaaaca atctcgttta aattccttgc taaatgactc
      541 tattttcaat cccgaaagca ttattccccc agaatctgtg agcttaatgc caaggctttt
      601 tgcattgcca aattaggttc aggaggatca atcaatgctt tagcaaaaag ttcttggtca
      661 ttcacagata atcgaataat atcattttgc tcaactgttt ttcttgctgc agatagtgca
      721 gctccaacaa caaaatcact aagactacgc ccttcaagag aagcggctcg ttttaacagc
      781 atatgaactt cttggctcat tctagcttca aatcgtgcgg ttgcagttgc catatatttt
      841 ctccttgaat tatttagttt taattgtacg tatttatacc gtacaaatca agcatgacaa
      901 taaaaaaatc ccttagattt tcaaacctaa gggatttacc taccgatctt tgtgtttttt
      961 aacgtgggct ggccctagtg ccatacaggc attataaagt attcatcaaa tgaacatcaa
     1021 acttataatg gcttttttta cgagtatcac aatcaacaga gccgatactt ggttgttctt
     1081 tactttggct aatcgttgct cccggctcaa taaattcttt tgcattgcca tttggctgaa
     1141 caaagccgac caaatacttc aatccatcat tttccagctc taatgcatac gttgtaaaac
     1201 ctgaacctct ggcgataatt gagccgtcat tagtgactgc ctttttaatc ggatttttga
     1261 aggttgtttt gtcatacgaa tattcgtaat catttcctac ccgtttaaca gtaagcggtt
     1321 ttccctccgt gctggtacag gaaaatatta cgtttttatc tgctgcaaat actggggtcg
     1381 tgcagagtgt aagagcagta attactaata atttacgcat aaatgtaact ccttataagg
     1441 ttaattttta ccaagaatat aatcagcttc ctgattccgg cggcgtgcat tgataccgtt
     1501 attatcaacc tggcgatttc tgacagcagt tgccaaagca gtcatatcac cagtttgagc
     1561 tgatcgtcta gctgcgtcaa gcacactttt tgttttcgct agtgagccgt aattatatgc
     1621 ataagacacc aaaacagcct gagcgttcgc cggtaatgca ttccaatgtg aatcacctaa
     1681 ctcttgtctc acattgttcg caaagatagc agttctgcga gctaaatcac gttcagcatc
     1741 ttctttggta atcacagtat ccttggtcac tttaactacg gtaccgtctg cccgtgtaat
     1801 cgtatcggaa ccatatccta cacgatgggc attcacatcc cattgagcct tggagataaa
     1861 gccttcaaaa ttacgcagta accctgttgc ctgatccatc gttgaaccgc tgatttgtgc
     1921 aagtgatggg aaatttccgc ctacacctga accacctcta tttggtagag tcgggatgag
     1981 agctgctaca ttcggaatat ttgggatact caatgcaata tttctacgag agaggttaat
     2041 attagagcct gtctctaaat cagttaatgc aacattaccg ttttcaagtc cttgaacacg
     2101 cttatccaga ctatcttggg ataaactatg agcaccagca agagcttccc gttcggattt
     2161 actttgcaga atcaccgaag ccgtattgcc ggtgatcatt ttttgcatat ccatttccag
     2221 ttttacttgg gaaatttcac ggtcaataat attgagtact ctttccagtt ctacttgagc
     2281 atccgcattt tgagcaacat tcggctctcg cataccagct aggattgctc ggcgagccag
     2341 tagcattttt tccattactt tactggtcgc aatttcagcg gataaacgtt ggctgagaag
     2401 ttcaacatct ggatcatcac ggaggctttc aatcaagcca cgagtgacag gtacactact
     2461 tgagcttaat tcagctaata gctcggattt cggcacatcc atattcagta cattttctaa
     2521 tttaacaatt gtacgaatat gttcttcttc aatttccgga cttaaaccaa caccggcttt
     2581 cgaactggtt ttagtaccac aatcctcctt acaggttgaa attgattgct caccaataat
     2641 cttagttaag aagtcggcag cagcttgagg tgtatcccaa gtctgacaaa gattaccttc
     2701 acaatttgaa accgggctgc tatctaagac attccgctta ttgagtaaat taaatccgcc
     2761 tttcgctaca tcaccaacga cattaattgg atcttggttt tgcccacctt tacgttgccc
     2821 accaacccaa actctcccat ctttcccttt tttatcttct aggtcttttt gggcttgctt
     2881 cgcatcaggt tgtgtcgagg taatttgctt atagctctct aaatctgctg cttgccccca
     2941 ttttcctcct agtgtgtgat ctgccagaac tttagacatt tcctcacagg aagtttttag
     3001 acggttgaaa tccagcttac cttggtacat accatttgtc agcaaatcgt agagttgagg
     3061 gttggcccgt tgcaatatca ttgcaggcat actggcaacc gcacctttag ctgaatcaat
     3121 gacattcccc attaaatctt taaagccatc tttaaggccg tttaaatcat ttttaatagt
     3181 tgttttaatg tcaaaattgc cacaggttaa attcgctttc caaccgatgc ctaaatcaac
     3241 gactttggta ttaccacgac taggtggagc catataacct gttcctccgc cgatttggta
     3301 aaagacacga tctgaaataa tcgaatccga cttactaaat tccagtttat cagtgattga
     3361 gtcagcaaaa gagatagagg aagtactgga aaaaagtact gttattacta atagatgtgt
     3421 tttcttgaat ttaaattgtg atttcattgc aagacctcgt tttagtgtga ccaatcaata
     3481 ctgtaaagga atgtttgccc tcggcgttta caacaactat aaggtcgcca tagtgcccaa
     3541 gcataatcac cttgatttga aatctgagag gaataggtgt ctgtttgccc cccatttgga
     3601 aaaacagagc aggtatttga catttgagga taaagcattt gccatttatg tgttctggct
     3661 tgtccttctt cgacaggtcc tggtggccat tctccaacac cacctttttt cgctgccggg
     3721 aaataaatat gtggttgagc agttcgggtc acgatgtctg caactctctg cacaatggtt
     3781 gcagaggctt tataatcgtg agcttgtgtt acccaaccgg aacgtgggta aatatttccc
     3841 cagctatcgc ctacacggcc aacttcccgc attcccggaa tcagagcttc cggataaaac
     3901 atttccggca tattttcacg ccaagcaaag aaatctaatc cacttagaaa atagggtata
     3961 aatggtgtgg tttgactttt acaatagtag cctgtacttc ctaataactg accaattgct
     4021 ccttggggat gcccgaaagc atctgcattt ttgaaggtta gttgagagta ttgacgttgc
     4081 ggagaacgat attctccgcc ttttacgcca ggattgagag attgtaattc tgtccacgga
     4141 gtttgcccat cctggttata agatgaaacg accatttccg gtaaataatg cttaattttg
     4201 gttgatgtac gaaccttgca tccaaagtag gtgcaaaata accagaagca cgttccgacg
     4261 actttatagt ctaaacagct tgttgaggca cttgatgcag caatcgacgc cgtattcaat
     4321 gatgctttta cctgttgagg atataaggtt gttggtactg ctaaacttac agagagaagt
     4381 accgaaaaaa acgctctttt cattttgatt caccgtgttg ttgaacccat ttatgccatt
     4441 catctagtgc tttttgtata tcagtcatac cataaatcac gctcggagag gattgcagat
     4501 tattaaaaaa caccgcaggc acttttttaa ttccattttg ccatccactg atcacccctt
     4561 gataagcatc ttttaattgc tcttcttggg ctttccaatc tgctgaatta aagaatgctc
     4621 gtatttctcg ttcggcaatc tcgggggatt tactgagatt tcgactgatt tcctcttcaa
     4681 aattctctac ttgatcaaga taataaactt gggaggcaag ttgtgaattt tgaatggggt
     4741 acatctttgt tgtatagaca atgatattcg gctgtgcttg tgccactaca gaaaacacta
     4801 ctgttactat gctgaaatat cctagaaatc tacttgcttt catcactctc tttccgaata
     4861 aataagcaga aaattagcga gatttttaca tagaggatag gaaaaactct tttcaaaaaa
     4921 gagatatttt tttgagcaaa aaatggttga ttttataaat ggatttcgta tgatatggaa
     4981 tattaagatg ctgtggaaac agcccttaaa agtcgcataa ctcttgagaa gcggcggcta
     5041 tccatcgagg tagttgagtt atttacccaa aatgtctccc agcattctga aagggaacgc
     5101 tgcaaatttg caggaatgcc acctaaacac ctaccttccg agggatattt caattccctt
     5161 ggaattgcga tctcctgagg aattttattt atttaacttt ttataggaga actcgctatg
     5221 agtcaacaaa ccgcaggatg ggatgtgatc ccatttgaat cgtcaaaaac cgcaaaacct
     5281 tctctttttt ggaaacttgg caataagctc ggtaatattt tcgtttcggc gaaacataaa
     5341 gctaaaagca aatatgtcga ggtaaccaaa ggcatcaatc aacgaattgc tgaagaagat
     5401 gaaatgcgag ccttagaatt gcaaatgtgc ttagatgagc aagcagctga atatgagcag
     5461 gaaatttctc gcattaagcg taagatgttt ttcagggctt tattcgttgc gattattgcc
     5521 tttttatgtg gtatggctac cgtttttggt actcagctct ctttaatcta atttaactca
     5581 cccatcgggg aaatgctatt tcccttgtgg taatacattt ccccttaatt ttggagaaat
     5641 gtatgtcagt tcatatcaaa ctctcctgtg ttggagatat tttgccaatc aaactcaatg
     5701 aatggacacc ggaacatcga gccttacatc acgaaattag ccataaagct ctcgaagcct
     5761 ggcaaaatcg agataaaggc actcaaagcg tgcgatttat gcaacaaatg ccagcccgac
     5821 aaggactgca tttccgcttt ctgagtgtat cggagcaaga cggcaagctc attgtcagtc
     5881 gttcatagtt ggttcaatgt ttggtaagct tttttacaaa atgaatccca aagtactagc
     5941 taaaatcgga gaagatttta gaaaagctgg ttatcttatg ggagctggtt ttgtcggaat
     6001 ggtaatttcc aatgatcaga tttccacatc ggaaggattt gttctttttt tgtggggatt
     6061 gtatatttgg attatcgggc atatcgcact acatcattct gagaaaatac ttaattcaaa
     6121 gcaaggagaa ccgaaatgag tccgtacttt ttaggtgctg ctattggaac agcgttattg
     6181 gcaacaattt ttattgttct agtaacttgg gcaaatcgag atattttgtt tacaaaatca
     6241 gataagccac agtctaaata gcaaaactcc attctaaccc ataggggaac aagttccccg
     6301 aggagcttgt tctccgtttt accttccttt taccctatcg ggaaatactt tcccgaattg
     6361 ggcttaagtg tttcccatta ttttttattt tggagaaaca ttatgtcaga ttcagtatta
     6421 aacacttatt ttaaacacgc tcttgtcaaa gctggttttc cggatacctt agatatggag
     6481 tattcactca gccattgtca aggagatgga gtcgcatttt atggtcggct tggtacagat
     6541 gatctaatcc agcttttcaa tcatctcaat ccacagaaac gtaaacagaa aatgtttgct
     6601 aatttgctaa cgcacattga atcttgggaa acccacgatg atattggttt tgaaattata
     6661 cgaaatcgct ttgggtatcg ttactcgcat tggaatacga tggagcttta tgctaaaact
     6721 gcagatcagt tagtgttttt caatgatagt gatgctcgaa gaaactggta ttttcctact
     6781 tcaaaagtgg ataaatacaa agcattgtgg gacggattta tcgaagattt ggagcagtac
     6841 atcaaagata tttcccgtga aatggaatcg gtcggttatc aaattattga agcctcgccg
     6901 tatcaaacgg aaaccgtttt caaatttaat acggaaaaat atctagtaga actgaaacgt
     6961 aaaccttctg agttttattc agagcttgtt gattgggcat ttggtgatat gtgtgatttt
     7021 gaagcagcta tagagcagat ttgtcaaggg caactctgtt atgctgattt tgaagcaact
     7081 gttaccgata agaaaagtga aattcagtta ggtcttgctc gcttgagtgg aataagttac
     7141 tcgccgaatg accgttcgtt tagtggttat cgacaagagc ttattggtga ggcgattgcc
     7201 tctgctcgcc ataatgcagc acgttttgcc tacttgcacg cctaatttgc agatttttac
     7261 tttaatttga ccgcttgtag tggttaattt tcaccccgtg gggaaatatt ttccctattg
     7321 ggcaaaatgt ttctccactt tttaatttgg agaaatgtta tgaaaaatga acacaattta
     7381 gagcaactta aatatatttg ggataaccgc aaccttgaaa ataaagtcta tcaatatctg
     7441 tttcacatct gcattgagcc gatgaaaaaa ggagattgga atcgttatac gatttggctt
     7501 aattttcacg gtaacttaat caacgtgtca gacgttgtat atcgagttgt ggaatctcaa
     7561 aagctcaaag atgacatcaa aatttggtat gacaagcaat actattgccg tccaaccaaa
     7621 gttgaaacaa caggttttat ttccattttt ctgcacctgt tttatgaggg catagctcgt
     7681 attttgctgg aacagggaat gattactgaa actgaagcag ccgagaaaag ttacaacttc
     7741 agagctgcaa attgttttca acgtttgctt taatggaggt gagataatgg aacctcgcac
     7801 tgttaaagtt atttacgatg atggcgatta tttctttacc ctaatcaatg ccgatgaaca
     7861 gactattcgt aattactacg ctataggcag ttcaatcggt tttgataaca caaggaaaat
     7921 tgtcgctgtt gaaattttat aaactaccca tcggggaaac gacttcccca tagggcgata
     7981 gtgtttcccc atttttctac ccgaactttg gagaaacact tatgaaccct caatcaaaca
     8041 cacagcctaa atttgattta tatcaagaag tgaccgataa aatcatcgcc gctttagagc
     8101 aaggaacggc tccttggctt aaaccttggg atagcgttat tccttatggt ggtatgccgt
     8161 gtaatgccat atccggaaga ttgtattccg gaatcaatgt tttgttactt tggttgtctg
     8221 ccgcagaaca cggctatatc caacgcaagt ggatcactgc aaaagcagct attgaagccg
     8281 gtggacacgt tcggaaaggt gaaaaagcaa cccttgcggt cagctatcgc ccgtatgatc
     8341 gagaaaaaac cgatgatgat ggcaaccttg tcctcgatga cgatggtaac ccggtaatcg
     8401 aacatctcgc ttcgattaaa aagcactgcc tattcaatat tgaacagtgc gaaaaactgc
     8461 cacaggactg gtatgaaacg gttgaaaccc cagaacagct gactgacgaa aatcagtatg
     8521 ctatttttga ggagattcgt ttgatgttga aaggtatgga tttgcaagtg atggtaaaac
     8581 caagcaatca agcttactac cacccacgca atgaccatat cgtgatgccg gaaatgaagc
     8641 agtttcattc accacaaaaa ttccatagcg tgctgctaca tgaaatgact cacgcaacag
     8701 ggcatcaatc tcgccttgct cgtgatggta ttgtttccgg aaaagctcgc tttggcaata
     8761 aagtttatgc ttttgaagaa ttggtcgctg aaatgggctg tgcattcttg tgttctcacc
     8821 taggctttgc tgacgtgcca caaaatgcca gctacattga aagttggatc aaggtgttaa
     8881 gagaggataa acgggcaatt ttcagggctt cagggaaagc tcgggaagcc tgtcaatata
     8941 tgctggatgc cttaaacatc gcacagctgg ctgataaata cgaagtattt gaagaagccg
     9001 cttaataaat ttgccgaaag gcatttcccg tggggagata ctctcccttc ggggcattgt
     9061 gtctcccttt ttattcactt aacgagagga ctcaatgctc aatctaaact tatttaaacg
     9121 acccattgaa atttcgacac ttgaatcttg gtcaaaattc tgtgatgatg ttggtaaagt
     9181 agcttttgtt gcagtacctg ctatgctgta tagtaattac gagttttgga ttaagataag
     9241 cagtataatt ggtctattta tagctaccta tttctgttta gttatcggta gaaatatacg
     9301 ccaatttatt gagaaggaga aatcaaaatg ggattaatga tcggcatttg gattattgct
     9361 tttattgcat ttgggttggc catatggaca agccatattc taaaaaagca agatgaacgc
     9421 cttaaacagc taaaaaatag ctaactttca aaatacggca cattttgtgc cgtatttctt
     9481 ttgtactggt ggcaaatata agattactat catcagtata ttgtaatatc tcaactcaac
     9541 agcattagtc aaaaatcctc caacatattc agagagtttt tcctactgag ttctcttatt
     9601 ttttggttca atactctccc tttataaatt aaatttggtt cagataaaag ccgctcaatg
     9661 gcaaaaggat aaaggagccc ttacgggcaa gggtaatccc ctaaggggga ataagggtaa
     9721 cctttccaag gggaaaggtc ttacccaaaa gggcaaaggg ctaaacggct ttctttcaac
     9781 gcaaacatca tctttttcgg tctatttcaa tgcactcaat tctcaaaaaa attcttggca
     9841 aaagtaaatc ctcgtctcaa agttcggtta aatctgacaa tgttttc
//
LOCUS       DANLXQ010000091         9870 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.091, whole genome shotgun sequence.
ACCESSION   DANLXQ010000091 DANLXQ010000000
VERSION     DANLXQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.091"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            176..1882
                     /locus_tag="PXD94_001723"
     CDS             176..1882
                     /locus_tag="PXD94_001723"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL6286567.1"
                     /translation="MNTSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLAAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATDSPVAIIVTSGTAVANLYPAVIEASLSHQK
                     LIILSADRPTELIGCGVNQAIHQQGIFANYPVASVNLPKANEAYHASWLVSTIEQACT
                     TQAQQGGVLHINVPFEEPLYEADINEILNTPWLRPIQGWLNNAQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKTWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIVIQFGTQIVSKRVNEFLKAFQGEYWVVEQSHDYHNPNAHHQTRF
                     IAKAHHFLRVHPPLRQKPWLLEPLALSQFCSTFIEQQVGGSLSEASLAHNIQDVLAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGALFDMLPVDSKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            1931..2770
                     /gene="menH"
                     /locus_tag="PXD94_001724"
     CDS             1931..2770
                     /gene="menH"
                     /locus_tag="PXD94_001724"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL6286568.1"
                     /translation="MLSYQWHAQQGTPVVFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFANLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMEN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(2819..3496)
                     /locus_tag="PXD94_001725"
                     /pseudo
     CDS             complement(2819..3496)
                     /locus_tag="PXD94_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3582..4244
                     /locus_tag="PXD94_001726"
     CDS             3582..4244
                     /locus_tag="PXD94_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDL6286569.1"
                     /translation="MRKTHSFQQVRKIKPTWMSVSGHIPFRNGVSIPYESTLERDFLM
                     YFTYLPSVDEVVSQPTTLPFVKNGITYTYTPDFFLSFTDGRKPMLIEVKPKAKWQKHW
                     KEWKEKWKAAIRFCQENGYVFHVYDEDRIRHLALFNLNYVQRYKRIQHEQEDIDAILA
                     QVKLMGNTTIDYLLSRFFAGSLYRMKGLQIIYHLLATKQLHCNWFLPLNEFTEVWGNN
                     DE"
     gene            4234..6162
                     /locus_tag="PXD94_001727"
     CDS             4234..6162
                     /locus_tag="PXD94_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL6286570.1"
                     /translation="MMSNSGFTEKRYHHRLDRGRIILQKGNIYLNREDGEQYELVDYM
                     DEPSQLLVRNLNTRTTKVVSIHQLENFKMNERTDLSVDVTAISNEYWEKAQQKYEAIK
                     PLLGMDQHRPYAVKARAEDVGVNPRTLYRWLQAYNSIGSIAGLVDQKRGWQQGNSRLT
                     PEQDKLIVQVINEFYLHKQRPTTEQTIREIRRRCKIEKVESPSKETIRMRILHISEEE
                     RLRKRGQREKARNKFKPKPNSFPDADYPLSVVQIDHTPVDLIIVDSKYRKPIGRPFLT
                     VAIDIYSRMIVGYYLSLDAPSVTSVAMCIARGILPKERLLLDLGLQGSEWNAFGYPVK
                     VHVDNGPDFQALDLSKSCSAHGIHLEFRPMGRPEYGGHIERVIGTFMKEVHSLAGTTF
                     SNIKERDSYDSEKEAIMTLDEFEKWLVHYIVNVYHKRVHSALGISPEQKWKIGIFGDE
                     NEVGCGYPQLPVDEQTLLLDFLPSITRTIQHNGVTIDGLRYYDVALNMYISDSDESGK
                     SKEFLFRRDPRNISKIWFYDPKLKRYFPIPFANQAMPEMSIWEYREVRSRIANKGDKY
                     INEQQVLDGLTEMREMVAESAQRTKKARRQAERQKMHKASKPIIETKVETKAVVPVVV
                     TSNLLALDDESLSFGEVD"
     gene            6162..7049
                     /locus_tag="PXD94_001728"
     CDS             6162..7049
                     /locus_tag="PXD94_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDL6286571.1"
                     /translation="MQYEHVHEKFRHLVTADNQERIAFLDEPRWLGYGVAKDIMDNLV
                     SLMNKPKRPRMLNLLIVGDSNNGKTTLIRRFFDLYGQAYIDSDSNAIYPILLAEAPPS
                     ANEKELYISLLERFYVPYKPTDTIAKLRYQTIHLFREFRVKMLVIDEFHSLLVGTPRL
                     QRQVMNAIKMLCNELQIPIVGVGTRDAIRVLHTDRQHASRFDVAELPIWKLDKEFQKL
                     LFQFQGILPLRESSNLQSPELATKIHTISGGNLGNVHRLLTACAVQAITSGTEKITLD
                     IIEKNSWVQPTNGYRKIIG"
     gene            7007..8113
                     /locus_tag="PXD94_001729"
     CDS             7007..8113
                     /locus_tag="PXD94_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDL6286572.1"
                     /translation="MGTTNKRLSQNNRIKKGVIHTPIYSNESISSWLIRASFDCGTDP
                     LTFTGFYWEKHRLWTLDLDRGLENIEPTIYDDVQALSGKIQLSEYSLFRRLYPINADE
                     TLAKGQVRWVIPRSARNRTHHIGQQYCPLCLDESPHLRNDWRLAWNFGCLKHNMMLAN
                     RCFCCGTLYQPHLLSAEKRHLNHCHQCGGKLEQGTMEMSLAEKLTLQGINQVLEKDSG
                     ICFGYKVSGKVWFDMLRYCINLLRRASDRPNHALAKFVVNLQIDVENICSPKTGLSFE
                     QLPLGERTNLLVNAYHILGMEADSIRSAIYLCGGTQKTFMFEKYPDFWQEFMALAPAG
                     PNRTRKRTKPQNESITAINRRWERLKRKLHLNNE"
     gene            8110..9534
                     /locus_tag="PXD94_001730"
     CDS             8110..9534
                     /locus_tag="PXD94_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286573.1"
                     /translation="MKINVIKPQEEPCSACGKISRIWRRYNGEGYCANCYAKWFPKKE
                     CSCCHQPKRIYLSSTVCWDCEKQTDCVRCGKAAGTFKIGKITRYGAVCNVCSRYFREE
                     RECFECGQLSRTMLRASESGHSELLCLKCFQKYRFATCKNCRRYRKVHDRDNQLCRKC
                     SEQRTSQCPKCKQDMPAGYGNICADCSRRTLLFNLIRMNVHLFRHKKVKRAYKKFIFW
                     YLAKCGVSVVLKKSDDHVLFFMRCDENWGNIPDYRELVTFFKPNGLRTHLTVLRWLLD
                     TNQVKVDESLKEEMAGLERIQSLFNKLSNSVPCIANYYTQLQHKYEAGKTSLKSVRLA
                     LQPAIDFISSESVSLLPTQVQLNSYLVAKTGQIAAITGFINHLNREYHCELEIDRSLI
                     QKMKEKQLKQQAKQRLAILYKQEKLSIKEQNELLSVVLLNLHNIWIKSPKKLSISEID
                     GIAYYVDNEKKYFIPKDIHIRLME"
     gene            9649..9855
                     /locus_tag="PXD94_001731"
     CDS             9649..9855
                     /locus_tag="PXD94_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA delta(2)-isopentenylpyrophosphate
                     transferase"
                     /protein_id="HDL6286574.1"
                     /translation="MTLKAQFREWLKKEQEHDEMIGFAHIPCILSKVDSKLAIVSGNA
                     YEKKYLRYWIKCFRFDRNISKYIK"
BASE COUNT         3054 a         1989 c         2127 g         2700 t
ORIGIN      
        1 taaatgctct taaaaaagag agagtttact aagtaaagtg ttgctcaata gatagaaatt
       61 ttcacgaagt tttacaaaaa cattccaatt ttctcctaaa aaacttccca caataatttc
      121 actttttgct acaatcctaa ccatttatcg aatttatcaa tcagtagaga agaatatgaa
      181 taccagttct tttaatcgca cttgggcaaa agtgatttta agtgcattaa tgcgttatgg
      241 cgtaaaacat ttttgtattg ctccgggttc tcgctcaaca ccgctcacat tagcggcagt
      301 gcaacttcaa caaacgcaat atgcagagtg ccacactcat tttgatgagc gtggtttagg
      361 tttttatgcg ttagggattg ctaaagcaac cgacagcccg gttgctatta ttgtcacttc
      421 cggcacagca gtagcgaacc tttatcctgc ggtgatagag gcaagcctga gccaccaaaa
      481 actgattatt ctttctgccg accgcccaac cgaattaatc ggctgtgggg taaaccaagc
      541 cattcatcag caaggtattt ttgccaatta cccggttgcc agtgtcaatc taccgaaagc
      601 gaacgaggct taccacgcaa gctggctggt ttcgaccatt gagcaagcct gcactaccca
      661 agcacaacaa ggcggtgtgt tgcatatcaa tgtgcctttt gaagagccgc tttacgaagc
      721 agacattaat gaaattctaa atacaccttg gttacgccca atccaaggct ggttgaataa
      781 cgctcaaacc aaatggatag accaacaagc gattcaaagc gaagtctcaa tgcacgaaaa
      841 ttgggattac tggcgaacta aacgaggggt gattgtggtc ggcaaactgc cgccggaaca
      901 aggcacaggt ttaaaaactt gggcggaaac catgggttgg tgcttgctaa gtgatgtgca
      961 atcttgcatt gaagccagcc tgccgtatgc cgatatttgg ctctccaacg agactgtcaa
     1021 acagcgttta ttgcaagcgg atatcgtgat tcaattcggc acgcaaattg tcagcaaacg
     1081 ggtgaatgaa tttttaaaag cattccaagg ggaatattgg gtggtggaac aatcgcacga
     1141 ttaccataat ccaaacgctc atcatcaaac ccgttttatt gccaaagcac atcacttttt
     1201 gcgggtacac ccaccacttc gccaaaagcc atggttgctt gagccattgg cattatcgca
     1261 attctgctcc actttcatag agcaacaagt gggtggcagc ttaagtgaag cctcacttgc
     1321 acacaacatt caagatgttt tagcaattaa tggcaactta tttatcggca atagcttatt
     1381 tgttcgattg gttgatgctc tgtgcaaact gccggaaggc taccctgttt acaccaaccg
     1441 tggtgcaagc ggtattgatg gcttaatcgc aacaatggca ggcattgcaa aaggtagcga
     1501 ccgcccgact gtcggtgtgg taggtgatat ttccgcatta cacgatctta actcaattgc
     1561 attgctcacc aaaattaacc agccgaccat tttatttgtg gtgaataaca gcggtggtgc
     1621 gttgtttgat atgttgcctg ttgatagcaa agcgaaagat caatattacc gccttgctca
     1681 caactttgaa tttgctcaag tcgccacaat gttcgggctt gaatatatcc gcccgtttac
     1741 ttgggcagat ctaaaaacca aactcaaaca cgcctatgct cgtaaaggtg ttaccattgt
     1801 ggaaatcaaa gtaaatgaac acgacggcag caatatgtat aaatcattgc taaaacaaat
     1861 ttcacaggcg gaaattgcct aatcacaact gcccctcgcc aatcctgcga ggggtaaaaa
     1921 tgtatcgact atgctttcct atcaatggca cgctcaacaa ggcacacccg tggttttcct
     1981 tcacggcttg ctcggctcac aacaagactg gcaagctgtt ctccaacatt tgcaaaattt
     2041 tccgcaaatt cgaccgctta ccattgacct tccttttcac ggaaatagta agcacattac
     2101 ctgtgccgat tttgccaacc tgcgacaaca actacacgcc acccttgaaa gcctaatcgg
     2161 ctcacgacct ttctatttag tcggctactc actcggtgga cgagcggcac tcgattatgt
     2221 gttaaatatg gagaatccga atctgcaggg cgtggtgtta gaaggggcga atatcggctt
     2281 aagcacagaa aacgaccgcc agattcgccg gcaaaatgac cgccattggg caaaccgttt
     2341 ccgtcaagaa cccatcgaac aagtgcttga agattggtac aaacagcctg tttttgcgga
     2401 tttaagcgat acggaaagag aatgttatat tgatagcaga aagcacaatc aaggagagca
     2461 aattgctcaa atgctggaag ccaccagcct cgccaaacag cctgactaca ccgaaaaact
     2521 caagcagacg gataagaata ttgtcttttt tatcggtgaa aaggatcaga aatttcgcaa
     2581 aatggcatca agccaccaac taccaacaca actgattgct aatgcagggc ataacgctca
     2641 tcgggctaat gctaaaaatt ttgtggagaa attggggtta ttgatactcc actccgacag
     2701 cgacctacgg tcgcaatcgg ttaagcataa cggtgcagct ctgctgcaca aaagccccag
     2761 tggggcttaa tcagccgtag gctaaatata acctagtacg gctcagccgt gctaggtgtt
     2821 agggctcccc ctgcaaccgt tgcaataagc gatccatcgc ccgataacca agtgcttccg
     2881 ctaaatgcag ttgttgaata ttcgcctcgc cggctaaatc agcaatagtc cgagaaactt
     2941 tcaaaatacg gtggtaggct cgtaccgata aaccgagctt tgtcaatgca gtttctaaaa
     3001 atagggcatc ttgtgccgat aattggcaat ctcgctcaat ttctcgggtg gtgagttgag
     3061 aattgatttt acctgctcgc tcaaattgca tttttctggc taaaaacact cgcttgcgaa
     3121 cctgctcggt ggtctctcct cgattctccg tcccactttg caatgctcct tgtggcaaaa
     3181 gtggcacttc tatagagaga tcgaaacggt ctaagaaagg tccggaaagg cggtttaaat
     3241 accgcattac ctgttggggc gaagtgcggt tatgcgtgcc ttgataatgc cctgtcgggc
     3301 ttgggttcat cgctgcaatt aattgaaagc gtgccggaaa ttgtacttta gcgttggctc
     3361 gggaaatcac aatttcgcct gcctccaacg gctgacgtaa cgcatcaagc actttacgtt
     3421 caaattcagg caattcatct aagaacagca caccgttatg ggcaagtgaa atttcccccg
     3481 gtttggggat cgagccactg taaaaactag aaataaatgt cataaaaact agaaatagct
     3541 gtcagattat ttagaaaaca agaaataggt gtcaaattat tatgagaaaa acacatagtt
     3601 ttcaacaggt tagaaaaatt aaacccactt ggatgagtgt atcggggcat attccattta
     3661 gaaacggagt cagtattccc tatgaatcga ctttagagcg tgattttttg atgtatttca
     3721 cttatctacc ttcagtagat gaagtcgtct ctcaaccgac taccctacct ttcgtcaaga
     3781 atgggatcac ttatacctat acaccagatt tcttcttgtc atttacagac ggtcgaaagc
     3841 cgatgttaat cgaggtcaaa ccgaaagcaa aatggcagaa gcactggaaa gaatggaaag
     3901 aaaaatggaa agctgcaata cgtttttgcc aagaaaacgg ttatgtattc catgtgtatg
     3961 atgaagatcg gattcgacat ctagcgcttt tcaatctcaa ctatgttcag cgctataagc
     4021 gaatacaaca tgaacaagaa gatatagatg ctattctagc ccaagtaaag ctgatgggaa
     4081 ataccactat tgactattta ctctctcgat tttttgcagg ttctctatat cgcatgaaag
     4141 gcctacaaat catctatcac ttactggcaa cgaaacaact acattgcaat tggtttctac
     4201 cacttaatga atttaccgag gtatggggga ataatgatga gtaactctgg atttacagaa
     4261 aagcgctatc atcaccgatt agatcgggga cgtattattt tgcaaaaagg taatatttac
     4321 ctaaatagag aagatggaga acagtatgaa ttggttgatt atatggatga accctcccaa
     4381 ctgctcgtac gaaatctcaa cactcgtaca accaaagtcg tcagtattca tcagcttgaa
     4441 aatttcaaaa tgaacgagcg aacggatctt tctgtggatg taaccgctat tagtaatgaa
     4501 tattgggaga aagcccaaca aaaatatgag gcaattaaac cgttattagg gatggatcaa
     4561 catcggcctt acgcagttaa ggctcgtgca gaagatgttg gggtgaaccc tcgtacatta
     4621 taccgttggt tacaagccta taattccata ggatctatcg caggtttagt cgatcaaaaa
     4681 cgtggatggc aacaaggaaa ttcccgttta acgccagagc aagacaagct gatcgtgcag
     4741 gtaatcaatg agttttacct acataaacaa cgcccaacta ctgagcaaac tattagagaa
     4801 attcgccgcc gatgcaaaat tgaaaaagtg gaaagcccaa gcaaagaaac catccgtatg
     4861 cgcattttgc atatttcaga ggaagagcga ttacggaaac gcgggcaacg agaaaaggca
     4921 cgcaacaaat ttaagccgaa gccaaatagc tttcctgatg cggattatcc attgagtgtg
     4981 gtacaaattg accatacacc agttgaccta attattgtgg atagtaagta tcgtaaaccg
     5041 attggccgac cgtttttgac ggttgcgata gatatataca gcagaatgat tgttggttat
     5101 tacctttcac ttgatgcgcc ttctgtaact tcggtggcga tgtgtattgc acgtggtatt
     5161 ttgccgaaag aacgcctact gcttgattta ggtttgcaag gctcagaatg gaatgctttt
     5221 ggctacccag tgaaagtgca tgtggataat ggcccagatt ttcaggcttt ggatttatct
     5281 aaatcttgtt ctgcacacgg aatccatctt gaatttagac cgatggggcg acctgaatat
     5341 ggcggacata ttgagcgtgt tatcggtaca tttatgaaag aagttcacag tttagcagga
     5401 acaacattct ccaacatcaa agagcgtgac tcttacgatt cggaaaaaga ggccattatg
     5461 actctcgatg agtttgaaaa atggttggta cattacattg tgaatgttta ccacaaacgt
     5521 gtgcattctg ctttaggcat ttctcctgaa caaaaatgga agattggtat ttttggagat
     5581 gaaaatgaag tgggatgtgg ttatccgcaa cttcctgttg atgaacaaac actactactt
     5641 gatttcttgc caagtatcac tcgtaccatt cagcataatg gtgtaacaat tgacggactt
     5701 cgttattacg atgttgcgct aaatatgtat atcagtgatt cagatgaaag cggtaaatct
     5761 aaggaatttc tgtttcgccg agacccgcga aatatcagca aaatttggtt ttatgatccg
     5821 aaattaaaac gttacttccc aattccattt gcaaatcagg caatgcccga aatgagcatt
     5881 tgggaatata gagaggttcg tagccgaatt gcgaataaag gtgataaata tatcaacgag
     5941 caacaagttt tagacggttt aaccgagatg cgtgaaatgg ttgctgaatc ggctcaacgg
     6001 acgaaaaaag cacgccgtca ggctgaacga caaaaaatgc acaaagcaag taagccaatt
     6061 attgaaacaa aagtggaaac taaggctgtt gtaccagtcg tagtaacatc gaatttgctt
     6121 gctttagatg atgagagctt atcttttggt gaggtggact aatgcaatac gaacatgttc
     6181 atgaaaaatt ccgccatctt gtgacagcgg acaatcagga gaggattgcg ttcttggacg
     6241 agccaagatg gttaggttat ggtgtcgcca aagacattat ggataatttg gtttctttaa
     6301 tgaataaacc aaaacgccca cgaatgttga atttgttgat tgtcggcgat tccaataatg
     6361 gaaaaaccac actgatccgc cgcttcttcg acttgtacgg tcaggcgtat atcgacagtg
     6421 attctaatgc gatttaccct attcttcttg ccgaagcccc accaagtgcg aatgaaaaag
     6481 aattgtacat ctcattatta gagcggttct acgtaccata taagccgaca gatacgatag
     6541 ccaaacttcg ttatcagact atccatttgt tccgagaatt tcgtgtcaaa atgttggtta
     6601 ttgacgagtt tcattctctg ttggttggta caccacgctt acaacgacag gtgatgaacg
     6661 caattaagat gctttgtaac gagctacaaa ttccaattgt cggtgttggt actcgtgatg
     6721 caattcgggt attacatacg gatcgacaac atgccagtcg ttttgatgtt gcggaattgc
     6781 caatatggaa actagataag gaatttcaaa aactcctatt tcaattccaa gggattttac
     6841 ctttgaggga gagttcaaat ctgcaatctc cagaacttgc tacgaagatc cacaccattt
     6901 caggtgggaa cttagggaac gtgcatcgct tactaacagc ttgtgcagtt caagccatta
     6961 caagtggcac ggaaaaaatc accttagaca tcattgagaa aaattcatgg gtacaaccaa
     7021 caaacggcta tcgcaaaata atcggataaa aaaaggtgtt attcataccc ctatttattc
     7081 aaatgaaagt attagctcgt ggttaattcg tgcatccttt gattgtggta cagatccact
     7141 gacttttaca gggttttatt gggaaaaaca tcggttgtgg acattagatt tagatcgtgg
     7201 cttggaaaat attgagccaa ctatctatga tgatgtacag gcattatcgg gtaaaatcca
     7261 actttcagaa tattccttgt ttcgcaggct ttaccctata aatgcagatg aaaccttagc
     7321 taaaggtcaa gtaagatggg tgattccacg ttcggcaagg aatagaaccc atcacattgg
     7381 tcaacaatat tgtcctttgt gcttggatga gtcgccacat ttacgcaatg attggcggtt
     7441 agcgtggaat tttggttgtc tcaagcataa tatgatgttg gcgaacagat gtttttgttg
     7501 tggtacattg tatcaacccc atttactatc agcggaaaag aggcatttga atcattgtca
     7561 tcaatgtggt gggaaactgg aacaaggcac aatggaaatg agtctagctg aaaagctgac
     7621 attgcaaggt attaatcagg tactcgaaaa agattcaggc atctgttttg gatacaaggt
     7681 gtcgggtaag gtgtggtttg atatgctccg ctactgcatc aacttattga gacgtgcatc
     7741 agacagacca aatcatgcct tagcaaaatt tgtagttaat ctacagattg atgttgaaaa
     7801 tatatgtagc cccaaaacag gactttcttt tgaacaactt ccgctaggtg aacgtacaaa
     7861 tttacttgtg aatgcctatc atattttggg aatggaagca gattctattc gtagtgcaat
     7921 ttatttgtgt ggcggaacac aaaaaacctt tatgtttgaa aaatatcctg atttttggca
     7981 agaatttatg gcactagcac ccgcaggccc aaatagaaca cgaaaacgca ccaagcccca
     8041 aaatgagtct atcacagcaa ttaatcgccg ttgggagcgt ttgaaacgca aactacactt
     8101 aaataacgaa tgaaaattaa tgtaataaaa ccacaggaag aaccttgctc cgcttgtggc
     8161 aaaatttctc gcatttggcg cagatataat ggcgaaggct actgtgccaa ttgctatgct
     8221 aagtggtttc cgaaaaaaga gtgcagctgt tgccatcaac caaagcgtat ctatttgagt
     8281 tcaacagtat gttgggattg cgaaaaacag actgactgtg tacgttgcgg taaggctgcg
     8341 ggtacattca aaattggtaa aattacccgt tatggtgcag tttgtaatgt gtgttcacgt
     8401 tattttcgtg aagaacgaga atgttttgag tgtggtcaac tatctagaac tatgcttcga
     8461 gcatcagagt caggtcatag cgagctactc tgcttgaagt gttttcaaaa atatcggttt
     8521 gcgacttgta aaaactgtcg ccgttatcgc aaagttcatg accgtgataa tcagctttgt
     8581 cgaaaatgta gtgagcaacg aacttcacaa tgcccgaagt gcaaacagga tatgcctgcg
     8641 ggatatggta atatttgtgc agattgttct aggcgaactc tactgttcaa ccttattcga
     8701 atgaatgtgc atttattccg ccacaaaaaa gtgaaaagag catataaaaa gttcattttt
     8761 tggtatctcg caaagtgtgg cgtgagtgtt gtgctgaaaa aatcagacga ccacgtcctc
     8821 ttttttatgc gttgcgatga aaattggggc aacattccag actaccgtga gttggttaca
     8881 ttctttaaac ccaatggatt acggacgcat ttgactgtgt tgcgttggtt gcttgatacc
     8941 aatcaagtca aagtagatga gagcttaaaa gaggaaatgg cggggttgga acgtattcaa
     9001 tccctattta acaaattaag taactctgta ccttgtattg caaactacta cacgcagtta
     9061 caacataaat atgaggcggg caaaacatca ctgaaatctg ttcgattagc tttacaacct
     9121 gcgattgatt tcatcagttc agaatctgtc tcgttactgc cgactcaagt acaactgaat
     9181 agctatcttg tggcaaaaac gggacagatt gctgctatta caggctttat aaaccacctg
     9241 aaccgagaat atcattgtga attagaaatt gaccgctccc tcattcaaaa gatgaaagaa
     9301 aagcagttga agcagcaagc aaaacagcga ttagcgatac tctacaagca agaaaaacta
     9361 agtatcaaag agcaaaatga gctactctca gtcgtattac tgaatttgca caacatttgg
     9421 ataaaatcac cgaaaaaatt aagtatttca gagatagacg gtatagccta ctatgttgat
     9481 aatgagaaaa aatactttat accaaaagac attcacatac gcctgatgga ataagctgtc
     9541 gtcaattttt atttttgcga tctgtgtcga gaaaataata tttatgaatc gacagatgaa
     9601 ataatattga taaagtctct ttgtgtgttc aacaaacagg agataaatat gacattaaag
     9661 gctcaatttc gtgaatggct taaaaaagag caagaacatg atgaaatgat agggttcgcc
     9721 catatccctt gcattttatc taaagtggat agcaaactgg cgattgttag cggaaatgct
     9781 tatgagaaga aatatctcag atattggatc aagtgtttta ggttcgatag aaatatcagc
     9841 aagtatatta aataggttaa atatctagct
//
LOCUS       DANLXQ010000092         9752 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.092, whole genome shotgun sequence.
ACCESSION   DANLXQ010000092 DANLXQ010000000
VERSION     DANLXQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.092"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            64..1542
                     /gene="fruB"
                     /locus_tag="PXD94_001732"
     CDS             64..1542
                     /gene="fruB"
                     /locus_tag="PXD94_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL6286575.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGEGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SPITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAKGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            1556..2500
                     /gene="fruK"
                     /locus_tag="PXD94_001733"
     CDS             1556..2500
                     /gene="fruK"
                     /locus_tag="PXD94_001733"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL6286576.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVASLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRAALDDIIKQTDITVISENH"
     gene            2526..4190
                     /locus_tag="PXD94_001734"
     CDS             2526..4190
                     /locus_tag="PXD94_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL6286577.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAIENYAKAQGWNIKVETRGQVGAGNPISAEEVAAADLV
                     FVAADIDVDLEKFKGKPMYRTSTGLALKKTTQEFEKAFAQAKIYEGGVAKADNAEAPT
                     SEKKGLYKHLMTGVSHMLPLVVAGGLLIAISFMFGIEAFKDETIAGGLPKALMDIGGG
                     AAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIVAGFLAGYTVKFL
                     NGAIQLPASLTSLKPILILPLLGSAIVGLLMIYVINPPVKALMDALVEWLNTMGQTNA
                     IILGIILGAMMCTDMGGPVNKAAYTFGVGLIASQQYMPMAAVMAAGMVPPIGMAIATL
                     IARSKFNTNQRDAGKASFVLGLCFISEGALPFVAADPVRVIASSILGGATAGAISMAL
                     GITLQAPHGGLFVIPFVSQPLMYLAAIAIGSLVTGVVYAAIKQKAE"
     gene            4357..4875
                     /locus_tag="PXD94_001735"
     CDS             4357..4875
                     /locus_tag="PXD94_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL6286578.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQRMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(5028..5891)
                     /gene="rarD"
                     /locus_tag="PXD94_001736"
     CDS             complement(5028..5891)
                     /gene="rarD"
                     /locus_tag="PXD94_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL6286579.1"
                     /translation="MLKGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWVAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
     gene            6069..6653
                     /locus_tag="PXD94_001737"
     CDS             6069..6653
                     /locus_tag="PXD94_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL6286580.1"
                     /translation="MKKIFWILLIAMLAVGGYSLTKYNDLMRAEEDINSVWGNVESAY
                     QRRADLIPNLVNTVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMAKFQEAQQ
                     GVNSALSRLLVSVEKYPELKAHEAFLNLQAQLEGTENRINVERNNFNEKVKEYNKQVR
                     EFPTKLAAMIMGFKAKPQFKSEAGSDKAPTVNFN"
     gene            6735..7193
                     /locus_tag="PXD94_001738"
     CDS             6735..7193
                     /locus_tag="PXD94_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL6286581.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
     gene            7190..8047
                     /locus_tag="PXD94_001739"
     CDS             7190..8047
                     /locus_tag="PXD94_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL6286582.1"
                     /translation="MMKSLCKFLSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANCAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     HGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFDRGSGGFGGGFGGGFGGSRGSGDSFGGGSS
                     GGGGAGGSW"
     gene            complement(8162..8641)
                     /gene="ispF"
                     /locus_tag="PXD94_001740"
     CDS             complement(8162..8641)
                     /gene="ispF"
                     /locus_tag="PXD94_001740"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL6286583.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(8643..9326)
                     /gene="ispD"
                     /locus_tag="PXD94_001741"
     CDS             complement(8643..9326)
                     /gene="ispD"
                     /locus_tag="PXD94_001741"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL6286584.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            complement(9344..9622)
                     /gene="ftsB"
                     /locus_tag="PXD94_001742"
     CDS             complement(9344..9622)
                     /gene="ftsB"
                     /locus_tag="PXD94_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL6286585.1"
                     /translation="MRLLITFFALLLGFFQYSFWFGKNGWSDYQEVQEEIALLKVENT
                     KLTSRNKLIEAEIYDLKNGVDALEERARLEREMVKENEMFYRIVPKNK"
BASE COUNT         2935 a         2097 c         2036 g         2684 t
ORIGIN      
        1 atatagagta aactaaccat aacattacag tttaaaaatc aactttaaat ccaaggagct
       61 aaaatgctta atttatcggc aaaaaacatc cgtttaaatg gttcagcaag caacaaagaa
      121 gaagttatcc gttttgttgc cgccgaatta gtcgccaagg gcaatgtagc agccggctat
      181 gaagtcggta tgcttaatcg tgaaacccaa acctctactt ttttaggtaa cggaattgct
      241 attccccacg gcacgcttga tacccgtgat ttagtcaaag aaaccggcgt gcaaattatc
      301 caacttccgc aaggcgtgga atggggcgaa ggcaacactg cttatgtagt tatcgggatt
      361 gcggctaaat cggacgaaca cttaacctta ctccgtcaac tcaccactgt attaagtgat
      421 gaagaggctg ctgccacatt agcgaaaacg caaaatttag atgaatttat tgccgtatta
      481 agcggtaaga aaaaccttcc tgccttatct caagagctga tttctctgaa tatcgaatct
      541 tcaagcccta tcactttatc agccattaac gccgcaaaac tacaagagca aggctatgtt
      601 tcccaagcat tcatcagcga tgtggttgcc aacaaagcgt tacatttagg caataatcta
      661 tttgtggtgg attccgccaa aggcaaccaa gctaacggta tcgctattgc ccgtaatacg
      721 caaggtcaaa ccctactaac tgtggcagaa accgacaatg ctctacagcc actattaact
      781 caattattaa aaccagaagt aagaagccag tttgctcatt tctctgcgga gcaaatgctt
      841 ggtttattaa aaggcgaaag cgtagcttca acccctgtaa ccaatgtcga agcgagtggc
      901 aaccaagtgg ttggcacatt caccattcgt aacgacaacg gcttacacgc ccgcccggct
      961 gcggtgttag tacaaacggt gaaaccgttc tcctcaaaaa tcagcgttga aaatttagac
     1021 cgcaacactg caccggcaag tgcgaaaagt gcaatgaaag tagtagcact aggggcaagc
     1081 caagggcatc gtttacgctt tgtggcagag ggagaagacg cccaacaagc gattgaggca
     1141 ttacaacaag caattattaa cggcttaggc gaaagtgttt cattcgtacc ggcggtggca
     1201 gatacgattg aaagcattgc tgtttcagca attcctgcgg aagaaactgc gactgctgtc
     1261 gatgccaata cgaaagaagc aacttttgta attaaaaacg aacacggttt acacgctcgc
     1321 ccaagtgcgg tgttggtgaa tgaagtgaaa aaattcaacg ccaaaattga agtgcaaaat
     1381 gttgataaaa attcaccgct tgtaagtgcc aaaagcttaa tgaaaattgt tgcccttggc
     1441 gtaaccaaag gcacgacgct acgctttgtg gcaagcggtg atgatgccga agtagcactt
     1501 gcggcaatcg gtgctgcgat tgaagcaggt ttaggcgaat aagcgaggaa tcattatgcg
     1561 tattgcaacc gttaccctca accctgcctt tgatttagtc ggcaaacttc aacgtattga
     1621 aatcggcgaa gtgaacaccg tagaaaccct tggtttatac cctgccggca aaggtattaa
     1681 cgttgctaaa gttttagctg acttaggcac aaaactttcg gttaccggct ttttaggcga
     1741 agaaaatcaa ggcgatttcg taaatgcctt taaacacaat gatttggctg atgaatttta
     1801 ccgtattaac ggtaaaaccc gcattaatgt caaaatcact gaaaccgagg ctgatgtaac
     1861 tgatctgaac tttctcggct ttgaaatttc ggaaagcgat tgggaaaaat tcgtcacatt
     1921 atctcatcag tgggaaagca aatttgactt agttgcagtg tgtggatctc tccctcgtgg
     1981 cgtaacgccg gaaaaatttg cttcttggtt ggaatcttta caccgccaag gcttaaaagt
     2041 ggtgttagat accagcaatg cggctctcac cgccggctta accgctcacc cttggttagt
     2101 aaaaccaaac cgccgagagc tggaagtgct ggtcgggcat tcactcaact caatcgaaga
     2161 gattattagt gcagctaaac aattacgttc ccgtggtatt gaaaatgtaa ttatatcaat
     2221 gggcgaacaa ggctctcttt ggctaaatca cgaaggcgtg ttacaagccc gcccaccacg
     2281 ttgcgaaaat gtagttagca ctgtaggtgc tggtgattca atggttgcca gcttgatcta
     2341 cggcttatct caaggttgga gcaaagagca gaccttgcgt tttgcctcag ctacttctgc
     2401 tctagctgtt gttcaaagta acgttgggat tgcagaccgt gcggcgttag acgatattat
     2461 taaacaaacc gacattaccg tgatttcaga aaatcattaa acctcaatca aaggaacaaa
     2521 ccaatatgaa tatttctttt gtatttcctc aaacactagg caacgcacgc cgtttcttag
     2581 tgaatgaaat gttatctgcc gctgccaagc aacaaggaca caatgttgtg gcggcaaacc
     2641 aagctgattt tgtggtgtta ttcgataacg caattccaca agaagctgcc ggcaagcaag
     2701 gggcaatttt ggacttagaa caagcctttg ctcaacctga agccagtctc aaacaagcgg
     2761 tcgaaaatgc acaaactttt gcaaatgcta ccgcaaccac tgcggtttca gcggtttcat
     2821 ctggtgtgaa aaatatcgtt gctgttaccg cttgcccaac tggcgtggct catactttta
     2881 tgtctgcgga agcaatcgaa aattatgcca aagcacaggg ttggaacatc aaggttgaaa
     2941 cccgtggtca agtaggagca ggaaatccaa tttccgccga agaagtcgca gcggcagatt
     3001 tagtctttgt agctgccgat attgatgtcg atttggaaaa attcaaaggc aagccgatgt
     3061 atcgcacctc aaccggttta gcattgaaga aaaccactca agaatttgaa aaagcctttg
     3121 ctcaagcgaa aatttacgaa ggtggtgtgg caaaagcgga taatgctgaa gctccaactt
     3181 ctgagaaaaa agggttatat aaacacttga tgaccggtgt atcacatatg ttaccgctgg
     3241 tggtagcagg tggcttgctc atcgctattt cctttatgtt cggaattgaa gcctttaaag
     3301 atgaaaccat cgcaggcggc ttgccgaaag ccttaatgga tattggtggc ggtgcggcat
     3361 tccacttaat gattgcggta ttcgcaggct atgtagcgtt ctcaattgca gaccgtccgg
     3421 ggttagctgt cggtcttatc ggcggtatgc ttgctaccac cgcaggagca gggattttag
     3481 gtggtatcgt agccggtttc ttagccggtt ataccgtaaa attcttaaat ggtgcgattc
     3541 agctaccggc aagtttaacc tcacttaaac ctattttgat tttaccactc ttaggctcag
     3601 cgattgtcgg cttactgatg atttatgtca tcaacccacc ggtgaaagca ttaatggatg
     3661 ccttagtgga atggttaaac acaatggggc aaaccaatgc aattatttta ggtattattc
     3721 tcggtgcgat gatgtgtacc gatatgggtg gtccggtgaa taaagcggct tatacctttg
     3781 gtgtgggttt aattgcttca caacaatata tgccaatggc agcggtgatg gcggcaggta
     3841 tggttccacc aattggtatg gcgattgcga ccctgattgc tcgtagcaaa ttcaacacca
     3901 accaacgtga tgcaggtaaa gcttcattcg tgttaggttt atgctttatt tcagaagggg
     3961 cattaccgtt cgtggcggca gaccctgtgc gtgtaattgc cagctcaatc ttaggcggtg
     4021 caaccgccgg tgcaatttca atggcactag gcattaccct acaagcccca cacggcggtt
     4081 tattcgtgat tccattcgtt tcacaaccgt taatgtactt agctgctatt gccatcggct
     4141 cattagtcac cggcgtagtc tatgccgcta tcaaacaaaa agcagaataa agcacaaaca
     4201 caaatacaaa caaaaacgga gcgaaacgct ccgtttttat atcatattgg tacttactga
     4261 attctatttc atcgaaatat caagtctaaa atattatttt tgcgatcgag atcgaaaaaa
     4321 tcattttcat cactttacga ataatatcaa ctcgttatga ttttgctacg aggagatact
     4381 atgcagttta ttgaatttac cgatcctgat agtggaattt cttatcaata cagtgaattt
     4441 actattgcta atgtcgcatt tatcattaat ttctgctcag atgccgatgt tatatcgact
     4501 ttgtccgcat taggcaaaga tatcacaaac tatataaaca cctattcctg ttgcacaatc
     4561 aaatttatgg cgaaagagca tctagaaaat agcggttcta atattgacat ttatgccccc
     4621 gcagcgaatc atcagttcaa acgtaaagaa ataattgctt tacaagaaac ccttgagcgt
     4681 ttattatttg agcattatgt acgttttaca ccagaaagct atctcttcat tgccgagaga
     4741 gattcactta accgaatgta tcagcgtatg tgtgtgccac gatgtgattt tatgcaatct
     4801 ttccaagtgg tttatccctt gggcgttaat caagattgtt tcattttaat tacgccaaaa
     4861 ggaaatctaa aatgaatcaa caacgtccag tttccgttcg tttaaagcaa gaaggcttaa
     4921 tcgcttttaa gcaagcctat cagcgagcaa aaaaagcagg tctattgaaa ccggtcaaat
     4981 aatctttaaa ccgcttgctc tacaagcgat ttatttttcc ttaattatca caccttgcta
     5041 cgaaaactat cccaactcaa taatccaatc gaaaccagca aacagaccat caaaatatag
     5101 gaattggcat cgagtttttc gccgatggca aaagaaatca gcaacatcac caacggttca
     5161 acatagccca gtaaacctaa tacattaatc ggtaataaat tgctcgagag gatataaaga
     5221 ataaaagcac tgccacttac cagccccagc aatccgaggt agaaataaat ttgcggattt
     5281 tctgccacta ttggcgtcat ttccacttgg ctgagataat aaagtgcaac gggtaatacc
     5341 agtaacaact ccacaaaaaa agtagctaac gtgttgattt gaaaataacg gcgaagggta
     5401 atataaagcg gataacccaa cccagctacc caagtcgccc acgaaatcgt gctgttaaaa
     5461 aacaaactac tgccaacacc gatgactgcg aaaagtagag aaagccattt caggcgggta
     5521 aaatgttctt taaacaccat ttttcctaga gccaccgcca caatcggtaa aagcaagtaa
     5581 ccaaacgaca cctgcaacgc ctgcccgtga ttaggagccc ataaaaacag ccaaagctgc
     5641 acgccggtgt tgagagcaag gagcaaaatg accaatacaa gcggtggatt ttgtcgaatt
     5701 ttttgcaaaa gatccgcaaa agccgattgc tgtttaaaca atataatcgc cagcaaaata
     5761 aacggaatga ctaccactat ccgaaagccg aacatcgcct ctccctctaa cgggcgaagc
     5821 aatatcgcaa aatagtaaac tgctccaaac agcacattgg ctgcaagcga agctaaaatt
     5881 cctttgagca tttggctttc ctcttatcaa caatcaaaca acaagcggtt gactttgaac
     5941 aaaaatttac aaatcttatc tacgccccat agtatgaaaa aatttgtaag taagctagct
     6001 atattcgatt tttcccgata caatgctccc aatttttcac tcttttattt atattatagg
     6061 acttcataat gaaaaagatt ttttggattt tactgatcgc catgcttgct gttggcggtt
     6121 attctttaac aaaatacaac gatttaatgc gtgcagaaga agacatcaac tccgtatggg
     6181 gaaatgttga atcggcttac caacgccgtg cggatttaat tccaaactta gtgaataccg
     6241 taaaaggtca ggctaacttt gagaaagaaa ctttaacctc tgtgattgaa gctcgtgcaa
     6301 aagcaactgc ggtaaccatt gacccaagca atgcaactga agagcaaatg gctaaattcc
     6361 aagaagccca acaaggtgta aactctgctc tttcacgctt attggtttca gttgagaaat
     6421 accctgaatt aaaagcccac gaagcgtttt taaatctaca agctcaatta gaaggtacgg
     6481 aaaaccgcat taacgttgaa cgcaataact ttaacgaaaa agtgaaagag tacaacaagc
     6541 aagtgcgtga attcccgact aaattagcgg caatgattat gggctttaaa gcaaaaccgc
     6601 aatttaaatc tgaagcaggt tctgataaag caccaaccgt aaacttcaac taattttagt
     6661 ttcaatttat tcatattaaa agcaataagc tctttcaaat ccggtgctta ttgctttatt
     6721 tttaagggga acaaatggga ttattttctt ttctctccaa caaattaccg atagatacgc
     6781 aacgcattga acaagcaatt gccgatttag agcaaaaaac ctctgccgaa ttgcgtgtgg
     6841 tggtggaacg aaaagccaaa atcggcaaaa ccgacaacgc cgctatgttg cgagcaagtg
     6901 agcttttcga tgaattaaaa atgcgtgaaa cggctgaaca taacggtgtg ttgatctacc
     6961 tttcattcaa gcctcactac gctgcgatta ttggcgatga aggcattcat caaaaagtcg
     7021 ataacgattt ttggcaaaaa atctatgcca caatgtgttt ggactgccaa caaggcgatt
     7081 acacccaagc catttgtgat gcaatcaaaa tggtggaagc tccgctcgct caccatttcc
     7141 cttatcaggc tgatgataaa aacgagcttt caaacgaggt ggtagtcaaa tgatgaaatc
     7201 cctttgcaaa tttctatcta ttttcaaccg cttgttattg gtgtgcttgg ctctgtttag
     7261 cctaaatgcc acagcggtga cctacccaga gccgcctaat ccgttttatt atattacgga
     7321 ctacaccaaa aacaccttga gccaacaaga atggcggaca ttagaagatg ccttaattgc
     7381 taactgtgcc aagacaagtt cgcaaattgc cgtggtgatt attccaagca cggaaggcga
     7441 ggcagtttca acctatgctc ataccctgtt taacaaatgg ggaatcggac ggagcaaaaa
     7501 taacgagaac aatggtatat tgttattaat tgccaaaaat gatcgcaaac tctttattgc
     7561 caccggacgt gggcttgaag gagcgttacc tgatgcgatt gcatcgagca ttatccgcaa
     7621 tgacatcacg ccattcttca aacaagagca atatgcagcc ggtatcgccc atggcttatc
     7681 cgccattatg gcagccatta acggggaata tgccccttat gcaagttatg gcgaagaaat
     7741 gcaagaattt gatgacatag acggcttact ctttttcttg tttgccggca ttgtgatttt
     7801 cctaattttc cgcccacgag gaaacagtac ctatattagt cccagctcaa tggatcaact
     7861 aggacaaata ctaagagaaa gccaacgcag aggtgggggc tttggcggtg gtctcggagg
     7921 tggctttgat cgaggctcag gtggatttgg cggaggcttc ggtggtggtt ttggcggaag
     7981 cagaggcagt ggcgacagct tcggtggtgg ttcatcaggc ggtggaggcg ctggaggaag
     8041 ctggtagttt ccgcccaatt taaaacaata caagcggtca tatttctcaa aaaaattgcg
     8101 tatttttcgc taaacgcaca aaacgctaat gaaaataaga ccgcttcctc acttctctaa
     8161 gctatgccct ttttagtaac aatgctacgg cttcacaagc tatgccttca cctcgcccgg
     8221 taaaccctaa tttttctgtt gtagtggctt ttacattcac ttgtgatata tcgcattgta
     8281 gatcgtctgc gatcacctgt ctcattttat caatatgcgg tcgcatcttc ggagcttgag
     8341 caataatggt tacatcgacg ttaccgactt gatagccctg ttcttgcact tgtcggtaag
     8401 cttcaatcaa caaaccacga ctatcggcat ttttgtattg catatcagta tcagggaata
     8461 atttgccaat atcacctaaa gctgctgcgc caagcaacgc atctgttaaa gcgtgcagcg
     8521 ctacatctcc gtcactatgc gcaatgaagc ctgtatgata aggcacttca accccagcaa
     8581 tcatcagtgg tctatcttcg ccaaaagcgt gaacatcaaa accgtgtcca atacgtatca
     8641 ttttagttct ccaaattatg tgtaaggtaa aattccgcta aggctaaatc ttcagggcga
     8701 gtgattttga tattatcact tctacctgaa accaattgtg gctggtagcc tgcaagctcc
     8761 attgcagatg cttcgtctgt tacctgaagc ttattggcaa aagcagagga taatgcttgt
     8821 tttaatgctt gagcaggaaa aaattgtggg gttaaagcgt gccataaggt tgagcgatct
     8881 tctgtgtgtg cgattttcgt gccgtttgct cttttcatcg tatctaccgc tggaatggcg
     8941 agaatgccac cttgcggatc gttaatttgc aaaagtttat ctaaatctga ccgcttgagg
     9001 caagggcgag ccgcatcgtg aactaatgcc caagcatttt cagtgttgat ttcctgtaag
     9061 gcatttagca ctgaatctgc tctcgtttct cctccaaaaa caagttggat cttaggggat
     9121 tttaggagtt ccaatgtgga ataaaaaggg tcttttcttg ccaccgctac aatcactttt
     9181 tcaacatgag ggtgttcaag aaaaatagac actgtatgct ctaagatagt cttacccttt
     9241 aattgtaaat attgttttgg tttttctgct tgcatccggc taccaactcc tgacgccgga
     9301 attactgcaa taatttttcg cttcataata tatcaattat gtattatttg ttttttggta
     9361 caattcggta gaacatctcg ttctctttta ccatttctcg ttcaagtctt gctctctctt
     9421 caagtgcatc tacgccgttc tttaaatcgt aaatttcggc ttcaattagt ttattgcgtg
     9481 aagtaagctt tgtgttttct acttttaata aagcaatttc ttcttgcact tcttgataat
     9541 cactccaacc attttttcca aaccaaaagc tatattggaa aaagccgagt aatagggcaa
     9601 aaaaagtaat taataaacgc atttattttc tcttatatga aatagtaatg aactaagcgg
     9661 gttgatttta ccttgaattt gcaaaaaatt ctaaaaatct taccgcttgt tacttaaaca
     9721 ggcgatggat agtgctaaat gtattcgaaa aa
//
LOCUS       DANLXQ010000093         9606 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.093, whole genome shotgun sequence.
ACCESSION   DANLXQ010000093 DANLXQ010000000
VERSION     DANLXQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.093"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..472
                     /locus_tag="PXD94_001743"
     CDS             <1..472
                     /locus_tag="PXD94_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286586.1"
                     /translation="SNQNKLYKEFERIFTSGKIKEKTNLWNNIEQLHFALVQAELKYS
                     GSNIPSEYSKFNLENVLIDFPNKTNSVAYQNLVIHNAKTRAGKLKTQYANAHDLGNLV
                     LVEDIQKIFSGLLEIIYQVRCHLVHGSLSPTPENHEVVKYCYLILWDCLKGFCD"
     gene            complement(740..1477)
                     /locus_tag="PXD94_001744"
     CDS             complement(740..1477)
                     /locus_tag="PXD94_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6286587.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLPP
                     EQERQKVRSVAEQLAISHLLDNTYQQLSGGEQTRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLVSGNTQAVLNAE
                     NIATAFGVKAEMDWEKREMWGIEKLEV"
     gene            complement(1474..2442)
                     /locus_tag="PXD94_001745"
     CDS             complement(1474..2442)
                     /locus_tag="PXD94_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6286588.1"
                     /translation="MKKLNIVLGSLLLLIVSNAAFYQLSDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFKNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWALPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGVPFLIWILFTQQWRLAQ"
     gene            2623..2958
                     /locus_tag="PXD94_001746"
     CDS             2623..2958
                     /locus_tag="PXD94_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6286589.1"
                     /translation="MYHIERTEIFDQWLENLKDPLAVVTIARRIERAESGNFGDTKYI
                     AEGISEMRLNVSKGYRLYYAQKGSIIYILLNGGHKGTQQADIDKAKMLWAEIKQHMAE
                     QEKKNERII"
     gene            2942..3265
                     /locus_tag="PXD94_001747"
     CDS             2942..3265
                     /locus_tag="PXD94_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL6286590.1"
                     /translation="MNELYDAPIPQATREKFGIQPFDAAEYLANDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(3255..5141)
                     /locus_tag="PXD94_001748"
     CDS             complement(3255..5141)
                     /locus_tag="PXD94_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL6286591.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLDLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            5171..5971
                     /gene="metF"
                     /locus_tag="PXD94_001749"
     CDS             5171..5971
                     /gene="metF"
                     /locus_tag="PXD94_001749"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL6286592.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(6020..7180)
                     /locus_tag="PXD94_001750"
     CDS             complement(6020..7180)
                     /locus_tag="PXD94_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286593.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLPLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(7240..8256)
                     /gene="galE"
                     /locus_tag="PXD94_001751"
     CDS             complement(7240..8256)
                     /gene="galE"
                     /locus_tag="PXD94_001751"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL6286594.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHVYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWETERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(8344..8505)
                     /locus_tag="PXD94_001752"
     CDS             complement(8344..8505)
                     /locus_tag="PXD94_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL6286595.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(8483..9484)
                     /gene="lpxK"
                     /locus_tag="PXD94_001753"
     CDS             complement(8483..9484)
                     /gene="lpxK"
                     /locus_tag="PXD94_001753"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL6286596.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PMLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLNQFEPDIIITDDGLQHYALQRDMEWVV
                     VDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQANEHLMTLEPSDAINLVTN
                     QRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQGFADHQAFDKAQLAQFADS
                     QTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACLLAPILQKLGKEDERNTTQ"
     gene            complement(9486..>9606)
                     /locus_tag="PXD94_001754"
     CDS             complement(9486..>9606)
                     /locus_tag="PXD94_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL6286597.1"
                     /translation="LQLIISHKDELLAHKEEIIENQKRELALLRDLVETLKNK"
BASE COUNT         2657 a         2077 c         2125 g         2747 t
ORIGIN      
        1 cagtaatcaa aacaaacttt ataaagagtt tgaaagaata ttcacctctg gaaaaataaa
       61 agaaaaaact aatttatgga ataatattga gcaacttcat tttgcattag ttcaggcaga
      121 gttaaaatat tcaggtagca atattccaag tgaatattct aaatttaact tggaaaatgt
      181 gcttattgat tttcctaaca agacaaattc tgtagcatat cagaatctag ttattcacaa
      241 cgcaaaaacg cgtgcaggaa aattaaaaac acaatatgca aatgctcacg atttaggcaa
      301 tttggtttta gtagaggata tccaaaaaat attttccggg ttattagaaa taatttatca
      361 agttcgttgc catttggtac atggtagctt aagtccaaca cctgaaaacc acgaagttgt
      421 taaatattgc tatttgattt tgtgggattg tttaaaagga ttttgtgatt agttattgtc
      481 aataaagcta caagcggtca attctcccaa aactttgcaa atttccccgc aaaactgacc
      541 gcttgttctc ctctatccat tcacctcaac caacttttct ctctacccct actcaaaatc
      601 ggttaaacaa tctgttgagg taaatgttgt gtcggaatgg gatttaatct taatgaaact
      661 gaaaaaatct tctgccaagc aaatttccct aaaattggaa gttcatttcc aatttctcaa
      721 aaaatcaccc aaaccggaac tacacttcca atttttcaat cccccacatt tcccttttct
      781 cccaatccat ttcagctttc accccaaaag ccgttgcaat attttcggca tttaagaccg
      841 cttgtgtgtt gccgctgacg agtaaattgc cgtttttaag cagaatgatt tcatcacaaa
      901 aacggtaggc aaggggcaaa tgatgaatca ccaccacgca ggtttgtgtt ggagttaggg
      961 ctttgagctg ctgcataata tcgatttggt agtaggggtc gagggcggca atcggttcat
     1021 cggcaagcaa gatcggggct tgtttcatca aacaacgggc gagttgcact cgggtctgtt
     1081 caccgccgga gagctgttgg taagtgttgt ccagcaggtg agaaatcgcc aattgttcgg
     1141 caaccgaacg caccttttga cgctcttgtt caggcggaag cggtgcgggc aagccgagtg
     1201 caatcacatc atacaccgaa agttgccacg ccacgttgag ctgttgggcg aggtaggcaa
     1261 attgtcggct cctctcaacg gcggaaaggg cgtgcagttt gcaattttcc agccaaattt
     1321 caccgcttgt cgggcgtaga atgccggcaa tcgctttgag caacgaggat ttccctgccc
     1381 cgtttgcgcc cataatgccg atcaatttgc cttttggcag atagcaagag aggtgttgaa
     1441 taccgtgggc aagggtgaga ttttcaatct tgatcattgt gccaatctcc attgctgggt
     1501 gaataaaatc caaatcaaaa acggtacgcc caaaattgcc gtgagcgtgc cgatgtaaat
     1561 tcgggcaaaa aacggcacat attgcaccgc cagatctgca atcagcagta acaacgcccc
     1621 gattaaagcg gaggtaagat aaagctggga cggcggtttt ttcagccaaa tacgggcaag
     1681 atgaggggct atcaagccga taaaaccgat ggtgccggtc tgtggaatcg ttgctccgac
     1741 caacagcgcc acgccaatgc taacggtaaa aaaactgcgt tgcaggttga tgcccattgt
     1801 tgctgccgtc tcttcgccaa aggtgagcag gtcgagataa cgccgttccc gatagaggca
     1861 gacaatgccg agcaagatca acggtaacgc ccaacatact gcctctgcgg ttgcccagac
     1921 taatgagcct tgcaaccagc ggtagagttc cgccaacgcc cacgggcttt cggcattgga
     1981 aagcagtaaa gtcaccgctg ccccgagcaa cacgtttacc gccacgccac ttaaaatcat
     2041 cattgttgtg ccgtggcgaa agctaatcag ccacaccagc aaaaatgccc ccagcgcccc
     2101 tgccacaccg ccaaacagta gccagcccat cggggctgaa aaatagtaga gcagaaatac
     2161 gctcaccgcc gttgcccccg aactgctccc aagcaaaccg gggctggcaa gcggattttt
     2221 aaacacgccc tgcatcgcat tgccggcaag cgcaaggctg gcgcccgtca gcaccgccaa
     2281 gccaatacgg ggcagtcgaa tttcacccag caccagccag cgcagatcgc ttagcacgcc
     2341 ttccgcattt ttaagcaagg caaaatcgct cagttggtaa aaggctgcgt tgctaacaat
     2401 cagcaacaac aagcttccaa gcacaatgtt taatttcttc attcttcttt ctctcatcaa
     2461 acgtgcaaat tataccatag cgccaaagtc aagctgaaaa cgctattttt gcgatcatca
     2521 tcgaaaaact agcgtttgcc gaatgtaacc cacgacaggc tttgctatat tccttcaatc
     2581 agcctaagtt ttgtatttaa aacattacag attgggggta gaatgtacca tattgaacgc
     2641 actgaaattt tcgaccaatg gcttgaaaat ttaaaagatc cacttgctgt ggtgaccatt
     2701 gcaagacgta ttgaacgtgc cgaaagcgga aattttggcg ataccaaata tatcgcagaa
     2761 ggaatttccg aaatgaggct aaacgtcagc aaaggttatc gcctttacta tgcgcaaaaa
     2821 ggtagcatta tttatatact actaaatggc ggacataaag gcacgcaaca agctgatatt
     2881 gataaagcta aaatgctatg ggcagaaatt aaacaacata tggcagaaca ggagaaaaag
     2941 aatgaacgaa ttatatgacg ccccaattcc acaagctact cgtgaaaaat ttggcattca
     3001 acctttcgat gccgctgaat acctcgccaa tgacgagcta atcgcccttt atttggcgga
     3061 aacattgaaa gacggcacgg acgaggagtt tattcaagca ttgaataccg tggcacgagc
     3121 gaaaggaatg aatgagttag cgaaaaaagc agggatcggg cgtgaaagtt tgtatcaaag
     3181 cctttccagc agtaagcccc gttttgagac tatccgcaaa attattagcg cattaaattt
     3241 ggagttgtcg gtcgttaagg cgtaagtttg tgcattaatt gtatcccctg ccaaaccccg
     3301 tggtcgaaac agtaggtgta tttcatcggt aggacaagcg gtggattttg gctcattttt
     3361 tgccatattt tttgtaatgc tgggtgtttg agcagttcgg cttggttgtt atagctttgg
     3421 ttatcggtga ggaacaataa ctgtttgggt tgcgctaaca gcagtttttc tagcgagaaa
     3481 ttctgtggcg tgaggtcgct ttttaatggt gacagaccga gaagatccaa caacgtttga
     3541 tattgcggaa aatgcggttc gatcataccc gtttcgctta gaattaaagt ttcagcaagc
     3601 ggttgtttta gcggaatttt ttgcaaacgt agccgttcca ccagcttttc cgctttggct
     3661 tgattttggg ttagctcgcc cagttgtaaa atcagtgtga aaagctgttc gggtgtttgc
     3721 ggactgtcgt ttaatgcgac taacttaaag ccaagctgtt tcagccgtgc ggtgagttgc
     3781 ggatagaagg tttcattcag caaaatggtg ctgtcggcat agggcaacag ggcggtgagt
     3841 tcaggctcaa cggtgggctt gtccgtgttc agtttatcca acatcatcaa cggcttttgg
     3901 gaatagggcg acatcgcccg aatttgctgt ggttcggcaa gttccagcaa cagacggtcg
     3961 ctacacaaat ttggcgataa acggtaagct cctgaaatac gcccagtgat agaattttta
     4021 aactgtgaat tatcggtgta acggccacta atagaaaaac tgtgatcttg ttcggtaaat
     4081 aagcgatatt ctagtgccgc acttttttcg gataaggttt tttcacgcaa atatttatca
     4141 gaggtatagc gtgtttttgc ataatctgtc agtaaactga ccgcttgtgt cacttctcct
     4201 tcacggtcaa agtttacatc taattgataa ttcgcattaa gttgtttttc gttgctgtag
     4261 cgagcatttg aaccgaaatg tgttgcttta ctattgaatt ggctgatatt tgcttggtgt
     4321 ttcagcagct cttgttcatt tccccaataa ccgcctaatt taaaagtttg attgcgagta
     4381 cgggaatatt caccgctaac gtctgaatta tagccatcaa tatgaacggt ttgggaagat
     4441 tgagcggcaa gaacttctac ccctttgtta ccgaggtcat aacctaaacg taaagagccg
     4501 ttatcacgat gaaagccatc tcgttcgctt gcaccaccgg ttttcacttc tgagcctgtt
     4561 tctgtggtat agctgaaatg gtttttactc agtgcggaaa tgcctttggt acgatggcta
     4621 tcaccgtgta aggcatagta gaaaccgttg tgaaatccgg aaagtgttgc gctggcatca
     4681 cgggtattat ttgagcctaa accaagatcg acctccgcat tgaatggttt ctctttgtat
     4741 aaaccgcttt tagtggtgat ataaactaca ccacccatag cactgcttcc ccaaagggcg
     4801 gattgctcgc cacgcaacac ttcgatacgt tcgatattgc taatcggtaa gccaccaaaa
     4861 tcgaaatttc caatattaat cggattgact tttacaccgt caatcacaac cgcagtatgg
     4921 cgagattctg cccctcgtaa aaataagcta gtaagagtac cacgcccacc ttgttgccca
     4981 atcgccacgc ccggcacagt ctttaacaca tcactcacat aagtcgcatt gcgtgcggca
     5041 aaatcttttt cggtaagaac cgtcacgctg gaagcggttt tgtcttggtt gattggggtt
     5101 gcataggcgg aatagactgt tacggaatca agctgagtca tctcattttg tgcaaaagct
     5161 gtacttgcta gtgcaacaga aatggcgaat gctgtgggat tctattgatc gcctaagtcc
     5221 tttaaaaccg aaatttgttt cggtaactta tggagcaaat tctggtgagc gagatcgtac
     5281 acacagtatt gtgaaatcaa tccaagaaaa aacagggatc atttcagctc cgcacttaac
     5341 aggcattgat gcaacaccgg aagaattaaa ggtaattgcc aaagattatt gggatagtgg
     5401 tattcgccga attgttgctt tgcgtggcga tataccgaaa ggttatgata aagcactatt
     5461 ttacgcctca gatttagtcg ctttattaaa atcagtagca gatttcgata tttccgtggc
     5521 tgcctaccct gaagttcacc ctgaagcaaa atcagcacag gcagatttac ttaacttgaa
     5581 acgtaaaatc gatgcgggtg caaaccacgt tattacgcaa ttcttttttg atgtagagag
     5641 ttatttacgt tttcgtgatc gctgtgtttc tattgggatt gattgtgaaa ttgtgccggg
     5701 tattctgccg gtgacaaatt tcaagcagtt gcaaaaaatg gcagcagtga ctaatgttaa
     5761 gatcccgact tggatgggta aaatgtacga agggttagat gatgatcaaa ccaccagaaa
     5821 cttagttgca gcaagtattg caatggatat ggtgaaagtg ctttctcgtg aaggggtgaa
     5881 tgatttccac ttctatacct taaatcgtag tgaactcact tatgcaatgt gccatacttt
     5941 aggcgtaaga cctgatttaa caactgcata atcgaacatt aaggctgtat agctaaatga
     6001 gctatacctc cttatagttt tacttctctt cagccaccca aatctcgcca ttttttaata
     6061 ataaataggc tttgttggaa tcggctttgg tttgtttaat tccagccggc agggcggttg
     6121 gcaattcagt gatcttgcct gttttatcca acacaacaac ttgttgatag cccatttggg
     6181 gtatcagtgc gaaagtctct gttgtcgcca ctaaattggt ttcgtgatca ccatagcggt
     6241 gattgcttaa gccggaggta ataaaacgtt cagctaattg gtttcctttc aggctgtatt
     6301 caaccaaatt gccttttaga tgtggcattt ggattgcata gagcttgcgg ttaaagataa
     6361 acggcgattg ccaacggtta gtcgacagcg gctcactttc ggcttctatt gataattttc
     6421 cgtctttaac gccaatcaca acagtgctgg ccccattgcc atcgccagag ataacagaaa
     6481 cgatcttggt ttttccattt tctgtgaaag tcgcccagcg gttggcttca aatactcgcc
     6541 cttgttgggt gagtttttct gctaaggggt ttagggtatg gcgttctaga aattgaattt
     6601 cgcctgcctc tacatcatcg cctaatacac cgtgttgata tgtaaaatca tcaggctttg
     6661 caagtacagc aatatgccct tggttaggtg tttcggcatc aaaatcaatt tgcagagggt
     6721 gagcatcagg caacacctcg gtttctgcgg tggcagtaat agtggcttgg tttccttgcg
     6781 tttcaattct ggctaactta aattgtccgt tttgtttggt cacggcaatg gtggcaaaat
     6841 taagggcggt cattgtagct ttcggggcaa ggggaatgtt ggaggtatag attttatcag
     6901 ccgtccacag cataaaatgc ccgttttgat ccgctaacgc aattcgttgg tattgagctt
     6961 taggttcaat ctcggtggaa actccatctg ccagtttttg agcttgttgt gtgactctcc
     7021 ataattgttt ttgttggtta attaccacta attcgccttg ttctgaaagg tcaaattggc
     7081 ttactttgcc aatttgggga agtgggagtg gagaaagctg aacatcagca aacaaagaag
     7141 cggagaacaa gcttaagatt aacagaatag attttttcat tagcgttacc taaaaagaag
     7201 atttaaatga taaagggaga tgttatctcc ctttacgctt tagcctttat aaccattcgg
     7261 gttatttttt tgccaattcc aagtgtcttt catcatttgt tctagcccac gctcggtttc
     7321 ccaacccagt tgctctaacg ctttttgtgg agcggagtag caaacggcaa tatcgcccgg
     7381 gcggcgatct accactttgt atggaatagt aatgccattt gcggcttcaa aggcttttac
     7441 catatctagc accgaataac ctgttcctgt gcctaaattg taaacgtgga aaccggcatc
     7501 attttggtgc ttgtctaagg cttttaagtg accaagtgct aaatctacca cgtggatata
     7561 atcacgcaca cctgtgccat cgtgagtatt ataatcgcca ccaaacaccg ataattgagg
     7621 taatttgccc acagccactt ggctgataaa cggcattaag ttatttggga taccgtttgg
     7681 atcttcaccg attaaaccgc tttcgtgagc ccctaccggg ttgaagtaac gcaatactac
     7741 cgcacttaat tgtgggaagg ctttaacggt atcttcaaga atgcgttcaa ccatatattt
     7801 ggacgtaccg taagggttgg ttgtgccacc gacagggcaa gattccacaa tcgggataat
     7861 ctgcggatca ccataaacgg tcgccgatga actaaacaca atggtattca cattggcttt
     7921 tagcatctct tctaccaata caatcgagcc ggttacattg ttttgatagt aacgtagcgg
     7981 ttctctgacg ctttcgccta cagcttttaa accggcaaag tgaattaccg attcaatctg
     8041 attttccgca aaaattttgc gtaaaatatc acgatctaaa atatcgcctt gatagaattt
     8101 tacgctcttg ccggtgatct gcttcacacg ctcaagtgat acttcagagg aattagaaag
     8161 attatctaac accacgattt cacgattttc gtttaataat tcaactaatg tatgtgaacc
     8221 gatgtagcct gcaccacctg taactaaaat tgccatttta tgttcctcta taaaattaat
     8281 acgaaccaag gttattgttg ctattttacc cgatttaatc aggcttgcta atatttattc
     8341 agtttataat gccactccgg cttccggcag taattccgga atgccgtttt taatcgggta
     8401 agcaatattg agcttagggt taattaatcg gttgttctct ttgtcccatt ctaatttttc
     8461 attagagaca gggcagacga ggttattgag tagtgtttcg ttcatcttct tttcctagtt
     8521 tttgtagaat cggggctaac aaacaagcgg tcgatttttc tgaaaatgtt gcagaaaccg
     8581 gtacatacca ccaatttggc tgtgcaaaat cgtggcattt taccgcatct ttctcggtca
     8641 ttaataacgg cgtttggcta tcagcaaatt gagccagctg agccttatca aaggcttgat
     8701 gatcggcaaa gccttgagtt ttggcaagcg gaatgcctaa tttttctaac atcgtaaaaa
     8761 agcgtggtgg gtgaccgata ccggcaatcg ccacacaggg ttgggtaaat tcgcttaatg
     8821 gcttgcgttg attggtgact aaattaattg catcagacgg ttcaagcgtc attaaatgct
     8881 cgttagcttg cggtgtgccg ccgttgcaaa taaccgcttg cacggagttt aaacggctcg
     8941 gtaattcacg caatccgccc gccggcagca caaaaccgtt accgaaacgc cttacgccat
     9001 caaccaccac ccattccata tctcgctgta gggcatagtg ttgcaagcca tcatcggtga
     9061 tgatgatgtc cggctcaaac tgatttaata gtaattcaat gctttgttgg cggtttggag
     9121 aaatagccaa aggcacattg gttcgctgaa caatcagaac cggttcatca cccaccattt
     9181 cagcagaact ttctcgggta actaattgcg gaaacgtttt cgctttaccg ccatagcctc
     9241 gagaaatcac gcctactttt acgccacgct gttgcagttg ttccaccagc caaacgacca
     9301 ctggcgtttt gccgttgcct ccaaccgaga tattaccgac taccagcata ggcaaaggcg
     9361 aacggtacga ttgcaaaata ttcagagaat ataaccgctt gcgagcttga ctaatcagcc
     9421 aaaacagcaa cgaaagcggc gaaagcagcc aagtgataag ggattttgtt tgccagattt
     9481 tcatattatt tatttttaag tgtttcaact aaatctcgta ataaggctaa ttcacgtttt
     9541 tggttttcga taatttcttc tttatgggct aataattcat ctttgtggga gataataagt
     9601 tgtaaa
//
LOCUS       DANLXQ010000094         9557 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.094, whole genome shotgun sequence.
ACCESSION   DANLXQ010000094 DANLXQ010000000
VERSION     DANLXQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.094"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            302..6373
                     /locus_tag="PXD94_001755"
     CDS             302..6373
                     /locus_tag="PXD94_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL6286598.1"
                     /translation="MNKIFKVIFNQTTQKMEVVSELARSQGKAASSTDKRGTIKLAAA
                     SILGGATLISGMAAFMLSATPAEAAVIIDRIEVQQKAPKKVVNGGIDIYTNTSLATDS
                     ITNPYNYFNPGSLSYADRTKLQESNNSVNKEKDSRLLYKSNTTSAIVIGHNAVALDNN
                     RKSRGKPKASGIAIGDYSYSGGGLSIAIGSFSRTASIGSVALGTSARAEGFNSFAAMR
                     QSAAIADYSTAIGSTSWANATASFAIGASATALGDRSFAIGSGTPQTVDGSYGHENRR
                     SIYDGQNNTLAFGTDAFSIGTKAKTNGNHSFAIGTNAWSGGFKLENSNISELGQVINP
                     DTAKVANNAFALGTNTRANSNDTLAFSTNASATGDGSIAFGVNSNTTGIGAIAFGNAS
                     KAWKSNAISIGWNANSHAEKVISIGSHSNASKDGAMAIGDTANSSGNNSVAIGYSANV
                     SKANATAVGTRANITHEGGTAIGFEAKSKGNYATAIAYNSTAKGKNSIAAGVNSTVSG
                     IRAAALGYNNTVSGKDTFVLGSNVTVKTKGSVILGSDSTEATATKEDTATVGTLKYSS
                     FAGDENVVDGDYVSVGNATNARQIKFVAPGKIASNSTDAINGSQLYAVADTLVKTGFN
                     ITADHSGLGGGAKEDNVKLGETVKYTSTDKNVVTTVTNNTIDFGLSDNISVGGKPGKD
                     GSVGVKGADGKDGVTIKPEAIVFHGVDGVNGKDGKDGKASIKVEKGAKGLDGNDGKDG
                     ESKTRIVYEKPNGDKEEVATLNDGLKFGANDGKVHDAKLNTQVDIKGATTNTDWAKFD
                     AGKNIMTQVVGNTITVALAKDLAGLDSATFGTQGDSTVINKDGVTIAKKDPNDATKSN
                     VTLTEDGLSNGGNQITNVDSGLKGKDGQPVKLTEAAGDVLNNAVNVGDLKNTVTETVT
                     EVTTDAFGLKDENNKEFKQNLGTTAKIIGDGNVKTNVVEKDGGHALEVSLVNNITVGK
                     DGEPGKVGIAGKDGKDAIALDGKDGAITVGRPGKDGIDGTIGVNGKDGSAVVLNGKDG
                     SIGLNGTNGKDGLTIKGTEGKPGLVGKDGLPGKDGVTRIQYQPTDGNGDPIGEPEQVA
                     TLNDGLHFKGDDATLVSKKLNETLEIIGGADKDKLTANNIGVNSKDGKLSVQLAKDLK
                     DLNSATFKPQDGENGQEGKGPTTVVNNNGVTITPKDGTDGKAVKLTEDGLDNGGNQIV
                     NVKSGIDGLAGKDGINGKDGKPITSVNDLTDDQLKEVGTNAANINDVVNASNDIIEKG
                     FSIAADNGETDHVKLGETVKYTSKDKNIITTVSDNVIDFTLNNDFTIGGPGKDGSIGV
                     KGADGTVGVTLNGKDGSIGLTGPKGADGKDGASATISVKDGAKGLDGNDGKDGESKTR
                     IVYEKPDGTTEEVATLNDGLRFVGDDGKEIVKKLNETLKISGGDTDLTNLSDNNIGVV
                     NNNGALTVKLAKDIDLTKDGSVTIGDTTVNNDGLTIKDGPSVTKAGINAGDKKIINVQ
                     DGEISATSKDAVNGSQLYQVQEVANAGWNLTANGKDKGNVKPGATVDLNNKDGNIIIT
                     KEGNNVTFGLKNNLTIGGKDGKDGQIGIAGKDGKDGVTIKGDGTITAGRDGKDGVDGS
                     IGATGKDGASVVLNGKDGSIGLTGPKGADGKDGASANIAVKDGAEGVDGTNGKDGLPG
                     ENGKTRIVYETKDKDGKTVTEQVATLNDGLIFSGNNEDVKNRQKLNTEVKVKGEGVDK
                     TASENFKSASGNINVKANGTDTLEVQLAKAIDLTEDGSVTTGDTTVNNNGLTIKDGPS
                     VTKGGIDAKGTKITNVKDGDVTATSKDVVSGSQLYNAMQNTGWYLTVDGNVAETSEKG
                     AQRVNNNGKVAVSGGKNIVVSRKGSTVEVATSSNLEFDSVKVGGTTISSTVAKDGVNE
                     LNIAGSKNAQTRITNVAPGVKGTDAVNVNQLKGAENRLNNRISNVDKDLRAGIAGALA
                     AGNLYHVTQPGKSMVSAGVGAYRGQGAIAVGYSRLSDNGKVGIKFSVNSNSRGDTGAA
                     ASVGYQW"
     gene            complement(6595..7980)
                     /gene="hflX"
                     /locus_tag="PXD94_001756"
     CDS             complement(6595..7980)
                     /gene="hflX"
                     /locus_tag="PXD94_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL6286599.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSSHIKYYVGQGKAEGIAEAVERL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RKTRQKADIPTVSLVGYTNAGKSTLFNAITQAGVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEGVEPHIERNADGTPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKAENCIKTEHFNEFGERIISIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
     gene            complement(8000..8272)
                     /gene="hfq"
                     /locus_tag="PXD94_001757"
     CDS             complement(8000..8272)
                     /gene="hfq"
                     /locus_tag="PXD94_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL6286600.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            complement(8341..9294)
                     /gene="miaA"
                     /locus_tag="PXD94_001758"
     CDS             complement(8341..9294)
                     /gene="miaA"
                     /locus_tag="PXD94_001758"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL6286601.1"
                     /translation="MNKKPLALFLMGPTASGKTDLAIALRQELPVEVISVDSALIYKG
                     MDIGTAKPSKEELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGG
                     TMLYYKALLEGLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDS
                     QRINRALEVFYITGKTMTELTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIE
                     LGFEEEVKRLFLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKR
                     QITWLRGWQGELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
BASE COUNT         2966 a         1679 c         2215 g         2697 t
ORIGIN      
        1 tggttaatta aaaaataggt agaatgttaa taattttctg tttggtattt atttgtaaat
       61 taagtatatt tactgtgtgt ttgtttgatt tataaggggt tttgttaatt ttattatctt
      121 gataataaga gaattgtaaa gaagtgaaaa gcttgtttca cttttgaagt gaaaatctta
      181 ctattatcga tatgactttc ctcttactaa attaagagta gtaaggttgt ttattcggtg
      241 ggttgtaatt gccgaattat ttacgaagta agctttataa taattctaaa ggtataaaat
      301 tatgaacaaa atatttaagg ttatttttaa ccaaacgacg caaaaaatgg aagttgtgtc
      361 tgaacttgct cgatcgcaag gtaaagccgc ttcttctact gataagcgcg gtactattaa
      421 attagcagcg gctagcattc tagggggagc aacccttatc agtggaatgg cagcctttat
      481 gctttctgct acaccggcag aagcggcggt gattattgat agaatagaag tacaacaaaa
      541 agcaccaaaa aaagttgtta atggtggtat agatatttat acaaacacta gtttggctac
      601 agattctatt acgaatccat ataactactt taacccaggt agcttatcct atgcagacag
      661 aactaagctt caagaatcta ataactctgt taataaggag aaggattcta gattattgta
      721 taagtctaat actacctcag caattgtaat tggacacaat gctgtagctt tagacaataa
      781 cagaaaaagt agaggaaaac cgaaagcgtc aggcatagcc attggtgatt attcatattc
      841 tggaggggga ttgtctattg caattggttc tttttctcgt actgcaagta ttggttctgt
      901 agcattaggt acctctgcac gtgcggaagg atttaactca tttgctgcaa tgcgtcaatc
      961 tgctgcaatt gcagattatt ctactgcaat tggttcaaca tcttgggcaa atgctacagc
     1021 tagcttcgcc attggtgctt cggctacagc gctaggtgac cgttcttttg cgattggtag
     1081 tggtactcca cagacagtgg atggaagtta cggtcatgaa aatagacgtt caatttatga
     1141 tgggcaaaat aatactttag catttggaac agatgcattc tcaatcggta ctaaagcgaa
     1201 aactaatggt aaccattcat ttgccattgg tactaacgct tggtccggtg gctttaaatt
     1261 agagaattca aatatttctg aattaggaca ggtaattaat cctgatacag cgaaagtagc
     1321 aaataatgca tttgctttgg gtacgaatac tcgtgcaaat tcgaatgata ctttggcgtt
     1381 ttcaacaaat gcaagtgcaa caggtgatgg ttctatcgcc tttggtgtaa attcgaatac
     1441 aacaggaatc ggtgcgattg cttttggtaa tgcttctaaa gcctggaaaa gtaatgctat
     1501 tagcattggt tggaatgcaa actctcatgc agaaaaagtg atcagtattg gttcgcattc
     1561 taacgcgagc aaagatggtg caatggcaat tggtgatacg gctaattcaa gtggtaataa
     1621 ttctgttgcg ataggttata gtgctaatgt aagtaaggcg aatgcgacag cagtaggtac
     1681 acgtgccaat attacgcatg aaggtggcac agcaattggt tttgaggcca aatcaaaagg
     1741 taattatgca actgctattg cttacaactc aaccgcaaag ggtaaaaact caattgcagc
     1801 cggtgtaaat agtactgttt cgggtattcg tgctgcggct ttaggttata acaatacagt
     1861 ttcaggtaaa gatactttcg tattaggttc taatgttact gttaagacaa agggttctgt
     1921 aattctaggt tctgactcta ctgaagcaac cgcaactaaa gaagatactg caactgttgg
     1981 cacattaaaa tatagcagct ttgctggcga tgaaaatgtt gttgatggtg attatgtgag
     2041 tgtgggtaat gcaaccaatg ctcgccaaat taagtttgtt gcaccgggta aaattgcttc
     2101 caattcaaca gatgcgatta acggttcaca actttacgca gtagctgaca ctttagttaa
     2161 aactggcttt aacattacag cggatcactc tggcttagga ggtggtgcta aagaagacaa
     2221 tgtgaagtta ggtgaaactg ttaagtacac ctcaaccgat aagaatgttg ttacaaccgt
     2281 aacaaataat accatcgatt ttggcttatc cgataacatc tctgttggtg gtaaaccagg
     2341 taaagatggt tcagttggtg ttaaaggtgc tgatggtaaa gatggtgtaa caattaaacc
     2401 agaagccatc gtattccacg gcgttgatgg cgtaaatgga aaagatggaa aagatggtaa
     2461 agcatcaatt aaagttgaga aaggtgctaa aggtcttgac ggcaacgacg gtaaagacgg
     2521 cgaaagcaaa actcgtattg tttacgaaaa accaaatggt gataaagaag aagttgcaac
     2581 acttaacgat ggcttaaaat tcggtgcgaa tgatggcaaa gttcacgatg cgaaattaaa
     2641 tacgcaggtg gatattaaag gtgctacaac aaacactgat tgggctaagt ttgatgctgg
     2701 taaaaatatc atgacacagg ttgttggcaa taccattact gtggcgttag caaaagatct
     2761 agcaggctta gatagtgcaa cattcggcac acaaggtgat tctacagtta tcaataaaga
     2821 tggtgtaacg attgctaaga aagatcctaa tgatgcaact aaatctaatg ttaccttaac
     2881 agaggatggc ttaagcaacg gtggtaacca aatcaccaat gttgatagcg gcttaaaagg
     2941 taaagatggt caacctgtta aacttactga ggcagcaggt gatgttctta acaatgcggt
     3001 taacgtcggt gacttgaaaa atactgttac tgagactgtt actgaggtga caacagatgc
     3061 gtttggtcta aaagatgaga acaacaagga gtttaagcaa aacttaggta cgacagctaa
     3121 gatcattggt gatggcaatg ttaaaacaaa cgttgttgag aaagatggtg gccatgcatt
     3181 agaagttagc ctagtgaata atattactgt tggtaaagat ggtgaaccag gtaaagttgg
     3241 tattgctggt aaagacggta aagatgctat tgctttagat ggtaaagatg gagctatcac
     3301 tgtaggtcgc cctggcaaag atggtattga tggtactatc ggtgtaaacg gtaaagacgg
     3361 ttctgcagtt gttcttaacg gtaaagatgg ttcaatcggc ttgaacggta caaatggtaa
     3421 agatggttta accattaaag gtactgaggg caaaccaggg cttgttggta aagatggttt
     3481 accaggtaaa gatggtgtaa ctcgtatcca atatcagcca acagatggca atggtgatcc
     3541 tataggtgaa ccagagcaag tagctacttt aaatgatggt ttacacttta aaggtgatga
     3601 tgcaacacta gtatctaaga aattgaatga aaccttagaa atcattggtg gagctgataa
     3661 agataagcta actgcaaata acatcggcgt aaacagcaaa gatggtaaat tatctgttca
     3721 attagctaaa gatcttaaag acttgaactc agctacattc aaaccacaag atggagagaa
     3781 tggtcaagaa ggtaaaggtc caacaacagt agtaaataat aatggcgtaa ctatcacgcc
     3841 aaaagatggc acagatggta aagcagttaa gcttactgaa gacggtctag acaatggcgg
     3901 caaccaaatt gttaacgtga agagcggtat tgacggttta gcaggtaaag acggcattaa
     3961 cggtaaagat ggtaagccaa tcacttcagt gaacgactta acagacgatc aattaaaaga
     4021 agttggtact aacgctgcga atattaatga tgttgtaaat gcaagcaacg atattatcga
     4081 gaaaggcttt agcattgcag cggataatgg tgaaactgat cacgttaaac taggtgaaac
     4141 tgttaagtac acctctaaag acaaaaacat catcacaaca gtaagtgata atgtgattga
     4201 ctttacgtta aataacgatt ttaccattgg cggtccaggt aaagatggtt caatcggtgt
     4261 taaaggtgca gatggtacag ttggtgttac tttaaacggt aaagatggct ctatcggctt
     4321 aaccggtccg aaaggcgctg acggtaaaga tggtgcaagt gcaactatct ctgtaaaaga
     4381 cggtgctaaa ggccttgacg gtaacgacgg taaagatggc gaaagcaaaa cccgtatcgt
     4441 ttatgagaaa ccagacggta caactgagga agttgcaaca ctgaacgatg gcttaagatt
     4501 cgtaggtgat gacggtaaag aaatcgttaa gaaacttaat gagacgttaa aaatctcagg
     4561 cggtgatact gatttaacga acctgtccga taacaatatc ggtgttgtaa acaacaatgg
     4621 tgcgttaaca gttaaattag cgaaagacat tgatttaacg aaagatggtt ctgtaactat
     4681 tggtgatact acggtaaata atgatggttt aactatcaaa gatggcccaa gtgtaactaa
     4741 agccggtatc aatgcaggcg ataagaaaat cattaacgtt caagacggtg aaatttctgc
     4801 aaccagcaaa gatgcggtaa atggtagcca gttatatcaa gtgcaagaag ttgctaacgc
     4861 aggttggaac ttaacagcaa atggtaaaga caaaggcaac gttaaaccag gagcaacagt
     4921 tgatctaaac aacaaagacg gtaacattat tattactaaa gaaggtaata acgtgacctt
     4981 cggcttgaaa aacaacttaa ccattggtgg taaagatggt aaagacggtc aaatcggtat
     5041 tgctggcaaa gacggtaaag atggcgtaac cattaaaggt gacggcacaa ttactgcagg
     5101 ccgtgacggt aaagacggtg ttgatggttc aattggagct acaggtaaag atggcgcttc
     5161 tgttgtatta aacggtaaag atggctctat cggcttaacc ggtccgaaag gcgctgacgg
     5221 taaagatggt gcaagtgcaa atattgcagt gaaagatggt gctgaaggtg ttgatggtac
     5281 aaatggtaaa gatggattac cgggtgagaa cggtaagact cgtatcgttt atgagaccaa
     5341 agataaagac ggtaaaactg tcacagaaca agttgcaacc ttaaatgatg gtttaatctt
     5401 ctctggtaac aatgaagatg tgaagaaccg tcagaaactg aatactgaag ttaaagtgaa
     5461 aggtgaaggt gtggataaaa cagcttctga aaactttaag tcagcatcag gcaacatcaa
     5521 tgtgaaagcg aatggtacag acacattaga ggtacaatta gcgaaagcaa ttgatttaac
     5581 ggaagatggt tctgtaacaa ctggcgacac aaccgtaaat aacaatggtt taaccattaa
     5641 agacggccca agtgtaacta aaggcggtat tgatgcgaaa ggcacgaaga tcactaacgt
     5701 taaagatggt gatgtaacgg caacaagtaa agatgttgtg agcggtagcc agttgtataa
     5761 tgcaatgcag aatacaggct ggtatctcac tgttgatggt aatgtagctg aaacttcaga
     5821 gaaaggtgct caacgtgtta ataacaatgg taaagtggcc gtttcaggtg gcaaaaacat
     5881 cgttgtttca cgtaaaggtt caactgttga agtggcaacc tcaagcaatc ttgaatttga
     5941 ttcagtgaaa gttggtggta caactatcag ttcaactgtt gcaaaagatg gcgtaaatga
     6001 gcttaacatt gcaggtagca agaatgcgca aacacgtatt acgaacgttg ctccgggtgt
     6061 gaaaggcact gatgctgtta atgtgaatca gttgaaaggt gcagaaaatc gtctgaacaa
     6121 tcgtatcagt aacgtagata aagacttacg tgcaggtatt gcaggtgcat tagccgcagg
     6181 taatttatat cacgtaacac aacctggtaa atcgatggtt tcagccggtg taggtgctta
     6241 ccgtggtcaa ggagctattg ctgtaggtta ttctcgttta tcagataatg gtaaagtggg
     6301 tatcaaattc agtgttaact ctaacagccg tggtgataca ggtgctgctg caagcgtagg
     6361 ttatcagtgg tagtaactgc ttgattaagc ttttcttccc cacttttggg gaagaataaa
     6421 taaaatcctt caaatagaac ggggaaatct ggtttatttg agggattttt ttattggcta
     6481 aattggattt aatggataaa acaagcggtc aattctttac acttttttgc aaaaaaacgt
     6541 aaagaattga ccgcttgcat aatttaaata taacttactc agaagtgaga ttaattaccc
     6601 ccattttgcc agctcaatat attcggctag atccggaaat tgtttcaccc atttgttcca
     6661 ttgcacttcg ttaatttcaa tagagataat ccgttcaccg aactcattaa aatgctcggt
     6721 tttaatgcag ttttctgctt taaattgtgc gtaaattgca ccggcagtaa ccgggaggag
     6781 caagttttca cacactaagt tactgcgaag cctttcttga atggcttgga ataacagctc
     6841 aatgccgcta ttttcttggg cggaaagata cacggcaact ggcgtgccat cggcattgcg
     6901 ttcaatatgc ggttctacac cttctaattt atcaatttta ttgaatacca ataaagtagg
     6961 aatatctaac gctccgattt cgtccaacac ttgatttacc gcatcaatat tttcgttttt
     7021 gcgttcgtct gagccgtcaa tgacgtggag cagtaaagta gcttcggtgg tttcttgcag
     7081 ggtagattta aaggctgaaa ctaaatcgtg cggtagaaaa cggataaaac ctaccgtatc
     7141 ggcaagaatt gtcgtgccga catcttgcac ctgaatccgg cgtaaagtcg ggtcaagggt
     7201 cgcaaaaagt tgatcggcag cataaacacc agcttgggta atcgcattaa acagggtaga
     7261 tttgccggca ttggtatagc ccactaagga tactgttgga atatcggctt tttgtctggt
     7321 ttttcgattt tgattgcgtt gcttgctgac tttttccaag cggttgagta actgttggat
     7381 tcgcacttta atcaaacggc gatcggtttc gagctgtgtt tcacctggac cacgcaagcc
     7441 aactgctccg cctttttgtt ggtcttgatt agtaactcgg cgaaccaaac gggtcgataa
     7501 atggcgaagt tgtgccagct cgacttgtag tttgccttcg tgcgatcttg cccgttgagc
     7561 gaaaatatcc aaaatcaagc cggttctatc caccacacgg cagccgcata acgattgcaa
     7621 attacgggtc tgagccgggc taagttgatg gttcaccagc actactgttg ccccgagtcg
     7681 ttctaccgct tcggcaattc cctccgcttt gccttgcccc acataatatt tgatatgcga
     7741 actttggcgg ctggtggtaa gcgttgccag cacttcgaca ccggcagatt ctgccagcgt
     7801 tttaaattcc agcaaatttt caacatcttt atgttgcccg agatagagat gcaccaaaat
     7861 ggctttatct tgattttctg gatcggtagg cgaggcggct tctgtagttt ggcttgcttc
     7921 agataaagca aagccaaacg cagaattttc ttctgagttt ggcaatgatt cgtttaacat
     7981 tggttgagta ataatgttat tattccgctt gttcagttac ttcggcagca ggctgagcgt
     8041 gttgggcttg gttgttattg ttatgcgata acgcacgggc aggaacaacc gttgaaatag
     8101 cgtgtttata caccatttgg cttacagtgt tttttaataa aatcacaaat tggtcgaacg
     8161 actcgatctg accttgtaat ttaatgccgt taactaaata aattgaaaca ggaatacgct
     8221 cacgacgaag tgcattcaaa tatggatctt gtaaagattg accttttgcc attgttattt
     8281 tcctttttat agttgtgtga tttttaagac accaaataat gcgataagtg ccaaaggcgt
     8341 ttagatatta tcacaattct ttaaataatg atcgatcttt tccaccattt ttgctttaga
     8401 actggtcgga gctaaactat ctaaccaagt cagttcgcct tgccagccac gcagccaagt
     8461 aatctgccgt tttgccagct ggcgggtggc acaaatgcct ttaaagatcg cctcatcgag
     8521 cgaaatatcg ccttgcaaat attcccacat ttggcggtag cccacacagc gaatcgaggg
     8581 caaattaata tgcaaatctt tgcgtaaaaa taaccgcttg acctcctctt caaagcctag
     8641 ctcaatcatt ttgtgaaagc gttgttcaat ccgttggtgt agaactgcac gatcttgagg
     8701 agcgattgca aattgtaaga tattataagg tagagcctca ccttgctgag cagtgagttc
     8761 ggtcatcgtt ttgccggtaa tgtaaaaaac ttctaacgct cggttgatcc gttggctgtc
     8821 atttgggtta attcttgcac cggcaatcgg gtcgattttc actaattctt gatgcaatgt
     8881 tgcccagccg tgttgtgccg ctcggctttc gatttcagcc cgaatggctg gatcggcaga
     8941 gggcagggga gataaccctt caagcaatgc cttgtaataa agcattgtgc cacccaccaa
     9001 gagcggaatt ttaccctgtg aggtaatatc tgccatttca cgcaacgcat cagcgtggaa
     9061 attcatcgcc gaatagctct cggcagggtc taaaatatca atcaagcgat gcggtgcaag
     9121 ggcaagctct tccttgcttg gctttgccgt gccaatatcc attcctttgt aaattaaggc
     9181 agaatcaaca ctgattacct caaccggcaa ttcttggcgt aaagcaatag ctaaatcggt
     9241 tttcccggag gcagtcggcc ccattaaaaa aagggcaagg ggttttttat tcatattaat
     9301 atactgtata tagatacaaa aaaagccgtg aaattcacag cttttttgtt acaatcctga
     9361 ttgtgctact ggtaggctcg ttagggctat tcgcagtagc ctgagcctaa atcatcctta
     9421 gtagcactaa ggaggcttac aatgaaagaa ctactattga ttttagttgc acttgggttt
     9481 ctgtgtgcag cagttgaaat tttcacgctc tttcaactaa tctatctgtt cttgtaagcg
     9541 gctagatgct aggggcg
//
LOCUS       DANLXQ010000095         9409 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.095, whole genome shotgun sequence.
ACCESSION   DANLXQ010000095 DANLXQ010000000
VERSION     DANLXQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.095"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            177..554
                     /locus_tag="PXD94_001759"
     CDS             177..554
                     /locus_tag="PXD94_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL6286602.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNAEITENPSQLAPIFAE
                     IEKCRAAFPNHYECVFEGREYSLYFDYDEVMAKANNLDAAFDDEEMEEGFQFYNEESI
                     AFCGLDDFEKFLKAYQHFVQTYH"
     gene            643..1230
                     /gene="yihI"
                     /locus_tag="PXD94_001760"
     CDS             643..1230
                     /gene="yihI"
                     /locus_tag="PXD94_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL6286603.1"
                     /translation="MSRTKKTRRITDIMPARKTDKPKQPMPKLGGGKNRKLTRYELDA
                     QAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            1244..1711
                     /locus_tag="PXD94_001761"
     CDS             1244..1711
                     /locus_tag="PXD94_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL6286604.1"
                     /translation="MLRIFLMVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQ
                     AQKDRYIRILDSIEVIARAMISEQCDFSEGVLRLKPLLDVLGRKLSDYTAMWALYGVV
                     ENMPILDERKNLKRNERMKLDLEREAKEAELESDIKNECYQLLKDIEEMRKAI"
     gene            1711..3078
                     /gene="hemN"
                     /locus_tag="PXD94_001762"
     CDS             1711..3078
                     /gene="hemN"
                     /locus_tag="PXD94_001762"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL6286605.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANFDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLKRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALTQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
     gene            3152..5485
                     /gene="lptD"
                     /locus_tag="PXD94_001763"
     CDS             3152..5485
                     /gene="lptD"
                     /locus_tag="PXD94_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL6286606.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIEADSAVINQPRDATYTGDVTIKQGNRSIWADQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGSDATINLLSKDANLANANYQLIGRQGRGTAESGEFGNSKHTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYSIN"
     gene            complement(5627..8266)
                     /gene="ppc"
                     /locus_tag="PXD94_001764"
     CDS             complement(5627..8266)
                     /gene="ppc"
                     /locus_tag="PXD94_001764"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL6286607.1"
                     /translation="MNQQYSTIQHNIHMLGEFLGETIRDAQGNYILELIESIRVLSRN
                     SRAGDENAREQLLDKLANISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGS
                     RSLDSLFLRLKSQNIPVEKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNL
                     LEHDDLTEAESYKIKRRLMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVP
                     EFCRQLNFYLEKHFGVQYPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKAT
                     ELFLQDIKNLAEELSITQCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEG
                     KISTIDESDILTSDQQLWEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRL
                     DIRQESTRHEMAIAEITRYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPET
                     KEVLDTCKIVAEQPEGVISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLG
                     DLDHSEKVMTDLFNIGWYRGVINNYQMIMIGYSDSAKDAGMFAAGWAQYRAQEALVNL
                     CEKYNIELTLFHGRGGTIGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPA
                     VAVESLELYASAILEANIFPPPEPKQQWRDVLAKASDISCQIYRDMVRGELDFVPYFR
                     SATPEQELSRLPLGSRPAKRNPKGGVESLRAIPWIFAWMQNRLMLPAWLGAGQALQQL
                     IDEGNETLLKEMCNEWPFFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLR
                     TQLQADLKTVLALAHDGQLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENA
                     DPVLEQALMITITGIAAGMRNTG"
     gene            8391..9359
                     /locus_tag="PXD94_001765"
     CDS             8391..9359
                     /locus_tag="PXD94_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL6286608.1"
                     /translation="MCLKLLFGFDNLPVVYVLFTVIQVGLSINGFKGIKWLENVSCVF
                     LIGILAYMLYVVNTKFSVEIGERFANIEGTWGMPFWAATTAFLGIYSTMILNASDYSR
                     HVKKGTTPTATGVIYVLSILPVTLFMGLIGLLVTAATGNSDPIEVFATAMDNKFLTVI
                     TLLFIAFAQVTTNVLNNIVPPSYILMDSFKMKWSHATILVGILSICVMPWKLVTAESA
                     DGLSLFIKIYSAFLGPIFAVMVVDYYILRKRTLNVNDLYNKEGIFKGINWAAIIAIAI
                     GSFCSLIVVDLSWYVSLIPSGLSYYILMRVLKSSEAFRKGTVLEHQ"
BASE COUNT         2853 a         1775 c         2034 g         2747 t
ORIGIN      
        1 gtagttcctt tttgatgtat caatactaaa aataacctgt attattctag cagatttctg
       61 ggtaatcgag gagttgataa catttaaaaa ttagcgtttt gcaaaatttt ccaaaaaaag
      121 accgcttgtt aggctataat agccaaccaa acagaccaat tcaaaggaga atcggtatgg
      181 aatatcaatt tacccatagt attcacgggg tggtggcaaa atgttcgatg gatcacgagg
      241 cgtttgctcg ttggttgaat gccgaaataa cggaaaatcc tagccagtta gcccctattt
      301 tcgctgaaat agagaagtgt cgagccgcat ttccgaatca ttatgaatgt gtgtttgaag
      361 ggcgggagta tagtctctat tttgattatg atgaagtgat ggcaaaagca aacaatttag
      421 atgctgcttt tgatgatgag gaaatggaag aaggctttca attttacaat gaagagagca
      481 tcgcattttg tggcttagat gactttgaga aatttttgaa agcctaccaa cattttgtgc
      541 aaacttatca ttgattgaaa tgattaattg aaaccaaagc tattaaggga atctaattcc
      601 ttgtagaata gccgtttata gcaattttaa gatattaaat ttatgagtcg tactaaaaaa
      661 acacgtcgta ttaccgacat tatgccggca agaaagaccg ataaacctaa acagcccatg
      721 cctaaattag gtggggggaa aaatcgcaaa ctgacccgtt atgagttaga tgctcaagcc
      781 cgtgaggaaa aacgtaagcg taaacataaa ggcttgccga caggctcacg caatgctgat
      841 ccggcagagc agaaaaaagc agtggtgaaa gaggtgaaag atccgcgtat cggtagccgt
      901 aaaaaagtgc cgttaatggt ggaatttgtg aaccagccgg aaaaagggcg gacgattaag
      961 gctgtgccgg ttgagccaat caaaccaacg ctttcaccgg aacaagagct ggagcagtta
     1021 gaaaataacg aatgtttaaa tcagttgtta gatgatttgg aggcgggcaa aaccttatcc
     1081 gcagaagatc agaaatttat gaatgaatgt ttagatcgca ttgatgaatt aatgactgag
     1141 cttggcatcg aatatgaaga tgaagagggc gataatggcg atgcgttatt acgccaattt
     1201 gaaacgatgg atattaataa attccgctag gtagccgatt atgatgctaa gaatattcct
     1261 gatggtgctt gccgctttga ttttaatttc gctgggcggg tatgcactgc acttaatgct
     1321 gaaagtgcga gctcagaaaa agcacgaaca agcactgatt gaacaggcaa agcaagcaca
     1381 aaaagatcgt tatattcgga ttttagacag cattgaagtt attgctcggg caatgatttc
     1441 agaacagtgc gatttttcgg aaggcgtttt acgtttaaaa ccgctattag atgttctcgg
     1501 cagaaaatta agcgactata cggcgatgtg ggcgttgtat ggcgtggtag aaaatatgcc
     1561 gattttagat gaacgcaaaa atttaaaacg taacgaaaga atgaagttgg atttagagcg
     1621 ggaagcaaaa gaagcggagc ttgaatcaga catcaaaaat gagtgttacc aattattaaa
     1681 agatattgaa gaaatgagaa aggcaatcta atgtcagaaa ttatttggga tttagccctt
     1741 attcaaaaat ataaccattc agggcctcgt tatacctctt acccaacagc gttggagttt
     1801 aacgaaaatt acaccaatga agattttatt cgtgctgcag ctcgttaccc tgagcgtccg
     1861 ctttcgcttt atgtgcatat tccgttttgc cataaactgt gctatttctg tggctgtaat
     1921 aaagtgatta ctcgtcatcg ccataaagtt gaaatttatt tagattattt agagcgtgaa
     1981 attaaaactc gcgcacctct gtttcaaaat cgtttagtga cccaaataca ctggggtggc
     2041 ggtacgccga cttatttaga cgaagatcaa tcggcacgtt taatgcaaat gctacgagcc
     2101 aattttgatg tgagcgatga tgcggaaatc agcattgaaa tggatccgcg tgaaatcgaa
     2161 ttaagtatgt tggatcacct gcgtgctatc ggttttaacc gtctgagtat gggtattcag
     2221 gatttcgata aagaagtgca acgtttggtg aatcgtgagc aagatgaaga gtttattttt
     2281 gccttgatga aacgggcaaa agaacttggt tttgtttcaa ctaatgtgga tttgatttac
     2341 ggcttgccaa aacagaatgt ggaaagcttt atgtacacct taaaacgtgt ggtagagcta
     2401 aaccctgatc gtatgagcgt atttaactat gcccacttgc caagccgttt tgctgcacaa
     2461 gtgaaaatta aagaagagtt gttgccggct ccggaaacca aattgacgat tttgcaaaat
     2521 tccatcgaat ttttaggcaa aaacggttat aagtttatcg gtatggatca ctttgctaaa
     2581 cctgatgatg agttagctat tgcacaagaa aaaggcattt tacaccgtaa cttccaaggc
     2641 tataccactc aagaagagtg tgatttattg ggaatgggcg tatcggcaat cagcttactc
     2701 ggcgatactt acgcccaaaa ccgcaaagaa ttacaacaat actatgctga tgttgaaacc
     2761 aacggtattg cgttgcataa aggcattgcg ttaacccaag atgattgcat tcgccgtgat
     2821 gtgataaaag ccttgatttg taatttcaaa ttagattttg ccaccattga aaagcaatat
     2881 ggtattgatt ttaaacgtta ttttgccgaa gatttagctt tcttagcgcc gttggcggaa
     2941 gatgaattat taacgatgaa tgacgacagc attgtggttt ccccacgagg gcgtttattg
     3001 atccgtaata tttgcttatg ctttgatgtc tattcaagac aattagcgag aagacagcag
     3061 ttttcccgta ttatttaatg agcaagcggt cgattttggt aaaaattttg caaaaacaga
     3121 ccgcttgttc ccggcttata aaggaaaaat gatgaaacag cgttatagct tactttctct
     3181 tgctgtgatg gcagcttgtt atagcaacag tgccgcagct gatttaaagc aacaatgttt
     3241 acttggcgtg ccgcattttc agggcgaagt ggtagagggc gatcaactta gaatgcctgt
     3301 taccattgag gctgacagtg cggtcattaa ccagcctcgt gatgccacct acaccggtga
     3361 tgtaacgatc aaacaaggta accgttcgat ttgggcggat caggtgcggg ttgagcaaga
     3421 tggcgaaaat gcccgaatgg cgtatttgca gggtaatttt aactaccaag ataatttgat
     3481 tcaggcaacc ggttcagatg cgaccatcaa tctgctttcc aaagatgcga atttagctaa
     3541 tgctaattac caattaattg gcagacaagg gcgtggcacg gcagaatccg gtgaattcgg
     3601 caacagcaaa cacacgctga aaaatgccag tttcacttcg tgcttaccga atgataattc
     3661 gtggcaaatt gaagcgaatg aaatgattca gcacgttgat gaagaatatg cggaaatgtg
     3721 gcacgcccgt tttaaagtgc tgggaatgcc ggtattttat tcgccttact tacaatttcc
     3781 gattggcgac cgccgccgta gtgggttatt aattccaagt tttgagcgtt caagcaaaaa
     3841 cggctggaca tattcgcaac cgttttattg gaatatcgca ccgaatatgg actcgaccat
     3901 cactccaact tactattcac gccgtggttg gcaaatcagc ccggaatttc gttatttaac
     3961 gaaaatcggg caaggcttag tggcaaccga atatatggcg aaagatcgtt taagcgagta
     4021 ccaaccggga aatgatgatc gtaaacgcta tttattccac tggcgtcacg caatgagttt
     4081 tttaaccgat tggcgtttct cggtcgatta caccaaagtg agcgacagac gttatttctc
     4141 tgattttgat tcggattatg gtagcagtac tgatggctat gcgacgcaga attttaaatt
     4201 aggctactat cagccgaatt ataatatctc aatttcaggt aaaaaattcc aaaccttcga
     4261 tgagttggat atcgggcctt atcgcgttct gccacaaatt gattttaact attataaaaa
     4321 cgatctaatt aaaggcggag atttcaaatt ctttgcccaa gcagcccgtt ttgaaaatga
     4381 tagtaaatta atgccaaaag cgtggcgttt ccacgctgag ccaacactga atttcccatt
     4441 agcgaaccgc tatggtagct taaatttaga aaccaagctc tatgcaacct actatcaaca
     4501 aacgaaaggt agtgagagta cggaagaaat taagaaaagt gtaagtcgtg tactgccaca
     4561 gattaagctc gattttaaaa ccgtgttgga atccgataaa cagcttttct ctggctatac
     4621 tcaaacgctt gagcctcggg tgcaatatat ttatcgccca tataaggatc aaagcgatat
     4681 tggctcgaaa gcacatcgaa gtgtgagctt aggctatgac tctgcattat tgcaaaacga
     4741 ctatttctca ctgtttaatg acagacgtta tagcggttta gatcgtattt cgtcagcaaa
     4801 tttagtcacc gcaggtggca caacccgttt ctttaaagag gcaaccggtg aagaggtgtt
     4861 taatttcagc ttaggacaaa cttattatat aagcccgtct aaaattgatg atacctcaga
     4921 taacagcact aaaggtcata cgacttcatg ggcagcggaa tctaactgga aattccataa
     4981 gaattggaat tggcatggta gctatcaata tgatactcgt ttaaaccaaa cggcgttagc
     5041 gaatatgtca ttgcaataca aaccacagtc agacaaattg attcagctta attaccgcta
     5101 tgcaagtaaa gattacatca atcaaaactt gaacacgaat aagtatgggc aatccattaa
     5161 acaagtgggc ggtgtgctag gttgggaatt aaccgataaa gtgtcggtaa tgaccagtta
     5221 ttaccacgac cttgcattga aaaaaccggt ggaagggcaa atttcgctta actacaacac
     5281 ttgctgttgg gcggcaaatg tgtatgtggc tcgtcgctta gtttcaacgc cggtaggttc
     5341 atctgatacg atcaatgatt tctactatga caataaattc ggcattaact ttgagctacg
     5401 ctttggcaca aactacagca atggtgtaac ccgtatgcta aaacgaggta ttattcctta
     5461 caccgagccg tatagcatta attaacaaag taaaaacaaa taagtaaaaa tacaaatcgg
     5521 ggcgaaaaat atttgccccg atttttttat atttggctga taagcggtta aattctgttg
     5581 gaattttgca aatcttccgc taaattcaac cgcttgccat cgaaacttaa cctgtatttc
     5641 gcatacctgc ggcaatacct gtgatggtga tcatcaatgc ttgctctagt acaggatcag
     5701 cattttcacc ttgtttgcga atcctatcta acaattctac ctgaagtaga ttgagcggat
     5761 cggtataaat attgcgtagt gcaatcgatt ctgcgatcca aggtaaatcg gccattaatt
     5821 gtccatcgtg agccagtgca agtacggttt taagatcagc ctgtagttgc gtacgcaaca
     5881 tattgcctaa acggtgtagt tttggctcga ctaggcgttg gtcgtaatat tccgccagcc
     5941 aaatatcagc tttactaaat accatttcca gcattccgat acgggtcgag aaaaacggcc
     6001 attcgttaca catttctttt agtaaggttt cattaccttc atcaatcaac tgttgcaatg
     6061 cttgccctgc acctaaccaa gcaggtagca ttaagcggtt ttgcatccaa gcgaaaatcc
     6121 aaggaattgc acgcaaactc tccactccgc cttttggatt acgttttgca gggcgtgagc
     6181 caagcggcag acgggaaagt tcttgctctg gtgtagcaga gcggaaatac ggtacgaagt
     6241 ctagctcgcc acgcaccata tcacggtaaa tttggcaaga aatatcggag gcttttgcta
     6301 gcacatctcg ccattgctgc tttggctccg gtggtgggaa aatattcgct tctaaaatcg
     6361 cactggcata gagttctaaa ctttcaactg ccacggcagg taagcctaat ttaaagcgga
     6421 tcatttctcc ttgttcggtc actcgtaagc catttttgag tgagcgaggt ggctgagaaa
     6481 gtaaggctgc gtgagccggt gcgccaccac gcccgatagt accaccacga ccgtggaata
     6541 aggttaattc aatattatat ttttcacata aattaaccag agcttcttgc gcccgatatt
     6601 gcgcccagcc ggcagcaaac attccggcat ctttggcgga atcagagtag ccgatcataa
     6661 tcatttggta attgttgatg acgccacgat accagccgat attaaataaa tcggtcatca
     6721 ctttttcact atgatctaaa tcgcctagcg tttcaaataa tggggcaacc ggaatagtaa
     6781 atttgcaccc cgcctctttg agtaaaaggt gaaccgagag tacatcagag gcctctcttg
     6841 ccattgaaat gacataggct gaaatgacac cttcaggttg ctcggcaaca attttacaag
     6901 tgtctaacac ttctttagtt tcagggcttg gagtccagtt ggttggaatc aatgggcggc
     6961 gagaactgag ttcacgcact aagaatgcct gtttatcatc ttccgtccag tgagcataat
     7021 cgcctaagcc gatatagcgg gtaatttctg caatcgccat ttcgtggcgg gtactttctt
     7081 ggcgaatatc taagcgtgat aaacctaaac caaaacagtt tatacgacgt aagcaatcta
     7141 gtaatgcacc atttgcaata attcgcatac cacaggcttg taatgactca tagcaatcaa
     7201 aaagcggttc ccacaattgc tgatctgaag ttaaaatgtc actttcatca atagttgaaa
     7261 ttttaccttc caataattcc gcatagtaaa tttcggtatt aattaatttc tggcgtaata
     7321 cttttagtaa tacgcgatat ggctctaaat ggttgccata tttagcacga aatgcagggg
     7381 tacactgtgt aatcgataat tcttcagcaa gatttttaat atcttgtaag aacaattccg
     7441 tggctttcca acggttcatt cttaatacac ggcgagttgt ttctgccgtg acgaacgggt
     7501 taccgtcacg gtctccgccc atccaagaag aaaaacggac aggggctaaa tttaccggat
     7561 attgcacacc aaagtgtttt tctaaataga agttaagctg acggcaaaat tccggcacgg
     7621 ctttccataa actattttca atgactgcca taccgccttt ggcttcatca acaggggttg
     7681 ggcgttgggt tctgatttca ttagtatgcc aagcaagggc aatcagttgc attaaacggc
     7741 gcttgatttt atagctttct gcctcagtta aatcatcatg ttccaacaaa ttcaaacatt
     7801 tattgatttc aacgtgttta tgccccagcg aacggcgggt tacttccgtt gggtgagctg
     7861 ttagcactaa atcaatcatt aggctttcca cggttttaat cactttttca acggggatat
     7921 tttgtgattt tagacgaagg aacagagagt ctaatgagcg actaccaagt gattcatcaa
     7981 tatgatggcg agaaacagtt tgatgttgtt cagcaatatt tgttagattt agaaaatggc
     8041 taaatgcacg tgcaacgggt aagacatcat cgttagaaat attggctaat ttatctaata
     8101 gctgttcacg ggcgttttca tcgccggcac gagaattgcg agataacaca cgaatgcttt
     8161 cgatgagttc tagaatgtaa tttccttgcg catcacgaat cgtttctccc aaaaattcac
     8221 ctaacatatg gatattatgc tgaattgttg aatattgctg gttcataaaa aattcctatg
     8281 gtaaacataa attaaattag gtctaaatat gccgaaaatc gctgaaaata gcaaatagat
     8341 ttaatttaat ttttgaatta aatgataaaa cgcaaaaaat agctgtcaat atgtgtttaa
     8401 aactgttgtt cggttttgat aacttgcctg ttgtttatgt gctattcact gttattcaag
     8461 ttggcttatc cattaatggt tttaaaggta ttaaatggtt ggaaaatgtt tcttgtgtgt
     8521 tcctcatcgg tattcttgct tatatgcttt atgttgtgaa tacaaaattc tctgtagaaa
     8581 taggcgagcg ctttgccaat attgaaggca cttggggaat gccattctgg gctgcaacta
     8641 cagcattctt gggtatttat tcaacaatga ttttaaatgc ttccgattat tctcgccacg
     8701 ttaaaaaagg aacaacccca acagcaacag gtgtgattta tgtattatca atcctccctg
     8761 taaccttatt tatgggttta atagggttgt tagttacggc agctacaggc aatagtgacc
     8821 caattgaagt atttgcaact gcaatggata ataaattttt aacagtaata acgttattat
     8881 tcattgcttt tgcacaggtt acgacaaacg tgttgaataa tattgttccg ccatcttaca
     8941 ttttaatgga ttcatttaaa atgaaatggt cgcatgccac cattttagtg ggcattcttt
     9001 ctatatgtgt aatgccttgg aaattggtaa ctgctgaatc agcagatgga ttatctttgt
     9061 ttattaaaat ttactcggca ttcttaggcc ctatttttgc ggtaatggtc gtcgattact
     9121 acatcttaag aaaacgtact ttaaacgtaa atgacttata taataaagaa ggtattttca
     9181 aagggattaa ctgggcagca attattgcga ttgctatcgg ttcgttttgc tcactgattg
     9241 tggtggatct ctcttggtat gtcagtttaa ttccatcagg cttgagctac tatattttga
     9301 tgagggtgtt aaaaagttcc gaagctttcc gtaaagggac agtgctagaa catcaataag
     9361 atgacaagcg gtaaaattta ttaaaaatat ttcaaactta agtgagcat
//
LOCUS       DANLXQ010000096         9360 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.096, whole genome shotgun sequence.
ACCESSION   DANLXQ010000096 DANLXQ010000000
VERSION     DANLXQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.096"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..466)
                     /locus_tag="PXD94_001766"
     CDS             complement(<1..466)
                     /locus_tag="PXD94_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL6286609.1"
                     /translation="MHLNFKKIDNSHIALFYNVLVATFFVTVLTFKKGYSYVPMALGI
                     IATLSFLFYRFKLKQKWLLDKEDKFFIFSLIGYFLTFAMSAIFNGDGIREIDNPIQIL
                     LLIPLIILFRLYPIKKETVLHFIPIGALVAGCIAIYQRFILNLKFPFPEIMRI"
     gene            608..1204
                     /locus_tag="PXD94_001767"
     CDS             608..1204
                     /locus_tag="PXD94_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL6286610.1"
                     /translation="MSLLPMNEITSLNQLDLQKSYSYADYLLWKFKERVEIIKGKIVA
                     MSPAPTRFHQRISMKMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
     gene            1352..1567
                     /gene="rpsU"
                     /locus_tag="PXD94_001768"
     CDS             1352..1567
                     /gene="rpsU"
                     /locus_tag="PXD94_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL6286611.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            1721..3469
                     /gene="dnaG"
                     /locus_tag="PXD94_001769"
     CDS             1721..3469
                     /gene="dnaG"
                     /locus_tag="PXD94_001769"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL6286612.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKTPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSEEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMLPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLSDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYFVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            3728..5599
                     /gene="rpoD"
                     /locus_tag="PXD94_001770"
     CDS             3728..5599
                     /gene="rpoD"
                     /locus_tag="PXD94_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL6286613.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDADDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDENGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVPKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAAPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKEPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            5693..6679
                     /locus_tag="PXD94_001771"
     CDS             5693..6679
                     /locus_tag="PXD94_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL6286614.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(6755..7663)
                     /locus_tag="PXD94_001772"
     CDS             complement(6755..7663)
                     /locus_tag="PXD94_001772"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL6286615.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVIGGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            7843..8580
                     /gene="tsaA"
                     /locus_tag="PXD94_001773"
     CDS             7843..8580
                     /gene="tsaA"
                     /locus_tag="PXD94_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL6286616.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            8638..9357
                     /locus_tag="PXD94_001774"
     CDS             8638..9357
                     /locus_tag="PXD94_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL6286617.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGNSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
BASE COUNT         2978 a         1739 c         2092 g         2551 t
ORIGIN      
        1 gaatacgcat tatttcaggg aaaggaaatt taagatttaa tataaacctc tgatatattg
       61 ctatacaacc agctaccaac gctccaatcg gtataaagtg caatacagtt tcttttttaa
      121 tcgggtaaag tctaaagagg atgattaaag ggataagcaa taaaatctgg ataggattat
      181 caatttcacg aatgccatcg ccattaaaaa tagcagacat tgcaaacgtt agaaaatagc
      241 caataagcga gaaaataaaa aatttatcct ctttatcaag taaccacttt tgttttaatt
      301 tgaaacgata aaataagaaa ctaagtgtag cgataatacc taatgccatt ggcacataac
      361 tataaccttt cttaaaggtt aaaacagtga caaaaaatgt ggcgactagt acattataga
      421 aaagagcaat atgtgagttg tctatttttt tgaagttaag gtgcatcgac atatcctaaa
      481 acaatgaaaa ttgctgtcat tttatactaa gggtataaag gttatctatc tctttttagc
      541 gaaagttttg ttgaatttga gggattattc ggcataatat ctttatattt ctgataaagg
      601 aggcggtatg agcttattac caatgaacga aattaccagc ttaaatcaac tggacttaca
      661 aaaaagttac agttacgctg attatttgct atggaaattt aaagagcggg ttgagattat
      721 caaagggaaa attgtggcga tgtctccggc tcctactcgt tttcatcagc gaatttctat
      781 gaagatgaca gcaaaatttt tagaggtttt tgacaatcac caatgccaga tttattctgc
      841 gccttttgac gttcggtttc ccgatgaaaa cggaaaagtc aaaactgtag tgcagcctga
      901 tctttgtgta atttgtgatg tcagtaagtt agatgaaaga ggctgtgtag gggcaccgga
      961 tttagtggtg gaaatccttt ctccgggtaa taccaaaagg gagatgaaag ataaatacga
     1021 gctttaccag gaacaagggg tgaaagagta ttggattgtt tctcccgaaa cccgcactat
     1081 tcagattttt gtgttggaaa acggtaaata tatcggtatt cagccggtgg cagaagatga
     1141 accggcaact tcggtggtct ttccggcatt aagtttttcg accgagaaat tattcgaact
     1201 gtagaaaaat gcaacataat acggcggttt tatgccacaa tactacttta cttatgtgag
     1261 tgcttaaggt agaatgcact accttttttg ttctatgccg ccaaaattgt gtgcggttta
     1321 atttaatcat atttttctga ggtgatggca tatgccagta attaaagttc gtgaaaatga
     1381 atcatttgac gtagcattac gtcgtttcaa acgctcttgc gaaaaagcgg gtatcttagc
     1441 tgaagttcgt gctcgtgaat tctacgaaaa accaactaca attcgtaaac gtgaaaaagc
     1501 atcacttgct aaacgtcacg ctaaacgtaa tttccgtgag aacgcacgta acacacgttt
     1561 atactaatta gttaggctgg ttagcaaact atatcgaaac cgtgattccg ttaggctcac
     1621 ggttttggtg tttttaacgt ttctatttca agcggttgtt ttttgcaaaa aatttgcaaa
     1681 attgctccgc ttgtatcatt caaagagcag gaggaaatca atgaaaggca caattccacg
     1741 ttcatttatt gatgatctta tcgctcgcac cgatattgtt gaggtcatca acagtcgggt
     1801 caaactcaaa aaggctgggc gagattatca agcctgctgt ccttttcatc acgagaaaac
     1861 cccttccttt tcggtaagcc aatcaaaaca gttttaccat tgtttcggct gtggcgtaca
     1921 cggcaatgtc atcacctttt tgatggatta tgacaaactc gaatttccgg aagcgattga
     1981 agaactggca gctatgcacg ggctggaagt accgagagag aatgtgattc aacgtgacgg
     2041 caagcctcaa gctaatttta aaactaagcg taatctttat gaattgatgg aagcggtggc
     2101 gaagttctac cagcagaatt tatcgcacca gattgagcca caaagctatt tacagcaacg
     2161 aggtttgtct gaagagatta tcgaacgctt tgaaattggt tttgctccga atgcctttga
     2221 tgcagcccta cgcacttttg ggcaatcaca agaagatatt cagaaattgc tcgatacggg
     2281 cgttttaacc aaaaatgata gcggtcgaat ttacgataaa tttcgcaatc gggtaatgtt
     2341 accgattcga gataagcgtg gacgggtaat tgcctttggt gggcgggtgt tgccgaatga
     2401 tgagcaaggg gcgaaatata tgaactcgcc cgaaacggtg atttatcata aaggcagtga
     2461 gttatatggc ttgtatcaag cactgaaagt caatgaaacg ccggattatc tgttcgtggt
     2521 ggaaggctat atggacgtgg tcgcccttgc ccaattcggc gtggacaatg cagtcgcctc
     2581 tttaggcacg gcaactaccg gtgaacaaat tcagcaaatg tttcgttgca ccgagcaaat
     2641 tgtctgttgt tatgatggcg atagagccgg tcgagatgca gcttggcggg cgttagaaaa
     2701 tgcgttgcct tatttgcaag acggtcgcca actgaaattt attttcttgc ccgatggcga
     2761 agacccggac acctttgttc gttctcaagg taaagaagga tttgagcagt acgtggcaca
     2821 gaatgccaag ccattaagcg atttcttatt tgacagtttg ctggcggatg tggatctctc
     2881 ttcaaaagag ggaaaatcca agctcgctgc tttagctgtg ccgttaatta atcgtattcc
     2941 gggcgagatg ctgcgagtgt atttacgcaa tattttagga cagaaattag gcattttaga
     3001 cccaacgcag atagaagctc tgttaccgac gaaaaaagcg gcagagaagc ctttacatca
     3061 agcggtcaaa atgaaacgta cgccaatgcg gctgttgatt gcgttattga ttcaaaatcc
     3121 gtattttgtg aaattcgtac ccgatgtaag tgctttagcc tccttgaaag atcctggctt
     3181 tacattatta accgatattg tcgcaacttg tcagcaaaat gcaggtatca gtatgggcgg
     3241 gctacttgaa cattatcggg aagatcccca atataagatt gttgaagccc tcgcaaattg
     3301 ggatcattta gagttacccg aaaatatcga acgaacattt attgacactc tcgattttct
     3361 ctacgccaaa ttagtggaat tacgcaaaga agagctaatt gccaaagacc gaaccgtggg
     3421 cttaaccgat gatgagaaaa aagagtatgc tcttttgatg tttcagtaga attttaagct
     3481 cgaataattc ccctctttag caaagagggg ttaggggaga tttggcagaa gtgatgaacg
     3541 aagaaaataa ttaattctat ctaaaaagaa agttatttat ctctcttgct acacctctct
     3601 gttctcataa gcatcaaagt aatgtgtaaa ttagaaaaat agaatttttt tgtgaattta
     3661 tgaagtcgaa ccaaatttag tggtataatc cactactatt tttgctcgca taataaacgg
     3721 atagactatg gaaaaccaag cagatcaaca attagaacag caatctcaat tagaacttct
     3781 gatcagtcaa gggcgtgaac agggatattt aacgctttct gaagtgcacg accacttacc
     3841 ggaagagttg gtggatgcgg agcagatcga agatattatt cagatgatca acgatatggg
     3901 tatccaagtt ttagaggctg cccctgatgc ggatgattta ctactcgctg aaaatatcgc
     3961 cgatgaagat gtggtagaag aagccaccaa agtgctttca accgtagaat cagagttagg
     4021 tagaactact gaccctgtgc gtatgtatat gcgtgagatg gggacagttg agcttttaac
     4081 ccgtgaaggt gagattgata tcgctaaacg cattgaagaa gggattaacg aagttcaatg
     4141 tgcgattgcg gaatatccgg aagccttaag tggcctgtta gcctgctatg ctcaagtcga
     4201 aaccggtgaa atgcgccttt ctgatttagt gacagcattt gtcgatccta atattttaga
     4261 agaagcaccg ttagaggata ttgaggctgc tttaggtgaa gatgaagaat cttctgatgt
     4321 gcctgatgta gatgacgaaa acgaagatga aaacggtgat agcagttcag acagcagtga
     4381 tgacagctca attgacccgg aacttgcccg tgaaaaattt gaagccctac gcagccaaca
     4441 tgaaaaagtg ttattggtca ttcaaaaaca cgggcgaacc agtaaaaaat cgaaagagca
     4501 aattgaaatt ttatctcata ttttccgtga attccgtttg gtgccgaagc aatttgactt
     4561 attggttgct tctatgcaaa aactgatgaa gcaggttcgc actgaagaac gtcaaattca
     4621 acgctatgca gtggaatatg ccggcatgaa aaaagcggat ttcttgaaag cattccaagg
     4681 gcacgaatta agcgaagcgt ggattgaaaa agcgattaat gccaagaaaa atgcagcacc
     4741 gaaattagct aattatgtgg ataatatccg tgtcaatatt gctaacttac agaatttaga
     4801 gcaaacatca ggcttaacca ttgctcaaat tcgtgaaatt ggcgggcgta tttccaatgg
     4861 tgagttaaaa gcccgccgtg cgaaaaaaga gatggtggaa gctaacttac gtttggtgat
     4921 ttcgattgcg aaaaaataca ctaaccgtgg cttgcagttc ttagatttaa tccaagaagg
     4981 aaacatcggt ttaatgaaag cggtggataa atttgaatac cgccgtggtt ataaattctc
     5041 gacttatgca acttggtgga ttcgtcaagc gattacccgt tcgattgctg accaagcccg
     5101 tacaattcgt attccggtgc atatgattga aactatcaac aaactcaacc gtatttcacg
     5161 ccaatgttta caagaaatgg ggcgtgaggc aacgccggaa gaactggcag aacgtatggg
     5221 aatgccggaa gataaaatcc gcaaagtatt gaaaattgcc aaagagccga tttcaatgga
     5281 aacaccaatc ggtgacgatg acgattcaca tttaggtgat ttcattcaag acgaaagcct
     5341 tgaattacca ttggattccg ccactgcgga gagcctgcgt atggcaacga acgaagtgtt
     5401 agagggctta accccgcgtg aagcgaaagt gttgcgtatg cgttttggta ttgatatgaa
     5461 cacagaccac acactcgaag aagttggtaa acaatttgac gtaactcgtg aacgtatccg
     5521 ccaaattgag gcgaaagcac tgcgcaaact tcgccacccg agccgttcgg aaaccttacg
     5581 cagtttctta gacgactaat catttcccct atgcctgatg gtatagggga ttttgtttta
     5641 atctgtataa caagcggtga tatttaccgt attttttaca aaagagggag agatgaaaaa
     5701 atcactatta gcacttttaa ttggctcagt atttctgatt tcagcttgtg aagacggcaa
     5761 aatgacccaa acggtattag aagccgaaaa acaaattgtt cagttaggca cagaattaaa
     5821 aaatgctcag gctgatttag caaaaaaaga ggctgaagtt gcagaacttt caggggtgaa
     5881 agcagaaaat gaaaaattaa aatcagagtt agagaacgct cagcaaaaca cagcgttaaa
     5941 tgttgaaatt gtgaaaatct ttgataagaa agaggtgatt aagcaccaag ttgatccgaa
     6001 acaagaattt gcaatagaag aaagtacagt aagtgcgttt gtgtctctac caaaaaccaa
     6061 ttttgattgg cttaaccagc ttttgattaa tcaggcctat gattacaatg aagaacaagg
     6121 cagaaagctg aaaaatccaa ccgaagaaga gtttaaaaag catcgtgaag cggtttatca
     6181 agggttggta gaaagtgtga aaaccgagcc agctattggc tatgatgatc atatttccag
     6241 tgcttttatc gggcaacgca ataatattgc tacattcact atgatgcatt acacctatat
     6301 gggtggggct cacggtatgc actatacgca atatatcaat gtagatttag ctaaaaaagc
     6361 aatcattggc ttaaatgatt taatcagccc aaaaaatcaa gttcaactga aagacattct
     6421 ttgggaaaat tacagtagcg tgcgtgtgga tgaaaatggc aactataatg gatttgctga
     6481 taaaaaagag ttccgtattt ctgaccaatt ctactttacc cctcacggca ttgcttttgt
     6541 ttatccgcca tacgaattag gtgcttatgc agaaggcgat atagaggttg aagcggattg
     6601 gtacattatt aataagttgc tcaattcgga gtatcaacgt ggcgaaaaag atggattctt
     6661 tgaagaacta gataactaat tgtaaattta gataaaaaac aaccgcttgt aatcctttat
     6721 tacaagcggt ttagttttgt taaaattttg caacttaccc taacacaatg cccaaactaa
     6781 ataatagatt agtgagcaac gccagccctg ccatttgccc tagaattggg tagagttctt
     6841 ttgggtcttt atgctgatag acaaaacgag cgtgttttgc cagtaacggt acagacagca
     6901 aaaaaatgaa gctataccaa tgttcaaatt cagaaattgc aaaaatcaga taagaaatga
     6961 ccgctagcac taacaatgca acgtggtatt ttcgcccgtt ttggctaccg attcgtacaa
     7021 tgagagtgtt tttcccagct tgtcggtctt ggttaatatc acgcaagtta ttgatattta
     7081 acactgctac agacagcaat ccgcaaccga aagcagggag cagaatcatt ataggtaggc
     7141 tatgagcctg caagtagaac acgccgatta ccgctacaaa gccaaagaaa atcagcacaa
     7201 atagatcgcc cagccccaaa tagccgtagg cttttttgcc gacggtgtaa gtaatggcgg
     7261 caataattga gacgatgcca agcccgataa agaaaaacag atcctgcact gtttcaaaag
     7321 cataggcact taaggcaaca ccggaaacaa acgagagtag gcttaagata atcacggctt
     7381 tcttaagttg ttcaccgcca ataacaccgt gttgaatcgc ccgtagcggt ccgattcgtt
     7441 ctttggtgtc agagcctttg acgtggtcgc cgtaatcatt ggcgaagtta gacaggatct
     7501 gcaacaaaat ggtggtgata aatgcgagta aggtggtaac aacatcaaat ttccctgccc
     7561 aatgagcaag tgctgaaccc acaataattg aagctaacgc cagcggtaaa gttttcgggc
     7621 gagcggtggt aaaccaaatc gaaaagagag aatttttgtt catagagaat aatcttagcg
     7681 taatttcatt tacaatgatg ttaaggattt aagcgatagt gcaagcgtcc actcttgtgt
     7741 taaatggtaa aacgaattgg tacaagcgtg gacgcttgca ccatcctcaa tttcatatca
     7801 ttatgtccaa tattttatat tattttaaca gaaaaaagcg taatgaactc tacctcaaat
     7861 caactaaccc cgattcaatt aaaccctatc ggttttgtgg aaagccccta tgatgaaaaa
     7921 ttttccgtgc ctcgccagcc gaatttggta gcggagggga aaggcattct acgtttaatt
     7981 ccgccttaca atacccccga tgcagtcaga ggcttggaga aatttagtca tatttggttg
     8041 attttccaat tccatcacat tcccgaaaga gagtggcacg ccaccgtcag accgcctcgc
     8101 ttgggcggga acgaacgggt cggggtcttt gccagccgtg ccacccatcg tccgaatccg
     8161 cttggcttat cgaaagtgaa gttagaagcg gtagtggcgg gggggagtga agtttatttg
     8221 aaattaggca gcgtggattt agttaacggc acaccaattt ttgacatcaa gccttacatt
     8281 gcatttgcag atagcgagcc gcaagcggtt tccggttttg ctcagcaaaa accacaagca
     8341 agattagcgg tgcgttttag cgaggaggca aaacaagctg tcgaattttg ccgaaatttt
     8401 gcaaaatttg gcatcgaaca gccgcttagt tttcttgccg atgtcatcgc ccaagaccct
     8461 cgcccggcgt atcaacaagg gaaacctagt gagcgagttt atggtatgaa ccttgccggc
     8521 tacaatattc gctggcaaat tgaaaatgat acacaggcga ttgtgctttc gattgaatga
     8581 actttcctac gcaagtgaga tctaagaaat gatttttaac ccttttaagg actgattatg
     8641 aaattgaaaa attttcttac cattctaccg cttgcagtcg caacagcggt aacttcagcg
     8701 gaaactcata ccgtggaaca taaagataaa ccggcttctt ttaatttctc aaccgaagtc
     8761 tctcgcacgg tggaaaagga cgtgatgcag gcaagtgtgt atagccaaaa atcaggtaac
     8821 tccttgccgg agctgaaaaa atttgtatct gaaaacctga atcgtgtatt ggctgaggca
     8881 aaaaaacatc catcgattga agtacaagct gaaggtgtga ctaatcacgt taattataac
     8941 aacaaaggga atattgatgg ttgggatacc tctggtagca ttcagttgaa aagcaaagat
     9001 tttgatgcaa tggcagcggt gttggaaaat ttaggtaaag atgtggcgat tcgttacatt
     9061 gattttagtg tatcgccaga gaaaatggcg tcccttgaag atgaaatgac gctggaaatt
     9121 atcagcaaat ttcagcataa agccgatgtg attcaaaaag ccttaggggc gaaaacatat
     9181 aagctagtta atatcaatat tcaaactcca aatgatggtg gtagaccata ttatgaacac
     9241 agagcaatgt atgcagtttc atcaatggca caaagcaaat ctatggatga aatcccgtta
     9301 gaagcaggta aaaccacaat ttcagctacc gcttcaggaa gtgtggaatt tgaatagaaa
//
LOCUS       DANLXQ010000097         9312 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.097, whole genome shotgun sequence.
ACCESSION   DANLXQ010000097 DANLXQ010000000
VERSION     DANLXQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.097"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            49..264
                     /locus_tag="PXD94_001775"
     CDS             49..264
                     /locus_tag="PXD94_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDL6286618.1"
                     /translation="MFPEFRDLITQLKQNNSHFARLFDKHNELDQRIKNMEANIELAT
                     HEEVEALKKEKLHLKDQLYAILKQESA"
     gene            complement(323..1480)
                     /locus_tag="PXD94_001776"
     CDS             complement(323..1480)
                     /locus_tag="PXD94_001776"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL6286619.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(1602..3668)
                     /gene="prlC"
                     /locus_tag="PXD94_001777"
     CDS             complement(1602..3668)
                     /gene="prlC"
                     /locus_tag="PXD94_001777"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL6286620.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAEDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLSE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLAARSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRPYFPENRVLSGLFEVIKRIFGMRVEEQKDVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGIVGNAE
                     PVDCTQ"
     gene            complement(3799..4539)
                     /gene="pflA"
                     /locus_tag="PXD94_001778"
     CDS             complement(3799..4539)
                     /gene="pflA"
                     /locus_tag="PXD94_001778"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL6286621.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMSPPKEDLERIQKIIESYGHTVKF"
     gene            complement(4788..5813)
                     /locus_tag="PXD94_001779"
     CDS             complement(4788..5813)
                     /locus_tag="PXD94_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL6286622.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVHSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            complement(6048..8372)
                     /gene="pflB"
                     /locus_tag="PXD94_001780"
     CDS             complement(6048..8372)
                     /gene="pflB"
                     /locus_tag="PXD94_001780"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL6286623.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            complement(8462..9286)
                     /gene="focA"
                     /locus_tag="PXD94_001781"
     CDS             complement(8462..9286)
                     /gene="focA"
                     /locus_tag="PXD94_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL6286624.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     ASPEFWTAINVDPTQFADLDVSHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
BASE COUNT         2612 a         2105 c         1838 g         2757 t
ORIGIN      
        1 gctagtattt aatcaagtaa aatttgcaac cactattagg agttaaatat gtttccagaa
       61 tttcgtgatc tcataaccca attaaagcaa aacaattcac attttgctcg cttatttgat
      121 aaacataatg agctagacca gcgtattaag aatatggagg caaatattga actggcaaca
      181 catgaagaag ttgaggcact aaaaaaagaa aaattgcatt taaaagacca actttatgca
      241 attctaaaac aagaatcggc ttaacctaaa gtaaaagctc agaaaatatt ctgagcttta
      301 atttcggtca tattagacaa tattacagtt ttaccggctc aataatttcg cttgggtagc
      361 aacccaacac tttgaggtag ctggtaactt gctccaattc cgccaatgct ttttgggtgt
      421 tctcggaatg aatatttgcc tccagctcca cataaaacat ctcttcccaa ggtttgccgt
      481 aaatcgggcg ggattccagt ttcatcatac gaatatgatg atttttgaat accattaacg
      541 catcgaccaa tgcacccgcc tgttgcgtgg tggtcattaa cagtaacgtt ttggtttgta
      601 cctgtggcga aacctgcaca gcctctttgg caacaacaat aaaacgggtg atattgtgag
      661 cttgattagc aatatcggtt ttgatattcg tcaagccata caattttccg ccatcttcat
      721 tgcccaaggc aacaatattc ggcttattca agcgagccac catttgcatt gcgtgcgagc
      781 tgctttcgca atatttgata tgtactttat ctagcccggc taaaaattgg ctacattgct
      841 gggcaggctg agggtgggtg taaatggtat ccacctccgc caaatcaacc gtgccgtttg
      901 ccaatacaca atgtttgatt gggtacgcca attcacccac taaggttaaa tcggtatgct
      961 gcaataaatc atacacttca ttgatcgaac ctgaagtcgt gttttcaagc ggcagtacgc
     1021 caaaatcggc ttcaccaagg ctgactttct caaatacttc attaaaagaa tggcagctta
     1081 attccgccaa tgtgccttga tattttttgg caaactgtcg agatgccata ttagaataag
     1141 agcctcgcat tcccaaaaac gcaatcgaaa cctgctcttc tttttgtgca ttcaatttat
     1201 tttgcagata attttgctga gtcagcactg aatcttcaat aatacgttgg aaaatttggg
     1261 tcacatattg cggatcaagc tggtaattct ccgcttcggc aaaatttacc agttcttgaa
     1321 gcaaaatctt ttcccgttct aaatctctca agggtttttg tgtgatttct ttgctccgca
     1381 ccacatcaaa ggctaagcgg tggcgttctg ccagtagctt taataaattg cggtctaagt
     1441 tggttatttg ggttcgaata tcggctaaat ctaaagacat tatttcactc tctgtcaggc
     1501 taaatgctga aaattataac gagttgcaaa agaaagaaaa gacaagcggt caaatttaca
     1561 aaaaaatttg caaatctaac cgcttgtaaa acgtgtttac tttattgagt gcaatcaaca
     1621 ggctcggcat tacctacaat gcctttgtgt ctgagcaagg catcaagggt tggcttacgc
     1681 ccacggaagc gttcgaacag caccattggc tcttctgaac cgcctcgggt taagatatta
     1741 tctaagaaag actgccccgt ttcagggttg aaaatcccct cttcctcaaa gcgtgaatag
     1801 gcatctgctg aaagcacttc cgcccataaa taactgtagt agcccgccgc atagccgcct
     1861 gcaaaaatat gcgagaaact gtgtggcgtt ctcgcccact ctacgccttt ggcaacagcg
     1921 acttggtctt tcacctcttt aagtaaggct aacacttgtc cttgtttgct cgggtcatat
     1981 tcggtgtgta ggcggaaatc aaacaagcca aattctaatt ggcgaagcac aaacattgcc
     2041 gcttggaagt ttttcgccgc caataattgc tctaatttcg cttttggtaa cggctcgccg
     2101 gtttcataat gccctgaaat aaaggctaat gcctcttctt cccagcacca gttctctaag
     2161 aattggcttg gcagctccac tgcgtcccac ggcacaccgt taatgccggc gacatcgccc
     2221 acgtcaattt cagttagcat atggtgaatg ccgtgtccaa actcgtggaa caaagttgtc
     2281 acttcatcgt gggtaaacaa tgccggttta tcgccaatcg gtttattgaa gttacaggtt
     2341 aaatatgcca ccggtttttg taatgtgccg tcagccaagc gtttttggtt gatgcaatca
     2401 tccatccacg ccccaccacg tttgttttca cgagcgtata aatctaagta gaacgagcca
     2461 cgcaagcggt catttttatc aaaaatattg aaaaaacgta catctttgtg ccatacatcc
     2521 acatcttttt gttcttccac acgcataccg aaaatgcgtt taattacttc aaataagcct
     2581 gataatacgc gattttccgg gaaatacggg cgaagttctt catcattgac cgaatacaat
     2641 gcctgttttt gtttttcgct gtagaacgga atatcccata aatccaaatg ttcaatgccg
     2701 aaatgggctt tagcaaattt tttcaaatcg cataattcat ttttgccttg agctttcgag
     2761 cgagcggcta aatcgttcaa gaaatctaat acctgttgtg gattttctgc cattttggtg
     2821 gctaatgaat aatcagaata agtcttaaag ccgagaagtt gggctttttc gtgacgcagt
     2881 tttaagatct catcaatcac cgcactgtta tcccatttgc ctgcattcgg gccttgatca
     2941 gaagcacgag tcacataagc acggtacatt tcctcacgca gtgctttgtt ttcacaatac
     3001 gtcatcaccg gcaaatagct tgggatttca agagtgaagc gatagccttt cttacctttg
     3061 ctttctgcgg ataattttgc cgcttctaat gccgattccg gcaagccttt aagctgtgct
     3121 tcatcggtaa ttacaatatc ccaacccatt gttgcatcaa gtacgttgtt gctaaattgc
     3181 gagttgagtt ccgacaagcg tgcagaaatt tccccgtagc gtttttgttt ctctgccggc
     3241 agtgaaatgc ctgataattc aaaatcttgt aagctgtttt caatcgcctt tttctgtgca
     3301 accgaataag tttcaaactc agggctgttt tttaacttca catagccttg atataaccct
     3361 tgatgttgcc ccgcccaagt gctgtattcc gataataacg gcaagcacgc ttggtaagcc
     3421 tcacgcaact ctggggaatt tttcaccgca ttcaaatgcc ctaccggtga ccacgcacgg
     3481 gagaatttat cctctgccat tgcttgtggc atatagaaat tttcccacgt cgggttttca
     3541 atttgtgcga ctttttcaac agtttcacgg cattctttga tcaccgcttc taccgccggt
     3601 ttgatatgtt caggtttgat ttgtgagaac gcaggcaagc ctgtgtaatt taaaagaggg
     3661 ttagacatta ttttttcctt ttatggtcaa aatgaaaatt cgtatcaaga taatacacaa
     3721 agtgagagag aaaagaaaac aagcggtcat atttcaataa ttttttgcaa aaaaatcaca
     3781 aaatatgacc gcttgtcttt agaatttgac tgtatgcccg taactttcaa tgattttttg
     3841 gatacgctct aaatcctctt taggcggtga cattacccct tccagctcat atttatagcc
     3901 taaggtttcc catttgtgag cccctaaacg atgataaggc agaagttcca ctttctcaat
     3961 gttatccatt ccttgaataa actgccctaa acgatgggca gaatcatcgt catcagtata
     4021 acccggcacg accacatagc gaacccaagt cggtttattt aatttgtgca gataacgggc
     4081 gaaatcaagg gtacgcttat tcgacacccc gactaaatct tggtgaactt catcattgag
     4141 ctgttttaaa tccagcaaga ccaaatcggt cacttcaagc atttcatcca ccaccgggct
     4201 ataatggcga acaaaaccat tggtatctaa gcaggtatta atgccttcag ccttacaggc
     4261 acgaaaccaa tcccgcacaa actccatttg caacaccgct tccccacccg aagctgttac
     4321 accaccgccg gttgctttca taaaatgctt ataagtcgtg acttctttca tcagctcttc
     4381 aaccgtaatt tccttaccac catcaagatc ccaagtatca cgattatggc aatatttaca
     4441 ccgcatcaaa cagccttgca agaacagaat aaaacgaatc cccggtccat ctaccgtacc
     4501 gcaggattca aaagaatgga tacgtccaac aacagacatt tgtcacctca accctcaaaa
     4561 aattttgaga aattgtagca aatttttatg ttgttcgcag aaataaatca caccggtata
     4621 ttttaagaaa ggagaaagtc atactatttt ttgcctattt caggaataaa cttttattaa
     4681 tctattgact ccatcaacat atcaaccaaa tatgtttaaa tttttgcatt ttttaaagct
     4741 attttgaccg cttacaagca gtcggttttt ctgaaaactt tgcaaaccta gccggcgtgg
     4801 tatttggcgg cgacctcttt taccagtaag tcgaaatcgc tttcgtaggt gagatcctgc
     4861 ttgactcggt atttttcaaa ttcgctgagg gcgaaagatt tagcaatttc agcggtaact
     4921 ttgccggcat cttgtaagat ttctcggtcg gtggcggcaa gaaagcggtt aaggcgggtt
     4981 tcccagtctg ccattgtcat cggaatttgg cgttctgcca tatcttccgc catatccaaa
     5041 taggcagaga ccagccgttg tagttgtgct aattcattat ccgataaata atttttggca
     5101 accaccacat caaaggggta aattttgcct tgcggagcgt ctttccaatt cgttagcccc
     5161 atattcggct tttcagcgtt ggctcgtgcc ataatcagct cggcggcggt atgcccgtga
     5221 attgcccaat ggagcttgtt ctgcacggtg gcaaaaaagc gtttggtggc ggttgcggtg
     5281 cggtcgtagt ccacggcggt agcgtaaatg tcggtgattt tttggtagaa cttgcgttcg
     5341 gataaccgaa tttcccgaat acgagcaagc tgttcttcaa agtatttttt gcccaaaatc
     5401 gtgccgtcat ttttcagccg ttcatcgtcc atcgcaaagc ctttaatcgc aaagctatgc
     5461 acgatttcgg ttgcccattt gcgaaactgc accgctcgct cggaattgac tttataaccc
     5521 accgcaataa ttgccgacag attataatgc ttggtgtggt agtttttgcc gtcttgggca
     5581 gttatccaaa aaaattggat aactgaattt tcagctaact cattgtcact aaagattttt
     5641 ttgagatgat agctaatggt tcgtacatca acatcataca acaccgccat cattttttgg
     5701 ctaagccata cattttcatc ggcgtaaacg gcatcaactt ggctttcgcc ggtagctgta
     5761 ataaaagtga ggtattccgc cacagaggaa cgaattaaat cttgcttaga catctctttc
     5821 tcccatatag aacactgtaa ttataaacaa tattacgaaa gctgtgtaaa tgaatagcca
     5881 atttttcgag cgagatcaca aaaaaaccgc ttgcggcata aatgctacaa gcggttgatt
     5941 attactcaca aaattgtttc caattttgtt cgctcctacg gagccgtcgg caaagccaac
     6001 gttcaaaacg caatgcgttt tgtggctaaa agttttgcaa attgtaatta catactttca
     6061 gtgaaggtgc gggtaattac gtccatttgt tgctctttgg ttaaagagtt gaaacgtacc
     6121 gcatagcctg aaacacgaat cgttaattgc ggatattttt cagggtgttc taccgcatct
     6181 aataatgtat cacggtttaa cacgttcaca tttaagtgct gaccgccttc tacagttgct
     6241 tcgtggtgga agtagccgtc cattaagcct gcaaggttac gtttttgggc ttcgtaatct
     6301 ttacctaacg catttggtac gatagagaaa gtataagaaa taccatcttt cgcataggca
     6361 aacggtaatt tcgccacaga agttaatgaa gctaccgcac ctttctggtc acgaccgtgc
     6421 attgggttcg cacccggtcc gaatggagca cctgagcgac gaccgtccgg tgtattaccg
     6481 gttttcttac catataccac gttagaagtg atagtaagca cagactgagt cggcgttgca
     6541 ttgcggtatg ttttgagttt ttgaattttc ttcataaaac gttcaactaa atcaactgca
     6601 atgtcgtcca cacggttatc gttgttaccg aattgcggat attcaccctc aatttcaaag
     6661 tcggttgcta cgtttttcgc tacaccaacc acttcgccag ctttgttttt gatttcaata
     6721 tcggtacgga ttggttttac tttcgcatat ttaatggctg aaagtgagtc tgccgctact
     6781 gaaagccctg cgataccaca cgccattgtg cggaacacat cacggtcgtg aagagccatt
     6841 aatgccgctt cgtatgcata tttatcgtgc ataaagtgga taatgttcag tgctgtcaca
     6901 tattgggttg caagccaatc cataaagctg tctaagcggg tcattacatc atcgtaatct
     6961 aagtattcac ttgtaattgc ttcagtttta ggccctactt ggtcgcctga tttctcatca
     7021 acaccgccat tgattgcgta taacaaggtt ttcgctaagt ttgcacgagc gccgaagaac
     7081 tgcatcattt tacccacgat cattggcgat acgcagcacg caatcgcata gtcatcgttt
     7141 tggaaatccg gacgcattaa gtcatcgttt tcatattgaa ctgatgaagt atcaatagac
     7201 actttcgcac agtagcgttt aaatgcttcc ggtaattgtt cagaccaaag aatagttaag
     7261 tttggctccg gtgacggccc cattgtgtaa agggtgtgta aaatacggaa gctgtttttg
     7321 gttactaagg tacgaccgtc taaacccata ccggctaagg tttcggttgc ccacattggg
     7381 tcgcctgaga ataattgatc gtattccgga gtacgcaaga aacgcaccat acgcaatttc
     7441 attactaaat ggtcgattaa ctcttgggct tcttgctcgg taattttgcc ggcttttaag
     7501 tcacgttcga tataaatatc taagaatgaa gacacacgac cgaaagacat cgccgcaccg
     7561 ttttgcgatt taaccgctgc aaggtaagcg aaatatgtcc attgcaccgc ttcttgtgca
     7621 ttggttgcag gacctgaaat atcaaaaccg tagcttgcag ccatctcttt cattttgcct
     7681 aaagcacggt gttgctcggc aatttcttca cgcagttgaa tcgtggcttg aatatcttcg
     7741 ccggattcta aacgagcttg taatgaggca aactggcgtt gtttgtcttt cattaagaag
     7801 tctgcaccat aaagtgccat acggcggtag tcaccgataa tacgaccacg accataagca
     7861 tccggcaaac cggtaattac acctgattta cggcaacgta aaatatccgg agtgtaaaca
     7921 tcgaataccc cttggttatg ggttttacgg tattcggtaa agatttgttc tacttcaggt
     7981 ttaagctcac ggcgataaac tttgcacgaa cctttcacca tattgatacc accaaacggc
     8041 ataatcgcac gttttaatgg agcatctgtt tgcagaccaa cgattttttc taacgattta
     8101 tcaatataac ccggtgcgtg tgaggtaatg gttgatggcg tgtcgcagtc aatatcatac
     8161 ggttcgtgtg ttttgttttc tactttgatc atttccatca catctgccca caatttgctg
     8221 gttgcaggtg ttacttcagc taggaaagac tcatcaccct cataaggtgt gtagtttttt
     8281 tggataaaat cacgtacatt tacttcagtt tgccactcgc ctgcggcaaa accttcccac
     8341 gctttttgtt gagcgtctgt tagttgagtc attttaagtc ctctgtgtta gtaagttaaa
     8401 acgggaaagc ggtgattttt tgcaaaaaat ttgcaaaatt tgaccgcttg aatctgaaat
     8461 cttaatgctt tctaatgtat aagaaccact gcattaaacc aatgaaaaac acgccaccga
     8521 caatattacc cagcgttgcc ggaattaagt tcttaaacac aaaatgagat acatctaaat
     8581 cggcaaattg cgttgggtca acattaattg ctgtccaaaa ttcagggctg gcaaagtgcg
     8641 aaatcgcaat ccccatcgga atcataaaca tatttgccac acagtgttca aagccggaag
     8701 ccacaaacat tgcaatcggt aacatcataa taaaggcttt atcggttagg cttttacctg
     8761 cataagacat ccacaccgca atgcacacca taatgttaca gaaaatgcct aaacagaaag
     8821 cctcaaacca ttggtggtga attttgtgct gtgcggtatt taaaataacc aaaccccatt
     8881 gccctttggc tgccataatc tgaccactaa accaaatcaa caatacaatg gaaatcgccc
     8941 caacaaagtt accgaaatac accactaccc agtttttaaa catctgtaac cagttaattc
     9001 tttggctggc tctggctaca atcgtaagtg ttgatgaggt aaaaagttct gccccaaaca
     9061 ccacacacat aatcacgccg agtgaaaata ccaaaccacc aatcagttta gccatacccc
     9121 aaggcatttg gctcgcaccc acttgggttg tggtataaaa tacaaatgct attgcaataa
     9181 aaccgcccgc agtaatggca gagagaaagg aatataattg attttttgtt gctttataaa
     9241 ccgcaccatc ttctgcgatt tttgccattt cagcaggaga aaacatcaga caaccttaac
     9301 aatgagaatt ga
//
LOCUS       DANLXQ010000098         9305 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.098, whole genome shotgun sequence.
ACCESSION   DANLXQ010000098 DANLXQ010000000
VERSION     DANLXQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9305
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.098"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(26..1471)
                     /locus_tag="PXD94_001782"
     CDS             complement(26..1471)
                     /locus_tag="PXD94_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286625.1"
                     /translation="MRSTFKLTLAALAVSIVLSGCKSSQGPSIVADSPPPKEITATDG
                     NDKEKTDNKEKKSAQPNKSDPQPDKPANAKDPVKPDAETNKPSKKDKEYTPPATEKID
                     NSSVQPDKMVPNPIENKENDSNKPNPKESDSDNNDKVTEGNKPNAPTETVNKDKEAEK
                     SKKENKEDSSNNTPNINFDGAKPIDTDSKGSPYRVINLNPRKSVVNASAMQKVDFDSF
                     TDESVENSPFVSSDTEKEYNNTLVFGLGAGDKDSYFKNLNQKGDKHLGIHSGVYEDAN
                     LEGNTKKINYLYVNQPYSSYGALFTDQKDSHLFNVRLTAGRENGDSYAEYGVFTQSGK
                     EAKWNDNLIGDATYKGQAIARVIRQVNGEQVAGIPQFDGDVTLNLHLSKEWNESKLNG
                     KIDSQSVGVITLKESEIANPTALRSNIGFGEEVIVENKENLVGDYAVDFTGPQLEEAV
                     GVVELENEAETFSENEVIKYNAVFGVSKAAK"
     gene            1656..4004
                     /locus_tag="PXD94_001783"
     CDS             1656..4004
                     /locus_tag="PXD94_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6286626.1"
                     /translation="MLISAQFSPLVWAENNDVAVLDEVSVVGSTPSISQGSEVTLLKV
                     SDKIIAGKEFKKRSATLGNALASELGVHSNPFGGGASKPIIRGQEGARIRILQNGSDV
                     IDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVGIVNVVDKRIPTAIPEKGY
                     EVELNSRFDTAAKEKVGALGATFGIGNHIAVRVEGLSRHSDNYRVPGINLGERLNYVP
                     DTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNHKFDFCTGHIYGNKRDKYA
                     YTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGHAHDHTHKGPWVDLESKRI
                     DVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYHKQLKKDQDYGKPVNIFKN
                     RGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREVKFPLVENTNKQFSLFGVE
                     QYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISGGKQVEPDLSPYDETAYAY
                     SGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATNSFEYGNKDLKKEQSNNVE
                     LGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRRYNQAKARFYGVEAEASYR
                     FNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPENVEKGKDYLLYRADQNTPR
                     TPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYITEGNTMLNVGLSYSNKWKE
                     LDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEMKF"
     gene            4361..6631
                     /gene="nrdA"
                     /locus_tag="PXD94_001784"
     CDS             4361..6631
                     /gene="nrdA"
                     /locus_tag="PXD94_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL6286627.1"
                     /translation="MNKSLMVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELR
                     SHIQFYEGIRTSDIHETIIKAAADLITKDTPDYQYLAARLAIFHLRKKAYGQFEPPHL
                     YEHVKKLVALGKYDADLLSDYTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNR
                     VSGEIYESAQFLYLLVAACLFSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTP
                     TRQFSSCVLIECDDSLDSINATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMD
                     YGVQINKMMYQRLIKGGEISLFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRK
                     VKAVELFSLLMQERASTGRIYIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLA
                     HFYDEQGEIALCTLSAFNLGTLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSL
                     NRRALGVGVINYAYYLAKNQVRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACA
                     YFDQTTYAKGLLPIDTYKKEIDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSET
                     SSQISNATNGIEPPRGYISVKASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLV
                     GIMQKFVDQAISANTNYDPNRFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAED
                     VQDDLDEGCAGGACKI"
     gene            6658..7788
                     /gene="nrdB"
                     /locus_tag="PXD94_001785"
     CDS             6658..7788
                     /gene="nrdB"
                     /locus_tag="PXD94_001785"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL6286628.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMAYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(7869..8639)
                     /locus_tag="PXD94_001786"
     CDS             complement(7869..8639)
                     /locus_tag="PXD94_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL6286629.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIEKNADAILVHHGYFWKSENPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHSELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(8723..>9305)
                     /locus_tag="PXD94_001787"
     CDS             complement(8723..>9305)
                     /locus_tag="PXD94_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL6286630.1"
                     /translation="YTTIMQRISGAKQIDSLTVKIKDDIESQTAEKSIAELLVAKHGK
                     KDFFIMNSDTIKQTITSTTNTMTLLISSIALISLIVGGIGVMNIMLVSVTERTREIGV
                     RMAIGAKQRNILQQFLIEAVVICLIGGVIGILLASAIIWAFNTLGTNFKMMLSPESVI
                     IAVLCSTLIGVVFGYIPARNAAQLNPITALAQE"
BASE COUNT         2688 a         1766 c         1962 g         2889 t
ORIGIN      
        1 agccctttgt aagatacaaa aaatactatt tcgccgcttt gcttacgcca aataccgcat
       61 tgtatttaat gacttcattt tcagagaatg tctcggcttc gttttctaat tcaacaacgc
      121 ctaccgcctc ttcaagctga ggcccggtaa aatcgacagc gtaatcaccc acaagattct
      181 ctttattctc aactatgact tcttctccaa aaccaatatt tgagcggagt gcggtagggt
      241 tagctatttc actttcttta agtgtaatta ccccaacact ttggctatca attttgccgt
      301 ttagtttact ttcattccat tctttggaaa ggtgtaaatt taatgtaaca tcaccatcaa
      361 attgaggaat acctgcgact tgttccccat ttacctgacg aataacgcga gcaatagctt
      421 gacctttgta agtggcatca ccaatcaggt tatcattcca tttagcttct tttcctgatt
      481 gtgtgaatac accatattcg gcataactat ctccattttc tctaccagcg gtcagacgaa
      541 cgttgaataa atggctgtct ttttggtccg taaataaagc accatagctc gaataaggtt
      601 gatttacata gagatagtta atttttttag tattaccttc taaattcgca tcttcataca
      661 cgccactatg aatacctaaa tgtttatccc ctttttgatt taagttctta aaataagaat
      721 ctttatctcc ggcaccaagc ccaaaaacaa gtgtattatt gtattctttt tcagtatcgc
      781 tactgacaaa tggtgaattt tcaacagatt catccgtaaa agagtcgaaa tctacttttt
      841 gcatagcact tgcatttact acgctttttc tcggatttag gtttattact cggtacgggc
      901 tacctttact atctgtatct ataggctttg ccccgtcaaa attaatgtta ggagtattat
      961 tgcttgagtc ctctttattc tcctttttac ttttctcagc ttctttatct ttatttactg
     1021 tttctgttgg ggcatttggt ttattgcctt cagtgacttt gtcattatta tcgctatcgc
     1081 tttctttggg gttaggttta ttactgtcgt tttccttatt ctcgatagga ttaggcacca
     1141 ttttatccgg ctgtactgat gagttatcga ttttttcagt agcaggtggt gtatattctt
     1201 tatctttttt acttggttta tttgtctccg catcaggttt tactgggtct tttgcgttag
     1261 caggtttatc cggttgagga tcggatttat ttggttgcgc agactttttc tctttgttat
     1321 cggttttctc tttatcgttg ccatcagtag ctgttatttc cttaggtgga gggctgtctg
     1381 caacaataga tggaccttgg ctagatttac agccacttaa aacaatagaa acagctaaag
     1441 ctgcaagagt aagtttgaat gtagaacgca tattttgtct ccgtcagatt aaatccataa
     1501 aattttaatg gatatatttc ctgtttggca atagggaaaa tttaaaaaat ataatttttt
     1561 tatggttatt attgtaaaga ctcatatttt gcagtacctt attatcctta atttttttaa
     1621 ggagaaagat tgtgtttaag aaaacaggat tggcattgct gatttcggct caatttagcc
     1681 cgcttgtttg ggcggaaaat aacgatgtag cggtgttaga tgaagtttcg gtcgttggca
     1741 gcactccaag tatatcgcag gggagtgaag ttaccttgct aaaagtcagt gataaaatta
     1801 ttgcaggtaa ggaatttaaa aagcgttcgg caacgctggg taatgcgttg gcgtcagaat
     1861 taggcgtgca tagtaacccg tttggtggcg gagcgagtaa gccgattatt cgtgggcagg
     1921 aaggggcgag aattcgcatt ttgcaaaacg gttcagacgt gattgatatg tcgaacctat
     1981 ctcctgatca cgccgttgtt gctgattcac tgcttgccga tcaggtcgaa atcttgcgtg
     2041 gttccagcac cttgctttat gccagcagtt cgccggtagg aattgtaaat gtggtggata
     2101 aacgtattcc gaccgctatt cctgaaaagg gatatgaggt tgaactcaat agccgttttg
     2161 ataccgccgc caaagagaaa gtcggcgcat taggggcaac ctttggcatc ggtaaccaca
     2221 ttgcggtgcg agtcgaaggg ctgagtcgtc atagtgataa ctaccgtgtg ccggggatca
     2281 acttaggcga gcgcttaaac tatgtgccgg acacttacaa taaatccaaa gtcggcacgt
     2341 tgggcttatc ctttgtcggt gaacgaggct atattggtgc ctcatacagc aaacggcggg
     2401 ataattatgg cttgccgggg cataaccata aatttgattt ctgtaccggt catatctacg
     2461 gtaataagcg ggataaatac gcttatacct atctctatcc gcatttgatt ggtgaggaaa
     2521 atattgggag caatccgcac ttccactgtg gaaccaacca cgccgaggac ggcacacaca
     2581 gccacgataa cccgttcgga cacgcccacg atcacactca caaaggcccg tgggtggatt
     2641 tagaatcaaa acggattgat gtgaaagccg agctacgcca gccgtttaag ggcgtagata
     2701 aaattaaggc aagctatgct gatgccgatt attaccacga tgaaaaagat gcaggcgtgc
     2761 ttgccacccg ttaccataaa cagctgaaaa aagatcagga ttacggcaag ccggtgaata
     2821 ttttcaaaaa tcggggcaaa aatacccgct tggaggtcta tcacgcaccg ctaggtggct
     2881 taaccggtgt atggggtgtt cagtatcaaa cgcaaaaaag cagtatgaat gcgccgaaag
     2941 atcgcgaagt gaaattcccg ctggttgaaa ataccaataa acagttcagc ctgtttgggg
     3001 ttgagcagta tatgtgggat agcgttgctg ttgagcttgc cggacgaatg gaaaagcaga
     3061 agattgagat tgagtatgat cgcaatgaaa tcaaacggtt acaagaacac taccgtatca
     3121 gtggcggcaa acaggttgag ccagatcttt caccctatga tgaaacggct tatgcctatt
     3181 ccggcacgct taactggttc ttccaccctg actatcagct ttcgtttact gcctcgcaca
     3241 atgaacgctt gccaacgcca atggagctgt attatcacgg gcagcatatt gctactaatt
     3301 cgtttgaata cggcaataaa gacttgaaaa aagagcagtc aaataatgtg gagttgggct
     3361 tgggctatca agctgaacgt attggctata aagtgagtgt ttattacaat cactttaaaa
     3421 actacatcta caatgaaaat cttttccgtg aaaatcagct atttatgcgc cgttataatc
     3481 aggcaaaagc ccgtttttac ggggtagaag cagaggcaag ctaccgcttt aacgaccaat
     3541 accaagccac gattttcggc gatatggtac gaggctggct aacaaattta ccaccattga
     3601 agataaatag cgattatagt gtgttcaaag attatctgcc ggaaaatgtg gaaaaaggga
     3661 aagattatct tctatatcgg gctgaccaaa acacccctcg tacaccgcca atgcgtttag
     3721 gttttcgctt taatgcggaa tttaccccaa attggtcggg ggatttagag ctgatccgca
     3781 cctttaccca acgccgtacg agccagttgg aatatattac cgaaggtaat acgatgttga
     3841 atgtcgggtt gtcgtatagc aataagtgga aagagctgga ttacaaaatc agtctaaacg
     3901 gcacaaactt actgaatcag ccggtgtata tccacacttc gtatcaccaa tttgtaccac
     3961 aaatgggacg aaattttatg ttgggaatgg agatgaaatt ttaagttctc gacataacaa
     4021 gacaagcggt tattttttct tcaaattttg caaaaaaacg gctaaaaata accgcttgtt
     4081 taaaactcag gtattggtaa tttggataaa atagtgctgt ttttcctttc ttgttatttt
     4141 cggagcttac caaaatttcc tgtttgttgt taaataagac aattaacttt atccctttat
     4201 taaaaatatg gttatttata aataacttag catctgtcaa gagaaaaagt aaaaatttat
     4261 tggttgatgt tttgctttaa gcacactatc ttgtgtttac tttaatgaaa aacacaagat
     4321 gtagtgtttt actaaggtat tttataaagg gttaataatt atgaataaaa gtttaatggt
     4381 aacaaagcgt aatggcacgc gcgagcaact caatctagat aaaattcatc gtgtgattac
     4441 ttgggcggca gaagggttag agaatgtttc tgtttcacaa gtagaactcc gttctcacat
     4501 tcaattttat gaaggtattc gcacttcaga tattcatgaa actattatca aggctgcagc
     4561 tgatctaatc actaaggata cgccagatta tcaatattta gccgcacgct tggcgatttt
     4621 ccacttacgc aaaaaagctt atggacaatt tgagccaccg catttatatg agcacgttaa
     4681 aaaattagtt gcgctaggta aatatgatgc agacttgctg agcgactata cgcccgaaga
     4741 atgggagaca atgaatggat ttattgatca ctggcgggat atgacgtttt cctatgcagc
     4801 agtaaagcag ttggaaggaa aatatttagt gcagaatcgt gttagcggag agatttatga
     4861 atcggctcaa tttttgtatt tactcgtagc tgcctgttta ttttctaagt atccaacagc
     4921 aagccgctta gattatattc gccgttttta tgatgcgact tccactttca aaatttcttt
     4981 gccaacaccg attatggcag gagtgcgtac acctactcgt caatttagtt cctgtgtgtt
     5041 aattgagtgt gatgatagct tagattccat caatgcaacc tcggctgcaa tcgttaaata
     5101 tgtttcacag cgtgctggga tcggtattaa tgccggcgca attcgggcat taggtagccc
     5161 cattcgtggt ggagaagcat ttcataccgg ctgtattccc ttttataaac attttcaaac
     5221 tgcagtaaag tcttgctctc aaggtggtgt tcgcggtgga gcggctaccg tgtattttcc
     5281 gatttggcat ttggaagcag aaagcttaat ggtattaaaa aataaccgtg gtgtggaaga
     5341 aaaccgtgct cgccatatgg attacggggt acagattaat aaaatgatgt atcaacgttt
     5401 aattaaaggc ggtgagatta gtttatttag tccatcagat gtgccggggt tatatgaagc
     5461 gttttttgct gatcaagcta aatttgagca actttatttg caatatgagc aagatccgag
     5521 cattcggcaa cgtaaagtta aagctgtaga attgttttca ttactgatgc aagagcgggc
     5581 ttcaacaggg cgaatttata ttcagaatgt cgatcattgt aatactcata gcccatttaa
     5641 tcctcgtgtc gcaccgatac gccaatcgaa cctctgctta gaaattgctt tgccaactaa
     5701 gccactggca catttttatg atgaacaagg ggaaatcgca ttatgtacat tatcggcatt
     5761 taatttaggc acactcagca atttagatga gttagaagaa ttggctgatt tagtggttcg
     5821 tgcgctagat gccctattag attatcaaga ttatccggta ttagctgcac gtaacagctc
     5881 attaaatcgt cgcgcattag gtgtaggcgt gattaactat gcttactatt tagctaaaaa
     5941 ccaagtgcgt tattccgatg gcagtgctaa taatttaact caccgtacct ttgaagcgat
     6001 tcagtattat ttattaaaag catcgatgaa tttagccaaa gaacaagggg cgtgtgctta
     6061 ttttgatcaa accacttatg ccaaagggtt actccctatc gatacttata aaaaagaaat
     6121 tgatgaatta actcaagaac cgcttcatta tgattgggaa caactacgtc aggatattct
     6181 gcaatttggc ttgcgcaatt ccactttaac tgccttaatg ccatcggaga cttcgtcgca
     6241 aatttcgaat gccaccaatg gaattgaacc tccgcgtggc tatattagtg ttaaagcatc
     6301 aaaagacggt attttaaagc aggttgtgcc ggagtatgaa cgtttaaaag catactatga
     6361 actgctttgg gatattccta atatggacgg atatttgcat ttggttggga ttatgcagaa
     6421 atttgttgac caagcgattt cggcgaatac aaattacgac ccaaaccgct ttgcagaagc
     6481 taaagtaccg atgaaagtat tattaaaaga tctattaacg gcgtataaat acgggttaaa
     6541 aaccctttac tatcaaaata cccgtgatgg agcagaagat gtgcaagacg acttagatga
     6601 aggctgtgct ggtggtgctt gtaaaattta ataagaacag aaataaagga gaataacatg
     6661 gcatatacta cgttttcaca acataaaaat gatcaactaa aagaacccat gttttttgga
     6721 caaaatgtga atgtggcgcg ttatgatcaa caaaaatacg aaatttttga aaaactgatt
     6781 gaaaaacaac tctcattttt ctggcgaccg gaagaagtgg atgtgtcaca agaccgcatt
     6841 gactatgccc aactgcctga acatgaaaag cacattttta tcagcaattt aaaataccaa
     6901 accttgttgg attccattca agggcgtagc ccgaatgtgg cgcttttgcc attagtgtct
     6961 atccccgagc ttgaaacctg gattgaaact tggacattta gcgaaacaat tcacagtcgt
     7021 tcttacacac atatcatccg taatattgtc aatgatccat caatagtatt tgatgatatt
     7081 gtcacaaacg aagaaattat taagcgtgcc aaagatattt ccgcctatta tgatgatttg
     7141 attcgtgaca gccaactcta tagtttgtat ggtgaaggca gttatcaggt tgatggaaaa
     7201 acctgtgagg tcagtttgcg tagtctaaaa agacaacttt atctctgtat gatgagtgtg
     7261 aatgcgcttg aagcaattcg tttttatgtc tcttttgctt gctcatttgc ctttgcggaa
     7321 cggcagctta tggaaggaaa tgccaaaatc atcaaattta ttgcacgtga tgaagcatta
     7381 catcttaccg gcactcagca tatattgaat attatggcct atggcaatga tgatcctgaa
     7441 atggcagaaa ttgcacgtga atgtcgccaa gaggcctacg agcttttttt aagtgcagct
     7501 gaacaagaaa aagcgtgggc ggaatacctg tttaaagacg gttcgatgat agggctaaat
     7561 cgggatattt tggtgcaata tattgaatat atcactaata ttcggatgca ggctgttgga
     7621 ctaccattac catttaaggc acgttccaac cccattccgt ggatcaatgc gtggttggtt
     7681 tccgataatg tacaggtagc accacaagaa gtggaagtca gttcttattt ggttggccag
     7741 attgatgcta aagtagatag ccgtgatttt ggcgattttg aactttagac tatgatatac
     7801 aagcggttga tttttagtga ttttttgcaa attgcaaaat tttcaggaaa ttcaaccgct
     7861 tgtatttttt atgcagggtt atcaatatca ataaattcaa catctaaacc atattcaacg
     7921 gctaaccatt cacctaaggc ttttacgcca tctcgttccg tggcgtgatg accgcaagca
     7981 aaataataga ttccttgttc acgggcagag tgagtggtct gttcggaaat ttcgccacta
     8041 ataaaggcat cacagccttt ttcaaaggct aaatcaatat agccttgtcc tccgccggag
     8101 caaatcccta ttgttttaat cattttttgt gggctatgaa caaattcatc acataaaata
     8161 acggtgcgtt gtaacgtttt ttcaagtttt tgtttcagtt cattggcgga aatcggattt
     8221 tctaattcac cgtacattgg aattgaatta ggtctgtctt ctaaaccttg tagatttttt
     8281 actcctaaat gcttagcaag ctgggcgttg ttacctagtt cggagtgaat atccagtggt
     8341 aggtgataac caaagagatt gatgtcattc actaaaagct gtttaatgcg tttacctttc
     8401 attcctcgaa tgcacgggtt ttcacttttc caaaaataac cgtggtggac taaaattgca
     8461 tcggcgtttt tctcaatcgc tttttcaatc aggggaagtg tagctgtcac accggtaata
     8521 atttttttga tctctctttt cccttcaact tgcaagccat ttggggcata gtcgttaatg
     8581 gcggagctgt tgagtttggt gttcagaatt tgttctagtt cagtattggt tattgtcatt
     8641 ttatctcctt aaacttggca taataatgga tgtaggggca aaaaattttt tgcccctaca
     8701 gtgtagcaga tagttatcgc atttactctt gtgccagagc agtaattggg tttaattggg
     8761 cggcgtttct tgccggaatg tagccaaata ccacaccgat aagtgtagag cagagcacgg
     8821 caataatcac cgattccggt gaaagcatca ttttaaaatt cgtacctaag gtattaaatg
     8881 cccaaataat agcgcttgcc agtaaaatgc caatcacacc acctattagg caaataacca
     8941 ccgcctcaat taaaaattgt tgcaaaatat tgcgttgttt tgcgccaatc gccattcgga
     9001 caccaatttc tcgggtgcgt tcggtgacgg aaacgagcat aatattcatt accccgatac
     9061 caccgacaat cagcgaaata agggcgatag aggagatcag caaagtcatt gtattggtgg
     9121 tactggtaat ggtttgtttg atcgtatcac tattcataat aaagaaatct tttttgccgt
     9181 gtttagctac taacaattcg gcaatgcttt tttcggcagt ttggctttca atatcatctt
     9241 taattttaac cgtgagtgaa tctatctgtt ttgcacctga aatgcgttgc ataatagtgg
     9301 tataa
//
LOCUS       DANLXQ010000099         9111 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.099, whole genome shotgun sequence.
ACCESSION   DANLXQ010000099 DANLXQ010000000
VERSION     DANLXQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.099"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(100..175)
                     /locus_tag="PXD94_001788"
     tRNA            complement(100..175)
                     /locus_tag="PXD94_001788"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Asn,seq:gtt)
     gene            complement(181..256)
                     /locus_tag="PXD94_001789"
     tRNA            complement(181..256)
                     /locus_tag="PXD94_001789"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Phe,seq:gaa)
     gene            complement(388..1479)
                     /gene="mltC"
                     /locus_tag="PXD94_001790"
     CDS             complement(388..1479)
                     /gene="mltC"
                     /locus_tag="PXD94_001790"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL6286631.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESALNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSSDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
     gene            complement(1496..1765)
                     /locus_tag="PXD94_001791"
     CDS             complement(1496..1765)
                     /locus_tag="PXD94_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL6286632.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            complement(1769..2914)
                     /gene="mutY"
                     /locus_tag="PXD94_001792"
     CDS             complement(1769..2914)
                     /gene="mutY"
                     /locus_tag="PXD94_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL6286633.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTLPERQTYFLVLKSGSKVLLQKREAKGIWGGLFVFPQF
                     ESLEALKRSDFMQNLQISQQLTAFRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEK
                     AGNYLSKVSSNADYWYDLHQHNEVGLATPVKRILDELAFSVKNEIKD"
     gene            2998..3390
                     /locus_tag="PXD94_001793"
     CDS             2998..3390
                     /locus_tag="PXD94_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL6286634.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            3391..4260
                     /locus_tag="PXD94_001794"
     CDS             3391..4260
                     /locus_tag="PXD94_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL6286635.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQHIKVWLISFLTFPLYVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     GYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKIMLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            4276..4989
                     /gene="sfsA"
                     /locus_tag="PXD94_001795"
     CDS             4276..4989
                     /gene="sfsA"
                     /locus_tag="PXD94_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL6286636.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIAATLTKKCEF"
     gene            5050..5571
                     /gene="hslV"
                     /locus_tag="PXD94_001796"
     CDS             5050..5571
                     /gene="hslV"
                     /locus_tag="PXD94_001796"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL6286637.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNYNHVIEEV"
     gene            5828..7153
                     /gene="hslU"
                     /locus_tag="PXD94_001797"
     CDS             5828..7153
                     /gene="hslU"
                     /locus_tag="PXD94_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL6286638.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            7229..8107
                     /locus_tag="PXD94_001798"
     CDS             7229..8107
                     /locus_tag="PXD94_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL6286639.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEGSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(8177..8893)
                     /locus_tag="PXD94_001799"
     CDS             complement(8177..8893)
                     /locus_tag="PXD94_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL6286640.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTEEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGITEPRKKQVLFSEPY
                     FPSSAAFIAKKDTDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
BASE COUNT         2705 a         1925 c         1835 g         2646 t
ORIGIN      
        1 ttttgaacgc ataatactaa aaattgagca atttcttagt agttagccgg aaagctcaaa
       61 aagaaaaccc tgatgattac tcatcagggc ttcagaatat ggctcctcct gcgggactcg
      121 aacctgcgac atatggatta acagtccacc gttctaccga ctgaactaag gaggaattga
      181 tggtgcctcg aggcggaatc gaaccaccga cacggggatt ttcaatcccc tgctctaccg
      241 actgagctat cgaggcaacg tcgttgcgtg gtgcattatg ctgatttcag cttgcaacgt
      301 caaacgtttt tttgcaaatt taaaaaaacc gctgctcaaa tgtgcttttt ttatactttt
      361 cggctaaaaa acagcataat tcacacatta acgaatattg cgatatttct ctctggcggt
      421 ggttactttt cttaaataat ttctagcttg agaagatgga tgagcagtgg ttaaaattcg
      481 ataaaccgca tcagacgaaa gatcgttaat tctatcaatc gcagagtatc ggtctgaatc
      541 aaacactcgt aataccgcac ctgctccgct attgtatgca gaaatcattg catagcgttt
      601 agccacagga tcttcaattc catctaagta ttcatcacgc aaaatagtca aataaagcgt
      661 acctgcatca atgttatttg atggattata aagatattca cgacttggct gccctcccat
      721 acctttacgg gcaaaaatat cacgcccggc ggtacgaggt acaacctgca ttaagccaat
      781 cgcattggca tagctgatgg cataagggtt gagtgctgat tcgacttcca taatccctaa
      841 aatcaaactt ggctcaatac catactgctt cgccatttta cgcaccagcg gtaaatattg
      901 tctggcacgt acttcaatgt gtcctgccac cattttaatc gttacaaagc tcacagtttg
      961 accatttttc aaacggcgtg tttgcacctt attttgcaac aaataagtcg caaaatcatt
     1021 ggcgatggca acattggtaa tatttcgccc gaattgatct tcaacctgtc cgtttaggaa
     1081 agggttagag ctaatcggtg catcgcctga ggcaaataaa tcaatccctt tcgggtctct
     1141 gcccattaat aaagtatgaa taatcgaatt gcgtagatga ttctgattcc ctaaagtctc
     1201 aatcgtaatc accccttctt caaacgaaat atggctacgg gtgtaaaact tatccgtata
     1261 tttcacataa tctttcttac tggcaactaa cagctcgtta cttccccaaa tatcatcaat
     1321 attgtgagaa aactgccctg ccaaaatatc caaaccattg gtatctttag aataatctac
     1381 tcgggtagat tttggcttct tagtcgattt attgctccca caagcaataa gaaacggaat
     1441 aattgccaac atcggcaaat atttagcata ttttttcatt gtgatttaac ctttattatt
     1501 cgaccggctt atagccttcg atgatgatct ctttaccttc aaataaaaaa ttgaccatct
     1561 cttgttcgag cagtttacgg tgttcggggt tcatcatact gagttttttc tcattgacga
     1621 tcattgtctg ttttttaatc cactcagacc acgcttgctt gctgattgag tcaaaaatac
     1681 gcttacccag ctcccccgga taaagttgaa aatctaaacc ttctgcctct tgttttaaat
     1741 attcgcaaaa tacggtacga gccataattt aatccttaat ctcatttttc acactgaatg
     1801 ccagctcatc taaaatacgt ttaaccggcg ttgctaaacc cacttcgtta tgttggtgca
     1861 aatcatacca ataatccgca tttgaagata ccttagaaag ataatttcct gctttttctt
     1921 cgacaacaag cggtgaattt tcacttgttt ttttcaaatc ggtttctact aaaaccgggg
     1981 taatatccaa atgaaaatgg ctaaaagtat gcctaaatgc agttaattgc tgtgaaattt
     2041 gcaaattttg cataaaatca gaccgcttga gagcctctaa gctctcaaat tgcggaaaca
     2101 caaataagcc cccccaaatg ccttttgctt cccgtttttg taacagcact ttcgagccgc
     2161 ttttcagcac taaaaaatag gtttgacgct ccggcaaggt ttttttcggt ttcttggcag
     2221 gaaagctatc ccacgcttgc attgcattcg ttttacaatt ttcttgtagc gggcagagca
     2281 agcacttagg ctttgtacgg gtgcaaatca tcgcccctaa atccatcatc gcttggttaa
     2341 agtctgccac ttgggtatca ggcgtcacac tttcgcttaa ttgccaaagg cgattttcca
     2401 ccgctttttc acccgcccag ccttctactg caaaatagcg gcttaacacc cgtttgacat
     2461 tgccatctaa aatcgggtgt ggggcatcta acacagaaga caaaatcgcc cctgcagtac
     2521 ttctgcccac tccggttaat gccaacacat catcaaatcg ggtaggaaat tcaccgccaa
     2581 actcatctcg aatttgaatt gccgctttat gcaaatttct tgcccgagcg taataaccca
     2641 gccctgtcca caaatgcaat acttcatcaa tcgaggcatt agctaagtca attacggtgg
     2701 gaaatcgttc cataaaacgt gcaaaataag gaataacggt cgctacttga gtttgttgca
     2761 acattacctc cgacaaccaa acctgataca gcgttttatt ttgctgccaa ggtaaatgtt
     2821 tccgcccata ttgctcaaac caagctaaaa cagctttggc aaacggatca gaagcttttg
     2881 ctttcgctaa aggaaatggg gaattgctgt tcattaaaaa tcttgaaatc cgaaaaataa
     2941 attatcatac tgcaagcggt cagttttacc aaaaatttta cacattaagg aaaaaagatg
     3001 aaatcgccca ttttaggttt ccaccacatt gcattaattg tttccgatta cgaaaaatcc
     3061 aaacatttct acacccgcat tttaggggca gagatcatcg cagaaaccta ccgagagcaa
     3121 cgtgccagct ataaattaga tttacgcttt aaagatggct cacaaattga attattcagt
     3181 tttcctgaaa cgccgcctcg tttcacctcg cccgaaacct gcgggcttcg ccatttggca
     3241 tttcgagtgg aaaatattga atatgccatc gcatttttag aacaacatca attaccgcat
     3301 gaagggatcc gaattgatga gatcaccggc aaacgcttca cgtttttccg agatccggac
     3361 gatctgccgt tagagtttta cgaggtctaa atgttatatt tactgattgc aaccctgatt
     3421 tgggcgagtt cattcattgt aggtaaagtg gcgatttcag cctttgaccc tgtccaaatt
     3481 atgcaaattc ggctgacgtt agcggcgttt atcgctctgc cgttttttat tcctgcgtat
     3541 aaaaaaatcc agaaaaatca gcacattaaa gtctggctga ttagttttct cacttttcct
     3601 ctgtacgtga tcttacaatt ttccggcttg aaattaactt ccgcagcaag tgccgtggca
     3661 ctactcgggc taaacccgct tttaactgtg ttagtcggct atttttgttt caaaaaacag
     3721 gcaaccaggc tcgattttat cctaagttta tttgcctgct tggggatttt aattatggca
     3781 tcgggtagcg atgatagcgg ttcaatcaat gtattaggat tattgcttgt aaccttaggc
     3841 agtttaagtt ttattgtggg aatgtacctc agcaaagaga ttctgcaatc agtaaaagtg
     3901 atcgatttaa ccaagatcat gctggtgcaa ggggcaatta ccgccttacc tattacctta
     3961 ctctttgcag aaaattggca actgccaacc agtctctcag catgggcgag cgtagcatat
     4021 ttaggcatta tttgtaccag cttggcaata ttgttatgga acaaaggcat cagccaatct
     4081 tcgcctattc ttgcaggtat tctactcgca cttgaaccgg tattcggact gctaatggca
     4141 ttggtctttt tatcagaaca gctcagttta gtgacttggg ttgggattat tattgttatt
     4201 tgcaccgcct tactctccgt tttgctaccg atgatgaaaa gatacaagcg gttaatttaa
     4261 caggattttt tgcaaatgaa atttccccct ctttcccacg gcattttact tcgccgttat
     4321 aaacgttttc ttgccgatat tcagttgcca aatggcgaac aaattaccct ccattgccca
     4381 aataccggtg cgatgacagg ctgtgccaac cccggtgata cagtttggtt ttccacctct
     4441 cataacccca aacgtaaata tgctcacact tgggaactaa cccaaaccca agcgggggat
     4501 tttatttgtg tcaatacctt gagagccaat cagcttgtac aagaagcctt ggaaaatcaa
     4561 tggattagcg agcttgcgga ttatcagcac attctgcctg aacaaaaata tggcagtgaa
     4621 aacagtcgga ttgatttttt gctcaaatcg gaccttctgc cggattgttt tgtggaagtg
     4681 aaatctacca cccttttaac cgaaaacggt gtgggaatgt tccccgatgc caaaaccgag
     4741 cgggggcaaa aacatttgcg ggagctgacg aaaatcgctg aacagggctt acaagcggtc
     4801 atttttttcg ctattttgca cacaggtatt gaacggtttg aagtggcaaa gcagatagac
     4861 cccaaatatg ccgaattatt cgctcaagcc attgcacaaa aagtgcaggt tctctcctat
     4921 aaaatggcaa tttcattgga aaacgggcaa cctattgcgg caaccttaac aaagaaatgt
     4981 gagttttaag cttgaaaaca cacagcatat ccccatataa gccaaagttt ttaaaaagaa
     5041 ggatttagta tgacaacgat tgtatgcgtg cgtaaagacg gaaaagtggc tatcggtggt
     5101 gatggtcagg caacgcttgg aaattgtatc gagaaaggca cggtgcggaa agttcgccgt
     5161 ttgtataaag acaaagtggt gacaggcttt gccggctcga ccgctgatgc ctttattttg
     5221 cgtgatttat tcgagaaaaa attggagtta caccaagggc atttagtgaa atcggcagtg
     5281 gaactggcaa aagagtggcg taccgagcgt gctttacgcc gtttagaagc gatgatgatt
     5341 gtcgccaatg acagcgagtt tttattggtt tcaggttcgg gcgatgtgat tgagccggag
     5401 caagatgtgc tggcgattgg ttcaggcggt aactatgcga aagccgctgc tcttgcgtta
     5461 ttacgcaccg aaaataattt atctgcaaaa gaaatcgttg cggaagcgtt aaaaatcgcc
     5521 ggcgatattg atatttacag taactacaat cacgtgattg aagaagttta gttaaataac
     5581 tctctcccct tgtgggacaa atgtttacat ttgaacgttg gctttgccaa cggctccaca
     5641 ggagcgagcg tagcgagtat tgagaccgaa aaatatttcg ttcagaaata tttttcagag
     5701 agaggggtta atgcaaaacg tttaagaaaa aagaccgctt gttaccctct ccctgatttt
     5761 tcttctgaac aaagaaaaat ctgtccctct cccacaaggg gagagggtaa ttaatttaag
     5821 gaataacatt atgtcaatga caccccgtga aatcgtctca gaattagacg cacacattat
     5881 cggacaaaaa gatgcaaaac gtgcggtagc aattgctttg cgtaaccgct ggagaagaat
     5941 gcagttgccg gaagatctcc gccaagaagt gacccccaaa aatattttaa tgatcggtcc
     6001 gaccggtgtg ggtaaaaccg aaatcgcccg ccgtttggcg aagcttgcca atgccccttt
     6061 tatcaaagtt gaggcaacca aattcactga agtggggtat gtgggtaaag aagtggattc
     6121 gattatccgt gatctagctg atgtgtcgat gaaattagtc cgccaacaag cggtcgaaaa
     6181 aaataaaatg cgtgcacaag atgcagcgga agatcgcatt ttagacgtat tattgccacc
     6241 ggcgaaagat cagtggggaa atgtgcagga atccgacaat aattctaccc gccaaacttt
     6301 ccgcaaaaaa ttgcgtgaag ggcaattaga cgataaagag attgaaattg acgtggcagc
     6361 tcaagtgagc gttgagatta tgaccccgcc gggaatggaa gagatgacct cgcaattaca
     6421 atcgctgttt gagggaatgt cgccaagcaa aaccaaaaaa cgcaaaatga aaatcaaaga
     6481 tgcgttaaag gtgatgcttg atgaagaagc ggcgaaatta gtaaatcctg aagagctgaa
     6541 acagcaagcg attgaagcgg tggaacagca cggtattgtc tttattgatg agatcgacaa
     6601 aatctgtaaa aaaggcgagc attcgggtgg agatgtttct cgtgaaggtg ttcagcgtga
     6661 tttacttcca atcattgaag gctcaacggt aaataccaag catggtatgg tgaaaaccga
     6721 ccatattctg tttatctgct cgggggcatt ccaagtggct cgcccgtctg atttactccc
     6781 agaattacag ggtcgcttgc cgattcgtgt ggagttaaaa tcgcttacca aagaagattt
     6841 tgagcgtatt ttaaccgaac cgaatgcctc attaaccttg caatatcgtg agttgatgaa
     6901 aactgaaggc gtggagattg agttcactaa agacggtatc agccgtattg cagaaagtgc
     6961 attccgtgtg aacgagaaaa ccgaaaacat cggggcaaga cgtttacaca ccgtgcttga
     7021 gcgtttgatg gacggcattt cgttcgatgc cagcgaacgc cgtggtgaaa aaatcatcat
     7081 tgatgaaaac tatgtttccg aagcgttaaa tgacgtggtg gaaaacgaag atttaagtcg
     7141 ctttattttg tagttttaag ttatacaagc ggttatttta gcgaaaaatc ttgcgaatta
     7201 gccgcttttc atattgttag ttgtaaaaat gaaaaatcta tacaaatggc ctgctgcaca
     7261 atcgctttat ccaaatcagg gaaagaagtc ttacgcaggg aaacgttttc gttaccgtct
     7321 tcgctcttgg ttgcatcatt ccaaaatcaa gcaatttgag cagttcgtta cacaaaatcc
     7381 acaactgatt ccgctcttaa atgctcgacc aaattacagc taccccgtgg ctcaccgctt
     7441 tttagataag cgtttcagtg caaaacaacg cttacaaaaa atcacagata acctgctgtt
     7501 tttaccgcaa aaattggctc atttaccacc tctatgggaa caagcggtca attttggtga
     7561 aattattgca gattttgagt tatggcttaa catcaatgaa catcagccga tggaaggttt
     7621 ttgggcatta gaacttcgcc acagaccgac taatcagcta gtttatttat taacatttgg
     7681 taagttagat gaagccttgt tgatagccgt aattcaaggt ccgaattttg aaggttcaaa
     7741 agagctggtg aaacaattaa ccaaaagttg ccacggctta cgcccggcct atttaatggt
     7801 tgaaacgatg aaggctttaa cggctgttct tggctatcag agtttactag gcattccaca
     7861 gaaataccag aataaatccc gctgggtgca aagtaagcgc tatgtagtgg attatgatgc
     7921 tatttttgcg gaatcgggcg gaaagccggg aaattattgg cagttacccc ttgaattgga
     7981 aagtaaaaaa atagatgaaa ttccaagcaa aaaacgctca atgtatcgca agcgttatga
     8041 aatgctagct aatttaaagc agaccatgga gcttatgctt aaaacttata ctggcttaaa
     8101 tgggtaaatc aaagaataca agcggtcaaa atctgcaaaa ctttgcaaaa ctttgcaaaa
     8161 aatgaccgct tacaagttac ttgccgccca tccatttatc atagattttt tgatattcgc
     8221 cattctcttt aatcgttttt aatgctgcat tgaattgatc aactaatact tggtttttca
     8281 agtgagttgc aatgcctaaa ccatcaccaa aatagtttgg attattgatt ttctccccta
     8341 caaaatctag cccttcgtgt tttcttgcca tctcagccaa taccggcaca tcgccaaaaa
     8401 tagcatcaat tcgcccattt tgcacatcta aaatagcatt ttgataagag gcataagatt
     8461 tcacattgta ctcttttttc tcttttgcta aatagtgttg gtaagtagtc ccattttgca
     8521 caccaatcgt tttcacctta gcaaaatctg tatctttttt cgcaatgaag gcagcagagc
     8581 ttggaaagta aggctcgcta aataatactt gtttcttacg tggttccgta atacccatac
     8641 ctgaaattgc agcatcaaat tgtttttgtt ttaggctttg gattaagcta tcaaaaggtt
     8701 ggctatggaa tgtacaattt gcattcatct ctttacagat agcatttgca atatccacat
     8761 caaaaccgat aatttctccc ttctcttcgg tcatttcaaa tggaggatag cttggctcca
     8821 tcacaaattt gatatcttgt gcctgcgcag taaccacaca cccgaataaa agggtcaaaa
     8881 gtgttttttt cataaaaagt ccctgtgttt tcattataag gattaccact ttaacgcagt
     8941 tactttctta aaaaaagtct tcttttcata aagtttgttt tatgtcatac aaacacatca
     9001 aattgagatg tagtttctca atcctcttga ttcctctatc tcaaaaaaac aacccaaaag
     9061 aaaaaagaaa agtatatgtt acattaatat tacaatgtaa ttattttgtt t
//
LOCUS       DANLXQ010000100         9058 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.100, whole genome shotgun sequence.
ACCESSION   DANLXQ010000100 DANLXQ010000000
VERSION     DANLXQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.100"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1076)
                     /locus_tag="PXD94_001800"
     CDS             complement(<1..1076)
                     /locus_tag="PXD94_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HDL6286641.1"
                     /translation="MMSFLSEVFSSFKPTKTEVISSEPNENSGESDIIQNKMSEVNNE
                     VQQLKRSGKLTQSQHHRRLYSQEPSFIDHLPWGEYLNEHGVILLDDARSVGAVFDIKP
                     IGTAGRSIEFLSRIRNLVKDALQDSFDELDVNPYVVQFYCQDSDDLQPYIEQLRNYIS
                     PIAKNSAFSEEWLRVQAKHFKDIGKPEGLFIDERVTNTAWSGRIRSTKMVIYRYVDKS
                     NDTNENLIVEQLNNACSRINGALTTAGLQLQRLDGEQFRQWLLRWFNPNPSINFEGLS
                     NNDCYHLFGKQTSNDGLPELAFNDFSEDLFYSQPKNEGNYWYFDDQPHEVVVVNNLRK
                     QPEVGLMTGEITRGRNINALFDLLP"
     gene            complement(1076..1474)
                     /locus_tag="PXD94_001801"
     CDS             complement(1076..1474)
                     /locus_tag="PXD94_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL6286642.1"
                     /translation="MKALYCIMMSALLITGCTTSTEELLPANGSTMRDIWDKGAGNND
                     LHLYRSQQGRTLDPVDYIPNTEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            complement(1487..2059)
                     /locus_tag="PXD94_001802"
                     /pseudo
     CDS             complement(1487..2059)
                     /locus_tag="PXD94_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
     gene            complement(2306..2491)
                     /locus_tag="PXD94_001803"
     CDS             complement(2306..2491)
                     /locus_tag="PXD94_001803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286643.1"
                     /translation="MNEQNNSWVQEWLNKSAKASERMATDEKFRQEVAKRATNSGQDI
                     QAILAKNIAKYQNQAKD"
     gene            2563..2712
                     /locus_tag="PXD94_001804"
                     /pseudo
     CDS             2563..2712
                     /locus_tag="PXD94_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
     gene            2713..3306
                     /locus_tag="PXD94_001805"
                     /pseudo
     CDS             2713..3306
                     /locus_tag="PXD94_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
     gene            complement(3460..4275)
                     /locus_tag="PXD94_001806"
     CDS             complement(3460..4275)
                     /locus_tag="PXD94_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDL6286644.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(4611..5447)
                     /locus_tag="PXD94_001807"
     CDS             complement(4611..5447)
                     /locus_tag="PXD94_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDL6286645.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5447..6250)
                     /locus_tag="PXD94_001808"
                     /note="aph(3'')-Ib"
     CDS             complement(5447..6250)
                     /locus_tag="PXD94_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDL6286646.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(6419..7264)
                     /gene="sul2"
                     /locus_tag="PXD94_001809"
     CDS             complement(6419..7264)
                     /gene="sul2"
                     /locus_tag="PXD94_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDL6286647.1"
                     /translation="MEYTSKSQIARNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQA
                     RKLMAEGADVIDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQ
                     AYALSRGVAYLNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHI
                     AAFFDARIAALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLL
                     SVSRKSFLRALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKE
                     TARIR"
     gene            7427..8719
                     /locus_tag="PXD94_001810"
     CDS             7427..8719
                     /locus_tag="PXD94_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL6286648.1"
                     /translation="MGTYIPHREGIIQTIVRNSLTQYQAYASPSQTILDRLEHFAECA
                     DWTKGVVRIRCHDCGHSYFRPFSCKVFHLCPSCDQKRTLLYAEYLAEDLLLDLPHRQF
                     VFTIPKILRPYFKSDKRLFGEVSRLVFSLLSDFFSLAAGQELLCACVVSYQSFGEFAR
                     FHPHWHVLVLEGGFTKYDRFVYLPIGADEGMLKVWQAAIMALFLRKELIDQARVNMLR
                     DWKHSGFSIESETRLFNKADREALGQYVVRGATCAEKIQYDPASDTIIWTASPKGFYK
                     GKTETFKGFEFVDQLVAHLPPRRVQLLRRYGVYAGKVRKQWQERPNIYSLAPESWQKG
                     HPRESKIVPAIPQEKPETVQVSDAWSKLRKQSWARLLQKVYEVDPFVCPKCQGTMSVV
                     AIIEDPKELAKIINWAKQQEREQPVAVCARSPPELALV"
BASE COUNT         2287 a         2275 c         2186 g         2310 t
ORIGIN      
        1 ggcaacaagt caaataatgc attgatatta cgcccacgag taatctcgcc agtcatcaac
       61 cccacttccg gttgtttcct caaattattg actacaacaa cttcgtgcgg ttggtcatca
      121 aaataccaat agttgccttc atttttcggt tgagaataaa ataaatcttc actgaaatcg
      181 ttaaacgcta attctggaag cccgtcattt gaggtttgtt taccaaaaag atggtagcaa
      241 tcattgttgc ttaacccctc aaaattaata gaaggattgg gattaaacca acgtaacagc
      301 cattgacgaa attgttcgcc atccagtcgc tgcaattgta atccagcagt tgttagagct
      361 ccatttatgc gagaacaagc attgttcagc tgctcgacaa tcagattttc attagtgtca
      421 ttggatttat caacataccg atagatcacc attttggtac tacgtatacg accagaccaa
      481 gctgtatttg tcactcgctc atcaataaaa agcccctctg gtttaccgat atccttgaaa
      541 tgcttggctt gtacccgtaa ccattcttcg ctaaatgcac tatttttagc gataggcgag
      601 atataatttc gtagctgttc aatataaggc tgtaaatcat cgctgtcttg gcaataaaat
      661 tgaacaacgt acggatttac atccaattca tcaaagctgt cttgcaaggc atctttgaca
      721 agattacgta tccgacttaa aaattcaata ctacggccag cagtaccaat cggcttaata
      781 tcaaatactg ccccaacact acgggcatca tcaaggagta tcacaccgtg ctcattcaaa
      841 tattctcccc aaggtagatg atcgataaag gagggctctt gtgagtataa ccgacggtga
      901 tgctgtgact gggtgagttt tccactgcgt tttaattgtt gaacctcgtt atttacctca
      961 ctcattttat tttgaatgat gtcagactca cctgaatttt catttggttc acttgatata
     1021 acctctgtct tagtcggttt gaaagaggaa aatacctcac ttaaaaaaga catcatcaaa
     1081 gccctcttgt tcgatcaccc ggttgagcat attggactcg cccgtagaaa ggtaataccg
     1141 tagaatatcc tggaatacta agaggctctc cggaacgagt ttggtgtgga tacacataca
     1201 taatcagatc cggattaggt aaacgaggga acaagttatt aatttcattt tctgccgtac
     1261 gggtataggc tcgttgctca gtgttaggaa tataatcgac aggatcgagt gttcgtcctt
     1321 gttgtgaacg gtataagtgt agatcgttat tacctgctcc tttatcccaa atatctcgca
     1381 tcgtactgcc gttagcgggt aataattctt cagttgaagt tgtgcaacct gttatcaata
     1441 aggcactcat cattatgcaa tataaagctt tcattgtttg ttccttttaa tcaagttgtg
     1501 tattagttga tgtgtttccg tatttcactt tacgtccttt ggagttgtaa tcgatctcaa
     1561 tctctttctc gatatgaata gcgacacgat gtccaggtgg tacatagaca gcatcaaagt
     1621 tttcagcaaa acgctttcta aaccagtcgg cggattcttt taaaccaccg ccgatggctt
     1681 gccctaagat gaacttacct tgatcaccgg taacagctcc aacaacacta ttgccatcaa
     1741 ctactgtcgt tgtttgtgat tgtgaaaacc cttgggctgc ggcactggca gccgctaaga
     1801 agaaatttgt gcctaaatat tctggtgcat ttgtttttct ctccccggga acacaaggaa
     1861 tgcctgaact atcagataac caaccgatat tctcactatt ccctttgtta gtcgtgctga
     1921 caatacgccc atcattgaac acaaaggtca tactttttac atcgcctcgt acacaagata
     1981 gagtccagtc gccagtagct gtacccgaaa caatcgcacc ttctacatca agtaattcaa
     2041 taccattggc aatgagatgg tttagcctta gttctcaatg cttttagctg attttctagg
     2101 atattttggg cgatttgctc caatcggtcg ttaaattctc gcaaaaactc catagtctgc
     2161 cgatactgtt ctgatgacaa tagattagaa tttgcgttat tttgcgtttt aggcgtgttt
     2221 gttggtgtgg gtgctacctt actaccattt ttatttttga taggtcttgt aggctctatg
     2281 agagcgtttt cagacgtttt attagttaat ctttcgcctg attttgatat ttggcgatat
     2341 tcttggctag aattgcctga atatcctgtc cgctgttcgt tgctcgtttg gcgacttcct
     2401 gacgaaattt ctcgtccgtt gccattctct cgctcgcttt cgctgacttg ttcaaccact
     2461 cttgaaccca agagttgttt tgctcgttca tctgattttt cctgtgtctg tgattgtgtg
     2521 gattttaagg tttggtaagc caaaacttgc tcgttatacg ccaaggagga cacccggcgc
     2581 aaggtgctgg taggcgcgtg gatactcggc aaggttgaaa gcggcgagtg gccagaacag
     2641 cgcctgctcg atgggttgga tagctaccta gagcgcgacc atgatcgggc gctgttcgga
     2701 ctgccaccca aggggtcgat ttgcggccac acctttgcgg taaatgaaaa aggtatcgtt
     2761 aacacggtga ataacatccg tgcagtgtat cgtccgcagg gattaccgcg tcagatcctg
     2821 gctcgcgcct cgctgaatgc gaacaccctc gacgaggcca tcactatact taccggccag
     2881 ccgcgatccg gggcgtttca ccatacgctc gggcagctgg gcgatgcgcg gctgttcagc
     2941 gttgaggcca ccggtcaagg ctgttccgtt ctacccctga cgacggtaac cgggcatgcc
     3001 aaccatctgg tgcatgcggc actggctggc gttgagcaga tcgttaccga cagttcagcg
     3061 tcgcggcagt tacgcctcgt gcagtggcgg gaaacgcaac ctccgtttga cgccgcggcg
     3121 gcaaaaacca ttctctcaga tacccatgac gcagaactgc caatttaccg gctggctgcc
     3181 gacgatccgg atgaggagaa caccctcgcc acggcagttt tcaccctcga tgccaaccac
     3241 gtcaggtggc aaattttcga cattaaccgc gacgatgcta aatttcatgg agaagtgcgt
     3301 gggtgaaata gccggtcgtc agtcataaag ggcaggggag taccgttggc ccggcgttcg
     3361 atagtaccgt ccggatactg ccagatatcg gcgtccaggg agatgtcctg aagcgcggtg
     3421 tccggaattt caggtttgtg tctctacaaa gactaactat cagaaaaact catcgagcat
     3481 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     3541 tttctgtaat gaaggagaaa attcaccgag gcagttccat aggatggcaa gatcctggta
     3601 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     3661 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     3721 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     3781 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     3841 gcgatcgctg ttaaaaggac aattactaac aggaatcgaa tgcaaccggc gcaggaacgc
     3901 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     3961 tgttttcccg gggagcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     4021 cttgatggtc ggaagaggca taaatgccgt cagccagttt agtctgacca tctcatctgt
     4081 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     4141 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     4201 cccatataaa tcagcatcca ggttggaatt taatcgcggc ctcgagcaag acgtttcccg
     4261 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     4321 tcatgatgat atatttttat cttgtgcaat ataacataag agattttgag acacaacgtg
     4381 gatttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     4441 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggc ccacctacaa
     4501 taaaggggtc gtttgcggga gagggcgaaa tcctacgcta aggctttggc caacgatatt
     4561 ctccggtaag attgatgtgt tcccagggga taggataagt cgcttgatat ctagtatgac
     4621 gtctgtcgca cctgcttgat cgcggccgcg atagctagat cgcgttgctc ctcttctcca
     4681 tccgcgttcc aagctgcgga aaggcaccca taagcgtacg cctggtcgag caggcgacgc
     4741 ggatcgacgt ccagcgcacg agagaatgcg tccgccatct gtgcaatgcg tctaggatcg
     4801 agacaaaggt cgtctctgtc agccggatcg tagaacatat tggcggcgcc aaagcccact
     4861 tcaccgacca gaccgacggg atctatcacc agccagccgc gactggagaa catgatgttt
     4921 tcatgatgca gatcgccatg tagcccacgc agttccgagg cattgctcat catttgatcg
     4981 gctataatcg ccgcgtggac gtagtcagtt tgacaacctg cgttttgatc atcgcgcgcc
     5041 cgctgaaaca aagctgcaaa gcgatcccgg atcgggagaa gggcagaagg caggggttcc
     5101 tcagatgcgg catacagctt cgccatcagt tccgctgcaa tttcggtcgc ctggtagtcg
     5161 ccgtgctcgg caacgatgtg agagagcatt cgctccccgg catattcgag caacatcaga
     5221 ttgttctcac gaccgagcaa ccggactgct cccctcccat tgcgccatac cagatagtcg
     5281 gccccgcgca gttcatcagc aatgtcttct ataggtttca atcccttgac gattgcagga
     5341 gtcccgtctg gcaatgaaac tttccaaacg aggctggaaa aggtgtccgc aatgagaaca
     5401 ggttgcgaaa cgtgccaatg agcaggaaaa acaggcggca tgaacatcaa ccccaagtca
     5461 gagggtccaa tcgcagatag aaggcaaggc gttcgcggtc gggggcttcg atccccaata
     5521 cattgaatag gacagcgaag gcgcgctctg cttcatctgg cgctgcccag ttctcttcgg
     5581 cgttagcaat catgagtgcc aaatcggcat agcgatctgc tgttccgagc cgcccaaggt
     5641 cgatcagacc cgtgcattga agagttttag ggtccaccat gaagttcggc atgcagggat
     5701 caccatggca aacaaccata tcggtgcgct cttggtcgag ccgcaccggt agctctcgtt
     5761 cgacacgagc caaaagatcg agctgcggcg tactcttgtc ctcgtccggt aagaagtcgg
     5821 gattgacggc attgcgggac accacatcaa cggcgcgtcc gaacattcgc gacagcctgc
     5881 gctcaaacgg acattgatca accgataggc tgtgaacagc gccaagttgc tgccccattg
     5941 acggccacgc tttgagcaaa tccgctccag acagatcagc cgccggtact cccggaattg
     6001 ccgttatcac caagcatgca ccctcctgtt cctcctgcca gttgatcacc tcggggcaag
     6061 ccacacctcg acctttgagc caaatgaggc ggtcacgctc tccagcgagc tcaccgcggc
     6121 gggaagcagg tgcgattttc gcgaaggcat gcccgtcacc acgtcgaaaa acaaaatcac
     6181 cggattctcc gcctctgaca ggcaaccagt cagaatgcga ttcaccaaaa aaaatattag
     6241 ttcgattcaa tggaggttcc ttcagttttc tgatgaagcg cggaggtggc tttgccacga
     6301 ataccatctg tgccgaaata tttgcgcacc ataaggtcga ttatcctgtc gtcgggtcgc
     6361 cctcaaaggg gacatgcctg ctgaaccgcg aatatagaga aatatcccga atgtgcagtt
     6421 aacgaattct tgcggtttct ttcagcgccg ccaataccgc cagcccgtcg cgcaaggggc
     6481 gcggctcgtg tgtgcggatg aagtcagctc cacctgcggc ggcggcaagc tctgcagcga
     6541 gtgtcgcggc cccgacatcc cccggaccac ggcctgtgag cgcgcgcaga aaggatttgc
     6601 gcgaaacaga cagaagcacc ggcaaatcga agcgcagccg caattcatcg aaccgcgcca
     6661 gcaccgagag cgaggtttcg ggagcagccc ccagaaaaaa ccccatgccg ggatcaagga
     6721 caaggcggtt gcgtttgata ccggcacccg tcagcgccgc gatgcgcgcg tcaaagaacg
     6781 ccgcaatgtg atccatgatg tcgccagcgg gtgcctcgcg ccgatctgcc tgcccgtctt
     6841 gcaccgaatg cataacgacg agtttggcag atgatttcgc caattgcgga tagaacgcag
     6901 cgtctggaaa accgcggata tcattgagat aggccacacc acgcgacaag gcataggctt
     6961 gcgtcgcggg ttgataactg tcgagcgaga cgggaatgcc atctgccttg agcgcgtcca
     7021 gcaccggcgc gatacgcgcg atttctgtgt cggacgaaac aggcgcggcg tcgggattgc
     7081 tggatgccgg accgaggtcg atcacatctg ccccctcggc catcagctta cgcgcctgcg
     7141 caatggctgc gtctggcgcc agataccggc ctccatcgga gaaactgtcc gaggttatgt
     7201 tgacgatgcc gaaaatgatg agcgatttat ttcgagctat ttgtgattta cttgtatatt
     7261 ccattttcct ccgcgccgaa ggcgtcaacc taactttcgg ttgagctgcg aacgggcttg
     7321 tcccgtgagt ccgctcgaac cggttgttag gtggcgccag tccacatttt ccattatttt
     7381 cttcgcaatt gcaagcaaat gatcgttttc ctgcgtatgt ataggtatgg gaacttacat
     7441 tccccatcgg gaagggataa tccagaccat agtccgcaat tctctaaccc agtaccaagc
     7501 ctacgcaagt ccatcgcaaa ctatccttga ccgccttgag cattttgctg aatgtgccga
     7561 ttggaccaag ggtgttgttc gaatacgttg ccatgattgt ggacattcgt acttcaggcc
     7621 attttcgtgc aaggttttcc acctttgccc atcctgtgac caaaaacgga ccttactcta
     7681 tgctgaatac ctggccgagg atttgctcct tgatctaccg catcggcaat ttgtttttac
     7741 gattcccaag attctcagac catactttaa atcggataag cgactgtttg gcgaggtgtc
     7801 caggctggta ttctccttgc tttctgattt tttctccctg gcagccggtc aagaactact
     7861 atgtgcctgt gttgtcagct atcagtcgtt tggtgaattc gctcgattcc acccgcactg
     7921 gcatgttctg gttctggaag gtgggttcac caagtacgac cgctttgtat atttaccaat
     7981 tggagccgac gaagggatgc tcaaggtctg gcaagcagcg attatggctt tgtttctgcg
     8041 aaaggaacta atagatcaag cccgagtgaa catgctcaga gactggaagc attccggctt
     8101 tagcatcgag agcgaaacca gactattcaa caaggccgat cgagaagccc tcggccagta
     8161 tgtcgttcgc ggcgctacct gtgcagaaaa aattcagtat gatccagctt ccgacacgat
     8221 aatctggaca gctagcccaa agggattcta caaaggaaaa acagagacct tcaaaggctt
     8281 tgaattcgtc gaccagcttg tggcccattt accaccaagg cgggttcaat tgcttcgtcg
     8341 ctatggcgta tatgcgggca aagtccgcaa gcagtggcaa gagcgcccca atatttatag
     8401 cctggcccca gaaagttggc aaaaaggtca tccacgcgaa tccaaaattg ttccagccat
     8461 acctcaggag aaaccggaaa ctgtccaagt ttccgatgcc tggtccaagt tgcgcaaaca
     8521 aagttgggca cggttgttac agaaagtcta tgaagttgac ccgtttgttt gcccaaaatg
     8581 ccagggaaca atgtcggttg tggcgataat cgaggatccc aaggagcttg ccaagattat
     8641 taactgggca aagcagcagg aacgggagca gccagtggcc gtctgtgctc gttcacctcc
     8701 tgagcttgct ttggtgtagg tataataccc aaaaacgaag tttatgcgga acaggataac
     8761 acctcgaaat cggagtttat atggcttcgt gggggagtta tgtgtttgag ctttgtcgca
     8821 ttaacgcgaa tcaggggttg acggcagaaa tagactggaa ttacacttct tggagtcggc
     8881 gttgccggaa aattctgatt ggattagttg ttcggggtgc gaaaacagtc gtagttcggg
     8941 gaaaaaaccg aattttgacc cgaaaccgca aaaaacccga aatgacagtt cctataattt
     9001 atttatttgg gggcgtgcac ctgtagcctt agcgacttta aatgacagtt cctataat
//
LOCUS       DANLXQ010000101         8983 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.101, whole genome shotgun sequence.
ACCESSION   DANLXQ010000101 DANLXQ010000000
VERSION     DANLXQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.101"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..773
                     /locus_tag="PXD94_001811"
     CDS             <1..773
                     /locus_tag="PXD94_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6286649.1"
                     /translation="TSAGTLLKELFETKLPVFSAPKNPNFLRHLITLFEDKNFISLDF
                     FAGSSSFPHAILESNRIDKGNRKFIAVQYPEEIDIKSKNGKVAKQFCQRKNLPLYITE
                     ISKERIRRAGKQILQNNPENRPLDVGFKVFKLTESHFKQWQSPTSENLADQLEMFIDP
                     VSEKADTQAVLYELLLRLGLKLTAKVRLENKVFWVEENGLIFALLLNAADEEIIQTVI
                     AQQPKKVIALDRLFNGNDARKKNTELQMQDAGITFFVI"
     gene            783..3419
                     /locus_tag="PXD94_001812"
     CDS             783..3419
                     /locus_tag="PXD94_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL6286650.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQTSRLKSF
                     ASANHIEILVMNIDAFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEILFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     EATKIKHSKVKLEMNETGISTNYRSSNETAVNTQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEKINLALQQIMTDGVEYHKIAGKTYEMTLFGDFDFY
                     LNQYTFEVQDKTKTIYEDYIALDSQTENIFARHCESSEQVEFYFKLPKRFKIPTPLGS
                     YNPDWAVVFKGENKIYLVAETKNTGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRD
                     YKDLDYRVVQKVEELSAEYCKI"
     gene            3551..5983
                     /gene="gyrB"
                     /locus_tag="PXD94_001813"
     CDS             3551..5983
                     /gene="gyrB"
                     /locus_tag="PXD94_001813"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL6286651.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLQWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(6036..6998)
                     /gene="cmoB"
                     /locus_tag="PXD94_001814"
     CDS             complement(6036..6998)
                     /gene="cmoB"
                     /locus_tag="PXD94_001814"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL6286652.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLVPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(7095..8858)
                     /locus_tag="PXD94_001815"
     CDS             complement(7095..8858)
                     /locus_tag="PXD94_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDL6286653.1"
                     /translation="MSEQTFITGKDAALEDSIATFQHKLKTLGFNIEEASWLNPVPNV
                     WSVHIRDADCPQCFSNGKGASKKAALASALGEYFERLSTNYFWADFYLGQEIANSEFV
                     HYPTEKWFPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYV
                     RQSDNETVYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLP
                     EIPQSVIDGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNPNNGTCFASFG
                     AHPNFQVAFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWD
                     LFKRDADYPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMS
                     NIYPSDDLVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFI
                     GVVAEKGSAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCL
                     IQSIELFLDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTA
                     HQKLLNAYRKVQKAKVNPIAR"
BASE COUNT         2861 a         1734 c         1901 g         2487 t
ORIGIN      
        1 caacttcggc aggaacactg ctaaaagaat tattcgaaac aaaactccct gttttctctg
       61 cacctaaaaa tcctaatttt ttaagacatt taataactct atttgaagat aaaaatttta
      121 tttctttaga tttctttgct ggcagttcat catttccaca cgcaatattg gaaagtaata
      181 gaattgataa gggtaataga aaatttattg cggttcaata tcctgaagaa attgatatca
      241 agagtaagaa tggaaaagta gcaaagcaat tttgccaaag aaaaaattta ccactatata
      301 taactgaaat ttcaaaagaa cgtattcgcc gtgccggaaa gcagattttg caaaataacc
      361 ccgaaaaccg accgcttgat gtaggtttta aagtcttcaa actgacagaa agccatttta
      421 aacaatggca atcgcccaca tctgaaaatt tagccgatca acttgaaatg tttattgatc
      481 ctgttagtga aaaagcggat acgcaagccg ttttatatga gcttttattg cgtttagggt
      541 taaaactcac ggcaaaagtg cggttggaaa ataaggtgtt ttgggtggaa gaaaacgggc
      601 tgattttcgc cttgctgtta aatgcagccg acgaagaaat tattcaaact gttatcgctc
      661 aacaaccgaa aaaagtcatc gctttagacc gtttattcaa cggcaatgat gcccggaaga
      721 aaaacaccga attgcaaatg caagatgccg gcattacgtt ttttgtgatt taaggagaaa
      781 aaatggaact ccaatttgaa accttagagt atcaattaca agccgttaat gccacagtaa
      841 atttgtttgt cggacagccg aatgccgcaa ctgaattttc actcaaagcc caaaatgata
      901 tgcggtttgt gccgaattta ggcttgcaga ttagtgatga gcagttacaa cagaatttgg
      961 ctaatctgca aaacaggcaa aaaatcgacc gcactttgct tgtcgaacag ggaaaaaatt
     1021 ttaccgtgga aatggaaact ggtacaggga aaacctatgt ctatttacgc acgatttttg
     1081 aattaaaccg tcaatacggc tggcaaaaat ttgtgattgt cgtgccgagt gtggcaattc
     1141 gtgagggagt gttgcatacg ttggaaacta caaaaagcca ttttaatacg gtatttgata
     1201 acccgagcgt caatcaaaaa tttgaatata aaagtaacca aacttcacgc ttaaaatcct
     1261 ttgctagtgc taatcatatt gaaattttag tgatgaatat tgatgctttc accaaagaaa
     1321 gtaacgtgat taataccgtg aatgaaagcg gtgatgctcc gattttctat attcaacagg
     1381 cgaatccgat tgtgattatt gatgaaccgc aaaatatgga aacggagatt cgccgtaatg
     1441 cgattgagag tttaagtccg cttttcactt tgcgttattc tgccacccat aaaaatgcgt
     1501 ataacccgat ttttagcctc aatccggtgc aagcctatga aatgggatta gttaaacaaa
     1561 ttgaggtaga tagcgtattg gcggaaaatg aggtaaatgg agcttatctt tccctcaaag
     1621 agattaaaac aggggcaaaa agctggtcgg caaaaatcga aattttattt aacgataaaa
     1681 aagcgatgaa aaagaaagtg attagcgtga aaccgaatca ggatttgtat gagctttcca
     1741 accaaaatga aatgtatcgt tcagggttta tattaagcgg aatggatacc gatagccaaa
     1801 tggttagctt ctccaacggc acacaaattt ttcaaggggc ggaacattcc caacttaaag
     1861 aagatgtgca aaaaatgcag attcgccgca ctattggcga gcatttgcaa aaagaaaaga
     1921 aattacgacc gcttgggatc aaggtgttgt ctttgttctt tatcgataaa gtggataatt
     1981 atcgcccttc tgcacaaagc cctacggggg gaaaatttgc aaaatggttt gaggaaattt
     2041 atttggagct agccaaagaa gagcctcaag gcgtgcataa cggttatttc tcacaggata
     2101 aaaaaggcgt agcaaaagat actaatggca caacgcaagc ggataacgag acttatcaac
     2161 tgattatgcg ggataaagaa aaattgcttt cgctggagaa tccgttacgg tttattttct
     2221 cgcattctgc cttaaaagaa ggatgggata atccgaatgt gtttcaaatt tgtactttaa
     2281 acgatactgc aagcgaaatg aaaaaacgcc aagagattgg gcggggcttg cgtttgcctg
     2341 tgaatcaaca aggattgcgg atttatgatc gtcatctaaa tacgctgaca attatcgcaa
     2401 atgaaagtta cgaacaattt gccgccacat tacagcgtga aattgaggaa gattgtggtg
     2461 tacagtttgc caaaagcaat gttaaaaaac gggaagataa aaaagccgtt cgctaccgta
     2521 aagatttcag cttacatccg gaatttgcgg caatttggga acggattaaa caccaaacca
     2581 cttatcgggt agatttcagt aatgcggaat tgattgaaag tgcggtggaa aaaatccgca
     2641 aaatgcccga tatcgaggcg acaaaaatta agcattccaa agtgaaattg gaaatgaatg
     2701 aaacagggat ttcaactaat tatcgctcat ctaatgaaac agcggtaaat acacaatggg
     2761 cgatacctga tttattgagt gagattcaaa agaaaaccgg gctgacgcgt gcgacccttt
     2821 gcaaaatttt acaaaaatcc gaccgcttgc aagaagtctt ccgcaatcct caacgcttta
     2881 ttgatttaat tgcagagaaa atcaaccttg ctttacaaca aattatgaca gacggggtgg
     2941 aatatcacaa aattgccgga aaaacctatg aaatgacgtt attcggcgat tttgattttt
     3001 acctcaatca atatactttt gaagttcaag ataaaacaaa aacgatttat gaagactata
     3061 ttgcactgga ttcccaaaca gaaaatatct tcgcccgaca ttgtgaatct agtgagcaag
     3121 tggagtttta ttttaaatta cccaaacgtt ttaaaattcc aacgccatta ggcagctata
     3181 atcccgattg ggcagttgtg tttaagggag aaaataaaat ttacttggtt gccgaaacga
     3241 aaaatacagg caaaggtatt caagcaggag tagatgaaga aaaattagat gaaagtgaac
     3301 gaatgaaaat cgactatgcc cgtaagcatt tcaaaggctt acagagagat tataaggatt
     3361 tggattatag ggttgtacag aaagtggagg agctgtctgc ggagtattgt aaaatttaat
     3421 ctgtggtctt gtagaattga gatagactgc aatttcaact aaaatttaac cgcttgtttt
     3481 gcgatacttt tacgcatttt tctgctataa tcggcacaat tttttcgatt tcaaattagt
     3541 aggaataaga atgtctgaac aaactcaagc aacaagttat gattcttcca gtattaaggt
     3601 gttacgcggc ttagatgcgg tgcgtaaacg cccgggaatg tatatcggcg ataccgatga
     3661 cggtacgggc ttacaccata tggtgtttga ggtggttgat aatgcgattg acgaagcatt
     3721 agccgggcat tgtaaagaca ttattgtcac aattcattcc gataattcgg tttctgtgca
     3781 agatgacggt cgtggaattc cggtggggat tcacccggaa gaaggcgttt cggcagcaga
     3841 agtcattatg actgttcttc acgcaggcgg taaatttgac gataactcct ataaggtttc
     3901 cggcggtttg cacggtgtgg gcgtatcggt ggtgaatgca ctttcctcta aattacaact
     3961 cactattcgc cgtgaaggac acgttcacga gcaattctat agcttaggtg agcctgatgc
     4021 acctttagcg attatcggca ataccgaaaa aacggggacg tcggttcgtt tctggccaag
     4081 tttggatatt ttcaaaaata aaaccgaatt tgaatataaa attctgtcta aacgcttgcg
     4141 tgaactatca tttttgaatt caggcgtttc gattaaatta attgatgaac gagacggcaa
     4201 agaagagcat tttaaatatg agggcggtat taaagcctat gttgagtatt taaacgaagg
     4261 caaaactcat attcacaata cgccattcta tttgaccact gagaaagacg gcattggcgt
     4321 ggaaatttcc ctacagtgga atgacagtta caacgaaaat gtgtactgct ttaccaataa
     4381 tattccacag cgtgatggcg gtacgcactt agccggtttc cgtggggcat taactcgtgc
     4441 gttgaaaaac tatatggata acagcggggt attgaaaaaa gccgatgcga acattgatgc
     4501 ctcaggtgat gatgcccgtg aaggtttagt agcggtgatt tcggtgaaag tgccggatcc
     4561 gaaattctcc tcacaaacca aagataaatt ggtgtcgtca gaagtgcgtg gagcagtgga
     4621 aagctcaatg aatgaggctt tatccgacta tttagccgaa aatccggacg atgcaaaaaa
     4681 tatcgttacc aaaattattg atgcagcccg tgcaagagaa gcagctcgta aagcccgtga
     4741 aatgacccgc cgtaaagggg cgttagattt aggcggctta ccgggtaaat tagctgattg
     4801 tcaggaaaaa gatccggcgt tatcggagct ttacttggtg gagggtgact ctgccggtgg
     4861 ctcggcaaaa caagggcgtg accgtaaaaa ccaagcgatt ttgccgttaa aagggaaaat
     4921 tctgaacgtg gaaaaagccc gctttgataa aatgctttct tcacaagaag tagcaacctt
     4981 gattaccgct ttaggcacag gcatcgggcg tgatgattat aatttggaaa aattacgtta
     5041 ccacaaaatc attattatga ccgatgcgga cgtggatggt gcacatattc gtacgctttt
     5101 attaactttc ttctaccgtc aaatgccgga gctgattgaa aacggctata tttatattgc
     5161 acagccaccg ctttataaag tgaaaaaagg caagcaagag cgttatattc aagataacga
     5221 tgaaatgtcg caatatgaaa ttgatattgc attagaaggt gcaggcttat atgtgagtga
     5281 agatgctcca gcgttaagtg gcgtagcgtt agagaactta attgcccaat ataacggcgt
     5341 acaaaaatta attgaacgtt taagccgtcg ttacccaaca ttattactta acgaattaat
     5401 ttattcaaaa ccgctttcgg tggaatttgc aaaaaatcaa gcaaatatga ccgcttggag
     5461 cgagcaattt gtcgctacat tgatggcaaa agaggtgggc ggcagtttct accgtattaa
     5521 taccttgttc aatgaagaac gccaaattta tgagccggaa attattgtga ctacacacgg
     5581 tatggacagt acctatcgtt tagattacaa ctttatcaac ggtaatgaat acgctcgcat
     5641 tgtggcatta ggcaacgagc tgaacgattt actatctgat tcagcctatg tggtgcgtgg
     5701 tgaacgtcgc caagaaatca gcagttttgc tgaagcctta gattggttgg tgaaagaatc
     5761 tcgtaaaggc ttaactattc aacgttataa agggttaggt gagatgaatc cggaacaatt
     5821 atgggaaacc acgatggatc cggtggctcg taaaatgcta caggtcaata ttaccgatgc
     5881 gattgcggca gataaactct tcagtacgct gatgggcgat gaagttgaac cacgccgaga
     5941 tttcattgaa actaacgctt tgtatgctca gttagatatt tagttaaaaa atccgcattt
     6001 gacatgcgga ttttttattc tcttaaacta gcattttaag cattcgctaa aatcaccgcc
     6061 cgcttcggag cagggtagcc ttcaatggtt ttgctatgat cgtttggatc taaaaaatcc
     6121 actaaacttt cattttccaa ccaatcggtt tttcgctgct cttctagtgt agtgattgcc
     6181 tcatcaacac aacgcacatt tttaaagccg actttttcca gccaattaat gagtgcagca
     6241 acagaaggaa taaaatagat atttttcatt ttcgcatagc gatcagccgg cactaaaaca
     6301 tcattaatat cgccatcaat caccagcgtt tctaacacca attcaccgcc tctgactaat
     6361 tgggctttga gttgagaaag atgatcaagc ggtgatttgc gatgataaag cacccccatt
     6421 gaaaacacgg tatcaaaagc agcaagcggt tgcatttgct caataccaag tggaatcaga
     6481 ttagcccgtc tatcattccc taagagtttc cgcactgcct caaattggca caggaacagc
     6541 tcggtcgggt caataccaac taccattttt gccccttcgc ctaccattcg ccacatatga
     6601 taaccgctac cgcagccgac atctaaaatg gtgcggtctt ttaacggtgc aagatgcgga
     6661 agcacacgat cccatttaaa atcggaacgc cattcggtat caatatgaat cccgtgtaga
     6721 tgataagggc ctttacgcca cggcattagt tgctttaaat ggtgggtaat ttgcttgact
     6781 tccccctcag ataaaggctt gcttgaaata gactcaacct tgtcttttag attgatacaa
     6841 gcggtcgaat ttggtaaaaa atctgcaact tttgcccact tggcatagtc agcgtgggtg
     6901 gtcttttccc attgtttgag ttgtaagggt aaggtttcta accaagcaga aaggtcggtt
     6961 gtggcaattt gttgataaaa aggacgaaag tcaatcattt tgaaatttat gtctagggga
     7021 gagacctatg ccctcccgaa aggtagtaaa tagaaaggac aggcacagaa cctaccccta
     7081 cgaaaaatat aaaattatct cgcaatcgga tttactttcg ctttttgcac tttgcgataa
     7141 gcgttcagta atttctgatg tgcagtcata ttttcaagcg attcgtccga tgcagttaaa
     7201 ccgtggaacg ggttaccgcc ttggattgca ccccaagcag cttcaaccgc ttgtttaccg
     7261 tacattttct caaacaccat acgatattgt tccgaatcac gctcttcatc taagaacaac
     7321 tcaatcgatt ggattaagca gcggtaataa ttgttacgtt ctgctgagaa tacgcttgca
     7381 ttcatattcg ctgtccagtt tgcccagtct gaggcttgct ctaagttacc taaagctaaa
     7441 tgcagcatcg atttaagctc accgatacgc aatgtttgca ttgcagagcc tttctctgcc
     7501 accacgccaa taaattcacg cacacgggtt aaatcatcaa tgctttgctc atctaattca
     7561 tcaagcagtt cttggtaagt ttcagcatcg tgatggaaat gtggcaaatc taacaagatt
     7621 tcacgccaat ccatacccat attgttattg gcatagacca aatcatcgct tggatagata
     7681 ttcgacatac ccggtgcaag aatacggcaa gcgtacacac ctaaatgctc ataatccata
     7741 atatacacag gctgattatg cttgttgaaa atcgccatca tattattaaa ttcttgctcc
     7801 gttgtgccgg agaagtccca atgcacgaac ggataatccg catcacgttt gaaaagatcc
     7861 cacgaaatta gaccgcttga atcaataaag tgtgtctcaa ggttcgcatg atctgctaca
     7921 tcttcattat taaatgaagg tggggcaaac acatctaaat cctttaagct acggccttgt
     7981 aataactcgg ttacggtacg ttcaaacgcc acttggaaat tcgggtgtgc accaaaagag
     8041 gcaaaacaag taccgttatt tgggtttaac aagatcacac aaataactgg aaattctccg
     8101 cctaatgaag cgtcatagca taaaatcggg aagtcttctt gctctagttt ctcgatagag
     8161 gctttgattg tcggataacc gtcaatcaca ctttgcggaa tttccggcaa gctgattgcc
     8221 tcagtaataa tacggttttt gacaaaacgc tcgaacactt cggataagcc ctgcactcga
     8281 gcttcatttt ttgtgttgcc tgccgacata ccgtttgaaa caaacaggtt gccgataatg
     8341 ctttgcggaa tgtaaaccgt ttcattgtca gattggcgaa cgtaaggtag ggtaacaatt
     8401 ccacgatcat aatttcctga ttgtaaatca accaataatt ccggcgtgat ttcaccattc
     8461 ggatcaaaat attcccataa aaactcgtcc atcatctcca ttggtggaat ggcttcatca
     8521 tcaatcggaa accatttttc ggttggataa tgcacaaatt cactattagc aatctcttga
     8581 cctaaataaa aatccgccca gaaatagttg gtagataaac gctcaaaata ctcacctaat
     8641 gcagaggcaa gagcggcttt tttgcttgct cccttaccgt tagaaaaaca ttgcggacag
     8701 tctgcatcac gaatatgcac cgaccacaca ttcggaacag gatttaacca agaggcttct
     8761 tcaatattaa aacctaaggt ttttaattta tgctgaaagg tcgcaatgct gtcttcaagt
     8821 gcagcatctt taccggtaat aaaagtttgt tctgacatga taataccttt aacttgtcgt
     8881 aaaaatggca acattctagc aaaaatcccc atagataagt aaattttttg tgaaatttct
     8941 gctgaaagaa cagatttcaa aacttagaaa aaatgattac aag
//
LOCUS       DANLXQ010000102         8982 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.102, whole genome shotgun sequence.
ACCESSION   DANLXQ010000102 DANLXQ010000000
VERSION     DANLXQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8982
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.102"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            54..1160
                     /gene="mltA"
                     /locus_tag="PXD94_001816"
     CDS             54..1160
                     /gene="mltA"
                     /locus_tag="PXD94_001816"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL6286654.1"
                     /translation="MNWKAYKKAIAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKF
                     GAKYNGRHFMPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKIS
                     RWISSGGNINDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAM
                     PSSKRFSRAEIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQ
                     NGFKYASVGRLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTG
                     QVKGSAGVPLVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGG
                     AVKGHHFDLYQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            1173..1622
                     /locus_tag="PXD94_001817"
     CDS             1173..1622
                     /locus_tag="PXD94_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286655.1"
                     /translation="MLKKLSAIFLLLPSAVLANNLQNITASTEPKYKISEIDVRILIR
                     QLNNIEQCIYPELAKPGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTL
                     STEYFHLLHSRLNHQKANVDPEKCDTFKPRYKEIYKSMENALTKKNQ"
     gene            complement(1656..3173)
                     /gene="purF"
                     /locus_tag="PXD94_001818"
     CDS             complement(1656..3173)
                     /gene="purF"
                     /locus_tag="PXD94_001818"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL6286656.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIHNER"
     gene            complement(3188..3691)
                     /locus_tag="PXD94_001819"
     CDS             complement(3188..3691)
                     /locus_tag="PXD94_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL6286657.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            complement(3837..4298)
                     /gene="rnhA"
                     /locus_tag="PXD94_001820"
     CDS             complement(3837..4298)
                     /gene="rnhA"
                     /locus_tag="PXD94_001820"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL6286658.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            complement(4391..5722)
                     /gene="argG"
                     /locus_tag="PXD94_001821"
     CDS             complement(4391..5722)
                     /gene="argG"
                     /locus_tag="PXD94_001821"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL6286659.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            5906..6946
                     /locus_tag="PXD94_001822"
     CDS             5906..6946
                     /locus_tag="PXD94_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6286660.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKIIIDTANKKDKNEFNRILQAVKNEQFDAVICFVSDWHNAKLTWQSGIK
                     YRLAPATKLFQFLYNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSV
                     EQQTVQAQKAKLCEQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYV
                     VLTAGKGESEKAQQLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISG
                     ALNIPTIGFYPSRLSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIV
                     PFIRRMWNTPSL"
     gene            complement(7011..7769)
                     /locus_tag="PXD94_001823"
     CDS             complement(7011..7769)
                     /locus_tag="PXD94_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL6286661.1"
                     /translation="MPTLSVAMIVKNEAQDLRACLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            7896..8210
                     /gene="yhbY"
                     /locus_tag="PXD94_001824"
     CDS             7896..8210
                     /gene="yhbY"
                     /locus_tag="PXD94_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL6286662.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            8271..>8982
                     /locus_tag="PXD94_001825"
     CDS             8271..>8982
                     /locus_tag="PXD94_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL6286663.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLARVVRKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFA"
BASE COUNT         2691 a         1909 c         1697 g         2685 t
ORIGIN      
        1 ttgctattct tctgtttatt tttcacattt tattaacgat ataaggatat tcgatgaatt
       61 ggaaagcata taaaaaagct attgcggcag ctagcgtggt agcgattgca acggtattag
      121 tggcgtgttc gtcttcaggc caaaaaggca aaacaacctc ttatgatgcg gaaaaagcta
      181 aatttggagc aaaatataat ggtcgtcact ttatgcctta tggcacacat tttcaagctg
      241 gacgtttagg attaaatgct gctcctgtta atcaactaga ctttttacag cagctttcta
      301 atgtcagagc ctatagtagc ttggcagatt cttatgctat cgcttacgga aaaatcagtc
      361 gttggatctc atcgggcggt aatattaatg atttagcaaa atatggtatt agtactcgtt
      421 taatgcgtgg cgaagatggc taccagaatg tgttaatgac agggtattac tcgccggtta
      481 tcaaagcccg tagaacgcct caggggcaat accaacaccc gatttatgcg atgccgtcta
      541 gcaaacgttt ttcacgggcg gaaatttacg caggagcatt gcaaggtcag ggcttagaac
      601 tggcttatag taattcaatg ctcgataact tcttattagg tgttcaaggt agcggttacg
      661 ttgactttgg tgatggtcgc ttaaactatt ttgcttatgc agggcaaaat ggctttaaat
      721 atgccagcgt tgggcgttta ttagtagaag atggcgaaat tccgaaagag aaaatgtcag
      781 tgcaggctat ccgtgaatgg gggcaacgta accctagccg tgttcaaggt ttgttggagc
      841 gtaatccatc gtatgttttc tttaaaaacg atcctaccgg tcaggtaaaa ggctctgccg
      901 gtgtgccgtt agtaccactt gcatctgttg catcagatcg tagcattgtg ccatcaggta
      961 gcgtgttgtt ggtcgaaatg ccgttgattg atagcaaagg aaattggacc ggcaaacacg
     1021 aaatgcgttt aatggtggct cttgatgttg gcggtgcagt gaaaggccat cacttcgatt
     1081 tatatcaagg gattggcgat aaagcaggcc atatcgcagg tttaatgaaa cattacggcc
     1141 gtgtttgggt attaaattaa tacagggtaa ctatgttaaa aaaactatct gccatattct
     1201 tacttctacc gagcgccgtg cttgcaaata atttgcaaaa tataaccgct tcaactgagc
     1261 ctaaatataa aatctcagaa atagatgtca gaattttgat acgccagctc aataatattg
     1321 agcaatgtat ctatcctgag ttagctaaac ccggttacca gcaaatctac gcaaattgga
     1381 atctggctga aaatttaacg atgcaatatt ttgaatatca gcttctaaaa gagttgctcg
     1441 gagaggaaaa tcagaaactg atgcaaaatg atacgctttc taccgagtat ttccatttac
     1501 tgcattctcg tttaaatcac caaaaagcaa atgtagatcc cgaaaaatgc gatacattta
     1561 aacctagata taaagagatt tataaaagta tggaaaatgc actcactaag aaaaatcaat
     1621 agtaaaaaac tcgcagtttt cactgcgagt ttcttttatc tttcgttatg aatttctaaa
     1681 ttggtggctt gttttgccgc ctctgctttt gctttatcgt ttcgagatct tgctatactg
     1741 tctaaatagc atttatctac atcacctgta atatattctc ctgtaaatac agagtcatca
     1801 aagcgatgaa tagtcggatt ttccagttga atagacttat aaagtgcttc aaggtcttgg
     1861 aaaatcaatt tatccacccc tatcatctgt gcaacctctt ccactgagcg atcataagcc
     1921 actaattctt cacaagtcgg catatcaatg ccatacacat tcgggtagcg aatttccggt
     1981 gcggcagagg caaaataaac ccgttttgca ccagctgctc gtgccatttc cacgatttgt
     2041 tcagacgttg tacctcgtac aatagaatca tcaaccagta acacgctttt gcctttaaat
     2101 tcagaggcaa tcgcatttaa tttacggcga accgagcttt tacgctgtgc ttgccccggc
     2161 ataataaaag ttcgagctac atagcggttt ttaacaaacc cttgacgata gggtttatac
     2221 aacatattag caatacgtac cgcaatatca tttgaggttt caggaatcgg gatcaccaca
     2281 tcaatatcat cgataattcg tccccattca cgtttaattt tctcacctaa taattcgccc
     2341 atatgcactc gtgcagaata tacagaaacg ccatcaatga cggaatcagg acgggcaaaa
     2401 taaacatatt caaaaataca aggattcagt tttggattat cggcacaaat ttgcgaatgt
     2461 aattgcccat caaaggtaac ataaatcgct tcacccggca gcacatctcg cacaaattca
     2521 aaccccacta catcaagagc cacactttcc gaagcaaaca tatattcggt tttaccctcg
     2581 atttcacgtt tacccagcac taacgggcga ataccgaatg ggtcacggaa agcaaccata
     2641 ccgtgtccga taatcatcgc aatgcaagca taagcaccac gaatgctatc attggtttta
     2701 cgaaccgttt caaagatatt gtctgggctt aaatgctgag tggaatagag atctaaaaag
     2761 taagcaaaaa tattaagaag ggattcagaa tcagaattag tgttcacatg gcggcgggct
     2821 tcgttgtata agcgagcttt aagttccgca ttattagtta aattaccgtt gtgaactaag
     2881 gtaataccga aaggtgaatt gacataaaat ggctgggctt cagacacact tgagctacct
     2941 gctgttgggt agcgaacgtg accaattcca acattgcctt gtaatctcac catatgctct
     3001 tgctggaaaa catcgctgac taagccgtta gctttgcgta agcggaaacg attttcatcg
     3061 tctatggtga cgatacctgc ggcatcttgt cctcggtgtt gaagtaatgt taaaccatca
     3121 taaatcgcct gattcaccgg cgaattccca ataataccga caatgccgca catatagaaa
     3181 aacctcttta tttagtcgtg tttaaaaaac tggaatttgc ttgtaactgt tcaaagaacc
     3241 acttcacaat aaagtcaaaa tgcggaatta atagcgatga tttccataat tccgtttggc
     3301 ttgcttgggt aaaggtatct aagaaaaata aaatagccga cacaattaaa ataccacgca
     3361 atgccccaaa agcaccgcct aaaatacggt cggtgataga cattccgctc aaatcaatca
     3421 ctttgcctaa taaaccgttg atcacaccac aaacaattaa agtcaaaatg aacagaatcg
     3481 ccgctgcaat accattgcgt aaaaattctg aattttgcaa ataaaccgaa ttaatttgtg
     3541 tgagataacc gcttaaataa ggataaaacg agcttgccac aaaaaaggcg attacccaac
     3601 caagtagcga taatgcttca ttggtaaaac ctcgccataa actgataagc actgaaaaag
     3661 caatgattcc gataacaatt aaatcaacca taacacacct taaagcgtaa agctctccta
     3721 ttctacaaaa aaagaaaacg tttgcgtagc actatttcgc taaaaataag tgctttatgc
     3781 aaatacaagc ggttaaattc ggtaaaaaat ttacacaatc cgaccgcttg taaaacctag
     3841 tctgcaatat aaccaacatc gtccaacgtt ggattatttg ctccttgttt agccagctca
     3901 tcgcaaatct cattttcacg atgtcctgaa tgccctttca cccaactcca ttcaatttgg
     3961 tggcgttgaa tttcttggtc taaagcaacc cataaatctt tatttttaac tggcttttta
     4021 tcgctggttt tccagtcatt tttcttccag ttaaaaatcc atttttggat cccgtttttc
     4081 atatattggc tgtcactatt gagttgcacc ttacaaggct cttttagcat tgctaaagcc
     4141 tcaatcaccg cccgcaattc cattcggtta ttggtggttt gaaaatagcc tttgctcacg
     4201 gttttttcgt acccgttata acgcagcaaa ataccaatcc cccctttgcc ggggttgcct
     4261 aaacaagaac cgtctgtaaa aatttcaact aatttcatca tcacgctttg aaaataaaag
     4321 ccccaaagca ttaactttgg ggcagctttt aggctaaatt ctaaaagtta tcagaatgga
     4381 gatcaactga ttattttttt ccaagttgtg gaatcacgct attattagcc gccaataaac
     4441 ctgcatcggt ataaactgct aatttaccac gggtatcgac aatatctaag ttacgcattg
     4501 ttaattgacc gatacgatct gccggcgtaa acggtgcatc ttctactttt tccatactta
     4561 aacgttctgc ttcgtaggtt aagtttggcg attcagtgtt aagaatagtg aaatcgttac
     4621 cacggcgtaa ctcaagcgtg accgtaccgg taatcgcttt tgccacccaa cgttgagccg
     4681 tttcacgcaa cattaaagct tgcgggtcaa accagcggcc ttgatacaat aaacgcccta
     4741 aacgtaatcc gttaatgcga tattgctcaa tcgtatcttc gttgtgaata ccggtaacta
     4801 aacgctcgta agcgatgtgt aataatgcca ttcccggtgc ttcataaata ccacgagatt
     4861 tcgcttcgat aatacggttt tcgatttggt ctgacattcc caaaccatgt ctgccaccaa
     4921 tgcggttagc ctccataatt aattcaacgg catcatcaat acgtttaccg tttaacgcca
     4981 ccggcacacc ttcttcaaag gtaacagaaa cagtttccgc tttaatttca actgattcgt
     5041 cccagaatgc cacgcccata atcggtttta cgatcttcat accggtgctt aattcttcta
     5101 aatctttcgc ttcgtgggtt gcacctaaca tattcgagtc tgtagagtag gctttttcca
     5161 ccgacatttt atagtcaaaa ccgttttcga ttaagaattt agacatttcc aagcgaccgc
     5221 ctaattcatc aatgaatagt tggtctaacc acggcttata aatttttaat tttggattgg
     5281 ttaataaacc gtaacgatag aaacgctcaa tatcgttacc tttaaaggtt gaaccatcgc
     5341 cccagatatt cacatcatct tctttcattg ccgcaaccaa cattgtgccg gttaccgcac
     5401 gaccaagtgg cgtggtgttg aaatacggaa taccgccggt tgaaatatgg aaagcaccgc
     5461 attgaattgc ggcaatgcct tcgtgagcta actgttggcg acagtcgatt aatcgtgcat
     5521 tttcagcccc atattccatc gcttttttcg gaattgcgtt gtaatcatct tcatccggct
     5581 gacctaaatt tgcggtgtac gcataaggca ctgccccttt tttacgcatc cataacagtg
     5641 ctgctgaggt atctaaaccg cctgaaaagg cgatacccac tttctgccct aaaggtaaag
     5701 cttctaaaat tgttgctgac atacgaattc ctataaattt tgaaagtaaa aatattcaaa
     5761 cgcaaacgct tacgttcaag caaaatattc tagctgaaaa gtagtaaaaa ttcatctaaa
     5821 atttagcccg atgatttgca aattttttgt gaaatttgac cgcttataat ttactgaaaa
     5881 cctaaaagca atagagaaaa tcagaatgaa aaaactactc gtaatccgta atgataaaat
     5941 tggggacttt atggtctgct ttccggcatt tgctatgttg aagcaatcaa tgccgaatgt
     6001 ggaaattacc gctcttgtgc caagttatac tgctccactt gccgagcttt gcccttctat
     6061 tgataagatt attattgata cggcgaataa aaaagataaa aatgaattta accgtatttt
     6121 acaagcggtt aaaaatgagc aatttgatgc ggttatttgt tttgtttcag actggcataa
     6181 tgccaaactc acctggcaaa gtgggattaa ataccgcctc gctccagcaa ctaaattatt
     6241 ccaattttta tataaccacc gtttaaccca acgccgttct caatcagcaa aagcggaatc
     6301 cgaatataat ttggatttag cccgtgcttt tttaagcaaa cacaatatac cgattgttga
     6361 acctcacacg ccatacttaa gtgttgaaca gcaaaccgtc caggcacaaa aagcaaaatt
     6421 atgcgaacaa ctcaatatta ccaccgttaa aaagtgggta tttgtgcata gttcaaccgg
     6481 tggttcggca aataatttat ctatagaaca gtatgcagag ttaattcaag gtattttggc
     6541 agaatttgat tgctatgtgg tactcaccgc agggaaaggc gaaagcgaaa aagctcagca
     6601 gttagctcaa caaatcaatc acaacaacgt ggtggtttat gataaaaatg aggggttaca
     6661 agattttaca cattcgcttg cgtgtgcaga tttgtttatt gccggctcaa ccggtccgtt
     6721 acatattagt ggggcgttaa atattccaac tattggcttc tacccgagcc gactttctgc
     6781 cattccacgc cgttggaaac cgattaatga tgcgaataaa cacattgcat tttgcccacc
     6841 gtctgataaa gcctcacaaa tgaatttaac tttaatttct atttctgagg cattaaaaac
     6901 gatagtaccg tttattcgga gaatgtggaa tactccctct ctctgataaa attgctaaac
     6961 gcaattttat ctatctctct cccactagag ggagagagta ctgaaaagca tcatttccga
     7021 gtacgattcc acaaatccgc atattttacg aaagtagaat gtgccgaaag aactgccaat
     7081 aaaaatcctt gcttgccatc taaaaaaccg gcttttaata gatacatttt cacaaaacag
     7141 ccgatggcgt gagtcacgcc atcaaataag ctggctgttt tgcctgcatt tgcccgttgg
     7201 attgcccacg ctttagcgta acttgccgat tttaccaaat agtggtgaat atctttatag
     7261 gtgaaatgca gcaaatcgcc ttgtagtttc tggatttttg cctcttttgg gtaatgtact
     7321 ttttcgtgaa ccaattcatc accatatttg gcaaattccg tgcgatacaa tctcaccaca
     7381 caatccggat accaacccga gtggcgaatt tgtttgccaa atacttcact taagcggctg
     7441 attttgtaaa cggtattttt ttcatcattt ttgaccgctt gtaaaataga ttgcttcagt
     7501 tcggctgtca ctcgctcatc tgcatcaagc caaagtacat aatcactggt cacatattgt
     7561 tgagctaact gacgctgttt accaaagcct tgccaatcgg tgttagtgta gaaccttgcg
     7621 ttgtattcag cagcaatttg tggtgtttca tcagtactgc cactatctaa aatcacaatt
     7681 tcatctaccc aatcttttac tgtgtcaagg caagcccgta aatcttgagc ttcatttttg
     7741 acaatcatcg caacacttaa tgtcggcata gtttatcctt ctctcttttt gtcgtgtgtt
     7801 tttctgctat tcgttgcatt ttactttaaa aatagtggtt tttgtgctat tttctacgcc
     7861 aaattttatc aacaaaacaa attaacggaa aaagaatgtc aattacatta acaaccaaac
     7921 aaaaacaatt cctaaaaggc ttggctcatc atttaagccc agtggtgatg ctggggggta
     7981 acggcttaac agaaggcgtg ttagctgaaa tcgataacgc cttagaccac cacgaactga
     8041 ttaaagttaa agttgccggg gctgatcgtg aaaccaaaca attaattatt gatgccattc
     8101 tgcgtgaaac taaagcggct gaagtacaaa ccattgggca cattttagtg ctttaccgcc
     8161 aaagcgaaga gaaaaaaatc agcttgccaa aagcaactaa agcgatctaa ttgcaaaaat
     8221 atgtttaaat tcaaccgctt gtattcaagc ggttttttat aggactttct atgtttaaaa
     8281 aattctctct ttataccctt gcaatgttgc tcattcctgt ggtgacttgg ctggcaaatt
     8341 ggcactggtc tctggcaatt caatacgctg aatttgattt cgactacttt ttatatttaa
     8401 tcaccgaaac cggctccgta ccttatgcca tcatcacctg tgtggttttc gccttattac
     8461 tggcgagggt ggttcgcaaa cgctactctt taatttttgt gattaccgtg tgtgtcgctt
     8521 caatggtagc cactcaagca atcaaaacag cgatgaaatc aacctttcaa gagcctcgtc
     8581 catttgtcac tgctcttttc caagataaaa cagacgattt ttatcaatta acacgcgagc
     8641 aacgtgggct tgccgtaatg gaaaaaatcg gtgcaaatca aaattttgtt accgctcacc
     8701 aagccaaaga agtgggctat tctttcccat caggccacac tatttttgcg gtttcttggc
     8761 tattattggt ggtaggtttc tgccgtaata tgcgaggaca agcggtcgtt ttggctcatt
     8821 tttttgcaat cagttgggcg gcgttaatgc tgattagccg cttgcgttta ggtatgcact
     8881 acccgattga tttattcacc agtagtttaa ttgcgtttct aatcaattta gccttatttg
     8941 tgtggtttgt gccttggttg gaaagtaaaa aatggtttgc ta
//
LOCUS       DANLXQ010000103         8889 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.103, whole genome shotgun sequence.
ACCESSION   DANLXQ010000103 DANLXQ010000000
VERSION     DANLXQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.103"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            111..1301
                     /gene="malE"
                     /locus_tag="PXD94_001826"
     CDS             111..1301
                     /gene="malE"
                     /locus_tag="PXD94_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL6286664.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAPKSWEEIIELD
                     KKLKKEGKNAIMWNLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQF
                     VVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLNG
                     QASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQEK
                     LAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSRI
                     QKQQ"
     gene            1420..2958
                     /gene="malF"
                     /locus_tag="PXD94_001827"
     CDS             1420..2958
                     /gene="malF"
                     /locus_tag="PXD94_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL6286665.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            3011..3901
                     /gene="malG"
                     /locus_tag="PXD94_001828"
     CDS             3011..3901
                     /gene="malG"
                     /locus_tag="PXD94_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL6286666.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTPPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            3976..6033
                     /locus_tag="PXD94_001829"
     CDS             3976..6033
                     /locus_tag="PXD94_001829"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL6286667.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDDLEKHFFQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTPTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(6147..8873)
                     /gene="malT"
                     /locus_tag="PXD94_001830"
     CDS             complement(6147..8873)
                     /gene="malT"
                     /locus_tag="PXD94_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL6286668.1"
                     /translation="MLIPSKLVCSFRLQNSIPRKRLIQELDKSCSYPVVLINAPAGYG
                     KTTLVSQWIENKKNIGWYALDEGDNNSDRFATYFSAALHSAINEEIDVLLEENRKANL
                     LALFNQLLIKAAAFSEHFYLAIDDYHIIENDEIHEALKYWIKHQPSNMTLILISRSIP
                     PLSIASLRVQEQLLEIDINQLMFDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTA
                     LQLISLFAKNKSHTLQVPLQDIAKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCS
                     VLHSMNESLVQAVTGEQNSRTKLESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASF
                     LNLCCQNELYAELPQLHQKAANAWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVF
                     HQGELKLLEESLNCLDYAHLTEHTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENK
                     IELSKTANAEFNVLRAQVAINSGDENTALQLASEALNDLSENAYYAHIVATSIIGEAH
                     HCHGNLAEALAMLQKAERMARQHHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASE
                     FVKENHLQKVPMYEFLLRSKGKILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLT
                     KISLARGNLDNTARLLGEVEQLEHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLI
                     QNPSPVSDKNHFTQVQWRNIARSRILLEEYHEAKAILDNLIETAEKFSLISDLNRALI
                     VRNRLYFLQGEKELAQQDLIQALKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDEL
                     VLHKAQFILRNINQFYRQKFAHFDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIY
                     SGYSNEQISDELQVAATTIKTHIRNLYQKIGVTNRNEAISYTKELLMLMGYH"
BASE COUNT         2655 a         1792 c         1756 g         2686 t
ORIGIN      
        1 cgtagagcaa agggtgatct caaatagatt gtatagccca taatttgcca taatttaata
       61 aatgggttgt ttcatatccg tttcttttat ttaacttcaa ggagtttttt atgaaaaaca
      121 aatgtgtcaa attcacctta gctactcttg ctaccttagt tctttcagga acagtaatgg
      181 caaaaatgac ggaaggtaaa ctcgttatct ggattaacgg tgataaaggg tacaatgggc
      241 ttgccgaagt cggtaagaaa tttgaaaaag ataccggtgt tcaagtttta gttgaacacc
      301 cggatagact tgaagaaaaa tttgcacagg ttgcctcaac tggtgacggt ccggatatta
      361 tgttctgggc tcacgaccgc tttggtggtt atgcacaatc cggtttattg gctgaagtga
      421 atgtaagcaa agaatttaaa gataaatttg tcgattttgc ttgggatgct gaaacttaca
      481 acggcaaaat tatcggttat ccggttgcga ttgaagcgat ttcattaatt tataataaag
      541 atttagtgaa agaagcacca aaatcttggg aagaaattat tgagttagat aaaaaactca
      601 aaaaagaggg caaaaatgcc attatgtgga acttatccga gccctatttc acgtggccga
      661 ttgcggcttc aaacggagct tatgcattca aatttgctaa cggcaaatac gatccaaaag
      721 atattggtgt gaacaatgaa ggggcagtaa aagcactaca atttgtagtc gatatggtga
      781 aaaataaaca tattagtgcg gatatggact atgccgttgc cgaagcctcc tttaataaag
      841 gacaaagtgc cttaaccatc aatggtcctt ggtcttgggg caatattgat aaaagcggta
      901 ttaaatatgg tgtaacttta ctcccaacat taaacggtca agcttctaaa ccattcgtag
      961 gggtgttaag tgccggtgta aacagctcaa gcccaaataa agatttagcc aaagaattct
     1021 tagagaatta tttactcacc gacgacggtt tagatgcagt caataaagat aaacctttag
     1081 gtgcggtggc gttaaaatct taccaagaga aattagcggc tgatcctcgt attgctgcaa
     1141 cgatggcaaa tgcgaaaaac ggtgaaatta tgccaaatat tccgcaaatg tcttctttct
     1201 ggtatgccga aaaatctgct atcaataatg cagtgaccgg tcgtcaatcg gtgaaagaag
     1261 cattagatga tgcacattca cgcattcaaa aacaacaata attcttcatc attctgagtg
     1321 ggttattatg acccactcta tttttagatt caccctaaat acaataaaag tacagtaaaa
     1381 gtaccgtaac aaaatgcagt aaaaattcga ggtgctgtta tgctaactca aactcaatcc
     1441 aaatccactc attggctcaa atatctctta gccggattag tcttgctcct tgacttctat
     1501 cttgttgtct taatgtactc acaaggtgaa tatctttttg ctattttaac gctgattatt
     1561 ctaacttccg gtgtctatat ttttaccaat aaaaatacct atgcttggcg gtatgtttac
     1621 cctggcatta ccggaatggc aatttttatt ttgtttccgc tggttgcgac tattgcaatt
     1681 gcgtttacca actatagtgg ttctaatcaa ttatcctttg aacgagcggt atccgtctta
     1741 actgaacaac gctattttgc cggagataaa tatcaattca ccctttatcc gcaagcagat
     1801 aataaatatc aaattgcatt aaccaatccg acgacagagc aaacttttgt gtcagagccg
     1861 atttcattgg ctaccggaac gaatgtggtt gtaaccgagc aaaacagacc aactggcgaa
     1921 gtcgctcccc ttaaagtgat tacgcaaaat cgggccgcac ttcaatcaat gaaagtggtt
     1981 ttaccgaata ataatgaact gacaatgagc tcattacgtc aattctccga gcaaaaacca
     2041 cgctatcaat ttgatgaaga aaataaaata ttactcaata atgagactgg caaacgttat
     2101 caagcaaatg acgaaaccgg tttcttccaa tcgattgatg aaaacggtca ttggcaaagc
     2161 gaaacacttg aaccaggtta tactgtcaca tcaggcttta ataacttcat taaaattttt
     2221 accgatgaag gtattcaaaa accttttgtc caaatattta tttggacggt gatcttctct
     2281 ttattaaccg ttgtctttac cgttattctc ggtatggttc ttgcctgttt ggtacaatgg
     2341 gaggcattaa aaggtaaagc agtctatcgt gtcttattga ttttacctta tgccgtacca
     2401 tcatttattt cgattctgat ttttaaagga ctatttaacc aaagttttgg tgagatcaat
     2461 atgattttaa accaactatt cggcatcagc cctgaatggt ttaacgatcc gctccttgcc
     2521 aaagtgatga tcttaattgt caatacgtgg ttaggttatc cttatatgat gattttatgt
     2581 atggggttac tcaaagctat tccggctgat ttatatgaag cctccgcaat ggacggtgct
     2641 tccacttggc aaaacttcag taaaatcact ttcccattac tgttaaaacc acttactccg
     2701 ttaatgattg cttcgtttgc atttaacttt aataactttg tattaattca actattaaca
     2761 aacggtcgcc cggatatgat tggaacaacc actccagccg gttataccga tttattagtc
     2821 agctacacct atcgtattgc ctttgaagga agtggtacgc aagatttcgg cttagcagcc
     2881 gccatcgcaa ccattatttt cttattagtc ggtggcttgg cactcttaaa tatcaaagca
     2941 accaaaatgg aattataaaa gcgggaggga tttaacaatc ccgaccccaa actaacttta
     3001 ggagttatat atggcaattg ttcaatctaa atctgtgcgt tatcgggtat gggcaaccca
     3061 tttcatctta atctgtttcc ttgccttaat tattttccct ttattaatgg tgattggcat
     3121 ttcgttacga ccgggaaatc tggctatcgg tgatattatt ccaagccaaa tttcgtggga
     3181 acattggcaa gccgctcttg gttttgatgt tacacacgcc gatggtacag tcacaccgcc
     3241 gccattcccg gtgctacgct ggttatggaa ctccatcaaa gtggcaacaa ttacctctat
     3301 cggcattgta accttatcta cgacctgtgc ttatgcattt gcccgaatga aatttaaagg
     3361 caaaaaaacc attctacaag gtatgctgat tttccaaatg ttcccggcag tgctttcctt
     3421 agttgcctta tatgcgttgt ttgatcgtct tgggcagtat gtaccgtttc ttggtttaaa
     3481 cacacacggt ggtgttattt ttgcctactt aggcggaatt gccttacacg tttggacaat
     3541 caaaggctac tttgaaacga ttgatggttc attggaagaa gccgcttccc ttgatggtgc
     3601 aaccccgtgg caagcgttcc gattaatctt attaccgctt tcagtgccga ttttggcggt
     3661 agttttcatt ctttcattta ttgccgccat taccgaagta ccggttgcct cactcctatt
     3721 gcgtgatgta aacagctaca ccctagccgt tggtatgcaa caatacctct atccgcaaaa
     3781 ctatttatgg ggtgacttcg cagcagcggc ggtcttatcc gcaattccga ttacgctggt
     3841 cttcttattg gcacaacgct ggttaatcgg tggtttaact gccggcggcg tgaaaggcta
     3901 gtttgcaaaa aattctctaa aaacgaccgc ttgtcgagct gatactgcgg tcgtcaatct
     3961 ttattagggc tgtctatgaa aaatgcgtta ccttatctct gcttattcta tacctcaagc
     4021 ctttttgccc aatcttggca acattcccat ttttctgatt ttaacgatga tctcgaaaaa
     4081 catttttttc aatcccaagt tcaattggaa aaagggagct atccgcttag gttcaaattt
     4141 ggcgaacaat gttatcaacc ccaacaagcg ataaaattaa atcaatcggt tgcgttagtc
     4201 ccttgtataa atgaagctcc acaactgcgg ttgtttcgtc aaggcaacta tctggcacaa
     4261 attgatctgc gaagcggcac tccgacaccc acaatcagcg tagaacagca gctacagcac
     4321 aatgaagcga tgtttcaaag ttgcccgaat tgggataaaa aaccgattga aattgatgtt
     4381 tcttccactt ttgccgaagg cgaatcggtt agcgattttt attcaggcaa caccgctatc
     4441 gttaaaaacg gcaaagtcac cttaatgccc aatgaaaacg caagcggtct gattttgctg
     4501 gaaaaaagca ataccgaaaa tacggcaacg tttgattgga aaaatgccac cgtctatttt
     4561 gtgctgaccg accgctttta taacggcgat ccgaccaatg accacagcta ccaccgccaa
     4621 aaagacggaa tgcaagaaat cggcaccttt cacggtggtg atttaaaagg attaaccgaa
     4681 aagctcgatt acctacaaca acttggggta aacgtattgt ggattagctc tccgttagaa
     4741 caaatgcacg gctgggttgg cggcggtgat aagggggatt ttccgcatta tggctatcac
     4801 ggttactatc atttagattg gacgaaatta gatgccaata tgggtactga agacgattta
     4861 agtcgctttg ttcaacaagc ccaccagcgt ggtattcggg tgctatttga tgtggtgatg
     4921 aaccataccg gctacgcgac gcttgccgat atgcaggagt ttaactttgg cggattttac
     4981 ttaacaccgg acgaaattag ccgcacatta ggcgaaaaat ggacacactg gaagccgaaa
     5041 tcaggacaaa attggcacag tttcaatgat ttcattcgct ttaacgataa tcaggcttgg
     5101 caaaattggt ggggaaaaga atgggttaga gctgatattg ccgattacga cagccctaaa
     5161 tttgatgatt taaaaatgtc gctcgctgcc cttcctgatc taaaaaccga gagcgaacaa
     5221 gcggtcaaat tacctgaatt ttttgcaaat aaaaatacca atgccaaaac attaccaaac
     5281 gccaaagtgc gtgattattt aattggttgg ttatccgatt gggtcagaaa atatggcatt
     5341 gacggttttc gggttgatac cgccaaacac gttgaaaaat ccacgtggct ggtattaaaa
     5401 caatcggcac aacaagcctt aaacgaatgg caaaaagcca atccgaacgc cggttttaac
     5461 gatcgctttt ggatgacagg agaagcgtgg ggacacggcg tgttcaaaag tgactattat
     5521 caaaacggtt ttgatgcaat gatcaatttc gattttcaag atcaggctaa acaagggtta
     5581 aattgctttg cccatattga gccgatttat gccgaaatga atcgcaaatt aagcgacttc
     5641 aatgtgttga gctatttatc ttcacacgat actcgattat ttttccattc ggatagtgaa
     5701 caaaatatcg aaaaacacaa aaccgccgca aatttgctgt tgctttcacc cggtgcagtg
     5761 caaatctatt atggtgatga aagtgggcgg acatttggtg cgaccggttc agacctgata
     5821 caaggcaccc ggtcggatat gaattggcaa gagttgcagc aagatcctca gaaacaggca
     5881 ttgcatcaac attggcaaaa attgacacaa ttccgccaac gccatccggc tattggcgca
     5941 ggtgtccatc aactcatcaa aaatgatagc tactttgcat ttaagcgtgt gttgaatgaa
     6001 gataaagtga tggtggtttg ggccggaaat taaatctact ttagcttcaa gttttctctc
     6061 ttctggggaa aacttgaagc cggttcgggg aaacaagttg caaaaaatta ctaaaaatag
     6121 accgcttgtt ccccatgagc ttgtgatcaa tgatacccca tcaacatcag cagttctttc
     6181 gtatagctga ttgcttcgtt acgattcgtc acaccgattt tctgataaag atttcggata
     6241 tgggttttaa tcgtggttgc agccacttgc aattcatctg aaatttgttc attgctatat
     6301 ccggagtaaa tcaaacccaa tacttgccat tcccgctgag tgagcggact aatttttaag
     6361 agttccggca ctttcggatt tttcaataat tgattcacaa agttttcatc aaaatgggca
     6421 aatttttggc gataaaattg attgatgttg cgtaaaataa actgtgcttt gtgcaatacc
     6481 agttcatcta gcacattaag ttgcaataaa tggcggatct gctgtgccat tacatcccct
     6541 tcaatcacaa acgcactgat aaaattcgtt tggcgggtga gttttagtgc ctgaattaaa
     6601 tcttgctgag ccaattcttt ctcgccttgt aaaaaataaa gccggttacg aacaattaat
     6661 gcgcgattta aatcgctaat cagagaaaat ttttcagccg tttcaattaa attatctaaa
     6721 atggctttcg cctcgtgata ttcctcaagc aagattcgag accgtgcaat atttcgccat
     6781 tgcacttggg taaaatgatt tttatctgaa accggtgagg ggttttgaat taaccagttc
     6841 ctcaccgcag aaacgtcatt ggtcatctgc caataaaata tacgtacctg atcggcgttg
     6901 gcaatccaat cgtggtgata agaatgagaa tgttctaatt gttcgacctc gcctaataat
     6961 cgggcagtgt tatccaaatt gcctcgagcc aacgagatct tcgtgagtaa agttaagcat
     7021 tgtaacttat cttcaaattt agataaggca ttcattccgg caactgccat tgattcagct
     7081 ttatctaagt tataccattc ccataaaatc ttgcctttag aacgtagcaa aaattcatac
     7141 atcggcactt tttgtagatg gttttctttc acaaactcgc ttgctttatc taacatttca
     7201 taggctgctt gtgaaaaccc ttgggcaagc aaaatttcag attgttgaag ctgtgaccac
     7261 aaaatattgt ggtaagtatg atgctgacgt gccatacgct ctgctttttg caacattgct
     7321 aatgcttctg ccagattgcc gtggcaatga tgagcttcgc caataataga cgttgcaaca
     7381 atatgagcat aataggcatt ttctgaaaga tcgtttaacg cttcagacgc taattgaagt
     7441 gcggtattct catcaccgga attgatagca acttgtgccc gtagcacatt aaattccgca
     7501 ttggcagttt tgctcaattc aattttattt tcagttaatg cacgagaaaa atccgctaaa
     7561 ataccaccta cttcaatatg gcggtgctgg ctttgtacca accaggcttt gagtaacacc
     7621 aaattagtat gttcggttaa atgggcgtaa tctaagcaat ttaagctctc ttccaataat
     7681 ttgagttctc cttgatgaaa caccgtccaa gcgtgtttgc tcaaaatatt gagtagtaat
     7741 ccggtatccg ataactgcat tgcgtgatgt aaggcttctg taacatagcc taattgtaac
     7801 cacgcattcg ccgccttttg atgcagttgc ggtaattcag catataactc attttggcaa
     7861 cataaattta aaaaggaagc aaaaagcgga tggaatttcc accagctatc atcactactc
     7921 tgccacttac tgtttgccat ttgttgtaaa aacaaacctt gcttttcgag agactctaat
     7981 ttggtgcgac tgttttgctc ccctgtaaca gcctgcacta aagactcatt cattgaatgc
     8041 aaaaccgaac aacgcaaaat aaacaaacga gtctcttcat cgactttatt aagcacttca
     8101 tcggctaaat attcgttaat atgaaaatgg tttgatttgg ctaaacgttt ggcaatatct
     8161 tgcagtggta cttgtagcgt gtgtgattta ttttttgcaa aaaggctaat tagctgaagg
     8221 gctgtgggcc acccttcaac ttcgttacac agtgcgataa catcttgctg atttaactcc
     8281 gaacctaatc ttgcttgaaa aaaagcggta ctttcttggt gatcaaacat taactggtta
     8341 atatcaattt ccaataattg ttcttgcact cttaaactgg caatacttaa cgggggaatt
     8401 gatcgagaaa tcaagataag cgtcatatta gacggttgat gtttaatcca atattttaat
     8461 gcttcgtgaa tttcatcatt ttcaataata tgataatcat caatagcaag ataaaaatgc
     8521 tctgaaaatg ccgcggcttt aatcaataat tgattaaata aagctaataa attagcttta
     8581 cgattttcct ctaacaatac gtctatttct tcattgattg ctgaatgcaa tgccgcacta
     8641 aaataagtgg caaaacgatc tgaattatta tcgccttcat ctaacgcata ccagccgata
     8701 ttctttttat tttcaatcca ttgggaaacc agagtggttt tgccataacc tgccggtgca
     8761 tttatcaata cgaccggata actgcaagat ttgtctaatt cttgaattaa acgttttcga
     8821 ggaatgctgt tttgtaatcg aaaagaacaa acaagttttg atggtattaa cataataaat
     8881 tcaattttc
//
LOCUS       DANLXQ010000104         8801 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.104, whole genome shotgun sequence.
ACCESSION   DANLXQ010000104 DANLXQ010000000
VERSION     DANLXQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.104"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            13..1326
                     /gene="pilQ"
                     /locus_tag="PXD94_001831"
     CDS             13..1326
                     /gene="pilQ"
                     /locus_tag="PXD94_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL6286669.1"
                     /translation="MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNM
                     VLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPI
                     MNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
                     SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGH
                     YKFGGRLEGNGLNNVTNNLNVNFPITGGASAVLQVSSINSRVLDLELSALEQENSVEI
                     IASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQIL
                     LDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPI
                     LGSIPFIKRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK"
     gene            1407..1820
                     /gene="nusB"
                     /locus_tag="PXD94_001832"
     CDS             1407..1820
                     /gene="nusB"
                     /locus_tag="PXD94_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL6286670.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            1883..2863
                     /gene="thiL"
                     /locus_tag="PXD94_001833"
     CDS             1883..2863
                     /gene="thiL"
                     /locus_tag="PXD94_001833"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL6286671.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            2868..3341
                     /locus_tag="PXD94_001834"
     CDS             2868..3341
                     /locus_tag="PXD94_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL6286672.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHLLYYFI"
     gene            3348..3971
                     /locus_tag="PXD94_001835"
     CDS             3348..3971
                     /locus_tag="PXD94_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL6286673.1"
                     /translation="MLTILFIHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIG
                     IIFWAAFAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQL
                     TPLNPWQEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYF
                     CVVALLFSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            4083..4688
                     /gene="ruvA"
                     /locus_tag="PXD94_001836"
     CDS             4083..4688
                     /gene="ruvA"
                     /locus_tag="PXD94_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL6286674.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKGMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            4825..5829
                     /gene="ruvB"
                     /locus_tag="PXD94_001837"
     CDS             4825..5829
                     /gene="ruvB"
                     /locus_tag="PXD94_001837"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL6286675.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVHDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(5928..6611)
                     /gene="artM"
                     /locus_tag="PXD94_001838"
     CDS             complement(5928..6611)
                     /gene="artM"
                     /locus_tag="PXD94_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL6286676.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(6611..7282)
                     /gene="artQ"
                     /locus_tag="PXD94_001839"
     CDS             complement(6611..7282)
                     /gene="artQ"
                     /locus_tag="PXD94_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL6286677.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            complement(7282..8007)
                     /locus_tag="PXD94_001840"
     CDS             complement(7282..8007)
                     /locus_tag="PXD94_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL6286678.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEARQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            complement(8033..8767)
                     /gene="artP"
                     /locus_tag="PXD94_001841"
     CDS             complement(8033..8767)
                     /gene="artP"
                     /locus_tag="PXD94_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL6286679.1"
                     /translation="MAIRIQNVNFFYGSNQALFDINLEIEKGDTVVLLGPSGAGKSTL
                     IRTLNLMEVPQSGTLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQN
                     LIEAPMKVLGLSKEEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQP
                     QVLLFDEPTAALDPEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGT
                     IIEQGDASCFESPKTEQFAQYLSHSE"
BASE COUNT         2648 a         1874 c         1704 g         2575 t
ORIGIN      
        1 atagaggttg atatgtggcg aatttttata tatttcttat gcggtttttc attagcctat
       61 gcgaatacca tttccatagt tttaaaaaat gctccaactt cgcttatttt tacttattta
      121 gcggaagaaa ccggtaaaaa tatggtgctt gatgataata ttgaaaccaa atcaacacta
      181 cgattagaaa ataaatcggt tgacgagatt tttaaaacgc ttggcaaagt caataaatta
      241 tccttaacgc aagaagatga tattgtttat attcacaaaa aagaggaaaa agcggctgac
      301 ttaacgcctc tgccaataat gaatttgcaa aataatgggc aaaatacacc gcttgtcacc
      361 gcccctaagt taatcacaaa aaccttgaaa ctacattatg caaaggcttc agaggtgatt
      421 gagtcgttaa ccaaaggcag cggaacgttt ttatcggaaa acggttatat tcactttgat
      481 gaaagaagca atagcctgat tgtgaaagac agtgcaaaat cgcttaaaaa tatcgagaat
      541 ttagtaaaac agctcgacca acccaccgag caaattgcga ttgaggctag aattgtcact
      601 atcagtagcg agcatttgca agagcttggc gttcgctggg gaattttttc tcgtggggct
      661 gggcactata aatttggcgg acggctggaa ggcaatggac tcaacaatgt caccaataac
      721 ttaaatgtaa attttccgat tactggtgga gcatctgccg tattacaggt ttcgtccatt
      781 aatagcagag tgcttgattt agaattaagt gcattagaac aagaaaatag tgtggaaatt
      841 attgccagcc cacgcctgtt aaccaccaat aaaaagccgg caagtatcaa gcaaggcacg
      901 gaaattcctt atgtgatgta caacaccaaa tcggaagcca ccgatgtcga atttaaagaa
      961 gcggtattag ggcttgaagt cgttccacac ctttcaaccc aaaatcaaat tctactcgat
     1021 ctgatcgtta cccaaaattc gccaaattcc caatccggca gtagtggctt aattacgatt
     1081 gataaacaag agctaaatac gcaagtattt gccaaacacg gcgaaaccat tgtacttggt
     1141 ggtatttttc aacatttaac ccaaaaaggc gaagataaag tgccgatttt aggctcaatt
     1201 cccttcatca agcggttatt tagtcaaact cgagataaaa tcagcaaaag agagctagtc
     1261 atttttgtga cgccttatat ccttcaatct aatgaaaaga gcagcaaccg caaaaaatca
     1321 aaatagcaca tcataaacgc aagcaaaaat aggggaagtg tgatagaatc agccgtcatt
     1381 ttttgagtta aagatttaga ggttttatga aaatttcccc acgtcgccgt gcaagagaat
     1441 gtgcggtgca agccatttat tcttggtata tttcccaaaa cacggttgaa gaagtggaat
     1501 tggcgtttgt gaccgatcaa gatatgaaag gcgttgatat gccttatttc cgtaaacttt
     1561 tccgtggcgt tgtggataat gtagaagcca ttgatgaagc attacgccct tatttagatc
     1621 gtaaagagtc agatgttgat cctattgaac gttctatttt gcgtttatca acttacgaat
     1681 taaaatttga acacgatgtg cctttccgtg tagtcatcaa tgaagggatt gaagtcgcta
     1741 aagtgttcgg ctctgatgac agccacaaat acattaacgg cattttagat aaattagccc
     1801 cagcattagg cagaaaataa taagattccc caatttatac ataaattggg gattttgatt
     1861 ataacgctca aggagatttt ttatgggtga atttgatatt atcgaacgct attttaatgc
     1921 ttctcaacgc ccacctcgca aagatgtttt actttctatc ggcgatgact gtgcgctcac
     1981 atcactcaaa ccaaatcagc aacttgcgat cacaaccgat acccttgttt gcggcactca
     2041 ttttctaccg actatttcac ctgccgatct cgcttataaa agtgtggcgt ccaatttaag
     2101 cgatcttgcc gcaatgggtg cagaacctgc atggatctct ttagccttaa ccttacctga
     2161 aattaatcac gattggctag ctgaatttag tgaacatttc tttgagattc ttgatcacta
     2221 taatgttgat ttgattggag gggatactac ttgtggacca ctatctttga caatcacagc
     2281 tcaagggatt gtgccacaag atagaggctt attccgccat aatgctaaag taggcgattg
     2341 gatctatgtt tccggcacac tcggtgacag tgcaggtggt ttgcaacttc tgctaaatca
     2401 gcaaagcgag caagtgaatt ggaataaaga gcaaagctac ctgattcaac gccatttacg
     2461 cccaacacct cgagttttac ttggcttgac tatcgcctct attgctaatg cagcgattga
     2521 tatttcagat ggtctattgt ctgatttaag ccatatttta aagcgcagtg actgtggtgc
     2581 agtattaaac cttgagaatt tccccttgtc tgacgtctta cttagttgtt attctcgaac
     2641 agaggcagaa tattttgcct taaacggtgg cgaagattac gaactctgct ttaccgtgcc
     2701 aaatgacaac aaagccaaat tagacaaagc attagccaat atcggagtaa cttatacatg
     2761 tattgggcaa attcgttcgg ctaaagaagg tttaacccta ttacgtcata atcagcctgt
     2821 caccttgccc tcacaacgag gctacgatca ttttaaaaaa taacgcaatg aaaccaatta
     2881 atcttaaaaa tccgattcat ttttttgcct ttggctttgg ttcaggctta ttaaagcctg
     2941 ctcccggcac ctggggtagc ctcgccgggc taattgtagc aatagccttg tggtctatca
     3001 cacaatccgc cctgtttttc ggcttactca ccctagtttc ttttattgcc ggttgtgtta
     3061 tttgcaccaa agccagccaa gatctagaag tacacgatga cgggcgaatt gtttgggacg
     3121 aaatagttgc catctttttg atgttcacct gtttgcctga atacaatctt cttggctacc
     3181 tgctcgcctt tatcagcttt cgcatttttg atattttgaa gccttttcca atccgctatt
     3241 ttgatgaaaa attagaaagc ggtttaggca ttatgtttga tgatattctc gcagccattt
     3301 ttgccctgat ttcacttcat cttttatatt atttcattta aaaatttatg ctcactattt
     3361 tatttattca ttttgccggg cttatcagcc ccggccccga tttcttctat gtcgtcagac
     3421 aatccgccag ccactcagtt aaaagtgggg tacttgccgc aaccggaatc tcaatcggca
     3481 ttattttttg ggcagccttt gcgatttttg gtttagcgtg gctcagtaat agtattggct
     3541 cgctgtttca atatgccatt atgattatcg gtggctgtta cctggtttat attggctctc
     3601 aaatggtacg ggtaaccgaa aatatcattt ttgttgaaaa taaagctcaa ctcacgccgc
     3661 taaacccttg gcaggaaatt aaaaaaggct tattaatcaa tatctttaat gccaaagctg
     3721 gcgtttactt taccagcgtc atttcagcct atgtagataa tttaagcaat accctacaaa
     3781 tgttcgaact acttctactc tttgtggtaa gtacctttat ttatttctgt gtggtggcac
     3841 tactcttttc tcgccgaccg gtcagactgt tctatgccaa atatagtcgc tatattgaca
     3901 atatctcagg cgtaattttc atcttattcg gctcgatgct gatttatgaa ggattcagcc
     3961 actttatcta atcacaagcg gtctgatttc actaaaaatt tgcaaattcc gctatcaata
     4021 aatagtgtta ttttatacag tttctgctat gctactgcca attttaactc tgagagaaca
     4081 aaatgattgg acgattacac ggcaaaatta ttgaaaagca accgcctgaa attttattag
     4141 acgtgcaagg ggtaggctat gaactactgc tcccgatgac cagtttctac agcctgccac
     4201 aaattggcga agaggcgact attttcaccc atttagtggt gcgagaagat gctcacttgc
     4261 tattcggctt tgcccaaaaa caagatagaa ctctattccg tgaattaatt aaaaccaacg
     4321 gtgtcgggcc gaagctcgcg ttggcaattc tatcagcaat gtcggtgtcg caatttgcca
     4381 ccgcgattga aaatgaagag ctggcaaaat taaccaaaat tccgggaatc ggtcggaaaa
     4441 ccgcagaacg cttattggtg gaactcaaag gcaaatttaa aggaatggca cagagtgatt
     4501 tctttgtgga agtatcgcac gagcaaattg tggcaagtaa tacgccagac ccggcaaatg
     4561 aagcccttga tgccttaata gccctaggct acaaacctgc cgatgcggaa aaaatgatta
     4621 agaaagtgaa taaatccggt gcaacgagcg aacagcttat tcgtgaggcg ttaaaaaatt
     4681 cattatgata ttgatagtat agggcgaaag atatttcgcc cctacaatct catttttaaa
     4741 aatatatgtg taggggcaaa tcaatatttg ccctcatgag taatataggg taataccatt
     4801 ccactacaac aataatctaa atgtatgatt gaagcagata gaatcatcag tgcctcgcct
     4861 aagcgtgagg aagaagttat agaccgagca atccgcccga aactgcttgc cgactatgtc
     4921 gggcagcctt ctgtgcatga tcaaatggag atcttcatca aagccgctaa attgcgggaa
     4981 gaggcattag atcacttgct cattttcggc ccgcccggtt tgggaaaaac tacccttgcc
     5041 aacattgtgg caaacgaaat gggggtgaat attcgcacaa cttcagggcc ggtgctggaa
     5101 aaagcaggcg atttggcagc aatgctgacc aaccttgaac cttacgatgt gctgtttatt
     5161 gatgagattc accgcctttc tccagcgatt gaagaagtgc tttatcctgc aatggaagat
     5221 taccagctcg atattatgat tggggaagga cctgctgctc gttccatcaa gctcgatcta
     5281 ccgcctttta cccttgtagg ggcaaccacc cgtgccggct cactgacctc accgttacga
     5341 gatcgttttg ggattgtaca acgcttagaa ttttactcag tggacgatct tactctgatt
     5401 gtcaaacgca gcgccgattg cttaaatctc aatttgtcgg cagacggtgc ttatgaagtg
     5461 gctcgccgct cccgtggcac accccgtatt gccaaccgct tactccgccg agttcgggat
     5521 tacgccgatg tgcgtaataa tggcattatt acctcagaca ttgccaaaca agctctggca
     5581 atgctcgatg tggattcgga aggctttgat tttatggaca ttaaactgtt gcaagcgatt
     5641 gtagaacgct ttgatggcgg gccagttgga ttagacaatc ttgcggcagc tatcggcgaa
     5701 gaacgagata cgattgaaga tgtgctagag ccttatctga ttcagcaagg ctttctacaa
     5761 cgcacaccaa gaggcagaat tgctaccagc agaacctatg cccatttagg tataacccaa
     5821 atagattagt catatttcta aaggcattga tacataaata aaaagcggat agttagtact
     5881 atccgctttc atttgcaaaa aatcaataaa atttgaccgc ttatccgcta ggctttttca
     5941 gtctcaaaac gctcaaaagc taatactctg ctttctaatt ttctgagaac gattgtcata
     6001 atgccggtaa ttaccaagta aataacccct gccataccat aaattgataa tgcgtcatac
     6061 tctgtaccat aaagctggcg agcatagccc ataatatcca taatcgtaat ggttgaagca
     6121 agcgatgtgc ctttaaaaac tagcacaatc tcattactat aagaaggtaa agcacgtttt
     6181 aatgcgtaag gcactaaaat tttcagtgta tctaaacggc tcaaaccaag ggctgcgcaa
     6241 gtttcccatt gccccttcgg aatcgctttt accgcaccgt ggaacaacaa tgtcgaatag
     6301 gcagcactgt ttaacgctaa agccaacatt gcacagaacc aagcgtcgga taataatacc
     6361 caagccgcac tttctgtaat ccattcaaat tgccccgggc cactataaat caagaaaaat
     6421 tgaattaata aaggcgttcc ggtaaaaagc gtaataaatc cgtttaccaa ccactttaac
     6481 ggacgatttt ctaacgagag aataaacgta agaatgatcg ctaaaataaa ggcaatgatg
     6541 agcgccacaa aagtgagctg taggctggtg ccaataccct gtgaaatttc aactaaataa
     6601 tcccaccaca ttaagcaact cctcgctcaa aacgggtaaa tctaccttct aatactttaa
     6661 tcactcgctg gctaattaga gtaatcgcta aataaataag tgctgctatc ccataccaag
     6721 taaaaggttg atgagtatta gcattaatta actccgattg acgcattaaa tcatctaccc
     6781 caattaacga taccaatgcc gtatctttta acagcactaa ccactgattg cttaagcctg
     6841 gtaaagcgtg tcgccagact tgagggagaa taatgcgaac aaatacttgc gctcggctta
     6901 accctaacgc cgtaccagat tcccactgcc caactggaat agctttaatt gcaccacgta
     6961 aagattggga agcataagca gcaaaaataa tacccagcgc aatagcccca caccagaaag
     7021 catttaactc cgcaaactca tcggtgatca agaaaataac ttggataaga ccaaaataga
     7081 tcaaaaatac cactaaaatc tccggcaacc cacgcaataa tgtaattatt atactagttg
     7141 gtttacggat acatgcaatc ttactggttt caagtaccac aaaaaagatt gacaataaca
     7201 ttccaatcaa gaggcttgcc agcgcaatcc ctaaggtcat cagggttgcg ttgacaagta
     7261 agtttaaaat atcgctagac attatttccc agccatccaa gtattataaa tttgctcata
     7321 tttaccattt gttttgatgg tagctaaagc cgaatttaat ttttcaagca atgcagtgtt
     7381 agatttattt accgcaatac catatccatt accaaaaaag gctttatcga ctactttttc
     7441 acccacgaaa tctaactctt tttcggtttt tagccactct gccaatactg gtgtatcacc
     7501 aaataccata tcaacacgtc cattttttaa atctaacact gctgattgta ggctagcgta
     7561 aggttttaaa ttatattgtt tggcattttt gcttaagtat tgttgaaaag ttgtaccatt
     7621 ttgcacgccg acattcttag ctgtttccgg tgtaaatttc cccttcattg cgacataact
     7681 tactgagttt tctaaataag cattggtaaa ggcaacctgt ttttgacgcg cttcagtaat
     7741 atccatgcct gaaataattg catcaaaatt tttaaatttt aatcccgcaa ttaagctatc
     7801 gaatgattgc gttttaaatt cgcaagttgc ttttagttct tcacataatg cttttgcaat
     7861 atctacatca aagccaataa cttcaccttt ttcatttgtt gtttcaaaag gggcgtaaga
     7921 aggctccatc gcaaaagtga tagtttcttg tgcattggca gaaagcgttg caaatgcgat
     7981 agcggaagct aataataatt ttttcattgt tgtgtccttt tgttgttata gattactctg
     8041 agtgagataa atattgagca aattgctctg ttttcggtga ttcaaaacaa cttgcatcac
     8101 cttgttcaat aattgtacct ttttccatgt acaccacttt gcttgccacc ttacgagcaa
     8161 cgcctacttc gtgagtcaca atcacctgag taatgcctgt agattgcaat tctttaataa
     8221 tatcaactac ttgtgcagta atttccgggt caagtgctgc ggtaggttca tcaaataata
     8281 agacctgtgg ctgcatcatc agtgctctgg caatcgccac ccgttgttgc tgaccacctg
     8341 aaagatgcag tgggaaacgc tcggcaaact cgctcaaacg caaacgtgcc aaatgtcctt
     8401 ttgctctggc aaccgcttct tctttgctta acccaagcac tttcatcggg gcttcaatta
     8461 agttttgcat tacggttaaa tgcggccata aatgatactg ctggaacacc attcccacct
     8521 cacggcgtaa caaactcacc gctttgctgt cggttttagc agttaaatca aattgatgat
     8581 ttgcaatcgc caaagtgcct gattgcggta cttccattaa gtttaaggtt cgaattaggg
     8641 tacttttacc tgccccactc gggccaagca atacaacagt atcccctttt tcaatctcta
     8701 agttaatgtc aaataaggct tggtttgagc cataaaaaaa attgacattt tgaatacgaa
     8761 ttgccattcg ttaaaatcct actacattta aaataatgaa t
//
LOCUS       DANLXQ010000105         8770 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.105, whole genome shotgun sequence.
ACCESSION   DANLXQ010000105 DANLXQ010000000
VERSION     DANLXQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.105"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            16..1077
                     /locus_tag="PXD94_001842"
     CDS             16..1077
                     /locus_tag="PXD94_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6286680.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGIEKGEQELAKIHSQVNKNTISGYISERLVSPYRSINSATKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALPQIYMYVGNRLTINAKASATAFKTVVEEIGDEIYNIWKTNANLFC
                     IHPAGVGTPTNKSSFRKMFQYEVRDANTASVVSGALGIPISRLSSGKRDVLGKNVMVN
                     QSQLDAQVPNIQNLVQCIE"
     gene            1229..2881
                     /locus_tag="PXD94_001843"
     CDS             1229..2881
                     /locus_tag="PXD94_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL6286681.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAASGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKRPKAEINYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGAYVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDARGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
     gene            2934..3868
                     /locus_tag="PXD94_001844"
                     /pseudo
     CDS             2934..3868
                     /locus_tag="PXD94_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            complement(3890..5971)
                     /gene="recG"
                     /locus_tag="PXD94_001845"
     CDS             complement(3890..5971)
                     /gene="recG"
                     /locus_tag="PXD94_001845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL6286682.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQVCKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(5975..6403)
                     /locus_tag="PXD94_001846"
     CDS             complement(5975..6403)
                     /locus_tag="PXD94_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL6286683.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            complement(6403..6690)
                     /locus_tag="PXD94_001847"
     CDS             complement(6403..6690)
                     /locus_tag="PXD94_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL6286684.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
     gene            6854..8737
                     /gene="htpG"
                     /locus_tag="PXD94_001848"
     CDS             6854..8737
                     /gene="htpG"
                     /locus_tag="PXD94_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL6286685.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTDQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFADPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMRGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
BASE COUNT         2444 a         1854 c         2039 g         2433 t
ORIGIN      
        1 aaaagagggg gcattatggc tatttatgca atatggaata ataaaggtgg ggttggtaaa
       61 agttatttaa cttttcaaat cgcttgtgag tatgcaaaac aatacccagc aaaaaaagtt
      121 ctcgttgttg atttatgtcc tcaagcaaat tcatcatcta tgctattagg cggcattgaa
      181 aaaggtgagc aagagttagc aaaaattcat tctcaagtaa acaagaatac tatttcagga
      241 tatatttcag aaagattagt aagtccttac cgttctatta atagtgcaac gaaatatgta
      301 actcaagtta gcactataaa tgaatatgta ccaaaaaatg tttatcttgt tgttggtgat
      361 gaggaattag aaatccaatc atctggtatt ttacatgctt cccaagctgt tcatgttcct
      421 gattcttggc ggctagtgca tctttgggtg cgtgatttag tttctgatat acagaacgct
      481 tgggataatg atgataacgt tgtatttatt gattgcaatc caagtttcac tatttatact
      541 gaattagcgt tggctgcttc agatagattg atcatccctt tttccgctga tggttcatca
      601 aaacgagctg ttaagtctgt attatctcta gtatatgggg ttaaaaggca ctctggggat
      661 gtcagctctc atttttatac agaatctcaa aagagtaagt tagcattacc tcaaatttat
      721 atgtatgtag gtaatagatt aacgataaat gccaaagcat cagcaaccgc atttaaaaca
      781 gttgtagagg aaataggtga tgaaatctat aatatatgga aaactaatgc taatttgttc
      841 tgtattcacc cagcaggtgt tggtactcct acaaataaat caagcttcag aaaaatgttt
      901 cagtatgagg tgagagatgc aaatacagca tctgttgttt caggtgcttt aggtattcca
      961 attagtcgct tatcatcagg aaaaagagat gttttaggta aaaatgtaat ggtgaatcag
     1021 tcacaattag atgctcaagt tcctaacatt caaaatcttg ttcagtgtat agaataaaga
     1081 aaaatagcgt atattaagta cgctattttt tatttctgac aaatcttttt atttgatagc
     1141 tcgatcacaa aaaaagaaat atctcctttt aaataaaagg actttgggct aatctaaaca
     1201 gagtttttaa attacatagg agtaaaaaat gactattttc caagttgcac aaaattggtt
     1261 agcccaagac ccggatgcag agacccgtgc ggaattagag cagttaattc aagcggcaga
     1321 atcggacgag aaagcaaaag cagaattaac agcccgtttt gacggacgtt tacagtttgg
     1381 tacggctggt ttgcgtggtc gcttacaagc gggttcaatg ggtatgaacc gtgttttggt
     1441 ggcacaagct gccagcggtt tagctgggtt tattaaaggt tatgataaag agccgtcaat
     1501 tgttattggt tatgacggtc gtaaaaattc cgatgtgttt gcccgtgaca ccgctgaaat
     1561 tatggcagcg gcaggcatta aaacgtacct gcttcctcgc aaattgccaa caccagtgct
     1621 tgcctatgcg attcaatatt ttgataccac agccggtgtg atggtaactg ccagccacaa
     1681 cccaccggaa gataacggct ataaagttta tttaggtaaa gccaacggtg gcggacaaat
     1741 tgtttcgccg gcagataaag aaattgctgc tctgattgat aaagtagcag caggcgatat
     1801 tcgtgattta cctcgtagcc aagatttcac ggtgttagac gatgaagtgg taaatgctta
     1861 tattgagaaa accgcctcac tagccaaacg gccaaaagcc gaaataaact acgtttacac
     1921 tgcaatgcac ggtgtgggct atgaagtgtt aagcaaaaca ttagaaaaag ccggcttacc
     1981 gcaaccgtat ttagttagtg agcagattca gccggacggt tcgttcccaa cggttaattt
     2041 cccgaacccg gaagagaaag gggcgttaga tttagcgatt aaattggcga aagagaaaaa
     2101 tgccgaattt attatcgcca acgacccgga tgctgaccgt ttagcggtag ccgtgcctga
     2161 tgctcaaggc aactggaaac cactgcatgg caatgtgatc ggctgtttct taggctggta
     2221 tttagccaaa caattccacg cacaaggtaa acaaggcgtg ttggcttgct cgttagtatc
     2281 ctctccggcg ttagctgaaa ttgcgaaaaa atatggttta agctcggaag aaaccttaac
     2341 cggctttaaa tatatcggaa aagttgaaaa cctattattt ggctttgaag aagccttagg
     2401 ttatttggtt gacccagaca aagtgcgtga taaagacggc atttccgctg ccattatgtt
     2461 cttagattta gtgtgcagcc tcaaacaaga aggtaaaacc cttgcggatt acaccaccga
     2521 gtttgtacaa gagttcgggg cttatgtaag cggtcaaatt tcgatcagag tttgtgattt
     2581 agccgaaatc ggcaaattaa tgacggcgtt acgcaacaat cctccaagcg aaattggtgg
     2641 ctttaaagtg gctgagttta ttgaccatac caaaaccaca cgccaaaatg acattttagt
     2701 gtttgtgttg gaaaatggca gccgtttgat tgctcgccct tcaggtactg agccgaaaat
     2761 taaattctat ttagatgctc gtggcacaga cgctgaaaac gcagagcaag tgctggctca
     2821 gtttgacgaa agtgtgcgtt cgctgcttcg ccaagaagaa tacggcaaac aagattgcta
     2881 atggcattaa ttgaggggga gcttaggctc tccctttttt aaaggagaca ataatggctc
     2941 aaaaaattat tttagattgt gatccggggc atgacgatgc gattgcgatg atgctcgctt
     3001 gggggaatcc gaatattgat ttgttggcag tgactacggt ggtaggtaat caaaccttag
     3061 aaaaagtatc gcgtaatgcg ttggcggttg cggaaattgc tcatatttgc aacgttcctt
     3121 ttgccaaagg ttgtgccaga ccgctggtgc gtgaagtgga aaatgccccg gatattcacg
     3181 gtgattcggg aatggatggg cctgtactcc ctgaatctac ccgacaattt gagaaaaaac
     3241 acgccgctca attaattatt gatttgataa tgtcgcaccc ggagaaaagc attaccttag
     3301 tgccgaccgg tgggcaataa ccaatattgc tctagctgcc cgccttgagc cccgtattat
     3361 tgaaagagtg aaagaagtgg tgctgatggg cggaggttat cacaccggca actggagtgc
     3421 ggtggcagaa tttaatatta aaattgaccc ggaagctgct catattgtgt ttaatgccgg
     3481 ttggaaggtg acgatggtcg gcttagactt aactcaccaa gcacttgcga cccccgatgt
     3541 ggttgaacgc tttaaggcat taaacagcaa atcggggcag tttgtggtgg aattgctggc
     3601 gttcttcgcc aaaatgtata aacaggctca aggctttgat gctccaccgg ttcacgatgc
     3661 ctgtgcggtg gcgtatgtga ttgacccaag cctgattgaa gttcagcaag tgccggtcaa
     3721 tattgagcta acaggcacgc atacactggg tatgacggtg gcggatttcc gctacccgcc
     3781 aaaacagtgt aatacttggg tagcaaaaaa attagacaaa tcccgctttt gggatttggt
     3841 aattgaatcg gtgaaaaacc tgccgtaatc taaataaccc gcttgccggt taagcgttgg
     3901 tatattccgc cttgttgtct aaccaacgct taattaacgc ctctgcaagc ggttgatttt
     3961 cgctaataat ttgcttggcg taacgttgca ccagcggaat cattttgcgg tctcgcatta
     4021 aatccgccac cttaaattct gccattccgg tctgttttgt gccgagaatt tcgcctgtgc
     4081 ctcgaatctc caaatctttt tcggcaatgt agaaaccgtc ttggctatca cgcatcactt
     4141 gtaaccgctt gctggaaatt ttcccgagcg gcggtttgta cattaagacg caatgcgagg
     4201 cggttgcccc tcgcccaaca cgtccacgca actggtggag ttgagccagc ccgagtcgtt
     4261 cggaattttc gataatcatt aggctagcat tcggcacgtc cactcccact tcaattacgg
     4321 tggtcgcaac cagcaaatct aaattcgctg ccttaaattc cgccataatc gcttgttttt
     4381 cctgtggttt cagccgtccg tgtactaacc cgatgcgtaa atccggcaag gctcgctgta
     4441 aatcttcggc aatcgctgcc gcggcttgag cctccaacac ttcgctttca tcaatcaggg
     4501 tgcagaccca ataggcttgg cggtgttctt gcttacagac ttggtacacc cgttggacga
     4561 tttctgctcg ccgatcttca gaaatcacca cagtcgtgat tggcgttcta cccggtggca
     4621 gttcgtcaat gattgaggtg tcgaggtcag catagaccgt cattgccagc gttctcggaa
     4681 tgggggtcgc cgtcataatg agctgatgcg gatagttatt ttctttcgct cctttttccc
     4741 gcaaggtcag ccgttggtgt acgccaaaac ggtgttgttc atcaataatc accaacgcta
     4801 aatggtgaaa ttccacattg tcttggaaca gggcgtgagt gccgataatc atctgcacat
     4861 caccgttttt gattgcctca agttgtgcgg ttctcgcctt gcctttcact ttaccggcaa
     4921 gccagccaac ctcaatccca aagggattaa gccagttggc aaaattattg gcgtgctgtt
     4981 cggcaagaat ttcggtcggt gccattaatg ccacctgctt gccgttatca atcgctaata
     5041 gtgctgccaa ggcggctact aaggttttac ctgaaccaac atcgccttgt accagtcgca
     5101 tcatcgggtg tggtttggca agatcttgct caatatctgc ggttactcgg ctttgtgcgt
     5161 gagtaggctt aaaaggcaaa ctgtcgagaa aacgctgttt tatatcggtt tggtaagtaa
     5221 gtgcctctgc atattgctgt tttaccccta aacggagttg ctgcatcgct aaattgtgtg
     5281 ccagcagttc ttcaaaaatc agccgttttt gagcagggtg gtcacctttt tccaagactt
     5341 cggcagaaat cgtcggcggt gggcggtgca ataactgcaa cgcttctttt aggctgtatt
     5401 tgtgcggatt gtattcatca ggcaacagct cggcgacttt cactttctct aataaagcga
     5461 gggcttgctc ggttaatttc cgcagcgagg cttgttttaa cccctcagtg gtggagtaaa
     5521 tcggggtcag ggtttcagcc agtacaagcg gttgattatc ccgaataatt tgatattcag
     5581 ggtggtgaat ttccgccata aatcgcccac gcttaatttc cccaaaggct tttacccgaa
     5641 cgccggtggc aaggctattt ttcattccag cattgaaatt gaagaattta agcataattt
     5701 tgctggtgct gtcggagagt gtaaccgata aaatcgggcg tttaccaaac tggacttcgg
     5761 taagttgtac atagccttca atggtggcaa agctttccgg gcgaacatca gcaatcgggg
     5821 taatacgggt gcgatcttca taccgcattg gcagatgaaa cagtaaatcc tgcacattat
     5881 taatgccgat tcgacttaac ttttcggaaa ttgccgctcc tacgccggaa agggcggtta
     5941 atggcacgcc gtctaataat tgttcactca ttatttatgc ggtttcaaat gggttaaata
     6001 aacgcacatc acaacggata aaatctttgg tgttgcgggt gactaaaatc agattgtggt
     6061 atttggcggt tgccgcaata taagcatcat ttaaatccat tttattggga atatggtact
     6121 ctgatgccaa tagagcaatg tcgttattca ccacaaacgc ttttgtttga taagccttta
     6181 atacattatt ttcaaaccaa tcatttaatc ggtctgattg ttctttgtct ttgcgggctt
     6241 tcagcaaaat acctgttttg atttcagata acgtaataca gctgatattg gtctggctag
     6301 gattaacccc tctcagccac tggcgaacat tgacatccat cttgtcattt cttaacttat
     6361 aaagctcgga aactatattg gtatcaaaaa ggtagcgata cattactcat ctccaaaatc
     6421 aacaggtctt ggtttattgc ttttgcggat ttcaaccaat gtttgttcaa actcttgcca
     6481 aaattcatcg ctcacatcct cattttcgga agaggcgaat agctcttcaa agcttggctc
     6541 gctaggcttt ttctgctgat aatccatata actagacacc acattgacga ttttgcccca
     6601 tttggtaata aaaaccggct ctttttctgc catttttaac actttgctgg cattttgatt
     6661 aaattctctg gaagtaatgg ttattgccat atcgctctcc aaaatgtagt aaaaagatga
     6721 gtgtatttta ttaaatgtag taatatcttt caattaattc gctatttggc aacagatatt
     6781 ttgcaaaaaa tcttgaaaat ctgaccactt gccccatcta ttccctgttt tttaatgaat
     6841 aaaggaatac acaatggcaa ataatcaaga aacccgtggc ttccagtcgg aagtgaaaca
     6901 gttattgcaa ctgatgatcc actcgcttta ctccaacaaa gaaattttcc tgcgtgagct
     6961 tatttccaac gcctctgacg ccgccgataa attgcggttt aaagcccttt ccgagccggc
     7021 tttgtatgaa ggtgatggcg aactgcgtgt gcgtgtctct gttgatgaaa cactcggtac
     7081 gattacgatt gccgataacg gtatcggtat gacccgtgag caggtgatcg accatctcgg
     7141 tacgattgca aaatctggta ccaaagaatt tttgaacgca ctcggcactg atcaagccaa
     7201 agacagccag cttatcgggc agttcggcgt gggcttttac tcttctttca tcgtggcgga
     7261 taaagtgaca gtcaaaaccc gtgccgccgg agtgcctgcc gagcaaggcg tgatttggga
     7321 atctgccggt gagggcgatt acaccgttgc cgatctggag aaaaaagagc gtggcacgga
     7381 aatcattctt cacttgcgtg aggacgaaaa agagttttta aacgaatggc gtttgcgtga
     7441 gattatcggc aaatactccg accatatcgg cctggcggtg gaaattcaga ccaatgaata
     7501 cgatgacgag ggcaaagcaa gcggtcaaaa atgggaaaaa attaacaaag ctcaagcgct
     7561 ttggacacgc tcaaaaaatg agattagtga cgaggagtac accgaatttt acaaacacct
     7621 cagccatgat tttgccgacc cgctgatttg gtcgcacaat aaagtcgaag gtaagcaaga
     7681 atacaccagc ttgctgtatg tgccgagcaa agccccgtgg gatctgttcc aacgcgagca
     7741 aaaacacggc ttgaaactct acgttcagcg tgtatttatt atggacgatg cggaagtctt
     7801 tatgccgaac tacttgcgtt ttatgcgtgg cttgctcgac accaacgacc tgccgctcaa
     7861 cgtcagccgt gaaattctgc aagagaataa agttacccaa tcgctccgtt ctgccttaac
     7921 caagcgtgcg ttacaactgc tggaaaaatt ggcgaaagac gatgccgaaa aatatgccca
     7981 attctggcaa gcgttcggcg tggtgcttaa agagggcgtg ggcgaagact ttgccaacaa
     8041 acaacaggtt gcaggcttac tccgttttgc ctccacccag cgtgatgaca gcgaacaagc
     8101 ggtcagtttt gccgattata ttgcaagaat gaaagagggg cagaaagcga tctacttctt
     8161 aaccgcagac agctaccaag cggctaaaaa cagcccgcac ttagagctgt tccacaaaaa
     8221 aggcattgag gtgctactgc tttctgaccg cattgatgaa tggttggtta gccacctcac
     8281 cgagtttgac ggcaaaccgc tacaaagcat taccaagtcg gacttagact taggcgagct
     8341 tgccgacaag caggaagaag agagccaaaa agcccaacag gcggagtttg gttcgttctt
     8401 agaacgggcg aaaggctact ttggtgaacg ggtcaaaaac gtggtgctga ctcaccgctt
     8461 aaccgacaca ccggcagtgg tttccactga cagcgatgaa atgaccaccc aaatggcaaa
     8521 actgtttgca gcaatggggc aacaagcacc agaagtgaaa tatacttttg agctgaaccc
     8581 agatcaccca atggtgaaaa aagtagctga tcttgccgat gaagccgagt tcgccgattg
     8641 gattgaactg ctgtttgaac aagctctgct tgctgaacga ggcacgttgg aaaacccgac
     8701 agcctttatt aaacgggtaa ataaattgtt gggctagcca ttactaaaaa cggaaatcta
     8761 ggtttccgtt
//
LOCUS       DANLXQ010000106         8622 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.106, whole genome shotgun sequence.
ACCESSION   DANLXQ010000106 DANLXQ010000000
VERSION     DANLXQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.106"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            41..310
                     /locus_tag="PXD94_001849"
     CDS             41..310
                     /locus_tag="PXD94_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL6286686.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            323..1673
                     /locus_tag="PXD94_001850"
                     /pseudo
     CDS             323..1673
                     /locus_tag="PXD94_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(1768..2916)
                     /gene="dprA"
                     /locus_tag="PXD94_001851"
     CDS             complement(1768..2916)
                     /gene="dprA"
                     /locus_tag="PXD94_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL6286687.1"
                     /translation="MAYHYDLLRLLQVPQMGAQRIGRLLAELNFAEFCHYDKLQLKQI
                     GWNEKQIQRWFNPDQKWIDQAMTWSELPNQQIVTLFDENYPFLLKQISTAPPLIYVKG
                     NVESLSLPQIAIVGSRDYSHYGEYWAGQFAAELARHNIIITSGLAIGIDGFAHQKIVM
                     EQGITIAVLGSGLNQVYPARHKKLAEQIVDLGGALVSEFSPNQPPVAENFPRRNRIIS
                     GLSLGTLVIEATLNSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVE
                     GVEDILEVVASQYQPQQIPLFDESKPTVSTSLQAVKNSEKFAKNLPSLTACQQQIYQH
                     ISLEPMAIDDLAKVTELTIEILLVELLGLELAGVIKQVSGGYVMDNGY"
     gene            3005..3517
                     /gene="def"
                     /locus_tag="PXD94_001852"
     CDS             3005..3517
                     /gene="def"
                     /locus_tag="PXD94_001852"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL6286688.1"
                     /translation="MAILDVVLYPDEKLATVCEPVEQVDEALNRFIDDMFETMYEHEG
                     IGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESSGETGIEEGCLSIPGHRALVP
                     RKEKVKVKALNRQGEEIIIDADDLFAICIQHEIDHLNGVLFVDHISNLKRQRIKEKMQ
                     KLKKQIARAK"
     gene            3611..4537
                     /gene="rfaD"
                     /locus_tag="PXD94_001853"
     CDS             3611..4537
                     /gene="rfaD"
                     /locus_tag="PXD94_001853"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL6286689.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(4605..5135)
                     /gene="fabA"
                     /locus_tag="PXD94_001854"
     CDS             complement(4605..5135)
                     /gene="fabA"
                     /locus_tag="PXD94_001854"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL6286690.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(5248..6885)
                     /locus_tag="PXD94_001855"
     CDS             complement(5248..6885)
                     /locus_tag="PXD94_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6286691.1"
                     /translation="MTLSLLSSQKLVMQNDFSRLDEKLNFLDFQPQAKQALNQFLQSQ
                     FGALLVLKSELFPEFINEITEYLKKYSVDLPIISKMDFNKNSLFGYSVFLERELKTEY
                     VKGAIHEADKGILIVNIRSLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTF
                     KLILVGEREDISTLSAYDDGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKR
                     LTSTALNQFLQAYIRESESRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLE
                     QQSSILNQYTVGDILTNQLYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQY
                     GDGEITDIERKVELGGNIHSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYGEIDGD
                     SSSLAIFNVLVSALAKVGLPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLT
                     GKQGVIIPAACVSHLSLSSEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPN
                     SAQTALFTLIHQNLDERESEETSGSFLSKICKKIKLD"
     gene            6978..7424
                     /gene="matP"
                     /locus_tag="PXD94_001856"
     CDS             6978..7424
                     /gene="matP"
                     /locus_tag="PXD94_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL6286692.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            complement(7490..>8622)
                     /gene="ompA"
                     /locus_tag="PXD94_001857"
     CDS             complement(7490..>8622)
                     /gene="ompA"
                     /locus_tag="PXD94_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL6286693.1"
                     /translation="KTLVALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQFDN
                     TYDYGSGINLGINLGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTTFS
                     GDDKEKTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQHVDKDSRIK
                     VHNLKPSLLLGAGLEYAITPELAARVEYQYLNRVGNLDKAVRTTVGYLIPRGTNFQYS
                     PDIHSVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDAAH
                     AEISNLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAVGY
                     GEANPVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
BASE COUNT         2594 a         1785 c         1649 g         2594 t
ORIGIN      
        1 attcagacaa ctattcattc acctttaatg gagatttact atgaaaatta tggcagtatg
       61 tggttccggc ttaggtagca gctttatgat ggaaatgaac attaaaaaag tattggcaaa
      121 actcggtgtc gaagcggaag tgagtcatac cgacctttcc tctgttgttg ctgatgatgc
      181 tgatttattt gtgatggcaa aagatattgc cgcaagcagc aatgtgccga aagaaaaact
      241 catcgtggta aaaaacattg ttggattagc agaattcgaa gaaaaattga ccgcttactt
      301 taaccaataa cggagatgtt atatggaatc gctactcttt tttatattag atattctcaa
      361 agtgccttcc gttctagtgg gtttaattgc tctggtcggt ttagttgcac agaaaaaatc
      421 ggtatccgat attgttaaag gaacaatcaa aactatttta ggcttcttag tgttaagcgg
      481 tggtgcaaca gtacttttaa gctccctgac tcctttaggc ggtatgtttg aacacgcctt
      541 caacgtgcaa ggcattatcc ctaataacga agcgattgtg tctatggcaa tcgaaaaata
      601 tggtacggcg actgcgttaa ttatggcatt cggtatggta gccaatattt taattgcacg
      661 ttttacccgc ctaaaattca ttttcttaac cggacaccat accttctata tggcgtgtat
      721 gatcggcgta atcctaaccg ttgccggctt tgaaggtatt cagctcgtct ttgtcggggc
      781 attaacctta gggttaatta tgtttcccga ctatcgctca cttctatatg aaaaaagtga
      841 ccgctagcaa tgatgttgcc ttaggtcatt tcggcacatt gggctacgtg cttgctggtt
      901 caatcgggca actggtagga aaaggctcta aatctaccga agaaatggat ttacctaaaa
      961 acctcggctt cttacgtgat agctccattt ctatttcact cacgatgatg gcgatttact
     1021 atattctcgc ccttgcttcg ggcagtgaat acgtttctac attaagcggc ggacaacatt
     1081 atttagtgta tgccaccatt caagccatca cttttgcagc cggtgtttat gtcattttgc
     1141 aaggggttcg tttaatttta gccgagatcg tcccggcatt taccggcttc tccgaaaaat
     1201 tagtacctga tgccaaaccg gctctcgact gcccgattgt tttcccttac gcccccaatg
     1261 cggtattagt tggatttctt gccagtttct taggtgggat tttaagctta gccattctag
     1321 ggcaacttaa ttgggtgtta attttaccgg gcgtagtgcc acacttcttc tgtggggcaa
     1381 ctgccggcgt atttgggaac gccaccggcg ggcgtcgtgg tgcaattatc ggtgctttta
     1441 ttcacggcat attaattacc ttcttgcctg tattcttatt acctgtactg ggttcattag
     1501 gctttgctaa caccactttc tctgactcag acttcggcgg tattgggatt gtgttaggct
     1561 atatggctca atacttcgac aagaatatca ttacgctcat tattgtcggc ttattcgctc
     1621 tgttggtagc ctataattat ttggcgaaaa aaccggcaaa actgaatacc taattaaaat
     1681 caggtgttat ttgctgcctg cgaggaatct tccctgccat ttcttgatgg tagggaattt
     1741 tgtttagtta aacgacaacg ctcttgttca atacccatta tccatcacat accccccact
     1801 cacttgttta atcacaccgg ctaattccag acccagtaat tcaaccagca atatttcaat
     1861 cgtcaactcc gtaactttgg ctaaatcatc aattgccatc ggctctaagc tgatatgctg
     1921 ataaatttgc tgctggcaag cggtcagact tggcagattt tttgcgaatt tttcgctgtt
     1981 tttgaccgct tgtaaagatg tgcttactgt tggcttagac tcatcaaaca aagggatttg
     2041 ctgaggttga tattgcgagg cgaccacctc taaaatatct tccacacctt ccacgagcaa
     2101 tgccccttct ttaattagct tgtggcaacc ttgtgcgtaa gggttttgta ccgcattcgg
     2161 taacgcaaac acttccctac cttgttcaag ggcataacga gcggtaatca gtgaaccact
     2221 attaagcgtt gcttcaataa ctaaagtgcc gagcgataag ccactaataa ttcgattacg
     2281 acgaggaaaa ttttctgcca ccggcggctg attaggcgag aactccgaca ccaatgcacc
     2341 acctaaatca acaatttgtt cggctaattt tttatggcga gcaggataga cttgatttaa
     2401 tccactccct aacacggcaa tcgttatgcc ttgttccata acgatttttt gatgggcaaa
     2461 accgtcaata ccaatggcta aaccgctggt aatgataata ttatggcgag ctaattcggc
     2521 agcaaattgc ccagcccaat attcaccata atgagaatag tctcggctgc caacaatcgc
     2581 aatttgtggc agagaaaggc tttccacatt tccctttaca taaattaacg gtggcgcagt
     2641 cgaaatttgt tttaagagaa acggataatt ttcatcaaag agtgtcacaa tttgctgatt
     2701 tggcaattct gaccaagtca tcgcctgatc aatccacttt tgatcaggat taaaccaacg
     2761 ttgaatttgc ttttcattcc aacctatttg tttgagttga agtttgtcat agtggcaaaa
     2821 ttcagcaaaa ttgagttccg ccaataatcg ccctatacgt tgagccccca tttgcggcac
     2881 ttgtaataat cgtaataaat cgtagtgata tgccatattt tcatcctcaa aatttcccta
     2941 ataataagaa aaggtttaaa atagccaaac gaatttttta aacttttcga gagagatcac
     3001 aaaaatggca atattagatg ttgtgcttta ccccgatgag aaactggcaa ccgtttgtga
     3061 accggtggaa caggtggacg aagcactgaa tcgttttatc gatgatatgt ttgagacaat
     3121 gtacgaacac gaaggtattg gcttagctgc accgcaagtg ggcgtactta agcgtgttat
     3181 tactattgat attgagggcg acaaaaccaa tcaagtggtg ttaattaacc ctgaaatttt
     3241 agaaagcagc ggtgaaaccg gcattgaaga gggctgtctc tccattcccg ggcatcgtgc
     3301 cttagtgcca cgcaaagaga aagtgaaagt caaagccctt aaccgccaag gcgaagaaat
     3361 tatcatcgat gccgatgatt tatttgcgat ttgtattcaa cacgaaatcg accatttaaa
     3421 cggcgtgctt tttgttgacc acatatccaa tttaaaacgc caacgcatca aagaaaaaat
     3481 gcaaaaattg aaaaagcaaa ttgcacgagc gaaataacca ggcacaagcg gttaaaatgg
     3541 cttaaaaatt tgcaaattaa gtgtgatgac acgataataa ccacagttca actttagaaa
     3601 ggataaaaat atgattatcg tcaccggcgg cgcaggtatg attggcagta acattgttaa
     3661 agccttaaat gaaatcggtc gcaaagatat tttagtagta gataacctca aaaacgggga
     3721 aaaatttatc aacttagtgg acttagatat tgcggattat tgcgataaag aggatttcat
     3781 cgcctctatt atcgccggcg atgattttgg tgagattgat gcggttttcc atcaaggggc
     3841 ttgctcggca accaccgaat gggacggcaa atacttaatg caaaataact acgaatattc
     3901 aaaagagtta ttgcattttt gcttagatcg ccaaattcct ttcttctatg cctcaagtgc
     3961 ggcaacctat ggcggacgtt cggataattt tatcgagaaa cgggagtttg aaggtccatt
     4021 aaatgcttat ggctactcca aattcctgtt tgatgaatat gtccgccgta ttttgcctga
     4081 ggcagaatca ccggtctgtg gtttcaaata tttcaacgta tacgggcctc gtgagcaaca
     4141 caaaggttca atggcaagcg tggcattcca cttaaacaac cagatgttaa aaggggaaaa
     4201 tccaaaacta tttgccggct cagagcagtt cttgcgtgat tttgtttatg tggaagatgt
     4261 ggcaaaagtg aatctttggg cgtggcaaaa cagcatttct ggcattttca acctcggcac
     4321 cggaaaagcg gaatctttcc aagaagttgc gaatgcggtt atcaaatacc acggcaaagg
     4381 cgaaattgaa accatcccat tcccggatca tttaaaatcc cgctaccaaa cgttcactca
     4441 agcggactta accaacctaa gagccgctgg ctacacaggc acatttaaaa ccgttgctga
     4501 aggaacagcg gaatatatgg cgtggttgaa tcgctaatca aacgttgtta aaaaagcaga
     4561 aagagctacg aaatgtagct ctttttgtta taaggttata aggattagaa tgctgtcgta
     4621 tcttggaata agcctacttt taaatcagta gcagtataga tcactcgccc gtccacttcg
     4681 acttcaccat cagcaacccc catcactaac ttacgattga ccacacgttt catatggata
     4741 cggtaagtaa cttttttcgc tgtcggtaaa atttgtccgg tgaattttac ttcacctacg
     4801 cccaatgcac gccctttgcc ttcgccacca atccagccga gataaaaacc aaccagttgc
     4861 cacatcgcat ctaagcctaa gcaacccggc attacaggat cgttaataaa atggcagtta
     4921 aaaaaccata aatccggttt aatatcaaac tcagcttcaa tatacccttt ttcaaaattg
     4981 cctttttctt ggttcatttc aatgatgcga tccatcatta acatcggtgg agccggcaat
     5041 tgaggccctt tttcaccgaa taattctcct tttcctgata gcaaaagctc ttcgtaagta
     5101 tagcttggtt tgataattgg tgtacaactg ttcattattt ttaaaatcct gtaaagtgaa
     5161 aaaagcctgt tatatcaata actgataaat caggctgata gtaacagact tttaattcag
     5221 tgaacagttg taagctgact tgtcggatca gtctagcttt atctttttgc aaattttgct
     5281 taaaaatgaa ccgcttgttt cttcactttc ccgctcatct agattctggt gaatcaaggt
     5341 aaataatgct gtttgggctg agttaggggc tttctcatca tcataaaaat cccgctcaaa
     5401 cagaatcgct atggcatcaa acacattttc caccgtccaa attttaaata gaccttgctc
     5461 cactgcctca acaacttctg aacttaaact caaatggcta acacaggccg ccggaataat
     5521 cacaccttgt ttccccgtta attggcgagc gtgacaaata ttaaagaagc cttcaatttt
     5581 ctgatttacc ccgcccacgc ttagcacatt cccaaattga tcgatagcac ctgtcaccgc
     5641 aatagattgt ggcaagccaa ctttagctaa ggcactcact agtacattga aaatcgcaag
     5701 agaagaacta tccccatcaa tttcaccata ggattgttca aaagcaatcg aagctgaaaa
     5761 cggtaactgg gttggtagtt ctaacagatt cgctaaacaa gattgagcga taataatacc
     5821 tttggagtgg atatttcccc ctagctccac tttacgctca atatctgtaa tttcgccatc
     5881 accatattgt acattacaac tcatgcgtaa gggctcgcca aaactatgcg gaatgccttc
     5941 aaactcaatg accgataagc cattgatctg cccaatttcc tctccttcgg tttcaatata
     6001 aagttgatta gtcagaatat cacccaccgt atattgattt aatatcgatg attgctgttc
     6061 taaataatca aaataacctt ttacctcgtc aatttcggtc acatttgggt aaaaattttg
     6121 aattcctaat aaatgttttc tcaataatgt aggggaaata gacaccaact ctcggctttc
     6181 actttcacgt atataggcct gtaaaaattg attaagcgct gtggaagtta atcgcttatt
     6241 gatgagattc tcagcataat tctgaacata acctccccac tgctccacat tatccgataa
     6301 tgataagtag gattttattt cggtatattg cccaaattgg tacaacccat catcataagc
     6361 agataaagta gaaatatctt ctctttcccc tactaaaatc aatttaaatg ttgatttctc
     6421 tttagccgat aaatagggtg agtgattaac agaatttggc tcatattcac caaataataa
     6481 tgcctgtttt agcttatccc attgggtaat atccaataag agcgaacgga tattaacaat
     6541 taaaattccc ttatctgcct cgtgaattgc acctttaaca tattctgttt tcagctctct
     6601 ttccaaaaac acagaataac caaatagact atttttattg aaatccattt tgctaataat
     6661 cggcagatct acactatatt ttttcagata ttccgtgatt tcattgataa attccggaaa
     6721 aagctcagat tttaagacta ataaagcacc aaactgagat tgtaaaaatt gatttaacgc
     6781 ttgttttgct tgaggttgaa aatctaaaaa attcaatttc tcatctaaac gagagaaatc
     6841 attttgcatt actaattttt ggctactaag taaagaaagg gtcacaaatc tgcctcatta
     6901 ttgaaaattt ggtttattat acaaaattcc gctatacttg ctaaatatag cttacttttt
     6961 cctatcatat tcagattatg cgatatcaga aactcatcac ccaagaaaca acttggaaat
     7021 ggcaatatct cctaaaaaaa catcgtgaag gagaaaatat tactaagtac gaagaacaaa
     7081 gcttgatcga tttaaaagtt cagttcctct ctactttgca aaactctccg gacgaagtgg
     7141 aaaaatggat taagtcggaa atgacgcctg agcaacgtaa gaaaatgcgt caatcagtac
     7201 gcgccaaacg caaacgcttc tttaatgctg aaaagcaaac aaccaagaaa aaatccattg
     7261 atttagagta ttcgagctgg ttaaggctct ctaaatatgc caaaagccaa aaactaacgc
     7321 tttctgaagc tattgtcaaa ttaaccgatg aactcgaaaa taaacaactt tacttagagc
     7381 aagttgctaa aatgaaatca agtttaaaag atttactgaa ataaaaaaag aaaaaagccc
     7441 actaacaagc gggctttttt tgattcaatt tttcaactga ataactatat tacatagtta
     7501 cttcttttga accttgaact tggatttcaa cacgacgatc tggtgctaag caagcgatta
     7561 atgctttacg acctttaact gcatcacatg tgtgaccagt tactgggttt gcttcaccgt
     7621 aacctacagc tgttacatta gctgggttag tacctttaga aacgatgtaa ttagctactg
     7681 tttcagcacg acgttgtgaa agttttaagt tagatgcttc tttaccgatg cggtctgtgt
     7741 aaccattaac ttggatagct aggtttgcta agcctaagtt gctgatttca gcgtgtgctg
     7801 catctaaaga agttgctgct gctggtttta agcttgattt accgaaatca aataaaacat
     7861 cagagctgaa tgcgaagttt ttagttacaa cttctggtgc ttcaactggt gctgcacctt
     7921 gaccgaaacg gtatgataaa cctgcagata ctgagtggat atctgggctg tattggaaat
     7981 ttgtacctct tggtattaga taaccaactg ttgtacgaac tgccttatct aagttaccaa
     8041 cacgatttaa gtattgatat tcaacacgag cagctaattc aggagtaatt gcatactcaa
     8101 gaccagcacc taataataga gatggtttta agttgtgaac tttaatgcgt gaatctttat
     8161 ctacatgttg ttgtacgtag taatcgttgc gaactaatgc aacacctact ttaccgtaaa
     8221 catctaagtt aggaacaact tcgtagcttg gttttaagct taagtgcgca ccgtgagctg
     8281 aatgtttagc cgcagttttt tctttatcat caccagaaaa tgtagtgtta ccacgaacac
     8341 gaccaaagta gtcataacct aattctgtcg ctaaaccaaa gttgttttgg tttaagattt
     8401 ggtaaccacc gaatacaccg taagttacag agttacggtt aataccgagg ttaataccga
     8461 ggttaatacc ggatccataa tcatatgtgt tgtcaaattg agttaaaccg tcgtggaatg
     8521 atgcccaacc agctttagca cctgcgtaga aagtgttagc ttgtggagct gcttgagcta
     8581 ctgcagctgc tgataatact gctaatgcaa ctaatgtttt tt
//
LOCUS       DANLXQ010000107         8400 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.107, whole genome shotgun sequence.
ACCESSION   DANLXQ010000107 DANLXQ010000000
VERSION     DANLXQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.107"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            28..3186
                     /locus_tag="PXD94_001858"
     CDS             28..3186
                     /locus_tag="PXD94_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL6286694.1"
                     /translation="MITINENTIEQSAIATLQSLGWDYTYGKKILAGLEHEWRERTAE
                     VILKPLLAQAIAKFNPNLPACEVENVVAQVCRADSGDLAERNRQAYDWLRNGVKITYQ
                     LYGEQVSDVVQLIDFQRPENNDFRIVNQLDISGKKGKRIPDLIGFVNGLPLVVFELKN
                     PLKENADIGKAFAQLQTYKDEISDLFVFNQALVISDGIVARIGSLTADFDRFTPWRVV
                     DEKNQSKRIVFEDELTALLQGVMTPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYG
                     VNEAVDCTLLAATGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQSSLKNPTLVLVTDRN
                     DLDGQLYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELA
                     HPVLNERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTP
                     IELDDKDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFSKVMEEAQQL
                     IDDDENSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKL
                     YEKITALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNI
                     VIVRDMWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGL
                     TDELEKAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIHGQFATPIDGQAVDIAKMLQ
                     ISEPPKLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGAL
                     KAVEPYKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFD
                     LLKKDRPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQK
                     LKEAMNQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESA
                     VREMGDEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAE
                     AIEFVLQQAEEIAGEFAITETA"
     gene            complement(3220..6111)
                     /gene="glnE"
                     /locus_tag="PXD94_001859"
     CDS             complement(3220..6111)
                     /gene="glnE"
                     /locus_tag="PXD94_001859"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL6286695.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKSIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKILQERSLLKVLPRLAELNLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPT
                     DEASQARLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIG
                     EEETQANCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQ
                     DWVKRPIGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLE
                     KEQILPQLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRI
                     PEEDEEALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWK
                     QVSQRFGVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKE
                     ISSHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEA
                     WTWESQALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSS
                     HSAEQFHIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSE
                     QSEQLQHCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
     gene            complement(6283..6564)
                     /locus_tag="PXD94_001860"
     CDS             complement(6283..6564)
                     /locus_tag="PXD94_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL6286696.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYSDVIIKPFKRVF"
     gene            complement(6566..7030)
                     /gene="yciA"
                     /locus_tag="PXD94_001861"
     CDS             complement(6566..7030)
                     /gene="yciA"
                     /locus_tag="PXD94_001861"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL6286697.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(7020..7577)
                     /locus_tag="PXD94_001862"
     CDS             complement(7020..7577)
                     /locus_tag="PXD94_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL6286698.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(7586..8395)
                     /locus_tag="PXD94_001863"
     CDS             complement(7586..8395)
                     /locus_tag="PXD94_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6286699.1"
                     /translation="MAQALLYFTTKYLQSIEGYFMSIKFVDLLQQSWNFMRNQQAFSL
                     FAIVTIVVVQLVFILVSPNSPELMAESQPQQLQIDASKMVSILLPTILLGVVNLLINV
                     LMIFNIQSINDGNYRQFFQNAGNALKHFLPVLLLQFVMVLPLSLGASFAMASPETVII
                     ALPVLVVGFYFFIKLSLVIYAYLLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYL
                     LPGMLSRLFTVFGNDFIGIFITIVLSAVINVFMAIFSFRFYQVYRQMPAVR"
BASE COUNT         2471 a         1829 c         1804 g         2296 t
ORIGIN      
        1 attattaagt ggggagatgg agttgtgatg attaccatca acgaaaacac cattgaacaa
       61 tccgccattg caactttgca aagcttgggc tgggactaca cctacggcaa aaaaattttg
      121 gcaggtttag aacacgaatg gcgggagcga accgctgagg tgattttaaa gccgcttttg
      181 gcgcaagcga ttgcaaaatt caacccgaat ttgcccgctt gtgaggtgga aaatgtggtg
      241 gcacaggtgt gccgtgccga cagtggcgat ttggcggagc gtaatcgtca ggcttatgat
      301 tggctaagaa atggggtcaa aatcacttat cagttgtacg gtgagcaggt gtctgatgtg
      361 gtgcagctga ttgattttca gcgtccagaa aataacgatt ttcgcattgt caatcagctg
      421 gatattagcg gcaaaaaagg caaacgcatt ccggatttga ttggctttgt taatggcttg
      481 ccgctggtgg tgtttgagct gaaaaatccg ctcaaagaaa atgccgacat tggcaaggcg
      541 tttgcccaac tgcaaaccta taaagatgaa atttctgatt tgtttgtgtt taatcaggca
      601 ctcgtgattt cagacggcat tgtcgcccgc atcggttcgc tgaccgctga tttcgaccgt
      661 tttacccctt ggcgtgtggt tgatgaaaaa aatcagagca aacgcattgt gtttgaagat
      721 gaactcaccg ccctgctgca aggcgtgatg acacccaaaa atctcttgga ttatgtgcag
      781 aattttgtgg tgtttgaacg ggacggcaaa aaccgcttaa ttaagaaaat cggagcatat
      841 catcagtttt atggcgtgaa tgaagcggtg gattgcacct tgcttgccgc cacaggcaac
      901 cgcaaaatcg gcgtgttttg gcatacgcaa ggttcgggca aatcgctttc gatgctgttt
      961 tatgcaggta aagtattaag ccaaagcagc ctgaaaaatc caactttggt gttggtaacc
     1021 gaccgcaacg atttggacgg tcagctttac gccacttttt gcggtggcga ggcactgctc
     1081 aaacaaacgc caatccaagc cgatgggcga gacgaactcc gctctgccct tgccagtcgt
     1141 tcggcaggcg gtgtgatttt taccaccatt caaaaattcg gcttaatgga gggtgagctt
     1201 gcccaccctg tattaaacga gcgggaaaac atcattgtga ttaccgatga agcacaccgc
     1261 tcacaatatg gttttagcca aaaaatcaac cacaaaggcg agtatcgtga gggttatgcc
     1321 aagcatttac gcagtgcatt gccgaatgcc tcttttattg gctttactgg cacaccgatt
     1381 gaacttgatg acaaagacac ccaagaagtc ttcggtaaat atgtctcgat ttatgatttt
     1441 gaagatgcgg tggaagatgg ggcaaccgtg ccgattattt atgagccacg ccaaatcagc
     1501 ttaggcgaaa gtggcgagtt ttccaaagtg atggaagagg cacaacaact gattgatgac
     1561 gatgaaaaca gctataactt ccgcctgcgt gaaaaactgc acagcgtgga tagccgtttg
     1621 caaaaaatgg cggaagatat tattgcccat tacgatgagc gtaccaaaca gcaagacggc
     1681 aaggcgatgg ttgtggtgat gagccgtgcc atttgcgtga aattgtacga aaaaatcacc
     1741 gcacttcgtc cagaatggca ctcaaacgat gtgcttcaag gcagcattaa aattgtgatg
     1801 acaagtaatg ccagcgaccc tgccgagtgg caaaaacaca atcaggataa gaaaacctta
     1861 gaaaaacgct ttaaagaccc agacgatccg ctcaacatcg tgattgtgcg ggatatgtgg
     1921 ctgacaggct ttgatgcgcc ctgctgtaac acaatgtaca ttgataaacc aatgagcgga
     1981 cacaacctga tgcaggcaat cgctcgggta aatcgggtat ttcgcaacaa aagccgtgaa
     2041 aatggcggct tgattgtgga ttatgtgggc ttgaccgatg agctggaaaa agcgatgaag
     2101 cagtacacca acgcaggcgg taaagaaaag ccggtgcggg acatttcagc ggtgctggaa
     2161 aaaatggtgg aacatatcac ggtcattcat gggcaatttg ccacaccgat tgacggacaa
     2221 gcggttgata ttgccaaaat gttgcaaatc agcgaaccgc ctaaactgct caatgcgatt
     2281 ttgacatcag ccaaccatat tcttgcccta gaccgcattc agccacctga taacactgcc
     2341 aaagacaaaa ccccacgcaa aaacgccttt ttgcaatcgg tacgcttggc gaaaaaaggt
     2401 tatgctttgt gtggggcatt aaaagcagtt gaaccctata aacaggaact ggcgttttat
     2461 gatgcagtgc gtgccaccat tatcaaaaac agcaccgctc ctcgcaattc ttcgagcgaa
     2521 aatgaccgct tgttgcagct taccgccttg atgaatcgtg cggtacagtc ggatggtgtg
     2581 gtggatttat ttgatttgct gaaaaaagac cgcccaaaca tcaacctgct ttctgatgag
     2641 tttttggaaa cagtaaaaaa cagcccaacc aaagatttgt ggctatcagc aatggagcgt
     2701 tatcttgcct cacaactgcg agagcaaagc ggtgccaatc ttgccacaaa aaaagcattt
     2761 gaacagaaac tcaaagaggc gatgaaccaa taccacaacc acaatttaag cgtattggaa
     2821 attttagaag agctgatcgc cctcgccaaa gagtttgaag cccgccaaaa acgaggggaa
     2881 gcgttgggac taagccctgc ggaaatggcg ttttatgatg ctttggcacg taatgaaagt
     2941 gcggtgcggg aaatgggcga tgaggtattg atgaaacttg ccaaagacat taccgataaa
     3001 ttacgcaaat cggtcaccgt agattggcaa tataaagact cggttcgtgc caaaatgcga
     3061 accttaatcc gcattgctct gcgtacctat aaatacccgc ccgatttaca ggcggaagcg
     3121 attgagtttg tgttgcaaca agcggaagag atcgccggag agttcgctat caccgaaacg
     3181 gcgtaatcta tattagggcg tgcaatgtac gccctaaatt tatgctaata acctctgcca
     3241 catttcccgc acaaatgctc gctcggtggt aaattcactt gcgtccacca cgctgtcttt
     3301 gcgtaataag tttaggtggt ggattttgtt acgcagtgcg gtgtagcaat gttgtagctg
     3361 ttcgctttgt tcggaactta aaataccgca ttctactgcg ctgtcgaaaa ttcggacatt
     3421 atccgaccat actgccattt tcgggtgttg gtggctgtgg gctaatacca aatattgggc
     3481 gataaattca atatcggtaa tacctccttg atcttgttta atatgaaatt gctcggctga
     3541 atgcgagctt aaatgttggt acattttctg tcgcatttcg caaatttctt ggcgtaattg
     3601 accgcttgca cgaggctgag caagcgtgct ttggcggatt ttttcaaagg cttgtttgag
     3661 gttttccgcc ccaaatacac atcttgtccg cacgagggct tggctttccc acgtccacgc
     3721 ttcatttttt tgataatgct cgtaggcatt gaaagtgctg actaaaagcc cagcctcgcc
     3781 tgatgggcga agtcgcatat ccacttcata tagtacgccg gcactggtat taaggttaaa
     3841 aatcgagtta attttttgag ccaattttaa gtaaaattga tggcttgaaa tctctttttt
     3901 accgccaaca gtttggctat cctctggggc attatgcaag aaaaccaagt cgagatctga
     3961 attatagccc agttcaatcc cgcctagctt gccgtagcca ataactgcaa accctttttc
     4021 atcagcctct aaatgttccg gcacaccaaa gcgttggctg acttgcttcc acgccatatt
     4081 taccaccgca ttaataatcg cttctgctaa gtaagtaaga tgatcgctga tcttcattac
     4141 cagcagcacg cctaaaatat cagcagcggc gatgcgtaaa atttgggttt gtttgaactg
     4201 acgtaagcca tcaatcagtg cttcttcatc ttcttcagga atgcggatta aataatcctg
     4261 caaggcagat tgatattttt caaaagcctg cacgtcggtt aagcctctat ggctcatcag
     4321 ctcatcaagt agcattggat atcgagctat ctgttccgca atcataaccg acttactaca
     4381 caatgctaaa agttgaggca gaatctgctc tttttcgagc attaattcca aataggtagt
     4441 tcgggtggtg attttatcga caatattcaa cacgcgaggc aataacaata aatgatcttc
     4501 acctttaaaa atgctgtcgg caattctagg cattaaattg cgtagcacct ctcgccctct
     4561 taccccaatc gggcgtttta cccaatcttg aaaggtagta gaaaagtgac ggaaaatctc
     4621 cgtataatca ttttctgcaa cctttggata accttttagc actgcggata aatcttcaag
     4681 gcgaatgtta tagtgcaaaa tgtctttcca ttcggctaat ttttcattaa ccggattgca
     4741 gttggcttgt gtttcctctt caccgataat ttcattaaaa atctggcgta ctgcttgttg
     4801 atattggctc aatactgcca taaattgttg ccaatcgtta aaggaatgct ccacccaatg
     4861 cgttttttcg ctatatagat cttgctgata atagctttgg caagcaaata caaggcgggc
     4921 ttgcgaggct tcatcagtgg gtagggtttg agtttgttta tcgtcaatcg cttgtaatac
     4981 attttcaatt tgacggtaaa aaatataggc attgtggagc gtttccacct gcgtttgggt
     5041 taataaattt aattctgcca agcgtggtag cacttttaac aggctacgtt cttgtaaaat
     5101 tttatctcgc ccgccacgga tcatttggaa agtttgggcg ataaactcca cttcccgaat
     5161 cccaccggca ccaaatttaa tattatcggt taaattacgg cgaaccactt cacggctgat
     5221 tttttctttc atttcacgca acgattggat tgcactgaaa tccagataac ggcggtaaac
     5281 aaaagggcgg agcatttgct ttaaatagcg atgatttaaa ttttggctat cctcgcctaa
     5341 aatttttgct ttaatcatcg cataacgttc ccaatcacgc ccttgctctt ggtaataatc
     5401 ttccattgca gtaaaactca gcaccaaggc tccgctatca ccaaaggggc gaagtcgcat
     5461 atcggtacga tacacgaaac catcagcggt gatctcatct aatactttaa ttaaccgctg
     5521 ccccattcgg gtaaaaaatt tgctgttttc aatcgatttg cgtccacctt cggtttcacc
     5581 aatatcgggg taggtaaaaa tcaggtcaat atcggaagaa aaattaagct ctcgtccgcc
     5641 caatttgccc atacctaaaa tcagcagttc ttgtgtttcg ccaagagtgt tctttggcgt
     5701 gccatattcg gcacagcaac gctgaaacaa ccaatctcga gcggctagaa tcaggctctc
     5761 ggctaaatcc gacaaatgct gaaataccaa ttctaccgtc gctaatttat ttgactgaat
     5821 aaagctcaga cgagccaatt cacggtggcg aaattgccgc aaaaccctcc ccaacatttc
     5881 ttcattttcc actcttgcta atatttttgc taaacgctca gtataattgc agcaatcatc
     5941 aggcgtagga aacttggcaa gccattctgc cagtaaatcc ggctgttttt gcaaggtttg
     6001 atgcacgaaa ttggaaagcg aaatcgccgt cacaagcggt tgaatttgct cagaattttg
     6061 caaattgggc ggtaaattga gttgggataa gggaattagg gtggtaagca taaatttagg
     6121 ctcaaaattg aaaatagata gaattttacg ctcgatcaat tcccctcttt agcaaagagg
     6181 gcttagggga gatttggcag aggtaatttt actctcatac gaaacattaa tatcgtaatc
     6241 aaatctcccc cagcccctct tttctaaaga ggggagaaaa aattaaaaca ctcttttaaa
     6301 cggtttaata atcacatcgc tatacacacc ggcttccaca taaggatctt ggcttgccca
     6361 ttgttgagct tcttcaagtg agttgaactc tgcaattaca gttgaaccgg ttacgcttga
     6421 gccgtcttca gtcgggttag gccctgcgat taataatcga ccttctgcct gtaatttttc
     6481 caaacgagcc aaatgagccg ggcgaacttc taaacgtttt tccaacatat tgggattatc
     6541 ttgggcaaag ataacataat acatactatt ctccttgttt ttcaggggtt aaatcaatta
     6601 gcgataatgc ttcgtctaat tcggggttat tttctctcgg cacagcacgc ggtttgcctt
     6661 ctttatcaat cgctacatag gtaaataagg cttcggttac gagataacgt tcgcgattgc
     6721 cctcataaac ttgtttgata aagacctcga ctttcacttg catcgaagaa cgccctatgt
     6781 gcattaactt gccgtagcag cagaccacat caccgactga aatcgggcgg tggaaaatca
     6841 ttttatctac cgttaccgta acgactctac cttttgccaa ctctttggct aaaattgcac
     6901 cgcctaaatc catctgcgac ataatccaac cgccgaaaat atcgccattg gcgttagtat
     6961 ctgacggcat tgccaaagtt cttaaaatca gtttcccttc cggggctttt ccatagggtt
     7021 tatctttcat ttttttgttc ctgcttttca gcatctttta agtaagggta aaggacgaaa
     7081 ccggtaataa ccgttgcgac taaggttaag ccaataatgc caaaggattt gaaatcaacc
     7141 caaatctctt ccgataaata ttgactgata taaatattca gcagcataca aatcagaaag
     7201 aatactgccc aaccgaagtt tattttattc cacactgctt caggcaactg aatttctttg
     7261 cctaataatt ttttgattaa tggtgttttg aacacaaatt ggctcactaa tagcaccaat
     7321 gcaaacagag cgtaaacaat ggttactttc cattgtaaat atttaacttc attaaaatag
     7381 gcactcagta agccgaaaaa gaccaccgag ccacccataa taatttgctg tttttcaatt
     7441 ttgccgtaga gcatttttaa ggcgatcatt tgcacgattg tagcgataac caatgcgata
     7501 gacgccatct gaataccgct cattttatac acgacaaaaa acagaatcag cggaataaat
     7561 tcaagtaact gtttcattga tactcctaac gcactgccgg catttggcgg taaacttggt
     7621 aaaagcggaa gctgaaaatc gccataaaga cgttaattac cgcacttaaa acaatggtaa
     7681 tgaatatgcc aataaagtca ttaccaaaca ctgtgaacaa acgagataac atccccggta
     7741 ataaataaga aatcacacaa aataaaatca gcggtagcat ttttccacgt gataattgaa
     7801 gcgtaaattt aatactttcc gatagacttt tctgcggttt ctccaataaa taagcataaa
     7861 tcactaacga taatttgata aaaaaataaa aaccgactac caacaccggc aatgcaataa
     7921 tcactgtttc aggcgatgcc attgcaaaag atgcaccgag agaaagcggt aacaccatca
     7981 caaattgtaa caacagcaca ggtaggaagt gctttaatgc gtttcctgca ttttggaaaa
     8041 attgacgata attgccatcg ttaatcgatt ggatattgaa aatcattaag acattgatca
     8101 aaagattgac aacacctaat aaaatagttg gcaatagtat agacaccatt ttagaggcat
     8161 caatttgaag ttgttgcggt tgcgactccg ccattagctc aggcgaattt ggcgacacta
     8221 aaataaaaac caactgcaca accacaatgg tgacaatggc aaacagtgaa aatgcttgtt
     8281 gattacgcat aaagttccaa ctttgctgca ataaatcgac aaatttaatt gacataaaat
     8341 acccttcaat agattgtaaa tattttgttg tgaagtatag caaggcttga gccaaactgc
//
LOCUS       DANLXQ010000108         8337 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.108, whole genome shotgun sequence.
ACCESSION   DANLXQ010000108 DANLXQ010000000
VERSION     DANLXQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.108"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(340..2010)
                     /gene="glnS"
                     /locus_tag="PXD94_001864"
     CDS             complement(340..2010)
                     /gene="glnS"
                     /locus_tag="PXD94_001864"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL6286700.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQDYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
     gene            complement(2171..2614)
                     /locus_tag="PXD94_001865"
     CDS             complement(2171..2614)
                     /locus_tag="PXD94_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL6286701.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVPFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            complement(2665..3240)
                     /locus_tag="PXD94_001866"
     CDS             complement(2665..3240)
                     /locus_tag="PXD94_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL6286702.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            3347..4237
                     /locus_tag="PXD94_001867"
     CDS             3347..4237
                     /locus_tag="PXD94_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6286703.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            complement(4304..4870)
                     /gene="efp"
                     /locus_tag="PXD94_001868"
     CDS             complement(4304..4870)
                     /gene="efp"
                     /locus_tag="PXD94_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL6286704.1"
                     /translation="MASYSTNDFKKGLKFIQDGEPCVIVENEFVKPGKGQAFTRTKIR
                     KLISGKVLEINFKSGTSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGD
                     NVKWLVDNAECIVTLWNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGA
                     VVNVPLFVQIGEVIRVDTRSGEYVSRVK"
     gene            5037..5606
                     /locus_tag="PXD94_001869"
     CDS             5037..5606
                     /locus_tag="PXD94_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286705.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVANGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
     gene            5660..6652
                     /gene="epmB"
                     /locus_tag="PXD94_001870"
     CDS             5660..6652
                     /gene="epmB"
                     /locus_tag="PXD94_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL6286706.1"
                     /translation="MQPIQIKPLWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
     gene            complement(6752..8155)
                     /gene="asnS"
                     /locus_tag="PXD94_001871"
     CDS             complement(6752..8155)
                     /gene="asnS"
                     /locus_tag="PXD94_001871"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL6286707.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLHLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKNYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVLDQRMNEMGLNPEDYWWYRDLRKYGTVPHSGFGLGFERLIVYVTGLQ
                     NIRDVIPFPRAPRNANF"
BASE COUNT         2391 a         1781 c         1731 g         2434 t
ORIGIN      
        1 aaaagagcat cacttttggt ttccaaagat tatagaaagc tcaaataata cactacctca
       61 taatttaaaa tgaaacgttt caattggaaa aagtggattt tgtgactgag atcacaaatt
      121 taaacattgg tctgtatttt tgagagattt tgtttgaaac tgccggcaaa ttcgcttaac
      181 cttaaggtaa caagaataag tgttgtacct agcaacctaa cacataaatt tgctttccga
      241 gtggccccaa gaattggggc tttttttatg cttgatgagt cgttttgaga gtaaaaaaat
      301 accagcatat ttgctggtat ttgaatgaag agagttagat tagaacgcta cgctttcttt
      361 taagctgaca gttaagttaa atacaaggtt gtctgccgaa ccatctttgc tgtctaagca
      421 gtagtaacct tcacgctcga attggtagtc ttgttccggt ttggcgtgag ctaaacttga
      481 ctcaacaaaa ccatgcttaa ccactaatga ttcaggattt agcaccgcat tgatgtcttc
      541 ttccgccccc gggtttggta ctgtaaatag gcggtcgtaa atgcggaact ctgccggttt
      601 gttatcttca gcagataccc aatgaatcac gccttttact ttgcgaccat cagccggatt
      661 tttgcccaat gtatccgggt cgtatgtgca aaaaatagtt gaaattcgac cgcttgcatc
      721 tttttccaca cgctcagctt taatcacata ggcattgcgt aaacgaactt ctttgcctaa
      781 gactaaacgt ttatattgct tattggcttc ttcacggaag tcggcttcat caatataaat
      841 ctcacgggtg aacggtaaat cacgctcgcc aagctctgga cgatttgggt gattaggggc
      901 ttttaatatt tccttttcac cgaagttttc aatcaccact cgaaccgggt taatcaccgc
      961 catcgcgcgt ggtgcgtttt catttaagtc ttcacgaatg cacgcttcaa gggcagagta
     1021 ttccaccacg ttatcttgct tggttacccc aatacggcga caaaattcac gtaaagacgc
     1081 tggggtataa ccacggcggc gtaagccgga aatggttggc atacgagggt cattccaacc
     1141 atcaacaatg ccgtctgtga ctaattttaa taatttacgc ttagaggtta aagtaccttc
     1201 taaatttaaa cgtgaaaatt cgtattggtg cggtaacggt ctttcaatcg aaatattgtc
     1261 cagcacccaa tcatataagc ggcggttatc ttggaactct agcgtacata gcgagtgagt
     1321 gattcgctca atcgcatcgg aaatgcagtg ggtaaaatcg tacatcgggt agatgcacca
     1381 tttatcaccg gtttgatgat ggtgagcaaa tttcacacgg taaagcaccg gatctcgcat
     1441 cacaataaac ggggacgcca tatcaatttt tgctctcaag caggctttcc cttccgcaaa
     1501 ctctccgttt ttcatttttt cgaataacgc taagttttct tccacgctac gatcacggta
     1561 agggctgttt ttgcccggct cggttaaagt accacggtat tcacgcattt catcaggcga
     1621 taattcatca acataggcta atcctttgtt gattaactca atcgcatagc cataaagctg
     1681 gtcaaaatag tctgaggcat aacgaggctc accctcccat ttaaaaccta gccactccac
     1741 atcttgctta attgaatcta catattccac gtcttcttta accgggttgg tatcatcaaa
     1801 acgcaagtta cataaaccgt tataatcttg agcgatacca aaatttaaac agatagattt
     1861 agcgtgtccg atatgcaaat aaccgtttgg ttcaggtgga aaacgagtgt aaacattgtt
     1921 gtgctttccg cttgctaaat cttcatcaat aatatgggta ataaaattgg ctcgagtgcc
     1981 agtttctgcc gttaaaattt cttcgctcat agttctctct aaatgtatca ataaaaataa
     2041 cggattattc tacacttaat tgaaacagac ttaaagacta aagcaaaatt cctcggtttc
     2101 ctccaaagaa ttttgctgtt ttagtaagat gcaatggaat tgcaaatttt tagctaaatt
     2161 taaccgcttg ttattgcctg atttcacggc tttcgctgat cataaaattc gcaatccgtt
     2221 tggcaatccg ccatttaccc tcttgtttaa cataggtatc ggcataacgc acgctgtgat
     2281 cgtgcaagat ctctttgccc tctttggttt ctaccagttt caccgcacaa tagttgatcg
     2341 ctgtggcgtt attttcatca ataaaaggca cggtatgctg accgtttaaa tgataaacgc
     2401 tgtgaaacgg ggttaaaaag gtggtaaaca cctgttcgat ttctgccacg ccgtgcattt
     2461 caaacaccaa ttttccgccg atgtaggtgt ttacgacggc atcttgcgtg aaaagcggca
     2521 tctgctcggc aacctttttt tcatcggcaa ggttagagaa tgtatctact aaatctttta
     2581 gtgctaaacg atcttggatt tgttgtaagt tcattgtgtt ttccttctga tttttgcaaa
     2641 ttttctttgg tttttcaccg cttgttaggc tgcaatacca acactttcac ggatttcgct
     2701 gttttcacgc aaaatgcgag ccacaaaatc ggcaatggat ttgcgggaaa cgctgtgtcc
     2761 tttgaacggt tcgcctttgt gagtcagctg atattgcact tccgaaccgt tggtaaacca
     2821 gcccgggcgg acaacggtat aatccaactc gctggcttca atcacggctg ccgattggcg
     2881 gtaaggctct aaaattgccc cgtgatcttc gccggtttcg ccataaatcc ccattgagct
     2941 aatccaaacc aagcgtttta cctgagcttc attcattgcg ttcaccacat ttcccgccat
     3001 cgcttttaag tcgccggcaa gtccagcgta aacggcatca acgccttgca ttgcttgggt
     3061 taatgcgtgg aaatccaaca catcgccttg cacaattttg acgcgttcag tttcaaattt
     3121 cgctactgat tgcggacgac gggcaaaaag ggttaattgc acattattgt cttttagcag
     3181 ttcaggaatc acgacattcg ctaggctacc gcttgctcct aaaattaaga tctgtttcat
     3241 tgctgttctc cattgaatga agtcagtata gcggtgtaga ttgcatttga ttagtagggt
     3301 aaaatcgctt gaagtaatga gaaaaattca ttaataaggt ggttaaatga aagaaaacct
     3361 caacgattta agagcttttt tgttggtagc acaaacgagc agttttacca aagcagcagc
     3421 acagatgggc gtgtcgcagt cggcgttgtc gcacagcatt cgggggattg aagaacggtt
     3481 gcaaatcaag ctgtttcatc gcaccactcg tagcatttca accaccgagg caggggagag
     3541 gctgtatcaa cggctgttac cgctgtttga tgaaatcgac caagaagtaa atgggctgag
     3601 tgagtttcgc aatgcgttaa aaggcgggct gagaattaac ggtaatgaac acgttttccg
     3661 tgatgtgctg gcagataaat tcgctgagtt tgtcaaacgt taccctgaag tagcattgga
     3721 gctagtggcg gaagatcgct ttgtagatat tgtcgccgag cgttttgatg ccggtattcg
     3781 gcttggggca gatgtggcga aagatatgat tgcactacgc atttcgcccg atgtgaaaat
     3841 gaccgcagct gccagcccca actatttggc acagcacggc acgccgaaaa cgccctttga
     3901 tttaaccgaa catcattgtc tcaaacatcg tcttgcgaca ttgggcggca ttctggcgtg
     3961 ggaatttcgg gatcccatca ccaagaaaat cgttaaattt caaccgcagg gcaatcttac
     4021 tgccaataac ggcttcttgc tgcaacacta tgccaaacaa ggtttaggca ttctttggag
     4081 tccgcaagac agcattcaaa ccgagctgga taacggcagc ctcgtgccga ttttaaccga
     4141 ctgggcaatg cagtatgagg gctatcatct ctattacccc aaccgtcgcc aaaattcgcc
     4201 tttgctgaaa gcgttaattg atgtgctgag aggataggta aaaatgagcg gtcgttttta
     4261 gcaaaaaatt gaccaaaaaa cgaccacttg tagagaagct aaattatttc acacgagaaa
     4321 catattcgcc tgaacgggta tccacacgga taacttcacc gatttgaacg aataaaggca
     4381 cgtttaccac agcaccggtg cttaatgttg ccggtttacc gcctgtacct gcagtatcgc
     4441 cttttaagcc cgggtcggtt tcgacgattt ctaactcaac gaagtttggt ggtgtgactg
     4501 caattgctga accgttccat aaagtcacga tacattctgc attatcaact aaccatttta
     4561 cgttgtcgcc taacgctttt tcatctaccg agatttgttc aaaggtttca gggtgcataa
     4621 agtaccagaa atcaccatcg ctgtaagagt agttgtagtt agaatctact acatctgctg
     4681 cttcaaccga agtgcctgat ttgaagttga tctctaatac tttgccagag attaatttac
     4741 ggattttagt acgggtaaat gcttgacctt tacccggttt tacgaattcg ttttcaacaa
     4801 ttacacaagg ttcgccatct tgaataaatt ttaaaccttt tttaaagtcg ttagtgctgt
     4861 agctagccat tatttcctca attatttaaa aaataacaat aaattaacaa attttgaacc
     4921 caaccctatt gggtttgtca aaaagagcca tataataacc taaattcaca aaattagcga
     4981 aagatctctt gcgatttttg ctataatttt tcaaacttta ttgataagga atgcgaatga
     5041 aaaaactgat tactctttct gcgattttag ccgtttctgt tgcaaccgtg gcagaggcaa
     5101 aacgtggcgg tgggagcttc cgttcagtta gcccgtcaaa agcggtagca actaagccgg
     5161 tagcttctca aaaatcaacc acaaatcagc aaacgcaaca aaaagatgct acttttgcca
     5221 atacgccaaa cagtgcaact gcccagccac aagtgcgtca aggcagcacg atggcaaatg
     5281 tagcgatggg ggcagcagcc ggttatattt taggtgatat gatgtcatca aatgcacatg
     5341 caaatcagcc tagccagcct gcggatgcaa atgcacaagc ggtcaattct gcacaaaatg
     5401 ttgcaaatgg agcagttcct gctattgcag aatttaagag tattggcggt caaatcgatc
     5461 cgtttttagt agaaaaaacc gatggttatc gccgttattg tatttcaggc gtgcaatacc
     5521 tagtcgctgc acaaggtgta caaacctcac caactgtaat ggttaatcca aatggcacgc
     5581 cattacaatg taacttaatt ccataattat ttttatttgc gggcaggcaa aaacctgccc
     5641 cttgtatcta caatattcaa tgcaaccaat tcaaatcaaa ccgctttggc taaccgagct
     5701 ggctcaagcc tttaataacc ctatcgactt attacaattt cttgaactca atccaaatga
     5761 ttttgagggc gatatagctg cccgtaaatt atttgctctt cgagtgccaa gaatgttcgc
     5821 cgaaaaaatg gaaaagggta acccaaacga tccgcttttt ttgcaagcaa tgtctttaca
     5881 ggcggaattt attgaggcag aagggtttgt ggtagatcca ttggaagagc aacaaagccc
     5941 agcaccgaat attttacata aataccataa ccgtttgtta tttatgctaa aaaacagttg
     6001 tgcgattaac tgccgttact gttttcgccg tcatttccct tatgaggaag tgaaaagcgg
     6061 taaagctgtg tggcaacagg gactaactta tattgctgag catcccgagc tggaggaagt
     6121 gattctgtcc ggtggtgatc cgctgatggc aaaagatcag gacttagatt ggatattaac
     6181 gcaattagag cagatttcgc atatcaaaac cctgcgtatt cattcacgct tgccggtcgt
     6241 tattcccaat cgaattacga cagaattatg tgaacggtta tcaaaatctc gcttgaatgt
     6301 cgtgttggtt acccatatta atcacgccaa tgaaattgat gctgtttttg caaacaaaat
     6361 ggctcaactg aaaaaagccg gcgttgtatt gctgaatcaa tcggtgatgt taaaaggtgt
     6421 aaacgataat gctcaaacct taaaaaggtt aagtgataaa ctttttgaat atgggatttt
     6481 gccttactat ttgcatttat ttgataaagt ggcaggggcg agccatttct atattgaaga
     6541 tgaacaggct ggtgagattt accgagaatt acagcgtatc acttcaggct atcttgtgcc
     6601 aaagctggca agagaaatag cgaaagagcc aaataaaatt ttgaaaggtt agcttaaaca
     6661 gaaatcaaga aacaaaaggc gaaaatggta aaattttcgc cttttttaac cgcttgttgc
     6721 aagcggttaa attctcttac ttttttgcaa attagaagtt cgcattgcgt ggagcacgtg
     6781 ggaatgggat tacatcacga atgttttgta acccggttac atacacgatt aaacgctcga
     6841 aacctaaacc gaaacctgag tgtggcactg taccgtattt acgcaaatca cgataccacc
     6901 agtaatcttc agggtttaag cccatttcat tcatacgctg atctaacact tctaaacgtt
     6961 cttcacgttg agaaccaccg ataatttcac cgattcccgg agctaaaacg tccattgcgg
     7021 caaccgtttt gccgtcatca tttaaacgca tatagaaggc tttaatgtct tttggatagt
     7081 ttttcacgac caccggcgat ttaaagtgtt cttcggctaa gaaacgctcg tgttcagagg
     7141 aaagatcgat tccccaagac accgggaatt caaattcttt gccggatttc aataagatct
     7201 caatcgcatc ggtgtaatct acttgagcaa atggcgaatt gataaagttt tctaaacgac
     7261 taatcgcttc tttatcaacg tgtttaacga aaaattccat atcatcttta cgttctgcaa
     7321 gcaccgcttt aaacacatat ttcagcatat cttcggctaa ttttgcattg tcgtttaagt
     7381 cggcaaaggc aacttccggc tccatcatcc agaattctgc aagatggcga gttgtgtttg
     7441 agttttctgc acggaatgtc ggaccgaaag tataaacttt acttaaggca cacgcatagg
     7501 tttccgcatt taactggccg gatacggtta agaacgattc tttaccgaag aaatcttggt
     7561 taaaatccac tttgccgttt tcgccacgag gtagattttc taaatccagc gtagaaacac
     7621 ggaacatttc acctgcacct tcagtatctg aggcggtaat tacaggtgtt gctacccaat
     7681 agaaaccttg ctcgtggaag aaacggtgga tagcttgagc taaacagtga cgaacacgag
     7741 caactgcacc gattaagttc gtacgagggc gtaagtgagc gacttcacgc agatattcga
     7801 tagagtggcg tttcgctgcc attgggtagg tatcaggatc ttccacccaa ccaagcactt
     7861 ccactttact tgcttgtaat tcaacggctt gaccttcagc aggggattcc acaactttac
     7921 cggtcaccac cacagagcaa cctgcggtta aatgtaatac ttcggcatta taatttggta
     7981 gatcgttatt aataatcgct tgaattgggt caaagcaaga accatcgtaa acgctcaaga
     8041 aagataaacc ggcttttgaa tcacggcggg tacgcaccca accacgcact gtcgcttctt
     8101 cacctaccgc tatcttgccg gataggattt ccgaaactgt tggaaattta gacattgaat
     8161 acctcaaatt ctttgttgtg agttttaata taagaaacgt tccgtattct acaagaaaat
     8221 agggggaaat ataataattt tacggaatta tttgcaaatt tgtgagtcta tataagcatt
     8281 gcaagtagtt aaatctgatt ttgcttggtg ttaaaaaatt ttttattttt taatgaa
//
LOCUS       DANLXQ010000109         8302 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.109, whole genome shotgun sequence.
ACCESSION   DANLXQ010000109 DANLXQ010000000
VERSION     DANLXQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.109"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..547)
                     /locus_tag="PXD94_001872"
     CDS             complement(<1..547)
                     /locus_tag="PXD94_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL6286708.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKISAETLNIQNNEVKALKDKTLEVLTLGAQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLENILRAKY"
     gene            complement(614..1198)
                     /locus_tag="PXD94_001873"
     CDS             complement(614..1198)
                     /locus_tag="PXD94_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL6286709.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
     gene            complement(1330..2514)
                     /locus_tag="PXD94_001874"
     CDS             complement(1330..2514)
                     /locus_tag="PXD94_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL6286710.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
     gene            complement(2597..3460)
                     /gene="rapZ"
                     /locus_tag="PXD94_001875"
     CDS             complement(2597..3460)
                     /gene="rapZ"
                     /locus_tag="PXD94_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL6286711.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWLPMLEQNNRSYLTIAIGCTGGKHRSIFIAEQLAKYFQAKGKNVQVRH
                     KSLEKHHKKTS"
     gene            complement(3473..3985)
                     /locus_tag="PXD94_001876"
     CDS             complement(3473..3985)
                     /locus_tag="PXD94_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL6286712.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            complement(4027..4752)
                     /gene="lptB"
                     /locus_tag="PXD94_001877"
     CDS             complement(4027..4752)
                     /gene="lptB"
                     /locus_tag="PXD94_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL6286713.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            complement(4764..5270)
                     /gene="lptA"
                     /locus_tag="PXD94_001878"
     CDS             complement(4764..5270)
                     /gene="lptA"
                     /locus_tag="PXD94_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL6286714.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAANVKITRQEGKKETIDATGSPVTFQQTLDNGKPVNGKG
                     NSVHYDLNSEFLTLIGNAELKQQGSFIKANKITYDVKKQQLKATSGGKTRVKTVLIPN
                     ELQDNKKK"
     gene            complement(5294..5878)
                     /gene="lptC"
                     /locus_tag="PXD94_001879"
     CDS             complement(5294..5878)
                     /gene="lptC"
                     /locus_tag="PXD94_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL6286715.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            complement(6032..6835)
                     /gene="xthA"
                     /locus_tag="PXD94_001880"
     CDS             complement(6032..6835)
                     /gene="xthA"
                     /locus_tag="PXD94_001880"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL6286716.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            complement(6933..>8302)
                     /locus_tag="PXD94_001881"
     CDS             complement(6933..>8302)
                     /locus_tag="PXD94_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL6286717.1"
                     /translation="NEVFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEGAGF
                     TEALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHSPSG
                     RSVTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVACALT
                     FGLTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMILGL
                     LFAFFVSYRKPRVYIENMPEPTADDIEARVANVKPFHIGISIVAIIATCSVQLATNST
                     IIGGLVGLVIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGGVPA
                     LVESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCISFGFSPLAT
                     MAIVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNIPLLVFGWI
                     AAMTL"
BASE COUNT         2306 a         1661 c         1675 g         2660 t
ORIGIN      
        1 catatttagc tcttaatata ttttctaact gttgcccttc ttcagctaat tgattcaatt
       61 ttgtttgtaa ctcgccaaat gctttatgtg cttccggtgt aggatttttc tccattttag
      121 caccttctac gatcatctga gcccctaaag tcaggacttc taaggttttg tcttttagtg
      181 ctttgacttc attattttga atattcaggg tttcagcact tatcttaata tgatcaattt
      241 gagctaataa tgcgttatta actgtctctt gcattaaggt agaatcggct ttttgtttgt
      301 cagtaagtgt ttctgttgcc gctttaattg catcatttaa ggctttttct tgagtgtctt
      361 gccattcccg taacatttta taatcttgag cgccggtatc agcaggttta acttcttctt
      421 ttgactcttc tttcacttga actggcgatt ctgctgagct ctgtgttttg gtagaattag
      481 gttgttcgca agcggttaaa aaaagggtaa ataatacaga taggctaatt ttagcgagcg
      541 tgttcatagt aatccttagt aattgcaaaa aaaagctaga gcaaaatact ctagcttttc
      601 attgaatggt aaattattta gctgagaatc tttgctgtaa ttctacgttt aatttttgta
      661 actcattacc agcttcaatt aaagactgag ctttttgttg tacggcttgt aatgctgctt
      721 ggtcatttgg tgtagagatc gtttttactt gttcagaaat cacttcactt gaaagtgtta
      781 aaactgattt tgttttttct ttaaatgttt tgatttgtgc atcagatact tcaatcgcat
      841 ctaagctttt tacagtttct tctacttttt tattaaatgc acttaatcct tcttgaattt
      901 gtttaggatc ttgtgtagct aatttctttt gtagttctgc ttgtgctgtt gccattgtgc
      961 tttcttgtgc cgtattccaa tcaactaatt tttggtaatc tgcttttgca gcttcgcttg
     1021 gagcggctac agctgtttca gcaggtttgg ctgcttctgc agcaggagct gttgtagttg
     1081 gtgcagcttc agttgttttt gtaggttctg ttgccggtgc tttttctgct ggtttatcac
     1141 aagctgctaa gaataatgcg actaaagcgg ttgcactgat ttttgttaat ttgttcatta
     1201 aaaaattcct caagaatata aaaataaaag ccaataaagg ctggttattg taccaataaa
     1261 tagacaagta atctccgtat tagttcataa aatcgttcat ctttacgtta tttcgaaaaa
     1321 atttacattt tataagtaaa tagcttgctg aataagctgc tttttcacaa aaggcagctg
     1381 gttggttagg cttaaaccaa ttcctctgaa taatttttta agcgggtgat tgccgtcaaa
     1441 tagctgtttt aatccttcca ttgtggctaa taatttcatc gcttgggctt tacgctcacg
     1501 ttcaaattgg cgtaaatggc ggtattcacc aatatcttgg ttgtttacca actgtttttg
     1561 gatttcgttt gccagcgttc tggcatcggc aaagcctaaa ttgacaccaa gtcccgccaa
     1621 tgggtgaatg gtatgagcgg catcaccaat caacgcaata cgaggttggg caaagtcacg
     1681 gcagaagcgt gctgcaagcg ggtaaatttc acgcaaactt tgcacttcac ataagccaag
     1741 ctgattatcg aaggcaatcg ttaaggcttt attaaattcg gcttcgcttg cattttttaa
     1801 ttcattggct ttgtttggtg gcagcgacca aacaatcgag cagagatgct catctgccat
     1861 tggcagaaaa gcaaaaatgc tatcttcagc aaagatttga cgagcggttt tgccgtgctt
     1921 ttcggttgtt ttgacattac agactaaggc ggtatgttga taatctttac tggtaagcgg
     1981 cattttcatc tggttacgca cccacgaatt tgcaccgtct gccccgacaa ccaatttagc
     2041 agacagcatt tcgccgttat ctaaggttaa aaatgccccg ttttcgctca cgcccagagt
     2101 ttggggggcg gcgataatat attctacatt agcttgttga gaaacctgtt gccacaatgc
     2161 gtgttgaatc accgcatttt ccaaaataaa gccaagttgt gaaagtccta attgcttgat
     2221 ttcattatca ttattatcaa aatggatatt agcaaagctg tctttttccc acactttcat
     2281 ttcggtgtaa ggagaaaggc gttgagccgg aatgtgttga aaagcaccaa tctgttccag
     2341 tattttttgg cttgaagcat taatcgcact tacacggtgg taaatcgtat cggcatcaaa
     2401 tttgggcgtg tttttctcaa taattttaat ttggcaatcg acatctttga gcagtccggc
     2461 aagagccagc ccaaccatac cgccgccaac aataacaata tctgcggatt tcataaaatc
     2521 aaatgtaggg gcggtgccct gtgcccgccc taaaatagtt gaaatgaggg ctggcacagg
     2581 accagcccct acttatttaa gatgtttttt tatggtgttt ttccaaactt ttatgccgaa
     2641 cttgcacatt tttgcctttt gcttggaaat attttgccag ttgctcggca ataaatatgg
     2701 aacgatgttt tccaccagtg caaccaatcg caatagttag gtagctacgg ttattttgtt
     2761 ccaacatcgg cagccacatt tctaaataat tgcgggtttg gtaaataaaa ttatgcactt
     2821 cggtttggcg ttctaagaaa tcaatcaccg gttgctctaa gcctgtcatt gggcgaagct
     2881 ccggattcca atgtgggttc ggtaaaaagc gaacatcaaa aacgtaatcg gaatcggcag
     2941 gtaagccgta tttaaaaccg aatgattgga aaataatttt aagctctttt tcggttgagc
     3001 cttgcagtat ttggcgaaga ttttccgcta attcgtgcga agaaagatga ctggtgtcaa
     3061 taatataatt tgcattttgc accaaaggct caagcaattt ttgttccaaa tccaatgcac
     3121 cttctaacga gagatcgtct tgggtggaaa gcgggtggat tcggcgggaa tcgctataac
     3181 ggcggattaa ggtattgcgt tcgcaatcta aaaaaatcag cttagcattt aaaggctcaa
     3241 gctgggtaag caattcttca atcgcattag gcgtattcgg taaattacga atatctaagc
     3301 tgatgactgc cgaacgattt tcttgagtaa ggtagtaggt taaggttgga attaaaggta
     3361 aagggatatt atccacgcag taataacctg catcttctaa tgctcttaat gctaccgatt
     3421 taccagagcc tgagcgtccg cttataatga ttaattccat atttcctcag tattattctt
     3481 catcttttag gtgattatca gcatacacca aaatttgcca aatatcttct gcggattcag
     3541 ctgctcggag ctgttttaat aacgatttat cggtaagttt ctgtgcaatt gcaggtaggc
     3601 aacttttata ctgttcgcag ctattttccg gaaacatcat tgcataaatt aaatccactt
     3661 ctttgttgtc ttgggcttcg tagtcaatcg cttcttccag ttgtaaaaag accgcaatcg
     3721 gtttagtcag ctcaatatca ccgcaagcgg gcaattttgc atgaggaagt gcaatgcctt
     3781 gatttaagcc ggttgtgcct aacttttcac gtttaaataa attgccaaag cattcaatag
     3841 ggcagagtgc ttcagattgc tgttcggttt ggttgattga ttcagccacg attttaccca
     3901 cgagttctaa cgctcgtttt ttacttgaaa tcagtacacc ttggcgaaca ttttcagggc
     3961 ttagaaattc ggttagtttt gtcataattt ttcatctaaa gatgaaatcc ccaaagaggg
     4021 gattttttag agtttgaatt gatcgcctaa ataaacacgt tttacatcag ggttttcaag
     4081 cacttgctgt ggtgtgccgc tggcaatcat ttcgccgcta ccgacaatgt aggctctttc
     4141 acatacatct agagtttcac gtacgttatg gtcggtaatc aataccccta aaccacgctc
     4201 ttttaaattg acgataattt ttttaatatc aattaccgag attgggtcaa ccccagcaaa
     4261 aggttcatct aacagaataa atttcgggtt cgctgctaat gctcgggcaa tttccacacg
     4321 gcggcgttca ccacctgaaa gtgattgacc taaactattg cgaatatgct caatatggaa
     4381 ttctgtaatc agctcttcgg cacgctcttt acgctgttta tcattgatat ttttacgcac
     4441 ttgtaatacc gacattaagt tatcgtacac acttaaacgg cggaaaatag atgcttcttg
     4501 cggtagatag ccaatccctt gttttgctct gtcgtgcatt ggtagtgtgc tgatatcttg
     4561 gtcgtcaata cgaattgtgc cgtggtcgtg gcgaaccaaa cccacaacca tatagaaagt
     4621 ggtggtttta cctgcgccgt tcggaccgag caagcccacg atttcacctg atttgacatt
     4681 caggctgacg tcttttacca cttggcgttg tttatagctt tttgctaaat gttcggcata
     4741 aagcacagac atatcattta accttatttt ttcttgttat cttgaagttc atttggaatt
     4801 agcacggttt tcactcgggt tttaccgcca ctggtagctt taagttgttg ttttttcaca
     4861 tcataggtaa ttttattggc tttaataaag ctaccttgtt gtttgagttc cgcattgcca
     4921 atcagcgtta aaaattcaga gtttaaatca taatgaacgc tgttgccttt accgtttacc
     4981 ggtttaccgt tatctaaggt ttgttggaat gttacaggtg agcctgtagc gtcaatagtt
     5041 tcttttttac cttcttgacg ggtaattttg acgtttgctg cggtcacttt aatcgaccct
     5101 tgagtgataa cgacattatc gctaaaggtc acgatattgt tagtcatatc taacgattgg
     5161 ctacctgaat caatattaat cggctgatcg gtatcgcctt ttagagcata agcggaaaag
     5221 ctgctaccca gtaacacgct cactaaaata gatttaaaca ttaatttcat tgttattcct
     5281 tgttccaagc aaattattct ttagtttctt gaataatggt cggttcaatg taagatttta
     5341 catctttttg caaagtcgca acttgttgtt ttaagttacc ttttaatcca acccccgttg
     5401 tagtaaaacc ggaacctatc gatttgaccg tactttcggt aaaaatatcc tgtgtattta
     5461 aatcaacagt taacgtatcg gtagtgattt gttgcaaacg tgatgcagga tctaatgcaa
     5521 caagtttgat attacctttt aaatttaaca ttttttcttt ggtaatttct gcataatcgg
     5581 cactgacttt ccactgtttt tgcgtcgact ctttggcaaa aagctcaata aaaggtttaa
     5641 aaaactcagt acgctcagta gactcatagc gtttaatttc agtagcttga gcgaaatatt
     5701 gtgggtttcc ttttagatca tagaccgtgg tggacatttt attccccaca tattcaggct
     5761 gtccttcctt tttaataagc tgatcaagcc ccgtattctc tttctcttgt aagctgtaat
     5821 accagccacc aagtacggca acaataatca gtaaaataac ggttaaacgt ttgttcataa
     5881 tccaaaaata atctaaaaat agtgtttgat tgtagcacag atttgaatag ggatatagta
     5941 cagcgtaaaa agtgcaaaaa agtgagaaaa aataaccgct tgtagggcta aaaactgaca
     6001 agcggttgaa aactcagatt tttttgcaac tttatttaaa ggttgcccaa acaggggagt
     6061 ggtcagacgg tttttccatt gctcgaatgt ctaaatcaat gcctgtttcc acgcaacgct
     6121 cagcaagtga ttttgaagca agaattaaat caatacgcaa gccacgatta tcatcaaaac
     6181 cttttgaacg gtagtcaaac caagagaact tatcgttcac gtctgggttg cctgcacgga
     6241 aagtatcaac caagccgtag ctgtataagc gatctagcca ttcacgctct tcgggtaaga
     6301 atgaacattt accgtcacgc agccagcgtt tgcggtttgg ctcgccgatg ccgatatcca
     6361 aatcagtcgg gctgatgttc atatcgccca taataataat cggattttcc ggctttaatt
     6421 cgctttccaa gtagttctgc aaatcagcat agaatttttg ttttgccggg aatttggttt
     6481 cgtggctgcg attttccccc tgtgggaaat agccgtttaa tacggttagc acgccgaaat
     6541 cggtctcgat atccgccata atcattcgtt tttgagcatc aagtacatct gtcgggaagc
     6601 ctttacgcac cgctataggc tcttttttag tcagtaatgc cacgccgtaa tgacctttct
     6661 gcccgtggtg gaaaacgtgg tagcctaagt gattgactaa atcgtaagga aaatcttcat
     6721 cggcaacttt gatttcttgt aagccgagta catcgggctg gtgtttttcg ataatcgctt
     6781 cgagctgatg tggtctggct cgtaatccgt tgatattaaa agagataaat ttcattggtg
     6841 atcctaatgt ggtttgcaaa ataaagaaga aaatagaccg cttgtaatgg ctacaagcgg
     6901 tcatttttag caaaaatttt gcatattata gcttaaagtg tcattgctgc aatccagccg
     6961 aacactaata atggaatgtt aaagtggata aaagttggta caacagaatc ccaaatgtgg
     7021 tcgtgtttac cgtcagcatt caagccggag gttggaccaa gtgtggaatc tgatgccgga
     7081 gaacctgcat caccgagtgc agcagcaaca ccgacaatcg ccattgtcgc cagtggcgag
     7141 aagccaaatg agatacaaag cggcacataa attgaggtaa tgatcggcac agtcgagaat
     7201 gaagagccga tacccatcgt aataaataag ccaacgacta acattagcaa tgctgccatt
     7261 tcttttgatg aaatcacatc acgcaggctt tctaccaatg ccggcacacc gcctgttgaa
     7321 ttgaccacgc cggcaaaacc ggaggcggca atcatcacaa agccaatcat cgccattaaa
     7381 cgtaagccag cttggaagac atcgttactc tctttcagtc taaatacacc gcctaaggcg
     7441 aaaataacca gaccgactaa gccgccgata atagtcgaat ttgttgctaa ttgcaccgaa
     7501 caggtcgcga taatcgccac aatactaatg ccgatatgga agggcttaac gtttgcaaca
     7561 cgggcttcaa tatcatctgc ggttggttca ggcatatttt caatatacac acgcggttta
     7621 cggtatgaca caaagaatgc aaacagtaag cctaaaatca taccggaaac aggaattaac
     7681 atcgctttgg tcatttccgc tgtggtagtc actaattgga acggttcacc tgcttggttg
     7741 atatttttaa ctaagatgct ttcaataaag atctgaccga aacctgccgg aattaacata
     7801 taagtggcgg ttaaaccaaa ggttaaagca caagctaccg cacggcggtc aatttgcaat
     7861 ttattcatta cgcttaatag cggtggaatt aaaatcggaa taaaggcaat atgaattgga
     7921 attaggtttt gagatgacat tgcaaatgcc actaacacaa ataatacaat gtatttaaac
     7981 caagtaacgg agcgacctga cgggctgtga ccaaaggatt tgatcacttt ataggctaat
     8041 aaatcggtca cgcctgattt tgatagagct acggcaaacg caccaagaac ggcatagttc
     8101 atagcagttt ccgcaccccc acctaaacca ccagtaaaac tttttattgt tttttccaat
     8161 gcttcggtaa atccagcccc ttctacacca agattaccga ttagaccgca agtaagggca
     8221 gaaataataa gtgcgattac tacattaatt ctaagcaagc ttaggattaa cagcacgata
     8281 atcgaaataa agacttcgtt tg
//
LOCUS       DANLXQ010000110         8265 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.110, whole genome shotgun sequence.
ACCESSION   DANLXQ010000110 DANLXQ010000000
VERSION     DANLXQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.110"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(16..645)
                     /locus_tag="PXD94_001882"
     CDS             complement(16..645)
                     /locus_tag="PXD94_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL6286718.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            749..1396
                     /gene="udk"
                     /locus_tag="PXD94_001883"
     CDS             749..1396
                     /gene="udk"
                     /locus_tag="PXD94_001883"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL6286719.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            1411..1992
                     /gene="dcd"
                     /locus_tag="PXD94_001884"
     CDS             1411..1992
                     /gene="dcd"
                     /locus_tag="PXD94_001884"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL6286720.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
     gene            2070..2225
                     /locus_tag="PXD94_001885"
     CDS             2070..2225
                     /locus_tag="PXD94_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDL6286721.1"
                     /translation="MLHYAVVFFVIAIIAALLGFGGIAGSATEIAKILFFVFLILTVI
                     SFFFKKK"
     gene            complement(2354..2956)
                     /locus_tag="PXD94_001886"
     CDS             complement(2354..2956)
                     /locus_tag="PXD94_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL6286722.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGWKWYKNKKQYKK"
     gene            complement(2958..3977)
                     /gene="purR"
                     /locus_tag="PXD94_001887"
     CDS             complement(2958..3977)
                     /gene="purR"
                     /locus_tag="PXD94_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL6286723.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
     gene            complement(4113..4841)
                     /gene="cmoA"
                     /locus_tag="PXD94_001888"
     CDS             complement(4113..4841)
                     /gene="cmoA"
                     /locus_tag="PXD94_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL6286724.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNSNGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(4851..5633)
                     /locus_tag="PXD94_001889"
     CDS             complement(4851..5633)
                     /locus_tag="PXD94_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL6286725.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTGTVVRAINKFLEK"
     gene            5752..6318
                     /gene="seqA"
                     /locus_tag="PXD94_001890"
     CDS             5752..6318
                     /gene="seqA"
                     /locus_tag="PXD94_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL6286726.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            6328..7746
                     /gene="menE"
                     /locus_tag="PXD94_001891"
     CDS             6328..7746
                     /gene="menE"
                     /locus_tag="PXD94_001891"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL6286727.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQTASQ
                     RQAVLDDNGVELIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEDKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            7739..>8265
                     /locus_tag="PXD94_001892"
     CDS             7739..>8265
                     /locus_tag="PXD94_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL6286728.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQVVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTSTQSQLETVQHDLSIINNNLVAQNTAPDKAQTALTEMVKR
                     KQEISSQLSATNIKN"
BASE COUNT         2712 a         1665 c         1422 g         2466 t
ORIGIN      
        1 aaaatttaaa ctccactaga aaatttgcag agcaagttga taagtataaa tcagtaaact
       61 aaaagcgata aagtacataa ttagcccggt aacagcatct aaaatttgcc aagttcttcg
      121 ctttgcaaaa taaggggaaa gtaagcttgc tccataacct atgccaaaaa accaaataaa
      181 tgagcaaagt aatgcacctg ctaaaaagta tattttaccg ttaaaatcta atttcccacc
      241 gatactgcct acaatcacaa ccgtatcaat ataaacgtga ggatttaaga atgtaatcgc
      301 aaaggtaatc agtaaagcct tttttaagct cgttgcattc tctccacttg ccacaaggta
      361 ttcaccactt ttaaatacgc tgataaatga acggctaccg tagcttaata gaaaaatggc
      421 ccctaaaagc gaaataatga aacttacaac aggcaattcc gcaattaacg tgcctaagcc
      481 tagcacacct atcgtcatta aaaatacatc acctaaaaaa cagagcaatg caatccaaaa
      541 tatgtgttgt tttaaaatcc cttgctttaa taaaaaggca ttttgtgcgc caatagagac
      601 aattaagcca aaacacacta taaatccttg aatgaaaata tccatttttt ctccaaaatc
      661 gcataacaag cggtcaatta tacaaaaaat tttgcaaaac ttcggtataa tttatcgaat
      721 actcttttga ctgaatagga aattttttat gactgatact agccagactt catcttgcgt
      781 tgtcattgcg attgccggcg catctgcctc aggtaagagc ctgattgcct caaccatcta
      841 taaagaactc aaaaatgaat tgggaacaga taatatcggt attatttccg aagatgctta
      901 ttatagagat caatctcatc taacaatgga agaacggatt aaaaccaatt acgatcaccc
      961 taattcaatg gatcatcatt tattggttga acatttacgc caattaaaac aaggtaatgc
     1021 gattgaaatt ccagaatatg actataccga acataatcgt aaaacgacca cgaagcactt
     1081 tgaaccaaag aaaatcatta ttttagaagg tattttattg ctgacagatg agaatattcg
     1141 caatgaaatt aatgtttcta ttttcgtaga tgctcctctg gacatctgct ttatccgccg
     1201 cttacaacgt gatatggtgg aacgtggacg ttcgatggaa tcagtgatta gtcaatatcg
     1261 caaaaccgtt cgcccaatgt tcttacaatt tatcgaaccc tctaaacaat atgctgatat
     1321 tattgtgcca aaaggtggca aaaaccgtat tgccattgat attcttaaag cacaaattaa
     1381 gcaattacta aaataaaata ggagctaaat atgcgtttat gtgatacaga tattaaacgt
     1441 tacttagatg aaggtattat tgccataacg cctcgacctt cagacgataa aatttctggc
     1501 gccactgcag atgtgcgttt aggtaattcg tttcgtgtat ttcgtgaaca taaaacgccc
     1561 tatattgatt taagtggccc tagagaagaa gttgccgctc agttaaataa agtgatgagt
     1621 gatgaaatta tcatcggtga tgatgaagca tttttccttc atcccggtga ccttgcactc
     1681 gcaacaaccc ttgaatcggt ttccttacca gccaatatcg ttggatggtt agatggtcgt
     1741 tcgtcacttg ctagattagg cttaatggta cacgttaccg cccacagaat cgatccgggt
     1801 tgggaaggca aaattgtatt ggaatttttt aatgctggaa aattacccct agctttacgc
     1861 cctaatatgg caattggcgc attaagcttt gagattttaa gtggtgatgc tgcaaaaccc
     1921 tataatgcgc gaaaagatgc aaaatataag aaccagcaaa gtgccatttc aagtcggatt
     1981 aatcaagatt aatatagtac atgccttgca atcatacatt aactatgtta gttttttaat
     2041 cagtttatta cacagtaagg agtataacta tgttacatta tgcagtcgta ttttttgtca
     2101 tcgctataat cgcagcgcta ttaggatttg gtggtattgc tggaagtgct actgaaattg
     2161 ccaaaattct cttttttgtc tttttaatac ttactgttat ttcgttcttc ttcaagaaaa
     2221 agtgacacag ttaaacctaa ttttttaagt taaaatatag ttcaaaaaaa cagttggtaa
     2281 tggatattta taattattct taccaactgt tttgctatta tatcttttta agatgaaatg
     2341 ctcgtctttt gtttcacttt ttatactgct ttttattttt ataccatttc cagcctaaaa
     2401 atagaataac aatgcctaaa atggcaaaaa tgaggtgctg cccattagct acttgttctt
     2461 ttagccaatc caaattagat gccccatagt cgcctaaata aacccaaatt ggcacagaga
     2521 taatagcggc acagaaatca actaataaaa attttacgaa gctgactttt ctggtaatac
     2581 ctgaaactaa gtaaaccacc gcgcgcaagc caggtaaaaa acgagccata aaaagtagtt
     2641 tccatccttg tttttcaaaa cgttcttgca cggttgcaaa acgttcaggt gtcgcaacat
     2701 tcctgattaa tggaaatttg agaatttttt cgccatagat tctacctagc cagtacattg
     2761 tgctatcgcc aactaatact cctaacatac taacaaccag catccaatgc acattggtgt
     2821 agcctaatcc tgaaataaca ccaccggata ctaacgtaat atcttcagga atcggcacac
     2881 cgaaaccaca ggcaagtagc acaagaaaaa cagcccaata accatagcta ctaaaaaatt
     2941 caatcaatgt ttccatatta ctccttaatg tttaaattcc gaaccgattt acgtttgacc
     3001 aattcaggaa agaactctaa tactttaggc tcttttgcgt tttcgttatc ttcaatacgt
     3061 tctaataata attctaatgc tgccgagcct aagcgagatt ttgactgatg gattgtcgta
     3121 agaggcggtg cataaaagcg tgagctatgc acgttatcat acccgatcac tgaaatatct
     3181 tgcggcacat tcagcccttt ttcggtaata gctgaaattg cccctagtgc cattgagtca
     3241 ttaaaacaaa aaaccgcggt tggtaatttt tcactgctct taagcaaatt attcatacac
     3301 tcataccccc cctcaggttc aaaatcgcct tcttgaatcc attcttcacg aatagacaaa
     3361 cccgcctcag atagcgcttt tttaaaacct tcaaaacggg ttcttgcaat ttctttatct
     3421 ctattgccgg taattacagc aatatcttga tgcccgtttt caattaaatg ctgagtcgca
     3481 atatagccac cttcaacacc gtgatctaaa ataatatcac tgcgttcatc acttcgtccc
     3541 caatccataa tcaccattgg gatattgctg ttttcaaaga tatcaaatga attagctgta
     3601 tattcggagc acatcaccaa aatgccatca acccgttttt tgatgagcat atctaaatga
     3661 ttttgaattt tctcaagttt gttttgagta ttgcacaaaa atagtgaata ccctttgcta
     3721 tagcaaaatt cctctaccgc taacacaatt tcagcgaaaa acggtgattc gctggttgta
     3781 atgatcatac cgatagattt agtcgtatta attttcaaac tccgcgctac agcactagga
     3841 gaatagttca gcgctttaat agcctcccaa actgcttttg tagtgtcctc tgctacaaag
     3901 cgggttttgt ttataacgtg agaaaccgtt gtagtcgata caccagcata ttttgcgaca
     3961 tcttttatcg ttgccatacc tttttcctcc taaataagta gtggtgcatt ataactgatt
     4021 ctaactgact tttattataa agaaatagat agaatgcaag cggttgcttt ttatcaagaa
     4081 tttgcaaaaa attttacaaa tatcaccgct tgttaggctt taattgcaat cattgagcca
     4141 aaattaaagc attgaaacca aagttcaact tgtgtaaaac cagcttcttg cagacgtttt
     4201 ttatgggttt caatactatc tgttcgcatc acattttcca aagcattacg cttttggctg
     4261 acttccaatt cactgtagcc atttgctcgt ttgaatgtgt gatgaaggtc gatcaatagc
     4321 tcgttcattt cctcactctc aaaggtaaat ttttccgata acactaaaat gccattggaa
     4381 tttaatcctt gataaatctt ctgtaataat gctaggcgat cttctcttgg taaaaattgc
     4441 aaggtgaaat taagtaccac cattgaagca tttttaattt caatgtggcg aatatcatca
     4501 agtaaaatcg tcaccgggat ctcagagtga taagcactta aatgggtttt acaacgctca
     4561 accatcggct gagaattatc taccccgata atctgcacat tcttcgcatt cacattacgg
     4621 cgaatagata aaatccctgc gcctcgtgaa caacctaaat cataaacatt tgaattatct
     4681 gtgacaaaac gctccgccaa cattccaata gcagtaataa tattggaata gcccggcacg
     4741 gagcgttgga tcatatccgg aaacacttca gcaaccgatt catcaaaagt aaaatcgccc
     4801 aatttctcaa tcggagcgga aaataatgta tctctttgca tttttctccc ttatttttct
     4861 aaaaatttat tgattgctct cacaacagtt cctgtttttt ctgcgtgaac ccaatgatca
     4921 gcgtttgcca caatgaaaga tttggcctgc ggaaattgtg ccaaaatcgt ttgtgtatgc
     4981 tctgcttgaa tataatccga taatgcacct ttaataaaca aggtcggttt atccacaaat
     5041 acctgctgcc accccatcac attctgataa ttatctttta gcgcctgtaa attaaaacgg
     5101 aaagattgct cgctttgggc atcaaaagat ttcaacataa actgttgcac gccttcatct
     5161 cgaatatatt cagccattat cgctttagct tgctgacgat tctggggttt agcttctttc
     5221 accgcaaata aggctgccat aacctgctga tgccgatttt gggtataagt gaccggggca
     5281 atatcaatca ctaccaattt ctccaccagt tcaggagcaa atgcagccaa tgccatcgca
     5341 ctttttccgc ccattgaatg cccaaccaca atagcatttt ggatatttaa ttctgtgagt
     5401 aattgtgcta aatcttgtgc aatcaaagca taattcattt cgtctgaatg aaaagatcgc
     5461 ccgtgatttc tgagatcaac cattaaaatg gagtaacgtt ctgcgaatgg ttttgctatt
     5521 gcgcctaggt tatttaaatc tccaaacaat ccatgtaaga agatcatcgt ctgagcatta
     5581 gcatgattat tcgaaggttg gtaattaaag tttaactgcg atttttctgt catataaatg
     5641 tcattgttca tataaatttt cagcgatgtt cctctataaa aaacaataga aatgcgtata
     5701 atagcaagca ctattaacgc atttaatacc cgcagatatt tgaggatccc gatgaaacga
     5761 atcgaaattg atgatgaatt atatcagtat attgcaagcc gtacacaatc tattggtgaa
     5821 actgcgtcag atatattacg ccgtctatta cgcctgccac aatcgcctca gccttttgtt
     5881 ttagtacaag aacacatgat caacgagctt aaagagcttg tcaaaacgcc ttctcgtgct
     5941 acgaaaaaaa atgaatccaa tgcagaaaaa accgttgaaa aattggaaaa cattttaaat
     6001 tccgagcatt ttatgaacga aaataaaaat gtagtgcgtt ttactatgct attagccgca
     6061 ttatatcgag caaatccgga ggcttttgct aaagcaaccg aaaaagtgcg tggtaatgaa
     6121 cgtatctact tttcgcaaag tgaagccgaa attttagcta ctgggagtgg agtaaaagcc
     6181 aagcagatcc cagattcacc gttttgggta attaccaata ataataccgc acgcaaggga
     6241 cttattctca aaggcgtaat ggaatccatg aaagtaccaa gccagttaat cgaacgcatt
     6301 caagcgcttt ttatctaatt aattgaaatg ctcatcaatt ctactcgctt tcctacccaa
     6361 ttttgggctg ctcattcagc ccaaaatgtg gcgattattt gggaaaaagg aacatcagac
     6421 tatcttaacc accttccatc aaacatcact tggtatagtt tgaatcagtt gattgcgcaa
     6481 gctattttcg tgttgcaaca acaaggtgct actccgcaca gtttgattgc ctattgtggt
     6541 tctcatcggc tcattggctt gctcacttat tgtgctagcc ttgctttggg ggctaagatt
     6601 ctaatgctta accctgctca aacagcaagt caacgacaag cagtcttaga tgacaatggt
     6661 gttgagctca ttattaatga tgccgacttt gcaaaatttt cagcaaaaac gaccgcttgt
     6721 aatgccttcc ctacgattga ttttgaaaaa ccagctaccc taaccctaac ctcaggctca
     6781 agcggtaaac ctaaagcagt ggtgcattct atagcggccc atctctatag tgccgaagga
     6841 gtatgtgaat taatggcatt tgagcagcca aactcttggc tactgagctt gcctctgttt
     6901 cacgtttccg gacaaggcat tatttggcgt tggttattta aaggtgcaac actgtatgtt
     6961 cacgaagaca aaagtgattt ttttctttca gcacaaaaag tgagccatct ctctttagtc
     7021 cccacccaat tacaacgcta tttaaaccaa ttagattcta tggtcagcca aaaatgttta
     7081 ctgggtggat ctatgcttcc agccgaattg attgctcaag cacaacaatc tgggatgact
     7141 accttttcag gctacggaat gactgagatg gcatcaacca tttgtgcagt agaaaatgat
     7201 ttaaacagtg ttggttttcc gttaaaacat cgggaactaa aattagaaaa tggtgaaatt
     7261 tgggtaaaag gcaaatcact cggcttaggc tattggcaaa aaaatgggca aattcgaccg
     7321 cttgtaaaca cacaaggctg gtttgccact aaagatcgtg gcgaatggaa tcacaaaaaa
     7381 cagcttgtaa taaaaggacg attggataat atgtttattt caggagggga aaatatccaa
     7441 cctgaggaaa ttgaacaact gatttttcgt tctcagcttg tcgaacagat tatcgtgttg
     7501 ccgattgaag ataagcaatt tggacatcga ccagtggcgt tcttacaatt caagcaatct
     7561 gattcaaaaa atgaactcca taaattaaaa atttggctgt ctgataaatt agaaagattc
     7621 aaacagcctg ttgcctatta tttactggat atagaaaaat accaaaaaca aggcttaatt
     7681 aaactatcaa gaacacaatt acaacaagat ttaaaactca aagtattaaa ggaaatctat
     7741 gtttagaccg attttaaaaa catttgtgct tagtttaggc ttaatgctaa atagttatgc
     7801 attagctgaa ttgccaaccc aacaggtagt gcaaacgcag ttagatagcg ccaaaaaatt
     7861 agatcaagcc gatgcggata ataaggcatt ggttcaaaca cttgaagata cgcttgcttt
     7921 cttagcccaa attgataaac aaaaagcaga taatcaggca ttgaataaat ctattcaaga
     7981 ttcccaaaaa gcattaaaaa caagccaaca taatcttgaa aaattaaaag aggtgactat
     8041 tgcaagtgtc gataatttaa cacaaaaagc gacaacagat ttacaaaaag agttaacctc
     8101 tacacaatct cagctggaaa cagtacaaca cgatttaagt attattaata ataatttagt
     8161 tgcacaaaat accgcaccgg ataaggctca aacggcctta accgagatgg ttaaacgtaa
     8221 acaggaaatt agtagccaac tttcagcgac taacattaaa aatga
//
LOCUS       DANLXQ010000111         8094 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.111, whole genome shotgun sequence.
ACCESSION   DANLXQ010000111 DANLXQ010000000
VERSION     DANLXQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.111"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1092
                     /gene="rnd"
                     /locus_tag="PXD94_001893"
     CDS             <1..1092
                     /gene="rnd"
                     /locus_tag="PXD94_001893"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL6286729.1"
                     /translation="VETNEQLEAVCEQARKTKVVALDTEFIRTRTYYPILGLIQLFDG
                     QQVSLIDPNTISDFSPFVSLLADKNIIKVLHACSEDLEVFEYQFKQLPEPMLDTQVMA
                     AFAGIGISMGFAKLVSHYLNIELDKGASRTDWLARPLSAEQLQYAAADVWYLLPVYEK
                     LAQALTKSTWQSAVAEECVALLKKAKQPINKEKAYKNIANAWRLNREELAILQVLAKW
                     RIEEAQKRNLALNFVVKEASLVQIAKSCPKHTSSLLEFMHPNEVRIHGKKILWLVEQG
                     KAIESNDYPELIQRLVDEKGYKYYMQAMLQKLLEIQPLDIVPELVASKRQLNQLFKWY
                     IDGKPSEKIPELLIGWRKPFGEQLLSVLS"
     gene            1149..1631
                     /gene="lrp"
                     /locus_tag="PXD94_001894"
     CDS             1149..1631
                     /gene="lrp"
                     /locus_tag="PXD94_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL6286730.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMVSYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            1642..4302
                     /locus_tag="PXD94_001895"
     CDS             1642..4302
                     /locus_tag="PXD94_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL6286731.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNISGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     IQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     GKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            4454..5293
                     /locus_tag="PXD94_001896"
     CDS             4454..5293
                     /locus_tag="PXD94_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL6286732.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKGLTTGNPAVCN
                     TFN"
     gene            5382..6464
                     /gene="aroG"
                     /locus_tag="PXD94_001897"
     CDS             5382..6464
                     /gene="aroG"
                     /locus_tag="PXD94_001897"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL6286733.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAEAVEKRRNFAK"
     gene            6551..7243
                     /gene="tsaB"
                     /locus_tag="PXD94_001898"
     CDS             6551..7243
                     /gene="tsaB"
                     /locus_tag="PXD94_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL6286734.1"
                     /translation="MKPSILALDTATEACSVALQYNGNITFLDEISPRSHTQRILPMV
                     DELLNQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLPVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGEEWNEVVAEQVCSPEVAISQFQMSEET
                     VVVGTGWAAYSQFSEQNLPLVISDITLPSSRYMLSIAETEIQKGNIQSAFEIEPVYLR
                     NEVTWEKLPHKQ"
     gene            complement(7260..8069)
                     /locus_tag="PXD94_001899"
     CDS             complement(7260..8069)
                     /locus_tag="PXD94_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL6286735.1"
                     /translation="MTMTVIIILIICGIIVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKAIVPPKPKEAVSNATMCSLGGLIAGITGIGGGS
                     IMAPIVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYVNFA
                     IVGTVVISSLFTVYFSMKLRNILSPELTTKLLGVILFTIACYMGFLQYLFT"
BASE COUNT         2553 a         1475 c         1813 g         2253 t
ORIGIN      
        1 gttgaaacaa atgagcaatt agaggccgta tgtgaacagg ccagaaaaac aaaagtagtt
       61 gccttagata cagagtttat ccgtacacgt acctattatc cgatattggg tttaattcaa
      121 ctctttgatg gccaacaggt tagccttatt gatccaaata cgatttctga cttctcgcct
      181 tttgtgtcat tattagccga taaaaatatc ataaaagtat tacatgcctg tagtgaggat
      241 ttagaagttt ttgagtatca atttaagcaa ttgccagagc cgatgttgga tactcaggtg
      301 atggcagcct ttgccggcat tggtatttcg atggggtttg caaaattggt ttcccactac
      361 ttaaatattg aactagacaa aggcgcttcc cgaaccgatt ggcttgccag accgctaagc
      421 gctgaacaat tacaatatgc ggcagctgat gtttggtatt tattgccagt ttatgagaaa
      481 ttggcacaag cattaacaaa aagcacttgg caaagtgctg tagcagagga gtgtgtcgct
      541 ttgttgaaga aagcgaaaca gcctatcaat aaggaaaaag cctataaaaa tattgccaat
      601 gcttggcgat tgaatcggga agagttagcc atattacaag tattagcgaa atggcggatt
      661 gaagaagcac aaaagcgcaa tcttgcctta aattttgtcg tgaaggaggc aagtttagtt
      721 caaatcgcaa aaagctgtcc taaacacact tcttcgctgt tagaatttat gcacccaaat
      781 gaagtcagaa ttcacggaaa aaaaatacta tggttggttg agcaaggaaa agcaatagaa
      841 tcgaatgatt atcctgaact cattcagcgc ttggtggatg aaaagggtta taaatattat
      901 atgcaagcga tgttgcaaaa attattagaa attcaaccgc ttgatatcgt accggagttg
      961 gttgcaagta aacgtcaatt aaatcagcta tttaaatggt atattgatgg aaaaccatca
     1021 gaaaaaattc ctgaattatt aatcggttgg cgaaaacctt ttggtgaaca gctgctatcg
     1081 gttttatctt aaatttcatt cgggttacat agactatgta cagtataatt gcctaaaaag
     1141 gagtttatat ggaaaataaa aaaccaccaa aagcattaga tgcgattgat ttaaaaatct
     1201 taaacgagct acaacgtaac gggaaaattt ctaatattga attatcaaag cgagttgggc
     1261 tttctccaac gccctgctta gaacgggtaa aacggctaga aaaaaataac gtaattatgg
     1321 gctataaagc tttacttaac cctgaattac ttgatgcccc tttactggtg attgttgaaa
     1381 ttactttggt gcgtggtaag cctgatgtat ttgaagaatt taataaagcg attcaacaac
     1441 ttgatgaaat tcaagaatgc cacctggtat cgggggattt tgattatctg ttaaaaaccc
     1501 gtgtggcgga tatggtatcg taccgtaagt tattaggcac aacattgctt cgtttgcccg
     1561 gcgtaaatga cactcgaacc tatgtggtga tggaagaagt aaaacaaacc aactatttgt
     1621 tgttaaagta aggagaattg ggtgattgaa caacttaaac cacacctaaa aggcaaagag
     1681 aacttgatca aacttatgct tttgttgacc ggctttttgg gcgtttattt gctaattgct
     1741 tgggcaagtt atagtccatt agataatgct tggtctgttg caagcagcat tacacaaaca
     1801 accttgaata aaacaggtca ctttggtgct tggagtattg atatgctcta tgcaatgttt
     1861 ggtaaggttg cagttttagt acctattgca ttaattattt tctcaattta tagcttggga
     1921 atgggtttaa gtgtcgatat gaaatggaaa actgtcttca ttcgcctagc cagtttctta
     1981 ttattgatgg tcggtcttgc cgggttattt agcgtgcttt tttcaaactc agcttattat
     2041 ctgtcgggtg gctttgtcgg tggagtttgg gagacaatat tatctgatac tatcggacaa
     2101 tttggtgcct tattgattgc aatgcttttt actatagctg gcttatattt ttgctctgtt
     2161 caatcattat tgccaatatt aacgcaattt tatgagtgga taatggcaaa atcagaagag
     2221 agcaaaacag ctgaatctct tcagcatttg caaaatactg agcaaaaaca accgcttgta
     2281 actgaaaatg aagcggtgga agttgtagat gaccataaac cgcaatttac cgatatctcc
     2341 gcctttaaac gcccaaacat tagtggttta agacactcgg aaaatgtaga gatagataac
     2401 cgagaaatgt atgaaattga aagagatctt gagctaccga ctattaatat tttaatacag
     2461 cctgatatgg ttaaccaagt tgcggaacaa gctacatcgg gctatgagtt accaaacgaa
     2521 gaatccaatg tattgcaaat gccgaccatt cgcttaaata ccgctatcga aaacgaggtg
     2581 gatacaatcg aaaacagttc atcattggaa gacctgaaaa tagcagaatc tatgttacct
     2641 caaattcagc ttgataatga tattgaagat actgaagaag ctattgacga tgtgcgtgaa
     2701 attacaccgg aatttatggt aacgccgccc aaggcgatga aagatgtgtc tgagcagcca
     2761 gcctatccaa aaggctatgg tgatacgtta attcacccgt tgttacaacg aaaagtgata
     2821 accgaaaaac cgacaacacc attgccaacg ctagatttgt tggataaagc accgattcaa
     2881 agccagcaaa ttaccgaaca ggaaatacga gaaacctctg tgcggctaga agcagaatta
     2941 gcaaactttg gcgtaaaagc taccgttgaa gatgtattgg tcggtccggt agtgacccgt
     3001 tatgaaatcc aaccggctgc cggggtaaaa gcctccaaaa ttaccaattt agcgagcgat
     3061 atagctcgtg gcttaatgtt taaagcgatt aggattactg aagttatccc aaacaaacct
     3121 tatatgggta ttgaaactcc gaataaacac cgtgaaacag tgtggttgcg tgatgtgcta
     3181 gacagcgatg aattccgcaa taccactgca acattgccaa tggcactagg taaagacatc
     3241 agtggtgagc ctgtggtggt cgatatggcg aaaatgccac acttactggt ggcagggcag
     3301 accggtgggg gtaaatcggt tggggtgaat acgatgattt taagtctatt gttcaaactt
     3361 acaccagaac aagtccgctt tattatgatt gaccctaaag tggtggagct ttccatttat
     3421 aacgatattc ctcacttatt aacgccggta gtaaccgata tgaaaaaagc cgccaacgca
     3481 ctacgctggg cggtggagga aatggaacgc cgttatttgc tggtaagcca tttacaggtg
     3541 cgtaatattg aaggctataa tgcgaaaatc gaccaagcgg cagcgatgaa tttgccgatt
     3601 ccagatccaa cgtggcgacc gggcgattca atggatagcc ttccgcctcc gctacaaaaa
     3661 ttaagctata ttgtgctgat tgtcgatgag tttgcggatt taatgatgtc tgccggtaaa
     3721 gaagtggaag agtacattat gcgaattgct caaaaagccc gagcggtggg aattcactta
     3781 attcttgcta cccaacgtcc gtcaaccgat gtgattaccg gcgtgattaa agccaatatt
     3841 ccaagccgga ttgcgtttac agtagcgagc caaattgact caagaactat tttagattca
     3901 ggtggagcag aggcattatt agggcgaggc gatatgctct attcaggagc tggcagcccg
     3961 gatattattc ggatacacgg tgcgtttatg aaagacgaag aagtgcaacg cgtggcggat
     4021 aattggcggg caagaggaaa accaaactac ttggaaagca ttgtggaatc ccgctcggaa
     4081 gaggctgatg gcaaaaacga tggcggaacg ggcgatttag atccgctatt tgacgaggtt
     4141 gttgaatata tcacggaaac cggttcggtc tctatcagta atattcaacg ccgcttctcg
     4201 cttggtttta accgtgcggc tagaattgtg gatcaaatgg aagctcaagg cattgtttct
     4261 gagccactta aaggtggcaa acgagaagtg ctggcgaggt aaaacacata caagcggtta
     4321 gatttctgtg aaattttgca aattttgaca aaaatctaac cgattgtatt tatctaatgc
     4381 taaataagca tagtataagg ctagtctttg ggctaaaatt aacgcaattt ttacatcttt
     4441 taaggttgaa tatatgcgat tagatgatat taaagaaagt aaaaatgttg aagacagaag
     4501 attcaacaat gtgggtagaa ctaaggttgg tagagggaaa ggtggaatat taacctttat
     4561 tatcgtgctt gttggagctt attatggtgt tgacttaaca ggtttaatgg gcggtggcgt
     4621 agaatatgag gagcagacat cagcattaaa tgaaagtgaa gaaaaacagc taagaacctt
     4681 atccgctaaa gtattaacga ccacagaaaa tatttggact tcctacttcc agcaaaatgg
     4741 tctaacttat aaagagccgt ctttggtgct ttaccgtggg gcaattcaaa ctgcctgtgg
     4801 caccggtcaa tctgcaatgg gtcctttcta ttgccctgtc gatcaaaagg tttatttaga
     4861 tttatcattc tatgatgata tgcgtaaaaa attaagagca tcgggcgatt ttgccttctc
     4921 ttacgtgatt gcacacgagg ttggccacca cgtccaaaat ttattgggca taacaagcaa
     4981 aacccaacgg gctcaaatga aagcctcatc taaagccgaa gcaaaccaaa tttcagtcaa
     5041 tgtagaattg caagctgact gttttgccgg cgtttgggga caccaactcg caaaagccaa
     5101 ccgattagag gaaggcgatg tggaagaagc ctttaatgct gcccaagcag ttggagatga
     5161 ccgattacag caacaaagtc gtggctatgt cgtgccggat agcttcaccc acggctcatc
     5221 tgcacagcgt ttggcttggt ttagaaaagg tttaaccaca ggaaatcctg cagtttgtaa
     5281 tacatttaat taaaaaataa ttaaattatt tgaaatcttt ttattatcgt actactatga
     5341 tagtacgatt ttattttcat tttatttaga gagtgaacac aatgtcttat atcaaaaatg
     5401 acgatgtcag aattaccagt attgaagaat tactaccacc tattgcatta ctggaagcct
     5461 atccggcaag tgatgttgca gcggaaaccg ttgctaaaac ccgtcaatca atccacaaaa
     5521 ttttacacgg tgcggatgat cgcttattag tggtgattgg accttgctcc attcacgacc
     5581 cgaaagcggc attagaatat gcacaaaaaa ttaaagcaat gcgtgctgat ccgaaaatta
     5641 accaaaactt agaagtggtg atgcgtgtct attttgaaaa accccgtaca accgttggtt
     5701 ggaaaggctt aattaacgac ccttatttga acgaaacctt tgccttaaac gatggtttac
     5761 gcattgcccg taaagtatta tctgatatta atgatttaag cgtgccgact gccggcgagt
     5821 ttttggatat gattacacca caatatgtgg ctgactttat gagctgggga gcaattgggg
     5881 cgagaacgac tgaatctcaa gtccaccgtg aattagcctc tggtttatcc tgtgcggtag
     5941 gttttaaaaa cggcacgaat ggtagtgtga aaattgcatt agatgcaatt tcttccgcaa
     6001 aatcgccaca ccatttctta tcggtcacta aattcgggca ttctgcgatt gtttcaacag
     6061 caggaaatcc tgactgtcac attattttgc gtggtggcga tagcgcaacc aactacgatg
     6121 ctgactccgt tgcagcggct tgtgctgcga ttgagaaatc cggcggacgt ccacacgtga
     6181 tgatcgattt cagccacgct aacagccaga agcagttcaa acgccaaatg gacgtttgtg
     6241 ctgatgtgtg caaccaaatt gcaggtggat cagactttat ttcaggtgtg atgattgaaa
     6301 gccacttggt agaaggtcgc caagacttaa ttgaaggcaa aggtttagag gcgttagttt
     6361 acggacaaag tattacagac ggctgcattg gctgggaaga tagtgaaaaa gcgttattcg
     6421 cacttgctga agcggttgaa aagcgcagaa attttgcaaa ataatctttt cttgaaccga
     6481 gggcgaatat aattcgccct ttatttgttt aaccttaaag caaatttaat ttattcaaac
     6541 taaacatatg atgaaaccca gcattcttgc tttagatact gcaacagagg cttgctctgt
     6601 agcgttacag tataacggaa atatcacgtt cttagatgaa atcagccctc gttcccatac
     6661 tcaacgtatt ttgccgatgg tagatgaatt acttaatcaa gccaatatcg aactcaaaga
     6721 agtaaattat ttagtattcg gacgtgggcc gggcagtttt accggtgtga gggtcggtgt
     6781 aagtgtggca caagggttgg caatgggagc gaatttgccg gtggtagctg tttctaactt
     6841 aaaagcaatg gcagaagaag cataccaaaa attaggtgca acaaaggtaa ttgccttaat
     6901 tgatgcccga atgaatgaag tttactttgc tcaatttgaa cgaaagggtg aagaatggaa
     6961 tgaagtggtg gctgaacaag tctgttcgcc agaagtcgca ataagtcaat tccaaatgag
     7021 tgaagagact gtggtggttg gcacaggttg ggcggcatat tcgcaatttt ctgagcaaaa
     7081 tctaccgctt gttatcagtg atattacctt gccttccagc cgatatatgc tttcgattgc
     7141 tgaaactgag attcaaaaag gcaatatcca atctgctttt gaaatagaac cggtgtattt
     7201 gcgtaatgaa gttacttggg aaaaactacc tcataagcaa taatctattg aacttatggt
     7261 taagtgaata aatattgcaa aaagcccata taacaggcaa tagtgaatag gattacacca
     7321 agtaatttag tagttaattc gggactcaag atattgcgta atttcattga aaaatagaca
     7381 gtaaagagag aacttatcac tacggtgccg acaattgcaa aattcacata gccgatttgc
     7441 caagttgcta aggttgaatt caacacaaaa gggcgggata agtagccata taaattgccg
     7501 attccgccaa tcaccatcat atagttagta taaactgcaa cctgtttcgg tgaaaccgag
     7561 cgtatttgcg tgacaatcgg agccataatc gaaccgccgc caatgccggt aatacctgca
     7621 attaaaccgc ctaaactgca cattgtggca ttagatactg cttcttttgg tttaggtggt
     7681 actattgctt taactcttgg tttgtgaaat agggttttaa atgaaagtgt cagcagggtt
     7741 agaataaaga tagcgataat gctgttatca gagaaaagaa aactgagttc aaacccaatt
     7801 tgtacgccta acatcatcgc aagtgaccaa atcaacacat tttttcgatt gatttttatc
     7861 tgttgatggt ggaataagca taaattgata gtggctgaac caatcacaat ggtaagagag
     7921 caggcggaaa tcacttggag aggcagttca ggaaacagtg aatgtaaaat cggcatcatt
     7981 aaaacacccc cgccaatccc aaatagtgca gaagtgatat tgactataat gccacaaatg
     8041 atcaaaataa ttatcacggt cattgtcata atccatctcc taaagtgtga taag
//
LOCUS       DANLXQ010000112         8083 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.112, whole genome shotgun sequence.
ACCESSION   DANLXQ010000112 DANLXQ010000000
VERSION     DANLXQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.112"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            32..946
                     /locus_tag="PXD94_001900"
     CDS             32..946
                     /locus_tag="PXD94_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6286736.1"
                     /translation="MPSSYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGIRFLV
                     GGFTLLFFILWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTVSI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSVFLFLSIIIRNIQNLVVKFARELNVLTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMVAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLVKYPKHY"
     gene            1065..1925
                     /gene="araC"
                     /locus_tag="PXD94_001901"
     CDS             1065..1925
                     /gene="araC"
                     /locus_tag="PXD94_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDL6286737.1"
                     /translation="MYKNLSLLSPTYPFDLEFIAGLTQCEKGNYLDSIVDRQQGMHGY
                     MLQLTTFGRGSVFDGKHVFTAHRGQLLLFTPNAVQHYHRHPESQYWHYKWVYFQPNPK
                     WNKWLNWSDMEEKIGRITISDDRYFQEISQLFSKIELELKSSKFFKEDIATSLLEYLL
                     MKCISAEKIEVIPVIDQRILTVCDLILTNLAKNESLEAMAEKVFLSPSRLSHLFKQSL
                     GISLVQWRELQRISESKKLLYFSNISISNIAKSLGYEDSLYFSKIFKKHTGLSPSEFR
                     TLERRKDSEM"
     gene            2112..3086
                     /locus_tag="PXD94_001902"
     CDS             2112..3086
                     /locus_tag="PXD94_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL6286738.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLNVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            3141..4631
                     /locus_tag="PXD94_001903"
     CDS             3141..4631
                     /locus_tag="PXD94_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL6286739.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            4615..5574
                     /locus_tag="PXD94_001904"
     CDS             4615..5574
                     /locus_tag="PXD94_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6286740.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            5577..6527
                     /locus_tag="PXD94_001905"
     CDS             5577..6527
                     /locus_tag="PXD94_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6286741.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASAFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            6540..>8083
                     /locus_tag="PXD94_001906"
     CDS             6540..>8083
                     /locus_tag="PXD94_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL6286742.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTIRTAKALGISAMMHGYLPFDK
                     HGNPLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYSLEEYLDNCIFNTTKLNLVQPDKTMSEGY
                     ERFMQR"
BASE COUNT         2380 a         1544 c         1590 g         2569 t
ORIGIN      
        1 tttacagctg agccattttt gctttttgaa tatgccttcc tcttatcaat tagtcttaat
       61 taacctactt tatatcttcg gtattggtct aaatattgta ctgcttaggt ttctcagttt
      121 gcattttgac ccattaaata ataacggtat acgttttctt gttggtgggt tcaccttatt
      181 atttttcatt ttatggagat tccgcacatc gctgtctcag ctgattaaaa ctccaaaatt
      241 gattgcgatt tctctatttg tcgggttaat gatgtccgca aatatgtatt tttggctaaa
      301 aggggtcaaa attacaaatg ctgttaccgt ctctattttt ggggtattag ctatgccatt
      361 tggtgtgatt attgccgcca tattctttca agatgaaaga gaacgcatta aaagcaaagc
      421 tttctggttc ggctcaattt taacgatcat cggttcactc ggatttattt ggcacgggaa
      481 aacactctcg attggcgaag gatttattct tggttctgtg ttcttatttt tgtcgatcat
      541 tatccgtaat atacaaaacc tagttgtaaa atttgctcga gaactgaacg tattaaccct
      601 aagttgtgta acatctttaa ctacttcact ctcgagtcta ttacttagcc aacaagcagg
      661 taaatttaat gagcttttta ctgcaccaaa ctggctacta ttctctctga tggttgctgg
      721 tatttatgcg attatgatcg gaatggtatt aacctttcac attatccaaa cccaaggact
      781 gataacctac caagtattgg aactattaat gccggtttca acagccatca ttgcttatat
      841 tttactggac gaacgtattt ccattgttca aatgggcttt gcttgtattg tgattttagg
      901 ctcaagtatc gccttaaagt tagtaaaata tcctaagcac tattaggatc tttgctaatt
      961 aatattggta aaattacatt tggtttacta atctgagcgt tcattcatta gtaatgataa
     1021 acaagcggtt attttttgtt attttttggt aaatactgat agtcatgtat aaaaaccttt
     1081 ctttgctttc accgacttat ccatttgacc ttgaattcat cgccggtctt actcaatgtg
     1141 agaaaggtaa ctatcttgat tctattgtcg ataggcaaca aggaatgcac ggttatatgc
     1201 ttcaattaac cacttttggt cgtggttctg tttttgatgg taagcacgtt tttaccgctc
     1261 atcgagggca gttactcctc tttacaccca atgccgttca acactaccac cgtcacccgg
     1321 aaagccaata ttggcactac aaatgggtct attttcagcc gaatcctaaa tggaataaat
     1381 ggctgaattg gtcagatatg gaagaaaaaa taggacgtat caccattagc gatgatcgtt
     1441 actttcagga aattagccaa cttttcagta aaattgaatt agaacttaaa tccagtaaat
     1501 tttttaaaga agatattgca acaagtttgt tggaatactt acttatgaaa tgtatttccg
     1561 ccgagaaaat tgaggttatt cctgtcattg atcaacgcat tttgacagtt tgtgatttaa
     1621 tcttaacgaa tttggctaaa aatgagagct tagaagcaat ggcagaaaaa gtatttttat
     1681 cgccatctcg cctctcacat cttttcaagc aatcgctggg gatcagctta gtgcaatggc
     1741 gggaattaca acgcatttcc gaatccaaaa aattgcttta tttctctaac atctctatct
     1801 ctaatattgc gaaatcctta ggctatgagg attctctcta cttttccaaa attttcaaaa
     1861 agcataccgg gctgtcaccc tctgagttta gaaccttaga acggcgtaaa gacagtgaaa
     1921 tgtgatcttg atctcacttt actttgcata gcagaatttt ccatatcgat aacaaaaagt
     1981 tacctgtttt tctctcattc aaaagcataa gatacaaaca gctttaatag tgctgtttct
     2041 attagagaag ggatatcaag ctctgtgagt gatattcagt gaaataactt cttatgtatg
     2101 agggaagaac aatgaaaaaa ccaacgttca agcacgcatt attttcaagt attttagctt
     2161 tagcctgtta cggctcggca tttgccaatg atgttgttaa tatctccaaa attgacggta
     2221 tgccgtggtt taatcgtatg gcagagggtg ttgtgcaagc aggcaaagat aacaatatca
     2281 atgcttatca agttggtcca tcaaataccg atgcaccaca acaagtaaag ttaattgaag
     2341 atttaattgc gaaaaaagtt agtgctattt ccatcgtccc taacgatgca aatgccttag
     2401 aaccggtact gaaaaaagcg aaacagaacg gtattgtggt attaaccaat gagtcagtcg
     2461 gacaacctag tgccgattgg gatattgaaa ttatcgataa tgcacaattt gcagcagact
     2521 atgtagaaga agtagcaaaa gctatgggcg gaaaaggcgg ctatgtgatt tatgttggtg
     2581 gtttaaccgt gcctcaacat aatctgtggg ctgatttatt cgtgaaatac cagaaagagc
     2641 attatccgga tatgtttgaa gtgaccagca gaatgcctgt tgccgaaaat attaatgatg
     2701 cacgccgtac caccattgat ttagtgaaag cccattccga tcttaaagcg gtcgtttctt
     2761 ttggttcaca aggtcctatt ggtgcagggt tggcagtgaa agaaaaacgc ttaaaaaata
     2821 aattaaatgt cttcggaatg atgattcctt cccaagcagc caatttaatt aaaagcggtg
     2881 atattacaat gggaattacc tatgacccgg ccaatgcagg ttatgcttta gctgctgtcg
     2941 ctgccaaagt attaaaaggc gaaaaaattg aggatggatt agagatccca acggtcggta
     3001 aagccaaagt agatagtgag aaaaaactac ttcaattcca caatgtgctg aaagtcacca
     3061 aggaaaatat cgataaatta tattaatttg cttgaaatat gggctgatag ccagcccata
     3121 ttttccctca ggaggcactt atggcattta tttcgatgcg gcatatcagt aaaacattcc
     3181 acggcataaa agcattaaat caagtcaatc tctcattaga ttatggcgaa gcactttgtc
     3241 ttgccggtca aaatggctgt ggaaaatcaa ccttgatcaa aattctttcc ggcgtttatc
     3301 aaccggataa aggggctgaa attcaaattg gtgcaaccca atataccaag ctgacaccac
     3361 aggaatcgat tgataaaggt attcaagtga tttatcaaga tttagccctc ttccccaatt
     3421 taaccgtggc agaaaatatt gccattaacc tgcaccgaaa atttggctgg gtgagtcaag
     3481 atcacattca tcaggttgcc ctcactgcta ttcaaagcat taatgcaaat ctagacctga
     3541 atgcaatatt agaagattta ccgatcgctc aacaacagtt agtcgctatt tgtcgagcgt
     3601 tagcacaaaa tgccagatta ttgattatgg acgagccgac tgcctcactt actgcaaaag
     3661 aggtcaaaga tctgttagat gtcgtcatca aacttaaaag caaaggcatt agcatcatct
     3721 ttgtcagtca taaattgcaa gaagtaatga gtgtttccga tagcgtatta gtgctgaaag
     3781 atggcaatat ggttgggcaa tatcctatcc gtgaaatgaa tgaaaaacgc cttggtttcc
     3841 taatgactgg gctggaaatc gattacaagc ggttagattt gccggatttt ttgcaaagaa
     3901 aaaccgtatt ggaagtccgc aatttaacgc tgcctcatca gtatcaagag attaactttt
     3961 cgctacgaga aggggaaatt gttgccctta caggattgct cggttcaggg cgtactgagc
     4021 tttgcctaag tttattcggc attactcaac cacaatccgg cgatatttta ttaaacggtg
     4081 aaaaagtgat atttaacact aaccgtgatg ccattgccaa agggattgct tatgtatcgg
     4141 aagatagaat gcacaccggg ctgattatgg cagagtcgat tcatcacaat attatttcga
     4201 ctatttttca caaaatcacc gataaattac agattattca atcctcgaag gcttatgcaa
     4261 gtagccaagc cttgattgat tcattaaaga ttaaagtatc cgattcagat ttgcctgtta
     4321 ataccctttc cggagggaac gcacagcgag tttcgattgc aaaatggctg gcaatcgacc
     4381 ccaaaattat tattttagat gccccgacta ttggggtcga tatcgccaat aaagaaggga
     4441 tcttccaaat tattaaatca ttggctcagc aaggcattgc agttattttt gtgactgatg
     4501 aagtggaaga gacctactac aacagccatc gggtaattgt aatgaaaaaa ggtcgcattg
     4561 ttgatgaaat cttgccggca tattccagtg aaaaatcgat tgcggaggtt atttatgaaa
     4621 atcagcaata atcatattct ttccagtacg ttactgattt taatcctatt attaacccta
     4681 tttactcgtg attttgcaac tattgagaac ctttatgatg ttgtaaataa ttacgcaatg
     4741 ttaatgattt tagcttgtgg tttatttgtg gtattgctgt ctggcggcat tgatatttct
     4801 ttccctgcta tcactattat ttcccaatat gttatggtca ctctgatcgg taaatatgat
     4861 gtaggatttg ccggcgtttt tgttatttct tccaccatcg gcatattatt aggttttatt
     4921 aatgctctat ttgttaatcg gcttagtgtc ccttccatta ttattacgat ctcaacactc
     4981 aatatttttt atggcggact actctatttt accaaaggtg tatggcttta tgagtatccc
     5041 gaatggttta cccaagaaat cgtgttcttt aaacagataa ctgctgaggg agtggaattt
     5101 ggattatcgt ttcaagcatt aatggctatt ttggcggtga tattaaccgg cttaattacc
     5161 aataaactca ctattggtcg aaaaatttac gctcttggtg gtaatccgga tgcaacctcc
     5221 cgtattgggt ttaatctctt atttctccat ctatttgcat acggttatat gggctttatg
     5281 gcaggcttgg caggcatagt gcaatcttat accgtacaat ctgtcgcacc ggattcacta
     5341 ctgggttatg aattaaccgt cctagctgcc gttgtattag gtggtgctag tttatcaggt
     5401 ggtagaggca ccctgcttgg tacagtaatg ggcgtaatgt tactggcgat tattcaaaac
     5461 gggctaaatt taatgaatgt atcgtcttac tggcaaacca ttattaccgg tatcatcatt
     5521 atcttaagta ttagtgcaac agccttaagt cagcggaaaa aacaaggagc ataacgatga
     5581 aaagtaaact ggatccaacg attttctatc tcttagcact cttcgcagtt gcagttatcg
     5641 cctttagtat tttcattccg gatatttttt ggtctgtcag taactttcaa tctatggcat
     5701 cacaaattcc agtattaggt attttaaccc tagcaatggc attaccgatg ttaacaggtg
     5761 gaataaatct ttccattatt gccggaatga atgcttgctc attaattatt gcctacttta
     5821 ttacgcaata taccggtatt tcttggttat ttattgctat tttattaagc ctttgctgtg
     5881 ccttagccat tggattactc aacggcacat taattgccat tattcgtgtt tcaccaattt
     5941 tggcaacctt aggcacaatg actttaatta acggcattaa tattctagtc actaacggtt
     6001 caaccattgc gaactttccg gaaacaatgt tgagtattaa tgcttcagcc tttttatgga
     6061 ttccgctacc cattctaatc tttctctttc ttgctgggct gatttggttt ttcttagaaa
     6121 aaactgcaat gggtaaagcg gtttatttca tcggcaataa tgaaaaagcc acctattatt
     6181 cgggtattaa taccaaaaaa gtgctgattt gggtttatgt tatctcctca attctttgtg
     6241 tatttgcagc tattttgatg atgtctaaac tcaattctgc aaaagcctct tatggtgatt
     6301 cctatttgtt gatctccatt ttagccgcag tattgggcgg tattaaccct gatggaggta
     6361 aagggaaact attagggatt atgctcgcct tagttttatt acaagtgatt gaaagtggct
     6421 taaatatgct cggcattagc agttacatta caatgatttt atggggaagc ttattgttat
     6481 tctttatctt cctccaaaaa aatcgccttt tccgactaac acagtaatgg tggtagatta
     6541 tgcaaaatga aaaacaactc attgaacaag gtaatactgt tattggtatt gaattgggct
     6601 caacccgaat taaagcggtg ttaattagct cagatgggac gattttagca accggtgggg
     6661 cggattggga aaatcgactg attgacggta tttggaccta tcatcaaaat gaaatatggg
     6721 aaaaactaca agctgcttat atggatttaa gcaagacagt caaagaaaaa tatcacacaa
     6781 ccattcgcac agccaaagca ttgggaatta gtgcgatgat gcacggctac ttacctttcg
     6841 ataaacacgg aaatccgctg gttgccttta gaacttggcg aaacaacatt accctaacct
     6901 catcggaaaa attgaccgca ctctttcaat acccaattcc gcaacgttgg agtattgctc
     6961 atttacacca agcattgctt aaccaagagc ctcacttgcc ggaaattgat tacctgacaa
     7021 ccctctcagg ttatgtgcat tggcaactga ccgatcacaa agtgttaggg attggcgatg
     7081 cttccggcat gttcccgata gatactgagt tgaaatccta caatccaaca atgctggctc
     7141 aatttgatcg agccattgcc gactaccgta tgccttggaa attggaagcg attttgccac
     7201 aagttcgtgt tgctggtgaa caagcgggca aattaaccga aaaaggtgca aaattgttag
     7261 atccaaccga taatttgcaa gctggcattc cactttgtcc acctgagggt gatgccggaa
     7321 caggtatgat tgcaaccaac tgtatcaaag aaaaaaccgg taatatttct gccggcacct
     7381 ccgcatttgc aatgattgtg ttagaaaaag ccctatccaa agcctattca gagctggata
     7441 ttgtgacgac cccggcaggt aaattagtgg caatggctca cgctaataat tgcacctctg
     7501 atattaatgc gtgggtgaat ttattcggcg aatgcttatc ggtttttggc gtaaatatct
     7561 gcaccgaaga actttatgaa atgttatttc tccatgcctt gcaaggcgat gcagattgtg
     7621 gagggctatt atcctacggc ttccactccg gtgaacataa tgtcggttta tccgaaggct
     7681 gtccggtttt cttgcatcca acgaatgccc attttaactt agcaaacttt atgcgaaccc
     7741 atctctatac cgcttttggt gcaatgaaat taggaatgga cattctcatt aaccaagaaa
     7801 aagtggaaat ttcccgtatt ctcggtcacg gtggtatatt taaaacagaa ggtgttgcac
     7861 aaaaaattct gtcttccgca ctgaatattc cattggctac cgcaagtact gcttcaaatg
     7921 gcggagcttg gggaattgca ttactggcag actttctcag cgtatcagac cgttacagtt
     7981 tagaagaata tttagataat tgcattttca acaccaccaa actcaatctg gttcaaccgg
     8041 ataaaacaat gagtgaaggt tatgaacgct ttatgcaacg tta
//
LOCUS       DANLXQ010000113         8062 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.113, whole genome shotgun sequence.
ACCESSION   DANLXQ010000113 DANLXQ010000000
VERSION     DANLXQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.113"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..520
                     /gene="proQ"
                     /locus_tag="PXD94_001907"
     CDS             <1..520
                     /gene="proQ"
                     /locus_tag="PXD94_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL6286743.1"
                     /translation="QKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPLKVGLFQ
                     ELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVTEDEATH
                     AAEALAAAKAVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNLPKASEE
                     KLAALADKFGRK"
     gene            606..2618
                     /gene="prc"
                     /locus_tag="PXD94_001908"
     CDS             606..2618
                     /gene="prc"
                     /locus_tag="PXD94_001908"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL6286744.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKIFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTLWEQRVKNDIINLYLKDKKWPEIKKTLTKRYNFAIKRLTQVKADDILQTYLN
                     SFSREIDPHTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSK
                     KLVVGDKIVGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIIT
                     LVRDKVRIEDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLII
                     DLRENGGGSLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINR
                     HSASASEIFAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQK
                     FYRINGGSTQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAV
                     AALTAKHQERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDI
                     NARFAREGKKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
     gene            2736..4241
                     /locus_tag="PXD94_001909"
     CDS             2736..4241
                     /locus_tag="PXD94_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL6286745.1"
                     /translation="MVAFSGMALAETATVAQVASEPVVQDNILPQLSFAAQQARAAEL
                     VKQADYALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLDSEFATMWND
                     KAAEKAFLKEYALFAVSGVSSKSAKALQQILNQPEGLGRDILLTDGFLDYLYYNKNVF
                     KNANNWLYNLGSYVPKSPSSEQIFQWTNAVREGDSGQFVTDLVPRNHLYRDTAQRVLA
                     MAGGSSAPKAKKGKAVTEVAGGGNGASFYKLALNAQRLRIIPSFNNGIFVNIPSYQLY
                     YFRDGNLALQSKVIVGREDRRTPVMYSKLSNVVVNPPWNVPTSIMTKDIIPKLARDPG
                     YADRASFEILDSSGNKINPRSVNWSQYLNAKSSPYRIRQKAGDDSALGRYKFNMPSSD
                     AIYLHDTPNRGLFGKTGRALSSGCVRVEKSDELATILLKEAGWGSDKKNQVLGSKKTT
                     SAQIRSDNPVYLYYVTAWVEGGKVQTLPDIYNFDAKIPQHSVNWGKLKSII"
     gene            4411..4965
                     /locus_tag="PXD94_001910"
     CDS             4411..4965
                     /locus_tag="PXD94_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL6286746.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
     gene            4982..5611
                     /locus_tag="PXD94_001911"
     CDS             4982..5611
                     /locus_tag="PXD94_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL6286747.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(5646..6017)
                     /locus_tag="PXD94_001912"
     CDS             complement(5646..6017)
                     /locus_tag="PXD94_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL6286748.1"
                     /translation="MRISYFAFAIFSIFLTACGSKPVSYLPTGSEPIVNIESDIAEKI
                     ELETNSKQFSTTNLTEFPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQT
                     IALIKPTEESHNYRLYLRGVK"
     gene            complement(6020..6379)
                     /locus_tag="PXD94_001913"
     CDS             complement(6020..6379)
                     /locus_tag="PXD94_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL6286749.1"
                     /translation="MTTEYQKTIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLAQEEGIAEEGYRLVMNCNKNGGQEVFH
                     IHMHLLGGQKLGPLVAK"
     gene            complement(6392..6991)
                     /gene="rnhB"
                     /locus_tag="PXD94_001914"
     CDS             complement(6392..6991)
                     /gene="rnhB"
                     /locus_tag="PXD94_001914"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL6286750.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            complement(6988..8025)
                     /gene="rsgA"
                     /locus_tag="PXD94_001915"
     CDS             complement(6988..8025)
                     /gene="rsgA"
                     /locus_tag="PXD94_001915"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL6286751.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQEVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
BASE COUNT         2464 a         1446 c         1757 g         2395 t
ORIGIN      
        1 gcaaaaagta gaacaaactc agaataaaac caatccaagt accaaggaag tgattgctta
       61 tttagcggag aaatttccgc tttgtttcac gcttgaaggt gaagtaaaac cattgaaagt
      121 tgggttgttt caggaattag ctgaagcatt aaaagatgat gagaaaatca gtaaaacgtt
      181 attgcgccaa gtattgcgga attacactaa ttcttggcgt tacttggctg ccagccaagc
      241 taatgttgct cgtgtcggct tacagggtga agaagttggc gtggtaacag aagacgaagc
      301 tactcacgcc gcagaagctt tggctgctgc gaaagcggtg gttgcagaac gtaaagcaca
      361 agagcgtaaa gcacaacgta aagagtactt caaacagaaa gcacgtgaag aaaatgcgaa
      421 aaagcgtgca gaatttaagg cgaaaaaagc aatagataat ttacctaaag cttcagaaga
      481 aaaattagct gcattagcag ataaattcgg tcgtaaatag ttttcaagtc gctttgtgct
      541 tacaagcggt cggattttag taaaattttg caaaaaaatg accgcttgat tatttggaaa
      601 agaagatgaa attaacaaga ttaaataaaa tttttgccct agccctaggt ttaatgatag
      661 gaaacgcttt tgccgttcag ccggacatta aagaaagtgc gattgtgatg ccaaaaccta
      721 atgagcagca tagcttatca accaagcgtg taacagcccg attaactcaa tcgcattatc
      781 ataaatttca actgaatgat gagtttgcag ggaagatttt taaccgctat gtcgattggc
      841 ttgatccttc tcataatact tttcttcaag ccgatattga tgaaatgcgt agtaaatatg
      901 ctacaacatt agatgatgat ttatatgctg gtaaattaga tgcagcattt gcaatgtatg
      961 agttaatgtc taaacgtcgt tatgagcgtt atagctatgc cttatcttta ctagataaag
     1021 agcctgactt aaaagggaat gatgaaattg agattgaccg tgagaaggcc gcatttccaa
     1081 aaagtgtaga agaggcaaat acactttggg agcagcgagt taaaaacgat attatcaatt
     1141 tatatcttaa agataaaaaa tggcctgaaa ttaagaaaac actcactaaa cgttataact
     1201 ttgcgattaa gcgtttaact caagttaagg ctgatgatat tttacaaact tacttaaatt
     1261 cattctctcg tgagatcgat ccccacacta gctatttgtc tcctcgtgcg gcaaaagcgt
     1321 tccaagagag catgaatctg tcgctagaag gaattggtgc aacgctaacc caagaagatg
     1381 atgtaaccag cattagagca ttagtgccag gagcccctgc cgctaaaagt aagaagctgg
     1441 tagtcggcga taaaattgtc ggtgtaggtc aaggtgagag aggtgagatt gaagatgtta
     1501 tcggttggcg tttggataat gtagtcgata aaatcaaagg taaaaaaggc actaaagttc
     1561 gtttagaaat tgagcctgaa aaaggtggaa aaactaaaat tattaccctt gtgcgtgata
     1621 aagttcgtat tgaggatagt gctgctaaat taacggtaga aaaagtagat ggaaaaaatg
     1681 taggggtaat taagatccct actttttata tcggtttgac ttcagatgta cgtaaattac
     1741 ttactcaaat agagaaaaaa aatgttgaag gattgatcat tgatttacgt gaaaacggtg
     1801 gtggttcgtt aactgaagtg attgaattaa ctggtttatt tattaaagat gggcctgtag
     1861 tgcaagtaca agacgcattc aatcgtatca aagtacacga agatccagaa tcaaaatcag
     1921 tttatgatgg aaaagtgatg gtgatgatta atcgccatag tgcttcagcc tctgaaatct
     1981 tcgctgcggc aatgcaagat tacaaccgtg caattattgt agggcaacaa acctttggta
     2041 agggaactgt tcaacagagc cgttcattga attttgtata tgatttggat caagagcctc
     2101 ttggttttat ccaatatacg attcaaaaat tctaccgtat caatggtgga agtacccagc
     2161 taaaaggggt tgaggcggat attaaattcc cggaaatcat taatgctgca aaaacaggtg
     2221 aaagttttga agataatgcg cttccttggg ataaaattcc agcagctaat tataaagagg
     2281 ctgggaatgc tcgtaacgcg gttgctgcat taacagcgaa acatcaagag cgtattgcta
     2341 aaaacccaga gtttatagta ttaaacgaga atattgcgat tcgaaaagaa cgtgatgagc
     2401 gtaagttttt atcgttaaat ttagaagaac gcctgaaaga aaataaagca gatgaaagca
     2461 agcgcttaaa agatattaat gctcgttttg cccgtgaagg caaaaaagca ttaaaagata
     2521 ttgatgattt accaaaagac tatgaagcac cggacttttt cttaaaagaa gctgaacaga
     2581 tgatgcttga ttggtcagaa tttgataaaa aatcataaat gtgtttataa tgcgcacata
     2641 acgtaccgct tagctccgct aagcggttta tttttttata aatttgcaaa tattaagagg
     2701 aagagtgggt gaaaaccttt aaattattac ctattatggt ggcgttttct ggcatggctt
     2761 tggctgaaac agccacagtt gctcaggtgg cttctgagcc tgttgtacaa gataatattt
     2821 taccacaatt aagttttgca gctcagcagg ctcgtgctgc agaattggta aaacaggccg
     2881 attatgcgtt agaacaggaa caaattcgtc aagagcaagc aagaaaattt gcagaagcgg
     2941 aagcaagaat taagcaagaa ataggtaata atgccttatt gctgagccaa gcaacaacta
     3001 aggtttattt agacagtgaa tttgcgacaa tgtggaatga taaagccgca gaaaaagcat
     3061 ttttgaaaga atatgcatta tttgcagtaa gtggggtatc ctctaaatca gctaaggcat
     3121 tgcagcaaat cttaaatcaa ccggaaggtt taggtagaga tattttatta actgatggct
     3181 tcttagatta tttgtattac aacaaaaatg tgtttaaaaa tgcaaataat tggttatata
     3241 acttaggcag ttatgtacca aaatcgccct cttcagagca aatcttccaa tggactaatg
     3301 ccgtgaggga aggcgattct gggcagtttg taacagacct tgttccaaga aaccatttat
     3361 atcgtgatac ggcacagaga gtattggcaa tggcaggagg ctcttcagca cctaaagcca
     3421 aaaaaggtaa agctgttacg gaggtagctg gtggcggtaa tggggcttct ttctataaat
     3481 tagcgctcaa tgctcaacgt ttacgtatta ttccaagttt taacaatgga atttttgtga
     3541 atatcccaag ctatcaatta tattatttcc gtgatggtaa tttagctctt caatctaaag
     3601 tgattgttgg gcgagaagat cgccgtacac cggtaatgta tagtaagctg agcaatgtgg
     3661 tagtgaatcc accttggaat gtaccgacta gtattatgac taaagatatt attcctaaac
     3721 tagcgcgtga cccaggttat gcagatcgtg ctagtttcga aattcttgat agtagtggca
     3781 ataaaattaa tccgcgtagc gtaaattggt cacaatattt aaatgctaaa agttcaccat
     3841 accgtattcg ccagaaagca ggtgatgata gcgctttagg acgttataaa tttaatatgc
     3901 caagttcgga tgcaatttat ttgcatgata cgccaaatag aggattattt ggtaaaacgg
     3961 ggcgtgcgtt aagttctggt tgtgtccgtg tagaaaaatc tgatgaattg gcaaccattt
     4021 tattaaaaga agcgggttgg ggatctgata aaaagaatca agtcttaggc agtaagaaga
     4081 cgacctcggc tcagattcgt tcagataacc cggtttatct ctattatgtg acggcttggg
     4141 ttgaaggtgg aaaagtgcaa acccttccgg acatttataa ctttgatgcc aaaatcccac
     4201 aacattctgt gaattgggga aaattaaaga gcattattta atcgtgctat ttctgataaa
     4261 ttccaagaag atatcatctt cttggaactt tttgaaaata tagcttacat aaatttacga
     4321 ttgtaaattt ttactttaag tatggtgtat agaaattcgc tcatttgtga gtgattttct
     4381 ataaatcatt agctaaccta tgaggaaaaa atgaaacaaa ttaatgttca acgccgtaag
     4441 tggctatcac tcggcggact tgttttaggg gctagccttc tgccgggtca agtaatggca
     4501 gcgctttcta ctcctgcccc agctgcgtta cgttttcgta atgtgaatac aggcgatact
     4561 tacactgcta aatttggtgc aggtggatta aataaaaccg atttaagcca gttaaactat
     4621 ttaatgcggg atcgtcatat caatcaagtt aaagcaattg atccaaaatt atttgtcaaa
     4681 ttgaatcaat tacaacgccg tttaggtttc cataatgcgg aaatcctagt attaagtggc
     4741 tatcgctccg cacaaacaaa tgcaagactt cgtcgtagaa gccgtggtgt tgccagccat
     4801 agttaccata ttcttgggca agcagtagat ttccaagtat caggtgtgcc gttatataaa
     4861 gtaaaacaag cggctgagag cttgaataat ggtggcgtag gttattatcc tcgtagtaac
     4921 tttgttcacg tagatactgg cccagttcgc acttggagag gctaacaagc ggtcttattt
     4981 tatgtttaat ttgcaaatta ttcctgttag tccatttcaa caaaattgca cggtgatttg
     5041 ggacgaaaat aagaatgcgg ctattattga tggcggcgat aatgccgaag gcattattca
     5101 atttgttgaa tctcagcagc ttaaggttca gaaattattg attacgcacg gtcatttaga
     5161 tcatattatg gcggtggaaa agttgagaga gcattttcaa gtagaggttt tcggctcgca
     5221 tcttgcagat aaacctttat ttgaacgttt gccggaaatg tgccaacaat ttggattacc
     5281 accagtaaat ggctttttgc cggatcattg gttggaggaa ggtgatcggg tcgaagtcgg
     5341 cgagctgagc tataccgttc gacatttgcc gggtcatgct ccgggtcata tcggtttttt
     5401 tgattttgac aatcgtattg cttttagtgg cgatgtttta ttccaaaata gcataggtcg
     5461 aacagatctc tatttaggca accatagcca gttattggaa acgatcaggg aaaaaatgtt
     5521 tgatttagat gatgatttca tcatcatacc gggacatggt ccacacacaa ctatcgggcg
     5581 ggaaaaacag accaacccgt ttttgaaata aataaagaag cagagcagtt ggctctgctt
     5641 ttttgctatt ttacgcctcg taaatagagg cggtaattat ggctttcttc cgtcggttta
     5701 atgagtgcaa tggtctgttt ttgcttaggt tgcaaccata gatgttgcca gccgatacta
     5761 tctgatatac cgttatggga ttgtgttacc ccatctttat tataccaaaa taatttataa
     5821 gtaagattaa gcggaaattc agttaaattt gtggtgctaa attgcttgct attcgtttcc
     5881 agctctatct tttcagcaat gtcagactca atattgacaa taggctctga gccggttggt
     5941 aaatagctga caggtttgct accacaagcg gtcaaaaaaa tcgaaaaaat tgcaaatgca
     6001 aagtaggaaa tacgcatagt tattttgcca ctaaaggtcc tagtttttgt ccgcctaaca
     6061 agtgcatatg aatatggaac acttcctgcc cgccgttttt attacaattc atcactaagc
     6121 gatagccttc ttcggcaatg ccttcttctt gggctaattt tgccgccacc gtaaataaac
     6181 gccctagggc taattcatct tcattactca cgtgattgac ggttggaata aatttattcg
     6241 gaataatcaa aatatgcgtg ggtgcttgtg gagcaatatc acgaaatgcg gtcacaagtt
     6301 catcttgata gacaatattt gccggaattt ctttattgat gattttggtg aaaatcgttt
     6361 tttggtactc tgtagtcatt gtgttttcct tctaatcgat taaacctaat acttttctta
     6421 caggggcaaa actcttacga tgataaggtg ttgccccaaa ttctgctaat ttttcaaaat
     6481 ggagtttagt cgggtagcct ttgtgctgtg cgaagccgta ttgcggaaat tgttggtcta
     6541 attcgtccat ttcttgatct cgggcgactt tggcaataat cgaagcagca ctgatttcag
     6601 ccactaagct gtctcctttc accaccgctt gtgccggcat tggcagtttt ggcggacgat
     6661 tgccgtcaat tagcacaaaa tccggctgaa tatgcaagcc ggcaaccgca cgttgcattg
     6721 ccaacattgt cgcatttaaa atattcagct catcaatttc tgtcggctcg gcacgtccta
     6781 gcgaccagct cagagccttg gctttaatct cttctgccaa agcttggcgt tttttctcgg
     6841 ataatttttt ggaatcggtt aagccctgaa tcgggttatt cggatctaaa atcacggctg
     6901 cggtcacaac cgctccgact aaaggtcctc gtccgacttc gtcaacaccg gcgattaatc
     6961 gggcgttagg gtaaataaat tcattcatta tacttcttct tttctgaaat ggcgttggct
     7021 tttggtttca gacaggcttt caattaagcg atggtaattt tcaaaccgaa ttgcagaaat
     7081 ttcgccattt tcgacctctt gtcttaaggc acaacccggg tcggttttgt gtttgcaatc
     7141 acggaatttg cacgtgccta aaaccgattg aaattctcga tagcctaagg tgatttgctc
     7201 aggttctaaa tgccataagc caaattctcg aatgcccggc gagtcaatca gctgtccgcc
     7261 ttgcggtaaa tggtataagc gagaggcagt ggtggtgtgc tgacctaacc ctgagttatc
     7321 gctcactgag ccagtgagag cattgacttc aggtaacagt tggttaatta ggctggattt
     7381 ccccacgccg gattgtccga caaaaataga cgtaccttta gcaagatatt ggtgtaacgc
     7441 ctgcatattt tcacccgtat cagccgataa acatagggtt tcatagccaa tctcttcata
     7501 gattttgagc tgctctttaa ctgcttgccg ttcgctttcg tttaataaat caattttgtt
     7561 tagcacgatt aaggcaggaa tattcgccgt ttcgcaaatc actaaataac ggtcgataat
     7621 attcagcgaa agtgtaggta gcacagctga tacaataatg atttgatcga tgttcgatgc
     7681 catcacctta ataccgtcat aataatcggg gcgggtaagt tcatttttac gtgggaagac
     7741 ggcttcaatg accccactaa tcccttgtag ttgctcacta cctttacgcc aagaaacctg
     7801 atcgcccacc actacatttt tcaatgtacg gcggatatta cagcggaaaa tttgcccctc
     7861 tgcggtttca acatcagcgt gtttaacgtg gcgagtaacg acgatgccct gttgcatttc
     7921 tcctaacatt tcatcttgcc attcaatctc ttttttgttg atacgcttat gatggttaga
     7981 ttggattcta cgctgttggt tatgggttaa tttcggttta ctcaaaagaa ctctcggtaa
     8041 ttattgcaaa tgaaaaatag gc
//
LOCUS       DANLXQ010000114         8017 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.114, whole genome shotgun sequence.
ACCESSION   DANLXQ010000114 DANLXQ010000000
VERSION     DANLXQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.114"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(52..1092)
                     /locus_tag="PXD94_001916"
     CDS             complement(52..1092)
                     /locus_tag="PXD94_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6286752.1"
                     /translation="MQTTLSPQQIAKKHRTIEKKRSFLLLFFLLICLLSLIFDIMTGP
                     SLLPFNEVWKALFQLGEIEETTKVIVYELRLPMAIMALVVGAALGVGGAEIQTLLNNP
                     MASPYTLGLAAAAGFGASIVIAFGGFGLPTEISVPVGAFVMTLFSSAILFAFSSLGRF
                     SSAILVLVGIALLFLFQSLLSLVQFISAPEVSQQILFWLFGNLTKATWQTISIAFGVT
                     TVCILLLFKDAWKLTALRLGEARALSLGINLTWLRMKTLLIVSVMTATCISFVGIIGF
                     IGLVAPHIARMLLGEDQRFFLPGSMLVGAAFLSMSSVLSKSLVPGALFPVGIITALVG
                     VQFFFWIVLAKR"
     gene            complement(1181..1771)
                     /locus_tag="PXD94_001917"
     CDS             complement(1181..1771)
                     /locus_tag="PXD94_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL6286753.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATRELS"
     gene            complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXD94_001918"
     CDS             complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXD94_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL6286754.1"
                     /translation="MVVDGNGSGKTTFSLALQGKISLYSGDYGNSFQHIQLLSFEQQQ
                     KMLEQIFKDRNNDSVSPDDFGLTARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHL
                     STGESRKVLFCQMLVSEPDLFILDEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRF
                     NDIPDAANHIELLDNLSLILQGERAEIEQQAVYSQLKFAEENVNVPLPDSAAPLLSLP
                     ENINPFELENVNIQYGEKKILDNLSWTVQPKQHWWIKGPNGAGKSTLLSIITGDHPQS
                     YANKVRLFGKQRGSGETIWDIKKNIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQ
                     QVPNALQLKAMEWLERLNLSNVAKKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQ
                     GLDGINRKLVKQFIEQLVTNSQTQLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEY
                     HQTALGA"
     gene            complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXD94_001919"
     CDS             complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXD94_001919"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL6286755.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPAGKKPMSVQDFLNGRADWFVSGTQLS"
     gene            4331..4942
                     /locus_tag="PXD94_001920"
     CDS             4331..4942
                     /locus_tag="PXD94_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL6286756.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNPSEVEAFADELTQRFSGKLALIKEEI"
     gene            4946..6430
                     /locus_tag="PXD94_001921"
     CDS             4946..6430
                     /locus_tag="PXD94_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL6286757.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNELRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLMVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            6431..6958
                     /gene="hemG"
                     /locus_tag="PXD94_001922"
     CDS             6431..6958
                     /gene="hemG"
                     /locus_tag="PXD94_001922"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL6286758.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGKLLANQL"
     gene            7090..7485
                     /gene="mscL"
                     /locus_tag="PXD94_001923"
     CDS             7090..7485
                     /gene="mscL"
                     /locus_tag="PXD94_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL6286759.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXD94_001924"
     CDS             complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXD94_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL6286760.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVAAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
BASE COUNT         2217 a         1600 c         1798 g         2402 t
ORIGIN      
        1 taagtttcca cgcttaatag ttacatcgtt taagttgagc atagaaaatc cttatcgttt
       61 cgccaataca atccagaaaa agaactgcac accgactaat gccgtaataa taccgaccgg
      121 aaataatgcg cccggcacaa ggctttttga tagcacggat gacatcgata aaaatgccgc
      181 acccactaac attgaaccgg gcaggaaaaa gcgttggtct tcgcctagca acattcgggc
      241 aatatgcggg gcaactaagc caataaagcc gataatgcct acaaagctaa tgcaggtggc
      301 cgtcattaca gacacaatca gcaatgtttt cattcgtaac caagttaagt taatgcctaa
      361 gctcaatgct cgagcttcgc ccaaacgcaa ggcagttaat ttccacgcat ctttgaataa
      421 taataaaata cagactgtag ttacgccaaa ggcaattgag atagtctgcc aagtagcttt
      481 agttaaatta ccgaatagcc aaaacagaat ttgttgggat acctcaggtg cagaaataaa
      541 ttgcaccaat gacagtaaag actgaaataa aaacaataag gcaattccta ccagcactaa
      601 tattgccgag ctaaaacgtc ctaaagagga aaaggcaaat aaaatggcgg aagaaaatag
      661 tgtcatcaca aaagccccga caggtacaga aatttcggtg ggtaaaccaa atccaccaaa
      721 ggcaatcaca atcgaagcac caaagccggc tgcagctgct aaacccaatg tataagggct
      781 tgccattgga ttattaagca aagtttgaat ttcagctccg ccaacaccaa gtgctgctcc
      841 aaccaccaat gccattattg ccattggtaa gcgtagctca tagacaatca ctttggtggt
      901 ttcttcaatt tcgcctaatt ggaataaggc tttccaaact tcattaaacg gaagcaggga
      961 agggcctgtc ataatatcga aaatgaggct gagtaaacaa atgagtaaga aaaagagtaa
     1021 taaaaaactg cgtttctttt caatagttcg gtgcttttta gctatttgtt ggggagagag
     1081 cgttgtttgc ataaaaatgt aatgtttgag aattattatc agacaggata agagaaaaga
     1141 tcgataaatt caagaatatc caagataccg actaaagtat tcaactaagt tctcgagtag
     1201 ccctgtcttt tacagtaaaa aactcctgaa tagcggcttc ttctaaaaga gtataattct
     1261 gccaaatttc ggttacagca tttcttaaaa tctcaccttt cttaactctt gatgacattc
     1321 ccaagaatac ctgttctaag aaatctaaat cggcataatg agttaatgct tttcgtgact
     1381 gtaaccagtt aaacatcggc acaaattgtt caggaaaaat cgtgcgttgt tgttcgattt
     1441 gctgaaagat ctgtgcttgt acctcattta aatctttagc aaataaccgt tggaaatgtt
     1501 tagcgagaaa atggtcgatc ataatatccg acacaatgcc tttataacgc ccgaatgtgc
     1561 cttggagtaa ttgggttgtg aggctgtccg gtcggtccga aatcttatca atcgagcggt
     1621 gtaacaccac accgcttttt aagttatccg ctaaattgag atgcggaatt aaacctttat
     1681 agaaatcacc ggcaaagttg ccatataggg tctgttgctt ggtttgggtg tcaatttcaa
     1741 gtgaaatcag cgaatgggta agaaaattca tttatgctcc aagtgcagtt tggtgatact
     1801 caaacccaaa ctcgttggtt tcgcctttcg gtacaaactc aaataaatgc gtaatgcaat
     1861 ttggagcatc gctgtcttgg tgcgaaacaa aaagtagttg cgtttggctg ttagtcacta
     1921 attgttcaat gaactgtttg actaatttgc ggttaatgcc gtctaaacct tgtaacggtt
     1981 cgtctagaat cagaattgat gggtgtttca ccattgctcg ggtaatcaaa agtaagcgtt
     2041 gttgccccca cgagagagag cgaaacggct ttttggcaac attgcttaaa ttgaggcgtt
     2101 ccaaccattc cattgctttt aattgcaagg cattcggcac ttgttgatac acgccgatgc
     2161 tgtcaaaaaa gccagaaaga atgacctcca ttgcggagca atttacccga taatccatat
     2221 gcaactggct gctgacatag ccgatatttt tcttaatatc ccaaatggtt tcgccacttc
     2281 cccgttgttt gccaaatagt cggactttat tggcataaga ttgtgggtga tcgccggtga
     2341 taatcgaaag caaggtcgat ttcccagctc cattcgggcc tttaatccac cagtgttgtt
     2401 taggctgaac cgtccaactt aggttatcta aaatcttctt ctccccatat tgaatattca
     2461 cattttccaa ctcaaacgga ttgatgtttt ccggcaggct gagtagcgga gctgcactat
     2521 caggcagtgg cacattgaca ttctcttctg caaattttag ctgagaatag accgcttgtt
     2581 gttcaatttc tgctcgctct ccttgcagaa ttaatgataa attatctaat aactcaatat
     2641 gattggcagc atcaggaata tcgttaaaac gatttacaat cagcactaac gccattttgc
     2701 tttgtagttc ggcaatcagt tgttgccaat attgcaccga ctgctgatcc agcccttcaa
     2761 acggttcgtc taaaatgaat aaatccggct cgctcactaa catttggcaa aatagcactt
     2821 tgcggctttc accggtggaa aggtgcataa acgggcgttc aagcagatgt tgaatatgca
     2881 atttgtgggc atattcctca cataacgcct cttttggact gccatttaaa attacttgtc
     2941 gtgcggttaa gccgaaatca tcgggcgaga cactatcgtt gttccgatct ttgaaaatct
     3001 gctccaacat tttttgttgt tgctcgaaag agaggagctg aatatgttgg aacgaattac
     3061 catagtcgcc ggaatagagc gagattttac cctgtaaagc gagtgaaaaa gttgttttgc
     3121 ctgaaccgtt tccgtctacg accacctaaa agtcattggg ttggatctgt aaggcgtcaa
     3181 tcgtgagttt atgttgttta accagtgaaa atcgggcatt ggttattgta agtgaaggct
     3241 gtgtttgcat atatttcccg ttttaactta gttgtgtccc gctgacgaac caatccgctc
     3301 gaccgtttaa gaaatcttgc accgacattg gttttttacc cgccggttga agttgggtaa
     3361 tattcagcac gccctgttgg gtggcaattt gaatcccggt tttatccaca ttaattaccg
     3421 tgccggctgc tttttcttgg tgaggtaaca ccgtggcttg gtgaattttg atactttgct
     3481 caacgccatc aacggttaag gttaaaaaac taaacggcca agggttaaaa gctcggatat
     3541 tgcgttccag ttgaactgct gggagttgcc aatccaattt agcttcttct ttggaaagtt
     3601 tctcggcgta attggacagt gcttcatctt gtttttccgc tttgaatgtg tggttggcta
     3661 aaccgtttaa cacctcaagc aatgccagag gggcaagttc tgccagtttg gcatacaagg
     3721 aggcggaggt ttcattcggc tcaatcgggg ttgtgacttt atgtaacata tcaccggtgt
     3781 ctaagccaac gtccattagc ataatggtta cgccggtttc tgtatcacct gcccaaatcg
     3841 aacgctgaat cggagctgct ccacgccagc gaggtagtag cgaaccgtgt acgtttaggc
     3901 aaccatattt aggagcattc aatactgctt caggtagaat taagccgtaa gctaccacca
     3961 ccatcacatc ggcatttagg gctttcagtt cagcttgagc ctcttcctta cgcagggatt
     4021 tgggttggta aacaggaatg ttgtgagctt cggccagctg ttttaccggg ctggcttgca
     4081 gttttttgcc tcgcccggca ggtttatccg gctgagtata aaccgcaatc acgttatgct
     4141 cggaatctaa taaggcttgt aaatgttgag cggcaaaatc aggcgtgccg gcaaaaataa
     4201 tattgagttt tgacatattt tttattcgtt caagttatca atgaaagact tattaaacca
     4261 ttaaacagca caaataaaaa gtggcttgaa tatggtatca ttgccacatt caagataaga
     4321 gaatcaaaca atggaatttt ttatccccaa agcggataca atttttgaag aagagattaa
     4381 aaaaagccga tttatcacct accttcgcca tactgagggt atggagcagg ccaaggcatt
     4441 ttggcaggag ctgaaaattc agcatccgca cgcacgccat tggtgttggg caacggtggc
     4501 aggcttgccg aatgattcgc aacaatacgg cttttcggat gatggagaac cgtctggtac
     4561 agcaggtaag ccaatgctca attatttgct tgggtcaggc ttgggggaaa ttacagcggt
     4621 ggtggttcgc tactacggtg gtatccagct tggcaccggc ggcttggtga aagcctatgg
     4681 caatggggtg caacaggcgt tattgcagtt ggagcggatt cgcaaagttt tgcgaaaaaa
     4741 ctaccgcttg caatgtgaat atgagcagtt taataccatt tctcatctca ttagcgagaa
     4801 agatattgtg attgtcgagc aactttttag cgataaagtg gatttgttgc tggcatttaa
     4861 cccgagcgag gttgaggcct ttgcagatga attaacccaa cgcttttcgg gcaagctcgc
     4921 attaataaaa gaagaaatct agtctatgca atttttttcc attattcgta ttgttggcat
     4981 tttggtgatg tgtttttcat tcacaatgct tgtgccggca acggtcgccc taatttatgg
     5041 cgatggcggt gggcgggcgt ttttagaagc cttcgctctg aattttatgg tcggtacgct
     5101 gctttggtgg ctgtgccgtg acaacaaaaa cgagctacgt tcccgtgagg gctttttgat
     5161 tgtagtactg ttttgggtgg tgctgggttt gctcggggct gtgcctttta ttattttgga
     5221 aaaccccgat ttaaacgtgg cggaatccat ttttgaatcc ttttccggct taactacaac
     5281 gggtgcaacc gtcatcaccg gcttagatag cttgcccaaa gcgattttat tctaccgcca
     5341 atttttgcaa tggctagggg gaatggggat cattgtgtta gcggttgcga ttattccgct
     5401 gttaggcatt ggtggaatgc aactttatcg tgcggaaatg cccggaccgc tcaaagagca
     5461 gaaaatgcga cctcgcattg cggaaacggc aaaagcgttg tggctgattt atctcagcct
     5521 gacgattctg tgtgcggtcg cattttggct cgccggaatg acggtgtttg atgccatttc
     5581 gcatagcttt tccacggttt cgattggcgg attttctacc cacgatgcca gcatcggcta
     5641 ttttaataat gcggcaatta gctatattac cgtgtttttc ctgctacttt ctgcctgcaa
     5701 ctttgcattg cattttgcgg tgattgatcg catacgagat gcacaatcac gcaaaaaagg
     5761 gggtatttta agccgcttgt attggggcga ttacgagttt cgttttttct tgatggtgca
     5821 gttagggctt ttcttggttt gtaccttggt gttagtgggt tacggttatt tcgataatac
     5881 ctctgttacg attgagcaag cgttgtttca atcggtgtcc atttccacca cggccggctt
     5941 tactaccaat gattttagcc aatggagttc gtttttaccc acgctgttgg tgctgtcttc
     6001 atttatcggt ggttgtgccg gctcaaccgg tggcggcttg aaagtgattc gggtgttgct
     6061 gctttattta caaagtaaac gtgaaatcca ccgctacatt caccccaatg tgattcaacc
     6121 gattaaatta ggcaaccacg ttttatcgga acgggtggtt gatggtattt gggcgttttt
     6181 ctcggcgtac tttttcgtgt tcgtgatctg ctggcttgct accatcgctt gtggaatgga
     6241 aacctttgat gctctaaatg cggtaattgc ctcactaaat aatctaggtc ctgcattagg
     6301 gagcgtgagc agcaatttca cccaagtgcc ggacagtgcc aagttagtgc tgacttttgc
     6361 aatggtatgc ggacgtctgg aagtgtttac cttgctggtg attttaagcc ctgttttttg
     6421 gaaagattaa atgaaaacct taattctcta ttttacgacc gatggtcaaa ccaaaaaaat
     6481 tgccgaacga ttggcggacg tgattactca cgaagtcgag ctgatttcgc taaaagatca
     6541 agcggtcgat tttgccgaaa aaattgcaaa tgccgaccaa attgtcatcg gtgcttcaat
     6601 tcgctatggg cattttaacc cgttggttta tcagtttgtg gaacagcatt atccggcatt
     6661 aaacgccaag aaaactgcct tttacggtgt gaatttgacc gctcgcaaag ataatcgaaa
     6721 cacgcctgaa accaatactt atgtgcgtaa atttttagct aaagtgaaat ggaaaccaac
     6781 ccacattgag gtaattgctg gggcattact ttacctacgc tataagccat tcgaccgatt
     6841 gatgattcgc tttattatga aaattaccaa aggcgaaacc gatacctcca aagaatatga
     6901 gtacaccgat tggcagcagg tggagaattt tggcaaatta ttagccaatc agttataatc
     6961 aaactgatct aaatcaaaaa aatgtaaatt tatttaaatg ttgtagaaat tttaaagatt
     7021 ttgattataa tcgccagccc acgttcttgc gaagaccgtg tttaatcatt gaatgaaagg
     7081 aaagccccct tgagtatttt aaaagagttc cgtgaatttg cggtaaaagg taatgttgtt
     7141 gatatggctg tcggtgtgat tatcggcggt gcattcggta aaattgtttc ttcattagtt
     7201 agcgatgttg taatgccacc tatcggttgg ttaatcggtg gcgtggattt caaagattta
     7261 gccattgaaa tcgccccggc aaaagaaggt gcggaagcag tgatgttaaa atacggtgca
     7321 tttatccaaa acgtatttga tttcttaatt atcgcacttg cggtattcgg tatggtgaaa
     7381 gtgattaact cactgaaaaa agctccagtt gtagaagaag ctcctgcaga gccaacagcg
     7441 gaagagaaat tattaactga aatccgtgat ttattgaaaa aataattaac tgattttgaa
     7501 acaagggcta ttttagaata tctagaatag ccctttgtgt tatggttaaa aagaaaaaaa
     7561 ccaacataaa gtcggttttt ttcatatctg ttcgatatta cgctaatttt ttgatttgcg
     7621 ctgctaattt agatttgtgg ttagccgctt tgttagcgtg gattaagttt ttagatgcca
     7681 tacggtctac aactttttgc atttcaacga aagccgtttg agctgctgct ttatcgcctg
     7741 ctgctactgc tgcatatact tttttgatga atgtacgcat cattgagcgt tggcttgcgt
     7801 tgtgttggcg gcgtttttca gattgaaccg cacgtttttt tgctgacttg atattagcca
     7861 aggtcaaact cctaaaatat ctgtgtaaaa attagatata aacaaaatcg ttgtttatat
     7921 tgcctatcga tagtttttaa ttcattttca acgagacacg tgcttgaaaa taacaccgtt
     7981 tttctgatag aaaacgatag ggcgagattt taacagt
//
LOCUS       DANLXQ010000115         8009 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.115, whole genome shotgun sequence.
ACCESSION   DANLXQ010000115 DANLXQ010000000
VERSION     DANLXQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.115"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(76..381)
                     /locus_tag="PXD94_001925"
     CDS             complement(76..381)
                     /locus_tag="PXD94_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL6286761.1"
                     /translation="MRKPAHPGQILLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
     gene            complement(390..665)
                     /locus_tag="PXD94_001926"
     CDS             complement(390..665)
                     /locus_tag="PXD94_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6286762.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
     gene            957..1187
                     /locus_tag="PXD94_001927"
     CDS             957..1187
                     /locus_tag="PXD94_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286763.1"
                     /translation="MNAQATLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            1569..1805
                     /locus_tag="PXD94_001928"
     CDS             1569..1805
                     /locus_tag="PXD94_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286764.1"
                     /translation="MSNQEFVKLGSVEPSTIDELQKEDIRQAILKSVSITPYYVGLEI
                     AVCQAISAINKFSDGCITEELLDTKNGDKYRKNN"
     gene            2112..2187
                     /locus_tag="PXD94_001929"
     tRNA            2112..2187
                     /locus_tag="PXD94_001929"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2145..2147,aa:Arg,seq:tct)
     gene            2374..2880
                     /locus_tag="PXD94_001930"
     CDS             2374..2880
                     /locus_tag="PXD94_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286765.1"
                     /translation="MKTKYKIIIERAKQQGLTHIELTQKHCLYLATSVGKLCYGVANN
                     AEQHIKLAAGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELNGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            2884..3243
                     /locus_tag="PXD94_001931"
     CDS             2884..3243
                     /locus_tag="PXD94_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286766.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            3240..3719
                     /locus_tag="PXD94_001932"
     CDS             3240..3719
                     /locus_tag="PXD94_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286767.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKAVNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFIPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNVLAEMVKRTQEANNAKHA"
     gene            3853..4065
                     /locus_tag="PXD94_001933"
     CDS             3853..4065
                     /locus_tag="PXD94_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286768.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFILAEENIIELLENNGFETIAT
                     ILAGSDGWRNAINAYLAEQNKHRNEF"
     gene            4078..5028
                     /locus_tag="PXD94_001934"
     CDS             4078..5028
                     /locus_tag="PXD94_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286769.1"
                     /translation="MTTANQQFELILKTESRVVSTNLQTFEEQANQYLATLTTTFETD
                     DDFAKAKEEVKELESIEKKIRDAIKQTQTGEIAELVLTAEQIAERFRQERLTRDKLVK
                     SKETEIKKQIADKAIEEITDTRNKLVKVSDISLALEITMPKHGIANRIAEAQKNKRTI
                     DSLTKAVNAEKALIISEMSVEIGRLTERLEQLAAKSAYLFPDAVKLIATQDDLAPIIQ
                     QRLAEEAEREAAIKAKAEADKVQAEAKAITDKMERKQAVENPQNVAKMNIEEPAQTDG
                     PLGDFIITIRLNQTIQTNAVSIARELKAKFGDSVSLNKAK"
     gene            5069..5863
                     /gene="bet"
                     /locus_tag="PXD94_001935"
     CDS             5069..5863
                     /gene="bet"
                     /locus_tag="PXD94_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDL6286770.1"
                     /translation="MTTALQTLTNKLAERFEMGDGSGLVETLKSSAFAGATVSDAQMI
                     ALLVIANQYQLNPWTKEIYAFPGGNGGLTPIVGVDGWVRIINREPQYDGMEFHFTDDY
                     SACTCTIYRKDRSKPIVVTEFMGECKKSSPAWNSHPKRMLRHKAMIQCARLAFGFTGI
                     YDQDEAERIAENEKPPKNITPQNNVVETTAVELISEEQLSQIRQLMQVTGTEEAKILA
                     YIGVQALNQIPKSQAEAVIKKLNLTLDKQNAEKADNGESVGEEIPL"
     gene            5860..6495
                     /locus_tag="PXD94_001936"
     CDS             5860..6495
                     /locus_tag="PXD94_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL6286771.1"
                     /translation="MTGDLIILDCEQGSEEWLNARLGIPTATGIENIVTPSGKKSSAW
                     NSYLAELVAESIEGAKDGFKSSDMARGNELEPLARMAYEFETDTEVVQVGGVYLNAEK
                     QLMVSPDGLIPNLKKGLEIKCPKMKTHIKYLLEGGVPNEYLIQVQSALWVTGYETWDF
                     VSYCPEYQKQPLYIYTAERDPVLMKAFDQYIPQFIKSLYALKGVTAPKGAF"
     gene            6508..6684
                     /locus_tag="PXD94_001937"
     CDS             6508..6684
                     /locus_tag="PXD94_001937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286772.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAICHFRRTAEYSARATHA
                     TAAECYGGIACTST"
     gene            6705..7157
                     /locus_tag="PXD94_001938"
     CDS             6705..7157
                     /locus_tag="PXD94_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL6286773.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVGMWEEPRPAVTLTLPCPLKEPQENM
                     WFIDNDFTIVKSMFANAPFVKKFLPQGRCFASEEDAQEWLDAMRNSRR"
     gene            7235..7762
                     /locus_tag="PXD94_001939"
     CDS             7235..7762
                     /locus_tag="PXD94_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL6286774.1"
                     /translation="MNEEKYFSVDVSDETHVIRLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERASELFGEAEQVIEPPTLLE
                     NNGWISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIA
                     VTHWQPLPEPPKEEK"
     gene            7759..7962
                     /locus_tag="PXD94_001940"
     CDS             7759..7962
                     /locus_tag="PXD94_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286775.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
BASE COUNT         2791 a         1603 c         1614 g         2001 t
ORIGIN      
        1 atattccttg aataaaccgc cctcgtggcg gttttctttt cctaagaaca ataaaatcct
       61 ccgaagaggg cattattaag cgtgagcagt ttctccataa tttccaccaa aatgaaaagg
      121 cataacagga tttaaccgct gctgctttaa ctcccataaa tcatatttag tttgtaagtt
      181 tagccataac ttagccgtac taattcctgc ctcttctaaa cttaacgcta aatttgcagt
      241 cattggtgtt ttgccgtgta atactcttga taaagtttca cgagaaaacc caagatgatc
      301 tgctaattct ttaattttga tgttatttgg ttcaataaat ccatctaata aaatttgccc
      361 cggatgtgct ggtttacgca taactccccc taatgataat cttcataatt caaaatataa
      421 gcatcgccat ttacaaattc aaatgtaatc cgccagttgc cattaaccgt catagaataa
      481 atgccttttc gatttccttt taactcgtgg cattggtaaa aaggcataaa ttcatcaata
      541 ctttctgctg aatcaatcaa atctaaaata ccatcgattt tacgttgatg attaagctga
      601 ataccttttg ttattccttt ttcaaaatat tgttttaagc ctttatgctt gaagctctta
      661 atcataataa cacctagcaa acacaatgtg atacaaatat atcacgcaca ataaaaaaga
      721 caatcttttt taagtgcttt ttctcctaat tgtctttctc ttcttatgtt aagtgtttaa
      781 aaaacaagca atcaaacaac ctttctaaaa ataatttctc tttaaaatca attatttaat
      841 cacgaattga aatttgtttg gctaaaataa ttacaaattg aattgactta atgatttcaa
      901 tttgtgataa taaacccatc aaaacaaaca acgccacaga caaagaggaa accaaaatga
      961 acgcacaagc aacgctaaat aaccacaaag actacatttt atgcggacgc aaagaaaaac
     1021 gcaccagtga tttcatcaat gtgtttgagg tctttgaaaa tgaagccacg caagagtttg
     1081 tgattgaaag agcaatgttc agaaatggca agttaatcga ctggaaccaa agcgacaaaa
     1141 tgaacgccga gcaagctcaa caactttggc aagcctacat tcactaagaa tttttatcaa
     1201 agcccttcac ggagggcttg aataaaggtt ctaaacctcg ccgaaaggca ttgttcttta
     1261 aaaattaaac caaaaacaca tcacgacaag cggattactt agcaatgtaa tgacattgga
     1321 gagacagaac caatctttgc ataaagcggt aaaacgtatg cggtaaatga acttataggc
     1381 aagttgtgaa actctctaag ctactcggac tgaaagaata gtacggtagt tgtggcgaat
     1441 ttactggtga tgtgcgaact agcggaaagg ttaagcactg gcagtgaatc gggaagcaca
     1501 aacgctggtt caattccaaa gcatatttga gaaagtgtgc tttggaatat taataacagg
     1561 agaacaaaat gagtaatcag gaatttgtta aattaggcag cgttgaacct tcaacgattg
     1621 atgaattaca gaaagaagat attaggcagg caatattaaa atccgtctct ataacgccat
     1681 attatgtggg gctggaaata gcggtttgcc aagcaatatc agccatcaat aaatttagtg
     1741 atggctgtat tacagaagag cttttagata cgaaaaatgg tgataaatac cgaaaaaata
     1801 actaatcaat aacaccaaag tgatttcatt ttttcggcat tagccctaga tgttgcccaa
     1861 gtattatatt cctcgaagtt gtggcaaaca agaggacttg gcccggtggc tcacaagtat
     1921 aaaaaagagc caccatctct aacagctctc aacagagagt gagacaaata accccgtttt
     1981 agttttaatg ttttctaaag tcggcgttag gcagcgaaac gaatgtacta gagagttgtt
     2041 agagatggtt agtccatcac attccttaga ttgaagtaag ccctgccgtt ggggttaagc
     2101 aacggtactt tgaccgcata gttcagtgga tagagcagct gccttctaag cagtgggtcg
     2161 agagttcgaa tctctctgcg gtcgccatcc aaaaccgctt tacgcacatc atcgatgacg
     2221 agcctacgaa gtggaaacct accgtgtaga gcggttttga atggctaagt tgtgtaactg
     2281 ctacaactta aaaagagcgg ttgataataa acataatgac tccgtttccc cgcctcgtgc
     2341 ggggcttttt ttatccacat aaaaggattc aaaatgaaaa ctaaatacaa aattattatt
     2401 gagcgagcaa aacaacaggg tttgacccat attgagctta ctcaaaaaca ttgtctgtat
     2461 ctcgctacat cagtagggaa actgtgctac ggggtagcta ataatgcaga acaacatatt
     2521 aaacttgctg ctggagagat ttttacacta ctaacgatca ttaaccatca aatccagttc
     2581 gatgaagact tagagcttca cacgtggaaa gctagtggaa caaccccacc cgagcaaaga
     2641 gcgatacgta tgttacatca tacctcgcaa tttgcttggt ttgctccttg gttagtggac
     2701 agcaatgaaa aagaacaaaa ccaaaataca atcaggatta atttatttaa caaatgctct
     2761 gggattctga ttgaactaaa tggcattctt aaagaatacg agctaactct tgatgagtgc
     2821 ttagatgaat tcttgaaaaa taagcattgt ttgaatgaat ttagtattca caaggagtaa
     2881 aaaatgacga cgtttagtat ttataccagt gtatggcacg acagctccac gaaaggcttt
     2941 cgccacaaca ttaagcataa acgccacgat tgctggagag cggatatccg cataaagcaa
     3001 aaaatcaatg atgtgtgggt gcagatttca aggatacgca aacgcttcaa gtgccctgaa
     3061 caggcaaaat cctttgtaga acaatggaaa caaattgatt cacctgcatt tatgcaacac
     3121 caaatcaacc gccaagcatt aagagaagaa ttatcaaacc ttgagcaacg caaacagaag
     3181 caagacggaa tacttgaggt gattgagtat cgtattagcc aaatcaagga gttgctgaaa
     3241 tgaaacgcaa taaagtagaa agctatacta tccgcttatt aaaaaataat catacaaaac
     3301 gctacttcgg agaagttata ttaaacggca taaaagaaat cagcacggca gagatgatgt
     3361 ctgaaattaa cgctattaag gcagtaaata agcgcattga agctctgaat aaaaccaaag
     3421 ccgttaattt gcctaattat cctgaaatcc ggggtcatca gcctattatg tacaattaca
     3481 attttgaaca aattgaggaa gatcctgaac aggttaagcc taaaccatca aaaccaaaac
     3541 gcaagccatt cataccttat gggctaaacg gctactttgt ggatagcaac ggtaatattc
     3601 gcttacatct agacagacga gcaagtgccg aaacaattac ccttgaaact gatttcttca
     3661 acgttcttgc cgaaatggta aaacgtacac aagaggcaaa caatgctaaa catgcttaga
     3721 caatggatta aatacggctt atacgccctg ctttttatgg ttgccatcac cctcctaatg
     3781 caatgaagag cttatccctg aaagcacctg caagcctgaa acttgcgaat ttaaagtaac
     3841 ggagaaagtg tgatgatagg atacgaagac tacacagatg cttcacttca accagaagaa
     3901 gaccctagcg aagaggcttt catcttggct gaagaaaaca tcatcgagct attagaaaac
     3961 aacggctttg aaaccattgc caccatccta gccggttctg atggctggcg taacgcaatc
     4021 aacgcctacc tagccgaaca gaacaaacac agaaatgaat tttagaggaa taagaaaatg
     4081 acaactgcaa atcaacaatt tgaacttatt ttaaaaaccg aaagccgagt ggtttctacc
     4141 aacttgcaaa cttttgaaga gcaagcaaat caataccttg ccacgctcac caccaccttt
     4201 gaaaccgatg atgattttgc aaaagcaaaa gaagaagtga aggagcttga aagcattgag
     4261 aaaaaaattc gtgacgcaat taaacaaacg caaactggcg agattgcgga attggtatta
     4321 acagcagaac aaatcgctga acgtttccgc caagagcgat taacccgaga caagctcgtt
     4381 aagagcaaag aaacggaaat caaaaaacag attgccgata aagccattga agaaatcact
     4441 gataccagaa acaagctggt taaagtaagt gatatttccc ttgccttaga aatcacaatg
     4501 ccaaaacacg gcattgctaa tcgtattgcc gaggcacaga aaaataaacg cacgattgac
     4561 agcttaacca aagccgtgaa cgccgaaaaa gcgctgatta tcagcgaaat gtcggtagaa
     4621 atcgggcgct tgactgagcg attagaacag cttgctgcaa aatcagctta tctttttcct
     4681 gatgcagtga aattgatcgc aacccaagac gacctcgccc caattattca acaacgcctt
     4741 gccgaagaag ccgagcgtga ggcggcgatt aaagccaaag cagaagccga caaagtacaa
     4801 gccgaagcca aagcgattac tgataaaatg gagcgcaaac aagcggtcga aaatccgcaa
     4861 aatgtggcaa aaatgaacat cgaagaaccg gcacaaaccg atgggccatt aggcgatttt
     4921 atcattacta ttcgcttaaa ccaaacaatc caaaccaacg cagtcagtat tgctcgtgag
     4981 ctgaaagcga aatttgggga tagcgtttcg ctcaataagg ctaaatgatt tgataccgca
     5041 caaattcaac ctaaataagg atcatattat gacaactgca ttacaaacac taaccaacaa
     5101 acttgccgag cgttttgaaa tgggagatgg ctcaggatta gttgagacac ttaaatcgtc
     5161 tgctttcgca ggggcaaccg taagcgatgc tcaaatgatt gcactattgg ttattgctaa
     5221 ccaataccaa ttaaaccctt ggacaaaaga aatttatgca ttccccggtg ggaacggtgg
     5281 attaacgcct attgtcggcg ttgatggttg ggttcgcatt attaaccgtg agcctcaata
     5341 tgatggaatg gaatttcatt ttaccgatga ctattccgcc tgtacttgta ctatctaccg
     5401 taaagataga agtaaaccga ttgtggtgac ggaatttatg ggggaatgta aaaagagctc
     5461 tccagcttgg aactctcacc caaaaagaat gttacgccat aaagcaatga tccaatgtgc
     5521 aagattagcc tttggcttta cgggtattta cgaccaagac gaagcagagc gtattgcgga
     5581 aaatgaaaaa ccacctaaaa acatcacacc acaaaataac gtggtagaaa ccactgccgt
     5641 tgaactcatt tcggaagaac aactgtcgca aatccggcag ttaatgcaag taaccggtac
     5701 ggaggaggcg aaaattctcg cctatattgg cgttcaagca ctgaaccaaa tccctaaatc
     5761 gcaggcagag gcagtgatta aaaagctcaa tcttacgcta gacaagcaaa acgctgaaaa
     5821 ggcggataat ggcgaaagtg tcggtgagga aatcccacta tgacaggcga tttaatcatt
     5881 ttagattgcg aacaaggatc agaagaatgg ctcaatgcca gattgggcat tcccaccgcc
     5941 acaggcattg aaaatatcgt tacgccaagc ggtaaaaaat cgtccgcttg gaatagctat
     6001 cttgccgaac tggttgccga aagcattgag ggagcaaaag acggttttaa gtcgtctgat
     6061 atggcacgag gcaacgagct tgaaccgctt gcacgaatgg cttacgagtt tgaaaccgac
     6121 accgaagtcg tgcaagtcgg cggtgtgtac cttaacgccg aaaaacagtt aatggtaagt
     6181 cctgacggct taattcccaa tctaaaaaaa gggttggaaa tcaaatgccc gaaaatgaaa
     6241 acccacatca aatacctgct agaaggcggt gtgccgaatg aatatcttat tcaagtgcaa
     6301 tctgcccttt gggtaacagg ctacgaaacg tgggactttg tcagctactg ccccgaatac
     6361 caaaaacaac cgctttacat ctacacagcc gaacgtgatc cggtattgat gaaagccttt
     6421 gatcaatata tcccgcaatt tatcaaatcc ctttatgcat tgaagggggt aaccgccccg
     6481 aaaggggctt tttaataggt agccaatatg acaacccaaa agaaatacga actactccaa
     6541 aacgacacaa tcaaccacaa cggcagaaca ctctaccgca tcaaagccat atgccacttt
     6601 aggcggacgg ctgaatattc cgcaagagcc actcacgcca cagccgctga atgctatggt
     6661 ggaattgcct gtacatcaac ataaccacaa acacggagaa taaaatgaaa ccatttgatt
     6721 taaataaagc attggcaggt gagccagtaa aactaagaaa taatgataag gcatttgtaa
     6781 aatacctaat tagtgatgac tatattagag ataataaaga ccaccaagta caagggtata
     6841 cagttgatga agaaaatgta tttttgtcag aagtatcgtg ggctgttagc ggatctcatt
     6901 tcaatgatgg aacaatagct caatatgata ttgttggtat gtgggaagaa ccaagaccag
     6961 cagtaacatt gacgttacct tgcccattaa aagaacctca agaaaatatg tggtttattg
     7021 acaatgattt taccatagta aaatctatgt tcgcaaatgc cccctttgta aaaaaattct
     7081 tgccacaagg gcgatgtttc gcttcagaag aagacgctca agaatggtta gatgcaatgc
     7141 gaaatagtcg taggtaaatc ggcagcacta acagctgaat gctgctgttg aatttcttgt
     7201 atatccacat aaacacggaa cgcggagaaa gaaaatgaat gaagaaaaat atttttcggt
     7261 cgatgtctcg gatgagacac atgtaataag gttgcacgaa acattagagc aagcaaaaca
     7321 aagctgttta aatggtgcta ctgaagccta tgagtttgcg ggtgacatgg atgatcacga
     7381 aagctatgaa agctatgaag cctatgattt accctatgca gtttatggtg ttgttttagg
     7441 tagagcaaaa tcagatattc gtccactaac agacgaagaa agagcatcgg agttatttgg
     7501 ggaagctgaa caagttattg aaccaccaac acttttagaa aacaacggct ggatttccat
     7561 tgaggacaaa ttaccaccta ttgaaacgga tgtgctaggg ttatgcgata tttcaggaat
     7621 gcagttaatt ttaatagttt caagagaact tgccgataac gagtggtatt ttcttagtgt
     7681 aaatcaatat gggcttgatg acgatgtgat tgcagtaacc cactggcaac cacttcccga
     7741 accaccaaag gaggaaaaat gaccgacacc gaacgcttag actttatcgc ccaacgcaaa
     7801 ttagatgtcc gatattctga tttggtggac gaatttatta ttgctagcta taccaaaccg
     7861 cacgagcagt attttcattg ggcaagaaat gccgatttac gcacggcgat tgatgaagca
     7921 atgaaagaaa tgagaggact aaacaatgca acaactaatt aaaaacatcg aacaatgggc
     7981 ggaagaccgc aatttaatta acggctcaa
//
LOCUS       DANLXQ010000116         7883 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.116, whole genome shotgun sequence.
ACCESSION   DANLXQ010000116 DANLXQ010000000
VERSION     DANLXQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.116"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            20..1006
                     /gene="pheS"
                     /locus_tag="PXD94_001941"
     CDS             20..1006
                     /gene="pheS"
                     /locus_tag="PXD94_001941"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL6286776.1"
                     /translation="MQHLKDLTNKAREALDKIDQNLEALEEFRVEYFGKKGHFTTLMQ
                     ELRNIAAEERPAIGAKINEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKT
                     ELGGLHPVSITIERVVKFFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFW
                     FDANRLLRTQTSGVQIRTMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKE
                     ANFTELKGLIHDFLKAFFEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMV
                     HPNVLRNVGIDPEEYSGFAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            1054..3441
                     /gene="pheT"
                     /locus_tag="PXD94_001942"
     CDS             1054..3441
                     /gene="pheT"
                     /locus_tag="PXD94_001942"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL6286777.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFAPLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEAGEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTSIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            3499..3795
                     /locus_tag="PXD94_001943"
     CDS             3499..3795
                     /locus_tag="PXD94_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL6286778.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            3834..4355
                     /locus_tag="PXD94_001944"
     CDS             3834..4355
                     /locus_tag="PXD94_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6286779.1"
                     /translation="MKLTKRWLSLGCLLAITAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            4371..5414
                     /gene="degS"
                     /locus_tag="PXD94_001945"
     CDS             4371..5414
                     /gene="degS"
                     /locus_tag="PXD94_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL6286780.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            5423..6097
                     /gene="cmk"
                     /locus_tag="PXD94_001946"
     CDS             5423..6097
                     /gene="cmk"
                     /locus_tag="PXD94_001946"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL6286781.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRSVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIKF"
     gene            6189..7856
                     /gene="rpsA"
                     /locus_tag="PXD94_001947"
     CDS             6189..7856
                     /gene="rpsA"
                     /locus_tag="PXD94_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL6286782.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
BASE COUNT         2465 a         1287 c         1844 g         2287 t
ORIGIN      
        1 tgagtttaga ggataggtaa tgcaacacct aaaagattta actaataaag cacgtgaagc
       61 tttagataag attgatcaaa atttagaggc tttagaagag tttagagtcg aatattttgg
      121 taaaaaaggg cattttacaa cgttaatgca agaattacgc aatattgcag cggaagaaag
      181 accggcaatc ggagcaaaaa ttaatgaagc aaagcaagtt gtgcttgatg tgttaaatgc
      241 caaaaaagaa gaatgggaac aagctgtatt aaatgcaaaa ttagctaatg agagcattga
      301 tgtttcactg ccggggcgta aaactgaatt aggtggtttg caccctgttt ctatcactat
      361 tgagcgtgtg gtgaaatttt tctctgaatt aggttttacc gtagcaaacg gacctgaaat
      421 tgaaaccgat tactataact tcgatgcgtt aaacattccg gatcatcatc cggctcgagc
      481 ggatcacgat acgttttggt ttgatgccaa tcgtttatta agaacccaaa cctcgggtgt
      541 gcaaatccgt acaatggaaa atgtaaaacc gcctatccgt attgtggcac cggggcgtgt
      601 atatcgtaat gactacgacc aaacccacac accgatgttt caccaaatcg aattgttata
      661 tgtggataaa gaagcaaact ttaccgagtt aaaaggctta attcacgatt ttctcaaagc
      721 attctttgaa gaagatttac aagtgcgttt ccgcccgtca ttcttcccat tcactgagcc
      781 ttctgcagaa gtagatgtaa tgcgtcaaaa cggcaaatgg ttagaagtgt taggttgcgg
      841 tatggtacac ccgaatgtgt tacgcaatgt agggattgac cctgaagaat atagcggttt
      901 tgctgtggga atgggcgtcg agcgtttaac gatgttacgc tacaatgtca cggatttacg
      961 ttcattcttt gaaaatgact tacgtttctt aaaacaattt aagtaataca agcggttaaa
     1021 aatttagaaa attttacaaa taggatagaa ataatgaaat tcaatgaatc ttggttgcgt
     1081 gaatgggtaa acccagcaat tagcacagaa cagttatgcg accaaattac aatgttaggg
     1141 ttagaagttg acgatgtaga gccggtagct ggtgcgtttt cgggtgtagt tgtgggtgaa
     1201 gtggtggaat gcgcccagca cccggatgct gacaaattgc gtgtcactaa agtgaatgtt
     1261 ggtggcgaac gtttattaga tattgtttgt ggtgcaccaa actgccgtca aggtttaaaa
     1321 gtggcttgcg ctgttgaagg tgcagtgcta ccgggcgatt tcaaaattaa gaaaaccaaa
     1381 ttgcgtggtc agccttcaga ggggatgtta tgttcttact cagaacttgg gattaaagaa
     1441 gatcatagcg gtattattga attacctgct gatgcaccta ttggtcaaga tttccgtgaa
     1501 tatttaaatt taaatgatgt ggcgattgaa atcagcctta cgccaaaccg tgccgactgc
     1561 ttaagcattg ccggcattgc tcgtgaagtg ggggtgatta accgtgtcga tgtgaaagct
     1621 ccaactatta cagatgtaaa agccacgatt tcagataaag tttcagtgga attacaagca
     1681 ccggaggctt gccctcgcta tttagcccgt gtggtgaaaa atgtgaatgt caaagcgaca
     1741 tcgccacttt ggttacaaga aaaattacgc cgttgtggga ttcgctcaat cgatccaatc
     1801 gttgatatta ctaacttgag ccttttagaa ttagggcagc caatgcacgc ctttgatgca
     1861 gcgaaaattg aaggtggcat tcaagtgcgt atggcaaaag aaggcgaaga attggtattg
     1921 ctagatggca caaacgctaa attacaatcg aataccttag tgattgctga tagcaaaggg
     1981 gcattggcta tggcaggtat tttcggtggc gaagccagcg gggtgagcga gaatacgaaa
     2041 gatgtggtgt tagagtctgc attttttgct ccattagcga ttaccggtcg cgctcgtcaa
     2101 tatgggttac ataccgacgc atcacaccgt tttgagcgtg gggttgatcc agaattaact
     2161 cgtcaagcaa tggagcgtgc aacggcatta ttactggaaa tttgcggcgg tgaagcaggt
     2221 gaaattgtgg aagccgtgag ccaagaaaac ctaccaaaac gcaatactgt cacgcttaga
     2281 cgcagtaaat tagattcggt gattggtcat catattgacg atgcaacggt aaccgatatt
     2341 ttaacccgct taggcttgaa cgtaacattt gcggataata gttggacggc aattgctcca
     2401 agttggcgtt ttgatattga aattgaagaa gatttaattg aagaaatcgc gcgaatttat
     2461 ggctacaaca gtatcccaaa taattcgcca cttgctcatt taacgatgaa aggtacgccg
     2521 gaaaaattac tggaagcaaa ccgtattcgt atggcattgg tagatagtga ttaccaagaa
     2581 gtgattactt atagctttgt tgatccgaaa aaacaatcat tattgcaccc acagcaagaa
     2641 gccttgattt taccaaaccc gatttcaagt gaaatgtcgg caatgcgtgt ttctctttta
     2701 accggtttgt tggacactat cgtgtataac caaagccgtc agcaaaaccg tgtgcgtatt
     2761 tttgaagcag gtctacgttt tattccggat agcaatgcgg aatcaggcgt gcgtcaagaa
     2821 tttgtttttg gtgcggcgat tgtaggcgat aaacgtccgg tacattggga aaacaaatca
     2881 gaagcagccg atttctttga tttaaaaggc gatattgagc gtattctttc tttaacctct
     2941 atccgccacg atctgcaatt tgtcgcaaaa cagttcccag cattacaccc ggggcaatcg
     3001 gctgcgatta tgcttgatgg caaagaaatt ggttttatcg gaactgttca cccgtcaatt
     3061 gtgcaaaagt ttggcattaa aggcaaaccg attgtatgtg aaattctagc atcagctatt
     3121 gcagagcgtc cggttacaca agcgggcgaa atttctaaat ttcctgcaaa taaccgtgat
     3181 attgcggtag tggttgatga atctgttcct gcaggtgagg tgttagaggc ttgtcgtaaa
     3241 gctggtggta ataaattagt ggcagtaaat ttatttgacg tttaccgtgg ttcgaattta
     3301 gaaagtggta agaagagttt agccattagc ttaacggttc aagataatga aaaaacatta
     3361 gaagaaaatg aaatcaatgc ggtaattcaa gcggtattag ctgaattagc aacacgtttc
     3421 aatgcggtac ttcgtgatta atcagtaaaa tatggctgat ttattttatc agccataaca
     3481 ataaactaag gataaatgat ggcgcttacc aagattgaaa ttgcagaaaa cctgattgaa
     3541 aaatttgggt tagaaaagcg tgtagcgaag caatttgttg aattgttctt tgaagagatc
     3601 cgatcctccc tcgaaaatgg tgaagaagtg aaattatctg gttttggtaa tttttcactg
     3661 agagaaaaaa aagctcgtcc aggaagaaat cctaaaacgg gggaaaatgt ggctgtttca
     3721 gcccgtcgag tagtagtttt taaagctggg caaaaattac gagaacgtgt tgagcaagct
     3781 agctcccaat cataggggca gttatattag ttagatttaa tgtggagtaa taaatgaaat
     3841 taaccaaacg gtggctttcc ttaggttgtt tgttagctat tacagcgatg ttagtggctt
     3901 gttcaagttc agatacaacc tctaagtctc aagtaaaaag taaagtttat cgttcaatat
     3961 accaatcaga cctttctgat cctattatgg caattagtcg attaagtgaa catcaacacg
     4021 aatggaaagg cactcgctat cgtttaggtg gcaatagcaa ggctggcatc gactgttctg
     4081 gttttatgca agtcactttt aaagatcttt ttgggataga tttgccgaga accaccacag
     4141 agcaggctaa ggctggtgag cgtatatcca aagacgaatt gcgtacaggt gatttagttt
     4201 ttttcaagac agggcgtggt ccaaatggga aacacgttgg tgtttatgta aaaaatgggc
     4261 aatttcttca cgcctcaaca aagggagggg tgatttattc tgatttggat tcgccatatt
     4321 ggtctaaggc tttctggcag gctcgtcgtt tataatttag ggtaacatac atgctgaaaa
     4381 aaatcggaca agcggtcata tttggacttt tttgtgcaat tttaattttg tttgtagctc
     4441 caattattca gagtaaaggg agttcgtttt cattatcagc attcactcaa aaagagattg
     4501 taagctacaa tgcagcggtt aaaatagcct cccccgcagt cgttaacgta tatagccgaa
     4561 attttagttc tgaaaatgaa acatttgaag ttaagaattt aggttcgggt gtgattatga
     4621 gtagcgatgg ctatatttta actaacaggc atgtgattga agatgcggat caaatcgtga
     4681 ttgctttgca aaacggtgct atttcgacta atgtaaaatt agtcggcgat gattctttaa
     4741 ctgatttagc tgttttaaaa attgagggtg agaatttacc tactattcca caaaatccgg
     4801 agcgcaaact acaaattggc gatgtggtgt tggcaattgg taatcctttg aatttaggac
     4861 agagcattac gcaagggata gtcagtgcta ttgggcgaaa aaccttaaca gaaagtggta
     4921 gacaaaattt tattcagaca gatgtttcca ttaatcaagg gaactcaggt ggtgctttaa
     4981 ttaatactgc tggtgagcta ataggtatta acactttgcg ttttgggaaa aatacgaatg
     5041 aattgtcaga gggcctaaat tttgcgattc ccattaatct ggcgaatcaa attatgagta
     5101 agatcattaa agatggccga gttattcgtg gctattttgg ggtaagtaca gatttgcttg
     5161 acaccgcaaa aatgcaaggt tatgaaaaag gggtgatcat tgttaatgta gataaaaatg
     5221 ggccagccgc aaaagcgggg attcaagcag gtgacttagt gcttaaagtc ggagatattg
     5281 aggcattctc tccttctcaa atgatggaag agcttgctga aatgaaacct tatactacag
     5341 taaaagtatt ggttcaacgt gatgaacaat tacttaactt tgatgtaact attactgagt
     5401 tacagactca atagggtaga aaatgaaaaa aatagtgatt acagttgatg ggccgagtgg
     5461 tgcaggtaaa ggaacgttat gtcatgcatt ggctgaaaaa ttagggtttg attttttaga
     5521 ttctggagcc ttataccgga ttactgcatt aagcgcaatt aaaaaagagg ttccccttga
     5581 tgatgaggaa aaactagcgg aagttgggcg taatcttgat attcaatttt tgccagaaaa
     5641 tggcgagatt caggtgattt tggaaggaga aaatgtaggc gacttaatcc gaacggcaga
     5701 agcaggtcaa aatgcctcta aagtcgctgc tttccctttg gtacgtgaag cacttttaga
     5761 acgccaaaga gcctttagta gcgaaaaagg tctgattgca gatggacgag atatggggac
     5821 agttgtgttt cctgatgcac aaattaagct gtttttagat gcaagtgccg aagaacgcac
     5881 aaaaagacgt gtaaaacagt tgcaagaaaa gggatttaat gctaactttg acgagatttt
     5941 agccgagata aaagaacgtg attttcgcga tagaaatcga tcagtcgcgc cacttgttcc
     6001 ggctaaagat gcgttgctgt tagattcaac gcatttatca attgaagatg tgattaacca
     6061 agcattaaat catatcgctc aatacattaa attttagttt gttgcttcac ataggatttg
     6121 tgtagctttt attatcaacc ccgtttaaca ggacgttaga tggatgttta tttttaagaa
     6181 gatttattat gtcagaatta tcgtttgctc aactacttga agaatcattt gcagctacac
     6241 cacgtttagg tgatgttgtt aaaggtactg ttgttgctat ccaaaaaggc ttagttattg
     6301 ttgatacagg tttcaaatct gaatctgcaa tcgatgcctc agaatttact aacgcacaag
     6361 gtgaattaga agttcaagtg ggtgatgaag tagatgttgt tcttaaagct gtagaagacg
     6421 gttttggtgc aacagtagtt tctcgtggtg atgcaaaacg taacgaagct tggattgctt
     6481 tagagaaagc atttgaagaa caagctacag ttatcggttt cgtaaatggt aaagtaaaag
     6541 gcggtttcac tgttgaatta aacggtgttc gtgcattctt acctggttca ttagttgata
     6601 ctcgtccggt tcgtgattct ttaaacctag aaggtaaaga gtacgaattc aaagtaatca
     6661 aattagatca aaaacgtaac aacgtagtag tatctcgtcg tgctgttatc gaaacagaaa
     6721 gcaaccaaga tcgtgatgcg gtattagcaa acttagttga aggttctgaa gttaaaggta
     6781 cagttaaaaa cttaactgac tacggtgcat tcgtagattt aggtggcgtt gacggtttat
     6841 tacatatcac agatatggct tggaaacgtg ttaaacaccc aagtgaagtg gttaatgtag
     6901 gcgatgaagt tactgttaaa gtattaaaat ttgacaaaga caaaactcgt gtatcattag
     6961 gcttaaaaca attaggtcaa gatccttggg ctgtaattgc tgaaaaccac ccggtaaaca
     7021 gcaaattaac tggtaaagta actaacttaa ctgactacgg ttgcttcgtt gagattttag
     7081 acggtgttga aggtttagtt cacgtttcag aaatggattg gacaaacaaa aacattcacc
     7141 catctaaagt ggttaacgta ggcgatgtag ttgaagtaat ggtacttgaa gttgatgaag
     7201 aacgtcgtcg tatctcatta ggtttaaaac aatgcaaagc aaacccttgg gaacaatttg
     7261 cggcaactca cagcaaaaat gataaagtat caggtaaaat caaatcaatt actgacttcg
     7321 gtattttcat cggtttagaa ggtggtattg acggtttagt tcacttatct gacatttctt
     7381 ggaatgtttc aggtgaagaa gcagttcgta actacaaaaa aggtgatgaa gtagaagctg
     7441 ttgttctaca agttgatgca gtgaaagagc gtatctcttt aggtatcaaa caattagaat
     7501 ctgatccttt cacaaacttt gtagatggta ctaaaaaagg tgcaatctta acaggtaaag
     7561 ttgtagaagt tgatgctaaa ggcgttaaag ttgaattgga aggtggcgtt gaagcattta
     7621 ttcgtgcaaa cgaagcaact gctgagcgtg tagaagacat cacttctgtt atttctgttg
     7681 gcgattcaat cgaagcgaaa tacaccggtg ttgatcgtaa atctcgcgta gttaacttat
     7741 ctgttcgtgc aaaaaacgaa gctgaagaat cagcagcaat tgcacaagtg aacaaagagg
     7801 aagttgttgt acctaatgca tttgctgaag ctttcaaagc agctaaaggc gaataattag
     7861 tatagactaa tctaaaatgg gta
//
LOCUS       DANLXQ010000117         7832 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.117, whole genome shotgun sequence.
ACCESSION   DANLXQ010000117 DANLXQ010000000
VERSION     DANLXQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.117"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            33..554
                     /locus_tag="PXD94_001948"
     CDS             33..554
                     /locus_tag="PXD94_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL6286783.1"
                     /translation="MVKFATLQLADKDLAEDVVQDALTNAYKHSASFRREAALKTWIF
                     AILKNKILDLLRTRKRHIPVSSLCEEEESPSAFFDRHDHWLEHQDVTEWRGVAQSTYQ
                     KEFWAIFDICLNKLPAQQARVFMMREHLEMSSAEICQECEISLSNLNVLLHRARLRLQ
                     ECLSRNWFREESK"
     gene            551..736
                     /locus_tag="PXD94_001949"
     CDS             551..736
                     /locus_tag="PXD94_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL6286784.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            766..1050
                     /locus_tag="PXD94_001950"
     CDS             766..1050
                     /locus_tag="PXD94_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286785.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            1154..2197
                     /locus_tag="PXD94_001951"
     CDS             1154..2197
                     /locus_tag="PXD94_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL6286786.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEVSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            2437..3543
                     /locus_tag="PXD94_001952"
     CDS             2437..3543
                     /locus_tag="PXD94_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL6286787.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            3518..3886
                     /locus_tag="PXD94_001953"
     CDS             3518..3886
                     /locus_tag="PXD94_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286788.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWSGE"
     gene            3888..4196
                     /locus_tag="PXD94_001954"
     CDS             3888..4196
                     /locus_tag="PXD94_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL6286789.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDNQALIQAVGNLKYTAKVIE
                     "
     gene            4434..4988
                     /gene="srlA"
                     /locus_tag="PXD94_001955"
     CDS             4434..4988
                     /gene="srlA"
                     /locus_tag="PXD94_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL6286790.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            5019..6020
                     /locus_tag="PXD94_001956"
     CDS             5019..6020
                     /locus_tag="PXD94_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL6286791.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPPAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGAVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            6065..6463
                     /gene="srlB"
                     /locus_tag="PXD94_001957"
                     /gene_synonym="gutB"
     CDS             6065..6463
                     /gene="srlB"
                     /locus_tag="PXD94_001957"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL6286792.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLQDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            6511..7299
                     /gene="srlD"
                     /locus_tag="PXD94_001958"
     CDS             6511..7299
                     /gene="srlD"
                     /locus_tag="PXD94_001958"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL6286793.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADLNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYAGEEAAYCTGQSINITGGQVMF"
     gene            7468..>7832
                     /gene="gutM"
                     /locus_tag="PXD94_001959"
     CDS             7468..>7832
                     /gene="gutM"
                     /locus_tag="PXD94_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005737386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL6286794.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYS"
BASE COUNT         2328 a         1480 c         1734 g         2290 t
ORIGIN      
        1 cttctacaca gatagaagag attagaaagc aaatggttaa atttgccacc ctacaactag
       61 ccgataaaga tttagctgag gatgttgtac aggatgcctt aaccaatgcc tataagcatt
      121 cagcctcttt tcggcgagaa gctgcactta aaacttggat ttttgctatt ttaaagaaca
      181 aaattttaga tttactcaga acacgaaaac gacatattcc agtcagttct ctatgtgagg
      241 aagaagaatc accaagtgcc tttttcgatc gccacgatca ttggttggaa catcaagatg
      301 taactgaatg gaggggggta gctcaatcaa cttaccaaaa agaattttgg gcaatttttg
      361 atatttgttt aaataaatta cccgcccagc aagcacgagt atttatgatg cgtgaacatt
      421 tggagatgag tagtgcggaa atttgccaag aatgtgaaat ttctctctct aacttaaatg
      481 tattacttca tcgtgctcgt ttacgtttac aagagtgttt atctcgcaat tggtttagag
      541 aggaatcaaa atgaaatgca ggcaagcaac agaacttatt tcacagtctt gtgatgagaa
      601 acttcgtttg caacaacata tgcgtttgca gttgcatctt ttggtatgcc ctaaatgccg
      661 aaattttaat aaaaatacca agaaattagg caagatatta agagcttact gtaacgataa
      721 agatgctccg ttctgattta ttatacattt taaaaggaga aaaatatgcg ttattggtta
      781 ataagtttaa gcatactatt aagtggctgt gtcgtgccgg ttactcctac ggggcaagcc
      841 ggtatcccta tgccgattat aattggtgtc tcagatccac ttgattccaa agaggttcac
      901 atttgtactc taaagccatt tactcaggaa tatcgtagtg aacatagtag ccgaggcaga
      961 gccaaattaa atgtacaaaa gcaatgccgt gcaaaccaca gtgaaatgtt ttgtgaagaa
     1021 aaagatattg tttgcaaaac ctatgattag ttaaacaagc ggtcgtattt tatctgtttt
     1081 ttgcaaaatt ttcctaaaat ttgaccgctt gtttatttaa gcagataaca tcaaaatttt
     1141 atgaggattg tctatgcaac ccatcaatat tgccatcgca ggtgccggct tttccagccg
     1201 agtttttcat atcccgtttt tgaaacaaga tgctcgtttt aatattgtaa aggtatatga
     1261 acgaacctct aacaatgcgg cagaatggtt gccgaatgca gaaacagtgc gggatttcaa
     1321 cgctctttta acgccagaag tggatttagt gctgattacc acaccgaatc aaacccatta
     1381 tgcaatggta aaagagtcat tgcttgcagg caaacacgtt ttagttgaaa aaccgttagt
     1441 ggcaacagtg gctgaggcgg aagagttggc ggaacttgca aagcggcaag gaaaggtgct
     1501 ttatgtgtac caaaaccgcc gttgggacag ccatattgcg acggcaaaat cgctaatgga
     1561 gcaaaattta ctgggagagt tggtagattg cgaaattcgt tttgaccgtt atgcgaaaga
     1621 gaaaaatgtt aaagtatgga aagaatcagg cgagcttggt acaggtttag tgtatgattt
     1681 aggcgtacat ttaattgacc aagctttgta tcttttcggg aagccggagg cggtgtatgc
     1741 tgatgtgcgt tatcagcacg aaggggcatt ggttgatgat aactttgata ttcagctcta
     1801 ttatgctaac ggttttaaag tggtgctgaa agccagccga tatgctcgtg aaccaagccc
     1861 gacatttgta ttacacggaa aattaggctc ttatctgaaa caaactcccg ataatcaaga
     1921 agatttgctg aaaaatggcg tacaacctat tggcaaagat tggaatatcg aagaaaagga
     1981 gaactgggga attttacaca ctgaagtgga aggtaatgtg gttcgccagc cctatcgaaa
     2041 tgccgaagtc agttatcaaa acttgtttga tgatttgtac caagccatcg ctcacaatgc
     2101 tgagccaatt atcaaactcg aagacgtcat ttttgtgcta aaagtgatcg aaagcgtatt
     2161 tgaaagtgca aaattagggc aaaaaatcta tttgtaaaat tttgaggatt tttgaccgct
     2221 tgttattctc tctgatggcg caagcgtcca cgcttgtgcc tattgattat tttagctttg
     2281 tttctagtac aagcgtggac gcttgcacta tctaaaaaat catctccccc attgcttttc
     2341 ccatctcgat tggctaatat gcaacctcaa tccgtagtta gaatacttaa tatgttaagt
     2401 tttaactgtt ttttcactct aataaggatc atttcaatga ataaacttat tgcctctttg
     2461 gtggcttcaa gctgtgcgac ggcttttgct gctcctgttc aacagaacaa tttccacacc
     2521 actacccaca cttacgaatt tacgcaatct tatgatttag ttgtgccaca aggctcgaaa
     2581 ggaacaacta atttgtggat tccgttgccg tctgataccg attatcaaac ggtaaaatcg
     2641 atagagtttg agggaagtta caaaaacgcc tatatcacag aaaataacgc ttatggtgca
     2701 aaaaccttgt ttgccacttg ggatactgat gcaccaaagc gtgatctaaa agtgaaaatg
     2761 gtgatccaaa cccaagatcg tgagcctatg gctcaagggg cgttggcaaa ttaccaaatg
     2821 ccggagaaaa tcacttattc tgttgatgta ttggagtatt taaaaccaac ggctcacatt
     2881 aaaacagacg gtattgtgaa agaatatgcc gataaaattg tgggtaacga gcaaaatccg
     2941 ttgaaaaaag cggaacttat ccacaagtgg attgttgata atatggaacg tgataactcg
     3001 gtgctaggct gtggcgatgg cgatgtggag aaaattctca ccaccaaagt gctaaaaggt
     3061 aaatgtaccg acatcaactc ggtgtttgta gcacttgctc gtgcctctaa cattcctgct
     3121 cgtgaagtgt ttggtattcg tttagggcaa gcggtaaaaa tggggcaata ttccaaaacc
     3181 gcatttggta gctcaaaaga tggcgtggcg aatgaaagcg gcgggcagca ctgccgtgca
     3241 gagttctatt tagcaggctt cggctgggtg cctgtagatt ctgccgatgt cgctaaaatg
     3301 cgtttagccg agaaaaaaga cgtggatgat gctgatgttc aagcggtctc gaaatactta
     3361 ttcggcaact gggaaatgaa ctgggtaggc ttcaatcacg gacgtgattt cgatttatat
     3421 ccacaaccgg aacttacacc actgaataac tttggctacc cttatgctga ggtaggtggt
     3481 gacccgttaa actcgtttga ggcgaaagaa tttggctatg aattcatctc taaagagtta
     3541 taaccataaa tttatcacaa tgtgcgtgac cgccatagtg tcggcggtga cctccacatt
     3601 ctgttgtatt gggccactgg tttatttagt gtttggcatc tcttcccctt ggttgatgga
     3661 gctaaaccaa ctggagtttc tacaactgcc gatgttgctg atttcgctcg ctgcgtttgg
     3721 ctatggcttc tggttgctga atttttccaa caaaatcatc tgcaccaaat acttctcccg
     3781 cagaaccctg gtcattttgt attgggtagt ttctgtggtg gtgatgttct ttttaactta
     3841 cccttatgta ttgccttaca ttttggaaat gtggagcgga gaataaaatg aaaaaatata
     3901 tcgcaacgat gctgttggct tttggtttgg ttcattcaag ctacgcacaa gaaaaaacag
     3961 ctcaaattga aacgaaacaa gcagagaaaa ccgtcgtcct ccatattccg gaaatgtact
     4021 gccagctctg cgtttattta gtcaataaag aactacgagc agtagaaggt gtgatttcca
     4081 ccaaagcctc aatgaaagag cgaaaagcga ctgtggtggt aaaagaaagt gtggataacc
     4141 aagccctgat acaagcggtc ggaaacctca aatatactgc aaaagtcatt gaataaaacc
     4201 aaagctcaaa cattattgtt tgagcttttt taatttagcc ttcctttttt cccccatcta
     4261 atattcaaac ttcacttttg ttttgattta aaatgaaaaa ataatttatt ttgaaagttg
     4321 tttctcattt tgtgatcgct gtcacagttt ttaaaataaa aataatttac tatttgcaca
     4381 tctcataaca gagatgaaag aaaaatattt tattttgaaa aacggagatc ggtatgattg
     4441 aagcaattac acacggagca gaatggttta tcggcctgtt ccaaaaaggc ggtgaaacct
     4501 tagtcggtat gataaacggt attttgccac tcttaatttc attattggtt gtaatgaatg
     4561 ccctgattaa atttgtaggg caagatcgga ttgaacgact tgctcaacgt agtgccggaa
     4621 accctatttc acgttatttg atcttaccgg tgatcggcac tttcgttttc tgtaacccga
     4681 tgacattaag cttagggcgt ttcttaccgg agcgttacaa accaagctac tacgccgcag
     4741 cctcttatag ttgccactct atgaacggct tattccctca catcaacccg ggtgaattat
     4801 tcgtttattt aggtattgcc agcggtttaa ccaccttagg cttaccactt gggcctttag
     4861 ccatcagtta tttattagtc ggtatgttta ccaatttctt ccgtggctgg ataacagact
     4921 tcaccaccgc attctttgaa aaacgaatgg ggatcacctt agatagagaa gtacatttat
     4981 cccactaacc aagtaactga aatacagagg aatagattat gagcaacaaa gcgatttaca
     5041 ttgaaaaagg caacggtggt tggggtggtc cattaacatt acctgttgtg gaaggcaaaa
     5101 aagtgcttta tatgaccggc ggcactcgcc ctgcgattgt taataaattg gtggaattaa
     5161 ccggctggga agcggttgat ggttttaaag aaggcgaacc gccggcagaa gaaatcggga
     5221 ttgcgattat tgattgcggt ggggtattac gctgtgggct ttacccaaaa cgccgtattc
     5281 caaccattaa cattcactcc accggtaaat ccggcccttt agcagaattt attgtggaag
     5341 atatttatgt ttcagcggtg aaagaaaaca atattcgctt aattgatgcc gatggaagtg
     5401 tgcctgtggc aacagagcaa gcaaaaccaa cttcatcaac gacagcaacg gcaaaagagt
     5461 atgactccag caaaaaaatc accgaacaaa gtgatggttt attagcaaaa atcggtatgg
     5521 gaatgggatc ggtggcggcg ttattcttcc aagccggacg agacactatt gatacggtgc
     5581 ttaaaaccat tttaccgttt atggcatttg tttctgcgtt aatcgggatc atcattgcgt
     5641 caggtttagg tgatgtgatt gcccacggct tgacccctct ggcaaacagc ccaatcggtt
     5701 tagttacact ggcgttgatt tgttccttcc ctctcctttc cccattctta gggccgggag
     5761 ccgtgattgc acaagttatc ggcgtgttag tcggtacgca aatcggttta ggcaatattc
     5821 cgccacattt agcactgccg gcactctttg ctattaatgc tcaagccgct tgtgatttta
     5881 ttcctgtggg tctttcaatg gcggaagcaa aacaagatac cgtacgggtg ggtgtaccgt
     5941 cagtattagt cagccgtttt ttaaccggtg caccgaccgt gctgattgcg tgggcagttt
     6001 ccgcattcat ctaccaataa ttaaccacga tacaagcggt ggaatttgaa aaattttttg
     6061 caaaatgcac cgcttgtcaa aacgagagaa catttatatg acgattattt atcaaacgac
     6121 ctttaccaaa atcgggaatt ttgctcagga atcattaaca gacgatatgt taatcacttt
     6181 taaacaaggc gcaccggcag atttacaaga ttactgcttc attcataatc catctgaatt
     6241 aagtagccct ttagaagtag gcgatatcgc tgaatttgat ggcgtagctt atccgattac
     6301 ggcagttggt agcgttgcat ctgaaaactt atccgccctc ggacacatta ccttccgttt
     6361 tgacggggca aatgatgcgg aatttccggg aagcgttcac gttatcggaa caccgccgca
     6421 aggcttaaca gagaactcaa ccttgattat caagcgtgat taatgacgga ttaaaaataa
     6481 tttcaaattg aaaatttaga ggaatacatt atgaacaatg aaaaaaaagt tgctatcgtt
     6541 gttggcggcg gtcaaacact tggagctttt ttatctgaag gcttggcaga tgccggttat
     6601 cgtgtggtgg ttgctgactt aaacggcgaa aatgcacaaa aagtggcagc cgacattaat
     6661 gccaaacaag gcgaaggaaa tgcgattggg gtgcaagtcg atgcggctga tgaaccaagc
     6721 gttgaagcac tagctaaagc aacagatacc gtattcgggc gtgccgatgt attggtttac
     6781 agtgcaggca cagcgaaagc atcgcctatt tgggatttca acttaaacga ttttgatttg
     6841 tcggtaaaag tgaatttaac cggttatttc ttaaccgcaa aacacttctc ccgcttaatg
     6901 attcgtgacg gtattaaagg gcgtatcatt caaattaatt ctaaatcggg taaagtaggg
     6961 agtaagtata attccggtta tagtgcagcg aaatttggtg gtgttggttt aacacaatcg
     7021 ttagcattag acttggcaga taaaggcatt accgtgcatt cattaatgtt aggcaattta
     7081 ttgaaatcgc cgatgttcca atcgttaatt ccgcaatatg cggcaaaact cggcattccg
     7141 gaaagcgaag tagagcaagt ttatattgat aaagtgcctt taaaacgtgg ctgtgattat
     7201 caagatgtgt tgaatgtctt aaaattctat gccggcgaag aggcagctta ttgcacagga
     7261 cagtctatta atattaccgg tggtcaggta atgttctaag caaacaagcg gtcaaatttg
     7321 tcgatttttt tacaacaatt tgcaaaaaat gactaaaata aaccgcttgt taattcgcca
     7381 caccggttaa tatgccacag gggaaagcgt attgttttga aatcgcaggt acgtttaggc
     7441 tctgtggctt tttattaagg agcaatgatg gattcaatta cacttctgat tatattggca
     7501 atgataatgt ggctgttaca agccgttttg ggttggcttc aaattaaagc ctttaaccaa
     7561 gcctttatgg aaatcagtaa gaaaggcaaa gtaacggttg ggcgtaatag tggtcgtttt
     7621 aatcctaaat cggttattgt gttggcgttt gatgatgaga aaaaagtggt agatagcctt
     7681 tgtatgaaag gattttccgt ctttgccaga ccgcagaaat tagcgtctgt gattggtttg
     7741 tctcttgatc aaattgtgcc ggaaaaaatc tttccaagcg ataaacgttc acaatttgcc
     7801 ttaaaagtgg cgatatcttc cggatatagt tg
//
LOCUS       DANLXQ010000118         7831 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.118, whole genome shotgun sequence.
ACCESSION   DANLXQ010000118 DANLXQ010000000
VERSION     DANLXQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.118"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            109..2676
                     /gene="clpB"
                     /locus_tag="PXD94_001960"
     CDS             109..2676
                     /gene="clpB"
                     /locus_tag="PXD94_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL6286795.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALLKQEGG
                     SIASLFTTLNVDVPTLIRELQQILDRLPKVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLAALDDNGDLGKLFKQFGLNKEKLTQAISQIRGGDTVNNQNAEDTRQALK
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIAGAKYRGEFEERLKAVLNDLAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            2890..4032
                     /locus_tag="PXD94_001961"
     CDS             2890..4032
                     /locus_tag="PXD94_001961"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL6286796.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     GKCRVDGFFLEYDTERAGTFEPLRHIKDQQVVLGLITSKFGDLEDKNVIIERIKEAAQ
                     IVPINQLCLSPQCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(4089..5480)
                     /locus_tag="PXD94_001962"
     CDS             complement(4089..5480)
                     /locus_tag="PXD94_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDL6286797.1"
                     /translation="MENTPLIEPSKNTRNSLIFVADAILFIILLNTLPFTPEANKGLA
                     LLIFIAVLWLTEALHVTVTALLIPILAVALGLVKSKEALVAFADPTIFLFFGGFALAT
                     ALHIQQLDRMIANKIMALAKGKLSIAVIYLFAVTAFLSMWMSNTATAAMMLPLAMGIL
                     SKLDQKQNHNTYLFVLLGIAYSASIGGMGTLVGSPPNAIVASQLNLTFADWLKYGLPI
                     MLILFPLMIATLYIVFKPNFSVSFDRSFEKIELNRSRIITLAIFVFIALGWIFGDKIN
                     PIISAFLGINGKIASFDTILALIAAALICITRVANWQQIQENTEWGVLFLFGGGLTLS
                     AVLGQTGASKIMADGVVSLIEGGHFYLIGLIVAAFIIFLTEVTSNTASAALLVPIFIS
                     IAQALNMPPVGLALIIGLGASCAFMLPVATPPNAIVFGTGQIKQSEMIKVGFWLNIIS
                     IFVIATVGYLFWL"
     gene            complement(5640..6539)
                     /gene="cdd"
                     /locus_tag="PXD94_001963"
     CDS             complement(5640..6539)
                     /gene="cdd"
                     /locus_tag="PXD94_001963"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL6286798.1"
                     /translation="MFRTDITERFEQLKAAKNCEVTQAIIEQLIKQDFHGQLSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
     gene            complement(6553..7455)
                     /locus_tag="PXD94_001964"
     CDS             complement(6553..7455)
                     /locus_tag="PXD94_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6286799.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGIGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAMFSIILLNES
                     VHTYHIISAILITAGILICSYKKH"
     gene            complement(7452..7808)
                     /gene="folB"
                     /locus_tag="PXD94_001965"
     CDS             complement(7452..7808)
                     /gene="folB"
                     /locus_tag="PXD94_001965"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL6286800.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
BASE COUNT         2573 a         1643 c         1572 g         2043 t
ORIGIN      
        1 ctttttcaca tttttacttg aaaattgttt accaagctcc atttagtgtt tcaacaagag
       61 atgtaaataa aaatttacac tttctaaact cattgtaagg agaaagaaat gaattttgaa
      121 aaaatgacca caaaactgca agaagccttg gctgaatcac agtcgcttgc ggtaggaaaa
      181 gataatccgt acatcgaacc ggcacatttg ctctatgcgt tgttaaaaca agaaggtggc
      241 tcgattgctt ctttgtttac cacattaaat gttgatgtac cgactttaat tcgtgaatta
      301 caacaaattt tagaccgctt gccgaaagtt caaggcggta acacccaagt ttcacaacag
      361 ttggtcagac tgttaaatca atcagacaaa ctggcacaac aattcggcga cagctttatt
      421 tcatcagaac tatttgttct agctgcactt gatgacaatg gcgatttggg gaaactattt
      481 aaacaatttg gcttaaacaa ggaaaaacta acacaagcca tcagccaaat tcgaggagga
      541 gacacagtga ataatcaaaa tgcggaagat acccgccaag cactcaaaaa atatacgatt
      601 gatttaaccg aaagagccaa agccggtaaa ttagacccgg ttatcgggcg tgatgaagaa
      661 attcgccgtg cggtgcaagt cttacaacgc cgtaccaaaa acaaccctgt gttaattggt
      721 gaaccgggtg tggggaaaac ggcgattgta gaaggcttgg cacagcgtat tgtaaacggc
      781 gaagtgccgg aaggcttgaa aaataagcgt gtactttcgc tggatatggg agcattaatt
      841 gccggtgcga aatatcgtgg tgaatttgaa gagcgtttaa aagcggtttt aaacgacctg
      901 gcgaaagaag aagggcaagt tatcctcttt attgatgaaa ttcacacgat ggttggagca
      961 ggcaaaacag acggagcaat ggacgccggc aacttattaa aaccaagcct tgcccgtggt
     1021 gaattgcact gtgtgggggc tacaaccctt gatgaatatc gccaatacat tgaaaaagac
     1081 gcagcccttg aacgccgttt ccaaaaagta ttggtggacg aaccaacggt tgaagatacg
     1141 attgcgattt tacgtggttt gaaagagcgt tatgaaatcc atcatcacgt tcaaattaca
     1201 gaccctgcca tcgtggcggc ggcaacgctt tcgcaccgtt atgtgtctga ccgtcaattg
     1261 ccggataaag cgattgactt gattgatgaa gccgcttcaa gcctgcgtat ggagattgat
     1321 tctaaacctg agccgttgga taaattagaa cgccgcatta tccaactcaa attggaacgc
     1381 caagcgttac aaaaagagga agatgaagcg agccgtcagc gtttagccaa attagacgaa
     1441 gaaatggcag cgaaagagcg tgaatattcc gagctggaag aaatctggaa agcggaaaaa
     1501 tccgccttac ttggtacaca gcatattaaa tcggagttgg aaaatgcccg aattgcgatg
     1561 gatcaggctc gccgtgaaaa cgatttcgag aagatgtcgg aattgcaata tggcgtgatt
     1621 ccaaacttgg aaaaacagtt acaagcggtc gaaaatggcg aaaaaaatgc agatgctcag
     1681 ctcctccgta ctaaagtcac cgaagaggaa attgcggaag tgctttcaaa agcaacgggc
     1741 attcctgttg caaaaatgat ggaaggcgag aaggaaaaac tccttcgtat ggaagaagta
     1801 ttacacgctc gggttatcgg gcaaaatgaa gccgttgatg cggtggcaaa tgcgattcgc
     1861 cgtagccgtg ccggactttc cgatccgaat aaaccgattg gttcattcct attcttagga
     1921 ccgaccggcg tgggtaaaac cgagttaagc aaaaccttag ctaatttctt gtttgacgat
     1981 cctgatgcta tggtgcgaat tgatatgtct gaatttatgg aaaaacacag cgtatcccgc
     2041 ttagtgggcg cacctccggg ctatgtcggc tacgaagaag gcggctattt gaccgaagcg
     2101 gttcgccgcc atccatattc ggtagtgtta cttgatgaag tggaaaaagc tcaccctgat
     2161 gtgtttaaca ttttattgca agtgttagat gacgggcgtt taaccgacgg gcaaggcaga
     2221 acagtcgatt tccgcaatac ggtagtgatt atgacctcaa acttaggctc gcatttaatt
     2281 cgagaaaatg cgtataaagg ctatgatgtg gttaaagaaa tggtgatgga agtcgtcgga
     2341 cagcacttcc gccctgaatt tatcaaccgt attgatgaaa cggtgatgtt ccactcactt
     2401 ggtcaagagc atatccgttc gattgcgaag attcagctac aacgcttaat ccaacgcttg
     2461 gcagaacgag gctatgaggt gacagtaacc gatgcagcat tagatcatat cggcaaggct
     2521 ggtttcgatc cgctgttcgg agcaagacca ctgaaacgtg cgattcaaca agagcttgaa
     2581 aatccgcttg ctcaacagat cttaagcggt aaattattac cgaataaagc cgttacggtg
     2641 gattaccaaa acgggcagat tgttgcaaca caataatgtc tccgatggtg cgagcgtcct
     2701 cgctcgtacc ttacaagcgg tggatttatc gtaaaaattt gcaaaaaatt gctcgaaaat
     2761 aaccgcttgc acagtaaaaa ttttcatatt gcccttcatt aattttcata agctaatgaa
     2821 gggcttttta ttactctttt tgctatcatt aacggcgttt ataaccaata caattacaag
     2881 gagtaattta tgagccaatt atttccggaa gcaacaatcc gcactcaagc cccgtatcgt
     2941 aatgatattg taggcagctt tctacgtacc gatgcgttaa aaaaagccag agaagatttc
     3001 aatcagcaaa aaatcacccg tgacgaattg cgtgaagtca cacgccaaga ggtcaaaaaa
     3061 ttagttgagt tgcaaaagca acacggtgta aaagcagtct ctgacggtga atttagccgg
     3121 acttggtggc atttagattt cttagctgaa cttgatggaa tggattggca agaaaccgaa
     3181 gtctttaacg tgaactttac cggtcataaa cccaaagggc aaacggtaaa aatcgtgggc
     3241 gatattgatt tctccgaaag ccaccctttt gtggacgctt atcgtctttt aaaagaagct
     3301 gccggcgatt acccaaccaa attcacgatt ccggcaccga caatgttaca tattatctgc
     3361 tgtgtgcgtg cggaagatta ccaaccgtta ccacaatatc aagacgaaca aaaactgtac
     3421 gatgatattg ctaatgctta tatcaaagca atgcacaaat tctacgatat ggggctacgt
     3481 aacttacaat tagacgacac cagttgggga caattctgtg ccgaagataa acggaaagag
     3541 tttgcagcca gaggatttaa tttaactgat ttagcggaaa aatatgtcgc attattaaac
     3601 cgtattgttg atgcaaaacc ggcggatatg tcaattacga tgcacatctg ccgtggtaac
     3661 ttccgttctt cttggttctc ggaaggcggt tatggaccgg ttgccgaagt attattcggt
     3721 aaatgccgtg ttgacggctt cttcttagaa tacgataccg aacgtgcagg cactttcgag
     3781 ccactacgcc acattaaaga tcaacaagtg gtactaggct taattacctc taaatttggc
     3841 gatttagaag ataaaaatgt gatcattgag cgtattaaag aagcggctca aattgtgcca
     3901 atcaatcagc tatgcttatc accacaatgc ggctttgcct ccaccgaaga aggcaatatt
     3961 ttaaccgaag ccgcacaatg ggcgaaacta aacttaatta aagaaattgc ggaagaagtt
     4021 tggggtagct aatcctctat aatacaaata gccgagtaaa tactcggcta ttttagtttg
     4081 ttttaacatt acaaccagaa gagataccca acagtcgcaa tcacaaagat agagataata
     4141 tttagccaga aaccaacttt aatcatctca ctttgtttaa tttgccctgt accaaataca
     4201 atcgcattcg gtggggtggc aaccggcaac ataaaggcgc aagatgctcc tagaccaata
     4261 attaaggcta atccgaccgg tggcatattt aatgcttgtg ctatagagat gaaaatcggc
     4321 actaacaaag ccgcactggc tgtgttagag gtaacctcag ttaggaaaat aataaatgct
     4381 gccacaatta aaccaattag gtaaaaatgc cccccttcaa ttagtgagac gaccccatca
     4441 gccataattt tgctcgcacc agtttgtcct aatacagcac ttaacgtcaa accaccacca
     4501 aataggaata atacccccca ctcggtgttt tcttgaatct gttgccaatt tgcgacacga
     4561 gtaatgcaaa taagtgctgc ggcaatcaat gcaagaatgg tgtcaaagct cgcaattttg
     4621 ccattaattc ccagaaatgc agaaataata gggttaattt tatcaccaaa aatccaacca
     4681 agcgcaataa agacgaaaat agccaaggtg ataatacgag aacgatttaa ttcaattttc
     4741 tcaaatgatc gatcaaaact aactgaaaaa tttggtttaa atacgatata tagtgttgca
     4801 atcattaatg ggaataaaat tagcataatt ggcaaaccat attttaacca atcagcaaaa
     4861 gtcagattca attgagaagc aacaatcgca ttcggtgggc ttcccactaa agtacccatt
     4921 ccaccaatac tggcactata agcaattcct aataacacga aaagataagt attatggttt
     4981 tgtttctgat caagtttact taaaatcccc attgccaaag gtaacatcat tgcagctgta
     5041 gctgtattac tcatccacat tgacaaaaac gctgttaccg caaataaata aataactgca
     5101 atcgataatt tacctttagc taatgccata attttattgg caatcatacg atctagctgc
     5161 tgaatgtgta atgctgtcgc taaggcaaag cctccaaaaa ataagaaaat cgttggatca
     5221 gcaaaagcaa caagagcttc ttttgattta accagcccca gtgctacagc caaaatcgga
     5281 attaataatg ccgtaaccgt aacgtgtaat gcttctgtta gccataagac ggcaatgaaa
     5341 attaacaatg ccaacccctt attggcttca ggggtaaaag gcaaggtatt taacaaaata
     5401 ataaataaaa tagcatctgc aacaaaaatc aagctattgc gtgtattttt actcggttca
     5461 ataagaggag tattttccat aaaaacctca caaaatatag ataatcagtg gtatcactat
     5521 atataaaaaa acgatatagg caaatataat tgttgcaaaa gtgtgaagct catcaaaaaa
     5581 tttaacacta aaaaataaat tgcttaaaga tacagcacct ttgcaaaaat aaatcaaaat
     5641 tacaccgctt gtagcgaaag gtattcaatc tctaacccaa atagtgattt tgccaatgct
     5701 tgagtcattg cataactact gagagagcct ttcgcctcag ccagcacaac tcgagtaacg
     5761 ggaatatcgg caagccctaa aaataatcga tagttcaacg cgctttgcaa cggtaaaaaa
     5821 gtaggattaa aagctgcatt ctcagtatat ttaccacaaa caatacgctc acctacttgc
     5881 agtgcaaccc cactaaatgc gttgctgtaa ggggcataag cattattggc agcaaaaatt
     5941 gccgcttgtt gtaaatgatc gcccttaacc tcaaattgtt gcggttgttc atcaaataag
     6001 actttctcaa tccctaaatc tttcggtcca aaactatccg gcaaatagct atggagcaaa
     6061 ttattttggc tatgcggtaa atgtactttt aagcttgccg cactgtttaa ttcattcata
     6121 aattggcggc aatgcccaca aggggtatag ttcacaacca catctgttat caaagtttcc
     6181 cctgctaaaa atgcgtgcga gattgcactt tgctctgcgt gaacggattg ttgcatacat
     6241 tccccggaaa actcttggtt tgccccaaaa tagaaatctc cgtttttgcc aatcgcaacc
     6301 gcccctacat taaaatcgga aactggtgta accgcataac aggcagcaat ggcaatgcaa
     6361 tataaagcca actcaacacg agagcgttta aatttttcac agagttcatc aaccacttcg
     6421 taagagagct gcccgtgaaa atcttgcttg ataagttgct caataatagc ttgtgtaact
     6481 tcacagtttt tcgctgcttt taattgttca aaacgctctg taatgtctgt tcgaaacata
     6541 ctgtacctcc gtttaatgtt ttttatagct gcaaatcagt atccctgccg taatcagtat
     6601 ggcactaata atatggtaag tatgaactga ttcatttaat aaaataatac tgaacattgc
     6661 tgcaaagagc ggcgtaaaat tagtcattac cgctccttta gcagaaccga ctattgcaat
     6721 gccgaaattc caaaacgcat aagagagaat agaagggccg ataaccaaat agcctatccc
     6781 actccattgt aaagcggtca aatttgctaa attttttgca gattctgcta catattcata
     6841 gataaaaaat ggcgttaacg ttagaactgc cagccccact aatacggtca aaaacaccgt
     6901 attgcttata ccttgtggac gtagccgaat cacacaacaa taaaccgccc agcttatagt
     6961 agaagcaatc gcccataaat caccttgatt aattgccaag ctgaataagc gagttagctt
     7021 cccttgcatc aatagccaca gcactcccag caaactgatt attgccccac ttagaataat
     7081 cgggctgatt ttctccttaa aaacgagccg atttaaaaat agcaccaata caggcgaaat
     7141 agctaaatag agactcgcat tcaatgctgt tgtcgttttt aaaccttcat ataaggtagc
     7201 cgggaataaa attaccccaa gcagacctaa tacccacatc acacctaaac gaggttttaa
     7261 taagtgcatt acccgccaag ttgggcgaaa aaagagcaaa actaaaatta aaaaagccgg
     7321 caaccaacgc aaataggtta aggtaatagg ctgaactgcc ccacttaaca ctttgcctaa
     7381 tacaaaattc ccaccccaaa aaagagtagc aacgattaaa ctgaaatacc ccaatttcag
     7441 tgatgatgag atcattgatg acctcgttct acaatcacac ctacactgct tgcttgggca
     7501 accgctttcg gcttatgcag ttcaatccga atccaagaaa gttgatactc tttttgtaat
     7561 agttccgcca gttgataggc aacagtttca actaatttga aaggttgaga ttcaacaaaa
     7621 tccagcactt tttgcgatac ttccgcataa ttcaagcaat attgtacatc atcggtttct
     7681 gccgctttgg aaaaatccca cgccatatca atgttaaaaa ccaagcgttg cttaatcgta
     7741 tgctcccaat tgtaagcccc aatggaagca aaagcggtta gttctcgaat aaaaacctta
     7801 tcactcattt ttcgtcctgt tttattttct g
//
LOCUS       DANLXQ010000119         7812 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.119, whole genome shotgun sequence.
ACCESSION   DANLXQ010000119 DANLXQ010000000
VERSION     DANLXQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.119"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..2592
                     /gene="topA"
                     /locus_tag="PXD94_001966"
     CDS             <1..2592
                     /gene="topA"
                     /locus_tag="PXD94_001966"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL6286801.1"
                     /translation="VIVESPAKAKKINQYLGSNYVVKSSVGHIRDLPKSGSDEKTEKA
                     KPISTKGLTAAEKAKIKAEKERVALVKRMGIDPYNGWKANYQILPDKEKVVSELKSLA
                     KKADHIYLATDLDREGEAIAWHLREIIGGDDSRFSRVVFNEITKDAIKKAFDDPQPLN
                     IDRVNSQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQS
                     EEFWTILANTQINRKKLPLELTTYKGKKFSASNEAEANVAVRALEQSDFIVSNIEAKE
                     TSSRAAAPFITSSLQRAASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSREALA
                     MARGYIQDNFGANYLPEKANVYRSKDNAQEAHEAIRPSDVNVKMADLKGMDKDAERLY
                     DLIWRRFLACQMLPARYDSTAVTVSAGDYELKANGRVLRFDGWTKVLPVQGKNPEDQE
                     LPAVELHQKLTLDEIVPSQHFTKPPARFNEASLVAELEERKIGRPSTYASIISTIQER
                     GYVRTENKRFYVEKIGEVVTDRLNESFKDLMNYDFTANMEEVLDQIAEGSKNWKEELN
                     TFFKHFSEELSQAELDELEGGMKPNSLVPTDIDCPTCGRKMAIRTASTGVFLGCTGYA
                     LPPKERCKTTMNLIPEAEFFNVLDSDSETKALMARKRCPKCDSAMDSYVIDAERKLHV
                     CGNNPNCDGHIIEQGSFKIKGYDGPVVECDKCGSDMHLKLGRFGKYMGCTSCDNTRKI
                     LKNGEVAPPKEDPIAFPELKCEKADAYFVLRDGASGVFMSAHNFPKVRESRAPKVVEL
                     AQYRERLPEKLQYLADAPQQDPEGNEAIIRFSRKEKRQYVTSEKNGKATKWIVDYLDG
                     KWVERKK"
     gene            complement(2642..3580)
                     /gene="yvcK"
                     /locus_tag="PXD94_001967"
     CDS             complement(2642..3580)
                     /gene="yvcK"
                     /locus_tag="PXD94_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL6286802.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(3594..4670)
                     /locus_tag="PXD94_001968"
     CDS             complement(3594..4670)
                     /locus_tag="PXD94_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL6286803.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(4701..5456)
                     /locus_tag="PXD94_001969"
     CDS             complement(4701..5456)
                     /locus_tag="PXD94_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6286804.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
     gene            5608..5817
                     /locus_tag="PXD94_001970"
     CDS             5608..5817
                     /locus_tag="PXD94_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL6286805.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(5866..6588)
                     /gene="bioD"
                     /locus_tag="PXD94_001971"
     CDS             complement(5866..6588)
                     /gene="bioD"
                     /locus_tag="PXD94_001971"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL6286806.1"
                     /translation="MPSLFITGTDTNVGKTVVTRAIIQTLTKHSFPVIGYKPIACGGD
                     DSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDINSYTFIHSSTPVFAALDAVHHI
                     QLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYYFADWVMQNNMPVVLVVGIKEGC
                     VNHALLTANEILQRGVKLVSWVANRVNPGLRHYHELIELLTQKINAPLLGQIPYMGHP
                     EHKELSSYIQNPEPLLAYFSKP"
     gene            complement(6636..>7812)
                     /locus_tag="PXD94_001972"
     CDS             complement(6636..>7812)
                     /locus_tag="PXD94_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL6286807.1"
                     /translation="NKALHLGKIYHLIEQFELISRTDLAKLSGLAPASVTNLTKILID
                     NHFILERTVQTTLSRGRPSVGLAVSNFFWQLLCVTVSSHKIEISLCKLNGAEIYKQDY
                     TISAENYPRLNDFILNFITNFRQLYCVNTSQLLAVSISVIGKINAEKDSITQLGNKNI
                     FCNITSKLSPLFHCPILLSEHFQLWLLAESTVGSLINQDNAIFLQLDDTVNLSVLLSG
                     SLLSKQSKMNVDNMLMPRFSHLSDEIFPELDEIHRYKLVNQVTFPAIVRLIDRYLPNN
                     LKSQEQKISWLCQKIDENQPLALAILSHITDNLAYALMNLVNIFSSEKIMLNSPLIKI
                     KEPLFTQISEKLHKNLLQTNLKIDLVTSQYDWNSPLIACSAIKQGIYDGNLIKDRIN"
BASE COUNT         2495 a         1415 c         1613 g         2289 t
ORIGIN      
        1 gttattgtgg agtctccggc taaggcaaaa aaaattaacc aatatcttgg tagtaattat
       61 gtggtgaaat cgagtgtggg gcatattcgt gatttaccga aaagtggtag tgatgaaaaa
      121 acggaaaaag cgaaaccgat ttcaaccaaa gggctgaccg ctgccgaaaa agctaaaatc
      181 aaagcagaaa aagagcgtgt tgcattggtt aaacgtatgg gtatcgaccc ctataatggc
      241 tggaaagcta actatcagat tttgccggat aaagagaaag tggtttccga gctaaaatcg
      301 ttggcaaaga aagcagatca tatctatctg gcaaccgact tggatagaga aggggaggcg
      361 attgcgtggc atttgcgtga aatcattggt ggtgatgaca gccgttttag ccgagtggta
      421 tttaatgaaa tcaccaaaga tgcgatcaaa aaggcttttg acgatccgca accgctcaat
      481 attgaccgtg taaattccca gcaaacccgc cgctttttag accgtgtagt gggctttatg
      541 gtgtcgccat tattatggaa aaaagtagct cgtggtttat cggcagggcg tgtgcaatcg
      601 gttgccgtaa aattactggt tgagcgtgag cgtgaaatca aggcgttcca aagcgaggag
      661 ttttggacta ttcttgccaa tacgcaaatt aatcgtaaaa aattaccgct tgaattaact
      721 acttataaag gtaaaaaatt ctcggcaagc aatgaggctg aagcaaatgt tgcagtgcgt
      781 gctttggagc agagcgattt tattgtttct aatattgaag ccaaagaaac cagctctcga
      841 gctgctgcac catttattac ctcatcgtta caacgtgccg ctagcactcg tttaggtttt
      901 ggggttaaaa agacgatgat gttagcccaa cggttatatg aagcgggtta catcacctat
      961 atgcgtactg attcaaccaa ccttagccgt gaagcattag caatggctcg aggttatatt
     1021 caggataatt tcggggcgaa ttatttgccg gaaaaagcaa atgtgtatcg cagtaaagat
     1081 aatgcccaag aagctcacga agccattcgc ccgtcggatg tgaatgtcaa aatggcggat
     1141 ttaaaaggaa tggataaaga tgccgagcgt ttatacgatt taatttggcg tcgtttcttg
     1201 gcgtgtcaaa tgttgcctgc tcgttatgat tctaccgcag tcacagtgtc tgccggagac
     1261 tatgaactca aagcaaacgg gcgtgtactg cgttttgacg gctggacgaa agtactgccg
     1321 gtgcaaggca aaaatccgga agatcaggaa ttaccggcgg ttgaactgca tcaaaaatta
     1381 acgcttgatg aaattgtgcc aagtcagcat tttactaaac cgcctgcacg ttttaatgag
     1441 gcgtctttgg ttgctgagtt agaagagcga aaaattggcc gcccttcaac ctatgcttca
     1501 attatttcga cgattcaaga acgtggctat gttcgcaccg aaaacaagcg tttttatgtg
     1561 gaaaaaatcg gtgaagtagt gacggatcgc ctcaacgaat cattcaaaga tttaatgaat
     1621 tatgatttca ctgccaatat ggaagaggtg ctcgaccaaa ttgcagaggg gtctaaaaat
     1681 tggaaagaag agttgaatac tttctttaaa catttctctg aagagttgag ccaagcagaa
     1741 ttagatgaac ttgaaggcgg aatgaaacca aacagccttg tgccaaccga tattgattgc
     1801 ccgacttgtg gacgaaaaat ggcaattcgc accgcttcca caggggtatt tttaggttgc
     1861 accggctatg ctttaccgcc aaaagagcgt tgcaaaacga caatgaattt aattccagag
     1921 gcggaatttt tcaatgtgtt agattcggat tctgaaacca aagccctaat ggctcgcaag
     1981 cgttgcccga aatgcgattc ggcaatggat agttatgtga ttgatgctga acgtaaatta
     2041 cacgtttgtg gtaataatcc aaattgtgat ggtcatatta ttgagcaagg ttccttcaaa
     2101 attaaaggct atgacggacc tgtagtggaa tgcgataaat gcggttcaga tatgcactta
     2161 aaactgggtc gtttcggcaa atatatgggc tgcacaagct gcgataacac ccgtaaaatt
     2221 ctgaaaaacg gggaagttgc tccgccaaaa gaagatccta ttgcttttcc tgaattaaaa
     2281 tgtgaaaaag ccgatgccta ttttgtatta agagacggag caagtggggt gtttatgtca
     2341 gctcataact tcccgaaagt gcgtgaaagc cgtgcaccaa aagtggtaga gttagctcaa
     2401 tatcgtgaac gtttgccgga gaaattgcag tatcttgccg atgctccgca gcaagatccg
     2461 gaaggaaacg aggcaattat ccgttttagt cgtaaagaga aacgccaata tgtgacttct
     2521 gagaaaaacg gcaaagccac caaatggatt gttgattatc tagacggtaa atgggttgag
     2581 cgtaagaaat aaagcttaaa agacaagcgg taaaattttc taaaaatttt accgcttgct
     2641 tttagatgtt ctgagcgagt agttcatcaa ttgctttact taatttattg cgatcgtgtc
     2701 ggtaataaac gtcattcgca gataagtttt ttcgcaagat ttgaatatgt tcgggaagtt
     2761 cagaaccggc atcatcagtc gtaattaccg cattaattct agcctgtccg atacagttct
     2821 ccatccattc aattcgctga ttaagtgata gttgcccagc tgggccatgt tcttgtccga
     2881 tattgtcaat aaatactact cgggctttgc tttgagcaat agagtttgct atgcctgcca
     2941 ccagcaaatt cggcataata ctggtaaaaa agctacctgg gcctagcagt attaactcag
     3001 cactttgaat agctgaaatg gcttctggtg tagcaacaac catagtatct aacgctaaat
     3061 gttgtggtgt ttctgctaaa gaatcaattt gcacttcacc aaatactgat tcgcctgaat
     3121 gcaactgtgc aattaaatgc accggtattt cagacattgg gataagttgc gatcttacgt
     3181 gcaataattc tcgcaccaaa ttaatccctt ctagcggtcg gatttgcata ttttcaaggg
     3241 cggtgagaat taaattgcct agattatgtc cagcgagttc gcctgtgcca gcaaaacgat
     3301 actcaaacaa ccgagaagct atcgttggtt taacgataat ttgatttaaa cagttgcgta
     3361 aatctcccca agcaatcacc ccttgttgtg aacgtattct accggttgag cctccattat
     3421 cggtagtggc aacaatgccg gtaagccgtt cctttaaaaa agagagcgcg gagagcaatc
     3481 gacctaagcc gtggccccca cctagtgcaa caactgcatt gagttggttt aaatagtgat
     3541 gtcgggtagg tttaaaagga ataatatctg tattttccat cttgggattt cgtttatttt
     3601 tctaaaataa tggcatctga ctgggctgaa ggcaatgctt gtaatacagc tttaaccaaa
     3661 gtcgctaaag gaatcgcaaa aaacactccc caaaagcccc ataaaccacc aaagatgagt
     3721 acggcaataa taattgtcag agggtgtaga tttactgctt cagaaaataa aaagggtacg
     3781 agtaaatttc cgtctagcaa ctgactaata ataaatgcga gcattaaata gtagaaatcc
     3841 ggcgataacc cgaactggaa tagagcaatt aacattaccg gaatgctgac taaaaccgca
     3901 ccgatgtagg gaactaacac tgaaatccct accgctacag aaagtagtaa cggatagcgt
     3961 aaatcaaaaa agaggaaaat aatataagtg acaaccccaa caattagaat ttctaaaaat
     4021 ttacctcgaa tatagttggc aatttgttgt tgcatttcaa accatactcg tgttgcaagt
     4081 cgacggtttt gcggtaatac tttactaaaa gaacgcatta aaacgatttt atctttcagt
     4141 aagaaaaaga ccatgagtgg gactaagaag gtgtaaatgc ctaagccaac taagctgatg
     4201 atagagttaa tcgagagtgt tagaagtgat tctcccatac tcaaaatatt acttttagcg
     4261 gtattcacaa tggaatctag ggtcgcataa tctactaatt cagggtaatg ctccggtagg
     4321 gcttgtaacc aagcattgag cagattaaac atcgaaggta aatcttgaat aaaggtaact
     4381 gcttgattcc aaaggctggg tagtagtact acgcctaaaa aagataataa agcgatgaaa
     4441 cctcctagta ctagaatgac actcaaagtt cgaggtaatt tgagtttgct cgacaaaaaa
     4501 cggattggcc attccaataa ataggcaaac acaagtgcaa ccagaagggg cataagcaga
     4561 tcgctaaaaa aatagataat accgaaaccg atcaatagaa tactaaacag cacaactatt
     4621 tgcgggtcgg taaatttttt tcgataccaa tcttgcaaca tttgaaacat atggattgcc
     4681 tttaaattat gcgatgaaaa ttatttctgg ctacagactg caatgaaccc tagctttttg
     4741 tccgggtcat taaatagttt gtacatttta ttaaaggttt cgcgattttc tggttttaat
     4801 gcattgccga taattttcat ggcgcctaaa acaccttcat catgaatcag acctttaggg
     4861 gaaagtagag acatttctcc ggaaaaggta tcaacattac ggaaaccaca acgttggaat
     4921 aatgatttcc agtctgcttt agcaagtgga gagacagtga tattgattgc atcacgcaac
     4981 tcggctaata aagtatcgga gtctttatct tggataacaa tatcgtgcgt taataaaaag
     5041 ccatttggtt ttaatacacg aaaatattca cgtattgcct tttctttcgc ttctagagga
     5101 agcattgtca gcatggcttc attaattaca atatcaaaac tttcgtcgtc aaatggcagt
     5161 tttgttgcgt tggcgcgctg tacttgaatt aaatgctcaa catggttttc tttgatattt
     5221 ttacgtgctt tttcaagcgc atcttcgtct aaatcaatac caacaatttg gcattcaaat
     5281 tgttgagcta attggattgc agtagtcccc atgttacagg caacttcgag cactttttta
     5341 tttttatcaa aatccccgtt tgcaattaac caatcagttg ctaatttccc cccggggcgt
     5401 aaacgagttt tgcctaaacg agctaaaaaa tgatgaccaa cttcttcttt tttcataaaa
     5461 acctctggat ttgcaaaata cgttaaatat atgaccgctt gtattgtgat ctatgttcgt
     5521 tattatactc aattttttca ttttcttata tacaacatca acagattttg ccagaataca
     5581 acctcatttt gatatgagga aaatagaatg agtaagcaaa agaatgatta cctccaccag
     5641 cgaggtcaaa ttaaagaaag tgcagtaaaa gcattagtga ctgatccact ttttagacaa
     5701 cgtgtagaaa gaaatcgtaa agggaaaggg agctatcgca gacaagaaaa acacaaaaaa
     5761 actatctatg ggcaaaaaga aaccccattt aaaaatattc ggttagagtc tttttaaatg
     5821 gggttttatt tgtagcttat tgtagtttta taagcctgat aaacactatg gtttactgaa
     5881 ataggccaac aacggttctg gattttgaat ataagaagaa agttctttat gctctgggtg
     5941 acccatatag ggaatttgcc caagtaaagg cgcattaatt ttttgtgtca gtagttcaat
     6001 taattcgtga taatgccgta gccctggatt tactctgttt gcgacccaac ttactaattt
     6061 tacaccacgc tgcaaaattt cattcgcagt aagtagtgca tggttaacac aaccttcctt
     6121 aattcccacc actaaaacaa ccggcatatt gttttgcatt acccaatcag caaaatagta
     6181 atccttgtta ataggagtta gccagccata ggttccttct acgacaacat ttggataggc
     6241 attttctagt cgttttaaat ctgtgtttaa tttttccagt tgaatatgat gaactgcatc
     6301 taaagctgca aatacagggg tactggaatg aataaaagta tagctattaa tatcacgata
     6361 gctaacatta gttggacagc tatctagtat ggttaatacg tctgaattat cttcagtagc
     6421 ataatcatat tgagtaggtt ctgtgggtaa tgaatcatca ccgccacaag caatgggttt
     6481 atagccaatt acaggaaaac tatgttttgt aagtgtctgt atgatggctc gggtaaccac
     6541 agttttccct acgttggtgt ctgttccggt aataaataat gagggcataa atggctcctt
     6601 tcgaaaatat ggctggtagt ttaaccaaaa atagatcaat ttattctgtc tttgatcaaa
     6661 tttccgtcat aaattccttg ttttattgct gaacaggcaa ttaatggact attccaatcg
     6721 tattggctgg taactaaatc gatttttagg tttgtttgta acaaattttt atgcaatttt
     6781 tctgaaattt gcgtaaataa cggctctttt attttgatca atggcgagtt tagcataatt
     6841 ttttcactag aaaatatatt aactaaattc attaatgcat aagctaaatt atcggtaata
     6901 tgcgataaaa tagctaatgc aagcggttga ttttcatcaa ttttttgaca aagccagctg
     6961 attttctgtt cctgactttt taaattatta ggcaaataac gatcaatgag tctcacaata
     7021 gctgggaacg tcacctgatt gactaattta tagcgatgaa tttcatctag ttcagggaat
     7081 atttcatcac tgagatggct aaatctaggc ataagcatat tatcaacatt catctttgat
     7141 tgtttagaca gtaaactacc actaagcaat acacttagat tcacagtatc atctaattgt
     7201 agaaaaatag cattatcttg gttaattaat gagccaaccg tagattctgc aagcaaccac
     7261 agctgaaaat gttcacttaa taaaatagga caatgaaaca gcggcgagag tttagatgta
     7321 atgttacaga atatattttt gtttcccaat tgagtaatac tgtctttttc tgcattaatt
     7381 ttaccgatca cactaataga tacagccagg agctgactag tattaacaca atatagttgg
     7441 cggaaatttg taataaagtt aagaataaaa tcatttagcc taggataatt ttctgctgag
     7501 attgtataat cttgcttata aatttctgca ccattgagtt tacaaagtga aatttcaatt
     7561 ttatgagatg aaacagtaac acaaagtagt tgccaaaaaa aattagaaac agctaatcca
     7621 acggaggggc gacctctgga taaagtagtc tgaaccgtac gttctaaaat gaagtggtta
     7681 tctattagta ttttagtcag atttgttact gaagctgggg ctaaccccga tagtttagct
     7741 aaatctgttc ttgaaatgag ttcaaattgc tcaattaaat ggtatatttt acccaaatga
     7801 agggctttgt tg
//
LOCUS       DANLXQ010000120         7572 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.120, whole genome shotgun sequence.
ACCESSION   DANLXQ010000120 DANLXQ010000000
VERSION     DANLXQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.120"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            6..737
                     /locus_tag="PXD94_001973"
     CDS             6..737
                     /locus_tag="PXD94_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL6286808.1"
                     /translation="MKLITAMILPPFNIILLWLLALILFSLGYKYAAYFFALLGILIL
                     YIFSIPYTSQKLGDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITGIPLE
                     RMRYAAYLHKETGLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTTKENA
                     IFSREILEKENIHKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKLNFMH
                     FIPQAGAMNSNMLALKEWIGYWKEK"
     gene            complement(814..3012)
                     /gene="priA"
                     /locus_tag="PXD94_001974"
     CDS             complement(814..3012)
                     /gene="priA"
                     /locus_tag="PXD94_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL6286809.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGALPIKLRNGD
                     STERVQPNYFVVTEQGRKSLIIGENKKAKKQVERLTELAECAKYFEKPTACSPSAWKG
                     LLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTTFASFL
                     LNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEIDVLHSN
                     MNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDGWRYHA
                     RDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQIIDLK
                     TQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEACNKPFT
                     YHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQVTRID
                     RDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALFSTDFR
                     AEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEALKMRQL
                     MSLPPFSSQVLFKATGKDNQQTLQLLEQLADYFSQKIQQQNGQGFQLPPPFSAPMAKK
                     AGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
     gene            complement(3024..3636)
                     /locus_tag="PXD94_001975"
                     /pseudo
     CDS             complement(3024..3636)
                     /locus_tag="PXD94_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(3629..4528)
                     /gene="rarD"
                     /locus_tag="PXD94_001976"
     CDS             complement(3629..4528)
                     /gene="rarD"
                     /locus_tag="PXD94_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL6286810.1"
                     /translation="MLKGILLSLSASVLFGCMYYLAIFLKPLAGVSIFGIRMLVTIPF
                     LFLALYLFKKQNEFWVFLQRLKQEPYLLLVILITASIVGGQMWLFLWAPNSGKAIEVS
                     MGYLLMPIIMVAFGKVVYKETLSLNKWLAIVVAFIGVASNILFTGKISIASLFVCTGY
                     PIYFYLRRKFGLSHLHSFVVEILYLLPVACYFISQTDMQYVETQNLNIYYFIALLGLI
                     SGTALISYTLASTILPFNLLGLLGYVEPLLMLVVSFLIGERLANESYLLMICLLISIS
                     FLVLDGMFALRQQRKRKLSVNNE"
     gene            complement(4755..5972)
                     /locus_tag="PXD94_001977"
     CDS             complement(4755..5972)
                     /locus_tag="PXD94_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL6286811.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLRQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            6160..7464
                     /locus_tag="PXD94_001978"
     CDS             6160..7464
                     /locus_tag="PXD94_001978"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL6286812.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIVSIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLHFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVSGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
BASE COUNT         2289 a         1662 c         1488 g         2133 t
ORIGIN      
        1 agttcattaa actgatcacc gcaatgatcc tcccgccatt taatattatt ttgctgtggt
       61 tgttagcatt aatcctcttt tctttaggct ataaatacgc tgcttacttc tttgccttgt
      121 taggtattct gattttatat atcttcagca ttccctatac ttcacagaaa ttaggggatt
      181 cattagtcga agcagagcca tttaccatag agaaatataa aaatgctcaa gcgattgtgg
      241 tgctaggtgg cgggctacga gacagccaag agttattcgg caaactggca attaccggta
      301 ttccgctgga aagaatgcgt tatgccgctt atctgcataa agaaaccggc ttaccgattt
      361 tagcgaccgg aagcagccca aatgggactt ctgaggcaaa ggttatggca gatgaattta
      421 aaatgttctt caatattgag accaaatggc tggaaaataa ggcgaaaacc accaaagaaa
      481 atgcgatttt cagccgtgag attttagaaa aagagaatat tcataaaatc attcttgtta
      541 ccaaccaatg gcatatgaaa cgggctgaaa tgctgtttag ccaacaagga tttgaggtga
      601 tgtctgccag cgtcggttct ggcgatacgc ccgatcatta caaactcaac tttatgcact
      661 ttattccgca agccggtgca atgaatagca atatgctggc attaaaagag tggattggtt
      721 attggaaaga gaaataaccg aagataaata gcagcaaggc aggttttcac ctgccttctt
      781 tttgcaaaaa atcagtcaaa aacaaccgct tgtctaacta atctcttgtg ggtcaatgtc
      841 gatattcagc ctgatattat tcggaatgtt taaactttcc ctttcttgat caaaggcatc
      901 gagtagctgt tgtaatgctc ctcggagagg gtgttgaatc agcaataacc agcggtaatg
      961 tcctgctttc ttcgccattg gggctgaaaa tggtggaggg agctggaagc cttgcccgtt
     1021 ttgttgctgg attttttggc taaaataatc tgctaattgc tcaagcagct gtaaggtttg
     1081 ttgattatct ttgccggtcg ctttgaacaa aacttgcgag gaaaatggtg gcaaactcat
     1141 taattgcctc atttttaacg cctcatcagc aaaagctcgg tagccttgtt ccagcaaggt
     1201 tttaagcagt gggtgatcag ggtaatgggt ttgtagcacc acttcgcctt ttttctccgc
     1261 tcgccctgct ctgcctgcca cctgtatgta aagctgtgct aatttttctt ccgcacgaaa
     1321 atcagttgaa aataacgctg aatccacatt aacaatcgcc accaacgtca cattcgggaa
     1381 atgatgccct tttgccaaca tttgcgtacc aatcaaaatt tggcttttgc cctctcgaat
     1441 agcggtcaaa tggttttcca atgcaccttt gcgagcggta gaatctctgt ctatacgagt
     1501 cacttggtaa tgagggaatt gctgatttag cacctgctca agttgttctg tgccgacacc
     1561 ggtagttatt aaatgggttg aaccacaatg tccgcattgg cggggaatta ccctttgggt
     1621 ggcacaatga tggcaacgca acactttttg cttttggtgg taagtaaaag gcttattaca
     1681 cgcctcgcac tcacaaaccc aaccgcattc gtgacaaagt aataccgggg caaagccacg
     1741 gcgatttaaa aacagcatca cttgattgcc ttgctctaac tgggttttca tcattgaaag
     1801 caagcgctcc gacaaaccgg cagttacccg ttgcgttttt aaatcaataa tctgctgatt
     1861 ggcaagttgt gcattgccgg cccgagcggt tagtgtcagt tcgataaatt tcccgttttg
     1921 cacattgtgc aaactttcaa gacttggcgt tgccgagccc agcacaatcg gaatatttgc
     1981 atttttcgcc ctaaccaccg ccaaatcccg tgcgtggtaa cgccaaccgt cttgttgctt
     2041 aaacgagcta tcgtgttctt catccaagat aatcacgccc aatttttgaa actgggtaaa
     2101 taaagcagag cgagtgccga ttacaatcgc actttctcca tttttcgccc gcaaccacgc
     2161 atttaaccgc tcagtttcgt tcatattcga gtgcaatacg tcaatttcaa cgttaaagcg
     2221 agctttaaag cgttgtaccg tttgcggtgt tagcccgatt tcaggcacaa gcagcagcac
     2281 ttgttcctct cgcttaagca ccgtttcaat taactgcaaa tacacttcgg ttttgcccga
     2341 gcctgttacg ccattgagta aaaagctcgc aaaagtggtt tgtgcattta aacggctgac
     2401 cactaaggtt tgttgtttat ttaagatcag ccgattgtta gtatttacta acggaaattc
     2461 acctagtgag tcttgccacg cttgtgccat aaacggcaca tcaattttct cgatataccc
     2521 tttttcgagt aagcctttcc aagcagatgg gctacaagcg gtcggttttt caaaatattt
     2581 tgcacattcg gcaagctcgg ttaaccgttc cacctgcttt ttggcttttt tattttcacc
     2641 gataatcaga gatttccgcc cttgctccgt caccacaaaa taattcggtt gaactcgctc
     2701 agtggaatcg ccattgcgta attttatcgg taaggctccg gttagcactt caccaatcgg
     2761 ggcgtggtaa taacgagccg cccagttcag cagttgccac atttctgagt caaaaatcgg
     2821 gctagaatcc aacgctgctt taatcggttt gagtttttcg gttggcacat cactagtttg
     2881 cgggaaatcg accacaatgc cgattttggt ctggcgacca aaaggcacaa ccacccgccc
     2941 gccttggcag gcattgaatg ccggcggtag caaataatca aaataacgat gtaacggcac
     3001 attcagtgcc actctgatta atctcacttc tctaaaccaa ttttgattgc aagcccgata
     3061 aatatcgagc cgcagatttt atttaaccaa tttccaaagg tttgattatg ccgtaaaaaa
     3121 cgcccaacag gtgcagcggc taagactaaa atacttaccc aaataatgct ggtggataaa
     3181 aacgttagcc ccaacagcat aaaaggtatt ttccaaatta ctggatacaa actgcggcaa
     3241 taacgccaag aaaaagagca atactttcgg gtttaataga ttggtaatca acccttgacg
     3301 gaatatcttc catagaggaa gtttgtctag attgattttc tctaacacca acggctctcg
     3361 ccacatttta atgccgagat agcagagata aattgcccct gcgtatttca gaaaattaaa
     3421 taaagtgggc gaatgtgcca caatgcctgc aagcccgagt gaaaccgcaa gaatatggca
     3481 taaaatgcct gctagaatgc cgaatgaaga cattaagccc gactttttcc cctcagcaat
     3541 agtgcgagtt aaaatataga tagtatccga acccggtgta agatttagca gaatacaagc
     3601 ggtcaaaaat ccccaataat ttacaatatc actcattatt cacactcaat ttacgtttac
     3661 gctgctgacg aagagcaaac ataccatcta atactaaaaa tgaaatggag atcaacaagc
     3721 aaatcatcaa caagtaagat tcatttgcta acctttcgcc aattaagaaa gacaccacca
     3781 acatcaatag tggctcaaca tagcctaata aaccaagcaa attaaagggt aaaatcgtgc
     3841 tggctagcgt atagctgata agtgcagtgc cactaattaa acccagtaat gcaataaaat
     3901 agtaaatatt gaggttttgt gtttcgacat attgcatatc ggtttgagag ataaaataac
     3961 aagctacagg gagcaaatat aaaatttcta ccacaaagct atgtaaatgg cttaagccaa
     4021 actttcggcg aaggtaaaaa tagattgggt aacctgtgca aacaaataaa cttgctatcg
     4081 aaatttttcc cgtaaacaaa atattactcg ctacaccaat gaaagcaacc acaatcgcca
     4141 accatttatt caaggaaagc gtttctttat aaaccacttt gccaaatgcc accataataa
     4201 tcggcattaa tagatagccc attgaaacct caatcgcttt tccgctattg ggagcccata
     4261 aaaacagcca catctgcccg cccacaatag atgccgtgat taaaataacc agcaataaat
     4321 aaggctcttg ctttaggcgt tgaagaaata cccaaaactc attttgtttt ttaaataagt
     4381 ataaagcaag gaaaagaaaa ggaatcgtaa ccagcatacg aatgccgaaa atgctcacac
     4441 ctgccagcgg ttttagaaag atcgccaaat agtacataca gccgaataac actgaggctg
     4501 aaagagataa aagaatgcct tttaacatag tgataagaaa gtaattattt tttaattttt
     4561 caaatctccc ctacccctct tttctaaaga ggattgtcga gttctaaata aatgaataat
     4621 gttcggaggt aggggcgggt ccagtgcccg cccgcaaatt aatatcgtta gcggacaggc
     4681 acaggacctg tccctactga tattatagaa cttacaaccc tgcttttata aagtggggta
     4741 aagggagatt cgacttagtc ttgacgatag gttttcaaga aattcgcaag gcgaccaatc
     4801 gcttcttcaa tttgatgtgc ataaggcaaa gttaccacac ggaagtgatc cggttttttc
     4861 cagttaaaac cggaaccttg cactaatagc actttttctt gtcgcaacaa atcgtagatg
     4921 aatttttggt catctttaat gccgtacatt tcggtatcaa ttttcgggaa catatataac
     4981 gcccctttcg cttttacaca gctaataccc ggaatttgta ccaataattc gtgcatttta
     5041 tttcgttgtt ccagcaaacg accgcccggc aacacaaatt cattgatact ttgataacca
     5101 cctaatgcgg tttgaatggc gtgttgcatc ggcgtattgg cacataaacg catagaagag
     5161 agcatatcca acccttcaat aaaacctttg gcttgatgct tcggaccgct cagcaccatc
     5221 cagccctgac ggaaaccggc aacacgatac gccttggata aaccgttata agttacggtt
     5281 aatacgtccg gtgcaagagc agcaatatgg tgatgtaccg caccatcata gacaattttt
     5341 tcataaattt cgtcagcaaa aataattaag ttatgctgac gggcaagttc tgcaatttcc
     5401 agcaaaattt gacggctata cactgctccg gtcgggttat tcgggttgat aaccaaaatc
     5461 cctttagtgc gaggcgtgat tttcgattta atatcttcaa gatcagggaa ccactcattt
     5521 tcttcatcgc acaggtaatg cactgcttta ccaccggcta gggttgaagc tgctgtccat
     5581 aacggataat caggcattgg aattagaatt tcatcgccat catttaataa cgcctgcatc
     5641 gacattgtaa tcagttcaga tacgccgttg ccgatgtaaa catcgtttac atccatacca
     5701 cgcatacctt ttgattgata atattgcaca atcgctttgc gggcagaata caaacctttt
     5761 gaatcacaat agccttgtgc ggttggaaga ttacgaatca catccactaa aatttcatcg
     5821 ggtgcttcaa agccaaaagg ggctgggtta ccgatattta attttaaaat tttatgccct
     5881 tcttcttcta agcgaagtgc ctctttatga attggcccac ggatatcgta acgaacctga
     5941 gctaacttat ctgatttggg aaaacgatcc attgtaaact tcctataatg tattatctaa
     6001 ttgtagtctc atcccgctgt tcaaaaaatc ggtaattcgg aatgaatttg taaacattac
     6061 tctaatttgg gaggaaaaaa aagaggaaat acgttaaaat ggagaccttt ttttcaatca
     6121 gctaaaaaca agcggtttct ttttctgatt tttttgcaaa tgcttatttt tcatcaatta
     6181 aaacaagaac ttacaaaaag ttactcaaaa gtgggaagag agcaaggatt ggtcgccatt
     6241 caagcctcta tgccgctatt ggaggcaaat attccgctac tcggttggtt gaaaacacaa
     6301 aaaacaagct accctcactt ttttctgcaa tatcgtgata cagatcagac cattgccacc
     6361 attggcacac ttcgccaatt ttatacgcta gaagctgcac aatcttttat tcaacaacat
     6421 catttcccgt taatcggcgg gcttcaattt gaaggcaata cgcaatttat tttgcctcaa
     6481 ttttcattgg taaaaaatca gcaaaattta accgcttgct tctattttga tagtgataac
     6541 tttgaacaac aagcggttat ttttgaacaa tttcttgcaa atttcagcca aattaccgaa
     6601 ttaggactaa cggataatcc gctcgtttca atgagtgcca ccagccattt tgagcaatgg
     6661 aaagccaata ttgaaaaagc gattgtttct attgagcagg gcgattttca aaaagtggta
     6721 ttggcaaatg cgatgagact tcattttgag caacccattt gtgcttacag cttgctggca
     6781 caaagccaaa aaacaaattt aggttgttac cactttttat gggcggaaag tgctgatgtt
     6841 gcctttcttg gctcaacccc tgagcggcta tatcaacgcc aagggctaca cttttctacc
     6901 gaagccctcg ccggcaccgc tgcggttacc accagtgaag tgcaaaccga actcaatgca
     6961 caatggctgt taagtgatcc caaaaatatc tatgaaaatc aattagtggt tgatgacatt
     7021 gaaaaccaac tcaaagattg cgtgaccgaa tttgcagtat ccgaggcaaa aattaagcgt
     7081 ttgcataatg tgcaacacct acgccgccaa attcaagcaa cgctcaaacc ggacacctca
     7141 gattcagatt gcttaagcaa aatccaccca actgcggcag ttgcagggct tccccgccaa
     7201 aaagcaaagg gttttattac cgaaaatgag ccttttaccc gccattggta tgcaggtaca
     7261 ttaggcgtaa tgggcaacga ggaagccgaa ttttgcgtaa cactccgttc tgcattaatt
     7321 tcacaaaatt caattacgct ttatgccggt gccggcattg tgtcaggctc agatccacaa
     7381 tcggaatggc aagaaattaa acgtaaggca caaggaatgg caaaactgtt ggatgttgat
     7441 ttagtaacaa aaggggaact gtaagctagg ctttcagaac ttaatctctt aattatttct
     7501 ttctatattt tcatttccaa cttcccaaca aaatatatta ttgccaccaa attagtagtg
     7561 aatattccat aa
//
LOCUS       DANLXQ010000121         7554 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.121, whole genome shotgun sequence.
ACCESSION   DANLXQ010000121 DANLXQ010000000
VERSION     DANLXQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.121"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            61..1524
                     /gene="ubiD"
                     /locus_tag="PXD94_001979"
     CDS             61..1524
                     /gene="ubiD"
                     /locus_tag="PXD94_001979"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDL6286813.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQLPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWPGDVAPLVTWGL
                     TITQGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAENPGKPFLVSVAI
                     GADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPASSEIVLEGYIDL
                     EETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPPDEPAVLGEALN
                     EVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMGVWSFLRQFMYT
                     KFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIEHTPIDYLDFASPIAGLGSKMGI
                     DATNKWAGETTREWGTPIEKDDNVVKRIDEIWDSLGI"
     gene            complement(1609..2433)
                     /locus_tag="PXD94_001980"
     CDS             complement(1609..2433)
                     /locus_tag="PXD94_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6286814.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGSFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHNAPERDWNVEELANVALMSRSRFAAYFKQTMGVSPMDY
                     LTNWRLAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            2560..3405
                     /locus_tag="PXD94_001981"
     CDS             2560..3405
                     /locus_tag="PXD94_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL6286815.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLGPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSPHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVITSPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            3862..6804
                     /gene="rne"
                     /locus_tag="PXD94_001982"
     CDS             3862..6804
                     /gene="rne"
                     /locus_tag="PXD94_001982"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL6286816.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARH
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRERRQRRERPNRNAKAENQERKTPQAQTTPAEEKPMRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRIETQEVEVENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQEVPVETAPEVETTNEGRENNRQRRLPRHLRVGNQRRR
                     ENQRNQVISPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            6950..7510
                     /locus_tag="PXD94_001983"
     CDS             6950..7510
                     /locus_tag="PXD94_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286817.1"
                     /translation="MKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEK
                     AAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAA
                     ANDAAQATTDKTEEAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKA
                     VAEKAAEVKVETETKVEEMKKAIESK"
BASE COUNT         2444 a         1593 c         1654 g         1863 t
ORIGIN      
        1 gggtattttt catttacgtt tttctatata cttgcaaaaa aatcgaaaaa tatgaccgct
       61 atgaagtata aaaatctccg tgaatttctc gatttccttg agtctcaggg tgaattagtc
      121 cgtattcagc aagaaatcga tccttattta gaaatgacag aaatcgccga ccgcacctta
      181 cgcaaaggcg ggccggcatt actgtttgaa aacccgaaag gctatcaaat gtcggtgttg
      241 tgtaatttat tcggcacgcc caaacgggta gctctgggaa tggggcaaga agataccaaa
      301 gctctgcgtg agctgggcaa gttacttgcc tttttaaaag agcctgaacc accgaaaggg
      361 tttaaagatt tgatcgggca actgccacaa tggaagcaaa ttttaaatat gccgcctaag
      421 gtactgagca aagccgattg tcagcaagtg gtgatttcgg gtgatgaggt tgatctctac
      481 aaattaccga ttatgcactg ttggccgggc gatgtcgctc cactggtcac ttgggggctg
      541 acgattacgc aagggcctta caaaaaacgc cagaatttag gcatttatcg tcagcaactt
      601 atcggcaaaa acaaactgat tatgcgttgg ctttcccacc gtggcggtgc gttagacttc
      661 caagagtgga aagccgaaaa cccgggtaaa ccgtttctgg tgtcggttgc gattggtgca
      721 gatccagcca ctattttagc ggcagtcacc cctgtgccgg atacgctttc ggaatatgcg
      781 ttcgcagggc tgttgcgagg gcataaaacc gaagtggtca aatctatttc caatgagtta
      841 gaagtgccgg caagtagcga aattgtgctg gagggctata tcgatctgga agaaaccgca
      901 ttggaaggcc cttatggcga ccacaccggc tactacaacg agcaagagta ttttccggta
      961 tttaccgtta cccatattac gatgcgaaaa gatgcgattt atcattctac ctacactggc
     1021 agaccacctg atgaaccggc agtattaggt gaagcattga atgaagtctt tattccgatt
     1081 ttgcaaaaac agtttccgga aattgtcgat ttctacttgc cacctgaagg ctgttcttac
     1141 cgtttagcgg tagtgacgat gaaaaaacaa tatgccggac acgctaaacg ggtgatgatg
     1201 ggagtttggt cgttcctgcg tcaatttatg tacaccaagt ttgtgattgt gtgcgatgac
     1261 gatgtgaacg ctcgagattg gaaagatgta atttgggcga tcaccacccg ttgtgacccg
     1321 gctcgagata cgacactgat tgaacacacg ccgatagatt atctcgattt tgcctcgcca
     1381 attgcagggt taggctcaaa aatggggatt gatgccacca ataaatgggc gggtgaaacc
     1441 acccgagaat ggggaacacc catcgaaaaa gatgacaatg tggtgaaacg cattgatgag
     1501 atttgggatt ctcttggcat ttaaggcaac atttaaccgc ttgtgcaaca taacaagcgg
     1561 ttaaattttt tgacattttt accattcggc tatttgtttc tcatcaggtt acgactcttt
     1621 attttgagcc agccactcgg taggcgaaag ccctaattcc cgcataaaga cacggctgag
     1681 agcagcatta tggctataac cgacttgctc tgccaccaat gctactggta tccctttttg
     1741 taataaatgc tgggcaaccg ctagtcgcca gttagtgaga taatccatcg gtgaaacccc
     1801 catcgtttgt ttaaaataag cagcaaagcg tgagcgagac attaatgcca catttgccaa
     1861 ctcctctaca ttccaatctc gctctggtgc attgtgaata gctaacagca ctttccccag
     1921 ttgcttatct gccaagcctt ttaacaaacc tgtttgaata tacccttttt ccaaacattg
     1981 gcgggcaact ttcaaggtaa aataggcaca taaatgatga atcattgcct gataaccaca
     2041 ccctttctga ttcacttcac taaaaatttg atcggctaat acccccaatt ccggatactg
     2101 tgccaactct aagatcacaa tattgtgcaa ggtatgagtt aatgggttgc gaatcccttt
     2161 gccaaaatca aaattaatac aaaatatatc taacccacta tcatctaacg ggtgcaaact
     2221 atgcaaaata ttggttggtg aaaaaataat cgctgggcgt tcaatcgtaa tcgggcgttc
     2281 ggcagcctga tttaaaataa aacgtccctg acgaacgaaa tgtaaatagc ctttattctc
     2341 ttcattaaaa ctcccgattt ggcataactg acctagatga aataaatccg tgcgaaactg
     2401 aaattgggta aaaagatgag tcaaactatc cacattaagc tccttgggta aagaattgag
     2461 acgaacggtt aaaacaggag aaatgcgatc actataatag caacccgttc ggtaacgcaa
     2521 gttatcatta caatttgtat atctattaat gaggataaaa tgatgtcaca ggaacaattt
     2581 gaagaaatca accaagaacg ccgtcaagcc cttaaccacg gctctcgtat tgcaatggca
     2641 gccagtgtat taggtgcatt aggtattgca tcaacagcaa atgcaaaaca agccagtgca
     2701 gaaaaatcac cttatgccga gccggaacaa tacggtttaa aacgccatgg aatgaccatt
     2761 gatccaaaac gtgtggcatt agtggtagtt gatccacaaa ttgacttcct aggcccgaaa
     2821 ggcgtgatgt ggggcgtgtt aggtgactca attaaagaaa acaacactgt agctaacatt
     2881 gaaagcttat ttaaagcagc aaaagcagtc gatatgccaa cctttgtttc gccacactac
     2941 tattacccga ctgatcacaa atgggaattt ggttcaccgg gtgaacactt tatgcacgac
     3001 agcggaatgt ttgcgagaaa aggtcaatac accatggaag gctttgaaaa ttcaggtgca
     3061 gactttatgc ctgagtacaa accatacatt tttgatggca aaacagtgat tacctctcca
     3121 cacaaaatct ttggaccgga aaccaatgac ttagtgttgc aattacgcaa acgtaaaatc
     3181 gaccaagtaa ttttagccgg tatggctgcc aacttatgta ttgattcaca cttgcgtgaa
     3241 ttaatcgaac aaggttttga agtaacagtg gtgaaagatg caaccgcagg gcctcgtatt
     3301 cctgaaggtg acggctacct tgcagcatta accaactacc gtattatcgc aaatgacctc
     3361 tggaccactg aagaagcagt gaaaaaaatg acggcaaaag cataatattc gctttacaag
     3421 cggttaaatt tagctctttt tttgcaaatt taaccgcttg gatttcacgc ttatctccag
     3481 cactcacact aaatctcatt atttttcctt atcaccccat tcattttcat tatattcatc
     3541 tccacaacaa ataacagcta ccatacaaca attaccttgc ctttttcagg gttaaaatgc
     3601 gataatgact cgttattttt ggcttgaaaa agcaatagaa aattgcaaaa aataaagcaa
     3661 aaataaccgc ttgtaataga gataaacaag ccttaagtga ttgattttgc agtttcttta
     3721 agacaagaca aagtaaacgt aagacaacca tcaatgcact atcgggaatg aagacagtcg
     3781 tgagatagac attaaatcca ttcccttatt aatgggtcaa aatgaccgtg taagacaaaa
     3841 acaaaataat gagaaaacac aatgaaaaga atgttaatca atgcaactca aaaagaagag
     3901 ttgcgtgttg ccctcgttga tgggcaacgc ttatttgact tggacattga aagcccgggt
     3961 cacgaacaga aaaaatccaa tatctacaag ggtaaaatta cccgtgtcga accaagcctt
     4021 gaagctgcat tcgtagatta cggtgcagaa cgccacggct tcctaccttt aaaagaaatt
     4081 tcccgagaat acttcccgtc agactatgtg tttaacggtc gcccgaatat caaagatatt
     4141 attcaagaag ggcaagaagt gattgttcaa gtcagcaaag aagagcgtgg taacaaagga
     4201 gcagccttaa ccacctttat ttcgcttgcg ggcagttact tagtcttaat gccgaacaac
     4261 cctcgtgccg gtggtatttc ccgccgtatt gaaggtgatg agcgtttaga actcaaagag
     4321 gctttagact gcttagatgt accggaagac gtgggcttaa tcgttcgtac cgccggtgtc
     4381 ggcaaatcgc cggaagagtt acaatgggac ttaaaagtct tgttacacca ctgggaagcg
     4441 attaaaaaag cggcggaatc tcgcccagct ccgttcttaa ttcaccaaga aagtgacgtg
     4501 attgtgcgtg caattcgtga ctatctacgc cgtgatattg gcgaaatctt aatcgacaac
     4561 aaaaaagtgt tcgagaaagc caaaaaccac attcgccttg ttcgcccgga tttcatcaac
     4621 cgtgtgaaat tatacgaagg cgaagtacca ctattcagcc actatcaaat tgaatcgcaa
     4681 atcgaatcgg cattccaacg tgaagtgcgt ttaccttccg gtggctctat cgttattgat
     4741 gtaacagaag ccttaacagc aattgatatt aactcctcac gttcaacccg tggcggcgat
     4801 attgaagaaa ccgctcttaa caccaactta gaagcggcag atgaaattgc ccgtcagtta
     4861 cgtttgcgtg acttaggtgg tttgattgtg atcgacttta tcgatatgac accggttcgc
     4921 caccaacgtg aagtagaaaa ccgtattcgt gaggcgacac gccaagaccg tgcgagaatc
     4981 caatttgccc gtatttcccg tttcggtttg ttagaaatga gccgtcagcg tttaagccca
     5041 tcattaagtg aggcttcatc gcatatctgc cctcgctgtc agggaactgg taaagtacgt
     5101 gataatgaat caatcgcact gtcaatctta cgtttattag aagaagaggc aatcaaagaa
     5161 aacactgcac aggttcacac cattgtgccg gtgcaaatcg ccagctactt actcaacgaa
     5221 aaacgtaaag cgatttccag cattgaaaaa cgccacgatg tacaaattgt tgtagtaccg
     5281 tctgaaagta tggaaacgcc gcatttcagc gtatatcgcc tgcgtgaaaa tgaaattact
     5341 ccaacgttaa gctacgattt aaccaagcat tacgaagctc gtcatgaaga agaacaccca
     5401 catcatcacc atcaagaaga tgtgctggtt acccgtaacg agccggtgat tacggtagag
     5461 tcagtgttac aaagctcgga gctgaatctc caaccttcgc caacaccaac cgaaccacca
     5521 aaaccatcgc tgttaagccg cttaattgcg accattaaag gctggtttag tgaagaaaaa
     5581 gtggaagaaa aaccgaagcc gaaagcacgc aatcagcgtg agcgtcgcca acgccgtgaa
     5641 cgcccgaacc gtaatgctaa agcggaaaat caagagcgta aaactccgca agctcaaact
     5701 actccagcgg aagaaaaacc gatgcgtagc gagaaaaaag cacgccgagc ggtagtggaa
     5761 gaagtttccg gtgtaaccga gcaagagagt cgtagcaact cccgtgaggt tcaaactgaa
     5821 cgtcgccaac gccgtgatct acgtaaaaaa gtgcgtatcg aaacacaaga agtagaagtg
     5881 gaaaatctaa cacctgctgt tgaggaaacg gtggcaaaac aagttgaaaa tgtgcaaaaa
     5941 aatcaacaaa ttcaaccgct tgcaacccaa gaagtacctg ttgaaacagc tcctgaggta
     6001 gaaacaacga acgaaggacg tgaaaataac cgccaacgcc gtttacctcg ccatttgcgt
     6061 gtgggcaatc agcgtcgtcg tgaaaatcaa cgcaatcagg ttatttctcc tatgcctcta
     6121 acttcagctg ttgcttcgcc tgagcttgcg agcggtaaag tttgggttca ggttacctct
     6181 actgtcaaag tagaggaaaa agcatcattc ttatctgttg atcaaatgct tgaacagcaa
     6241 gaaaatcagc ttgttgagac agagaaaaaa tcagaagaga agaagcttca tacagttagt
     6301 ccgctaggtg gctttgtagc acaagcaact aaccgtgatt tggttaagga gaagcaagaa
     6361 cagctttcca aaccgaaaga agaaaaagtt gcttctgaag acgtaaaacc agtagaggta
     6421 aaagagcata ttgctccatt tggtagtgtt gttgctcagc attctgaacg tgatttagtt
     6481 aaagagaaaa atgagcgagt cagtaaattc caatcgcaag caacggttgc accagcgatt
     6541 gtggaagagg ttactaaccc gattgaagtg ccaaaatcaa cttacgaaag cgattatcga
     6601 tttaacggca gattaggtac attctctcgt gttgagcata cccaagccgc tatgaccatt
     6661 gcttctgcac caagtgacaa gcttgaagta gtcgaaatta gccaatggca aaactctaaa
     6721 tactatttct atggcaaagg gtttggcgga tataccagcg cagtgagcca agtgcagtca
     6781 gcgcctcgtt caaccaatgg ttaagtacaa ttaattggtt taaatgccta ttagttataa
     6841 ctaataggca tttttttatc aaagtatgaa ctttctatca atttttctgc ctaagtaaac
     6901 gcactttatt acaaagtgcg ttatttttag tttatttaag gaactcggta tgaaaaaatc
     6961 atttttatca ttattagtgt taggtacaac tatggcactt gctgcttgtg atcaggcaaa
     7021 agaagccgcg caatcaacta cagacaaagc ggttgaagta aaagaagctg tagctgaaaa
     7081 agctgcagaa gttaaatcgg ctactgaagc gaaggttgaa gagttgaaag cggcagcaaa
     7141 tgatgcagct caagcaacag cagataaaac agaagaggca aaaaaggcag tggcagagaa
     7201 agccactgag ttaaaaacca caactgaagc taaagtggaa gaactaaaag cagcagcaaa
     7261 tgatgcagct caagcaacaa cagataaaac agaagaggca aaaaaggcag tagcagagaa
     7321 agctgctgag ttaaaagcca caactgaagc taaagtggaa gaactaaaag cagcagcaag
     7381 tgatgcgact caagcaacag cagataaagc agaagaagct aaaaaggcag tagctgagaa
     7441 ggcagcagaa gtgaaagttg agacagaaac aaaagttgaa gaaatgaaaa aagcgattga
     7501 aagcaaatag tgctttttag tttctctgta gctatcgaaa cacgctaaaa ataa
//
LOCUS       DANLXQ010000122         7546 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.122, whole genome shotgun sequence.
ACCESSION   DANLXQ010000122 DANLXQ010000000
VERSION     DANLXQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.122"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(34..1869)
                     /gene="glmS"
                     /locus_tag="PXD94_001984"
     CDS             complement(34..1869)
                     /gene="glmS"
                     /locus_tag="PXD94_001984"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL6286818.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVKALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKAEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPVAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(1917..2678)
                     /locus_tag="PXD94_001985"
     CDS             complement(1917..2678)
                     /locus_tag="PXD94_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL6286819.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLATELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELKWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(2835..3035)
                     /locus_tag="PXD94_001986"
     CDS             complement(2835..3035)
                     /locus_tag="PXD94_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL6286820.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
     gene            3260..4294
                     /locus_tag="PXD94_001987"
     CDS             3260..4294
                     /locus_tag="PXD94_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6286821.1"
                     /translation="MKLKKLSLALSTLLLGSAFANSALAEGRLTVYCSATNELCEQEV
                     QAFGKKYNVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFRDPAKLKGNYSSAVYMGILGYGVNLDRIKKLGIEKVPSTWEDLLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLLGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETAIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAESSPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            4362..6292
                     /locus_tag="PXD94_001988"
                     /pseudo
     CDS             4362..6292
                     /locus_tag="PXD94_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            6390..7439
                     /gene="fbpC"
                     /locus_tag="PXD94_001989"
     CDS             6390..7439
                     /gene="fbpC"
                     /locus_tag="PXD94_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL6286822.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGEDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEERLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLANAEQFELPDGEYLVG
                     IRPEAIHLAEQGNISQLCKIKNAVYMGNHWEIIAEWEGKDLLVNTKPENFYPHIKQAY
                     VHFIEQGVFLLKKEL"
BASE COUNT         2060 a         1708 c         1530 g         2248 t
ORIGIN      
        1 aaaaccccaa gatatcttgg ggtttaacta acattactct acagtcaccg cttttgcaag
       61 gttacgaggt tggtcaacgt ccgttccttt gattaaggca atgtgatatg aaagtagttg
      121 taacggcacg gtgtagaaaa tcggtgcggt tacttcatca actttcggca ttacaatcgt
      181 tttgaagcct tctgcttctt caaaacctgc atcgtgatcg gcaaacacat ataactgacc
      241 gcctcttgca cgcacttctt caatattgga tttcactttc tcaagcaagt cattttccgg
      301 tgctaccacc actaccggca tatcgctatc aattaatgct aacgggccgt gttttaactc
      361 gcctgcggca taagcttctg cgtgaatata tgaaatctct tttaatttca atgcagattc
      421 catcgcaacc gggtaatatt ctccacgacc taagaacaat gtgtggtgtt tatccgcaaa
      481 atcttccgat aatttttcga tttgtttatc gaacactaac gcactttcaa tttgtgctgg
      541 taagcgttgt aaagattgca cgatatggtg ttcttgctct tcactcattc tgccttgtaa
      601 gcgtccaatc gccacattta ataacaataa gcaagttaat tgagtagtga atgctttggt
      661 tgatgctaca ccaatttcaa caccagcttt ggttaagaat gcaaaatcgg attcacgcac
      721 taatgaagaa cttgccacat tacacaccgt cattgccgcc atatagccag attctttcgc
      781 taaacgcagt gcagctaacg tatctgcggt ttcacctgat tgtgaaagcg tgattaataa
      841 gctgttcggg cgagtaacga atttacggta acggaattcg gaggcaattt ccacatcaca
      901 gctcacgcct gcaatggatt caaaccaata acgtgaaacc ataccagcat tgtaagaggt
      961 tccacaagcc acaatttgca cgtgttctac tttcgataaa atctctgccg cattcggtgc
     1021 aatggctgaa atatctactt taccgtcttt gatacgacca tctaaggtat tcataatcgc
     1081 aaccggctgc tcaaaaatct ctttttgcat atagtgacga tattgtcctt tatctgccgc
     1141 gtctgcctca aaattgcctt cgtgaatttc acgctctgcc ttgttgcctt cacggtcata
     1201 aatatccact gtgcgacggg taatttcggc tacatcacct tcttctagat aagcaaaacg
     1261 gcgagttacg cttaataatg caagtgggtc tgaagcaagg aagttctcgc ctacaccgta
     1321 accaatcact aacgggctac ctgagcgagc cacaattaaa tgctccggct cttcttcatt
     1381 taataccact gtgccgtaag caccacgcag ttgcaccacg gttttttgta ccgcttccaa
     1441 taatgaagaa gcagaacgca attcccattc aactaaatga gcaatgactt cggtatcggt
     1501 ttgagattgg aacacataac cacgctcttg taacactatt tttaactctt cgtagttttc
     1561 gataatcccg ttatgaacta ctgcaatttt gccactgcgg tgtgggtgag cattagtttc
     1621 tgacggctcg ccgtgcgttg cccagcgagt gtgtgcgatc ccagtgccgc ctaatagtgg
     1681 cttagtgtct aacgcctcat ctaaggcttt gactttaccg acacggcgta caatttgcat
     1741 tgttttatca tctgcattta atactgctac accagcagag tcatatccac ggtattctaa
     1801 gcgatgtaaa ccatcaacca agatttctgc gatatcacgt tgtgctactg cacctacaat
     1861 tccacacata aatttgcttt cctatgttta agtttaaaaa ttcatttgag ctactgctat
     1921 tttgcaataa ccacttcgac acccattttt tcgatttgtt ctttcgtttc aaaacttaat
     1981 tgactgtcgg taataatttt ggtaatattt tgccatttca attcaatatt cggcattttc
     2041 cgaccgattt tttgcgattc aattaacaca atcacttcac ggctcacttc tgccatcacc
     2101 tgacttaagc caactaattc attaaaggta gtcgtgcctc gagccaaatc taaaccgtcc
     2161 gcaccaataa aaagctgatc gaaatcatat gaacgcaata cctgttctgc tactttgcct
     2221 tgaaaggact cagaacggct atcccaagtg ccgccggtca tcaacactgt tggttcgcat
     2281 tcgagggaag tcaattccgt cgctaaattc agcgaatttg tcatcaccac caagccggtt
     2341 tggttcaatg cttttaccaa agctccggtc gtgctgccgc tgtcaataat gatgcgatta
     2401 tgatcacgaa tacaatcacg tgcggcttga gcaatcattt tcttttgaat cgaaagttgc
     2461 tcaataaacg cttcttccat catttccgat ggaattttta ccgccccacc atacttacgc
     2521 aataaaaaac cactttcttc caaagcggtt aaatcttttc taatcgtaac ttcagacgtc
     2581 tcaaagaaat gagcgagttc atctacactc acttcatttt tctcttgtac catttgaaca
     2641 attaaatggc ggcgttgttg cgtgttacgt tttgacatta taatcttcac ttaaatttcg
     2701 tttcaaaact tttgcattat aaagaaagct cgcgaaaaga aaagcagaaa atgcaaaatt
     2761 tacaaaatct atacaaaata aaaccgcttg taatattgat ttacaagcgg ttgatttctt
     2821 gaaatttttt gcaattattt gatttggcta ccaatcacgc caccaagtgc ggcaccacct
     2881 aaagttgcac cggtagattc accaatcgcg tgaccagcca caccgccaat tgctgcaccg
     2941 attgcagtat tacgttgcgt ttttgtcata ttgttacagc cagacacagc aaccattaat
     3001 accattgcac caacaacttt caaaatagat ttcataataa tccctcataa ttaatcaatt
     3061 tcaaataagc caatattctt ggctatgcta taagatttaa tttatttaga aaggttcaac
     3121 tactaccacc tatgaattgc aaattttttc acaatttagg ccgcttgttg attgaactgt
     3181 gagcttgctc acagaatttt atggtatttc ttgctatatt gtatagcaat ctatgcgata
     3241 actcattaag gaaataaaaa tgaaattaaa aaaattatct ttagcactct cgactttatt
     3301 actcggttca gcctttgcca actctgcttt agcggaaggt cgtttaaccg tttattgtag
     3361 tgccactaat gaactttgtg agcaagaagt gcaagcattc ggcaaaaaat ataatgttaa
     3421 agtagcattt gtgcgtaatg gttcaggcag tacattggcg aaaattgaag cggagaaaaa
     3481 caatcctcaa gctgatgtat ggtatggcgg tactttagat ccacattcac aagctgcaga
     3541 aatgggttta ctcgagccgt ataaatcacc gaatctcgaa caaattatcg aaaaattccg
     3601 tgatccggca aaattaaaag gcaactactc ttcggctgtg tatatgggga ttttaggtta
     3661 tggtgtgaat ttagaccgaa tcaaaaaact cggcattgaa aaagtgccta gcacttggga
     3721 agacttgctc gacccacgcc tagccggtga aatccaaatt gccgacccac aaagctccgg
     3781 cacggcttat actgcgattg cgaccttcgt acaactttta ggagaagaaa aagccttcga
     3841 ctatttccgc aaattacaca aaaacatttc acaatatacc aaatccggca tcacaccggc
     3901 acgcaatacc gcccgtggtg aaaccgctat cggtatcggt ttcttacacg actatgcgat
     3961 tgagaagaaa aacggggcta acattgaaat gaccgcacca acagacggca caggctacga
     4021 attaggtggt gtgagtatcc tcaaaggtgc aagaaatctt gataatgcaa aactgttcgt
     4081 ggattggtcg ctttctaaag aagctcaaga actctcttgg caaaaaggca atgcgttcca
     4141 agtattaacc aacaccaccg cagaatcttc cccttatgct ctagatccga aaaacttaaa
     4201 cttaatcaac tatgatttcg agaaatacgg ctcgagcgaa gagcgtaaac gcttgattga
     4261 taagtgggta aatgaagtaa aattagcgaa atagtgttaa tctttccact gaggggataa
     4321 gcaattatcc cctctctaat tataagaatc tgtgaggtgt tatgcagaat ttttcctttt
     4381 cccgaccgct tgtttgggtg cttatcgggt tactcggttt tctcttgtta ccgtctaaag
     4441 ccttagatta tgggctgttt gactcaacct ctgatgaact gctcgccgca atgggctggg
     4501 cgtgctgttc gcctttattt tcctgtcagc gattgtgctg aaaatcagcc tcggttatgc
     4561 ggtgttgccg ttgattattg ccattatggg gcttgcgacc ccaaatttag caacgctcaa
     4621 agtaatgcag ggtgaccgct ttattatcgg ctcgttactg agcattattc tgctgatttt
     4681 cttttttatt gtgtatccga ccatcgccat ttttgtgtcg atgttctaca acggctcgga
     4741 atttgtgcca agccaagtgc tgacaatttt gcaacaacct tatgtcgttc gagtcatcgg
     4801 taactcgctc tcggttgccg gcacggtggg cgttctctcc accctattcg gcttagcctt
     4861 tgctctctac accactcgga ttgctaaacg ctcggcgttt atcgggcgga ttttctcgat
     4921 tttacctatc gtcaccccgc cgtttgtggt cggtttaggc gtaaccctga tgcttgggcg
     4981 ttccggctat gttactgaat ttttagtgga ttactttggc tttgccaaca attggctgta
     5041 cggtttcacc ggtattgtga tagcccatac cctcgcgctc acgccaatgt cgtttatgat
     5101 tttggaaggg gcattgaaat ccattcaccc atcggtggaa gaagcagctt acaccctgcg
     5161 ttccaaccgc tatcaagcct tttttaacat tattttccca ttgttaaaac cggcattggc
     5221 aaactccttt ttagtggtgg caattcaatc cctcgccgat ttcagtacgc cgttagtatt
     5281 gggcggtagc tttgatgtga ttgcaaccca aatctacttc tacattgccg gttctcaact
     5341 ggattatgcc tctgctagca cgcttggggc aattttgttg gtgttctcct tagccatttt
     5401 cgtgatccaa tacctctgga tcggcaatcg ctcttatgtg acggtgtccg gcaaatccta
     5461 tcggggtgat gtccaagact taccaagcgg tatgaaatgg ctgattattt gcaccttatt
     5521 gttctgggga accttcaact tagtgctgta cggcagtatt ttctacggca gtttcaccgt
     5581 gaactggggt gtggattata ccttaaccct tgataactac atcaagctat tcgggcaagg
     5641 gtttagcgat ggcggtttcc cttcgttaat tcaaaccgtg attttcgctg ccgctgccgc
     5701 cccgattact gcactattcg gcttgctgat cgcctatgtg acagttcggc gtgatttcaa
     5761 aggcaagaaa accctggaat ttttaaccct gctctgcttt gccgtaccgg gaacggtggc
     5821 aggtgtttct tacattctcg cctttaacga tgccccgatt tacctaaccg gcaccggtat
     5881 gatcatcatt ctctcaatgg taatgcggaa tatgcctgtg ggtatgcgag cggcaattgc
     5941 cggcttaggg cagttggata aatcgctgga cgaagcctcg ctctcgctga aagccggttc
     6001 gttcaaaacg atgctgtttg tggtgttacc gctactcaaa ccggcactgc tctccgccct
     6061 cgtcaccagc tttgtgcggg caatgacgac tgtcagtgcg attgtattct tagtcaccgc
     6121 cgacactcgt gtggcaacct cctacattct caaccgtgtg gaagacggtg aatacggcat
     6181 tgcgattgcc tacggctcaa ttttaattgt ggtgatgatg gcgattatcc tgatttttga
     6241 ctggattgtc ggcgacaccc gtatcgcccg ttctaaggcg aaaaaaatgt aaaaagacat
     6301 ctactctctc cctttatggg agagagacag aaaaatactt cgttcagaag tatttttcag
     6361 agagagggat ataattaaaa ggacaatata tgaacaacga tttcttagta ttaaaaaacg
     6421 tcaccaaatc tttcggcaag gtgacggtca ttgataatct caacctcagt attccgcaag
     6481 gtaaaatggt gacattactc gggccgtccg gctgtggtaa aaccaccgta ctgcgtttag
     6541 tggcagggct ggaaaacccg acttcggggc aaatttttat tgatggcgaa gatgttaccc
     6601 aaagttcgat ccaacaccgt gatatttgca ttgtgttcca atcttatgcg ttatttccgc
     6661 atatgtcgat tggcgataat gtcggctacg gcttgaaaat gcagggcgta ccgaaagaag
     6721 aacggctgca acgagtgaaa gaggcgttgg aattggtgga tttaggcggc tttgaagatc
     6781 gctttgtcga tcaaatctct ggcggacaac aacaacgtgt cgccctcgcc cgtgccttaa
     6841 ttctgaaacc gaaagtgcta ctgttcgatg aaccgctgag taaccttgac gctaacttac
     6901 gccgttcaat gcgggagaaa atccgtgaat tacagcaacg tttaaacatt acttcccttt
     6961 atgtgactca cgatcagtcc gaagcctttg cggtttcaga tgaagtaatt gtgatgaaca
     7021 agggaaaaat tatgcaaaaa gctccagcca aagagttata ccttcgccct aattctttat
     7081 tcttagcaaa ttttatgggt gaaagcagca tttttgaagg cacgatagct ggcaaccaaa
     7141 ttaatataaa tggatatcaa tttacgttag caaatgctga acaatttgag ttacccgatg
     7201 gggaatactt agttggcatt cgtccagaag ctattcattt agctgaacaa ggaaatataa
     7261 gtcaactctg taaaattaaa aatgcagttt atatgggaaa tcattgggaa attatagctg
     7321 aatgggaagg gaaagattta ttagtcaata ctaaacctga aaatttttac cctcatatca
     7381 aacaggccta tgtacatttt atcgaacaag gtgtttttct tttaaagaaa gagctataac
     7441 gaatttatac taaattaaga ggaaggtagt tataactatt tagcaatttt gagaaaaata
     7501 ggccgcatgc aaaatctctt gatgacaagc ggtcttttct cgccga
//
LOCUS       DANLXQ010000123         7530 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.123, whole genome shotgun sequence.
ACCESSION   DANLXQ010000123 DANLXQ010000000
VERSION     DANLXQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.123"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1995
                     /locus_tag="PXD94_001990"
     CDS             <1..1995
                     /locus_tag="PXD94_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6286823.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAGSFGRGLMGAGVGIAAGAAMARQPARNQMEFDRQLAMVANTAFSDRDVKGRI
                     DGKRELLDAVQKAVSVGGGSKEEALSSLDALLASGAVSTETALNLLPTLQKGAVATGA
                     SANDMAQIAISSMQQFGIKEEDIGRVLDMAVAAGQAGSFELADMASWLPQQMAAAKQA
                     GLSGIEGFERLLIANQQARVTAGTSNEAGNNLVNLLGKITAKETNERFKNIEYKDPKT
                     GKTKAIDFAKSMEHYKGKGQDSLQAFMSIMDDVVGSDKRYQELQAKLKTAKGEEQKRL
                     FNELTNLVEGTAIGEIISDRQALMALLGIKNNVQLGEKVQKDIQNSEGAVETSHAVIR
                     DTNAHKVESLKNTTEFAQMKNFEKVNGVLGTLAELLNKYANEYPNLTQFLSGATDAVK
                     IFGAALAASSVLDLLTGKKDKGGLIGDVLDKSSKASGGKGKVGKLLSVGGSFASVAGV
                     ATMLHGDQAPMSEERKAELRDPNYQARKMYEYKKEILGSRFHSFFRSNETLEQYRKDV
                     SAYEAKQKNSNTMGAVQGVLSTIPSLQELRPKVDSVTQTLSTYQADFQAFGATISAGI
                     EAGLASQSHTIANQITVELDGSVVAEKVAEYQFNFNKRMA"
     gene            1995..3287
                     /locus_tag="PXD94_001991"
     CDS             1995..3287
                     /locus_tag="PXD94_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HDL6286824.1"
                     /translation="MSGWTMPIQQASFKGVRFDVLAVDDSFERAVVEHAYPFVNGADL
                     EDMGLNTQTIRLQAVFFGSSYYTDYIRLLSVLQQKGADVLVHPIRGRLPNMLLVSANL
                     HTDAENINYVALDLTFKEATEMQPIFAFEDSLISKIDKLLLDLENLFDDGLRFWDSVG
                     VFDLKSRLLGLWGALFASFEAVRNLFDLDKKKYSIASSTSQRTYLRDGSGALNQLKEM
                     VDSGIQGRADLTTLSFKSQLLNITDAIDNTQAIVSKVARDEGFNRSKTVKLLGSDVVE
                     LGIMLDLIATTKLTKVLTELVEEREEELLIGDLELINQIVREHWLNIINKLREQQKNT
                     QKGVPSDKTKVIYETAEKLINNIRNVAHQFTALVLAVINKKPPLTVRMVMDSGTIHKV
                     AHAFYGDYERADELLRLNPHIRQPNFIERGTLLNCYWE"
     gene            3290..4417
                     /locus_tag="PXD94_001992"
     CDS             3290..4417
                     /locus_tag="PXD94_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6286825.1"
                     /translation="MENEIVVEIDGSQHKNWKSYDIDSDFLIPADSFNFELGKSSNIE
                     VLPNYSGKTAIVKINGKTVLTGIVDTTHHTISKSGRGFSLNGRDKAAILVDCSAPITN
                     VQGLSLLDAVKKIVTPLGIKEVQLKAEKNPTLSKVDIDVGESAWEAIMRCANSAGLHC
                     WFDPKGVLIVGGADYAQPPVATLYCVKESGDSRKNNFVDATLSYDVSQSYSEVTFLAQ
                     KHGKDSDNAKHDFKWVYKNEEMELYKPKTIVLGDVENLEALMKQAKKQVSDWQLESFN
                     LTIIVPDHKTKDGTLWQAGQRVHVVCEEYDLDGIFFLMGRRFRLSRTGGTTTELRLKL
                     DGVWTPDAYQAKAQEARKRKGKKGRKKKEPVEYVGSWELGK"
     gene            4419..5081
                     /locus_tag="PXD94_001993"
     CDS             4419..5081
                     /locus_tag="PXD94_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL6286826.1"
                     /translation="MMRKLAKQTKEVAKGVQDSVRAAFRGVLNLVKSGSDIQQVQVSA
                     LADETIQDLELMQHFGFTSVPPAGTQAVVIPLGGKTTHGIIVATENGSFRVKNLKNGE
                     TAIYDSSGSTIILKNGRVIDIECDSFNIKCKQYSVTASGGANFNTPKLETDQQFVSQG
                     QISGNGGMVVQGGSGASFTGSLTQQGGSISTDGDVVASGKSLVTHQHNEQGDGRPTSA
                     PI"
     gene            5200..5550
                     /locus_tag="PXD94_001994"
     CDS             5200..5550
                     /locus_tag="PXD94_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDL6286827.1"
                     /translation="MDREISPLTRDYTSENITTLQNAVYIRLTTPKGSWWADGRVGSL
                     LHLIQREKDLSRVGLLAQQYAEEALQPIIDDGRAKSITVTHEQPKDGSLNLLISVIDN
                     RGVKFEFKHPVKLV"
     gene            5563..6624
                     /locus_tag="PXD94_001995"
     CDS             5563..6624
                     /locus_tag="PXD94_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL6286828.1"
                     /translation="MYQIPTLEDVRREILRDIQSLEPTADIEVDSDYFVRASALASCV
                     VGLYAHQNWIVRQYFPDTADSEYLEMHAKLRNLYRKNATYASGSLKVFGTAGSVINEG
                     LQVKFGERFYVTKSSDVIGDDGSATVRVISMATGASSNVKSESKAQLTAAPAGVSSDC
                     LLLPDVTGGTEVESDAALLARLLEQIRRPPSGGNKNDFRQWAESVDGVTSAFVYPLRR
                     GEGTVDIAITSGDTVPSDEIVRATQAYIDEVRPVTAKSVYVLKPVEKRVNFNIKVQLD
                     TDTTLDGVKAEIENALENYFLSLKPADTLIISQIEAVISDLVGVVDREIVQPTRNQTI
                     DAYREMGWFRLGDVTVELM"
     gene            6624..7214
                     /locus_tag="PXD94_001996"
     CDS             6624..7214
                     /locus_tag="PXD94_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL6286829.1"
                     /translation="MVTINSKHGKALGGLLPPVSYDPNGRWLALSLEVEGRELDRIDA
                     RAKTLIDAVDVSTGIFIDDWERVCGLTANSSLPLETRIERVIAKLNQLGGLSIGYITQ
                     VAKELGYCIKISEPQPFIAGLSRAGDMLWHKDIMWTFFVDICNPTDDYKRFRASVSMA
                     GDALMGRYKDPILESLLEEVKPAFSRVWVRYLGDIE"
     gene            7211..>7530
                     /locus_tag="PXD94_001997"
     CDS             7211..>7530
                     /locus_tag="PXD94_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6286830.1"
                     /translation="MKQVMNPINTPTQRFKDGNPATGEYGTIVTAEFLNNVQDSVINT
                     QQELHSVLAEAGIEANDEQVNQVAKAIKKIAGDATRDNFNALANPDGYKHIGRCKSVA
                     ELRT"
BASE COUNT         2220 a         1395 c         1905 g         2010 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctcgcaa
       61 aatgagctac gccgtgcctc agaagcgact aagcaacgag tccgagagtt aaatgctgaa
      121 ttgggtaaaa ccagttttgg ggataaggca ggaagttttg ggcgtgggtt gatgggtgca
      181 ggggttggaa ttgctgcagg tgcagcaatg gctcgccagc ctgcaagaaa tcaaatggaa
      241 tttgatagac agcttgcaat ggtagcgaat accgcctttt cagatcgtga tgtgaaaggg
      301 cgtattgatg ggaaaagaga gttacttgat gccgtacaga aagcagtaag tgtaggcggt
      361 ggctcaaaag aagaagcgtt gagctcacta gacgctttac ttgcctcagg tgctgtttct
      421 accgaaaccg ctctcaattt actcccaacg ttgcagaaag gtgcggtcgc aacaggggca
      481 agtgcaaatg atatggctca aattgctatt tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg gtcgagtctt agatatggca gttgccgctg ggcaagccgg tagctttgag
      601 cttgcagata tggcaagttg gttgcctcaa caaatggccg ctgcaaaaca agctggctta
      661 agcggcattg agggatttga gcgactttta attgcaaacc aacaggctcg tgtcactgcc
      721 ggtacaagta acgaggctgg taataattta gtgaatctgt tgggtaagat tactgctaaa
      781 gaaactaatg agcgttttaa gaatattgaa tataaagatc cgaaaactgg caaaaccaaa
      841 gcgattgatt ttgcaaaatc tatggagcat tacaagggaa aagggcaaga ttcattgcag
      901 gcttttatgt ctattatgga tgacgttgtc gggagtgata aacgctatca agaactacaa
      961 gctaaactta aaactgctaa aggcgaagag cagaaacgct tattcaatga attaacaaac
     1021 ttagttgaag gtacggcaat tggagaaatt atttctgatc gccaagcatt aatggctcta
     1081 ttagggataa agaataatgt tcaattaggt gaaaaagtac agaaagatat tcagaatagt
     1141 gaaggagctg ttgaaacctc tcacgctgtt attcgggata ctaatgctca taaagttgaa
     1201 tcattaaaaa atacaactga atttgcacaa atgaaaaact ttgaaaaagt aaatggagtg
     1261 ttaggcacat tagctgaatt actgaataaa tacgccaatg aataccctaa cttaactcaa
     1321 tttttaagtg gtgcaacaga tgccgttaaa atttttggag cagctcttgc tgcatcaagt
     1381 gtgcttgatt tattaaccgg taaaaaagac aagggcggac taattggtga tgttttggat
     1441 aaatcatcaa aagctagtgg aggtaaaggt aaggtaggta aattgctttc ggttggtggc
     1501 tcttttgcta gtgttgcagg cgttgccaca atgctacacg gcgaccaagc cccgatgagt
     1561 gaggagcgaa aagcagagtt aagagatcct aattatcaag ctcgtaaaat gtacgagtac
     1621 aaaaaagaga ttttaggtag ccgtttccac tcgttttttc gtagtaatga aacccttgag
     1681 caatatcgga aagatgtgag tgcttatgag gctaagcaaa agaatagcaa tactatggga
     1741 gctgtacaag gggttttaag taccattccg agcttacaag agttacgccc taaagttgat
     1801 tcggttactc aaaccctatc cacctatcaa gccgactttc aggcttttgg ggcaacgatt
     1861 tcagccggta ttgaggcagg gcttgcctcg caatctcata cgattgcaaa tcaaatcacc
     1921 gtggagctgg acggttcggt ggtggctgaa aaagtggctg aatatcagtt taattttaac
     1981 aagaggatgg cgtaatgagc ggttggacaa tgcctattca acaggcgagt tttaaaggtg
     2041 tgcgttttga cgtgcttgca gtggatgata gctttgagcg agcggtagtt gaacacgcct
     2101 acccttttgt aaatggggct gatttagagg atatggggct aaacacccaa actattcggc
     2161 tgcaagcggt cttttttggc tcaagctatt acacggacta tatccgctta ctttcagttt
     2221 tgcagcaaaa aggggctgat gtattagtcc accctattcg tgggcgttta ccgaatatgt
     2281 tactcgtttc ggcgaatcta cataccgatg ccgaaaatat caattatgtt gccttagatt
     2341 tgacctttaa agaggcaacc gaaatgcagc cgatttttgc ctttgaagat tcgctgattt
     2401 cgaaaatcga caagctgcta cttgatctcg aaaacctgtt tgatgatggc ttgcgttttt
     2461 gggatagcgt tggggtgttt gatctgaaat ctcgtttgct tgggctttgg ggggcgttgt
     2521 ttgcctcgtt tgaggcggtg cgtaacctgt ttgacttgga caagaaaaaa tacagcattg
     2581 cctcatcaac ctcacaacgc acctatttaa gagacggttc gggggcattg aatcagttaa
     2641 aagaaatggt ggatagcggt attcaggggc gtgctgattt aacgacactc tcgtttaaat
     2701 ctcagttact caatattact gatgcgatag ataacactca ggcgattgtg agcaaagtgg
     2761 ctagagatga gggttttaac cgctcaaaaa cggttaagtt acttggctct gatgtggttg
     2821 agcttggcat tatgcttgac cttattgcca cgactaagct caccaaagtc ttgactgagc
     2881 tggtagaaga gcgtgaggaa gagctgctaa ttggtgattt agagttaatt aaccaaattg
     2941 tgcgtgagca ttggctgaat attatcaaca aactgcgtga gcaacagaag aacactcaaa
     3001 aaggcgtgcc gagtgataag acaaaagtta tctacgagac cgcagaaaag ctgatcaaca
     3061 atatccgtaa tgttgcccat cagtttactg cacttgtgct tgcggtaatt aataaaaaac
     3121 cgcctttgac cgtgcgaatg gtaatggata gcggcacaat ccataaagta gctcacgcct
     3181 tttatgggga ttatgagcga gcggatgagc tattacgtct taatccgcat attcgccagc
     3241 caaactttat tgagcgaggc acgctgttaa attgttattg ggagtaaaaa tggaaaatga
     3301 aattgtggtg gagattgacg gctcgcagca taaaaactgg aaaagttatg atattgacag
     3361 cgatttttta attcctgctg atagttttaa ttttgagttg ggtaaatcta gcaatataga
     3421 ggttttacct aattattcgg gcaaaacggc aatagttaaa atcaatggca aaacggtgct
     3481 cactggcatt gtggatacca cacatcacac cattagcaaa agcgggcgtg ggtttagcct
     3541 aaatggacgt gataaagccg ctattttggt ggattgctct gccccgatta ccaatgtgca
     3601 agggctctct ttgcttgatg ctgttaaaaa aattgtaacc cctcttggga ttaaagaggt
     3661 gcaactgaaa gccgagaaaa acccgacttt aagcaaggtg gatattgatg tgggtgaatc
     3721 ggcgtgggag gcgattatgc ggtgtgccaa ctcggcaggt ttgcattgtt ggtttgaccc
     3781 caaaggcgtg ttaattgtcg gcggtgcgga ttacgctcaa ccgcccgttg ccacgttata
     3841 ttgcgtgaaa gagtcggggg atagccgtaa aaataacttt gttgatgcaa cactttctta
     3901 tgatgtgtcg caaagctatt cggaggtcac ttttcttgct caaaaacacg gcaaagattc
     3961 tgataatgcc aagcacgatt ttaaatgggt gtataaaaat gaggaaatgg agctttataa
     4021 accgaaaact atcgtgctgg gcgatgtgga gaacctcgaa gccttgatga aacaggcgaa
     4081 aaaacaggta tcagattggc agttggagtc atttaacctc acgattattg tacctgacca
     4141 taaaaccaaa gacggcacgt tgtggcaagc tgggcaacgt gtgcacgtgg tatgtgagga
     4201 gtatgattta gatggtattt tctttttaat ggggcgacgt tttcgactct ctcgcacggg
     4261 tggcacaacc accgaattgc ggttaaaact ggatggcgtg tggacacctg atgcttatca
     4321 agctaaagca caagaggctc gcaagcgtaa aggcaaaaaa ggacgtaaga aaaaagagcc
     4381 tgtagaatat gtaggctcgt gggagttggg aaaataatat gatgagaaaa ttagcaaaac
     4441 agactaaaga ggtggcaaaa ggtgtgcaag atagcgtgcg tgctgctttt agaggggtat
     4501 taaatttagt caagagtggc agtgatattc agcaggtaca ggtgtccgct ttagcggacg
     4561 aaactatcca agatttagag ctaatgcagc attttggttt cacttccgtg ccacctgccg
     4621 gcacgcaagc ggttgttatc cccttaggtg gtaaaaccac gcacggtatt attgtggcga
     4681 ctgaaaacgg ctcgtttagg gttaaaaatc taaaaaatgg tgagacggct atttatgatt
     4741 caagcggctc aactatcata ttaaaaaacg ggcgagtgat tgatattgag tgcgatagct
     4801 ttaacattaa atgcaagcaa tattccgtca ccgcaagcgg tggagcaaat tttaacaccc
     4861 cgaaactaga aaccgaccag cagtttgtgt ctcaagggca gatcagcggc aacggtggaa
     4921 tggtagttca aggcggaagt ggtgcaagtt tcactggctc attaacacaa caaggaggca
     4981 gtatctcaac tgatggtgat gtggtggcat cgggtaaatc actggttaca catcaacaca
     5041 acgaacaagg cgatggcaga cctacctctg ctccaattta acttacaagc taatttacaa
     5101 gcggtctaat tttgcaaatt ttttgtagaa aaagaccgct tgccatttgt ggaagtctct
     5161 ccccgttact cctgtcttta atgtctctac catagcaata tggacagaga aatcagcccg
     5221 ctcactcggg actatacaag tgaaaatata actacactgc aaaatgccgt gtatatcaga
     5281 ctaactactc cgaaaggctc gtggtgggca gatgggcgtg taggttctct gctccatctt
     5341 attcaacgtg agaaagattt aagccgtgtg gggctattag ctcaacagta cgccgaagag
     5401 gctttgcagc cgattattga tgacggacga gccaaaagca ttacggtgac acacgagcag
     5461 ccaaaagacg gctcgttaaa tcttttaatc tccgttattg ataatcgtgg ggttaaattt
     5521 gagtttaaac accctgtaaa actggtttag gtggttttta aaatgtatca aatcccaacg
     5581 cttgaagatg ttagaagaga aattctgcga gatattcaat ctttagagcc aactgctgat
     5641 attgaggtgg attcggatta ttttgttcgt gcctccgctt tggcaagctg tgtggtgggg
     5701 ctgtatgctc accaaaactg gattgttcgt cagtatttcc cagatacggc agactccgaa
     5761 tacctcgaaa tgcacgctaa attgcgaaat ctttaccgta aaaatgcgac ctatgcgagt
     5821 ggttcgttga aagtttttgg tactgctggc tcagtaatca atgagggctt gcaagttaaa
     5881 tttggcgagc gtttttatgt aaccaaaagc tcggacgtta ttggtgatga tggctctgca
     5941 acggtgcgag tgatttcaat ggcaacaggg gcatcaagca atgttaaatc tgaaagtaaa
     6001 gcacaactga ctgctgctcc tgctggcgta tcaagcgatt gcttgttatt gcctgatgtg
     6061 acaggcggga cggaagtgga aagcgatgcg gcattgctgg ctcggttatt agagcagatt
     6121 cgccgaccac cgtcaggcgg gaataaaaat gacttccgcc aatgggcaga aagcgttgat
     6181 ggcgtaacct ctgcgtttgt ttatccatta cggcgtggcg agggtacggt agatattgca
     6241 atcacctcag gtgataccgt gccaagtgat gaaatcgtgc gagcaactca agcttatatt
     6301 gatgaagtcc gccctgttac ggctaaatcg gtttatgtac ttaagccagt tgaaaaacgg
     6361 gtcaatttta acatcaaagt gcaacttgat acggatacga cgcttgatgg tgtaaaagcg
     6421 gaaatcgaaa acgctttaga gaattatttt ttatcactca aacctgctga caccttaatt
     6481 atttcgcaaa ttgaggcagt aattagtgat ttagtgggcg tagtagatcg tgagattgta
     6541 cagccaacca gaaaccaaac gattgatgcc taccgtgaaa tgggctggtt cagattgggt
     6601 gatgtaactg tggagttgat gtgatggtaa cgattaactc taaacacggc aaggcattgg
     6661 gcggtttatt accgcctgta tcgtatgacc caaacggcag atggctagcg ttatcccttg
     6721 aagttgaggg gagagagcta gaccgcattg atgcgagagc caagacttta atcgatgccg
     6781 ttgatgtatc aacaggcatt tttattgatg attgggagcg ggtttgtggc ttaacggcga
     6841 atagctcact accgcttgaa acccgaattg agcgtgtgat tgcaaagctg aatcagttgg
     6901 gtggattgtc gataggctat atcactcaag tggccaaaga gcttggatat tgcatcaaaa
     6961 ttagtgagcc acaacccttt attgctgggc taagccgagc tggggatatg ttatggcata
     7021 aagacattat gtggacattt tttgtggata tttgcaaccc cacagatgat tacaagcgtt
     7081 ttagagcaag tgtgtcaatg gctggcgatg cgttaatggg acgatacaaa gacccaattt
     7141 tagaaagtct acttgaagag gtaaaacctg catttagccg tgtgtgggta agatatttag
     7201 gagatattga atgaaacaag tgatgaatcc gattaatacc ccaacacagc gttttaaaga
     7261 tggcaaccct gccactggtg aatatggcac gattgtgact gctgaatttt tgaataacgt
     7321 ccaagattcg gtcattaaca cccaacaaga attgcacagt gtacttgctg aagcaggaat
     7381 cgaagcaaat gacgaacaag ttaatcaggt ggctaaagca atcaagaaaa ttgcgggcga
     7441 tgctactcgt gataatttta acgcattagc caaccctgat ggttacaaac acatcggtcg
     7501 ctgcaaatcg gttgcggagt tacgtactat
//
LOCUS       DANLXQ010000124         7520 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.124, whole genome shotgun sequence.
ACCESSION   DANLXQ010000124 DANLXQ010000000
VERSION     DANLXQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.124"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            109..705
                     /gene="nfuA"
                     /locus_tag="PXD94_001998"
     CDS             109..705
                     /gene="nfuA"
                     /locus_tag="PXD94_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL6286831.1"
                     /translation="MEEQTIHISISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAE
                     CGVSYCPPNAVEETDTEFKFNRFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNA
                     KMRKVADDAPFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSM
                     VDVTLKEGIEKQLLAMFPDELVGVKDITEHQHGEHSYY"
     gene            complement(873..2510)
                     /gene="pyrG"
                     /locus_tag="PXD94_001999"
     CDS             complement(873..2510)
                     /gene="pyrG"
                     /locus_tag="PXD94_001999"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL6286832.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYCQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
     gene            2774..3823
                     /gene="ansB"
                     /locus_tag="PXD94_002000"
     CDS             2774..3823
                     /gene="ansB"
                     /locus_tag="PXD94_002000"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL6286833.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGRGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQLALT
                     QTNDIKTIQQYFEDF"
     gene            complement(3897..4541)
                     /locus_tag="PXD94_002001"
     CDS             complement(3897..4541)
                     /locus_tag="PXD94_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286834.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTFDSFVK"
     gene            complement(4542..5525)
                     /gene="cysB"
                     /locus_tag="PXD94_002002"
     CDS             complement(4542..5525)
                     /gene="cysB"
                     /locus_tag="PXD94_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL6286835.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            complement(5522..6205)
                     /gene="yjjG"
                     /locus_tag="PXD94_002003"
     CDS             complement(5522..6205)
                     /gene="yjjG"
                     /locus_tag="PXD94_002003"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL6286836.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFVKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            complement(6281..7174)
                     /locus_tag="PXD94_002004"
     CDS             complement(6281..7174)
                     /locus_tag="PXD94_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL6286837.1"
                     /translation="MNKKLPPLNALKAFESAARHLNFTKAAEDLFVTQAAVSHQIKLL
                     EDFLGASLFHRRNRLLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQ
                     TFGMHWLVPRLADFHEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRL
                     TESRIVILASPEYLSQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGS
                     IFSHTFMVLQAAMHQQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSA
                     QVQKVQFFVEWIQQKMKECCA"
BASE COUNT         2297 a         1573 c         1356 g         2294 t
ORIGIN      
        1 taaattttat taacaatttg caaaaatttt tttagctagg caattttgaa attcaggcgt
       61 aaaatagttg ataaattttg taaaatatta aaaaacagga taatttaaat ggaagaacaa
      121 accattcata tttcaatatc agatgcagca caagcccatt ttgccagact attagaacag
      181 caagaagagg gaactaatat ccgtattttt gtggtcaatc ccggcacacc gggggcagaa
      241 tgcggtgtct cttactgccc acccaatgca gttgaagaga cggataccga atttaaattt
      301 aaccgtttct ctgcatttgt cgatgatatt agcttaccgt ttttagacga agcggaaatt
      361 gattatgtaa ccgacccaat gggttcacaa cttaccctca aagcccctaa cgccaaaatg
      421 cgtaaagtgg ctgatgatgc accgtttatc gagcgtttag attatgtgat tcaaacccaa
      481 gtcaatccac aacttgccag ccacggcggt aaagtgacct taattgaaat caccgaagat
      541 aaatatgccg tgctacaatt cggcggtggt tgtaacggtt gctcaatggt ggatgtgacc
      601 ttaaaagagg gaattgagaa acagcttctg gcaatgttcc ctgatgaatt agttggtgtg
      661 aaagatatta ccgaacacca acacggtgaa cattcttact attaaaaata ctaaaaggga
      721 taaatcattg atttatccct ttcttataat taatgagcaa gcggtcaaat ttactcgctt
      781 tcgctcgcac ttcgtgccat cagcaaagcc gatgttcaaa cgcaagcgtt tgtacaaaaa
      841 aatgagtaaa ttcgaccgct tgttaagttt gattaagcct tacgactctc ttggtgatct
      901 ttcgcagctt tcacaaaacc tgcaaataat ggatgaccat cacgcggagt tgaggtaaat
      961 tccggatgga attgtgctgc tataaaccaa gggtgatttg gcacttcaat aatttccact
     1021 aatttacgat ctgccgataa accgctcact tttaagcctg ccgcttcaat ttgtggtaac
     1081 agcttattgt ttacttcgta acggtggcga tgacgttcaa caatcgtttc cgcaccatat
     1141 aactcacgag cttttgagcc ttcaattaaa tggcattgtt gtgagcctaa acgcattgta
     1201 ccacctaaat ctgactcatc gctgcgggtt tccacattcc cttccgcatc ttgccattcg
     1261 gtaattaagc caatcaccgg ctgtgagcaa tctttatcaa attcagaaga atttgcctcg
     1321 gttaaacctg ccacattacg ggcatattca attagggcga tctgcatacc taaacagata
     1381 cctaagtaag gaatcttgtt ttcacgggca tattgtgcgg ttcgaatttt gccttccacg
     1441 ccacgatagc caaagccgcc cggcacaaga atgccgtcta agcctgccaa taactctgtg
     1501 cctttggttt caatatcttg tgaatcaatg tatttaatat tcactgttaa acgatgcgtt
     1561 aaacctgcgt gtttcaaagc ctcattcacc gatttatacg catcaggtaa ttccacatat
     1621 tttccgacca taccgatagt cacttcgccg gttgggtttg cttggcagta tagcacttgt
     1681 tcccactctg aaagatcggc ttctttgcaa tctaaacgga aacgatcgca cacaaaggaa
     1741 tctaaccctt gcgatttcaa taattccgga atgcgataaa tcgaatctac atctttaagc
     1801 gaaataaccg cacgctccgg cacattacag aaaagggcga tttttttctt ctcattttcc
     1861 ggaatagcac gatctgaacg gcaaattaac acatccggct gaataccgat tgaaagcaac
     1921 tcttttactg aatgctgagt cggcttagtt ttcacttccc ctgcggttgg aatataaggc
     1981 actaaggtta aatgcataaa caaggttttt tcacgaccga tatccaccgc taattggcgg
     2041 agtgcttcta agaatggcag tgattcaata tcccccaccg tgccacctac ttctacaatg
     2101 gcgacatcac gccctttacc gccttctaac acacgggctt taatctcgtt ggtaatatgc
     2161 ggaataacct gaatggttgc acctaaatag tcgccacggc gttctttacg caacacttca
     2221 gagtaaattt taccgctggt aaagttgttg gctttgctca tttttgaacg gataaaacgc
     2281 tcgtagtgac ctaaatccaa atcggtttcc gcaccgtctt cggtcacaaa tacctccccg
     2341 tgctgagttg gactcattgt acccggatcg acattgatat aaggatcaag cttcataatg
     2401 gttacattta aaccacgagc ttccagaata gatgctaaag aggctgccgc aatacccttc
     2461 cctaaagaag atacaacacc gcctgtgacg aaaatataat tcgttgtcat tgtgattgcc
     2521 tttgctgatt gattaggttg gaaattcaga atatcagttt acttcgtaaa aaagaaacta
     2581 tcaggacggg aaagtagttt acatcaaatt acattttttt gctaatggaa aatcggcaag
     2641 cagtcagatt aattcttttt tcaataatgc cgactaatct taattaatac actattattt
     2701 gcgatagatc agaattaacc tacatgttta tagatatcat aatacctgtt tttctaatac
     2761 tggagacgat acaatgaaaa tcaaaaaatt actactgcca actctgttaa gcattgctac
     2821 tatgccaatt caagcagccg acctacctaa tattacaatt cttgcaactg gagggacaat
     2881 tgccggtagc caaaaaaata cggcaacctc agcttatcaa gctgggcagc taaatattga
     2941 aacactcata gaagccgtcc cggatattaa aaaattagca aatatcaaag gagaacaaat
     3001 cgttaaaatc ggatctcagg atatgtctga tgaagtatgg ctaaaactgg caaaagcaat
     3061 taatcaacaa tgcccaaaca cagatggctt cgttattacg cacggcacag acactatgga
     3121 ggaaaccgta tacttccttg atatgactgt aaaatgcgaa aaacccgtag tattagtggg
     3181 ggccatgcga ccagcaactg aaaaaagtgc cgacggcgta ttaaatttat ataactcagt
     3241 tgttgtcgcc aaagattcta aatctagtgg cagaggggtg ttggttgcaa tgaatgatat
     3301 tgtattgggc gctcgcgatg ttacaaaaac cagtactaca tcagtgcaaa catttaactc
     3361 accaaacttt ggcgcactag gctatatcca taatagcaaa gttgattatg agcgctctcc
     3421 tgaaagtaaa cacaccgtaa acaccccttt caaagtagat gaattaacgg aattgccaaa
     3481 agtcggcatt atttatgctt attcaaatat gccaaccgaa ccgcttcaag cattattaga
     3541 tgcgagttat caaggcatcg tggttgcagg agttggcaat ggcaatatga atgcggctaa
     3601 tttggcttta cttgaagaag cagctaaaaa aggaattacc gtggttcgct cgtctcgagt
     3661 gccaaccgga tatactaccc gagatgcaga aattgatgat agcaagtatg gttttattgc
     3721 ttccggcaca ttaaatcctc aaaaagctcg agtattactg cagctcgcct taacacaaac
     3781 aaatgatata aaaactattc aacaatattt tgaagatttc taaaaagtaa ataacaagcg
     3841 gtctattttg caaaacaaat cacaaaatag accgcttgtc tcattactaa tctttattat
     3901 tttacaaagc tatcaaaagt tagctcataa ttgtcgccat cacgactata ggcgatatag
     3961 cgagggaatt tttcaccttc aaattttttc accgtaatgt cttttttatc actggttcta
     4021 aacttgtagg taatttcttg gtaggttttg ccgcctactt ttacctcttt ttgggctttg
     4081 cgtagctgca cattcggtgt tgggtaaagt tttttaccgt tagtagtttg aaaactgctc
     4141 ggtaatttat cgtaatagct caactgaaag gctgtggtga acaaatcata agttggcata
     4201 gtcagcgctt cttcttttag tggctctttc actttgccat attttaccgt tttagaatca
     4261 atttctgcca tcgcataagt tttgccgtta cgggtatctc ggtagctcaa catatgaaat
     4321 tgttcgtttt tttctgttcc cttcgcagaa aagacaatat tatataaagg cacattaatt
     4381 tttgcctgaa tattatattg gccaccaccg ttattaaagt taatgtaggc tggcattaaa
     4441 taatttgagc tgtatttcaa cttaaaattc tccgcactgg cgtttgaaat caagcccaaa
     4501 gtgaccacga ttacgctcgc taacttctgc caaattctca tctatttctc ctctaaagat
     4561 atactttcga acaaccttga aattgcattg ttatctcgta attgttccgc tttctccact
     4621 tgtacccgtg ttaaatgtgg cgaaaaataa ttaataaaat catacatata gttacgaaga
     4681 aaagttcctc gtttaaaggc gatttgcgtc atactcggtt gaaacaggtg acttgcattg
     4741 atagcgacta agtctttatc cgtttccgta tgtgccattg atgccataat accaacccct
     4801 aagcctaagc gtacataagt tttgattaca tcagcatctg tcgccgtaaa tacgatatta
     4861 ggcaaaatac cttccttatt gaaggcatga tctaaatccg attgccctgt aaaaccaaaa
     4921 gtataggtta tcaaatcata tctacctaat tgttcgacag tcaaattttt actttggttc
     4981 acaaaagccg caagaggatg ctctggtttg acaataactg aacgattcca ccaataacac
     5041 ggcaataaaa ccgcttcatc aaataaatat tgagcttctg tggtaatggc taaatccact
     5101 tctccagcca ccaaagcatc ataaatttga ttcggggagc cttggtgcaa atgcaaactt
     5161 acttcaggat agcgagcctt aaacttctca attacactcg gtagcatata acgggcttgg
     5221 gtattggtgg tcgcaatgcg taagatgcct ttgttgggtc gagtttgttc ttcagcaacc
     5281 gatttaatgc tctgggcttt taccaataat tcacgagcaa tcgccacaat tttttcacca
     5341 gcaggcgtta gccccttaac gtgcttaccg ttacgctcaa aaatattaat tcccaactca
     5401 ctttccaaca aacgaacttg tttgctaatc cccggttgag aagtgtaaag cacctctgcg
     5461 gcctcggtaa tattcaagtt ctggtttact atttcaacga tataacgtaa ctgttgtagc
     5521 ttcataatga tttcccctct acaatcgcaa taatctgttg catcgtatgc acttcgtaag
     5581 ttggcttaat atccgtatca ttctctttac taagcaaatt taaccaacaa gtatcaattc
     5641 cagcattatt acctcctaaa atatcagaag cgagggtatc gcctaccata agcactttag
     5701 tacgatcaaa ttcgtccatt aatgcaaaag cgtaatcaaa cacttggcga tcaggctttg
     5761 cggcaccaat ttgttctgat atcactacaa tatcaaaaaa atgtgaagtt tgagtattat
     5821 ctaaacgttt ttgctgcaaa tcggtaaaac cattggtgat aatgcccatt tttactttac
     5881 catataaagc atttaacata tccattacac cttctaaagg ctgactgacg aatgccatct
     5941 ccgccattaa ctcttgatta agttgtagcg gatctacgcc tgtttgagca gataatttta
     6001 caaaacgtct ggtttgaata tcttgagcag tgatctcatt attttggtaa gccacccaaa
     6061 gtggtttatt taccgcttga aagtcttgat aatcttgttc actaaaatca atttggtaac
     6121 gagccagcat cgcttttaaa ccaagataag aattaaatga aaacaaggtt tcatcagcat
     6181 caaataatac ccattgatat ttcataatat tcctttttta acttagataa caagcggtca
     6241 aatttttcta atttttcgca taccgctgat aaagccacgc ttaagcacaa cactctttca
     6301 ttttttgctg aatccactca acaaaaaatt gtactttctg aacttgggct gattgcgaag
     6361 gatagacaac atagaacgat ttttcatcaa acaatgtggt tgggaaaggc tctattaaag
     6421 tacctttttc aatttcgtgc tgtgccaaca actggttggc aatcgcaatg ccttgttggt
     6481 gcattgcagc ttgaagcacc ataaaagtat ggctaaaaat tgagccagtt tccgcatcta
     6541 ctatatcttc tatatgcaaa tgttcaacca ttcttttcca cttattatgg gtggaggaat
     6601 gtaataaatg cttacctgct aaatccgccg gtaaatgaat ggtattttgc gataaatact
     6661 caggcgaagc taaaataaca atacgggatt ccgtcaaacg gatagattcc agaccttgcc
     6721 aatccccctt accataataa atagcaacat ctgtttcttt acctaataat ccctcatctt
     6781 gatcaacacc cttaatacgt acttccactt ctggatattt ttcgtggaaa tccgccaaac
     6841 gtgggactaa ccaatgcata ccaaatgttt gtggtacgct aatagtcaga atttgacgac
     6901 tactttgctg cctaatttta tctgttgcga gagcaatttg ttctaataat ggctgaactt
     6961 catagaaata ttgtgcgcct aactcagtta attccagcaa acggttacgg cgatggaaca
     7021 gggaagcacc taaaaaatct tctaatagtt taatttggtg gctcactgca gcttgtgtga
     7081 cgaagagatc ttctgccgct ttagtaaaat ttaaatggcg tgcagcagat tcaaatgctt
     7141 tcaatgcatt gagtggtgga agttttttgt tcataatttt tcaatctaat agattactaa
     7201 acaagcaact ctattatttt ttctgatagt ttaaataaaa aaaattcgtt tgatgcttgc
     7261 aaatagaaat aataaaatat ccgcatactt agatggatag tttagaattt atttctattc
     7321 ctaaatttta agtatgatgt tgtgtttgca tattggtcta ggaaactaga ctagagtaac
     7381 gaaagttact tgtttcactt cctgtatatt ttgaaccgta ttggtttata accgcccact
     7441 ttgggcggtt tttttctatg taaaaaaaca gcacaaaacc tagatcgcat tctatattat
     7501 tcgctacctt tattctagcc
//
LOCUS       DANLXQ010000125         7445 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.125, whole genome shotgun sequence.
ACCESSION   DANLXQ010000125 DANLXQ010000000
VERSION     DANLXQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.125"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(4..798)
                     /locus_tag="PXD94_002005"
     CDS             complement(4..798)
                     /locus_tag="PXD94_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6286838.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVGPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWAS"
     gene            complement(899..1975)
                     /gene="fbaA"
                     /locus_tag="PXD94_002006"
     CDS             complement(899..1975)
                     /gene="fbaA"
                     /locus_tag="PXD94_002006"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL6286839.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            complement(2072..3247)
                     /locus_tag="PXD94_002007"
     CDS             complement(2072..3247)
                     /locus_tag="PXD94_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL6286840.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLATELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSSEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            complement(3502..4860)
                     /locus_tag="PXD94_002008"
     CDS             complement(3502..4860)
                     /locus_tag="PXD94_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL6286841.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGSLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(4930..5757)
                     /locus_tag="PXD94_002009"
     CDS             complement(4930..5757)
                     /locus_tag="PXD94_002009"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL6286842.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKARRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(5771..6859)
                     /gene="aroB"
                     /locus_tag="PXD94_002010"
     CDS             complement(5771..6859)
                     /gene="aroB"
                     /locus_tag="PXD94_002010"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL6286843.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(6883..7404)
                     /gene="aroK"
                     /locus_tag="PXD94_002011"
     CDS             complement(6883..7404)
                     /gene="aroK"
                     /locus_tag="PXD94_002011"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL6286844.1"
                     /translation="MAEKRNIFLIGPMGAGKSTIGRQLAQTLGMEFMDSDSVIEERAG
                     ADIDWIFDVEGEEGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIY
                     LETTVDKQFERTQRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKV
                     VANQIIDMIDNLQ"
BASE COUNT         2299 a         1664 c         1288 g         2194 t
ORIGIN      
        1 gatttagctt gcccaagcat aggaactaag gtgctttcca aaatgacttt caattaaacg
       61 tttcgtaatt tgatgttgcg gattcagtaa aacctctttc gtcaagccat actctactac
      121 ttcgccactt tgcatcacca ttattttatc ggcaatatgt ttgatcagac ctaaattttg
      181 ccccacataa atataagcaa tcccttgtcg ctgctggaca cttaacatta aattaattaa
      241 ttgtgttttg actgaaaaat ccagtgaatt aatcgtatca tccgcaataa taatttccgg
      301 ttctaaaatt aaagcacgag caaacgcaat acgctgcttt tgcccactgg aagcctctga
      361 aataggaacg agcgcttgct caggatacat tcctaccaat tttagcgtat taaaaatacg
      421 ctcatttctt tcctcttcat ttaaatctgt cgccaagcgg agcggagcat ctaaaatttg
      481 tcctatatta taattaggat caaaggcatc gttagggtcc tgaaacatca tacgaatatg
      541 cttagctcgg tatttataat cgccaaatga aagcggtgtt cctctaaaaa tcagttctcc
      601 agatgttggc ccaaccatac cagcaaccat tttagccaag gtagatttcc cagctccgtt
      661 ttcaccaata atcgctaaag tttccttgcg atttagggta aaagaaactt cttttaccgc
      721 ataaaaatct tgtttatgaa ataggctact acgttcaaca aaacgcttat tcagatgatt
      781 aacttcaagt aatgccataa taaattggtg cttggaaata aagtgatagt ttaccgcaaa
      841 aaataaataa aaaaagaccg cttatacaag cggcctgatt tatggaattt tttgcaaatt
      901 ataaaacgtt cacacagttt aagtcttcga aagattgctc taagcgttta gacatagact
      961 cttccatttt acgtaaccaa acacgtggat cgtagtattt tttgtttggt gcatcagggc
     1021 ctgtcgggtt acctaattga ccttgtaaat atgcttcgtt tgctttgtag aattgaagaa
     1081 taccgtccca cgcagcccat tgggtgtcag tgtcaatgtt cattttgatt gcaccgtagc
     1141 tgattgcttc acgaatctct tcacgtgaag aaccagaacc gccgtggaaa acgaagtcta
     1201 atgactttgc cggtaaacca cgctctttag atacatactc ttgtgaagca cctaagattg
     1261 atggttttaa ttttacatta cccggcttgt aaacaccgtg tacgttacca aatgctgccg
     1321 caatcgtgaa acgtgggctg attgggctta attggtcgta aacatataaa acttcagaag
     1381 gttgggtgta aagtttagat tcatctacat cagaattatc tacgccatct tcttcaccac
     1441 cggttacgcc gatttcgatt tcaagggtca taccgatttt gtccatacgg gctaaatatt
     1501 cacggcagat ttccatattt tcttcgattg gctcttcaga taaatcgatc atatgtgaag
     1561 agaataacgg acgaccggtt tctgcgaagt gtttttcgcc tgcttcaagt aagccatcga
     1621 tccaaggtaa taagtttttc gcagcgtggt cggtgtgaag aatcacaggc acaccatatt
     1681 cttctgctaa ttgatgaacg tgttttgcac ccgcaatcgc ccctaataca tctgcacgag
     1741 cgcctgaagc cggcttaatg cctttacccg catagaactg cgcaccacca tttgagaatt
     1801 gcacgattac cggtgatttt acacgagcgg ctgtttctaa tacggcatta actgaatctg
     1861 aacccacgca gtttactgcc ggaatagcga agttgtgttc tttagcataa gcgaaaactt
     1921 tttgtacgtc gtcgcctgtt actacacccg gttttacgat gtttaataat gacatttttt
     1981 tcttcctttg tatgaaatgt gcaaaagcac attgttaaaa tatacggcca aatcttgcaa
     2041 aattttcaca aaatttgacc gcttgtaact gttagccgtt agctcgtttt tctaagattt
     2101 ccactgccgg taatacttta ccttccacga attctaagaa cgcaccgcca ccggttgaaa
     2161 tgtaagaaat tttgtctttg ataccgaata aatcaatcgc agctaaggta tcgccaccac
     2221 cggcaattga gaatgcaccg tttgccgttg cttctgcaat cgcatttgaa atcacttctg
     2281 ttcctttacg gaagttaggg aactcaaaca cgccgaccgg accattccaa aggatggttt
     2341 ttgaattacg gataatttca gctaattgct ctgacgattt ttcaccaata tcgaaaattg
     2401 attcatcagc tcgtacttca ctcaccgctt tttccgttgc cggtgcagtt tcagagaatt
     2461 cagtacctac acgcacatca accggaacag gaatattggt tacttgtgct aaacgttttg
     2521 cttcagggat taaatcttct tcgtataatg atttacccac agcgtgacct tctgcggcaa
     2581 taaaggtatt ggcaatacca ccgcccacga ttaattggtc ggcaattttt gaaagcgaat
     2641 caagcacagt taatttagta gatactttag aaccacccac aatcgctaac attggacgtt
     2701 gtggctcttt taatgctttg cctaatgcat ctaattcagt ggctaataac ggacctgcac
     2761 acgcaacagg ggcatattct gccacaccgt aagttgaacc ttccgcacgg tgagctgtac
     2821 cgaatgcgtc cattacaaac acatcacaaa gtgatgcata tttttttgct aactcagggt
     2881 cgtttttctt ctcacctttg ttaatacgca cgttttcaag caccacgatt tcattctcgt
     2941 ttacttcaac accgtctaag taatcacgca ctaaacgaac aggaacacct aaatctgatg
     3001 catttaagta atcaactaca ggttgtaaag agttttcctc ttcaaatacg ccttctgtcg
     3061 gacgacctaa atgcgaagtt accattactt tagcaccttt ttgtaacgct aattttaagg
     3121 ttggaatggt agcaacaata cgggcatctg atgttacttt gccatctttt accggtacgt
     3181 taaggtcagc acgaataaat aaacgtttac ccgctaaatc aaggtcagtc attttaataa
     3241 cagacatagt ttgttcctct ttagattaaa atttaaaagt gataacggtc ggattatatc
     3301 agtttctaat cattttgggc taacctagat caaataaatg attaattttt tatcatttta
     3361 gaatgctatt atcctgactg ttcttattcc cccccaatat gcttggttaa ttaagcaaga
     3421 attgcaaatt ccccacaaaa tttaaccgct tgtcactaaa aataaagctg tttgaaaatc
     3481 cattcaaaca gcttatagaa tttagagtga attaaagaaa atcaccaaga taatcaccgg
     3541 aatcacaaag cgaacatagt taaaccaaat agtggtaaac attttacccg cacctaattc
     3601 ctccttcgcc tcttctttta gaacaaagcc aacgaaaatc gcgctaccta aggcggttaa
     3661 aataaacaga atattgccac taatataatc gaaggcatca aaaatgctct tgcccataac
     3721 ctgcacatct tgcaatatat tgtcacttaa tgctgatgga atattaccca acagaaaaat
     3781 agtagccaaa gtaataaaaa tagctttttg acggctaatc tttgccttct cttgcaatgc
     3841 agtgataata acttcataaa tagttaacga ggttgttaat gccgccacca ctaatagact
     3901 aaaaaagata accgcaaaaa ctgttccccc ccacatattt gagaacacaa tcggcaaact
     3961 ttggaatact aaagtcggac ctgaatttgg agcaacgcca aagctaaata atgacggaaa
     4021 aatcataaaa ccggcaagta ccgcaattaa ggtgttcatc accccagtaa ttgttgctgt
     4081 ttttactaaa ttctcgctct tatcaaggta gcttgaaagg gttatcaaca caccaaaacc
     4141 aaggcttaat gcaaaaaata cttgacccag cacaaacaca aacaattttg gtgtgatttt
     4201 tgaaaaatcg ggcatcagat aaaaggcaat gccttcagca gcccccggta aggtaatatt
     4261 tcgaaccacc atcacaatca ggaaaataaa cagaatcggc atcaaatatt tcactgaacg
     4321 ctcaataccg tccacaatgc cttttaccaa aataaaataa ttcaccaata caaaaagtgc
     4381 cgtataagca ataattgtca ttgggctatt aaaaatactt tgttggaaga attgttgggt
     4441 cgtgccaacg gtaacaggtt gagacagatc tagtgtcccc gtgagtaagc taccgatata
     4501 atttaatacc caaccaccaa gtaccatata gtaggcaaga ataccaaatg ctccaagcaa
     4561 gcccatatag ccaagaattt tccaacctga ggcaattttt ttaccattag caaaaccggc
     4621 aaaagcatca atagcattca ctctcattcg gcgaccaatt acattctcca ctaatatcat
     4681 agggatacca attacgatca tcgcaataca aaataaaaac acataagcac cgcctccgtg
     4741 ttcccccaca agataaggga aacgccaagt tgcaccaaaa ccgactgttg caccagcaac
     4801 ggtcataata tacgccaatc ggttagacca agattggcgt tcttgttgtt gatttgccat
     4861 ttaatactcc atcggctaac aataccgctt caaccaacaa gcggtcagat ttttagaaaa
     4921 tattacaaat taaatgaaga gtgcgaataa ctcgccaact ttaacccttg agcccacctt
     4981 ctgccctatt gaacgctgta cctgaatttg ggtaatatcg gcagattgat aaatcctacg
     5041 ggtaaaatca gtatcgtgat ttgaaattaa tacgggaacg cctaatcctt tcatttctaa
     5101 agccagtgtt gccaaacgcc cttgttgctc taggctaaaa ccaccgccag catattgcgt
     5161 aaaattcgtt tcttgtgcta aaggagcgta aggtgggtcg cagtaaatca cataatccgt
     5221 taattgattt tttgccaact caaagacctg ttcaaaatcc gcacacacaa aagttgcctt
     5281 ttgtgctttt tcagcaaaaa aacgtagttc tttttctgga aaataatggg ttttataacg
     5341 cccaaaggga acattgtacc cttttttctg gttataacgg caaagtccgt tatagccaaa
     5401 tcgatttaaa tacaaaaaaa tcaccgagcg acgaaaaata tcttttgagc gattaaactc
     5461 cgcacggcgt gctttataaa aggcttctgt attcgcttgt gggtgaagaa acagttgctt
     5521 tgtttgcgca atatattcct caacatctgc tttcaccacg ttgaaaagat taatcaagtc
     5581 aggattaata tcagcaagaa tataacgttc aaaatctgta ttcagaaaga ccgaacctgc
     5641 accaacaaaa ggctcgacta aacaagcctt ttttggcaaa tgggtttgaa tatctggaat
     5701 taagcgatat ttaccgccag cccattttaa aaaggctcga tgcttagggt gattcatttc
     5761 attttaaaca ttaacgttgg gtaaaacgct caatagaggc taatacttga gattctgtgg
     5821 cttgcgatga aacgtaagct tcaccaagtg atttcaataa aattaggcgt aattgacctg
     5881 ccagtacttt tttatctcgc cacatataag gtagatattc tgccggttcc attccatcag
     5941 gtgaaattgt tggcaaattg gctcgggcca ataatttttc caagcgagca acctcttgag
     6001 gacttaaatc ccccagaata cgagataatt ccgcagcctc tagcatacca accgaaacag
     6061 cctctccgtg taaccataca ccatagccca tacgtgcttc aatggcgtga ccaaaggtat
     6121 gtcctaagtt tagtaatgca cgatctcctt tttccgtttc atctcgagca accacgtcag
     6181 cttttagctg gcaacaacgc tgaatacaat attccagctc atcttgcttg agactgacca
     6241 aatcatcaat atgcttttca agccattcaa aaaaagtaac atcaaagatt gcaccgtatt
     6301 tgatgacttc cgccaaacca gcactgactt ctcttttgga taaggtatgg agcgtattgg
     6361 tatcaacaat cacagaaaca ggctgataaa aagccccaat catattttta ccgagtgggt
     6421 ggtttactgc cgttttcccg cctactgaag aatcaacttg agccagtaaa gtggtcggaa
     6481 tttgtataaa acgaatacca cgttggtaag aggcagctgc atagcctgca acatcaccga
     6541 ttacaccacc gcctaaagca attaaagtgg tatcgcgatt atgatttttt tctaataatg
     6601 cggtaaaaat caaatttaat gattctagcg ttttatattc ttcaccgtcg ggaatcaata
     6661 ccgaatcaac gtggcaaccc agctccttta acgcatttgt taccacacta agataatgtg
     6721 tcgccaccgt tggatttgag acaatcatca ccttatctcc agattttaat gggctataac
     6781 tagcaggctg atttaataac cctgcaccaa ttataatcgg gtaacgacgc tcttttaatt
     6841 ccacattgac ttgtaacata tcttagctcc tttcttacct ggctactgta aattatcaat
     6901 catatcaata atttgatttg ccactacttt agcgctctgc tcgtccgtat gaatagtaat
     6961 atctgcaatt tcttcataca atgggttacg gattttcgct aactcttcca acgttttacg
     7021 ggggtcttct gtttgcaata aagggcgttt tttatctcgc tgagtacgtt caaactgttt
     7081 atccactgtc gtttctaaat aaataacgat accacgagcc gataacacat tacggttatc
     7141 tttagataaa atcgaaccac cgccagttga aagcacgata ccataatttt gcgtcagttc
     7201 atttagaata cgttcttcac gcttacggaa accctcttca ccttcaacat cgaagatcca
     7261 atcaatatcc gcacctgctc gttcttcgat cacactgtcg gaatccataa attccatacc
     7321 taatgtttga gcaagctgac gaccaatcgt acttttacct gctcccatcg gcccaatcaa
     7381 aaaaatatta cgtttttcag ccattatttc ttcttcattt taaaatttac taaacatttg
     7441 atctg
//
LOCUS       DANLXQ010000126         7397 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.126, whole genome shotgun sequence.
ACCESSION   DANLXQ010000126 DANLXQ010000000
VERSION     DANLXQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.126"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(3..185)
                     /locus_tag="PXD94_002012"
                     /pseudo
     CDS             complement(3..185)
                     /locus_tag="PXD94_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(540..4436)
                     /gene="purL"
                     /locus_tag="PXD94_002013"
                     /gene_synonym="purI"
     CDS             complement(540..4436)
                     /gene="purL"
                     /locus_tag="PXD94_002013"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL6286845.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGRKALEEANINLGLALADDEIDYLVENFTQLGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPKFEQPWENPLSKPNRIASALDIMIDGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEIRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKEDLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGECGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADPHFDNKPIDLPMNVLLGKTPKMQRDVLSKTVKN
                     PPLATADIDLKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPELGITIPVGKDSMSMKTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQLGDK
                     PADVVNVARLKGFFNAMQTLVAQGKLLAYHDRSDGGLITTLAEMAFAGNCGISVEIDR
                     LVDDLAVLFNEELGAVIQVRTEELSEVQAVLNAENLANLTFDIGTAVESDSFEITRNG
                     TTVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFAAKKDPNNKGLSAFLTYDVNED
                     IAAPYINKGVKPSVAILREQGVNSHYEMAAVFDRAGFNAIDVHMSDLISGRRNLNDFN
                     AMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSKFFANPNTLALGVCNGCQMVSNL
                     AEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGMAGSHMPIAVSHGEGQVE
                     FKNPQQLANLQQQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMPH
                     PERVFRAVSNSWCPEDWTEGGAWMRLFRNARVALKY"
     gene            4698..4877
                     /locus_tag="PXD94_002014"
     CDS             4698..4877
                     /locus_tag="PXD94_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286846.1"
                     /translation="MFKEKGYDEFLAEKIRLGLEDMQSGNGLSLDESKARTKQLIERK
                     ARELANFEQENIIYG"
     gene            4870..5157
                     /locus_tag="PXD94_002015"
     CDS             4870..5157
                     /locus_tag="PXD94_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6286847.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYRIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(5283..7385)
                     /locus_tag="PXD94_002016"
     CDS             complement(5283..7385)
                     /locus_tag="PXD94_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL6286848.1"
                     /translation="MKSFKLLPVSAAVLSVLAANAVAQTASTDAVVLDTVTVTDNQGL
                     KVQSNVVTTKKKDESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDN
                     TYSDSQILYHQGRHMLDPALVKIVSVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNS
                     DKNFGAKLGTGFSTNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTN
                     RVPYSALDKTSFLAKLGATLGDHRFVLSHSSERNEGQRLVREEFDVAPNGTKVSPYNR
                     LTVERQAPANRRITVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETGNGYAGGV
                     DYATKTKIVTHGANVNFDSALHENVLLKYGVNYRHQEAKPHTKFNPLVKNQEKTDTGV
                     YVEAITAPVDKVTLTTGVRYDHFKFKAMDGKERSNGAFSPSVGLIYEPIEHLSLSASH
                     NYATRSPRLYDALTAHGNRKIVSIGSNTKAEKARNTEIGFNYNNGTFSFDGSYFWQDI
                     KDALGGGRDSHNPNNELIMNLGKIKNRGYELNAGYHNGGFTARIGVAHSKPRFYSPTI
                     WEVNRQTGKREEKPLLSTNPEYASAIGRTWTASLSYRFEQPNLEVGVHHRIVEKVKPE
                     DNFFVTGGTLQTNTGTGKDGYNVTDITLNWKPLKDDSVNVNFAVDNVANKKYNAHGQR
                     GQLSARGREFRAGVNYTF"
BASE COUNT         1960 a         1681 c         1554 g         2202 t
ORIGIN      
        1 tcctagaaac tcaatttaac caattgtttt aaaaagaaaa cttgatattt taagttactg
       61 tgattaaaac gccatctgca ttctttcaaa tagaattcat gtactttagg aacgccgtta
      121 aatttacgca aatagcattt tgcctgactc tccaaattct caattccgtt aatgtgattt
      181 tgattgctaa ctaaaaacca ctttctatta aggtagaatt gaccgttcaa ataaatcacg
      241 ttcattttct gtataagacg taatggggca tttaaaaggg tgaacagggt ccaataagat
      301 ttttcaataa gtttccctat ttacgtaagc ataaattgtg gggcaaccca cttttgtggc
      361 aagaggctac tgcgtagaca ccgtagatgt caatgcagaa atgataagaa agtacttgaa
      421 atatcaggaa aagcacgaag gataaacaac ttcgtttatc agatatataa aaattcgggt
      481 tctatgaatt cgaattttaa tcgcccccta tgggggcaga tcactacgaa ggtagatttt
      541 taatacttca acgccactct cgcatttcta aacaaccgca tccacgctcc accttccgtc
      601 caatcttctg ggcaccaaga gttactgacc gcacggaaca cacgttcagg gtgtggcatc
      661 ataatagcga ctcgtccgtc tgtattaccg atagcggtga tacccaacgc cgatccgttc
      721 gggttggctg ggtattgttc ggttgggttg aggtggctat ccacatattg tgcgatcacg
      781 agattttgct gttgcaagtt cgctaattgc tgtggatttt tgaactcaac ttgtccttca
      841 ccgtgtgaaa ccgcaatcgg catatggctg cctgccattc cgttgaacca aagtgagttg
      901 gtgtcgttga ttttcaccat tgcagcacgg gcttcaaaac gttcggattt attacgcacg
      961 aagcgtggcc agttttctgt gcctggaata atttccgcta gattggagac catttggcaa
     1021 ccattacata cccctaatgc cagcgtgttc gggttggcga agaatttgct gaattggtca
     1081 cgcagcattg ggttgaacag aatcgatttc gcccagccgc cgcctgcacc taagacatcg
     1141 ccgtaagaga agcctccgca cgccaccatt gcgttgaagt cgttgaggtt gcgtctgcct
     1201 gaaatcaagt ctgacatatg cacgtcaatg gcgttgaaac cggcacggtc aaagacggct
     1261 gccatttcgt agtggctgtt tacaccttgc tcacgcaaaa tcgccacact cggttttacg
     1321 cctttgttga tgtatggggc ggcgatgtct tcgttcacat cgtaagttaa aaatgcggat
     1381 aagcctttgt tattcgggtc ttttttcgct gcaaattctt ggtctgcaca ttcagggttg
     1441 tctcgcaaac gctgcatttg atgagtgagt tccgcccaaa tgccacgcag ttcggaacgt
     1501 ttttggctaa atactgttgt gccgttgcgg gtaatttcaa agctgtcgct ttcaaccgct
     1561 gtaccaatat caaaggtgag atttgcaaga ttttcggcat ttaacaccgc ttgcacctcg
     1621 ctcaattctt cggtgcgaac ttggattact gcccccagtt cttcgttgaa taacacggct
     1681 aaatcgtcca ccaagcggtc gatttccacc gaaattccgc aattccctgc aaacgccatt
     1741 tccgccagtg tggtgattaa accaccgtcc gagcggtcgt ggtaggcaag caattttcct
     1801 tgtgcgacaa gggtttgcat tgcgttgaag aagcctttta agcgagccac attcaccaca
     1861 tcggcaggtt tgtcgccaag ttgtttgtaa acttgagcaa gtgcggttgc acccaagcgg
     1921 ttgttgcctt cgcccaggtc gatcagcaac aagcgggtat cgcctttgtc ggtgcgaagt
     1981 tgtggggtaa cggttttacg cacatcttcc acacgagcga aagcggaaat cacgagcgaa
     2041 agcggagcag taacagattt ctgttcgccg ttttcctccc aagtggtttt catcgacatt
     2101 gagtctttgc ctaccggaat ggttatgcct aattcagggc aaagctcttc gcccaccgct
     2161 tttactgcct cgtaaagccc tgcgtcttcg ccctcgtgcc ctgccgccga catccaattc
     2221 gccgagagtt taatgcgttt gatgtcgcca atgttagtgg cggcaatatt ggtgatgctt
     2281 tctgccacgg cgaggcgagc tgaggcagca aagtcaagca aagcaacagg tgatcgctcg
     2341 ccaattgaca ttgcttcacc gtgatagctg tctaatgaag cggtggttac ggcacaatct
     2401 gccacaggaa tttgccacgg gccgaccatc tgatcttgtg ccaccattcc cgttactgag
     2461 cgatcgccga tggtaatcag gaaggttttt tcagccaccg caggcaagcg tagcacacgg
     2521 tggaacgcct ctttcaagtc gatgtcagcg gttgcaagcg gtggattttt cacagttttt
     2581 gacaaaacat ctcgctgcat ttttggcgtt ttgccgagca acacattcat cggcaaatca
     2641 atcggtttat tgtcaaaatg agggtcagca agggttaaat gcttctcttt cgttgcctcg
     2701 ccaatcaccg caaatggggc tctttccctt tcgcacaatg ctgtgaaaat gtcgagtttt
     2761 tcaggggaaa cggcaagcac ataacgctct tgcgattcgt tacaccaaat ttcaagtggc
     2821 gacattcctt tttcatcgca caagatgtta cgcaaatcaa acttaccgcc acattcgccg
     2881 tcgtgaacca attcaggcat cgcatttgat aaaccgccag cccccacgtc gtgaataaat
     2941 aaaattgggt tctccgcccc catctgccag caacggtcga tcacctcttg gcaacggcgt
     3001 tccatttcag ggttttcccg ctgaaccgag gcaaaatcca aatcttcttt cgatttcccc
     3061 gatgccattg acgatgccgc cccaccgcct aagccgatgt tcattgccgg gccaccaagc
     3121 acgatcaact tcgctcccac cgggatttcg cctttttgaa cgtgttcggc acgaatgttg
     3181 ccgataccgc ctgctaacat aatcggcttg tgatagccac gaatttcttc gcctgcgaag
     3241 ctgttcactt tttcttcgta agtacggaag tagcccaata acgcaggacg accaaattca
     3301 ttgttaaatg ccgctccgcc tagtgggcca tcaatcataa tatccagtgc ggaggcaatg
     3361 cgattcggct tggaaagcgg gttttcccac ggctgttcaa attttgggat cactaagtta
     3421 gagaccgaaa aacccgtcaa gcccgctttc ggtttcgccc cacgccctgt cgccccttca
     3481 tcacgaattt caccgcccga accggtcgcc gctcccggga atggcgagat ggcggttggg
     3541 tggttgtgag tttctacttt cattaaaata tgagcatctt cttgatgata gcgatattgt
     3601 ccatcttggt cggggaagaa acgcccaacc gttgagccct ccatcaccgc cgcattgtct
     3661 ttgtaggcgg aaagcacata gtccggggtt ttctcaaagg tgtttttaat catcttaaac
     3721 agggattttt cctgttttac cccatcaatc gtccaatccg cattaaaaat tttgtggcgg
     3781 caatgctccg agttggcttg ggcgaacata tagagttcaa tatcattggg gttacgccca
     3841 agttgggtaa agttttccac caagtaatca atttcatcgt ccgccaacgc taaacctaag
     3901 ttgatattcg cttcttccag tgccttgcga ccaccgccta aaatatccac cgaagtaaac
     3961 ggcttcggct cttgctgagc gaaaagctgt tcggcttggc tttcgttatc cagcaccgtt
     4021 tccatcatac ggtcgtgtaa taagcctttc aacgtcgtaa gttgactttc gttcggcgtt
     4081 tcagaaaatt ctaaataata tgccgtgcca cgctcaattc gctctactgc tgccaaaccg
     4141 cagttatggg cgatgtcggt tgccttagac gaccacgagg aaatcgtgcc gatacgaggg
     4201 gtaacaatca ggcaaaaccc tgtcggctcg tcttcttcca gcgttggacc gtagtgcaat
     4261 aactctttga ttttgttttc ttcatcggct acaagcggtc gatttaatgc cacaaaatgc
     4321 aaaaactccg cataaactga ttttacaggt aattgatttt gttggaattt ttgcagaagt
     4381 tgttgaatgc gaaaagcaga taaggcaggc gagccacgga aggtttggat tgtcattctt
     4441 tggattttcc tatttaagat gaaaaactaa accgattata aacgattgcg caaacgattg
     4501 ctatatttgt cagacgtttg ctgttagata catttattca ttagtatcaa ataaatttgt
     4561 tcaatactaa tgaatacctt tcaaagaagg tcccctctat caatctgttt taaaagataa
     4621 tttaaaaata agttaatttg acaactcaaa attaaaagca gacaatgctt ttaattatat
     4681 cttaatagga gcagattatg tttaaagaga aaggctatga tgaattccta gcagaaaaaa
     4741 ttcgccttgg cttagaagat atgcaatcag gtaatggtct ttcactcgat gaatcaaaag
     4801 cacgaaccaa acaactgatt gaacgtaaag caagagaact cgcaaacttt gaacaagaga
     4861 atatcattta tggctaaaac attacgaatt agcagtagag ctcaaaatga tattgaagaa
     4921 attttagcga gcgtaattga atatactggt tttgaaagta gtggaattcg cttacaagaa
     4981 gatatttacc aaaaatttga gactatcgct tatatgccct ctgcggctgg acgtttaaga
     5041 gaagatggca caagagaagc attcactagg cgttatcgta ttgtttatac cgaattagaa
     5101 aatgaagtgt ggattattac cattattcat agtagcagac tttatccacg catttaaata
     5161 acaaacgact aaaatagtcg gttttttatt tgcaaaaaat taataaaata tcaccgcttg
     5221 taagctccat aaaaaaaagc ccgattgtaa catcgggcgt tttagtctga atttaagtca
     5281 gattagaatg tgtagttcac acctgcacgg aattcacgtc cacgagctga taattgacca
     5341 cgctgaccgt gtgcgttgta ttttttattc gccacgttat ccactgcgaa gtttacattc
     5401 acgctatcat ctttaagtgg tttccagttt aaggtgatat cagttacatt gtagccgtct
     5461 ttgcctgtac cggtatttgt ttgtaatgtt ccgcccgtaa cgaagaaatt atcttcaggt
     5521 ttcactttct caacaatacg gtggtgtacg ccaacctcta aatttggttg ttcaaagcga
     5581 tagcttaaag aagctgtcca agtacgaccg atagctgagg cgtattctgg gttagtactt
     5641 aataatggtt tttcttcccg cttaccagtt tgtctgttta cttcccaaat agttggtgag
     5701 tagaaacgcg gtttgctgtg tgcaacacca atacgagcag taaaaccacc attatggtaa
     5761 cctgcattta actcataacc acggttttta attttaccta ggttcataat taactcatta
     5821 ttaggattat ggctatcacg accaccacct aatgcatctt ttatatcttg ccagaaatag
     5881 ctaccatcaa aactgaatgt accattgtta tagttgaaac cgatttcagt attacgagct
     5941 ttctccgctt tagtattgct accaatacta acgatttttc tatttccgtg agccgttagt
     6001 gcatcgtata aacgagggct acgagttgca tagttgtggc ttgcacttaa gcttaaatgt
     6061 tcaattggct catagataag cccaacgctt gggctgaatg caccattact acgctcttta
     6121 ccatccattg ctttgaattt gaagtggtcg taacgtacac cggtagttaa agttacttta
     6181 tctactggag cagtaattgc ttcaacatac acgccggtat cggttttctc ttggtttttc
     6241 acaagagggt taaatttagt gtgtggttta gcttcttggt ggcgatagtt cacaccgtat
     6301 tttaataata cattttcgtg aagggcagaa tcaaagttta cgtttgcacc gtgggttact
     6361 attttagttt tggttgcata atctacacca ccagcataac cattacctgt ttcattctca
     6421 gaccaacgac cttgtactaa ctgatacacg ttagctgttg cttcttgagc aaaacctaag
     6481 tttttaccag tccattctaa gttagttctt tgaaccgtaa ttctacggtt agctggagct
     6541 tggcgttcta cagttaagcg gttgtacgga gaaacctttg tgccgttagg tgctacatca
     6601 aattcttcac gcactaaacg ctgaccttca tttcgctcac ttgaatggct taatacaaaa
     6661 cggtgatctc ccaatgttgc acctaattta gctaaaaagc tggttttatc tagtgcacta
     6721 tacggaacac ggtttgtgcc gaattggttt acgtaatctt taccgccttt atagtcgcga
     6781 tctctcacaa gattacctga gactaaagca tcaaagattt cgcctttacc atataaggtt
     6841 gcattgaaat tgtgagcgtg gtttgtgctg aagccagtac ctaattttgc accgaagttt
     6901 ttatcgctgt tttttaataa atctaatgca tcaacggttt tagcaattac tgcaccatta
     6961 gtttgaccaa taccggctga agcactgcct gcaccttttt gtacagaaac aattttcacc
     7021 aatgccggat ctaacatatg acgaccttgg tgatacaaaa tttggctatc tgagtaagtg
     7081 ttatctactt ttacatcaat tgagttttga cccataccac gaatatagag atattgtgat
     7141 gtaccgttac caccacctaa gctaattgcc ggctcatctt ttaataaacc acgcaaatcg
     7201 gtttgggtgc tttcatcttt tttcttagtt gttaccacat tgctttgtac ttttaaacct
     7261 tgattatctg ttacggtaac agtatctaat actactgcat ctgttgaagc agtttgagcc
     7321 actgcattag cagcaagtac gctcaatacc gcagcagaaa cagggagcaa tttgaagctt
     7381 ttcattttat ttatatt
//
LOCUS       DANLXQ010000127         7352 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.127, whole genome shotgun sequence.
ACCESSION   DANLXQ010000127 DANLXQ010000000
VERSION     DANLXQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.127"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            92..2548
                     /gene="thrA"
                     /locus_tag="PXD94_002017"
     CDS             92..2548
                     /gene="thrA"
                     /locus_tag="PXD94_002017"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL6286849.1"
                     /translation="MRVLKFGGTSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKIT
                     NHLVAIVDKAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQ
                     QVAAEAAQTKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
                     LESSVDIAESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACL
                     KADVCEIWTDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPL
                     NIPCLIKNTGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAAR
                     VFSTMSKAGISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDI
                     IKDLSIVSVVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIE
                     AVKATHKSLFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDK
                     MLLNENGLNLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYAR
                     ALKEGFHVVTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLA
                     AGDEVEKFEGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARK
                     LLILVRETGLPLEFEDIEIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEG
                     KVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGAGN
                     SVTAAGIFADILRTLQGGVN"
     gene            2548..3495
                     /gene="thrB"
                     /locus_tag="PXD94_002018"
     CDS             2548..3495
                     /gene="thrB"
                     /locus_tag="PXD94_002018"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL6286850.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRNGSVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(3610..4986)
                     /gene="cysS"
                     /locus_tag="PXD94_002019"
     CDS             complement(3610..4986)
                     /gene="cysS"
                     /locus_tag="PXD94_002019"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL6286851.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     EHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLGL
                     LEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDTP
                     NGTIWRKA"
     gene            5142..5651
                     /locus_tag="PXD94_002020"
     CDS             5142..5651
                     /locus_tag="PXD94_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL6286852.1"
                     /translation="MITLHTNFGDIKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRV
                     IDGFMIQGGGMTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINV
                     ADNTFLDYRSKEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVED
                     VVIESVSVA"
     gene            complement(5743..6672)
                     /locus_tag="PXD94_002021"
     CDS             complement(5743..6672)
                     /locus_tag="PXD94_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL6286853.1"
                     /translation="MEIALLLADKILQLTLMVLLGYLIVKTKLLKSEHSYPISVIGLY
                     IISPAMLITAFQVDYTTEILNGLYLAFLISVLLNGLLILLGWLLKRALKLDNIEQATA
                     IYSNSGNLIIPIVASLFGNEWVIYSTAFIVVQNVLFWSHLRLLICGKGNVPFRKMIFN
                     INIIAIVIGMMLFLLHIKLPATLANTLSTLGHMLGPLAMLVAGMLIAAIPFKEIITDK
                     RIYLVAFLRLIFIPLILLAVIKLGGFASWIENGKTVVMITFLATISPSAATATQMAVA
                     YGQDAKKASAIYGVTTLLCVFTMPLIIWLYQFL"
     gene            complement(6675..>7352)
                     /gene="fdhD"
                     /locus_tag="PXD94_002022"
     CDS             complement(6675..>7352)
                     /gene="fdhD"
                     /locus_tag="PXD94_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL6286854.1"
                     /translation="MMATPANLEDFALGFSLAEGILNAPSELYGLDVIEQENGVEIQL
                     EISSRQFVALKDKRRSMAGRTGCGICGVEQLEQVNQSCKIVKKNNRLQNVNPLILEDC
                     LLQLENAQFLSKETGASHAAAFFTPDGKLLAIREDVGRHVALDKLIGWYAKADFPIGF
                     VFVTSRASFEMVQKCLAVGIEMLCAISATTEMAVNMARENQFTLLAFARKGKATIYAG
                     EERLALN"
BASE COUNT         2156 a         1578 c         1569 g         2049 t
ORIGIN      
        1 attggctgaa ataatgaaag aataaaccga aaacccgcta caagcgggtt tttttgtata
       61 aaaatttaca aataatttat caggagtaac tatgcgagtt cttaaatttg gaggcacttc
      121 tcttgccaac cctgaacgct ttatgcaagc ggcagagatt atcgagaaag ctcatttggc
      181 agatcaagct gccggcgtat tatccgcccc agccaaaatt accaatcatc ttgtcgctat
      241 tgtagataaa gcgattgccg gtgaatcatt tcaatctaac ctttctgaag ctaccgagat
      301 tttccacaac atcattaacg gcttatatgc tacaaataat cactttgcta aagaagcact
      361 gttagcctta gtggaagcgg aattaaacca aattcagcaa gtggctgcag aagcagctca
      421 aactaaatcg ttgtcagata aaattgctgc cactattcac tgccgtggtg aaaaactctc
      481 tattgcgatg atgcaagcgt ggttcgaggc aaaaggctat gaagtcactc gtatcgaccc
      541 tgttgaaaaa ttattggctc acggcagcta tttggaatct tcggtcgata ttgccgaatc
      601 caccaagcga gttgatgcag gttcaattcc aaagaaaaat atcgtgctaa tggctggttt
      661 taccgcaggt aatgagcaag gcgaactggt gttattaggg cgtaacggtt cagattattc
      721 tgccgcttgt ttggcagcgt gtttaaaagc cgatgtttgt gaaatctgga cggacgtaga
      781 tggcgtttac acctgcgacc ctcgtttggt gcctgatgca gtatgcttag aaacaatgag
      841 ctatcaagag gcaatggagc tttcctactt tggggcgaaa gtgatccacc ctcgcaccat
      901 cgggccatta gtgccgctca atattccttg tttaatcaaa aataccggca atccggacgg
      961 aaaaggcaca ttaattgatg gcaatgtggc aaacgataat ctcaaagtaa aagggattac
     1021 caaccttgat aacgtggcga tgttcaacgt ttcaggttca ggaatgcagg gaatggtggg
     1081 catggctgcc cgtgtgttct cgacgatgtc aaaagccggt atttcggtga ttttaattac
     1141 ccaatcatca tcagaatata gcatcagctt ctgcgtgccg gcaaaatcgg cagaaaaagc
     1201 gttagcggct ctaaatactg aatttgcaca agagttatct gaaaacttac ttgacccgat
     1261 tgatattatt aaagatttat ccattgtttc ggtagtgggt gatggtatgc gtaccgccaa
     1321 aggaattgcg gctcgcttct tctctgccct agcccaagcc aacatcagta ttgtggcaat
     1381 tgcacagggt tcatctgagc gttccatttc tgctgtcgtg ccgttaaaca aagcgattga
     1441 agcagtaaaa gcaactcaca aatcgctatt taacagtaaa cgtgtggtgg atatgttttt
     1501 agtgggtgta ggcggtgtgg gtagcgaact catcgagcaa gtcaaacaac aaaaggagtt
     1561 tttggcaaag aaggatattg agatccgtgt gtgtgcttta gctaattcag ataaaatgtt
     1621 actcaacgaa aacggcttaa atttagacaa ctggaaagaa gatttagaaa aagcaattca
     1681 gccgtcagac tttgatgtat tgctttcttt catcaaattc caccacgtgg taaacccagt
     1741 atttgtagat tgcacttcgg cacaatcggt ggcgaacctg tacgcacgag ccttaaaaga
     1801 gggcttccac gtggtaacac caaacaaaaa agccaataca tcaggttacg attactactt
     1861 agaaatgcgt gaaaacgccc gccaaagcca gcacaaattc ctctacgaaa ctaatgtagg
     1921 ggcaggttta ccggtaattg aaaacttaca aaacctatta gctgccggtg acgaagtaga
     1981 gaaatttgaa gggattttat ccggctcgct ctctttcatt ttcggcaaat tagaagaagg
     2041 tttaaccctt tcgcaagcaa ctttaatcgc caaagaaaaa ggctttaccg agccagatcc
     2101 acgagatgat ttatccggtg cagatgttgc tcgtaaactc ttaattcttg ttcgtgaaac
     2161 cggcttaccg cttgaatttg aagatattga aattgaaggc gtattgccaa aaggcttctc
     2221 ggaaggaatg agtcgtgatg agtttttaca agctctaccg tctttagacg aagaattcag
     2281 caagcgtgta gaagctgcca aggctgaagg caaagtgttg cgttatgtgg gctcgatcac
     2341 aggtgataaa tgccgagttg ccattgaagc cgtggacgaa aaccacccac tttacaaagt
     2401 taaagacggc gaaaatgccc ttgcttttct cacacgttac tacaacccta ttccattatt
     2461 attgcgtggg tatggtgcgg gcaatagcgt aaccgctgcc ggtatctttg cagatattct
     2521 gcgtacttta caaggaggag tgaactaatg gcgttacgaa tttatgcccc agcttcaagt
     2581 gcaaatatta gtgtgggttt tgactcatta ggcgctgcaa tctccccgat tgacggctct
     2641 ttgctcggcg atgtagttca aattgaagag agtgaacaac aatttgagct agaaagtgca
     2701 ggttattttg tacgtaaatt gcccaaagaa ccacaaaaaa acattgtcta tcaagcctat
     2761 gtgctattta gcgaacgctt gaaattacgt aacggcagtg taaaaaattt acgcttaaca
     2821 cttgaaaaaa atatgccgat tggctcaggg ctaggctcaa gtgcctgctc gattgttgca
     2881 gcattagtgg cactcaataa attccacgat gaaccgttct ccaaaatgga attactggaa
     2941 atgatgggcg aattggaagg cagaatttca ggctcaatcc actacgacaa cgttgcccct
     3001 tgctatttag gtggcctgca attaatgaca caatcgctcg gcaatatttg ccaacctctg
     3061 ccattctttg atgaatggta ttgggtgctt gcctacccgg gcgtggaagt atcaaccgcc
     3121 gaagcccgtg ctattttgcc gaaaagctat actcgtcaag atatgattca gcaagcccgc
     3181 tacttaggat ctttcgtcca tgcttgccat acacatcaag atatcttggc tgctacaatg
     3241 atgaaagacc taattgctga gccttaccgt gaagatttac tgccgaactt tccggctgtt
     3301 cgtcaaggct gtaaagactt aggtgcgtta gcggttggta tttcaggttc cggtccgaca
     3361 atgtttgcga tagcccctga tctcgaacac gctcaacgtt tagtgagcta tttagagaaa
     3421 gaatatctgc aaaacaacga aggctttatt catatctgta aagtcgataa tcaaggagca
     3481 agagagctga aatagccctc tcgaacaacc atatcactaa ataacttaaa ggactgactc
     3541 caataccact tggagcagcc ctttttgatt aattttttga aaagtctatc acttataaac
     3601 aatcgaaggc tatgccttac gccaaattgt gccgtttggg gtgtcttcga ggacaatatt
     3661 tatcgctttg agcttatctc gcacttcatc ggcaaccgcc cagtttttag ccgctttggc
     3721 ttcgttgcgt tgtttgatta aggcttcgat ttctgccgtt tcatcgtcat tcgcatcacc
     3781 ttttaaaaac gcatcggggt cttgttccag taagcctagc acgccggcta actcttttaa
     3841 acgaacggct aaaccgttgg cttttgccat atcttcggtt ttgagtttgt tcaactcacg
     3901 ggcaatttca aataacaccg ctaatgcccc cggggtgttg aaatcatcgt ccattgcggt
     3961 tttaaaggct tctacatagt gttctccgat ggcttcgccc gacacatcac aaccacgcag
     4021 agcggtatat aaacgctcaa gagccgaacg ggctaaatca aggttgtcaa tgctgtaatc
     4081 taataacgaa cggtaatgag cggttaagaa gaaataacgt aacgattcgg catcgtataa
     4141 gcctaacata tggcgaatgc tgaagaaatt gcccaaagat ttcgacattt tttctttatc
     4201 aatggtaagc attccagtgt gtaaccaata attgacataa tcgccaccgt gagcacagca
     4261 agattgtgca atttcgtttt cgtgatgcgg gaacattaaa tccgagccac cgccgtgaat
     4321 atcaaaatgt tcgcccaact ctttgctatt cattgccgaa cattcaatat gccagcccgg
     4381 tctgccattg ccccacgggc tttcccagct tggctcaccg gctttcgaca ttttccacaa
     4441 gacaaaatcc atcgggtttt tcttgatgga tttaatttca acccttgcac cggcttgtag
     4501 ttgctctaaa ttttggcggg ataatgcacc atatttctgg aacgattcca catcaaacat
     4561 cacatcgcca tcggctgcga cataagcgtg tccgttgtga atcagttttt ctaccatcgc
     4621 aataatttca ggaatatgct tggtggctcg tggctcaacg tccgggcgaa gaatgtttaa
     4681 cgcatcaaaa tctttgtgca tttcagcaat cattctatcc actaattcat cgcaagtttc
     4741 gttattttct aacgcacgtt tgatgatttt gtcgtccaca tcggtaatgt tacgcacata
     4801 gcgtaaattg tagcctgaat aacgtaaata acgggcaatc acatcgaaag agacaaaagt
     4861 gcgaccgtgc ccaaaatgac aaagatcgta aaccgtgacg ccacagacgt acattcccac
     4921 ctgatttggg ttaatcggtt taaattcttc tttttcacgt tttaaggtgt tataaatttt
     4981 gagcataaag tcctcttgtg taacattcag taacaaatgg ggctaagtat agcgaataag
     5041 cggtctgatt ttgagatttt tttgcaattt ggcgtaaact ttttaataat agcgagcgaa
     5101 atttattttc cattccattt ttatttataa aggaacaaag aatgattaca ttacatacca
     5161 atttcggtga cattaaaatc gaattaaact ttgaaaaagc cccgattacc gctaaaaact
     5221 ttgaagatta cgctaaagaa gggttttata acggcacgat tttccaccgt gtgattgacg
     5281 gctttatgat tcaaggtggc ggaatgacgg caggtatggc tgaaaaaccg acaaaagatc
     5341 cgattcaaaa cgaagcgaac aacggtttaa gcaacaagcg tggcacaatc gcaatggctc
     5401 gcacttctga tccgcattct gcttcgtcac agtttttcat taatgtggcg gataatactt
     5461 tcttagatta tcgctcaaaa gagatgttcg gcagaagtgt agtgcaagat tggggctatg
     5521 cagtatttgg cgaagtggtt gaaggtatgg acgtggtcga taaaatcaaa ggcgtaaaaa
     5581 ccggcaataa aggcttccac caagacgtgc cggttgaaga tgtggtgatt gagtctgtaa
     5641 gcgttgccta attccgcaaa ttagcctaca agcggttgat ttttcacaat tttttgcatt
     5701 tctcagcgtt gcaaaaaagt agcagaaatc gactgcttgt ttttatagaa attgatacag
     5761 ccaaataatc agcggcattg taaacacaca aagtaaagtg gttacgccat aaatcgcact
     5821 ggcttttttg gcatcttgcc cgtaagccac cgccatttgg gtcgccgttg cagcagaagg
     5881 gctgatggtg gctaagaatg taatcattac caccgttttt ccattctcta tccagctcgc
     5941 aaaaccgccg agtttaatca ccgctaacaa aatcagcgga ataaaaatca agcggagaaa
     6001 agccaccaag taaatgcgtt tatcggtaat aatctcttta aatggaattg ctgcaattaa
     6061 catccctgcc acaagcattg ctaaaggccc taacatatgc cctaaagttg agagcgtgtt
     6121 tgcaagcgta gcgggcagtt tgatatgtag caaaaacaac atcataccaa tcacaatcgc
     6181 aataatgttg atattaaaga tcatctttct aaacggcaca ttgcctttgc cacaaatcag
     6241 cagccgtaag tgagaccaaa acaacacatt ttgcaccaca ataaaagccg ttgaataaat
     6301 cacccattca ttgccgaaca gggaggcaac aatcggaata attaagttgc cggagttgga
     6361 ataaatagcg gtggcttgtt ctatgttgtc taatttcaac gctcttttga gcagccaacc
     6421 aagcagaatt aacaagccgt ttaataagac ggagattaaa aatgccagat acaagccgtt
     6481 tagtatttcg gtggtgtaat ctacttgaaa agcagtaatc agcattgccg ggctgataat
     6541 gtaaagtccg ataaccgaaa tcggataact gtgctccgat ttgagtaatt tagttttaac
     6601 gatgaggtag ccgagcagca ccatcagggt taattgtaga attttatcgg ctaataaaag
     6661 ggctatttcc attatcaatt aagagcgagt ctctcttctc ctgcataaat ggtcgctttg
     6721 cctttgcggg caaaagcaag taaggtaaat tgattttcac gagccatatt cactgccatt
     6781 tcagtggtcg ctgagatggc acacaacatt tcaatgccaa cggctaagca tttctgcacc
     6841 atttcaaagc tggcacgact ggttacaaac acaaatccaa tcggaaaatc agctttagca
     6901 taccaaccga ttaatttatc caacgccacg tgtctgccca catcttcacg aatggcaagc
     6961 agttttccat ccggggtaaa aaatgcagcg gcgtggcttg caccggtttc tttcgataag
     7021 aattgagcat tttctagctg taataaacaa tcctctaaaa tcagtggatt gacgttctgc
     7081 aagcggttat ttttcttaac aattttgcaa ctttgattaa cctgctctaa ttgctctaca
     7141 ccgcaaatac cgcaacctgt tctgcctgcc attgagcggc gtttatcttt taatgcaaca
     7201 aattggcggc tcgatatttc aagctgaatt tctactccat tttcttgttc aattacatct
     7261 aaaccatata attccgatgg ggcatttaaa atcccttccg ccaatgagaa tcctaaagcg
     7321 aagtcctcca aattagccgg tgttgccatc at
//
LOCUS       DANLXQ010000128         7301 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.128, whole genome shotgun sequence.
ACCESSION   DANLXQ010000128 DANLXQ010000000
VERSION     DANLXQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.128"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            60..479
                     /locus_tag="PXD94_002023"
     CDS             60..479
                     /locus_tag="PXD94_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL6286855.1"
                     /translation="MVPSNEAVLKAVHDTLVISTTDLQGVITYANDLFCKLTGFTREE
                     LIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQPMFDE
                     TGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
     gene            550..2106
                     /locus_tag="PXD94_002024"
     CDS             550..2106
                     /locus_tag="PXD94_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL6286856.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEVLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGTLTQLFTAFSRDQAEKFYVQDA
                     LSQQAELVWHLIQQGAYFYICGSKAMSKAIDAELIKIADQIGGDPYIDDFNNIIAKLV
                     AEGRLMRDVY"
     gene            complement(2164..2421)
                     /gene="rpsQ"
                     /locus_tag="PXD94_002025"
     CDS             complement(2164..2421)
                     /gene="rpsQ"
                     /locus_tag="PXD94_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL6286857.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(2421..2612)
                     /gene="rpmC"
                     /locus_tag="PXD94_002026"
     CDS             complement(2421..2612)
                     /gene="rpmC"
                     /locus_tag="PXD94_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL6286858.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(2612..3022)
                     /gene="rplP"
                     /locus_tag="PXD94_002027"
     CDS             complement(2612..3022)
                     /gene="rplP"
                     /locus_tag="PXD94_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL6286859.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(3036..3743)
                     /gene="rpsC"
                     /locus_tag="PXD94_002028"
     CDS             complement(3036..3743)
                     /gene="rpsC"
                     /locus_tag="PXD94_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL6286860.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(3763..4095)
                     /gene="rplV"
                     /locus_tag="PXD94_002029"
     CDS             complement(3763..4095)
                     /gene="rplV"
                     /locus_tag="PXD94_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL6286861.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(4106..4381)
                     /gene="rpsS"
                     /locus_tag="PXD94_002030"
     CDS             complement(4106..4381)
                     /gene="rpsS"
                     /locus_tag="PXD94_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL6286862.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(4412..5233)
                     /gene="rplB"
                     /locus_tag="PXD94_002031"
     CDS             complement(4412..5233)
                     /gene="rplB"
                     /locus_tag="PXD94_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL6286863.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(5252..5557)
                     /gene="rplW"
                     /locus_tag="PXD94_002032"
     CDS             complement(5252..5557)
                     /gene="rplW"
                     /locus_tag="PXD94_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL6286864.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVGRRSDWKKAYVTLQEGQSLDFVEGAAE"
     gene            complement(5554..6156)
                     /gene="rplD"
                     /locus_tag="PXD94_002033"
     CDS             complement(5554..6156)
                     /gene="rplD"
                     /locus_tag="PXD94_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL6286865.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGSVTFAAKPQDHSQKVNKRMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(6172..6801)
                     /gene="rplC"
                     /locus_tag="PXD94_002034"
     CDS             complement(6172..6801)
                     /gene="rplC"
                     /locus_tag="PXD94_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL6286866.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(6827..7138)
                     /gene="rpsJ"
                     /locus_tag="PXD94_002035"
     CDS             complement(6827..7138)
                     /gene="rpsJ"
                     /locus_tag="PXD94_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL6286867.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
BASE COUNT         2130 a         1554 c         1357 g         2260 t
ORIGIN      
        1 actgataaac acataacaaa attcatttat attaatggag attcaacacg gctgaacaaa
       61 ttgttccttc aaatgaagct gttttaaaag cagtccacga tactttagtt atttcaacaa
      121 ctgatttaca aggtgttatt acttacgcta acgacctttt ctgcaaatta accggtttta
      181 cccgtgaaga attaattgga caaccgcata atatcgtacg ccacccagct gtaccaaaag
      241 aagtttataa agatatgtgg gatacaatcc aagccggtaa aatctggaca ggtatcgtac
      301 caaactgcgg taaaggcggt gtagtttatg tggttgatac caccgttcaa ccaatgtttg
      361 atgaaacagg caaaattaca ggctatatca gcgttcgccg tgtaattaat gatttaatga
      421 ccaactttga agcagtagaa tcagcaaaag aaatctttga tgacttctac ggtgaataat
      481 taaaaaacaa tgattgcgtg taaaagatga tctcttttac acgcaatttt ttattttcga
      541 ggagtttgta tgtctctcat tccccatatc cacattgctt atggttcgga gtctggtaac
      601 gcagaaaagc ttgcccaaca attagctatc caaccctttt tacttcccta ctcacccaca
      661 ctttctacat taaacgaaac cgatttaagc caattatctg ccgagagttt attgcttatt
      721 gtaaccagct cctttggtga tggcgaacca cctgaaaacg ccgatgaatt tgcagaaaaa
      781 ttggaaaatt tgaccgcttg taatctgcaa tatgcgatat tcgggctagg tgatattact
      841 tatgataaat tctgtggcta tagcaaacaa ctagattcat tattacaaca aaaacaagct
      901 aaagctgtga ttgcaagggt tgatgccgat ttaaactatc aagaaatttt taaacaatgg
      961 ctacccctag tggagcaggc attacattca acaaacaaag aaacgatcca acattccctt
     1021 tctgttcaag cttacggcga ggaaatggca taccaagcag aagtgttgga aattaagcat
     1081 ttggcaaact caagccctgc ggtgtatcac attcgtttat cattaaaaga gagcggtatt
     1141 ttctatcaag caggggattt aatttatgtc aaagtgcagc aaccagacag actattaaat
     1201 gaatatgcag aatggtttaa cgatcaaaat gcagttgcat tactacgcca taaagaactc
     1261 cgactactaa gtaaaaatgt actgcgtgat attcaaaaaa tttgcggcag taatgaactc
     1321 aaagatttaa ctaaaatttc caataaaaaa gcattggaac aatatgtcta tgggcgggat
     1381 ttattagacg tattacgcga ttttgaccca aacaaaagcg tgacattagc cgatttagag
     1441 ccaattttat ctaacttagc cgcacgagcc tattcaatta gctcttgcgg taaaactcac
     1501 cctgattatg ttgatttatg tatccgccac gtttcgtatc atttaaacgg tagagattat
     1561 caagggactg caagtgggta tttatccgaa ttacaagcgg gcgattttgt ctcgattttt
     1621 gcaaatgcca atccgaattt ccgtttaccc gaaacattaa ccgctccggt ggtgatgatt
     1681 ggctcaggta ctggcattgc tccgcatatc gcatttttac aagcgttaga gcagcaatat
     1741 cagggggttg aaagctattt attcttcggc gagcgttatc gtgataaaga ttttctgtat
     1801 caaacggaac tggaaaatta cttagcaaaa ggcaccttaa ctcagctgtt tactgctttt
     1861 tcacgtgatc aggcagagaa gttttatgtg caagatgccc tatcgcaaca agcagagtta
     1921 gtttggcatt taattcagca aggggcttat ttctatattt gtggtagcaa agcaatgagt
     1981 aaagcaatag atgcggaact catcaaaatt gcagatcaaa ttggaggcga tccttatatt
     2041 gatgatttca ataatattat tgcaaaattg gtcgcagaag gccgattaat gcgagatgta
     2101 tattagcaaa tgaaaaaaac taaaaaccca gcaaaagctg ggtttttatt taatcatagt
     2161 tcattaagca acaactgctt tctcaactac acgaactaaa gtccaagatt tagtttttga
     2221 aataggacga cattccttaa tttcaacaac atcacctaat ttagcttcat tgttttcatc
     2281 gtgtacatga agtttagtag tacgacggat aaacttaccg tataacggat gtttaactgt
     2341 acgttcgata gcaacaacaa atgatttatc cattttgtcg ctaaccactt taccttgtac
     2401 tgtacgaatt ttatcagtca ttattcaccc gccttttcgg ttaatacagt tttaacttgt
     2461 gcaatactac gacgcacttg ttttaactgg tgggtttgtt gaagctgacc ggtggctgct
     2521 tgcatacgca atttgaattg ttcacctaaa aggttcacta actcaccatt cagctcttca
     2581 acacttttat tacgtagttc ttgagctttc attacatcac cgtcttagtt acaaatgtgg
     2641 ttttgaacgg aagtttagct gccgctaatg caaacgcatt gcgtgcaact tcttcagaaa
     2701 caccatccat ttcatagaga actttacccg gttggattaa ggctacccag tactcaacgt
     2761 tacctttacc tttacccata cggacttcta atggtttttc agtaattggt ttgtctggga
     2821 acacacggat ccagatttta ccttgacgtt taaccgcacg gctcatcgca cgacgagcag
     2881 cttcaatttg acgagctgtt aaacgaccac ggccaatagc ttttaaacca aatgaaccga
     2941 agctcacttc tgtgccaccc gcgataccac ggttacggcc tttgtgtact ttacggaatt
     3001 ttgtgcgttt tggttgtaac attcagtgat tctccttatt tacgaccttt gccacgtggc
     3061 gcctttttag gctgtgccgc tggttctttt tcagattcaa tcactgcagc cattccacca
     3121 agaatttcac ctttgaagat ccacactttt acgccgatta cgccgtaagt agtgtgagct
     3181 tcagcagtac tataatcgat gtctgcacga agcgtatgta gaggtacacg accttcacga
     3241 taccattctg agcgagcaat ttctgcacca cctaaacgac cgctaacttc aactttgata
     3301 cctttagcac ctaatttcat cgcgttttga actgcttttt tcattgcacg acggaacatc
     3361 acacgacgtt caagttgaga agcgatgcta tctgcgacta atttcgcatc tagctctggt
     3421 tttttcactt cagcgatgtt gatttgagct ggaacgccag cgattttcgc aactgcgttg
     3481 cgtaattttt caacatcttc acctttttta ccgataacga tacccgggcg agctgtgtga
     3541 atagttacac gaatgctttt tgcaggacgc tcaatagtga tgcgtgatac agaagcgttc
     3601 gctaactctt tgtttaaaaa tttacgtact ttgaagtcgc cgtctaagtt atcagcgaaa
     3661 tcttgtgtat tcgcgaacca agtagagttc caaggtttaa caatacctag gcgaatacca
     3721 ttaggatgta ctttctgacc catttgttat tctcctactt aattaacgat ctgacacaac
     3781 cacagtgatg tggcttgtac gttttaaaat acgatctgca cgaccttttg cacgtggcat
     3841 tacacgtttc atgcttggac cttcatcaac gaagatttta gcaactttaa gatcatcgac
     3901 atctgcaccg tcattatgct ctgcgtttgc aatagctgac tctaatactt tcttaacaag
     3961 cgtcgctgct tttttgttag tgaaagttaa gatttctaat gctacagaaa cttttttacc
     4021 gcgaattaaa tcagcaacta agcgagcttt ttgggcagaa gtacgagcgt aacgatgttt
     4081 agcaatagtt tccatctttt acctcttatt tcttagcttt cttatctgcg gcgtgaccgc
     4141 ggtatgtacg agtcggtgca aattcaccta atttatgacc gatcatttcg tcggaaacat
     4201 aaacaggaac gtgctgacga ccattatgga ctgcgatggt caaaccaatc attgatggaa
     4261 tgatcattga acgacgggac caagttttaa ttggtttttt atccccgctt tccaccgcct
     4321 tctctacctt cttcaacaag tgtaggtcaa ggaaaggacc tttcttgagg gaacgtggca
     4381 ttggtgttta ccttttagtt aatgaattaa attatttgcc acgacgacgt acgatgaatt
     4441 tatcagtacg tttgttgtga cgagttttct tacctttggt ttgaacgccc caaggtgtaa
     4501 caggatgttt accgaagtta cgaccttcac caccaccgtg tgggtgatct accgggttca
     4561 ttgcagtacc acgaacagtt ggacgaatac ctctccaacg gtttgcacct gctttaccaa
     4621 gaacgcgaag catatgctct gagttaccta cttcaccaat ggttgcagta cactcagcta
     4681 atactttacg catttcgcct gaacgaagac gtaaagttac atagttacct tcacgggcaa
     4741 tgatttgaac gtaagcacca gcagaacgtg caatttgacc acctttacca ggttttaatt
     4801 caacgttgtg tacagttgta cctactggga tgttacgcat tggtaatgcg ttacctactt
     4861 taatcggagc tgaagcaccc gcttgaattt gatcaccagc agttaaacct tttggtgcta
     4921 aaatatagcg acgttcacca tctttataaa gtactaatgc gatattcgca gaacgatttg
     4981 gatcgtattc taaacgctca acaacacccg gaatgtctaa tttattacgt ttaaaatcaa
     5041 ttaaacggta gtgttgtttg tgaccgccac caatgtgacg agttgtgata cgacctaagt
     5101 tgttacgacc accagtttta gatttagtat ctaaaagtgc tgcaaaaggt ttacctttgt
     5161 gaagctcagc gtttacaact ttaacaacgt gacgacgacc agcggaggtc ggcttacatt
     5221 taacgatagc catttatttt atctcctctg attactctgc cgcaccttca acgaagtcaa
     5281 gcgattgacc ttcttgaaga gttacataag cttttttcca gtcactacga cgaccaactt
     5341 tagcaccacg gcgtttagtt ttacctttaa ctactacggt acgaacagaa tcaactttta
     5401 cttcaaaaag ttcttcaact gcatttgcaa tctcaacttt gtttgcatct aatgcaactt
     5461 tgaatacgat tgtattgcct ttttctgcgt tgtttgtcgc tttttcagag atatgtggtg
     5521 ctttaagcac gtttagcaaa cgttcttgtt gaatcatgct aacatctcct caatttgttt
     5581 tactgcatct acggttacaa ccactttatc gaaagcaatt aagctaactg ggtcaatacc
     5641 ttgcacatca cgcacatcaa ctttatataa gttgcgtgct gctaagaata aattctcatc
     5701 tacgtttgca gtaatgataa gagcgtcatt taacgccata tcttttaatt tttgtgctaa
     5761 tactttagtt tttggtgcat cgatttcgaa tttctcaaca accactaaac ggtcttgacg
     5821 aactaattca gaaagaatgc ttttgattgc accacggtac attcttttgt tcactttttg
     5881 gctgtgatct tgtggtttcg cagcaaaagt tacactaccc gaacgccaaa ttggtgattt
     5941 aatatcacca gaacgtgcac ggcctgtacc tttttgacgc caaggttttt tacctgaacc
     6001 agacacttca gcacgagttt tttgagcacg agatccttga cgagcacctg ctgcataagc
     6061 aacaactact tggtggatta atgcttcatt gaactcacgt ccgaaggtag tttcagaaac
     6121 agttagtgcg tttgcaccta caacttgtaa ttccatctct atctcctaga cttatgcttt
     6181 aactgccggt tttacgataa catcaccatt agttgcacca ggaacggcac ctttaactaa
     6241 taataattta cgctcaacat ctacacgaac aacttcaagt gattgaacgg ttacacgctc
     6301 tgcacctaag tgtcctgcca tttttttacc tttaaacaca cgacctggag tttggttttg
     6361 accaatagaa ccaagtacac gatgtgataa agagttaccg tgagttgcat cttgagtacg
     6421 gaagttccaa cgtttaacac caccttggaa gcctttacct tttgatgtac cagtaacatc
     6481 gactttttta acatcagtaa agatgtcaac attgatttct tgacctaaag tgaattcttc
     6541 accttcagta gtacgaaatt cccataaacc gcgtccagct tcaacacctg ctttcacgaa
     6601 atgacctgct tctggtttag ttacacggct agctttttta gagccagtag taacttgaat
     6661 tgcagagtag ccatcgtttt caagggtttt aacttgagta acacggttgg cttcgatttc
     6721 gataacggta actggaatag acacgccgtc ttcagtgaaa acgcgggtca taccaacttt
     6781 acgaccgatt aaaccaatca ttgtaataac ctcttaaaat tcccaattaa cctagactaa
     6841 tctgaacgtc aacgccagca gctagatcta aacgcattaa tgcatcaaca gttttttctg
     6901 ttggttcaac aatatcaact aaacgtttgt gagtacggat ttcatattgg tcacgtgcat
     6961 ctttattcac gtgtggagaa atcaatacag tgaaacgttc tttacgagtt ggtaaaggaa
     7021 ttggaccacg aacttgtgca ccagtacgtt tagctgtttc tacgatctcc gcagtagatt
     7081 gatcaattaa acgatgatca aatgctttta agcggatacg gattctttgg ttctgcatta
     7141 gaccagagct ccaattaaaa aatttagcta ataaaaaaac caaactacca cttaacaaat
     7201 tagttaaggg agctcagtta aacctatata gttccaaaat taggaacatt gtatacacta
     7261 caatatctgt agtgccattt gataaatgat tacatcaaat g
//
LOCUS       DANLXQ010000129         7286 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.129, whole genome shotgun sequence.
ACCESSION   DANLXQ010000129 DANLXQ010000000
VERSION     DANLXQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.129"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..506
                     /locus_tag="PXD94_002036"
     CDS             <1..506
                     /locus_tag="PXD94_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL6286868.1"
                     /translation="FIAQIKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIAEIEKT
                     VELLLNQTEVLYSEFYAEKLVKQVDALKNAVEKIQSKPESAQFHSSYQFSPNIHRLAP
                     NKRLQEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLKAIEDL
                     EQELLFK"
     gene            complement(510..583)
                     /locus_tag="PXD94_002037"
     tRNA            complement(510..583)
                     /locus_tag="PXD94_002037"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(549..551),aa:Cys,seq:gca)
     gene            complement(599..674)
                     /locus_tag="PXD94_002038"
     tRNA            complement(599..674)
                     /locus_tag="PXD94_002038"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Gly,seq:gcc)
     gene            complement(884..2308)
                     /gene="lpdA"
                     /locus_tag="PXD94_002039"
     CDS             complement(884..2308)
                     /gene="lpdA"
                     /locus_tag="PXD94_002039"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL6286869.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKKR"
     gene            complement(2508..4418)
                     /gene="aceF"
                     /locus_tag="PXD94_002040"
     CDS             complement(2508..4418)
                     /gene="aceF"
                     /locus_tag="PXD94_002040"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL6286870.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSTPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRR
                     LAREFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGL
                     LPWPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQ
                     NKEAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGVAVDT
                     PNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTH
                     FTPIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYI
                     NSVLADIRRLVM"
     gene            complement(4622..7279)
                     /gene="aceE"
                     /locus_tag="PXD94_002041"
     CDS             complement(4622..7279)
                     /gene="aceE"
                     /locus_tag="PXD94_002041"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL6286871.1"
                     /translation="MSENLRNDVDPIETQDWLASLDSLIREEGLERAQFIIEQVISQA
                     RAGGVALPTGVTTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLD
                     LGGHISTFQSAATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAE
                     QMDNFRQEAFTDGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKD
                     TSAQKVYAFLGDGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQ
                     ELEALFTGCGWEVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVR
                     EHFFGRYPETAALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKG
                     YRIPEAESKNTAHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLH
                     GQRKALNGYVPVRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKN
                     IGQQIVPIIADEARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEG
                     INELGATASWVAAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGG
                     TSGRTTLNGEGLQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQED
                     IFYYITTLNEITEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAA
                     AQILANDYGITADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVA
                     STDYMKMYAEQIRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLA
                     KEGTVENKVVAEAITKFGLNVDRINPLYA"
BASE COUNT         2054 a         1661 c         1373 g         2198 t
ORIGIN      
        1 tttttatcgc ccaaataaaa caaaacctct cggaattaaa tgtattttca acggataata
       61 tttttctgaa ttcaccctat ttctcacagc aaaccggttt ggtgagtgtg ttcattgctg
      121 aaattgaaaa aacagttgag ctattattga accaaactga ggtgttatat agtgaatttt
      181 acgctgaaaa attggttaaa caagttgatg cattaaagaa tgccgtagaa aaaatccaaa
      241 gtaagccaga atcagctcaa ttccattctt cctatcaatt ttctccaaat attcatcgtt
      301 tagcgcccaa caaacgctta caagaatacc gtaaagcgtt acgtgcattg aacgagaaaa
      361 ttagctggtt agttgagcaa aatttaaata cgcaaaatga agcaactaaa caaaccctgc
      421 aaaatcaaat tacagagaca gaatatcgca aaatgaaatg tttaaaagca attgaagatt
      481 tagaacaaga gttgctattt aaatagtgat ggaggcgtgt cccggagtcg aaccgagcta
      541 catggatttg caatccagtg cataaccgct ttgctaacac gccataagat ttggaaattg
      601 gagcgggaaa cgaggctcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac
      661 tgagctattc ccgcatctga attcccacaa cattgcgctg tggaacgagg tgcattttac
      721 ggatttatca ttttatgtca atgctaaatt taacattcta gattcaaatg ctgaaaaaaa
      781 actcactatt caatagttta gttttaaaaa taatcaagcg gttgtatttt gcaaaaaatt
      841 tacaaaatac aaccgcttat tttttagaga ttaaataatc tgattaacgc tttttggctt
      901 tcgcatttgg taagtctgta attgaacctt caaatacttc tgccgctaat cctaccgatt
      961 cgtgtaacgt tgggtgagca tgaatggtta atgcgatatc ttcagcatca cagcccattt
     1021 cgattgcaag accgatttcg cctaataatt cgccaccatt gctaccaaca atcgcaccac
     1081 ctaatacacg gtgagtatct ttatcaaaaa ttaacttggt cataccttct gagcattcag
     1141 aagcaatcgc acgacctgaa gcagcccaag ggaatttcgc cacttcgtag ttcaagcctt
     1201 cttgtttaca ctctttctca gttttaccaa cccaagccac ttctggttca gtataagcga
     1261 tggacggaat gactttcgga tcgaagtaat gtttcattcc tgcaatcact tctgctgcca
     1321 cgtgtccctc gtgtacacct ttgtgagcta acatcggctg gccgacaata tcaccaatag
     1381 caaagatgtg tggcacattg gtacgcattt gtttatctac acgaataaag ccacgctcat
     1441 ctacttccac tccagcttta ccggcatcaa ttaatttacc gtttggcaca cgtccgatag
     1501 cgactaatac cgcatcataa cgtttagtat cgttgcaagc cttaccttcc attgatacat
     1561 agataccgtc atctttagct tctactgcgg ttactttggt ttcaagcatt agcttgaatt
     1621 ttttctcgat ttgtttggtg taaatcgcta caacatcttt atctgcagca ggaattactt
     1681 ggtcgaacat ttcaaccact tccacttctg aaccaagcgc gttatatacc gttcccattt
     1741 ctaaaccgat aataccacca cccataataa ggagtttttt cggtacttct tttaatttaa
     1801 gagcatcggt tgaatcccaa actcgtgggt cttcgtgtgg aataaatggt agttgaatcg
     1861 gacgagaacc tgctgcgata atcgcattat cgaacgtgat agttgtagga ttgccgtcac
     1921 gatcacgagc gactaacgta ttcggaccag taaatgcagc caagccttct actactgtca
     1981 ctttacgagc cttagccatt cctgctaagc caccggttaa ttttgaaaca acggcttctt
     2041 taccagcacg tacctgatct aaatcaatcg ttggctcacc aaaggtcaca ccgttgtgta
     2101 ctgcattttt agcttcttca attacttttg caacgtgtaa taatgcttta gaagggatac
     2161 aacctacgtt taagcaaata ccacctaagg ttgaataacg ctcaacaatt actgtttcta
     2221 agcctaagtc ggcacaacgg aatgccgctg agtaacctgc tggacctgca ccaagtacga
     2281 caacttgggt tttaatttct ttgctcatag tttttcctct ttttattccc cccctttagc
     2341 aaagaggggt aagggagatt tggcagaata taaagcgaag tgagagagca atcaatcaaa
     2401 cacaaatttc taccaaataa tttattctct tcgtaaatta cgtctatcaa atctccccct
     2461 acccctcttt tctaaagagg gactttttta ggaggagctt taccgaatta catcactaaa
     2521 cgacgaatat ctgccaacac gctattaata tagcttaaga atctcgcacc atcagcacca
     2581 tcgatcacac ggtggtcgaa tgataatgaa agcggaagca ttaagcgtgg ttcaaactct
     2641 ttgccattcc aaatcggttg catttcagat ttagacacac ctaaaatagc cacttccggt
     2701 gcattcacga ttggcgtaaa gtgcgttgta ccaataccgc ctaaacttga aattgtgaaa
     2761 cagccacctt gcatatcaga tgctgtcaat ttaccttcac gggctttttt cgagacttcc
     2821 attaattcgc gagaaagttc gataatgcct tttttgttca catttttgaa caccggcacc
     2881 actaaaccgt ttggcgtatc aaccgcaacc ccgatattga tgtatttttt caaggttaaa
     2941 cgttgtgcat cttcagaaat tgagctgttg aaacgaggga aggcttctaa tgcacttgcc
     3001 actgctttca taatgaagac cacaggggta attttcacgt ctaatttacg tttttccgct
     3061 tctttatttt gctctttacg gaagttttct aactcggtaa tgtctgtgcg atcgaaatgt
     3121 gtaacgtgag gaatcattac ccagttacgg tgtaagttcg caccggaaat cttattgata
     3181 cggcttaatt caacttcttc aacttcacca aacttgctga aatctacttt cggccacggt
     3241 aataaaccta agcctgcacc gtttgccgca cctgttgctg ccgctgctgt tccgccttgt
     3301 ttttcaaata cttgaacagc cgttttaacg tatgcttgaa tatcttcttt aacgatacgg
     3361 cctttacgac ctgtaccttt tactttgtct aggtttacac caaactcacg agctaaacgg
     3421 cgaattaccg gtgttgcgtg tgcatagcca gctgaagcca ccacttgttc ttggcttaaa
     3481 cctgattggt tttgacctgc cggagctgcg tcagctttcg ccggagctgc tgcttgtgga
     3541 gcaggagcag gagctgaagc cgcttgagca gccgccggag ccggtgccgc acccgctact
     3601 tcaaatttca taattaatga accggttgaa actttatcac ctgatttcac taaaatttct
     3661 ttcaccacac cggcaaatgg agccggaact tccattgaag ctttatcacc ttcaaccgtg
     3721 attaaagatt gttcttctga aacagaatca cccactttaa ccataatttc agttacgttt
     3781 acttcatcac caccgatgtc cggtacattt acatctttaa tcgctgatgc cgaagcttga
     3841 gctggagcag cttccgcaac cggtgtagat tgtgctgtag ctgcggaagc cgtttcaaat
     3901 ttcataatca atttaccagt tgaaactttg tcacccacat tgattaagat ttcttttact
     3961 acgccggcaa ccggtgcagg cacttccatt gaggctttat caccttcaac gttgatgata
     4021 gattgatcca cttcaacgct atcacctact ttaaccataa tttcggttac gttaacttca
     4081 tcgccaccga tatcaggtac atttacttca acaacggctg aagttgttgc tactggagca
     4141 gcaggagctg cttctgcttt tggtgcttct tgagcaggag cagcatctgc tgactctaaa
     4201 attaacattg gtgaaccggt tgaaacttta tcacccactt tcactaaaac ttcttttacc
     4261 acacctgctt caggagccgg cacttccata gaggctttat cgccttctac gttaataatt
     4321 gattgatcta ctgaaatagt atcaccaact tttaccatca cttcagtcac actgacttca
     4381 tccgaaccga tgtccggcac attaatttgt tttgacattt tgaaatcctt cttgagttac
     4441 cccctctccc attgtgggag ggacagattt ttctttgtaa gaagaaaaat cagggagaag
     4501 ggaatgcaag cggttgattt tctcaacatt tttgcaaata aaactaatac cctctctgct
     4561 aaaagtttct aaacgaaacc tttagctgtc tctctcccat caagggagag agtattagaa
     4621 attacgcata taatgggtta atacgatcta catttaagcc gaatttagta atagcttccg
     4681 caaccacttt gttttcaact gtaccttctt tcgctaattg agcgagggct gcaactacta
     4741 cataacgctc atcaacttcg aagtgttcac gtaagtttgc acggctgtct gaacgaccga
     4801 aaccgtctgt acctaacacg tgatagtgtt tacttggtac ataagcacgg atttgctctg
     4861 cgtacatttt catatagtcg gttgaagcaa cagtcggtaa atcttgtaac acttgagcaa
     4921 cgtacggtac acgtggtgtt tcagttgggt gtaataagtt ccaacgagcc acatctgctc
     4981 catcacgacc taattcattg aatgatggtg ctgagaatac atctgctgta atgccgtagt
     5041 cattcgcgag aatttgtgca gctgcacgca cgtgacgcat aattgcacct gagcttaata
     5101 attgtacgtg acctttacct ttaccctcaa cggtttcaaa tttatataaa cctttacgga
     5161 taccttcttc cgcacctgcc ggcattgccg gctgttcggt aatttcattt aaggtagtaa
     5221 tgtagtagaa gatatcttct tggttttcac catacatacg acgaataccg tcttgaacaa
     5281 taaccgcaac ctcaaaggca aatgcagggt cataagtgac gcagttcggg attacacctg
     5341 cttgaatatg gctatgacca tcttcgtgtt gtaaaccttc accgtttaat gttgtacgac
     5401 ctgaagtacc accaatcata aagccacgag ctaattggtc accggctaac cacatcatat
     5461 cgcctacacg ttggaaacca aacattgagt agtagatgaa gaatgggatc atcggtaagt
     5521 tgctaacaga ataagaagtc gctgctgcca cccaagaggc tgttgcacct aactcattga
     5581 taccttcttg taacacttgg ccatcttttg cttcacggta gtaagctact aaatcacggt
     5641 ctgacggtac atagttttga ccatgtgggt tataaatacc gatttgacgg aataaacctt
     5701 ccataccgaa agtacgggct tcgtccgcaa taataggtac gatttgctga ccgatatttt
     5761 tatctttcaa taaagtattc aatacacgag agaacgccat tgtggttgaa ataccacgtg
     5821 gttgctcatc caataatgct tggaattcgc ttaattcagg cactttgaaa tcaaccgtaa
     5881 atttcggttt acgtacaggc acataaccgt ttaaggcttt acgttgtccg tgtaagtagt
     5941 tgtactcttc agaaccttca gggaatctta catactctag gctttctact tgctcatcgg
     6001 ttaatggtag ctcgaagtag tcacggaagc cttttaagct gtctaatgac atttttttcg
     6061 attggtgagc agtattttta ctttccgctt caggaatacg gtagcctttc acttggtgag
     6121 ctaaaattac aactggtttg tccgattttt gtgcttttgc ataggctgca taaagttttt
     6181 cagaatcgtg tgcaccacgg cgtaatgccc aaatttcatc gtctgtcata tcagcaacta
     6241 atgctgcagt ttcagggtaa cgaccgaaga agtgttcacg aacgtaagca ccatttttgg
     6301 atttgaaggt taaatagtca ccatcaagca cttccatcat taactgttta agtttgcctg
     6361 aagtatcttt ttcaagcagt ttatcccaac cactacccca taacacttta atcacttccc
     6421 agccacaacc ggtaaagagt gcttctaatt cttgcacgat tttgccgtta ccgttaaccg
     6481 gaccgtctaa acgttgtaag ttacagctaa tagtgaagat taagttattt aatttttcac
     6541 gaccggcgaa tgataatgca cctttagatt cgatttcatc catttcgcca tcgcctaaga
     6601 atgcatatac tttttgagcc gaagtatctt ttagaccacg gttttctaaa tattttaaga
     6661 aacgagcttg gtaaatagca tttaccggtc ctaaacccat agatacggta gagaattgcc
     6721 aaaattcagg cattaattta gggtgtgggt aagaagataa accgtctgta aatgcttctt
     6781 gacggaagtt atccatttgt tctgcggtaa tacgaccttc taagaacgca cgaccataca
     6841 tacccggtgc tgcgtggcct tggaagaaca ctaaatcacc accatctttt tcggttggtg
     6901 ctttgaaaaa gtggttaaaa cacacttcat acatagttgc cgcagattgg aaagttgaaa
     6961 tatgaccacc taagtcaaga tctttcttct ggctacgaag tactgccgca atcgcattcc
     7021 aacgtaccgc agaacgaata cgacgttcga ttttatgatc acccggataa gctggctgct
     7081 cggaaacagg gatagtattg acataatctg ttgttacgcc tgttggtaat gccacgccac
     7141 ctgcacgagc ttggctaata acttgttcaa tgataaattg agcgcgctct aaaccttctt
     7201 cacgaattaa tgaatcgagt gatgctaacc aatcctgagt ttcgattgga tctacgtcat
     7261 ttcttaagtt ctctgacata gtattc
//
LOCUS       DANLXQ010000130         7167 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.130, whole genome shotgun sequence.
ACCESSION   DANLXQ010000130 DANLXQ010000000
VERSION     DANLXQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.130"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            84..2383
                     /locus_tag="PXD94_002042"
                     /pseudo
     CDS             84..2383
                     /locus_tag="PXD94_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(2461..3162)
                     /locus_tag="PXD94_002043"
     CDS             complement(2461..3162)
                     /locus_tag="PXD94_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL6286872.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(3167..4576)
                     /locus_tag="PXD94_002044"
     CDS             complement(3167..4576)
                     /locus_tag="PXD94_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL6286873.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(4578..5309)
                     /locus_tag="PXD94_002045"
     CDS             complement(4578..5309)
                     /locus_tag="PXD94_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL6286874.1"
                     /translation="MNVNFYVTCIADVMKAGVAKNTVLLLEKLGCKITFLEKQGCCGQ
                     PAINSGYTKQALAGMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAER
                     AKKVADRFYDLTDFIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVK
                     GLELLPIKNQETCCGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNI
                     GGRLSREGKPIKVMHIAEVLMQGGK"
     gene            complement(5453..7057)
                     /locus_tag="PXD94_002046"
     CDS             complement(5453..7057)
                     /locus_tag="PXD94_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL6286875.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
BASE COUNT         2201 a         1562 c         1347 g         2057 t
ORIGIN      
        1 aaatattgca cttcttacaa aatatctgta aatttcattg ccattatttt tttatggcta
       61 tttattctag aaggactcta aacgtgttaa agaaaaatta tctcactgta tcaattctcc
      121 ttgccattag tggagtaggc tacgctaatg aaatctcttt agagactatt actgttgatg
      181 gaaatacgcc ttcaaccaaa gggaaattac taggtggtga actaaatagc aatgaatctg
      241 ttgttgatga aaaaaattaa aacaaggtag tattacatta ggtaacgctt tatctggtga
      301 attaggcatt cattcaagcc agttcggagg cggtgcaagt accccaatta ttcgtgggca
      361 agagagtaaa cgtgcaaaaa tcttacaaaa taatggcgaa aacttagata tgtcgggaat
      421 gtctcctgat catgcagtaa ccgtagatgc gctacttgct aaacgtattg aaattttacg
      481 tggtccaact actttacttt acagtgcagg taatacggca ggtgtaatta atgtggtaga
      541 caataaaatt ccaaccgcta ttcccgaaaa aggctatgag gggcaatttg gtgtgcgttt
      601 tggctcagcc agtaaagagc gtttaaccta tgcaggctca acctttgccc ttggaaatca
      661 tttagccttg cgggtacagg gaatgtataa caaagcttcc gaatactatg ctccacactt
      721 tacgattgaa ggcaaacctt accaccgcgt accagatagc gatgtacaat cacaaaccgg
      781 cacagtaagc ctttcttgga ttggagagcg aggacatctt ggtattgctt acactgatcg
      841 ccgtgataaa tatggattaa ttggtcacac acacaaatac gatcactata ctattagtat
      901 tattcgccaa gccgttatgt ttgcaaaagg ttatttacgt ttttatccgc accttgcaga
      961 agaaggagat attgactata acaaccctgg tatccgttta ctgcatactc atattccggg
     1021 aggctcacat tatggacaag atactcacga gcacggcaaa ccttggattg atatgcactc
     1081 caagcggtat gatattgatg gaagtttgca aaatccactt cctggttttg aagaagccaa
     1141 aatatcagct aactatgtag attactatca tgatgaaaaa gatggaaaaa gagttgaaaa
     1201 ttacttcaag aacaaaggaa aaaatctacg tttcgaattg gttcacaaag agtggaaagg
     1261 gctaaaagga gctattggtg tgcaatatac caaccaatct acctcagcac ttgcacttga
     1321 agcttcacga gctgcaaaag tatttaataa acagccttta ctcaataatc caaaaacaaa
     1381 attatggagt ctctttgcga ttgaacgcct taatttaggt gactttacct ttgagttaag
     1441 tgggcgggca gaacgtcaaa aaatagcaat ggattatgat gtgaaattaa ttgatcgctg
     1501 gctaggcttt aatacaccta tgccaaactt ggacccacat aaagacaaag gttattctta
     1561 tagctttgca actcattggt atttcgcacc aaaccataag ttaacattaa atgctgcaca
     1621 tcaagaacgt ttacctaatg ctcaggaact ctatgctcat ggtaaacaca ttgctttaaa
     1681 tgcatttgag gctggcaata aaaatctaaa aaaagagcgt tctaaccaaa ttgagctatc
     1741 attagcttat gttggcgaca aatgggatta taaattaaat ctctaccaca cccgctatgg
     1801 caactatatt tacccattaa ctcttaatga taatcgtgga cctaaatctt ttaccgatga
     1861 atataacttg aaagtgaata gatattatca aggcgaagct cgttttagtg gggcagaagg
     1921 cgaaattggt tatctattta caccaaatta tcgcttagcc gtattcggag actatgtccg
     1981 tggaaagtta gtcaacttac ctaatattgc aatgagttac aacatttgga caggtgaagt
     2041 tgacaaatgg gcttctcagc cagacatttc tgcacctcgt ataccaccac tacgtttagg
     2101 agcaagattt aatgctgact tcaacctaaa ctggtctgga atgttagaat attaccgagt
     2161 atttgctcag aaaaaagtct cgaaatacga acaagttacc cctggccatc atcaagtaaa
     2221 ccttggcgta acctatagta atcattttaa tcaaacagaa taccaagtat tccttaaagt
     2281 ggataacttg cttaatcaga aaatgtacca acatgcctca tatctaccac atattccaca
     2341 aatgggtaga aatgcaatgc ttggaatgaa tatcagtttt taatttgtaa atctctacaa
     2401 aaatataacc gcttgtaaat ttctatctac aagcggttac tttcagtgga ttttttgcaa
     2461 ttagagatcg tcaattacca agtaaatttt atttaccggg ccgtgaacgc caactacttt
     2521 aatcaactca atatccgcag tcgccgatgg gcctgagatg atattcacac aagatggcat
     2581 tctttcacct cgttgggctt tttcgtgcag aatttgtgca agctgtgcca cacgaggtaa
     2641 cactgtactt ttgcgtaaca caaccaccga tttttcgggc aataagctca ctgaacgccc
     2701 aaattcttta tcggaaaaca gtacaatacc gccagactct gccaagccat attcaccgta
     2761 aaccacaccg atattagcag ttctggcttt ttcaaagttg gtttcagctt ggttgaaatc
     2821 ccacacatag ctgtcttcgt attttgcttg aatggctgcg gtaatcccaa gatcctttaa
     2881 acgttgatcg ttattcacga tcacactcgc cccgccatat ttttcacaaa tatcaataat
     2941 ggcttgtggt aaatccgcct cttttgcctc tactacatct gccatcatca ctcgggcaaa
     3001 atcgataaac tcttggcaaa gttgtggttg agggcgatcg gttaaacggg taatcgcgtg
     3061 gttgttttcc aacacaggaa ttggctcagg cacattttgg cgtggtctgc ccatacgctg
     3121 tgccaattta tttaagaaat tttcacgatt ttgtaaatcc attttcttat cctctgttat
     3181 taaaccattc acggaaactt tcgccttcgg cagtcggtaa atcacgggct ttcgtccatt
     3241 cacctaacgc cccaaaattc accggtgctt tgccgttttt aatcaatttg cttgccactt
     3301 tcgcccccac ttttacgccg actttccaaa gggttgggtg tgagttagcg aagttaaagc
     3361 caaaaataga caagcgttct gccattggcg ttaagccggc atccgcaatt ttctcacggt
     3421 gttttaagat aagctgtgcc agtggaattt tcactggaca aacactgtta caagcggtac
     3481 aaagtgagca agcatacggc aattctttaa actcttcgta gccaccgagc aatggcgaaa
     3541 taactgaacc aatcgggccc ggatagattg aaccatagcc gtgaccgcca atttggcgat
     3601 acgccgggca agtgttcaaa catgcaccac agcggataca acgcaacact tcttggaatt
     3661 cgctttctaa gattttagaa cgaccgttat cgacaatgac caagtggaac tcttccggac
     3721 catcggtttc gccttcaagt ctaggacctg tcagccaagt gttgtaagcg gtgagttttg
     3781 cccctaccgc actgcgagcc agcatggtaa ttaatacatc aacctcttta aaggttgggg
     3841 cgatacgttc catacccatt actgcaatat gcgttttcgg cacagttgtt gccatacgca
     3901 gattaccttc gttggtcact aaacaaaccg agccggtttc cggcaccgca aagttacagc
     3961 ctgaaatacc gatgtctgcc tctaagaaat cttcacggat tttttggcgg acgaatgcag
     4021 tcatctcttc aggtgtttcc gaaccttgat aacctagcac atcgtgcatt tcttggcgga
     4081 ttctgtggcg atctttgtgg attgcaggta caacaatatg agaaggttta tcgcccacaa
     4141 tttgcaataa atattcgccc aaatcggttt caaccacttt aatgccttct gcttctaaaa
     4201 cgtggttcat cccaatctct tcggttacca tcgacttcga ttttacgatt ttcttcgcat
     4261 ttttagcttt cgccacgttt ttgatgtaat cagtggcttc ttcggcggtt tcggcaaaga
     4321 aaacgtgtcc gccgttttgg gtgacttttt cgcttaattg gtagagataa gcgtctaaat
     4381 tggctaaaac gtggttacga attgctttcg catcatcacg ccactcttcc cagttgccca
     4441 gctcatccac catacgctga cggtttgcac cgattgtttc ctgagcctta actaaggctt
     4501 tacgcacaat ctcgttatta acttgctgct caacacgttg tttaaacggc agggtgttag
     4561 tttgtaaata agacatctta ttttcctccc tgcattaaca cttcggcaat gtgcatcact
     4621 ttgattggct taccttcacg gcttaaacga ccgccaatgt tcatcaagca gcttacatct
     4681 gccccgatta aataatccgg ctcatcggta tctacatttg cgactttctc ttttaccatc
     4741 tcgccggaaa tttctgccat tttgaccgag aatgtgccac caaaaccaca gcaagtttct
     4801 tggtttttga ttggtaataa ctctaaccct ttaacgtgct gtaataaagc aataggctca
     4861 tctttaatcc ccagcttacg gaataagctg caagacgggt gataaactgc tttgcccggt
     4921 aaataagccc caacatcttt cacgcccagt ttgttcacaa taaaatcggt taaatcatag
     4981 aaacgatcag ccactttctt cgcacgttct gcccattctt tttcgccaaa gcgttcaaaa
     5041 tgatccggat aggttttgat cgcatataca caagaacctg ccggggcgac aatcgggtaa
     5101 tcattagctt caaaggtttc aaccaagttt ttcatacctg ctaaagcttg cttagtgtaa
     5161 ccgctgttga tcgcaggttg tccacagcaa ccttgttttt ctaagaaagt gattttacaa
     5221 cctaattttt ccaataaaag cacagtattt tttgcaaccc ctgctttcat tacatctgca
     5281 atgcaggtta cgtaaaaatt aacgttcata aagaactccc acagtaaata taaataagaa
     5341 ttaaactcaa aaaatgccgt tctaagacgg cgaaaggaag aggtggattt gcaaaaaatt
     5401 tgctaaatct caccgcttcc tttctaaatc aagtaagctg attattaact cattataggt
     5461 tatagaacat aggtactata aacaatgccg aaatcgcagc aataacgcca tacacgaaca
     5521 ttggcacggc tgttttctta ataatattgc cttcttgttt ctcaatgccg gtaacagaac
     5581 acacagccac gatgttgtta atacacacca tattccccat tgcaccacca actgattgca
     5641 atgcaagaat taaggtaaca gataacccga cttgctgtgc cgttgcatat tgtactgttc
     5701 cgaaggttaa gttagatacc gtatttgacc ctgagaagaa cgcaccaatt gcacctaaga
     5761 acgaggcaaa taatgtccaa gattgaccgg taaattctgc gaagttttta ccaatgatct
     5821 gaaccattga gtctacacca ccaattaaca ttaattttac catcacaatg gcacctaaaa
     5881 gtgctaagaa tggttttttc acttgtttaa atgaagtgcc aaaaatctct tgagtatttt
     5941 tacctgatac cgagaaaatc ggtaagcaaa ttaatacggt cacaacaaat ggaatcaacg
     6001 caggaacata cagtaattta tagctagttg ctacattcgt acctaaaata tttttcagtg
     6061 caaaaattaa gccttggcta atttcaaagt tagcgaaacc aagcgaactg acaatccaag
     6121 ctgttgcatc atttagcatt gcttttaatg gaagttgctg aatacgggtg attaccaaaa
     6181 tgaagatcag taaccctgtt ggtaataatg cttttgccac ttcaccaaag gtcgctttat
     6241 cgccgtcaag gtggttctcg cttttcgcta aaccaatacc catatttgct acccagactg
     6301 acaccaataa accaatcgcc ccggcaacta atgatgggaa ttcgtagtta aattgagcaa
     6361 taatgaagta aggaatgaca caggcaaaaa tgctgatata aataaacacg atattttgac
     6421 gaatttcttt ccagctcaca attaaacgca atgccataac tggaataata aacgcagcaa
     6481 tagagtggat cagtgcagta atagaaccaa tttcaagcac ttgtgcatcg gttagtaaac
     6541 catcttttaa taatggtccc ataccaaacc aagttggtgt gccaaccgca ccaaatgaca
     6601 ccggcacaga gttcattact aaggctaaca ttgccacttg taacggttta aagcctaaac
     6661 caaccagaat tggagctgca attgccgccg gcgtaccgaa acctgatgcg ccttcaatca
     6721 taaatgcaaa tgcccaaccg ataatcatca actgtgccac cggattcggg ttaattgtcc
     6781 ctaaccattt acgcatagtg ttggttgcac ctgaaacttc agagaaacgg ttaaataaaa
     6841 tcgccccgaa aataacggta attggggtta atacatcacc taaaccggca ataatattgg
     6901 caaagattaa cgtggtatca ttaccaaaat aggtaagctg aagcacaaaa attaataacg
     6961 ccgtaagtgg taaagccaca taagacggta acgcgttacg ttttaccatt aaataaatga
     7021 gcaaaacaat tggaaatacg ctcaggaata gagccatact gcacctcttt atatgattat
     7081 aaaatgacct aactggtcgg ataggtgcga attgtactct taaaaaaaac taatgtaact
     7141 aggttgttac aattttgtga tctagct
//
LOCUS       DANLXQ010000131         7163 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.131, whole genome shotgun sequence.
ACCESSION   DANLXQ010000131 DANLXQ010000000
VERSION     DANLXQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.131"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..227
                     /locus_tag="PXD94_002047"
     CDS             <1..227
                     /locus_tag="PXD94_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL6286876.1"
                     /translation="GYRVGSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSSDVE
                     LGAEYTRDNLKRSGTKLKGNAFTANATYRF"
     gene            complement(305..1534)
                     /gene="serA"
                     /locus_tag="PXD94_002048"
     CDS             complement(305..1534)
                     /gene="serA"
                     /locus_tag="PXD94_002048"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL6286877.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(1571..2230)
                     /gene="rpiA"
                     /locus_tag="PXD94_002049"
     CDS             complement(1571..2230)
                     /gene="rpiA"
                     /locus_tag="PXD94_002049"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL6286878.1"
                     /translation="MDQQEMKKIAAQAALKFVKPDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            complement(2375..3709)
                     /gene="glmM"
                     /locus_tag="PXD94_002050"
     CDS             complement(2375..3709)
                     /gene="glmM"
                     /locus_tag="PXD94_002050"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL6286879.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGPMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            complement(3735..5267)
                     /gene="ilvA"
                     /locus_tag="PXD94_002051"
     CDS             complement(3735..5267)
                     /gene="ilvA"
                     /locus_tag="PXD94_002051"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL6286880.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            complement(5528..6121)
                     /gene="slyD"
                     /locus_tag="PXD94_002052"
     CDS             complement(5528..6121)
                     /gene="slyD"
                     /locus_tag="PXD94_002052"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL6286881.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            complement(6224..6364)
                     /locus_tag="PXD94_002053"
     CDS             complement(6224..6364)
                     /locus_tag="PXD94_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286882.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            complement(6436..7122)
                     /gene="aqpZ"
                     /locus_tag="PXD94_002054"
     CDS             complement(6436..7122)
                     /gene="aqpZ"
                     /locus_tag="PXD94_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL6286883.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHISGGHFNPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
BASE COUNT         2026 a         1740 c         1346 g         2051 t
ORIGIN      
        1 aaggttatcg tgttggatcc gatttattac cttatgtaaa agtagatgcc agctcaagca
       61 aaatcggtaa tgaaacctat cgtggctttg gtttcggtgc tggtgcaaaa tatgcagttt
      121 caagcgatgt tgaattaggt gcagaataca ctcgcgataa tttaaaacgt agcggcacaa
      181 aattaaaagg taacgctttc accgcgaatg caacttatcg tttctaatct tagcggtaaa
      241 gttttctgaa aaaataaccg cttgtaacac aaaagagcct gaagtgattc aggctctttg
      301 ccttttaata taatactcgg gctcgaatcg tgccttcgat cgcttttaaa cgttgtaatg
      361 cttcatcaac attgtcgctc tctacatcaa ttacaacata accgatagct gcatcggttt
      421 gtaaatattg agcggcaata ttcacatttg attccacaaa aatctggtta atttgattta
      481 acatacctgg gcgattctcg tgaatatgta ataaacgttt ggtattagta tgaagcggta
      541 aggatacttc agggaaattc actgccgtca cggttgaacc gttatctgaa tatttcacaa
      601 atttattggc aacttcggtg ccgatatttg cctgagcttc agaggttgaa ccaccaatat
      661 gtggcgttaa aatcacgtta tcaaacttgc acaatggtga cacgaacggg tcattaattg
      721 atgccggttc ttccggaaat acgtcaatcg ccgcaccacg caatgagccg tcttcaatcc
      781 gagcggctaa tgcgtctaaa tcgaccactg taccacgagc ggcattaatt aacaccgagc
      841 cttcttttaa ttgagcaatg cgttctgcac tcattaggtt tttagtcgag tcgatttccg
      901 gaacgtgtag tgaaatcata tcgcaggttg aaagcaaatg ctctaatgtc ggcacttgct
      961 gtgcattacc gagtggtagt ttgttttcaa catcatagaa ataaacctgc ataccaatgg
     1021 cttcggcaag aatactaagc tgtgaaccga tatgaccata tcccacaata cctaatttct
     1081 tgccacgtac ttcgtttgca cctgctgccg atttattcca caccccacga tgcacttccg
     1141 catttgcagt cgggacttta cgcattaata aaatcatctc tgccagcact aattctgcca
     1201 ccgagcgggt atttgagaac ggtgcattga ataccggaat acctcgtctt ttcgccgcat
     1261 tcaaatcaac ttggtttgta ccgatacaga aacaaccaat cgcaattaat ttcggagctt
     1321 gagccagtac ttcttcggtt aaaaaagtac gcgagcgaat accaacaaag tgtgcatctt
     1381 taattgcatc aattaattct tgaccgtcta atgctttttt atgcacttca atattggtat
     1441 aaccagccgc taagagtaca tcaattgcgt tttgatgtac gccctcaagc agaaggaatt
     1501 tgatcttgga tttatccaaa gaaattttag ccattttttg ctcctaagta tttgtatttg
     1561 caataataaa ttattctaaa ataactgcac cttcaggtgt gccgacaatg gttacattgg
     1621 caaaacgttg tgcaaaaata ccgttagtca ccacgcctgc aatattatta atggtatgtt
     1681 ccattttcaa cggctccata attttaaagt tatgtacatc taaaatcaca ttgccgttat
     1741 cagtgaccac gccctcacgg tattccggcg aaccacctaa ggctacgagc tgacgagcca
     1801 cataagaacg agccattggg atcacctcca ccggcagagg gaaagttgaa cccagcacat
     1861 caacttgttt ggatttatcc acaatgcaga taaatttttt cgctaatgaa ctcacgattt
     1921 tctcacgggt aagtgctgcc ccgccacctt taatcatcgc cccttgtggc gtgatttcat
     1981 cagctccgtc aatatacaca tctaattccg tcacttcatt ggcactgaat acttcaatgc
     2041 cgatatcacg caaacgctgt tccgaggctt tagaggcagc aaccgctcct ttaatctcat
     2101 ctttcattga agcaagggca tcaataaaac agtttacggt tgaaccactt cccaccccaa
     2161 caatagtgtc cggttttaca aattttaatg cagcttgtgc cgcaattttt ttcatttctt
     2221 gttgatccat ttttacctcc acttgtaggg caaatcatca tttgcccact tcggcaaggc
     2281 gaaaaaaatt ttcgccctac tacatctatt ctacacatat atgtaacaag cggtcatatt
     2341 tggcaaattt tttaccaaaa acgaccgctt gtcattagtt acgttttact gcttcaacaa
     2401 tctcttccgc acagctttga gcaagtacag catcttcgca ctccaccatc actcgaatta
     2461 atggctcagt gcctgattta cgcaataaaa tacgcccttt gcctgccaag cgtttttcca
     2521 catctgccgc taccgctttc acttcgttac tttcaagcgg atttgccgcc cctttctcaa
     2581 agcgaacatt tagcagaact tgcgggaaca gaggcactgc ttttgccaaa tcattcaggc
     2641 ttaatttgtg gcttgccatt gctgctaata cttgcagtga ggcaataata ccatcaccgg
     2701 tggtgttttt atctaacacg ataatatgcc cggagttttc gccacccagt ttccagcctt
     2761 tttctttaag ctgctctagt acatagcggt cacccacgtt agcacgagag aatggaatgg
     2821 ctagctcttt taaagctaat tccaagctca tattactcat taatgtaccg actacaccgc
     2881 cttgtaattt gcctgaacgt agagcttctc gggctaaaat aaatagaatt tggtcgccat
     2941 caactttatt gcctaagtga tcgaccataa tcaaacggtc gccatcacca tcgtaagcta
     3001 gaccaacatc cgcacctgct tctaccacca ctttttgaag tgctttaata tccgttgcac
     3061 cacatttttc gttgatgttt aaaccgtccg gcttagtacc gatttcaagc acttctgccc
     3121 ctagctcacg cattacattc ggcgcgatat ggtaggttgc accgttggca caatccacca
     3181 caattttata gccttcaagg cttagttcag acgggaaagt gcctttacaa aactcgatat
     3241 aacgtcctgc ggcatcatta atacgactgg cacgacctaa atttgctgaa tctacgcaat
     3301 ccataggctt atcaagcatt gcttcaatcg cttcttcaat ttcatcaggt agtttttcac
     3361 caaccgacga gaagaattta atgccgttat cgtaataagg attgtgagag gcagaaatca
     3421 caatgccggc ttccgcacgg aaagtacggg ttaaataagc aattgccgga gtcggcatcg
     3481 gaccaacgaa tactgctgat aaaccggctg cggttaaacc ggcttccaga gccgattcca
     3541 acatataacc ggaaatacgg gtatctttac cgattaatac ttttttcgtg ccttgggttg
     3601 ccaacacttt acctgctgcc caaccgagtt ttaatgcaaa ttcaggtgta attggaaatt
     3661 tacctacttc accacgcaca ccgtctgtgc cgaaatattt acgagccatt ctaattccta
     3721 agattgataa aaatttactt taaaaaatat tgatatgccg gactttcgct cacattttgg
     3781 aagcggtaac ctagctgttc taaatgtcgg tttaattcta cttcatcgcc tctgtttaat
     3841 gcaaatgcgg caagaatatc gccataatcg gctccgtgag ctcggtaatg gaataacgag
     3901 atatcccagt tagtcagcat ttgtaagaat tttagcaatg cccctttctg ctctggaaat
     3961 tcaaagctat agagttgctc atattgaatg gagcttgctc taccgcctac catataacga
     4021 atatgggttt tggcaatatc atcgtccgat aaatctgcca catcatagcc gttttgttgc
     4081 agatctttaa taatctctac tttttctgcc gagcctgaaa ttcgcacccc cacaaaaata
     4141 caggcttgtt tatcatcgga atggcgatag ttaaattccg ttaccgcacg gttgcctaaa
     4201 atctcgcaga attttaagaa actacctttc tgttcagcaa tggtgactgc caataaagct
     4261 tcacgtttct ccccgatttc acaacgctct gacacaaaac gtaaggtgtg gaaattcaca
     4321 ttcgccccgg aaagaatgca agctaagttt ttatcttgta attgatgctc tttcacatat
     4381 tttttcaaac cggcaaggga agttgccccg gacggctcgg aaatcgcacg cacattttca
     4441 aaaatcaact tcattgaggc acagatttca tcattatcca ccaacaccac atcatcaaca
     4501 tattgctgac acaggcggaa agtttcatcg ccaatgcgtt ttaccgcaat accgtcagca
     4561 aaaagcccaa cccgttctaa atcggtcggt tgcccttgct ttaaggcgtg atataagcaa
     4621 gcagaatctt tagattcgac cccaatcact ttaatattcg gcatcacctg cttaattaaa
     4681 accgcaatgc cggcaattaa gccgccaccg cccactggaa taaatacata atctaaatcc
     4741 ggcttttgtt gcaccagttc taagccgatt gtgccttgcc ctgcaatcac tgccgggctg
     4801 tcaaacgggt gaatgaaggt catacccaat tcggtggaaa gctcaatcgc tttcgcctta
     4861 gcttcatcaa aattcgcccc ataaagcaac acttcaccac catagccacg taccgcatca
     4921 accttaattg ccggtgtgtt ttgcggcatt acaatcaagg ctcttaaacc taaatgtttg
     4981 gcagaaagtg ccaccccttg tgcgtggttg cctgctgaag ccgcaatgac tccagcctct
     5041 ttttgtgctt tggataaatt tgagatcatc gcaaaagcac ctcgcaattt aaagctgtga
     5101 accggctgtc ggtcttcacg tttgataaaa atagagttgc ctaaccgctc agacagtttt
     5161 tccattttct gtaaaggggt cacttgcgct aagtcataaa tatttgagct taaaatcgag
     5221 cgtaaatagt cgatagcgga ttgggaatgt tgtgtttcgt tcatcatttt gaaaggttat
     5281 ttattgttag attgagataa agacattggt aaatttccaa ggaaaattga ccgcttgttt
     5341 ggttatttag caatggcttt aacagggaat atttcctctc gcccgtttgc gggagagaga
     5401 cagcttaaaa tttcgttcag aaattttaag cagagagagg ggaaaccttg gatatccccc
     5461 tctccctccg caaatcttcg atttgctcca ccctctcccg caagcgggga gaggggaaat
     5521 cacaaagtta gtgatgacca ccgcaaccac agccaccgtg tccgtgatga tggtgatgat
     5581 cgtggtcgtg accgccgcag caaccatcgt gatcgtgatg atgatcgtgc ccaccacaac
     5641 cgcaaccgtg ttcgtgtccg ccgtgaacgt gtccgtgtgc gatttcttcc gctgtcgctt
     5701 cacgaatgcc aaccacttcc acagagaaaa gtaattcctg accggctaac atatggttac
     5761 cgtccaccac aacttcatcg ccatctactt cggtaattac caccggtagc ggacctaaat
     5821 ccgtgtccgc aataaaacgc atacccactt ctaattcatc gacgcccata aatacatctt
     5881 tcggtacacg ttgaaccata ttatcgttgt actcgccgta accttcttcc ggtttcacac
     5941 gcacttcaaa cacatcgcct acagctttgc cttcaagggc tttttctaag ccttctacca
     6001 agttgttgtg accgtgtaaa tattctaacg gttggtttac cggtgcttca tctactaata
     6061 cgccttcttg agtacgcact tgatatgcaa tgcttggcac taaatctttt gtaatgttca
     6121 tattgaatcc tttaattcat cggttttata aaatagcaag ccattgtaac gctttatttt
     6181 atatttgcaa tctatctgga aattttaacc gcttgtcagt aaattagtcg ataatatcag
     6241 gggttacggt gtctaccaca tcaactgcgg ttcccaccac tccaccaacc accgaagtta
     6301 ccaaacaacc acttaggaaa atagttgctg aaatcagccc acatactttg attaattttt
     6361 tcattttaaa atcctttcaa aaacaaaaaa cagccctaat caagactaga gctgcatcat
     6421 atttattcgc ttaaattatt tttcttcagc gataaagcga taaactaccg caccgataat
     6481 tgcaccgaca atcggagcaa cccagaacaa ccataattgc tcaatcgccc agctgccttg
     6541 gaataacgct acaccggttg aacgtgccgg gtttactgag gtattggtta ccggaatgct
     6601 gattaagtgg attaaggtta aagccaaacc aatagcaatc ggagcgaaac ctgctggagc
     6661 acgtttatcg gttgcaccca taatgatgat taagaagaat gccgttaata ccacttcaat
     6721 taataatgct gctgttaagc tatagccgtg tggcgaatgc tcaccataac cgttgccggc
     6781 aaagcctgct gtagcatcaa aagtcggaac acctgaagca atggtgtata acactgcacc
     6841 ggcagcaatc gcaccgatta cttgggcaat gatataaggc actaaatctt tagcgttaaa
     6901 acgaccgccg actaataaac cgattgaaac cgccgggttg aagtgaccac ctgaaatatg
     6961 accgactgca tacgccattg ttaatacggt taaaccgaag gctaacgaaa cgcctgcata
     7021 accgattcct aattcaggaa tgcctgctgc taaaactgca ctgccgcaac caccaaacac
     7081 taaccaaaat gtcccgaaaa actcagcaaa atattttttc attttttatc cttaccctaa
     7141 agggtgaatc acacataagt gta
//
LOCUS       DANLXQ010000132         7051 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.132, whole genome shotgun sequence.
ACCESSION   DANLXQ010000132 DANLXQ010000000
VERSION     DANLXQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.132"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            162..830
                     /locus_tag="PXD94_002055"
     CDS             162..830
                     /locus_tag="PXD94_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6286884.1"
                     /translation="MAHNGSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKH
                     IAYLPQRLPEAADFLVQELVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQP
                     VNSLSGGEKQRVWLAMCLAQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVI
                     IIIHDINLASQFCDEIIALKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHK
                     VAVL"
     gene            827..1708
                     /locus_tag="PXD94_002056"
     CDS             827..1708
                     /locus_tag="PXD94_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6286885.1"
                     /translation="MMFRQYKRFIFLFILQISTAFAQPNIFTPDWSIASALTEMGNPP
                     IAMGDKRIYPLWSHRPIQPNSVLDIGARYQPNRELLAQLPVELVLSNFFYAHLASVYP
                     KEIPQIDVLFDGGNTEPQQHWHTYQKATRMIGTEIKNPNAAEAYLTKTEQTLAQWGKE
                     ILNSNPDIDYYSVVQFGSPRELRIYASNSMFRVAFDLMGLKQADLGVGDRWGNKLIPV
                     SDLAKLPERTCLIVISPFSKMTQADLENSYIWQRLGFGKNRCMKVLPPVWLFGGADSI
                     SHFAEFLHNAMTQGEKE"
     gene            1708..2523
                     /locus_tag="PXD94_002057"
     CDS             1708..2523
                     /locus_tag="PXD94_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL6286886.1"
                     /translation="MKSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDLLPINQ
                     AAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALMIAVIF
                     FPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIASLLTL
                     FYFDFLFGVMVWGSGSLLQDGWATSITLAITVVVAFFIFVLLSRPLTILSLDDEQARR
                     LGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAVQVWLILPKYDIYYFAS"
     gene            2520..3656
                     /locus_tag="PXD94_002058"
     CDS             2520..3656
                     /locus_tag="PXD94_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL6286887.1"
                     /translation="MIAFLAGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLI
                     FLVLRQRKAQFEASNTAYFPVKRLNSWKLPILLLCAGVILFCLLQIIAPTMNGWAISI
                     DENLMSSYRLPRSLSAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGATIAAVLSF
                     LLFPTLSSISFLLFGVLGSLTVLALIMWLSRKLQPSALMITGVAIGALVSGLMAIIQL
                     SGSPRLTAVISWLSGSTYYANPNTVWILLSVAILLISATLLFIKPLRLISLGETVSYH
                     LGLEVKKVQMIVLLLIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPEKQIPISAL
                     LGAMLMLIADWLGRYLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            3684..4082
                     /locus_tag="PXD94_002059"
     CDS             3684..4082
                     /locus_tag="PXD94_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL6286888.1"
                     /translation="MNTLSKAIKLALYSSGIGFISTAYAETVTHLDTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LLDRGRYSFSARGFGIDKVKVDGMDLKVSK"
     gene            4117..5820
                     /locus_tag="PXD94_002060"
     CDS             4117..5820
                     /locus_tag="PXD94_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL6286889.1"
                     /translation="MLYDRVEVVRGATGLTTGAGDPSASVNLVRKRANSISRYTILEA
                     GIGRYADFNTMIDHSQALNNSGSVRGRFIAEHKNGNTFIKNEKERYTTLYGNIEADLT
                     DTTKLGLGVSYQKEKRDAALWGGLPAFYSDGGETNWSRSKNASPEWAYWDNESVNYFA
                     DITQKLGEKWELALKGNYRVGKHNSELFYFSGSSLNRQDGLGWSPWPGKFKNDNTQSN
                     VQLDLTGSFSAWGLEHDAAIGIQYNRAHRNSYAADNSTFDGAFDFNNWNGIYARPNWG
                     SMSEKYYQNLKEFGVYVSTRLHLTDRLSMLLGSRISSYRTEGTFYGSTQNFHANDVWT
                     PYAGLTFDINPQNTVYVSYTDIFKPQSQVDINNKLLSPVTGETYEAGWKGSYFNDNLQ
                     TQISVFETRQDNLAQSAGINPNTLLAYYTASKGAKVRGFEVEASGNVTEKLKLSAGYT
                     QWKGDDASGNPINTTQPRKQFKLFATYDMNRLVQGLTVGAGVNWQSRIYTKHATYGEY
                     GQKAYTLVNLMARYQFNDNFSAQLNIDNLFDKKYRNALSFSQYSYGEPFYTRLNLRYE
                     F"
     gene            5932..6576
                     /gene="adk"
                     /locus_tag="PXD94_002061"
     CDS             5932..6576
                     /gene="adk"
                     /locus_tag="PXD94_002061"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL6286890.1"
                     /translation="MKVILLGAPGAGKGTQAQFIMNKFGIPQISTGDMFRAAIKESTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKESGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNPPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLIAYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
     gene            complement(6638..>7051)
                     /locus_tag="PXD94_002062"
     CDS             complement(6638..>7051)
                     /locus_tag="PXD94_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL6286891.1"
                     /translation="KAFNDAIVAIWLDPKMDISHQLLDPSYKPRAEAESIKPQVEERV
                     AENLQEPIVNTEVSKELKADSQAVEQAPNKIDIPVADKTQASVVNEATQPVENKDKKE
                     PEKLEEKPKEMKIENPEVFISPIYDDILMLTKYCV"
BASE COUNT         2044 a         1392 c         1421 g         2194 t
ORIGIN      
        1 ctactcattt tcattttgat tgatttggtg gggtaatccc acctttttta tgctcttaaa
       61 acacaacact atgattcaaa taaattccat tagctactct gttgaacaaa accgtaaact
      121 gctaagcaat atttccctca atttaagcaa ggggcgttta tatggcgcat aacggctcgg
      181 gtaaatcaac gttaatgcgt ttactaagcg gagatataac gcctaccagc ggttcaattt
      241 tagtcgatgg attaccactt catcagcttt ctaccaaaga agcggcaaaa catattgcct
      301 atttaccaca acgtttacct gaagcggcgg atttcttagt gcaggaactg gttgctttag
      361 ggcgatttcc atatcaaaaa tggctacaaa aaccaacaga atttgatgtt caaattgttg
      421 agcaagcaat ggaaagcacc tccgttactc agtttgctaa acagcctgta aatagtctat
      481 caggcggaga aaaacaacga gtttggcttg ctatgtgctt agcccaacaa acaccctatt
      541 tactgctaga tgagccattg gctgcccttg atattgttta tcaagttgaa gtaatgcagc
      601 ttattcgcaa gctagtgaat gaacaaggtc taactgtcat tatcattatt cacgatatta
      661 atcttgcctc ccaattttgt gatgaaatta ttgcgttaaa gcaaggcgaa ctctgtattc
      721 aaggttctgt tgatgaaatt atgcaagaac agaaattact tgagatcttc gggctaaaac
      781 tgcacttgtt taaccaccct gcaggcttac ataaagtggc ggtattatga tgtttaggca
      841 atacaagcgg ttcatttttc tctttatttt gcaaatttct accgcatttg ctcaacctaa
      901 tatttttaca ccagattggt ctattgcctc tgcccttact gaaatgggaa atccgcccat
      961 tgcgatgggg gataagcgaa tttatccgct atggtcgcac cgccctattc agcctaatag
     1021 cgtgttagat attggagcgc gttatcagcc taatcgcgaa ttattagcac agcttcctgt
     1081 tgaattggtg ctatcaaact ttttctacgc tcacttggct tctgtttacc cgaaagaaat
     1141 tccacaaata gacgtgctat ttgatggtgg caatactgaa cctcagcaac attggcatac
     1201 ttaccaaaaa gccactcgta tgattggaac agagattaaa aaccctaatg cggcagaagc
     1261 atatttaacg aaaactgaac aaacgttggc tcaatggggg aaagaaatcc ttaacagtaa
     1321 tccagatatt gattactact ctgttgttca atttggctct ccgagagagt tacgcatata
     1381 tgcgtctaat tcaatgttcc gtgtagcctt tgatttgatg ggcttaaaac aagctgattt
     1441 aggtgtggga gatcgctggg gaaataaact tattcctgtg agtgatttag ctaaattacc
     1501 cgaacgcacc tgtttaattg tcatctcacc tttttctaaa atgacgcaag cagatttaga
     1561 aaatagctac atttggcaac gtttaggatt tggtaaaaac cgttgtatga aagtattacc
     1621 gcctgtttgg ctctttggtg gggctgattc catctcgcac tttgctgagt ttttacacaa
     1681 tgcaatgaca caaggagaaa aagagtaatg aaatcatcat tcttattgat tggctgtatt
     1741 cttttatcct tagtaacagc tgttatccgt ttaaatatgg tctgggagtg ggaatggcag
     1801 ttactactca taaatgttga tttattgcca attaatcaag ctgcggtaaa agcagctctt
     1861 ttacctgaaa tgacaacgtc atttttagca ggcggtttat tggctatagc cagcagtggc
     1921 ttacaacata ttgtcagaaa taatttagcg tcagatagca cacttgccgt cagtagcggg
     1981 gctcaacttg cattgatgat tgcggttatc tttttcccag ccgccgagct tttttctagt
     2041 ttctggattg cgttacttgg ctctttagct gctatcgtac tggttttatt tattgctaaa
     2101 gcctctaagc tcaatcccct tacccttatt ttagggggat tgattgttaa tattttgttt
     2161 ggagctattg cctctttact gacccttttc tactttgatt ttttatttgg cgtgatggtt
     2221 tggggaagtg gctcactttt acaagatggt tgggcaactt ctattacctt agcgataacg
     2281 gtagtagttg cattttttat ctttgtctta ctctctcgtc cactcacaat tttaagttta
     2341 gatgatgaac aagctcgccg cttaggcgcg cctgttaatt tactacgcta cttagttatt
     2401 tttgtttgtg ccgccattac agcattggtt gtaagcaaaa taggtgtgat tggctttatt
     2461 ggatttgcag tgcaagtttg gctcattttg ccaaaatacg acatttatta cttcgcctct
     2521 tgattgcttt tctagcgggc ggatttttac tcttttttac caataatctc atcggcattg
     2581 ttagcagaat ttacgacact ctactacctg ttggtgcatt taccgcaaca ctaggcgcac
     2641 cattattaat tttcttggta ttacgccaac gtaaagcaca atttgaagca agcaatacag
     2701 catacttccc cgttaaacgc ctcaattcgt ggaaactgcc aatattgttg ttatgcgctg
     2761 gagttatcct attttgtcta ttacaaatca ttgctccaac aatgaatggc tgggctatta
     2821 gcattgatga aaacctgatg agttcctacc gcttgcctcg tagtctaagt gcgatagcaa
     2881 caggggcaat gcttgcggta agtggtagcc tattacaaat cttaacgaga aacccaatgg
     2941 caagccctga agtattaggt gtgagttcag gggcaactat tgctgcagta ctgtcattct
     3001 tacttttccc aacattatct agcattagct tcttgttatt tggtgtatta gggagtttga
     3061 ctgttctcgc tcttattatg tggctctctc gtaaattaca accatcagcg ttaatgatta
     3121 caggtgttgc tattggagct ttagtcagtg gcttaatggc tattattcaa ctaagcggca
     3181 gccctcgttt aacggcagtt attagttggc tgtctggctc aacctattac gctaatccga
     3241 atacagtttg gattttatta tctgttgcaa tcctacttat cagtgccact ttactgttta
     3301 tcaaaccttt acgtttgatc tcacttggcg agaccgtttc ttaccattta gggttagagg
     3361 ttaaaaaagt acaaatgatt gtattacttt taattgccct aatgagtgcc gcctctacac
     3421 ttgcggtagg gccaatgagc ttcattgggc taatgacccc tcatttagct aaaaatttag
     3481 gtgcaattac accggagaaa cagatcccta tatcggcatt attaggggca atgctaatgc
     3541 tcattgccga ttggctcggg cgttatctaa ttttccctta tgagattggg gcaggcatta
     3601 ttgcctctat tctcgggggt atctatttct tactgttact caaacaacat aaataatttt
     3661 ttattcatta cttaggagaa attatgaaca cgctatctaa agcgataaaa cttgccttat
     3721 acagttctgg tatagggttt atctcaacag cttacgctga aacagttact catcttgata
     3781 ccgtagaagt aagtgccgaa acgccaaatg cctcatcgga agaaactgga cgttatgcac
     3841 agaaaaaagc caaaggctct aatggaatgg cattgagtgc gaaagacacg ccacaaagcg
     3901 taacggctgt aacgcatcaa caaatcagag atcaaaatct taatacgatt gccaaagcac
     3961 ttgaagcaac tcacggtgtt tctgtttctt tacttgacag aggacgctat tctttcagtg
     4021 ctcgtggttt tgggattgat aaagtcaagg tagatggtat ggatctcaaa gtaagtaaat
     4081 aaccaatgga caacgggcga aaatacaggc aacagcgtgt tatacgaccg agtagaagtt
     4141 gtgcgtggtg ctacaggctt aactactggg gcaggcgatc cttctgcaag tgttaatctt
     4201 gttcgtaaac gagccaatag catttcccgt tatactattt tagaagctgg aatcgggcgt
     4261 tatgctgatt tcaatacaat gattgaccat agccaagcat tgaataattc aggatcagta
     4321 agaggccgct ttattgcaga gcataaaaac ggtaatacgt tcattaaaaa tgaaaaagaa
     4381 cgttacacca cgctttatgg caatattgaa gcagacttaa ctgacactac caaacttggg
     4441 ttaggtgtaa gctatcaaaa agaaaaacgt gatgcagcac tttggggtgg tttacccgca
     4501 ttctatagcg acggtggtga aactaactgg agcagaagca aaaatgccag tccggaatgg
     4561 gcttactggg ataatgaatc cgtaaactat tttgccgata ttacacaaaa actaggcgaa
     4621 aaatgggaac ttgcattaaa aggcaattac cgtgtaggca aacataattc agaacttttc
     4681 tatttcagcg gctcatcact taatcgccaa gatggattag gttggtcgcc ttggccgggt
     4741 aaatttaaaa atgataatac acaatctaat gtgcagcttg atcttaccgg ttcattctct
     4801 gcttggggat tagaacacga tgcggcaatc ggtattcaat acaatcgagc acatcgtaat
     4861 tcctacgctg ccgataatag cacctttgac ggagctttcg actttaataa ttggaatggc
     4921 atttatgcta gaccaaactg gggaagtatg tctgaaaaat actaccaaaa cttaaaagag
     4981 ttcggggtgt atgtttctac ccgtttacat cttacagata gactttctat gcttctaggt
     5041 tctcgtattt catcgtaccg cacagaaggc actttctatg gttcaacaca aaacttccac
     5101 gctaacgatg tttggacacc ttatgcagga ttaacctttg atattaatcc acaaaatacc
     5161 gtttatgtaa gttataccga tattttcaaa ccacaaagtc aagtggacat caataacaaa
     5221 ctactctctc ctgttacggg cgaaacctac gaagcaggtt ggaaagggag ttatttcaat
     5281 gacaacttgc aaactcaaat tagcgtattt gaaacaagac aagataatct agctcaatct
     5341 gccggtatta accctaatac attattagcc tactacacag cctctaaagg tgcgaaagtt
     5401 cgaggctttg aagtagaagc atctggaaat gtaactgaaa aattaaaact cagtgcagga
     5461 tatactcagt ggaaaggcga tgatgcttca ggtaacccaa ttaacacaac tcaaccacgc
     5521 aaacaattta aactctttgc aacttacgat atgaaccgtt tagtgcaagg tttaacggta
     5581 ggagcaggtg taaactggca aagcagaatt tataccaaac acgctactta tggcgaatat
     5641 ggtcaaaaag cttacacttt agtgaatttg atggctcgtt atcaatttaa tgacaacttc
     5701 tcagcacagc ttaacattga taacttattc gataaaaaat atcgtaacgc cctcagcttt
     5761 agccaatata gctacggtga gccattctat acacgcttaa atttacgcta tgaattctaa
     5821 ttccgtataa caagcggtca aaaaattaaa aaaatttgca attttaggca gtaatcggta
     5881 aggttactgc ctaagtgatt ttattatttt tagtttaatt gaggttttca aatgaaagtg
     5941 attttattag gtgcgccggg tgcgggcaaa ggtacgcaag ctcagtttat tatgaacaaa
     6001 ttcggtattc cgcaaatttc aaccggcgat atgttccgtg cagcgattaa agaaagcact
     6061 gagcttggta aacaagcaaa agcattgatg gacgaaggca agttagtgcc ggatgaatta
     6121 acggtcgcct tagtaaaaga tcgtattgct caaccggatt gtgctaatgg cttcttatta
     6181 gacggtttcc cacgcaccat tccgcaagct gatgcgttaa aagagtcagg tgtaaaaatt
     6241 gatttagtgt tagagtttga tgtagctgat gaagtcattg ttgagcgtat gagtggtcgt
     6301 cgtgttcacc aaccttcagg gcgtacttac cacgtggtgt acaatccacc gaaagtggaa
     6361 ggcaaagatg atgtgactgg tgaggattta attatccgcc aagatgacaa acctgaaacg
     6421 gtattagagc gtttagcgat ttaccacaaa caaaccaaac cgttaatcgc ctactacacc
     6481 gcagaagcag aagccggcaa cactcgttat gagcgtttag atggaacaaa accagtggaa
     6541 gaagtaagtg ctgagttagc gaaaatttta tcttaaatgg attattaaaa tgaacaagcg
     6601 gtaatatttt tcgagaatat taccgcttgt tctttgctta aacacaatat ttggtgagca
     6661 ttaagatatc atcataaatc ggtgaaataa acacttccgg attttcaatc ttcatctcct
     6721 ttggtttttc ttctagcttt tctggctctt tcttatcttt attctctacc ggttgagtcg
     6781 cttcatttac cacactagct tgagttttat ctgctacagg tatatctatt ttgtttggtg
     6841 cttgctctac tgcttgagaa tccgctttca gctctttaga aacttcagta tttacgatag
     6901 gttcttgtaa gttttccgct actctttcct ctacttgtgg cttaatactt tctgcctctg
     6961 ctctcggttt ataagatgga tcaagtaatt gatggctaat atccattttc ggatcaagcc
     7021 aaattgctac aatagcatca ttgaaagctt t
//
LOCUS       DANLXQ010000133         6981 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.133, whole genome shotgun sequence.
ACCESSION   DANLXQ010000133 DANLXQ010000000
VERSION     DANLXQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.133"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            170..742
                     /locus_tag="PXD94_002063"
     CDS             170..742
                     /locus_tag="PXD94_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6286892.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGTTKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(809..1267)
                     /locus_tag="PXD94_002064"
     CDS             complement(809..1267)
                     /locus_tag="PXD94_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6286893.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(1441..1824)
                     /gene="grcA"
                     /locus_tag="PXD94_002065"
     CDS             complement(1441..1824)
                     /gene="grcA"
                     /locus_tag="PXD94_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL6286894.1"
                     /translation="MIKGVQITESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVV
                     AISELGDIAYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVITRTFTESL"
     gene            1983..2672
                     /gene="ung"
                     /locus_tag="PXD94_002066"
     CDS             1983..2672
                     /gene="ung"
                     /locus_tag="PXD94_002066"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL6286895.1"
                     /translation="MKTWTEALGEEKQKAYFQQILQYVHNERLAGKVIYPPQNEVFSA
                     FALTEFSDVKVVILGQDPYHGPNQAHGLAFSVKPPVAPPPSLVNMYKELSEDIEGFQI
                     PPHGYLIDWAKQGVFMLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLL
                     WGSHAQRKGQFIDRNRHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQ
                     LSVDVSYSSTH"
     gene            2688..3608
                     /locus_tag="PXD94_002067"
     CDS             2688..3608
                     /locus_tag="PXD94_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL6286896.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGYHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTSS"
     gene            3644..4081
                     /locus_tag="PXD94_002068"
     CDS             3644..4081
                     /locus_tag="PXD94_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL6286897.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQIALFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIARGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(4222..6858)
                     /locus_tag="PXD94_002069"
     CDS             complement(4222..6858)
                     /locus_tag="PXD94_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL6286898.1"
                     /translation="MDEGFMVKHPRHSSHLHPLIHQLTTPEGEVRIYDASYPQFEVNP
                     VEKEDGIDLIPSLETHGAGVAGIIAAQADKTLGDGYSGGIAKGAELYVATKSYKRTLE
                     KVIQDAKKELENAKDEEDEKTPSLDQMAKNDLLASKEKEMAIERAEWASGLNKLLDNN
                     VFAINNSWNPFSISDDINVVDKFYQSIKQNKHNPLLQAIMRAKNSNTLLVFAAGNESK
                     KQPGVMALLPRYFPELEKNLISAVAVDKEQKIASYSNHCGASKNWCVAAPGDLHVLIG
                     VADEHKKPQYGLTKEQGTSFSAPAITASLAVLKERFDYLTATQIRDTLLTTATDLGEK
                     GVDNVYGWGLINLKKAVNGPTQFLNDETITVTRDDHWSNPLASQFKITKKGDKSLHLD
                     GENHLDTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLEVLGKSG
                     LIANAQANIHLAGSLKIDDKLTEKTEAGDVSATVVQLKDKATYQGGFTQLVENENLAK
                     RGLIQDLYFKESEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRSWYNGWLQ
                     SALEQRKLDNLHYAVSNNIYADSLELLRSQNAKGLTQAQQHLFTAYHTPLQTTVWAEH
                     LNQKQSASSKHTDVKHHQSQLGVNHKLADKTVLSATLSQQKNRLEKPFAQATLKQTAL
                     NIGLRYHLDNAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRIGYQFMP
                     NQWIIEPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQLEQGSI
                     SPYIGLNYLHRLNGKITKITSNIAGKTLHSEATTKRNRQLNGEVGVKLHYKNWFTAMN
                     LDYSRVKSANLFGWKVNVGFSF"
BASE COUNT         2001 a         1284 c         1370 g         2326 t
ORIGIN      
        1 gcagatgaaa taaaatttct acgcacattg gaactaaaat aggttatact ggtctgagta
       61 gtgggtctag cagagtctag accttatttt gttatctttt ttaatctttt agaagattta
      121 gttaaggaat ttaccgctgt aattgttctg gcgagacgag acataaatta tgaaaaaaaa
      181 tctttttgca acaaccttca agcccgtatt tattgcttct attttgtctg tttttggagg
      241 gtattcttcc attgctgtag caagtttaag ttctaaaccg gccacaccaa atcaaatcat
      301 tgcaagtaaa gcagaaagat tagctaagtc ttcttcagta tttaccaatc gagcaaatct
      361 tccaacttct tccgctcata cagcgaatta tagtggtcgt attaatagtg tttatcataa
      421 ttgggtaggg acacgctatc gtttaggcgg cacaacaaaa gcaggaattg attgttccgc
      481 ctttgtgcgt gaagtaatgg gcaaagcatt tagtaaagat atgcctcgtt caactgcaga
      541 acaacgccat gtcgggcatt ccatttcaaa atctgattta cgcccaggtg atttagtctt
      601 tttccgtaag aataaccatg taggggttta tattggtaat ggtaaatttg tacacgctag
      661 ttcaagtaga ggggtaacca ccagctcatt gtcagaaagc tactggtcaa gaaactacac
      721 acaatctcgc cgtgtaattt aataaattac aattccaaga tgaaaagaac actcactttt
      781 gcgagtgttc ttttttaatt aaaaaagttt atgctacgcg ttgcttagct tgtgtaaatg
      841 ctccacgagt acgcatttga tgaaccacat tagtaattac acctgctatt tttacttcag
      901 cccaatcagt aattctaaga tttgattcgc tcacatctaa tgaaaataaa attttttcag
      961 caataccttc cccattctga atttcattaa agaattgata gaatttatat tccccatctt
     1021 tttcaagcac caatagatca ttaactaaat attggttggt ctgttctaca atcaataaat
     1081 cgtctagttc aattccccac gcgattaaat ttgggttttt cacgtggata aaaaaagtgt
     1141 ctcttgggcg tttgatacaa agtgagttaa gatcgagctt tacagagcgg ctgctattga
     1201 aatcccgata aagcggaatc ggctgataag ctgccgtttg atctgaaatt tgtctaattg
     1261 aagccatagt aaaatcctct atctgttttt ttatacaggg tattttactg gctaaaatac
     1321 cagtgtcaac cattattttg gaattttttg taaatttaac cagtgttttg accgcttgtt
     1381 atcctttaaa aagcaataaa aaagcaccca tagggtgctt cttatgctta aaagctgtta
     1441 ttataggctt tcagtgaatg tacgagtaat aacatcgcgt tgttgctcag gggttaatga
     1501 gttgaaacga actgcataac ctgaaacacg gatcgttaat tgaggatatt tctcagggtt
     1561 ttgaatcgca tcttctaatg tttcacggcg taaaacgttt acgtttaagt gctgaccacc
     1621 ttcaacttta atcgttggtg caacattaac gggtacttca cggtatgcga tatcacctaa
     1681 ttcgctgata gcaacaactt gatcttcttc aaatcctgct tttgcagcta aacaacgagc
     1741 ttcattttta gcttcgtcta ataaccagaa agagtttaat aagttgctgt ttgatgattc
     1801 tgtgatttgt acacctttaa tcattttgat atctcctcta agattgaaat tataaaaaaa
     1861 ttttaaaaat tcaaatttat ttgaagttat tgtacccctt tttagataaa aagcgataaa
     1921 aatttaacca tttttagaca tgattgagtt ggttgggtaa aaaatataca cttggtggtt
     1981 ttatgaaaac ttggacagaa gctttaggcg aagaaaaaca aaaagcgtat tttcaacaaa
     2041 ttttacaata tgtacataac gaacgattag ccggcaaagt catctatcct ccgcaaaacg
     2101 aggtgtttag tgcttttgcc ttaaccgaat ttagtgacgt gaaagtagta attttagggc
     2161 aagatcctta tcacggccca aatcaagccc acggattagc cttttcagtt aaaccgccgg
     2221 ttgccccgcc accttctctg gtcaatatgt ataaagaatt atctgaagat attgaaggat
     2281 ttcaaattcc accgcatggc tatttaattg actgggcaaa acaaggggta tttatgttaa
     2341 acaccagctt aacggttgag caaggtagag ccggttcgca ttcacaaatt ggttgggaaa
     2401 tttttaccga taaagtgatc gcacaattaa atcaacaccg tgataattta gtctttctac
     2461 tttggggaag ccacgctcag agaaaggggc aatttatcga tcgtaatcgc cattgtgtgt
     2521 tagcttctcc gcacccatca ccactttccg catatcgtgg attttttggt tgtaaacatt
     2581 tttctaaagc caataactac ttgatttctc aaggaatatc cccaattaat tggcaattat
     2641 cagtagacgt tagttattct tcaacacatt agtttaagga gcaaacgatg aattttgatt
     2701 taccgattgt tagtattgca tttgttgtgc ttgttttagt tgcattaagt tcgactatta
     2761 aaattgtgcc acagggctat cattggacag tagaacgttt tggtcgttat actaaaacgc
     2821 taagcccggg gttgaacatt gtcgtacctt ttattgatcg tattgggcga aaaatgaata
     2881 tgatggagca agttttagat attccatcgc aagaggttat ttcacgtgat aatgcaagtg
     2941 ttgcgattga tgctgtatgt tttgttcaaa cagttgatgc tcgccgagct gcctatgaag
     3001 tgaatcattt agagcaagca attgttaatt taaccatgac aaatatgcgt accgtgctcg
     3061 gctctatgga tctagatgat atgctctctc agcgtgattt aattaacggt agattacttt
     3121 ccattgtcga tgaggcgact aatatttggg gagtaaaagt cacccgtatt gagattcggg
     3181 atgttcgtcc accgaaagag ctggttgcgg caatgaacgc tcaaatgaaa gctgagcgta
     3241 ataaacgtgc tgatattctt gaagcagaag ggattcgaca agctgaaatt ctgcgggctg
     3301 aaggggaaaa acaatctcgt attttaaaag cagaaggtga aagacaagaa gcattcttac
     3361 aggcagaagc tcgtgaacga gccgcagaag cggaagctaa agccactcaa atggtttctg
     3421 aagcaattgc taaaggcgat actacagcga ttaactattt tattgcacaa aaatacaccg
     3481 aagctttgaa agaaataggt tcttcagata acagtaaagt tgtacttatg ccgttagaag
     3541 ctggaaattt gattggttct gttgctggta ttgctgaaat tttaaaaagc aataaaacaa
     3601 gctcatagcc taaaaccgga ttaattgagt atatggaggg agtatggact ggttttttaa
     3661 ttggattggc tggagcatat taggctttgt actacttgca ttagaattag tattaccggg
     3721 tgtctttatt atgtggtggg gatttgcctc gcttattctt gctttcatcg ttgcactttt
     3781 tacattaggg caagcgttac aaattgcgtt atttgcgata attgcgacta tttttagtct
     3841 tatttggtgg aaatatcagc atggaaaaga caagaaagag gatcagagca gttcccttaa
     3901 ctctcgagag catgctatgc taggagctca aggctttgtg gttgaaatct tacctaatgg
     3961 catcgctcga ggaaaatttg gtgatacgac ttggcgtgtt ataggtaata atttacaagt
     4021 agcagataag attgaagtcc agaaagtgga gggaattacc ttatttgtcc gtaagcaata
     4081 agttttttct tatttagtca tagaacacta aacaaaaacc ttgaacagtt gttcaaggtt
     4141 ttttctctca agcggttgaa aatagagtga aatttgcaaa ttttcaccct aaattaaccg
     4201 cttgtgtccc accataataa gttagaaact aaagccaaca tttactttcc aaccaaatag
     4261 gtttgcagat ttcactcgac tgtaatctaa attcatcgca gtaaaccaat ttttataatg
     4321 cagtttcacc ccgacttcgc cgtttaattg gcgattacgt ttcgtcgttg cttcactatg
     4381 cagtgttttt cctgcaatgt tactggtaat tttggttatt tttccattta atctatgcaa
     4441 gtaatttagc ccgatataag gggaaataga gccttgctca agttggaaag ttcgctgtaa
     4501 tttcacgctt ggaacaatat tcacatcacg ataacgcatt gcagccgttt gagtcgcaag
     4561 ttcgccactt tcatttaaac cattcatttt ggtttgaatc cattgtacgc caaggcttgg
     4621 ttcaattatc cattggtttg gcataaattg ataaccgatc cgcatttcac caccgagcgt
     4681 tgaacctctg gtttctgcag tacctagttg atggctagca aatcgacggc tttgttgata
     4741 tttctggcga gcaaattgca atgtagcttc actaaaccaa gcgttatcta aatgataacg
     4801 taagccaata tttaatgctg tttgttttaa ggtagcttgt gcaaatggtt tttctaagcg
     4861 gtttttttgc tgtgaaagtg ttgcactaag taccgtttta tctgccagtt tgtggtttac
     4921 tcccaattgt gattggtggt gtttaacatc ggtatgcttc gatgaggctg attgtttttg
     4981 attcaaatgt tcagcccaaa cagtcgtttg cagtggtgta tggtatgccg taaacaaatg
     5041 ttgctgtgct tgggttaagc ctttggcatt ttggctacgt agcaattcaa ggctatctgc
     5101 ataaatattg ttagatacag catagtgcag attatctaat ttacgttgtt cgagtgcaga
     5161 ttgcagccaa ccgttatacc aactacggcg ataggcaata ggtgtagttc ttaacgcatt
     5221 taataatgct aaaccggctt gcccgtttgt atcggctttc tcatcagtta gtggcttatt
     5281 cacttttgcg ataatttcgc tctctttgaa atagagatct tggattaacc ctcgtttggc
     5341 taaattctcg ttttcgacta attgtgtaaa tccgccttga taagttgctt tatctttcaa
     5401 ctgtactacg gtcgctgaaa catcacctgc ttcggttttt tctgttaatt tatcatcaat
     5461 ttttaacgag ccggctaaat gtatattggc ttgagcattg gcaattagcc cagatttgcc
     5521 caacacttct aattggcttt gtccagacga agaataattt tgctcaactt cggttccatt
     5581 gacagctaaa tttgcatggt tactgattgt tttcacctta gttttaccgt ttaaggccaa
     5641 tctaccttct tcaactgcta ccgtatctaa atgattttcg ccgtctaaat gcagtgattt
     5701 gtcccctttt tttgtaattt taaattggct ggcgagtgga ttcgaccaat ggtcatcacg
     5761 agttacggta atcgtttcat catttaggaa ttgagttggg ccattaacag cttttttcag
     5821 gttaatgaga ccccagccat aaacattatc gacccctttt tcacctaaat cagtggcggt
     5881 agtcaataaa gtatcgcgaa tttgagtcgc agtcagataa tcaaagcgtt cttttaatac
     5941 tgctaatgaa gcagttatag caggtgctga aaatgacgta ccttgctctt ttgttaagcc
     6001 atattgtggt tttttatgtt catcagcaac gccaattaaa acatgtaaat cgcccggtgc
     6061 tgctacacac cagtttttac ttgctccgca gtggttagag tatgaagcaa ttttttgttc
     6121 tttatctacc gcaacggcag aaattaaatt tttctcaagc tcgggaaagt aacgtggtag
     6181 aagagccatt acgcctggct gtttcttgct ctcattacca gcagcaaaga ctaataaggt
     6241 gttgctattt ttggctctca taatagcttg aagtagcgga ttgtgcttat tttgcttgat
     6301 agattgataa aatttatcta caacattgat gtcatcgcta atagaaaaag gattccaact
     6361 attattgatt gcaaaaacat tattatctag taatttattc agcccggatg cccattcagc
     6421 acgttcaatt gccatttctt tttctttgct tgctaataag tcattctttg ccatttgatc
     6481 cagacttggc gttttttcat cctcttcgtc ttttgcattt tctaattctt tcttagcatc
     6541 ttgaataact ttttctaaag ttcgtttata tgacttcgtt gcaacataaa gctcagctcc
     6601 ctttgcaata ccaccactat agccatcacc aagagtttta tctgcttgag cggcaataat
     6661 accagctaca cctgcaccgt gtgtttcaag agaaggaata agatcaattc catcttcttt
     6721 ttctacgggg tttacctcga attgagggta acttgcatcg tagattctta cttcgccttc
     6781 gggtgttgtt aattgatgaa taagaggatg aagatgtgaa ctatggcgag ggtgtttaac
     6841 catgaagccc tcatccatta cgcctagttt aacacccttg ccactatatt ttgagctgag
     6901 gctctcagat aattgaatga taggttgtgg attctctatt gattctgtag cgtatgcaat
     6961 agataaaaaa ctagaaatgc t
//
LOCUS       DANLXQ010000134         6974 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.134, whole genome shotgun sequence.
ACCESSION   DANLXQ010000134 DANLXQ010000000
VERSION     DANLXQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.134"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            141..1259
                     /gene="malK"
                     /locus_tag="PXD94_002070"
     CDS             141..1259
                     /gene="malK"
                     /locus_tag="PXD94_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL6286899.1"
                     /translation="MANVSLRNVGKSYGDVHISKDINLEINEGEFVVFVGPSGCGKST
                     LLRMIAGLEDITTGELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKL
                     AGVSKAERDQRVNQVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEP
                     LSNLDAALRVQMRVEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKP
                     LELYHYPANRFVAGFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVN
                     VGDNLSLGIRPEHLVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQND
                     VVLVNEGDDLNIGILPERCHLFKEDGTACKRLFVEKGV"
     gene            1357..2646
                     /locus_tag="PXD94_002071"
     CDS             1357..2646
                     /locus_tag="PXD94_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HDL6286900.1"
                     /translation="MKKTVLAVAISGAMFAASASAVDFHGYARSGIGWTSGGGEQSAF
                     TVNGGGSKYRLGNESDTYAELKLGQELFKSGEKSIYFDTNLAYGGTLHNNDWTPTSPA
                     LRELNVQFKNFADSLPGATLWAGKRFYQRHDVHMNDFYYWDISGPGAGVENIDLGFGK
                     LSLAVTRDTERGGANTFGYTTVYKLDPSTGKIKLDKQRNKADVYNDIFDVRLAGIELW
                     KDGSLELGFDYGNAHAKKGAALEKGATKNGYMVTAEYTQGNFFGGFNKFTAQYATDSM
                     TSWNNGHAQGSKADNKGNMLRLINQGVVQASDKVEVMYALIYEKTKLDNKQGKTWYSA
                     GIRPMYKWNDTMSTLLEVGYDRIKDQATGKKNDLVKYTVAQQWQAGNSIWARPAIRVF
                     GTYARWNDKFNTKARTDAGYKAKDGEFITGVQFEAWW"
     gene            2727..3623
                     /gene="malM"
                     /locus_tag="PXD94_002072"
     CDS             2727..3623
                     /gene="malM"
                     /locus_tag="PXD94_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL6286901.1"
                     /translation="MKKTLLSTAIFFAHLLVVSPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(3783..5651)
                     /locus_tag="PXD94_002073"
     CDS             complement(3783..5651)
                     /locus_tag="PXD94_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL6286902.1"
                     /translation="MSGLVSPELIKTVALLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
     gene            complement(5682..>6974)
                     /locus_tag="PXD94_002074"
     CDS             complement(5682..>6974)
                     /locus_tag="PXD94_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL6286903.1"
                     /translation="KFHINPSIMTNDTDILMRKLYSVFEALSGKISLINHKISSNLAE
                     KAITFIEAKVGSALQPGWYLVNQAPKSRYDSENRYVHYNKSPIKLIAWGYFNGIINAA
                     TNLHTVSPNLDIEKLRQFITDLRLSFPIQAPEVTNDDMHHPNEIRNLVIALNLVQDPT
                     EHIDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQHFDGEDAILKALKLV
                     SNRIYQGNSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQTGCLLQENEFSTLKVA
                     GKTWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEKKKEFPQQISEFASEG
                     FLQFFFEDNVDNSFNVYVVDERNNVESYYNCFGNKEEKIRKINKLQNEKQLKTETPAS
                     FGSFNHPQFYQLISPNGELCIVPFQSRQHLDYLAQKHH"
BASE COUNT         2217 a         1332 c         1390 g         2035 t
ORIGIN      
        1 taggatgatt tcttctttag ataaaaaggc gactattcag cctaaaaaaa agtattttag
       61 gatgatttct tctttagata aaaaggcgac tattcagcct aaataaaacc aacaagcaaa
      121 agcagaaaag aggtttagtt atggcgaatg tatcattacg taacgtggga aaatcgtatg
      181 gtgatgttca tatctctaag gatattaatt tagaaattaa cgaaggagaa ttcgttgttt
      241 tcgttggacc gtcaggttgt ggcaaatcta cgctattacg aatgatagca ggtttggaag
      301 atattacgac aggagaactc tatatcggtg gaaaactgat gaatgaagta gagccttcaa
      361 aacgtggtat cggtatggtg ttccaatcct atgctctcta tccacattta gacgttgccg
      421 aaaatatgtc ttttggctta aagctggcag gtgtaagtaa agcagagcgg gatcaacgag
      481 ttaaccaagt tgccgaaatc ttacagcttg cccatttatt agatcgaaaa cctaaagcac
      541 tttccggcgg acaacgccaa cgggtggcta ttggtagaac actggtttcc caaccggaag
      601 tatttttact cgatgaacca ctttccaacc ttgatgccgc attgcgtgta caaatgcggg
      661 tagaaatttc caaattacat aaaaaactaa accgcacaat gatttatgtt acgcacgatc
      721 aagttgaagc aatgacatta gcggacaaaa tcgtggtatt aaatgccggc ggtattgctc
      781 aagtaggtaa accgttagaa ctttatcatt acccggcaaa tcgctttgta gcagggttta
      841 tcggctcgcc taaaatgaac tttttaccgg taaaagtgat tgcggtagag ccggaacggg
      901 taaaaattga gttacctgat gcgaatcatc ataatttttg gattccggtt tccggcgaag
      961 gggtgaatgt gggagataac ttatctttag gtatccgccc ggaacattta gttccttcgg
     1021 atcaaaccca agtgacctta cacggtattg tgcaagttgt ggaattgctc ggaaatgaaa
     1081 cacaaatcca ccttgaaatc cctgaaatca aacaaccaac cttaatttat cgtcaaaacg
     1141 atgtggtatt agttaatgaa ggcgacgatc tgaatatcgg tatattgccg gaacgctgcc
     1201 atttatttaa ggaagacggt acagcttgca aacgtttgtt tgttgaaaaa ggggtataac
     1261 aatttcagtt tattactgat tttagagggt attttcaatc ttctaatcaa ttaataatta
     1321 acctaagtaa aatcaaatct taagagaggt tctattatga aaaaaacagt cctagcagtt
     1381 gcaatcagtg gtgcaatgtt cgcagccagt gcatcagcgg ttgatttcca cggatatgca
     1441 cgttcaggta tcggttggac atcggggggg ggggagcaat ccgcattcac ggtaaatggt
     1501 ggtggttcaa aataccgttt aggtaacgaa tctgatacct atgcagaatt gaaattaggt
     1561 caggaattat tcaaaagtgg tgagaaatct atctactttg atacaaacct tgcttacggc
     1621 ggtacgcttc acaataatga ttggacacca acaagtccag cactacgtga attaaatgta
     1681 cagttcaaaa actttgcaga tagtttacca ggtgccacct tatgggctgg taaacgtttt
     1741 taccaacgcc atgatgtaca tatgaatgac ttctattact gggatatttc aggtccgggt
     1801 gcaggggttg aaaatattga tttaggcttc ggaaaactct ctttagcggt tactcgtgat
     1861 acagagcgtg gaggggcgaa tacttttgga tatactacag tttataaatt agatcctagc
     1921 acaggtaaaa ttaaattaga taagcagcgt aataaagccg atgtttacaa cgatattttt
     1981 gatgttcggt tagccggtat tgagctttgg aaagatggtt cgttagagtt aggctttgat
     2041 tacggtaatg cacatgctaa gaaaggtgca gcccttgaaa aaggagcaac caaaaacggt
     2101 tatatggtaa cagcggaata cactcaaggt aacttctttg gtggtttcaa taaatttact
     2161 gcacaatatg caaccgactc tatgacttct tggaataatg ggcacgcaca gggttcgaaa
     2221 gcagataata aaggtaatat gcttcgttta atcaaccaag gggtagtgca agcgagtgat
     2281 aaagttgaag taatgtatgc cttaatctac gaaaaaacaa aactagataa taaacaaggt
     2341 aaaacttggt actcagcagg tattcgccca atgtacaaat ggaatgatac aatgagtaca
     2401 ttattagaag ttggttatga tcgcattaaa gatcaagcaa caggcaagaa aaatgacttg
     2461 gtaaaataca ccgttgcaca acaatggcag gctggtaata gcatttgggc tcgccctgca
     2521 atccgtgtgt ttggtactta tgcacgttgg aatgataaat tcaacacgaa agctcgtacc
     2581 gatgccggct ataaagccaa agacggcgag tttatcaccg gtgttcaatt tgaagcttgg
     2641 tggtaatcat caaacctttg tgtgggtagg attacctacc cactatttca gcactttatc
     2701 ctatttcacg atttttaggg gttaatatga aaaaaacgct tctttcgaca gcaatattct
     2761 tcgcccatct tttggtggtg tctccgagta tagcaaatgg ctcggcaggt aagtctattc
     2821 atattaactc ggctgaatta gctcaaattc aatggcaaga tgtgccattt tcacaaaccg
     2881 taaaaacaga tctaagcgag caacacaaac aagcatttac cacagcgttt gccggtattg
     2941 ccagcccggt tgcagcttat cgtattccgg caaatcaagg cacattggaa attgaaatcg
     3001 aaagcccggt gatggacaaa aatgtttttg tacccacggc agtcgtgttg gatagtcaat
     3061 ttaatgtggc atcaacttac ccctcttctg agtttaaatt attggaagag cgaggactca
     3121 aaggtagccg tttaggggca gaacttaatt taacgccggc aatgaaccaa gattatattt
     3181 acttgctcat ttataccacg cagcaagatt tagccaaaac cacgatgatg ccgcatccgg
     3241 caaaaaccta tgcaaaagca accggtaaac aaccaccagc aattaacgat attgaagtag
     3301 cacacagttt aaaagggcaa attcacatca acgttaccgg ctcgaacggc actaaattta
     3361 ttggattacc gactgaaatt ttcagctcaa acaaagcagc aacgccggca gctaaaacgg
     3421 ctataccgtt gccgataaaa gaaaatccag ttgcagtaaa aacagcagta gataaagata
     3481 cggaagccta ttttaatcag gcggtaatga aagcgattaa agcaaaagat attaataaag
     3541 cgatgaattt ggttaacgag gcagaaaaat tagggctgac ttctccacgc caaattttct
     3601 taaaaaatgt aagttcaaat tgatttattg tttttttcat agatattctc cttgtcatcg
     3661 tgcgatggtt tattaccatt aacaccctat cagattatgc cgatagggtg cttttttacg
     3721 agtgattatg cgatatgtat aggaaggctt atacttgaat aaggattcaa agtataaatt
     3781 ctttaatttt tatcagtgat atccactttt ataacttcta ctgtctcacc aagctcttct
     3841 aaaatatcag ccgtttcttc attaagtgca ctaccttcgt gttgtggatt tacccaaggt
     3901 gttggtaccc aatatttgcg gataacttct gcggagaaac cgtgtaataa ttcctcatta
     3961 aagcgttctg catctaatcg gcgaacatag ctgataattt gttttgcctc ttcctcgcta
     4021 gaacctaagc gttcaatggc ggcttggctt aatgcgaggg ctgattcaaa ggtttctcga
     4081 atgtgataat caacgtggtg ctttttgact aaatcaaccg cactttgtct atcataggtt
     4141 ctcgcaaata caggtactaa ggggtattca gatttaatca gctctacgat tttctccgtt
     4201 ctttccggat tgcctacgcc aacaattacg cagtttgcat tggcgatgcc agatgcccta
     4261 agtacatcta gtctcgctcc atcaccatag taaagtttaa aaccaaaacg agcagcagca
     4321 cgaatatgat cagtatttga atcaataacc gatacactta gtcctttggc taataaggct
     4381 tggcagacta tttggttaaa gcgtccaaaa ccgatgacta atacattggc ttctaaatct
     4441 tccggtaatt caacccctgt catattagtg gattcatttt tcgctttttt ctttctcact
     4501 ccttgcttga ttaaaatgag aagtggggaa agcaacattg agacaattac cgcagcggtt
     4561 aaggtagcgt gagtatctgg ggttaaaatc ccagcggttg cggcggctga gaatagcaca
     4621 aaggcaaatt caccaccgtg agccataatg gtagttcgca caatagattc attcagtgtt
     4681 aatcgggtga tagaggcagc aatgaagatt ccaaggcctt taccgataac ataaactgct
     4741 acaatcacca ataaccatag ccaattattg aggacgaggc tgaggtctaa tgacattcct
     4801 acgcccataa agaataagcc taaaagtaaa ccacggaagg gttcaatatc agcttctagt
     4861 tgatggcgga aagaagactc cgaaagcatt actccggcaa ggaatgcccc cattgccatt
     4921 gaaagcccac tgatttccat taaaagagcg gagcctaata ccacaagtaa ggcagcggca
     4981 gtcatcattt ctcgtacttt ggctttagaa ataacacgga aaatagggtt catcagccat
     5041 tttccactcg caaccaaaat aagaacggca actaggctaa gaccaatagc agaccaatta
     5101 gtactggatt cagcatctac ttggttagga gcaagaaagg caaccgaggc taataatggc
     5161 acaatcgcta aatcttcaaa tagtaaagtt gcaacaatac gttgcccttt tggcgtggaa
     5221 cttatccctt tatcttccag cacttgcatt acaatggcag tagaagatag cgtaaaaccc
     5281 ataccggcaa taaatgccac ttctttggaa aaacctaata agtaaatgcc ggctaaggtt
     5341 aataaagtac cgcaaatcgc aacttgtaag aaacctcgcc cgaagatggc tttacgcatc
     5401 gcccaaagca gttccgggtg catttccaaa ccaataataa acaaaaacat aaccacgcca
     5461 agttctgcca tatgcacaac agcggcaggg tcttggaaca atccaatgcc cgatggcccg
     5521 attaaacatc ctgcgactaa ataacccaat acgcttccta aaccaaggcg tttaaatagc
     5581 ggtacaatag tgacgctggt tgcaagtaat gcgactgttt taattaattc cggactaact
     5641 aatcctgaca tagaattccc acctgtataa tataaataag attaatgatg tttttgtgct
     5701 aaataatcta aatgctggcg gctttgaaac ggcacaatac ataactcacc gtttggtgaa
     5761 ataagttgat aaaattgtgg atgattaaag cttccaaatg atgctggtgt ttctgttttt
     5821 aattgttttt cattttgtaa tttattgatt ttcctgattt tctcttcttt attgccgaaa
     5881 cagttatagt aactttcgac attattacgt tcatctacaa catatacatt aaaactatta
     5941 tcgacattgt cttcaaagaa aaactgtaaa aagccttcac tggcaaattc actaatttgc
     6001 tgcggaaatt cttttttctt ttcattttcg cttttagcat tttgcttggt ttttgcaaaa
     6061 gaatttaaat tttcaaccgc ttgttgttca tcaatttgtt taatgctaaa tgttttttta
     6121 tgaaaaagaa actgccaggt ttttcctgct acttttaaag tgctaaattc attctcttgc
     6181 agtaaacaac cagtctgaat tgtaatacat ttatgaacta aatctgccac aaaatcacgt
     6241 aactcactac gtagatattt actatagcaa aaaacattaa ctgattgagg tgaagaattt
     6301 ccttgataaa ttcgattgga taccaatttt agtgctttta aaatagcatc ttcaccgtca
     6361 aaatgttgag ttcgaatttc attccataca tttcgataaa taatgctcac actaccaact
     6421 aacgtttttt gtgccttacc aaaattaaac agatcgcttg gataaacttt ttttggtagt
     6481 tcatcgatat gttcggttgg atcttgtact aaattaagcg ctataaccaa attgcgaatt
     6541 tcattagggt gatgcatgtc atcattggtc acttccggcg cttgaattgg gaaagataag
     6601 cgcaagtcgg tgataaattg tcgcaatttc tcgatatcta aattagggct aacagtatgt
     6661 aaattagttg ctgcgttaat aatgccgtta aaataccccc aagcaataag cttaatcggg
     6721 cttttattat aatggacata acgattttca gagtcataac gacttttagg tgcctgatta
     6781 actaaatacc aaccgggttg taacgcagat ccaactttag cttcaataaa tgtaattgct
     6841 ttttctgcta aattggacga tattttatga ttaattaaag atatttttcc tgaaagtgct
     6901 tcaaatacag aataaagttt tcgcattaaa atatcagtat catttgtcat aatgctcgga
     6961 ttaatatgaa attt
//
LOCUS       DANLXQ010000135         6911 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.135, whole genome shotgun sequence.
ACCESSION   DANLXQ010000135 DANLXQ010000000
VERSION     DANLXQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.135"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(145..492)
                     /gene="frdD"
                     /locus_tag="PXD94_002075"
     CDS             complement(145..492)
                     /gene="frdD"
                     /locus_tag="PXD94_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL6286904.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
     gene            complement(506..898)
                     /gene="frdC"
                     /locus_tag="PXD94_002076"
     CDS             complement(506..898)
                     /gene="frdC"
                     /locus_tag="PXD94_002076"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL6286905.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFTTDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            complement(908..1639)
                     /locus_tag="PXD94_002077"
     CDS             complement(908..1639)
                     /locus_tag="PXD94_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL6286906.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            complement(1655..3457)
                     /gene="frdA"
                     /locus_tag="PXD94_002078"
     CDS             complement(1655..3457)
                     /gene="frdA"
                     /locus_tag="PXD94_002078"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL6286907.1"
                     /translation="MQSVNFDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
                     HTVAAEGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGC
                     PWSRREDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLD
                     ILTDNGEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMAY
                     RHGVALRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIG
                     KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFIC
                     ELAKAYEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANR
                     LGSNSLAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESW
                     SDIRNQMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKI
                     ELGFILDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYS
                     DVKITKSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            3790..4773
                     /gene="epmA"
                     /locus_tag="PXD94_002079"
     CDS             3790..4773
                     /gene="epmA"
                     /locus_tag="PXD94_002079"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL6286908.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
     gene            4855..5745
                     /gene="rarD"
                     /locus_tag="PXD94_002080"
     CDS             4855..5745
                     /gene="rarD"
                     /locus_tag="PXD94_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL6286909.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWSALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(5756..6367)
                     /locus_tag="PXD94_002081"
     CDS             complement(5756..6367)
                     /locus_tag="PXD94_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6286910.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGAVALGAFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLPHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVPGRVGLISGTMFGLMFG
                     IGGLSAAVLGFFADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(6395..6911)
                     /locus_tag="PXD94_002082"
                     /pseudo
     CDS             complement(6395..6911)
                     /locus_tag="PXD94_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         2018 a         1566 c         1336 g         1991 t
ORIGIN      
        1 atcttttttg cagatattta atacaaaacg atcgttttat cggcaaaatt tctaatattt
       61 gcaaattttc ttataaattc gaccgcttgt tagcatatta aaaatgaaaa gaccatctgc
      121 tttatacaag atggtctttc tcatttaaat ctgcacgact gcgatgatta ctagaattgt
      181 gtataagata gacaatccgt agaaaatcac attacctgct ggcgtgtgaa ctttcaggtc
      241 gtgtaatcca tgatggatac ggtgcattcc tgcccacata gggaaaatcg ctaataccat
      301 aatagcgagc ttgccgatcc aagtatgggc aaaggaaatg atattatctg gtgatactaa
      361 cccgaatggc agtaataaac cgataataaa gattaccaca gggaaaaata atgcacttac
      421 cattccccca gcactaaaaa gcaaccatac cggcggttca tttgaacgtt ttgggttttg
      481 atcaagactc attttcttcc ccttcttaaa tataaaccaa gattaatgca attacgctaa
      541 ttacgcctgt tgctgcccaa agaatatttc ttccaaccga gactggtaag tgtttacctt
      601 taaccatcac ggacatgacg gcaggtgtca tcacaaaaag tgttgccgca tgatataaca
      661 ttgctgccaa tgaaattatg tttaggataa caactaacgg attttgtaag aaatccacaa
      721 aatcggtagt aaatgttcct ttacttaacg caactgctcc ataaaataat acaatgcaga
      781 accaaacagt tggaagacag gtagcttcac gcaccatata cattttatag aagtcgagtt
      841 ttttccacca gctggctttc attcccctta cataaggtct acgttttgtt gctgacattt
      901 cttcctccta tctcggtttg atcattgaga taacataatc tttcgcactt tcaagtttac
      961 cttggtttac agccgaagca gggccgacgt gtttcggaca gacttccgag cagtaaccca
     1021 cgaaagtaca gctccaaaca ccgtttttgc tgctgagtag cttcatacgc tcttcacgac
     1081 cattatcacg gttatccaag ttatagcggt gagccaatgt aatcgcagca ggtccgataa
     1141 attcagggtt caaaccaaat tgtgggcaag ccgcataaca taaaccgcag ttgatacaca
     1201 ttgagaattg acgatatttc tctaattgtg ccggcgtttg tttagtacgc tgtccttccg
     1261 gtgctttatt atcaataata taaggtttaa ttgcctcgat gctatcaata aagtggctta
     1321 aatccactac taagtcacgc tcaatcggga agttcgccag cggctcaatt cgcataaaac
     1381 cgctgtaatc acgcaggaat gttttacaag ctaacttcgg cttaccgttt accatcattc
     1441 cgcaagaacc acaaatcgcc atacggcaag accagcgata agaaagttcc ggttctaatt
     1501 cgtctttaat gaagcccaat gcatctaaaa gtgaagtttg gctgtcatac ggcacttcat
     1561 atctgtctaa atgtggctcg ctatctgatt cagggttgta gcgtagcact tcgatggtca
     1621 ttttatctaa atttgccatt ttactactcc ttctctattg ttgtgcttga gcttctgctg
     1681 cttttttgac tttttcttgg gcttcagcct ctgcaccata cacacgttta gccggttgtg
     1741 atttagtgat tttcacatcg ctgtatttaa tcgtcggtgt accctctgca ttgtagtacg
     1801 cttgtgtgtg ttttaagtag ttcacatcat cacgctcaac atagtctaaa cgttggtgag
     1861 ccccacgaga ttctttacgc tctaccgcag agcaagcaat ggattgagcc acatctaaaa
     1921 tgaagcctaa ttcaattttg tagagtaaat cagtgttaaa cacacttgat ttatctttta
     1981 cgctgatgtt tttataacgc tcttttagct cttggatttt gtttacagtc gcttccatac
     2041 tttcttgggt acggtaaata ccacaacctt cttccatcgc atcacccatt tggttacgga
     2101 tatccgacca agattcatta ccctcttggt tcgctaatgc gtgcaagcga gccaccacat
     2161 cttttgcttg tgcatcaatt tgtggctggt tgcgagcagt cacttctaac gcacggcgag
     2221 ccgcattttc accggcaact ttgccgaaca ccacaagctc tgctaatgag ttagaaccca
     2281 aacggttcgc accgtgcaag cctgatgaag cacattcacc tactgcgaat aagcctttaa
     2341 ttgaggtttc cgcattcata tccacttcaa taccacccat cgtgtagtga accaccgggc
     2401 gaacaggaat cggcgcttta gccggatcga ccccttcata ggctttcgct aattcacaaa
     2461 taaacggtaa acgctcaagt aaatattttt cacctaaatg gcgtaaatct aagtgaacta
     2521 cgtccacgcc ttttgcggtt tttaaggtgt tccctttacg ccattcttgc cagaatgctt
     2581 gtgaaacctt atcacgaggg cctaattcca tatatttgtt ttccggtttg ccgataggtg
     2641 tttccggtcc taaaccataa tcttgcaggt aacggtagcc atctttatta actaaaatac
     2701 caccttcacc acgacaacct tctgtcatca aaataccggt atttggcaaa cctgttgggt
     2761 gatattgtac gaactccata tcacgcaaag ccacaccgtg acgatacgcc attgataaac
     2821 cgtcgccggt tacaataccg ccgttagtgt taaagcgata agtacggcag ccgccaccgg
     2881 ttgcaatcac aactgcattt gcattgattt gaacaagtgt gccttccatc atattcattg
     2941 ccacacaacc acgagcttcg ccgttgtcgg tgagaatatc cagcacaaaa tgctcatcaa
     3001 aacgcacaat attcggatat ttaatcgaag tttggaacaa agtatgcaaa atgtggaaac
     3061 cggttttatc cgccgcaaac caagtacgct caattttcat tcctccaaaa cggcgaacat
     3121 tcacttcacc gtcttcacga cgactccaag ggcaacccca gcgttctaat tgtgtcattt
     3181 caatcggcga atgctctaca aaatactcca caatatcttg ctcacataac cagtcaccac
     3241 caccgacggt atcattaaag tgattgtcga aagagtcggt atctttaatt accgccgctg
     3301 aaccaccttc tgctgcaacc gtatggctac gcattgggta aacttttgaa attaaagcaa
     3361 tttttagatt tggatttgct tcagccgccg caatagcagc acgcaagcca ccaccaccgg
     3421 cacctataat cgcgacatca aaattaacac tttgcataat gcactccgaa agttaaataa
     3481 cttaattcat taaaaagtaa tcatattgtg ccactatttg agcaatgaga taacctattt
     3541 ttacaaattt gtaacataaa aaatgtaatt ttgtgatcta cctcaaaaaa ggtaataaag
     3601 cttcataaag gtattgagaa tggctcttaa ttggacagtg atagaaagaa aatttctaat
     3661 gtttaaaatg agaaaattta atttgttgca aaaaagttaa aaccctctct ttatttttct
     3721 tgatcggtaa ctagggcaac tcactttttt aggctaaaat agcctcacat tttcaagcgg
     3781 tcatttttta tgaacaattt acaactcgat aatattgatt ggcaaccaac agcctctatt
     3841 cctcacctga tccaacgttc aaaaatcatt acggaaatcc gcaagttctt taccgatcga
     3901 gggcttttag aggtagaaac cccgatttta agcgaatttt cggtgacaga cgttcatctt
     3961 tcgactttca atacgcagtt tcaatcgcct ttttcccctg aggcgaaaac actccatctg
     4021 atgaccagcc cggaatatca tatgaagcga ctacttgctg ctgggagtgg accgattttc
     4081 caactgtgta aagtatttcg caacgaggaa gccggtaagc gacacaaccc tgaatttacg
     4141 atgctggagt ggtatcgccc gcattttgat atgtatcgct taatcaatga agtggatgat
     4201 ttactgcaac aaattttgga ctgcgaaccg gcagaatcat tcagctatca atttgtgttc
     4261 caaacctatg tcggattaga tccgctttct gccaccaaag cacaacttgt ggaaaaagcc
     4321 cgaaaacacg gcttgcaatg tgatgatgat gaacagcgtg acaccttgtt acaattttta
     4381 ttcagcgagg ttgtcgaagc caatatcggg caagaaagac caactgccgt ttatcatttc
     4441 ccttccaccc aagcggcatt agctcaaatt agctcggaag atcaccgtgt tgccgaacgt
     4501 tttgagtttt actacaaagg cttggaactc tgtaacggct tccacgaatt agacgatgct
     4561 gatgagcaaa ttcatcgctt cgagcaagat aatctgcaac gtgaaaaaat ggggctggag
     4621 ccacagcaac tagatgtgcg ttttcttgcc gcactcaaag cgggttttcc aaactgctca
     4681 ggtgtcgccc ttggggttga taggctgtta atgctggcaa tggatgcgga aaaaattgaa
     4741 gaggttattt ctttcggcat tgaaaaagcc taatttttta tgccgttttg tgatttgcaa
     4801 tttgcaaaaa aatcgacaaa tatgaccgct tgtacactcg tttaggaaag caaaatgaat
     4861 atttcaggca aattacaggg ttggcactat gccctactgt gctacttaac ttggggaacc
     4921 tttccgattt attggtatcc gatcaaccaa tcgggaatgc ctgccgagca aattttggct
     4981 caacgggtgc tttggtctgc cctatttgct gtgtttctgc tgctgatttt ccaacaaggg
     5041 cgagcggtta ttaatgcctt taaacagcca aaggtactcg ggctgttcgt gctttcttct
     5101 tttatgattg gcgtgaactg gctggtttac ttatgggcaa tcgtcaatga gcatatttta
     5161 gatgccagtc taggttattt tatcaacccc attttcaacg tgtttgtcgg taggcttatt
     5221 ctaaaagagc agctgaataa agcccaatta ttggctctgc tgtttgcaat tagcggcatt
     5281 ttatggcttg ctattcctgc cggacaaatt ccgtgggtcg cattgattct agccgccagt
     5341 ttctgtattt acgggctgat tcgtaaactt gccccaatgg aacctttgtc tggtttggcg
     5401 ttagaaactt tgctgatgtc gccttttgcc atcacctatc tctgcttttg ttaccaccaa
     5461 aacattttgg tattcagtga actcaatcct ttacaaatgg cagtgctact ctgctccggc
     5521 attgccacta ccttaccatt gctatggttt gcaatgggtg caagacgcat ttcaatgtcg
     5581 ctactcggta tgctgcaata tatttcacct accctgcaat ttttatgcgg tgtgctgatt
     5641 tttaatgaga cactttccgc cgaacgtgct gtaggctaca ttctggtttg gattggggtg
     5701 ggggttttct tattgggtat gaaaaaagct ggtgtcaaac gctaaaaata aaacgttaaa
     5761 tatttcgttt tggtagccct aatgcagcaa aaccaagtag tggcaaatag gcacaggctt
     5821 taaaaatact ctccaagcca tattcatccg caaaaaaacc caatacggcg gcacttaagc
     5881 cgccaatacc aaacattaaa ccgaacattg tgccggaaat cagccctact ctgcctggta
     5941 cggcttcttg agcataaacc accatagcgg agaacgctga tgacataata aaaccggcaa
     6001 aaatcgcaca cacaatcgtc caaaagagat ctgcgtgagg caacaacaaa gcaaaaggtg
     6061 ccatacctaa gaaagagaac caaatcaccg catttcgtcc aatcttatcg ccaatcggcc
     6121 cgccgacaaa agcgcctaaa gcaactgcac ctaaaaaggt aaatagagag agctgtgcca
     6181 ttgctatatt gagttggaat ttttccatta aataaaaggt gaaatagtta ctgaggctcg
     6241 caatataaat aaatttcgct aacatcaata aagcgataat taataatgct ctggctaata
     6301 atttaccgtg taagcgttgt tgaatatggt ttggtgaaat aatgagcggt ttggcttcat
     6361 atttcaccca acggcttact ttatataaaa cccatcataa tgccaataat acaaatgcaa
     6421 caacccaaat caccgcactt tgccctaacg gcacaataaa taatgcaact aagagcggac
     6481 ctaacgccga tccactgttt ccacctactt ggaaaagcga ctgagctgta ccaaatttac
     6541 cgcccgatgc cattcgagcc acccgagagg cttcagggtg aaaaatggca gagcctatcc
     6601 ccattaacgc agcggctatc agtaacacgc caaaattcgg cgaaaaggca agtaaaatca
     6661 cggaaagaag cgtaatcagc atactgacgg gcaataaatt cggttttggg tatcggtata
     6721 aagcccaata cccggctgaa taatagatgc ggtaatttga taaatgagtg aaatcatacc
     6781 aatttgagca aaactcagtt ggtaattttc ttttagcatt ggatagatcg aaaccattac
     6841 tgcttgaact aaatcattca acaaatgaga aagggaaatc agcagtaaaa tgggcaaaac
     6901 cattttttga g
//
LOCUS       DANLXQ010000136         6789 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.136, whole genome shotgun sequence.
ACCESSION   DANLXQ010000136 DANLXQ010000000
VERSION     DANLXQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.136"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            119..1513
                     /gene="fumC"
                     /locus_tag="PXD94_002083"
     CDS             119..1513
                     /gene="fumC"
                     /locus_tag="PXD94_002083"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL6286911.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            1640..1750
                     /locus_tag="PXD94_002084"
     CDS             1640..1750
                     /locus_tag="PXD94_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL6286912.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            1855..3105
                     /gene="lysA"
                     /locus_tag="PXD94_002085"
     CDS             1855..3105
                     /gene="lysA"
                     /locus_tag="PXD94_002085"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL6286913.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHWHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            3119..3748
                     /locus_tag="PXD94_002086"
     CDS             3119..3748
                     /locus_tag="PXD94_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL6286914.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTARDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            3819..5264
                     /gene="tldD"
                     /locus_tag="PXD94_002087"
     CDS             3819..5264
                     /gene="tldD"
                     /locus_tag="PXD94_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL6286915.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNVLIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            5328..6209
                     /gene="prmA"
                     /locus_tag="PXD94_002088"
     CDS             5328..6209
                     /gene="prmA"
                     /locus_tag="PXD94_002088"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL6286916.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCRITGRKI"
     gene            complement(6384..6459)
                     /locus_tag="PXD94_002089"
     tRNA            complement(6384..6459)
                     /locus_tag="PXD94_002089"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6424..6426),aa:Trp,seq:cca)
     gene            complement(6525..6601)
                     /locus_tag="PXD94_002090"
     tRNA            complement(6525..6601)
                     /locus_tag="PXD94_002090"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6565..6567),aa:Asp,seq:gtc)
     gene            complement(6612..6727)
                     /gene="rrf"
                     /locus_tag="PXD94_002091"
     rRNA            complement(6612..6727)
                     /gene="rrf"
                     /locus_tag="PXD94_002091"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1954 a         1547 c         1546 g         1742 t
ORIGIN      
        1 aaggaattgc aattcgcagt tcctttcttt ttcttttata tgctagtatc accttatacc
       61 tctaaattat tagtgcctac tgattttcaa ttaatccaac aatttaggag agcgcatcat
      121 ggaattccgt atcgaaaaag acacaatggg cgaagtgcaa gttcctgccg ataaatactg
      181 ggcggcgcaa accgagcgtt cacgcaataa cttcaaaatc gggccggagg catcaatgcc
      241 ggcggaaatt atcgaggcgt ttggctatct gaaaaaagcg gctgcctatg ctaatcacga
      301 tttaggcgta ctagcggtcg aaaaacggga tttaattgca aaagcctgtg atgaaatttt
      361 ggcgaacaaa ctcgatgatc aattcccgct ggtgatttgg caaaccggct cgggtacgca
      421 gtcaaatatg aacgtgaatg aagtcgtggc aaaccgtgca cacgtgttaa acggcggcaa
      481 gctgggtgaa aaatcgatta ttcacccgaa tgatgatgtg aataaatccc aatcttctaa
      541 cgacacctac ccaactgcaa tgcacatcgc tgcttacaaa aaagtggtgg aacacaccct
      601 accttgtgtg aaacgtctgc aaaaaacctt agcggaaaaa gccgaagcct ttaaagatgt
      661 ggtcaaaata gggcgtactc acttaatgga tgctaccccg ctcacgctcg gtcaagagtt
      721 ctctgcctat gcagcacagt tagcattcgg catcaaagca ttggaaaaca ccctgccaca
      781 cctgcgtgag ctggcactcg gcggcacagc agtcggcacc ggcttgaata ccccgcaagg
      841 ttacgatgtg aaagtggcgg aatacattgc gaaattcacc ggtttaccgt tcgttaccgc
      901 ccaaaacaaa tttgaggcat tagcgactca cgatgcgatt gtcgaaaccc acggtgcatt
      961 aaaacagctt gcggtgtcgc tctacaaaat tgccaacgat gtccgtttat tagcctccgg
     1021 tcctcgttca ggcattggcg aaattctgat tccggaaaac gagccgggtt catcaattat
     1081 gccgggtaaa gtaaacccaa cccaatgcga agcaatgaca atggtatgtg cccaagtatt
     1141 aggcaacgat gtaaccatct cttttgccgg cacgcaaggg catttccaac tgaatgtcta
     1201 taaaccggtg atggcgtaca acttcctgca atcggctcaa ctgctaggcg atgcgtgcgt
     1261 gtcgtttgac gaacactgtg cagtcggcat tgaaccgaac taccctcgca ttaaacaaca
     1321 gctcgaccaa tcgctgatgt tagtgaccgc attaaatact cacattggtt atgaaaatgc
     1381 agcgaaaatc gccaaaactg cccacaaaaa cggcacaaca ctgaaagaag aggcgattaa
     1441 cttaggctta gtcacggcag aacagttcga tgaatgggtt cgtccggaag atatggttgg
     1501 cagcttgaaa taagcccaac ccttctcttg taatctgatt tacacctcaa tctaccggta
     1561 agattgaggt gtaatttttt atagcgaggg tactgatttt gttttagaat gaagcatcat
     1621 tttttaagcg gaaaaaccta tgaaaaaact cattttaatc gcattagtta ctactttagg
     1681 cttaagtgct tgtggggtca aaggcccact ctacttccct gagcaagccc ctaaacatga
     1741 acaaaaataa gcattgcaag cggtctgttt tggctaaaat tttgcaaatt tttgtgaaaa
     1801 aatgaccgct tgttgcctct tttctttcaa aatcccaaac acaacggact ttcaatgaac
     1861 cattttgaat ataaaaacaa ccaacttttt gctgaagaag tcgctattgc cgatattatc
     1921 gagcaatacg gcacgccagc ttatgtttac tccagatcta cccttgaacg ccactggcac
     1981 gcattcaata atgcgtttgg ctcgcaccca catttagttt gctttgcagt caaatccaac
     2041 tctaacattg ccttgttaaa tgtaatggct cgtctcggtt caggctttga tattgtctct
     2101 caaggcgagt tggagcgtgt cttagctgcc ggcggcgaag cctctaaagt tgtcttttcc
     2161 ggtgtggcaa aatcgcacag cgaaatcaaa cgagcattgg aagtcgggat tcgttgcttt
     2221 aacattgaat cggtttccga acttcaccgc attaaccaag ttgccggtga actaggcaaa
     2281 atcgcaccga tttcactgcg ggtaaacccg gatgtggacg cccacactca cccatacatt
     2341 tccaccggtt taaaagaaaa caaattcggc gtgagcgtta ctcaagcccg tgaagtgtat
     2401 cgcttagcca aaaccttacc gaatgtgaaa attaccggta tggattgtca catcggttcg
     2461 caattaaccg aattgcaacc ttttttagat gcaacggatc gcttaatcat cttaatggag
     2521 caactgcgtg aagacggcat tgagctacac cacttagact taggtggcgg tttaggcgtg
     2581 ccttacaacg gcgaagagcc tccgcatccg accgaatatg ccaaagcctt gctggaaaag
     2641 ctcaaaggct atgaaaactt agaaatcatt attgaaccgg ggcgtgccat taccgcaaat
     2701 gccggtattt tagtgacgaa ggtggaatac ttgaaacaaa atgaagatcg taattttgcg
     2761 attgtcgata ccggtatgaa cgatatgatt cgcccggctc tgtatgaagc ctatatgcaa
     2821 attacggaag tggataaaac cttaaaccgt gacaaagctg tttacgatgt ggtcggtccg
     2881 atttgcgaaa cctcggattt cttaggcaaa caaagagaat tggcaatttc tgaaggggat
     2941 ttaatcgcta ttcgttctgc cggtgcttat ggggcggcga tgtcttcaac ctacaactct
     3001 cgcccacaag cggcagaaat tatggttgat ggcaaccaag cccatttaat taaagcccga
     3061 gccacttttg ctgatttatg gcgtttagaa aaattattac cttaatgagg aagaaaagat
     3121 gaaattatat ggattaaccg gtgcttgttc atttgtacca cacgttgccc tagagtgggt
     3181 gaaattgcgt gccaatcaag attatgcgtt tcaagctgtt agccgtgaat ttattaaatc
     3241 ggcggaatat ctggctttaa accctcgtgg taacgtacca ttgttggttg atggcgactt
     3301 ggccctcacg caaaaccaag ctatcgtcca ctatttagac gagctttatc cggaagctaa
     3361 attattcggc agcaaaaccg ctcgtgataa agccaaagcg gctcgttggt tagctttttt
     3421 caattcagat gtgcataaat cattcgtgcc actgttccgc ttgccaagtt atgcggaagg
     3481 caatgaaacg ctcaccaaaa ctattcgcca acaatcggct gagcaaattt tagaacaact
     3541 ggcttttgcg aatgctcatt tagaaaatca tattttcttt ggcgaagaaa tttcggttgc
     3601 cgatgcgtat ctctacatta tgctcaactg gtgtcgccta ctagggttgg atttctctca
     3661 tctttcccaa ctttctgcct ttatgcaacg agtggaggca gaccaaggcg tagataatgt
     3721 gcgtgagcaa gaaggcttaa aaggttaatt caccattgtt catttaagac ggtagatttt
     3781 atgttaaaat ctaccgtctt ttatttttag gaatcaccat gctaaatcag gtttcaaaca
     3841 gcttgcttgc tccaagtggg cttgaattat cgcacttaac tcaagtgtta gatcacttct
     3901 ccggccgtca tattgattat gccgatctct acttccaact cagccaagac gaaagctgga
     3961 gcttggaaga cagcatcatc aaagaaggcg gtttttatat cgaccgaggg ttcggtgtgc
     4021 gagccatatc aggcgaaaaa accggctttg cctacgccga tcaaatcacg ttaaaacagc
     4081 ttgagcaatg tgccgctgcc gcacgttcaa tcagcccaac aagcggtcaa ttatcggtaa
     4141 aaaatttcaa aaaaacgacc gcttgttctc gctacccggc aatcaacccg ctagagacca
     4201 tcagccgtga acaaaaggtc gaattgctcc atttggtgga taaaaccgct cgaatggcag
     4261 atccgagagt gattcaagtg aatgcgaatc tctcagcggt atatgaagaa atgttagtgg
     4321 cagcgactga cggcacactg gctgcggata ttcgcccgtt agtgcgttta tctgtgtcgg
     4381 tactggtgga acaaaatggc aagcgtgaac gaggttcagc aggtgccggt ggtcgctttg
     4441 gtttagattg gttcttagca ccaagcgaaa ccggctttag ccgtgcggaa tcgcttacca
     4501 aagaagccgt tcgccaagcc ctcgttaatc tcacagccat tcctgcaccg gcaggcacga
     4561 tgccgattgt tctcggtgct ggctggccgg gcattttgct ccacgaagca gtcggacacg
     4621 gcttggaggg tgattttaac cgtaaggaat cttcgctctt cagtggtaaa atcgggcaaa
     4681 tggtcacctc tgagctttgc accattgttg atgacggtac ggtgccgaat atgcgaggct
     4741 caatcacggt agatgacgaa ggcgtgcctt cgcaacgtaa tgtgttaatt gaaaacggca
     4801 ttctaaaagg ctatatgcaa gacaaactca atgcccgcct gatgggagtt gccccaaccg
     4861 gcaacggcag acgggaatct tacgctcatt tgccgatgcc acgaatgacc aacacctacc
     4921 tcaccgaagg caatcacgaa tttgaagaga tgattgaatc ggtcgaatac ggtttatatg
     4981 ctcctcactt cagtggtgga caagtggata ttacctccgg caaatttgtg ttctccaccg
     5041 ccgaagccta cttaatcgaa aaaggcaaaa tcaccaaacc tgttaccggt gcaaccctga
     5101 tcggcagcgg catagaagca atgcaacaag tttcaatggt cggcaaaaaa atggaactcg
     5161 accccggcat cggcacttgt ggcaaagaag ggcaatccgt accagtcggc gtagggcagc
     5221 ctacagtgaa gttggataaa attacggtgg ggggaagagg gtaatctctc cccgtttgca
     5281 aaatataaag aaaatatcac cgcttgtagc gataaaagga aaacaaaatg gcttgggtac
     5341 aaattcgttt aaacagtaca gataaacagg ctgaggaaat cagcgatttt ttaaccgaga
     5401 ttggagcggt gtcagtgact tttatggata gtcaagatac cccaattttt gaacctcttc
     5461 cgggggaaac ccgtctttgg ggaaataccg atgtggttgg gcttttcgat gcggaaactg
     5521 atatgaatct tatcgtagaa gcactaaaaa ccagcaaatt ggtggacgaa aactttgctc
     5581 acaaaatcga gcaaatcgaa gacaaagact gggaacggga gtggatggat aatttccacc
     5641 caatgcagtt tggcaaacgg ttatggatct gcccaagctg gcgtgaagta ccagacccga
     5701 atgcagtgaa tgttatgtta gaccctggtt tagcatttgg caccggcaca cacccaacga
     5761 cctcgctttg cttacaatgg ttagatagct tagatttaga aggcaaaacg gttatcgact
     5821 tcggctgtgg ctccggtatt ttagctatcg ctgcacttaa actcggtgca aaacaagcca
     5881 tcgggattga tatcgaccca caggcgattt tagctagtca aaacaatgct gaggcgaacg
     5941 gcgtagcaga aaaactacaa ctattcttag ccaaagatca gccaagagag ttacaagccg
     6001 atgtcgttgt tgcgaatatt ctagccggcc cactcaaaga gttagcaccg aatatcatca
     6061 cgctggttaa accaaacggc aatttaggct tatccggcat tcttgctact caggctgaaa
     6121 gtgtgtgtga ggcttatagc gaagccttta cgctagaccc tgttgtagaa aaagaagaat
     6181 ggtgtcgtat tacaggccgc aaaatctaat ataagaagcc ctgttttaaa cagggcttac
     6241 tttttgataa attctactac tctttacctg tcttgggaat cgttctccct aaattaatta
     6301 acacaagcgg tagaaataac aggaaaattt acaaattaca taaatgaaaa aagccgactg
     6361 aaatcagtcg gctttctaaa taatggcagg ggcggagagg ctcgaactcc caacacccgg
     6421 ttttggagac cggtgctcta ccaattgaac tacgccccta ttgataggta ttcattttgc
     6481 acttataggg gcgaattact attcgcccct gcattaaaga ttattggcgg aacggacggg
     6541 actcgaaccc gcgaccccct gcgtgacagg caggtattct aaccagctga actaccgctc
     6601 ctaaattaat agattgataa tgccctactc tcacatggcg aatcaccaca ctaccatcgg
     6661 cgttactgcg ttttacttct gagttcggta tggagtcagg tagagccaca gcactctggt
     6721 tatcaatcaa attctttcat ttagtcagtt tactaaaata tagtttatac tttctaataa
     6781 actcactaa
//
LOCUS       DANLXQ010000137         6632 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.137, whole genome shotgun sequence.
ACCESSION   DANLXQ010000137 DANLXQ010000000
VERSION     DANLXQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.137"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(73..1764)
                     /gene="dld"
                     /locus_tag="PXD94_002092"
     CDS             complement(73..1764)
                     /gene="dld"
                     /locus_tag="PXD94_002092"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDL6286917.1"
                     /translation="MSNNSLISQLTEIVGQSYIITDPTKTEAYRSGYRFGTGSAIAVV
                     KPTTLLEYWNVLKACVAADVIVISQAANTGLTGGSTPSGNDYDRDIVIINTMRMNGIQ
                     LINNANQVVCLPGSTLNELELLLKTHGREPHSVIGSSCIGASVIGGICNNSGGALVQR
                     GPAYTEMALFAQLNEDGTLELKNHLGIDLGDTPEEILENLQAQRYQRKDIQQACGKGH
                     DHAYCNHVRQIDEDSPARFNADPARHYEAAGSAGKLAVFAVRLDTFPLEKDTAVFYIG
                     TNHTDTLNDIRRYMLANFEVLPISGEYIHRDAFDIAADYGKDTFWVIKKLGTHRLPQL
                     FSLKAKVDRMAKKLTFLPANLSDKILQAISKMLPQHLPESLRDYRNQYEHHLIIKMGG
                     KGVQEARDYLARYFADTQKGGYLECNPTESQAAMLHRFAVASAAIRYRAIHDKEVEDI
                     VALDIALRRNDKAWFEVLPPEIESQITHKLYYGHFMCHVFHQDYIVKKGVDCLALEHQ
                     MLELLDKRGAQYPAEHNVGHLYEAKPTLRKFYRQLDPTNSFNPGIGQTSKKKYWK"
     gene            complement(1976..3073)
                     /locus_tag="PXD94_002093"
     CDS             complement(1976..3073)
                     /locus_tag="PXD94_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL6286918.1"
                     /translation="MFYSSNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTFYRYQQ
                     AVEQGGVEALLNQNRRVPNIKNRVDESTEKAVVQFALDNPAFGQVRVSNELRKQGIFV
                     SAGGFCLCRWFLSLQVVFVSAGGVRSIWLRHHLANFKQRLTALEKLVAEQGIILSEPQ
                     IQALERKKEDEIACGEIETAHPGYLGSQDTFYVGNIKGVGRIYQQTFIDTYSKVAFAK
                     LYTMKTAITAADMLNDKVLPFFESQRLPMLRVLTDRGSEYCGKVENHDYELYLAINDI
                     EHTKTKVKQPQTNGICERFHKTILQEFYQVAFRKRIYMDLEALQADLDEWLMYYNHHR
                     THQGKMCCGRTPMETLVDGKRIWAEKNLSSN"
     gene            3403..4119
                     /locus_tag="PXD94_002094"
     CDS             3403..4119
                     /locus_tag="PXD94_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL6286919.1"
                     /translation="MYLMYVDESGSTTDPTQKYFVLSGIAVFERNTHWIEKKMNVIAS
                     QFCQYSEGSDPYFYELHGSPMRSGKSEWRGIPMSERINAIQDCLKLVQSNNIRIFAAV
                     IEQGYSSGQDTITECFEQISSRFDMYLSRLHAQGNTQRGISIFDKSSTEKSIQALART
                     FKNSGHTYGKLRNFSEVPLFLDSKSSRLIQLADLVSYAIFRHYEHNDSSFYDIIRNNF
                     DYHNGRIHGLYLRNKEDIIS"
     gene            complement(4162..5080)
                     /locus_tag="PXD94_002095"
                     /pseudo
     CDS             complement(4162..5080)
                     /locus_tag="PXD94_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(5082..>6632)
                     /locus_tag="PXD94_002096"
     CDS             complement(5082..>6632)
                     /locus_tag="PXD94_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase/relaxase domain-containing protein"
                     /protein_id="HDL6286920.1"
                     /translation="VHDIGKVVVDIEITLKDGSRWFAWNGLPPQPYKFKYIKGRDYHL
                     HPVLGGFLANQLIPKSAFDWLGQYPEVISSLMYAMSGHNDKAGLLSEIVQKADQHSVT
                     LALGGDVNKLVQRPVNSLSKQLVLALRYLLEQKIKLNTPKGPADGWFTEEGLWLVSKT
                     AADNIRAYLLGQGISVPKDNPKLFDEMQSLGIVEESPAGGAIWSCRIQADSGWTPANT
                     FTLLRIKPEVAWENINDRPNYFAGKVKIVEQITEESNIETQSTLVDGTTKPAPLPSVQ
                     TGGIIASKEVEISQVETNSDNAPSSEIIINDENDLTSFVLDMFAPTESSEPSLDDISI
                     GKGQEIASMPSKQEVQDKSTTKKLKVTQSAVETPETLTLSELQLQNKENSITGDNFVK
                     WLKSGIATNKFAINKPTAKLHIVEDHLFLVTPVIFEMYLQETGQPYDKETINNLQYDF
                     QGLGIHSPKNTMTNGKPDTINFWKCDVIGPRKTSSLTGYLIKNTRLFFGDKVLLNNHC
                     LTLQKEET"
BASE COUNT         1910 a         1346 c         1246 g         2130 t
ORIGIN      
        1 ttagcaactg ttttgtccac ttggcatagt ttttgacgga tcagcaacta ttttgtctac
       61 tatgcctttt tgttacttcc agtatttttt cttcgaggtt tgcccgatcc ccggattaaa
      121 gctgttggtt ggatcaagct gccggtagaa tttgcgtaag gtcggttttg cttcgtaaag
      181 atgaccgaca ttatgctccg cagggtattg tgccccacgt ttatccagta attctaacat
      241 ctgatgttcc aacgccaaac aatctacacc ttttttcaca atatagtctt gatggaaaac
      301 atggcacata aaatgcccat aataaagttt gtgcgtgatt tgactctcta tttccggcgg
      361 tagcacttca aaccacgctt tatcatttcg gcgaagggca atatctaaag caacaatatc
      421 ttccacctct ttatcgtgaa tggctcgata gcgaatcgct gccgaagcaa cggcaaaacg
      481 atgtaacatt gccgcttggc tttcagtcgg attacactcc aaataaccgc ctttttgtgt
      541 atcagcaaaa taacgggcaa gatagtctct tgcttcttga acgccttttc cacccatttt
      601 gataattaag tgatgttcat actggttacg gtagtctcga agactttccg gcaaatgttg
      661 cggtaacatt tttgagattg cctgtaatat tttatcactg agatttgccg gtaaaaaggt
      721 gagtttcttc gccattctgt ccacttttgc tttaagtgaa aagagttgtg gtaagcggtg
      781 tgtaccgagt tttttaatca cccaaaaagt atctttaccg taatcagcgg caatatcaaa
      841 ggcatcacga tgaatatatt caccggaaat cggcaatacc tcaaagttgg ctaacatata
      901 gcgacgaata tcgtttaatg tgtccgtatg atttgtcccg atataaaaaa cagccgtgtc
      961 tttttcaagc ggaaaggtat ctaatcgcac cgcaaacacc gctaactttc ctgcactacc
     1021 ggcagcctcg tagtggcggg caggatcggc attaaaacgg gcaggggagt cttcatcaat
     1081 ttggcggacg tgattacaat aggcgtgatc gtgtcctttt ccgcacgctt gctgaatatc
     1141 cttacgctga taacgttggg cttgcagatt ttctaaaatt tcttccggcg tatcacctaa
     1201 atcaatacct aaatggtttt ttaattccag cgtgccgtct tcatttaact gggcaaacag
     1261 tgccatttca gtataagccg gcccacgttg cactaatgca ccccctgaat tattacaaat
     1321 accaccgata accgaagccc cgatacagga cgatccgatc accgaatgcg gttcacgtcc
     1381 gtgcgttttc agcaacaatt ccagctcatt taatgtcgag cccggtaagc aaacgacttg
     1441 attggcattg ttaattaact gaatgccgtt cattcgcatt gtgttaatga tcacaatatc
     1501 ccgatcataa tcgttgccgc ttggtgttga accaccggtt aatccggtat tggccgcttg
     1561 tgagataaca atgacatcgg cggcgacaca ggcttttaat acgttccagt attctaataa
     1621 cgttgtaggt tttaccactg caatcgcact acccgtgcca aagcgatacc cgctacgata
     1681 ggcttccgtc ttagtcgggt cagtgatgat ataagactgc ccgacaattt cagtaagctg
     1741 agaaatcagt gaattatttg acattatgct tccctcaaag gtagaaatag atagggcagt
     1801 atagcaataa acaaaagaga gcaaagcaga atgttacaaa attgtgaact actgcacaga
     1861 ataaatgaag aaatgaagta aaagaaagcg agctaaaaga gcgtcgcccg ctttatttgt
     1921 agaaaatcaa acctaatctg acagtccccc gtttaaaatt accgtgtctg tcagattaat
     1981 ttgagcttaa attcttttct gcccaaatgc gttttccatc aactaatgtc tccatcggtg
     2041 ttcttccaca gcacattttt ccttgatggg ttcgatgatg gttataatac attaaccatt
     2101 catctaagtc agcttgcaat gcctctaaat ccatataaat ccgttttctg aatgccactt
     2161 ggtagaactc ctgcaaaatc gttttgtgaa aacgctcaca gatgccgttt gtttgtggct
     2221 gtttcacttt ggtttttgtg tgctctatgt cattgatagc caaataaagc tcataatcgt
     2281 gattctcaac tttcccacaa tactcactgc ctcggtctgt cagcactcgt aacataggta
     2341 acctctggct ttcaaagaat ggtaatactt tgtcatttag catatcagca gcagtgatag
     2401 ccgtcttcat tgtatacagt tttgcaaatg ccactttact ataagtatca ataaacgttt
     2461 gctgataaat acgacccaca cctttaatat tgccgacata aaaggtatct tgtgacccta
     2521 aatagccggg gtgtgccgtt tcaatttcac cacaggcaat ctcatcttct ttcttccgct
     2581 ccaaggcttg tatctgtggc tcactcagaa taatgccttg ctctgccacc aatttttcga
     2641 gggcagttaa gcgttgctta aaattagcta agtgatgtct caaccaaatg gaacgtacac
     2701 cacctgcaga gacaaaaacc acctgcagag acaaaaacca cctgcagaga caaaaaccac
     2761 ctgcagagac aaaaatcccc tgtttacgca gttcattact cactcgaact tgaccaaaag
     2821 caggattatc taaagcgaat tgtacaaccg ctttttctgt ggattcatcc acacgatttt
     2881 taatgttggg aacacgtcga ttttgattta gtaatgcctc aacaccgcct tgttcgacag
     2941 cctgttgata gcgataaaaa gtatctcgac tcatccccat taccttacag gcttgtgaga
     3001 tattacccag ttcttctgct aaatttaata aaccggtctt gtgtttaata agtggattgc
     3061 tagaataaaa catgagagtt tcctttttgt ttagattgat ttttgacact catattctaa
     3121 acgggaaact ctcacttttt agagtgaatt gtcagatcaa gtctgatctt ctacagacga
     3181 tccaggtctt acacattctt tcaagtttgt aaagcctgaa aattattttt tatacttgat
     3241 ttttgtcaaa aagtctctac actatatggc agggtcagtt ttcgctgttt aacggtgttt
     3301 aatgagattt tcattactgg ctctccttaa tagaaattta tggaagagct atctgataga
     3361 gtaatctagc gatagctctt tttcattgaa caaagaggct atatgtatct tatgtatgtt
     3421 gatgagtctg gttctacgac agatcctact caaaaatatt ttgtattatc aggtatcgct
     3481 gtatttgaac gaaatactca ttggattgag aaaaaaatga atgtgatagc ctctcaattc
     3541 tgtcaatatt ctgaagggag tgatccatat ttctatgagt tacatggttc accaatgaga
     3601 tctggaaaga gtgaatggag aggcattcca atgagtgagc gtattaatgc cattcaagat
     3661 tgcttgaaat tagttcaaag taataatatt cgtatatttg cagcagttat agagcaaggg
     3721 tattctagtg gtcaggatac tattacagaa tgttttgagc aaatatcatc tcgatttgat
     3781 atgtatctga gccgtttaca tgcccaaggt aatacccaaa gaggcatttc tatctttgat
     3841 aaatcatcga ctgagaagtc aatccaagca cttgcaagaa cattcaaaaa tagtgggcat
     3901 acttatggga aacttagaaa tttctcagaa gttccattat ttcttgactc aaagtcatcg
     3961 agattaattc agctagcgga tctagtttcc tatgcgatat ttagacacta tgaacacaat
     4021 gatagctcat tttacgatat tataagaaat aactttgatt atcataacgg gcgtattcat
     4081 ggtttatatt tacggaacaa agaagatatt atttcataaa aattcgcccc aaaagggaga
     4141 agtatctccc ttttgtattt tttataacaa attaaccgtt tctctcaaca tttccacatc
     4201 gtgttcaata tatcctagtg tcacactgat atctttatgc cctaacagtt tctgaaccac
     4261 ataaacattt tcgggcttct tcattaattg agtagcaact gtatgtcgaa atctgtgtgg
     4321 tgatgaagta aaaccgacca agtcagaaat atgccgaaat atatgtgtaa gctgattttg
     4381 gctcatcggc tgtcctcttc ttcgagagca tcgagaaaat aaattgagat tgaacaattg
     4441 gcaattatct gtttgttttc ttatttttaa ttcagtaata agcctttcta tatgaatata
     4501 aagtgaatct gaaatcggta cgatatggta atcatgattt ttattgattt ccggcgaaat
     4561 ataaatcgtc ctattagaaa gatctacatc actaattttc agtttcacta attgtcctcg
     4621 ccgaatcccc gtacatctca atgtcataat aattgcaagg acaaaccagc ttggttttag
     4681 aaagtcaggt aatacaacgt tattattgag tatgtggtct attttatgta attgttcggt
     4741 tgaataagtt ttctttctcc ttttcgattc tcgaataaat aactgattaa atggatttcg
     4801 atttatatta agtaatcctt gcgatatacc aaagttatat aaggctttta aatgtctgac
     4861 ataattattc cacgttactg ctttgatttt atcgccaaca atttgattac gccattcaat
     4921 gagcatatcg gtggtaatgt cattggcatt taactttgga taacgctttt tgataattcg
     4981 gattacgttg ttgtagctcc gtttggtatc cggacgaaga tagtgacgat agaaaaaaaa
     5041 atcggtctag tagcgaatca aaagagatag aagttatcat tttaagtttc ctctttctgt
     5101 aaagttaaac agtgattatt taatagtacc ttatccccaa aaaagagacg ggtatttttt
     5161 atcaaatatc ctgttaaaga ggaagtcttt ctcggtccaa tgacatcaca tttccaaaaa
     5221 ttgatggtat ccggcttacc gttggtcatt gtgtttttag gtgaatgaat ccctaatcct
     5281 tgaaaatcat actggagatt attaattgtt tccttatcat aaggctgtcc agtctcttgc
     5341 aaatacattt caaaaataac gggggtaact aaaaacaggt ggtcttcaac aatatggagc
     5401 ttggcagtag gcttgttaat tgcaaatttg ttggtcgcaa tacctgattt gagccacttt
     5461 acaaagttat ctcctgtaat tgagttctcc ttattctgta actgtagctc tgatagtgtt
     5521 aatgtttcgg gcgtttctac tgcactttgt gttactttga gctttttagt ggtagattta
     5581 tcctgtactt cttgtttgga tggcatactt gcaatttcct gtcccttacc aattgaaata
     5641 tcatcaaggc ttggttcgga actttccgta ggtgcaaaca tatccagcac aaaggaggtt
     5701 aaatcatttt catcatttat gatgatttct gaggatggtg cattatccga gtttgtctct
     5761 acctgagata tttcaacttc tttagaggca ataatgcccc ctgtctgaac actcggtaat
     5821 ggagctggtt tagtggtccc atcgactaat gtagactgag tctctatatt actttcttct
     5881 gttatctgct cgactatttt aacttttccg gcaaaataat ttggtcgatc attaatattc
     5941 tcccaagcca cttctggttt aattctcaat aatgtaaagg tatttgctgg cgtccaacct
     6001 gaatcagctt gaatacggca actccaaatt gctccaccag ccggtgattc ttctacaata
     6061 ccgagagact gcatttcatc aaataacttt ggattatcct taggcactga aattccctgc
     6121 cccagtaagt aagctcggat attatcagca gcagttttgc taacaagcca caatccttcc
     6181 tcagtgaacc aaccgtctgc cggacctttg ggtgtattta actttatttt ctgttccagc
     6241 aaataacgta gtgccaagac cagttgcttt gacagagaat tgacaggacg ttgaaccagt
     6301 ttatttacat cgccaccgag tgcgagggta acactatgct gatcggcttt ctgcacgatc
     6361 tcagataaaa gcccggcttt atcgttatgc ccagacattg catacatcag tgaagaaatg
     6421 acttctggat actgtcccag ccaatcaaac gcagattttg gaataagttg atttgcaagg
     6481 aagcctccta gcaccggatg aagatggtag tctcgacctt tgatgtattt gaatttataa
     6541 ggttgaggtg gtaacccatt ccaagcaaac caacgtgaac cgtctttgag tgtaatttca
     6601 atatcaacga caactttgcc aatatcgtga ac
//
LOCUS       DANLXQ010000138         6543 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.138, whole genome shotgun sequence.
ACCESSION   DANLXQ010000138 DANLXQ010000000
VERSION     DANLXQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.138"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..770
                     /locus_tag="PXD94_002097"
     CDS             <1..770
                     /locus_tag="PXD94_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL6286921.1"
                     /translation="LYESYLALNTKLDFTRYIQKARKFNEYNTDILYRSFDNFFAHYD
                     TGVKHNNLSLGLNTKLSENLRGGLFFEYSNKNRNSVQIGARAKYEMDNHQLSGFLRYR
                     GVKHKDMLARNNSVDGYLNYAYRIQLDDKLSISPSVGAYVSRSSRSLIDEDLAINSRT
                     VYASDVGLNIHYKLNDIDAYIRPSIGFVNGDITLSQSNDMTNTYKIKDKSNVYSVTTG
                     LKKRFANGVALGADFKLHRYGSQTTESNFGLNVSYNW"
     gene            complement(937..1473)
                     /gene="hpt"
                     /locus_tag="PXD94_002098"
     CDS             complement(937..1473)
                     /gene="hpt"
                     /locus_tag="PXD94_002098"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL6286922.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQKYRHLDYIAKVVIE"
     gene            complement(1512..2861)
                     /gene="pmbA"
                     /locus_tag="PXD94_002099"
     CDS             complement(1512..2861)
                     /gene="pmbA"
                     /locus_tag="PXD94_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL6286923.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLGKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTIFREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMSVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPEWFAISERPHLLRQLASSAFDSEGVITQNREIITDGVLQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRQGGLNTLLKEMGTGLLVTEMLGSAINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            2960..3490
                     /locus_tag="PXD94_002100"
     CDS             2960..3490
                     /locus_tag="PXD94_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL6286924.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKSEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            3496..3867
                     /locus_tag="PXD94_002101"
     CDS             3496..3867
                     /locus_tag="PXD94_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL6286925.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            3880..4593
                     /locus_tag="PXD94_002102"
     CDS             3880..4593
                     /locus_tag="PXD94_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL6286926.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLPLVFGLCCFGL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVAGLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLIIVQGILILLATVIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            4596..5159
                     /locus_tag="PXD94_002103"
     CDS             4596..5159
                     /locus_tag="PXD94_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL6286927.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVISKGAELT
                     RTLETDYTREALHDLADQIHSLNNVGASIASGSFKTEGMVIAPCSMRTLAAIANGLSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQKPTSL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            5222..5662
                     /locus_tag="PXD94_002104"
     CDS             5222..5662
                     /locus_tag="PXD94_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL6286928.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENVR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(5666..6541)
                     /locus_tag="PXD94_002105"
     CDS             complement(5666..6541)
                     /locus_tag="PXD94_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6286929.1"
                     /translation="MMKTILIDEANQIKGFSHLYRLRSDLMLKYTEISVTETVEQEPA
                     EFEAGLRLVILLSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFRKGQLQKE
                     LVIFLYPKWLIESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEMQPFGLLE
                     KEGNLLALLASAFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYFHTNVTTL
                     QNEFQHYHKTSIFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFSTAFKRTFG
                     ITPSQIKRDKAKGII"
BASE COUNT         1933 a         1335 c         1214 g         2061 t
ORIGIN      
        1 atttatatga aagctatttg gctttaaata ctaaattaga cttcactcgc tacatccaaa
       61 aagcgagaaa gttcaatgaa tataacactg atattttata tcgtagcttt gataatttct
      121 ttgcccatta cgacacaggt gtaaaacaca ataatctatc cttaggcttg aatacaaaat
      181 taagtgagaa tttacgtggt ggcttattct ttgaatacag taataaaaat cgtaatagcg
      241 tacaaattgg tgcaagagct aaatatgaaa tggataatca ccaactttct ggcttcttac
      301 gctatcgtgg cgtaaaacat aaagatatgc tagcacgtaa taacagtgtt gatggctacc
      361 taaattatgc ttatcgtatt caattagatg ataaattaag cattagccca tcagttggtg
      421 cttacgtttc acgttcttct cgctcattaa tagatgaaga tcttgcgatt aatagccgca
      481 cggtctatgc aagtgatgta ggattgaata tccattataa gctaaatgat attgatgctt
      541 atattcgtcc aagtataggc tttgtcaatg gtgatataac tttatctcaa tctaatgata
      601 tgacaaacac atacaaaata aaggataaaa gtaacgtgta ttctgtcacg acagggttga
      661 aaaaacgctt tgcaaatggt gtggcattag gggctgattt caaattacat cgttatggtt
      721 ctcaaactac tgaaagtaat ttcggtctaa atgtaagtta taactggtaa taccgtaaac
      781 tgattacgat ttaaaagtta gactagccca agactgagtt ctatattcca tagagctcag
      841 tcttttaatt tttctttctc gataagaata ttttatattt caaccttaat cgagaatgta
      901 attagtcgtg aaaatctaag tagtgattga gtttttttat tcaatcacta ctttagcaat
      961 ataatctaaa tgacgatatt tttgggcata atcaatccca taacccacca caaattcatc
     1021 ggggatctca aatcccaccc attttacggg cacatcaact tcgcggcggc aaggtttatc
     1081 taataaagta caaatagcaa gtgaatttgg ctcacgtaac ttgagaatct cccctacctt
     1141 gcttaaggtg aagccagtat caataatatc ttctacaatc aatacatctt taccttgaat
     1201 ttcgccatct aaatctttta gaattttgac atcacggctg gattccgtgc tgctgccata
     1261 gcttgatgcg gttagaaaat ccacttctac aggtaactct agctttctta ctaaatctgc
     1321 cataaacata aacgagcctc gcaataaacc aaccactaca aggttttggc actgtttttg
     1381 gtgatagtgt tgattgattt cttttgctaa ttcattgatt cggtttaata cttcttcttt
     1441 tgaatgtagc gtttcgatat gatgtttttc cattgatttc tccaaataaa attgattaca
     1501 agcggccgtt tttactcgcc tgaaaccatc attttttcta ataagattga acctgtttgg
     1561 atatttgagc ggtgttcaat atcatcgcct accgccacta aatttttata catatcttgc
     1621 agttgccctg caatggtaat ttccgctaca gggtattgaa tttcaccgtt ttctacccaa
     1681 aatcctgctg cacctcgaga gtattcccct gttacgctat taatcgcaga acctagcatt
     1741 tccgttacta ataatcctgt tcccatttcc ttaagtaacg tatttaaacc accttgacga
     1801 tttggtttaa ctaaccagtt atgaatacca cctgcgtgtc ctgtactttt taaccccatt
     1861 ttacgccctg aatagctagt cattaaatac gtttgtagca cgccatcagt aataatttca
     1921 cggttttggg taattacacc ttcactatca aacgcagaag aggctaattg acgcaacaaa
     1981 tgcggacgct ctgaaatcgc aaaccattca ggcagaatct gttcgcctaa tttatctaat
     2041 aagaaacttg atttgcggta taatgcaccg ccactaatag ctccggccaa atgccccact
     2101 aaaccggttg ccacatcgtt atagaaaatc acagacattt cgccggtctt aattcgttgc
     2161 ggattcaatc tatctaccgc tttaattgca gcttgctccc ctacccattt cggtgcttgt
     2221 aatttatcaa actcacggaa aatggtgtat tcataatcac gctcaagctg atcttcataa
     2281 gctgaaatca cgctacaaga gagtgaatat cggctcgaca ggtaactctg caacattccg
     2341 tgcgtattgc cataaacacg cacgccactg tgagaattga aagttgcccc gtcactattc
     2401 acaatttttt cgtctgcatt taaggcataa tgctccgctt caatcgccaa ttcgactgct
     2461 tgatcaactg aaatatccgc ttggtggtac aattccaaat cttctgcctc aaaggcaagc
     2521 aattctttat cagcaagccc ggtacaagca tcttctgagg tatattttgc aatcgcaaga
     2581 gctgcttcaa ccgttttttg aatcgactct ggctgaagat cggaggtgga agcattacct
     2641 ttgcgtttgc ctaaataaac cgaaataccg agagaaccat cgttattgaa ctcgatgttt
     2701 tcaacttgct ctaaacggct tgaaaccgaa agcccggcaa ctttagtcac gcctacttcc
     2761 gcctctgccc cagcctgttt tgcaaaattt aaggcaaatt cgaccgcttg tcgtagctcc
     2821 tgttcttgtt gtaacaaggt ttgtttatct gtaattgaca ttttgttcca ttgattgatt
     2881 gcattgatga atatattcta actcaaattt ctgttagaat acggcatttt ttagagttta
     2941 tgtaatgaaa ggataaagaa tggctaaaaa acggagcaaa aacgaaattg actggacaga
     3001 tgaggaagaa gaaatcatct gggtcagcaa aagtgagata aaacgagatt ctgaacactt
     3061 aaaaaaaatt ggggctgaat tgatcgaatt gaccccacaa aatttggaaa aaattccact
     3121 agacgatgat ttaaaagagg caattcgcca agctcaaggc tttaaaatgg aggctcgccg
     3181 ccgtcaaatt caatttatcg gcaaattatt acgcaaccgt gaccaggagc cgattcaaga
     3241 agccttagat aaagtgaaaa atcgccataa tcagcaacaa gccctattac ataaattgga
     3301 attagtgcgt aatcaactga ttgcaatggg cgatgcatca ttagataatt tattgacgga
     3361 atatcctcaa ttagaccgcc aacatttgcg taatttaatt cgtggggcaa ttaaagagcg
     3421 tgaagccaat aaaccggcaa aaaattaccg tgaaatttat caatatttaa aaactgaaat
     3481 cgtggagtga ttattatgga gtacctatca accattctta aagctcatat cggcttagct
     3541 tatattagtt taattttatt attaacccgt ggatttttat cgtcaaagat ggtggattgg
     3601 cgacaatata aaattttgcg cattgcccca cacgcagttg ataccttctt acttatcacc
     3661 ggtattgctg ctgtcgctat tttcttagct tatgattttt tcaccttagc acaattcagt
     3721 tggttgctac caaagttact cttcttagta ctttatattg tatttgcgac aaaagcgttc
     3781 aaaaaaggac aaccgttttc gctcaaattt tatctgttag ccgtagtctc attcttatta
     3841 atgatgctaa ctgccacttt tcactaaata agggaaacta tgatcgggcc ttttgtgaat
     3901 gccggtgcaa ttattttagg gggcgtgctg ggtgcttgtt tagggagtca cctgcctgaa
     3961 cgtattcgca ctacgttgcc gctggtattc ggcttatgct gtttcggctt aggtatgatg
     4021 ctgactgcct cagtaaaata tatgtctgcg gttgccttag cattgatttt aggcacgatc
     4081 atcggtgaac tgatttattt agaaaacaga atcggtaaag ttgccggttt agctcgtggt
     4141 ttaattgacc gcctattccc accggtgcat agtctttccc aagaagagtt tttagagaaa
     4201 tttattgctc tgcttgtact gttttgtgtg agcggtaccg gcatttttgg tgcaataaag
     4261 gaaggaatga caggcgatcc aagtattctt tacattaaat cgattctaga tttcttcacc
     4321 gcaatgattt tcgctgcttc gttaggctat gccattgcgg cgattgcagt gccgttgatt
     4381 attgtgcaag ggattttaat tctactggca accgtgatta tgcctttaac cactgccaat
     4441 atgatggcag atttctccgc aaccggcggt ttactgctat ttgcaaccgg ctttcgcatt
     4501 tgtggaatta aaaactttcc ggtcgcaaat atgctaccag cactactcct tgcgatgccg
     4561 atgtcttatt tctgggaatg gttggtacat taatgatgaa aaaacgtatt attgtcggca
     4621 ttagtggggc aagtggtttt caatatggct ataaagtgct tgaactgctg aaaccgtttt
     4681 cagaaattga aacccacctt gtaatcagca aaggagctga actcactcgt actctcgaaa
     4741 cggattatac ccgagaagct cttcatgatt tagctgatca aatacattcg ctaaacaatg
     4801 tcggagcaag cattgccagt ggttcattta aaaccgaagg tatggtcatc gccccgtgtt
     4861 ctatgcgaac ccttgccgct attgccaacg gtttgagcga taatttgctg acccgtgcgg
     4921 cagatgtggt gttgaaagaa cggcgtaaat tggtattaat ggtgagagaa gctccgctca
     4981 accttgctca tattgacaat atgcgaaaag tcacagaaat gggcggcatt attttcccac
     5041 ccattccggc gttttaccaa aagccaacca gccttgacga gatggtcacc cacagcgtaa
     5101 gccatgccct ttccctgttg gatattgaca ttccaaatac cccacattgg ggagaataat
     5161 tcggcttatc ttttgctttt tgaaatgcta gaatacctga taaaaactat caggtatttt
     5221 tatgcacaat ccaatcccga cccatatttt agatttcatt caaactaacc acgttgtcaa
     5281 tattgcagct cataccgaac aggatttttg ggcagcaaat tgcttttatg cctttgacca
     5341 agaaaatgtt cgcttgatta tcttaaccgc caagaaaacc agacacgctg gcttgatgct
     5401 tgaaaaccca catattgtcg gcacaatttg tgggcaaatt gagaagatca acgaaattga
     5461 aggtgtccaa ttctccgcta ccgccgaatg tttagaagat aaacaagccg aaaaagccgc
     5521 tttgcaaatt tattaccaaa aacacccgct tgcacggctt aagcctagcg atgtttgggt
     5581 gttatcattc aataccatca agcacaccgg taataaactt atgtttgcga aaaagacgat
     5641 ttgggagaga aatataattt gaagctcaaa taatgccttt agccttatcc cgtttgattt
     5701 ggcttggtgt tatcccaaaa gttcgcttaa atgccgttga aaaattctcc gaattggcat
     5761 atccggcaaa ttcagctgct tgtgtaatac tctgcccttg taatagtaat ttataggctt
     5821 gttctaattt agaacgcctt aaatacgcaa aaatagaggt tttgtggtaa tgctgaaatt
     5881 cattttgtaa tgtggtcaca ttggtatgga aatatattgc aatctgcttc aaactccagc
     5941 cgtcaaattt accgctctca agcaattctg ttaactgttg cacccgatct ttttttaacc
     6001 ggggagcctg tgtcagctga gaaaaagcac ttgctaataa agctaataga ttaccttctt
     6061 tctcaagtaa cccaaatggt tgcatttcct gctctgacac aatatcgtga ataagtttta
     6121 acatactctg agtcaaaata atttggtggg cttttaaatg ctgtttaaag tgatgatagc
     6181 ttgccaaatt taactggctt tctattagcc atttagggta gagaaagata acaagctctt
     6241 tttgaagttg ccctttccta aaaattttac gccccatttc agctctattt agcggtaaaa
     6301 atgcggcttg aggttttaaa cttgcatcta attcaaatac ttccgaatta aaatcaattt
     6361 ccgatcgacc actcaacaat ataaccaatc ttagtccggc ttcaaattcc gcaggttctt
     6421 gctctacggt ttcagtaact gaaatctctg tatatttcag cataagatct gaacgtagtc
     6481 ggtataaatg tgaaaaacct tttatttggt tagcttcatc aatcaagatt gttttcatca
     6541 tta
//
LOCUS       DANLXQ010000139         6461 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.139, whole genome shotgun sequence.
ACCESSION   DANLXQ010000139 DANLXQ010000000
VERSION     DANLXQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.139"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..707)
                     /locus_tag="PXD94_002106"
     CDS             complement(<1..707)
                     /locus_tag="PXD94_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6286930.1"
                     /translation="MSIYRRKANGPWWVDITTPNGKRIKRSTRTLVKREAQQYHDKLK
                     QEMWAEDKLEEKRKYIFEDALLHYVRAAEELKDKATKKRHAEYWLSKFAGRELSSLTA
                     QEIILNIPKKNANTKKPLSHSTQNKYVKSLSRVLNIAHKLGMLDAVPHIPKKKEPPIR
                     VRWITKEQAKQLIDKLSSDWMKSICKFALMTGARRTEILTMTWDKIDLVRKVAIVTND
                     VAKSGKARSLLLNQEAI"
     gene            complement(611..934)
                     /locus_tag="PXD94_002107"
     CDS             complement(611..934)
                     /locus_tag="PXD94_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6286931.1"
                     /translation="MEHLTINQVAKELNCHPNTVRQHLADWGFFKMVGSEVWRINRED
                     LERAKKRAGIDPIRKRSDNDRGLSLSVEENEKCRSTDEKQTVRGGLISQPQTARELRE
                     ALARL"
     gene            complement(1015..1182)
                     /locus_tag="PXD94_002108"
     CDS             complement(1015..1182)
                     /locus_tag="PXD94_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL6286932.1"
                     /translation="MQFTYGSICSGIEATSIAWHGLGKPLWFSEIEPFPCAVLAHRFP
                     NVPNGNGMAVP"
     gene            complement(1190..1564)
                     /locus_tag="PXD94_002109"
     CDS             complement(1190..1564)
                     /locus_tag="PXD94_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286933.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEECQIRCPKTYAKL
                     REILEANEENVVRIGNLSISIG"
     gene            1714..1941
                     /locus_tag="PXD94_002110"
                     /pseudo
     CDS             1714..1941
                     /locus_tag="PXD94_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1966..2361
                     /locus_tag="PXD94_002111"
     CDS             1966..2361
                     /locus_tag="PXD94_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6286934.1"
                     /translation="MKIHTQIRKEILSLLEAKLTDIEHFYNGQPNFIDIDEQQLAISV
                     YLDEINRQELTLCDEQWTAQLNITIYLKSVDEAEDELDEWAEKIREVVETYSAFEHLE
                     GISLSQYQYEQDQNQRTWHSATLIFDVEY"
     gene            2393..3034
                     /locus_tag="PXD94_002112"
     CDS             2393..3034
                     /locus_tag="PXD94_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286935.1"
                     /translation="MAKTTKVQGTKFRIGIGREAQKAITAITLATATLTIATSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGDQVKLCEEVNWTGKDLPANYTKAKAALVQYSDQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPSTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            3117..3518
                     /locus_tag="PXD94_002113"
     CDS             3117..3518
                     /locus_tag="PXD94_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286936.1"
                     /translation="MTLREKLLANKPKLQSIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            3563..3793
                     /locus_tag="PXD94_002114"
     CDS             3563..3793
                     /locus_tag="PXD94_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL6286937.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRAAQLAHLTAMINRDPKGK
                     APELSEFMPFFNRADEVEANEDDGVADYLAKR"
     gene            3861..4127
                     /locus_tag="PXD94_002115"
     CDS             3861..4127
                     /locus_tag="PXD94_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL6286938.1"
                     /translation="MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAK
                     TKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEAFHKAMLAE"
     gene            4131..4400
                     /locus_tag="PXD94_002116"
     CDS             4131..4400
                     /locus_tag="PXD94_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL6286939.1"
                     /translation="MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKY
                     RDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVRLGSHAELFG"
     gene            4475..4738
                     /locus_tag="PXD94_002117"
     CDS             4475..4738
                     /locus_tag="PXD94_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286940.1"
                     /translation="MKNVLLFIVHFFLIAMLSIIPILIILGAIERDPYYTAWIFLSVF
                     LGIGALLYFATIVSKLINKGKTQTLEEKRYAEYWNNVKVNIPK"
     gene            4798..5508
                     /locus_tag="PXD94_002118"
     CDS             4798..5508
                     /locus_tag="PXD94_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL6286941.1"
                     /translation="MSGLGKLTVTLELENAKFQSAMTKSDYEAQKFAKNFIQNMDRAR
                     NHAKEFADRSTQYLKNIEQAAKNLNKNSEFSFLSTIGGHYQSMSSGILSAAKAYTDIQ
                     NKMKLVSGSSAEAAKRLTDVFDIATKTSQSTEAVSGVYQTFAQNAQSLGLAQKDVAEL
                     TKTVSQAVAASGASSSAASNALTQFGQSLLMGKMKAQEFNSLITQTPTIIQAMAKGLG
                     MTMAEFKAAVYRAKSLLG"
     gene            5486..5776
                     /locus_tag="PXD94_002119"
                     /pseudo
     CDS             5486..5776
                     /locus_tag="PXD94_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            5766..>6461
                     /locus_tag="PXD94_002120"
     CDS             5766..>6461
                     /locus_tag="PXD94_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL6286942.1"
                     /translation="MKFSKYQLLVNKICFICGKKDCPQMKKSKDYKDFLDALEKGDVD
                     KADKIYNTKFSQFSKSFGHEMEKNLEKNRIPPIYQGVPFDSFDLYQHYSYGNGEALNL
                     SSVGLSNKIRPLVNKDKAFGKSNGSIESRFISQYKNEGRTSFSNAYDFTKEASGIADP
                     LWALGTATISGELKDIQIFPGGIARGNIHYSIRDRFTDPFDTFNFTKGEWNPNGTPYI
                     IQDSWIKSVRFSID"
BASE COUNT         2051 a         1276 c         1248 g         1886 t
ORIGIN      
        1 ttaattgcct cttgattcag taatagcgat cgtgccttac ctgatttagc gacatcatta
       61 gtcacgattg ctacctttct tacaagatct atcttatccc aagtcattgt caaaatttct
      121 gtccgtctgg ctccagtcat caaagcaaac ttacagatag atttcatcca gtctgaactt
      181 aatttatcta ttagctgttt tgcttgttct ttggtgatcc agcgtacacg aattggcggc
      241 tctttctttt tcggtatgtg tgggactgca tccaacatac caagtttatg agcgatattt
      301 aatactctcg ataaagattt cacatactta ttttgcgtac tatgagatag aggctttttc
      361 gtatttgcat tctttttcgg gatatttagg ataatctcct gagctgttaa agagctgagt
      421 tcacgaccgg caaatttgga taaccagtat tcagcatgtc gcttttttgt ggctttatct
      481 tttaattctt cggccgcacg aacgtaatgg agcaaagcat cttcaaagat atattttctc
      541 ttttcctcga gtttatcctc tgcccacatt tcttgcttga gtttatcgtg atactgctga
      601 gcttcacgtt ttacaagcgt gcgagtgctt ctcttaattc tcttgccgtt tggggttgtg
      661 atatcaaccc accacggacc gtttgctttt cgtctgtaga tcgacatttt tcattctcct
      721 cgaccgacag agataaccct cggtcattat cagatcgttt tcttattggg tcaataccgg
      781 ctctcttttt tgctctttcc aaatcttctc ggttgatccg ccaaacttcc gagcctacca
      841 ttttaaaaaa tccccaatcc gccaaatgtt ggcggactgt atttggatgg caatttaatt
      901 cttttgccac ttgattaata gtaagatgtt ccattcatta ctttctccaa aataaatccc
      961 tctaaacgag ggattgtact aaataatcgt taagtctttg ccttatccac tgcatcaagg
     1021 cacagccata ccgtttccat ttggcacatt ggggaaacgg tgagctaata ccgcacaggg
     1081 gaatggctca atttcagaga accacaaagg cttacctaaa ccgtgccacg ctatacttgt
     1141 agcttcgata ccgctacaaa ttgagccgta ggtgaattgc attttttacc tatcctatcg
     1201 aaatactcaa attcccaata cggacaacat tttcttcgtt ggcttctagg atttctctca
     1261 acttagcata ggttttgggg catcttattt gacattcctc ttcgttgtag agagcctcta
     1321 atgtgtgcca ttcttttgca agtgcagccc aaactttccc aagttcagcc attttttcaa
     1381 tcctatttcg caaaaaaggg actgtctcca acaacagaaa gcaacgcctg aaatccgata
     1441 catcgtgtgg gtagccttct atttttggtc ttattccgta accaaggtaa aaagccattg
     1501 ttttactgct tacccctgtc tctccattgg caagccacca agcgattttg tcttctatgt
     1561 gcatttttcc tccaaaaatt aaccgcttgc ggaacaagtt ctacaagcgg ttgtcaagtg
     1621 ttcatttgtt caatgaacag ttcattgtga ataacaaaac cgcttgaatt acaagcggtc
     1681 tattttgttg cgtaaattta ctgttagcgt tgagccataa ccgactacga ccgctggccc
     1741 gaagatattg acggcgaggg ggacggcttc tcgcttgcct ccaaacggac gaccactttt
     1801 atgtcagccg gaatgacttt agtggagagt tcgcctggta aagatattgt cgatctcaaa
     1861 catatcccga aaagtagcca cgaagcccca ccgactaccg gtattttaag cctctacaat
     1921 cgaggcgacc gcagacgctt tagcgaaaca gttgataggt aagctatgaa aatccatacc
     1981 caaatccgaa aagaaatcct ttcattatta gaggcaaaat taacggacat cgagcatttt
     2041 tataacgggc aaccgaattt cattgatatt gacgaacagc aacttgccat ctctgtttat
     2101 cttgatgaga ttaaccgtca ggagcttacc ttatgcgatg aacagtggac agctcaactt
     2161 aacatcacga tttatctaaa atcagttgat gaggcagaag atgaattaga tgagtgggcc
     2221 gaaaaaatcc gtgaagtggt tgaaacatat tctgcgtttg aacatttaga gggcatcagc
     2281 ctttctcaat atcaatacga acaagatcaa aatcaaagaa cttggcactc agccacattg
     2341 atttttgatg ttgaatacta acaagcaaat aatccttata ggagaatcta caatggcaaa
     2401 aacaacaaaa gtccaaggca caaaatttag aattggcatt ggacgtgaag cccaaaaggc
     2461 aattacggct atcactttag ctacagcaac gcttactatt gccacatcag gctataaaaa
     2521 aggcgatgca atcgaaatta ccggttgtgg tcagttagac ggcatttatc ctgtattatc
     2581 tgtaacaggc gatcaagtca aattgtgcga agaagtaaac tggacgggta aagatttacc
     2641 ggctaattac accaaagcca aagcagcttt agtacaatac tcagaccaat tctgtgcggt
     2701 aaaaaacatt gaaaaatctg atgatacgtt aagtaccgaa gatgttacaa cggtctgctc
     2761 ggaaggtaca gaaaccgaac ccggagaaat tgaatttggc tcaattaaac tgagcttttt
     2821 tcacaagcca agtacggaaa tgcaaactcg cttacgcaaa ttgttttatg acaaatccac
     2881 ctttgcgtac aaattagaat tacctgataa ccacggcaca acctatggcg aaggtttcat
     2941 tgaggcgggt aacggcttta gtggcgaagt taaaggcaaa tatgaaggct cggtatcaat
     3001 taaaccaagc aaacgtgatt atttgctagt gtaattacaa aatattgctc caaatcgacc
     3061 gcttgtaata ttgcaagcgg tcttttttat cctaaatttt acaaaaggaa tcgacaatga
     3121 cactccgtga aaaactcctc gccaacaaac caaaattaca atctatcgaa atcaacggtg
     3181 agacctacta cctgcgtgaa gccactgttg gcgatatgaa taagcagatt tttgaaaccc
     3241 gaagctggct cattcaacaa gctgaacaag aaaatgttga attaccggca gaagatgatg
     3301 aaacctttga cgaagctctc aaccgttttg gcgaaaaata ccgccttgct caatcggttg
     3361 cctaccgttt atgtgacgaa aacggtgcat tactgtttaa cccacttaac attgacgatc
     3421 tcaatgcgat tgccgaatta gacagtaaag tgattatcga ctttaaccaa gccgtgtccg
     3481 ccccaaaaga ctcagcgagc gaagaaagtt ccaaataacc ctttcgctcg cactgggtaa
     3541 aacccttgaa gaaatcgaac aaatgcctga acgccacttt gccgaatatc agctttttta
     3601 tcaagagcaa ccttttgggc tttggcgtga ggactatcgt gctgctcaac ttgcccactt
     3661 aaccgcaatg atcaaccgtg acccaaaagg caaagcccct gaactctcgg aatttatgcc
     3721 gttttttaat cgggctgatg aagtagaagc aaatgaagat gatggcgttg cggattattt
     3781 ggcgaaaagg taaatctctt gtatttcatt gtaattctgt ctataattgg tgtaatacat
     3841 agaaatacag gagagataaa atggcgacta tcaatgatgc tttcagtttt agaacaaata
     3901 ccgaaataaa aaataccgca tttgatgtaa ttaaaaacta tggaatgaca ccctctcagg
     3961 tgtttaatat gtttttaacc gagattgcaa aaacgaaaac tattccgtta agtttgaatt
     4021 atcaacccaa tcttgaaaca aaattggcaa tgcaagaagc aaaatcaggt aaaaatgaag
     4081 tttatgcctc acttgaagca tttcataaag caatgttagc ggagtaaata atgctacaaa
     4141 tttcgccgac aaacgcatat aaaagagact ttaaaaagat tgcagccgaa ttagtcggta
     4201 gttcggaata tgtggaagta atgtattgcc taataaacca attaccattg gcggaaaaat
     4261 atagagatca cccactacaa ggtgaatggc aaggctttag agattgccat attaagcctg
     4321 atttagtgtt gatttacgct gttgaagata atctgctccg ccttgttcgt ttaggatcgc
     4381 acgctgaatt atttggataa tttctgtaaa aactaaccgc ttgcgatttt gtgctgtggg
     4441 cggtatagtt ggaatattaa caaaggaggg aaaaatgaaa aatgtacttc tatttatcgt
     4501 acactttttc ttaatcgcaa tgctctcaat tatcccaata ttaattattc ttggagcaat
     4561 cgaaagagat ccttactata ctgcgtggat tttccttagc gtatttttag gcattggagc
     4621 attgttatat tttgcaacca ttgtttctaa gttaattaac aagggaaaaa cacaaacatt
     4681 agaagaaaaa cgatatgcag aatattggaa taatgtaaaa gtcaatattc ccaaatagtc
     4741 taactgataa atgaccaaaa gctcgcttta tgcgggcttt tttattggag aaaaacaatg
     4801 agtggcttag gaaaattaac cgttacctta gagcttgaaa atgcaaaatt tcagtccgca
     4861 atgacgaaat ctgattatga ggcacaaaaa tttgccaaga actttatcca aaatatggat
     4921 agagctagaa atcacgccaa agaatttgcc gatcgctcaa cccaatattt aaaaaacatt
     4981 gagcaagcgg caaagaacct caataaaaat tctgaatttt catttttatc gactattggt
     5041 ggacattatc aatcaatgtc tagtggcata ttgagtgcgg caaaagccta tactgatatt
     5101 caaaataaaa tgaaattagt aagcggtagt tcagcagagg cagctaaacg attaactgat
     5161 gtgtttgata ttgcgactaa aacaagccaa agtacagagg ctgtttcagg tgtctatcaa
     5221 acatttgctc aaaatgccca atctctcgga ctggctcaaa aagatgtagc agaattaact
     5281 aaaacggtct ctcaagccgt tgctgcaagc ggcgcaagca gttctgcagc aagtaatgcc
     5341 ctaacccaat ttggtcaatc acttttaatg ggcaaaatga aagcccaaga atttaactcg
     5401 ctcattactc aaaccccaac cattattcaa gcaatggcaa aaggtttggg aatgacaatg
     5461 gcagaattta aagcagcggt ataccgggca aaatctttac ttggataaca ttcaagggca
     5521 aggttttgag agagtggata caccccaaat cggcgatgtg attttaatac aggtcggttc
     5581 tgatgtgccg aatcacgcag cgatttatat tggtgaacaa atggtaatcc accatagccc
     5641 gaaccgatta tctaagcgtg atttatatga cggttattgg ttacgccata cccacagtat
     5701 ttggcggcat aaactggcgg atagattgga ttttgacggc atatttaatg atataggaga
     5761 acaatatgaa atttagtaaa tatcaattat tagttaataa gatctgcttt atttgtggta
     5821 aaaaagattg tccacaaatg aaaaagagta aagactacaa agactttttg gatgctttag
     5881 aaaaagggga tgttgataaa gcagataaaa tatacaatac gaagttttca cagttttcta
     5941 aatcttttgg tcatgaaatg gaaaagaatt tagagaaaaa tcgtatacct cctatttatc
     6001 agggagttcc atttgattct tttgacttat atcagcatta ttcttatggt aatggagagg
     6061 cattaaatct ttcatccgtt ggattaagta ataaaattcg tcctctagtg aataaagata
     6121 aagcatttgg taaatccaat ggaagtatag aaagccgatt tatttcgcaa tataaaaatg
     6181 aaggacgcac atctttctcc aatgcttatg attttacaaa agaagcatcg ggtattgcag
     6241 atccgttatg ggcgttagga acagctacca ttagtggaga attgaaagat atacaaattt
     6301 ttcccggtgg tattgctagg ggaaatatcc attatagtat cagggacaga ttcactgacc
     6361 catttgatac attcaatttt actaaaggag aatggaaccc aaatggaaca ccttatatta
     6421 ttcaagatag ctggataaaa tcggtacgat tttcgataga c
//
LOCUS       DANLXQ010000140         6457 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.140, whole genome shotgun sequence.
ACCESSION   DANLXQ010000140 DANLXQ010000000
VERSION     DANLXQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.140"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            209..1435
                     /locus_tag="PXD94_002121"
     CDS             209..1435
                     /locus_tag="PXD94_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL6286943.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIDPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPVYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
     gene            1492..2868
                     /locus_tag="PXD94_002122"
     CDS             1492..2868
                     /locus_tag="PXD94_002122"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL6286944.1"
                     /translation="MISVFDMFKVGIGPSSSHTAGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDPVGGQVQ
                     VPCIERNAIASVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETSTGGL
                     AIKILPCD"
     gene            complement(2923..4512)
                     /gene="purH"
                     /locus_tag="PXD94_002123"
     CDS             complement(2923..4512)
                     /gene="purH"
                     /locus_tag="PXD94_002123"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL6286945.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVVGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            4684..4953
                     /locus_tag="PXD94_002124"
     CDS             4684..4953
                     /locus_tag="PXD94_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL6286946.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
     gene            complement(5040..6443)
                     /gene="dacB"
                     /locus_tag="PXD94_002125"
     CDS             complement(5040..6443)
                     /gene="dacB"
                     /locus_tag="PXD94_002125"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL6286947.1"
                     /translation="MLSTLLIPSITQAEINTEELLNTLPAGTSVSFIAKNLDTNQIIT
                     QYQSDIFMLPASTQKVFTALAAKLTLNDDFRFQTALLTNGKVENGVLKGNLIARFSGD
                     PELTSGQIYQLMSKLKQQGINKIEGDLILDPSVFASHDKASGWIWNDLTMCFNAPPAA
                     INVDHNCFYVTLNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPKEAPFCQLDVVVHDN
                     NRYQIKGCMARQSQPFGLSFSVQDPTNYGANMLKAQLKSLKIAFNGQVKEPLTAQNGT
                     LLAEHYSEPLPVLLKKMMKKSDNQIADALFRTVANKQHNRPASFQLGSYVIRQLLKTK
                     ANIDFKNSVVADGSGLSRHNQVSSRTMLETLEYIAQNEESLKLFETFPIAGVDGTISG
                     RGSISTEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFINGYSTGELESKTKR
                     APLNTFENKFYMALFNE"
BASE COUNT         1803 a         1258 c         1367 g         2029 t
ORIGIN      
        1 taaaagaaat ttccacataa aaataaaatt ctaaatttct ttgatttaag taaaatttaa
       61 ttttgtttga tggattttta ctcgccaaaa acactatttt ttctgctttt aatagtttga
      121 tcaaaaaata cgtaaaatct tgttgtgtta ctgtttccca tcgctaatat agcatagctt
      181 gttagataat tctttaaagg agattactat ggctatgcca tctcaacgtt tttctcttac
      241 ttgggtacta aatttatttg gtactgcggt aggagccggc gttttatttt tgccaattaa
      301 tgcaggtatg ggtgggttct acccacttat tattatgact ttgttagttg gaccaatgac
      361 atatttagct caccgtggct taacacgttt tgtgctttct tcaaaataca aagggagcga
      421 tattacggct gttgttcgtg aacattttgg tgaacaggcc ggtaaattga tcacgctact
      481 gtacttcttt gccatttttc cgattttact gatttatggt gtaggtatta ccaataccgt
      541 gagttctttt atggagaatc agcttcatat cgcgccacca tctagggctg tattatcgtt
      601 tatgctaata gctgctatga tcggagtaat gttattaagt gagcaagtca tgttaaaaat
      661 cacaacttgt cttgtgtatc cattagtgtt gattttattg ggcttatcta tttatttaat
      721 tccacagtgg aatacagctg ccttacagca aatgccaact acaggagact tccttactac
      781 actttggctg actattcctg ttttagtctt tgcttttaac cattcaccgg ctatttcttc
      841 ttttgcactt tctcaacaga aatattacca agatgacaag aaggctgaaa ttgaatcagc
      901 taaagtatta cgttctaccg cctttatctt ggtgttattt gtgatgttct ttgtatttag
      961 ctgtgtatta actttgacac ctgaagaatt agcacaagct aaagtgcaaa atatttcgat
     1021 tctttcttac cttgcgaata aatttgataa tccgattatt tcctattttg gaccgttggt
     1081 tgcttttttg gcgattggta gctctttctt tgggcattat ttaggtgcgc gcgaggggtt
     1141 agaaggattg gttaatcaaa tgcgtaaaga gccgattgat ccaagcaaat tccgcaaaat
     1201 taccgctatt acttttttga tcattttatg gattgttgcg actatcaatc caagcatttt
     1261 aggcttcatt gaaagccttg gtgggccgat tattgcgatg atcttgttta ttatgcctgt
     1321 ctatgccgta tataaagtac cggcacttgc acgttttaaa ggtgaatttg gccatttatt
     1381 tgtgcttgtt atgggttgta ttgctatttc agcaatagta tatgggttac tgtaatcttt
     1441 ctaaaatcaa tgtagaatac gcccttttat tttctatgtg aatttgtgtt tatgattagt
     1501 gtatttgata tgtttaaagt agggattggg ccttccagtt cccatactgc ggggccgatg
     1561 aaagcggcaa aggaatttgt cgatgaatta caaaatcgca aatttttaaa caaaattgac
     1621 cgcttgcggg tagatgtcta tggttcatta tcactcaccg ggcgaggaca taatacggat
     1681 attgccatca taatgggatt aatggggtat ttgcctcata atgtggatat tgaacgtatt
     1741 gagcagacga ttgaaaatct aaaacaaaat aagcaactta tcattgctga aagcagtgtt
     1801 gagcctaaag tgattgcgtt cgatttttat gaggatatgc catttcatcg agatttctta
     1861 cctcggcacg aaaatggtgt gaaatttagt gccttgagcg gtgaagagtt gctatttcaa
     1921 aacacctatt attctattgg tggtggcttt attgttgatg aagctaactt tgagcaagtt
     1981 aaaaattcag atctttctgt tccattccca tatcagaatg cagcagattt acttgcacat
     2041 tgtaagaata acgggttacc gctttcttct ttagtctgga aaaatgagct tgctttacgc
     2101 agtagagtag aaatttcgga ttatttggca atgatttgga atacaatgga agcctgtatt
     2161 gagcgtggtt tgaatacgga agggttacta cccgggcctc tgaaagtggt acgccgtgcc
     2221 agtgggctac gcagaatgtt agaggcaaac agtaaattca ataccgaccc aatgcaaatt
     2281 atcgattggg tgaatatgtt tgcccttgcc gtgaatgaag aaaatgcggc aggtgggcga
     2341 gtagtcaccg cacctaccaa tggagcctgt ggtattgttc cggctgtgtt ggcgtactac
     2401 caaaaattta ttgcacagct taatcaagat actattgagc gttatttatt gactaccagt
     2461 gtgattggtt cgttatacaa aatgaatgcc tctatttccg gtgctgaagt aggttgccaa
     2521 ggggaagttg gtgttgcttg ttcaatggcg gcagcaggtt taacggaaat tatgggtggc
     2581 agtgctgaac aggtgtgtat ggctgcagaa attgcaatgg agcacaattt aggcctaact
     2641 tgtgaccctg ttggcggaca agtgcaagtg ccttgtattg aaagaaatgc aatcgcttca
     2701 gtcaaagcga ttaatgccag cagaatggca ctacgccgta cttcttcgcc tcaggtaagt
     2761 ttagataaag tcattgaaac aatgtacgaa accggcaaag atatgaatgc aaaataccgt
     2821 gaaacctcaa ccggtggctt agcaatcaaa attttacctt gtgattaatt aatgaaaaag
     2881 gctaccttat caaaataagg tagcctttta gtttgtagaa cattaatgac ggaaatgcct
     2941 cattccggtt agtaccactg ccataccgtg ttcgttggcg gcatcaatca cttcttgatc
     3001 acgcattgaa ccacctgggt gaatcacaca agtaatccct acttttgcag cagcgtcaat
     3061 accatcacgg aacgggaaga acgcatcgga agccattaca caacccacaa cttgtaagcc
     3121 ctcgtcttcc gctttaatac ctgcaatttt tgcggaataa acacggctca tctgccctgc
     3181 accgataccg atagtttgcc cgtctttggc atacacaatc gcattcgatt tcacatattt
     3241 cgccactttc cagcagaaca ataggtcttt tagctcagct tcagttggtt tacgctctga
     3301 aaccaccttt aaatcgtcaa tagatacaga gccttggtcg gcatcttgca ctaataatcc
     3361 gccgtttacc cgtttgaaat ctaagcgttt tacagcttct gagaactcgc cacattcaag
     3421 cacacgcacg tttttcttag tggctaacac ttctttggca gaagcagaaa cggttggtgc
     3481 aataatcact tcaacaaatt gacggtctgc gatggtttga gcagtttcgc cgtctaattc
     3541 acggttaaag gcaataatgc caccgaaagc agaagttgga tcggtttggt aagcacggtt
     3601 gtaggcatct aaaatatttt tacccaaagc aacaccacaa gggttagcgt gttttacaat
     3661 cacacaagca ggctcgctaa attctttcac gcattcaaga gccgcatcgg tatctgcaat
     3721 attattatac gaaagggctt taccttgtaa ctgtttggcg gtggaaacag aggcttcttt
     3781 taactcattt tcgacataga atgcagcgtt ttgatgcgaa ttttcgccat aacgcatcgt
     3841 ttgtttacgc acaaaattga ggtttaaggt gcgagggaat tgtccacttg gttgggttaa
     3901 atcttcttcc tcagcccctt gataaggcgg cactttttgc ccgaaatagt tggcaatcat
     3961 tgaatcgtat tgtgcggtat gctcaaacgc tttaatagca agattaaaac gggtttctaa
     4021 agttaaactg ttttgatgtt gatccatttc ggcaagaata gcatcaaaat cattgttatt
     4081 gaccacaatc gccacatctt tatgattttt cgctgcagaa cgcaccattg tcggaccacc
     4141 gatatcaata ttttccaccg catcttcaag gctacaattt ggattggcaa cggtttgagc
     4201 aaaagggtag aggtttacta ccaccatatc aattcgttca atgccgtgtt ggttcatcac
     4261 ttcatcatca gtaccacggc gacctagaat tccgccgtgc actttcgggt gcagggtttt
     4321 tacacgcccg tccatcattt caggaaagcc ggtgtaatct gatacttctg ttactagcaa
     4381 accggcttca gccagtaatt ttgcggttcc gcccgtagaa agtaatttta ccccacgagc
     4441 cgatagccca cgagcaaatt cgacaatgcc ttttttatca gacacactta ataacgcctg
     4501 ttgaatcgcc attcttaaat tccttaaaat gagtgttaac gtgaaaataa acggcaaaga
     4561 ttataacgca aacgtttgct tttttcatct acaagtggtc gattttgcaa atttttctgc
     4621 aaaatcgacc gcttgcactc gtgattaaag cgggtagaat agaggaaaaa aatcggagga
     4681 actatgatta accacaattt agcagatttc gtatttgagt attttaccga aaacaaccta
     4741 aaagcaggca cactacgtta tcgctacata aaagagaacg tgattgatgc ttacagtacc
     4801 cgagtagatg atgcctttca aggtaaaggt attgcaggag agctttacaa tgctttaatt
     4861 gcgtttgcgc aagaaaaagg gctgaaaatc aagccaagtt gtagttatat tgaggtaaaa
     4921 atgcaacgaa gtcatcgaga tttaattgct tagagaggag caagcggtcg taaaatttca
     4981 aaaatttacg accgctgtct tattcaattc tcaaaaatcc atcacacaaa atataagccc
     5041 tattcattaa acaacgccat ataaaactta ttttcaaatg tattaagcgg agcccgtttg
     5101 gttttgcttt cgagctcgcc ggtggaatag ccgttaataa attgcacaaa agcgatgcgt
     5161 tcgccacggg cgtttttcat aaagccggca aggttgtaaa cccctttcag tgagccggtt
     5221 ttggcaatca gatttttggc aagcggttct gttgaaatag agcctctgcc ggaaatagta
     5281 ccgtccacgc ctgcaatcgg gaaggtttca aacagtttta agctttcttc gttttgagcg
     5341 atatattcaa gggtctccag cattgttcgg ctgctgactt gattatgacg ggaaagccct
     5401 gagccatcag ccaccacgct gtttttaaaa tcaatattgg ctttggtttt taataactgg
     5461 cgaatcacat aactacctaa ttggaacgat gcggggcggt tgtgttgctt gttggcaacg
     5521 gttctaaaca gggcatcggc aatttggtta tccgattttt tcatcatttt tttcaataat
     5581 accggcaacg gctcggaata atgctcggca agtagcgtgc cgttttgggc agtaagtggc
     5641 tcttttactt gcccgttaaa ggcaattttc aggcttttta actgggcttt aagcatattt
     5701 gccccgtaat tagttgggtc ttgcaccgaa aaacttaagc cgaacggttg agactggcga
     5761 gccatacagc ctttgatttg gtagcggttg ttatcgtgaa ccaccacatc aagctggcaa
     5821 aacggagctt ctttcggctc gacaatataa gcagagctaa acacctgcac cggataagcg
     5881 gacggcacat tcactttagc aaattcgcca atgggttggt cggcatttaa ggtgacataa
     5941 aaacagttgt gatccacatt aattgccgcc ggtggggcgt taaagcacat cgtcaagtcg
     6001 ttccaaatcc aaccggaggc tttgtcgtgg ctggcgaaaa ccgacgggtc tagaattaaa
     6061 tccccctcaa ttttattgat accttgttgc tttaatttgc tcattaattg ataaatttga
     6121 ccgcttgtaa gctcagggtc gccactaaaa cgggcaatta aattgccttt taacactccg
     6181 ttttctactt ttccgttggt taaaagggca gtttgaaagc ggaaatcatc gtttaaagtt
     6241 aattttgctg ccagtgccgt aaagactttt tgggtactgg caggtagcat aaaaatatca
     6301 ctttggtatt gggtgatgat ttggttagtg tctaaatttt tagcaataaa actaacagaa
     6361 gtaccggcag gcaaggtatt taataattct tcggtattaa tttcagcctg tgtaatgctt
     6421 ggaatcagaa gtgtagataa gataaattta tttaaaa
//
LOCUS       DANLXQ010000141         6439 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.141, whole genome shotgun sequence.
ACCESSION   DANLXQ010000141 DANLXQ010000000
VERSION     DANLXQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.141"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            101..2470
                     /gene="malP"
                     /locus_tag="PXD94_002126"
     CDS             101..2470
                     /gene="malP"
                     /locus_tag="PXD94_002126"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL6286948.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
     gene            2552..4630
                     /gene="malQ"
                     /locus_tag="PXD94_002127"
     CDS             2552..4630
                     /gene="malQ"
                     /locus_tag="PXD94_002127"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL6286949.1"
                     /translation="MKITAKQYYRAGIMPYFFDERGVKKTASEKIKHSLFKAFNGNTQ
                     ARETPIPTVNIFYQHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLP
                     QDLPLGYHQLQLKTVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNW
                     GIGDFGDLKDFIQQIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDIN
                     QLAEFQQSDEAQTWFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMT
                     ACAAPAFIEFVQKGGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQ
                     FRETHKTDIEFYLWLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDK
                     QLYCLNASVGAPPDVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLD
                     HIMSLLRLWWIPKGDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIV
                     SKLKNAGILSYKIFYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELG
                     EKFGVYPNSNILAILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQ
                     TYVANVSSGLFGFQPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDKDIQALL
                     KEVNLIRKNQ"
     gene            complement(4695..6356)
                     /locus_tag="PXD94_002128"
     CDS             complement(4695..6356)
                     /locus_tag="PXD94_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL6286950.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRSKDQLSTLNVRLTNPNIHGL
                     TLKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
BASE COUNT         2063 a         1392 c         1207 g         1777 t
ORIGIN      
        1 acttccttat aatcatacca tacaagatga ttattgggat gatttgattt tatttggatt
       61 ttacataata gcgacattca tctttaaccg ggggtttcct atgacattta attctattgt
      121 agaaaaatac tgtcgctact ttgatgtgac agatccaaaa cactttacct tacagcagtg
      181 gtatcaagtt gttgctgaag gttcccttga gctaatttat agccagcctg caaccaaacc
      241 gacagaatca cgccacgtga actacctctc aatggagttc ttaatcggtc ggttaaccgg
      301 caataattta atgaatttag gttattacga acaaattcgt gactatctta aacaatatca
      361 agttgaatta gtagatgttt tagaacaaga gcgtgatccg gcattgggta atggcggatt
      421 aggtcgtctt gctgcctgct ttttagattc gatggcaact gtcggacaaa atgcaaccgg
      481 ctatgggctt cattatcaat atggtctgtt taaacaaagc ttcaaagagg gaatgcagaa
      541 agaagcagcg gatacttggg ctcgtgacag ctacccgtgg catcgtttta atccgtctaa
      601 aacccgccat atcgggtttg gtggaaaaat caaacatatc caagccgatc aatatgaatg
      661 gcagcctaaa ttgaccattc aaggcaaagc ctttgacttg ccgattgtag gttataaaaa
      721 caacatcatt caaccattac gtttatggca agcagatagt gaccaatctt ttgattttga
      781 tgcctttaat gagggtaaat ttttaagtgc cgataaaacg attgtcaatg cagccgcact
      841 aactcaagtg ctgtatccaa acgacaacca tcaagccgga caaaaattac gcttaatgca
      901 gcaatacttc cattgtgcct gctcggttgc cgatatttta gatcgtcaca ttagcgaagg
      961 ttattcgtta gaagatttag caaaacgcca agttattcaa ctcaatgata cccatccaac
     1021 gctcgctata cctgaattga tgcgtgtact gttagatgaa catcaattca cttgggagca
     1081 ggcttgggat atttgcagca atacctttgc ttacactaac cataccttat tacctgaagc
     1141 acttgaacaa tgggatcaac gcctatttaa gcagctatta cctcgccatt atcaaattgt
     1201 ggaaaaaatc aatgatattt tccacgacaa agtacaggca gaatttggtg atgatgcaaa
     1261 aatttgggaa aaattagccg tgctatttga ttaccgtgtg cgtatggcaa acctctgcgt
     1321 tgtcacctgc ttccgtgtaa acggcgttgc ggaaattcac tccgatctct tagtgaccga
     1381 tctattcccg gaataccaca aattattccc aacaaaattc tgcaatgtta ctaacggcat
     1441 cacacctcgc cgctggattc gacaagctaa tccacaatta agtgatttat tggatcgtac
     1501 cttaaaacaa gattggacac acaatctgga attattaaaa ggcgttgaac aatatgcgga
     1561 tgacaccggt ttccgtgaag aatatcaacg cattaaacgc aataacaaag tggttttagc
     1621 caatgaaatc cataaaacat tagggttaaa tgtcaatcct aatgcgattt ttgatgtgca
     1681 aattaagcgt ttccacgaat ataaacgcca acatttgaat ttgttaaaca ttattgcgac
     1741 ctatcaatcg ctaaaagcta acccgaacca agactacaca ccacgtctat tcctattctc
     1801 gggcaaagct gcaccgggtt attatttagc aaaaaatatt attcacgcta ttaataatgt
     1861 ggcagatatt attaataacg acaagcaggt aaatgatcga ctacaagtgg cattcttacc
     1921 ggattatcga gtcagtcttg cggaaaaaat tattccagca gctgatgtat cagaacagat
     1981 ttcaatggca ggtaaagaag cctccggtac cggtaatatg aaattggcac tcaacggcgc
     2041 attaacctta ggtacgctag acggtgccaa tgttgaaatt gctgatatgg tcggtgagga
     2101 gaatgtcttt atcttcggtc acaccgtaga aagcgtgagg gaattattag caaaaggcta
     2161 ccaaccgaaa gcctactata accaagatcc gattctgaaa aatgcggttg atttcttagc
     2221 cacaggaaaa gcctctaacg gagataaaga ggcattcaag ctgatgttag atagcctgtt
     2281 ggaacgagat cctttcttag tctttgccga ctttgatagc tatcgccaag cacaagagaa
     2341 aattggtgta gcctatttag atcaagaaac ttggctgcgt agtgcaattt taaatactgc
     2401 tcgtttgggt gcatttagct cagaccgctc aattaaagat tatcaacaac gtatttggtt
     2461 gaatagataa taaattcggg cgacttctac aataaaggaa gtcgcctatt ttctgctgta
     2521 tttcatatgt atttatatat ggagagaaat gatgaaaata actgccaaac aatattatcg
     2581 ggctggaatt atgccctatt ttttcgatga gcgtggtgta aaaaaaacag catctgaaaa
     2641 aataaaacat tcgttattca aagcctttaa cggcaatact caagcaagag aaacacctat
     2701 ccctacggtc aacatttttt accagcacca accctgtttt ttaccttttc atttagcgga
     2761 taaaaaacat ccgttaaccg gtacttggca gctacaacta gaaaattcaa accaaattat
     2821 ttccggcaaa attaaagcaa ggggaattac attacctcaa gatttgccct taggttatca
     2881 ccaattacag ctaaaaacgg tagataaaac ctttaattgc accattatta tcgctccgga
     2941 acgctgctac caaccacaag ccgtcattga acaaaaaaga ttgtggggaa ccttcctaca
     3001 actatacacc ttaaaatcag cccacaactg ggggattggc gattttggcg atttaaaaga
     3061 cttcattcaa cagattgccc cttaccaagc agattttatc ggcttgaacc cgattcactc
     3121 actttttccg gcaaacccga attctgccag cccatacagt ccatcctctc gccgatggct
     3181 gaatattgct tacattgata tcaatcagct agcggaattt caacaaagtg atgaagcaca
     3241 aacttggttt cattctgctg aaatccaaca acaattatca gaattacgca ctcaggattg
     3301 gttgaattac gagcaaatca ttccgttgaa attgaaaggg ctacgctttg ctttcgcaaa
     3361 atttcagcaa aatatgaccg cttgcgctgc ccctgctttt atcgaatttg tacaaaaagg
     3421 tggtgaaagc ttacaagttc aagccacatt tgatgccctt catcagcatc tatcggcaca
     3481 atttgccgac caatggggct gggatttctg ggagaaagcc taccaagact accactccgc
     3541 agccgtgcag caatttagag aaacgcataa aaccgacata gaattttacc tttggttaca
     3601 attcgttgcc gatcaacagc tagcagaatg tgatgctatc tgccgtcagc aaaatatgcg
     3661 tattggtatg tatcgcgatc tcgccgttgg cgtcagcccg aatggtgcag aaacgtggaa
     3721 tgacaaacaa ctttattgct taaatgcctc tgtcggcgct ccgcctgatg tattaggtcc
     3781 gcaaggacaa aactggggct taaccccaat gaatccgcat gtattaaagc aacaagccta
     3841 ccgtccgttt attgagttag tcagagctaa tatgagtcat tgcggtgcat tacgccttga
     3901 ccatattatg tctttactcc gtttatggtg gatccccaaa ggcgatagtg cggttaatgg
     3961 tgcttatatt cgttatccag ttgatgattt aatcgcaatt ttggcacttg aaagccaacg
     4021 ccaccaatgc ctaattatcg gtgaagattt aggtactgtg ccaaaagaaa ttgtgagcaa
     4081 gctcaaaaat gcggggattt tatcttacaa aattttctat tttgaatttg atgaacaagg
     4141 gaagagtcga gacttacagc attaccctta ccaagcaatg accacattaa gcacccacga
     4201 tttaccgact atcaatggtt attggcgtgg ctatgatttt gaattaggcg aaaaattcgg
     4261 cgtctatcct aattcgaata ttttggcaat ccttaagcaa gatcgagtca atgccaaagc
     4321 caaaatctta caacgcttac aagaacataa cgtagagatc gcacctgaaa ttagcgagca
     4381 acttaattct ccggtgaata ccgcatttat tcactgttta caaacctatg ttgcaaatgt
     4441 aagcagcgga ttattcggct tccaacccga agactggttg ggtatgactg agccggttaa
     4501 cattccggga accagtacac aatatgcaaa ctggcgacgt cggcttagtg ttaacctcga
     4561 ggagatcttc acagataaag atattcaagc cttattaaaa gaagttaatt taatccgtaa
     4621 aaatcaataa aagttagata agaaaaaagg cgactttccg tcgccttttg ggcttatatg
     4681 cttgatgtct atctttatcc acctgaaacc cacaacataa tcgctagcac ttgaggtagc
     4741 ataatacgca aaaacattac tagtgggtaa acagttgcat aggataacgc tgcagcgcca
     4801 tttccctctt taatttcatt ggcaaaagca agcgctggag gatcagtcat tgagccggca
     4861 agtaatccac aaatcgttaa atagtttact ttgttataca ttctggcaat caatcctgct
     4921 atcatcaatg gaacaaaagt aataatcgcc ccatagcata tccactcaac gccctcactg
     4981 cttaatagcg tttcgagaaa tctaccgcca gattttaaac caactaccgc taaaaacagt
     5041 acaataccga tttcacgcaa tgcaagattg gcacttggtg gcataaacca gtagagttta
     5101 ctgaaactac caattcttgc aagaataaga gcaaccacca gtggtccacc agccaagccc
     5161 agctttaacg caacagggaa acccggtaaa taaatcggta cagaaccaag taatactccc
     5221 aaaccgatcc cgataaatat tggcaacatt tggacttgtt gcaactttga gtgcgcattg
     5281 ccgataatcg tcattaccat ttcaatatca gcttgccgcc ctactaaatt gagaacatca
     5341 ccaaattgta acgtcatatc acctgttggt actaattcta caccggctcg atttaaacga
     5401 gaaatgacaa catcgtattt gcctttaagc attagctgac gaatttgttt cccgaagact
     5461 ttttcattcg tcactacagc acgttcagaa cggtaaatcg tgcctttggt tgagacagat
     5521 acatctactg cctcacctaa aataatctgc atttttcgta atgaggattc ttcacctact
     5581 aagtgtaaaa tatctcctat ctgtagaatc gtatctacac gtggaataga aagctcattc
     5641 ccccgtttta agcgtgaata agctacttcg tgcaaattaa aatcaggaat ttgtttcaac
     5701 gttaaaccgt gaatatttgg attagtcaga cggacattta aggtactaag ttgatcttta
     5761 cttctatttt gctgtttgtt aaaagcctct gcttccgcct caatattgat tcgaaataaa
     5821 atccgaatca gccacattga aagtagaatt ccgataatgc cgaacgggta agcaatcgca
     5881 taactcattc ccataatttc agttacactc tcagcgctta actcagccaa tacctgttga
     5941 cctgctccta aagaaggggt attggttaca gccccggaga aaatgcctaa aatcacaggc
     6001 aaaggaacat tgaagaattt atgaataaga ataactaaaa taccgctaat cagtacaatc
     6061 agtaccgcaa aaccattgag ttttaagcca gaatgtttta gtgaggcaaa gaatccaggt
     6121 cctacttgaa ttccaattga ataaacaaat aaaatcagcc cgaactcttg aataaaatga
     6181 agcgtatggg catcgagctt cacatcgaat tgcgccataa aatgggagac aaacagccca
     6241 ccaaataaca ctccccctat acctagcccc acgcctctga ttttaatatg gccaatccat
     6301 aagcctagaa cggccactaa agataataaa ctgacagtaa gtgcaatttc gctcattaca
     6361 gcctctcttt aatatgaaat acctcaaaaa tggtatccaa ttctaccaaa tctcaaaaac
     6421 gaaaaatgag atctatgtc
//
LOCUS       DANLXQ010000142         6251 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.142, whole genome shotgun sequence.
ACCESSION   DANLXQ010000142 DANLXQ010000000
VERSION     DANLXQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.142"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(29..427)
                     /gene="mutT"
                     /locus_tag="PXD94_002129"
     CDS             complement(29..427)
                     /gene="mutT"
                     /locus_tag="PXD94_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL6286951.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
     gene            complement(514..3240)
                     /gene="secA"
                     /locus_tag="PXD94_002130"
     CDS             complement(514..3240)
                     /gene="secA"
                     /locus_tag="PXD94_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL6286952.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGVSLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIERKQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            complement(3299..3619)
                     /locus_tag="PXD94_002131"
     CDS             complement(3299..3619)
                     /locus_tag="PXD94_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL6286953.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            3740..4042
                     /locus_tag="PXD94_002132"
     CDS             3740..4042
                     /locus_tag="PXD94_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL6286954.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQGLILQQNMLLKQIQTDLPEITQLEFRVNPSVT"
     gene            4067..4441
                     /locus_tag="PXD94_002133"
     CDS             4067..4441
                     /locus_tag="PXD94_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL6286955.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(4617..4706)
                     /locus_tag="PXD94_002134"
     tRNA            complement(4617..4706)
                     /locus_tag="PXD94_002134"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4670..4672),aa:Ser,seq:gga)
     gene            complement(4798..5109)
                     /locus_tag="PXD94_002135"
     CDS             complement(4798..5109)
                     /locus_tag="PXD94_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL6286956.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSLNCLGVG
                     Q"
     gene            5201..5788
                     /locus_tag="PXD94_002136"
     CDS             5201..5788
                     /locus_tag="PXD94_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286957.1"
                     /translation="MKRSKKTLVLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNN
                     QTAAVNVMTQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQG
                     AANANVVVNIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFG
                     ASNDEIKKVLDQAYSDVVKAIYNDNEIANAIHQFK"
     gene            5960..6133
                     /locus_tag="PXD94_002137"
     CDS             5960..6133
                     /locus_tag="PXD94_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286958.1"
                     /translation="MNNPYHNVSQEYLDYLAKKVKQGKEDIKNGRIYSPEEVRKRVIA
                     AAFRGAENAKKVA"
BASE COUNT         1930 a         1322 c         1145 g         1854 t
ORIGIN      
        1 ttactttaga tacagttctc aatacaaatt atgcgtgttc ttttaagcgt ttaattaatt
       61 gggcatttgc cggtgggaaa gctccctcat ctaaatccga ttgtgcgatc caaaaacctt
      121 cttgcccttc tcttccgtaa ggttcattta cccactcttc caccaaataa aagaaaaatt
      181 caatgatttt tgttggatat tcaaagcaaa aattttcata cggaaaagcg cttaacactt
      241 gaataccaat ttcttcttct aattctcgtt ttagtgcttc ttcaggggtt tcattgggat
      301 cgactttccc cccgggaaat tcaagagact gagcaaaatc ttgcccttct aaacgttggg
      361 ttaagtaaat ttgcccaaat tcgttacgaa taatccctgc tgagacttga ataatcggtt
      421 ttgacatagt ttccttcact ctttctataa attcaagcgg tcatattaag cagaaaattt
      481 gcaaattcct gactgaatat gaccgcttgt taattacgaa tatctcgctt tgttaccatg
      541 gcaatgctta tattttttgc ccgaaccaca agggcaaggg tcatttcgac caatatttaa
      601 gtttgccaac tcttcttcac ttaagctctc tgcgctattt tcttctctac taccagcagc
      661 agaatcggta gcttgtgtaa ctggcgtact tgcagcgtcc atttcagctt gctggcgttg
      721 cgcttcttcc acctcttctt tgctacgcac ttgaatacgg cttaaaatgc taattacatt
      781 caattttaag gtatcaagca tattggtaaa catcgcaaaa gattcctttt tatactcttg
      841 cttcggatct ttttgtgcat aaccacgcaa gtgaatacct ttgcgtaaat aatccattga
      901 ggctagatgc tctttccaaa gctcatctaa gttttgtagc attacacctt tttcaaaatt
      961 acgcattacc tctgcgccta ctatttcttc cttcgcattg tactctgcaa tcgcaatatt
     1021 taagatacgt tcacgcaaag tctcttcgtg caagtctttt tcagtttcaa gccagtgaga
     1081 aatcggtaaa tccaacccaa attcacggcg taaacgctct tctaagcccg gcacattcca
     1141 catttcctcg atagattgag gcggaatata ttggttaatc gtgctttcaa atacatcttg
     1201 gcgaatgctt tcgatcattg aggcaatatt atctgtttct aatagatgat tgcgttgttc
     1261 gtaaattact ttacgctgtt cattggcaac atcatcatat tgtaataaat gcttacgccc
     1321 atcaaagtta tgcgcttcta ctttcgcctg tgcagaggca atcactttgg ttaacagttt
     1381 agactccatc gcttcacctt cttcggtaaa ggctttacgc atcatattta atttaccttc
     1441 attgaggtaa attctcatta gggcatcatc taaagagaga tagaaacgtg atgaccccgg
     1501 gtcaccttga cgaccagaac gacctcgcaa ctggttgtcg atacggcggg attcgtggcg
     1561 ttctgtacca ataatatgta aaccaccggc ttgcattacg atgtcataac gctcttgcca
     1621 cgcagcttta atcgcatcaa tttgttcttg tgtcgggttc tcaagcttgg ctacttccat
     1681 tttccagtta ccgccaagca caatatccgt accacgacca gccatattgg ttgcgatagt
     1741 aaccgccccc ggagctcccg cttccgctac aatttcagcc tcttgagcgt ggaatttcgc
     1801 atttaatacg ttatgagcaa taccggcttt ggttaattct gccgataata actctgattt
     1861 ttccaccgat gctgtgccaa ccaaaacagg ctgtttacgc tcgatacagt ctctaatttc
     1921 agtaataatc gccgcaaatt tctccgcttc gcttttaaac attaagtctg tacggtcatc
     1981 acggatcacc ggtctgttgg ttggaatcac caccgtattt aagccataaa tttgctggaa
     2041 ttcaaaggct tcggtgtctg ccgtaccagt cataccggct aatttttcgt acaaacggaa
     2101 atagttttgg taagtaattg aggcaacagt ttggttttcg ccctgaatag ccacacgctc
     2161 tttagcttca atcgcttggt gtaagccgtc agaccaacga cgtccagcca ttgtacgacc
     2221 ggtgtgctca tcaataatca caatttcacc gtcttttaca atgtaatcta cgttgcgttc
     2281 aaataattta tgggcacgca atgctgcact cacatggtgt aacagcgaaa tacgagcagg
     2341 gtgatataaa ctttcgccct cttccattaa ccccatttga gttaagaaat cctctacttt
     2401 caccatacca cgctcggtta aatgagcctg tttgtttttc agatctaagg taaaatcgcc
     2461 ctcaccggtg tactcttcac tgtcttcttt atcttgagca atcaaatgcg gaatgatttg
     2521 atctaccgct tgataaattt gggtcgcatc ttccgcaggc cctgaaataa ttaacggcgt
     2581 acgagcttca tcaattaaaa tagagtctac ttcatcaact aatgcgtagt gtaactcacg
     2641 ttggaaacgt tcgtttttat cgtgagctaa gttatcacgc aagtaatcaa aacctaattc
     2701 gctgttagtc gaataggtaa tatctgcttg gtatgcagca cgttttgcat cggaaggtaa
     2761 ccccggaata ttcactccaa cggttaagcc taaaaactca aataacggac ggttggtttc
     2821 cgcatcacgg cgggcgaggt aatcattcac cgtaacaaca tgaacacctt taccggtcaa
     2881 agcattcaaa taacaagata aagttgcagt tagggtttta ccttccccag tacgcatttc
     2941 tgcaatatta cgccccgtta gcaccatacc gccgatcagc tgtacatcaa atggtcgcat
     3001 acctaatact cgtttgcttg cttctctcac tgtcgcaaaa gcttcaggta ataggctatc
     3061 tagcgataca ccatctgcaa ggcgttgctt aaactccgct gttttatcct ttaacgcctc
     3121 atcagataat tgctcgaagg ttggttcaag cttattaatt tgtgccactc gctttcttaa
     3181 acgttttaaa gtgcgatcgt tgcttgagcc aaaaatagaa gtcagtaatt ttgaaatcat
     3241 tgtgttattc ctaaatataa aaatgggtta atcaattaat ggtaaaatta aattatattt
     3301 aggtttgtgg cccggctcga atggggtaag cattttgtct atccaagcgg taggagtttg
     3361 caaaaaattc gcaaaaatgc accgcttgtg gtttaatttc cacaatttta tggattaact
     3421 ctgcaataaa aagcgattgt tgctcaatat cattagcgga ttgagcatat tgaagaaaag
     3481 attgattaag aacggtttcc tgttcacttt cattggcgta cgcaacctga atttcaggta
     3541 aagcaagtat cgcaaccatt cccaaaaaga gttgcgacca aaaaggcgtt cgatgaaagt
     3601 gttttaaaaa taagctcatt aaattaagat aaatgaaaaa attgggctaa ttatacgcta
     3661 aagtaaagcc gaatgagtaa attattgggt caagtatcgt aaaatcaagc ctaaaataag
     3721 acgaaatgta aagaaacaga tgaaaaattc agcacctaaa aagattaatg acattttaca
     3781 gaactcaagc ttaagccgta ttgtcgaaaa agcaaacttc attaacgatt taaaccaaaa
     3841 aattcagaaa agatggccag aatcttatcg tgggctttac cgagtagtga atttagttga
     3901 taatcagctt attgtgagcg ttaagaatgc aaccgtacgg caaggtttaa tcttgcaaca
     3961 aaatatgtta ctaaaacaga tacaaaccga tttacctgaa ataacccaac ttgaatttag
     4021 agtaaatccg agtgttacat aatttaattt tgaagaggaa aatagtatga aaaaaatcgc
     4081 actcattggt tttaccaccg caatgctagg ggcttgctca ttgctaccac aaaaaagcgt
     4141 tagcggtact tatgcaggtg aattaccgtg tgctgactgt gagaaaatcc aagcccagct
     4201 aaccttaaat gcagataaaa cctaccaata tgatacggta tattttaaag ataaaaaaga
     4261 atacacttac cgagacaaag gcacatacag ttgggaaccc aataagccaa atgtgattcg
     4321 tctggaaaaa gcatcaggca atcttgcctt ccaagtgtct gaacaacacg ttgaactttg
     4381 cgacccaaat ggcaacacgg taaaaaacag caataactat aaactaggta aagtacgtta
     4441 aaagcagata aaaaggattc ttaggaatcc tttttctttg taatctatcg ccgatcgcat
     4501 aaaaaaaccc ccaagcaatg tttgctcggg gataacctaa aaatgaaaaa gtattatcta
     4561 ctttaactta accaaggaaa tatctatgaa aacagtaagg aatctttcag tatgaatggc
     4621 ggtgagaggg ggattcgaac ccccgataca gtttcccata tacacgcttt ccaggcgtgc
     4681 tccttcaacc actcggacat ctcaccgaat caacaaaaag atggcgtaaa tatagcaatt
     4741 ttacacggct ctttcaagta ttttcttcac aaaataaaat aaatcatttt tatccactta
     4801 ttgcccaaca cctaagcaat ttagcgattt aggtacaagt ccgccagtcg cttcccattc
     4861 accttgggta tataggtgca aagcaagtgc gtgaacgcca ttagctaatt cctcagctaa
     4921 gcaagcatag acagcacgat gacgtgcaac tgttctctgc ccttcaaatt tatctgacac
     4981 caaagttaat ttaaaatgcg attctgctcc tctacctgag ctgtgcatat agctttcgtt
     5041 ttcgacatta aagaaaatcg gctgaaattc tgtcttaatt ttttgaataa ttgtctgttc
     5101 taccgacatt ttttgctctc gttcattgaa aatcaaaacg aaataatact atactaagca
     5161 gaaattttct cgaaatttat tcaatataga aggatttact atgaaacgct ctaaaaaaac
     5221 gttagtgtta gccactactt ttgttgccgc attactagcc ggctgccaaa gccaatccaa
     5281 cacattaaca ttctctaccc caacgccaac tgctgtattt aacacaaaca atcaaacggc
     5341 tgcggtgaat gtaatgacac aagatttacg cacctcgcgt gaaatcgcaa gctacactaa
     5401 aaacggagag gtcattcgtt taaccgcagt gcctgaagtt gctgcaatgt tccaacaagc
     5461 cgtccaacaa gacctgaatg caaaaggttt ccaaattgta caaggtgcag cgaatgccaa
     5521 tgtggtagtg aatattcgta aattctttgc cacagtagaa caaggaaact tacgctacaa
     5581 agccgatgca caagtgagtg taaaagtggc agtgcaaggt tctaaaggaa actttagcaa
     5641 aaacttcaac gctacccgtg gttacgaagg tgcatttggt gcaagcaatg atgaaatcaa
     5701 gaaagtgtta gaccaagcct attctgatgt ggtgaaagct atttacaacg ataatgaaat
     5761 cgccaatgca attcaccagt tcaaataatt aattttttct ggcaagcggt catctttctt
     5821 cgattttttg caaaaaacgt ggaaaaatga ccgcttgttg ttttctcgtt ttgtgatgtg
     5881 gctcgaaaaa taagataaat taacttgatt aaacgatcag tagcccccac aatagagact
     5941 catcgcaggg aggaaaaata tgaataatcc ttatcataat gttagccaag aatatcttga
     6001 ttatcttgct aaaaaagtaa agcaaggaaa ggaagatatt aaaaacggtc ggatttattc
     6061 acccgaagag gtcagaaaaa gagttattgc tgccgctttt agaggtgctg aaaatgccaa
     6121 aaaagttgca taatatttat tatactgaag aggccttaat agatttagag aatattgcca
     6181 tctcagttag tgaatttacc ggttatgcaa gtagtggaat taggatacta gaagaattgg
     6241 aaaatagtat t
//
LOCUS       DANLXQ010000143         6150 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.143, whole genome shotgun sequence.
ACCESSION   DANLXQ010000143 DANLXQ010000000
VERSION     DANLXQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.143"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            24..1940
                     /gene="iolC"
                     /locus_tag="PXD94_002138"
     CDS             24..1940
                     /gene="iolC"
                     /locus_tag="PXD94_002138"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL6286959.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            2229..3569
                     /locus_tag="PXD94_002139"
     CDS             2229..3569
                     /locus_tag="PXD94_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL6286960.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTELKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            3572..4912
                     /locus_tag="PXD94_002140"
     CDS             3572..4912
                     /locus_tag="PXD94_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL6286961.1"
                     /translation="MSVSQRFIDFMNAHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELLEKARMAAQ"
     gene            5050..5874
                     /locus_tag="PXD94_002141"
     CDS             5050..5874
                     /locus_tag="PXD94_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL6286962.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQVSEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(5918..>6150)
                     /locus_tag="PXD94_002142"
     CDS             complement(5918..>6150)
                     /locus_tag="PXD94_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL6286963.1"
                     /translation="SIILEIINALGNKENIKQVEACLTRLRVVLHNNGLLNKSALKKL
                     GAVDVIKVGDTQQIIFGAKAGQYRDEINALIG"
BASE COUNT         1737 a         1312 c         1384 g         1717 t
ORIGIN      
        1 tttacttgtt gtaaggagcg gttatggcaa cagttaatca tcaagatcgt ccattggatc
       61 ttatctgttt agggcgtgtg gcggtggact tttatgggca acaaatcggt tcccgtttgg
      121 aagagatgac cacattttca aaatacctcg gcggctcgtc aggcaatgtg gcttacggta
      181 cggcggtgca aggtttacgt tcttcaatgc tggcaagggt cggtgacgaa catatggggc
      241 gttttctacg tgaagagcta caaagcgtag gcgtggacac cagccattta attaccgata
      301 aagaacgctt aactgcactg gttattctcg gcattaaaga taaagagact tttccgctga
      361 ttttctatcg tgataactgt gcggatatgg caatcaccaa agacgatttt agcgaagcct
      421 atattgcttc cagccgttgc ttagcaatta ccggcacgca cctgtcaaac cctaaaaccc
      481 gtgaagctgt gctgaccgca ctcgaatatg cgggtagaaa tgaggtaaaa cgagcgttag
      541 atattgatta tcgccctgtg ttatggggat taacctcact cggtgatggc gaaacccgtt
      601 atatagacag tgccgaagtc accaaatcgc tacaagaagt attgcaccat ttcgatctga
      661 ttgtcggcac agaggaagaa tttcatatcg caggcggttc gaccgatact ctcactgcac
      721 tcaaaaatgt gcgtaaagtc tctaatgccg tattagtgtg caaacgtggg gcattagggt
      781 gttcagtatt tgaaggcgag ctgccgaatg atttagacgg tggcttgaat gtgtacggcg
      841 tgcgtgtaga agtgctgaat gtgctgggtg caggcgatgc ttttatgtcg ggcttactgc
      901 gtggctactt aaataacgaa ggttgggaac aagcgtgcca ctatgccaat gcgtgcggag
      961 cgttagtggt atctcgtcac ggctgttcac cggcaatgcc gaccaaacgt gaacttgacg
     1021 attatttaac ccgtgcagag agtgtaccac gtccggattt agatgagcgt ttaaaccact
     1081 tacaccgtgt caccacccgc aaaaaacagt gggaaaatct ctgtattttt gcttttgatc
     1141 accgcaaaca gttggtggat atggcaaata aagtcggtgc agatcttgct cgcataccga
     1201 aactgaaaca gctgctatta aaagcgatgg aacaaaccgc aactcaatcc ggcttaccac
     1261 aaaatcaagc agggatccta gccgatacca ctttcgggca agatgtactt aatgagatta
     1321 ccggcaaaga ttactggatt gcccgcccga ttgaagagcc gtcttcccgc ccgctggaat
     1381 tagagtatgg agatttaggc tctcagctag catcattccc acgcgatcac gtcatcaaat
     1441 gcttggcatt ttaccacccg aatgatgaaa gcggtttaaa agcccgccaa gatcgcaagt
     1501 taaaagaggt gtatcaagcc tgttgccgta ccggtcacga gctattgttg gaaattattc
     1561 tgcctgctga tatgccacag agtgaggctt tctataacca agcgatagca catttctacc
     1621 aacttggcat taagccggaa tggtggaaat tgccggatgt ttctcgcgca caatggcaag
     1681 cgatttccgc caccattgag gcaaacgatc cggactgtcg tggtatttta attttaggtt
     1741 tagatgcccc gttagaggtg tttaaagaca cctttgaaca ggcaaaaggc tgtgagaaag
     1801 tgaaaggctt tgcagtcggc cgcaccattt tcggtgaacc gtccgaaaaa tggcttgccg
     1861 gtgaattgga cgacaatacc ttgatttctg cggtatcgga gaaatatcgc actttaatcg
     1921 atctctggca acaacgttaa tcatattgct ttaagtgaaa accccttagc ttatgatatt
     1981 aagcaagggg ttttgttttg caaaaaatcc ggctaaacag accgcttgct agccgtaaaa
     2041 atgcccgaat tttcctaaaa aagcgaatat cgggcattcc tcaatctgct tatcacaaaa
     2101 taagtgtgat gaagttcaca tttcatacat aaaccccccc ctttttatcg aaacaaattg
     2161 ttttattttt atcaaaatgg aaaatgtatt tcagtttagt tctatttaga ttcaatagga
     2221 gtctgtgtat gagttgcaac acttcaccaa aacgtttcca cgccagttgg gagtcgatca
     2281 aacagcacca agtaccagag tggtatgaag atgctaagtt tgggattttt attcactggg
     2341 ggatttactc cgtacccgcc tttgccccac cgacctgtca gttaggtgaa attgatattg
     2401 atgaacagtg gttctgtaat aacccttatg cagaatggta ttttaattcc atcaatgtca
     2461 aaaaaggccc gacctacgaa catcatatca aaacttatgg cgaatccttt gcctataacg
     2521 acttcctgcc actatggaaa gcggaaaaat ggcaaccagc agaatgggcg aaactgtttg
     2581 ccaaagctgg cgccaaatat gtggtattaa ccaccaaaca tcacgatggt ttctgtctct
     2641 atccaagtaa atataccgat ttcaactgta tgcaagccgg accgaagcgg gatttaatgg
     2701 gcgagctgac tgaaaatgtg cgggagcaag gcttaaaaat gggcgcttac tactcgggca
     2761 ttatcgattg gacttattcc tctgccccga ttttcaccga aagtcaaaac ttctcaaacg
     2821 cttgcccgac ttacgaatac gctgactacg cctataaaca agtggtagag ctgatcgacc
     2881 gctataaacc agatgtattg tggaatgata ttggctggcc aaaagtgggc gagcatatgt
     2941 taccacacct atttgctcat tattacaaca gcgttcaaga gggcgtagtg gacgaccgct
     3001 ggaacaaact atggtgcgat ttcacctcca aagaatacaa acacggcgtg gcttcccgtg
     3061 ataagaaatg ggaaatgtgc cgtggtatgg gcttatcgtt cggctataac caggtagaag
     3121 acgaaagcca tttgatttcg gtgaaagact tgatttccct attggtagaa accgtagccg
     3181 acaacggcaa tttattgctg aatatcggcc cgaaagcaga tggcactatt ccgcaagagc
     3241 aagttgaacg cttattagca ctggggagct ggctggaaat aaatggagag ggaatctata
     3301 aatcccgttg tagccaacac caaaccgagc aaatcggtga tgtaacctat cgttatacca
     3361 aagttggggc ggatctctat ctttacattg atggcttaac cgaagcccaa atcagcttgc
     3421 cactgtcagt ttatggtgag ctacaagcac tcaataccga gctgaaattt accattgaaa
     3481 acaccgcaca aggcagagtg ttacatattc aaaactacca gccggattgg tatgtattag
     3541 gctttaaaat tgcagatggg gagaaatagg tatgtccgtt tcacaacgtt ttattgactt
     3601 tatgaatgcc cacgttgctc cagtagcacg tcgaatggaa aatcaaccgc atatttccgc
     3661 tattcgagac ggttttattg tagtgttacc attcttaatt gtcggcagct ttattatgat
     3721 tttgctaatt ccgccatttg atgaaaatac ccaaaatgct ttcgggcaag cttggtggcg
     3781 ttttgcgaat tgggcaagcc cttacggttg gaatttcttc caaatgtcgt ttaatgcgat
     3841 ttcattattt acctctgcca gtatcgccta caaccttgcc aaagcctata aaagagagcc
     3901 cctgccagcg gcatttctct cggtaatggc atttttgtta gtggctgcac cggtcaaaga
     3961 cggaatgatg gatattaaat tctttggcgg tattgggcta ttctctgcga tttttattgc
     4021 catttattcg gtagaaatga cccgcttgtt ggagttctta aaaattaaaa tccgcttacc
     4081 gaaagaagtg ccgcacgctg tagcagaatc tctgaatatc gtgattccaa ttttagcgat
     4141 tttactgact ctctatccgt ttgccatttg ggtggaaagt gccacaggtc gtaatattcc
     4201 acaactaatt atggacttta tggcgccatt gattgctgtg agtgactcgc taggggcaat
     4261 ttgcttgttc gttattgcta cccatttgct ctggttctta ggcattaacg gctcattggt
     4321 actaatgcaa ctttggacac cattcctact gcaaaatatg gcggcaaact tagcagcatt
     4381 ccaagctggt gaaccgctcc cctttatcat caccaattcc ttctgggatt tctatatcgt
     4441 acacggggca agtggtggtg taatagcctt ggcattcctg ctggtacgca gtaaatcggc
     4501 acacttacgc tctatcggta aaatcggctt ggttccgtcc ttcttctcta tcggtgaacc
     4561 aattgtgtat ggtgtgccaa tggtggttaa tccgctgttc tttatcccgc tgattttcgc
     4621 accactagcg aatgcggtta ttgcctacct gattttagat tttaacctta ttcaccgtgt
     4681 ttacttgatg gcaccgtgga caacaccggc accgattggg gcatatctgg tttctgccgg
     4741 tgatatttgg gcaccaattt taagtattgc actgattatt ttggatattg tgatctacta
     4801 tccattcttc aaaatgtatg aaaaagtctg cgtcgagaaa gaaaaagcac aaaatctaga
     4861 tgaaaaagca caacaggagt tgctagaaaa agcacgaatg gcagcacaat aacacttatt
     4921 tctagcgtaa ttgagagggg ctaatcgaaa gattagccct tatttttgca aaaaattgac
     4981 aaaatttatc cgcttgtaaa caagaaaagc gtataatgaa ataaaaaatt caagcagaga
     5041 taatttgaaa tgaatggacc tactcaatta gcacattttc aagaacaaat ccgaaaagag
     5101 tatgacggac taagtaaacg tttaaaacaa gtggcacagt atgtgctgga taatggcaac
     5161 agcgtggtgt ttgatacggt ttctaccatt gctgaacgag ctgatgttcc cccttcaacc
     5221 ttaatccgtt ttgcgaatgc gtttggattt agcggtttta atgaaattaa acagctttat
     5281 cgccaagata tgatggaagg cacatcacgt tacaccgaac gtgtgcaact ttttcgtcaa
     5341 attgaaccgc ttgatgagca aaacagtgag ctcaatcata ttttagatgt gttcgtacag
     5401 ggtaatacgc aggcactgca acagttatcg acacaagtca gcgaagagca gttagcacaa
     5461 acggtagata ttttacacaa tgccaaacgc atttttattg tgggtttaaa acgctcattc
     5521 agcattgcga actacctaaa ctatgcgttg caccatttgg actatgatgt ctttatgatc
     5581 gatggttcgg gcggtatgtt tgacgagcaa ttaagccgaa tccgtgaagg tgatgcggtg
     5641 attgccgtga gcttttcccc gtatgcgaat gaaacactca acgtggtgac agcaactgcc
     5701 agaccggggg taaaacatat tgcgatcact gatagccaac ttagcccact cctttcattt
     5761 agcgatgtgt cgtttattat taaagaagca caggtgagtg gttttcgatc ccagtgtgcc
     5821 acaatgacgc tcgcacaaac gattgccgtt tcactggcgt tgaaaaaagc ctagattaag
     5881 aaagtgaaag acctgcgtaa atacaggtct ttgtctatta accgattaaa gcgttaattt
     5941 catcacgata ctgcccggct tttgcaccaa aaataatttg ttgcgtatca ccgactttaa
     6001 tgacatcaac cgccccaagc tttttcagtg ccgacttatt taataaaccg ttgttatgta
     6061 acaccacacg taaacgggtt aaacaagctt caacttgctt gatattctct ttgtttccta
     6121 aagcattaat aatttctaga atgatagatt
//
LOCUS       DANLXQ010000144         6095 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.144, whole genome shotgun sequence.
ACCESSION   DANLXQ010000144 DANLXQ010000000
VERSION     DANLXQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.144"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..223)
                     /locus_tag="PXD94_002143"
     CDS             complement(<1..223)
                     /locus_tag="PXD94_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286964.1"
                     /translation="MSTTLNEINITELDAGPEIKSLSLNDAVNSFLNDSACPICGKNC
                     QYWQRLKRSLNRKENQGKGDKVLVKNPSPI"
     gene            complement(257..1543)
                     /gene="purD"
                     /locus_tag="PXD94_002144"
     CDS             complement(257..1543)
                     /gene="purD"
                     /locus_tag="PXD94_002144"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL6286965.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            1777..2151
                     /gene="rpsF"
                     /locus_tag="PXD94_002145"
     CDS             1777..2151
                     /gene="rpsF"
                     /locus_tag="PXD94_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL6286966.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            2126..2500
                     /gene="priB"
                     /locus_tag="PXD94_002146"
     CDS             2126..2500
                     /gene="priB"
                     /locus_tag="PXD94_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL6286967.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            2475..2702
                     /gene="rpsR"
                     /locus_tag="PXD94_002147"
     CDS             2475..2702
                     /gene="rpsR"
                     /locus_tag="PXD94_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL6286968.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            2718..3167
                     /gene="rplI"
                     /locus_tag="PXD94_002148"
     CDS             2718..3167
                     /gene="rplI"
                     /locus_tag="PXD94_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL6286969.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(3231..4007)
                     /gene="dnaQ"
                     /locus_tag="PXD94_002149"
     CDS             complement(3231..4007)
                     /gene="dnaQ"
                     /locus_tag="PXD94_002149"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL6286970.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(4095..5765)
                     /gene="ettA"
                     /locus_tag="PXD94_002150"
     CDS             complement(4095..5765)
                     /gene="ettA"
                     /locus_tag="PXD94_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL6286971.1"
                     /translation="MSTQFVYTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGA
                     GKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNAL
                     TQLDEVYAMYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAK
                     IEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITH
                     DRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEW
                     VRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSY
                     GDRTLIDDLSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASV
                     DQFRDAMDDKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGE
                     RGRLHLAKLLQRGGNVLLLDEPTNDLDVETLRALENAILEFPGCAMVISHDRWFLDRI
                     ATHILDYGDEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
BASE COUNT         1605 a         1404 c         1285 g         1801 t
ORIGIN      
        1 aaattggaga tggattcttc acaagaactt tatctccctt accttgattt tcttttctat
       61 ttaaagatct ttttagtctt tgccaatatt gacagttttt tccacatata gggcacgcag
      121 agtcatttag aaaagagttt actgcatcgt ttaatgatag tgatttaatt tcggggcctg
      181 catcgagttc agtgatatta atttcattaa gtgttgtact cataataaat ttttccttgt
      241 tttaagagtt tataaattat ttcgcttgtt ttcttgccac tgctcggtag ccaatatctc
      301 ggcgatagaa tttgccttgc cattgaattt gttcggcaag ttgtaaggct tgttgttgag
      361 cttcaaatac actgttgcca agtgcggtgg cacaaagtac acgtccaccg ttggtgagca
      421 gttttccttc tttttgctca acccctgcta agaaaacttt ttgattggag gtagaggtcg
      481 gaatgcctat gatttcatcg cctttacggt aatcgcccgg gtagcctgcg gcagctaaaa
      541 caatgcctaa tgcggcttgt tcacaccatt ttgattgcac gctatccaat ttgccttcgc
      601 acgcttttaa gcacaattca acgaggtctg attccaaacg catcataatc ggctgagttt
      661 cagggtctcc aaaacggcag ttaaattcaa tcactttcgg ctgaccgttt ggcataatca
      721 ttaagccggc atagagaaaa ccggtgtaag gggtattttc gcttgccatt ccttgcacgg
      781 tcgggtaaat aatttcgtcc attacacgtt ggtggattgc ctttgttacc accggagcag
      841 gggagtaagc ccccattccg ccggtattta agccgctatc gccctcgcca acacgtttgt
      901 ggtcttgtga ggttgccatt ggttcgacat ttttgccgtc caccatcaca ataaaactgg
      961 cctcttctcc gtctaaaaat tcctcgatca ccacacggct gccggcttca ccaaaggcgt
     1021 tgcctgacag catatcacgc actgcatctt ccgcctcttg taaagtcatt gcaacaatta
     1081 cgcctttgcc tgcggctaaa ccgtctgctt tgattacaat cggtgcacct ttttgtttta
     1141 agtaggcaag tgccggttca atttcggtga aattttggta ttctgcggtt ggaatctgat
     1201 ggcgagccaa gaaatctttg gtaaatgctt tagaaccttc taattgagca gcggcttggg
     1261 tcgggccgaa aatggttaaa ccgtgggctc gaaaagcatc aaccacgcca actactaacg
     1321 gtgcttcagg gccgacaatc gtcaaaccta cgctattttg tttagcgaat gtaactaagg
     1381 caggcacgtc agtggcggaa atagctacat tttcaatgcc gttttccatc gctgtgccgg
     1441 cattgccggg ggcgacaaag actttgtctg cgagcggcga ttgacgaact ttccaagcaa
     1501 gagcgtgttc acgcccaccg ttgccgataa ttaaaatatt cattgggatt ccttgcaaaa
     1561 atgataaagc aaacgtttgc gtattgtact tgatttgttt atctgctgac aagcagtcgt
     1621 ttttctcgga ttttttgcaa aatacgaaat tgttgcttgc aaaataggct tttttcagta
     1681 taatcctgcc gttcagtcac attcgttttt taatccttgc ttccacaagt tggtgactgg
     1741 ctaaagttgg aggctgctaa cccgtaagga tagtcaatgc gtcactacga aatcgttttt
     1801 atggttcacc cggaccaaag cgaacaagta ccagcaatga ttgagcgcta cacagcgtct
     1861 gtaaaagaag ctggcggtca agttcatcgc ttagaagatt ggggtcgtcg tcaattagca
     1921 tacccaatca acaaacttca caaagcacac tatgtgttaa tgaatgtaga agcacctcaa
     1981 agtgtaatcg acgagttaga aactaacttc cgttataacg atgcagttct tcgtaactta
     2041 atcgttcaca ctaaagcggc agtaactgaa gcgtcaccaa tggtgaaagc gaaagaaagc
     2101 aaagttgctg aagcggtagc tgaagttgaa tcagaggaag caggcgagta atttgccgat
     2161 tgataattgt ttaatcttaa gcggaacggt ttccagcttg gttaaacaaa gcctaaaccc
     2221 ccttggtgtg cctcattaca gtttttggtt agaacatcgt tcaatccaaa ccgaagtgaa
     2281 tttagaacgc caagcgtggt gcaaaatcca agtggttttg gcaggcaatc aatttagtta
     2341 tttaacccaa caaattaagc tcggtgagag cgtgagaatt aaaggtttta tccatactca
     2401 caaagacttt aacggtttaa atcagttagt cttacacacc gaacaaattg aatttataga
     2461 tcaggagaag ccaaatggca cgttatttcc gtcgtcgtaa gttctgccgt ttcacagcgg
     2521 aaaatgttgt tgaaatcgat tacaaagata tcgctacatt aaagaactac atttctgaaa
     2581 gcggcaagat tgttcctagc cgtattactg gtactcgtgc gaagtatcaa cgtcaattag
     2641 ctcgtgcaat taaacgcgct cgctaccttg cgttacttcc atatactgat aaccaccaat
     2701 aatcaggaga gagtacaatg caagttattt tattagacaa agttgctcac ttaggttctg
     2761 tgggcgacca agtaaccgtt aaatctggtt ttgctcgtaa cttcttaatc ccacaaggta
     2821 aagcggttat ggcaactgcg gcaaacattg ctcactttga agcacgtcgt gcagaattag
     2881 aagcaaaagc tgcagcagca ttagcaactg cacaagaacg tgcagcgaaa attgcagcat
     2941 tagcagcagt atcagtatct gcaactgcag gtgatgatgg tcgtttattc ggttctgtat
     3001 cagctaaaga catcgctgaa gcattaactg cagcaggtgt tggagttgct aaatctgaag
     3061 ttcgtttagg tgaaggtcca cttcgtacaa ccggcgaaca ccaagttaaa attcacttac
     3121 accctgaagt gaatgcaact gtagcagtaa acgttgttgc tgaataatca gtagaaatag
     3181 aaaacccagc gaaagctggg ttttttgttt gctttattta ttggttagaa tcagtgagat
     3241 atttctgctg tgtcggttaa tgcttttgtc cataggcaat ttcctttgga ttttttctca
     3301 atcagtttca gaagtgctaa atgcgactct tcctcttcat cgtttgcttt aagaataatt
     3361 gcctcatcgg catttaacac aattgcagaa aattcttctt caatatcaat aacttcactt
     3421 accacttcag tttcttcttc gcctaataat gaaatctgac cgccggtcat cattaggaat
     3481 acatcagcta agatctccgc atccagtaac gccccgtgta gtacacgctt gctattatca
     3541 atgccgagcc tgtcgcaaag tgcatctaaa ttattccgtt tgccggggta cattcttcgt
     3601 gccatttgca ggctatcggt tacggtacac atttctgcgg tttttgccgg tggattaggc
     3661 agataagaaa attcttgatc cataaacgcc acatcgaagg gggcattgtg aataattaat
     3721 tccgcacctt tgatgaaatc caaaaactcc tcggcaattt cagcaaaaac aggtttatct
     3781 tgcaaaaatt cattggtgat gccgtgaact gcaatcgcct cttcatctac ctcacgcggt
     3841 ggcttgatat aaacgtgata agttctgccg gttaaacggc ggttaatgac ttcaaccgca
     3901 ccgatctcaa taatattatg cccaatctgt ggcgggccac ctgccatatt cattcctgtg
     3961 gtttcagtat ccagcactac ttggcggata attttttctt ctgtcatatt caatttaaaa
     4021 ttgtaggggt gaaatatttt tatcccttaa ttctgcattt gattaggggc gaaacatatt
     4081 tcgcccctac attgttactt cgcaatccgt ttatatttca ctcggtgcgg ttgggtggcg
     4141 gcttcaccga aggttttctt cttccactct tcatagtcgg agaagttacc ctcgtagaac
     4201 gtcactttgc cttcatcgcc gtagtctaaa atatgggtgg cgatacggtc taagaaccag
     4261 cggtcgtgcg aaattaccat tgcacagccc gggaactcta agatcgcatt ttccaacgca
     4321 cgcagggttt ctacgtctaa gtcgttggtc ggctcgtcca gtaacaacac gttaccgcca
     4381 cgttgtagta gcttcgccaa gtgtaaacga ccacgttcac cgcctgaaag ctcgcccaca
     4441 cgtttttgct gatctacgcc tttgaagttg aaacgcccca cataagcacg gcttgggatt
     4501 tcaaagttac caatcgtgag aatatcctgc ccgttcgaca cctcttccca cacggttttc
     4561 ttatcgtcca tcgcatcacg gaactgatcg accgatgcca ttaccaccgt ttcaccaagc
     4621 acgattgaac cagaatccgg ctgttcttta ccagaaagca tacggaacaa ggttgattta
     4681 cccgcaccat ttgccccgat aatcccgaca atcgccccct tcggaatagc gaacgacaag
     4741 tcgtcaatca aagtgcgatc gccgtaagat ttagtcaggt tttgtacctc gatcacttta
     4801 tcacctaagc gtggacccgg tggaataaag agttcgttag tctcgttacg tttttgatat
     4861 tcgcctgagt taagctcttc aaagcgtgcc atacgagcct tacttttcgc ttggcgacct
     4921 tttggatttt ggcgtaccca ttccaactct ttctcaatcg atttttgacg agccgattcc
     4981 gccgcttgct cttgtgccaa gcgtttttct ttctgctcca accaagaaga gtagttacct
     5041 tcccaaggaa tgccttcacc acggtcaagc tctaagatcc agcccgcaac gttgtctaag
     5101 aagtaacggt cgtgggtaat tgccactacg gtgccttcgt agtcgtggag gaaacgctcc
     5161 aaccacgcca ccgattccgc atctaagtgg ttggtcggct catctaacaa taacatatcc
     5221 ggtttttcca gcaacagacg gcaaagtgcc acacggcggc gttcaccccc tgataaatgc
     5281 tcgatttttg cgtcccaatc cggcagacgt aacgcatcgg cagcacgctc taactggttt
     5341 tgaaggttat ggccatcgtg ggcttgaata atcgcctcta attccgcctg ttttgccgct
     5401 aatttgtcga aatcggcatt ctcatcagca tacatcgcat agacttcatc aagctgggtt
     5461 aaggcgtttt tcacttccgc aaccgcttct tccaccgctt cacgcacggt ttgctgtggg
     5521 gcaagtttcg gttcttgtgg aagatagccg attttaattc ccggttgtgg acgagcctcg
     5581 ccctcgaact ctttatctac gcccgccata atgcgtaaaa gcgttgattt acccgccccg
     5641 tttaagccga gcaccccgat tttcgccccc gggaaaaagc tcagggaaat atctttaaga
     5701 atgtgacgct tcggcggcac gactttgccg acacggtgca tcgtatatac aaattgagtt
     5761 gacattggtt gtattcctca caaaaaaatt cgccttattt tacttgaatt tacagccaaa
     5821 atatagggtg gttttggctt ttggtaaagt aaccttttcc aatagagatg ttatttgagg
     5881 ctgcatagta ctatgcattt ctcttacctt gacaggtaag agaaacaaat cctgaaggaa
     5941 aaaaatttta aggaagttat tctatgcaat atgtatgtcc aaaaacactc aaattattca
     6001 acagcattgt tggttgttat aagggagaag attaattctc tacctcatca aggcttgatt
     6061 ttagatttaa tatccatttc taaagatgat ttaga
//
LOCUS       DANLXQ010000145         6095 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.145, whole genome shotgun sequence.
ACCESSION   DANLXQ010000145 DANLXQ010000000
VERSION     DANLXQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.145"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            124..336
                     /locus_tag="PXD94_002151"
     CDS             124..336
                     /locus_tag="PXD94_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL6286972.1"
                     /translation="MSAITGTVKWFNATKGFGFITPDQGGKDVFVHFSAIEANGGYRT
                     LEDGAKVQFEIQDSERGASAVNVKTI"
     gene            complement(396..1550)
                     /locus_tag="PXD94_002152"
     CDS             complement(396..1550)
                     /locus_tag="PXD94_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL6286973.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAAALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(1547..2365)
                     /locus_tag="PXD94_002153"
     CDS             complement(1547..2365)
                     /locus_tag="PXD94_002153"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL6286974.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGAAKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            2442..3350
                     /gene="rdgC"
                     /locus_tag="PXD94_002154"
     CDS             2442..3350
                     /gene="rdgC"
                     /locus_tag="PXD94_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL6286975.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQVVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            3371..3976
                     /locus_tag="PXD94_002155"
     CDS             3371..3976
                     /locus_tag="PXD94_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL6286976.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            3992..4783
                     /locus_tag="PXD94_002156"
     CDS             3992..4783
                     /locus_tag="PXD94_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL6286977.1"
                     /translation="MKTEWIKHLIQQLTTLSKAIENYFTSGSFGVKFYPFLMHPCFRK
                     SCAFLIFIIAMVSSFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKI
                     SELSPLSVVMRSMFVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGI
                     QPSSRGEFGIYNLGASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILI
                     TIFLPTLFSPLIERAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
     gene            4787..5929
                     /gene="argE"
                     /locus_tag="PXD94_002157"
     CDS             4787..5929
                     /gene="argE"
                     /locus_tag="PXD94_002157"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL6286978.1"
                     /translation="MKQIPFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSRNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
BASE COUNT         1793 a         1399 c         1292 g         1611 t
ORIGIN      
        1 gtgcgttaat taaagttgaa tcttgttagt tattttcctc aaggcaagtg attgatagat
       61 acgtctgtaa tatcccgttt agctatcttt tatcactttt tgtatttata taaggaaaat
      121 tttatgtcag caattaccgg cacagtaaaa tggttcaacg ccaccaaagg ttttggtttt
      181 atcacccctg atcaaggtgg caaagatgta ttcgtacact tctctgctat cgaagcaaac
      241 ggcggctacc gcacattaga agacggtgct aaagtacaat ttgaaatcca agattcagag
      301 cgtggagctt cagccgtaaa cgtgaaaaca atctaatttc agcaaattca atgaatttcg
      361 ataaaaatgc cgacaacttg tcggcatttt tgctatcaat taatctgtac ttcaaacctt
      421 ttcggcacaa taaaaatggc aggtagggta aaaattgcca tcaaccaaaa tgtcaggctt
      481 ggggaatgtt gatagaccat tccggcaata aaggtaaata ctgccataaa agcacagctt
      541 gccaagccaa aatagagacc ttgtaatttc gtcacacgct ctgccgattg catcgaaata
      601 tagcgaatca tcgcaatatg agccgccccg aaggtaatag aatgtagcat ttgcattaca
      661 ataatcagca cgatctctgt agtactaccc attattgccc aacgaatcac tgcaaaaacg
      721 gcagctaaaa tcattaaatg gctgattttc cacgatttaa acaagcgttt agagaccaaa
      781 aagaacaaaa tttccgccac caccgccaag ccccaaaaca ggctagtcat tgcggttgaa
      841 atactggcat ttgaccaatg aatggtgctg taagtgtaat aagcggcgtg cgaggctgaa
      901 attaacgaca ccgcgatcaa catttttaag gtttccggct ctttcaataa ttggcgataa
      961 gttacgcttg aagtattggc tttattacct tgttcttcaa acccgacttt gggcgtagca
     1021 agctgcccta ttcctaaaaa aaccaagaaa ccgatgataa tccacaccac cgaattttca
     1081 ccaagcaagc cgataagata gccggcagaa agtgagccta ccacaaacgc cattgagccg
     1141 aataaccttg ctctgccgta atccagcccg atctgatgtt gccaaattga ggcaatgcta
     1201 tcggctatcg gcattgcccc cgaattgaac atttgaaaca acattaacac cggaaaaagt
     1261 agccaaatga aatcccctga aaacagcaac gccgccgcag ccgcaatatt cagccaagtt
     1321 aaactgcggg cggtgggaat gagttggctc acggttttta cccgttgtga ggcaagcata
     1381 ctgccggcaa agcggaacaa atagccaacg gcggcaaata agccgatcat ttcagtcgaa
     1441 tagccgtgat gtttaagcca cactggcaaa aacggcaaca acacgccata ggcacaaaaa
     1501 aagccaaagt aattaaatgc cgcccattgg aacggggaaa ctttcatcat aacgattgct
     1561 ccatttctct ggctcgatga attgccgccc acattgcatc atcaacggtt ttagcaagat
     1621 cgtgctgctc aaacacctca agggctttcg ccgttgtgcc tccctttgag gtcacatttt
     1681 cccgcaaggt cgaaagtggc gtagtcgggt tcgcttccac caatttcgcc gcccccaatg
     1741 cgacagattg tacaagcgtt cttgcgtcct gctcggaaaa gcccatttgc accgcacttt
     1801 gctgcattgc ctccataaaa cggaagaaat acgccgggct tgagccggtt atcgcaataa
     1861 tgtggttgat ttgctcctcg gtttccaccc agtaacattt accaacggct gacatcaact
     1921 gttcggcaaa ttggcaagcg gtcggattta ccgatttttt tgcaaataat ccggtcattc
     1981 cttcgctgat taaagacggg gtgtttggca tcgtgcgaat gatatttttc gcactcggca
     2041 acagcgtttc caagcgttgc acagaaatac ctgctgctac tgaaatcacc catttgttgg
     2101 aaaaatcaac atcggcaaac tcggcacaca cttgtgccat catctgtggt tttaccgcca
     2161 gcacgatcac gtcagcctgc tctaccgcct cacgatttgc aaaatttgtc gcaattccga
     2221 ccgcttgtaa ctcctctcgg cgagccaaat tacttttatt gcacgcaatg atttgttcgg
     2281 cagaataacc gctttttaat aagccttgaa taatcgcata cgccatattt ccggtgccga
     2341 taaaggcaag ttttttctcg ttcattcgca aattctctac tgtgttgggt tgaggtctgt
     2401 taaaataaca ctattctaca cccgataaag gatatgcaaa aatggtttgg ttcaaaaatg
     2461 tgatgatcta taaattaacc ggcgagctgg cgttggaaag caatagcctt aacgagttac
     2521 agcaagaaac ccgctttacc ccttgcagcc agcacgatat gagcaaattc ggctgggcta
     2581 cgccacttac cggctcggat ttactgcatt tctcacaagc gaaccaattt ttgctgattt
     2641 cacacaaaga agataaatta ttacctgcca atgtggtgaa agccgaaacc gagcaacgca
     2701 ttgcagaact ggaagaaaaa gaggctcgta agctgaaaaa aaccgaaaaa caagccgtga
     2761 aagatcaggt cgtttctgcg ttattgcctc gagcattcag caaacatcaa ttcaccgctc
     2821 tttggttgga tttagataat cagttggttt atgtggatgc agcctcgagc aaacgggcgg
     2881 aagatacgct ggcgttactg cgaaaaacac ttggctcgtt gccggttgtg ccgatttcct
     2941 ttaccctacc gccggcagag gtaatgacga attggatcgc caaagggcat acgccaagtt
     3001 ggttgaattt gttggaagag gcagaactga aatctttcga taccgacagc attattcgct
     3061 gtaaacgcca agatttagag acggaagaaa tcgcccggca cttgcaagcg ggcaaattta
     3121 tcacaaaact tgcgattgaa tgggaagggc atttcaattg cgtgttaaat gaagacgcca
     3181 cgctttcacg cctgaagttt gccgatgaag tgcgagagaa aaatgacgat attctcaaag
     3241 aagatgtggc tcaacgcttt gatgccgatt tcttattaat gaccgaagag ctgaaacttt
     3301 tcaccgaaaa aatgattgcc gaatttggcg gaattaaatc cagaatttag aaaaaaattt
     3361 aaggaaagca atgttagatc aaaccattat cgaccaatat gacgccttga tttttgatat
     3421 ggacggcaca ttaattgaca cgatgccaag ccacgccaag gcgtgggaaa aggtgggcga
     3481 agtgttaggc tacccaatca atccaaagcc aatgtacgaa ctgagtggct caaccacttt
     3541 tgtgattgcc cgagaaataa tgcaacgctc caatatcccc gagcattatt ttgagcaagt
     3601 ggtacaacta aaacgggaat ttgggattga aatggtgtta gccaatgcca ctctgttgcc
     3661 tgcctttgag attattaaag ctaacgttgg caaaaaacca atggcaatcg gcacaggctc
     3721 acaccgtgca atggtggaat tattagacca aaaatttaac ttacgtcaat atgtttcggt
     3781 tattgtagat tctgatgatg taagcaacca taagccagcc cctgaaacct ttttaaaatg
     3841 tgcggaaaaa cttggcattg ccccacaccg ctgtttggtg tttgaagatg ccgatttagg
     3901 cgtgcaagcc gccctcaacg gcaatatgaa cgtgtttgat gtacgtacga atacgataac
     3961 tcactcaacc cgttaaaaat gataggttta tatgaaaaca gaatggatta aacatttaat
     4021 tcaacaatta accacgcttt ccaaagccat cgaaaactac tttaccagcg gttcatttgg
     4081 ggtgaaattc tacccttttc taatgcaccc ttgttttcgt aaaagctgtg cctttttgat
     4141 ttttattatt gctatggttt caagttttag cgggctattt tatttggtgg cggaacacat
     4201 cacgatttta tttagcgaaa gatcgctcac cacttacttc caccatagca cccttgaatt
     4261 gatgattcca gtcggcacaa tgatagatga aaaaatcagc gaattatcac cactttcggt
     4321 ggtaatgcgt tcaatgtttg tgctacaagc agtcgttttt ttctttattt atgcaatcgg
     4381 catcacccac attgctcaca acaaattgcg ggtcatcggc ttattgcttt cgctatgctt
     4441 tgcgattggc tgctcactag ttgccggtat tcagccaagc agtcgtggcg aattcggcat
     4501 ttacaattta ggggcaagca ttacgttttt aatcggcaat ttaacgctga tcatcgcagg
     4561 cttcgatctc taccaccctt caatgcaatt ttttaaacgc tttagcattg gtgcgggcgt
     4621 tatcggggca atctgtattt taatcacgat tttcctacca accctgttta gcccgttaat
     4681 tgaacgagca ggtatttata ccattatgct gtgggaaatt ttagccggtt ttgccatcat
     4741 taaacgcctg ttgaaattgc cctcattcag acaacaagga taaccaatga aacagattcc
     4801 ctttattcaa cgctatgctc aactgattga gctaccgacc atttccagcc ttgttgccga
     4861 agaagattta tcgaaccgcc gtttgattga attgctcgcc acttggctgg cagatttcgg
     4921 ctttaaaacc gagattttag cggtagaagg cagccgtaat aaatacaatt tacttgcgac
     4981 ctatggcgag ggtgagggcg gcttattatt ggcagggcat accgacaccg tgccgtttga
     5041 tgaaggaaaa tggcgcttca atccattcca attaaccgaa aaagcaggca aactctacgg
     5101 cttaggcacg gcagatatga aaggcttttt cgcctttgtg gtggaggttg tgagccaact
     5161 cgacctaacc caaatcaaaa agccgattcg cattcttgcc accgctgatg aagaaaccac
     5221 aatgctgggg gcaagaacct ttgctcagca cagccacatt cgccccgatt gtgcgattat
     5281 tggcgagccg acctcgctca aaccgattcg agcccacaaa gggcatattg gcgaatcggt
     5341 tcgcattacc ggcagaagcg gacactccag cgaccccgcc aaaggcatta atgcgattga
     5401 attaatgcac caatctatcg gttatttaat gaaaatgcgt gatgaactgc gagagaaata
     5461 ccacaatccg ctgttccaag tcacccaccc gacaatgaac ttcggcaata ttcacggcgg
     5521 cgatgccatt aaccgcattt gtgcctgttg cgaattgcaa tttgatatgc gaccactacc
     5581 gaatatggcg gtgcaagatt tgtatgcaat ggtaaacgag catttaaaac cgatgctgga
     5641 acaatatggc gatttaattg aaatccgcca tttacacgat ggaattccgg gctacgaatg
     5701 cgaacactcg gcacaaatcg tgcaagtggt tgaaaagcta ctgggcgaaa aatgcgatgc
     5761 cgtcaattac tgcaccgaag ccccattcat tcaacaactc tgcccaacgc tggtactagg
     5821 ccccggctcc attgaacaag cccaccagcc cgatgagttt ttggaaatga aatatatcga
     5881 accgacaaaa gagttattga ctaaattgat ttatcatttt tgtggataaa tcaacagaaa
     5941 caagcgctca ttttcgccat actttttgca aaagaatcgg caaaaatgac cgcttgatat
     6001 ttagtattga gtgacctata aacttccata cttcatcatt gatttaagga gattctgtta
     6061 gataggtatt aatctctagc taggtcttgg ataca
//
LOCUS       DANLXQ010000146         6041 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.146, whole genome shotgun sequence.
ACCESSION   DANLXQ010000146 DANLXQ010000000
VERSION     DANLXQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.146"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(88..1452)
                     /gene="mpl"
                     /locus_tag="PXD94_002158"
     CDS             complement(88..1452)
                     /gene="mpl"
                     /locus_tag="PXD94_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL6286979.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTDHILVMSNGAFGGIHQKLLAK
                     LADK"
     gene            complement(1748..2503)
                     /locus_tag="PXD94_002159"
     CDS             complement(1748..2503)
                     /locus_tag="PXD94_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL6286980.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
     gene            2658..2975
                     /gene="metJ"
                     /locus_tag="PXD94_002160"
     CDS             2658..2975
                     /gene="metJ"
                     /locus_tag="PXD94_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL6286981.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            3023..3253
                     /locus_tag="PXD94_002161"
     CDS             3023..3253
                     /locus_tag="PXD94_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL6286982.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            3246..4670
                     /gene="panF"
                     /locus_tag="PXD94_002162"
     CDS             3246..4670
                     /gene="panF"
                     /locus_tag="PXD94_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL6286983.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYATGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(4747..5937)
                     /gene="nhaA"
                     /locus_tag="PXD94_002163"
     CDS             complement(4747..5937)
                     /gene="nhaA"
                     /locus_tag="PXD94_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL6286984.1"
                     /translation="MEKLKEFLKSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTI
                     VSVQFGEFALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVG
                     GMIVPAIIYWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAI
                     IDDLGAIVVIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSV
                     LKSGVHATLAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLL
                     EMGMEQLSSPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLC
                     GIGFTMSMFLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPEN
                     NATY"
BASE COUNT         1735 a         1364 c         1220 g         1722 t
ORIGIN      
        1 ttttgaattt ttcgatgcag atcacaaaaa tagagagtta tacaaacaag cggtaaaatt
       61 ttgtaaaaaa tttaccgctt gtaaaggtta cttatcagct aatttggcaa gtagtttttg
      121 atgaataccg ccaaaggctc cgttgctcat cactaagatg tgatcggtgg gtttggcttc
      181 tttgacgact agatcgacta aatcatcaat atttgccgac caatacgccg gttgcgataa
      241 cacattggta atctcgctca cactccacgc aataatatca ggctggtaaa caaacactgc
      301 ttcggcatcg tgtaggcttg gggctatttc atctttatgg acacccattt tcattgtatt
      361 tgaacgaggc tctaacactg ccaaaatgcg ttgatttttg ccgactttac cacgcagagc
      421 atcaatagtc gcttctatcg cagttgggtg gtgagcgaaa tcatcataaa cggtaatgcc
      481 gtttacctcg cctttcactt ccaaacggcg gttagcattc acaaacttgc ctaatgcctc
      541 gcaagcccct tgcacttcaa cccctgcgtg atgagcggcg gcaatcgcca tcagagcatt
      601 atgcatattg tgcttgccga taatcgacca atgcacctcg ccttgtttaa cgcctaagtg
      661 aaacacctca aactgtgagc aatcggcatt taacggttta gcaaaccatt cgttctgttc
      721 gccaataaat tgaagctggc tgtaactgcc catttttagt gtatcttgca cgttttgatc
      781 agcgattgcc gacaaaatac agccactttg tggcatcgtg cgaacaaggt ggtggaactg
      841 gcgttgaatc gcttttaaat catcaaaaat atcggcgtgg tcaaattcca tattattgat
      901 aatcagcgtt ttcggggtgt aatgcacaaa tttagaacgc ttatcaaaga acgcagaatc
      961 gtactcgtcc gcctcaatca cgaagaatgg actgttaccg gcacgagccg aaatgccgaa
     1021 attaccggca acaccgccaa ttaaaaagcc ggtatcaatc ccattttggt ctaaaatcca
     1081 cgctaacatt cctgttgtgg tagttttgcc gtgcgtgccg gaaaccgcca gcacccattt
     1141 gtctttaagc aaatgatcgt gcaaccattg cgggccggag gtgtaattga gttggttgtc
     1201 caacacatat tccacgcacg gattaccacg gctcatcgca ttaccaatca ccactaaatc
     1261 aggagccggt tgcagttgag caacatcgta attcgggata atttcaatac cgtgagcagc
     1321 taaaaaggta ctcatcggtg ggtaaacatt agtgtctgag ccggtcactt tatagccaag
     1381 ctcacgggca atcattgcca caccgcccat aaaggtgccg caaatgccta aaatatggat
     1441 atgttttttc atctttttat atttaaaatg ttagaatcat cccctctccc cttgtgggag
     1501 agggatagat tttttcttcg tgcagaagaa aaaatcaggg agaggggtaa gcggtcattt
     1561 ttaggcttaa tattgtaagc tccctctctc tgaaaaatat ttctgaacga aatatttttc
     1621 ggtctcaata ctcgctacgc tcgctcctgt ggagccgttg gcaaagccaa cgttcaaatg
     1681 taaacatttg tcccacaagg ggagaggtgc gctttagatg aggcgtacca tccctcgttc
     1741 acatcaatta atcaatattc ttcaactctt cttccgaaag ctgctcttgt tggctttgct
     1801 caacattgcc atctttcact ttaaattcaa ggttttggaa ataagcttca cggaaggtaa
     1861 cataagggtc ttgagcttgc tctaacaacg catcttgttc taataattta gaacgggtat
     1921 ctaccccttg taccacaaat ttagcgatag accaaggtga cccaacataa gtcagcaccg
     1981 aataaccggt ttccaccgca ctaccaatac cttgacgagg tgtggttgct ccataagccg
     2041 gcatcataat ataggagccg gaaggcacgc cgtgagcgcc taaactctca cctaaaccac
     2101 gttgtccgtt atttaaacgt aacccctcat ctaaactcgc ccagtcaatt aacccaccta
     2161 aaccaaacac agtgttgatc cagaagcggt tgaagtgaac catcgctttt ttaccttctc
     2221 cttcaattaa gcggtttaca aagctcaccg gctcatcaag gttattagca acattaatta
     2281 agcctgtttt aaccgggctt ggtacatact ctttccaacc tttagctacc ggttttaaca
     2341 cataagggtc taaatagtcg taattgactt tccacatcgc acggttaaac ccctcaagtg
     2401 ggtcgtctcg cacgccggtt tctgggttaa tgcttgatga acaagcggtt aaaaatgcca
     2461 cactgagtag cataaacatc ggtttgaacg acttgagttt cataaattga accttttgca
     2521 gagaaatttg tccattgtag cgaagttgat aaaaaataca aaacaaaaag ccgttttgcc
     2581 tctagaaatc tagacgtcta gattgagata tagttcgctt ttgattacaa tacaggcaac
     2641 atttaaggag caaacaaatg gcagactgga acggcgaata tattagccct tatgccgaac
     2701 acggcaaaaa aagtgaacaa gtcaaaaaag tgacggtatc cattccaatt aaagtattgg
     2761 aaattttaac taatgagcga acccgtcgcc aaatcaaaaa tcttcgtcac gcaaccaaca
     2821 gcgaattatt gtgcgaggct tttttgcacg cctttaccgg tcagcctttg ccgaatgatg
     2881 aagacttgat gaaagacagg gcagatgaaa tccctgaagc ggcaaaacaa aaaatgtgtg
     2941 agctgggaat agacccggac gaatggcaat attagacaca ccttgcaact gcgaggttct
     3001 ttttatgttt taaggagact ttatgtatca acaattaaca acagaagcac gttgggctgt
     3061 gttgcttacg ctgatttatc tgctcggctg ggtaggtttt gcttactttt caccgcaagg
     3121 cagaggcatt ttcggctttc cgatttggtt tgaactttct tgcattttct tacccgtact
     3181 atttagctta gttgccacca ttgtcgtcaa aaaagtgtat aaaaatatcg atcttgaggt
     3241 gaataatgaa tagtgaaatg attttacccc tgattgctta tttaattttt gtgtttggcg
     3301 tagcgtttta tgcttaccgt aaacgccaag gcggaagctt tatttcggaa tactatatcg
     3361 gtgggcgttc aatgtcgggc tttgtgttag caatgacaac cgccgccact tatgtggggg
     3421 caagttcctt tatcggtggg ccgggtgctg cctataaata cggtttaggc tgggtgttac
     3481 tggcaatgat tcaagtgccc gcagtgctac tctctcttgg agcattaggc aaaaaatttg
     3541 ccttacttgc ccgcaagcat aatagtgtca ccattaacga tatgttatta gcccgttacc
     3601 aaaacaaatt tgtggtttgg attgcaggac tagccttatt actttcgttt tttgcgatga
     3661 tgacggtgca atttatcggt gccggtcgct tacttgaaac cacactcgga atcccttacc
     3721 aaacagcggt aatgattttt gccatcacag tcgggcttta tacctttatc ggcggtttta
     3781 gagcggtggt gctaaccgat actattcaag gtttagtaat gatagtcggc acaagcattt
     3841 tactcgctgg tgtgatttat gccacaggcg gagtggaaaa tgcaatgcac acccttgaag
     3901 ccattaaccc acaacttacc accccttacg gcattgacga acgcccgctt gatttcacct
     3961 ttatgacttc attttgggta ttggtttgct ttggtttaat cggcttaccg catattgcgg
     4021 tcagaagtat ggcatgcaaa aacagccaag ccttgcaccg agcaatgatt atcggcacgg
     4081 tagtcattgc aattctaatg tttggaatgc acctttcagg cgtattaggc agagcggttg
     4141 tgcctgatct caaagtgccg gatcaagtca ttccaacact aatggtacaa gtgctaccac
     4201 cggtggttgc cggtattttc cttgccgccc caatggcagc gattatgtct tcgattgact
     4261 caatgttgat tcagtcgtct tcaaccttaa ttaaagatct ctatctggca attaagccga
     4321 atgccattaa taacgagaaa aaaatcaaat tgttctccac tattactact cttactttta
     4381 cggtattact cgtgtttgcc gccctaaccc cgcctgagat gttaatttgg ctgaacctac
     4441 tctctttagg tggtttagaa gcgactttcc tatgggtaat tgtactaggg ttgtattggg
     4501 aaaaggcaaa tgccaccggt gctatttact caatgctggc aggcttaagt tgttatgtat
     4561 taatgactac ctttaagatt gctattttta gcttccacgc cattgtgcct tcactgctaa
     4621 ttggtttgat tgctttctta atcggcaacc gatttgctcc taaggtgtaa aataaaatag
     4681 gcatagtggt atggaagttc actatgccta ttttgcaaaa tttccgctca attcaaccgc
     4741 ttgtagttaa taagtggcat tattttctgg attattctgc ttagtcgcca ttctaagtaa
     4801 cgtataacct aaaatcgctg aaaccgcaga gccgagtaaa atacctaatc gagctagagc
     4861 ggtaatactt tccccagcct cacttacatc aaaggcaaga cttgctaaga acatcgacat
     4921 tgtaaaaccg ataccgcata gcaccgcaat ggcaaaaatt tgcttaaaat taaccccttg
     4981 cggcaggctg gcaaggcgga ataatactgc taaataacta aaaccgaata cacctaaggt
     5041 tttgccgata attaagccga gcgcaatacc taacggcaat ggtgatgaga gttgctccat
     5101 tcccatttct aataagtaaa cgccggcatt agcgaaggcg aagatcggta aaatcatata
     5161 agcacaccaa ggggcaagaa tatgttctag ctcctctgcc ggtcgttccc catgtttacc
     5221 tttcagcggt acgcagaagc cgatgatcac gccggcaagg gtagcgtgaa cccctgattt
     5281 taaaacggaa gtccatagca ctaagcctac taccaaataa gcacagagtg aggtcacttt
     5341 aaagcggtta agtagcacta agccgataat cgcaatcgcg gcaatagtta aggcttgcac
     5401 actgatttct tgcgaataga aaatggcgat taccacaatc gctcctagat catcaataat
     5461 cgctaatgcc aacaaaaatg cctttaaagc caatggcaca cgagtgccta ataacgccac
     5521 aatgcccacc gcaaaggcaa tatcagttgc cattggaatt gcccaaccat tatgatattc
     5581 agggtggttt tgattaatcg cccaataaat aatcgctgga acaatcatac cacctactgc
     5641 tgctatcgca ggaaaaatag ctttcttata acttgaaagc gaaccttcaa acaactcttt
     5701 ttttacttcc agccccacca acgtaaaaaa cactgccatt aagccatcgt taacccagag
     5761 taatagcggt ttattcaagg caaattcacc aaactgtaca ctaacaatcg tttgtaaaaa
     5821 ctcaaaatag aaatcactta aggaggtgtt ggcgcacaca attgctgcaa aagcaaaaac
     5881 cagaagcaat acgcctcctg aggcttcaga ttttaaaaat tcttttaatt tttccactta
     5941 actttctcct attaatattt gtttattgta ttttcatttc tatacattcc atcttttacc
     6001 aaaaattttg cgaagatcaa atagaaaatt tgataaacta g
//
LOCUS       DANLXQ010000147         6029 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.147, whole genome shotgun sequence.
ACCESSION   DANLXQ010000147 DANLXQ010000000
VERSION     DANLXQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.147"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            24..461
                     /locus_tag="PXD94_002164"
     CDS             24..461
                     /locus_tag="PXD94_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL6286985.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
     gene            complement(746..1474)
                     /locus_tag="PXD94_002165"
     CDS             complement(746..1474)
                     /locus_tag="PXD94_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6286986.1"
                     /translation="MKSFDKVWFSAKELEELDLPDLPKQATNITRKALKELWIKRDAV
                     GVKGGGNEYHITSLPRKAQEALGFKSGINAIKDSIVREITPEEQNKGSELSGTVLNNI
                     ISMIDSFASIKVSAGFGSFNEGVTKPDGQEPYADSLLHTLGVNAKKCAVFWASGDSMY
                     PTIDNGDQLLVDLSRNEIRGDNNIYIVQNGDSVWVKRVKLLWDGVELISDNREEYELI
                     KITADQAQNLQIIGQVVHIGHSLV"
     gene            1684..1911
                     /locus_tag="PXD94_002166"
     CDS             1684..1911
                     /locus_tag="PXD94_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6286987.1"
                     /translation="MNKKKRAQDMHRADIRAELMKKGITFSQLGIENGLSKTTVRNAL
                     DKPYKNGEEIIAKALGKTPAEIWPSRYPDVA"
     gene            1926..3863
                     /locus_tag="PXD94_002167"
     CDS             1926..3863
                     /locus_tag="PXD94_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6286988.1"
                     /translation="MKDWFSAKELAGISGLSKYPTNVTRQAKKEQWATRPLKGVKGGG
                     FEYHINSLPTEVQTEIRSRFMSAVVEAKPKKLPVVRAEVDVGNLTTKQREIADARMAL
                     VAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLAELVAVANAKKTAKRTLSVRTLNGW
                     VVDYCKAENAEQRLKLLAPQVRQETKPEEIWWLSAFLGVYRQKNGICLTEAYREFEVE
                     WAYQYADNPLLLAQCPSLSQVQRAMNKLPLYVKEYGRRTGSHYKQLLSYVKRDWSVLR
                     ANDVWIGDGHSLKLKVAHPIHGKPFTPELTMIVDGAGRKVVGWSLALSENAFAVADAL
                     RHAISLHGVPAIYYSDNGGGEKNKFLDAEITGMLPRLGIRHETGIAGNPQGRGIIERL
                     NKTIGMTIARQFETGYATGADPETVRKTLYAVNSLANAKGELTPLQRKAQGKLPTWQQ
                     LIEVIQSVIDWYNNEHIHSEIRTTPARKYQQMLHAEDVVMLSPIELRDMSRPEFIRKP
                     DRGWVSWNNNHYFNLKLLDFDREEVVIGIDIHNAESVQVRTKDGRFICEAIWNGNTRE
                     AFPVAMVEQQRKERHKRRANLKQQQLDEINAELNPVLTIEQKNDFSLLATARKPKKEV
                     EPIFLTRAEKEDYEKKLAVGG"
     gene            3867..4748
                     /locus_tag="PXD94_002168"
     CDS             3867..4748
                     /locus_tag="PXD94_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6286989.1"
                     /translation="MKNQELRALMDSKGYQQKQVAQLLGVSVATVSLYLKGDYNGNVA
                     EMDRKVDELIERDKAKVVEAKYNAAFVPTLAARRGMEVMQFAHIEGEINVIFGAAGLG
                     KTQMLKEYERRNSSATLIEVDPSCTPKVLLRKIAEAVGANARGVNNELLESIVNKLKG
                     SERLLMIDEAELLSTRSLEFIRRIHDLTSVGVVLAGMPRLLVNLKGKNNELAQLYSRV
                     GFACDLGNALSEEDLGLLAESALGTNEFNAPLIKACHGNARRLSKLMRGVVRSSEINQ
                     TPISKDLVEQYSKMLIS"
     gene            4759..5079
                     /locus_tag="PXD94_002169"
     CDS             4759..5079
                     /locus_tag="PXD94_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286990.1"
                     /translation="MQKVYSEKAGEMMNPLNAFIHDSLVKLEIATLECEAMELEVERV
                     EFEAKSPPRLVLRDNCKTQDLVKNGLASLFGMKTKGGRRYDSYQMQVRGVKCVWESER
                     VTKV"
     gene            5082..5273
                     /locus_tag="PXD94_002170"
     CDS             5082..5273
                     /locus_tag="PXD94_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286991.1"
                     /translation="MKKRRMIYALYVGEENVADGTAEELAERLGVEAKTVRFMSSPAH
                     NKRVKYNGKAIRTVKVGLE"
     gene            5286..5903
                     /locus_tag="PXD94_002171"
     CDS             5286..5903
                     /locus_tag="PXD94_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HDL6286992.1"
                     /translation="MSKVTIGNEIYWKDADGNLKPEALVKEIDKERDELVRQFVSKAQ
                     ALSKALGDFKQSVFDDVGAFVSLSADKYGVKIGGAKGNITLFTYDGEYKLQLAVQENI
                     RFDERIHAAKALIDECLHDWSEGAKPELKALIDNAFEVDKEGNLSTAKILSLRRVEID
                     DARWNQAMTAISDSVQVIGSKGYIRFYKRDENGKYQPISLDMAGV"
BASE COUNT         1888 a         1113 c         1452 g         1576 t
ORIGIN      
        1 aaaaatttga aagagataat cctatgttaa gcttatttga tgcaggacag cgttacctca
       61 acactctgcc gaatcagaaa aaattagcca attttatgcc agattaccac attattcgct
      121 tggtaaaatt ttcctctaaa gtaatgcctg cttttgcttg ctttgcatta gtttggcaat
      181 attgctttac cgatccgacc caatctattt tagccaattc ggtacttact gctgttttcg
      241 ccatttctat tccgtttcaa gggttatatt ggcttggaaa acgggcaaga tcaccgttat
      301 ctttatcatt attggaatgg tatgagtcgc ttagacaaaa attaatttcg gcacaagtca
      361 aattagaaga aacacaagcg gtaccttctt tccaagattt tgcaaattta ctcaagctag
      421 cagaagagac ttggggagat gaatattttg atgagttata aaataaaaaa agccccactt
      481 aaaataagtg gggtaaaact tttgttctaa ttctggaggt ttgaattact gttttattgt
      541 ggggtactga tcttttttgc atagagagaa agaaaaaaat ttgggcggaa aaataacggc
      601 gagccccgtg aatacaaggg ttcgccgtta ttgtttggtt ggagctatgc aaaaaacaaa
      661 atgagcagga ttagaaaaaa acgagcagtt tttgcgttat tttttgcata ggtttgatcg
      721 gttttaaaac tattttaaat ctgttttaaa ctaaagaatg cccgatgtgt accacttgcc
      781 cgatgatttg taggttttgg gcttggtctg ctgtaatctt tattaactcg tactcttctc
      841 ggttgtctga aataagctct acaccgtccc atagcagttt tacacgctta acccaaacac
      901 tgtcgccgtt ttgtacaatg taaatattat tgtcgcctcg tatctcatta cgacttaaat
      961 caactagtag ctggtcgcca ttatcaatag tcggatacat cgagtctcca cttgcccaaa
     1021 aaacagcaca cttcttggca ttaacaccaa gcgtatgcaa caagctatca gcataaggct
     1081 cttgtccatc aggttttgtt acaccttcat taaagctccc aaagcctgca gatactttaa
     1141 tagatgcaaa actatcaatc attgagatta tgttattcaa aacggtgcct gataattcag
     1201 atcctttgtt ttgctcttca ggtgtaatct ctcttactat gctatccttg attgcattta
     1261 ttccactttt aaatccgagt gcttcttggg ctttacgagg caatgaagtt atgtgatatt
     1321 cattcccccc accttttact ccaactgcat cacgttttat ccagagttct tttaatgcct
     1381 ttcttgtaat gtttgttgct tgctttggaa ggtcaggcaa atccaattct tctagttctt
     1441 tggctgaaaa ccaaacttta tcaaatgatt tcataattaa ccccttctta atcaaaaatg
     1501 attttaaaat caaaattgat taagttattt aaaatcaata gcttacaaat agatttaaaa
     1561 aagatttaaa aaatttatta aatcactatt gatttagaaa agaataagta ttatatttag
     1621 tcctgtagtt aatcaagtac gctaaagcgt agcaaataaa ctaaatgtgg aggatctcac
     1681 aaaatgaata aaaagaaaag agctcaagat atgcatagag ctgatattcg agctgaattg
     1741 atgaagaaag gaataacatt ttctcagtta gggattgaga atgggctttc caaaacaaca
     1801 gtaagaaatg ctcttgataa accttataaa aacggtgaag aaattatcgc aaaagcatta
     1861 ggaaaaacgc cagccgaaat atggcctagt cgttaccctg atgttgcata acaaggatag
     1921 ctgttatgaa agattggttt agtgcaaaag aattagcagg tattagtggc ttatcaaaat
     1981 acccaacaaa tgtaactaga caagccaaaa aagaacaatg ggcaactcgt ccattgaaag
     2041 gggttaaagg aggtggtttt gaatatcaca ttaactcttt acccaccgaa gttcaaaccg
     2101 aaatccgctc ccgctttatg tcggcggtgg tggaagccaa gccgaaaaag ttaccggtag
     2161 tgcgtgcaga ggtggatgtg ggaaacctga ccaccaaaca gcgagaaatt gctgatgcac
     2221 gaatggcact ggtggcgtat gtgttggagt tggaaggctc aatgagccgg attaaagcga
     2281 taacctacct ttgcaacctt gccaaacaag gcgaaatgcc accgcacttg gcggagctgg
     2341 ttgccgtagc aaatgctaaa aagacggcaa aacgcacact ctcggtgcga accttaaacg
     2401 gctgggtggt ggattactgc aaggcagaga acgccgagca acggttgaaa ctgcttgccc
     2461 cacaggtacg gcaggagacc aagccggaag aaatttggtg gctaagtgct tttttaggcg
     2521 tatatcggca gaaaaacggt atttgcctca ccgaagccta ccgagaattt gaggtggaat
     2581 gggcgtatca gtatgcagac aacccgctac tgttggcaca atgccccagc ctaagccaag
     2641 tacagcgagc gatgaataag ttgccgttgt atgtgaaaga gtacggcaga cgcaccggct
     2701 cgcattacaa gcagttactg agctatgtga agcgggattg gtcggtactt cgagcaaatg
     2761 atgtatggat tggtgacggt cactcactga agctcaaagt agcccacccg atacacggta
     2821 agccgtttac ccctgagctg acaatgattg tcgatggtgc tggtagaaaa gtggtgggct
     2881 ggtcgctggc actcagtgag aacgcctttg cggtggctga tgcactacgc cacgccatta
     2941 gcctacacgg tgtgccggca atctactact cggataacgg tggtggtgaa aagaataaat
     3001 ttttggatgc ggaaatcacg gggatgttgc cccgcctcgg tatccgacac gaaacaggga
     3061 ttgcgggcaa tccgcagggg cgaggcatta ttgaacgctt aaataaaacc atcggtatga
     3121 cgattgcccg acagtttgaa accggctatg caaccggtgc agatccggaa accgttcgca
     3181 aaaccctgta tgcggtgaac tcactggcaa atgccaaagg ggaattaacc ccactacaac
     3241 gtaaagccca aggcaaattg ccaacgtggc aacagttgat tgaagtaatc caatcggtga
     3301 ttgactggta caacaacgag catattcact cggaaatccg taccacgccg gcacgcaaat
     3361 atcagcagat gttgcacgca gaagatgtgg tgatgttaag ccctattgag ttgcgggata
     3421 tgagccgacc ggaatttatc cgtaagccgg atcgaggttg ggtaagttgg aataacaacc
     3481 actattttaa cttgaaatta ctggattttg accgtgaaga agtggtgatc gggattgata
     3541 ttcacaatgc cgagagcgtg caggtgcgta ccaaagacgg caggtttatt tgcgaggcaa
     3601 tttggaacgg taacacccga gaggcattcc cggtggcgat ggtagaacag cagcgtaaag
     3661 aacgccataa acgccgagcc aaccttaaac agcaacagct tgatgagatt aatgcggaat
     3721 taaacccggt tttaaccatt gagcagaaaa acgattttag cttgctggca acggcaagaa
     3781 aaccgaaaaa agaagtagaa ccgatttttt tgacccgagc agaaaaagag gattacgaaa
     3841 aaaagttagc agtgggaggc taaacgatga aaaaccaaga acttagagcc ttaatggata
     3901 gcaaaggcta tcaacaaaaa caagtggcac agcttttagg tgtatccgtt gccacagtca
     3961 gtctgtatct caaaggcgat tataacggca atgtggctga aatggatcgc aaagtagacg
     4021 agctgattga gcgtgataag gcaaaagtgg tggaagcgaa atataacgct gcttttgtgc
     4081 caactttggc ggcacgcaga ggaatggagg taatgcagtt tgcccatatt gaaggcgaaa
     4141 ttaatgtgat ttttggggca gccggcttag gtaaaaccca aatgctcaaa gaatacgaac
     4201 gcagaaacag ctctgcaaca ttaattgagg ttgatccgag ttgcacacct aaagtcttgc
     4261 tccgcaagat tgccgaggcg gtgggggcaa atgccagagg ggtaaataac gaactattag
     4321 aaagcattgt gaacaagtta aaaggctctg aaaggttgct aatgattgat gaagctgaat
     4381 tactttcgac ccgctcgctg gagtttatcc gccgaatcca cgatttaacc agcgtgggcg
     4441 tggtgttagc cggtatgccg agattgttgg ttaatcttaa aggtaaaaat aatgagctgg
     4501 cacagcttta tagccgagta ggttttgctt gtgatttggg aaatgccttg agcgaggaag
     4561 atttggggct attagccgaa agtgcgttag gtacaaatga atttaatgcc ccgctaatta
     4621 aagcttgtca cggcaatgcc cgccgattaa gtaagttaat gcgtggcgtg gtgcggtcta
     4681 gcgagattaa ccaaacacca attagtaaag atttggttga gcaatatagc aagatgttga
     4741 ttagctaagg agcaagttat gcaaaaggtt tacagcgaaa aagcaggcga aatgatgaac
     4801 cccttaaatg cgtttatcca cgacagcctt gtgaagttag aaattgccac tttggaatgt
     4861 gaggcgatgg agttggaagt tgaacgagtg gagtttgaag cgaaaagccc accccgcttg
     4921 gtcttacggg ataactgcaa aacacaggat ttagtcaaga acggcttggc aagcctgttt
     4981 ggaatgaaaa ccaaaggcgg cagacggtat gacagctatc aaatgcaggt gcggggggtg
     5041 aaatgcgtgt gggagtctga acgtgttacg aaggtgtaga aatgaaaaaa cggcgaatga
     5101 tttatgccct gtatgttggc gaagagaatg tggctgacgg tacggcggaa gagttagcag
     5161 aacggttggg ggtggaggcg aaaacagtgc ggtttatgtc gtcccctgct cacaacaaaa
     5221 gagtgaaata taacggtaaa gcaatccgca ccgtgaaagt cgggttggaa tagagaggaa
     5281 gtattatgag taaagtaaca atcggtaatg aaatttactg gaaagatgct gatggcaatt
     5341 taaagccgga ggcattagta aaagaaatcg acaaagaacg tgatgaatta gttcgccaat
     5401 ttgtaagcaa agcacaagcg ttaagcaaag cgttaggtga ttttaaacaa tccgtatttg
     5461 atgacgtggg ggcttttgtc agcctatcgg ctgacaaata tggagtaaaa attggcggtg
     5521 caaaaggcaa tatcacacta tttacttatg acggtgaata taagttgcag ttagcagtgc
     5581 aagagaatat tcgctttgat gagcgtattc acgcagcaaa agccttgatt gatgaatgct
     5641 tgcacgattg gtcggaaggg gcaaaaccgg agcttaaggc cttaattgat aatgcgtttg
     5701 aggtggataa ggaaggtaat ttatctacag caaaaattct ctctctccgc cgtgttgaga
     5761 ttgatgatgc aagatggaat caagcaatga cagcgatttc agatagcgta caagtgatcg
     5821 gtagtaaagg ttatatccgt ttttataaac gtgatgaaaa cggaaagtat cagccgatta
     5881 gtttagatat ggcgggggtt tagaatgttt attactttta atgttaatta tacggataaa
     5941 cctgttgtgg ttaatacaga caaggtttgt tcaatagaaa atattaacgg gaatgtaaca
     6001 gtacactttt gtgataacaa aaaattaat
//
LOCUS       DANLXQ010000148         5982 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.148, whole genome shotgun sequence.
ACCESSION   DANLXQ010000148 DANLXQ010000000
VERSION     DANLXQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.148"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(3..1409)
                     /locus_tag="PXD94_002172"
     CDS             complement(3..1409)
                     /locus_tag="PXD94_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL6286993.1"
                     /translation="MKLTKIVLSVVAVIGAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFISNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMGEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNPQTLENEKTGELLLQLLAKSFKFHINNFSLE
                     NSKGKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAEDIIRQVSIVTE
                     KLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGKVNLNGRELTED
                     ELQMALFMIVMGAGSLGQ"
     gene            complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXD94_002173"
     CDS             complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXD94_002173"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL6286994.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPTAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQAGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLADQANLLKELAEFVQVMKAATK"
     gene            complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXD94_002174"
     CDS             complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXD94_002174"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL6286995.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEEIKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            complement(3469..4221)
                     /locus_tag="PXD94_002175"
     CDS             complement(3469..4221)
                     /locus_tag="PXD94_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL6286996.1"
                     /translation="MKIALVTGVTAGFGLAICKLLLQSGYKVIGTGRRAERLEELHLQ
                     FGDKFLPLAFDIQQPDEVEKALNSRPKAWQQIDLLVNNAGLALGLEPAQKADLNDWYQ
                     MIDTNIKGLVTMTRLVLPEMVERKSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSL
                     NLRADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVL
                     WLNQQPEHVNINRVEVMPVAQTSSALNVARNI"
     gene            complement(4447..5979)
                     /locus_tag="PXD94_002176"
     CDS             complement(4447..5979)
                     /locus_tag="PXD94_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL6286997.1"
                     /translation="MNLVDYSTSLWSVLPALIALILAIVTRKVIISLSIGILFGAFML
                     TNSNFLDALTYIKNSVVSLVYKGAEEGLNSNNVNIILFLLLLGVLTSLLSISGSNQAF
                     ANWAQKKVKGKRGAKLMAAGLVFFTFIDDYFHSLAVGAIARPVTDKFRVSRAKLAYIL
                     DSTAAPMCVLMPISSWGAYIITLIAGLLATHSITGYSPLGAFMTMSAMNFYAIFAMIM
                     VFFVAYFSFDIGSMARFERQAAHLAELDDEQIETKGHVRDLILPIIALIVATVSMMMK
                     TGADALAESGASFSILGAFENTTVGISLVVGGVAGLIVATICIITSGNISFKNYVRAY
                     GLGVKMMSGAITILFFAWTINGVVSDMQTGKYLSGLVAESIGAEFLPAILFVLAAAMA
                     FSTGTSWGTFGIMLPIGASMAVHSDPALIIPCLSAVMAGAVCGDHCSPVSDTTILSST
                     GAQCNHMDHVISQLPYALLVATASIIGYLVVGFTQSAVLGFIATAVVMAILILLFKEK
                     KH"
BASE COUNT         1793 a         1358 c         1040 g         1791 t
ORIGIN      
        1 gattattgcc cgagtgagcc tgctcccatc acaatcataa acagagccat ttgaagttca
       61 tcttcggtca gttcacgtcc gtttaaattc acttttccgt tatctatagt taattcaatc
      121 ttcacattgt tgtcatctac ttttcttaat ccgtactgtt caactcccgc attgagaaaa
      181 acagcatcaa cttgttgctt ggcaaatgct ttcgcctctt cttcgcctaa tttttctgta
      241 actattgaga cttggcgaat aatatcttcc gcatattgac ggttgatatt tgaagtgaat
      301 ttacttgttg aaagggcttg caataccgct tgcatattac caagattttg tgggtcaaat
      361 tgagccatat tcagtaataa agccaaatca actttgcctt tgctgttttc aagtgagaaa
      421 ttattaatat ggaatttaaa tgattttgct aatagttgta ataataactc gcctgttttt
      481 tcattttcta atgtttgagg attagataat aaaggcgtaa tatcattggt taatttagca
      541 tctaaatcgt atgccatatc catttttaat ttacccattt caacaccctc aatgttgaaa
      601 gtggctgcct ctaaatctcc ggtagaaatt aaacgatcgc cttttaatag attatcgccc
      661 ttcaccacaa catctttaat ttgagataat ttgccatctt tagacttaaa ctcaatagcg
      721 ttaatttttc cactaccttt gcctaaagtt aaattaggat aactttcatt attttctgtt
      781 tgaacatcat aaactatacc ttgaatttga aaatcaaatt cttctgcttt tatcttaaga
      841 ctttctagct caacagtgct atcaacccct tttaattttt ggtcgtagct atattgcact
      901 ttgattggcg tggtttcaat atgacctgtt tcatcactat gtttaatcgg ggaaatatca
      961 aaatgccctt ccacatcgcc cgaatagctg atattagaaa aaccggtgcc aatatgttca
     1021 cccatcaatt ttttaagggc ttcaggagct tgtaatttgc tttccgcact catcattaca
     1081 ggagctaaat taagtttcgc taaacgatta agcggtagcg gaccgtgatg aagtttatca
     1141 ttaccaacaa agtccaattt cttaccatta cccaaatcca cttctaaacg atatttcgca
     1201 ttagagataa agaaatggcg atcgatctgc acatctttaa gctcaatatt cgcaccaaat
     1261 tcgccactca gttgtttaag ttggttatta gcttgaacaa ccaattcttg atatttttct
     1321 tcaacctgct tacctgtgta ccaacttccg ccaactgcaa cagcaccaat taccgctaca
     1381 actgaaagaa caatttttgt taatttcatt ctgtttcctt ttattttcaa aaaaatttaa
     1441 aaaatgaccg cttgtagttt acttcgtagc cgctttcatc acttgtacaa attcggcaag
     1501 ttctttgagc aaattagctt ggtcagctaa attacgttca ataattttca caatcgctga
     1561 gcctgaaatc gccccgtctg ccccaagcct taacgcctct tttacctgtt caggtttggc
     1621 tatgccaaaa ccttgtaaga taggggcagc tccggcttgt tttaaacttt ccactaaatt
     1681 atccaaatta cttgcgtgag cttggttttc agcactagtt acaccggcac gggaaactaa
     1741 ataagtgtag ccttgggtta attgagcaat ccgttcaatg gtgttttgat cagcattcgg
     1801 cgggcaaata aacaccggct gaattccata tttttcagcg gttggcacaa actcttgagc
     1861 agcgaaaaca ggcaaatcag caatcaacac cgcatcaact ccactttgtt ggcaacgttg
     1921 gaaaaatact tctgcggttg gcacaaaaat taaattggca cacagtagta aaccgatagg
     1981 aatgtctgga tatttagctc gaattttggc aatcagctca aaacagctat cagtgctata
     2041 gccaccatct aaagctcgtt tattcgctgc ttgaatcacc ggcccgtcta agagggggtc
     2101 agaaaacgga aagcctagct ctaaagcatc agccccgttt ttaatcatcg tgtcgataat
     2161 ctcaaaactg cgttcaaaac caggatcgca taatgtcaca aacggcacaa aagccccttc
     2221 gttttttgct tttaatgcag taaatagcgt atcaaagcgg ctcattttac tctcctccat
     2281 ttaaaatttt atccacggtg aaaatgtctt tatcgcctcg acccgataaa ttcaccacta
     2341 ataactgctc tttttcaggg ttctgcttaa tcatttttaa cgcataggct aaggcgtggg
     2401 aagattctaa cgccggaata atcccctcat ttttggctaa tgcttggaat gcctctaaag
     2461 cctctgcatc ggtaatcgat acatattcag ctcgccctgt cgcatttaaa taagcgtgtt
     2521 gcggcccgac agaagggaaa tctaagccgg ctgaaatgga gtaagactct tcaatttgcc
     2581 cgtctttatc ctgcataatc ggcgatttca taccgaaata gatccccgtt gtgccgtgtt
     2641 tgagcggtgc accgtgttca ccggtttcaa ttccttttcc tgccggctca acaccgatca
     2701 atttgacgga ttgctcttcg ataaaatctg caaacatacc gatagcattt gagccaccgc
     2761 ctacggcagc aatcaccgca tcaggcaagc ggtcttcttt tgctaaaatt tgacgtttga
     2821 tctcttcgcc aatcattttc tgaaattcac gcaccattgt cgggaacggg tgcgggccgg
     2881 ctgcagtgcc gattaggtaa tgggtagtat cataatttgc cgcccaatcc cgcattgcct
     2941 cgcaacaggc atctttgagc gagcaagagc ctttttgtac cggaatcacc tccgctccca
     3001 ttaaacgcat acggaataca tttggcgatt ggcgttctac atcttttgcg cccatataca
     3061 ccacgcaagg cattcctaac atcgcacacg ctaatgcggt tgccacaccg tgctgtcctg
     3121 ctcctgtttc ggcaataatg cgggttttac ccattcgttt agcgagtaag atttgcccta
     3181 gcacttggtt ggttttatgt gccccaccgt gtaaaagatc ttcacgtttt aaatagagtt
     3241 tggttttact gcctttggtt aaattacgac aaagggttaa agcagtcggg cgaccggcat
     3301 agtttttgag taaatcggca aattcagctt ggaatgttgg gtcttgctga gcatctacaa
     3361 aggcttgttc taattctttt agcaccggca ctaaaatttc cggcacatac ataccaccaa
     3421 attcaccaaa ataaggatta agaagtgtgt ctgacataat atttcctttt aaatatttct
     3481 cgctacattt aaggcagaag aagtttgagc caccggcatc acttcaaccc gattaatatt
     3541 aacgtgttca ggttgctgat tcagccaaag cacaatattt gccacatctt ccgggcgcac
     3601 aaactgcacg ttttcataaa ctttagccgc tttttcatca tcgcctttaa agcgtacatt
     3661 tgaaaattcc gtaccgccac atagccccgg ctcaacattg gaaacacgca ctttagtgcc
     3721 ggctaaatct gcccgcaaat ttaggctaaa ctgtttcacg aaggctttag taccaccata
     3781 tacattaccg cccgggtaag ggtaagtgcc ggcaatcgag ccgatattaa taatatgccc
     3841 acttttccgc tccaccattt caggcaaaac taagcgtgtc atcgttacca atcctttaat
     3901 attggtatcg atcatttgat accaatcgtt caaatctgct ttctgtgccg gttctaagcc
     3961 gagtgcaagg cctgcattgt ttactaataa atcaatttgt tgccacgctt tagggcggct
     4021 attcagtgct ttttccacct catcgggctg ttgaatatca aatgcaagcg gtagaaattt
     4081 atcgccaaat tgtaaatgca gttcttctaa acgctctgcc cgtctgcccg tgccaattac
     4141 tttataaccc gattgaagca ataatttaca aattgccaac ccaaatccag cagttactcc
     4201 tgtgactaat gctattttca tcttttcacc ctcaaattta aaatgtacta ttaaagtagt
     4261 acaaaaaaat taaaattgca agaaataata ttgccataaa agaaaaaagc gtaccaaaag
     4321 gtacgctaat gctaggagga tcttatttta agatttacat aacacaacat tcgatatcca
     4381 tatcttgagt gctattttat ctcatcaata aatctcattc tgtgattccc ttcacatttt
     4441 cggaaattaa tgcttttttt ctttaaaaag caaaatgagt attgccataa caacagctgt
     4501 agcaataaat cccaataccg ctgattgggt aaagccaacc actaagtaac caataataga
     4561 agctgttgca actaataaag cataaggtaa ttgagaaatg acatgatcca tatgattaca
     4621 ttgtgcgcct gttgaagaaa gaatagtggt atcagatact ggagaacagt gatcaccaca
     4681 aaccgcacca gccattactg cagataaaca aggaataatt aatgctggat ctgaatgcac
     4741 tgccattgag gctccaattg gcaacataat accaaatgtt ccccagctag ttcctgttga
     4801 aaacgccatt gctgcggcta acacaaatag aattgcaggt aaaaactctg ctccaatgct
     4861 ttctgcaacc aatcctgata gatatttccc tgtttgcata tcacttacta cgccgtttat
     4921 tgtccacgca aagaagagaa tagtgattgc accagacatc atcttcacac ctaagccata
     4981 agcacggacg taatttttaa aagaaatatt accgcttgta atgatacaaa tagttgcaac
     5041 tataagccct gccacaccac caacaactaa tgaaatgcct accgtagtat tttcaaaagc
     5101 acctagtata gaaaatgaag cacctgattc ggccagtgca tctgcacctg ttttcatcat
     5161 cattgagact gtagcaacaa taagtgcaat aattggcaaa atcaaatcgc gcacgtgtcc
     5221 tttcgtttca atttgctcat catctaattc tgctaaatgc gctgcttgac gttcaaaacg
     5281 cgccatcgaa ccaatatcaa atgagaaata tgccacaaag aacaccataa tcatcgcaaa
     5341 aatggcgtaa aagttcattg cgctcattgt cataaatgcg cctagtggcg aatagcctgt
     5401 gatggaatgc gtagccaata agccagctat taaggtaata atataagccc cccagcttga
     5461 aattggcatt aacacgcaca ttggagcggc ggtagaatct aaaatataag ctaatttagc
     5521 acgagaaaca cggaatttat ccgtaacagg gcgggcaatc gcacctactg ctaaactgtg
     5581 gaaatagtca tcaataaagg taaagaaaac caaacctgcc gccattaatt ttgcgcctcg
     5641 tttgcctttt acctttttct gcgcccaatt cgcaaacgcc tgattactac ctgaaatact
     5701 taataatgac gttaataccc ctaataaaag caaaaataaa ataatgttaa cattatttga
     5761 atttaatcct tcttcagccc ctttataaac taaagaaaca acgctatttt taatataagt
     5821 aagtgcatca agaaaattgc tattagtaag cataaatgca ccaaagagaa taccgatgct
     5881 aagtgaaata atcaccttgc gtgtcacaat tgccaatatt agcgcaatca gtgccggcaa
     5941 taccgaccaa agtgacgtag aataatcaac tagattcata aa
//
LOCUS       DANLXQ010000149         5800 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.149, whole genome shotgun sequence.
ACCESSION   DANLXQ010000149 DANLXQ010000000
VERSION     DANLXQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.149"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            573..842
                     /locus_tag="PXD94_002177"
     CDS             573..842
                     /locus_tag="PXD94_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6286998.1"
                     /translation="MKNKNFEIAKVVILNEQLGSPKDLELKVVENSLSDEDRYDIKQA
                     VLKAVGNGCSDPEMLASRCCSAIEIINRNGLNTGNGSISTSSNSV"
     gene            complement(820..1092)
                     /locus_tag="PXD94_002178"
     CDS             complement(820..1092)
                     /locus_tag="PXD94_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas2"
                     /protein_id="HDL6286999.1"
                     /translation="MRNYLISYDLYKAGQNYDGLISYIKSHQVWAAIHKSVWYIKSNK
                     SAEQIRNELLIYLDKNDSVFVTDMNSAAWNNLTEGNGEYIRQNWMR"
     gene            1373..1448
                     /locus_tag="PXD94_002179"
     tRNA            1373..1448
                     /locus_tag="PXD94_002179"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1406..1408,aa:Arg,seq:tct)
     gene            1760..2266
                     /locus_tag="PXD94_002180"
     CDS             1760..2266
                     /locus_tag="PXD94_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287000.1"
                     /translation="MEAKYNMIIELAQQQGLTHIQLTQKHCLHLAASVGKLCSGVVNN
                     AKQKIKTATGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELYGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            2270..2629
                     /locus_tag="PXD94_002181"
     CDS             2270..2629
                     /locus_tag="PXD94_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287001.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            2626..3105
                     /locus_tag="PXD94_002182"
     CDS             2626..3105
                     /locus_tag="PXD94_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287002.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKALNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFTPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNALAEMVKRTQEANNPKHA"
     gene            3239..3451
                     /locus_tag="PXD94_002183"
     CDS             3239..3451
                     /locus_tag="PXD94_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287003.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFLLVEEKIIDVLENNGFETVAT
                     ILAGSDGWCNAINAYLAEQNKHRNEF"
     gene            3464..4387
                     /locus_tag="PXD94_002184"
     CDS             3464..4387
                     /locus_tag="PXD94_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL6287004.1"
                     /translation="MTNQNQQVAQKQDKFPIKTLFESPNVKQKLQELLNKNAATFTTS
                     VLQIVNSNSMLRNAEPMSVFNAACMAATLNLALQNGLGFAYIVPYQNRKEKKTEAQFQ
                     LGYKGLIQLAQRSGQFKRLVAVPVYEKQLLAEDPINGYEFDWKQKPAKDEKPIGYYAY
                     FKLINEFTAEIYMTTQEVHDHANRYSQTYRKGYGVWHDNFEAMALKTVMKLLLSKQAP
                     LSVEMQQAVLADQAVVKDVENAEFAYPDNEIQEAEFTELKVSDEVFEKCKQTIINKES
                     TLQDMCDNGYEFNKEQYAELEKLENEQQSNV"
     gene            4380..5042
                     /locus_tag="PXD94_002185"
     CDS             4380..5042
                     /locus_tag="PXD94_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB precursor"
                     /protein_id="HDL6287005.1"
                     /translation="MYKFKPRCSMLHQLMSEPRTKADREAGLISDTAKAAVREVAKFD
                     LFGYQSFDGNKYTEKGNQLEEQAIKLSGFTRGLVLKKNTERRENDWISGECDIYVPSR
                     KLIIDTKCSWDIGTHPFFADEAIAKAEKAGYDIQMQGYMWLWDCEEAQIDFVLFPTPI
                     DLIGTYESTEKLIDLVEQIPQQKRITTVTVKRDEAIIAKIKDRVKAASKYYDKLIQEA
                     MN"
     gene            5083..5784
                     /locus_tag="PXD94_002186"
     CDS             5083..5784
                     /locus_tag="PXD94_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDL6287006.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAITTFGTVFAGTLGGYIE
                     KESNLKHLGNAWVYDNAKVYDSAEVYGNAKVYDSAEVYGNAEVWGSAEVYGNAKVWGN
                     AEVWGSAEVYGNAEVWGSAKVWGNAEVWGSAEVYGNAKVYDSAEVWGSAKVRSFAVLS
                     ERKMIFWASNVGTENGTLTVYNGKDGLLVTRGCFTGTVEEFLAKSDEVHDEKTKREYQ
                     LLIEVAKSRILGEAT"
BASE COUNT         1999 a         1197 c         1206 g         1398 t
ORIGIN      
        1 ccttgaataa accgccagga gggcggtttt ctcttgccta aaatctgcaa aattctcttc
       61 aaaaacgacc gcttgcaact caatctgttt atttattaag caatcaaaca acctttctaa
      121 aaataaattc cttttaaaat caacaaatta cctttttgtt atttattttt accttattta
      181 acataccttt tggtatttac aacaatattt accataaggt ataatgcacc catcaaaacg
      241 agctacggct caatgctctt taaaaatcaa attcaaaacc gctttaagcc taatggtgaa
      301 aagtaggaag acgaaagtcg gaggcagccc ttaaaacgcc cactacttca aagcgttatg
      361 ccctttggtg caagcaaagg aatacaagca tataccctta tagcggttta ggtgaaagtc
      421 tgggcgtgta cattgagaca caagggagta accgaaattc aaattgtgca aacgcagctc
      481 taaaattgcg tgacagctcg gagagacggc atttcaaatt ccaaagcata tttgagaaag
      541 tgtgttttgg aatatttaac tagaggtaaa ttatgaaaaa caaaaatttt gaaattgcga
      601 aagttgttat tttaaatgaa caactaggaa gccctaaaga tttggaatta aaagtagtag
      661 agaatagcct atctgatgaa gatagatacg acatcaaaca ggcggtactt aaggctgtag
      721 ggaatggttg ttcagatcca gaaatgcttg catcacgatg ttgttccgca atcgaaataa
      781 ttaatagaaa tgggctaaac actggtaatg ggagtatatc tacctcatcc aattctgtct
      841 aatgtattca ccatttcctt cagtcagatt attccaagca gcactattca tatccgtaac
      901 aaagacacta tcgttcttat ctaaataaat gagcagttca ttacgaattt gttctgcaga
      961 tttattagat ttgatatacc aaactgattt atgaatagca gcccaaactt gatggctttt
     1021 aatataacta atcaatccat cgtaattttg cccagctttg tataaatcgt aagaaattaa
     1081 atagtttctc atagaagaat ccttatttgt gctgtgagag atttaattat attccttagt
     1141 gttgtgagag acaataagga aacttgacgg ctcacaagta taaaagagcc accatctcaa
     1201 accgctctca acaaagagtt agcccgataa cctcacttgg ttttttgcgt atgttttccc
     1261 aaagtgccgt tatccagcga aacgaatgga ctagagagcg gtttgagatg gtgaaagcca
     1321 tcctccttag attgaagtaa accctgccgt tggggttaag caacggtact ttgaccgcat
     1381 agttcagtgg atagagcagc tgccttctaa gcagtgggtc gagagttcga atctctctgc
     1441 ggtcgccatc caaaaccgct ttgcgcacat catcgatgac gagcctacga agtggaaacc
     1501 taccgtgtag agcggttttg aatggctaat aaaacaatgt gaggtatcaa atgcttatta
     1561 agttaaaaag ccctgaacaa gcagggcaag aagaaatctg ggaagttagc gatgattgga
     1621 ttgctcctca agaattaaat ccgccatcag aaacatctcc ttaatataac gcctgtagag
     1681 ttgctcaagg ttaagtaatt actgcggagg ggctttttac acccaaaatt cagatttttg
     1741 actaaaaaag gaacgaaaaa tggaagctaa atacaacatg attattgagc tggcacaaca
     1801 acaaggctta acccatattc aacttactca aaaacactgt cttcatcttg ctgcatcagt
     1861 ggggaaacta tgttctggag tcgttaataa tgcgaaacag aagattaaaa cggctacggg
     1921 agaaattttt acattactca ccatcattaa ccatcaaatc cagttcgatg aagacttaga
     1981 gcttcacacg tggaaagcta gtggaacaac cccacccgag caaagagcga tacgtatgtt
     2041 acatcatacc tcgcaatttg cttggtttgc tccttggtta gtggacagca atgaaaaaga
     2101 acaaaaccaa aatacaatca ggattaattt atttaacaaa tgctctggga ttctgattga
     2161 actatatggc attcttaaag aatacgagct aactcttgat gagtgcttag atgaattctt
     2221 gaaaaataag cattgtttga atgaatttag tattcacaag gagtaaaaaa tgacgacgtt
     2281 tagtatttat accagtgtat ggcacgacag ctccacgaaa ggctttcgcc acaacattaa
     2341 gcataaacgc cacgattgct ggagagcgga tatccgcata aagcaaaaaa tcaatgatgt
     2401 gtgggtgcag atttcaagaa tacgcaaacg cttcaagtgc cctgaacagg caaaatcctt
     2461 tgtagaacaa tggaaacaaa ttgattcccc tgcatttatg caacaccaaa tcaaccgcca
     2521 agcattaaga gaagaattat caaaccttga gcaacgcaaa cagaagcaag acggaatact
     2581 tgaggtgatt gagtatcgta ttagccaaat caaggagtta ctgaaatgaa acgcaataaa
     2641 gtagaaagct atactatccg cttattaaaa aataatcata caaaacgcta cttcggagaa
     2701 gttatattaa acggcataaa agaaatcagc acggcagaga tgatgtctga aattaacgct
     2761 attaaggcat taaataagcg tattgaagct ctgaataaaa ccaaagccgt taatttgcct
     2821 aattatcctg aaatcagagg tcatcagcct attatgtaca attacaattt tgaacaaatt
     2881 gaggaagatc ctgaacaggt taagcctaaa ccatcaaaac caaaacgcaa acctttcaca
     2941 ccctacgggc taaacggcta ctttgtggat agcaacggca atattcgcct gcacctagac
     3001 agacgagcaa gtgccgaaac aattaccctt gaaactgatt tcttcaacgc tcttgccgaa
     3061 atggtaaaac gtacacaaga ggcaaacaat cctaaacatg cttagacaat ggattacata
     3121 cggcttatac gccctgcttt ttatggttgc catcaccctc ctaatgcaat gaagagctta
     3181 tccctgaaag cacctgcaag cctgaaactt gcgaatttaa agtaacggag aaagtgtgat
     3241 gataggatac gaagactaca cagatgcttc acttcaacca gaagaagacc ctagcgaaga
     3301 ggctttcctc ttagtggagg agaaaattat agatgtgcta gaaaacaacg gctttgaaac
     3361 cgttgctact atcctagccg gttctgatgg ctggtgtaac gcaattaatg cctacctggc
     3421 cgaacagaac aaacacagaa atgaatttta gaggaataag ataatgacaa accaaaatca
     3481 acaagtagca caaaagcaag ataaattccc gattaaaacc ctatttgaaa gcccgaatgt
     3541 aaagcaaaaa ctacaagagc ttttaaacaa aaacgcagcc acgtttacaa caagcgtgtt
     3601 gcagattgtg aatagtaact caatgttacg caatgccgaa ccgatgagcg tattcaacgc
     3661 cgcttgtatg gcagcaacgc ttaatttggc attacaaaac ggcttaggtt ttgcctacat
     3721 tgtgccgtac caaaaccgca aagagaaaaa aaccgaagct caattccaac ttggctacaa
     3781 aggtttaatt caactggctc aacgtagcgg gcaatttaaa cgccttgttg ccgtgccggt
     3841 gtatgaaaaa caactgcttg ccgaagaccc catcaacggt tacgagttcg attggaaaca
     3901 aaaaccggct aaagatgaaa aaccgattgg ctattacgct tatttcaaac tgataaatga
     3961 atttaccgcc gaaatctata tgaccacgca agaagtccac gaccacgcca accgctacag
     4021 ccaaacctac cgcaaaggct acggcgtatg gcacgacaac tttgaggcaa tggctctcaa
     4081 aactgtgatg aagctcttgc tttccaaaca agctccatta tcagtggaaa tgcaacaagc
     4141 ggtcttggca gatcaagcgg tcgtaaaaga tgttgaaaat gcagaatttg cctaccctga
     4201 caacgaaatc caagaggcgg aatttaccga actcaaggtc agcgatgagg tgtttgagaa
     4261 atgcaaacaa accatcatca acaaagaaag caccttgcaa gatatgtgcg ataacggcta
     4321 cgagttcaac aaagagcaat atgcggagtt ggagaagtta gaaaatgaac agcaatcaaa
     4381 tgtataaatt caaaccccgt tgctcaatgc tccaccaact aatgagcgaa cctcgcacca
     4441 aagccgaccg tgaagccgga ctgatttccg acaccgcaaa agcagcggtg cgtgaagtgg
     4501 ctaaatttga cctattcggt tatcaatcct ttgacggcaa caaatacact gaaaaaggca
     4561 atcagctaga agaacaagcc attaagttaa gcggttttac tcgtggcctg gttctaaaaa
     4621 agaacaccga acgccgagaa aacgattgga tttcaggcga gtgtgatatt tatgtaccaa
     4681 gccgaaagct gattatcgat acaaaatgca gttgggacat cggcacgcac ccgttttttg
     4741 cagacgaagc catcgccaaa gccgaaaaag ccggatatga cattcaaatg caaggctata
     4801 tgtggctgtg ggattgcgaa gaggctcaaa tcgattttgt gttattccct actccaatcg
     4861 accttatcgg tacttacgaa agcacagaaa agctgattga ccttgtggaa caaatccccc
     4921 aacaaaaacg cattaccacc gtgacggtta agcgtgatga agccatcatt gccaaaatca
     4981 aagacagagt gaaagcagcc agtaagtatt acgacaagct tatacaagaa gctatgaatt
     5041 aattcaatcg ccccgaaagg ggctttttaa taggtgacaa atatgacaac ccaaaagaaa
     5101 tacgaactcc tccaaaacga cacaatcaac cacaacggca gaacactcta ccgcatcaaa
     5161 gccataacca catttggaac agtctttgca ggtacacttg gcggctatat tgaaaaagag
     5221 agcaacctaa aacatttagg aaatgcttgg gtgtatgaca acgccaaagt gtatgacagc
     5281 gccgaagtgt atggcaacgc caaagtgtat gacagcgccg aagtgtatgg caacgccgaa
     5341 gtgtggggca gcgccgaagt gtatggcaac gccaaagtgt ggggcaacgc cgaagtgtgg
     5401 ggcagcgccg aagtgtatgg caacgccgaa gtgtggggca gcgccaaagt gtggggcaac
     5461 gccgaagtgt ggggcagcgc cgaagtgtat ggcaacgcca aagtgtatga cagcgccgaa
     5521 gtgtggggca gcgccaaagt aagatcattt gcagttcttt ctgaaagaaa aatgattttc
     5581 tgggcgagca atgtcggcac agagaacggt acattaaccg tttacaacgg aaaagacgga
     5641 ctgcttgtaa caagaggctg tttcaccggc acagtcgaag agtttttagc aaaatccgac
     5701 gaagttcacg atgagaaaac gaagcgggaa tatcagctac tgattgaggt agcaaaaagc
     5761 agaattttag gagaagcgac ttaatcagtc tcttttttat
//
LOCUS       DANLXQ010000150         5770 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.150, whole genome shotgun sequence.
ACCESSION   DANLXQ010000150 DANLXQ010000000
VERSION     DANLXQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.150"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(123..353)
                     /locus_tag="PXD94_002187"
     CDS             complement(123..353)
                     /locus_tag="PXD94_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287007.1"
                     /translation="MALHRCPECRKRISDSLEHCIHCGFSFKEADLAIYRQKLEQRRL
                     HNQEINRKSVKLQLIWLVIFIAVIALASWITN"
     gene            complement(361..1104)
                     /locus_tag="PXD94_002188"
     CDS             complement(361..1104)
                     /locus_tag="PXD94_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287008.1"
                     /translation="MLNKHINKIINSSFSLAFFTLTSFATAYCYGWGQAIFHGYPWWH
                     VEIGNASMARSLAYVFGTSVILFLCYALGYSLLNKVFQLHYFRNLGWLRVIVLVSVFT
                     VPVILTFYLFTGIVPIYISFIYIFVSGVSVFFFRKRWNNTKFDLDFRKMLLGGSFALF
                     NIFIFLYFSLLSLHIGYLRAGLRTTYDYIEIENQKYYILSTNSDNGYIMGTKIRGNQS
                     FLFFNRDTHQYYHVHIEQCTDSRLCKVIV"
     gene            complement(1275..1613)
                     /locus_tag="PXD94_002189"
     CDS             complement(1275..1613)
                     /locus_tag="PXD94_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6287009.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
     gene            1872..3770
                     /gene="dnaK"
                     /locus_tag="PXD94_002190"
     CDS             1872..3770
                     /gene="dnaK"
                     /locus_tag="PXD94_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL6287010.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGDKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFQKEQGVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADATGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALADAGLSVGDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     AEVEQMVRDAEANAEADKKFEELVQARNQADGIAHATRKQITEAGDALNADDKAKIEA
                     AISELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKAQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            3912..4214
                     /locus_tag="PXD94_002191"
     CDS             3912..4214
                     /locus_tag="PXD94_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287011.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATETENSNTAILMDDF
                     VWFEQIPTTEALLADTWSVIENRSKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            4437..5576
                     /gene="dnaJ"
                     /locus_tag="PXD94_002192"
     CDS             4437..5576
                     /gene="dnaJ"
                     /locus_tag="PXD94_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL6287012.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGGGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQFEESLEGQGKHRPKHEGFFDGVKKFFDNLGK"
BASE COUNT         1881 a         1122 c         1223 g         1544 t
ORIGIN      
        1 ttttttatga aaaatcttat aatattgaat atttactcca gttttaatcg agaattatgt
       61 tcttttttat tttgttattc tctttaggta taaaaaaacc cggtaaatac cgggctttta
      121 tttcaattgg ttatccaact tgcgagagca attaccgcaa taaaaataac taaccaaata
      181 agttgtaatt ttacgctttt gcggttgatc tcctggttgt gtaatcggcg ctgttctaac
      241 ttttgccgat atatcgctaa atcggcttct ttaaacgaga agccacaatg tatgcaatgt
      301 tctaaactgt cgctaatacg ctttcgacac tccggacaac ggtgtaatgc cataattacc
      361 tcaaactatt actttgcata atcggctatc cgtacattgt tcaatatgaa catggtagta
      421 ttgatgagta tcacgattga aaaacaagaa agattgatta cctcttattt ttgttcccat
      481 aatgtagccg ttatcactgt tggtggataa tatgtagtat ttttgattct ctatttcgat
      541 gtaatcatag gtagttctta gtccggcacg caaataacca atatgcaatg agagcaaact
      601 gaaatagaga aaaataaaga tgttaaacag ggcaaaactt cctcctaata gcatctttct
      661 aaagtccaga tcaaacttgg tattattcca tctcttgcgg aagaagaata cgctcacacc
      721 acttacaaaa atatagataa aagagatata aatgggcact atcccggtaa ataagtaaaa
      781 agtaagaata acaggcactg taaacacaga aaccaacaca atcaccctta accaacctag
      841 atttcggaaa taatgcagct gaaaaacttt atttagtaaa ctataaccta gtgcataaca
      901 caagaataag atgacggaag taccgaatac ataagccaaa cttcttgcca tactggcatt
      961 gccgatttca acatgccacc aagggtagcc atgaaatatg gcttgtcccc aaccgtaaca
     1021 gtaagcggta gcaaaactag ttaatgtaaa aaatgcaaga gaaaatgaag aattgataat
     1081 tttattaatg tgtttattta gcataacaaa gaatgtctat ttccattaaa acctaactat
     1141 ttttgattac tataataacc cgtattacac aaaaaacaaa catttaattc acaccaaatt
     1201 agaaaatttg ctttagtaag gttgaaaata taactctaaa acacgataat aaaaagcttc
     1261 tctgctagca atggctagac tgcttcatcg tcagtttcgc cggtacgggt gcggataact
     1321 tgttcaatat tcgctacata aattttgcca tcacccactt gcccagaata ggatgcattg
     1381 agaatggcat cgatcacttc atcggtttgg taattagaaa ccaatatctc caccttaatt
     1441 ttaggacgaa agtcaatgcg gctcatttgg ctaagctctt gttcggtatg cccatattgg
     1501 cgtccaaagc cttttacttc ggtaatagtc attccttgaa taccgatttc tgaaagtgca
     1561 tcttgcactt ggtctaattt tgaaggttgg ataatggtag taatcagttt catattatat
     1621 ccttctctgt ttgagttgcc gtaagtataa aaagctattg aggggtatgc aaggtaggag
     1681 gtattttctc cgtgaaatat tctgccaaat ctcccccaca tacaagcggt tactttttcc
     1741 aaaaattttg caattttttt atttttgccc ttgaacagtg cattttcgtc cctatttatg
     1801 atttcgtctg aacgggaaat gcaatcccgt ttcttattca aactgaattt attattatag
     1861 gaatcataaa aatgggtaaa atcattggta ttgacttagg tacaacaaat tcttgtgttg
     1921 cggtaatgga cggcgacaaa cctcgtgtaa ttgaaaatgc ggaaggggca agaacaacgc
     1981 catctatcat cgcttacacc gataaagaaa ccttagttgg tcagccggca aaacgtcagg
     2041 caattactaa cccgaaaaat actttatttg caatcaagcg tttaatcggc cgtcgtttta
     2101 ctgatgctga agtgcaacgt gatattgaaa ttatgccgtt tgaaatcagc aaagcagata
     2161 acggcgatgc gtgggtaacc gtaaaaggcg ataaattagc accgccgcaa atctctgctg
     2221 aaatcttgaa aaaaatgaag aaaaccgcag aagatttctt gggcgaaccg gtaaccgaag
     2281 cggttattac tgtgcctgca tactttaacg atgcacaacg tcaggcaact aaagatgccg
     2341 gtcgtattgc aggtttagat gtaaaacgta tcatcaacga accaacagcg gcagcgcttg
     2401 cttatggttt agattctaag aaagagaacc aaacgattgc tgtttacgac ttaggtggtg
     2461 gtacattcga tatttcgatt atcgaaattg ataactttga cggcgagcaa acttttgaag
     2521 tgcgtgcaac caacggcgat actcacctag gtggtgaaga cttcgataac cgtgtaatca
     2581 actacttagt ggaagaattc caaaaagaac aaggcgttga tttacgcaac gacccaatgg
     2641 caatgcaacg tgtgaaagaa gcagcggaaa aagcgaaaat cgaactttct tctgcacaag
     2701 aaactgaagt gaaccttcca tacatcacgg cagatgcaac cggcccgaaa cacttaaaca
     2761 ttaaagtaac ccgtgcgaaa ttggaatctt tagttgaaga tttagtaaac cgttcgttag
     2821 agccgttaaa aaccgcttta gctgatgccg gtttatcagt aggcgacatc aacgatgtga
     2881 ttttagtcgg tggtcaaacc cgtatgccgt tagtacagaa aaaagtggca gacttctttg
     2941 gcaaagaacc gcgtaaagac gttaacccgg acgaagctgt ggcaatcggt gcggcggttc
     3001 aaggcggtgt gttaagtggt tcggtaacag acgtattgtt attagacgtt actccgcttt
     3061 cacttggtat cgaaacaatg ggtggtgtaa tgactacctt aattgagaaa aacactacga
     3121 ttccaaccaa gaaatcacaa gtgttctcaa cagcggaaga taaccaatct gcggtaacta
     3181 tccacgtgtt acaaggtgag cgtaaacgtg cagcggataa taaatcttta ggtcaattca
     3241 accttgaagg tattaaccct gctccacgcg gtatgccaca aattgaagta acctttgata
     3301 ttgatgctaa cggtattatc aatgtctctg caaaagataa aaacactggc aaagagcaac
     3361 aaatcaaaat tcaagcatct tcaggcttaa gcgatgctga agttgaacaa atggtgcgtg
     3421 atgcagaagc gaatgcggaa gcagacaaaa aatttgaaga gttagttcaa gctcgtaacc
     3481 aagcagacgg tattgctcac gcaacccgta agcaaattac cgaagcaggc gatgcgttaa
     3541 acgcagacga taaagcgaag atcgaagcgg ctatttctga gttagaaacc gcagctaaag
     3601 gcgaagataa agccgagatc gaagcgaaaa tcgaagcgtt aatcaaagct tcagaaccgt
     3661 taatgcaagc agcacaagca aaagcacaag gcggcgagca accacaacaa tctcaaaaag
     3721 atgacggcgt ggtcgatgcg gaatttgaag aagtgaaaga taataaataa tcttgcgtaa
     3781 cttgtagggg cgaaatatct ttcgcccctt ttgtgtagat atataccacc tagcacgagc
     3841 cgtgctaggt tacacataat tcagcccctt tggggctgtg caagcggtgc ttttcaaagg
     3901 aaattttaca aatgctaaaa ctctacccta caacggaaaa tgtacagcca ccgttttata
     3961 aaatatacga tcataaaagt ggcgaaacgg tcattgccac agaaacggaa aacagcaata
     4021 ccgcgatttt aatggacgat tttgtttggt ttgagcaaat tccaactacc gaagctcttt
     4081 tggcagacac ttggtctgtg attgaaaacc gttccaaaca aggtttgctc aaacgagctt
     4141 acaccaaaga ttttgaaaaa catttagcag acggcaaagt agaaatttat attgcggtga
     4201 atccgcattg ttaggctttt gatagcgcaa gcgtccacgc ttgtgtcatc ttgagccatc
     4261 ttggctagat cccatttaat tatataaaac tctctcccct tgtgggagag agaccgaaaa
     4321 atatttcgtt cagaaatatt tttcagagag agggggaatt aaccatccta cccctctccc
     4381 acaaggggcg agggcaagcg gtcaaaaaca cagaaaaatt tacaaacgga acaacaatgg
     4441 caaagaaaga ttattacgaa gtccttggac tgtctaaagg ggcaagcgag aaagacatca
     4501 aacgagcata taaacgtttg gcggcaaagc atcacccgga taaaaaccaa ggcagtaaag
     4561 agtcggaaga gaaatttaaa gagattaccg aagcctatga cgtgctgacc gacagcgaga
     4621 aaaaagcgat gtacgaccaa tacggtcacg ccgcctttga acaaggcggt gccggtgcag
     4681 gcggattcgg tggctttggc ggcggtggtt tcggtggttt tgaagacatc ttcagcgaaa
     4741 tgtttggcgg tggctttggt ggcggtggac gtcgccaacg tgtggtacgt ggcgatgatt
     4801 tacgttacga catcgagatc acccttgaag aagctgtgaa aggctgtaaa aaagatattc
     4861 gcattcaaac cttagctgaa tgtgatactt gtcatggtac aggggcggaa gcagggagca
     4921 aagtggaaac ctgttcgcat tgtcacggct ccggcagagt tcgccgtcag caaggtttct
     4981 ttgtgactga ggcagtttgt ccaagttgtc acggtacagg taaacgcatt gaaaaaccgt
     5041 gccgttcttg ccacggcgat ggacgtgtac agaaagcgaa aaacctctct gttaccattc
     5101 cggcaggtgt ggataccggt aatcaacttc gcttatcgag agagggagct gcaggcgaaa
     5161 atggcgcacc ggcaggcgat ttatatgtgg tgattcacgt taaagaacac gatatttttg
     5221 agcgtgacgg ttcgaattta tactgtgaag tgccgattag ctttaccctt gcagctttag
     5281 gcggtgaaat tgaagtgcca actttagacg gcaaattaaa actcaaaatt ccggcagaaa
     5341 ctcaaaccgg taaactattc cgtgtacgag gcaaaggggt aacaagccct cgtggtggct
     5401 acgcagggga tttaatctgc aaagtggtgg tcgaaacgcc tgtgaattta accgaagaac
     5461 agaaagcact attacgacaa tttgaagaga gtttagaagg acaaggtaaa catcgtccga
     5521 aacacgaagg tttctttgat ggtgtgaaga aattctttga taacttaggc aaataaaatc
     5581 ataaaaagta catatttctt aaatatgtac ttttttatta tctattcttt gttgaaatct
     5641 acttctcggc cgttaatttt tacacctaca tactgcaaaa aatagtggaa aaatgaccgc
     5701 ttatcatctt tctcagaata cttgacaatt aaaatcaact atttaaaatt aatatttaat
     5761 agtcattaat
//
LOCUS       DANLXQ010000151         5726 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.151, whole genome shotgun sequence.
ACCESSION   DANLXQ010000151 DANLXQ010000000
VERSION     DANLXQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.151"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            56..3127
                     /gene="fdnG"
                     /locus_tag="PXD94_002193"
     CDS             56..3127
                     /gene="fdnG"
                     /locus_tag="PXD94_002193"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:641..643,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL6287013.1"
                     /translation="MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAK
                     ETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLIHIEGDSDHPVSRGALCPKGAGALDYV
                     NSDRRVHYPEVREAGSSEWKRISWHEAIERIARHMKNDRDENFVTHNEAGLEVNRWMT
                     AGFLAGSACSNETGIVTQKFIRSLGMIFTDNQASIUHGPTVASLAPSFGRGAMTNHWV
                     DIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLMVVDPRFNRSAAVADIYIPLR
                     PGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNEGFKFEDGLFTGYDEATRTYK
                     DKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRYTPEMVERITGTPQKDFLIFC
                     EEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQLLCGNIGVSGGGVNALRGHS
                     NVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAPKTLVAGQVNYGQNNPKFAVS
                     MLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQMYQGKVNGYFCQGMNPIASYP
                     NSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNVNPSEIQTEVFRLPTTCFAEE
                     DGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAELIHLYHKEGGKAPLEPLEAMT
                     WNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKGELLSSFTQMRDDGTTSGGCW
                     IYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNRRILYNRASADLSGKPWNPKR
                     QLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVSGLFVRERMQDGPFPEHYEPF
                     ETPIGTNPLHPNVISSPVARILPSDKEDLGTSDEFPYVGTTYRLTEHFHYWTKNALIN
                     VIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKAVAVVTKRIRSLVSDGKPIHT
                     IGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPESKCMLLNIEKIVGA"
     gene            3130..4044
                     /gene="fdxH"
                     /locus_tag="PXD94_002194"
     CDS             3130..4044
                     /gene="fdxH"
                     /locus_tag="PXD94_002194"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL6287014.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYVLHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            4037..4714
                     /locus_tag="PXD94_002195"
     CDS             4037..4714
                     /locus_tag="PXD94_002195"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL6287015.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            4770..5687
                     /gene="fdhE"
                     /locus_tag="PXD94_002196"
     CDS             4770..5687
                     /gene="fdhE"
                     /locus_tag="PXD94_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL6287016.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
BASE COUNT         1674 a         1244 c         1337 g         1471 t
ORIGIN      
        1 attaattttg atcaaatttt gttttatttc tcacattatg tgatttggag tgattatgca
       61 gatctcaaga cgtaagttct tcaaggtctg tgcaggtggt atggcgggaa cttctgctgc
      121 aatgttgggt tttgctccag caacagcatt agctgcaccg cgtgagtata agctcttaag
      181 agcaaaagaa actcgacaaa cctgtactta ctgcgcggta ggctgtggca tgattatgta
      241 cagcttaggc ggcgaagcaa tgaacagcaa aggaaagtta attcatattg agggtgattc
      301 tgaccatcca gtaagccgtg gggcgttatg tccgaaaggt gcaggggcgt tggactatgt
      361 taatagtgac cgccgtgtgc attatccgga agtgcgtgaa gccggttcaa gtgagtggaa
      421 acgcatttct tggcacgagg caattgaacg tattgcccgc catatgaaaa atgaccgtga
      481 tgagaacttc gtcacccaca atgaagcagg gctggaagta aaccgttgga tgacggcagg
      541 tttcttggca ggctcggcgt gtagtaacga aaccggtatc gtcacccaga agtttatccg
      601 ctctctcggt atgattttta ccgacaacca agcgagtatc tgacacggac caacggtagc
      661 aagtcttgct ccatcatttg gtcgcggtgc gatgaccaac cactgggttg atattaaaaa
      721 ctccgattta gtgatcgtta tgggcggtaa cgcagccgaa gctcaccctg tgggtttccg
      781 ttgggcagtt gaagcgaaaa aacagaacgg tgcgaagtta atggtggtag atccacgctt
      841 taaccgtagt gctgcggtgg cggatattta tataccattg cgtccgggta cggacattgc
      901 gttcttgtta ggtgcggtaa actacctgct taaaaacgac aaaattcaac acgagtatgt
      961 gaagcattac accaatgcca cattcttact caacgaaggg tttaaattcg aagacgggct
     1021 tttcaccggc tacgatgaag ccactcgtac ctataaagac aaatccactt gggcttatca
     1081 gttagatgaa cagggtcagc cgaaacggga tatgacaatg caagatccac gttgtgtgat
     1141 caatatgctc aaagaccacg tttcccgcta tacgcctgaa atggtagaac gcattaccgg
     1201 tacgccacaa aaagatttcc tgattttctg tgaagagatt gcgaaaacct cagcgccgga
     1261 taaagccgcc accttcctct atgccttagg ttggacacaa cacaccgtgg gcgcacaaaa
     1321 cattcgctca atggcgatca ttcagttgct gtgcggtaac attggtgtct ccggcggtgg
     1381 ggtaaacgca ctgcgtggtc actctaatgt acaagggatt accgacctag gcttgttccc
     1441 acatatgttg ccgggctata tccgcctgcc aaccgaagcg gacgcaacct tagaaagcta
     1501 cttaaatgcg attgcaccga aaaccttagt ggcagggcaa gtaaactacg ggcaaaacaa
     1561 cccgaaattc gctgtgagta tgctcaaaac cttctacggc gataaggcaa ccaaagagaa
     1621 cgagttcggc tatcactatt taccgaaata cccgaaaggc ggtacagatc acttccgctt
     1681 tatcgaacag atgtatcaag gcaaagtgaa cggctatttc tgccaaggta tgaacccgat
     1741 tgcctcttac cctaactcgc agaagttaat taaagcgttg agcaatttga aatatttagt
     1801 catttttgac ccgcttatta ccgatacttc cgagttctgg aaaaatcacg gtgagttcaa
     1861 caatgtgaac ccgtctgaaa ttcaaaccga agtgttccgc ttgccgacca cttgctttgc
     1921 ggaagaagat ggttcaattg cgaactccgg tcgctggtta caatggcact ggaaagcagc
     1981 agacgcacca cacgaggcaa aaccggacgt ggatattctg tctgaaatcc gtgccgaact
     2041 gatccacctt tatcataaag aaggcggaaa agcaccgctt gaaccgttag aagcgatgac
     2101 gtggaactat atgaacccgt tagaaccgaa gccggaagag atcgccaaag agaacaacgg
     2161 ttatgcattg gaagatctga aagatgcaga cggcaacatc atcgtgaaaa aaggcgaact
     2221 attatccagc tttacacaaa tgcgtgatga cggtacaacc tccggtggtt gttggatcta
     2281 ctgcggtcaa tggactccga aaggtaacca aatggcgaac cgtgataact ccgacccatc
     2341 aggcttaggt aacaccttag gctgggcatt ctcgtggccg gtaaaccgcc gtattctata
     2401 caaccgtgca tcggcagatt taagcggtaa accgtggaac ccgaaacgcc agctagttaa
     2461 atggaacggt aaaaactgga actatgtgga cgttgccgat tacggtacag caccaccaaa
     2521 tagccctgtg acaccattta ttatgcaacc ggaaggggta tccggcttgt tcgtgcgtga
     2581 acgtatgcaa gacggtccat tccctgagca ttatgaaccg tttgaaacgc caatcggcac
     2641 gaacccgtta cacccgaatg taatcagcag tccggtggca cgcattctgc cgagcgataa
     2701 agaggattta ggcaccagcg atgagttccc atatgtcggt acaacttacc gcttaaccga
     2761 acacttccac tactggacga aaaatgccct gattaacgtc attgctcaac cggagcaatt
     2821 cgtggaaatc ggcgagaaac ttgccgaaga gaaaggcatt aaaaatggcg acaaagtacg
     2881 tgtttggtct aaacgtggcg aaattaaagc cgttgccgtg gtgactaaac gtatccgttc
     2941 attggtctct gacggcaaac caatccacac tatcggtgtg ccgatccact ggggctttgc
     3001 ggctacaacc ggtgcgaaaa aaggctattt tgccaataac ttaaccgtgc gaacaggtga
     3061 accaaactca aatacacctg aatcgaaatg tatgttatta aacattgaga aaattgtggg
     3121 ggcttaacaa tgagtacagt tcaaactcaa aacattatta aaatctccgc aacctcggcg
     3181 ctgacaccgg cgccgcaggc tcgttcgcac caagtggaag tagcgaaact gatcgatgtc
     3241 accacttgta tcggctgtaa agcctgtcaa gtgggctgtt cagaatggaa tgacattcgc
     3301 tccgatgtaa atgcaaactg tgtcggcgtg tacgacaacc ctgcggatct aaatgcgaaa
     3361 gcgtggacag taatgcgctt taacgaagta gaagaaaacg accgtttaga atggttgatc
     3421 cgtaaagacg gctgtatgca ctgtgccgag ccgggttgct tgaaatcttg cccgtcaccg
     3481 ggtgcgatta tccaatacgc caacggtatt gttgatttcc aatccgacaa atgtatcggt
     3541 tgtggctact gtatcgcagg ttgtccgttc aacattcctc gtatgaatcc ggacgataac
     3601 cgtgtttaca aatgcaccct gtgtgtggat cgtgtcaatg ttggtcaaga accggcttgc
     3661 gtgaaaacct gcccgaccgg tgcaatccgc ttcggtagca aagaagagat gaagttctat
     3721 gcagaacaac gcattgctga tctgaaatct cgtggctatg aaaatgccgg cttgtacgac
     3781 cctgaaggcg ttggcggtac gcacgtaatg tatgtattac atcatgccga caaaccggag
     3841 ctttattcag gcttaccgaa agatccgaag attgatacca cagtaacctt atggaaagac
     3901 gttttaaaac ctgtggcggc agtagcgttg ggtggtttag ctttagcaga agttgcacac
     3961 tatattaccg ttgggccaaa cgttgaggaa gatcttgaag aacaccacca tagcgaaaat
     4021 gcagaaggag gcaaacatga gtaagaaatt tgaaatcacg aacgatacca aaatcgttcg
     4081 tcataagccc ttagctcgtg ttagccactg gttcttagta atctcttttt ttatgacgat
     4141 gtttaccggt gttgccttct tcttccctga ctttgcttgg cttaccgaga ttttaggtac
     4201 tccacagatt gcccgtgcag tccacccatt taccgggatt ataatgttta ttgcatttat
     4261 tattatggcg ttaatatatt ggcaccataa tatccctgag aaaaatgata ttcgttgggc
     4321 aaaaggcatt gttgaagtac taaaaggtaa tgagcataag gtttccgata atggaaaata
     4381 caatttgggt caaaaattac tcttttggac tcttattctt gcgatgttca ctcttttagc
     4441 cacagggatt attatgtgga gacaattttt ctctcactac ttctcgattc ctatgctaag
     4501 aattgcaatt ttacttcatt cagcaagtgc atttatgcta tttaccggaa ttttagtaca
     4561 tatgtatatg gcattctggg taaaaggctc aatcagaggt atggttgagg gttgggttac
     4621 cgttcgctgg gcaaaaaaac accacccaag atggtaccgt gaagaagtgc tacctgaatt
     4681 agaaagacaa gtagctgagc aaaataataa ataacctcaa gtgcggtatt attaccgcac
     4741 ttttatttta ttggtttaaa aagaatatta tgagtatcaa aatactacct caagatgata
     4801 tcaaacaagc agctagttca tttcagcaac ccgagctctt atttgctaat cccaaaaatc
     4861 tgtatttacg ccgcgctaag cggctacgtg aattagccaa gcaaaaccca ttcggtgaat
     4921 atatggagtt ttctgctaac ctcgtggaca ttcaattagc attacttgaa agccagccaa
     4981 ttgcagatta ttctcaaaaa ataaccgctt gtgtagagcg cacgcaagga gaaacaccgc
     5041 taaatgcaca gaattttcag cgtactgatg aatggcggac actactctta gccattattg
     5101 agaaattcaa gccttatgca aatgatactg ttcttgcaac aattgaagta ttggaaaagg
     5161 cagctaagtc agagctagac acacttgctg ataatttgct aaatgaacga tacgaacttg
     5221 tgggcgcaga taaagctgtc tttatttggg ctgctctctc actttactgg gtacaattag
     5281 ctcaacaatt accacgtaat tctcgtgcag aaataggcca taaacattta tgtccggtat
     5341 gtaactcagc acctgtaagc agtgtgattc atttcggtga agctcaaggg ttacgttatc
     5401 tacactgttc tttatgtgaa agtgaatggc atgtagtacg tacaaagtgt acaaactgtg
     5461 atgaaaccgg caaactggat tattggagct tagatacagt agaagcagca gttaaagctg
     5521 aaagttgtgg cgactgccat tcctacttaa aagtactcta tcaagataaa gacgtgaatg
     5581 tggaacctgt tgctgatgat ttagcaactc tattcttaga ttccgagatg gaacaaaaag
     5641 gtctttcaag aagtgggata aatccatttt tatttcaagt agagtaactc atttacaaca
     5701 aaaacggagc ataacgctcc gttttt
//
LOCUS       DANLXQ010000152         5726 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.152, whole genome shotgun sequence.
ACCESSION   DANLXQ010000152 DANLXQ010000000
VERSION     DANLXQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.152"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            10..357
                     /locus_tag="PXD94_002197"
     CDS             10..357
                     /locus_tag="PXD94_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287017.1"
                     /translation="MFREEAIEYQSKKWKSTAVLFSRVPALVVFSISFFIFTAFILFI
                     VFGNYTRRETVVGELVMQSHPILLSAPKSGYISENYIQPHQQVKKRAIRWLKLRWIEL
                     RIAKILMLILFFR"
     gene            354..1271
                     /locus_tag="PXD94_002198"
     CDS             354..1271
                     /locus_tag="PXD94_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL6287018.1"
                     /translation="MKSQIQATEQAITALYKNKSETINSLEKQIKNSQKIYQDKQSYL
                     VEIEKSMNDYAELVKRYEKLLKVGHSSHDEVNIQKSRYFQQKSLFNELKQELIQLKST
                     QLSLENEIETRKTEFNNQIIRYEMQKSDLNIRLMEFESVSDIIVNASLDGKIESTSVT
                     VGQIIKENDPLAQILPQNKGNYQLVMWVPNSAISFIKQGDEVNIRYEAFPFEKFGQFK
                     GKITSISTLPASLQELSFYKNLPVNLEQGIPLYKILIELADQNVKYNDTSLYFMSGMK
                     AEATLFLEKRKLYEWIFFPMYQLRKNMEQ"
     gene            1271..2062
                     /locus_tag="PXD94_002199"
     CDS             1271..2062
                     /locus_tag="PXD94_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HDL6287019.1"
                     /translation="MTGIKQLKFDFRRRIPVILQTETAECGLACLAMMLGYYDKNTNL
                     FELRSQYGTSSRGVTLHTLISIANDCGLITRPLSLELDEVPQLRLPCILHWDFNHFVV
                     LTHASEKQFIIHDPAFGKRKLSRAEFSNHFTGVALEVWSEVKFEKSNNENTISLYETL
                     KHISGIKGALVKIFALSMLIELIALLMPIGTQLVMDHVMQAKDQSLLLIICIGLFLFT
                     LFRTMVSMFRAWVSLKMNYLIDFQWTASFFSHLLKLPLDFFENAK"
     gene            2440..3357
                     /locus_tag="PXD94_002200"
                     /pseudo
     CDS             2440..3357
                     /locus_tag="PXD94_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3359..4027)
                     /locus_tag="PXD94_002201"
     CDS             complement(3359..4027)
                     /locus_tag="PXD94_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL6287020.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(4071..5438)
                     /locus_tag="PXD94_002202"
     CDS             complement(4071..5438)
                     /locus_tag="PXD94_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL6287021.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(5557..5652)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT         1760 a         1024 c         1153 g         1789 t
ORIGIN      
        1 tggtattata tgttcagaga agaagctatt gaatatcaat cgaaaaaatg gaaaagcaca
       61 gctgtactat ttagcagagt tccagctttg gttgtctttt ccatttcttt ctttatcttt
      121 actgccttta ttctatttat tgtgtttggt aattataccc gccgtgaaac ggttgtgggt
      181 gaattagtga tgcaatctca tccgatttta ttatctgccc caaaatccgg ctatatatcc
      241 gaaaattata ttcaaccaca tcagcaggta aaaaaaaggg cgatccgctg gttaaaatta
      301 cgctggatag aattacgcat agcgaaaata ttaatgttaa ttctattctt tcgttgaaat
      361 cacaaattca agcaacagaa caagcgataa ctgctttata taagaataaa tcagaaacta
      421 ttaatagcct tgaaaagcag atcaaaaaca gtcaaaaaat ttatcaagat aagcagagtt
      481 atttagtgga aattgaaaaa agtatgaatg actatgcgga gttggttaaa cgctatgaga
      541 aattattaaa agtagggcat tccagccacg atgaagtgaa tattcagaaa agccgttatt
      601 tccaacaaaa atcactgttt aatgagctaa agcaagaatt aattcaatta aaatccaccc
      661 aacttagctt ggaaaatgaa attgaaaccc gtaaaaccga atttaataat cagattattc
      721 gctatgaaat gcaaaaaagc gatttaaaca ttcgtttaat ggaatttgaa tctgtttcag
      781 atattattgt taatgcctct ttagatggaa aaattgaaag taccagcgta acagttgggc
      841 aaattattaa agaaaatgac ccgcttgcac aaattctgcc acagaataaa ggaaattatc
      901 agctagtgat gtgggtaccg aatagtgcca tttcatttat taaacaaggc gatgaggtga
      961 atattcgcta tgaggctttt ccgtttgaaa aattcgggca gtttaagggc aaaattacgt
     1021 caatttcaac cttaccagct tctttgcaag aacttagttt ttataaaaat ctccctgtaa
     1081 atttagagca aggtattccg ctttataaaa ttctgattga gcttgccgat caaaacgtaa
     1141 aatataatga tacctccctt tattttatga gtggaatgaa agccgaagcc acgttatttt
     1201 tagaaaaacg taaactgtat gagtggatat ttttccctat gtatcaatta cgcaaaaata
     1261 tggagcaata atgacaggaa ttaagcagtt aaaatttgat tttcggagaa gaatacctgt
     1321 tattttgcaa actgaaacag ctgaatgtgg cttagcctgt ttagcgatga tgttgggcta
     1381 ttacgataaa aacaccaatt tatttgaatt gcgaagtcaa tatggtactt cctctcgtgg
     1441 cgtaacgctg catactttaa tttctattgc aaatgactgt ggtttaataa ctcgccctct
     1501 ttctctagag ttagatgaag taccacaact tcgtctgcct tgtattcttc attgggattt
     1561 taatcatttt gtagtattaa ctcacgcaag tgaaaaacag tttattatcc acgatcctgc
     1621 tttcggtaaa cgtaaattat cccgtgctga attttccaac cattttaccg gagtcgcttt
     1681 agaagtttgg tctgaagtta aatttgagaa aagcaacaat gaaaatacca ttagtttgta
     1741 tgaaacctta aagcatattt ctggcataaa aggtgcatta gtgaaaattt ttgccctttc
     1801 aatgctcatt gaactcattg ccttattgat gccaattgga acacagttag tgatggatca
     1861 cgttatgcaa gcgaaagatc aatcattact tttaattatc tgtattgggc tatttttatt
     1921 tacccttttt cgcacaatgg tcagtatgtt tagggcttgg gtttccttga aaatgaatta
     1981 tttaattgat tttcaatgga cagccagttt cttttctcat ctcttaaaat taccgcttga
     2041 tttctttgaa aacgccaagt aggtgatatt cattcccgat ttagttcatt aaaaaccatt
     2101 cagaaaactc taacttcaag cattattacc acgattattg atacgattat gataatcagt
     2161 ttaattatta tgatggtgtt atatggcggt tggctctttt gggtagtatt agctttttcc
     2221 tttatctatt ttattcttcg aatggttact tatttagttt atcgtcaact gaatgaagag
     2281 cagattatta aaggagcaaa agcccattcg cattttatgg aaactttata tggtattaca
     2341 acgttaaaat cattaggatt ggaaaataaa cgacaagaac agtggatgtc gctcaatgcg
     2401 gatgctttta acacatcaat taaggtaact aaatttgata tgctgttttc cggtattcat
     2461 acctttattt ctaccatcga acagatttta attctttgga ttggggcgac aatgatgatt
     2521 gaaaatgtga tgacattagg aatgtttgtc gcttttaatg cctatcgagg ctctttctct
     2581 tcacgaatgg ggaatttaat tgatattatt tttagcctaa aaattctttc attacataga
     2641 gagcggattg cagacattgc tttaaatgaa gtcgaagatg aaagtaaata tcaagaactc
     2701 agatttgaac ataaccaaac agatatagaa gttaaagacc tttgtttcaa atacgatcct
     2761 ttttctaaaa atgtgcttga aaatgtcaat ttagtgatca gaagtgggga aagtgttgct
     2821 attaccgcac catcaggtta tggcaaaaca acgttattaa aattaattgc aggtttatta
     2881 aaaccaacct caggttctgt gtattttaac ggcattgata tttatcagtt aggattaagt
     2941 caatatcgga gccaaattgc ttgcgtatta caggaagata aattcttttc tggttccatt
     3001 ttagagaata ttgttagttt tgaaagtaat tatgatcgag aatgggctat cgaatgtagc
     3061 aaacttgcga atattcacaa tgatattatg gcaatgccga tgaattatga aaccttatta
     3121 ggagaattag gaaataattt gtctggcgga caaagataac gattgtttat tgctcgagct
     3181 ttatataaaa agcctaaaat tcttttgatg gatgaggcga ctagccattt agatgaagaa
     3241 aatgagaagc ttgtcaatca agctatttca cgaatgaaga taacaaggat tattattgct
     3301 catagacaat cgactattca gtcggcggat agaattattg ccctgaatga caggtaaatt
     3361 aattcctatt tttctgttct tgccgataaa gtttattgca cggaatgctg tcgttgttgc
     3421 cgtctagctc tttccagccg cattgttgaa agtagcgttt tgcctgtgcg taattgccga
     3481 tttggttgca gcttaattta atttggcaat caatcgttgg tggctggttt gcaatatttt
     3541 ccggtttttt gaccgcttgt ggtaaggttg aggtgtgtgt ttttcgccat tggctcgggt
     3601 taatcgggaa tttgtcctgc cataagccga ttttagcttg ctgtgcttga tcttgtgctt
     3661 cgatatattc ccgctttgtg gtgtaagccc acgccatacc gttttccact aaggctaagt
     3721 tgatgttttt atcgtttaag tagagggtgg caagtaatcg ttggtaacga tcatagcctg
     3781 aaactatggc agtaatgttt tgttgaaaca cgaattgagc cagagcttgt ttggctttgt
     3841 tgccgtaagg ctgcgcttgt tcgggggcat caatatgttg tagccttatt tttagtggtg
     3901 ttttattgac caaacaagtg agggtgtcgc catcgctaat gccaacgact ttgcattgta
     3961 gttggcgttc ggcggcatat accgatgaga aaaataagct aaaaatcagg gcgtaaagat
     4021 atttcattat taaaaaagag agggctaata agccctcatt ctatcatttt ttattcggct
     4081 ttacctgttt taaggcgagc gaagaaatct tttgtttcac taataatcac tttgcgtaac
     4141 gcaatcaatg cgattaaatt cgggaatgcc attaagccgt tcacgatgtc tgccagcgtc
     4201 caaattgtat cgagttgtaa aaatggagca gaggcgatga gtgcaataaa cagcaagcgg
     4261 tataatttaa tgtattgggt gcgacctcga gtgagataga caaaacagcg ttcaccgtag
     4321 tagcaccagc ctaaaatggt ggtaaaggca aagaagacta aaccaactgt cacaattatc
     4381 gcaccgaatg agctgtttaa accttgagta aaagcggttg tggtgagttc tgcgcctttt
     4441 acatcgcctg cccaagcccc tgaaatcaca attactaagc cggtcattgt gcagacgata
     4501 atagtgtcta agaacgtgcc ggtcattgaa attaggcctt gacgtaccgg ctctttggtt
     4561 tgggcggcag cggcggcgat gggggcagag cctaatcctg attcattcga gaaaatacca
     4621 cgtgcaaccc ctaattgaat cgcttgcatg acggtgaatc cgactgctcc gcctaatgcc
     4681 gcttgtgggt tgaaagctgc gtgtacaata gtaataattg cttgtgggac tttttccata
     4741 ttcagtagaa tgacggataa agcggcgatg acatacccaa tagccataaa cggtacaaca
     4801 atggttgcaa ttttagaaat ccgctgcacg ccacctaaaa taatagcggt aacgataagg
     4861 gttaaaatga tcgcactgaa gataatcggc acatcaaagg tgctttctaa ggcgtgggta
     4921 atgccattca cttgggggaa cgtgccaata ccaagcaaag cgaccaatac gccaaaaaac
     4981 gcaaaggctt tagcaagcca acgccatttt tttcccattc ccagttcgat atagtacatc
     5041 ggaccaccag ccacaaatcc ggctctatct ttagttcgat atttaattgc caataagcct
     5101 tcggcatatt tcgtagccat accgaataag gcaatcagcc acatccaaaa taatgctcca
     5161 ggcccgcccg aatgaatagc ggttgccacg ccgacaatat tccccgtgcc gatagtagct
     5221 gctagggcag tggaaagggc tgcaaaagcg gaaatatcgc ccgattgccc tttgcctttt
     5281 tccggtttga acatatagcg aaaggcttgc cctagttggc ttaattgaat acctttgagt
     5341 gaaaaggtta aatataaccc tacgcctgaa attaaaatca ggagtggcgg gccccaaaca
     5401 aaactgttga tttgattaag aatagtgtcg atagacattt gatgttcctc gtaaaaactg
     5461 cttacaagga aagaaaagga atgcaagaga caagcggtca aaaaacggaa attttttgca
     5521 aagaattagc aaaaaaacac cgcttgtatc gtataacttt tctcccctgt ccttttgcct
     5581 gagcgtttca cacattgctg tattttcgcc ttcggcggcg gattttaatc cgctctctcc
     5641 aagggttcgt ccagtaacag tccccgcagt gttcactgca cctgaaagag tttacctcgt
     5701 cggtgaaatg ttaattcatt tctctc
//
LOCUS       DANLXQ010000153         5694 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.153, whole genome shotgun sequence.
ACCESSION   DANLXQ010000153 DANLXQ010000000
VERSION     DANLXQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.153"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1037
                     /locus_tag="PXD94_002203"
     CDS             <1..1037
                     /locus_tag="PXD94_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL6287022.1"
                     /translation="LFGTKKEKKTFKFSKIESVLLKPIGDAIGDAVVHIAHLEQIKKA
                     LPNVKIAVLVTSRNKLIFSQANVVDMILEDKVSTYLSQRGHWDLYLDFKPTFTTKSII
                     LDSILKPKFIINFGKKQKSIYNLDTVKNYDFTTVFPKQVHFADYLNYAIFADFLEKNQ
                     VNYNLNIPNELSSFANTLWQEKKIRLLLNPQGSMREISTQELSELLSMIDKRYVQHIS
                     CLLTNTTNSAEYLSKIKAPFEISLAPNSDILGYCALVNSADIVIAVDGGGVHISCALG
                     KKLLSFYANHPANIARWYPKPKKDVETFMVIADKWTEDNNDTRGFSLVAPAKWLNHQF
                     AKLIEKYTAC"
     gene            complement(1092..1304)
                     /gene="rpmE"
                     /locus_tag="PXD94_002204"
     CDS             complement(1092..1304)
                     /gene="rpmE"
                     /locus_tag="PXD94_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL6287023.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
     gene            1662..3668
                     /gene="tkt"
                     /locus_tag="PXD94_002205"
     CDS             1662..3668
                     /gene="tkt"
                     /locus_tag="PXD94_002205"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL6287024.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIEDLKQFRQLHSKTPGHPE
                     YGYAPGVETTTGPLGQGITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDG
                     HNPEQIKFAIENAQAETERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREF
                     LGWEHAPFVIPAEIYTEWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELP
                     ANWEAESKAFIEKLQANPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSG
                     SKPIRAEHNVDGNYINYGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMA
                     ALMKQRALFVYTHDSIGLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAA
                     VERKDGPTALIFTRQNLAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEV
                     DLAMKAADVLDAEGVKVRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFW
                     YKYVGFDGRIVGMNSFGESAPADQLFKLFGFTVENVVAKAKEIL"
     gene            complement(3794..4864)
                     /locus_tag="PXD94_002206"
     CDS             complement(3794..4864)
                     /locus_tag="PXD94_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL6287025.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRSEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(4879..5604)
                     /gene="fadR"
                     /locus_tag="PXD94_002207"
     CDS             complement(4879..5604)
                     /gene="fadR"
                     /locus_tag="PXD94_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL6287026.1"
                     /translation="MGHSDILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAER
                     IGVTRTTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVI
                     ANVVSLRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLA
                     NKPVYALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAK
                     NRQSSGEIWQKLSANIPESFGQL"
BASE COUNT         1744 a         1164 c         1086 g         1700 t
ORIGIN      
        1 aactttttgg tacaaaaaaa gagaaaaaaa ccttcaaatt tagcaaaata gaatccgtat
       61 tactcaaacc tatcggagat gctattggcg atgctgttgt gcatatagct catttggagc
      121 aaattaaaaa agctctccca aatgtaaaaa tagcggtttt agttacatct cgtaacaaat
      181 tgattttctc tcaagcaaac gtggtagata tgatcttaga agataaagta tccacttatc
      241 tctcgcagcg gggacattgg gatctttatt tagatttcaa accgacattt accacaaaaa
      301 gtattatttt agatagtatt ttaaagccta aatttattat taacttcggt aaaaaacaaa
      361 aaagtattta caatttagat acagtaaaaa attacgattt caccactgtc tttccaaagc
      421 aggttcattt tgctgactat ctcaactatg ctatttttgc tgatttttta gaaaaaaatc
      481 aggtcaatta taatttaaac attcctaatg aactaagcag ttttgcaaat acgctatggc
      541 aagagaagaa aattcgatta ctattgaatc ctcaaggtag tatgcgtgaa atttctactc
      601 aagaattatc agagctatta tctatgattg ataaaagata tgttcaacat attagttgtt
      661 tgctgaccaa tacaaccaat agtgccgaat atttgtcgaa aattaaagcc ccatttgaaa
      721 taagcctagc acctaatagt gatattttag gttattgtgc attagtaaac tctgctgata
      781 ttgtaattgc agttgatgga ggtggtgtcc atatttcttg tgcattaggc aagaaattgc
      841 tctcatttta tgccaatcat ccagctaata ttgcacgctg gtatccaaaa cctaagaaag
      901 atgttgaaac ttttatggtg atcgcagata aatggaccga ggataataat gatactcgag
      961 gtttttctct ggtagctcct gcaaaatggt taaatcacca atttgcaaaa ttgatagaaa
     1021 aatacaccgc ttgttaattc gatatataag aaaaacaaaa ccccgcatta gcggggtttg
     1081 caataatcaa attattttga gcttgggatg ctgaaacgtt tgttaaagcg ttcaacacga
     1141 ccaccggtgt caacaacacg ttgtttacca gtatagaacg ggtggcagtt gccacatacg
     1201 tctaagttta aatctttacc cactgttgag cgagttttaa ctacattacc gcaagagcaa
     1261 gttgccgtaa tttctttata ttctgggtga ataccttgtt tcattgggga acctcataag
     1321 aagccatatc gccgcctatc ttgttagaat ctctaccgct gataggtacc atatgtaatt
     1381 aataaaaatg ttgagcaaat gctcaacgcc aaaaagagtg cgtattttag ataaatttgt
     1441 tgcctagatc aactactaaa cattcgcttt atcaagaaaa tattgtatta ggaaaaaacc
     1501 gtctattaaa atccaatcaa aacaaatcac aatttaatca ttttttgtga tacagatcac
     1561 atttctcatt tccatttttg agtttttcta gcaaaatagt gtagaatcca attcgatatc
     1621 ctttccattt tatataacat tctcgattag gagtaacaca aatgacagaa cgtaaagtgc
     1681 tagcgaatgc aattcgtttt ttaagtatgg atgctgtaca aaaagctaac tcaggccacc
     1741 caggcgcacc aatgggtatg gctgatattg cagaagtttt atggcgtgat tttttaaaac
     1801 acaatccaac taatcctaaa tgggcagatc gtgaccgttt tgttctgtct aacggacacg
     1861 gttcaatgtt aatctatagc ttacttcatt taaccggcta tgatctctct attgaagatt
     1921 taaaacagtt ccgtcagtta cattctaaaa cacctggtca ccctgaatat ggttatgcac
     1981 cgggtgttga aaccaccaca ggcccgttag gtcaaggtat taccaatgcg gtcggtatag
     2041 cgattgcgga aaaaaccctt gcagcacaat ttaaccgtga aggtcataaa attgtcgatc
     2101 actacactta cgctttctta ggcgatggct gtttaatgga aggtatctca cacgaagctt
     2161 gctctctcgc cggtacatta ggcttaggca aactgattgc tttctacgat gataacaata
     2221 tctctatcga cggccacgtt gatggttggt tcactgatga taccgcacaa cgctttgagt
     2281 cttatggctg gcaagttatc cgtaatgtcg atggccataa cccagaacaa atcaaatttg
     2341 cgattgaaaa tgcacaagcc gaaaccgagc gtccaacttt aattatttgt aaaaccatta
     2401 tcggctatgg ttcaccaaat aaatcaaact ctcacgattg ccacggtgcg ccattgggtg
     2461 atgctgaaat ccaagccgct cgtgaatttt taggttggga acatgcccca tttgtgattc
     2521 cagctgaaat ttatacagag tgggatgcaa aagcaaaagg tacattagca gaaaaagatt
     2581 ggaacgctaa atttgctgct tatgaagcgg cataccctga actagccgct gaatttaaac
     2641 gccgttacgc aggcgaatta ccggctaact gggaagctga aagcaaagct tttattgaaa
     2701 aattacaagc aaatccggca accattgcaa gccgtaaagc atcacaaaat gcaatcgaag
     2761 cttacgcgcc aatcttacct gaattcctcg gcggctcagc tgacttagca agctctaact
     2821 taaccttatg gtctggctca aaaccgattc gtgcggaaca caatgtagat ggtaactaca
     2881 tcaactacgg cgtgcgtgaa ttcggtatgt ctgcgattat gaacgggatt gccctacacg
     2941 gtggtttcat tccttacggt gcaaccttct taatgttcta cgaatatgca cacaatgcgg
     3001 tgcgtatggc agccttaatg aagcaacgtg ccttatttgt ttatacacac gactccatcg
     3061 gtttaggtga agacggcccg acccaccaac cggtagaaca aactgcttca ttacgccaaa
     3121 ttccaaactt agaaacctgg cgtccggcag accaagtaga atctgctgtt gcttggaaag
     3181 cggcggtaga acgtaaagac ggcccgacag cactaatctt tacccgccaa aatctggctc
     3241 aacaacaacg tactgcggag caattagcga acatctctcg tggtggctat atcttgcgtg
     3301 aatgttgcga aaaaggcagt tgcccggatt taatcttaat tgcaaccggt tctgaagtag
     3361 atttagcaat gaaagccgct gatgtgttag atgcagaagg cgtgaaagtg cgtgtagttt
     3421 caatgccaag caccaacgtg tttgacaaac aagatgaagc ctatcgtgaa tcggtattac
     3481 cacgttcagt cactaaacgt gtggcgattg aagctcaatt agctgacttc tggtataaat
     3541 atgtcggctt tgatggtcga attgtaggta tgaatagctt tggtgaatcc gcaccagcag
     3601 atcaattatt caaactcttc ggctttaccg ttgaaaacgt ggttgctaaa gcaaaagaaa
     3661 tcttataatg aaacaataag ataaagaaaa gcgggaatta acttcccgct tttttgttga
     3721 acaactgtta tgataagcgg tgaaatttca gcgatttttt gcaaaaaatt agcgaaatat
     3781 gaccgcttgt aagctataac atttcagctt gagcaagaat tttttcttgc tctaaagcta
     3841 atttttccag ttttcgctgt tctttacgct cttcatcaac cgcttcccac gcatcatagg
     3901 cttgcacaat tttggcaacc acagggtggc ggacaatatc tttgctgtca aagaaattaa
     3961 agctgagttc cggcacttct tttagcactt ccattgcgtg ttttaagcct gatttttggc
     4021 tacggggcaa atccacttgg gtaatatcgc cggtaatcac cgctttggaa ttaaagccaa
     4081 tacgggttaa gaacattttc atttgctcgg tagtcgtgtt ttggctttca tctaaaatga
     4141 tgaacgcatc gtttaaagta cgccctcgca tataagcaag tggggcaatt tcaatcacac
     4201 cacgttccat caacttttga gctttttcaa agccaagcat ttcaaacagg gcatcatata
     4261 acgggcgtaa gtaaggctca attttttgcc ctaaatcgcc gggtaaaaaa ccgagctttt
     4321 caccggcttc caccgccgga cgggtaagta aaatacggcg gatttcttgg cgttcaagcg
     4381 attccaccgc tgccgctacc gctaaaaagg ttttgcctgt gccagcaggc ccgataccaa
     4441 aactgatgtc gtggcttaat atatttcgta aataggcttg ttgatgttcg ctacgaggtt
     4501 taatcacccc tcgcttggtt ttaatcgcta tttcgccttg cgagccggct tgccattggt
     4561 tttgtagtag cattcggctt tcctgaatcg ccaaatgtat atcttctaag tccaattcct
     4621 taattttgcc tttaatcggg gcagtttcat tatacagttg cttaatcagc ttcacactat
     4681 tttgaaccaa ggtagcagaa tagtgattgt catcttccga ttgaatcgta aagttgaagc
     4741 ctgttcgagc aataatgaga ttaaaacttt tttcaattaa ggctaaatgc tcatcaaatg
     4801 caccacaaag cgattgcaga cgagcattat cttgcggttc taagctaaaa ataatttcgt
     4861 tcaaaattct accttctgtt aaagctgacc aaagctttca ggaatattag cggataattt
     4921 ttgccaaatt tcaccactag attggcgatt tttagccata caagcggctg ctttttcact
     4981 atcttttgca agacaagtac ataataatga acggtaaaag cttaacgtta attgacgatt
     5041 attttcatca gcaaagaaaa tttttgcgac ttgatgatac attgatttaa agctatttag
     5101 gatcaacgca tacacaggct tattggctaa aaaagtaaat tgtctgaata acttataatc
     5161 aaattcagca taagcagctg ctgtattttc taattcatct agtccatcaa agagtttagc
     5221 actttcttca ggagcaaggt aaatcgcttc tggaatataa gcctccgcca tacgagtacg
     5281 taatgacacc acattggcaa tcaccactgg aagatagctt ttatctagct taataatagt
     5341 gccaataata ttaggccccg aagtttccca aatatcattc actcttgttg gcttgccgtg
     5401 ctgaatactt aaccaacctt ctcgcgctag acgttgtagt acttcacgta aagttgttct
     5461 tgttacccca atgcgctctg ctaactctcg ctcggcagga agatcagagc cggcagggaa
     5521 atgattattc caaatactct ttacaatata ttcttccgct aacgctgccg ggctttgcgc
     5581 ttttaaaata tcactgtgac ccattaatgc cccccaagca aaaaatatgc ttatctaaaa
     5641 tctaactatt ctttatttct aatcaattat tgcgactaaa aagttcagta atac
//
LOCUS       DANLXQ010000154         5680 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.154, whole genome shotgun sequence.
ACCESSION   DANLXQ010000154 DANLXQ010000000
VERSION     DANLXQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.154"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..207
                     /locus_tag="PXD94_002208"
     CDS             <1..207
                     /locus_tag="PXD94_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287027.1"
                     /translation="SLLMNGKNDNARKEAEEHFNELQNGLLEAQDYKKDLDIKIRKLA
                     RELYNENIAQLEIEEIKEHLYKNW"
     gene            216..2282
                     /gene="glyS"
                     /locus_tag="PXD94_002209"
     CDS             216..2282
                     /gene="glyS"
                     /locus_tag="PXD94_002209"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL6287028.1"
                     /translation="MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEK
                     VEWFAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITV
                     EQAERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRP
                     VHTVTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVAD
                     FNERKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEA
                     LVYTMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFF
                     KTDLKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDNLPNSLVAC
                     SVALADKFDTLTGIFGIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSA
                     QLFGDKLTNKDVVEDVVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRA
                     VSHFRTLDSAEALAAANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEV
                     QPLIAQGDYTAVLDKLANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVA
                     DISLLQ"
     gene            complement(2387..3688)
                     /locus_tag="PXD94_002210"
     CDS             complement(2387..3688)
                     /locus_tag="PXD94_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL6287029.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(3702..4979)
                     /locus_tag="PXD94_002211"
     CDS             complement(3702..4979)
                     /locus_tag="PXD94_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287030.1"
                     /translation="MDTQKLSPAEFKQATKFDSTDFGWIIMSIGMAIGAGIVFLPVQV
                     GLMGMWVFLLSSIIGYPAMYLFQRLFINTLAESPECKDYPTVISGYLGKNWGIFLGVL
                     YFIMLVIWMFVYSTAITNDSASYLLTFGITNTLLSDNPFYGLVLICILVALSSRGEKL
                     LFKISTFMVLTKLFVVAALGLAMIGMWQLHNIGAMPPFGKLIKDAIITLPFTLTSILF
                     LQTLSPMVISYRAKEQSREVARYKALRAMNIAFAILFITVFFYAVSFTLAMGREEALK
                     AYEQNVSALAIAAQFFPGSWATYVGVALNIFAVMTAFFGVYLGFREATQGIVMNLLLR
                     KFPAEKINPNYVQKGIMLFSILLAWSAIVLNAPVLSFTSICSPIFGLVGCLIPAYLVY
                     KVPALFKYKGLALNIIIITGILLCISPFLTFLE"
     gene            complement(5008..5676)
                     /locus_tag="PXD94_002212"
     CDS             complement(5008..5676)
                     /locus_tag="PXD94_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL6287031.1"
                     /translation="MPDKLEYLHDLDKEIMLSYFSLSEAIAGLLGEFCEVVIHSLNAY
                     EKSVIKIINGHHTNRKIGSPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLVKSTTH
                     IIIGHEGKPIGLFCINLNLSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELIEETFL
                     KAVTHIEQHTTKHSKLYKKSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYKHLREF
                     KNNT"
BASE COUNT         1718 a         1124 c         1273 g         1565 t
ORIGIN      
        1 tctctactaa tgaatggcaa gaatgataat gctagaaaag aagctgaaga acattttaat
       61 gaattacaaa atgggctatt agaagctcaa gattataaaa aagatttaga catcaaaatt
      121 aggaaattag ctagagagct ttataatgaa aatatagcac aattagaaat agaagaaatc
      181 aaagaacatt tatataaaaa ttggtaattt aaacaatgac aacacaaaac ttccttgccg
      241 agatcggcac agaagagctg ccaccgaagg cactcaaaaa attagcgacc gcatttgcgg
      301 agaatgtaga aaacgaatta aaccaagcag gtctttcgtt tgagaaagtg gaatggtttg
      361 ctgctcctcg ccgtttggcg gtgaaggtgt tgagccttgc ggaaagccag ccgagtaaag
      421 aggttgagaa acgagggccg gcggtgtcgg cagcgtttga tgccgaaggc aagccgacta
      481 aggcagcgga aggctgggcg aagggctgcg ggattaccgt agagcaagct gagcgtattg
      541 ccaccgacaa aggcgaatgg ttggtgcatc gtgccgtgat tgaaggtcag ccgactaaaa
      601 atctgttggt cgatattatc gccaaatcgc tggcgaactt gccgattcca aaaacgatgc
      661 gttggggcga taaaaccgag cagttcgtcc gccctgtgca taccgtgaca cttttattcg
      721 gggcggagtt gatcgaaggt gagatcttag gcgtgaaaag cggccgtact attcgtggtc
      781 accgtttctt aggtgaacgt gagttccaaa ttgagaacgc cgatcaatat ccgcaactgt
      841 tgaaagatcg tggatctgtg gtagcagatt tcaatgagcg taaagcctta attcttgcaa
      901 aatcacagga aaaagcaacc gctttgggtg gcgttgccga tattgaagaa gatttgctgg
      961 acgaagtgac ctcgttggtg gaatacccga acgtactcac cgccaagttt gaagagcgtt
     1021 tccttgctgt gccggcggaa gccttagttt acaccatgaa aggcgaccaa aaatacttcc
     1081 cgatttattc aaaagacggc aaattgttac cgcactttat ttttgtgtcg aacatcaatc
     1141 cggaagaccc aagcaaaatc atcgagggta acgaaaaagt ggttcgccca cgtttgaccg
     1201 atgcggagtt cttcttcaaa accgacttaa aacagaagtt ggaagaccaa ctgccacgcc
     1261 ttgaaaccgt gttgttccag caacagcttg gcacattgcg tgataaaacc gctcgcattg
     1321 aagcgttggc aggtgaaatt gcgaagcaaa tcggggcaga tgaaaccaaa gcgaaacgtg
     1381 caggtctcct ctcaaaatgt gacctgatga ccaatatggt gtttgaattt actgataccc
     1441 aaggcgtgat gggtatgcac tatgcccgcc acgacggtga agatgaagaa gtggcggtgg
     1501 cgttaaacga gcagtatatg ccacgttttg ctggcgataa cttgccaaat tcattggtgg
     1561 cttgctcggt ggcgttggcg gacaaatttg acacgctaac agggattttc ggcattggtc
     1621 aagccccgaa aggcagtgcc gacccgttcg ccctacgccg tgcggcactt ggctcgttgc
     1681 ggattattgt ggagaaaaat ttaccgcttg atttaaccga tttagtggcg aaatctgctc
     1741 aattatttgg cgataaactc accaacaaag acgtggttga ggacgtggtg gactttatgc
     1801 tcggccgctt ccgtgcgtgg tatgagagcg aaggcattgc ggtggacgtg atccaagccg
     1861 ttctcgcccg ccgtccgacc aagcctgccg acttcgatgc ccgtgtgcgt gccgtgtctc
     1921 acttccgcac ccttgacagt gccgaagcct tagccgcagc gaataaacgg gtgagtaaca
     1981 ttcttgccaa agccgatatt gcaattggcg acattgatgt ttcagcttgc gtagagccag
     2041 cagaaaaagc attagccgag gcggttattg cgttgaaagc cgaagttcaa ccgctgattg
     2101 cacagggcga ctacaccgct gtgctggata aattagcgaa cctccgccaa ccggttgatg
     2161 ccttctttga cggagtaatg gtaaatgcag aagaccaaaa actgcgtcaa aaccgcctcg
     2221 ccattttaag cacgctacaa ggcttgttcc tgcaagtggc ggatatttcg ctgttgcaat
     2281 aacaataagt ttgcaataat tgcaaaattt taatgaaaat agaccgcttg cagtaattgt
     2341 gatctgcaag cggtcttttt tgcttgaaat tttaccaaag gcgggattat ttaagttttg
     2401 gtttattcac cataatctcg atgacttggc ggtctgtgta ttgcatacta cgagaggcaa
     2461 tgctgcataa gttattaatt gaacgatcaa tatcgtgttc gacaattcct tcgttgcctg
     2521 ttacttggct attatccata gacatcagaa tggatttata gcttgatgtt acgctggtgg
     2581 agactttcat cgcacaactg tttgaagcac catcacagat aataccgcta atatcgccga
     2641 tcatactact aattgccata ctgatattct caaatttccc tgtgagcagc cacgctatgc
     2701 cggcacagct tcccatagcc gcggttgtta cggcacagag tgcagataat ttcggtaact
     2761 tgctgtgaat ataaattgcc atgaggtgtg ataaaaagag tgctctaatg cgttgctcat
     2821 cagtgacatt taaatggcgg gcaacgacaa caatcggcat agttgctgta attccttgat
     2881 tacctgaacc cgaattgctc atagcaggta aacttgcacc gcccattcta gcatcagagg
     2941 cagaggtggt ttctataaca atgcgattaa gcaaatcatc actgattaga cctagaccaa
     3001 tctgtttttg caatgtcctt ccgatatgta gcccataatc ggcacgtaat ccttcttgtg
     3061 agagtgcgga atttaattgt gctgcttgct caataaagcg aattttttcc aattcgattt
     3121 gcatagaaaa attaaatata tcttttgccg aaagtgaagc aaagatctca gtagtattat
     3181 cgttattgac ggcacaaggt ttgcttaaaa tagtttgccc gtttttttca atgtaaatga
     3241 cattggtatg gtgttcagca atacgaacac atactcgatt cgcttggtga aatagtgttg
     3301 cttcagaata tagaatatgt tctgtttcga aaatagagac agacacttta tcggtattta
     3361 acatagcttt agcttgctta acctgttctg gcgtaatatt ttttaatact tctaatccac
     3421 catcaggctc ccctccgatt gctccaatag ctgccgccat aggcaatccg acggttgcag
     3481 ttcccggtac tgctactccc atcccatttt tcatcaagtt aggggaaact tttgcctcaa
     3541 tacgttctgg taaatgacct aaatattggc gtgcaatggc ggatgcaagg gcgagagaaa
     3601 taggttctgt acagcctaat gcagggacaa cttcatggtt gactgcatgt aaaatggatt
     3661 gtactaattt ttcgcagtga atattcatag ataaaacctt attactctaa aaatgtgagg
     3721 aatggagaaa tacataataa aatgccggta atgataataa tattgagtgc tagcccttta
     3781 tatttgaata atgcaggcac tttatagact aaataggccg gaatcaagca cccaactaag
     3841 ccaaaaatag gactacagat tgaggtaaag cttaaaaccg gtgcatttaa tacaatcgca
     3901 ctccaagcca gcagaatgga aaataacata atccctttct gcacataatt aggattgatt
     3961 ttttcagccg ggaattttct taataatagg ttcatcacaa tgccttgagt ggcttctcta
     4021 aaacctaaat ataccccgaa aaaggctgtc ataacggcaa aaatatttaa tgcaacacct
     4081 acataggttg cccaactacc agggaaaaat tgggctgcaa ttgctaatgc ggaaacattt
     4141 tgctcgtatg ctttgagcgc ttcttctctc cccattgcaa gtgtaaaaga gacagcgtaa
     4201 aaaaaaacgg taataaataa gatagcaaat gcaatattca tcgctcgtag ggctttatag
     4261 cgcgcaactt cacgagactg ttctttagca cggtaagaaa ttaccattgg gctgagagtt
     4321 tgcaagaaga gtatagaggt tagggtgaaa ggaagggtaa tgatggcatc ttttataagt
     4381 ttgccgaaag gaggcattgc accgatatta tggagttgcc acattccaat cattgcaagc
     4441 cctaaggcgg caacgacaaa tagcttagtt aataccataa atgtagatat tttaaacagt
     4501 agcttttcgc ctcgagaaga taaggcaact aatatacaga ttagcactaa gccataaaat
     4561 ggattatcag agagtagtgt attggtaatg ccgaaggtaa gaagatagga ggcactgtcg
     4621 tttgtaattg cggtagaata aacaaacatc caaatcacta acataataaa ataaagtaca
     4681 cctaaaaaga ttccccagtt tttgcccagg tagccactaa taacggtggg gtaatctttg
     4741 cattccggag attctgctaa agtattaatg aacagacgct gaaaaaggta catcgccggg
     4801 tatccgataa tagaagaaag tagaaaaacc cacatgccca taagtcctac ttgtaccggt
     4861 aaaaagacga tgcctgcccc gatagccata ccaatactca taatgatcca gccgaagtcg
     4921 gtagaatcaa attttgttgc ttgtttaaat tcggcaggcg agagtttttg agtatccata
     4981 gagtctcctt aaatcgtaac agtataatta agtgttattt ttgaattctc ttaaatgttt
     5041 ataaattgcg tggctggtta gggagagttt ttctgccaca ataggtactg cctcttttag
     5101 ctcaaagatg ccattttcaa acaaatagaa gataatggat ttcttataga gttttgaatg
     5161 tttggtagtg tgttgctcaa tgtgagtaac tgcttttaga aaggtttctt caataagttc
     5221 ttttgggtga gtaataaagt tttcactttt ataatgaggc ggaataggat cgtaattcat
     5281 aaaagagcta acggttttat ctaatggtgt cgataaattt aaattaatac aaaataatcc
     5341 gataggtttg ccctcatgcc cgataataat atgcgtggtg gacttaacta agttaccgtt
     5401 atttaaccgt gaaaagtaaa ccagctcagt attagattga tctttttggt aacattttag
     5461 taactgtaat cctttatctg taataggcga acctattttt cggttagtat gatgtccgtt
     5521 aatgatttta ataacggatt tttcataggc attcaatgaa tgaataacca cctcacagaa
     5581 ttctcctaat aaccctgcta ttgcttcact taatgagaaa taagacagca taatttcttt
     5641 gtctaaatca tgaagatact ctaatttatc tggcataact
//
LOCUS       DANLXQ010000155         5676 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.155, whole genome shotgun sequence.
ACCESSION   DANLXQ010000155 DANLXQ010000000
VERSION     DANLXQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.155"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            98..340
                     /locus_tag="PXD94_002213"
     CDS             98..340
                     /locus_tag="PXD94_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287032.1"
                     /translation="MTKKHYCTGWRSAPANINSCCHQHDRDYGIRGTVTRADADKRLR
                     ECMIKNGNPIRAWLFWLVVRAFGWRFYKNKEIKIEK"
     gene            330..584
                     /locus_tag="PXD94_002214"
     CDS             330..584
                     /locus_tag="PXD94_002214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287033.1"
                     /translation="MRNKDNTASKQASKQASKQASKQASKQASKQASKQASKQASKQA
                     SKQSVAQFKQAPLPFPAAVVTVVQCLRKSRKSVGVFGMRL"
     gene            786..1967
                     /locus_tag="PXD94_002215"
     CDS             786..1967
                     /locus_tag="PXD94_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDL6287034.1"
                     /translation="MSILDTYLHGVEVVEVNAGGVTISTAATSVIGVVCTGDQADAET
                     FPLNTPVLITNPLNYLEKAGSTGTLRRTLNSIGSIVKTPTVIVRVAESDDSDTLTANI
                     VGTQENGKFTGIKALLTAQSTVFVKPKLLCVPQHDNQAVATELLSVAKKLNAFAFISD
                     NGATTKEQAYTYRQNFSQREGMMIFGDWKSYNTDKKAYDTDYAVARACALQAYIDKTV
                     GWHKNISNVELDGVTGITKAVEFDINESSTEANYLNEKGITICLNHNGFRYWGSRTLA
                     TDTRWAFQQSVRTAQIIKETIGAGLAWAVDMPLTPLRVKTMLEAINNKLRSWASGDDP
                     RILGARVWVAEEITADIIKSGKFVIKYDYHWIPSLESLGLEQRVNDEYVVDLVNTLKA
                     L"
     gene            1976..2482
                     /locus_tag="PXD94_002216"
     CDS             1976..2482
                     /locus_tag="PXD94_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL6287035.1"
                     /translation="MGLPAKLKNFNFSVDGTSYLGETTEVTQPKLAMQLEDYRAGGMI
                     APVGVNMGLEKLELEFKIGGHEEDLLKLFGGSISGNAFRFNGAYQHDDDDSVDAVELV
                     CRGRIAEIDEGSSKAGDDTEHSYKASLTYYKKTVNGVDIIEIDTLNQIYIVDGKDRLA
                     EIRKAMGL"
     gene            2561..2875
                     /locus_tag="PXD94_002217"
     CDS             2561..2875
                     /locus_tag="PXD94_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL6287036.1"
                     /translation="MKKVTLKQGILRGEKRITEIEVRKPLTKQLRGTNLTSLMQLSVD
                     EWCIVLPRITTPKLDKADFATMSAADLLKLSGKALDLMSEDFDEADEEDSEEQGKGEI
                     LA"
     gene            2899..3015
                     /locus_tag="PXD94_002218"
     CDS             2899..3015
                     /locus_tag="PXD94_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL6287037.1"
                     /translation="MADIATVFHWTPNTFDEMTIVELGQWREKARLRNGVES"
     gene            3079..3426
                     /locus_tag="PXD94_002219"
     CDS             3079..3426
                     /locus_tag="PXD94_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287038.1"
                     /translation="MEIKTVTFSKVITAFIFHIITLFVFTQLNEQAQATFMFFWWFFI
                     SLRFILVVGIMCVGIEELKIQEYRQTKAYACFSFISNFALCLQFVYFEHLIAAILFLI
                     SYIIYHSQTVTIK"
     gene            3428..3865
                     /locus_tag="PXD94_002220"
     CDS             3428..3865
                     /locus_tag="PXD94_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287039.1"
                     /translation="MLQQFAMMSLGYFVFMRLTIPYQDTSREMNWSHVQNDVVGAMPK
                     SQFTGKAGETMEISAELRPEITGGKMSILALEMMAEQGSAYPLISGSTFMVLGWFVID
                     RISEQETTFFADGTPRAISFSMSLKRVDDSLLANIIDEVAGFI"
     gene            3865..5103
                     /locus_tag="PXD94_002221"
     CDS             3865..5103
                     /locus_tag="PXD94_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDL6287040.1"
                     /translation="MDFLTELTNHNHRTPAVSVTVRPKPSKDNEGEKAKDISTLITHR
                     LIQLTLTDNRGFEADQLDLELDDTDGLLALPSRGAILSVGLGWQNSPLTYKGEYTVDE
                     LTHDGPPDKVTIRARSADLRGTLTNRHERSFHRTTIGKIVKQIAEENKLKPMVGKEFE
                     NEEVKHIDQTNESSINLLQRLAEQFDAIATVKNGNLIFIKAGNATTASGKLLPLFRIT
                     RSSGDSHSFSIAEGDNYKAVKAYWHNTQTGKRGEVTWDENSQVKKVTKPTMRKKTKVK
                     RGADGKPIKGKDGKSIKETVFVKGKGRQVNAVVQSKPIESDSEAIKTLPHTYATAQSA
                     INACKRNFEKLQRGVATFSLTLAEGNAELIPELPVQVSGFKAEIDSNEWIISQVTHSL
                     NKGGGFTTALEMELKPKSED"
     gene            complement(5286..>5676)
                     /locus_tag="PXD94_002222"
     CDS             complement(5286..>5676)
                     /locus_tag="PXD94_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287041.1"
                     /translation="KYNKADEAMHIIANNIIRKKTAVANAILALNEVDSRKSSGVQLC
                     DLLLGAILSSYQNDITSERKKELTKWIALHLGWDKLTYDTLQEEKKFNIWYFYDPTKG
                     PRIVRTRETKLKYPLLKKKEQTFQPAR"
BASE COUNT         1716 a         1188 c         1292 g         1480 t
ORIGIN      
        1 aggtgacatt gttgatttgc agtataaaac aacgcatgag ttgacattac tgtcagactc
       61 atttttatct caaatttatg tgactcagga gttttaagtg actaaaaaac actactgcac
      121 aggctggcgt agcgcaccag ccaatatcaa cagttgctgt catcaacatg accgagacta
      181 cggcattcgt ggcacagtaa cacgagcaga tgctgataaa cgcttacgtg agtgtatgat
      241 taaaaatggc aatccaatta gggcttggtt attttggcta gttgtgagag cttttggttg
      301 gcgtttttat aaaaataagg agattaagat tgagaaataa agataatact gcaagcaagc
      361 aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc
      421 aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa agtgtagcgc
      481 aatttaaaca agctccgcta ccttttccag cagccgttgt taccgtggtg caatgcttgc
      541 ggaaaagccg aaaaagcgtt ggcgtttttg gtatgcgttt gtgaatggct tgttttttga
      601 taaaaagcat tgctatgcag cctataaagc ggaggtgtat cgaaaacagt atccaccgga
      661 gtttaccgaa atcacttaat cctgttaaac cccatttcac agcccgaacc gctcgcaacc
      721 cgagcggtta ttttttaaag tttaccccaa cattcaaccc taacctttcc caattaagga
      781 atactatgtc aattttagat acctatctac acggtgttga ggtggttgag gtcaatgcag
      841 gtggtgtcac catttccacc gctgctactt cggttatcgg agtagtttgc accggagacc
      901 aagccgatgc cgagactttt ccgctgaata cgcccgtttt aattactaac ccgttaaatt
      961 accttgaaaa agcaggtagc accggcacgt tacgccgcac acttaattcg attggctcaa
     1021 tcgttaaaac gccaacggtg atcgtgcgag ttgccgagtc ggacgatagc gacaccttaa
     1081 ctgcaaatat tgtcggcacg caggaaaacg gcaaatttac cggtattaag gcgttattaa
     1141 ccgctcaatc aaccgtgttt gttaagccga aattgctttg tgtaccacag cacgataatc
     1201 aagccgttgc gaccgaacta ttaagcgtgg ctaaaaagct gaatgcgttt gcgtttattt
     1261 cagacaacgg tgcaaccacc aaagaacagg catataccta tcgccagaat ttctctcagc
     1321 gtgaaggaat gatgatcttc ggtgactgga aatcttataa cactgataaa aaagcctatg
     1381 ataccgatta tgcagtggcc cgagcttgtg cgttgcaagc ctatatcgac aaaactgtcg
     1441 gctggcataa aaacatctca aacgtagagc tagacggtgt aaccggtatc actaaagcgg
     1501 tagagtttga tattaacgag agctcaaccg aggcaaacta cctgaacgaa aaaggcatta
     1561 ccatttgctt aaaccataac ggtttccgct attggggttc ccgcactctt gccaccgata
     1621 cccgctgggc gtttcagcag tcggtgcgga cggctcaaat cattaaagag acaatcggag
     1681 caggcttagc ttgggcggtg gatatgccac tcacaccgtt gcgtgtgaaa acaatgctag
     1741 aggcgattaa caacaagcta cgctcttggg catcgggtga tgaccctcgc attttaggtg
     1801 ctcgtgtgtg ggtagctgaa gagatcaccg cagatattat caaatcaggc aaatttgtta
     1861 ttaaatacga ttaccattgg attccgtccc tcgaaagcct aggcttagag caacgggtca
     1921 atgatgaata tgtagtggat ttagtcaata cacttaaagc gttatagagg taaaaatggg
     1981 attaccggca aaacttaaga attttaattt ttcggtggac ggcacaagct atttgggcga
     2041 aaccaccgaa gtcacacagc cgaaactggc tatgcaactt gaagactacc gagcaggcgg
     2101 aatgattgcc cctgtgggtg tcaatatggg tttagagaaa ctggagcttg agttcaaaat
     2161 cggcggacac gaagaggatt tgctcaagct atttggtggc tcaatcagtg gtaatgcgtt
     2221 ccgtttcaac ggtgcgtacc agcacgacga tgacgattca gtcgatgcgg tagagttagt
     2281 ctgccgtggg cgaatcgctg aaattgacga aggctcaagc aaagccggtg atgacaccga
     2341 gcatagctat aaagcctcgc taacctatta caaaaaaacg gtgaacggtg tcgatattat
     2401 cgaaatcgac accctcaacc aaatttacat tgtcgatggc aaagaccgct tagcggaaat
     2461 tcgtaaggca atgggtttat agattttccc ctaaattttc ccaaagcccc ttcccctttt
     2521 ttcaaggggc ttttaatgat aaataattaa ggattaaaca atgaaaaaag taacattaaa
     2581 acaaggcatt ttgcgtggtg aaaaacgtat taccgagatt gaggtgcgta agccattaac
     2641 caaacaactg cgtggcacaa atcttacctc attaatgcag ttaagcgttg atgagtggtg
     2701 tattgtactg ccacgcatta ccacgccaaa attagacaaa gccgattttg ccacaatgtc
     2761 ggcagcggat ttgctaaaac tctccggcaa agccttagat ttaatgagcg aagacttcga
     2821 tgaggcagac gaagaggaca gcgaagaaca gggaaagggc gagattttag cctaattcca
     2881 cagtttgttg atgacgctat tgctgatatt gctaccgtgt ttcattggac acccaacacc
     2941 tttgatgaaa tgacgattgt cgagctaggg caatggcgag aaaaggcccg cttacgaaat
     3001 ggggtggaaa gttaaaacaa gcggtcgttt ttcgcaaaaa atttgcaaaa ctcgaccgct
     3061 tgctataagg attttaaaat ggaaattaaa acagttacat tcagtaaagt gattacagca
     3121 ttcatttttc atatcattac gttatttgtt tttactcaac ttaatgagca agctcaagct
     3181 acctttatgt tcttttggtg gttcttcatt tctctgcgat tcatccttgt agttggaata
     3241 atgtgtgttg gtattgaaga acttaaaatt caggaatacc gacaaacaaa agcttatgct
     3301 tgttttagtt tcatatccaa ttttgcgtta tgcttgcagt ttgtttactt tgagcatctt
     3361 attgcggcaa ttttattttt gatttcttac atcatatatc atagccaaac tgtaacgatt
     3421 aaataatatg ctccaacaat tcgcaatgat gtcgctaggc tattttgtgt ttatgcgatt
     3481 gacaatccct tatcaagaca ccagccgaga aatgaactgg tcgcacgttc aaaatgatgt
     3541 ggttggggca atgccaaaaa gccaatttac cggcaaggca ggggagacaa tggagatttc
     3601 agccgagcta cgccctgaaa ttaccggggg caaaatgtca atccttgccc ttgagatgat
     3661 ggcagagcaa ggctcggctt atccgttgat tagtggttca acctttatgg tgctaggctg
     3721 gtttgtgatt gatcgaatca gcgagcagga aaccaccttt tttgcagacg gcacgccccg
     3781 ggcaattagc ttttcgatgt cgcttaaacg ggttgatgat agtctgctcg ccaatattat
     3841 tgatgaggtc gcagggttta tttaatggat tttttaacag agctaaccaa tcataaccac
     3901 cgcacaccgg ctgtttcggt gacggttcgc ccaaagccaa gcaaagataa tgagggcgaa
     3961 aaagccaaag atatttccac cctgatcacg caccgcttaa ttcaactcac cttaaccgac
     4021 aaccgaggct ttgaggcaga ccagctggat ttagagctgg acgacactga tggcttactc
     4081 gctttgccaa gtcgaggagc gattttatcg gtcgggttag gctggcaaaa cagccccctg
     4141 acctacaagg gcgaatacac ggtggacgag ctaacccacg atggcccacc tgataaagtc
     4201 acgattcggg caagaagtgc tgatttgcga ggcacgctga ccaatcgcca cgagcggagc
     4261 tttcaccgca ccaccattgg taaaattgtg aaacagatcg ccgaagagaa caaactcaag
     4321 ccgatggtgg gcaaggagtt tgaaaacgaa gaggtgaaac acattgacca aacaaacgaa
     4381 agctctatta atctcttgca acgcctcgca gaacaatttg atgccattgc taccgtgaaa
     4441 aacggcaatt tgattttcat taaagccgga aacgccacaa ccgcaagcgg taaactgtta
     4501 cccctgtttc gcattacccg cagttcgggg gattcccata gcttttcgat tgccgaaggg
     4561 gataactaca aagcggttaa agcctattgg cacaacacgc aaaccggcaa gcgaggcgag
     4621 gtgacgtggg acgaaaacag ccaagttaaa aaagtgacta agcccacaat gcggaaaaag
     4681 actaaagtaa aacgtggggc agacggcaag ccgataaaag gcaaagacgg caagagcatt
     4741 aaagagaccg tctttgtaaa aggcaaaggc aggcaagtta atgcagtggt gcaaagtaaa
     4801 ccgattgaaa gtgatagcga ggcaattaaa accctacccc atacttacgc taccgcacaa
     4861 tcagcgatta atgcttgtaa acggaatttt gaaaaactac aacgaggtgt ggcaactttt
     4921 agcctaactc ttgccgaggg caatgcggag cttatccccg aattaccggt tcaggtttca
     4981 ggctttaagg cggagattga tagcaacgag tggataattt cgcaggttac gcacagttta
     5041 aataaaggtg gtgggtttac gacagcgtta gagatggagt tgaagccgaa gtctgaagac
     5101 taacgctatt tttataaaat cactataaaa aagccttgca tctctgatct tgaacaagta
     5161 atattaatca aagttacaga tttatttaca taaaacacaa agaatcatag gggcggctac
     5221 tttgtgtgat tccgcccagt gaggaaattg ggcgttgttt tacacatata agtccttgag
     5281 gacactcacc gtgcaggctg gaaggtctgc tctttctttt ttaatagtgg gtatttaagt
     5341 tttgtttctc tcgttcttac tattctaggt ccttttgtag ggtcgtaaaa ataccaaata
     5401 ttaaatttct tttcctcctg taaagtatca taagttaatt tatcccaacc taggtgcaaa
     5461 gcaatccatt ttgttagttc ttttttacgt tccgatgtaa tatcattttg atagctactt
     5521 aatattgctc ctaataataa atcgcatagt tgaacgccag atgatttacg agaatctact
     5581 tcgtttaatg ctagaatagc atttgctacg gcagtctttt tacgaattat attattagcg
     5641 ataatgtgca ttgcctcatc agctttattg tattta
//
LOCUS       DANLXQ010000156         5635 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.156, whole genome shotgun sequence.
ACCESSION   DANLXQ010000156 DANLXQ010000000
VERSION     DANLXQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.156"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            62..493
                     /locus_tag="PXD94_002223"
     CDS             62..493
                     /locus_tag="PXD94_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL6287042.1"
                     /translation="MFLSGFLSATLLPGNSEIIFTTLASQHLITYGSLSSFSLFNLLL
                     VATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAKSEKYGVLVLLFSWLPIVGDIF
                     CGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVLPHIL"
     gene            complement(547..1416)
                     /gene="htpX"
                     /locus_tag="PXD94_002224"
     CDS             complement(547..1416)
                     /gene="htpX"
                     /locus_tag="PXD94_002224"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDL6287043.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEVAHIANGDMVTMTLL
                     QGVLNTFVIFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLATMIAMWF
                     SRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGKELFMSH
                     PPLEKRIEALRNL"
     gene            complement(1504..2586)
                     /gene="dinB"
                     /locus_tag="PXD94_002225"
     CDS             complement(1504..2586)
                     /gene="dinB"
                     /locus_tag="PXD94_002225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL6287044.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKSVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKITS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVEPYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
     gene            2709..3620
                     /locus_tag="PXD94_002226"
     CDS             2709..3620
                     /locus_tag="PXD94_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL6287045.1"
                     /translation="MLYGLAIVLIPLFLGYLITLKNHRHMAIVNRVVSLCLYLILLVM
                     GISLGQIDNILTELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSR
                     LHLLIDSFKLIGITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLR
                     EVFFNKRGIYTSIVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAW
                     GPIYGSIAFFNDISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQKSGGIQI
                     VPLAISFGFIVNLAAPLLLAVFIGLEG"
     gene            3726..5492
                     /gene="aspS"
                     /locus_tag="PXD94_002227"
     CDS             3726..5492
                     /gene="aspS"
                     /locus_tag="PXD94_002227"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL6287046.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKETLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNEEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
BASE COUNT         1584 a         1227 c         1142 g         1682 t
ORIGIN      
        1 gacagaatat ctcacttcct ttttcagttt attcttttcc gaacaaaatc aactctttac
       61 tatgtttctg agtggatttc taagcgctac cctcttaccc ggaaattctg aaattatctt
      121 cactacctta gcaagccaac atctcatcac ttatggctct ttatcctcat tttcactgtt
      181 caatttactc ttggttgcaa ccttaggtaa tagtttaggt agcataacca cttatgcgat
      241 agggctatta ctccctcgcc cacagaatac taccaatcgt tattcacaat gggcattagc
      301 caaaagtgaa aaatatggcg tgttagtctt gctttttagt tggttaccga ttgtaggcga
      361 tattttctgt ggcattgccg gctggctacg gtttagcctt tggcaaagca ttctctttat
      421 tgtgattggg aaattgttac gttatctgtt tttgctggca actctctatt cggtgttacc
      481 gcatatttta tagaaaacaa aagcggctgt tttgcaaaaa attttacaaa aacaaccgct
      541 tgtgctttat aagtttctta acgcttcaat acgtttttct aacggagggt ggctcataaa
      601 caactcttta ccacggctac cgttaatcat aaaggcagca agagagcctt ccatttcttg
      661 cggttcgtga acacgttgca agcgttgtaa tgccgcaatc attttctctt taccgactaa
      721 ttgagcagag cctgcatctg cacgaaattc acgttggcga gagaaccaca ttgcaatcat
      781 tgttgctaac acaccaaaca ccatctgtaa cacaatatct actacccaga acaccagcgt
      841 attggtgctt gaattgccat tttcgtcacg tgaacttgcc gccattgtcg aaataatacg
      901 agctaagaaa atgacgaaag tatttaacac gccttgtaat aaagtcattg taaccatatc
      961 gccattcgca atgtgcgcca cttcgtgtgc tactaccgct tcagcttcgt ctttagtcat
     1021 tgaattcagc aagccggtgc tgactgcaac taaagaatta cttttcgttg ctcctgtagc
     1081 gaatgcattt acatcagccg aatggtaaat cgcaatgtcc ggcattggga tattcgcttg
     1141 ctgagcctga cgttgtacag tgttgaataa ccattgctcc gcttcgttac gaggctgtgt
     1201 aatcacctct gccccaaccg aacgcaatgc cattgatttc gacataaata gcgaaattaa
     1261 agacccactg aaaccgaaga ctaacgaaag caccaaaata ccggcagtgc tttgcccttg
     1321 aatgccggtc actttaaaaa taaggtttaa tacgatccct aaaacaaagg taatcgctaa
     1381 gttagttaat aaaaatagaa tgacacgttt tgccataatg ttttcaatct ccaaaaatat
     1441 agatgcaatg attgcacgaa taagataatg actttagcct taatttcaag tcattattta
     1501 caattattcc cacaaattta actgtttatt cacttttttc tcgggaaagt gaacacttag
     1561 accgattaat cgcacctttc gttcctttct tcgggtaaaa atttgctgta gcaactcgcc
     1621 aaatcgctca tacgcaaagc catcggtggt tcgttcaagc gtggtttggc taaaatcatc
     1681 aaacttgagt ttaatgccta actttttaaa ctcagttaat ggcgtatcgc cccagtttcg
     1741 ttgcaaccga aaaaccagtt tgtcgaataa atctgccaca atcttttccg cttgctcaag
     1801 ggttgaaata tcgtgcaaca gcgtattttc caccgccagc gattttcttt cacgataagg
     1861 ctctaccgct cgttcatcaa taccgtggct aaaatcccaa agccgttgcc cgaatttgcc
     1921 aaacgctcgg taaataaccg ctttttctgc ttgctgaata tcaccacagg tttcaaagcc
     1981 aagctgtttt aatttttcat tggttacttt ccccacaccc ggaatttttg caagcggtag
     2041 atttttcaca aaattttgca catcatcagg cgtaatcaca cattgtccgt taggtttgtt
     2101 ttgatcggag gtaatttttg ctaaaaattt aagcggagca actcctgccg aggcagttaa
     2161 atgcaattca ttgaaaatat cattgcggat tgcttgagca atccaagtgg cggagccaaa
     2221 atagcgttcg cattcggtta catctaaata ggcttcatca agcgacaagg cttcaatcac
     2281 atccgtatag cgatgaaaaa tttcgtgaat ttgagcggaa accgatttat acaacgccat
     2341 attcaccggc aataaaatta aattcgggca tttcttcaac gcagttgccg ttggcattgc
     2401 actgtgtaag ccgaattttc gagcctcgta attacaggtt gtcagcacac ctcgttgatt
     2461 agcattgccg cccaccgcca ccggcttacc gaccaacgcc gggttttccc gcatttcgac
     2521 cgctgcataa aaacaatcca tatcaatatg gataattttt cgcttatgtt tagcttgaat
     2581 cgacatttgc aatattttcc ccaaaaacga ccgcttgcaa cacaaataac tgtatataaa
     2641 aacagtttta ttttatgccc ttattgttag aatttcaagt ttaaaaatag cgataaaaag
     2701 gataaaaaat gctctacggt ttagccattg tgctgattcc actttttctc ggttacttaa
     2761 taaccctcaa aaaccaccgc catatggcaa tagtaaacag agtggtcagc ctctgccttt
     2821 acttgatttt attagtgatg gggatttctc tcggacaaat tgataacatt ctcaccgaac
     2881 tgccacagat tggcggtacg gcgtttagtt tagtggtgat tttactcctt tcaaacatta
     2941 tcggcttggt gatttacgac aaattacaac cgatgaaaag agacaaagtc aatcaagatc
     3001 aacttccctc ccgcctgcat ttgttgattg attcctttaa attaatcggc attaccttat
     3061 taggcgggat catcggctat ttcaccaaag gcgtggtgga tttccctctg cacgccagca
     3121 cctatgtgtt agaagtgatg attttcgggg tggggattca actgcgaaac agcggtattc
     3181 ccctgcgtga agtgttcttt aacaagcggg ggatttatac ctcgattgta atggtggcaa
     3241 gttccctgat tggtggcgtg atttcggcat tcaccttaga tttatccctt gctaaaggct
     3301 taacctttgc ctctgcattc ggttggtatt ccctttcaag cgtattggta aacgatgctt
     3361 ggggcccgat ttacggcagt attgcctttt ttaacgacat ctcgcgtgaa attttatgcc
     3421 tgtttttagt accactgttt atgcgtcaat ttccgtccac cgcagtcggc attggcggag
     3481 cgacttcatt agattgtatg ctccctatta ttcaaaaatc gggcggtatt caaattgtgc
     3541 cgctggcaat cagcttcggt tttattgtta atttagccgc tccattgtta ttagccgttt
     3601 ttattgggct ggaaggataa atcaccgctt gtttgctttt atttatccat cttcgctgaa
     3661 ttttccgcta taatccttaa gttttccccg aaacttggga tatttatttt caaaaggata
     3721 attttatgat gcgttctcac tactgcggtg cattaaaccg ctcacacctt ggacaaacag
     3781 ttaccttaag cggttgggtt catcgtgttc gtaaccttgg tcgttttatt tttatgcaaa
     3841 ttcgtgatcg tgaaggcatt gtgcaggtgt ttttcgatga gaaagatgaa gcacttttca
     3901 agcaagcatc ggccttacgt tctgaggcgt gcgtgcaaat tcaaggtgaa gtgattgccc
     3961 gtgatgaatc gcaaatcaat aaagagatgg caaccggtga aattgaagtg ttagtaaaaa
     4021 atttagtggt gtataacaac gctgaagtat tgccgttaga cttcaatcaa aacaataccg
     4081 aagaacagcg tttaaaatac cgctatttgg atttacgccg ccctgaaatg gcagaaaagc
     4141 tcaaaacccg tgctaaaatc accagttttg tccgccgttt tatggacgat aacggtttct
     4201 tagacattga aaccccgatg ctgaccaaag ctactcctga aggtgcgaga gattacctcg
     4261 tgccaagccg tgtgcataaa ggcaaattct atgctctgcc acaatcacct cagctattta
     4321 aacagttgct aatgatgtcg ggcttcgacc gttattacca aatcgtgaaa tgtttccgtg
     4381 atgaagattt gcgtgccgac cgccagccgg aatttaccca aatagacgtg gaaaccagct
     4441 ttatgactgc cgaagaagtg cgtgcattaa tggaagcgat gatccacggc ttatggaaag
     4501 agaccttagg cgttgatctc ggtaaattcc caatgatgac gtggaatgaa gcaatgaccc
     4561 gtttcggttc agacaaaccg gatttgcgta acccactgga aatggttgat gtggcagata
     4621 ttttaaaatc ggttgatttc aaagtattta gcggtccggc gaatgatccg aaagggcgtg
     4681 tagtggcact tcgtgtgcca aacggtgcag ctctaacccg taaaaatatt gatgactaca
     4741 cccaatttgt cggcatttat ggggcaaaag gcttggcttg gttaaaagtg aatgatatga
     4801 acacaggttt agaaggcatt caaagcccgg ttgcaaaatt cttaaatgaa gaagtgctaa
     4861 aagccttagt taaacgctta aacttgcaaa aagacgacat tgtattcttc ggtgcggata
     4921 actggcacgt tgcgactaac gccatcggtg cgttacgctt aaaagtgggg aacgatttag
     4981 gcttaaccga taccacagcg tggaaaccgt tatgggtgat tgatttccca atgtttgaac
     5041 gtgatgatga aggcaactta tcggcaatgc accacccgtt cacttcaccg aaagaattat
     5101 cgccggaaga attagtgcaa gatccggaaa atgcggtggc aaacgcttac gatatggtaa
     5161 ttaacggcta cgaagtgggc ggtggctcgg tacgtatttt cagccagcag atgcaacaaa
     5221 ccgtgttcag cattttaggc attaacgaag aagagcaaaa agagaaattc ggtttcttat
     5281 tagatgcgtt aaaattcggt actccgccac acgcaggctt agccttcggt ttagaccgct
     5341 taacgatgtt gattacagga actgataaca ttcgtgatgt catcgctttc ccgaaaacca
     5401 ccgctgcagc gtgtttaatg acagaagcac caagctatgc gaatccgaaa gcgttggaag
     5461 agttagcaat ccgtacaatt ccacaagagt aatcggtagg ggcgaaacat ttttcgccct
     5521 ttttatttaa caagcggtcg aaaaagtgaa aaactttgca aaagtaataa tcgcaaaatt
     5581 attctctaaa taacataata aagactcttt tctcttctct atgttactac taaat
//
LOCUS       DANLXQ010000157         5544 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.157, whole genome shotgun sequence.
ACCESSION   DANLXQ010000157 DANLXQ010000000
VERSION     DANLXQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.157"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(107..622)
                     /locus_tag="PXD94_002228"
     CDS             complement(107..622)
                     /locus_tag="PXD94_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL6287047.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GARREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            777..3605
                     /gene="uvrA"
                     /locus_tag="PXD94_002229"
     CDS             777..3605
                     /gene="uvrA"
                     /locus_tag="PXD94_002229"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL6287048.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRLMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNSV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFPLAQNALNRAENSDVAPYESIDGLSHFD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIREIFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPMPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXD94_002230"
     CDS             complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXD94_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL6287049.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
BASE COUNT         1601 a         1152 c         1301 g         1490 t
ORIGIN      
        1 gttccctatt attttgagta aggtaggggc gctttatatg agtagctttt tatttcatcg
       61 aaatgcggtg tatggaaccc atgagctact taagtcaata ctttgattag aacggaatat
      121 catcatcaaa attgttcatt gccggctcag gctgtggtgc cggtttagat tgcggtgcag
      181 cactttgggt ttggttgtaa ccataattgc tgttgccttg attgtagcta ttacccgcag
      241 gagcagaatt tccccaaccg ccttgctgac cgcccatatc attaccttga cggctgccga
      301 gcatttgtag cacatcgcct tgaatttcgg tggtgtaacg atcttgcccg ttttggtctt
      361 gccatttacg ggtttttaaa cgtccttcta cgtaaacttg agagccttta cgtaaatatt
      421 gcccgcatac ttctgcttga cggcggtaga atacaatacg atgccactcg gttacttcac
      481 ggcgtgcacc ggtgttttta tccgtccagc tttctgaggt tgccacacta atatttgcaa
      541 ccgcttcgcc atttggcatt gtacgcattt cagggtcgtt acctaaatta ccgacaataa
      601 ttactttatt gataccagcc ataaaatcct ctgtttgata aattaaacta aaatttgcta
      661 agtattctac ctgaaaatcc aaaagatgaa caccaaaaaa ctgatatttt gtacagtttt
      721 attaaaatat gcgataatga tcgaaaattg agaaaatagc actggacatt aaaaatatgc
      781 aacatatcga tattcgtgga gcaagaacac acaacttaaa aaacatcaat ttaaccttgc
      841 cgagagataa attcattgta attaccgggc tttccggctc gggcaaatcg tctctggcgt
      901 ttgatacgct ctatgcggaa ggacaacgcc gttatgtaga atcactgtct gcatacgccc
      961 gccagtttct ctccttaatg gaaaagcctg atgtggatca tattgaaggg ctttcccctg
     1021 cgatttcgat tgagcaaaaa tcaacctctc acaacccacg ttcgaccgta ggaacggtaa
     1081 cggaaattca cgactattta cgtttattgt ttgctcgagt gggcgagcct cgttgccctg
     1141 accacgatgt gccattggct gctcaaacta tttcgcagat ggtggatcga gtattggaag
     1201 aaccggaagg aaaacggttg atgctacttg cccctgtggt gaaagagcgt aaaggcgaac
     1261 acgtaaaatt attggaaaat ttgaccgcta gtggctattt gcgggctaga attgacggtg
     1321 aaatttgcga tttatccgac ccaccggcat tagaattaca gaaaaaacac accattgaag
     1381 tggtggtgga tcgctttaaa gtaagggctg atctagccac ccgtttggcg gaatcttttg
     1441 aaacagcgtt agatttatcc ggctctaccg caattattgc agatatggac gacccaaatg
     1501 ccgaagaaat tgtgttttcg tccagctttg cttgtccgca ttgtggttac tctttacacg
     1561 aattagaacc acgccttttc tcgtttaaca acccggcagg tgcttgcccg acttgtgatg
     1621 gtttgggcgt tgagcagttt tttgatgcca aaaaagtagt gcaaaatgaa gaggtttcat
     1681 tagccgccgg tgcgatcaaa ggctgggatc gccgcagttt ctactatttt ggcttattaa
     1741 aagccgttgc cgagcattat ggctttgatt tagaaacccc attcgggcag ctaccgaaaa
     1801 aatatcaaaa cattatttta aacggcacta aagaagagat tgagtttgtt tatgtcaatg
     1861 accgaggcga taaggtcaaa cgtaaacacg cttttgaagg ggtgctgaat aatatggctc
     1921 gccgctataa agaaaccgaa tcaaactcgg tgcgtgaaga gttggcgaag tatattaata
     1981 atcgtccttg tatggattgc gaaggctcac ggttacgccg tgaagcccgt tatgtattct
     2041 tagataaaac caatttgccg atggtttcgg aaaaaagtat cggcgaggca ttaacctttt
     2101 ttgaagagct gaaattagcc ggtcagaaag aaaaaattgc tgagaaaatc ctcaaagaaa
     2161 ttcgtgaacg gctacagttt ttagtgaatg tcggcttgaa ttatctttct ctttcccgct
     2221 cggcagaaac cctttcaggt ggcgaggctc agcggattcg ccttgccagc caaattggtg
     2281 cgggtttagt aggggtaatg tatgtgctgg atgagccttc catcggcttg caccaacgag
     2341 acaatgaacg cctactgaat actttggtgc atttacgcaa tttaggcaat accgtgattg
     2401 tggttgaaca cgatgaagat gcgatccgag ccgcagatca tatcgtggat ataggcccgg
     2461 gtgccggtgt acacggtggg caagttatcg cacaaggtac ggcagctgaa attatgcaaa
     2521 ttgaggaatc tattaccgga caattcctct caggcaagca aaaaattgag attccggctc
     2581 agcgtgtgcc ttatgagcca actaaattgc tcaccttatc gggagcaagt ggtaacaact
     2641 tgaaaaaagt gaaattagag attccggttg gtttatttac ttgtatcacg ggtgtatcag
     2701 gttcgggcaa atcaacttta gttaatgaca cactattccc tctggcacag aatgcgttaa
     2761 accgagcgga aaacagcgat gttgctcctt atgaatcgat tgacggctta tcgcattttg
     2821 acaaggtaat tgatattgac caaagcccga ttgggagaac tcctcgctca aatccggcaa
     2881 cttacaccgg attatttacg ccaattcgtg agatttttgc cggcacgcaa gaagctcgag
     2941 ccagagggta taacgtaggg cggtttagct ttaatgtgcg gggcggacgt tgcgaagcct
     3001 gtcaaggtga cggcgtgatt aaagttgaaa tgcacttctt gcctgatgtg tatgtgcctt
     3061 gcgatcactg taaaggcaaa cgctataacc gtgaaacctt ggaaattcgc tataaaggca
     3121 aaactattca ccaagtgtta gagatgacgg tggaagaggc gagagaattt tttgccccta
     3181 tgccgatgat tgcccgcaag ttgcaaacct tgatggacgt ggggttatct tatatccgtt
     3241 tagggcaatc ttctactacg ctctcaggcg gtgaagccca acgggtgaaa ttggcgaccg
     3301 agctttccaa acgagacacc ggcaaaacgc tctatatttt agatgaaccg accaccggct
     3361 tgcattttgc cgatattaaa cagttactcg gcgtgctaca tcgcctgcgt gatcaaggca
     3421 atacgattgt ggtgatcgaa cataatttag atgtgattaa aaccgcagac tggattgtcg
     3481 atcttggccc tgaaggtggc agtggcggcg gtgaaattat tgctaccggc acaccggaag
     3541 aagtggcaaa agacaaacgt tctcacaccg cacgcttctt aaaagagatt ttggctaaag
     3601 gctaacgttc aacacaagcg gtcatatttt cggaaaatgt tgcaatttgc aaaaagttgg
     3661 aaaatatgac cgcttgtttt atatccggag ttattcctag ttagcctgtc tgattttgtc
     3721 gaaggcagat tcaagatcgc tactgaattg aatttgcggg atttcgttta ccgttttttg
     3781 tgagcggact agcattctca tcggctgaaa ttgcacatta cagagatgaa gttgttgatg
     3841 tggcaacata tgctgtacaa agcgggttag ggcgtgaatg ccaccagcat ctaatactgt
     3901 aacggcatcg cattgcagca caatatgttt aatttggtga tcggtatgta cggttttatt
     3961 gtgcagatcg gcaaagagat tatcggcggc agcaaaaaat aacggaccgc tgatccgata
     4021 aaccaacaca tcgttcaaat cggttgggtg agccatttca aaggatttag tcatttctgc
     4081 aatagtacgg ataaatagca ggcttgccag taacacccct accgtaatgg caattaccat
     4141 atcaaataat accgtgaggg taatgcaagt cagtaaaacc gcaatttcat ttttacctga
     4201 acggcgggta agttgaatga tttgtggaat atctgccata ttccaagcta ctaccaataa
     4261 tagtgcagcc attgaggaaa gaggcaaata ggaaagtgct ttagccaaaa agagtagggc
     4321 aaataaaacc aacaaggcgt gaaccacact agaaatgggg gaaactgccc cggaacgaac
     4381 attggcagcc gatctcgcaa tcgctgctgt ggcggtaatt ccccctaaaa atggcgaggc
     4441 aatattacct aagccttggg ctaatagttc gttgttagag tggtgcttgg tatctgtcat
     4501 attatcgcac actaccgcac ataataacga ttcgatagcc ccaagcattg ccattgaaaa
     4561 ggcagccggt aataaggctt gaatggtggt taagttccaa tctatccgat tcccctctgc
     4621 attcgggaga ttccaaggta acacaaattc aggtaatacg cttgggatac catacccagt
     4681 ggtgccgtca gagagcgtgt agtggaatgc tgagccgata gtggcaacgt tttgtccgaa
     4741 atgaatcagc agtaatgata acagcgtgcc gataaagacg gcgggtaaat gcccgggaat
     4801 aggcaaccgt agcttgtgcc aacgagttaa aaccaacaat gttacgacac ccactaaggt
     4861 gtcagcccaa tttatggttg gcaatgctga aaaaatagcc tctagcttgc caagatagtg
     4921 ttcaggcatt ttttcaatgc ttaagcccag aaaatctttg atttgcaagg tggcgatcac
     4981 aataccgatg ccacaggtaa aacccagtgt gacaggtaga ggaatgtatt caattaagcg
     5041 tcctaatcgg agtaacgcca tcaatactaa aattcctccg gaaagcattg ttgccatgag
     5101 taatccactt aagccaaact gctgcgtgac ggggtataga atcaccacaa aagcggctgt
     5161 tgggcctgaa atattaaatc ttgaaccgcc tgttagggca atcacaatcc cggcaacaat
     5221 cgccgtatat aaaccgtgct gtggtggcac accgctggca atcgccaacg ccatagataa
     5281 cggaatagca ataacgccaa cggttaagcc tgaaataata tctctgatta aatgttgttt
     5341 accataacct gcattgagag catcttttaa tgcactaaaa ggcttgacgg caagaaaagc
     5401 atttttagtt aaaaaccatt tatttaacat agaatgttcg agtgaagaga atgtttcgct
     5461 tattttactg caatgaataa aattaaattt gagctaacgc aagaaaaagc aggaaagacg
     5521 aaatatgttg gagacaagcg gttg
//
LOCUS       DANLXQ010000158         5475 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.158, whole genome shotgun sequence.
ACCESSION   DANLXQ010000158 DANLXQ010000000
VERSION     DANLXQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.158"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            162..1796
                     /locus_tag="PXD94_002231"
     CDS             162..1796
                     /locus_tag="PXD94_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL6287050.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     FEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIDGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVQKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
     gene            1850..2122
                     /locus_tag="PXD94_002232"
     CDS             1850..2122
                     /locus_tag="PXD94_002232"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL6287051.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(2182..4794)
                     /gene="pepN"
                     /locus_tag="PXD94_002233"
     CDS             complement(2182..4794)
                     /gene="pepN"
                     /locus_tag="PXD94_002233"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL6287052.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAATKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(4944..5462)
                     /gene="hutZ"
                     /locus_tag="PXD94_002234"
     CDS             complement(4944..5462)
                     /gene="hutZ"
                     /locus_tag="PXD94_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL6287053.1"
                     /translation="MTTNRQEVLQNRLGPEIEELKQQIKTIMLATIDKDGVPNVSYAP
                     FVINNGEYQVLITTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIE
                     RTDEEWNNSIAALKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLI
                     NVVHLDQGHQTA"
BASE COUNT         1611 a         1092 c         1174 g         1598 t
ORIGIN      
        1 ttcttcggga tttttttatg caatttttgc attgaaaggt gatatctatc acatttttta
       61 aaaaaattcc ttgcaatcaa aataataatg cctagactta aaaaaattaa ttagaacaaa
      121 tcggctggtt gcaagccaat attcatcaat ggagatcaaa tatgcagtca aaattctcgc
      181 tttccttatt aacaacctca ttagtgttag gcttatcggc aaatgtgttt gcagccaaag
      241 tgcctgaagg aacaacgctt gcagaaaaac aagaaattac gattaataat ggttttgagc
      301 catcaagttt tgatccacat aaaattgagg gcgtgccgga atcgcaagtt gcttatcagt
      361 tatttgaagg attggtaacg aaagattctg acggcaatat tcagccgggt gtggcagaaa
      421 gttgggaaaa tacaccggat tacaaaacgt ggacgttcaa attacgccaa gacgctaaat
      481 ggtcaaacgg tgatgccgta actgctcacg atttcgtatt tgcttggcag cgtttggtta
      541 atccacttac ggcttcacct tattcaagct atttaactta tctcaatgta gaaaatgctc
      601 aagatattat tgacggcaag aaaaaaccgg aggaactagc agttaaagcg attgatgatc
      661 atactttcca ggtgcagtta agccaacctg tcccgtatgc agttgagctg gcaacacact
      721 cttctttatt acctgtacac aaagcaaccg ttgagaaaca cggcgatgcg tgggtgcaaa
      781 aaggcaatct ggttggaaac ggggcttata ccttagccga tcacgttatt aatgaaaaaa
      841 tcgtgtttga gcgtaataaa aattattgga atgataaaga aaccgtgatt aataaagcga
      901 ctttcttagc cattgctaat gccgcaactg atgtttcacg ttaccgtgcc ggtgatttgg
      961 atatgaccaa ctatgctctt ccaccggagc agtttgcaac cttgaaaaaa gagattccaa
     1021 acgaagtgtt tacggcaaga accctttcta cttattacta tgaaattaac cataaacgag
     1081 caccgtttga taatgtgaaa gtgcgtgagg cgttaaactt agctttagat cgcaccatta
     1141 ttaccgataa agtgctagga caaggtcaaa caccaactta tgtatttaca ccgccttata
     1201 ttcacgaggg tgaaaaaatc cagcaacctg cttattccaa agattcaatg gcagatagaa
     1261 aagccaaagc gttgaaatta ttggaagaag ccggttacag taaagcgaac ccgttaaaat
     1321 ttactattct ttataatacc aacgacaacc acaagaaaat tgcgattgca gtttcatcat
     1381 tatggaaaca aggcacagac ggcattgttg atgtgaaatt agaaaaccaa gagtggaaaa
     1441 cattcttaga tacccgccgc aatgcaaatt atgatgtttc tcgtgcaggt tggtcggcag
     1501 actataacca agcaaccacc tttgtgaact atttcttatc gaactccagc aacaatactg
     1561 cgttttataa gagccaagct tatgatgatg cggtagcaga atcttacaaa gcgacagacg
     1621 cagctggtcg tgcagctgct tatgcaaaag cagaggagca gttagccaaa gattatgcga
     1681 ttattccgat ttataactat gtaaacccac gtttggtgaa accttacgtg aaaggctatg
     1741 agggtaaaga tccgcaagat gatattttac tgcgtaacct ctacatcgtg aaataagaca
     1801 ttatgggctg tttattgaaa aataagcagc ctatttttat gggggaagaa tggtcgcaaa
     1861 attattctcg gtttacggac gggttcaagg tgtcgctttt cggtttttta ccttgcagga
     1921 agcgaatcga ttaggcataa aaggctatgt cagaaatcgt gatgacggct cggtagaagt
     1981 ggtagctgaa gcggatgaac agacaatcgc tcaactggaa aattggttac aacaaggctc
     2041 acctgctgca aaggtggaac gggtaattgt gagcgattat cttggctcag aaaggtttaa
     2101 aaggtttgag gtaaggtatt aactaacaag cggtgtcatt tgtaaaaaaa tttgcaaacg
     2161 acaccgcttg tattgcttgg tttaatctaa cgctttttca attttctcaa acagatctcg
     2221 tgctaaatta tccagctcac gcaggcgttc tagaccacga cgcatcaagg tttggcgttg
     2281 gttatcgtag cgtgagagtt taattaacgg ctcaataagt cgagcagcaa cttgtgggtt
     2341 actttcgtta agtttaatca gcgtatctac caagaaacga tagcctgaac cgtcaatggc
     2401 gtggaaggct ttcgggtttt gaccgcagaa tgcacctact aatgaacgta aacggttcgg
     2461 gttattaaag ttaaaacttg ggtgttccat taaattttgc acgatggata acacatcgtc
     2521 gtccggtcgg gtggcttgta gcataaacca tttatccatc actaaaccat catgtttcca
     2581 tttttgctca aaatcggcta ataatgcatc acggcaactt agctgggctt tggttgcggc
     2641 ttgtaatgca gcaagcgtat cagtcatatt atcggcatta ttgtaatgct tatgcaccaa
     2701 cgcgttgcct aaatcagtaa aggctaaata gcttaagcaa gcattacgca aggctcgttt
     2761 agcaatatct tcagccacga cttgatattc cccgcattgg ttttggtgat ataccgctaa
     2821 taacttatct tgcaaggcgt gagcaatgga atgctgcata aattcacgca caaccgcaat
     2881 accttgtggg tcaatggttt tgaacgattc tgcaaactcg gtttcttttg gcaatgtgag
     2941 ggttaatgca gttagttccg gcaggcttgc tgagttatcc aacacgaacg ttagggcatt
     3001 caccaaccct tctgagaagc tgagtggttg atgttgctga taacggctta aattttcacg
     3061 cagttcattg gtatagagca tttgagctgc atcccagcgt acaaaatcat tttcagcgtg
     3121 tttgagtaag gttaaaagct gctcggtggt atagttgtaa tctaaccgta ccggtgcaga
     3181 gaaatcacac agtaatgcag gcaccgggcg ttgtgttaca ttatgaaact caaaggtttg
     3241 gtgttctgcg gttacatcta acacatcgct tacggtcaat aattcatatt gtaacggaat
     3301 gcgtttaccg tcctgttcac catataacgc tactttaagc ggaatatgca gattgagttt
     3361 atccatctga tcgtgggttg ccggtgtcat ttgtgaaata tgcaagcggt atgccttgcg
     3421 ggtttcatca tattcatcgg aaatggtgag ctcaggcgtg ccggattggc tataccaacg
     3481 gcggaattgt tcaagattaa cgcctgaagc atcttccatt gctttaacga aatcttcaca
     3541 agttgccgca gaaccatcgt gtcttgccac ataaagttgc ataccttgct ggaatttttc
     3601 ttcgcctaac agggtgtgaa tcatacgaat aatttccgcc cctttttcat acacagtcac
     3661 ggtgtagaaa ttattcattt caatcacttt ttccgggcga atcgggtgag ccatcgggct
     3721 ggcatcttcg gcaaattgta cggtacgcag taaacgcaca tcttcaatcc gttttgccga
     3781 acgagaccat aaatcggagg taaattcctg atcacggaag acggttaagc cctcttttaa
     3841 gcttagttgg aaccaatcac ggcaggtaat gcggttgccc gtccaattat ggaagtattc
     3901 gtgggcaatc accgcttcaa tatctaaata atccgtatcg gttgcggttt ccggttttgc
     3961 caatacaaat ttggaattga aaatatttaa ccctttgttt tccattgctc ccatattgaa
     4021 gaaatcaacg gcaacaatca tataaatatc taaatcgtat tccaaaccga aacgctcttc
     4081 atcccatttc attgaacgtt ttaagctctc catcgcccaa ggtgcacggt ctaaatttcc
     4141 acgatcgaca taaatttcaa gcgtgacttc acgtccgctt ttggtgatga atttatcttg
     4201 gagtaagtca aaatcccccg caaccaaggc aaataaatag cttggtttaa agaacggatc
     4261 ttgccattcc acccagtgtc tgccatcgtg caagtcgcct tgggcaatgt ggttgccgtt
     4321 agaaagcaga tacgggtatt tgctcttact agcggtgatt tttgtcgtat attttgcaag
     4381 tacatcaggg cggtcgagca tataggtaat ctggcggaaa ccttcagcct cacactgggt
     4441 gcaaaacccg tcgcccgatt tatacaaacc ttgtaacgag gtgttttgtg ccggatttaa
     4501 tgcggtttca atttcaagct cgaaccgctc ggcaggcaca tcggctaaat ttaaggttaa
     4561 agattcatca tctttttgaa aggcggaaaa cggctcgccg ttatatttaa gcgaaaggaa
     4621 atcgaaacta tgaccgtcta aacgaagtgt tgtcgcctca ggatttttac gcacgacagt
     4681 tagttttgag gtgacaaaag tgcgatctgg gtcaagttgg aagtcaagat aaatatcagt
     4741 aatagtgaaa tcgggagctc gataatcttt tctgagtttc gctttaggtt gcataggatt
     4801 tccttttttt agttgcgttt agctcataga atagtgtcgt tattgtacta aaatagcggt
     4861 aaattttcca aaaaatttga ccgcttgtcg caataaaaac ggctataaat aggattattt
     4921 atagccgtga gtttatttcg caattaggca gtttggtggc cttggtctaa atgaacgaca
     4981 ttaattaagt catcaacacc aacatcaaag gctttaccaa atccttttac aaataagcct
     5041 gccgttggct taaagctaaa gagcttgaaa tcttgcatat ttgacagttc atcaactaaa
     5101 tcgccgtgac gagctttaag tgctgcaatg ctattattcc attcctcatc agtacgttca
     5161 atcattcgca ctgttgcgtc aaaggttaag cgacgacggg caaaaatttg acggctctta
     5221 ctttcatcct caattagcat taaagagact tttggcacct cttgtaaatt acgagcgtga
     5281 cgggcaatag tagtgattaa gacttgatat tcgccgttat taattacaaa aggggcatag
     5341 ctcacattcg gtacaccatc tttatctatg gttgcaagca ttatagtttt gatttgttgt
     5401 tttaattctt caatttctgg acctaaacgg ttttgtaaga cctcttgacg gttggttgtc
     5461 ataaaatacc tcaat
//
LOCUS       DANLXQ010000159         5441 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.159, whole genome shotgun sequence.
ACCESSION   DANLXQ010000159 DANLXQ010000000
VERSION     DANLXQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.159"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..198
                     /gene="zapB"
                     /locus_tag="PXD94_002235"
     CDS             <1..198
                     /gene="zapB"
                     /locus_tag="PXD94_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL6287054.1"
                     /translation="QLEEKIKQAVETIQLLQLEVEELKGKNDAAKQENDTLRSEYEQL
                     KVEQQNFQDRLRSLLGQIDNV"
     gene            254..835
                     /locus_tag="PXD94_002236"
     CDS             254..835
                     /locus_tag="PXD94_002236"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL6287055.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(926..2044)
                     /locus_tag="PXD94_002237"
     CDS             complement(926..2044)
                     /locus_tag="PXD94_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDL6287056.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSVPPQGYGQFL
                     VDVFHEWLPDRGQIFVIEFDNLLGQWLGYPSNNCVFRPTCGSSLVVEACGDVYSCDHF
                     VYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKLSQACLNCKFRILCHGGCPKHRIV
                     PQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRFAGK"
     gene            complement(2134..3582)
                     /locus_tag="PXD94_002238"
     CDS             complement(2134..3582)
                     /locus_tag="PXD94_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL6287057.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYQ
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            complement(3729..5426)
                     /locus_tag="PXD94_002239"
     CDS             complement(3729..5426)
                     /locus_tag="PXD94_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL6287058.1"
                     /translation="MLLTIITFLVVTGLVGYISWLKTRNDDLSTSKGYFLAGRGLSAT
                     VIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELISYAKKVSAVMAVITMIF
                     GPLLMFGTDGIFLLTKRFAGFVNIPIVALFVIGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGIFLKRATPYEDNKINHANVDLTSWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKAVN"
BASE COUNT         1489 a         1243 c         1242 g         1467 t
ORIGIN      
        1 caattagaag aaaaaattaa acaagctgtt gaaactattc aattacttca attagaagtt
       61 gaagaattaa aaggtaaaaa cgatgcagcg aaacaagaaa acgacacctt gcgcagcgaa
      121 tatgaacaat taaaagtgga acaacaaaac ttccaagatc gtttacgttc acttttaggt
      181 caaatcgaca acgtttaatt ctattttcag cacaaacggc taaattttta gccgtttttt
      241 atttgttaca gctatggcaa aactctattt ttactactcc tcaatgaatg caggcaaatc
      301 tactactctg cttcaatctt cttataatta ccaagaacgt ggaatgaaca ctttagttta
      361 taccgccgct attgatgacc gtttcggtgt ggggaaagtc aactctcgta tcggtatttc
      421 acaagaagcc aatttattta ccgctgatac caatctattt gcggaaatta aggcattaca
      481 ctcaaacaaa gcgttacact gtatcttgat tgatgaggct cagtttttaa ctaaaacaca
      541 ggtttatcaa ttaagtgatg tggtcgataa actcaaaatt ccggtgcttt gctacgggct
      601 acgcaccgat ttccaagctg aattatttga aggtagcaaa tatttgttgg cttgggcaga
      661 tgagctggaa gaactcaaaa ccatctgtga ttgtggcaaa aaagcccatt ttgtcgttcg
      721 attaaatgaa aaaggcgaag ccattaaaga cggtgaacaa atacaaatcg gcggcaatga
      781 tagatatctc tctgtttgcc gttatcatta caaacaaaag ttaggtaagc tataagctac
      841 aagaagcagc aagcggttaa attttcggga atttttgcaa attttcagcg aaatttaacc
      901 gcttgtttgt gttatttacc tctctttact ttcctgcaaa ccgaatttgc tcactcattt
      961 gtctcatcac actcgaagtc tcggcaaaaa actgttggta agaggggcaa aggtagttgt
     1021 ggttttcgct gtttgcttgg gggacgatgc ggtgtttggg gcagccgccg tggcaaagta
     1081 tgcggaattt gcagtttagg caggcttgcg agagttttgc ctgtttattc tgcccgaatt
     1141 ggcgttgggc ggctgatgag acgatctctg ccagctgctg ttggttgagg ttgccgatgt
     1201 ggtaatcggg atagacgaaa tggtcgcaac tgtacacatc gccacaggct tccaccacca
     1261 gcgagctgcc acaggtcggg cggaatacac agttgttgga gggatagcca agccattgcc
     1321 cgagcaggtt atcaaactcg atcacaaaaa tctgcccacg gtcaggcagc cattcgtgaa
     1381 aaacatccac taaaaattgc ccgtagcctt ggggcggaac ggaaaagggt tgtaattccc
     1441 ccttgtggct ttccaccacc ggaatgaact gcatataacg gctaccgagg gctttgaggg
     1501 cttgataggt ttctctgccc cgttgccagt tgcggtcgtt caccaccgtg agggtgttaa
     1561 attccacgcc aacctctttc aataaatcga ccgcactttt cacccgttca aaggtcggtt
     1621 taccgttggt ggaaatgcgg tagcggtcgt gaatgtcgct cagtccgtca atggataagc
     1681 ccaccagaaa atgattctct ttgaaaaagc tcgcccactg gcggtttagg gcaatgccgt
     1741 tggtttggat tgcgttggtg atctgcttgc cgtttgcaaa tttttgttga tatttgaccg
     1801 cttgtttgta gaaatctaag cccagcaggg tcggctcgcc cccctgccac acaaaatcca
     1861 cccgttggct ttggttggct tcaatgtaat gacgaatgta gttttgcagc gtttcatcgt
     1921 tcataaactg cgatttttct ggcggaatgg tctgttcctt gctcagataa aaacagtact
     1981 cgcactgaat attacagtga aaactgctgg gttttgccat taggtggaag taggtggtgg
     2041 tcattgtatc tctaaattgt aggggcggac tttatgttcg cccattgttt acattaattt
     2101 tcgggcgggt ataaaacccg cctctacaaa caattatatc tcatcaataa tccgatacac
     2161 ccaattttcc agtgggtcgt tgaggcgttt catttcctgt cgcatttctt ccaacatctc
     2221 ttttttcacg gcttggtagg cttcgtcata aaagaggttg tgcatctctt cggggtcttt
     2281 tctgacatcg tagagttcgc agcggtcggt ggcgttgaag accaatttgt aatccttacg
     2341 ccgccacatt cgctgttcaa aatagacaaa atgccctgcc agttggcaca acaagccttt
     2401 gcggtcgttg ttttggcttt gtctggttat cggcagaata ctctcgcctt ggaaagcggt
     2461 cgggatcggt tgatttgcca catcatacac ggtggaagtg agatcgtgca aataaacgag
     2521 gttgtcatcg acttcgttca cccttgtaga gtgcggatct ttaataatca gcggaatgtt
     2581 gtaggtggtt tcaaacataa actcgccttt ttcgatcatc ttatgcgccc ccatcgcatc
     2641 gccgtggtcg gcggtagcaa cgaggaaggt attttcatag agattgttct cttccaagaa
     2701 acggaacagc tcgccaatgg catcgtcaat cagcgtgatg tagccccaga atttagaaat
     2761 cacctctttc caacgatctt cgctcgcctc ccacattccc cacatttgtg aaatggtgcg
     2821 gaagtggttc ggtttgccct caagcggttt gaagaaactc tcgtccaata ccacttggct
     2881 tgggtcatac attgaataat acggctccgg cactacacac ggagtgtgcg gcccccagaa
     2941 gttgatccac gcaaagaacg gttttttctc atcaagggag gcttgaatgt attttttcgc
     3001 ctcgtcaatg ataaaccact ctattgtctg ctcgcgactg ccggaaagca aaccacagag
     3061 ttcctgcaca cgcaggtgtg gattgtcgcc aaaataggct cgactgactt cgggtgcgtc
     3121 atagcccttt tccgccagcc attcacgata acggttggag tgtgttggcg gctgattaaa
     3181 cacgaggttt ttatacactt ggctgcccgg gtagccatag ccgtcaaagt tatgaccggc
     3241 gatgccataa tcttcgggaa cggattttgt accaacgtgc catttgccga tcacaaagga
     3301 gttatagccc tccacgccgg caatattagg gttttgttgt ggaatttcgc ctgtgccgcc
     3361 tttttcgccg tttttgataa tcccgtggct ggacggcatt aagccggtaa acagcgaggt
     3421 gcgagccggc ccgcagaccg aagccggcgt aaaggcgttg ttaaaccgaa tgccgtcttt
     3481 cgccaagcga tccaaattcg gggtttttac cacctgatgc ccgtaagtgc cgagcatatc
     3541 tttacgcact tgatctaaca aaatatagat gatgttattc ataatgacct ctttaaggct
     3601 tgttaataac cgataaatta tcaagtttcg gagatctgtt cccctcttta gcaaagaggg
     3661 gatttttgat ctcgagcctt ttccgttctt ggtattttta tactaatgag gagaaggctc
     3721 acattcattt aattcaccgc ctttttgcct ttacattttg caaaaaacac gacacaaacc
     3781 accgcttgta gcacagcata caccgccagc accattgccg gattgccgtc tggtgaggct
     3841 aaaccgagcg gagagaggaa agcgtatagc gtgaataagc ccaacacgat agaagccact
     3901 gccagtgggg catattccca gctggttaag tccacattgg cgtggttgat tttgttgtct
     3961 tcgtatggag tcgcacgttt taggaaaatt cctaaaatca gcattaacgc cacatcgaac
     4021 acgaacaagc cagcttgcac atacacgaag ttgattttta catcaaacac ccaaacgatg
     4081 cagaagtaga gaataacgtg tgcgagtaag gcaatgcgtg cggctttgcc tgagacggtt
     4141 ttgttgaata agccgatgac aaacagtgcc acaatcggaa tgttcacaaa accggcgaaa
     4201 cgtttggtca gcaagaaaat accgtctgtg ccgaacatca gtaacggccc aaagatcatc
     4261 gtaatcaccg ccattaccgc agagactttt ttcgcataac ttatcagttc cgcatctgtg
     4321 aactgttttt tgctgataga cggcagtaag tctttacagt aaatggttgc cgctgagttt
     4381 aagaacgagt tgaaggtact caaaatcgcc ccgaacagtg cggcaataaa gaagccttgt
     4441 agcacggttg ggaaagtttt attcactaat tcagggtaag acacatcaat cggtttcaag
     4501 ccctcaccta aaatatggaa actgagtaag cccggtaagt ttaagataag cggtagcaat
     4561 aataagaaca gtgccgcaat taagatccct ttttgaccgg cttttaagtc tctcgccccc
     4621 aaggcacgtt gcacaatggc ttggttggtc gtccagtaga agaaatgcac caccataata
     4681 ccggtgaaaa tggtcggcca aggcactgca tcggtggcac tacccaccgc attccatttt
     4741 tcaacgtgag tagtggtgat gattttcacc ccttctgcca tactgccatt acctaaatag
     4801 ataagtgcaa acaccggcac taataatgcc ccaatgacta acaagaccgc attccaagtg
     4861 tctgacaccg ccaccgcttt taagccaccg aaaatggcat aaatcgcccc aacaatacca
     4921 atcgcaatca ccgtataggt aatcgctgcc ccatagctta agccgaaggc agtttcaagg
     4981 ttgaagattt tattgaaggc aatcgcaccg gtgtaaagtg cagtcggaat gacgatgaaa
     5041 agatagaaca ccaaaaacaa gcccgacata atcaaacggg tttggcggtc gaaacggttt
     5101 tcaaagaact ccggaatggt ggtataaccg ttgcggatat atttcggcaa cagataaagt
     5161 gccaagaagc aaagcggaat taccgccggc acagtccacg caataattga gaaattaccg
     5221 gcataagagt tggcattcac gccgataagt tgctcggtag aaagggaggt taagaccatc
     5281 gaacagccga taaccgtagc actcaagcca cgccctgcca agaaataacc tttagatgtc
     5341 gaaagatcgt cgtttctggt ttttagccaa gagatatacc cgactaatcc agttacaacc
     5401 agaaaggtaa ttattgttaa taacatattc ttcacctcaa t
//
LOCUS       DANLXQ010000160         5344 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.160, whole genome shotgun sequence.
ACCESSION   DANLXQ010000160 DANLXQ010000000
VERSION     DANLXQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.160"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            70..915
                     /locus_tag="PXD94_002240"
     CDS             70..915
                     /locus_tag="PXD94_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6287059.1"
                     /translation="MKEKIIKTNGIELCTESFGNKKNPAILLVAGATVSMLYWDTEFC
                     QQLSEKGFFVIRYDNRDVGKSTNYEPGSTPYDIVDLTNDAISILDGYKIDKAHFVGIS
                     LGGLISQIASIKFADRVNSLTLMSSGPWGDSDPTIPEMDTSILDFHSKAGTVNWTNED
                     SVVNYLIQGAELMSGKKQFDKQRSEKLIRAEFNRANNYISMFNHAALQGGGEEYWNRL
                     NEIKQPTLIIHGTDDKIWHYKNAGFLLEKIKGSNLITLEGTGHELHVDDWKSIIDGIE
                     KHIND"
     gene            complement(1240..2646)
                     /locus_tag="PXD94_002241"
     CDS             complement(1240..2646)
                     /locus_tag="PXD94_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL6287060.1"
                     /translation="MIVKFFKKHGKGKASSCKACVDYLLNKPDDTAQILQGDPRLSQS
                     IADSLDFNNTYTAGCLSFEESDLPEIQKREIMARFEKAMFAGLEPEQYNIAWVQHTDK
                     GRLELNFVIPNVEMTSGKRLQPYYDRADRPLAENFKQVINHEYSLSDPNNPIKQKNLI
                     DRKDLPTDKKQALQAITDGLTALANAGQINDRQDVINALERAGFEIARITPKNLSIKT
                     DGQNLRLKGAFYEQDFRFSTDLSADITERAREYKRDSAERYQTARAKLDTAVTARREQ
                     FSRKYPNRAGEIDKKYRENVSLADPNRLDDISLDSHNRRRFDVAGEERLSRDDRMAKF
                     SRDSQRAEWGDQAINLQNGERDKALCSSGQGFYQLNVGREPESNRNPRNQETGEKING
                     DRTRTTLRENLENLARTARNRASEIIERIRELASRKRLDHSTVQRNQRAINDITEFNK
                     QLETVISQRKRQSRGMSR"
     gene            complement(2643..2948)
                     /locus_tag="PXD94_002242"
     CDS             complement(2643..2948)
                     /locus_tag="PXD94_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL6287061.1"
                     /translation="MKREKEIKIRLTENEYQALLERKTKARLAEWVREVALEQQPKRQ
                     PKVIDPALLFELNRIGVNLNQIARQCNSQKPSIDLVSVLATLREIEKNLKKLRELSL"
     gene            4142..4279
                     /locus_tag="PXD94_002243"
     CDS             4142..4279
                     /locus_tag="PXD94_002243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287062.1"
                     /translation="MKPLRIGARVIYPLQDLDKQIAYLEANLLENIKVHFIVESIEIK
                     G"
     gene            4414..5331
                     /locus_tag="PXD94_002244"
     CDS             4414..5331
                     /locus_tag="PXD94_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class A beta-lactamase
                     ROB-1"
                     /protein_id="HDL6287063.1"
                     /translation="MLNKLKIGTLLLLTLTACSPNSVHSVTSNPQPASAPVQQSATQA
                     TFQQTLANLEQQYQARIGVYVWDTETGHSLSYRADERFAYASTFKALLAGAVLQSLPE
                     KDLNRTISYSQKDLVSYSPETQKYVGKGMTIAQLCEAAVRFSDNSATNLLLKELGGVE
                     QYQRILRQLGDNVTHTNRLEPDLNQAKPNDIRDTSTPKQMAMNLNAYLLGNTLTESQK
                     TILWNWLDNNATGNPLIRAATPTSWKVYDKSGAGKYGVRNDIAVVRIPNRKPIVMAIM
                     STQFTEEAKFNNKLVEDAAKQVFHTLQLN"
BASE COUNT         1514 a         1078 c         1095 g         1657 t
ORIGIN      
        1 aaaaaaaaac tactttggat attaatttta ggactgataa taatcagttg caaacaaagg
       61 aaaacagaaa tgaaagagaa aataattaaa acaaacggca ttgaactctg tacggaaagt
      121 tttggaaata agaaaaatcc agcaatcctt ttggtagcag gtgcaaccgt atcaatgctg
      181 tattgggaca ctgaattttg ccaacaatta tctgaaaaag gattttttgt tattcgttac
      241 gacaacagag atgtaggaaa atccactaat tatgaaccag gttctactcc atacgatatt
      301 gttgacttaa ctaatgacgc tatttcaata ttggatggct acaagattga caaagcacat
      361 tttgtgggga tttctttggg cggactaatt tctcaaatag catcaataaa gtttgccgac
      421 agagttaact ccttaactct tatgtcatca ggcccttggg gagactcaga cccaactata
      481 cctgaaatgg acacgagtat tttagatttc catagtaaag caggtacagt caattggaca
      541 aatgaagaca gtgtggtaaa ctatttaatt cagggtgcag aattaatgag tggcaagaaa
      601 caatttgaca aacaaagaag tgaaaaactg ataagagctg agttcaatag agccaacaat
      661 tatataagta tgttcaatca cgctgcattg caaggtggtg gtgaagaata ttggaacaga
      721 ttaaacgaaa tcaaacaacc caccttaatt atccacggaa cagacgacaa aatttggcat
      781 tataagaatg caggtttttt actagaaaaa ataaaaggtt caaatctaat cacccttgaa
      841 ggtacaggac acgaattaca cgttgatgat tggaaatcaa taattgatgg aatagaaaaa
      901 cacataaatg actgataaac aaaaacaacg acccgctaac aatgtatata aaaaataggc
      961 gaaacagtag taaattcaag gcttttggct cgtattatac tttgtgctta accgaaaatt
     1021 tggcacttcg aaatcgccta cttttcgctg aatttacaat ccaagtgcaa caaaaaaaga
     1081 agtactcatc aactagaata taattttgtt tccacacaaa aaccacttcc aaatgatgag
     1141 tacttcctac cgacatctta caataagccg agtgtttgag aaataccatt tattaaactg
     1201 tctttttcca taagtgttgc cataattctc ccccttgttt tatctgctca tacccctgct
     1261 ttgtcttttg cgttgagata ttaccgtttc aagctgttta ttaaactctg taatgtcgtt
     1321 aattgctcgc tgatttcgtt gtactgttga atgatcaagg cgttttcggc tcgcaagctc
     1381 tctaattctt tcgataattt cactcgctct gtttcttgct gttctagcaa ggttttcaag
     1441 gttttctcgt aaagtagttc tagttcggtc gccatttatt ttttcccctg tttcttggtt
     1501 tcttggattt cgattacttt caggttctcg ccccacgttg agttgataaa atccttgtcc
     1561 actgctacac aaggctttat ccctttctcc gttttgcaag ttgatagctt gatcccccca
     1621 ctctgctctt tggctatctc ggctgaattt tgccattcgg tcatctcttg ataggcgttc
     1681 ttcgcctgcc acgtcaaaac gcctgcggtt atggctatca aggctaatat cgtcaaggcg
     1741 attagggtcg gcaagcgata cattctcacg gtatttttta tcaatttcgc ctgctcgatt
     1801 tggatatttt cggctaaatt gttccctgcg tgctgtaact gctgtatcaa gttttgctcg
     1861 tgccgtttga tagcgttctg cactatctcg cttgtactct ctagctcttt ctgtgatgtc
     1921 tgctgaaagg tctgtgctaa atctaaaatc ttgctcatag aaagcccctt ttaatcttaa
     1981 attctgtccg tcagtcttga ttgataggtt ttttggcgta atgcgtgcaa tttcaaaacc
     2041 tgcacgttct agggcattta taacgtcctg tcggtcgttt atctgccctg cgttcgctaa
     2101 agctgttaaa ccgtccgtaa tcgcctgtaa agcctgtttt ttatcggttg gtaggtcttt
     2161 gcggtcaatc aggttttttt gctttatagg gttgtttggg tcgcttaggc tgtattcgtg
     2221 gttgattacc tgcttgaagt tttcagcaag tgggcggtct gccctgtcgt agtagggttg
     2281 taggcgtttt ccgcttgtca tctctacgtt tgggataacg aaattcagct caagcctgcc
     2341 tttgtcggtg tgttgtaccc acgcaatgtt atattgttca ggctcaagcc ctgcaaacat
     2401 tgccttttca aagcgtgcca taatctcacg cttttgtatt tcgggtaggt cgctttcttc
     2461 aaaagacaag caacctgcgg tgtaagtgtt attaaaatca agactatcag caatactttg
     2521 tgatagtcgg ggatcgccct gcaggatttg ggcggtgtcg tcaggcttat ttagtaaata
     2581 gtccacacaa gccttgcaac tactagcttt tcccttaccg tgtttcttaa aaaatttaac
     2641 gatcataggc tcaattctcg caattttttg agattttttt caatttctcg caaggtcgcc
     2701 aacacgctaa caaggtcaat gctcggcttt tgactgttgc attgtcgggc gatttggttc
     2761 aggtttacgc ctatgcggtt cagctcgaac agtaacgcag ggtcgattac tttcggctgt
     2821 cgcttaggtt gctgttctag ggcaacttcc cgaacccact ccgcaagcct tgctttcgtt
     2881 tttctctcta acaaggcttg atactcgttt tcggtgagcc tgatttttat ctctttctca
     2941 cgtttcataa tggtgtccgt tcttctattt tgaccgctga aagcggtcgg gggtcaaggg
     3001 ggcaaagccc ttttgcaagg tcaaaaagga aactcgaaga gtaccctttt tgtgtccttg
     3061 ctattaaaaa agacaatagt acaccaaaag ccccgagagt gatatataaa aatcagcgga
     3121 gccgataggc gacttcgttt tattaggcga acatcgtgag cctttgaacc aatataaaaa
     3181 taaaaaaatt tattattagg gaagtggtta ttgtgggcgg taggttttta gtgttggaaa
     3241 gctgttggaa agctcgtggg taactcgcag agttatccat cagcttttca atggcttttc
     3301 aatacgaccc gcaggggaaa aaccgttaaa ccgtccacat taaccgctga cccaaaaaga
     3361 acgcttttta ctaaaagatt tctattcggt tctcaaatgt tgcgtgaatg gggcaacaat
     3421 aaaacgttgt ggcgtttatc agggtgggaa ttgcagactt aaacaatcaa ttccgctgta
     3481 tccttaaatc tcgcttatca ggtgtgcttg tatttcttgg ggctttcgcc tttctaaccg
     3541 tagcacgaaa cggagacaga gcttaatttt cgtattaaga acgttatcac ttctacgcct
     3601 gtttattctt ccgaagtccg agctgaagac cgtctaattt aaggtttacg tttcttgttt
     3661 acgtcgttga atggtttttt ggtagtaggg acgcttcgca atctcttgca ttactagcta
     3721 ccctttgcta tgattagatt gttcacgtct ggatcatagc aaagaacgta ctgatacaga
     3781 ttataggaaa agggatcgaa tgtcaaaagt tcgatccctt tttcttttct aaagcattgg
     3841 tattaaaggc tttaaggtgt attttattaa aatctgtaca cctttgtgaa aatccataca
     3901 cctttcttac cgtgtaattt tatgaaaatt cgtgcacctt tctgctaacg ccctgcggtt
     3961 gttagactaa aaaagccatt gaaagcaagt ttcaacggct ttttcagtcc cgaataaaac
     4021 aaaaccgcct tgttaggcgg ttatgtgtta aaatcacttt cgcactatgg cggcggctcg
     4081 cactaccgtt catagtgtga gcctagtcgc ttagccttat agtgctaagg gggtgattta
     4141 tgtgaaacct ctacgaattg gggcaagggt tatttatccg cttcaagact tagacaagca
     4201 aattgcatac ctagaggcga acttacttga gaatatcaaa gtgcatttca ttgtagaaag
     4261 cattgagatt aaaggctaaa ttgctagact tttgcgctta aattcgccaa aatctgtttt
     4321 tttgttctac aaacaaatta tccgaatttc cgcgcaatgt acgtttcagg ctgcctgaaa
     4381 agaaatttat ttagtaaaat caaaggataa tttatgttaa ataagttaaa aatcggcaca
     4441 ttattattgc tgacattaac ggcttgttcg cccaattctg ttcattcggt aacgtctaat
     4501 ccgcagcctg ctagtgcgcc tgtgcaacaa tcagccacac aagccacctt tcaacagact
     4561 ttggcgaatt tggaacagca gtatcaagcc cgaattggcg tttatgtatg ggatacagaa
     4621 acgggacatt ctttgtctta tcgtgcagat gaacgctttg cttatgcgtc cactttcaag
     4681 gcgttgttgg ctggggcggt gttgcaatcg ctgcctgaaa aagatttaaa tcgtaccatt
     4741 tcatatagcc aaaaagattt ggttagttat tctcccgaaa cccaaaaata cgttggcaaa
     4801 ggcatgacga ttgcccaatt atgtgaagca gccgtgcggt ttagcgacaa cagcgcgacc
     4861 aatttgctgc tcaaagaatt gggtggcgtg gaacaatatc aacgtatttt gcgacaatta
     4921 ggcgataacg taacccatac caatcggcta gaacccgatt taaatcaagc caaacccaac
     4981 gatattcgtg atacgagtac acccaaacaa atggcgatga atttaaatgc gtatttattg
     5041 ggcaacacat taaccgaatc gcaaaaaacg attttgtgga attggttgga caataacgca
     5101 acaggcaatc cattgattcg cgctgctacg ccaacatcgt ggaaagtgta cgataaaagc
     5161 ggggcgggta aatatggtgt acgcaatgat attgcggtgg ttcgcatacc aaatcgcaaa
     5221 ccgattgtga tggcaatcat gagtacgcaa tttaccgaag aagccaaatt caacaataaa
     5281 ttagtagaag atgcagcaaa gcaagtattt catactttac agctcaacta acaaattcag
     5341 catg
//
LOCUS       DANLXQ010000161         5306 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.161, whole genome shotgun sequence.
ACCESSION   DANLXQ010000161 DANLXQ010000000
VERSION     DANLXQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.161"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            280..753
                     /locus_tag="PXD94_002245"
     CDS             280..753
                     /locus_tag="PXD94_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL6287064.1"
                     /translation="MLEKITACPCQTGKRYAECCQPFHLKQTFASTAEQLMRSRYSAY
                     TLVNIPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSRIHSQVEFKAF
                     FDTDEGIQGHHETSLFVQIDERWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
     gene            825..1454
                     /locus_tag="PXD94_002246"
     CDS             825..1454
                     /locus_tag="PXD94_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL6287065.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAVAIGL
                     ISMLFGRNGGSSLAKLGSLAAIGSLAYQAYQKYQAQNSSVSELSQNNFENVDENVASK
                     VILQAMIAAAASDGAITEEEKAAIVSQVGDEAEVIEWIRQEMDTPATVEEIARQVGND
                     QALATQVYLASRAVCRDLERKEIVFLANLADALGLAEALVEQLEKDAGF"
     gene            complement(1532..2179)
                     /locus_tag="PXD94_002247"
     CDS             complement(1532..2179)
                     /locus_tag="PXD94_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6287066.1"
                     /translation="MISIRNVSKQYRTNNGWKTILKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPTTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYDADFDYV
                     KSFTEEFSELGEYLYEPVKSYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKHELFEKRKDRSLILVSHSPASIKEYCDSAMVLEEGIMHTFDSIDNAYNFYNKT"
     gene            complement(2176..2973)
                     /locus_tag="PXD94_002248"
     CDS             complement(2176..2973)
                     /locus_tag="PXD94_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL6287067.1"
                     /translation="MRYGDNTTFKRSCVIQKRVIHALLMRELITRYGRKNIGFLWLFI
                     EPLLLTLVITVAWKYIKADKFSTLNIIAFMITGYPIAMMWRNASNRAQGAISANLSLL
                     YHRNVRVLDTIFTRVLLEVTGASIAQILLMTIFIAIGWIEMPNDIFYMLCAWILMAFF
                     ALGLGLIICTLAHYIEPFGKIWGTFSFFLLPLSGAFFFVHNLPLTAQNIALWFPMIHG
                     TEMFRHGYFGETVITYESISYLIVSDIILLFIGLLCVRSFSKGIEPR"
     gene            complement(2973..4076)
                     /locus_tag="PXD94_002249"
     CDS             complement(2973..4076)
                     /locus_tag="PXD94_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL6287068.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGLGFNDTQEAFYRYFKERLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSSV
                     QLSLISTLKSELIRVETQLAQLKSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLAAAMTSLQNTRGEADRQQLYLEVIGRPSKPDWALE
                     PYRIYNIISTFFIGLILFGVLSLLLASIREHKN"
     gene            complement(4148..5296)
                     /locus_tag="PXD94_002250"
     CDS             complement(4148..5296)
                     /locus_tag="PXD94_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL6287069.1"
                     /translation="MTLSLISACSSLPTSGPSYSEILASNENTAETQLPEVNLIKLDN
                     ITVQNLYQEQQSQRFSGFDGSMGVGGYAGTVNVGDILEISIWEAPPAVLFGGTFSAEG
                     QGNGHLTQLPQQMVNKNGTVTVPFIGNIKVAGKTPEVIQNQIVGALSRKANQPQALVR
                     IANNLSSDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDVTVRLTRGNKVRS
                     MAFETLIADPSQNITLRSGDVIALMNTPYSFTGLGAVGNNQQMRFSSKGITLAEAIGK
                     MGGLIDERSDPRGVFVFRHIPFTQLDYDNQAQWREKGYAEGMDVPTVYQVNLLKPQSM
                     FLLQRFPIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIRAY"
BASE COUNT         1698 a          990 c          933 g         1685 t
ORIGIN      
        1 tttctgtgaa caaaagatct tttcatttaa aaacaataag ttagtataat ttaaataggt
       61 gaatactatc aattaaccaa taaattaacc tgtggataaa atcttaagtt atccacaatc
      121 tatagaataa aggcttttta aagcaatgaa tacccaaaaa attcacgatt tttgcataag
      181 ttatccacaa ggggtatgtc acgctttttt ataaaattta tttttcttta tttatagcgt
      241 aaacttttca catctttatt tttatgcgga aatgtaaaaa tgttagaaaa aatcaccgct
      301 tgtccttgcc aaacaggcaa acgctacgct gaatgttgcc aacctttcca tttaaaacag
      361 acatttgcat caacggcaga gcaattaatg cgctcccgtt acagtgctta tactttggtt
      421 aatattccct atattgtgga aacgacagtg ccaagtcagc agaaattact tgatcagact
      481 gcaatgaaag agtgggctga aaatacaaaa tgggtaggat tatccgtgct aaatcactta
      541 ccttctcttt cgagaataca tagtcaggtt gaatttaaag ccttttttga tacagatgag
      601 gggatacagg gacaccacga aacctctctt tttgttcaaa ttgatgagcg atggtttttt
      661 gtggatccta ccgtaccttt gcctcatcaa aaacaacctt gtgtatgtgg ctccggaaag
      721 aagtttaagc actgttgtgg aaattatctt taatttgaat tgaactcttg tttgaatcta
      781 aggactaatg agtaaatttc atttacttac caagaggaac aaaaatggat ttcagtaaga
      841 ttttaagtca agtattagat acagcaaaag atgccgctac aaacggatta attaaaggta
      901 attctaaaaa cgatcaaatc gctaaaattg gtggcggcgc tgtcgctatt ggtttaattt
      961 caatgttatt tgggcgtaat ggcggttcaa gtttagcaaa attaggctct ttagccgcta
     1021 tcggtagcct tgcttatcaa gcataccaaa agtatcaagc gcaaaattca tcggtttcag
     1081 aacttagcca aaataatttt gaaaatgtag atgaaaatgt ggcaagtaaa gtgattttac
     1141 aagcaatgat tgccgctgct gcttcagatg gtgctattac tgaagaggaa aaagcagcta
     1201 ttgtttccca agttggagat gaggctgaag tcattgagtg gattcgtcaa gaaatggaca
     1261 ctccggctac agttgaagag attgcccgcc aagtaggtaa tgaccaagca ttggctacac
     1321 aagtttactt agcttcccgt gcagtatgca gagatttaga acgcaaagaa atcgttttct
     1381 tagctaattt agctgatgct ttaggtttag ctgaggcatt agttgaacaa ttagaaaaag
     1441 acgcaggttt ctaattttca gttttagtaa caagcggtta tattttgaga gttttttgcg
     1501 aaaaacttgt gaaatataac cgcttgttct tttatgtttt attgtagaaa ttataagcgt
     1561 tatctataga atcaaaagta tgcatgatac cctcttctaa aaccattgca gaatcgcaat
     1621 attctttaat tgaggctggg ctatgagaaa ctaaaatcaa tgaacggtct ttccgttttt
     1681 caaaaagctc gtgcttacat ttggcagcga atcgagcatc gcctactgca atcacttcat
     1741 caattaagta acaatcaaat tcaatagaaa gcgataatgc aaaggctaaa cgggccttca
     1801 taccagaaga atagcttttt accggttcat ataaatattc accgagttct gaaaattctt
     1861 cggtgaaaga ttttacatag tcaaaatcag catcataaat tcggctgacg aaacgaagat
     1921 tatccattcc ggtcaaacta ccctggaatg caccactaaa tgccagtggc caagaaatcg
     1981 acatttgacg ttcaatcgta cctgttgttg gaggttctac tccgctcatt aaacggatta
     2041 aggttgattt tcctgcacca ttgcgcccta aaatccccac tttttcgcct tttttcagct
     2101 caaaatttat atcttttaat atggttttcc aaccgttatt agttcgatat tgcttactta
     2161 catttctaat actaatcatc ttggttctat ccctttacta aaacttctca cacaaagcag
     2221 tcctataaat agaagaataa tatcacttac tataagataa ctaatgctct cataggtaat
     2281 taccgtttca ccaaaataac catgtctaaa catttctgta ccatgaatca ttggaaacca
     2341 taaggcaata ttttgagcag ttagcggtaa attatggaca aagaagaatg caccagaaag
     2401 aggaagtaga aagaagctga atgttcccca aatcttacca aaaggttcaa tataatgtgc
     2461 taaagtacag ataattaaac ctaaacctaa ggcaaaaaat gccattaata tccaagcaca
     2521 gagcatatag aaaatatcgt taggcatttc tatccaacca atagcgataa atatagtcat
     2581 taagagaatt tgggcgatag atgcgccagt aacttctaat aaaactcgag taaatatcgt
     2641 atctagcact ctcacatttc tgtggtaaag caaacttaag tttgctgata ttgctccttg
     2701 agctctatta gaggcatttc tccacatcat tgcgataggg tagcctgtaa tcataaaagc
     2761 gataatattt aaagttgaaa acttatctgc ttttatatat ttccaagcaa cagtaatcac
     2821 taacgttaac aataatggtt cgataaaaag ccataagaag ccaatatttt tacgcccata
     2881 acgagtaatt aattccctca ttaaaagggc gtgaatcacc cgtttttgaa tcacacagga
     2941 acgtttaaag gttgtattat caccatatcg cattagtttt tatgctctct aatacttgct
     3001 agtaacaaac ttaatactcc aaatagaatt aagccgataa aaaaagttga tataatatta
     3061 taaattctgt agggttctaa tgcccaatct ggtttgcttg gcctaccaat tacctctaaa
     3121 taaagttgtt ggcgatcagc ttcacctcgt gtattttgta atgaagtcat tgctgctgca
     3181 agttgttgct gagctaattc gttagcaaga accaaacgtt gatattcagc agtttgcgtt
     3241 gctacagaag tattacctcc tgcaagttgt ttggactgat catcaatttc ttttttcaaa
     3301 cttttttgac gcatttgtaa tgcggctacc tgaggattat ccggcgtaat tgacttcaat
     3361 tgtgctaatt gtgtttctac ccgaattaat tcacttttta aggtagaaat taatgagagc
     3421 tgaacgctgg attgtgccgg taaatcataa attttatttt tagttcgata ttggcttaat
     3481 gctaacgcag tattattgac gtctttttcg gcctctttta cacgctcttc tgcaaactca
     3541 accgtatctt gtcttgctct ttcattaagg cggttaatta atgtttcccc ttgctgtaat
     3601 aaattctcgt taattttttg tccttcttct gcattaaaag cacgaacgcg tagcgttgca
     3661 atacctgaaa tagaatcgac atcaatattc agacgttctt taaaataacg ataaaatgct
     3721 tcttgggtat cgttaaatcc taaaccgtta aatttgctta gtaaatcacc ttgctcacta
     3781 taaaaacgct ttaccggaat ttctgcttca agttgttcta atgcggtacg ggaacgcata
     3841 tattcctgaa ctgaataggt atcatcttga gaacgggcga aaccggagtt ttgcaataaa
     3901 gcaccaacgc ctgttaagct cgattgatta ttgggcgaac gaaccacaaa gctagattct
     3961 gaaacataaa tatcagaggc aaataaggaa taataaattc cagaacaagc agttggaata
     4021 attacagtta gccacaacaa agggttggct aaaaaatgtt ttttcttctt tgacataaaa
     4081 tcctcaacga aaagtgttac aagcggtcat tttttaatga ttttttacca ttttatctat
     4141 gagctaatta gtacgctcta attgtattag ctgtacctgt aattggtgta gtaatggaga
     4201 aaatcattct caagaatttt tgaaattcag acagtggagc attcgacaca tatacaatat
     4261 ctttatcttg aatagggaaa cgctgcaata aaaacattga ttgcggtttt aataaattca
     4321 cctgataaac agttggaacg tccattccct ctgcataacc tttttcacgc cattgagctt
     4381 ggttatcgta atctaattgt gtaaaaggaa tatggcggaa tacgaacaca ccacgagggt
     4441 ctgaacgctc atcaattaat cctcccattt taccgatagc ttcagcaagg gtaatgcctt
     4501 tacttgaaaa acgcatttgt tgattattac ctactgctcc taatccggta aagctataag
     4561 gagtattcat taaagcaata acatcacctg aacgtaatgt aatattttgt gaggggtcag
     4621 caattaatgt ttcaaacgcc attgaacgca ctttatttcc acgtgttaaa cgaactgtta
     4681 catcttcaat attttcgctt gtaccaccta ctgccgcaac tgcatctaaa atacgctcat
     4741 tattggcact taatggcata cgaatgctat ttccttggcg gataaccgta acatcagacg
     4801 acaagttatt cgcaatacga actaatgctt gaggttgatt agctttacgg ctcaatgctc
     4861 cgacaatttg attctgaatt acttcaggtg tttttccagc aactttaata ttaccgataa
     4921 aaggcacagt taccgttcca tttttgttca ccatttgctg aggtaattgg gttaaatgcc
     4981 cattaccttg cccttcagca ctaaatgttc caccaaatag cacggcaggt ggcgcttccc
     5041 aaatggatat ttctaatata tcgcctacat taactgttcc agcataacca cccacaccca
     5101 ttgagccatc aaatcctgaa aaacgttgag attgttgctc ttggtataag ttttgtacgg
     5161 tgatgttatc taacttgatt aaattgactt caggcaactg cgtttcagct gtattttcat
     5221 tacttgctaa gatttcgcta tagcttgggc cggacgttgg taggctagag caggctgaaa
     5281 ttaatgataa tgttataaat aataat
//
LOCUS       DANLXQ010000162         5285 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.162, whole genome shotgun sequence.
ACCESSION   DANLXQ010000162 DANLXQ010000000
VERSION     DANLXQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.162"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1263)
                     /locus_tag="PXD94_002251"
     CDS             complement(<1..1263)
                     /locus_tag="PXD94_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL6287070.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMAGEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGH
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVA"
     gene            1429..5145
                     /gene="metH"
                     /locus_tag="PXD94_002252"
     CDS             1429..5145
                     /gene="metH"
                     /locus_tag="PXD94_002252"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL6287071.1"
                     /translation="MNTQHRTELLQNALTERILILDGAMGTMIQKYKLTESDFRGERF
                     KNSTIDLKGNNDLLTLTQPLLISAIHEKYLAAGADIIETNTFSATTIAQADYELESIA
                     YELNLVGAKLARLAADKFSIPEKPRFVAGILGPTNRTCSISPDVNDPGFRNITFMQLV
                     EAYAEATRGLIEGGADLIMIETIFDTLNAKAAVFAIETVFDELGVKLPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAKPLTFGLNCALGPKELRPYVEMMSKISETFVSVHPNAG
                     LPNAFGGYDLGAEEMATYIKEWAELGWLNIVGGCCGTTPEHIQAFAEATKGVAPRKLP
                     EIPTAMRLSGLEPLTIDDNSLFVNVGERNNVTGSAKFKKLIKEEKFAEAIEIAISQVE
                     NGAQVIDVNMDEALLDSKKCMTRFLNILATEPEAAKVPIMIDSSKWEVIEAGLQTVQG
                     KPIVNSISLKEGEEKFIHQAKLCRRYGAAVVVMAFDEVGQADTEERKVEICTRAYRIL
                     VDQLGFPPEDIIFDPNIFAIATGIEEHNNYGVDFIQACERIKRDLPHAKISGGVSNVS
                     FSFRGNNVVREAIHAVFLYYAIKAGMDMGIVNAGQLAIYDDLDPELREAIEDAVLNHR
                     HDTTDRLLEISEKYRGVKVESADESAAEWRNLPVAERLKHALVKGITTYIIEDTEEAR
                     QQFASPLEVIEGPLMAGMDVVGDLFGDGKMFLPQVVKSARVMKQSVAYLEPYINATKQ
                     KGSSNGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVIDLGVMVPADVIIETAIREKAD
                     LIGLSGLITPSLDEMEYFLGEMNRLKLDIPVLIGGATTSKEHTAIKLYPKYNYPVVYT
                     ANASRCVTVCATLMNPEAKAEFWEKTKAEYEEIQRKFAVRKPLRNGLSIEEARANRFD
                     GFSGEWADYVPPTPKQTGIIEYKNVPIATLRKFIDWSPFFMLWGLMGGYPDAFDYPEG
                     GEEARRVYNDAQRVLDEFEQNGKLNPSGIMGIFPAVREGDDIAILNEDRTACIGKSYH
                     LRQQSERGKNSKSPYNLCLSDFIADRESGQQDWFGMFAVCAGIEEHYLVESYKAAGDD
                     YNAILLQAVGDRLAEAMAEYLHFELRTKVWGYTVEDEDNGRLIREEYIGIRPAPGYPS
                     CPEHTEKQIIWDLLEVEQRIGMKLTESYAMWPAASVCGWYFTHPTSNYFTLGKIDEDQ
                     VLDYAKRKGWDELTAKKWLSVAMKQDVKSQAENI"
BASE COUNT         1552 a         1219 c         1289 g         1225 t
ORIGIN      
        1 agcaacctta aacaacatta gcattcccgg aataccaata aagaaacaga gccagaagaa
       61 ttggtaatag cccatcattt ccactaaatg ccctgtgtat gcccctaaca ttttgcttgg
      121 tagggcagca aggctggtga aaatggcgag ctgcattgca gtgtaagcgg gattagtttc
      181 acgagccata aaggcaacga aagccgctgt gcctaaaccc acgcccagat attcacctgc
      241 catcactaag cctaatttcc aaagctcggc agctccaatg ctttcggtag ggaaatgctc
      301 aaaactggca aggtaagcga agcctaaaat agtaattaat tgtacaaatc cgaacagcca
      361 taaggctcgg ttaacgccca atttcaacat tgccacgcca ccaataatac cgccgccaat
      421 gctgcaccaa agtgaagtta ttttcacaat gccggcaatg tgcgatttag taaaacccat
      481 atccaaaata aatttggttt gtagcgtagt tgcaagggaa tcgcctaatt tatataggaa
      541 aataaataga atcagcccaa tcgctcctgc cacgccttta cgggtgaaaa actctttgaa
      601 cggatcgaca aacgccatat aaaacggctt agaacggtcg attggcttat gattcggctc
      661 ggcagcgatt attagcgtaa cgaataagca aggcactaaa aaagcagcgg taatcagaaa
      721 tacggtatcc cacgcatagt gatcggctaa aaataatgaa agcccacccg gaatcagccc
      781 tgcaatacga taagcattaa cgtggataga gttacctaac ccgagttcgt tatccgataa
      841 aatttcacgc cgaaaggcat ccaccacaat gtcttgcgtg gcggaaagaa aagctaatag
      901 cacagaaaga gtaacaataa ttgaaatatc ttgcactggg ttgaacaagc caaagctggc
      961 taaaatcacc aatagcccga tttgtgaaat gaaaatccag cttttgcgtc tgcctaaaaa
     1021 aggtggaaag tagcgatcta acaatgcagc ccacaggaat ttccacgtgt agggcaaaga
     1081 ggtcagggtg aaggcggcaa tagttttaat atctaaattg gcactggtta gccaagccgg
     1141 cattagctga attaacacaa acaacggtaa accggaacaa aagccggtgt aaacacaaag
     1201 caacatattt ttagtaaaaa tctgttgcca gatagagcgt ttgggttcgt gacaagcggt
     1261 catatttttc ctacattttg caaaataaaa aggggaagaa ctgagccaat atagcataat
     1321 tttaaaggcg actctaaccc tttccatttc gtcacagata aggtttgaca gccttgcgac
     1381 atatttctat tattcctttt aatcaaacta aaaaactgga agcagagtat gaacacgcag
     1441 catagaaccg aattacttca aaatgccctt accgaacgca ttcttatctt agacggtgcg
     1501 atgggaacga tgatccaaaa atataaacta accgaatctg atttccgagg tgaacgtttt
     1561 aaaaacagta ccatcgatct taaaggcaat aacgacctac tcacactcac ccagccactc
     1621 ctgatttctg ctattcacga gaaatattta gccgccggtg cggatattat cgaaactaat
     1681 acctttagtg cgacgaccat tgctcaagca gattatgaac ttgaaagtat tgcttatgag
     1741 ctgaatcttg tgggggcaaa acttgcccgt cttgccgccg ataaattctc catccctgaa
     1801 aaacctcgtt ttgttgccgg tattcttggg ccgactaacc gtacttgttc gatttcccct
     1861 gatgttaatg accccggttt ccgtaacatt acctttatgc aactggtgga agcctatgcc
     1921 gaggcaaccc gtggtttgat tgaaggtggg gcggatctga ttatgatcga aaccattttc
     1981 gataccctga atgccaaagc ggcggtgttt gcaattgaaa cggtgtttga tgaactgggc
     2041 gtgaaattgc cgataatgat ctccggcacc attaccgatg cctcagggcg aacgctgtca
     2101 gggcagacca cggaggcgtt ttataactcg ctccgccacg ccaaaccgct gacgttcggc
     2161 ttgaactgtg cgctcgggcc gaaggaactc cgcccttatg tagagatgat gtcgaaaatc
     2221 agtgagactt ttgtttcggt tcacccgaat gccggcttgc caaacgcttt tggcgggtac
     2281 gatttagggg cggaagagat ggcgacttac atcaaagagt gggcagaact cggctggctg
     2341 aatattgtcg gcggctgttg tggcaccacg ccggagcata tccaagcctt tgccgaagca
     2401 accaaaggcg ttgcgcctcg taaattacct gaaattccaa ccgctatgcg tttgtcgggc
     2461 ttagaaccgc tgacgattga cgataattcg ctgtttgtga atgtgggcga acgcaataac
     2521 gtcaccggtt cggcaaaatt taagaagctg attaaagaag aaaaatttgc ggaagcgatt
     2581 gaaattgcca tcagccaagt ggaaaacggg gcgcaggtga ttgacgtgaa tatggacgag
     2641 gcgttgctcg attccaaaaa atgtatgacc cgtttcctca acattcttgc taccgaacct
     2701 gaggcggcaa aagtgccgat tatgattgac tcctcgaaat gggaggtgat cgaggcgggc
     2761 ttgcaaacgg tgcagggcaa gccgatagtg aactccattt cactcaaaga gggcgaagag
     2821 aaatttattc accaagccaa actctgccgc cgctatgggg ctgcggtggt ggtgatggca
     2881 tttgacgaag tcgggcaagc ggataccgaa gaacgcaagg tagaaatttg tacccgagct
     2941 tatcggattt tggttgatca gctcggcttc ccaccggaag acattatttt cgacccgaac
     3001 atttttgcga ttgccaccgg tattgaagaa cacaataact acggcgtgga ttttatccaa
     3061 gcctgtgagc ggattaaacg agacttgcca cacgccaaaa tttccggcgg ggtgtcgaat
     3121 gtctctttct ctttccgagg caacaatgtg gtgcgtgagg ctattcacgc cgtgttcctc
     3181 tactatgcga tcaaagccgg aatggatatg gggatcgtga acgcagggca gttggcgatt
     3241 tatgacgacc tcgaccctga actgcgagag gcgattgaag atgcggtgct gaaccaccgt
     3301 catgacacta ccgaccgcct gttggaaatt tccgaaaaat atcggggcgt aaaagtagaa
     3361 agtgccgatg aaagtgccgc cgagtggcga aatttacctg tggcagagcg tttaaaacac
     3421 gccctcgtaa aaggcataac gacctacatt attgaagata ccgaagaggc tcgccagcaa
     3481 tttgcctctc cgctggaagt gattgaaggg ccgctcatgg cgggtatgga cgtggtgggc
     3541 gacctgttcg gagacggcaa aatgttcctg ccgcaagtgg tgaaatcggc tcgggtaatg
     3601 aaacagtccg ttgcctattt agagccgtac atcaacgcca ccaagcagaa aggatcgagc
     3661 aacggcaaaa tggtgatcgc caccgtgaaa ggtgatgtac acgacatcgg caaaaacatc
     3721 gttagcgtgg tgttgcagtg caataacttt gaagtgatag acttaggcgt gatggtgcct
     3781 gccgatgtaa ttatcgaaac tgctattcgg gagaaagccg atctcattgg gttaagcgga
     3841 ctaattaccc catcgctgga cgaaatggag tattttttag gtgaaatgaa ccgcttgaag
     3901 ctcgatattc cggtgctgat tggcggcgca acgacctcaa aagagcatac ggcgatcaaa
     3961 ctttatccga aatataacta ccccgtagtc tataccgcca acgcttcacg ttgcgtaacc
     4021 gtctgtgcga ccttgatgaa ccctgaggca aaagccgaat tttgggaaaa aaccaaagcc
     4081 gaatacgaag aaattcagcg taaatttgcc gttcgcaaac cacttcgcaa cggtttatct
     4141 attgaggagg ctcgagccaa tcgttttgac ggatttagtg gcgaatgggc agactatgtg
     4201 ccgccaacgc caaaacagac cggcattatc gagtacaaaa atgtaccgat tgccacccta
     4261 cgcaaattta tcgactggtc gccgttcttt atgctctggg gcttaatggg cggttatcct
     4321 gatgcctttg attatccgga aggcggcgaa gaggcacgcc gtgtctataa cgatgcccaa
     4381 cgggtgctgg acgaatttga gcaaaacggc aagctcaacc caagtgggat tatgggcatt
     4441 ttccctgccg tgcgtgaggg cgatgatatt gcaattttaa acgaagatcg caccgcttgt
     4501 atcggcaaat cctaccacct ccgccaacaa tccgaacgag gcaaaaacag caaaagcccg
     4561 tacaacctct gtttaagcga ttttatcgcc gaccgagaaa gcggacaaca agactggttc
     4621 ggaatgttcg ccgtttgtgc cggcattgaa gaacactatt tagttgaaag ctacaaagcg
     4681 gcaggcgatg actacaacgc cattttgctc caagccgttg gcgaccgctt ggcagaagca
     4741 atggcggaat acctacactt tgagctacgt accaaagtct ggggctacac cgtggaagac
     4801 gaagacaacg gacgcttaat ccgtgaagaa tacatcggca tccgccctgc gcccggctac
     4861 ccaagctgcc cggaacacac cgagaaacag ataatttggg acttgttaga ggtggaacaa
     4921 cgcatcggca tgaaactcac cgaaagctat gccatgtggc cggcagcaag tgtctgcggc
     4981 tggtacttca cccaccctac cagcaactac ttcactctcg gcaagattga tgaagatcag
     5041 gtgttggatt acgccaaacg caaaggctgg gacgaactca cggcgaagaa atggttaagt
     5101 gtggcgatga aacaagatgt caagtcgcag gctgaaaaca tttaatgtag tggcgggttt
     5161 taacccgcct gttacaagcg gttggttttg agataaaata tgcaaaattt atctctcttt
     5221 tatgcaaaga agttatcatc gagaacaaaa caatgattga aaaacaaaat tatcacttag
     5281 tacaa
//
LOCUS       DANLXQ010000163         5284 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.163, whole genome shotgun sequence.
ACCESSION   DANLXQ010000163 DANLXQ010000000
VERSION     DANLXQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.163"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1040)
                     /locus_tag="PXD94_002253"
     CDS             complement(<1..1040)
                     /locus_tag="PXD94_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL6287072.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIATMLVVPYL
                     ILVNYLFFYGKN"
     gene            complement(1050..2234)
                     /gene="neuC"
                     /locus_tag="PXD94_002254"
     CDS             complement(1050..2234)
                     /gene="neuC"
                     /locus_tag="PXD94_002254"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL6287073.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIEQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRDESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            complement(2235..3494)
                     /locus_tag="PXD94_002255"
     CDS             complement(2235..3494)
                     /locus_tag="PXD94_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL6287074.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLFSHYPIEFIKRAEHLASDKASSFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDECLKHFQLDY
                     SNYARQLHHPEYSPNGAIFTAKPEAYLKQKHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPKDIILMFGTNDIVYAGWSDEKILADINHLIAQL
                     TAIRGDINLYFLEITPTAFRVDRNNNDIRLLNQYLKDNLAVKWISLDQAFSDKYGKLD
                     LSYSDDGLHLNAKGYEKLTAILEKELN"
     gene            complement(3554..4591)
                     /gene="neuB"
                     /locus_tag="PXD94_002256"
     CDS             complement(3554..4591)
                     /gene="neuB"
                     /locus_tag="PXD94_002256"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL6287075.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            complement(4591..5235)
                     /locus_tag="PXD94_002257"
     CDS             complement(4591..5235)
                     /locus_tag="PXD94_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL6287076.1"
                     /translation="MQQKSKREKLILIGAGGYAKSVLDSLDHEQYEFCGLIDNFKPEG
                     SVHLGYPILASTIDCFSKRDNYHYFICIGNNSYRLEKFSKLQKYKCRIINVVDKTALV
                     SKNSTLGIGVFVGKMAIVNSGVTVGDNVIINTKSLVEHGCFIGSHCNISTNTTLNGDV
                     IVEDHAFIGSSSVVNGQLRVGESALVGSGAVVIRNVEPRTVVAGVPAKYIKDIK"
BASE COUNT         1653 a         1111 c          870 g         1650 t
ORIGIN      
        1 ttatttttgc cataaaaaaa caaataattg actaaaatga ggtaaggcac aaccaacatt
       61 gtcgcaatta aaaagagaat aatgtagtat tttgttccta caaattggct acccagaatc
      121 cataccacta attcttccgg aataaaccac atcacaatag caggtaatgg tacaataaat
      181 aaggcataca acgcccattt gtggatatgg ctgacattaa tttttttctg ctttaaggct
      241 tcataaaaat aaggcagtgt tgctttattt aaggcttgaa ccacaatggc tagaatcatc
      301 gcaatttgag cccccattgc atataaacct aaatcctctt cgctaaattt atggtaaata
      361 aatactctgt ctaactgtcc ttttaagaaa atactggaat ggtgcaaaat caacggaata
      421 ccaaagccga ataaatacaa taaagcagtt ttatggcggg caaggctaaa tttcttcgtt
      481 tttgctcttt tagcatacaa aaagtaagcg attagaaaga ctaagccatt actcatcaaa
      541 atcgctaaaa tccgcttttc cactaaatcg gtcttaaaat actccatcat tgcaacggta
      601 ataactgccg aaaaaatcgc cgacattaat tgaatcgttg tataagcaag tgcctgtttt
      661 tgacactgcc ttacactaag ctgtacattc aaaaacgact ggaacatcgc cccaatcgct
      721 acataagcaa taatttcaga ctggaaatac cagcaaccca acaggataag caaagttgca
      781 caaagagtat ataaataccc tgtactcaag actaaattta gtgaacgctt accataaaaa
      841 tagaaatagc gagcaaccgc accgtcttgg ctaagcccga taaaaattac aaataaagca
      901 gcaaaggttt ggtagtagga aagctcccca taacctgcca cgccgagttt gcgtgaaaag
      961 tagggtaaga gcaagaaagg aattgacttt gacgccaatt ccccaattaa ataaatcaca
     1021 ctgtctttaa ctgctttcat agttgcaaat tattctgatt ttttgaccgc ttgcgagaaa
     1081 gcaacatcat aaaatccttt cggttgagta tggtggctgt ctgctaagaa tattttgatt
     1141 gcattgtaag ccttttccat cgcgttgtct tgaaaatatg gatttttcat actcggtaaa
     1201 cggtgttgga aatcggctga aatcaatttt tcgataccat ctatgatagc ctgttcatta
     1261 ctctctacat caattacgct ctcatcccgt actcgccctt gctggcgatt accgatattt
     1321 agcgtaccaa caccacaact cggtgcttca atcaagcctg atgagctatt accaatcaac
     1381 ccatttgaat atttaatcaa cgctaaatac tcttccggtt tgactgaggt gaaaaactta
     1441 aattgatttt catcggtgta agccttaaaa cgtgcataaa tttgctctga attggtgtct
     1501 gaatttgagc cgataaagac gaattggtat ttgtctttaa actgctcaaa agcattgagt
     1561 aacgcctcaa tttgctccaa cacggaaaca tcggttaagg tttccgggtg gaagaccact
     1621 aaaaaataag gcgatgccgg aatccctaat ctctcgacta attctgcttg ggtcggcaac
     1681 actaaatcca aactattctc cgcccctaga gaaccagtat tcaccacatt ttcgggcagc
     1741 tcgcccattt gaatcacacg ctgtctatat tcttcagttg aagttaaatg caatttcgac
     1801 attttggtaa tacaatggcg aataaattca tcgtaattac ctaatgtttg ctcaccaccg
     1861 tgcagatgaa tcagcggaat attatgcatc gccgcagccg ttgccaccgc caacatctcg
     1921 taacgatcgc ctagcacaat caccgcatca taacgatgtt gctggaaatg ttcgccaaat
     1981 cgatctaaac actctgccat tgaatgaata atggtctgat tattttcact gtttaaggta
     2041 agcggaatac gagctgcaat ctcaaagcca tcttgctcaa tgaccgtcac cgtattgcca
     2101 tattgcggat ccaaatgcat cgcagtaaca accagagaga aattcacagt cggatcttgt
     2161 gctaattttt gtaataaacg ctttacaatc ccgtattcgg cacgagatcc ggtaatatag
     2221 gcaatatttt tcatttagtt gagctctttt tccaaaatag cggttaattt ttcataacct
     2281 tttgcattta aatgtaatcc gtcatcgcta tagcttaaat ccaatttgcc atatttatcg
     2341 ctaaatgcct gatctaagct aatccatttc accgctaaat tatctttaag atattgattt
     2401 aacaagcgaa tatcgttgtt attacgatcg actctaaatg cagttggggt aatttccaag
     2461 aaatagagat taatatctcc acgaattgct gttaattgtg caattaaatg attaatatct
     2521 gctagaattt tttcatcact ccagccagca tatacaatat cattagtacc gaacattaaa
     2581 ataatgtctt taggtaaagc agaaattaaa ccttcatcca aaatcagatc actgtattgt
     2641 tttgttgaaa tacctgcaat tcctaaatta ttaactgcct taccgtttaa atgttcaata
     2701 tcccaatatt caatgatcga atgcccgatt aaggctatct ctttcacttc tttaaagttc
     2761 tcttttttca ttaaaatcga acgtttaatg gtttcaagta agattttttc tttattgcgt
     2821 tgttgcagaa taaagtagaa gtactcgaaa tcttggcgat catcaatatc aatagaaacg
     2881 tctttatcca taaaataggc tagacatttc gcaccataga agtgtttttg tttaagatag
     2941 gcttctggct tagctgtaaa aatagctcca ttcggtgaat attcaggatg atgcaattgg
     3001 cgtgcatagt tagaataatc caactgaaaa tgctttaggc actcgtcttc ctcaatcata
     3061 cgagtcaatg tcgtaggttt atgggcatca gaaacagata ctaagaaatc aaattgttcg
     3121 aaatttttct caaatttttc acacgattcc ttaatatgct ctcctgatct caacggcgaa
     3181 gttggttgaa gtagcataaa atagtcaaac tctactgatc tatattgatt taacacatct
     3241 tcaatcacga caaaagaact cgccttatca ctggctaaat gctcggcacg tttaataaat
     3301 tcaattggat aatgtgagaa caaatcaatg tattcttggg aatcagtact aacgataatt
     3361 ttctcaaatt gccccgactc aatcgcagct tcaatagaat aggccattaa cggcttaccg
     3421 ttggctaata atacattttt attcggcaag ccttttgaac cggctcgggc tgtaataatt
     3481 gcaatttttt tcataaattt gaccgcttgt taaacacaaa atgatggagc tttcatccca
     3541 tcactatatt atattacatc tgattttcaa aacgagaatc ttgaattaat tgatcttctt
     3601 caaaatcctg ttgtgcttct ttacctagca catcatacca aaacatcggg ctaatgccat
     3661 taccggggcg tttagtcgta atattttcgg ttgtaaaaat ttcgcctttt ttaattggac
     3721 gtgccgcaat aattgatttt cttgccacaa ttttattttt tgcttccgaa ttagtgacta
     3781 atttctcgga agaacccagc gatttttcca ccgcacgaat gccttcacac agtaatttca
     3841 gctcttcagg tgtaaccgaa gctttatgat ccggaccttc aaagttttta tctaaggtaa
     3901 aatgtttttc aatgaaggta atgccgtaag gtactgccgc caaccctgca aaataaccgg
     3961 cagagtggtc agaaaaacct aaactatagt cgccaaactc ctgttttagc tgatggaacg
     4021 catttaaatt cacatcttca taaggtgtcg ggtactcggt attacaatgt aaaatcgtaa
     4081 tatctttttt cgctacaccg ttatcttcca atacttttaa tgaggctttt gtttcttcaa
     4141 tggttgccat accggtagaa ataacgatcg tcttgccttc aatcggaagt cgtgcaattt
     4201 tttctaaata cggcaaattg gttaattcac cagacggaat tttccacact ttttgttgtt
     4261 ggcttgccaa gaaatcaatc gactcgaaat cgaatggcgt agagaatacc tctaacccca
     4321 atgaacgagc gtaagcctct aatttcacaa actcctcata aggtaactct aatttgcggg
     4381 tcatttctaa ttgggaatca gcggtgccgg tcgttacttt ttgatactcg gctttagggg
     4441 catatttaga gatcaactta tccgctttaa aggtttggaa tttcaccgca tcgacaccac
     4501 actctttcgc tacatcaacc attttctttg ctaacgcagg atcgccgtta tggttacaac
     4561 cgatttctgc cacaataaat acatttacca ttattttata tccttaatat attttgctgg
     4621 aacgcctgct acaactgtgc gaggctccac atttctaatt accactgcac cggagccaac
     4681 caatgcactt tcacccactc ttagctgtcc gttcacaaca gaggaactac caataaaagc
     4741 atgatcttcg acaattacat cgccatttag tgtcgtattg gttgaaatat tgcaatgact
     4801 gccgataaaa caaccatgct caaccagcga tttagtgtta atgatcacat tatcgcctac
     4861 agttacgcca ctgtttacaa ttgccatttt tccaacaaac actccaatgc ctagagtgga
     4921 atttttggac accaatgcgg ttttatctac cacattaatt attctacact tatatttttg
     4981 tagcttagag aatttttcta aacggtaact attatttcca atacaaataa aatagtgata
     5041 attatctcgt ttagaaaaac aatcaattgt tgaagctaga ataggataac ctaagtgcac
     5101 actaccttca ggcttaaagt tatcgataag tccacaaaat tcatattgtt cgtgatcaag
     5161 tgaatctaac actgatttgg catagccacc ggcaccgatc aaaatcaatt tttctctttt
     5221 agacttttgt tgcattgcta tcacccatga tgaatttcaa ttcatatgga gtattctaat
     5281 actt
//
LOCUS       DANLXQ010000164         5226 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.164, whole genome shotgun sequence.
ACCESSION   DANLXQ010000164 DANLXQ010000000
VERSION     DANLXQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.164"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..623)
                     /locus_tag="PXD94_002258"
     CDS             complement(<1..623)
                     /locus_tag="PXD94_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL6287077.1"
                     /translation="MKAQIELPPKLLPVFAKDYRYKGAYGGRGSGKTRAFAKMSAVSA
                     YKRAMRGESGVILCGREFMNSLEESSLEEVKQAIKSEPFLANFFDVGEKYVRTKDGRI
                     SYVFSGLRHNLDSIKSKARILIAWIDEAETVSETAWRKLIPTVRENNSEIWVTWNPEN
                     RGSATDTRFRQNPPENSCIIEMSYQDNPWFPDVLEQERLNDKQRLDDA"
     gene            complement(607..1131)
                     /locus_tag="PXD94_002259"
     CDS             complement(607..1131)
                     /locus_tag="PXD94_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL6287078.1"
                     /translation="MTKKGEVKATSKGVGKLTDKQKRFVEEYLIDLNATQAAIRAGYS
                     EKTAYSIGEENLRKPEIRSAIQEAQNKRSERTQITQDDVLNGLLEVIAMSTGKKIVTE
                     TDVAKNDNGELVGFDIAKTKFEPAAANKALELLGKHLGMFKDKVDLINSDGSLNRPMV
                     IELIAPSLDDESTD"
     gene            complement(1523..1696)
                     /locus_tag="PXD94_002260"
     CDS             complement(1523..1696)
                     /locus_tag="PXD94_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287079.1"
                     /translation="MYPPAAYLVPCGRTEFSGRTYGDTVEYLVKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            complement(1701..2051)
                     /locus_tag="PXD94_002261"
     CDS             complement(1701..2051)
                     /locus_tag="PXD94_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL6287080.1"
                     /translation="MLNWISGTIAIVILGLCVWLWGQSQMIDSLRAENRAQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLHQQNNHKH"
     gene            complement(2024..2593)
                     /locus_tag="PXD94_002262"
     CDS             complement(2024..2593)
                     /locus_tag="PXD94_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL6287081.1"
                     /translation="MSKSLKYGSGIVCGIAAIITLVQYQHPEIRTNQAGLEIIGNAEG
                     CRRDPYKCPADVITVGIGSTEFGGEKIDPNRIYSDKEIAERWAKDLKIAESCVNRHFN
                     GKDMNDNQFSGMTSAVFNMGCYNMRFYRNKQGQYVQTTIHKLAVNKQFEEMCHRLPDF
                     IRASGKVLNGLVIRREKEKALCLTGLVAR"
     gene            complement(2586..2831)
                     /locus_tag="PXD94_002263"
     CDS             complement(2586..2831)
                     /locus_tag="PXD94_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287082.1"
                     /translation="MKLMKDMGTQSYIWSGFSTWLAWLGEQQNLMLLSLAIGIVTAIA
                     GLIQRFDEWRRRKREDERAEELHQAKMARLRKGLSDE"
     gene            complement(2974..3144)
                     /locus_tag="PXD94_002264"
     CDS             complement(2974..3144)
                     /locus_tag="PXD94_002264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287083.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
     gene            complement(3170..3355)
                     /locus_tag="PXD94_002265"
     CDS             complement(3170..3355)
                     /locus_tag="PXD94_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287084.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            complement(3372..3447)
                     /locus_tag="PXD94_002266"
     tRNA            complement(3372..3447)
                     /locus_tag="PXD94_002266"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3412..3414),aa:Trp,seq:cca)
     gene            complement(3675..4148)
                     /locus_tag="PXD94_002267"
     CDS             complement(3675..4148)
                     /locus_tag="PXD94_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287085.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWQEIIQDKCKKAERIIADILESKISSSNSTDKLKKYQFSP"
     gene            complement(4138..4707)
                     /locus_tag="PXD94_002268"
     CDS             complement(4138..4707)
                     /locus_tag="PXD94_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL6287086.1"
                     /translation="MANKPPKQHKCKECGSYYIKFQSTQQVCSVKCAMAMGKRKTEAK
                     RKKSAKIDRLEQRKRLEKLKSRRDFEKDLQKIFNKFIRLRDKDLPCISCGRYHQGKYD
                     AGHYKTVGGNPELRFNEDNCHSQCVPCNRHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            complement(4835..5029)
                     /locus_tag="PXD94_002269"
     CDS             complement(4835..5029)
                     /locus_tag="PXD94_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287087.1"
                     /translation="MTKFFETIAFVMLFGIIPSCLLAINTTLMNMNIKNAPWFMVIIQ
                     LGFNITCYWLWFISVMRNVI"
BASE COUNT         1471 a         1251 c          855 g         1649 t
ORIGIN      
        1 gcatcatcaa gccgctgttt gtcatttagc cgctcttgct cgagcacatc aggaaaccaa
       61 ggattatctt ggtagctcat ttcaattatg cagctgtttt caggtggatt ttgcctgaag
      121 cgagtatctg ttgcgctccc tctattttcc gggttccaag ttacccatat ttcggaatta
      181 ttctcacgca cggttggaat taatttccgc caagcagttt cgcttactgt ttctgcttca
      241 tctatccacg caatcaaaat acgggcttta gatttgatgc tatctaagtt atgccgaagt
      301 ccggaaaaca cataagaaat ccgcccatct ttagtgcgaa catacttctc gccgacatca
      361 aaaaaatttg ctaaaaaagg ctcagacttg atcgcctgtt taacttcttc aagagagctt
      421 tcttccaatg agttcataaa ctcacgaccg caaagaataa cgccactttc ccctcgcatt
      481 gctcttttat aagcagacac tgccgacatt ttggcaaatg cccgagtctt gcctgaacct
      541 cgcccaccat aagcaccttt gtaacgataa tctttggcaa aaacaggcaa cagtttaggt
      601 ggtagttcaa tctgtgcttt catcgtctaa actcggagca attaattcaa tcaccatcgg
      661 tcgattcagt gaaccatcag aattaattaa atcaaccttg tctttaaaca ttcctaaatg
      721 tttaccgagt aactcaagag ctttatttgc ggcggcaggt tcgaattttg tttttgcaat
      781 atcaaaaccg accaactcac cattgtcatt ttttgcaaca tcagtttctg tcacaatttt
      841 tttaccagta ctcattgcaa tcacttcaag caacccattt agcacatcat cttgtgtaat
      901 ttgggttctc tccgagcgtt tattttgagc ctcctgaatt gcacttctga tttcaggttt
      961 tctcaggttc tcttctccga ttgagtatgc tgttttttcg ctgtacccag ctctaatcgc
     1021 agcttgagtc gcattcaagt caatcagata ctcttctaca aatcgtttct gtttatcggt
     1081 taatttaccc acgcctttag acgtggcttt aacctcgcct tttttagtca ttgggttaaa
     1141 ttcctttact tcattgcatt taatagatta gcaatagcat taatcagctt tggcgacacg
     1201 aacgccaaaa tagggactgt tactgctagg cttatttgcc ataaaccata ttccataaga
     1261 atctccttga tggttacggt taaaaatgta ataatttcca taacttgtta ctcattccgt
     1321 ttcaatgggt aataaaaaaa cctcgaacac cgcaaatgtt cggggttttg ttttatataa
     1381 atcacatcac ttaggtattt cctcggtgtt ttatccaacc aaccccgaaa ccatagctaa
     1441 attatgtaat atttactttc catcaaaaca aaaagggagc tgttacgctc ccattttcaa
     1501 tagcggttaa ttagataatt agctatttaa acccttgttt ggtttgtgct tgccattctc
     1561 tgatacggtc aatttgatta gcacacaaat cacgctctcc catcactttg acgagatact
     1621 caaccgtatc gccataggtt ctaccgctaa attctgttcg cccacacggc acaaggtaag
     1681 ctgccggcgg atacaaatac tcagtgcttg tgattgttct gctggtgcag ccgcttaatg
     1741 ctatcagcaa cgccattagg caaatcagct ttagcacaac tgtcttgtgc cagtattgat
     1801 gtaatttcat tcttggccat ctccacttta ttccgcagct catttgcaat tttttggatt
     1861 ttttcgaccg cttgtcgctc ttgctctaag ctatcggtta gccgttgatt ggctttttgc
     1921 tgctgctcaa tggtttgggc ttgtgcgcgg ttctcagctc ttaagctatc tatcatctgt
     1981 gactgacccc ataaccatac acacaagccc aaaatcacaa tggctatcgt gccactaatc
     2041 cagttaagca taatgctttc tccttttcac ggcggataac caatccgttc aataccttac
     2101 cactggcacg aataaagtca ggtagtcgat gacacatctc ctcaaattgt ttattcactg
     2161 cgagtttatg gatggtagtc tgcacatatt gaccttgttt attccgataa aatctcatgt
     2221 tataacaacc catattaaat acagcggacg tcattcccga aaattgatta tcgttcatgt
     2281 ctttaccatt aaaatgacga ttgacacaac tctccgcaat cttcaaatct tttgcccaac
     2341 gctcagcaat ttctttgtca gaataaatac gatttgggtc aatcttttca ccgccaaact
     2401 ccgttgagcc aatgccaacc gtgattacat cggcagggca tttatacgga tcacgtctgc
     2461 aaccctcagc attaccgata atctctaatc cagcttggtt agtgcggatt tcgggatgtt
     2521 gatattggac taatgtaata atggcagcga taccacaaac gataccactg ccatatttaa
     2581 ggcttttact catcacttaa cccctttctc aatcttgcca ttttcgcttg atgaagctct
     2641 tccgctcgct catcttcacg cttacgccta cgccattcat caaatcgctg aataagccct
     2701 gcaatggcag taacaatacc gatagcaaga ctaagtaaca ttaaattctg ctgttcgccg
     2761 agccacgcca gccaagtgct aaatcccgac caaatatagc tctgcgttcc catatctttc
     2821 attaacttca tactccaccc cgtttcgagg caataaaaaa gcccagtcgt gagactgagc
     2881 tttggttaaa aattctgcta gaatactgtt ccccaacaaa taaactagca gagggtaaaa
     2941 atgattgaat ttactctgta ccaccagaag catttaacct tgcttcacgt cattattact
     3001 ggcaaatgga taaattttcc tacttcggaa gtgctgaacg caatctgata tacgtcgaga
     3061 tggccagccc acggcatagc gatcttgaga tttttcttga tcttcttcac cgctttttga
     3121 ctgagtattt agttgttccg tcatagcaat ccctcattga tttttgtagc taagatagat
     3181 cttcaaccct tgggaaaggg aaatttggcg gagccttaat tcctaacttt tcatctctta
     3241 atcgagagag ggctttgcaa ccagcaaaag taagtgttcc agcttcagaa aaataaggct
     3301 tatcttcacc aaatttttct ctgcattctt gaattgcatc atcaaggtta tacataacat
     3361 ttactcgcat ttggaaaggg tggttggact caaaccaaca accaacgatt ttggagaccg
     3421 ctgctctacc aactgagcta cacccttaaa atccgtaaac aaaaaagccc caagcatttc
     3481 tgctcagggc tgttaaacta attcggtcgg catactgcca cgcaaacgct gaaataacta
     3541 tactcgacgt cgtttctagt tattctcctc cgaactgaat attacttcag gctgcgtctt
     3601 taactaacat tggtaaacat catttatact acgtccacca ttagcacaaa atatacactt
     3661 gttacgcact aaaatcaagg ggaaaattga tattttttca gtttatctgt agaattactg
     3721 gatgaaatct tgctttcaag aatatctgca ataattcttt ccgctttttt gcatttatct
     3781 tgaataattt cttgccatcg tctttctttt ctaccctcgc tgtcccttag ttgataataa
     3841 atagcgattt gccgctccga acgcccgaac cagtattttt gtttgagtat atcggctaac
     3901 attacgtctc ttttggtgac taccgtaaaa atctcattga tcagcgtgcc gaggctatcg
     3961 ttacaaacct ctctagatga aagactttta accccctccg cagatttcat cagccggtaa
     4021 atcatattta cccgactttc taaatccaat ccgccaaatt cccacgcccc ccataacctt
     4081 agcaactctt cgatccattt ctgcttttca ggctctaacc acttgttttt aatttttcta
     4141 ggtaacacgc tcaagctcct tgcacttcgc tttatagact ttgattaaat ctttgatttg
     4201 ttcgatagta agttttaatg gtgggtggtc tttacgctct aaaaattcca cacgctcaag
     4261 cccgattttc tcaatcagat ttactcgata attcacaata ttgccgtgca gatgtcggtt
     4321 acacggcaca cattgactgt ggcaattatc ttcattaaaa cgtagctcag gattaccacc
     4381 aacggtttta taatgccctg catcatattt cccctgatga tagcggccac aggaaataca
     4441 gggtaaatct ttatcacgca agcgaatgaa tttattaaat atcttttgta aatctttctc
     4501 aaaatctcta cggcttttca atttttccag ccgcttccgt tgctccaagc ggtcgatttt
     4561 tgcacttttt ttgcgtttcg cctctgtctt acgcttaccc attgccatag cacacttaac
     4621 agagcagacc tgctgcgtgc tttgaaattt gatgtaataa ctgccgcact ctttgcattt
     4681 gtgttgttta ggtggtttgt tagccattcc aaccctcacc atctctctta tcaaaccact
     4741 caataaaaaa gagtgaaccg gcacatacac taagaatagc gatggcgatt aaaattacag
     4801 tcagttcatt gcactggctc acttattaat ctccttaaat aacattccgc ataacagaaa
     4861 taaaccacaa ccaataacaa gtgatattaa aacctaactg aataatcacc ataaaccacg
     4921 gtgcgttttt aatattcata ttcattaaag tagtattaat cgccaacaaa cagcttggaa
     4981 taatgccaaa taacatcacg aatgcgatag tttcaaaaaa ttttgtcatc ttacttctcc
     5041 ttcccccgaa ttcgagggaa ttaataaaca cacactaaat cggagcaaaa ccgcgcttaa
     5101 aataataaag ccattcttga tatgtccatt gacagaaaac aataaaacta ccgactgctg
     5161 caaagaatgc taatggataa atcaataaat aacctacata tctaacagta agaatgacta
     5221 agaaaa
//
LOCUS       DANLXQ010000165         5196 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.165, whole genome shotgun sequence.
ACCESSION   DANLXQ010000165 DANLXQ010000000
VERSION     DANLXQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.165"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..202)
                     /locus_tag="PXD94_002270"
     CDS             complement(<1..202)
                     /locus_tag="PXD94_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287088.1"
                     /translation="MSLLKKAQTFISIFNEVRAKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGI"
     gene            complement(323..640)
                     /locus_tag="PXD94_002271"
     CDS             complement(323..640)
                     /locus_tag="PXD94_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6287089.1"
                     /translation="MLSRKEVLMTRRASMNLQQIIRNVVEYTYSYESGDKLSAEIHNT
                     LDNLSYFPRIGVIGSVENTRECFCRGYRIIYRETENQVQILAIIHCRQQYPTDYYRSA
                     FKA"
     gene            complement(633..791)
                     /locus_tag="PXD94_002272"
     CDS             complement(633..791)
                     /locus_tag="PXD94_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287090.1"
                     /translation="MTEEYQNFLIEQVKQGLDDIENGRVISLEESRQLCQKTIYETLQ
                     ELQGAVYA"
     gene            complement(845..1000)
                     /locus_tag="PXD94_002273"
     CDS             complement(845..1000)
                     /locus_tag="PXD94_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287091.1"
                     /translation="MLFKEKGYDEFLTQKIAKGRAELESGKGIPLEQATLLVQSTIEE
                     TAQELDD"
     gene            complement(1138..1395)
                     /locus_tag="PXD94_002274"
     CDS             complement(1138..1395)
                     /locus_tag="PXD94_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287092.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFYNYQQFMPEVEAKESLR
                     EQEPELDDGYNEEEYSEEEYSDENDASDENDNYDETYDDNF"
     gene            1409..1585
                     /locus_tag="PXD94_002275"
     CDS             1409..1585
                     /locus_tag="PXD94_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287093.1"
                     /translation="MAYVRIYQGEAYGWCAEKGEANTECPEALLVSLTGDIFEAVGGD
                     KLKGAKKWQPYKKE"
     gene            1589..1735
                     /locus_tag="PXD94_002276"
     CDS             1589..1735
                     /locus_tag="PXD94_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287094.1"
                     /translation="MDTTQKRVALFIIGLSVVMWLRKHNCCTVIFVLLLAALAFSYTM
                     EHLY"
     gene            1751..1957
                     /locus_tag="PXD94_002277"
     CDS             1751..1957
                     /locus_tag="PXD94_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287095.1"
                     /translation="MVKNVVHEEILHNRAELQQAVEKEEFCAKCDNSVVFHLRDNYHE
                     FTLGLDTILQCLHFAEEKGAIRIP"
     gene            1954..2730
                     /locus_tag="PXD94_002278"
     CDS             1954..2730
                     /locus_tag="PXD94_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287096.1"
                     /translation="MNIEYSLNHCQGDGVAFYGRLGTDDLIQLFNHLNPQKRKQKMFA
                     NLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMELYAKTADQLVFFNDSDARRNWYF
                     PTSKVYKYKGLWDEFIQDLEQYIKGTSREMESVGYQIIEASPYQTETVFKFNTENYLV
                     ELKRKPSEFYSELVDWAFGDMCDFEATIEQICQGQLCYADFEATVTDKKSEIQLGLAR
                     LSGISYSPNDRSFSGYRQELIGEAIASARHNAARFAYLHA"
     gene            2845..3257
                     /locus_tag="PXD94_002279"
                     /pseudo
     CDS             2845..3257
                     /locus_tag="PXD94_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
     gene            3271..3426
                     /locus_tag="PXD94_002280"
     CDS             3271..3426
                     /locus_tag="PXD94_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287097.1"
                     /translation="METRIVKVIYDDGDYFFTRINADEQAIRNYYAIGSSIGFDDIRK
                     IIAVEIL"
     gene            3506..4480
                     /locus_tag="PXD94_002281"
     CDS             3506..4480
                     /locus_tag="PXD94_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL6287098.1"
                     /translation="MNTQPKFDLYQHITDRIVVALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRPDE
                     REKLDEEGNIVYDEEGNPEMERHFFINKHNLFNLDQCENLPVELYDPVSEPKQPKSEP
                     NQYDIFAEIRQIIKGMDLQVEMKPSNRAFYQPGEDKVVMPEMKQFANAQAFHSVLLHE
                     MTHATGANNRLNREGITSGKAKFGNKVYAFEELIAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVLKEDKRAIVRASGKAREACQYMLDALNVAQLADKYDVFEEAA"
     gene            4567..4764
                     /locus_tag="PXD94_002282"
     CDS             4567..4764
                     /locus_tag="PXD94_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287099.1"
                     /translation="MTKSTEIPRGHDNWVITGRDNDGKWHKERFLYYSRREAIQQFRR
                     KHNCVGKHSVIESITPQFGWY"
     gene            4841..5083
                     /locus_tag="PXD94_002283"
     CDS             4841..5083
                     /locus_tag="PXD94_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287100.1"
                     /translation="MFNLNLFKRPIELSTLESWSKFCDDVGKVAFIAIPPMIYSNYDL
                     CIKIGNISALLAITYGFLLSGRTIRQFIEKEKSRWD"
BASE COUNT         1553 a          913 c         1085 g         1645 t
ORIGIN      
        1 gaataccaca accataatgg tcagcaaagt aagtagaacg cttttgaggc ttaagggaga
       61 acccattcat ctcagctctc atttcagcag catttttcaa ggcaacattt aaattgtttg
      121 aagactggcg ttgagcttca tttttagctc gcacttcatt aaaaatagag ataaacgttt
      181 gtgctttttt aagtagagac attgtggttc tcctgttttt tgcacctaat attattgaac
      241 tatggtaggc ttggcgagca ttgccttgag ttttaattcg gctacggtca tttttctttt
      301 tatttccatt ttatagctcc tgttatgctt tgaatgcaga gcgataatag tcggttgggt
      361 attgttgacg acaatggata atggctaaaa tttgcacttg attttctgtt tctcgatata
      421 taattctata acctcggcaa aagcattctc tagtattttc tactgaacct ataacaccaa
      481 ttcgtggaaa ataagaaaga ttatccaaag tgttatgaat ttccgcagat aatttatcac
      541 cactttcata gctataagta tattccacaa cattacgaat gatttgttgt aaattcatac
      601 ttgctctgcg agtcattaac acctcttttc gactaagcat acacggctcc ctgtagctct
      661 tgcaaggttt catatatggt tttttgacac agttgacggc tttcttctaa tgaaataaca
      721 cgtccgtttt ctatatcatc aagtccttgt tttacttgct ctattaagaa gttttggtat
      781 tcttctgtca tggataactc ctatcattag ttgtatatag atatattagc aatgttacaa
      841 attgttaatc gtccagttct tgagcagttt cttcaatagt tgattgaact aacaatgtgg
      901 cttgctcaag aggaatacct tttccggatt ctaattcagc tctgcctttt gcaatttttt
      961 gagtaaggaa ctcatcgtag cctttttctt taaatagcat tttatgttcc tttcacttat
     1021 tacaaggctt tcaggcatag ggattatgtt ctcctaaatg cgaaatattg gaataattta
     1081 cgcttaaagc aatagagtgt caagagcagg gtttttggaa gtgtggtttg ggaatattta
     1141 aaaattatca tcataagttt catcgtagtt atcgttttca tcgctggcat cattttcatc
     1201 ggaatattct tcttcgctat attcctcttc gttatatcca tcatcaagtt caggttcttg
     1261 ttcccttagt gattctttag cttcaacttc aggcataaat tgctggtaat tgtagaaaaa
     1321 agtgccatcg ctgccttttt cccaaagtcc ggcgtagttg tattttacat attcaggatc
     1381 agcgtggcat atcatcccta attgaggggt ggcatatgtt cgtatttacc aaggtgaggc
     1441 ttatggttgg tgtgctgaaa aaggagaggc aaatacagaa tgccctgagg ccttattggt
     1501 gagtttaacc ggcgatattt ttgaagcagt cggcggagat aagctgaaag gtgccaaaaa
     1561 atggcagcct tacaagaaag aatgacagat ggatacgaca caaaaacgtg tggctttatt
     1621 catcattgga ttatctgttg tgatgtggct aagaaagcat aactgctgta cagtgatatt
     1681 tgtattgttg ttggcagcgt tagcatttag ctacacgatg gaacatttat attagctggg
     1741 gaactgaatt atggtaaaga atgtggttca tgaagagata ttacataacc gtgcagaatt
     1801 acagcaagcg gttgaaaaag aagaattttg tgcaaaatgt gataactcag tagtattcca
     1861 tttacgtgat aattatcacg aatttacgct tgggttagat actatattgc agtgtctgca
     1921 ttttgctgaa gagaagggag ctatccggat accttgaata ttgagtattc actcaatcat
     1981 tgccaaggag atggagtcgc attttatggt cgacttggta cagatgatct aatccagctt
     2041 ttcaatcatc tcaatccaca gaaacgtaaa cagaaaatgt ttgctaattt gctaacgcac
     2101 attgagtctt gggaaaccca cgatgatatt ggttttgaaa ttatacgaaa tcgctttggg
     2161 tatcgttact cgcattggaa tacgatggag ctttatgcta aaactgcaga tcagttagtg
     2221 tttttcaatg atagtgatgc tcgaagaaac tggtattttc cgacttcaaa agtgtataaa
     2281 tacaaaggct tgtgggatga gtttattcag gatttagaac agtatattaa gggtacttcc
     2341 cgtgaaatgg aatcggtcgg ttatcaaatt attgaagcct cgccgtatca aacggaaacc
     2401 gttttcaaat ttaatacgga aaactatcta gtagaactga aacgtaaacc ttctgagttt
     2461 tattcagagc ttgttgattg ggcatttggt gatatgtgtg attttgaagc aactatagag
     2521 cagatttgtc aagggcaact ctgttatgct gattttgaag caactgttac cgataagaaa
     2581 agtgaaattc agttaggtct tgctcgcttg agtggaataa gttactcgcc gaatgaccgt
     2641 tcgtttagtg gttatcgaca agagcttatt ggtgaggcga ttgcctctgc tcgccataat
     2701 gcagcacgtt ttgcctactt gcacgcctaa tttgcagatt tgtacttcaa tttgaccgct
     2761 tgtagtggtt aattttcacc ccgtggggaa atattttccc tattgggcaa gatgtttctc
     2821 cactttttaa tttggagaaa tgtgatgaaa aatgaacaca atttagagca acttaaatat
     2881 atttgggata accgcaacct tgaaaataaa gcctatcaat acctgtttca catttgcatt
     2941 gagccgatga aaaaaggaga ttggaatcgt tatacgattt ggcttaattt tcacggcaac
     3001 ttaatcaacg tgtcagacgt tgtatatcga gttgtggaat ctcaaaagct caaggatgac
     3061 atcaaaatgt ggtatgacaa taataccatt gtcgtccgat aaaagttgaa acaacaggct
     3121 tcatctcata tttactgcac cagttctatc aggggatagc ctatatttta ctggaacagg
     3181 gaatgattac tgaaactgaa gcagccgaga aaagttataa cttcagagct gcaaattgtt
     3241 ttcaacgttt gctttaatgg aggtgagata atggaaactc gcattgttaa agttatttac
     3301 gatgatggtg attatttctt tacccgaatc aatgctgatg aacaggctat ccgtaattac
     3361 tacgccatag gcagttcaat aggttttgat gatataagaa aaattatcgc tgttgaaatt
     3421 ttataaacca cccatagggg aaactatttc cccatagggt gatagcgttt ccccattttt
     3481 ctacccgaac tttggagaaa cacttatgaa cactcaacct aaatttgatt tataccaaca
     3541 catcactgac cgaattgttg tagcactgga acaaggtaca gccccgtggt taaaaccgtg
     3601 gagccatccg gaatgcaata tggcattacc tcgcaatgcg gtatctaacc gcttgtattc
     3661 cggtatcaat acactcttac tctggattgc tactgctgaa catggttaca aacagtgcaa
     3721 gtggattaca gcaaaaaaag ccaatgaact cggcggatat atccgtaaag gggaaaaagc
     3781 tactgtggtg atgaactacc gtcctgatga gagagagaaa ctcgatgagg aagggaatat
     3841 tgtgtatgac gaggaaggaa atccggaaat ggaacgacac tttttcatta ataagcacaa
     3901 tctgttcaat ctggaccagt gtgaaaattt accggtggaa ctgtacgatc ctgtatcaga
     3961 accgaaacaa cctaaatctg agccaaacca atacgatatt tttgctgaga ttcggcagat
     4021 tatcaaagga atggatttgc aggtggaaat gaagcctagc aatcgggcat tttatcaacc
     4081 aggtgaagat aaggtcgtaa tgccggagat gaagcagttt gcaaatgctc aagcgtttca
     4141 tagtgtattg ttacacgaaa tgactcacgc aacgggggca aataatcggc ttaatcggga
     4201 aggtattact tccggaaagg ctaaatttgg aaacaaagtc tatgcttttg aggagttgat
     4261 tgctgaaatg ggcggtgcat ttttatgtgc ctaccttggt tttgatactg tgcctcaaaa
     4321 tgcggcgtat attgaaagtt ggattcgagt attgaaagaa gataaacggg cgattgtccg
     4381 tgcttccgga aaagctcgtg aagcctgcca atacatgtta gacgcattaa acgtcgcaca
     4441 gttggctgac aaatacgatg tatttgaaga agctgcttaa caaatttgcc gaaaggcata
     4501 tctcaagggg agacctatcc ctttgggcat agtgtttccc tattttttat taaatggaga
     4561 aaagctatga caaaatcaac tgaaatacct cgtggacatg ataactgggt tattacaggc
     4621 agagacaatg atgggaaatg gcataaagaa cgttttcttt attattctcg ccgtgaagcc
     4681 atccaacagt ttcgtcgaaa acacaactgc gtaggtaaac atagtgttat tgaatcaata
     4741 acacctcaat ttggctggta ttaaccggct ttcccgtggg gagatactct cccttcggag
     4801 cattgtgtct ccctttttat tcacttaact agaggacaca atgttcaatc taaacttatt
     4861 taaacgccct attgagcttt caacgcttga atcttggtct aaattctgtg atgatgtcgg
     4921 taaagttgca tttattgcga tacctccgat gatttatagt aattatgatc tatgtatcaa
     4981 aataggaaac ataagtgctt tattggcaat tacctatgga ttcttattat caggcaggac
     5041 tatccgtcaa tttattgaga aggagaaatc aagatgggat taacaatcgg cttatggatc
     5101 attgctttta taggctttgc actggttttt tgggcaaata gcttactcaa agagcaagat
     5161 gaacgcctta aacagctaaa aaaatagctc aacttt
//
LOCUS       DANLXQ010000166         5126 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.166, whole genome shotgun sequence.
ACCESSION   DANLXQ010000166 DANLXQ010000000
VERSION     DANLXQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.166"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(14..673)
                     /locus_tag="PXD94_002284"
     CDS             complement(14..673)
                     /locus_tag="PXD94_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDL6287101.1"
                     /translation="MTTLGSRIKAYREQLGISQYEVAERCTSIDNRDKNNKWGQSRIA
                     NYERNNRTPDLDDIEILSKVLGVSPETLAFDTNVQLARPTKSNSYPLISNIQAGLWTE
                     AFDFKDSEGYDYIDTEIDAGPDAFFLRISGMSMEPKFSEGDLVLIDIRKRPHPGDYVA
                     AVNGTGEATLKRYRELGEWSESGNPHFELIPLNPDFPTLSSMKQDIRIIGVAVEHRSY
                     L"
     gene            803..1009
                     /locus_tag="PXD94_002285"
     CDS             803..1009
                     /locus_tag="PXD94_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6287102.1"
                     /translation="MNKIAEIRNQIGISQAQLAVQIGWGQPRIANYETGNRTPSIYVA
                     QKIVEGLNELGANVRIDDVFPVGN"
     gene            1030..1290
                     /locus_tag="PXD94_002286"
     CDS             1030..1290
                     /locus_tag="PXD94_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287103.1"
                     /translation="MPRNELTKNARAIADLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEATT"
     gene            1454..1624
                     /locus_tag="PXD94_002287"
     CDS             1454..1624
                     /locus_tag="PXD94_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287104.1"
                     /translation="MKNQRNDTQKSSLWQEFEYRKRLKNLIESGLSVAEIETRSQQIY
                     EELKSECTTEAN"
     gene            1599..2468
                     /locus_tag="PXD94_002288"
     CDS             1599..2468
                     /locus_tag="PXD94_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL6287105.1"
                     /translation="MNAQPKQINPILKLHPKQSEAKKVSVDDGYTQIPNELLKAILRS
                     GVLGWKGSYLLATVLKTLSWHKESDWFTHSQVCEMMNIEPTKYHINQLSAARKELIRE
                     NILFEDGKETGVNLSVFEWKMVYPEKVGSSRNNRELIPEKVGNGYPEKVGNTKETITK
                     EKINNEFTNVNSVQQPETSLPKKTKSEPVDYQGVMEEFNRAVEDTPIPQIRAMSDERK
                     RSVHALAKILRKEFGGYTRQHFADYFNDFVRQASSRKDRFYFGGLDGSAWVANFGYII
                     RSKTFYKTWEDSL"
     gene            2465..3826
                     /locus_tag="PXD94_002289"
     CDS             2465..3826
                     /locus_tag="PXD94_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDL6287106.1"
                     /translation="MSQNLKNITYEVEYLLVGALLKSGLNSKARDVLSWLEPEMFATF
                     QLGVIYEAIRKQALKDNVIDMLLLNTDYGQDFATLAEIMKNTISGANLDGYAEKVRQY
                     HQRREAQRVFLDVAGELQNARDEQLDAITSTGLAHLSKLLQRGGKVKPIDMNDLLEGY
                     LELFQERAKPSFKERLLFTGIEALDGKLGGINDTDICIVAGRAGNGKTETAITFTKNI
                     IENKGSVLFFSLEMSKEQIMDRLIASASGVNSVKLRNPEMMNDEDFARMGTAIQPLQN
                     QQLYIVDKSGLTAEEIVAIAESHIQEYGKVSAVVIDYIGLVRHGKLDGKINRTYQIGE
                     SMERFKTFCKNNHTPMILLAQLNRNADGSRPTNADLRDSGSLEQDASQIIMVHNQRNK
                     DGEPAPYTEWIVTKNRFGANGTVYMQFQQGRFVECDQAEAREYFQPKETKTSSNKRYG
                     AMQ"
     gene            3823..4359
                     /locus_tag="PXD94_002290"
     CDS             3823..4359
                     /locus_tag="PXD94_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL6287107.1"
                     /translation="MTDFDKNIKYKIIYADPPWKYKDSGCNGSAENHYTTMNIKEICD
                     LPVQDISDKDSILFLWVTYPMLPEGLKLIEAWGFNYKTIGFQWVKTNKKNKNSFFFGL
                     GRWTRGNTECCLIATKGKPSRVSNKVSQLIIEPIQHHSKKPDIVREKIVELMGDLPRI
                     ELFARNKTQGWDVWGDEV"
     gene            4450..4665
                     /locus_tag="PXD94_002291"
     CDS             4450..4665
                     /locus_tag="PXD94_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287108.1"
                     /translation="MITYKCPKCNGELEDLSINDEWGWFLDEPYRCNGHYTGRFPNIS
                     RDSTLNRTKSCGYFSKEEVKKVNGTQR"
     gene            4649..>5126
                     /locus_tag="PXD94_002292"
     CDS             4649..>5126
                     /locus_tag="PXD94_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL6287109.1"
                     /translation="MAHSVNDIVKAHGRRYKARLKIQMIKMQGGVLVPLNEREAEALV
                     SLKNGEQYEIEVIRTRNPAFHRKVFAFLNFCFEYWSADKTEWEFFDERKQFDTFRKNL
                     TVLAGFKEITYTLDGRMRVEAQSLSYGNMEQDEFEQVYKALINAAIKHVFNNTKDEN"
BASE COUNT         1698 a          904 c         1158 g         1366 t
ORIGIN      
        1 gctttggtgg actttacaaa tagcttctat gctcaacagc gacaccaata atacgaatat
       61 cttgtttcat tgagctgagt gttggaaagt cggggttaag cggtattagc tcaaaatgag
      121 gattgccgga ttcagaccat tcgcctaact cccgataacg ttttaaagta gcctcacctg
      181 tgccattaac cgctgctaca taatcgcccg ggtgaggacg tttgcgaata tcgattaaca
      241 ccagatcgcc ttcactaaat ttaggctcca tcgacattcc ggaaattctt aaaaagaaag
      301 cgtccggtcc ggcatcaatt tccgtatcga tatagtcgta gccctccgaa tctttgaaat
      361 caaaggcttc tgtccataaa cccgcttgta tattgctgat caacggatat gagttcgatt
      421 ttgtcggtct ggcaagctga acgtttgtat caaaagcaag tgtttctgga gaaacaccaa
      481 gcactttgct gagtatttca atatcatcaa gatcgggagt acgattgttt ctctcataat
      541 tagcaatgcg agattgcccc catttgttat ttttatctcg attatctatt gatgtacatc
      601 tttcagcaac ttcatattgg ctgattccta gctgttctct gtaagcctta attctactac
      661 ctaaggttgt catagcactc tcctattaga ttttcctcat tttaacacgc tccgttatat
      721 ccttgtgaat cacaaaatga aatttacaat tagttcattt tgtgatttaa ttaaaagata
      781 taaatcacaa agagaaattt gtatgaataa gatagctgaa atacgaaacc aaattggtat
      841 aagtcaagct cagttagctg ttcagattgg ttgggggcaa ccaagaatag ctaactatga
      901 aactggaaat cgtaccccat ctatttacgt tgctcaaaaa attgttgagg gattgaacga
      961 attaggtgca aacgtacgaa ttgatgacgt tttccccgtt ggcaactaat ttaccccaag
     1021 aggcttgcaa tgccacgcaa tgaattaaca aaaaatgcaa gagcgattgc ggatttgatt
     1081 catcgaaaat ccgcaacggt gacgcataag aaactagcaa gagcaatcgg actgagtgaa
     1141 tcacaattta gccgtacatt tgcagataat gtggaaatgg tggcggtgat tattgattac
     1201 ttaagtattg agcttgcgga taaagacgag ttactagagc tgaaagagaa acgatataag
     1261 cgaataaatc tcgaagaagc gacaacatag aagttaccaa caatgaaaaa ttttttagaa
     1321 aaattcaaat cattctacca cagtaaaaat tcaattttta ttagagtggc aatttttatg
     1381 ctgcttgcgt ggtgggtttt taacaattaa aaaaccaccg ctggaacggt ggtaatttac
     1441 aaggagttac cttttgaaaa atcaacgcaa tgatacacaa aaaagctcct tatggcaaga
     1501 gtttgaatat cgaaaacggt taaaaaatct cattgaaagc ggtttatccg tagccgaaat
     1561 tgaaacccgt tcacaacaaa tttatgagga gctgaaaagt gaatgcacaa ccgaagcaaa
     1621 ttaacccaat tttaaaattg caccctaaac aatcagaggc taagaaagtg agtgttgatg
     1681 atggttatac acaaattccg aatgagttat taaaagcaat tttgcgatcg ggtgttttag
     1741 gttggaaagg ctcttactta ctggctaccg tcttaaaaac attgtcttgg cataaagaaa
     1801 gtgattggtt cacgcattcc caagtgtgtg aaatgatgaa tattgagccg acaaaatacc
     1861 atattaatca actttccgct gctcgaaaag aacttatccg tgaaaatatc ttgtttgaag
     1921 atggtaagga aacaggggta aatctcagtg tttttgaatg gaaaatggta tatcccgaaa
     1981 aagtagggag ttcccgaaat aatagggaat taattcccga aaaagtaggg aatgggtatc
     2041 ccgaaaaagt agggaacaca aaagaaacta ttacaaaaga aaaaataaat aatgaattta
     2101 ctaacgtaaa ttctgtgcaa cagcccgaga cctctttgcc gaaaaaaaca aaatctgagc
     2161 cggtcgatta tcaaggagtg atggaagaat ttaatcgtgc tgtggaagac acaccgattc
     2221 cgcaaattcg ggcgatgagt gatgagcgaa agcgatcggt tcacgcatta gcaaaaatct
     2281 tgcgaaaaga atttggtggc tacaccagac aacactttgc cgattacttc aatgattttg
     2341 tccgacaggc aagtagccgg aaagacagat tttattttgg cgggcttgat ggctcggctt
     2401 gggtagcaaa tttcggctac atcattcgtt cgaaaacgtt ttataagact tgggaagatt
     2461 cgctatgagc caaaatttga aaaatatcac ttacgaggtt gaatatttgc tggttggagc
     2521 attgctgaaa tccggtctta actcgaaagc tagggacgta ttgagctggt tagagcctga
     2581 aatgttcgct acctttcaac ttggggtgat ttatgaggct atccgcaagc aggcgttgaa
     2641 agataacgtg atcgatatgc tgttactcaa taccgactac ggacaagatt ttgccacgtt
     2701 ggcagagata atgaaaaaca cgatcagcgg tgcaaactta gacggctatg cagaaaaagt
     2761 ccggcaatat catcaacgcc gagaagccca gcgagtgttt ttagacgttg caggtgagtt
     2821 gcaaaatgcc cgagacgagc agttagatgc tatcacttca accgggttag ctcacctaag
     2881 caaattatta caacgtggcg gaaaagtgaa accgattgat atgaatgatt tgctcgaagg
     2941 ttatttggaa ttgttccaag aacgtgcaaa accaagtttt aaagagcgtt tgttgtttac
     3001 cggtatagaa gcattagatg ggaaactagg cggtattaac gataccgata tttgcattgt
     3061 agcaggacgt gccggtaacg gtaaaaccga aacagccatc acgtttacta aaaacattat
     3121 cgagaataaa ggttcggtgc tgttcttctc gctggagatg agcaaagagc agattatgga
     3181 taggctgatt gccagtgcaa gcggtgtaaa ttcagtaaaa cttcgcaatc cggaaatgat
     3241 gaatgatgaa gattttgccc gaatgggaac ggcgattcag cctttacaaa atcagcagtt
     3301 gtacattgtt gataaaagcg gattaaccgc agaagagatt gtggcgattg cagagagcca
     3361 tattcaagag tacggcaaag tgagtgcggt agtgattgat tatatcggtt tggttcgaca
     3421 cggcaaacta gacggcaaaa ttaaccgaac ctatcaaatc ggtgaaagta tggagcggtt
     3481 taaaactttc tgcaaaaaca atcacacacc gatgatttta ttggcacagc ttaaccgcaa
     3541 tgcagatggc agtcgcccaa ccaacgcaga tttgcgagac agtggcagtc ttgaacaaga
     3601 tgcaagccaa atcattatgg tgcataacca acgcaacaaa gacggcgagc cagcccccta
     3661 tacagaatgg attgttacta aaaatcgctt tggagcaaat ggcacggttt atatgcaatt
     3721 ccagcagggt aggttcgtag aatgcgacca ggcagaagcg cgggagtatt tccagccgaa
     3781 agaaactaaa acatcatcaa acaaacgcta tggagcaatg caatgacaga ttttgataaa
     3841 aatataaaat acaaaattat ttacgcagat ccgccttgga agtataagga tagtggctgt
     3901 aatgggagtg cagaaaacca ttacacaaca atgaatatca aagaaatatg tgatttaccc
     3961 gtccaagata tttcagacaa agattctatt ctgtttttgt gggttactta ccctatgtta
     4021 cctgagggct taaagttgat tgaggcgtgg gggttcaact ataaaacaat agggtttcaa
     4081 tgggtgaaga caaataagaa gaataagaat tcttttttct ttggcttggg acgatggact
     4141 agaggtaata ccgaatgctg tttaattgca acaaaaggaa agccatcgag ggtaagcaat
     4201 aaagtgagcc aattaattat tgagccaata caacatcata gtaaaaagcc tgacattgtc
     4261 agagaaaaga tagttgagtt gatgggtgac ttgccaagaa tagagttgtt cgccagaaat
     4321 aaaacccaag gttgggatgt ttggggggat gaggtgtgat tgtttcaagc tattgttacc
     4381 caaagtgcgg tagggtttta ttaaaaaggt tggcggttgt gaaggataac gaaggttggg
     4441 acggtttaga tgattactta taaatgcccc aaatgcaacg gagagcttga agatttaagt
     4501 attaatgatg aatggggttg gtttttagat gagccgtatc gttgcaacgg acactataca
     4561 gggcggtttc caaacattag ccgagatagc acgttaaatc gcacaaaatc ctgtgggtac
     4621 tttagcaagg aagaggttaa gaaagtaaat ggcacacagc gttaatgaca tcgtaaaagc
     4681 ccacggcagg cgttataaag caagattgaa aatacagatg ataaaaatgc aaggaggcgt
     4741 tttagtcccc cttaatgagc gtgaagctga agcattggta tcacttaaaa atggcgagca
     4801 atatgaaatt gaggttattc gcactcgcaa tccagccttt catcggaagg tcttcgcctt
     4861 tttaaatttt tgctttgaat attggtcggc agacaaaacc gaatgggagt tttttgatga
     4921 gaggaaacag tttgatactt tccgaaaaaa tctgactgtt ctggcaggat ttaaagaaat
     4981 cacatacacc ctagatgggc gtatgcgtgt agaagctcaa agtcttagtt acggaaatat
     5041 ggaacaagac gagttcgagc aggtttacaa agcattgatt aatgcagcta tcaagcacgt
     5101 tttcaacaat accaaagatg agaata
//
LOCUS       DANLXQ010000167         5059 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.167, whole genome shotgun sequence.
ACCESSION   DANLXQ010000167 DANLXQ010000000
VERSION     DANLXQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.167"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..227
                     /locus_tag="PXD94_002293"
     CDS             <1..227
                     /locus_tag="PXD94_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL6287110.1"
                     /translation="ELALKGNCTKKVTVNYTLDTRKYYGSVFNELLEEPFLLSEYDAY
                     QFTNEVIKFHSEESLKAWGGCLIWEQNNGS"
     gene            359..1057
                     /gene="aphA"
                     /locus_tag="PXD94_002294"
     CDS             359..1057
                     /gene="aphA"
                     /locus_tag="PXD94_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL6287111.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1238..1999
                     /gene="udp"
                     /locus_tag="PXD94_002295"
     CDS             1238..1999
                     /gene="udp"
                     /locus_tag="PXD94_002295"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL6287112.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
     gene            2074..2659
                     /locus_tag="PXD94_002296"
                     /pseudo
     CDS             2074..2659
                     /locus_tag="PXD94_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2670..3155
                     /locus_tag="PXD94_002297"
     CDS             2670..3155
                     /locus_tag="PXD94_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL6287113.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(3193..4062)
                     /locus_tag="PXD94_002298"
     CDS             complement(3193..4062)
                     /locus_tag="PXD94_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL6287114.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            4192..4983
                     /locus_tag="PXD94_002299"
     CDS             4192..4983
                     /locus_tag="PXD94_002299"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL6287115.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT         1576 a          909 c         1060 g         1514 t
ORIGIN      
        1 aagaattggc actaaaagga aattgtacaa agaaagttac ggttaattat actcttgata
       61 cacgtaaata ttatggatca gtatttaatg aattattaga ggagccattt ttattaagcg
      121 aatatgatgc ctatcaattt acgaatgagg tgattaaatt tcattcagaa gaaagcctta
      181 aagcttgggg aggttgcttg atttgggaac agaacaatgg ctcataagcg ttaagcgtaa
      241 tcgtttgctt gtgatcatat tcacaaaata aggtttttgt attagctaag agtgcctaat
      301 aagattaaaa taggtatggt taatcatatg gaggtatttt ataaaattta cattaaaaat
      361 gacggcagcg acaatgtttg catctcttat tgctttttca gcacaagcag gcggaccaaa
      421 agtagcttat acccagccgg gaattgatat gcgaacacat gaagcacagc aagcgccaat
      481 tcattgggta tctattgagc aaattaaaga aagcatcaaa gatttacccc ctatggcggt
      541 aagttttgat attgatgata ctgttgctgc aactagcggt tgcttccact acggaaagca
      601 aaaatattcg ccagacgatt tcagctactt aaaaaatcaa gatttttggg acgaaattaa
      661 tgctggctgt gataaatatt ctttgccaaa gcaagttgct attaatttaa taaaaatgca
      721 ccaagaacgt ggtgatcaag tttatttaat tacggggcgt accgcaggta aaaatgaaca
      781 agttacaacg attatgcaaa agtatcttgg tattaaaaat atgcaaccgg taaattttat
      841 gggggggaaa gcacattcaa caaaatatga taaaacccct gcgattattg aacataaggt
      901 gcaaattcac tatggtgaca gtgatgatga tatcttagct gcccgtgaag ccggtattcg
      961 aggtattcgt atacttcgtg cagcaaattc taattatacg ccattccctc aggccggagg
     1021 atatggtgaa gaagttgttg tcaattcaag ttattaattt cttagtctga aatatggcca
     1081 ccattgcgtg gccatcattt tttatttata taatgacgaa accgtttacc tttgatctag
     1141 ttcacaaaat caaactttgc ttgcaaaaaa ttgtcgtaaa ttaaccgctt gctaagatag
     1201 aatggctcat atccagttag ataaaaagga gataaaaatg tctgaagtat ttcatttagg
     1261 tttaaccaaa gccatgctca aaggtgcaaa aattgcaatc gttccgggcg atccggctag
     1321 aagtgaacgt attgccaaaa aaatggacaa cccagagttt ttagcctcaa cccgtgaatt
     1381 tacctcttgg ttaggttatt tagatggcga accggtggtg gtttgttcaa ccggtattgg
     1441 cggaccgtcg gtgtcgattt gtgtggaaga gttagcacag ttaggtgttc gcacattctt
     1501 acgcattggc acaacaggtg caattcaaga gcatattaat gtaggcgata ttttaattac
     1561 caccggagcg gtgcgtttag atggtgcgag ccaacatttt gcaccaatgg aatatcctgc
     1621 agtagcgaat tttgaatgta ctaatgctct ttatgcagca gcagtggaat caggcgttac
     1681 accttatgtg gggattacag cttcatcaga cactttctac ccggggcaag aacgttacga
     1741 cactttcacc ggcaagatct atcgtcattt ccaaggttca ttaaaacagt ggcaagatct
     1801 caatgttatg aattttgaaa tggaatccgc cactctcttt acgatgtgtt cggcattagg
     1861 cttgcgagca ggtatggttg ctggtgtaat tgtaaaccgc actcaacaag agattccgaa
     1921 tgaagcaacc attcacgaca ccgaacataa agccgtttcg gtagtagtgg aagcggcaag
     1981 aaaattaatt aaaggctaat ctttaaattt aaaagcaaaa aaggtaaatt gtctttgtta
     2041 caatttacct tttttctttt tagggagctt tctatgaaaa aatggttgat gttaattaca
     2101 ttaattttcg gcattaccgc ttgtttttcc agtttagaat ttgatgaaag taaacaagaa
     2161 aaaatcaatt cattcttgat tgttggtaat aaattatatg tggtgggtga actgcacgat
     2221 tatgaatttg agcatcagca agttgcggaa ctgaataatt ttctcaaaag cctaattgct
     2281 aataaaatca aatctgttga ggcaaacttc gaggttaagg aatccaaaaa agtggaaggt
     2341 agttatagaa ttattttaga tgggaaatca cttacagaaa gtgaaagaag tacggtgatg
     2401 gtttaattca gctcaaaatc catctattac ttatcaggcg gaagggaact tggtgaaatt
     2461 agccaataga gaagagcttt tgcaaaaatt ccgatttaaa caagcgcttg aggcgaatgt
     2521 gcgttattac aaaatgaaaa gtggtcttca aaatacggct gaaggagcgt taagtattgt
     2581 aatgttgcct gttgctgtag tagctgtttt accattctat atgttttggg ttggtgcttg
     2641 tgccacttac ggatgttaaa taacttttta tgtattctct atacctgaaa attttatcgg
     2701 aatgcgattg gtgttgtgat ccaaaaacac tttctaccta ccagcaagaa tggcttctgc
     2761 acgaagggtc acttacccaa aaattgtatg atgtcgcaac caatttcaac gtggaagtag
     2821 tacaagaaaa gtggattgca aaaaacagtg aaaaaacgac cgcttgtaat gaagatgttt
     2881 ggtcgcgaga agtgttactg aaagatggtg aaaaaaattg gatttttgcc caaactcttg
     2941 tgccaagatc aacaatcgaa aatatcgctc aaaacgtgtt ggaattgggc aatcagccga
     3001 ttggtttatg gctattttct caaaatccta ctcgcaccaa attagaatgg acacaaacag
     3061 agaacggttt attcgctcgg cgtggggtgt atgaaattag agggtattct ctggaaattc
     3121 gagagctttt tttagaaacc tttccttatt tataaaaaaa tcggttggta atgacaccaa
     3181 ccgattttca tcttattttg ccaagaactg ataaattgca gccatatcca cttcgctcaa
     3241 gcctgaatca ttggcttgat tgtaggtttt agtggcttgc tcaataagcg ggtaagattt
     3301 gcctaatttc gcaatttctt cgcttgccaa attaaaatct ttggtcatat gtttcatcat
     3361 aaacgcaggc ggaaattcat tagcttggta catcggcact tttgcttgga agatcgggct
     3421 gttcataaac gagcctgaaa tcatttcgat tactttctct ttcggaatgc catattggtc
     3481 ggcgaagttt aacgcctcgc cataggcttg aatgaagatg cccagtagcg agttgatcat
     3541 caatttaatc ccaccggctt tacccacttc accataatag aaagtggtgg tactaaaggc
     3601 agcgaaaagc ggtttgagca actcaacggt ttcttcttta ccggcagcca gtaccaatag
     3661 tttacctgct tctgccactt tgcttgaacc ggaaaccgga gcttcaacaa attctgcacc
     3721 gtgttgggct agtagagctt ctaccgcctg gttttggctt ggggaaatgg tgctcatatt
     3781 caccactact ttgccgttta attcagccaa cacttcatcg cttaacacct gttgcacggc
     3841 ggcatagtct gcaatcatca agaaaatcac atcgtaggct ttcaccaatt ccaacacaga
     3901 aggatataat gtgccgcctg cttcaactac cttttctgct tttgctgggt cgcggttgta
     3961 aacgcccacg ttcactccgc tatttagcaa atgttttgcc atcggttcgc ccatttgccc
     4021 taaaccaatc caaccgactg cttgtgtatt aatgtttttc attatttttc tccttactga
     4081 ataaagcaaa ttgattattg cgattttccc tcatcaagtc aaatagagag aacttgaata
     4141 tagcaatgga aaagagtagc attttagcac ttactttttt gaacaatacc aatgaaacca
     4201 acaattcttt tatacgattc cggaatgggc ggattgacga tttacgatga aatccgaaaa
     4261 gtgttaccgc acgctcacta tttatattgc tttgataacg cttttttccc atattccgag
     4321 aagtcggaag cagttttaat tgagcgagcg gtcaaaattg tgcaaaaaat tgcagaaaaa
     4381 caaccgcttg atatggttgt tgttgcttgt aatacggcaa gcacagtggt tctgccggca
     4441 ttgcgtgagc attttaattt tccgatagta ggaactgtac cggcaattaa accggcagcg
     4501 gcaatttccg aaactaaagt catcggcttg ctcgccacta aaggcacggt tgctcgccct
     4561 tatgttgatg aattgataag aaaatatgct caggattgta cggtagaaaa aattggcaca
     4621 accgatttgg ttgaaattgt tgaagaaaaa caacgaagcg gtaaagtgga tatgaaccga
     4681 ctacaaaaag tggtcgaggc ttggcaaggt aagccggaat tggatacggt tatactcggt
     4741 tgtacccatt ttcccttagt caaagctgaa ttacagcaat tattgcccga tgtgaaatat
     4801 tttgttgatc cgggtgtagg tattgccaaa cgggtgctaa gccttttgga agaacgcaaa
     4861 tttcaacaaa aagccgattt tgaaaatcaa gcattctgca ctaaacaaag ttctgcattt
     4921 aatatcaaac aagacgttat gcagaattgg ggatttaata atctttcggt gattgatatt
     4981 taaacaaaac aatctgtttt gatgaaaaaa ggttcagttg agcaaaaact ttaaaaaagg
     5041 gcttgcactc aattttaaa
//
LOCUS       DANLXQ010000168         4970 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.169, whole genome shotgun sequence.
ACCESSION   DANLXQ010000168 DANLXQ010000000
VERSION     DANLXQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.169"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1338
                     /locus_tag="PXD94_002300"
     CDS             <1..1338
                     /locus_tag="PXD94_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL6287116.1"
                     /translation="KEMNATSVSSIPKKESVTNVPTLDKKETEQNLTQLQKDFSAHQL
                     ENQKAKQSMINAQSELRRLNSQLNVLQKYVNSRRLGYYTQQAVLEQISIIQNKIKQTQ
                     TIFNDANATVKLTDQKLEEAKLALGSVNDLVLIKASAPAMQATNQDTSMMNIIQADWI
                     SQYANTALSELSAQANSALQISNSLDRQLFKQSDKFNVWSSVEHQKTEHKSDLYRPYK
                     QQTNLTQLGIQMPIDNGLMFGVALSKNHANAEFNEGVNGKSNLLMASLYGKWQSQQGT
                     FISLDGSYGKAKNQLYLFGENHFTRRISSIGANIGHQFDLAGVQIQPTIGARYYHFSG
                     QDYTLGGAKISSPNTHFMTYQAGLKASKTFSLDDWKVEPSITTHYVDASNKRLAVNVN
                     NNNINQRFGRYLKTEAGIRFEHNQWQFDLHAGFINGNEIKKQHFAGVKLGYSW"
     gene            complement(1393..2487)
                     /gene="mobA"
                     /locus_tag="PXD94_002301"
     CDS             complement(1393..2487)
                     /gene="mobA"
                     /locus_tag="PXD94_002301"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL6287117.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            2574..2843
                     /locus_tag="PXD94_002302"
     CDS             2574..2843
                     /locus_tag="PXD94_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL6287118.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     LSDEVLIYQLKMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            2907..3545
                     /locus_tag="PXD94_002303"
     CDS             2907..3545
                     /locus_tag="PXD94_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL6287119.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            3629..4405
                     /locus_tag="PXD94_002304"
     CDS             3629..4405
                     /locus_tag="PXD94_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL6287120.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNSVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKV"
     gene            4415..4669
                     /locus_tag="PXD94_002305"
     CDS             4415..4669
                     /locus_tag="PXD94_002305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase"
                     /protein_id="HDL6287121.1"
                     /translation="MKAEIVPHFGPVSFQQNALYNQEKPVVRQMTACSLIGSRESIEQ
                     QLKSLKARIEIDEIMAVSYIFDEQKQHQSYTWLKEIADKV"
     gene            4671..4964
                     /locus_tag="PXD94_002306"
     CDS             4671..4964
                     /locus_tag="PXD94_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL6287122.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT         1612 a         1006 c          958 g         1394 t
ORIGIN      
        1 aaagagatga atgctacatc tgtatcctct attccaaaaa aggaatctgt tactaatgtt
       61 cctactttag ataagaaaga aactgaacaa aatcttactc aactacaaaa agatttttca
      121 gcacaccaat tagaaaatca aaaagcaaaa caatctatga taaatgctca atctgagcta
      181 agacgactca attcacaact gaatgtattg caaaaatatg tgaattctcg tcgcttaggt
      241 tactatactc agcaggcagt tttagaacaa attagcatta ttcaaaataa aattaaacaa
      301 acacaaacaa tatttaatga cgctaatgca actgtaaaac tcacagatca aaagctagaa
      361 gaagccaaat tagctctagg ctctgtaaac gatcttgtat taataaaagc ctctgctcca
      421 gcaatgcaag caactaatca agatacgagt atgatgaata ttattcaagc agattggata
      481 agccaatacg ctaacacagc actttctgaa ctctcggcac aggctaattc tgctctgcaa
      541 atcagtaata gcttagatcg ccaactcttc aaacaaagcg ataaattcaa cgtatggagc
      601 agcgtcgaac atcagaaaac cgagcataaa tcagatttat accgcccgta taaacaacaa
      661 accaacctga cccaactggg catacaaatg ccgatagata acggtttaat gtttggagtt
      721 gcattatcta aaaaccacgc taacgcggaa tttaacgagg gtgtaaacgg taaatcgaat
      781 ctactaatgg caagcctata tggtaagtgg caatctcaac aaggcacttt tatcagcctt
      841 gatggcagct acggtaaagc aaaaaaccaa ctctacctat ttggtgaaaa ccactttacc
      901 cgccgaattt cctctattgg tgctaacatt ggacatcaat ttgacctcgc aggagttcaa
      961 attcagccaa caataggagc aagatactac catttcagcg gccaagacta tacactagga
     1021 ggagcgaaaa tcagctcacc aaatacccac tttatgacat atcaagcggg tctaaaagct
     1081 agtaaaacct tttcattgga tgactggaaa gttgaaccaa gcattacaac ccactatgtg
     1141 gatgcaagta acaaacgctt agcagtaaac gtcaataata acaatatcaa ccaacgtttc
     1201 ggtcgctatc tgaaaaccga agctggtatc cgatttgaac ataaccaatg gcaattcgac
     1261 ttacacgctg gtttcattaa cggcaacgaa atcaaaaaac aacattttgc tggtgttaag
     1321 ctaggatata gctggtaaga aaaagcccct agaactattt ctaggggctt tttctactct
     1381 taaaaagagt atctaacttg ccttattttc taaccaattc ttaataaaat ctgctattcc
     1441 cccaatatta ttaatatcca accaattttc tctctcaagc gggtaatccg ttgcggtggc
     1501 aatcgtccat tggtctagct ctggcaatgg tttttcaatc cctttgcggt ggagctgaat
     1561 ttttggcaag gtttcgtgtt taaagccttc tacgaaaacg agatcaacct cttgcgggtt
     1621 gaattttgca attaattgag aaaattcgac cgcttgtttg gtttctgtca ttaatgccca
     1681 acgttcatcg cacacaatca ttgtcgggtt tgctcctgct tcacgtaggc ggtagctgtc
     1741 tttgcctttt ttatctacat caacgttatg gtgggaatgt ttaatcagcc caacccgaat
     1801 accgcaagcg gtcaattttg ggattaattt ttccaataag gtggttttac ctgtgccgct
     1861 gtagcctgta attgcaaggt tttttacagg gaattggctt actcggctta aatcttccaa
     1921 tgtattcacg ttggtgaatg ccgatttttg ttcggaaaaa tcaactgcca cgcttttttg
     1981 tgattggaag aacgcaagca attgctgttg gtttgagcct aaataggctt tcaaatcttc
     2041 ttgtaccgaa cgatgaatca acgcaaaagt gggatgtgga cgctcaccat catgggcata
     2101 tgcaatttgt acgttattta tccgcaaagc agtttttaat ttttgcaata acagctcagg
     2161 cataaagggc gtatcgcaag gcacaaaaag caaatagtca ctttcaattt gctcaaatcc
     2221 tgccagcatt ccactgagtg gaccttgaaa atcaggcaag ctatcttgat agtaagaaaa
     2281 agcaggataa agctgctgat attgctcaat agaacgattg acattaagcc aaatgtcact
     2341 aacttgtggt gaaagtcggg ctaaaatgtg tgaaataagc ggttttcctt caaaaagttg
     2401 taaccctttt tctaccccat tcattcgacg agctaaaccg cccgctaaaa tcacggctga
     2461 aatgcgttct tctattgttt tactcacagc tttatccctt aaaattttgc aaaaataaga
     2521 gtaaaagtat attatttcgt ttaatttcca acaagttttt taaggggtaa aagatgaaat
     2581 gcgaacgttt agaagagtta ttagagttac ttggcgaaca ttggcgtaaa aatcctgact
     2641 tacacctcat tgatattttg cagcagcttt cagttgaagt gggcgagcct gataatttca
     2701 aagcgttaag cgatgaagtg ttaatctatc agcttaaaat gcgaaatgca ggcaaatttg
     2761 agcctattcc cggcataaaa aaagattatg aagatgattt taaaacggct ttattgcgag
     2821 ctcgtggaat tttaaacgat taagattgtc ttatccgtta gctagtttag catttattat
     2881 ttatcaacat ttttgaagga atcattatga aaaaattggc attaaaaacg gcatttgttg
     2941 cactttctgc cctttttaca tttaactcaa cggcaatcgc tgctgatcca accgcaggca
     3001 aagaatatat cgaagtgcgt aaagcacctt ctgcacaaaa agaagtggtg gaatttttct
     3061 cattctactg cccacactgt tatgattttg aattaagcta caaaatccca agccaaatca
     3121 aagaaaaatt accaagcgat agcaaattag tgcaatatca cgttaatttc ttaggtcgcc
     3181 aatctgaaga tttaactcgt gcttgggcat tagcaatggc attaggtgcg gaagataaag
     3241 taaaaaccgc attatttgaa ggtgctcaaa aagacgcatt caaatcaatg gacgatattc
     3301 gctctgtatt cttagcaaac ggcattacag ctgaacagtt tgatagcggt attaatagct
     3361 ttgcggtaaa tggcttagtg aataaacaag ttcaattagc ggaagacttc caaattcgtg
     3421 gtgtgcctgc attcttcgta aatggtcaat accaactcaa cttagaaggc tttgcagatt
     3481 cttcatcaac gaatgatttc atcaaacgtt atgttgatgc ggttgtgttc ttaagcaaga
     3541 aataacccct taaaatagtt agggttttta caatgctgag taggtaaatc ctattcagca
     3601 ttttttatat gcaaactcgg agaacaaaat gacaaagtta tctatcctaa accttgtacc
     3661 tattcgtgaa gggcaaactg ccaaacaagc ggtcgattct atggtgaaat tagcaaaaca
     3721 cgcggaaagt atcggcattg aacgctattg gattgcagag caccacaata tgaaaaacct
     3781 tgccagctcg gcaacacagc tcctcattca gcatgccctt tctcacactg aaaaattaag
     3841 agtgggatca ggtggcgtga tgttgcctaa ccatagtcct tatattgtgg cagagcaata
     3901 cggtacgctt gaaacactct accctaaccg tgtagaactt gggcttgggc gagcaccagg
     3961 caccgatatg cgaaccgcta ccgctttacg ccgtaatgca caaagtctgc catttcctga
     4021 tgaaatcagc gaactaaaag gttattttga aggcacaaat tctgtgtctg cctaccctgc
     4081 acaggggttg aatgtgccgt tttatatttt aggctcaagc cctgaaagtg cctatttagc
     4141 agcagaacgt ggcttgcctt atgcctttgc ttcacatttc gctcctcgtt ttatggacga
     4201 tgcggtgcga atttatcgca cacactttaa gccgtctgaa gtgttagata aaccctatgt
     4261 gattttaggt gtgaatgcga ttgcggcaga aaccgaccaa gaggcagaac agcttgctac
     4321 cactcagact cagtttttct taaatgttgt taccaatgcc caaaactatt tacaaccgcc
     4381 cgtcgaatcc gatgataaag tgtgaaaaaa ttttgtgaaa gcggaaatcg taccgcattt
     4441 cgggcctgtt tcgttccaac aaaatgcgtt atacaaccaa gagaaacccg ttgttcgcca
     4501 aatgactgct tgttcactta tcggtagccg agagagcatt gaacaacagc tcaagtcatt
     4561 aaaagcacgc attgaaattg atgaaattat ggcggtaagc tacattttcg atgagcaaaa
     4621 acagcatcaa tcctatactt ggttaaaaga gattgcagat aaggtgtgat atgagcaaaa
     4681 caatgcaaca aaaagccgct gatgcacata atatgtgtaa gctaaaaggc gactctatgt
     4741 tagataactc agaccgccaa attgcctttg aagcagtgta tgatagtgaa gaaatagctg
     4801 tacaagcggt cgaatttttc acacaaaaag caaaatcggt agaaaccgag ccttgcgaca
     4861 ttcaaagtga aatcacaccc attatagatg gctttctgat gaaaatgtgg ataactttca
     4921 gttgccaagc agaagtaatc ttatttcaaa tggcggtgag atagatataa
//
LOCUS       DANLXQ010000169         4939 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.170, whole genome shotgun sequence.
ACCESSION   DANLXQ010000169 DANLXQ010000000
VERSION     DANLXQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.170"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(50..601)
                     /locus_tag="PXD94_002307"
     CDS             complement(50..601)
                     /locus_tag="PXD94_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287123.1"
                     /translation="MKGIMKSILVFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAIAY
                     GATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYYSG
                     VIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQILH
                     LCVKTIAEDENESQSKGKTKKHK"
     gene            694..1545
                     /locus_tag="PXD94_002308"
     CDS             694..1545
                     /locus_tag="PXD94_002308"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL6287124.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            complement(1599..2042)
                     /gene="lysM"
                     /locus_tag="PXD94_002309"
     CDS             complement(1599..2042)
                     /gene="lysM"
                     /locus_tag="PXD94_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL6287125.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            2281..2718
                     /locus_tag="PXD94_002310"
     CDS             2281..2718
                     /locus_tag="PXD94_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL6287126.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLGKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            2712..3155
                     /locus_tag="PXD94_002311"
     CDS             2712..3155
                     /locus_tag="PXD94_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL6287127.1"
                     /translation="MLKNLSWRAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFTKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLARELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            3152..3894
                     /locus_tag="PXD94_002312"
                     /pseudo
     CDS             3152..3894
                     /locus_tag="PXD94_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            3896..4066
                     /locus_tag="PXD94_002313"
     CDS             3896..4066
                     /locus_tag="PXD94_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287128.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            4056..4649
                     /locus_tag="PXD94_002314"
     CDS             4056..4649
                     /locus_tag="PXD94_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287129.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
BASE COUNT         1581 a          971 c          944 g         1443 t
ORIGIN      
        1 aaaaagcggt aaaatttttt aaaaatttta ccgcttgtca tagcttaact tatttatgct
       61 ttttggtttt acctttagat tgcgattcat tttcatcttc agcaatcgtt tttacacaaa
      121 ggtgcaatat ttgctcaacc ccatttttga tatactcatc tccacgaact ttgttcttct
      181 gtaaatctaa catcgcatcg tgaatgccct gggtcatact cgctggttga acaagccccc
      241 aacaattagg atctaaatga ttgaaatttg cctgcaaatc tgcggcataa atcacaccac
      301 tataataagc atacacatta tggtctaaca ttcgattaat cgttattgct ctaatttgct
      361 caattggcaa tgtaaccttt ttagtgtacc aatcatttac cccattcata aaggaattga
      421 tatcatagct ttcatttact ctatccgcat aagtctgagc cgttgcacca taagcaatag
      481 cataagattt ctgatcaaca tcattatcat ttaaacgtgt ccaaccgctt ttattcgatg
      541 aacaagctgc taaaaaaaca gcggtggaaa aaaccagaat ggatttcatt atacctttca
      601 tatccataac tccttattgt gttgtgtcat aaagttttag ctattttacc gaataaatgg
      661 caaaattatc taaaattttt tatgaggtaa ataatgaaac agtatttaga cttatgtaac
      721 cgcatcgtta gcgaaggcaa atgggttgca aatgaacgta caggcaaaca ctgcctcaca
      781 gtcattaatg ctgacttaac ttatgacgta gaaaaaggag aatttccttt agttaccact
      841 cgccgtagtt attggaaagc tgcgattgcc gaacttttag gctatattcg aggctatagt
      901 aatgctgctg atttccgtca attaggcaca aaatcgtggg acgctaatgc aaatgaaaat
      961 gcacaatggc ttgctaatcc ctatcgaaaa ggggaagatg atatgggctt ggtttacggc
     1021 gcagtagggc gtaatttccc gaaaccagac ggtggctcag tcgatttatt gcgacaaatt
     1081 gtggacgatt tgaaaaaagg aattgataac cgtggtgaaa tttatacctt ctaccacccc
     1141 ggcgctttcc atatgggctg cttacgtcct tgtttacaca gccatcattt ttctctactt
     1201 gatggcacgc tttacctgaa tagtactcag cgttcggcag atgtaccgct tgggttaaat
     1261 tggaatatga ttcaatgcta taccttttta gctttaatgg ctcaaattac agggcataag
     1321 gcaggacaag cgttccataa aattgtcaat gcgcatattt atgaagatca actagaatta
     1381 atgcgtgatg ttcaacttaa gcgagaacca ctcaatgcac ctaaattaat cattaatcct
     1441 gatataaaat ccttagaaga tttagaaact tgggtgagct tagatgattt caaagttgaa
     1501 ggttatgaat accaccctgc cattcaatat ccattttcag tctaacacca cctacaaaaa
     1561 gagctaaact ttcgaccagc ttagctcttt caatttgatt ataaaccttt cgggatacga
     1621 attttttgcc ctgggaagat tttattttcg tctttaataa cttctttatt tgcttctaca
     1681 atcgctttat attttgcacc gttaccataa gcttttgtgg cgatttccca caacgtatcc
     1741 cctttttgga taacataaag ctcatcatca cttgcaagtt gcacgtcatc aggcaaggtc
     1801 acttcatcaa tatttacctc agtgatacca gccacattac ccgccatcaa taccgctttc
     1861 tcaacagctg ctgcggttga agcaatgcct gaaattttcg ctaccccgtt ttcaaccgtt
     1921 acgtttacat tttcaacgcc cggattgtct tccgccaaat gctctgtaac cgcttttgaa
     1981 gcatcttctt ctttattaaa taatttacga ccaatgtcgc caacaaaatc aaataagccc
     2041 atagtaagtt ccttataaaa tagctaaatg aagtgggtat tctacacaat ttccaactat
     2101 tgaaatagtg cattgttctc ttagaacgtc taaaagctaa ttagttcctc aaggcaacgc
     2161 aacacaagtg gtcatttttt ctcaattttt tgcaaaaaca aacgcttaga ttttatattt
     2221 tactcatttt cagctagaat attaagttta aatatctgat tgaatttctg ggaacattct
     2281 gtgaaacaag tacaatttta tctattaagc caagccgatg cagccgagct ttccgctgct
     2341 gaaatgcaag cctgcgaact tgcggctcaa gcgtggcgat taggtaaacg ggtactgatt
     2401 gcatgcgaaa ccgagcaaca agcgttaaat attgacgaag cactttgggc aagagatccg
     2461 gattcgttcg tgccgcacaa tttatcggga gaaatcacca cttacccaac gcctatcgaa
     2521 atttcgtgga aaggtaaacg caatgctcaa cgtcgggatt tgttgatttc actccaaacc
     2581 caactccccg aatttatcgg cagttttacc cagattattg atttcgtgcc ggtggaagaa
     2641 accgccaaag cacaggctcg tgaccgctat aaacaatacc gccaacttgg ttggcaactg
     2701 gaaacgatac aatgctaaaa aacctctctt ggcgcgcaaa acgctttgat gaactctcca
     2761 cccaagagtt gtttgagatt taccacgcac gtacagcggt gtttgtggtt gagcaaaact
     2821 gcccttacca agaagtcgat aataaagacc tgcaagcggt gcatttcttt acaaaaaatg
     2881 caaaaaatct gaccgcttac tgccgtttaa ttccagccaa tgatggtgtg catataggta
     2941 gagtgttggt ggtgaaagaa aatcgtggct caggcttagc aagggaattg gtacaaaaag
     3001 cgatggatta ttgtggcgaa cattttcctg ctcaacccat tcacgcccaa gcccaaagtt
     3061 acctgcaagg gttttatgaa agttttgggt ttaaagcggt ttcagaagtg tatttggaag
     3121 atgggattgc gcatttggat atggtgttag aatgaataaa ttagctaaaa tcgaacaatt
     3181 aaaaacagca ctttcacaac gccgtccatt atctgaaagc gaagttcaac gactgcggga
     3241 agagtttatt attgaaagca gctataattc caatgcaatt gaaggcaata ccattacttt
     3301 gcgtgaaacg gtgctcattt taaaagaggg aatgaccatt gccgaaaaac cgattcggga
     3361 gcatttagat attatcggtt ttaaggatgc attcaactat atttatgaat tagtcgccaa
     3421 taaagaacca ttaagcgaac gagcgattaa agatattcat tctctggtat taatgggcaa
     3481 tgcggaaagc cgaggggttt atcgcaaaat tccagtacga attttagggg cagaaaatgc
     3541 cccccccctt atttaattgc cgaagaaatg caattattga ttgaaaaata tcaacaaaaa
     3601 ctgaccgcac ttcatcccat agaggcaatc agttggctgc atttagcctt tgaaagcatt
     3661 caccctttta ttgatggaaa cgggctcaca gggcgattac tgattaattt tgaattatta
     3721 aaacagggct atttaccgat tgatattaaa tttaccaatc gagcccaata ttaccaatgt
     3781 tttgatgatt tccataaaaa tcaacaagat attagtagtt tggttgaatt gattagtcag
     3841 tatgaattag ctgagttgga aaggtatttg gcgattctga aacagaggac ataaaatgac
     3901 aagtcgtgaa gaattactga aaaaacaacg tgaactggat attctcttta cagcatggtt
     3961 tgaagaaaag aaaaagcacg aagtgctgac gtatcgccga gaaaatgggg atttaattca
     4021 gcattatccg gacggtacgg aaaaagtgat tgaatatgca caataaccta gcaatttttt
     4081 actgtggcac aaacggagcc ggcaaatcta ctttacgttc tttcaatcaa gataaagtcg
     4141 agattgtgat tgattccgat aatattgcga tgcagattaa cccactcaat ccaagattgg
     4201 ccgatattga ggcgggcaga aaagcaatcg aactatttca gtttgcagta aaaaatcagg
     4261 ttgcattttc aatggaatca accctctcgg gtaaatctat tctaaaacga atggaaaatg
     4321 ccaaagcagg atatttcaaa actcgattaa attatattgg tgtcgatagc tataaaatta
     4381 acatcgcacg ggtaaaagca agagtgaaag ccggcggaca ttttattgat gaagaaacca
     4441 ttagaaaacg ctatcacatt agccgtgaaa atttaattga agcgattttt atcaatgatg
     4501 aaacactgat ttatgataat tcagaaacca agccgaattt gattttgcag attgctgata
     4561 agaaagtgat aaaaatttca gacaaaatac cgaattggtg taatcagctt tgtgatgaat
     4621 tagtggaatt agggtttaaa accacctagc acgagccgtg ctaggctcga caatcccagc
     4681 tcctttggag ctgtaactta gagccacaaa gtggctcagc tatgcgtaac cccacacggc
     4741 ttgtgtgtgg gtgacaagct gtcggattaa agaaaaactt tgcaaaaaac ttctcgaaac
     4801 agaccgctta tgcctatctc tgataggttc ttaaataaat aagctctcgt cgaagacgat
     4861 caccatcggg gatattggaa caaaaacaca aagatgaaac agaataggaa aaagtctaaa
     4921 aaagttattg gtactcata
//
LOCUS       DANLXQ010000170         4930 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.171, whole genome shotgun sequence.
ACCESSION   DANLXQ010000170 DANLXQ010000000
VERSION     DANLXQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.171"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(100..1416)
                     /gene="argA"
                     /locus_tag="PXD94_002315"
     CDS             complement(100..1416)
                     /gene="argA"
                     /locus_tag="PXD94_002315"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL6287130.1"
                     /translation="MQNTELALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNI
                     INDISLLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGK
                     VHYDFFARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYTLSGKIRRINIESIKQQL
                     AQNSIVLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIP
                     DYLPQDGETLLQQLIERGEYHTPKARFLQAANTVCREGVKRSHLLSYKEDGSLLQELF
                     SRDGIGTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTI
                     IERDGVVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKL
                     FVLTTRTTQWFQERGFRLAGAQDLPRYKRENYNYQRRSKVLILPLW"
     gene            complement(1545..1730)
                     /locus_tag="PXD94_002316"
     CDS             complement(1545..1730)
                     /locus_tag="PXD94_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HDL6287131.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATNQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
     gene            complement(1846..3168)
                     /gene="pepP"
                     /locus_tag="PXD94_002317"
     CDS             complement(1846..3168)
                     /gene="pepP"
                     /locus_tag="PXD94_002317"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL6287132.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            3384..3707
                     /locus_tag="PXD94_002318"
     CDS             3384..3707
                     /locus_tag="PXD94_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6287133.1"
                     /translation="MKLSSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFG
                     TQLNAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAIN
                     THKQE"
     gene            complement(3784..4802)
                     /gene="glpX"
                     /locus_tag="PXD94_002319"
                     /pseudo
     CDS             complement(3784..4802)
                     /gene="glpX"
                     /locus_tag="PXD94_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
BASE COUNT         1463 a         1118 c          855 g         1494 t
ORIGIN      
        1 cagtaataat tgccatagcc cctttgatgg gttgatgttt tttcatagtt ctccttttat
       61 agttctcttg gtaacaagcg gtcaaaaaca ttgaatattt taccaaagtg gtaaaattaa
      121 tactttagaa cgacgttgat aattgtaatt ttcccgttta tagcgaggta aatcttgggc
      181 acctgccagc ctaaatcctc gctcttggaa ccattgtgtg gttcttgtag tcagcacaaa
      241 gagtttctcc attcccagtt gcactgcccg tttttgaata ttttccagta gaatatcccc
      301 tcttgaggaa tcacgataat cagggtgaac cgccacacac gccatttccg ccattttttc
      361 ttccggatac gggttaagtg ccgcacaagc aatcaccaca ccatcacgct caataatggt
      421 gtagtttgtg atttccattt ctaattgttc tcgagagcgt ttgactaaaa tgccctgctg
      481 ttcaagtggg cggattaaat ccaataatcc cggaatatca ctggaattag caatacgtac
      541 ctgttccgag ctttccattg agaactgtgt gccaatacca tcacgagaga ataactcttg
      601 aagcaatgag ccatcttctt tatagcttaa taaatgcgag cgtttcactc cctctcggca
      661 taccgtgttc gccgcctgca aaaaccgagc tttcggggtg tgatattcgc cacgctcgat
      721 taactgctgt aacaaggttt cgccatcttg cggaagataa tctggaattg gcttgccgtt
      781 ttcatctaaa atcccctgct cttcacaaaa accgattaat ttatcggcac gaagtttaat
      841 tgcaacctgt gtggcaatat cttcaaacgg cagattaaac atttcaccgg taacagacgg
      901 ggcaatcgga ccaagtaata caatcgaatt ttgtgccagt tgctgcttaa tgctctcaat
      961 attaatacga cggattttac cgcttagtgt ataatctacc ccatccacca cgccaatcgg
     1021 ctgagctaaa atcatattac cactgactac attaatcact tcagaatggg gtaaacgcaa
     1081 agaaaggcgg gcaaagaaat cataatgaac tttccctacc gcttgtttca catattctag
     1141 cgattgtgga ttagtcacac ggatattgtg gtaatactcc ggctcaatat gattttgttt
     1201 taacaattgg ttaatttgcg gtcttgcccc aaatacaatc actaatttga tattcaggct
     1261 gtgcaggaga ctgatgtcat tgataatatt gacaaaattc gggctttcga taacatcgcc
     1321 atcaagcata atcacaaagg ttttgcctct gtgcatatta acataaggtg tagattgcct
     1381 aaaccattga gctaatgcga gttctgtgtt ttgcatatat ttcctcttta taatataaac
     1441 gcatattatc ttttttagaa aaatccgcaa gccttaaatt gaataattaa gcattatatt
     1501 tgcataaata acaaagccct gaaataatat cagggcttta tagattatgc ttcagctttg
     1561 gttaaatctt ttacttctgt ttcaggtgta acttttttaa ctgcttcaat gcctttttga
     1621 caaccttgct tagtcgtata agcttggttg gttgcaataa cttcgtggtt acccgctttt
     1681 aaacgccaac gaaattcttc ttttgcatct ttgtaaattt caaaatacat ataaacactc
     1741 cttcgtgttg tgattgccca aacactgtgt ttgtgtcggg ttagcttaag ctaaattccc
     1801 caaaatatca aagcaagcgg tcattttttt acaaaaattt gcgaattaat ttaacgattg
     1861 catcaactgc tcaacatccg caatttcttt agggcaaccg ctgcttaagt ttttgttgcc
     1921 gtattcggta atcagcaaat tatcttcaat ccgaatccca atacctttat attgctccgg
     1981 tacatccgca tctttaggaa tatagatacc gggttcaaca gttaacacca taccaatctg
     2041 caaaggacga tcccgctccg tgccgtaatc ccccacatcg tgaacatcta gccctagcca
     2101 atgccctaat ccgtgcatat aaaattggcg ataggctttt tgctcaatca ggctttccac
     2161 atcaccgttt aaaattccta agcgaaccaa gccctgcgtg agaatctgta cagccttatc
     2221 gtttgccgct ttaatcgagc tgtttggcac aagaagatta atcgcctctt tttgcatagt
     2281 taaggttaat tcgtacagct ctttttgcgg ttcggagaat ttcccattaa tagggaaggt
     2341 tcgggtaata tcgccggcgt aatacgcaaa ttccgcccct gcatcaatca gtaataaatc
     2401 gccgccttta agcacctgat cattttcatt gtagtgaaga atacaggcat tctcaccgcc
     2461 ggcaacgatt gaattatatg ccggaaacct tgctccaaaa cggctaaatt cgtgttgaat
     2521 ttccccctca atttcccact cataacgatt cgggcgagct tgtttcatcg ctcgaatatg
     2581 tgccattgaa gaaatatgac acgcctgctg aattaaggca atttcaaatt ctgatttaat
     2641 caaccgcatt tccgataaca ttggctgcca atcaatcaag gttgtcggca ctttttgccg
     2701 attgactttc attttttcaa atgttgcaaa caaaatatta tcgccccact cttgtatccc
     2761 taacgcacaa taacaagcgg tcagattttc taattttttt gcaagaacgg tttcaatgtc
     2821 atcaatatca aaagctgcgt taagctgtaa cgtttttgga gcttcctcaa tccctaaacg
     2881 tctgccattc caggtttcca ttaaagggtc ttttttacgc acaaaaataa tactttccgt
     2941 tttaccttcg ctttttacca gcaatacggc tgatttaggt tctgccaaac cggttaaata
     3001 ccaaaagtaa ctatcaggtc taaacaagta ttcagagtca ctattacggc gtttttccga
     3061 ttcggtaaaa accaataacg cagaattatc ctgcatttgc tcaagcacac gctcacggcg
     3121 tgcggtaaat tcttcttgtg gcattttagc caaataagcg agatccatta tttctccttt
     3181 ttattatatc tatatcgaaa cctgccttat tttagcacaa acgcagcata aattcagcct
     3241 aaccaaaaca gatgaaagat tatatttgag atcttgattg caaaaacaaa acagattaag
     3301 attaaatata acctttacat atctaaacag gttatattta acctatgaac acataaataa
     3361 tctactttta gctcaaaaat aaaatgaaac tatcaagcga actacaaaat cagataaacc
     3421 aactaaatca gttacgaaaa caggcactca aacaagagca agacgagcag aaattgtttg
     3481 aaaaaaataa aattgaagat ttgccacatt ttggcacgca gcttaatgct cagaggaaac
     3541 tattaggcat agaattaacc acccttgaaa tgcaaaccgg catttcaagc tctaccctaa
     3601 aacgcttatt taaagaccca agccaagtga aatttcagac aatttgtatt gtggcagaaa
     3661 cactcggctt taatttatgt gcaattaata ctcacaaaca agagtaagcg tgacaatgta
     3721 gtgattttga ttactttggg aagacaaaaa atcgggctaa aagcccgatt tatcagagaa
     3781 aagctataaa tccaataaag aaaggagttc gttatcttta cgatcgacat aatggtcgga
     3841 ttgaatttta cgaatggttc tagatttgcc acgaataagt aaggtttcag tgctggcaat
     3901 attgcctcta cgtttaatcc cttttaacaa ttcacctgaa gtaatgccgg ttgcaacaaa
     3961 gacgatattg tcatcacgca ccaaatcttc taatttcaac accgtattca ccggtacatt
     4021 catttcatta cagcgtaaaa gctctttttc ggtagcagtt aaattttccg ggctattccc
     4081 cttcacttgg ttgcgaggaa ttaaacgtgc ttgcatatcg ccacccaatg cacgaaccgc
     4141 agcccccgcc actacccctt caggtgctcc gccgatacca taaagcagat ccaattcgct
     4201 ctctggcaaa cagcattgca ccgcagccgc aacatcgcca tcaggaatcg caatcactct
     4261 tacccctaat ttttgcacat cggcaattat tttttcgtgg cgaggcttag caagatagca
     4321 atggttaatt gggaaagtaa tttgcccttt ttcgaggcga cacggcgaag attttgttca
     4381 agcggaagat ttaaatcaat cattcctttg cattcctgcc ccaccactaa tttttccata
     4441 tacatatcag gggcttttaa aaaggtttct ttgccgcctg ctgctaatac cgacaacgca
     4501 tttgcctgcc ccattgcagt catacgagta ccgtcaatcg ggtcaaccgc aatactgatt
     4561 tcctcatctt ctaggcggga tagaccgatt ttctcgccaa tataaagcat cggtgcttcg
     4621 tcaatttcgc cctcaccaat taccacttcg ccacggattt ccatttggtt cagcataaac
     4681 cgcattgctt tcaccgcagc ttcatcagcg gcatttttat ccccacggcc tagccaagag
     4741 taagctgcta gagccgctgc ttccgtaaca cgagaaaatt caaaagaaag agttcgtttc
     4801 atttttgttc cttagtttct tgaaagaaaa actttaaaag aagagagatt atattttatc
     4861 agtaacctgc aagctacgtt actaaaaaat cagcgtttga ataaaaattc acaaatagcc
     4921 aaactttttt
//
LOCUS       DANLXQ010000171         4914 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.172, whole genome shotgun sequence.
ACCESSION   DANLXQ010000171 DANLXQ010000000
VERSION     DANLXQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.172"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            83..568
                     /locus_tag="PXD94_002320"
     CDS             83..568
                     /locus_tag="PXD94_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL6287134.1"
                     /translation="MKIKTLSLIMLLSVTVNAAPVELHQFDSPKQESDYRALIQELRC
                     PQCQNNNIADSNATIATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPAT
                     IMLWLLPLILIALGLGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKEE
                     K"
     gene            565..1476
                     /gene="ccmI"
                     /locus_tag="PXD94_002321"
     CDS             565..1476
                     /gene="ccmI"
                     /locus_tag="PXD94_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL6287135.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPSKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1473..2078
                     /gene="mog"
                     /locus_tag="PXD94_002322"
     CDS             1473..2078
                     /gene="mog"
                     /locus_tag="PXD94_002322"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL6287136.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLAVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            2088..2426
                     /gene="glnB"
                     /locus_tag="PXD94_002323"
     CDS             2088..2426
                     /gene="glnB"
                     /locus_tag="PXD94_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL6287137.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2507..2986
                     /gene="lspA"
                     /locus_tag="PXD94_002324"
     CDS             2507..2986
                     /gene="lspA"
                     /locus_tag="PXD94_002324"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL6287138.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AIGNAIDRAYNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKKEPKK"
     gene            2997..3941
                     /gene="ispH"
                     /locus_tag="PXD94_002325"
                     /gene_synonym="lytB"
     CDS             2997..3941
                     /gene="ispH"
                     /locus_tag="PXD94_002325"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL6287139.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            3941..4189
                     /locus_tag="PXD94_002326"
     CDS             3941..4189
                     /locus_tag="PXD94_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL6287140.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            complement(4396..>4914)
                     /locus_tag="PXD94_002327"
     CDS             complement(4396..>4914)
                     /locus_tag="PXD94_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6287141.1"
                     /translation="QSANQYIFNSDWFTHNVPSLASIFEYVKPRRILEIGSFEGRSSV
                     FFIEEALKYQSQVEIHCIDSWEGGAEHQGVWDMGEIEQRFIHNVTLAQQKFQNVVLYK
                     HRGYSHQKMIELLANGYINYFDYIYIDGSHEAPDVLFDALLAHRLVRKGGVISFDDYL
                     WSPDETGKQRHY"
BASE COUNT         1565 a          988 c         1050 g         1311 t
ORIGIN      
        1 gaaaacacag aaacttttgt actggaaatt gaaaataaca aatatcaaat tcctaataaa
       61 tatatcaaca atattcaaaa ttatgaaaat aaaaactctc agcctaataa tgcttctaag
      121 tgtaacagtt aatgctgctc cggtagaact gcaccaattc gacagcccga aacaagagtc
      181 ggactatcgt gccttaattc aagaactgcg ttgcccgcag tgtcaaaata ataatatcgc
      241 cgattccaat gcgactattg ccaccgatat gcgggcaaaa accttagaat tgctcaaaca
      301 aggcaaaagc aaagacgaag tggtggatta tatgattgaa cgctacggca atttcgtcac
      361 ttacgacccg cctctcacac cggcaaccat tatgttatgg ctgttgccgt taattttaat
      421 tgccttaggg ctgggggtgg tgttgcgtag aaagaaaaac aaaccacaag cggtcaattt
      481 tgagcaaaat actgcaaaac cgaccactca tctcgctcaa gatgagcaag aacgtcttaa
      541 ccaaattctc aataaggaag aaaaatgaat ttctggctgg caattatttt cgttacactg
      601 attgtatgtg gcgtggcgtt ttatccgtta cttaaaaaga acaagcaaat cgataacacc
      661 aagcgagaca gcttaaacaa agccttctat tttgaccgct taaaagaagt ggaaaacgaa
      721 gcgaatgaag gggtgattga tgaccctgaa caaaccaaat tagagctaca aaaaaacttg
      781 cttgatgata ttccacacag cacacaaagt gccacaagtg aaaaattaac gctcggcaag
      841 tcgtggtttg tggcattgat tttatttgtc ggcggtatca gctttgcgat ttacagcaat
      901 gtcggttctt ggttctccaa ctcaatgtta gacaccactc accaaaaatt agacagcttc
      961 tatgagcgtt taaaaaccga agagaccaat ccgctctcac aagaggaaat gaaccaattt
     1021 gcgatggcat tacgggtaga tttacaaagt aatccgaatg acgacaaaaa atggtatatg
     1081 ctcggtcaaa tcgggatggc gttagatgat ggtcagcttg cacacgatgc ctttagcaaa
     1141 gcggctcaac tcaagccgga taacttagat tacaaactca gccacgccca actgctgctc
     1201 ttttccgaag cacaggaaga caaaaaacag ggtgaaacct tgttaaaaga gattatccgc
     1261 caagaccaca caaactttaa tgcgttaagt ttattggcgt tccactcctt tgaaaaagaa
     1321 gatttcaaaa tggcggcaat gacgtgggga atgatgttaa aactactgcc tgatgaagat
     1381 cctagcaaag taacggtaga acgcagtttt aatatggcgt tatccaattt aagtgaggcg
     1441 gaaaaacaag aattgcaaaa aggtgagaaa aaatgaccgc ttgtaaacca cttagcaaag
     1501 agaagttgaa aatcggcttg gtgtcggttt cagaccgagc ctcgcagggg gtttattcag
     1561 accaaggcat tcccgaatta agggcgtggc ttgaatctgc cttaaccgag ccgtttgagc
     1621 tggaaacccg cttaattccc gatgagcaag ccattattga gcaaacctta atcgagttag
     1681 tcgatgctca cgcttgccat ttggtattaa ccaccggcgg caccggcccg gcaaaacgtg
     1741 atgtcacccc agatgccacc cttgctgtcg cccaccgaga aatgcccggc tttggcgaac
     1801 agatgcgaca agtgagcctt cacttcgtgc caaccgccat tctctctcgc caagtgggag
     1861 taattcgcca cgacagtctg attttaaatc tgccggggca accgaaagca attaaagaaa
     1921 cgctggaagg tgtgaaagat gaacagggca aagtattggt taaaggtatt tttagtgccg
     1981 tgccatactg tttacaactt ttaagcgaaa tttatgtcga aacccacgca gctatttgcg
     2041 aaagtttcag acccaaatcg gcaagacgag aaaattaagg ctaaaaaatg aaaaaaatag
     2101 aagcaattat taaacctttt aaattagacg atgtgcgtga agccttaacc gatgtgggtg
     2161 tcagcggaat gagtattacc gaaatcaaag ggtttggcag acaaaaagga cataccgagc
     2221 tttatcgtgg ggcggaatat gcgattgatt ttctaccgaa agtgaaagtc gaaatcgtga
     2281 ttcccgacga gttggaagaa cagtgcattg aagcaattat ggaaaccgcc caaaccggca
     2341 aaatcggcga tggcaagatt tttgtgtatg atgtcgggcg ggtgatccgc attcgtaccg
     2401 gcgaagaaaa tgaagaagcg atttagtttt gcaaaaaatc cggcaaaaac gaccgcttgc
     2461 agggggattt ccatctactc tttttacatt aaattttgga tattaaatga aaacaggctt
     2521 aaaatttctt tgggtgagtt taattgtaat catcattgac ctttggtcaa aatacattgt
     2581 agtgcaacat tttgaatttg gtgaaagcat taatattttg ccgattttta acttcactta
     2641 cgcccgaaac tacggggctg cctttagttt ccttgccgac cattccggct ggcaaaaata
     2701 tttcttttta gggctggcgg tagtgatttc cagtgcttta gtggtggctc tttggaagaa
     2761 tcaagcggtc aaaaaattag aaaatattgc gtatgcctta attattggtg gagcgattgg
     2821 gaatgccatt gaccgtgctt acaacggcta tgtagttgat ttccttcatt tttattggga
     2881 tatttatcac taccctgtgt ttaatattgc cgatattgcg atttgtgtgg gtgcaggctt
     2941 actgatttta gaagccttta taaccaaaaa agaaccgaaa aaataagagt gattaaatga
     3001 aaattttatt agcaaaccca cgcggttttt gtgccggcgt ggatcgagcc atttcgattg
     3061 ttgaattagc cttagaaatt cacggtgcac ctatctatgt tcgccacgaa gtggtacata
     3121 acaaatttgt ggtggatggc ttgaaagcca aaggggctat ttttgttgag gagctacacg
     3181 aagtgccgga cgatgctatc gtgatcttct cagcccacgg ggtgtcgcaa gtagttcgcc
     3241 aagaagccaa aaatcgtggc ttaaaagtgt ttgatgccac ttgcccactc gtgaccaaag
     3301 tgcatatgca ggttgcccga gccagtaaaa aagggacaaa agcaatttta atcggtcacg
     3361 aaggtcaccc tgaagttatc ggcacaatgg gacaatacaa taaccaagaa ggtggcattt
     3421 atttggtaga atcggtggaa gatattgcca acttgccggt ttccaacaac gatgaattaa
     3481 cttttatgac gcaaaccaca ctttccattg acgataccat tggcgtgatt gaggcgttaa
     3541 aagagaaata tccggcaatt caaggccctc gcaaaaatga tatttgttat gctaccacca
     3601 atcgccaaca cgcagtgcgg gaactggcag aacaatcaca attagtgtta gtggtaggtt
     3661 caaaaaactc ctcgaactca aatcgtttgg cggaactcgc ctctcgaatg ggtgtgatgt
     3721 caaaactgat tgatggtccg caagatattg atccaacttg gttggagggt attgaaacta
     3781 tcggtattac cgccggtgct tccgctcctg aagtgttggt gcaatcagta atttcgcatt
     3841 taaaaaccct tggcgtaacc gaagttgaaa ccttagtcgg caacgaagaa gacgtggtgt
     3901 ttgaagtccc gaaagagtta cgcatcaaag aggtaaaata atgacagaaa tcaaccgctt
     3961 taaattagaa tgggcgtgcc gacgtggtat gcgtgagtta gacaaaatga ttatgccgtt
     4021 ttaccaaaat cattttaatg aactcaatga aacccaacag caagctttcg ttgaaatgct
     4081 caattacagc gacccggaac tgtttcgctg ggtaatgctg caagcacctg ccccaaccga
     4141 agaaatcgct gaattgatta ggttaatgcg agataaaatt gagacctaga gctaatagat
     4201 taacctcttc tttggggtaa atatatagct acttacccca aagaagacta aattcattca
     4261 acttttacaa aataacccta aactagaaaa gttaatcttc caatttaata atatgttgct
     4321 gataagctat taaaccttgt attacatgca ctttctgctg ataggtattg acatagtgat
     4381 caacagcggg ttttactaat aatgacgctg tttacctgtc tcatctggtg accaaaggta
     4441 atcatcaaaa ctaataacgc ctccttttct cactaaacga tgtgcaagta aagcatcgaa
     4501 caacacatct ggcgcttcgt gagatccatc tatgtaaata tagtcaaaat aatttatata
     4561 tccatttgcg agaagttcaa tcattttttg gtgagaatag ccacggtgct tatataacac
     4621 aacattttgg aatttctgtt gcgcaagagt gacattatgg ataaatcgtt gttctatttc
     4681 tcccatgtcc caaactcctt gatgctctgc tcccccttcc caactatcaa tacaatgaat
     4741 ttcaacttgt gattgatatt tcaatgcttc ttcaatgaaa aagacagatg atctaccttc
     4801 aaatgatcca atttctaaaa tcctccgagg ttttacatat tcaaaaatag aagctaatga
     4861 aggaacatta tgagtaaacc aatcagagtt aaaaatatat tggttggctg actg
//
LOCUS       DANLXQ010000172         4819 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.173, whole genome shotgun sequence.
ACCESSION   DANLXQ010000172 DANLXQ010000000
VERSION     DANLXQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.173"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(74..640)
                     /locus_tag="PXD94_002328"
     CDS             complement(74..640)
                     /locus_tag="PXD94_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287142.1"
                     /translation="MEGLIMGLQLIPHFPELAISRRTYLHDMPMYCFVSKNWIFSAKD
                     RAKREALGNLLACGGEMMKSFIFKRSLFSEIWFEDILLEDEEKELIRILYNKPEPEYQ
                     VEEKIDWEAREARRKQADYDKWQSYDERNPFHYQIFPTDTIAYAQQQVEKFIEDVDNF
                     LEWFYDDEVKRYILERNEIYQKKFDLVN"
     gene            complement(1173..1499)
                     /locus_tag="PXD94_002329"
     CDS             complement(1173..1499)
                     /locus_tag="PXD94_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6287143.1"
                     /translation="MLHSKGKILPKITLNEIETKERFAIALRKEMHAQYWTGKMVANA
                     LDVSEKTVKDWVAGKRLPSSLDLLALMRISSCVRQLVWDISKSEECYQDAEMLKQICF
                     VLLSNL"
     gene            1547..3178
                     /locus_tag="PXD94_002330"
     CDS             1547..3178
                     /locus_tag="PXD94_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL6287144.1"
                     /translation="MVTRTKNQPKNQAVSYIRMSTEHQEFSPDLQRCFIENYAKENGL
                     EIIREYIDEGKSGLTAEHRPQFLSMIQLVQSGQADFDHILVYDVSRWGRFMNIDESGH
                     YELICTYQGIKVHYCAEPFKGNDIASQIYKTMKRLSVGDYCRELGVKVFNGQKNLIQH
                     GFRQGGVAGFGLRRRLIDCQGNPKFDLQRGDRKSLQTDRVILVAGPKEEQEIVRQIYH
                     DFVYQHKTEQQIADSLNAQGIVTDRNTAWTKGVVHQILINEKYVGHNVWNKRSASKTR
                     EYKGKNPVEEWVRAENVFEAIVPQALFNAAQTIIHQRSARLSDDEMLEQLKLLLKEKG
                     KLSGLIIDEAENCPSSSAYSSRFGSLLNTYTLINYEPERDYHYVEINRLLRQQHKNFV
                     QQTLDTIIELGGVVLSDDVTDLVHINNEFTASIVLSRCRLTPSGSKRWLIRFDTSLNP
                     DITIAVRINESATEILDYYLLPTTEKVNEKLRLAESNPAELEIYRHDNLDRFFIMVER
                     ILVKEFIYAKRHHSNSSYRQNSGTQSQNAQSESLC"
     gene            3093..3986
                     /locus_tag="PXD94_002331"
     CDS             3093..3986
                     /locus_tag="PXD94_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL6287145.1"
                     /translation="MQNDTIQILPIDKIRVLNPRTRNQKVFADLVENIATIGLKRPIT
                     VTPSVDSAEYYDLLCGQGRLEACQVLGELFIPCRVVNANSEESYLISLVENIARRQHS
                     YMELLSGIKILNDRGYKPVDIARKIGFSKSHVNSILHLINAGEERLINAVEQGILPIT
                     IAISISRSDDQEVQKQLAELYTNGTLKSTDIAKIRNLIHRRNLAGKKANTGISGSIRS
                     SIYNPKTVVNIYKEETERQKMMIKQADYDEKQLSIILSCLNKLFEDKYFQLVLKSEHL
                     DDMPKDLSQRMLKQREGNIYA"
     gene            3979..>4819
                     /locus_tag="PXD94_002332"
     CDS             3979..>4819
                     /locus_tag="PXD94_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL6287146.1"
                     /translation="MPKHITYAFHNQLISLPIDEIIPLKKMDKNFEKSTKFQSILASI
                     REIGIVEPVVVYPEENNQYILLDGHSRVHALKKLGVDKVLCMISTDDEGFTYNKQINR
                     LTAVQENKMLMKTIERGVSDELIARTLNINLKALRQKMNMLNGITREVVDILANKQVS
                     KEIFRILRKMKPERQIEVAQMMIASNKFTLTYANMMLLSSRKEELVESHKSKTTNDDL
                     TDLSIMQKELERLKENYKISEEKLADLKMGLVVAKGYVKKLLNNAAVVDLLENDQSEI
                     YLTL"
BASE COUNT         1588 a          924 c          933 g         1374 t
ORIGIN      
        1 aatctacaaa tatcaagtgt aaaaaaatat aaaagtcggc aataacatta ttttataata
       61 actaatgtga attctagttc actaaatcga atttcttttg gtaaatctca ttcctttcta
      121 atatatagcg ttttacctca tcatcgtaaa accattcaag aaagttatca acatcttcaa
      181 tgaacttttc tacctgttgc tgtgcatatg ctatcgtatc ggtagggaag atttgataat
      241 gaaacggatt gcgttcatca taactctgcc atttgtcata atcggcttgt ttacgccgcg
      301 cttctcttgc ctcccagtcg attttttctt ccacttgata ttctggctct ggcttattat
      361 acaaaatacg gatgagttcc ttttcttcat cctcaagcag aatatcttca aaccagattt
      421 cagaaaatag gctgcgcttg aaaatgaaag acttcatcat ttcccctcca cacgccaata
      481 agtttcccaa tgcttcccgt ttcgctctat cttttgccga aaatatccag tttttcgata
      541 caaaacagta cattggcatg tcatgcaaat aggttcttcg tgaaatggct aattcaggaa
      601 aatgaggaat aagctgtaac cccattatta agccttccat actgcacacg atcttactta
      661 attgtccgta atcgcgtaaa aaagcaatgt cagtttttat caaccacaac gataggctga
      721 ctaatatgaa cataaagatg agataataga tcggttttaa ggctaaggcg cgccgacaaa
      781 gccacattaa gagtggaatc gtaacgagat caaagaaaaa tgatgagtct atgatgattt
      841 ccttatgtga gttgatgaat aatcaggcat gaaggaaacc ttaaaaagga aaagtccctt
      901 cattaccact ttttaaaacc ttttaaggct taaaaatcaa aagtcgtaat gaagggacta
      961 ttgtccgtgg gcaaaaaggt attagttata gggggctttg tcaagcgaaa atttactgtg
     1021 cattttctcg ccaaaactga tttaagcctt gataaaatct cgaaagtacc ttgtcaagaa
     1081 aaatcttttt agtggtttga ttgctagttt tttgagcaaa actatgctgt aatgcagtaa
     1141 aaatacagtt tctatctgtg tttggaatcc agttacaaat tggagagtaa tacaaagcat
     1201 atttgcttga gcatctcagc atcttggtag cattcttctg attttgaaat atcccaaaca
     1261 agctgccgta cacatgatga aattctcatt aacgccaata aatctaggct acttggcaat
     1321 cttttccctg caacccaatc tttaactgtt ttctcactca catctaatgc attggcgacc
     1381 attttccccg tccaatattg agcatgcatt tcctttcgta acgcaattgc aaaccgctct
     1441 ttcgtttcaa tctcattgag tgtaattttg ggtaagattt taccctttga gtgcaacata
     1501 tttcctcccg attcctttat attggtgtag ataaggagtc attagaatgg ttacacgtac
     1561 aaagaatcaa cctaaaaatc aggcggtttc ctacattcga atgtcaacgg aacaccaaga
     1621 attttcacca gaccttcaac gttgctttat cgaaaattat gctaaagaaa atgggctaga
     1681 aatcattaga gaatacattg atgaaggaaa aagtgggcta accgcagaac accgcccaca
     1741 attcctttca atgatacaac tcgtacaaag tggacaagcc gacttcgatc atattttggt
     1801 gtatgacgtg agtcgttggg gtcggtttat gaatattgat gaatcggggc attatgaact
     1861 gatttgtacc taccaaggca ttaaagtcca ctattgcgcc gagccattca aagggaatga
     1921 tattgcctct caaatatata aaaccatgaa acgtttgtcg gtaggagact actgtcgtga
     1981 actcggagtg aaagtattta acggtcaaaa aaatctgatc caacatggat ttagacaagg
     2041 tggggtagct ggatttggat tgcgtaggag attgattgac tgtcaaggaa accctaaatt
     2101 tgatctccaa cgtggagata gaaagagcct acaaaccgac agagtgattt tggttgcagg
     2161 cccaaaggaa gagcaggaaa ttgtccgaca aatttaccat gattttgttt atcaacataa
     2221 aacggaacaa caaattgctg atagcttaaa tgcacaaggc attgttactg atagaaatac
     2281 agcatggacg aaaggcgtag tacatcaaat ccttattaac gaaaagtatg tcggccacaa
     2341 tgtttggaat aaacgtagtg catcaaaaac acgcgaatat aaaggtaaaa accccgtaga
     2401 agagtgggtg cgagctgaga atgtatttga agctatcgtg ccacaagcct tatttaatgc
     2461 agcccaaacc attattcatc aacgctccgc ccgattatct gacgatgaaa tgttagagca
     2521 actcaagcta cttttgaaag agaaagggaa gctgtctggc ttgatcattg atgaagccga
     2581 aaattgtcca tcaagtagcg cgtacagtag ccgatttgga agcctactta acacatatac
     2641 tttaattaac tatgaacccg aacgggatta ccactatgta gagatcaacc gattactccg
     2701 tcagcagcat aaaaatttcg tacaacaaac acttgatacc attattgaat tggggggagt
     2761 ggttttgagc gatgatgtaa ccgatttagt ccacatcaat aatgaattta ccgcctcaat
     2821 cgtattaagt cgttgtcgcc taacgccatc aggaagcaaa agatggttga tccgatttga
     2881 taccagttta aatcccgaca taacaattgc agtgcgcatc aatgagagtg ccacagaaat
     2941 tcttgattac tacctgcttc ccacaactga aaaggtaaat gaaaaactac gattggctga
     3001 gagtaatcct gccgaacttg aaatctaccg ccatgataat ttagatcgct tttttattat
     3061 ggttgaacgt attttagtca aggagtttat ctatgcaaaa cgacaccatt caaattcttc
     3121 ctatcgacaa aattcgggta ctcaatccca gaacgcgcaa tcagaaagtc tttgctgatc
     3181 tggtcgaaaa cattgcaacg attggtctca agagacctat taccgtaacg ccatctgtcg
     3241 atagtgctga atattatgat ttgttatgcg gtcaaggtcg gcttgaggct tgtcaggtgc
     3301 ttggtgagct attcatacct tgtcgcgttg tgaacgcaaa tagtgaagaa agctacttga
     3361 tcagccttgt ggaaaatatt gccagacgtc agcattccta tatggagctg ctttcaggca
     3421 tcaaaatact caatgataga ggttataagc ccgttgatat tgctcgcaaa atcggtttct
     3481 caaaaagcca tgtcaacagt atcttacacc tcatcaatgc tggggaagag cgccttatca
     3541 atgcggtaga gcaaggaatt ttacccatta ccatcgctat ttcgatttct cgttcagacg
     3601 atcaggaagt tcaaaaacaa ctcgcggaac tttacaccaa tggtacattg aaaagcaccg
     3661 atattgccaa aatacgtaac ctgatccacc gtagaaatct tgctggtaag aaagccaata
     3721 caggtatttc tggaagtatt agatcatcta tttataatcc caaaactgtg gtgaatatct
     3781 acaaagagga aacggaaaga cagaaaatga tgatcaagca agcggactat gatgaaaaac
     3841 aactctctat tatcttgtct tgcttaaata aactctttga ggacaaatac tttcaactcg
     3901 tccttaaatc agaacattta gacgatatgc caaaagattt aagccaacga atgttaaaac
     3961 aacgagaggg aaatatttat gcctaaacat attacttatg cctttcataa tcagttaatt
     4021 tcgcttccaa ttgatgagat tattccatta aaaaagatgg ataaaaattt tgagaaaagc
     4081 actaagttcc aaagcattct ggcttcaatt cgtgaaatcg ggattgttga acctgtggtc
     4141 gtctatccag aagaaaacaa ccaatatatt ttgttagatg gccattctcg tgtacatgca
     4201 ctcaaaaagc taggtgtgga taaggtgctt tgtatgattt ctaccgatga tgaaggcttt
     4261 acttacaaca agcaaattaa ccgcttaacc gccgttcagg aaaataaaat gctgatgaaa
     4321 accattgagc gcggggtttc ggatgaactg atcgcaagaa cactcaatat caatctaaaa
     4381 gcactacggc agaaaatgaa tatgcttaat gggatcacaa gagaggttgt tgatatattg
     4441 gccaataagc aggtcagtaa agagatattt cggattttgc gcaagatgaa gcctgaacgc
     4501 caaattgaag tggcacaaat gatgatcgcc agtaataagt ttacattgac atacgccaat
     4561 atgatgttgc tcagttcacg caaggaggaa ttagtcgaaa gccacaagtc taaaaccacg
     4621 aatgatgatt taaccgatct ttccattatg caaaaagagc tagagcgact taaagagaac
     4681 tataaaattt ccgaagagaa attggcggac ttaaaaatgg gattggttgt ggcgaaaggg
     4741 tatgtcaaaa aactattgaa taatgctgcc gtggtagatt tgcttgaaaa tgatcagagt
     4801 gaaatctatc ttactctta
//
LOCUS       DANLXQ010000173         4698 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.175, whole genome shotgun sequence.
ACCESSION   DANLXQ010000173 DANLXQ010000000
VERSION     DANLXQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.175"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            26..355
                     /gene="trxA"
                     /locus_tag="PXD94_002333"
     CDS             26..355
                     /gene="trxA"
                     /locus_tag="PXD94_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL6287147.1"
                     /translation="MSNVIHTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDD
                     LSVDAQFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMA
                     EFIKQAL"
     gene            526..1749
                     /gene="hflK"
                     /locus_tag="PXD94_002334"
     CDS             526..1749
                     /gene="hflK"
                     /locus_tag="PXD94_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL6287148.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPTNNQRNEQ
                     QPPDLEEVFSSLLKKMGGGKGPNNSSNQPSASLGKFLPVVLGLAAVVWVGSGFYTVQE
                     AERGVVTRLGKLNDIVLPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            1752..2639
                     /gene="hflC"
                     /locus_tag="PXD94_002335"
     CDS             1752..2639
                     /gene="hflC"
                     /locus_tag="PXD94_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL6287149.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MMLLKPNSEFLRFMQAPTK"
     gene            2813..4111
                     /gene="purA"
                     /locus_tag="PXD94_002336"
     CDS             2813..4111
                     /gene="purA"
                     /locus_tag="PXD94_002336"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL6287150.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
     gene            complement(4271..4341)
                     /locus_tag="PXD94_002337"
     tRNA            complement(4271..4341)
                     /locus_tag="PXD94_002337"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4311..4313),aa:Arg,seq:tct)
     gene            complement(4490..>4698)
                     /locus_tag="PXD94_002338"
     CDS             complement(4490..>4698)
                     /locus_tag="PXD94_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287151.1"
                     /translation="YVVGTGDDTDGEVTNFVIITSEYKYIDAQSAKEITDAPLTKEDI
                     GKTPTQIMLGRIYKYLKETGQIVV"
BASE COUNT         1444 a          846 c         1077 g         1331 t
ORIGIN      
        1 ttatttaata tttaaggata aacttatgag caatgtcatt catacaacag atgctacatt
       61 tgaacaagat gttttaaaat cagatgtacc ggtattatta gacttctggg cgccttggtg
      121 tggtccttgc cgtatgatcg gccccgtact tgacgattta tctgttgatg cacaatttga
      181 tggtaaagta aaaattgtca aaatgaacgt agatgaaaat ccatctgtac cagcacaatt
      241 tggcgtacgc agcattccat ttttactatt attcaaaaat ggtgaagtga ttgcccagca
      301 agttggtgca attccgcacg cccaaatggc tgaatttatc aaacaagcat tataatttta
      361 agagcaatac tagggcgaat ttatcgccct atttttttgc aaataaatag tgaaaaaaga
      421 ccgcttgttt cagataaacc tagtcaaaag cttactaaat aggtacaatc actaacttgc
      481 attctaagct ttaaagtgcg attcgatttt attttggaga tatttatgtc gtggaatgaa
      541 tcaggcaatc agcaagatcc ttggggtaaa ccggggcaga aaaaagctga gccaaagcct
      601 gagcaacaag aacagggttc acaacaagag ccgaccaata atcaaagaaa tgaacaacag
      661 ccccctgatt tagaagaagt atttagcagt ttattaaaaa aaatgggggg tggaaaaggg
      721 cctaataata gttcaaatca acctagtgca agcttaggga aatttctacc tgtggtttta
      781 ggtcttgcag cagtagtttg ggttggctca ggtttttaca ctgtgcaaga ggctgaacgt
      841 ggtgtggtta ctcgtttagg taagctgaat gatatcgtgt tgccagggtt gaattggaaa
      901 ccgactttta ttgattcggt tacaagagta aacgtagaac gtgtttcaga gctgaacacc
      961 agtggctcaa tgctgactca agatgaaaat atggtgcagg tagaaatgac ggttcagtat
     1021 cgtgtggaag acccggctaa atatctgttt agtgtgagca accctgatga cagcttaaaa
     1081 caagcaacag acagtgcctt gcgttatgtg attggtcata tgacgatgga tgaaatttta
     1141 accacagggc gtgcaaccgt gcgtgaaaga acgtggtcga cattgcgtga tattatcaaa
     1201 acctatgata tgggtttatt ggtaacagac gtaaacttcc aatatgcacg accgcctgaa
     1261 gaggtgaaag ccgcctttga tgatgcaatc aaagcacaag aagatgagca acgcttaatt
     1321 cgtgaagcgg aagcctatgc aagaggtgaa gagccgattg cacgtggcca ggcacagcgt
     1381 acgattgagc aagctcaagc ttacaaagaa gcagttgtgt taaatgcgaa aggggaagtg
     1441 gagcgtttga gtcaattatt acctgaatat aaagcctctc cagagctgac tcgtgaacgt
     1501 ttatatatcc agacaatgga aaaagtcatg aaaaatacgc cgaaagtggt gatggattca
     1561 agtggtaata atttgaatgt attgcctttc gataagctaa tgaattcttc atcggtaata
     1621 aaagcggagc agcctgcggc ggcacaagta gttacaccac aagttgttca gccgcaagta
     1681 cagccaagaa cacaaattca gccgcaacaa gcagaacaac aagaaccaac tcgtaagggg
     1741 agattttaat catgcgtaga ttattagtac ctatacttgc tgtagttgct tttgttgtgc
     1801 tacaggcaat caccattgta aacgagggcg agcgtggcat tatgttacgt tttaacaaag
     1861 ttcatcgtga tagcgatcaa aaagtggtgg tatatgaacc ggggattcac tttaaagtac
     1921 catttatcga tagtttaaag gtattagatg ctcgtattca aactctagat gggcaagaag
     1981 accgttttgt aacagtcgag aaaaaagatt tattagtgga ctcctatgta aaatggcgaa
     2041 tcagtgattt tggccaattt tatacctcta ctggggggga ttaccaaaaa gcggcggatt
     2101 tattacgccg taaggtaggg gatcgtctgc gttccgagat tggttctcga accattaaag
     2161 atattgtatc cggctctcgt ggtgagttaa tggcaggagc ccaaaaagcg ctgaatgccg
     2221 gtgaagatgg agcagagcgt ttaggaattg aagtggttga tgtgcgagtg aagcaaatca
     2281 acttaccaaa tgaagtttct tcttcaattt accaacgtat gcgtgccgag cgtgatgcgg
     2341 ttgctcgtga acaccgctca caaggtaatg agaaagcaga ggtaattcgt gccgaagtgg
     2401 ataaaaaagt ggtgctgatt ttggcaaatg cgaacaaaac tgcccaagcc ttgcgaggtg
     2461 aaggtgacgc tcaagctgca aaactctatt cagagaaatt tggtaacgag ccggaatttt
     2521 acagttttat tcgtagttta aaagcctatg aagatagctt tgccgaaggt caaaataata
     2581 tgatgttatt aaagccaaac agcgaattct tacgctttat gcaagcaccg acaaaataat
     2641 tgtatctata aaacccgatc ttgtatcggg tttttaattt tcatccctat tctgatcata
     2701 acaagcggta aattttggct gattttttgc aaaaaatttg aaaaaattga ccgcttgcga
     2761 tatgaagtta taatgggttt cgttattcat cctaactaaa agaggcattt ctatgggtaa
     2821 aagtgttgct attcttggcg ctcagtgggg cgatgaggga aaaggcaaaa ttgttgattt
     2881 attaacagat cgtgtgaaat atgtagtacg ttatcaaggt ggacataatg cagggcatac
     2941 cttaatcatt aatggtgaaa aaaccgttct gcgtttaatt ccatcgggta ttttgcgtga
     3001 taacgtgact tgtttaatcg gtaatggtgt agtgctttct ccagaggcat taatgaaaga
     3061 gatgggggag ctagaagaac gaggcattaa tgtgcgtgag cgtttaaaaa tttctgaagc
     3121 ttgcccgtta attctgcctt atcacgttgc gatggatcac gctcgagaag cggcacttgg
     3181 taaaaacaaa attggtacaa caggacgtgg tattggccca gcttatgaag ataaagtcgc
     3241 tcgccgtggt ttacgtgtca gcgatttatt cgacaaagaa cattttgccg caaaattaaa
     3301 agatatttta gattactaca acttccaatt agtcaattat tacaaagttg aagcggtaga
     3361 ttatcaaaaa accttagatg atgtgtttgc gattgcggat gtgattaccg gaatggttgc
     3421 cgatgtgaca actttacttc acgaagcacg tgaaaacggc gacaatattt tatttgaagg
     3481 ggctcaaggc acgatgttag atatcgacca cggtacttac ccgttcgtaa ccagctcgaa
     3541 tacgacagca ggcggtgtgg caaccggatc aggctacggt cctcgtcatt tagattatgt
     3601 attagggatt atcaaagcct actgtacccg tgtaggtagt ggaccgttca ccaccgaact
     3661 atttgacgat gtaggtgctg aaattgctcg caaaggtaat gagttcggag cggtaaccgg
     3721 tcgccctcgc cgttgtggct ggtttgatgc cgtagccgtt cgccgagcag tgcaaatcaa
     3781 ctccatttca ggcttctgta tgactaaact cgatgtttta gacggattca aagagctaaa
     3841 aatctgcgtt gcctacaaaa tgccaaacgg aaaaattgtc gaatatgccc ctcttgcagc
     3901 aaaagactgg gaaggtgttg aaccaattta tgaaacaatg ccgggttggt ctgaaaatac
     3961 tttccgtgtg acaaaacgtg aagatttacc acaagccgca ttagattaca ttaaacgtat
     4021 cgaagagctg gtcggtgtgc cggtagatat tctttctaca ggtccggatc gtgtcgaaac
     4081 gatgattttg cgtgatcctt ttgcagctta ataaaagata aaataaagcc atttagtgag
     4141 atagagctag atggcttttt tgtttgaacc agtaaacttt actttggcac aattttgtca
     4201 caatacagaa aatagttctt tagcgtgagt ttttgaggct gtgtaagtat ttgaatttaa
     4261 aagaatttaa tggcacgccc taaaggattc gaacctttga cccacgcctt agaagggcga
     4321 taggtatttt ataaaaggcg tttattttaa ctaataaaaa caaagactta tagagataac
     4381 ccctctatat tacctaccat tatttcacat ctttttacat taaataacac catttacggt
     4441 acatttatgg aacacgttgg tgatgagcta gtcttcaaaa gttactaaat taaactacaa
     4501 tctgccccgt ctcttttaag tacttataga tacgccctaa cataatctgc gttggcgttt
     4561 tgccaatatc ctcttttgtt agcggtgcat cagtaatctc cttggcggac tgtgcatcaa
     4621 tgtatttgta ctcacttgtg atgattacaa agtttgttac ttctccatcg gtatcatcgc
     4681 ctgtaccgac cacatatt
//
LOCUS       DANLXQ010000174         4482 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.176, whole genome shotgun sequence.
ACCESSION   DANLXQ010000174 DANLXQ010000000
VERSION     DANLXQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.176"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..164)
                     /locus_tag="PXD94_002339"
     CDS             complement(<1..164)
                     /locus_tag="PXD94_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011497995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6287152.1"
                     /translation="MFYSNNPLIKHKSGLLNLAEELGNISQACKVMGMSRDTFYRYQQ
                     AVEQGGVEALL"
     gene            complement(248..787)
                     /locus_tag="PXD94_002340"
     CDS             complement(248..787)
                     /locus_tag="PXD94_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6287153.1"
                     /translation="MLDSLKEVNQKLDKKADKVGGGTKEDALVTLDTLIASGTVKAET
                     AMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGSFEL
                     AYMASWLPQQMAAAKQAGLSSIEGFERLLIANQQARVTAGTSDEAGNNLVNLLGKITA
                     KETNERFKNIEYKSVEDQT"
     gene            886..1137
                     /locus_tag="PXD94_002341"
     CDS             886..1137
                     /locus_tag="PXD94_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6287154.1"
                     /translation="MAPTISDGDQMLVDFSKKEARGDDKIYLVQNGESVWVKRVRKVW
                     DYVELISDNESYRPIRITADEAQNLQIIGQVVHIGHSLV"
     gene            complement(1333..1533)
                     /locus_tag="PXD94_002342"
     CDS             complement(1333..1533)
                     /locus_tag="PXD94_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL6287155.1"
                     /translation="MQYVLDLTGYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENVGY"
     gene            complement(1592..2104)
                     /locus_tag="PXD94_002343"
     CDS             complement(1592..2104)
                     /locus_tag="PXD94_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HDL6287156.1"
                     /translation="MQQLEPQTFASWAEPIDMLYACHSKVKSFCKQLQILPDYLEKNG
                     VNQAVKNDVQQILNYFNISAPLHHEDEEDDFFPELIKVQPQAQAEVDELERQHVHLHK
                     NWDYLSAQLEDLLAGKRENVDRDLIERFVAGYDVHIAIEEPLFELGREHLAQDKLSAM
                     GKVMADRRKL"
     gene            complement(2117..3271)
                     /gene="tgt"
                     /locus_tag="PXD94_002344"
     CDS             complement(2117..3271)
                     /gene="tgt"
                     /locus_tag="PXD94_002344"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL6287157.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRADTTPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVKEQK"
     gene            complement(3330..3470)
                     /locus_tag="PXD94_002345"
     CDS             complement(3330..3470)
                     /locus_tag="PXD94_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287158.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            3564..4136
                     /locus_tag="PXD94_002346"
     CDS             3564..4136
                     /locus_tag="PXD94_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL6287159.1"
                     /translation="MKILRKFAAVCVVSMAMLSANANLLTISEQEINDYLATKLAEKI
                     PLKDKVGIPKLFELDYRLHSLTSQIGRTEEKKVAISGVVDGLLNAKGKKYAASIHLNM
                     DTTPYYDPQKGALFLKDVRLLSWSATPAKYQAELQMFLPVLADGLSSFLNSTPVYTLD
                     ENKTKEAMVKKFGKAIVVENGNIKLETTIF"
     gene            4238..4456
                     /gene="cspD"
                     /locus_tag="PXD94_002347"
     CDS             4238..4456
                     /gene="cspD"
                     /locus_tag="PXD94_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL6287160.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
BASE COUNT         1302 a          971 c          835 g         1374 t
ORIGIN      
        1 agcaatgctt caacaccacc ttgctcaacc gcttgttgat aacgatagaa tgtatctcgg
       61 ctcattccca ttactttgca ggcttgagaa atattaccca gttcttctgc taaatttaat
      121 aaaccgctct tgtgtttaat gagagggttg ttagaataaa acatgagagt ttcctttttg
      181 tttagattga tttttgacac tcatattcta aacgggaaac tctcactttt tagagtgaat
      241 tgtcagatca agtctgatct tctacacttt tatattcaat atttttaaaa cgctcatttg
      301 tttctttagc tgtaatctta ccaagcagat ttaccagatt attacctgcc tcatcgcttg
      361 taccggcagt gacacgagcc tgttggtttg caattaaaag tcgctcaaag ccctcaatac
      421 tactcaaacc ggcttgtttt gcagctgcca tttgttgagg caaccaactt gccatatatg
      481 caagctcaaa actacctgct tgcccggctg caacagccat atccaatgcc cgcccaatat
      541 cctcttcttt gatgccaaat tgttgcatag aggagatcgc aatttttgcc atatcctccg
      601 agcttgcccc tgttgccacc gcccctttct gcaaagtagg taatagactc attgcggttt
      661 cggctttgac tgtaccggag gcaatgagcg tatcaagcgt aactaacgca tcctcttttg
      721 tgccaccccc aaccttatcc gcctttttat caagtttttg gtttacttct ttgagcgagt
      781 ctaaaatatc gtccagcttt tccttaagag ccttattcag acagcctatt aagcctaggc
      841 gtaaaagccc ataattgtgg cgtattttgg gctaatggca catcaatggc cccgactatc
      901 agcgatggtg atcaaatgtt agtggatttc agcaaaaaag aagcccgtgg agacgataag
      961 atttatcttg tgcaaaatgg tgaaagcgtt tgggtaaaac gtgtaagaaa agtgtgggac
     1021 tacgttgaat tgattagcga taatgagtcc taccgcccaa tccgaattac agcagacgaa
     1081 gctcaaaact tacaaatcat cgggcaggta gtccacatcg gacactcttt agtttaaaac
     1141 agatttaaaa tagttttaaa accgatcaaa cctatgcaaa aaatatcgca aaaactgccc
     1201 atttttttct aatcctgctc attttgtttt ttgcatagcc ccaaccaaac aataacggcg
     1261 aacccttgta ttcacggggc tcgccgttat ttttccgccc aaattttttt ctttctctct
     1321 atgcaaaaaa gatcagtacc ccacattttc aaccgcttgt agctcacaaa gccaaccttg
     1381 ctctttggca agcagttcaa aatcggtaag gctactctgc tgattgagta aaatttccaa
     1441 gctatcgccc tttttaagat cgttcatcgc tttttttgcc attaataacg gcaaggggca
     1501 ggcatagccg gttaaatcta aaacatattg catagcgaaa tagggaataa agaaaaaggg
     1561 cgaatattga aattcgccca atcacaaaag attagagttt acggcggtct gccatcactt
     1621 tgcccatagc cgataatttg tcttgagcta aatgctctcg cccaagctca aagagtggct
     1681 cttcaatcgc aatatgcaca tcataacctg ccacaaaacg ctcaattaag tctctatcca
     1741 cattttcacg tttacctgcg agcagatctt caagctgtgc cgacaaatag tcccaatttt
     1801 tatgtaaatg aacgtgctgg cgttccagtt cgtctacctc tgcttgagct tgtggttgca
     1861 ccttaataag ctcagggaag aaatcatctt cttcatcttc gtgatgtaaa ggggcagaaa
     1921 tgttgaagta gttcaaaatc tgctgcacgt cattcttcac tgcttggttt accccgtttt
     1981 tctcaaggta atccggcaaa atttgcaact gtttacagaa actttttact ttgctgtggc
     2041 aagcataaag catatcaatc ggctctgccc agcttgcaaa ggtttgtggt tcaagttgtt
     2101 gcatatacac tccttgttat ttctgttctt ttacttcaga ttgtaaaggt ggcacttctt
     2161 tgccaattcg ggcataaaat tccactacaa aatcatcaaa acgatcttct tcgatggcct
     2221 ggcgaatttc tgccattagg cgttgatagt agcgtaaatt atgaatggtg tttagtcttg
     2281 cccctaaaat ctcgccacat ttgtctaagt gatataaata ggctttggtg tagtttttgc
     2341 aagtgtaaca atcacattca ggatcaagcg gtgtggtgtc tgcacgatat ttcgcattac
     2401 gaatttttac aatgccgttg gtcacaaata aatgtccgtt acgggcattg cgggttggca
     2461 ttacgcagtc aaacatatca ataccacggc gaaccccttc gactaaatct tctggcttgc
     2521 ctacacccat taaatagcgt ggtttatctg ccggaatttg cgggcaaacg tattctaaaa
     2581 tgcggtgcat atcttcttta ggttcaccta ccgccaagcc accgactgca tagccatcaa
     2641 agccgatttc aactaatttt tccaccgaga ctttgcgaag ttcttcataa ataccacctt
     2701 gaataatgcc aaataacgca ttcgggtttt caagttcatc aaagcgatca cgagaacgtt
     2761 tcgcccaacg tagtgacatt tccatcgatt tttttgcata atcaaaggtt gccggataag
     2821 gagtacattc atcaaaaatc attacaatgt ctgatccaag atcgtattgg atctccattg
     2881 atttttccgg cgaaaggaaa attttctcac cgctaatcgg gttttggaaa gttacgcctt
     2941 cttctttaat tttacgcagt ttacctaagc taaaaacttg gaaaccgccg gagtcggtta
     3001 aaatagggcc gtgccattgc ataaaatcgt gcaagtcacc gtgcatcttc atcacttctt
     3061 gccccggtct taaccataaa tggaaagtgt tgcctaataa aatttgcgcc cctgtcgctt
     3121 taacttcttc aggagtcatt ccttttaccg tgccataagt gccaacaggc ataaatgccg
     3181 gtgtttcaac gtgaaattcg cctttcgggc ggttaaaagt taaacgccca cgacgagcgt
     3241 taccacttgt tgtttttaat tcgtacttca ttttaatcca caaataaaca gttcgtgaaa
     3301 gcggtcaaaa accaccaaaa atttaccaat tagaatcgaa agtcggtgcc gacccctaag
     3361 ccgattccaa cgccgttaga gccaccaccg gctccaatac tgccacccac accggttgat
     3421 gagcaagcag ctaacactaa aatgccaacg cataataaga gttttttcat tttttgctgt
     3481 cctaaacgaa aaagagagtc tatgatagac ccaatgtatt ctaacctgtt taatggatta
     3541 aacctaattt taaagggtaa attatgaaaa ttttacgtaa attcgctgct gtatgcgttg
     3601 tctcaatggc tatgctctct gctaacgcta atttgctcac tattagcgag caggaaatca
     3661 acgactattt agccaccaaa ttagctgaaa aaattccttt aaaagataaa gtcggtattc
     3721 ctaaattatt tgaattggat tatcgcctgc attcacttac cagccaaatt ggcagaaccg
     3781 aggaaaaaaa agtagctatt tctggcgttg ttgatggact tttaaacgca aaaggcaaga
     3841 aatatgctgc ctctatccat ttaaatatgg ataccacgcc ttattatgat ccacaaaaag
     3901 gggcattatt tcttaaagat gtcagattgt taagttggtc tgcaacaccg gctaaatatc
     3961 aagcagagct gcaaatgttt ttgccggtgt tggctgatgg attaagtagt tttttaaaca
     4021 gtacgcctgt ttatacttta gatgaaaata aaacaaaaga agcaatggtg aaaaagttcg
     4081 gcaaagccat tgtagtagaa aatggtaata tcaagctaga aaccactatt ttttaaataa
     4141 ttttgtgatc ttcgtctaat ttttacactt tgatagttga gcttttacgc gttttattta
     4201 caatccaaat atcgccattc attatttgga gagttctatg gatatcggta ttgttaaatg
     4261 gtttaacaat gtaaaaggat ttggttttat tacatcggat gtatgtgaag gagacatttt
     4321 tgctcatttt tctgaaattc aagcagaagg ttatcgctct ttaaaagtcg gccaaaaagt
     4381 acagtttgaa ttagttcaag gagagcgagg tgcctcagcc tcaaatatca cgcctataag
     4441 cgaaaaaacg ttataatccg tcgttaataa aattattgtt at
//
LOCUS       DANLXQ010000175         4439 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.177, whole genome shotgun sequence.
ACCESSION   DANLXQ010000175 DANLXQ010000000
VERSION     DANLXQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.177"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..121
                     /locus_tag="PXD94_002348"
     CDS             <1..121
                     /locus_tag="PXD94_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HDL6287161.1"
                     /translation="SNMVSKDKKVLVNNSYKRMLEHESSLEKIHRNRKQNNRK"
     gene            complement(236..1207)
                     /locus_tag="PXD94_002349"
     CDS             complement(236..1207)
                     /locus_tag="PXD94_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISApl1 family transposase"
                     /protein_id="HDL6287162.1"
                     /translation="MSTSYRHLTINEREKIMILLAQGKKQAEIAKALGRSSSTISREL
                     KRHALESYSATNAQNSYLKHRQNSKAQRKLEQPEYFNLVQEKFLTENWSPEQISARLK
                     LEKSELSISYSTIYRGIYSGLFDIGERKASRKLRHKGKTRHTKNHHEKRGKIQISNHL
                     NDRPISAQNRSRFGHWKADTVLGKAGGACLLTLTERKSRFELVKKIPAKKAEAVQKAM
                     IELLDSHILRSITPDRGKEFAQHRLVTEALGVEFYFPEPHQPWTRGTNENTNGLLREY
                     FPKHQDINQWSEVDIQQVVNKLNLRPRKCLGWKTPYEVYFKKTLHLV"
     gene            complement(1388..2152)
                     /locus_tag="PXD94_002350"
     CDS             complement(1388..2152)
                     /locus_tag="PXD94_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6287163.1"
                     /translation="MLIEWRNQIVGDKIKAVTWNNYVRHLKSLYNFGLSQGVLNINQN
                     PFNQLFIRESKRRKKTYSTEQLHKIDHILNNNILLPDFLKPSWFILAIIMTLRCTGIR
                     RGQLVKLKIGDVNLSNRTIYISPEINKNHDYHIVPISDSLYLHIERLITELKIRKQTD
                     NCQLFNLNLFSRCSRRRGQPMSQNQLTHIFRHISDLVGFTSSPHRFRHTVATQLMKKP
                     ENVYVVQKLLGHKDISVTLGYIEHDVEMLRETVNLL"
     gene            complement(2308..4287)
                     /locus_tag="PXD94_002351"
     CDS             complement(2308..4287)
                     /locus_tag="PXD94_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL6287164.1"
                     /translation="MQSIFKKIFGKHTSQSAVKSDTVFSIEQDDDGWLYPQNATQLLN
                     TELRQKYLGLLWQQVSMNRQMFETFYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKARQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGEPPEPYKFKYIKGRDYHLHLVIGSFFANQIIPKSAFDWLAQYPEVISSL
                     MYAMSGHNDKAGLLSEIVQKADQYSVTLALGGDVNKLVQRPVNSLSKQLVLALRYLLE
                     QKIKLNTPKGPADGWFTEEGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQSLG
                     IVEESPSGGAIWSCRIQADSGWTPANVFTLLRIKPEVAWENINDRPNYFSGKVKIVEQ
                     IMEDSNMENHPPLVDESTKLVTLENVQPETIITSKEVEISQVEASSDNAPFSEIIIND
                     ENDLTSFVLDMFAPTESSEPSLADISIGKGQEIATMPSKQEVQDKPTTKKLNVTQSAV
                     ETPETLTLSELQSQNKENSITGDNFVNWLKSGIATNKFAINKPTAKLHIVEDHLFLVT
                     PVIFEMYLQESGLPYDKEMINSLQYDFQGLGIHLPKNTTINGKPDTINFWKCDVIGPR
                     KTSSLTGYLIKNTHLFFGDKTLLNNHCLTLQKEET"
BASE COUNT         1276 a          833 c          844 g         1486 t
ORIGIN      
        1 ttcgaacatg gtaagcaagg ataaaaaagt tttagttaac aattcgtata aaagaatgtt
       61 agaacatgag tctagtttag aaaagattca tagaaataga aagcaaaata acagaaaata
      121 gataaacggc agataaggaa aagctaaatg ttaaaattaa agctagaaaa cataaacaaa
      181 actttcgatg ggaaaaactt tgttgttaaa aattttaatt tggaagcttg aattgtcaaa
      241 ccaagtgcaa cgtttttttg aagtaaactt cataaggtgt tttccaaccc aaacatttac
      301 gcggtcgtaa attcagttta ttgaccacct gttgaatatc aacttcgctc cactgattga
      361 tgtcttggtg cttcggaaag tattcacgaa gtaacccatt tgtattttca ttcgttcccc
      421 gtgtccacgg ttgatgcggc tcggggaagt aaaattctac acccagtgct tctgttacca
      481 aacgatgttg ggcaaattct ttaccacggt ctggcgtaat tgaccgcaat atatgtgaat
      541 ccagcaattc aatcatggct ttttggactg cttcggcttt tttggcagga attttcttca
      601 ccaactcaaa acgacttttg cgttccgtca gcgtcagcaa acaagctcca cccgctttac
      661 ccagtacggt atcggctttc caatgtccaa agcgactgcg attttgcgcc gaaatgggac
      721 ggtcgttcaa atggttggat atctgaattt tgccacgttt ttcatgatga ttttttgtat
      781 gccgtgtttt gcctttgtgg cgcagtttgc gactggcttt gcgttcgcct atatcaaaca
      841 accctgaata aataccacga taaatggttg aataactaat ggataattca gatttttcca
      901 attttaatcg tgcgctgatt tgttcgggcg accagttttc tgtcagaaac ttttcttgca
      961 ccaaattgaa atattcaggc tgctctaatt tgcgctgtgc tttgctattt tgacgatgct
     1021 tcaaatagct gttttgtgcg ttcgttgcac tgtagctttc tagagcgtgt cgtttcagct
     1081 cgcgagaaat ggtgctggag ctacgtccca gtgctttggc aatttctgct tgttttttgc
     1141 cctgtgcgag taaaatcatt atcttttctc gctcgtttat tgtaagatgt cggtaggaag
     1201 tactcatcat ttggaagtgg tttttgtgtg gaaacaaaat tatattctag ttgatgagta
     1261 cttctttttt tgttgcactt ggattgtaaa ttcagcgtat aaaatcagtt catttagaag
     1321 ataaaacaga aattatttat ttatagaaaa aaagtgggag aaattatatt ctcccatttt
     1381 tcttttatca gagtaaattc actgtctctc tcaacatttc cacatcgtgc tcaatataac
     1441 ctagtgttac gctgatatcc ttatgcccta gaagtttctg aaccacataa acattttcgg
     1501 gcttcttcat taattgagtt gcaaccgtat gtcgaaatct atgtggtgat gaagtaaaac
     1561 cgaccaagtc agaaatatgc cgaaatatat gcgtaagctg attttggctc attggctgtc
     1621 ctcttcttcg agagcatcga gaaaataaat tgagattgaa caattggcaa ttatctgttt
     1681 gttttctgat ttttaactct gtaataagcc tttctatatg aagataaagt gaatctgaaa
     1741 tcggtacgat atggtaatca tgatttttat tgatttctgg cgaaatataa atcgtcctgt
     1801 tggaaagatt tacatcacca attttcagtt tcactaattg tcctcgccga atgcctgtac
     1861 atctcaatgt cataataatc gcaagaataa accaacttgg ttttagaaaa tcaggtaata
     1921 gaatgttatt attgagtatg tggtctattt tatgtaattg ttcggttgaa taagttttct
     1981 ttcttctttt tgattcacga ataaataact gattaaatgg attttgattt atattgagta
     2041 ccccttgcga tagaccaaag ttatataacg attttaaatg tctgacataa ttattccatg
     2101 ttacagcttt gattttatcg ccaacaattt gattacgcca ttcaattagc atatcagtgg
     2161 taatctcatt tgcatttaac tttgggtaac gtcttttgat aattcggatt acattattat
     2221 agctccgctt agtatcaggg cgaagatagt aacgatagaa aaaaaaatcg gtctagtagc
     2281 gaatcaaaag agatagatgt cgtcatttta agtttcctct ttctgtaaag ttaaacagtg
     2341 attatttaat agtgtcttat ccccaaagaa gagatgtgta ttttttatca aatatccagt
     2401 taaagaggaa gtctttctcg gaccaattac atcacatttc caaaaattga tggtatctgg
     2461 cttaccgttt attgtcgtat tcttaggtaa atggattcct agcccttgga aatcatactg
     2521 aagactattt atcatttcct tatcataagg cagaccactc tcctgcaaat acatttcaaa
     2581 aataacgggg gtaactaaaa acaggtggtc ttcaacaata tggagcttgg cagtaggctt
     2641 gttaattgca aatttgttgg tcgcaatacc tgatttgagc caatttacaa agttatctcc
     2701 tgtaattgag ttctccttat tctgtgactg tagctctgat agtgttaatg tttcgggcgt
     2761 ttctactgca ctttgtgtta cgttgagctt tttagtggta ggtttatcct gtacttcttg
     2821 tttggatggc atcgttgcaa tttcctgtcc cttaccaatt gaaatatcag caaggcttgg
     2881 ctcagagctt tccgtaggtg caaacatatc cagcacaaaa gaggttaaat cattttcatc
     2941 atttatgatg atttctgaga acggtgcatt atctgagctt gcctctacct gagatatttc
     3001 aacttcctta gaggtaataa tagtctctgg ttgaacattc tctaatgtaa caagtttagt
     3061 gctttcatca actaatggag gatgattttc catattgcta tcttccatta tctgctcgac
     3121 tattttaact ttcccggaaa aataatttgg gcgatcatta atattctccc aagccacttc
     3181 aggtttaatt ctcaataatg taaagacgtt tgccggtgtc caacctgaat cagcttgaat
     3241 acggcaactc caaatcgccc caccagaagg agattcttct acgataccga gagattgcat
     3301 ttcatcaaat aactttggat tatccttagg cacggaaatc ccctgcccta gtaagtaagt
     3361 tcgtatatta tcagcggcag ttttgctaac aagccacaat ccttcctcgg taaaccaacc
     3421 gtctgcaggg cctttcggag tatttaactt gattttctgt tccagtaaat aacgtagtgc
     3481 caagacaagc tgctttgaca gagaattgac aggacgttga accagtttat ttacatcgcc
     3541 accgagtgcg agggtaacac tatactgatc agctttttgc acgatttcag ataaaagccc
     3601 agctttatca ttatgtccgg acattgcata catcagagaa gaaatgactt ctggatactg
     3661 tgccagccaa tcaaatgcag atttgggaat aatctgattt gcaaagaagc tgcctatcac
     3721 aagatgaaga tggtagtctc gacctttgat gtatttgaat ttatacggtt caggaggttc
     3781 tccattccaa gcaaaccaac gtgagccatc tttgagtgtg atttcaatat ctactacaac
     3841 tttgccaata tcgtgaacta atgcaagata aattgctgcg gcagtccaag cgtctcgctg
     3901 ctttgactgt tcttcgggag ccacattcgg tggcaaaata taactctggc gagctttagc
     3961 tgaaaaagca ataacttcca aaccgtgatc gagcattcca ccaatatgag aatgatgatg
     4021 tgattctgaa gctggcaata gttgaaccat ttcagcgtaa cgttctattg gttgctgata
     4081 aaatgtctcg aacatttgac ggttcattga tacctgttgc caaagtagtc caagatactt
     4141 ttgacgtaat tccgtattca gtaattgagt agcattttgg ggatatagcc aaccatcgtc
     4201 atcttgctca atagagaaaa cagtgtcaga tttgaccgca ctttgagatg tgtgtttacc
     4261 aaaaattttc ttgaaaattg attgcattga aagagaccga aaagtatcaa ttttgtgtta
     4321 aatgaaagcc gtttagccct ttgccctttt gggcaagacc tttccccttg gaaaggttac
     4381 ctttcaccct ttaggggtta tcctttgccc ttaaggactc ctttatcctt ttgccattt
//
LOCUS       DANLXQ010000176         4408 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.178, whole genome shotgun sequence.
ACCESSION   DANLXQ010000176 DANLXQ010000000
VERSION     DANLXQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.178"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(219..986)
                     /locus_tag="PXD94_002352"
     CDS             complement(219..986)
                     /locus_tag="PXD94_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL6287165.1"
                     /translation="MSKIIKIGKVANNSDAIDAISTQPRHSVIVGTIKRLQTDRIDLL
                     YQHRVDPNVPIEEVAGAIKDLMKQGKVLNWGLSEAGEKTIRKAHAIQPLTAVQSEYSL
                     FFRGREKDVILVCEELGIGFVPWSPLAMGALAGYVDENSRFEADPSKDLRGIIPRFTP
                     EAMQQNVKLIQLVQKWAKRKLCTPAQLSLAWLMAQKAFIAPIPGTSKARHLLDNLGAD
                     DVKFTEEELAQFRQELEAIEIVGLRLPEAILNFSEAK"
     gene            985..1305
                     /locus_tag="PXD94_002353"
     CDS             985..1305
                     /locus_tag="PXD94_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287166.1"
                     /translation="MELKVIHKNLVDSAKIKLHAVYLVIFLFVSHFAMIFGMADPISA
                     GYQPTTPAMTHSMQMDAKQITEMHNQMMTGEITPEEMMKMHQEMNPNMNMPNTQGGHS
                     EHHK"
     gene            1487..1942
                     /locus_tag="PXD94_002354"
     CDS             1487..1942
                     /locus_tag="PXD94_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HDL6287167.1"
                     /translation="MKLHKFTRSLLLLSLGITAFAQAQTLSMEVWKSPTCGCCNEWIS
                     YMQKNGFEVKVNETGNYDAHKRFNIKYEHASCHTAVIDGYVIEGHVPAEDIKRLLAEK
                     PDAIGLSAPGMPIGSPGMDGEAYGGRKDPYDVVLIKKNGESEVFKSYNQ"
     gene            1956..3503
                     /locus_tag="PXD94_002355"
     CDS             1956..3503
                     /locus_tag="PXD94_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HDL6287168.1"
                     /translation="MKRRDFLRYGIGISLASSSLYSLANMMNHAQHGNMAMMHSVPTG
                     LMPTEAMPSGLSLNGILPKLANQSTQAGLFKATLKAEPIKIRLADNKETEFWAYNGQL
                     PGPQIEVFEGDTVEIEFINHLPQPSTVHWHGLDVPNEADGNPMDMVEPQGKKVYRFTL
                     PQGSAGTYWYHPHPHDHVSEQVYKGLAGTFVVKAKNDPLAHLPEQHWVISDLRLNADG
                     TIPANTMLDWINGREGEFVLINGQYQPQIQVKTNERIRLWNATSARYFRLNIPGVKWI
                     VVGTEGGLLEKPRSPVDELLLTPAERVEVIMVGETQGTVNLQSGYYDRRKMMVQEQPK
                     DLTLATIQVKSEPVQLPESLRSLPNWDEPKQVRQIRFSEKMMNHTNMPMMNHGTHHAT
                     TTLTDNPIPPMMNGMFLINGQTFDMNRIDFVTKLNEVEQWEIFNESHMDHPFHLHGTQ
                     FEVIQHTLNGKTFKPEGRALKDVVNLRPYEKVIIRFKQGHTGLKMYHCHILEHENLGM
                     MGMFKVE"
     gene            complement(3593..3748)
                     /locus_tag="PXD94_002356"
     CDS             complement(3593..3748)
                     /locus_tag="PXD94_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL6287169.1"
                     /translation="MGLNRAEMMYREGAYVIDPVFPATLGYAGAGVVVAVGDDAGEFQ
                     IGDKVSV"
     gene            3837..4016
                     /locus_tag="PXD94_002357"
     CDS             3837..4016
                     /locus_tag="PXD94_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287170.1"
                     /translation="MFLVNQLLAITIATSAVLTNAQLAILAVAFWIAILPCARLATAV
                     GVWVFDDVVHNNSPN"
     gene            4065..>4408
                     /locus_tag="PXD94_002358"
     CDS             4065..>4408
                     /locus_tag="PXD94_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287171.1"
                     /translation="MPFKTVTFAQATTNDGQSLLAVNDLFIGPKSHTSAQYILQWNGA
                     EEVQSSSGIIVSTGLGSTGWFQSILAGAMAITGEASHPLLQGFSWSDRKLQFSVREPF
                     PSRTTGVALTFGT"
BASE COUNT         1353 a          914 c          888 g         1253 t
ORIGIN      
        1 tgattatgcc atttttacac ttattgacta tagcgttttg cctaatcatc tgtctgattc
       61 cagttttacc cttttcccaa tatctttcaa tttcgataaa tcttccacta atcttgctac
      121 atattcaaga aaaagtgcga tcgtttttca aaggatttta tgaaaacgac cgctgtaaga
      181 aagaattttt atttacgact ttttcaggta tctgaaaact atttcgcttc tgaaaaattc
      241 aaaattgctt caggcaaacg caagccgaca atttcaatgg cttctaattc ttggcggaat
      301 tgcgccaatt cttcttcggt aaatttcaca tcgtctgcgc ctaaattatc caacaaatga
      361 cgggctttgc tcgtcccagg aattggcgcg ataaacgctt tctgtgccat tagccaggcc
      421 aaagaaagct gtgcaggcgt acaaagtttg cgttttgccc atttttgcac aagctggatt
      481 aacttcacat tttgttgcat cgcttcgggt gtaaagcgtg ggataatacc acgcaaatct
      541 ttgcttggat cagcctcaaa acggctgttc tcgtccacat aaccagccaa tgctcccatt
      601 gccagcggcg accaaggcac aaacccaatc cctaattctt cacaaacgag aatgacatct
      661 ttttcacgac cacggaaaaa caacgagtat tcgctttgta cggcggttaa aggctgaata
      721 gcgtgtgcct tgcgaatggt tttctcgcct gcttccgaca agccccaatt caaaactttg
      781 ccttgtttca ttaaatcttt aatagcgcca gccacctctt caatcggcac attgggatca
      841 acacggtgtt gatagagtaa gtcaatacgg tcggtttgta agcgttttat cgtaccgact
      901 attactgaat ggcgtggttg ggtagatatc gcatcaattg catcactatt attagctacc
      961 tttcctattt ttattatttt actcatggaa ttaaaagtta ttcataaaaa tctcgtggat
     1021 agtgcaaaaa ttaaattaca tgcggtttat cttgtgattt tcttatttgt gagtcacttt
     1081 gctatgatct ttggcatggc agatcctatt tcagcaggct atcaacccac tacaccagca
     1141 atgactcatt caatgcaaat ggatgctaaa caaataacag aaatgcacaa ccaaatgatg
     1201 acgggcgaaa taacgcctga agaaatgatg aagatgcatc aagaaatgaa ccctaatatg
     1261 aatatgccga atactcaagg tggtcattca gaacatcata aataataatt ctgatcacat
     1321 tttaaaaaat aatagtggat ggtacgttgg catatctcta ctacaagcag taggattttg
     1381 agaaaaattt tcaaatgata gaattttttc agattacttg accttaaccc taggttaagc
     1441 tttaaaatga tgtccattca atctcaataa aaaataagga catcttatga aattacataa
     1501 attcacccgc tcattgttgc ttttaagttt aggcataacc gcttttgctc aagcacaaac
     1561 actttctatg gaagtgtgga aaagcccgac ttgtggctgt tgtaatgaat ggatcagcta
     1621 tatgcagaaa aacggttttg aggttaaagt caacgaaaca ggcaattatg atgctcataa
     1681 gcgctttaac attaaatatg aacatgcttc ttgccacaca gccgtgattg atggctatgt
     1741 gattgaagga catgtacctg ctgaggatat caaacgttta ctcgcagaaa aacccgatgc
     1801 aataggcttg tctgcaccgg gaatgccaat tggttcacca ggtatggacg gtgaagcata
     1861 cggcggacgc aaagatcctt atgatgttgt tttgattaag aaaaacggtg aaagtgaagt
     1921 gtttaaatcc tataaccaat aggggggggg aaagaatgaa acgtcgagat tttttacgtt
     1981 atggaattgg aattagctta gccagtagct cgctttatag cctcgcgaat atgatgaacc
     2041 acgcacaaca tggcaatatg gcgatgatgc attcagtacc tactggccta atgccaactg
     2101 aggcaatgcc ttcaggttta tcgcttaatg ggatattacc aaaacttgct aatcaatcga
     2161 ctcaagctgg actgttcaaa gccactttaa aagcagagcc aattaaaatt cgcctcgcag
     2221 acaataaaga aacggaattt tgggcttata acggacaact accagggcca caaattgaag
     2281 tatttgaagg agataccgta gaaattgaat ttatcaatca cctaccacaa ccctcaacag
     2341 tacattggca cggcttagat gtaccaaatg aggcggatgg taacccgatg gatatggttg
     2401 aaccgcaagg gaaaaaagtc tatcgcttta cgctacctca aggcagtgct gggacatatt
     2461 ggtaccaccc tcatccacat gaccatgttt ccgaacaagt ctataaaggc ttagcaggca
     2521 ctttcgttgt taaagcgaaa aatgatccgc ttgcacacct tcctgaacaa cattgggtga
     2581 tttccgatct gcgtttaaat gccgatggca ccattccagc aaacacgatg ttagattgga
     2641 taaacggtcg tgagggtgaa tttgtgttaa tcaacggtca atatcagccc cagattcaag
     2701 tgaaaacgaa tgaacgaatc cgtctttgga atgccactag cgctcgttat tttcgtttaa
     2761 acattccagg ggtgaaatgg attgtggtgg gtaccgaagg tggtttactt gaaaaacctc
     2821 gctcgcctgt tgatgaactg ctacttacac cagccgaacg cgttgaagtg attatggtgg
     2881 gtgaaacgca aggaacagtc aatttacaaa gcggttatta tgatcgccgt aagatgatgg
     2941 ttcaagaaca gcctaaagat ctcactttag ccacaattca ggtgaaatca gaacctgttc
     3001 agttacctga aagcttaagg tccttgccta actgggatga gccaaagcag gttcgccaaa
     3061 ttcgctttag tgaaaaaatg atgaaccata cgaatatgcc aatgatgaat cacggtacac
     3121 atcacgctac tacaacacta acggacaacc ctattccacc aatgatgaat ggtatgttct
     3181 taatcaacgg tcaaaccttt gatatgaacc gtattgattt tgttaccaaa ttgaatgaag
     3241 tggaacagtg ggaaatcttc aatgaaagcc atatggatca tccattccat ttacacggca
     3301 ctcaatttga agtaattcaa catacgttaa acggtaaaac ctttaagcca gaaggcagag
     3361 cgttaaaaga tgtggttaat ttacgccctt atgaaaaagt gattattcgc tttaagcaag
     3421 gacatactgg gttgaaaatg taccattgcc atattttaga acatgaaaat ctcggtatga
     3481 tggggatgtt taaagtggaa tagtggtatc taactataaa aatagaggga tttcgtcata
     3541 aaaaatctgc accttaatca gttggttttt agtccaactt ttggggtgca gatcagacgc
     3601 tcactttgtc gccgatttga aattcgccag cgtcatcgcc cactgccacg accacgcctg
     3661 cccctgcata acccaaagtc gcagggaaca cagggtcaat cacatacgcc ccttcccgat
     3721 acatcatctc ggcacggttt agtcccaatg cacggcacgg tttttaaaca ttttcattat
     3781 agttttcctt atcaaaatgg gatttggcaa acattgggca aacctaagcc cgcccaatgt
     3841 ttttagttaa tcaattactt gcaatcacaa tcgccacaag tgcagttttg acaaatgcac
     3901 agctcgccat tttggcagtt gcattttgga ttgcaatttt gccctgtgca agattggcaa
     3961 ccgcagtggg cgtttgggtt tttgatgatg tcgttcataa caattctcca aattaaaggt
     4021 tgattatcaa aacaaaaacc gtttttaaac ccattaataa aaaaatgcca tttaaaacgg
     4081 tcacttttgc acaagcaaca accaatgatg gtcaatcctt attagcggtt aatgacttat
     4141 ttattggccc taaaagccac acttccgcac agtatatttt gcaatggaat ggcgctgaag
     4201 aagtgcaatc ttcatcaggc attattgtat caacaggatt gggatcaacg gggtggtttc
     4261 aatctattct tgctggtgcg atggcaatta caggagaagc ttcgcaccct ctattacaag
     4321 gctttagctg gagtgatcga aagctacaat ttagtgtaag agagccattt ccaagtagaa
     4381 caacaggtgt tgcactgact tttggcac
//
LOCUS       DANLXQ010000177         4390 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.179, whole genome shotgun sequence.
ACCESSION   DANLXQ010000177 DANLXQ010000000
VERSION     DANLXQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.179"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1081)
                     /locus_tag="PXD94_002359"
     CDS             complement(<1..1081)
                     /locus_tag="PXD94_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL6287172.1"
                     /translation="MGIMSKSISIFNHKGGIAKTTTAFNVGWSLANQGYQVLLVDLDS
                     QCNLTGLVLGYDQCKEDSDLALFYNNRHHLTMEGIVEALINGQSPDNYLSGNVGQITQ
                     TLNPNLFLLPGHLNVSELDSQISVSLKIAAGVPATRNIPGNLPTLIKKVAEQNNIDYI
                     LYDLSPNVGGLNEVILMSSDYFIVPTSPDFFCWQAVGSLAKNIRKWHNELKAFKELNG
                     FNQSSYAIKNKPQFLGTIQQRYRPRKGQPAKSFEKWIERIRNEVITNFVPVLKTIDCA
                     IDDTAIYAALKSTNSDLEPFDLAHIADFNSLIAISQELRVPVFSLTKEQIKNSGQFGH
                     ALNTMDESKENFDQEFQSLAERIIQL"
     gene            complement(1146..1577)
                     /locus_tag="PXD94_002360"
     CDS             complement(1146..1577)
                     /locus_tag="PXD94_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287173.1"
                     /translation="MKKMAIVALSAFFSMNAFANNNVVYSCTTTDNQTLKVTKEGGNY
                     VYSHGNTTFKNPVKEALKNPASEIAGGSQFTTISLELRNAGKSYIVGHIEADPKSPFE
                     ASVQIQDIKTGNDITSFECRSDKPIRHNFDRKLMRKSGFAA"
     gene            complement(1581..3683)
                     /locus_tag="PXD94_002361"
     CDS             complement(1581..3683)
                     /locus_tag="PXD94_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL6287174.1"
                     /translation="MKKAKSLKFLTACIMLTTMSGYANSNTVNVGNSISNFIENTTGY
                     RTGNLGVSGNNALTEYYPVLSDYMHYSIGGGSAISAPASRGRPNQYGIGIGWKSNLTC
                     GNFDIKLSIKNQLNGITEGFKDLYGNIIESATGAVASLPAMIIQRANPQLYDILSNGL
                     YQGKLDFNNFKTSCEAMSNQLADLAMDSKLAKMAGLHRAAEIAETEPDAMAAKKKMDK
                     KDAEKGIPWVGGEQRGGKNQKPMAIVADIVGAGYNLALGRGPLEKGDIPIASCDGMLC
                     TEWKNPSEAAAYASEVIGETFLDACETCEQSSSGAKAGKGIAHFIEQTTIEKATKLEA
                     ALNHLDSLTPEQLNSLSTSTVSVTKGVLEAIAASPDAALLGARLSQELAISKEMEKLL
                     LIRRAMLAGMREPNVIKSSLVEEAERNLKLLDREIEQVKLEMELQRSINGNTALAAIT
                     QQMNEQALAIDDNAMGDGNRRFANLSNQAGANNLNTSGIANSAQNAVGDRYIAIPKSD
                     GSGMSDFYGAYKASPTNIVSNVNVGSYTQIAQMNGNANEQATALIKQFEGYLSHGKWD
                     VNANRAGYGSDTYTTADGMVHRVQKGTYVSREDAERDLARRMPQFMNVARGNVGADVW
                     AKLTPQAQAALTSVSYNYGSLSKLESVQKAAAVSAQSGDMTALANSIRNLQGHNNGVN
                     ARRRNQEADYIINAPKGN"
     gene            complement(3710..3988)
                     /locus_tag="PXD94_002362"
     CDS             complement(3710..3988)
                     /locus_tag="PXD94_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL6287175.1"
                     /translation="MQAKTFYYLPLLLLSAMASASPANKPSDVTVKVFTSESYPKVFP
                     HSGDWATHSDSFSNTENYSWALWRPYSCCKKQGQVFLGHVDWKSTHTH"
BASE COUNT         1223 a          872 c          834 g         1461 t
ORIGIN      
        1 tcagttgtat aattcgttct gctaatgatt gaaattcttg gtcaaaattt tctttacttt
       61 catccattgt attcaaagca tgtccaaatt gacctgagtt tttaatttgc tcttttgtta
      121 aggaaaatac gggaactcgt agctcttggc taatagcaat caacgaatta aagtcggcta
      181 tgtgagccaa atcaaatggc tctaagtcag aattcgttga tttaagagca gcataaatag
      241 ctgtatcatc aatagcacaa tcaatagttt ttaataccgg cacaaaattc gtaatgactt
      301 catttctaat tcgctcaatc catttttcaa atgatttggc cggttgccct tttcttggtc
      361 gataacgttg ctgaatagta cctaaaaatt gtggcttatt tttaattgca taagatgatt
      421 ggttaaaacc attaagttct ttaaatgctt ttaactcatt atgccatttg cgaatatttt
      481 tagcgagcga accaactgct tgccagcaga aaaaatcagg tgatgtaggt acaataaagt
      541 aatcactaga cattaggata acttcattta atccacccac atttggacta agatcataga
      601 gtatgtaatc aatattgttt tgttcagcta ctttcttaat taaagtcgga agattgccgg
      661 gtatatttct tgttgctgga acaccggcag caatttttaa tgatacacta atttgcgaat
      721 caagctcgga aacattaagg tgaccaggta acaagaacag atttggattc agggtttgag
      781 tgatttggcc aacatttcca ctaaggtagt tatcagggct ttgtccgttt atcagtgctt
      841 caacaatacc ttccatagtc aaatgatgtc tattattgta aaaaagcgct aaatcactat
      901 cttctttgca ttgatcgtag ccaagtacca gaccggttag gttacattgt gaatccaaat
      961 cgactaacaa tacttgatag ccttgatttg ctaaactcca accaacatta aatgcagttg
     1021 tagttttagc aatacctcct ttatgattaa aaatactgat agacttactc ataataccca
     1081 taattctatg aaataattaa cccaccgaac tcatttgaga atggtgggtt aatgctttgc
     1141 tttagctagg ctgcaaatcc agattttctc atcagtttgc gatcaaagtt gtggcgaata
     1201 ggcttatcgg aacgacattc aaatgatgta atatcatttc ctgttttaat atcctgaatc
     1261 tgaacacttg cttcaaaagg tgatttaggg tctgcttcta tatgacctac aatataagat
     1321 tttcctgcat ttcttaattc gagcgagatt gttgtgaatt gtgaaccacc tgcaatctcc
     1381 gatgccggat ttttgagagc ttcttttaca ggatttttga atgtggtatt accgtgagag
     1441 tacacatagt ttcccccctc tttggtaact tttaacgttt ggttatctgt agttgtgcaa
     1501 gaatacacta cattgttatt tgcaaaagca ttcattgaga aaaatgctga taaagctaca
     1561 attgccattt ttttcatagg ttagtttcct tttggtgcgt tgataatgta atcggcttcc
     1621 tggttacgtc ttcgagcatt aacaccatta ttatgccctt gtaaatttcg gattgagtta
     1681 gctaaagccg tcatatcacc tgattgtgcg gagacggcag ccgctttttg aacactttct
     1741 agtttagata atgacccata gttgtatgat accgacgtta atgctgcttg tgcttgagga
     1801 gtcaatttag cccaaacgtc ggcaccaaca ttaccccttg caacattcat aaattgaggc
     1861 attcttcgtg ctaaatcacg ttcagcatct tcccggctta cataagtgcc tttttgaacc
     1921 cgatgaacca taccatctgc agtagtatag gtgtcagagc catatccggc acggttagca
     1981 tttacatccc atttcccgtg gcttaaatat ccttcaaact gtttgataag agccgttgct
     2041 tgttcattcg cattgccgtt catttgggca atttgggtat agctccctac attcacgtta
     2101 ctgacaatgt ttgttgggga ggctttatat gctccataaa aatcactcat gcctgagccg
     2161 tctgatttgg ggatagcaat atatctatcg ccaacggcat tttgtgcaga attagcaatg
     2221 ccactggtgt tgagattatt tgctccggct tgattgctta aattggcaaa gcgacggttt
     2281 ccatcaccca tcgcattatc atcaatagca agagcttgtt cgttcatctg ttgagtaatt
     2341 gcagctaatg ctgtattacc gttgattgaa cgttgcagtt ccatttcgag tttaacttgc
     2401 tcaatttctc tgtcaagtaa tttgaggttt ctttctgctt cttctactaa ggaagatttt
     2461 ataacatttg gctcacgcat accggctaac attgctcggc gaatcagtag caatttttcc
     2521 atttctttag aaatagctaa ttcttgcgat aaccttgcac ccagcaaggc tgcatccggc
     2581 gaagcagcaa tggcttctaa tacgcctttt gttacactga ccgttgatgt actcagtgaa
     2641 ttgagttgtt ccggcgttaa actatctaag tgattcagtg ccgcttctag ctttgtggct
     2701 ttttcaatag ttgtttgttc aataaaatgg gcgattcctt tcccggcttt agctccactt
     2761 gaactttgtt cacaagtttc acaggcatca aggaatgttt caccaatcac ttcacttgca
     2821 taggcagcag cctcgcttgg atttttccat tccgtacaaa gcataccatc acagcttgca
     2881 ataggtatat ctcctttttc taatggtcct cgtcctaaag cgaggttata tccggctcca
     2941 acaatgtcag caacgatagc cattggtttt tgatttttac caccacgttg ttcgccacct
     3001 acccaaggga tgcctttttc agcatctttt ttatccattt tcttcttggc tgccattgca
     3061 tcaggctctg tttcagcaat ttcagcggct cggtgtaatc cggccatctt tgctaattta
     3121 ctgtccattg ctaaatcagc aagttggtta gacatggctt cgcaactggt tttgaagttg
     3181 ttgaagtcaa gttttccttg atatagacca ttagaaagaa tgtcatacag ttgaggattt
     3241 gctcgttgga taatcatcgc cgggaggctg gcaactgcac cggttgctga ctcaataata
     3301 ttgccataaa gatctttaaa accttcagta ataccattaa gctggttttt aatggaaagt
     3361 ttaatatcaa agttgccaca agttaaattc gatttccagc caatgccaat cccatattga
     3421 tttggtctgc cacgggaagc tggtgcggaa atggcactac ctcctccaat ggagtagtgc
     3481 atataatcag aaaggacagg gtagtattcc gtgagagcgt tattaccaga aacgcctaga
     3541 ttaccagtcc tatagcctgt tgtattttca ataaaattac taatgctatt gccaacatta
     3601 acggtattac tatttgcgta tccgctcatg gttgtgagca ttatacaagc ggttagaaat
     3661 ttgagacttt ttgctttttt catttattta tcctaatagt gctgataatt taatgagtat
     3721 gtgtactttt ccagtccaca tgacctaaaa aaacttgccc ttgtttttta cagcaagaat
     3781 agggcctcca taatgcccag ctgtaattct cagtattgga gaaactatct gaatgggtag
     3841 cccaatcacc actgtgcgga aagactttag ggtaggattc ggaagtaaat accttaactg
     3901 ttacatctga gggtttatta gcaggactag cacttgccat agctgaaagc agtagtagtg
     3961 gtaaatagta aaatgtctta gcttgcataa atgctccaca aatagagtaa ttcacgttta
     4021 ccttaaaaga tgatttggtt agaagaaatc ggaaattgtt ttttggtttt ggagagtttt
     4081 tgttagtata taagttaatg taacaaggga gagtggatgt atgagcaata gaatacctaa
     4141 agcctgttat gcttacccaa aatcgccgaa caacccgttt aatagattgt cggttatcaa
     4201 tctataccag ccgaaatgtc tcccagcatt ctgaaaggga actctgtaaa attacaggaa
     4261 tgccacctaa acaataaatg gataatgcca gccactttat ccgttgtatt ttgtcacatg
     4321 cgtgattgca atgtcataat attacccgac ttggggatat tatgacattg caatcattgc
     4381 tcacttttct
//
LOCUS       DANLXQ010000178         4348 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.181, whole genome shotgun sequence.
ACCESSION   DANLXQ010000178 DANLXQ010000000
VERSION     DANLXQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.181"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..174
                     /locus_tag="PXD94_002363"
     CDS             <1..174
                     /locus_tag="PXD94_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6287176.1"
                     /translation="PRRVFERSAEKKKIHNLISRAHYDMATNEDSASEHLELFGEDIA
                     EDSFDYPKPEKLI"
     gene            181..252
                     /locus_tag="PXD94_002364"
                     /pseudo
     CDS             181..252
                     /locus_tag="PXD94_002364"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            262..780
                     /locus_tag="PXD94_002365"
     CDS             262..780
                     /locus_tag="PXD94_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL6287177.1"
                     /translation="MNRRYIGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGS
                     FRFCELGEEVFDAFGSLNPNIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYY
                     LLYNGILGDKRPQSGNVLTRKLMAELPYFAEFIQQGMDIVVYGEACRLGEVQLAEKKI
                     TFKQIPYDVMSR"
     gene            796..1785
                     /locus_tag="PXD94_002366"
     CDS             796..1785
                     /locus_tag="PXD94_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL6287178.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSNLRDVPYVCVRIPTGGGKTLLASHSIARIAKQYLDCDFPVTLWPVPSRTIKTQTTE
                     ALKNPQHPYRAVLDKTFNREVMVIESEDFDLLRPQDFGNKAIVIVSTIQNFRVENQDG
                     RKIYAFNEKLTAHFERLLPHIQANLEKISTTDLQENGLTSKQTGKIKCSFANLLRAYQ
                     PLVIVDEAHNARTSLSFDVLSQLSPSAILEFTATPNTDRKAGSNVLYHVSASHLKAEE
                     MIKLPIVLTEHHNWQQAIDGAVINRGALAQKAQYESEYVRPIYCFKLSRKTAK"
     gene            1752..2981
                     /locus_tag="PXD94_002367"
     CDS             1752..2981
                     /locus_tag="PXD94_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287179.1"
                     /translation="MFQAEPKNGKVTVEVLKQYLIDELKIDEEEIAIVIGNQHELDNI
                     DLFNPQCKINYIITVEALKEGWDCPFAYIFCSVQNVSSSKEAEQLLGRVLRMPYAKHR
                     QIEDLNRAYAPLSSKSFGETARNLQDKLIGMGFEALEVAEMLRIPQQENWIGNEGDLF
                     YTPTTVLALTQLPEMSDLSEAKRSQLKITEQEGHILVQVKGNLSEKLEKALIRTATGK
                     KKEQIEQQLQIHQNRVEILSSPAEKGEIFKVIPQLCMNIQGEFDLADSQVLLDYQSWN
                     LLDYPAKLDGFRLQENSQSFEIDVEDKRVSYHFNQQQLSFSDDWLRNPVKRGFSLPLA
                     HQNFYPDFIVELIDGRILIVEYKGEQLKSNEDSKEKALIGNLWEKLSQGKGLFIMAVK
                     SDENGQDVRTQLLNKLA"
     gene            complement(3054..4334)
                     /locus_tag="PXD94_002368"
     CDS             complement(3054..4334)
                     /locus_tag="PXD94_002368"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL6287180.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCRSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLKE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
BASE COUNT         1382 a          766 c          925 g         1275 t
ORIGIN      
        1 ccaaggagag tatttgagcg aagtgcggaa aagaaaaaaa ttcataactt aattagcaga
       61 gctcattatg atatggcaac aaatgaagac tctgctagtg aacatctaga attatttggg
      121 gaggatattg cagaagattc atttgattat cctaaacctg aaaaattaat ctaatttgta
      181 ttagaagcat cgactgaacc atatgatctc gtcctcgata gctttcttgg ctcaggtaca
      241 actgcagcag ttgctcataa aatgaaccgt cgttatatcg gtattgagat tggcgaacat
      301 gctaaaaccc atgttgtccc acggttgaaa aaagtaattg aaggtgagca aggcggtatt
      361 tcaaaagcag taaattggca aggtggtgga tcgtttcgct tttgtgagtt aggcgaggaa
      421 gtatttgacg cattcggttc acttaatccg aacattcgtt ttgacgattt agccgcccat
      481 atttggtatt tagaaaatca tttccctttg caaaaaaaca gcgaaaaatc accgcttgtc
      541 ggcactttca acggcaaagc ctactatttg ttatataacg gaattttagg cgataaacgt
      601 ccgcagagtg gtaatgttct gacacgaaaa ttaatggcgg agttaccata ctttgctgaa
      661 tttattcaac agggaatgga tattgtggta tatggcgaag cgtgccgttt aggagaagtg
      721 cagttagctg aaaagaaaat tacatttaaa cagattccat atgatgtaat gtctcgttaa
      781 tagtaggaga aaaaaatgaa attaaaaaat taccaaaatg aggcattaaa ccgtgtcagc
      841 cactattttt cggcttgttt gcaatctgat gcaaaaaccg catttagcca aattcaacct
      901 gaatatagtt acaagatccc aagtgaacac agtaatttgc gtgatgtgcc ttatgtttgt
      961 gtacgaattc caactggtgg cggtaaaacg ttgttagcct cgcatagtat tgctcgtatt
     1021 gcaaagcagt atttggattg cgatttcccg gttaccttgt ggccagtgcc ttctcgtaca
     1081 attaaaaccc aaactacaga agcattaaaa aatccgcaac acccttaccg tgcagtactt
     1141 gataaaactt ttaaccgaga agtaatggtt attgagtctg aagattttga tttacttcgc
     1201 ccacaagatt ttggtaataa ggcgattgta atcgtttcta ctattcaaaa ttttcgggta
     1261 gaaaatcaag atgggcgaaa aatctatgct tttaatgaaa aattaacagc tcattttgag
     1321 cgtttactac cccatattca ggcaaattta gaaaagattt ccactactga tctacaagag
     1381 aacggtttaa cctccaaaca aacgggcaaa attaagtgtt cttttgcaaa tttgttgcga
     1441 gcttatcagc ctttggtcat tgttgatgaa gcacataatg ctcggacttc actctctttt
     1501 gatgtgctat ctcaactttc cccatcagct attttagaat ttactgctac gccaaacaca
     1561 gatagaaaag caggaagtaa tgtgttgtat cacgtttcgg caagccatct aaaagcagaa
     1621 gagatgatca aattgcctat tgtgctgacc gaacatcaca actggcaaca agcaatagat
     1681 ggggctgtaa tcaatagagg agcattagca caaaaagcac agtatgagag cgagtacgtt
     1741 cgtccgattt attgtttcaa gctgagccga aaaacggcaa agtaacagta gaagtcttaa
     1801 aacagtattt aattgatgag ttaaaaattg atgaagaaga gattgccatt gtgataggta
     1861 atcaacacga attagataat atcgatttat ttaatccaca atgcaagatc aattacatca
     1921 tcacggttga ggcattaaaa gaagggtggg attgcccttt tgcttatatt ttttgctcag
     1981 tacaaaatgt ttcctctagc aaggaagcgg agcagctatt agggcgagta ttgcgaatgc
     2041 cttatgctaa acaccgtcaa attgaagatt taaatcgtgc ttatgctccg ctttcctcta
     2101 agtcgttcgg cgaaacggct cgtaatttac aagataaatt gattggaatg gggtttgaag
     2161 cattagaagt agctgaaatg cttcgtatcc cacaacaaga aaattggatt ggaaacgaag
     2221 gagatttatt ctatacaccg acaacagttt tagcattaac gcaactccct gaaatgtctg
     2281 atttaagcga agcaaaacgt tctcaactta aaattaccga gcaagaaggt cacattttag
     2341 tgcaagtaaa aggtaattta agtgaaaagt tagaaaaagc gttgattcga actgcaacag
     2401 gtaagaaaaa agagcaaatt gagcaacagt tacaaattca ccagaataga gttgaaatat
     2461 tatcttctcc tgccgaaaaa ggggaaatat ttaaagtaat acctcaactg tgtatgaata
     2521 ttcaaggcga attcgactta gctgattcac aagtgctatt agattaccaa agttggaatt
     2581 tattggatta tccagccaaa cttgatggat ttagattaca agaaaatagc caaagttttg
     2641 aaattgatgt ggaagataaa cgggtttctt accattttaa tcagcagcaa ctgagttttt
     2701 ctgatgattg gttaagaaat cctgtaaaaa gagggttttc tttaccatta gctcatcaga
     2761 atttttaccc tgattttatt gtagagctta ttgatggtcg aattttaatt gtggaatata
     2821 aaggggaaca attaaaaagt aatgaagatt ctaaagaaaa agccttaatt ggcaatcttt
     2881 gggagaaatt aagtcaagga aaaggcttat ttattatggc tgtaaaaagc gatgaaaatg
     2941 gtcaagatgt tagaacacaa ctgctaaata aattagcctg atttgcaaat tttatgcaaa
     3001 atataaccgc ttgtggaaac aagcggttaa aatttttcga ttttttgcca atcttatgat
     3061 ttatccattg gctcaaaatc acgtttagta tgtccggtgt agatttgacg tggacgaacg
     3121 attttgatat tgccttggag atacatctct ttccagtgag caatccagcc aacggttctg
     3181 gctagggcaa acattacggt aaacattgag gttggaatac cgatggcttt tagcacgatt
     3241 ccggagtaga aatctacgtt tggataaagt ttatgctcaa tgaaataagg gtcgcttaac
     3301 gcaatacgtt ctaattccaa tgccacttca aatagtgggt tatcaatgtt cagttctttt
     3361 aacacttcgt ggcaggtttc acgcattact ttcgcacgag ggtcgtagtt tttatagaca
     3421 cgatgcccaa agcccattaa gcggaacggg tcatttttat ctttggctcg agcaataaat
     3481 tcaggaatgc gatccacagt gccgatttct ttcaacatat tgatacaggc ttcatttgct
     3541 cctccgtgtg caggtcccca gagagaggca ataccggcag caatgcaagc aaatgggttt
     3601 gcacctgatg aagcggcggt tcgcacggtt gaggtcgaag cgttttgttc gtggtctgca
     3661 tgtaaaatga aaattcggtc taacgcacgc tctaacacag ggttcacttc gtacggttca
     3721 caaggagtgg caaacatcat atataagaag ttgccggcgt aagataagtg gttttgtggg
     3781 aacataaacg gctgaccgat agagtattta taacacatcg ccgcaagggt tgggattttg
     3841 gctaacaaac gaatggcggt taattcacgg tgggcttggt tgtttacatc aatggcgtcg
     3901 tggtagaagg cagccaaagc tccacttacc ccacacatca ccgccattgg gtgcgaatct
     3961 cggcggaaac cggagaagaa tttggttagt tgttcgtgta caagagtgtg ttttttgatg
     4021 agttgaacga agtctttgta ttcttctttg gtagggcgtt cgccgaataa cagcatataa
     4081 ccgacttcta agtaatcaga atgtttggct aattggtcaa tcgggtagcc tcgatagagt
     4141 aaaacccctt catcgccatc aacataagtg atattggatc ggcatacagc ggtcgaaacc
     4201 aatccgggat cataagcaaa taacttatgc tttaagaaag gctgaatatc aatcgtttga
     4261 taccctaagg ttcctttttg catattgagt tcaagttttt cacctgtttc gagtgttaat
     4321 gttgcttttg acatagctta ctcctatt
//
LOCUS       DANLXQ010000179         4323 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.182, whole genome shotgun sequence.
ACCESSION   DANLXQ010000179 DANLXQ010000000
VERSION     DANLXQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.182"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            142..2076
                     /gene="iolD"
                     /locus_tag="PXD94_002369"
     CDS             142..2076
                     /gene="iolD"
                     /locus_tag="PXD94_002369"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL6287181.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            2203..3099
                     /gene="iolE"
                     /locus_tag="PXD94_002370"
     CDS             2203..3099
                     /gene="iolE"
                     /locus_tag="PXD94_002370"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL6287182.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVMAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            3101..4111
                     /gene="iolG"
                     /locus_tag="PXD94_002371"
     CDS             3101..4111
                     /gene="iolG"
                     /locus_tag="PXD94_002371"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL6287183.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
BASE COUNT         1272 a          927 c         1048 g         1076 t
ORIGIN      
        1 gcaataactt cgcagaaatg agaactggat cacatctttt tttcgatttc cttgctatta
       61 taaatgaaat aaatttttca tttgtaaaaa tcagtagaaa accgaccgct tgcaaaagcg
      121 atgtaagcca gaggaggctt tatgcagact actcgtttaa cggttgcaca ggcgttagtg
      181 caatttttgg ataatcaata tttagacgtg gacggcaaag agattaaatt cgttcacggt
      241 gttgccgcta ttttcggcca cggcaatgtg ctgggaatag gtcaggcgct tgctcaaaat
      301 gccggtggtt taacgctcta ccaaggcaga aatgagcaag gtatggcaca tttggcgatg
      361 ggctttgcta agcaaaatct gcgtcaaaga attattgcct gtacttcctc ggtcgggccg
      421 ggggcggcaa atatggttac ggctgcggca acggcaacag cgaaccgtat tcctcttctt
      481 ttattaccgg gcgatgtgtt tgcgacccgc cagccggatc cggtactgca acaaatcgaa
      541 caaccttacg atttaagcat tagcactaac gatgcgttcc gtgccgtcag taaatattgg
      601 gatagagtgc aacgccctga gcagttaatg agtgcttgta tcaatgcgtt tcgtgtgctg
      661 accgaccctg ctgaaaccgg tgctgtcacc attgctctgc cgcaagatgt acaagcggag
      721 agttttgatt tcccgaacag cttcctgcaa aaacgagtgc atcgcttaga gcgtacaccg
      781 ccgacaacag cgatgattaa gcaagcggta acattaattc aaaccaagaa aaaaccgctg
      841 attgtctgcg gtggcggtgt tcgctactcg caagcgggtc aaattctgca aaaatttgct
      901 aaacagttcg gcattccatt tgctgaaacc caagcgggta aaagtgcggt ggtgtccgat
      961 cacgcattga atgtaggcgg agtgggagaa accggttgtt tagcggcaaa ccgtctggca
     1021 aaagaagcgg atttagtgat tggcattggt acacgctaca ccgacttcac gacttcttcc
     1081 aaatggattt tccaaaaccc aaacgtgcag tttttgaaca ttaatgtggc acgttttgat
     1141 gcgtacaaat tagacggcgt gcaactggtt gccgatgcaa aaactgcatt agaggcgtta
     1201 agccaagcca ttcctactga ttatcaagct caatgggaga atgaaatttc acaggcgaaa
     1261 gcggattttg ctgatgaaat tcaacgcctg cgcgctgtgc aatatcaccc tgatttcacc
     1321 cctgaaatta acgataatct tgatcataaa gcggttcaca atgaatttat tcagctcacc
     1381 ggttcccagc tcacccaaac ccaagtgctg ggtattgtga atgaaaccat tgataacaat
     1441 gcgattattg ttgccgccgc cggaagtttg ccgggtgatt tacaacgcac ttggttaagt
     1501 aaaggtgaag agacttacca ccttgaatat ggctactcct gtatgggcta tgaagtgagt
     1561 gcggcagtcg gcgtgaaaat tgcccgcccc gaacaagagg tctatgcctt gctgggggac
     1621 ggctcttact taatggcaca caccgaatta gtgacctcaa ttcaagaggg caaaaaaatc
     1681 aacgtggtgc tgttcgacaa tatggcaaac ggctgtatca acaacctgca aatcggcaac
     1741 ggtatggaca gtttcggtac ggaattccgc ttccgcaatg gcgaaactaa ccaactcaac
     1801 ggcggtttag tgccgattga ttttgccaaa aacgccgaag catacggctg caaaacctat
     1861 cgggtaacta acgaagccga attgcgtgcc gcccttgatg atgcgaaaaa acagaccgtt
     1921 tctaccttaa tcgatattaa agtgttgccg aaaacaatgg ctcacggcta tggctgctgg
     1981 tggcacgttg gtgtcgcgga agtgtcagag agcgagcgta ttcaacaagc ccgccaacgc
     2041 tccgaagagc agttagctaa ggctcggaag tattagttaa aaaaacaact ccccactttg
     2101 agctacgagg ggagaatatc aagcggtcaa atttgcaaaa tttttgataa aaataaccgc
     2161 ttgcatccaa atttatctct ataaatcaaa cgaggtttct ctatgaaagc tgaaaacatt
     2221 caattaggca ttgccccaat cggttggacg aacgatgacc tgccggaact gggagcggaa
     2281 aacactttcg agcagtgtgt gagtgagatg gcacttgccg gctacacggg ctgtgaagtg
     2341 ggtaataaat atccgcgtga tgttgccgtg ttaaaacaca aactggacgt gcgtggcatt
     2401 caaatctgta atgcgtggtt cagcaccttc tttgtggacg gtaaacggga agagaccatt
     2461 gccgagttca tcaaacatcg tgatttcttg catgcaatgg gggcaaaagt gatcggctgt
     2521 tccgaacaga gccgtagtat tcaaggcaca accaaatctg tgttcaaaga aaaaccgatt
     2581 ttcaacgatg aagattggaa acgtatggca gaaggctata acgaattggc gaaacttgcc
     2641 gccgaaaaag gaatgaaagt ctgcctgcat caccatatgg gaaccggtat tcaaacaccc
     2701 gaagaggtcg atcgctatat ggcagaagtg aatgacgatg tctatctgtt gttcgattca
     2761 gggcatttgt actattccga aggctgccaa aaagcgatgc tcggcgtact ggaaaaatat
     2821 atcgaccgta ttatccacgt tcacttaaaa gatgtgcgtg atgaagtcat ggcggaagta
     2881 aaagcaaaag atttaagctt tttagaaggc gtgaaaaaag gcacctttac tgtaccgggc
     2941 gatggtgtca ttgatttccg tccgattttc gatattttag aaaaacacga ctacaaaggc
     3001 tggatggttg tagaagccga acaagatccg gcacttgcca acccgtttga atacgccctc
     3061 aaaggcagaa aatatatccg tgaaaccgca ggagtttaat atgttaaaag taggtattat
     3121 tggtgcaggc cgtattggac gtgtgcattc tgaaagtatt agcaaatatg tcaaaggtgc
     3181 tgaaatcaaa gcaatctctg atgtaaaaat taccgaagaa ttgaccgctt gggcaaaatc
     3241 gatgggtatt ccggaagttt atgatgacta taaaaaaatc ctgcaagatc cggaaattga
     3301 tgctgtattg gtctgctctt ccaccaacac tcacgctccg atttcgattg aggcagcaca
     3361 ggctggcaaa cacgttttct gtgaaaaacc ggtagatgca gacccagcta aaatccgtga
     3421 agtattgagt gcagtcgaaa aagccggtgt aaaattccaa gtgggcttta accgccgctt
     3481 cgaccacaac ttcaaggcga ttaaagaccg tgtggcggca ggcgacatcg gcgaaccgca
     3541 cgttatccga gtaacctctc gtgatccgga agccccgcca atcgaatatg tgaaagtctc
     3601 cggcggaatg ttctttgata tgaccattca cgacttcgat atgattcgtt atttatccgg
     3661 cagcgaagtg gaagaagtgt ttgcagtcgg caccgtatta gtaaaccctg aaatcggcaa
     3721 agcgggcgat attgatactg ccgttatcac gttgaaactg aaaaacggag caatcggcgt
     3781 aatcgacaac agtcgcaaag ctgcctacgg ctacgaccaa cgtgcggaag tgttcggctc
     3841 gaaaggggca atccatatca ctaatgatac cggctcaact gccgtattct caggtgaaaa
     3901 cggcgtggtg gcggaaaaac caaaatactt cttcttagaa cgttatatgc aatctttcgc
     3961 cgatgaaatc agctgtttcg tagattctgt ggtaaacgac aaaccaacgt tagtaaacgg
     4021 caatgacggc ttacaaccgg tacttattgc cttagctgcg aaaaaatcac tggaagaaaa
     4081 ccgaccagtg aaattgagtg aagtggcgta attgatcata aatacaagcg gtcggtttta
     4141 ctcgctacac tcgcacttcg tgccatcggc atagccgatg ttcaaacgca agcattttgc
     4201 agaaaaattt gtaaaactga ccgtttgagt ttttatttat tatcttttgg gaaataaata
     4261 taatcgtgct caatttttat tgattgttta aataaataga aggctggaat tatcaaaaga
     4321 gtt
//
LOCUS       DANLXQ010000180         4322 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.183, whole genome shotgun sequence.
ACCESSION   DANLXQ010000180 DANLXQ010000000
VERSION     DANLXQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.183"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1236)
                     /locus_tag="PXD94_002372"
     CDS             complement(<1..1236)
                     /locus_tag="PXD94_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HDL6287184.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRV"
     gene            complement(1348..1653)
                     /locus_tag="PXD94_002373"
     CDS             complement(1348..1653)
                     /locus_tag="PXD94_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6287185.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            complement(1681..2895)
                     /gene="floR"
                     /locus_tag="PXD94_002374"
     CDS             complement(1681..2895)
                     /gene="floR"
                     /locus_tag="PXD94_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HDL6287186.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSVLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNSDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            complement(3172..3618)
                     /locus_tag="PXD94_002375"
                     /pseudo
     CDS             complement(3172..3618)
                     /locus_tag="PXD94_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
     gene            complement(4087..>4322)
                     /locus_tag="PXD94_002376"
     CDS             complement(4087..>4322)
                     /locus_tag="PXD94_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL6287187.1"
                     /translation="QRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIEDPIVIKQ
                     ILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
BASE COUNT          916 a         1333 c         1248 g          825 t
ORIGIN      
        1 caccctgacc ttgttgcccc tgccccgttt tgccggcgtg accaacgccc ggtgccgact
       61 gttgggtgcg aacaccccgt ggaagcgggt taggttgact ctgggcttcg gtaccagggc
      121 ggccagcctt gcaatgaaat ccaatggttc gaaaatgacg tgcgtggtgc cgtcccggta
      181 cggcgtcttg agctggtagc gcacgttgcc gcctcgtgtt aacgacagcc gcttctcgga
      241 taccgccggg cggctgatgt accggcacag ccgttcgagc ttcttgcgtt catcggccct
      301 ggccgccacg ccggcgtgca ggctggaccc ggctaccttg ccaatcccgt caccgaacgg
      361 atcaccactg gtcggcagag tttgcaaagt gaacaccttt cgccccgcct gtgaaccgac
      421 agcgatacgg taagtgatcg agtgccccag caggggtgtc atcgggtcgt catccaccgc
      481 atccgaggcc agatagctgt tttcgacatc ccgttccagc aggccttgcc gttccagata
      541 gcgacccacc cggtgggcga tggtgtgcgt cagctgggtg agctctgggc tggtcggcgc
      601 cttgacccag cggaaacgcg ctgagccgtg ggattgctcg acatacacac cgtcgagaaa
      661 cagcatgtgg aagtgaacat tcagattgag cgccgatcca aaacgctgga tcagggtgac
      721 cgcgcccgtc ttggccactt ggtgggtatg gcccgctttc ttgaccaggt gcgtggcaat
      781 gacgcggtaa acgatgccca gcacccaccc catgatctcg ggccggctgg caaacaggaa
      841 acgcagctga aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc
      901 atcaaccagc aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc
      961 gcgacgctta cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg
     1021 tagaaagcca tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata
     1081 gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc
     1141 aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg
     1201 atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg
     1261 tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg
     1321 ttgctagaca ttgccagcca gtgcccgtca ctccgcggtc ttcactgcgt gatcgagttg
     1381 atcgacaccc gccgtgacac gctccatgaa gtgcctgcct gcgtctgtta gccgaacgcc
     1441 ccgcgcatgg cgctcaaata gcaggacacc aaggttatcc tccagcgctt tcacacgcgc
     1501 gctgacgctc gactggctga taccaagtgc cttggccgca tgccgaaaat tcagatgctc
     1561 ggcgacggcg atgaactgaa caagggaaat gagcggtatc ctgccagaca ggataccgac
     1621 attcacgagg tttcgatggt tagtgcgccg catcggagcg ggcctgctac cagtcgtcgg
     1681 ttagacgact ggcgacttct cggtggcagc cccacggagc cgaaggagca ccagccccaa
     1741 cgaaaccagt accgccatcg ccgtggcgta acagatcacg ggccacgctg tgtcactgtt
     1801 taaaagtgcc accgccaatg tcccgacaat gctgactatc aggctttgaa cgcagaagta
     1861 gaacgcgacc gctgatcccg cgatgtcgtc gaactctgcc aaagcgccgt tcgcggtaac
     1921 ggacaccgtg aagacaatac cgaccgcgac aacccacatc ggtaggatga aggtgaggaa
     1981 tgacggcgag ccgtaaagtt cgccgatccc caacaggacc gctccgcaaa caagcaacgc
     2041 catcccacgc gccacgcatc ctgcgatgcc ccatctggcg acaaaggact tcgcgaaacg
     2101 ggttgtcacg atcattacaa gcgcgacagt ggcgaaggca aagctgaatc cgatctcgga
     2161 atattccgct tggcctatga gcacacgggg agccgtcgag aagaagacga agaaggtgcc
     2221 cataccggcg ctaaagccga cagtgtaaac ccaaaaagcc ggactcgcga agatcggcaa
     2281 gacagatcgg cgcgtcttga cttgatccag agggcgggtt tcgtgccacc tgaaacccgc
     2341 atttaggagt gcgagcatcg ccagtatagc caaagtaatg aatatcgcct gccatcccaa
     2401 gaactcgccg atcaatgctc cggcgatagg gccgagcgca ggcacgaacg ccagcaccga
     2461 actgaaaagg ccgtagatga cgacaccctc aggacggttg gcataaacgt cgcgaaccgt
     2521 cgcgaacgtc gccaccagca tggccgacgc gcccactgct tgaagtagac ggaaagcgac
     2581 aaaggccggt gcagttgaag accaagctgc tcccagagac gcaatgacga aagccgttgc
     2641 gcccgcaagt agaattggcc gtcgcccgat tctgtctgag agcggaccaa aaatcacctg
     2701 gcccacgccg agcatcacca tatagaggct caacgtgagt tggatcatag cgggcgtcgt
     2761 gttcaggatg ccgggcatcg ctggaacgac agggagataa atatccatcg ccagtgaagc
     2821 gaggatgtcg aaaggagcca tcagcagcag tgctgccggc agcgtatagg cccacgcggg
     2881 gcgtgtggtg gtcatgaata tgatctcctt gcgattgata tttgtttagg cccacgcggg
     2941 gcgtgtggtg gtcatgacga atcaaccctc gattaaggaa taccgggcga cgtctgctcg
     3001 tcagcaatca gatgagacta gccttacaga gcgccgcaac aacaatactg gttgttgcgg
     3061 cttacttgtc tgctgacttg gaatttccca tctgattact ccacgcttac gaatatgaat
     3121 tgctacattt tatccgatta tcttttgctt cgcaatgcgg catgggcgca ctcagcgttc
     3181 cagccccctc tgccgcccta actgccacga caccgacccg ccctgcacga tgcccataac
     3241 ctcgcggccg agttggcggt cgatgatcgg ccgccacggg acaagggtga actcatggga
     3301 tttctcgacc acggcgaact tgccgctcga tagatgcacg gttccggtaa acttgccgct
     3361 gacgctctcg ccgtccgtgg cggcgcggaa cggcaggccc ttactcaaag ccatatccga
     3421 tccgacgccg gctacctcgc gctcccgcag gatggcgaga aggttgcgcc ggtagaagac
     3481 gcggctgtcc cggctgcggg tggcgtcgcc ctgttcgata tggtgctcgc ggcgctggtc
     3541 catggcttcg cggacttgtt gcccgaagcc ggttggcgca aggtcggccg tctcgccgtg
     3601 gatcagccgc cggtccagcc aggtcgcgcc gtccgatccg atctgtttgt tcagatcgac
     3661 cggggaaagg acgcgaacgc tggcctgact gtctcggccg gcgtcatggg cggcggcacg
     3721 gctgaccaga tcatcgggga tgcgccattg gtcggcgtcg atccgctcga cgataccggc
     3781 ccggcgtagc gcctccagcc ggcgcacatg ggcatcgaca tagccctcat agtcgccgcc
     3841 tggaacgcgg cacgtatagg aagaataaac gcccttttca cccaagtcca acagctttgg
     3901 accgcagttg actctttcga cacccctgcg atgcaaccca atccggctga cggggagcca
     3961 gcaacgctga aaatttaccc tcctctttcc cactagcggc tccttttccg acaaccagca
     4021 cggcggatcc ctgccgcggc gctgtgaacg cagcattttg attggtatcg ttggccttca
     4081 ggctcgtcag tcaaacagac ccaggagcag ctcagccggt ggcgcccggc tttcgggtaa
     4141 cgccctggtc ccgctggttt cggctttgtg cttcaggtga tcaaggatct gcttgatcac
     4201 tatagggtct tcaatgcagg cgatgacttt catggcgccg ccgcagccgc tgcaggtctc
     4261 gatgtcgata ttgaaaacac gcttgagccg ttgcgcccat gtcatcgacg ctcgccgttg
     4321 tg
//
LOCUS       DANLXQ010000181         4256 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.184, whole genome shotgun sequence.
ACCESSION   DANLXQ010000181 DANLXQ010000000
VERSION     DANLXQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.184"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(47..122)
                     /locus_tag="PXD94_002377"
     tRNA            complement(47..122)
                     /locus_tag="PXD94_002377"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Asn,seq:gtt)
     gene            complement(238..987)
                     /gene="moeB"
                     /locus_tag="PXD94_002378"
     CDS             complement(238..987)
                     /gene="moeB"
                     /locus_tag="PXD94_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL6287188.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(1086..2315)
                     /gene="moeA"
                     /locus_tag="PXD94_002379"
     CDS             complement(1086..2315)
                     /gene="moeA"
                     /locus_tag="PXD94_002379"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL6287189.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPK
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGN
                     VDAGEKVTIQPFNSLMY"
     gene            complement(2337..2954)
                     /locus_tag="PXD94_002380"
     CDS             complement(2337..2954)
                     /locus_tag="PXD94_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL6287190.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHLIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(3022..4233)
                     /locus_tag="PXD94_002381"
     CDS             complement(3022..4233)
                     /locus_tag="PXD94_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL6287191.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQESRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
BASE COUNT         1300 a          991 c          784 g         1181 t
ORIGIN      
        1 tataaaagga gacaaataaa acaagaacaa aagaaaaata aaatcttggc tcctcctgcg
       61 ggactcgaac ctgcgacata tggattaaca gtccaccgtt ctaccgactg aactaaggag
      121 gaattaacgg tgtaaataat aggctaaaac gttttagccg tcaaataaaa aaaataaact
      181 gtcgttcatt ttttacattt cttgacattt tttcgccaat aagtatgaga aaaatactta
      241 ataagaggta caaaccggac agttattttg cttaggtaat gccatttctc tgacagaaaa
      301 atgcaagccg tcaatcatta acaattttcc ggaaagagtt ctgccgatat taagtagcac
      361 tttaattgct tccaacgctt gaatactgcc tatgatgcca acaatcggag caatgacccc
      421 cgcttcaaca caacttaaag tcgcatcacc aaacaacctg cttaaacatt catagcacgg
      481 ttcttgttct tgataacaaa atacactcac ctgcccttca aatcgaattg ccgaaccgga
      541 aacaagcggt cgtttttgct taaaacattg caaatttaac tgattacgca cagtaacgtt
      601 atcggtacaa tccagcacta aatctacatt tgcaatcagc ttggctaact gctcatcgga
      661 acatcgctga ttgatagttt caatctgaat gttcggatta atcgccgcta atcgagcttt
      721 ggcagattcc acctttggct gcccgatagt cgcatcggta tgtaaaattt ggcgttgcag
      781 attagaaagt gagaccagat caaaatccac caaagtcaaa tgcccaacgc cggcagaagc
      841 taagtattgc gatgccgcac agcccaaacc acctaaaccg acaatcaaca ctcggctggc
      901 ttttaacttt tcctgcccgt caaaatccac gctttttagg ataatctggc gattataacg
      961 tagcatttcg tgatcggtaa gttccatttt tacacctaaa gagtaaacga tggcgcaagc
     1021 gtccacgctt gtgcctatat ttttattgct agcacaagcg tggacgcttg cgctatctta
     1081 attcattaat acatcagcga attaaacggc tgaatagtga ctttttcccc agcatcaaca
     1141 ttgcctcgtt ctgcttctag cacaataaaa cagttacttt cattaaaagc gctgaaaata
     1201 tgtgagcctt gcgtgccaac tgcacgaact tctaactcac ctttttcatt ggcatagtaa
     1261 tagccccgtt ggaaatcttg gcgaccgacc gcttttttca attttccggc actgcaagcg
     1321 gttagatttt gggttaattt tgcaatttta tctgtcgata aacccgaaag tttggctaac
     1381 gccggttgcg ctaattggta gaaagtcact aatgcagaga cagggttgcc cggcagccca
     1441 aaaaaccaag cctgttctaa tttaccgaat gcaaaaggct tgcccggctt catcgcaatt
     1501 ttccagaaac cgatttcgcc taatttttcc agtacatctt tggtaaaatc tgcctctccc
     1561 acagatacgc cgccactggt aatcaacaca tcggcttgct gttgggcttg cctgaaagtt
     1621 tgctcaaaca gaattgggtc atcaggcaaa atcccaaaat ctaaaatctc gcagttgagt
     1681 ttttccagca ttaaacgcac cgcaaaacgg ttggtgtcgt aaatttttcc ctcactcaaa
     1741 ggctcgccaa ctgaggttaa ttcatcgccg gttgaaagaa ttgccacttt taattttaca
     1801 aaaacaggca cagaagcaat accgagtgaa gctagcaacg gtaaactggc tacattcaat
     1861 aatgagcctt ttttcaagac caatgccccc tgtttaacat cttccccaat gcggcgaatg
     1921 ttactgccta attttggaat atggttaaag gttacagagc catcggaatt taacgtagtt
     1981 tcctcttgca ttacaacggc atcaacgccc tttggcacta atgctcccgt cataatccgc
     2041 acgcattcgc ctgcctttat ttcgccgtta aacgggttac cggcaaaggc tttaccagcc
     2101 acattcaagg ttaaattttg tgctaaatct gaaatacgaa ccgcgtagcc gtccatcgcc
     2161 gaattatcaa aattcggtac attaatcggg gaaaaaatat cctctgccaa cactcggtta
     2221 gcacactcat ttaacggaag ttgttccacc aatttaggtg tcgataaacg agcaagcata
     2281 ttatctaacg catcactcaa agccaaaagt gccataattt cctacctatt caacgtttag
     2341 ctaatttgta caaagaaatc ccgtacgcca atcaacagat tattcaccgc aatcgcagcc
     2401 agaattaacc ccatcactcg gctgataatc gcagcccctg cattaccaat aaggtgctga
     2461 atacggtttg ccgctaatag caacaaatag gtaataaaga gtaccaaaag cataatgccg
     2521 gcggtgataa tttgatcgcc gatatgaaaa cggtgattgt cggttaataa tacgattgcc
     2581 atcatcgccc cgggagaagc aatcgacggt acagctagcg gataaaccgc caattcattc
     2641 agattactgc tgatccgcat ttcttgctcg ggcttgcttt cgccaaaaat catcgttaaa
     2701 gcaaataaca acagcactaa gccgcctgcc gcttggaatg caggtaacgg aatctgcatt
     2761 gattctaaca gcacttgccc accgataagg aagaaaatca aaataccggc ggcaatcaag
     2821 accgcattta gtgcaatttt acgccgtgct tcaatcggca aaccaatggt ttttgccaaa
     2881 taaacaggaa cagaaccaat cgggtcgatc accgcccata aaaccacaaa ttgtaccgct
     2941 aatgaatcaa acatattctc tcctgttatc ttgataattt gcaaaatttt agcataaaac
     3001 aaccgcttgc acggcttttt cttacagata gctcaccggt aacgctacca aattttcact
     3061 taaataaagt ttggtcggat attggttaat aatcgctccc tgccctaatt tccattcact
     3121 agaaactgag tttagcaagt caaaactttt tgccattttt ttggttgggc ttgccgtgac
     3181 cttaatttct atcggataaa gcgtgcgatc atattcaata attaagtcga tctctttctg
     3241 gttagtatca cggtaaaaat aaatcggcgg ctcaattcct tgattatgaa aggattttat
     3301 aatttggctt accacaaagg tttcaaagaa ctgccagctt tttgcccctt gagcaatagt
     3361 ttcgggggta agccatttgg ttaaatacgc cattaacccg gtgtctagaa aatacacttt
     3421 tggggtctta attgcccgtt tcaaatgatt gttgtggtat ggctggagca aatagataat
     3481 gccggaagtc tgcaaaatgg cgacccaacg tttaatcgtt tcattcgata ctccaacttc
     3541 ttgagcaata ttactgtaat tgagtagctc accactacga gaggcaatag agaccataaa
     3601 acgggtaaaa tccgttgtgc tggcaatatt ggttaattgg cgaacatctc gctcaatgta
     3661 ggtcgctaca taagagcgat aataaatctc ccaatccacc tcttgtgcat ataaacgagg
     3721 catatcgcct ttatggataa gctgccagat gttctccggt gattttaatt cttgctcccg
     3781 agctaacaaa tagtcggttg tcggtaaaaa aggttgatcg aacttcaccc ctaaaatttc
     3841 acgcaacgaa agcccttgta attttaaaat agcaatacga ccggcaagtg tttcactcac
     3901 attctgcatt aattcaaagg cttgcgaccc cgaaagtaaa aacaaaccgt tttgctgttt
     3961 tttatcaatt tctaacttta ataacgcaaa aatttcaggc acatactgta cttcgtctaa
     4021 aataagcttg ccttgatggt ttaaaaaaaa caggcgagat tcttgtttag ccagctccag
     4081 caacattggg tcatcaaaag taatatattg atagccctca ccaatatgtt gtaacaatgt
     4141 cgatttacca acctgtcttg cccctgtcac taaaacggca ggaaattgcg ccacgagtcg
     4201 ttttaacagc gattgcattt gccgagaaat catttttcct ccaaaatagc ctaaat
//
LOCUS       DANLXQ010000182         4245 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.185, whole genome shotgun sequence.
ACCESSION   DANLXQ010000182 DANLXQ010000000
VERSION     DANLXQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.185"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            12..1559
                     /gene="murJ"
                     /locus_tag="PXD94_002382"
     CDS             12..1559
                     /gene="murJ"
                     /locus_tag="PXD94_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL6287192.1"
                     /translation="MIVSFMTLISRILGLIRDIVVATILGTGVSADIFLFANRIPNFL
                     RRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTLVAMIASPVV
                     AALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVLNTIGKFGVM
                     AFSPVLLNVAIIAVALWGRDYFDSPDIALAWGVFLGGLLQFLFQIPFMKKEGLLVKPK
                     WAWKDEGVTKVRKLMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYYADRLIEFPL
                     GLFGIAISTVVLPSLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGIPAMIGMAVL
                     AQPIIMTMFMRGKFGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYANQNTKTPVKV
                     GIIAALSNICFGLAFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYYKVSHKTVLF
                     VLKLFIAACVMGALVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYFASILVLGIR
                     KKDFRSA"
     gene            1561..2028
                     /locus_tag="PXD94_002383"
     CDS             1561..2028
                     /locus_tag="PXD94_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL6287193.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(2093..4102)
                     /locus_tag="PXD94_002384"
     CDS             complement(2093..4102)
                     /locus_tag="PXD94_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL6287194.1"
                     /translation="MDLIKKLNTGSTFRVPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFDQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
BASE COUNT         1205 a          845 c          948 g         1247 t
ORIGIN      
        1 taagatcagg aatgatcgta agctttatga ccttaatttc tcgcatactc ggtttgattc
       61 gagacattgt ggtggcaacc attttaggca caggcgtgag tgccgatatt ttcctgtttg
      121 ccaaccgtat tcccaatttt cttcgccgtt tgtttgccga aggggcattt tctaaagcct
      181 ttgtgccggt gttggcggaa tataatgcgg ataacgatcc aaacaaaacg agagaattta
      241 ttgccaaagt atcgggtaca ttaggaggtt tggtaactgt tgttaccctt gtggcaatga
      301 ttgcctcgcc tgtggttgcc gctctttttg gtacaggttg gtttttagat tggctttacg
      361 acgggccaaa tgccgaaaaa ttcacccaag cctctttttt gctcaaaatc actttccctt
      421 atttgtggtt tattaccttt gtggcgttat ccggtgcggt gttaaatacc atcggtaagt
      481 ttggggtgat ggcattttcc ccggtgttgc tgaatgtggc gattatcgcg gtagcgttat
      541 ggggcagaga ttatttcgat tccccggata ttgccttagc gtggggggta tttttaggcg
      601 gcttattgca atttttattc caaattccat ttatgaaaaa agagggctta ctggttaagc
      661 caaaatgggc gtggaaagac gaaggtgtca ctaaagtccg taaattaatg attccggctt
      721 tatttggcgt ttccgttacc cagctaaatt tactgctaaa ccaagtgatt gcctcgtttt
      781 tgattaccgg ctcgattagt tggatgtatt atgccgaccg cttaattgaa tttccgctcg
      841 gtttatttgg aattgcgatt tccaccgtcg tgctgccaag cctttcccgt attgcgaaaa
      901 acaaagaact cactgaaatc caacgtggag aacactttca aaacacaatg gattgggggg
      961 tgagaatggt gctgttgctc ggcattccgg caatgatcgg tatggcagtg ctggctcagc
     1021 cgattattat gacaatgttt atgcggggta aatttggttt tgaagatgtg ctggcaacct
     1081 cttatccact ttgggtaatg tgcttggggt taaacagcta tatgttgatt agcgtgctgg
     1141 caaacggttt ttatgccaac caaaacacca aaacaccggt aaaagttggg attattgccg
     1201 ccttaagtaa tatttgcttt ggcttggcgt ttgctccgtt tttgggatac attggtttag
     1261 cgttagcttc tgcgtgttcg gcattagtga atgtgagctt gctgtatgtg aatctctcaa
     1321 aaaatggtta ctataaagta agccataaaa ccgtgctttt tgtgttgaaa ctattcatcg
     1381 ccgcttgtgt aatgggggcg ttggttgcct atttcacgcc tgagattgaa ctttgggcat
     1441 taatgacaat gtggcagaaa gtgtattggc tgatttggct gatgatctta gccggcggta
     1501 gttattttgc ctctattttg gtattaggca ttcgtaaaaa agatttccgc tcggcataaa
     1561 atgaaatcat taaaactgaa aatcccgccg ccggcttggt tcttgctttg tgccgcttta
     1621 atgtggctgg tagctcaata tgtgccggtg agcttgccga attatcagca tacagccata
     1681 ttttttagcg gtttagctgt ggcgataatt ggcggagcgg ttgccgtctc ggcactttgg
     1741 gtcatacacc aagcgaaaac gacatacagt ccttttcgac cacaacatac cgctaaattg
     1801 gtcacttggg gcatttaccg caaaagccga aacccaatgt atctttcgtt actattaggt
     1861 ttgattgcgt gggcattatg gttgggggca atgttggcgt ggctggtttt accgctgttt
     1921 gtatggttga taacctattt ccaaatcaag ccggaagagc aaattttagc ccaaaaattc
     1981 ggcacacatt atgccaaata ttgcaaccaa gtaaggcgtt ggctttaaat gcaagcggtg
     2041 agattttgtt ggaaatttgc aaatttttcc gaaaatctca ccgcttgtgg ggttagtgat
     2101 tgaaatcagc cgtcattaag tcgtgcgagt tgtccatcgc taaattgata aagcgttcat
     2161 actgttgcag cacgtcggca atcaactctt ctttggtcat atattgcaca tcataacctt
     2221 tccgcccgtc taagaaataa gtaatcggct cgtaaatggt attttgctca atgttcggta
     2281 ggttgtcgtc acctaccacc aaatccgata attcccgtgg cacactttca atgccgtaaa
     2341 tgaaattgcg taaattttct ttcaccactt caaactctaa tttcgggttt tgttcgttgg
     2401 tgaaattcat tttagcgatt aagccatagc tttcaaattc ctcgataagt tctaaaaatg
     2461 ctggtctggc aactttaata aagaagtgac ggacatcttg cttattgctt gggttgatga
     2521 ttttctccaa gcgttgtttc caatctttac ccgcccaatg ttggctacct tgcgagaatt
     2581 ttttgttgaa gtattggtta tctaccatta atcctttcca taagccgata cagagaatca
     2641 gcataatgaa ggtaaatggt aaggcgataa tcagtgtcat tgtttgcaga gaagccaagc
     2701 cacccgaata gagtagtgat aacgctaaga tggctaataa tgctccccaa agcacgcttt
     2761 gccatttcgg tgcattttct tcgccttgtg aagcaatgct gttgagaaca aaaatccccg
     2821 agtcggcaga ggtgataaag aaaatactga taacaatgac ggcaacgaaa gaggcaatag
     2881 agccaaacgg taattgctca aaaaaggtaa ataacagttg ttcggtattg ccgctgactt
     2941 cagctaaagc accggcagtt tgttgatcga accaaatggc agagctgccg aagctggtca
     3001 tccataaaat gttaaacagc gatggaacaa atagcacccc taaaataaat tcacgaatgg
     3061 ttctgccttt agagatcttg gcaataaaca aaccaacaaa tggcgcccaa gatgcccacc
     3121 atgcccaata aaggaccgtc cagccgctta gccagccttg atgttccggt tcataagcga
     3181 aggtacggaa actcatctca agcagcgagc taaaataata gcctaaattt tcggtgaagc
     3241 ttgagaaaag taatagagtt gggccggtga ttatcacaaa aataagtagc aatccggcta
     3301 atgtgagatt gatttcactt aagattttaa cccctttgcc tacgcccgaa acggcagata
     3361 acacggcaag agccattgct actgttacga taatcgcaag aacagtaaaa ttgctctgtt
     3421 caattaaacc tagattgtta aagccagcac tgacctgcat caccccaaag ccaagtgtag
     3481 tcgttaaacc gaaaatcgtg ctacaaaggg caataatatc aattaaatgc ccccagaagc
     3541 ctgaaatacg atgttttagt aagggataaa atccggaacg aatagtgagt gctaacttat
     3601 atctgaagcc aaaataagca agagctaagg caatcacacc ataaattgcc caagcgtgaa
     3661 taccccaatg atagaacgtt gtcatcatcg cttctttcat acgctccggc tcagttaaat
     3721 tttgttggac gggtttaagg taatgcaaaa taggttctgc taccccaaaa tacattaacc
     3781 cgatccccat tccggcggcg aataacatcg caatccaaga gccaaaacca aattcaggct
     3841 catcggtatc tgcccctaat ttaatatcac ccaatcggct gaaagagaga aaaattagaa
     3901 acaggaaaaa gatagagcct gcaaaaatat agaaccagct aaaatgttgg aagagactat
     3961 ttttgatggt atcaagtgag gtttgtgctt gctgtggaaa gatgatacag aaaacggcaa
     4021 caaagctgac aaagagtaaa ctcggtagga aaatcggtac cctaaaggta cttcctgtgt
     4081 ttaatttttt gattaaatcc aatgctattt cctctatttt tgaatggtta gaggcttgaa
     4141 tatagcaaaa agatataaaa ataacaaatt taagacgtat tggtaataat attgcaacct
     4201 aatgggtttg gtaaaaaaat cgggaaattt gaccgcttgc acatt
//
LOCUS       DANLXQ010000183         4179 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.186, whole genome shotgun sequence.
ACCESSION   DANLXQ010000183 DANLXQ010000000
VERSION     DANLXQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.186"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            9..1217
                     /locus_tag="PXD94_002385"
     CDS             9..1217
                     /locus_tag="PXD94_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL6287195.1"
                     /translation="MSKNLILILNCGSSSLKFAILDPKTGDEKLSGLAEAFHLEDARI
                     KWKLNGEKGNADLGAGAAHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVV
                     ITDEVIKGIEDATQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAF
                     LYALPYTFYKENGIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVV
                     RNGKCIDTSMGLTPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLG
                     LTGVTSDCRYAEDNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGG
                     IGENSGLVRELTLNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELV
                     IAQDTAKLAL"
     gene            1270..3405
                     /gene="pta"
                     /locus_tag="PXD94_002386"
     CDS             1270..3405
                     /gene="pta"
                     /locus_tag="PXD94_002386"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL6287196.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            3519..>4179
                     /gene="can"
                     /locus_tag="PXD94_002387"
     CDS             3519..>4179
                     /gene="can"
                     /locus_tag="PXD94_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL6287197.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEERSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KI"
BASE COUNT         1301 a          781 c          860 g         1237 t
ORIGIN      
        1 atttgaccat gtctaaaaac ttaatcttaa tccttaactg cggtagttca tctcttaaat
       61 ttgctatttt agatcctaaa acaggtgatg aaaaattatc tggacttgct gaagcttttc
      121 atttagaaga tgcacgtatc aagtggaagt taaacgggga gaaaggtaat gctgatttag
      181 gtgcaggtgc cgcacatagt gaagcactta cctttattgc tgaaaaattg ttatctgacg
      241 aattaaaagc aggtatcggc gcgattggtc atcgtattgt tcatggcggt gaaaaattca
      301 cctcatcagt tgtaattacc gatgaagtga ttaaaggtat tgaagatgca acgcaatttg
      361 caccattaca caaccctgcg catttaattg gtattaaaga agcattccgc attttccctg
      421 aattaaaaga caaaaacgta gctgtatttg acactgcatt ccatcagaca atgccggaac
      481 acgcattctt atatgcactt ccatatacat tctataaaga aaatggtatt cgtcgctacg
      541 gtttccatgg cacaagccac ttctatatca cctcacaagt tgcagaatta gcaggtaagt
      601 ctgttgagca aaccaatgcc attatttgcc acttaggtaa cggtggttct gtttctgttg
      661 tacgcaatgg taaatgtatt gatacctcaa tgggtttaac tccattagaa ggtttagtaa
      721 tgggtactcg ctctggtgac ttagaccctg cgattatctc tttcttatac aataacttag
      781 gtatgtcaat ggacgaaatt gatactactc tagtaaaaaa atctggtctt ttaggtttaa
      841 ctggcgtaac tagtgactgt cgttatgcgg aagataatta caatgacgta tcaaaaccgg
      901 aagcaaaacg tgctttagat gtatatagct atcgtttagc taaatatatt ggttcatata
      961 tggcaatttt aggcgatgac cacttagatg ctatcgcatt tacaggtggt atcggtgaaa
     1021 attcaggctt agtacgtgaa ttaaccttaa atcacttaaa attatttggc attaagttag
     1081 atcaagagcg taaccttgct gcccgttttg gtaaagatgg agtcattaca gcggacgagt
     1141 caacatttaa agctgttgtt atcccaacta atgaagaact cgtgattgca caagataccg
     1201 ctaaattagc attataatca tagggggcag atattctgcc ctttcacttt cttttaattt
     1261 ggaaatatta tgtctagaac gatcattctt atccctattt caactggtgt tggtttaacc
     1321 agtgttagct taggtttagt acatgctttg gagcaaaaag gttctaaagt aggattttta
     1381 aaaccgattt ctcaacctat cagcggtgaa gatacgttag accgctcaac aacgattatc
     1441 agagcagaac aaacaacaga ggttggtgag ccgtttatgt taagcgaagc tgaaacatta
     1501 atcggtcaaa accaaacgga tgtattgtta gaaaaagtgg tggaacgcca ccaaaaatta
     1561 tcaaaaaata atgaaattgt cattgttgaa ggtttaattc caacgcgtaa aaactcatac
     1621 gctaacagca ttaactatga aatggctcaa gcccttgatg ctgaaattgt attagttgca
     1681 gcaccggcct cagatacacc agctcaatta aaagagcgag ttgaagctgc cgcttcacaa
     1741 tttggtggac gtaacaaccc aaatttactg ggcgttatta tcaataaatt caatgcacca
     1801 gtagatgatt caggtagaac ccgtccagat ttgactgaaa tttttgattc atttcaacac
     1861 tcaaataata atgtttcaga agtcgagcat ctttttgcta aaagtccaat taagctgtta
     1921 ggctgtatta gctggaaagc agaacttatt gctacacgag caattgatgt tgcaaaacat
     1981 ttaggcgctt caattattaa tgaaggcgaa ataaaggctc gtcggattcg cggtgtgaca
     2041 ttctgtgcac gtaacttacc aaatatggtc gatcatttta aagccggtag tttattagtt
     2101 acctctgcgg atcgtcctga agtgttagtt gctgcatcgt tggcggtaat gaatggcatc
     2161 gaaattggtg ctattttact aaccggtggt tataaaattg atagctcaat tgcaaaactt
     2221 tgccaacaag catttgaaac cggtgtgcct gtattccgta ttgaaggtaa cacttggcaa
     2281 accgcattaa gcctacaaag cttcagctta gaagtgcctg tagatgataa agagcgtatt
     2341 gctgcaatta aagaatatat ggcaagcaaa tttaatgtag gtttcattga tgaggtttct
     2401 aaagccgcta ctcgtgcaag acgtttatca cctgcagcat tccgttacca attaaccgaa
     2461 tatgcccgtc aagcgaaaaa acgcattgtt ttacctgaag gagatgagcc tcgtactgtg
     2521 aaagcggctg cattatgtgc tgagcgtggc attgcagaat gtgtgttatt agctaaccca
     2581 acagacgtac aacgtgttgc tgaagcacaa ggcgtggtat taggtaaagg cgtaacgatt
     2641 attgaccctg cgagcattcg tgaaaattat gttgatcgct tagttgaatt gcgtaaagca
     2701 aaaggaatga ctgaagttgt tgctcgtgag caacttttag atacagttgt attgggtaca
     2761 atgatgcttg aagctaatga agtggacggt ttagtctcag gcgcagttca cacaaccgct
     2821 aacactattc gcccaccaat gcagatcatt aaaactgcac cgggaagctc tattgtatct
     2881 tctatcttct ttatgctact acctgatcaa gtattggtat atggtgactg tgcggtaaac
     2941 cctgacccaa ctgcagaaca gctagcggaa attgcgattc aatcagcaga atctgccaaa
     3001 gcctttggta ttgaccctcg tgttgcaatg atctcctact ctaccggtac atcaggttca
     3061 ggtgcggatg tagaaaaagt gaaagaagca actcgtattg cccaagaaaa acgtccagat
     3121 ttaatcattg atggcccatt acaatatgat gcggcgatta tggaagatgt tgcccgctca
     3181 aaagcaccaa attcacctgt agcaggtaaa gcaactgtat ttgtcttccc ggatttaaat
     3241 actggtaata ccacctataa agcggtacaa cgttcggcag atttagtctc tatcggccca
     3301 atgttacaag gtatgcgtaa acctgtaaat gacttgtccc gtggggcttt agttgacgac
     3361 atcgtttata ctattgcgtt aactgcgatt caagcaacgc aataattccc tcccaaatga
     3421 aaccaaatgc ccttaaaact aagggcattt ttatttttta cctacccgac ttttcaattt
     3481 aatcatataa tcaagattag tgttaaaaag gagaatttat ggaaaaaatt gaacgcttat
     3541 ttgccaataa ccacgcctgg gcaactaaga tgaaagatga acaatcggat tattttaaag
     3601 aattagcgga acatcaaaag ccaacctatt tatggattgg ctgttccgat agtcgtgtgc
     3661 ctgctgaaaa actcacaggg ttaggccccg gagaattatt tgttcatcga aatgtagcaa
     3721 atatggtgat tcacactgat cttaattgtc tgtcggttgt tcaatatgcg gtagatgtat
     3781 tagaaattga gcatattatt atctgtggtc acactaattg tggtggtatc aatgcggcaa
     3841 ttgcacaaaa agaacttggt ttaattaata actggttgtt acatattcgt gatatctggt
     3901 ttaaacacag ccatttacta ggcaaacttc cctatgaaga gcgatcaaat gtcttgaccc
     3961 gattaaatgt tgcagaacaa gtttataatt taggtcagtc ctctattatt aaaaccgctt
     4021 gggaacaggg taaatccctc tcgctacacg gttgggtata cgatgttaca gatggtttct
     4081 tgattgacca aggtgttatg gctactagcc gagaaacatt agaaattacc tatcgtaatg
     4141 caatagctaa aataacaacc gaagcaaacg aaaaaatag
//
LOCUS       DANLXQ010000184         4127 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.187, whole genome shotgun sequence.
ACCESSION   DANLXQ010000184 DANLXQ010000000
VERSION     DANLXQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.187"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(230..1870)
                     /gene="pgi"
                     /locus_tag="PXD94_002388"
     CDS             complement(230..1870)
                     /gene="pgi"
                     /locus_tag="PXD94_002388"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL6287198.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINEKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1903..2151)
                     /locus_tag="PXD94_002389"
     CDS             complement(1903..2151)
                     /locus_tag="PXD94_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL6287199.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2275..2319
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2323..2991
                     /gene="queC"
                     /locus_tag="PXD94_002390"
     CDS             2323..2991
                     /gene="queC"
                     /locus_tag="PXD94_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL6287200.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2984..3406
                     /gene="queD"
                     /locus_tag="PXD94_002391"
     CDS             2984..3406
                     /gene="queD"
                     /locus_tag="PXD94_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL6287201.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3406..4032
                     /locus_tag="PXD94_002392"
     CDS             3406..4032
                     /locus_tag="PXD94_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL6287202.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
BASE COUNT         1253 a          757 c          875 g         1242 t
ORIGIN      
        1 tgagtgaaga gtagaggtaa aatagtaagc ctttttaacc agtttattta ttgtacctat
       61 tttagaaaaa agaaaagtag aaaagagata ttaggtaaac tcatagtgca aaagaatata
      121 gaaaaaactg cttgtgtaga aatggcgctt agttcagtcc tcagctaaaa ttttagaaaa
      181 aaagcataga gattttttac ctctatgctt tttattgtat tgactatatt taacgccaag
      241 ccttgaattg gttaattaaa ccatttgtgg agctgtcgtg gctgttgatt ttttcatcgg
      301 tttcgagttc gggtaaaatg cggtttgcaa gctgtttgcc taactccacg ccccattggt
      361 cgaaactgta aatgttgaac agcacgcctt gcacgaagat tttgtgttca tacattgcaa
      421 ttaatgcccc taagctgtac ggggtgattt tttgcaccaa gatagagttg gtcggtttgt
      481 ttccgctgaa cactttaaac ggcacgattt cagctacttc ttccaacgtt ttacctgcat
      541 ctaaaaactc tttttcaact gtttcttttg atttaccgaa agctagtgct tcagtttgag
      601 caaagaagtt agaaagcagt ttgttgtgat gatcgcttaa cggattgtga gtttgtgccg
      661 gtgcgataaa atcacaagga attaattttg tgccttgatg aatgagttga tagaatgcgt
      721 gctgaccgtt agttcccggt tcgccccaaa taatcggacc tgtttggtga gtaaccggtt
      781 tgccgtcacg ccccacaaat ttaccgtttg attccatatt gccttgttgg aaataagccg
      841 caaagcggtg catatattgg tcgtaaggta aaatcgcttc tgattcagca cctaagaaat
      901 tgttattcca aatgccgact aaagctaacg tagttggaat gtttttctca atcggcgtga
      961 ttaagaaatg cttatccatt gcgtgagcac cagcaagtag ctgctcgaag ttctcaaaac
     1021 ctaatgaaag ggcaatcgaa agcccgatgg cagaccataa tgagtaacga ccgccaaccc
     1081 aatcccagaa ttcaaacata ttagcggtat caataccgaa tttttccact tctttggcat
     1141 tggttgaaag tgccacgaaa tgttttgcta ccgcagattc atctttggca gaatcaagta
     1201 gccatttgcg agctgaaagg gcattggtca tcgtttcttg agtggtaaag gtttttgaag
     1261 ccactaacac taacgtggtt tcaggattaa ctttacgcaa cacttcagca atatgcgtgc
     1321 cgtccacgtt agacacaaag tgcatattaa ggtgattttt ataagggcgt aatgcctctg
     1381 ttaccatata aggacctaga tccgagccac caataccgat attgatcaca tcagtaatcg
     1441 ttttgccggt atagcctttc cattcgccgg aaataacacg ctcgcagaaa tctttcattt
     1501 tcgctaacac ggcattcact tccggcatta catctttgcc atcgactgcg attggtgtat
     1561 tggcacggtt acgcagtgca acgtgcaata cggcacggtt ttcagtgcgg ttgatttttt
     1621 tgccggagaa catcgcattt tttgctgatt ccaaaccaca ttcatttgcc agttggcgaa
     1681 gtaaggttaa ggttttttcg ttgatcgcat tttttgaaaa atcgactaat aattgatctt
     1741 caaaacggag ggaatatttg tcgaaacggt ttggatcttc ggcaaaaagt tgaggaatag
     1801 aaaggttgtc tgctttatgt tgctctaagg cattccaagc aaaagtttgc acagggttaa
     1861 ttttttgcat ctgagtttcc ttatactaaa ttaaaaaatc gattattcat ctgttctttg
     1921 agtcagttca tcaagacgtt tttctaattc ggttaatttt tcacgagtgc ggagtaacac
     1981 ttgagattga acatcaaatt cttcacgagt gacaacatca agacgggcta attgggcttg
     2041 aaggatttgc ttaaattttt catctagatc attaccgaca ttttttaagc tttgcggtaa
     2101 ggcgttatgg agttgttgag caatcgcttc taagttcttc ggcgtgagca tatttaaatt
     2161 ccttactata aatgagtatc ccaattttat tgaattttgg gtttaagaga aagcaaattc
     2221 tttgtaaaat atcacctttc gcaaaataca agcaaaaaag accgcttgta ttttttattt
     2281 gtggttcgta aacctcccac acaaaaaaac taaggacaca aaatgactca tcaaaaacca
     2341 aaagccgttg tgattttttc cggcggtcaa gactctacca cttgtttatt ccaagctatt
     2401 gctgaatttg gcaaagaaaa tgtggaagtc gttacctttc aatatgggca acgccattcg
     2461 attgaacttg aaaaagcacg ctggatttct aacgatttag gcgtcaaaca aacgctgatt
     2521 gatacttcgg tgattaaagc gattactacc aatgcgttaa tggataataa tgctgaaatt
     2581 caacaacaag gcagcacacc caataccttt gtggatgggc gtaatgcctt atttctgctt
     2641 tataccgcca tttatgccaa agggcagggc attcaaacta tttttaccgg cgtgtgtgag
     2701 accgatttta gtggttaccc tgattgccga gatgtatttg taaaatcaat gaatgtaacg
     2761 ctaaatttag cgatggacta taatttcaac attcgcacgc cattgatgta tttaactaaa
     2821 aaagaaactt gggcattagc agataagctg ggtgcttttg attatattcg agaccatacc
     2881 cacacctgtt atttaggcgt tgaaggtggt tgccacgact gcccgagttg cgtattgcga
     2941 gaaaaaggct tgaatgaata tttagcagaa agggaggtaa gcaatgttta aaattgctaa
     3001 agaatttagt tttgatatgg ctcatatgct agatggacac gatggcaaat gccaaaactt
     3061 acacggacac acctacaaat tgcaagttga ggtggcaggt gagctatttc aagagggagc
     3121 taaaagagga atggtaatgg actattcgga tctaaaagca gtggtaaaac aacatatttt
     3181 agagccgatg gatcacgctt ttatttacga cttacagagt gagcgtgaaa gccaagtagc
     3241 acaattatta attgatttaa actccaaggt ttatggtatc ccaactcgta ccacagccga
     3301 agaaatggcg aaatatatgt ttgaaaaatt agagaaagtg ggcttaccgg ttagcctaat
     3361 tcgcttgtgg gaaacgccta cttcttattg tgagtacagc aaataatggc ggagttaatt
     3421 tcaaaccctc gttttccgat tgttgagatc tttgaaagct tacaagggga aggctttaat
     3481 accggaatgc cggctatttt tgtacgtttt ggcaaatgca acctcacttg cccgtggtgc
     3541 gataccaatt acaatcaatt tgagcaatgg acgttaagcg atattcttgc caaagtaaga
     3601 agctattctg cgaaaaatgt gattattacc ggaggcgagc caacgattca gcctaattta
     3661 tcgttattat tagaacaatt taaaaaagag ggatattttt tagcgatcga aaccaacggc
     3721 ttaaaagagg ttccggcaca aattgattat attgcgacca gcccaaagcg aatgtaccaa
     3781 gaaaagtatc aacgccgttg cattgaattt gcgaatgaag tccgtatcgt cgttgatgga
     3841 gaagtacagg gtttctgtga gcaaatagaa agccaaatta aagctgagca ttactatctt
     3901 tcaccttgtg aggtagaggg aaaaatgaac ttgctggaaa ccatcaccca gattgggtta
     3961 ttaaatcagc gtccaaataa gcctaaatgg cagctaagta tccaaacgca taaaattgtg
     4021 gggattgaat aagtagaagt tagaagatga tttgtaaaag atcagacttg atctgacaat
     4081 tcactctaaa aagtgagagt ttcccgttta gaatatgagt gtccaaa
//
LOCUS       DANLXQ010000185         4110 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.188, whole genome shotgun sequence.
ACCESSION   DANLXQ010000185 DANLXQ010000000
VERSION     DANLXQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.188"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..386
                     /locus_tag="PXD94_002393"
     CDS             <1..386
                     /locus_tag="PXD94_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287203.1"
                     /translation="KRISLHLKILDFDKGRNTYLAPADLFILDEHLNEFILGNGGDEI
                     EKISKNNKTQFSSTIRENQINFIRALLYMDYGIETPQEVKNAINSGELGRKIEKFRRE
                     NPNIENDKNFKFPTSVTLSNWYSKA"
     gene            464..715
                     /locus_tag="PXD94_002394"
     CDS             464..715
                     /locus_tag="PXD94_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6287204.1"
                     /translation="MQLQKTISTFTQPNHRGIYVMTYSEQSYLNVKDVCEMLQLNRST
                     IYRQIKQGIFPKPTKIGKSSRWKFADIKAFLEPKPQEAN"
     gene            715..1095
                     /locus_tag="PXD94_002395"
     CDS             715..1095
                     /locus_tag="PXD94_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6287205.1"
                     /translation="MAGLSIICPFCGNTARIRGSSRPSLLSYQAQLTCSNCGQLKADF
                     VGQLINIKRAVFIECDEANQWDKPENELIKEGKIQGKDNAQRLKELRGSQQDLFAKVP
                     GQLPPEKITPAEKIAKRANLSKTH"
     gene            1406..2467
                     /locus_tag="PXD94_002396"
     CDS             1406..2467
                     /locus_tag="PXD94_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDL6287206.1"
                     /translation="MKDLSPNQKQYTPFSAIMQKNLMDLLTKSARSPYSFSTVAKSTA
                     GRENPELHNTANSSTPLTRAIFIRSLHTPKERRNKACSSMVACSGQGSPLAVFGSPFV
                     AVFSPRYTLSPCTVRSVADSPLKLTNGLQKMNPSQITGKNRLNSFLFNNNQNDYSLLF
                     TNAKQSTPISAKISKNQDLCLTTLNRSPYASRAFAKSKAECGNSKEHQANNSTPLTRA
                     FFVRSSRTPKECQNKACSSMVACSGQGFALDCLPDVAVFHPVTRYRPTLWKMLAIAPK
                     NQHQELSKMKAFAYSFLCAIRTPETDTYQETVVRIISDSEQNARFQLTADYRLLLPRP
                     VAKIRLNSTAYTVKGGIYA"
     gene            2460..2624
                     /locus_tag="PXD94_002397"
     CDS             2460..2624
                     /locus_tag="PXD94_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287207.1"
                     /translation="MLNYSDIEQIRYFMDQAEEALDNNEEFKAVAELNTAQQFILALL
                     MNYEQGGENE"
     gene            2617..3252
                     /locus_tag="PXD94_002398"
     CDS             2617..3252
                     /locus_tag="PXD94_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287208.1"
                     /translation="MNNTTGQATQRNWQEIIANLQRLSESGESTIEIAKRYEAFSKTA
                     EQIQALVNNNGSEAEKEAFFAPVERFYLGLLETETRLKDAIAKDEENAQKLKEQKAAR
                     EAEQKRWDNLPFYAKDKFSDLICIESRETLEDKFFAISCMFDALKAVPNLLEIGLREE
                     GKDSVIDRLAEMSKLYLLFVDEQFSLLANEIQSGKYGKLDLNGSQNKGDLC"
     gene            3246..3464
                     /locus_tag="PXD94_002399"
     CDS             3246..3464
                     /locus_tag="PXD94_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287209.1"
                     /translation="MLTHRITLPDGTEIEFFAETDKETFAEIQRDLEGLKLPTFAEFV
                     AQERKKHRAKQAVKKARKIAKLARRKNR"
     gene            3606..4091
                     /locus_tag="PXD94_002400"
     CDS             3606..4091
                     /locus_tag="PXD94_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287210.1"
                     /translation="METALQLAQHLTGWTEEENSQAILKNFLDWKETFGEKTREETKI
                     VETLLDWINENEASFIEYPFNQEQRTPPKVAGYRILANNSTKTNEYFFIYPKQFKETL
                     AEFPLLMACTKLHEAGILHKPPRPEKGYEFQYTIAPKITGGSKKRAYKIVPIALEEEN
                     E"
BASE COUNT         1492 a          854 c          788 g          976 t
ORIGIN      
        1 aaaaaagaat aagtttacat ctaaaaatac tggattttga caaaggcaga aatacttatt
       61 tagcacctgc agatttattt attcttgatg aacatctaaa tgagttcata ttaggtaacg
      121 gcggagatga aatagaaaaa atatcaaaga ataacaaaac tcaattcagc tcaacaatac
      181 gagaaaacca aatcaatttt attagagcct tgctttatat ggattatggg atagaaacgc
      241 ctcaagaagt aaaaaacgcc attaattcag gggaactagg cagaaaaata gagaaattta
      301 ggagagaaaa ccccaatata gagaacgata aaaatttcaa attccctaca agtgtaaccc
      361 ttagcaactg gtattcaaaa gcctaaaata ttggaaattt ccgaatctac tttggaaatt
      421 tccaaacttc taaaacctac tcacctcaca atctccccca acaatgcaac tacaaaagac
      481 aataagcaca ttcacgcaac caaaccacag ggggatttat gtaatgacct attcagaaca
      541 aagctattta aacgtgaaag atgtatgcga aatgctacaa ctcaaccgct caactattta
      601 tcgacaaatt aagcaaggga ttttccctaa gccaactaag atcggaaaat caagccggtg
      661 gaaatttgcc gacatcaaag catttttaga gccaaaacca caggaggcga actaatggca
      721 gggctttcta ttatttgccc gttttgtggc aataccgcac gaattagagg cagttctcgc
      781 cctagccttt taagttatca agcacagcta acttgttcaa attgcggaca attaaaagcc
      841 gactttgtag ggcagctaat caacatcaaa cgggccgtat ttattgagtg tgatgaagca
      901 aatcaatggg ataaacccga aaatgaacta atcaaggagg gaaaaattca aggcaaagac
      961 aatgctcaac gcctcaaaga actaagaggc tcacagcaag atttatttgc aaaggtgccg
     1021 gggcaactac cgcccgagaa gatcactccg gcggagaaaa tcgccaagcg ggcaaacctt
     1081 tcaaaaacgc attaaacagg gggataaatg gcaacaaaaa agccccgcaa ggcaaaccat
     1141 acggagctat taaaatcgca taatgcgaaa gatatgcaaa gaaattaaag caggcttgac
     1201 gatttctgaa ggtgttagat tctttctaaa gtctttaggg ataggtttat ttctagcatt
     1261 gtttttttat tttttgccgt atttagcaga atttataatc gccgtaaaaa aacaactagc
     1321 aaaaaaatcg gcttactttc aatgataaaa gcaagccgaa atgacaatcc aaatttttaa
     1381 ctttaaataa agagtacaag aagaaatgaa agatttatct ccaaatcaga aacaatatac
     1441 gccattttcg gctataatgc aaaaaaattt aatggattta ttgacaaaat ccgcccgttc
     1501 gccttattct ttctctaccg ttgcaaaatc aacggcaggg cgtgagaacc ctgaactaca
     1561 taatacggcg aacagtagca cgcctttaac ccgtgctatt tttattcgta gcttacacac
     1621 acctaaagaa cgccgaaata aggcgtgttc gtctatggta gcgtgtagcg ggcaaggttc
     1681 gcccttggct gtttttggtt cgccgtttgt agcagttttc tcaccccgtt acacgctatc
     1741 gccctgcacc gtgagaagtg tagccgatag tcctttaaaa ttaacaaacg gactacaaaa
     1801 aatgaaccct agccaaatta caggcaaaaa ccgcctaaac tcttttctat ttaacaataa
     1861 tcaaaatgat tattcgctat tatttactaa tgcaaaacaa tcaaccccga tttctgctaa
     1921 aataagcaaa aatcaagatt tatgcttgac aaccctcaat cgttcgcctt atgctagccg
     1981 tgcttttgca aaatcaaaag ccgagtgtgg aaactcgaaa gaacatcagg cgaacaatag
     2041 cacgccttta actcgtgctt tttttgttcg tagctcacgc acacccaaag aatgccaaaa
     2101 taaggcgtgt tcgtctatgg tagcgtgtag cgggcaaggt ttcgcccttg actgcttacc
     2161 tgatgttgca gttttccacc ccgttacacg ctatcgccct acattgtgga aaatgttagc
     2221 gatagcccct aaaaatcaac atcaggagct atcaaaaatg aaagcattcg cttattcatt
     2281 cctttgtgct attcgcacac ccgaaacaga tacttatcaa gaaaccgtag tacgcattat
     2341 ttcagacagc gaacaaaacg cccgttttca gcttacagcc gattatcgct tactactccc
     2401 ccgccctgtt gcaaaaatcc gcctaaattc taccgcttac acggtgaaag gggggattta
     2461 tgcttaatta ttccgatatt gaacaaatcc gctattttat ggatcaagcc gaagaggctt
     2521 tagacaataa tgaagagttc aaagccgtag cagaacttaa cacagcacaa caatttattc
     2581 tggctttatt aatgaactac gaacaagggg gtgaaaatga ataatacaac aggacaagct
     2641 acgcaaagaa actggcaaga aattatcgct aatttgcaga gactcagcga atccggggaa
     2701 agcacaatcg aaatcgctaa acgctatgag gcattttcta aaaccgccga gcaaatccaa
     2761 gccctagtga ataacaacgg cagcgaagcc gaaaaagagg cgttttttgc ccctgttgag
     2821 cgtttttatc ttggcttact agaaaccgaa acacgcttaa aagatgccat agccaaagac
     2881 gaagaaaacg cccaaaaatt gaaagagcaa aaagcagcta gagaggcaga gcaaaaaaga
     2941 tgggacaacc tacctttcta cgccaaagac aaattcagtg atttaatttg cattgaaagc
     3001 cgagaaacgc ttgaagataa attctttgcg atctcttgta tgttcgatgc cctaaaagcc
     3061 gtacctaatc tattagaaat aggcttgcgt gaagaaggca aagacagtgt tatagaccga
     3121 ttagccgaaa tgagcaaact ctatttactg tttgtagatg agcaattttc actactggca
     3181 aacgaaattc aaagcggtaa atatggcaag ttagacctaa acggctcaca gaataagggg
     3241 gatttatgct aacgcaccgc attacactcc ctgacggcac agaaattgaa ttttttgcag
     3301 aaaccgacaa agaaacattt gcagaaattc aaagggactt agagggcttg aaattgccaa
     3361 cctttgccga gtttgttgcc caagagcgga aaaaacaccg agcaaaacaa gcggtcaaaa
     3421 aagcccgaaa aattgcaaaa ttagcaagac ggaaaaaccg ctagcaccta tcaaaaagac
     3481 ttattattgc agaaaatcac gctttaataa aaacgaccta taaagcaatc cgcaagaaat
     3541 ggttaagttt aactgaaaat atgagcggac aggttcagcg ggtagcgtgc gatagattcg
     3601 caataatgga aaccgccttg cagttagccc aacatttaac aggctggaca gaggaagaga
     3661 acagccaagc gattttaaaa aacttcctag actggaaaga aactttcggc gaaaaaacaa
     3721 gagaagaaac taaaatagta gaaaccttgc tagattggat aaatgaaaat gaggcgagtt
     3781 ttatcgagta cccattcaac caagagcaaa gaacaccgcc taaagttgcc ggctatagaa
     3841 tacttgcgaa taacagcact aaaacaaatg agtacttttt catttaccct aagcaattta
     3901 aagaaacgct ggcagaattt ccgctattga tggcttgcac caagttacac gaggcaggga
     3961 ttttacataa gccaccacgc ccagaaaaag gctatgaatt tcagtacaca atagccccta
     4021 aaataacagg agggagcaaa aaaagagctt ataaaatcgt accgatagca ttggaagaag
     4081 aaaacgagta attacagcaa aagcaagagt
//
LOCUS       DANLXQ010000186         4057 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.189, whole genome shotgun sequence.
ACCESSION   DANLXQ010000186 DANLXQ010000000
VERSION     DANLXQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.189"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(441..1535)
                     /locus_tag="PXD94_002401"
     CDS             complement(441..1535)
                     /locus_tag="PXD94_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL6287211.1"
                     /translation="MKKTTLAILASALIANSALAITIYDHKETGTQIEFIGSARIKWE
                     STSDKTSPVNGKATRNHVNHAVDNNGSRFGFRLTQQVGHGFYALGRVEWRMRGDSSSQ
                     HNFDHIYDHQLYAGIGHKQYGELTYGNMTNIADEVKQTDLPNTLSLSDGLLTGSSRRT
                     LQYVYKGIEGLKLGAFYGGNSQRGNNGLDLVNKRKDIWGAAAIYNYKIDDNQSLKLAT
                     GATRERSEQSIGNYERIALAFGTAYTYGKTTLGLDLERRETEDQNAIGHKRIEKEVRT
                     VLLHRLTDQWNAYTMYAYKENKLNAVGLANDSKVKRNQFMLGTEYYIVPKHLKGFVEW
                     QATRAKHYTNDVKTAKSRNYTTVVGLRAYW"
     gene            1669..2847
                     /gene="lpxB"
                     /locus_tag="PXD94_002402"
     CDS             1669..2847
                     /gene="lpxB"
                     /locus_tag="PXD94_002402"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL6287212.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVAGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLVKTDYISLPNLLAQAPLVPELIQEACNPE
                     NLAQELSLFLADDEKNQQHKALLKQQFMQLHQSIQCNADEQAAQAVIDLLNGSTSGQI
                     "
     gene            2874..3926
                     /gene="sohB"
                     /locus_tag="PXD94_002403"
     CDS             2874..3926
                     /gene="sohB"
                     /locus_tag="PXD94_002403"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL6287213.1"
                     /translation="MWKEVLLNYGIFLLELLTVFGIIAVVVMLILESKKQNENGSISI
                     TNLSEKYKEQQKSLSGFFLSEEELKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSR
                     LFVLNFNGDMMAHEVNSLRREIDAVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQ
                     RLRDRNIYLTAAVDKVAASGGYMMACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKH
                     DIDVDVMTAGEYKRTVTLVGENTEKGKQKFQQELEETHSLFKQFVARHRPQLDIEQVA
                     TGEHWFGQQAIELNLVDEIATSDDLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSA
                     ENLLAKILHKSRSTLM"
BASE COUNT         1210 a          698 c          884 g         1265 t
ORIGIN      
        1 tgctgtggca gaacaccgat ggcaacctta cttgatggaa aacggatttg ggtggaaaag
       61 aatttaagct caaattaatc tgacagacac ggtaatttaa aacgggggac tgtcagatta
      121 ggtttgatct tctacatata ataacccctc atcacttttt gcagtttaac ctcttttgca
      181 acagataatt taaatttagg cgttttggct gctcagttat tgccattatg attatattag
      241 ttcacactaa aattttagtg ttgtttctta aagctgaaga tgctaatagg tgagtaagcg
      301 gtttttacca ctttgaagat aaatgcaata gcttcttgtt tgaaggatta gttgtattta
      361 atcataaaaa atctgcacct taatcagttg gttagcccaa cttttaggtg cagatcattc
      421 tagtacgctt ttcagtttta ttaccaatat gctcgtaagc ctacaacagt cgtataattg
      481 cgagattttg cggtttttac atcattagta tagtgtttag cacgagttgc ttgccattca
      541 acaaagcctt ttaagtgttt tggaacaatg taatattccg ttcccaacat aaattggttt
      601 cttttaactt tgctatcatt tgccaagcct acagcattaa gtttgttttc tttataagca
      661 tacattgtgt aagcattcca ttgatcggtt aaacgatgga gtaacacagt gcgaacttct
      721 ttttcgatac gtttgtgacc gatagcattt tgatcttccg tttcacggcg ttctaaatct
      781 aagccaaggg tagtttttcc gtaggtataa gctgtaccga aggcaagagc aatgcgttca
      841 taattgccga tagattgctc agaacgctca cgggttgcac ctgttgctaa ttttaagctt
      901 tggttatcat caattttata gttataaatt gctgctgcac cccaaatatc tttacgttta
      961 ttgactaaat ctaagccatt gtttccacgt tggctatttc cgccatagaa tgctccgagt
     1021 tttaatcctt cgatgccttt gtaaacatat tgaagcgtac gtcgtgatga accggtaagt
     1081 aagccatcac ttaagctgag tgtatttggt aagtcagttt gttttacttc atcggcaata
     1141 ttagtcatat tgccgtatgt taattcaccg tattgtttat gcccgattcc tgcatagagt
     1201 tgatgatcat aaatatgatc gaagttatgt tgtgagcttg aatcgccacg catacgccat
     1261 tccacacgac ctaaagcata gaatccgtga cccacttgct gggttaaacg gaaaccaaaa
     1321 cgagagccgt tgttatccac tgcgtgatta acgtggttgc gggtagcttt accatttacc
     1381 ggtgaggttt tatctgaggt gctttcccac ttaatacgag cagaaccaat aaattcaatt
     1441 tgtgtaccgg tttctttatg atcgtaaatc gtaatagcta gagctgagtt tgcgattaag
     1501 gctgatgcta aaatcgctaa tgttgttttt ttcataaaaa tcactccata tgaatagata
     1561 aagggaggta gtgtatgtga atattcattg ctagacaata atgttttgtt agtttatatg
     1621 atgattggtt ataatagagt cacttacaga tcttcaataa tatttaatat gaaacaacaa
     1681 ttccccgtta ttggcttagt tgctggtgag gtctctggcg atattcttgg tgccgggtta
     1741 attaaggcgt taaaagtaca ttacccgaat gctcgattta tcggtgttgc aggctctcga
     1801 atgattgagg ctggctgcca gtctttgttt gatatggaag agctttctgt aatggggcta
     1861 gcagaggtgg ttaaacattt acctcgttta cttaagcgtc gtaaacaggt tattgatgag
     1921 atgcttaaat tgaaaccaga tgtatttatc ggtattgatg caccggattt taatttaacc
     1981 gttgaagaaa aactcaaagc gagtgggatt aaaacaatcc attatgttag cccttcggtg
     2041 tgggcttggc gacaaaagcg ggtgtttaaa attgccagag cagctaattt agtgttggca
     2101 tttctgccat ttgagaaagc gttctatgat aaattcaatg tgccttgtcg ttttatcggg
     2161 catacgatgg cagatgcgat cccactcgaa ccaaatcgta cagaggcatg tttagcgtta
     2221 aatattgatg aatccaagcg atatatggca attttggtgg gaagccgtgg cagtgaaatt
     2281 cagtttttgg ctgagccttt tttgaaggct gctcagttgt taaaagcccg ttttccggat
     2341 ttacagtttt tagtgccaat ggtcagccaa aagcgaatgg agcaatttaa tgcaattaaa
     2401 cagcaagtag ctcccgagtt ggaactgaat attattcaag gcaaggctcg tcaggctatg
     2461 attgcgtcag aatgtactct tttggcatcg ggaacagcag cactagaggc aatgttgtgc
     2521 aaatctccga tggtagttgg ttatagaatg aaaccgctga cttattggtt agcaaaaaaa
     2581 ttagtgaaaa ccgattatat ctccttaccg aatttattgg ctcaagcccc attggtacca
     2641 gaattgatcc aagaagcatg caacccggaa aatttagctc aagagttatc tctattttta
     2701 gctgatgatg agaaaaacca acaacataaa gcattgctaa aacagcagtt tatgcagcta
     2761 catcaatcaa ttcaatgtaa tgctgatgag caagctgccc aagcggttat cgatctccta
     2821 aacggctcga caagcggtca aatttagtta gttttttgta aaaaggaaaa tttatgtgga
     2881 aagaagtatt acttaattat ggtatttttt tattagagtt attaactgta tttggcatta
     2941 ttgcagtggt ggtgatgctg attttggaat cgaaaaagca gaatgaaaac ggctcaattt
     3001 caattaccaa tttatctgag aaatataaag agcagcaaaa atctctttcc ggcttttttc
     3061 taagtgagga agaattaaaa catcaggaaa agcaggagaa aaaagtagca aaagagaaag
     3121 ctaaagcaga aaaaaaacgc ttaaaagcag gcgaagaatg tgtagaaagc ccttcccgtt
     3181 tatttgtatt gaattttaat ggcgatatga tggctcacga agtaaattcc ctacgccgtg
     3241 aaattgatgc ggtaattagc ctcgcgaatc cggaaaaaga tgaagtttta cttaaacttg
     3301 aaagtccggg cggagtggtg cacggttatg gattggctgc ttcgcagtta caacgtttgc
     3361 gtgatcgcaa tatttattta accgcagcgg tagataaagt agctgccagc ggtggttata
     3421 tgatggcgtg tgtggcgaat aaaattgtct ctgccccatt tgcggtgatt ggctcagtgg
     3481 gggtggtggc tcaggtgccg aatatccacc gcttactgaa aaaacacgat attgatgtgg
     3541 atgtgatgac tgcaggtgaa tataaacgca cggtgacgct ggtgggagaa aataccgaaa
     3601 aagggaaaca gaaattccag caagaactgg aagaaacgca ctctctgttt aagcaatttg
     3661 tggctcgaca ccgcccacaa ctggatattg agcaggttgc aacaggcgag cattggtttg
     3721 gtcagcaagc gattgagcta aatttggttg atgagattgc aaccagtgat gatctgctcc
     3781 tcaatgcaat taaggaaaaa gaagtgatcg agctaaaata taaagagaag aaaaaacttg
     3841 ctcagaaatt gggttctcaa ttcgagcaat cagcagagaa cctgctcgct aagattttac
     3901 ataaaagtcg ttcaacgctg atgtagcgta tatattatta aagggctatc gtttatatag
     3961 ccccttattt tatcagttag taatagaatg aagtatttgt tcactattta aatgacctgg
     4021 aatacttttc tttaaattga gtgaagaatc taacaat
//
LOCUS       DANLXQ010000187         4014 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.190, whole genome shotgun sequence.
ACCESSION   DANLXQ010000187 DANLXQ010000000
VERSION     DANLXQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.190"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            150..455
                     /gene="cyaY"
                     /locus_tag="PXD94_002404"
     CDS             150..455
                     /gene="cyaY"
                     /locus_tag="PXD94_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL6287214.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
     gene            564..2363
                     /gene="recQ"
                     /locus_tag="PXD94_002405"
     CDS             564..2363
                     /gene="recQ"
                     /locus_tag="PXD94_002405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL6287215.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRKKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPSEAVLFYDP
                     SDYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLNYFGEHRQEQCKNC
                     DICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNLKIREHQHDQLSVY
                     GIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLRGEKQLQLALPRLG
                     ISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDATLQEMAEFLPTSK
                     VEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
     gene            2522..3064
                     /gene="infC"
                     /locus_tag="PXD94_002406"
     CDS             2522..3064
                     /gene="infC"
                     /locus_tag="PXD94_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL6287216.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            3328..3525
                     /gene="rpmI"
                     /locus_tag="PXD94_002407"
     CDS             3328..3525
                     /gene="rpmI"
                     /locus_tag="PXD94_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL6287217.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            3634..3987
                     /gene="rplT"
                     /locus_tag="PXD94_002408"
     CDS             3634..3987
                     /gene="rplT"
                     /locus_tag="PXD94_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL6287218.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
BASE COUNT         1331 a          789 c          797 g         1097 t
ORIGIN      
        1 taataatgag gataaaataa aaaatcaagc taaacttaaa aagcctaatt atttcttaac
       61 aaaaatacaa aaagaacttt acagaccatt taaacagcta aaacaaaaag tatttactac
      121 ttatataaca ttcaaataag gatacaccaa tgaacacagc acaattccac caagccgtag
      181 agcaagtatg gcaacaaatt gaagaaaaaa tttatgacga aggattatca gtcgatactg
      241 aaatccaagg agcggtctgc accttaacct ttgatgatga atcgcaagtt atcattaata
      301 aacaagaggc aatgctggaa ttatggcttg ccagcaaatt gggtggtttc cactttaaaa
      361 atgtagaagg cgaatgggta acagatgaag gtcgctcgtt ctggcaacat ttagaagaag
      421 cttttgctcg acacggagaa agcgtttctt tctaatgcct aattgttaac taagacagca
      481 ctcgtctttg atgagtgctt tattttcatt ttaaaagata ctctcagaag acgagtacta
      541 tcataagtag caaccaacta ccaatgaaca ccaccgcaat ttctgtttta aataatattt
      601 ttggctatca aaaattccga gacgggcaag aagaagttat caatgccgtg ttggaaaatc
      661 gtgattgcct cgtgattatg acaacaggcg gtggcaaatc cctttgctac caagtgccgg
      721 ctttgtgcct agacggcatc actttggtga tttcgccgct tatttcgttg atgaaagacc
      781 aggtggatca acttatcacc aacggcattg aagcagctta tcttaactcc actcaaacgc
      841 tcgaagagca gcaacttgtg gaacaaaaag ccttatccgg tcaattaaaa ttactctatc
      901 tttcccctga aaaggtaatg actcaaggct ttttccactt tatttcgcta tgtaaaatca
      961 gctttattgc ggttgatgaa gcccattgtg tttcccaatg ggggcacgat ttccgccctg
     1021 aatataccct gctaggtggc ttacgaaaca ctttcccgaa tgtgccgttg atggcactca
     1081 ccgccacggc agatctaaca acccgctccg atattctgca tcatttacgc ttaaatagcc
     1141 cacatatcta tttaggcagc tttgatcgcc ccaatattcg ctacaccgtg caggaaaaat
     1201 ttaaaccgct tgaacagctc attaaattca tcagcaaaca gcaaggcaaa agcggtattg
     1261 tttactgcaa cagtcgcaaa aaagtggaag aaatcaccga gaaactctca gcccgtaaaa
     1321 tcagcgtgat gggctatcac gccggtatga gttttgaaca gagagaacga gtgcagaatg
     1381 cctttcagcg tgatgatatt caaattgtgg ttgccactat tgcgtttggt atggggatca
     1441 acaaatccaa cgtacgtttt gtggtacatt tcgatttacc tcgctctatc gaatcttact
     1501 atcaagaaac cggacgagcc gggcgagatg atctcccttc tgaagccgtg cttttttatg
     1561 atcctagcga ttatgcttgg ttacaaaaaa tattattgga agagccggaa agcgaacaga
     1621 gaaatattaa acagcataaa ttacaggcaa ttggcgattt cgccgaatca cagacttgcc
     1681 gccgtttagt cttactcaac tactttgggg aacatcgcca agagcaatgc aaaaactgcg
     1741 atatttgttt agatccaccg caacaatacg atggcttact tgatgcccaa aaagtgatgt
     1801 cggtgattta ccgcaccggt caaattttcg gtcagcacca tattattgcg gtattgcgag
     1861 gaatgaacaa tctcaaaatt cgtgaacatc aacacgatca actttccgtt tatggtatcg
     1921 gaaaagaaca aagccaacat tattggcaaa atgtggtgcg gcaactgatt catttagggt
     1981 tgattattca aaatattgct aaccattccg ctctgcaatt aaccgaaagt gccagaccat
     2041 ttttgcgtgg agaaaagcaa cttcagcttg cattgccacg tttaggtata tctgcgactg
     2101 catttgtgca aaaacaagca agtcatcgct atgacaaaga cctattcgca cgcttacgtt
     2161 ttttacgcaa gcagatagct gacctcgaaa atattccacc ttatatcgtc tttaacgatg
     2221 ccaccttgca agaaatggca gaatttctcc cgacttcaaa agtggaaatg ttagaaatta
     2281 atggtgttgg tgaacgaaaa tttgaacgat ttgggcaagc atttatgagt ttaatcaaag
     2341 aatatcaaaa taaccgaaaa taggcagtta aataagggct ttattattgc ataagcactg
     2401 aaaatcttat agaatccgca cgtttagctg ttttttaatc acttaaaaca agcaatctaa
     2461 aatatttata cgctctcaag cggtaaaatt taagcaaaaa tttactagaa ggaataaggc
     2521 tattaaacct gtaaaacgtg ttcaatcaaa tcgtgcacat cgtcttaatg acgaaattcg
     2581 tggcgtaaaa gaagttcgct taaccgatga aaacggcgaa caagttggta ttgtatcact
     2641 tcaagatgct ctaaatagag cagaagaagc tgaattggat ttagtggaaa tcagcccaaa
     2701 tgctgaacca ccagtttgtc gtattatgaa ctatggtaag ttcatctacg aaaaagagaa
     2761 agcagcaaaa gaacaaaaga aaaaacaaaa agttgttcaa gttaaagaga tcaaattccg
     2821 tccgggaact gatgatggcg attatcaagt taaacttcgt agcattatcc gtttcttaga
     2881 agatggggat aaagtaaaaa tcacagtgcg tttccgtggt cgtgaaatgg ctcaccaaga
     2941 aatcggtatg gacgttttag aacgtgtcaa agcagatact gcagaaattg caatggtaga
     3001 gtcagcccca ggcaaattgg aaggtcgcca agttgtgatg gtgatcgcac ctaagaagaa
     3061 ataatttcga taagcacatt gttttaatgt gcttttttct tgataaagct taaattggct
     3121 tgcatagcgg ttatattttc gctaaaattc gcaagctttt ttgcctagcc aagaaagaga
     3181 aaatgctcaa caggcaaact cagcagcctg cttgactttt taacgatgaa aatcgtatag
     3241 cggagaataa gtgcttccaa gtaattttgt tcacaaaatt cgccttattt tcataatatc
     3301 taacaaacaa tgcggagttt tttaacaatg cctaaaatta aaacagtacg tggtgctgct
     3361 aagcgtttca aaaaaacagc ttccggcggt tttaaacgta aacaatctca cttacgtcac
     3421 attttgacta agaaaaccac taaacgtaaa cgtcaattac gtcacaaatc aatggtagcg
     3481 aaagctgacc aagttttagt agtagcgtgc ttaccatacg cataagcctt ttaggttagc
     3541 gtagtgtagt aaagtacgga cgtacggata gaattcttag taaaggtcgt gatgacgacc
     3601 accaatcaaa atagacaatt aggagattaa ataatggctc gtgtaaaacg tggtgttatt
     3661 gcaagagcac gccataagaa agttcttaag gctgctaaag gttattatgg tgcacgttca
     3721 cgcgtgtatc gcgttgcttt ccaagcagta gttaaagctg ctcaatatgc ttaccgtgac
     3781 cgccgtcaac gtaaacgtca attccgtcaa ttatggattg ctcgtattaa cgcagcagct
     3841 cgtcaaaacg gtttatctta cagcaaattc atcaacggct tgaaaaaagc atctgttgaa
     3901 atcgaccgta agatcttagc tgacatcgca gtatttgaca aagttgcttt cgcagcttta
     3961 gttgaaaaag caaaatctgc tctttaattt taaagaatag aaatgaggac gcct
//
LOCUS       DANLXQ010000188         3999 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.191, whole genome shotgun sequence.
ACCESSION   DANLXQ010000188 DANLXQ010000000
VERSION     DANLXQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.191"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            46..1521
                     /gene="mepM"
                     /locus_tag="PXD94_002409"
     CDS             46..1521
                     /gene="mepM"
                     /locus_tag="PXD94_002409"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL6287219.1"
                     /translation="MAVFLLAVFSLFVDIVLALKDQSSINNTSSQEDIIAIDKQEKYQ
                     SINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDEIVDDQTATSYEDELVGQD
                     DEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRIQNQFSYTVARGDKLKDVL
                     EQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELEYMNWLVSEKEERVYERKE
                     NGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDRQINQLAVGLQSQIATNKL
                     KKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAIQADNGRYYSRYGETLSGG
                     FARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPIGTPIIAPSDGVVEHVAYQ
                     ARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIALSGNTGGSTGPHLHYEFH
                     INGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLKM"
     gene            1531..2214
                     /gene="dsbC"
                     /locus_tag="PXD94_002410"
     CDS             1531..2214
                     /gene="dsbC"
                     /locus_tag="PXD94_002410"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL6287220.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDITCHYCHLLHTKIKEYNDLGITIRYLAFPRGGMNTQTAKQMEAIWT
                     SADKVKALNDAEDGKLPTQIKTPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEP
                     KELAKMLSE"
     gene            2299..>3999
                     /gene="recJ"
                     /locus_tag="PXD94_002411"
     CDS             2299..>3999
                     /gene="recJ"
                     /locus_tag="PXD94_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL6287221.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNALEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEKSLQLK
                     V"
BASE COUNT         1338 a          643 c          880 g         1138 t
ORIGIN      
        1 gtaattttag cgagagatcg taagcgacat aaaaaacatt ttaagattgc ggttttctta
       61 ttagcggtat ttagtctatt tgttgatatt gttctcgcac taaaagatca atcctcaatc
      121 aataatacat cttcacagga agatattatt gccattgata aacaggaaaa atatcagagt
      181 atcaacacta atttggcgga aaataatact ctccatcagg agcaacattc cagttttatt
      241 aattatgtga ctgatttact gactcagtcg gcattagtaa tcgaacatga cgacgaaatt
      301 gttgatgatc aaacagcgac ttcttatgaa gatgaattag ttgggcaaga cgatgaagta
      361 gaagaaggga ttgaaacggt tgaaaatcaa gttcctgggg aaaaactccc agaagaagct
      421 gaaaaggcat tagaagattt acttgatgtg gctgatcaag cactacgaat tcaaaaccaa
      481 ttcagttata ccgtagctcg aggtgataaa cttaaagatg tattagagca atcaggctta
      541 actgcatccg ttgcgaaaaa tttaattaag caacattcgg cattagcaca attagaaccg
      601 ggtcaacaat tttattgggt attagataat gacggtgaat tagaatatat gaactggctg
      661 gtttccgaaa aagaggaacg ggtttatgag cgaaaagaaa atggtaaatt ttcttttcaa
      721 agatttgaga aaaaagggga atggaagtta gatgttgtaa gaggaactat tcagaatagt
      781 tttgcggtta gcttaaaagc cgttggctta tcagaccgcc aaattaacca gctagcggtt
      841 ggattgcagt cacaaattgc gacgaataaa ttaaaaaaag gggataggtt tgcaattctg
      901 attaagcggg aatatattga cggcaaagta acggatttag gaaatgtgga aggaatccat
      961 gtaattagca gtaaaaaaag ttattatgct attcaagcag ataatggacg ctattatagt
     1021 cgatatggtg aaacattaag tggaggattt gctcgtcatc cattattatt tgcagcaaga
     1081 gtaacttctt ctttcaaccc caaacgcaga caccctatta cagggagaat tagtccgcat
     1141 aaaggggttg actatggcgt gccaataggt actccgatca tcgcaccaag tgatggtgtc
     1201 gtagagcatg tggcatatca agcccgtgga gcaggacgtt atattcggat aaaacacgga
     1261 catattacga ctgtttatat gcacctcagc aaaacgttgg taaaaccagg gcagagtgtt
     1321 aaaaaagggg agcggatagc attatcgggt aatacaggcg gttcaaccgg gcctcattta
     1381 cactatgaat tccatattaa tggtcgaccg gtcaacccga ttacggtaaa attgcctggc
     1441 tcaaattcag gtatgggtaa taaagaacgt caagcattct taaataaagc gaagaatgta
     1501 gaaagtagat taaaaatgta aggattctca atgaaaaaaa atctaatcac tttaggatta
     1561 atgtgtttat caaccatcac gtttgcttca gatgcaaaac taaaaagcca gcttgaaaaa
     1621 attggagcca cagatgttaa aatttcagat tctccattaa caggctttaa aatggcgact
     1681 tcaaatgaag gtgttgtata tatcagccaa gacggtcgtt atgtaattca aggtaaagta
     1741 tttgagctaa aaaatggtaa agcgattgat attactaatc aaggattgtt ggcagaattg
     1801 aattcataca ctaatgaaat gatcgtatat ccagcgaaaa atgagaaaca tgtgattacg
     1861 gtgtttatgg atattacttg ccattattgc catttactac atactaaaat caaagaatat
     1921 aatgatttag gtattaccat tcgctatttg gcattcccgc gtggtggaat gaatacccaa
     1981 acagcaaaac aaatggaagc tatttggaca tcagcagaca aagtaaaagc actgaatgat
     2041 gctgaagatg gtaaattacc aacccaaata aaaacgccgg atattgtgaa aaaacattac
     2101 caattaggga ttaaatttgg tgtgacaggt accccaaata tggtaacttc agaaggtgag
     2161 ttgattggtg gttatgtaga gcctaaagaa ttagcgaaaa tgttaagtga gtaattcatt
     2221 aacttgaata caagcggtct ttttttgtaa aatttttgca aaagaagacc gcttgttgtt
     2281 tcattaaaga agagtattgt ggaaaaaata attaaaaggc gcccattatt aacaacggaa
     2341 atagtctcta caaacccgtt attaaatcga atctatcaaa gtcgaggcat aaagaatacg
     2401 caagaactag agcataattt aaagaatttg catagaccac agcaacttgc caatattgag
     2461 caagccgttg acttgctatt acaagccttt cgtgaaaaaa gccggatcat tattgtgggt
     2521 gattttgatg cagatggtgc aaccagtaca tcgcttgccc ttattgcgtt acgccaattg
     2581 ggtttcgata aggtagatta tttaattccg gatcgttttt ctcaggggta cggattgagt
     2641 gtagcggttg ctgaaatggt attagcaaaa ggggcggatt tagtcttaac ggtagataat
     2701 ggaatttctt cgactgaagg cattgctctg ctaaaaagcc atcagattca agttttggtt
     2761 accgatcatc atttaccgcc agagagccta cccaatgcag atgcactggt aaaccccaat
     2821 ttagcttttt gtgaatttcc gtctaaatct ttagccgggg taggcgtgat cttctatgta
     2881 atgatggcat tgcgttctcg tatgagagaa caacatttat ttgaaaataa accagagcca
     2941 aattttgctg aattactgga tttagtcgct ttaggcacag tggctgatgt agtgccgtta
     3001 gatcaaaata atcggatatt ggtgcatcaa gggttacagc gaatccgttc agggcattgc
     3061 cgtttaggga taaaagcttt agcggaggtg ggcaagcgag atctttctac tttgcaagca
     3121 gcagatttgg gttttgctat cgcaccacgt ttgaatgcag ctggtcggtt agaaaatatg
     3181 tctttagggg ttgagttact aatttgtgaa aatatggaat tagctcgccg gcttgcgctt
     3241 gatcttgatt ctcttaatca aacccgaaaa gagtttgaac aagaaatgaa agcagaagca
     3301 ctgtcgattt gtgcgaattt gccaagtctt gcgcagtctg aacaggcaca cggaatagtg
     3361 ttatatcaag cagattggca ccaaggtgta attgggatct tagcttctcg cattaaagat
     3421 caatttaacc gtcctgtcat tgcttttgca caagaaagtg aggaaagtga atatctaaaa
     3481 ggttctgctc gctcgatcaa cggagtacat atgcgagatt tactggaaca tatcgatatg
     3541 cgacatccag acttgattga aaaatttggt ggacacgcaa tggcggctgg tttgactatc
     3601 catcaatcaa aattagattt atttaaacag atatttgacc aatctattaa tgcaattatc
     3661 gagcctgagc aattacaagg catcatttat accgatggtg agttaaatgc actagagttt
     3721 actttagcca atgccgagat gattaggcaa ggtgggcctt ggggacaaca ttttccagaa
     3781 cctagttttg aaggggagtt tagcattctc caacaaaaat tactcgcagg taaacattta
     3841 aaattaatgt tgcaatcaga gaaagggcaa ctatttgatg caatttggtt taatgtggat
     3901 gtaagggcgt ttcctgactt atcgatcaaa aaggcaagat tagtttatag gttggatatt
     3961 aatgaatttc gaggagagaa atctttgcaa ttaaaagtg
//
LOCUS       DANLXQ010000189         3989 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.192, whole genome shotgun sequence.
ACCESSION   DANLXQ010000189 DANLXQ010000000
VERSION     DANLXQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.192"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(25..1564)
                     /locus_tag="PXD94_002412"
     rRNA            complement(25..1564)
                     /locus_tag="PXD94_002412"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1918..3879)
                     /gene="recD"
                     /locus_tag="PXD94_002413"
     CDS             complement(1918..3879)
                     /gene="recD"
                     /locus_tag="PXD94_002413"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL6287222.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGKFS"
BASE COUNT         1049 a          998 c          739 g         1203 t
ORIGIN      
        1 acttgctgtc gttctcgatt ttggtaagga ggtgatccaa ccgcaggttc ccctacggtt
       61 accttgttac gacttcaccc cagtcatgaa tcataccgtg gtaaacgccc ccctttcggt
      121 taagctatct acttctggta caacccactc ccatggtgtg acgggcggtg tgtacaaggc
      181 ccgggaacgt attcaccgca acattctgat ttgcgattac tagcgattcc gacttcatgg
      241 agtcgagttg cagactccaa tccggactta gacgtacttt ctgagattcg ctccccatcg
      301 ctggctcgcc gccctctgta tacgccattg tagcacgtgt gtagccctac tcgtaagggc
      361 catgatgact tgacgtcatc cccaccttcc tccggtttat caccggcagt ctcctttgag
      421 ttcccgacct aatcgctggc aacaaaggat aagggttgcg ctcgttgcgg gacttaaccc
      481 aacatttcac aacacgagct gacgacagcc atgcagcacc tgtctcatgg ttcccgaagg
      541 cactctcata tctctacaag attccatgga tgtcaagagt aggtaaggtt cttcgcgttg
      601 catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc atttgagttt
      661 taaccttgcg gccgtactcc ccaggcggtc gatttatcac gttagctacg ggcaccagag
      721 ttaaacccca atccccaaat cgacagcgtt tacggcgtgg actaccaggg tatctaatcc
      781 tgtttgctcc ccacgctttc gcacatgagc gtcagtacat tcccaagggg ctgccttcgc
      841 cttcggtatt cctccacatc tctacgcatt tcaccgctac acgtggaatt ctacccctcc
      901 ctaaagtact ctagttgtcc agtctgaaat gcaattccca ggttaagccc ggggctttca
      961 cacctcactt aaacaaccgc ctgcgtgccc tttacgccca gttattccga ttaacgctcg
     1021 caccccccgt attaccgcgg ctgctggcac ggagttagcc ggtgcttctt ctgtgattaa
     1081 cgtcaatcga ctgttctatt aaaacaatcg ccttcctcgt caccgaaaga actttacaac
     1141 ccgaaggcct tcttcattca cgcggcatgg ctgcatcagg gttcccccca ttgtgcaata
     1201 ttccccactg ctgcctcccg taggagtctg gaccgtgtct cagttccagt gtggctggtc
     1261 atcctctcag accagctaga gatcgcgggc ttggtgagcc tttacctcac caactaccta
     1321 atcccacttg ggctcatctt atggcaggtg gccaaatggt cccacccttt agtcctaaga
     1381 cattacgcgg tattagctac cgtttccagt agttatcccc ctccataagc cagattccca
     1441 agcattactc acccgtccgc cactcgtcac ccaaggagca aactcctatg tgctaccgtt
     1501 cgacttgcat gtgttaagcc tgccgccagc gttcaatctg agccatgatc aaactcttca
     1561 attcaaaaag tttaatcgct caatactgct gacataaaat cgcacttcca aatttaaacc
     1621 taattaaaat taagttaaaa gaaagtaatg aatttctagt tagcacctat taagacttca
     1681 aaattaaaaa aatgtttttc aatcaagtca atcaacaagt gcccacacag attgtctgat
     1741 aaattgttaa agaacaaaaa gaaacgacgc actggaaatc aattttaaat cgttcacaac
     1801 agtgcgtcgt tgtgtgctgc gtattatagg gaaaaacaaa aacaacgcaa gcggtttttg
     1861 taaaaaattt gaaaaaaatg accgcttgcg tatttttacg gcaaaacgag tattaattta
     1921 gctaaacttg cctcttattt gttcttttag cccactttgt ctttcggttt tggcaccaac
     1981 actgtatttc caaacttttt cgctaccaaa taaggtaaat ttatcttttg cccttgtgat
     2041 tgcggtgtaa atcaactctt tcgtcagcac cggggaaatc gaatggggca acaccaatac
     2101 cgtatgttca aattcagaac cttgcgattt atgcacagtc ataatataag ctgcttcata
     2161 atttgaaacc ctacctagag gcaaactatg gtgttctcta ttttcctgta aggaatcaaa
     2221 ataaactcgg ctttctccat tttcgtccgg caaaatgatg ccgacatcac cgctggctat
     2281 gctatttatc ggcatatttt ctgtcattaa aatcggtttt ccgatatagc ggtctcggct
     2341 atgattaaat ttcactaagc tagctttccg caagccttcc gcaatcgcca tattaagacg
     2401 ttcaaccccc agttcactta cccttaaggc tgataaaatt cggacttttt gaaatttctc
     2461 aaaaatcgct ttcacactca ctgaatctgg atttttctcc cgtttttgaa ccgcttgtaa
     2521 ataatcacga taaagttcaa ccgctttatc caccaccatt tgcacacttt tttcaatcca
     2581 gcttgattta tccgttgtct ctttcgtttc cggataacag aaattttcta acttatcact
     2641 gtttttatat ttgtcgaaaa tctcccacga aaggttaatt ttttgatcat ttactgcatt
     2701 tgcaagctct ccaatccagg ctttttcgcc aaatcgatag ctatgtttta agtgagataa
     2761 acaatcacca atcggcaacg cattaccggc ttcaatttgg tagtcggtaa cggcttttaa
     2821 gtaatcgcaa tgggattggc tataaccatt atcaataaaa gagccaagtt cccccataat
     2881 tgaaccagct tccacagaag caagctgatc tttatctccc agcattatca agcgagagtt
     2941 tggtttgagc gcttgaacaa ctttctccat cattgataaa tcaatcattg aagcctcatc
     3001 taatacgacc aaatcatagt gccacggatt tttggtatga tatttggttt tctcagttaa
     3061 tggtctcgca ccaagcaagc gatgaatagt cactgcttca gttggaatag agcgttttaa
     3121 ctcctcagat aacccctttt cttccgccaa cttgctttcc agcgactctt tcattcttgc
     3181 tgctgctttt ccggttggtg cagccagagc gatttgcaaa tatggtttct gtagtgcttg
     3241 ctgttttaat tgcagcccga gtaataattt tactaccgta gttgtttttc cggttcccgg
     3301 cccacctgaa attaagctaa actgcttttc taatgctgtt gcaacggcaa tcttttgcca
     3361 gtcgatagtt tgagctttcc cagtgaatag ttggttgaga atgctttggt ttaattcggt
     3421 atttgcaaat ttttccgaaa aatcaaccgc ttgttgtaaa taaccggcaa tgcgatgctc
     3481 cgcttgccag tagcgataaa aatagagcaa gctgttttgg aataacatcg gtttaattgt
     3541 ttcgggagaa tagctaaaag cgatatgatt tttcagcaca ctctgccatt caagcggtgg
     3601 aatttgctca attttttgca aaatttgttc gagcaaatga taagtttgtg gcttgctatt
     3661 taattcaaaa agagaagaaa gactgagatt ttccaaacgt aatgccgtat gcccaagtcc
     3721 cacttggtaa gaaaccaacg cagcggctaa cacagctaaa tcttgctcca aaggcgaata
     3781 cccatagggt ttctgcttat tatcaataaa acgggcaaat tgataattta agtcagaaat
     3841 cgccttttct tgtttaagtt ccaataataa attcagcata atcaaacctt aaatcgacca
     3901 cctaacatta agtgataata ctaatctttt ttgcattatt ttctaaggga ttttttattt
     3961 taggcgtata atttagcctt tcttgtgat
//
LOCUS       DANLXQ010000190         3974 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.193, whole genome shotgun sequence.
ACCESSION   DANLXQ010000190 DANLXQ010000000
VERSION     DANLXQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.193"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            158..1360
                     /gene="yedE"
                     /locus_tag="PXD94_002414"
                     /pseudo
     CDS             158..1360
                     /gene="yedE"
                     /locus_tag="PXD94_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
     gene            1396..1665
                     /gene="yedF"
                     /locus_tag="PXD94_002415"
     CDS             1396..1665
                     /gene="yedF"
                     /locus_tag="PXD94_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL6287223.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCSQSINNIPIDTKNHGYTLLSVEQNGTKLRYLIQR"
     gene            1765..2109
                     /locus_tag="PXD94_002416"
     CDS             1765..2109
                     /locus_tag="PXD94_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL6287224.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLSLLLVKYCKK"
     gene            complement(2172..3119)
                     /locus_tag="PXD94_002417"
     CDS             complement(2172..3119)
                     /locus_tag="PXD94_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL6287225.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQTTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADALKAQALGADILVLTGFDEGGTLPM
                     KEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTLGAEGVWIGTAFIATEECRAS
                     EKVKNWIVEANANDLLLFRTEPHYYRSLPTGLAKKCFEMSENGATRSEIAKVMNAGTG
                     MRLGMLEGDFDNGYVSVGNGISQINKIKSVKELIDELMA"
     gene            3302..>3974
                     /locus_tag="PXD94_002418"
     CDS             3302..>3974
                     /locus_tag="PXD94_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287226.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIFKKHLSDE
                     FHKLLV"
BASE COUNT         1157 a          829 c          784 g         1204 t
ORIGIN      
        1 aaaataccta ctataaagac gcatttgtaa aatgtgcatt gatatatcgc aagttttgtg
       61 tataggaaat gaactaatta ctgtgtcaat cagtcccttt cttcatcgtt ataccctctt
      121 gcaaatccga ttaatctatc cttagttaag gtgttttatg ttagcgattt ggcaaagttt
      181 taaacaaaat tatctaatca aatattggaa tcctgttgca gcagtgatcg cagctggtat
      241 tatttcagct tattatttcg gcattactgg cacttattgg gcggttacag gggaatttac
      301 ccgttggggc ggtcatctgt taaatgcttt gggtgttgat gtttcgaact ggagctattt
      361 tcaactcatc ggcttagacg gcaccatctt tacccgcatt gacggtgtga tgattctcgg
      421 tatgtttgcc ggctgtcttt ctgccgcatt atgggcgaac aacgtcaaat ggcataatca
      481 gccacataaa aaacggatta tccaagcctt gattggcggt gcgttagccg gtttcggagc
      541 ccgtttagca atgggctgta acctcgcctc tctttttacc ggtattccgc aattctccgt
      601 ccacgcttgg tttttcacct ttgcgaccgc catcggcact tactttgggg taaaagtgac
      661 tttattaccg atgtttagag tgaaattaga gctgaaaaaa ggggcagcaa aaatcaaaga
      721 aaacgatccg aaacaagcta atcgccgttt ttgggttggt atgctcattt ttatcactta
      781 tctttctgct tccctttatg taatgcaatc ctcaatcaaa cttggttttg caatgctttg
      841 cggcttagcc tttggcttat tgattgaacg agcccaaatt tgctttacct ccgctttccg
      901 tgacttatgg attaccggac gggcttatat ggcaaaagca attattttcg gcattattgc
      961 cggcacaatc ggcgtgttct cttatattca attaggtgtt tcacctaaaa ttttgtgggc
     1021 tggcccaaat gccattatcg gcggattatt attcggattt ggcattgtgc ttgccggcgg
     1081 ctgtgaaacc ggctggatgt accgttcaat ggaaggacag gttcatttta tgtgggtcgg
     1141 cgtaggcaat gtagtcggtt caaccctgct ggcttactac tgaggcgact tagctccggt
     1201 gcttaccctt gattatgaaa aaatcaacct actcaaagaa tttgggccgg ttggcggatt
     1261 attcatcaat tatggcttat taattctttg cttaatcgct gtagtttggt gggaaaaacg
     1321 cttccttgcc aaagcgaaag caaaattagc aactgcgtaa tctttttaac ttcaaccaac
     1381 aagaggaaaa tgactatggc attcaccgtt gtacaaaaac tagataaaga tccaaaagac
     1441 atcacgcctg attacacctt agatacctta ggcgaacctt gcccttaccc tgcgattacg
     1501 atgttagaaa caatgccaca gctgcaaaaa ggagagatat tggaactgtt aagcgattgt
     1561 tcccaatcga ttaataatat tccgattgat actaaaaatc acggctatac cctattaagc
     1621 gtggaacaaa acggcactaa attacgctat ttaattcagc gttaattgca aaaaattcgc
     1681 acaaaacgac cgcttgtcgt aaaatagcaa ctatttaaga gccttgtaac gcaaggctct
     1741 tttattctaa gaggtaaaag aaaaatgaat aaatacgctt tacttgcaat attttggttt
     1801 attattggca tttatgcttt actttttcgt gaaagcacta atgatatacc gccttttcca
     1861 cattttgata aagtaagcca ttttgcctta ttctttatcc agatctggtt atctgcaaac
     1921 gcctttatcc gttcaaagaa agggatacct tattttgggc taataatttt tgccttactc
     1981 tatgctttca ccagcgaatg ggctcaggca accttcacct caacccgaga aggttcttgg
     2041 cttgatgggc tagcagatat gctaggagcc tcactttcat tattgctggt aaaatattgc
     2101 aaaaaataaa gcataaaaga ccgcttgtta tatggtttag ccatactaca agcggtcatt
     2161 ttttaccaaa attatgccat caactcatca atcaattctt tcacactttt gattttgttg
     2221 atttgtgaaa tgccattgcc caccgacaca tagccattgt caaaatcccc ttctaacata
     2281 cccaaacgca ttcctgtacc agcgttcatt accttagcaa tctcactacg agttgcccca
     2341 ttttcgctca tttcaaagca ttttttcgct aatccagtcg gcagtgaacg gtagtaatgt
     2401 ggttcggtac ggaataacag cagatcattg gcatttgcct ccacaatcca gtttttcact
     2461 ttttcgcttg cacggcattc ttcggtggcg ataaatgccg tgccaatcca cacgccttca
     2521 gccccaagcg ttaaggcagc ctgcaccgct tttttatcgg caataccacc agccagcatt
     2581 atcggcaaat ccaccttgcc cacaaattcc gccagcacat taaaactgcc tacctctttc
     2641 atcggcaaag taccgccctc atcaaaaccc gtcagcacca aaatatctgc tcccaatgct
     2701 tgggctttta aagcgtccgc caaattggga ttaggcaaac ggcagatgat tttaatgcca
     2761 gcagctttga atttggcgta aatctctggg tcaagccagc cgttatccag caccacaggc
     2821 acacgctctt caatgaataa atccaccatt aacggaatca gcgaaaaatc ataagacaaa
     2881 ataaatggta cgccaaaagg cttatcggtc aatgccttaa ccttacgaat ctcgttacgc
     2941 atacggtcaa gtacttcttc gttggaagtt ggattggtgg tttgccctgc gtgtggggct
     3001 aaaaaaccaa gcccaccagc ctcggcaacg cttgctacca gatgagcgtc cgtcagccaa
     3061 gacattggcg cttggacgat agggtgcttg atatttaaga tttgggtaat gcggttcatt
     3121 ggattttcct gtttgaataa aaagaattgg acgtagttta atggttttgg atttgagagg
     3181 gaatgcgtga aaatgggaag gtggttttcg gatttggaaa attatcaatg ctaaaatact
     3241 tgaaatacac acgccaatcc tcatatcaac aaaattgaat atttaattat ggtaaaagaa
     3301 aatgtctatt agatgggaaa acatcaagcc cttacaaggt tctcaaaata gtgcttttga
     3361 agagctagta tgccaattgg cacgccaaga atttaaatct caaggaaaat ttactcgaat
     3421 ttctgcacct gatggtggga ttgaagcaat gtgtgaattg ccagatggtt ccgtatgtgg
     3481 ttggcaagcc aaatattttt taagctcatt ttcttcatca caatggcaac aaattgaaga
     3541 ttcctttaaa gaatctttaa aaaactatcc taaactaacc aaatactatg tttgtgtggc
     3601 aaccgataga gcaaatgcca atgtatctgg aaagaaatca tttcttacca aatgggagga
     3661 gtatacccaa agatggaaag actttgctca atcacaaggt cgtgaaatag aatttgaatt
     3721 ttggggaagt tttgaattat ctgacttatt atcaaaacct gaaaatgcag gcaaaaaatt
     3781 cttttggttt aacgaaagcg aattatctga caaatggttt gaacaataca atcaacaagc
     3841 cattaataat cttggtgtgc gttatacacc tgaaattaat gttgatttac ctattgcaat
     3901 gcaattagaa agcttggcaa gaacccaaat tttcaaaaaa catctgagtg atgaatttca
     3961 taaattgtta gtag
//
LOCUS       DANLXQ010000191         3866 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.194, whole genome shotgun sequence.
ACCESSION   DANLXQ010000191 DANLXQ010000000
VERSION     DANLXQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.194"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>3866
                     /locus_tag="PXD94_002419"
     CDS             <1..>3866
                     /locus_tag="PXD94_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDL6287227.1"
                     /translation="WEIYDLAPILSKMLGVEISFDKWALYDVARYCDQLVPDGMGGME
                     PRFTCNVWLTEVKTAYDLLNDFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVG
                     GFERSYSARKSRHNAVQVTYSDKTNGYESAIEYVSDDEEIKKHGLNLSQITAFGCTSR
                     GQAHRTGKWILETEKREKETITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAIN
                     GRKVTLDREISIDNASYFTYINGEATHSSKIQSVNGKEITLDSTPTGLETYGVWSLST
                     KQISSGLYRSISIVENADGTNTITALQHEPQKEAIVDNSAHFVETARTLYKAPQINAV
                     EVSTGYDGKLYITGDISSGDGKLTYDIKITKDGNLYQYKKGLSDPNIELSDLPNGDYT
                     VIIYGKNAKGQIVTEKTQTFTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAA
                     ETDNFALAKKIAKVTARTYAHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVD
                     LDARLTELNTQLSRNIVNEVFDVAAPARGLELVKTVANLTDKGTKLASSQVYNQADGK
                     LYTWNGTAYSATVAAEDVTGKLSKSKIDTSLISQLTGADNTANLARRLAETAQSNINQ
                     EITNRQDAVTAEANNRTKAIQAESANLTKKIQAEATARGAAVTQLQNVDAQQAQLISA
                     VTAKADNALSGLEEEKTARANADKAESQAREALTARMGAAESNIVTIQRTVANNAQSI
                     SEVSQNLNAKIDNINIGGRNLLRDSEFNAYNKWGSPQIEFAENANRRTIKVTSTGTNG
                     PVGIVSSNRHSTSYFQQGETYTLSLFARGSKALDYLYLMRQDGNNVRLPVINVASETE
                     FNHYKLTFKAPFTTQQGYVLIGFRQTSTEQFVEFHSVKLEKGNVATDWTPAPEDVESA
                     VSAVSADLTSYKQTQAATDSAQAQQLNQLSVNLTKAESNLNAKITEEKKARVDADKVN
                     ADKLTDITSRVANAESTITNFQSTKANKSEVASIAQQNLQSIWRTDAQSAVDALKIGG
                     ANLLVDSEYLTTARWGGSSRVASSQYGDRRLTQVFVTQAGTGHFGVTQGTQKATTRIR
                     QGETYTLSLNAQGTAGFTRTGLNYVYLIREDGGNFRLPTLPLTASLSQRPKVTFTAPW
                     TSNQVRLLIGANGIFEATDWFAFHSVKLEMGNVATGWTPTAKDIDDKVSAVQSNLTAY
                     QAAQAKADQAKATQISGLTTRMGAAESNLTRTERAVTELNQTTVTTLRDLTARTKTTE
                     GSLSRLETAKANKTEVASIAQSSLQS"
BASE COUNT         1220 a          834 c          888 g          924 t
ORIGIN      
        1 cttgggaaat ttacgattta gctccgattt taagcaaaat gcttggggtt gaaatcagtt
       61 ttgacaaatg ggcgttgtat gatgttgctc gctattgcga tcaacttgtg ccggacggta
      121 tgggtggaat ggagcctcgt ttcacctgta acgtatggct cacggaagtt aaaactgcct
      181 atgacttgtt gaatgatttc tgctcggtat tccgtgcgat tccgatttgg accggaacag
      241 aagtatcggt cattatcgac cgtccgcgtg atccggtttg gacttacaca aatgctaatg
      301 tagttggtgg atttgaacga tcctactctg ctcgcaaatc acgccataat gcagtgcaag
      361 ttacttattc ggataaaaca aatggctatg aaagtgcgat tgaatatgtc tctgatgacg
      421 aggaaatcaa aaagcacggc ttaaatttaa gtcaaattac tgctttcggt tgtacctctc
      481 gggggcaagc acaccgtacc ggaaaatgga ttttggaaac cgaaaaacgg gaaaaggaaa
      541 ccattacttt taccgtggga cgtgagggct taatgcactt gccgggcgat attattcgtg
      601 tggcagacag ccattatgcc ggtacggaaa ttggcggacg tgtactggcg attaatgggc
      661 gaaaagtcac gttagatcgt gaaattagca ttgataacgc cagttatttt acctatatca
      721 acggtgaggc aacgcactct agcaaaatcc aatcggtaaa cggcaaagaa attacgctgg
      781 atagtacacc aaccggctta gaaacctacg gtgtttggtc gttatcgact aaacaaatca
      841 gctctggatt atatcgctca atctcgattg tcgaaaatgc ggacggcaca aacactatca
      901 cagcgttaca acacgaaccg cagaaagagg caattgttga taactccgct cactttgtgg
      961 aaacggctag aacactctac aaagctccac aaattaacgc agtggaagtc tcgaccggtt
     1021 atgacggtaa gctctacatt accggtgata ttagctccgg tgatggcaag ctaacctatg
     1081 atattaaaat taccaaagat ggcaatttat atcaatacaa aaaaggctta agcgatccga
     1141 acattgaact aagtgattta cctaacggcg attataccgt cattatttac ggcaaaaacg
     1201 ccaaagggca aatcgtcacc gaaaaaacac aaacctttac tattgatcga cctccagctc
     1261 cgaccggtgt agtagtaaca ggtggattag ggcaaattac cctcgaatgg gattgggtaa
     1321 atgaggttac gcaaaccgag attttcgcgg ccgaaaccga taatttcgcc cttgcaaaaa
     1381 aaatcgcaaa agtgaccgct agaacctacg ctcacacact aaaaggtaac aaggttgtac
     1441 gctattattg gctacgtcat accagaggca ttaatgtcgg accgttttat caacaacagg
     1501 gggtaagagg acaaacagcg gttgatttgg atgctcgatt aaccgaatta aacactcagt
     1561 tatcacgcaa tattgttaat gaggtttttg atgttgctgc accggcaaga ggtttagaat
     1621 tagtgaaaac agtcgctaat ttgaccgaca aaggtactaa actggcgtca tctcaagttt
     1681 acaatcaggc tgacggcaag ctctatactt ggaacggtac agcatactca gctacagtgg
     1741 ctgcggaaga tgttaccggc aaattatcaa aatctaaaat tgatacgtcc cttatttcgc
     1801 aattaacagg tgcagataac accgcaaatt tggcaagaag attagcggaa acagctcaaa
     1861 gcaacattaa ccaagagatt actaaccgac aagatgccgt tactgccgaa gcaaataacc
     1921 gtactaaagc cattcaagcc gaaagtgcga atttaacgaa aaaaattcaa gctgaagcaa
     1981 ccgctcgtgg ggctgctgtt acacagttac aaaatgttga tgctcaacaa gcacagttga
     2041 tttctgctgt cacagcaaaa gctgataatg ctctttcagg cttggaagaa gaaaaaacag
     2101 cacgagcaaa cgccgataaa gcggagtctc aagcacgtga agcattaaca gcaagaatgg
     2161 gagcagctga aagcaatatc gtaactattc aacgtacggt agcaaataat gcccaaagca
     2221 ttagtgaagt cagtcagaat ttaaatgcta agattgataa tattaatatc ggcggtcgga
     2281 atttattaag agacagcgag ttcaatgcgt acaataaatg gggtagccca caaattgaat
     2341 ttgctgaaaa tgcaaatcgc cgaactatca aagtaacctc aacaggtact aacggtcctg
     2401 ttggtattgt ttcatctaat cgccattcca catcttattt ccaacaagga gaaacttata
     2461 cactctcatt atttgctcga ggaagtaagg cactagacta tctctatcta atgcgtcaag
     2521 atgggaataa cgttagatta ccagtgatta acgttgcctc tgaaactgag tttaatcatt
     2581 acaaactcac gttcaaggct ccttttacca cgcaacaagg ttatgtctta atcggttttc
     2641 ggcaaaccag cacagagcag tttgttgagt ttcacagcgt caaacttgaa aaaggcaacg
     2701 tagccacaga ctggacacca gcaccggagg atgtggagag tgcggttagt gctgtttcgg
     2761 ctgatttaac gagttataaa caaactcaag cggctaccga ttcagcacaa gcacagcaac
     2821 ttaatcagtt atctgtgaac ttgaccaaag cagagagcaa tttaaatgcc aaaatcacgg
     2881 aagaaaaaaa agctcgtgtt gatgcagata aagtgaatgc agataaactc acagacataa
     2941 caagccgtgt tgcaaatgcg gaatcaacta ttaccaattt ccaatcaacc aaagcgaata
     3001 aaagtgaggt ggcaagtatt gcccagcaga acttacagag catttggcgc accgatgcgc
     3061 aaagtgcggt ggatgccttg aaaatcggtg gtgcaaatct actggttgat agtgagtatt
     3121 tgacgacagc ccgttggggc ggtagctcac gagtggcaag ctcgcaatat ggtgatcgtc
     3181 gcttgactca agtgtttgtg acccaagcag gcacagggca ttttggtgtg actcaaggca
     3241 cacaaaaagc cactacgcgt attcgtcagg gtgaaaccta taccttgtca ctaaatgcgc
     3301 aggggacggc aggttttacc cgcacagggt taaactatgt gtaccttatc cgcgaagatg
     3361 gtggcaattt tagattgcca actttgccgc ttactgcatc attgtcccaa cgccctaaag
     3421 tgacatttac tgcgccgtgg acgagcaatc aagttcggtt actgattggc gcaaatggca
     3481 tatttgaggc gacagactgg ttcgcatttc acagcgttaa gctcgaaatg ggtaatgttg
     3541 ccacaggctg gacaccaacc gccaaggata ttgacgacaa agtatcagcg gttcagtcca
     3601 acctcaccgc ttatcaggcg gcacaagcta aagcagatca agccaaagct acgcagattt
     3661 cggggctcac aacccgaatg ggggcagcgg aaagtaacct cactcgcacc gaacgggcag
     3721 tgacggaact taatcagaca acggtgacga cactgcgtga tctaaccgcg cgcaccaaaa
     3781 caacggaagg ttcgctctct cgtttggaaa ccgccaaagc aaataaaact gaggtggcaa
     3841 gtattgctca aagctcctta cagtca
//
LOCUS       DANLXQ010000192         3816 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.195, whole genome shotgun sequence.
ACCESSION   DANLXQ010000192 DANLXQ010000000
VERSION     DANLXQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.195"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            113..694
                     /locus_tag="PXD94_002420"
                     /pseudo
     CDS             113..694
                     /locus_tag="PXD94_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            695..865
                     /locus_tag="PXD94_002421"
     CDS             695..865
                     /locus_tag="PXD94_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL6287228.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLMKEFNS"
     gene            complement(906..1979)
                     /locus_tag="PXD94_002422"
     CDS             complement(906..1979)
                     /locus_tag="PXD94_002422"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL6287229.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
     gene            2247..2930
                     /locus_tag="PXD94_002423"
     CDS             2247..2930
                     /locus_tag="PXD94_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL6287230.1"
                     /translation="MNQTRDRLEKLPLFHKMQQFIIKALKIIAIFPLLGIAILLVVDS
                     LTSYIVRDKIYTDSKQIPYREYAVVLGTAKFYSKDVINQYYKYRLEAAKQLVQEGKVN
                     QLLLSGDNKTPYYNEPKTMTSDLLKMGIATSKIKQDYAGYTTFYSIIRAKKVYKLPPF
                     TIISQKFHCERALFIAKFRDIDAICYAATYPEGAYQVRMREILARSAMVFSLLIGKEP
                     ESLEDIRSK"
     gene            complement(2967..3806)
                     /gene="purU"
                     /locus_tag="PXD94_002424"
     CDS             complement(2967..3806)
                     /gene="purU"
                     /locus_tag="PXD94_002424"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL6287231.1"
                     /translation="MLQQHKILLTECPDDTGLVAKITNICYKHQLNIIKNSEFVENET
                     KRFFMRTELNGIFNDETLLTDLKFTLPEGSSYKLLPKQRKRIVILVTKEAHCLGDLLM
                     KNYYGGLDVEIAAVIGNHETLKSLVERFDIPFHLVSHENLTRVEHDKLLAEKIDEYSP
                     DYIVLAKYMRVLNPEFVARYPNRVVNIHHSFLPAFIGAKPYHRAYERGVKIIGATAHF
                     VNDELDEGPIIMQNVINVDHTYTAEAMMRAGRDVEKTVLSQALELVLADKVFVYKNKT
                     IIL"
BASE COUNT         1200 a          671 c          757 g         1188 t
ORIGIN      
        1 gtatgttagg tttaaaaata cttcatacca atatccccta agttgggtaa aaccttatga
       61 aataggatac aagcggttag tttctctcaa ttttttacca aaggatcacg caatgagcca
      121 accggcttta cggcaaattt ccccctcgca gctagaagat attcgtctgc aaatggtgaa
      181 gtttgccttt ttacagttaa aaaataacga acttgccgaa gatgtggtgc aagaggcgtt
      241 tgtcagcgct tataaatatg ccgacagctt caaaggtgaa agtgcgttaa aaacgtagat
      301 ttttgcaatt ttaaaaaaca agatcgtaga tttagtgaaa gccaaaggca aattggtggc
      361 agtatctgag ctgatggaag aagacgatca cgatcttgcc gataaattgt ttaacgaaaa
      421 tggcggttgg gataaagatg cttatcaatc taatgagtgg aagaacaccg aaagccatac
      481 ctattccgct caattctggc aagtatttga attttgccta aataatctgc cggcaaatca
      541 agcaagggtg tttatgatgc gttcccattt ggaaatggaa accgaagaaa tttgcgcaga
      601 atgtgagatt tcaccagcaa atgtgcatac cttactgtat cgggcaagat tgcagttaca
      661 ggtctgttta agtcaaaaat ggtttggagg ttaaatgtta aaatgtaagc aaattaccga
      721 attagtttct ctgtcccaag aacaaaaact gacattaaaa caacgtgtta atgtgcaagt
      781 tcatttaatg ctttgttctc gttgcagagc ctttagccaa aactgcaaaa ccgtagataa
      841 gctaatgaaa gaatttaact cttagcgtaa aagcaagcgg taaaattttt aataaaaaat
      901 gcaatttatt ttgctaatcc ggctttaatc atcggttcaa aaaagtcata atcaaccaaa
      961 aaggcatcgt gtccgaaatc ggagtgaaac tcgtggaact ccaaatttac gcctgcctgt
     1021 tctaattttt gtttgctctt atacacgtca acttgcttga atagttggtc gcttgttacc
     1081 gctacaaaag tgtagttggc tttaatacgt tttagggcgg tttcttcatt ttcatagcca
     1141 agagccgggt cgtataaatc taatgctcgg agtaaacgga ggtaagtgtt ggcatcgaaa
     1201 cgacctaaaa atttcgtgcc ttgatagctt aaataggatt ccacttggaa atggtcgccc
     1261 caaatttgtc cttgctgttt ggtttctcgt ccaaaggctt tagcaagttg aatatcagta
     1321 cggtaagtca gcataccgag catacgggcg atttttaagc cgttatcagg tgcaatgcca
     1381 tcataatagt tcccaccgtt aaagtgtgga tcgttaataa tagcttggcg cataacgtgg
     1441 ttaaaaccga tagcctcggc acttagggta agtgacgaac aaagatttat aatgttgcga
     1501 actttatcag ggtaactaat accccactgt gttgcttgca ttccgccgaa agatccccct
     1561 accactgcgt gaagttgagg gatttctaaa tagtccaata atgctttttg tacattaaca
     1621 atatcttgca ctgtaataat tggaaacaaa ctaccgtaag gtttattggt tttagggtta
     1681 atcgaagctg ggccggttgt gcctttacaa ccgcctaata cattactgca aataaagaaa
     1741 tatttatcgg tatcaaacgc aagccctttg ccgatgaaat cttgccacca gcctttctcg
     1801 gttggtgagt catagtaagg cttagcatca ccggtaaggg cgtggcaaag caagaccgca
     1861 ttgcttttct ctgcatttaa tctgccgtaa gtctggtaag ccactgtgat aggggacagt
     1921 tcgcctccta atgtgagtgg taaaggtacg tcctcaaaaa gggtaatctg ctgagccata
     1981 aaaatcctgt ttgcaaaaaa ttctgctttt ttgaccgctt gttgtaatga ccgttaaatt
     2041 agcggtgttc atttcaattg tcaagaattt ttagacgtct aaacgtctag atttttaaac
     2101 ggctaaaatc aaaattttat tgctgagaag atatttatta cttaacaaat aacatgaaat
     2161 agaataaatt tattgaacga ttagactatt ttgtttgtct ctaatttacc catcttaaat
     2221 agggtagcaa aagtaaagga tctcatttga accaaacgcg tgataggtta gaaaaattgc
     2281 cacttttcca taaaatgcag cagtttatta ttaaggcatt gaaaataatt gcaattttcc
     2341 ccttattggg aatagctata ttattagtgg ttgattcact tacaagttat attgtgaggg
     2401 ataaaattta tacagatagt aaacagattc cttaccggga gtatgcagtt gtactcggta
     2461 cggcaaaatt ttactctaaa gatgtgatta accaatatta taaatatcgt ttggaagccg
     2521 ctaagcaatt agtccaagaa ggaaaggtca atcagctttt gttaagtgga gataataaaa
     2581 cgccgtacta taatgagcca aaaacaatga caagtgatct acttaaaatg gggattgcga
     2641 cttcaaaaat taagcaggat tatgcggggt ataccacttt ttattcaata attagggcta
     2701 aaaaagtata taaattaccg ccatttacga ttatctccca aaaattccac tgtgaaaggg
     2761 cattgtttat tgcaaaattt agggatattg atgcaatttg ttatgccgct acatatccag
     2821 agggggctta tcaggtacga atgcgggaaa ttttagccag atcggcaatg gtttttagtt
     2881 tgttgatagg taaagagcct gaatcattgg aagatatccg ttctaaatga gatagccggt
     2941 cagataatgt tgaccggcta ttttgcttat aaaataatgg ttttgttttt atatacaaat
     3001 actttgtcag caagcactaa ttctaaggct tggctaagta cggttttctc gacatcacgt
     3061 ccggcacgca tcatagcctc tgcggtgtag gtgtgatcaa cattaatcac attttgcata
     3121 ataatcgggc cttcatctaa ttcatcattc acaaaatggg cggttgcacc gataattttc
     3181 actccacgtt cgtatgcacg atgataaggt tttgcaccaa tgaaagcggg taagaacgag
     3241 tggtgaatat tgacaactcg gtttggataa cgagcgacaa attccggatt cagcacccgc
     3301 atatatttag ccaacacaat ataatcaggt gaatattcat caattttttc cgctaataat
     3361 ttatcgtgtt ccactcgggt taaattttcg tggctgacta aatggaatgg aatatcgaaa
     3421 cgttcaacta aactttttaa tgtttcgtgg tttccaatca cggcagcaat ttctacatct
     3481 aatccaccat agtaattttt catcagtaag tcacctaaac aatgtgcttc tttggtaact
     3541 aaaataacga tacgttttct ttgtttaggg agtaatttat agcttgagcc ttcaggtaag
     3601 gtaaatttta aatcagtgag taaggtttca tcattaaaaa ttccgtttaa ttcggtacgc
     3661 ataaaaaaac gtttagtttc attctcaaca aattcagaat ttttaataat atttaattgg
     3721 tgcttatagc aaatattggt aattttagcg acaagtcctg tatcatcagg acattcagtt
     3781 aataatattt tgtgttgttg tagcatttca tttcct
//
LOCUS       DANLXQ010000193         3808 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.196, whole genome shotgun sequence.
ACCESSION   DANLXQ010000193 DANLXQ010000000
VERSION     DANLXQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.196"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            254..586
                     /locus_tag="PXD94_002425"
     CDS             254..586
                     /locus_tag="PXD94_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL6287232.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
     gene            651..1742
                     /gene="trmA"
                     /locus_tag="PXD94_002426"
     CDS             651..1742
                     /gene="trmA"
                     /locus_tag="PXD94_002426"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL6287233.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPETKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLDQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            1834..1923
                     /locus_tag="PXD94_002427"
     tRNA            1834..1923
                     /locus_tag="PXD94_002427"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1868..1870,aa:Ser,seq:tga)
     gene            2079..2450
                     /locus_tag="PXD94_002428"
     CDS             2079..2450
                     /locus_tag="PXD94_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL6287234.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXD94_002429"
     CDS             complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXD94_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL6287235.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(3425..3715)
                     /locus_tag="PXD94_002430"
     CDS             complement(3425..3715)
                     /locus_tag="PXD94_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL6287236.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
BASE COUNT         1142 a          833 c          733 g         1100 t
ORIGIN      
        1 cacagtatat ctccaaatat taaagttaat caaaactcaa tacatttagg taatatacct
       61 taacaaatat aaaaaatata cctcagactg cattgtctat tttaaaagga tcaatatttc
      121 tccttaaaga gttttttgca aaaaatttgc caaatttgac cgctttttta cattaaggca
      181 ttgatttatt cgctgttttt gtttaatgta tgccgatttt attttcccta atttcaaaca
      241 acgaggcatt attatggcag acagtttcag cgttacgcgc cgcttttttg acgataaaaa
      301 ttatccacgt ggtttctctc gccacggcga ttacaccatt cgtgaatcac aaactttaga
      361 acaattcggt caagcttgct tggctttaga aagtggtgaa cgtaagccgg caactcaaga
      421 agaaaaacgt ttcgttgcgg taatgaaagg cgaagaggta gcagaaactg acattgaaaa
      481 agcgtggtta aaataccgca cattaactag taaaacaaaa cgcatctaca ccctttcagg
      541 caatgcaggt agcgatgccg gcgatgactt cagtgctgct gaataatttc taatttaaaa
      601 ccacgaagta attcgtggtt tgttttttca tttgctattt gtggtaaaaa atgactcttc
      661 ctatcgaaca atattccgat cttcttgcaa aaaaaaccga aaatttgacc gctttactca
      721 agccgtttaa cccgcctgcg attgaggtat ttgcctcaaa gccaagccat ttccgtatgc
      781 gggcagaatt tcgagtgtgg cacgaggaag gcgagctgta ccacattatg ttcaaccctg
      841 aaaccaaagc tcgttatcgt gtggatagct tccctattgc cagtgagctg attaaccata
      901 tgatgaccgc attattggcg gaaattaaag gcaatgaact actgactcgc aagctgttcc
      961 agatcgatta tttaagcacc ttaagcggtg aaattgcagt ctcaatgctt taccacaaaa
     1021 gcctgaatga agaatgggtt gaacaggcaa acgccttaaa aagccgttta gagcaacaag
     1081 gtttcaaagt gcaaattatc gggcgtgcca ataaacaaaa aatcgcactg gattgcgatt
     1141 atgtggaaga aattctaccg gtacacggca aaaattatgt gtatcgccaa gtggaaaaca
     1201 gctttaccca gccgaatgcg gaaatgaata ttaaaatgct ggaatgggcg agaaattgca
     1261 caaaaaatag tgaaggcgat ttgcttgagc tttactgcgg caacggcaat ttttcgattg
     1321 ccttggcgga aaacttccgc aaagtgctgg caaccgagat ctcaaaatct tcagtgcaat
     1381 cggctcaata caatattgaa aaaaatggca ttgttaacct acaaattatc cgaatgtcgg
     1441 cagaagaatt tacccaagcg atgaacggtg tgcgagcctt ttatcgctta aaagggattg
     1501 atttaaacgc ctacgaatgc aataccattt ttgttgatcc acctcgtgca gggttagatc
     1561 aagctacatt aaatatggtg caagcgtatg agcggattct ctatatctct tgcaatccgg
     1621 aaacccttgc cgagaattta caacaattaa gccaaaccca tcggattgaa aaagccgctc
     1681 tttttgacca attcccttac acccaccata ttgaaagtgg cgtttggtta attagaaaat
     1741 aaattgtgct gaaaaaaaga acgaataagc agcttttaag atttattact tgacgatgtt
     1801 cgcaataatc tctattattg caacgttttc aacggaggaa tggcagagcg gttgaatgca
     1861 ccggtcttga aaaccggcga gggttaacgc cctccgtgag ttcgaatctc acttcctccg
     1921 ccagtcttta aacctgttga gcaatcagca ggttttttta tttcatttgc aagaaaatca
     1981 gataaaacca ccgcttgaat ccatcgctat aaactggttg aaatcagcct aaagttgctt
     2041 taggataatt gcgattcatt atcaataact taattattat gaaatacctt tatattatct
     2101 gtggttttat ttgtattggt ttaggcatca tcggggcagt attaccgggc ttgccgacca
     2161 caccgttctt acttttggct ctgttctgct ttactaaagg ctcggagaga atccgccagt
     2221 ggtttattca aaccaaatta tacaaaaaat atctgcaaga atatgatgag aaacgggcaa
     2281 tgacgatgaa gcaaaaaatc acgattttag cgatttcgtt tccgtttaca ctatttgctt
     2341 tctttgcact gccccatatc ggtgggaaaa ttgcgttagt agtgttagtg ataattcaat
     2401 attggtattt cttctgcaaa atgaaaaccc ttgaaccgga gaatgcctaa tcttcctgct
     2461 tccgccttgc tcttgggtgg gcagagtcat aaattttagc taagtgttgg aaatccagat
     2521 gcgtataaat ttgagtggtg gacaggctac tatgacccaa taattcctga accgctcgca
     2581 aatcaccgct tgcctctaac atatgagtcg caaaagagtg acgtaattta tgtggatgca
     2641 gatggctttc cagcccttgt cgctcacccc atttcttcat tgccaactga atggaacgat
     2701 gagataaccg cccacctcgc ttattcagaa acaccgcatt atcttgcggc ttaaattgca
     2761 aacgcatttc taaccagcga tttaacgctt ttaacgcttg cgagccaatc ggcacaattc
     2821 gctctttgct acccttacct tgcacccgta cttctcgtgc cgataaatcc atatcgccta
     2881 aatccagccc ttgtaattcc gataaacgca agccggaact atacatcagc tccatcatcg
     2941 caatatctcg aatatcgctc ggctcatcac tctcaatcgc cagcaactgc ccgacctgct
     3001 ccgcatcaat atttttcggc aaatgcttac ctactttcgg cgatttcacc cctaaagtcg
     3061 ggtttgccga cattcgcccg attttaatca aatagacaaa ccattgccgt aacgccacca
     3121 agcgtaagcc gatacttttc gcactcaacc cttgcttatg gctttggctt aacatccaac
     3181 gcaccgagct actttcgacc tcttcccaat tttcaatgcc tgcttgcgaa aaaagctcgc
     3241 tcaccgccat taattggcgt tgataattgc ttaaggtatg cgggctaact tgcttttcaa
     3301 tgcgtaagaa atcccaatag gggcgggttt gttggaaaag agggttggaa gtggtcataa
     3361 acgtaataac aagcggttaa tttttaggaa aatgttgcaa gggcaaaaga tttgcccttt
     3421 gagattaggc tagccccaac tgaccgatac gcacgccaac attaccaagt gcaacagggc
     3481 ttaaatattc gccgagaaat tcaaacaatt cattcacatc agtaaaactg cgatgagttt
     3541 tcacttcttg ctccacttta cgagtaaatt cataatgtac attttcgccg ttaataaagg
     3601 cttccagcgt taaatatacg gtttcaaaat agtggcttgg cgagccttca aagcccggat
     3661 cactcactcg aacattccaa tgattactaa ataaaacttc ggtttttact gacattgtgt
     3721 tttccttctt cctataagta cgtacattat tttaatcaag atagattgaa tttgccactt
     3781 ttcctagtca gaatcgaggc aaatctta
//
LOCUS       DANLXQ010000194         3799 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.197, whole genome shotgun sequence.
ACCESSION   DANLXQ010000194 DANLXQ010000000
VERSION     DANLXQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.197"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(132..1634)
                     /gene="ppx"
                     /locus_tag="PXD94_002431"
     CDS             complement(132..1634)
                     /gene="ppx"
                     /locus_tag="PXD94_002431"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL6287237.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
     gene            1830..2159
                     /locus_tag="PXD94_002432"
     CDS             1830..2159
                     /locus_tag="PXD94_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL6287238.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            complement(2223..3653)
                     /locus_tag="PXD94_002433"
     CDS             complement(2223..3653)
                     /locus_tag="PXD94_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL6287239.1"
                     /translation="MSTLTYLQKIGRALMVPVAALPAAALLMGIGYWIDPDGWGTNSQ
                     LAALLIKSGAAIIDNMSILFAVGVAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQ
                     LKGIAVDQVPAAFGKINNQFIGILVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVV
                     SFILLYVWPVIFGALVSFGESIKDMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVA
                     GINDIPNFLGGAKSLAEGKAVIGVTGMYQAGFFPMMMFGLPAAALAIYHSAKPAKKMQ
                     VASIMFAAALASFFTGITEPLEFAFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGF
                     SAGLVDLVLSTRNPLAVQWYMLLAQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQ
                     TVNTVSRSEQAAKFVEALGGKENITSIDACITRLRLTLNDTAKADETKLKALGSKGNI
                     KLGENGYQVILGPEAEFVADAMKKLN"
BASE COUNT         1236 a          857 c          656 g         1050 t
ORIGIN      
        1 acatcaaaaa tgagaagttg atcaaatttt aaaaaatact ttttgcaaaa aattagctaa
       61 atttgaccgc ttgttcgcaa aagggaatgc aataaacgaa aaaggaagcc ttcgcttcct
      121 tcatttaaat gttaagagac cgcaaactga agatttgtac tcgccaatat tttttgctct
      181 tcttctaaat ctattgaaat cagtggatta ttcgctaaga agccctgttc aaaggtaagt
      241 tgccaagcat tattttcaat caccaaattc aactgtttcg gcactacagt cgcttggcga
      301 gaacggttta atagcaccgc tagccggaaa acccgtacca acgttaatac atctctacat
      361 tggtaacggt tgatactacg aatatccgag cgtttgaatc ctttaaggtg gtatcgcatt
      421 aataccgata gaagggtttg ctgttcaaaa tcaaatcccg gtaaatctcg gtttgcaatc
      481 aaataagcag aatgacgatg cacattattg tggtttacaa caataccaat ctcgtgtaat
      541 aaacttgccc atttcagcat tgaacgcaga tcttgcactt ggcggcggtt cttccaagtt
      601 ttgatttggt caaataaaat caacgccgta tgctccaccc tttttgcttg ctgtttatct
      661 aaagcaaatt gctcagctaa cgcttctgca gtccgttggc gaatatcccc aacttggaaa
      721 cttttctcca aaccatacat taccccttct cttaaagccc cgttggaata acgtaataca
      781 tcaattctaa aagtgtgaaa aagtgctaaa agaatagcta agcccggaac tagcacatct
      841 gctcgttcat caagtaagcc tcgtagctta atctcattta acgatttaaa ctctaggcaa
      901 atatcaataa ttttctgtag gcggctttct gtaattaagc catcacgata gccattggca
      961 atcagcactt gatggacggt tttaatcgta ccggaagaac ctaaagcata gtcccaacct
     1021 aactctttgt actcccacat taaatcttcg attttttcca ttgcaagctg ataggcacta
     1081 tcaaaacgct caatcgttaa ttccccctgc gggaaaaagc gttttgcaaa actcacacaa
     1141 cccatatgac gactttccgc tcgaatcggg gtaaagtcat caccaatagt catttccgtt
     1201 gaaccaccgc caatatctac cactaatttt ctgccagttt cgggttgggt atggctaacg
     1261 cccgaataaa tcagcctcgc ctcttgttgt ccggaaataa tggaaatcgg gtaaggaaat
     1321 actgctttcg cttgctccag aaaagcttgg ttattgattg ctctacgtaa ggtataagtg
     1381 ccaaccacct tcacattctc tgcagagaac ccttctaaac gttttgcaaa taaagctaaa
     1441 caagcaatgc ctctatcaat cgcatcttgg cttaatacta agttttcatc aagcccatct
     1501 gccagccgta cacgccgttt caaacgggaa agcacttgaa tggaaccatt tacgatacgg
     1561 gctacaatca tatgaaagct gttcgagcct aaatcaatag ctgcaaactc tctaggagca
     1621 atatttgatg acataatgaa accctgtacc aataactgaa ataagtaaga tattaccatt
     1681 tttgagccct agatatcact acaaatttag cgttattcgc gagaattaat ttaaatcata
     1741 aaatgtgata aatttttttg acgaattgac ggaaaaagaa taggatttac gagcttactt
     1801 aattcaacac tacatcaagg gagattgata tgtctgaagt gaataaatca ttccacgata
     1861 tgctggaata tgtgcatctt tatcgtctaa aaaacaaact gcaccgtgaa acggcggata
     1921 atgatcgcaa aattcgagat aatcagaagc gtgtactttt attagataac ctaacgcaat
     1981 atattaccga cgaaatgtct atcgaggaag ttcgagcgat tatttccaat atgcgtgatg
     2041 actatgaaaa tcgagtagat gattatatga tccgtaatgc ggaactttcc aaacaacgcc
     2101 gtgaaatccg ccaaaaaatg gcctctcaca aacaagcacc aaccaataaa aaagcataaa
     2161 aagccaagcg gtctgatttg caaaaaaata tgcaaatcag accgcttgtg attaagcaga
     2221 gattaattca gctttttcat tgcatctgcc acaaattctg cttccggtcc caaaatcact
     2281 tggtagccat tctcgcctaa tttgatatta ccttttgacc ctaacgcttt caatttggtt
     2341 tcgtctgctt tcgccgtatc atttaaggtt aggcgtaagc gggtaataca agcatcaatg
     2401 ctggtaatat tctctttacc acctaatgct tcaacaaatt tggcagcttg ttcgctacga
     2461 gaaacagtat tcactgtttg tacttcgcta tcttcctcac gccccggcgt ttttaaatta
     2521 aaggcttgga tcactactcg gaacaataca tagtaaatta ctgcgaatac gataccttgg
     2581 gccaataaca tataccactg cactgccagc ggatttcgtg tcgataacac taaatcgacc
     2641 aaccctgcac taaaaccgaa accggcaatc cattccattg aggctgcaat ataaacagat
     2701 aatccggtta ataatgcgtg aaccacataa agcacaggtg caacaaacat aaaggcaaat
     2761 tcaagcggtt ccgtaatgcc ggtgaagaac gaagctaaag cagcagcaaa cataatagag
     2821 gctacttgca ttttcttagc cggtttcgca ctgtggtaaa tagctaatgc tgccgcaggt
     2881 aagccaaaca tcatcatcgg gaagaaacca gcttgataca tacctgttac tcctatcact
     2941 gctttgccct cagccaatga ttttgcccca cctaagaagt ttggaatatc attaatgcct
     3001 gcaacatcaa accagaaaac cgagtttaat gcgtggtgta atccaaccgg aattaataaa
     3061 cgattaaaga aagcataaat acctgcccct aacgccccca tatctttgat agattcccca
     3121 aaagagacta aagcaccaaa aataaccggc cagacataaa gcaagataaa tgacaccaca
     3181 atcggcacca atcgtttacc gctaaagaaa gacaatgctt tcggtaactc tacttggtag
     3241 aaacggttat aaatttcggc tgaaataacc cctactaaga tcccgataaa ttggttgttg
     3301 attttaccga atgctgcagg cacttgatca actgcgatcc cttttagctg agcaacggtc
     3361 cccggagaca acaaagtggt aattactaaa tacccgacta aaccggataa tgctgctgcc
     3421 ccatcttttt cttttgacat accaaatgcc acacccacgg caaatagaat agacatatta
     3481 tcaataattg ccgccccaga tttaatcagc aatgccgcta attggctatt tgttccccag
     3541 ccgtccggat caatccaata gcctataccc atcaataatg ctgctgctgg taatgcagca
     3601 accggcacca ttaatgcacg cccaattttt tgtaaatagg ttaatgtact cataaaagcc
     3661 tccaaaaata cctcataatg agtaacttaa gcatagaaaa aaaccgatta aagaacaatc
     3721 aaataaattt ggttttgtga tccatttcaa aatatttttt aggagttttt attcaacatt
     3781 agaaatatgt ttacttttt
//
LOCUS       DANLXQ010000195         3786 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.198, whole genome shotgun sequence.
ACCESSION   DANLXQ010000195 DANLXQ010000000
VERSION     DANLXQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.198"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            20..2470
                     /locus_tag="PXD94_002434"
     CDS             20..2470
                     /locus_tag="PXD94_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HDL6287240.1"
                     /translation="MTVEVGGVVITADLEFENLLRDTQKVDKAMDDIGKSTLKAEKGL
                     KGLETQATKTAIAVNQATNKMKNIRGVAGQLGYQIQDISIMLQMGSNASTVIAQQGSQ
                     ILSIFGPMGAVAGAILAISGAIGGALLPSLFDSTDATELLAAAQKNLSEVVTQTESGV
                     SALSAKIIKLAKVSEDAAKAKIAVAMSDAKIAIKAAGDSSQKAFEQFDGFFGSFTNSS
                     QSIRNAIDELKRYESKGKDVGAVIKELGGTYGGAIAEVNALDSVTSEMSKTLGITQSE
                     ALGLIKLLAQLEQDKSPDNIKLLAIALSDLSDKNGWANEELNKLTRTINDNSVSALDA
                     ETSVRLLESAIKDLQNAIQNSQGALDGNVAKMNQLADAAERKAATIGKTERETAKYAA
                     TLLATTDEAKKNLDSTLSRIDASYDQIEAYQAEQKALQDKKTADAKAAREAEAAAKKS
                     AQAKKQVVEQLRQMATQYEIAVLRQKGMHLEAVKLEAGMRLGAAATKEQREEAEKLAQ
                     SIYGATAASQNFNAIQAQASPVTALDQRHQQQLAQIEEYKILYPQKIMEAEAARAAIE
                     EQYRQQRMEAQWEEWKQASDGARMFGDAVDAVGSSATSTITGLLNGTMSVRDAFASIA
                     NTILNSVVQSLVEMGMAQVKQMIMGQTAAKASMAAQMVQAKALAASFAPAASMVSLAT
                     QGANAIPAQAAITSTMSMAQVAAVTGRKLGGPVSARQMYRVGENNQPEIFKASNGMQY
                     MIPGQSGRVFSNKQSQGQGGGESKTVAVIVNQTNHFSSDEASANQAAVAKALSNQIKA
                     SVRLELQSQMRAGGVLAR"
     gene            2474..2803
                     /locus_tag="PXD94_002435"
     CDS             2474..2803
                     /locus_tag="PXD94_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287241.1"
                     /translation="MQTFNFKVEQDYTVQNEPSVVILKFADGYEQTAPKGLNHNLKKY
                     QGITVKANKAEAVKIQQFLNAHGGYKKFNWRDRTSDETVTVRCRSWSSTKLGAVIEFT
                     LNFDEVI"
     gene            2803..3273
                     /locus_tag="PXD94_002436"
     CDS             2803..3273
                     /locus_tag="PXD94_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6287242.1"
                     /translation="MPKAPPKKMASELIKLEQDALIELWEIDLTHIYSSSNPNQRGEI
                     FRFHNGVSQTQQNIWWQGNEYQAYPIAADGFEISGQGPSNRPTLTVSNLYGVVTVLVE
                     DFGQPVEAKVSSEETVDGNIQMTVELLKKMDQIADQRYRRNQLNDLRTGGTLSK"
     gene            3281..>3786
                     /locus_tag="PXD94_002437"
     CDS             3281..>3786
                     /locus_tag="PXD94_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL6287243.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRKLQDKNGNAGSVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLSSLTRETATFTLA
                     LPIETDNA"
BASE COUNT         1258 a          762 c          886 g          880 t
ORIGIN      
        1 taattttaag gaattgttta tgacggtcga agttggcgga gttgtaatta ccgcagatct
       61 agaatttgaa aatctgctaa gagatactca aaaagttgat aaagcgatgg atgacatcgg
      121 aaaatcaaca ttaaaagcag aaaaagggct gaaagggcta gaaacgcagg caaccaaaac
      181 ggcgatagca gtaaatcaag ccacaaacaa aatgaaaaat atccgtggag tagcaggtca
      241 acttggttat caaattcaag acatctcaat aatgcttcaa atgggttcta acgcttcaac
      301 cgttattgct cagcaagggt cgcaaatctt aagtattttc ggtccaatgg gagctgttgc
      361 aggggcgatc ttagctatta gtggtgctat tggtggggcg ttactcccta gtttgtttga
      421 ttcaactgat gcaactgagc ttttggcagc agctcaaaaa aatctcagcg aagtagttac
      481 tcaaacggaa agtggtgttt ccgccctttc tgcgaaaatc attaaattag caaaagtaag
      541 cgaagatgca gcaaaagcaa aaattgcagt agctatgagt gatgcaaaaa tagctattaa
      601 ggcagcaggg gattcatcac aaaaggcatt tgagcaattt gacggctttt ttggatcgtt
      661 tacaaattca agtcaaagta ttcggaacgc aattgacgaa ttaaaacgct atgaatcaaa
      721 aggtaaagat gttggtgccg taataaaaga acttggaggc acatatggtg gtgcaattgc
      781 cgaagttaat gcgctggatt ctgtgacaag cgaaatgtct aaaacgcttg gtattactca
      841 atctgaggct cttggtttga taaaactgtt ggctcaatta gagcaagaca aaagccctga
      901 taatattaaa ttactcgcga tagctttatc tgacttaagc gataaaaatg ggtgggcgaa
      961 cgaagagcta aataagctaa ccagaacgat aaacgataat tcagtatctg ctttagatgc
     1021 cgaaacatca gtaagactct tagagtctgc tataaaagac ttgcaaaatg caatccaaaa
     1081 ctcccaaggt gcattggatg gcaatgtagc aaaaatgaat caacttgcag atgcggcaga
     1141 aagaaaagct gcaacgatag gcaagactga gcgtgaaact gctaaatatg cagctacgct
     1201 attagctaca accgatgaag ctaaaaaaaa tcttgattcc acattaagta ggattgacgc
     1261 atcatacgat caaatcgaag cctatcaagc cgaacaaaaa gccttacaag ataaaaagac
     1321 cgcagatgca aaagctgcca gagaagctga agcggctgca aaaaaatccg ctcaagccaa
     1381 gaagcaagtt gtcgaacagt tgcgacaaat ggctacacag tacgagattg ctgttttgag
     1441 gcaaaaggga atgcatctcg aagccgtaaa attagaagct ggtatgcgtt taggagctgc
     1501 ggcaacaaaa gagcaacgag aagaagctga aaaactagca caaagtattt atggagcaac
     1561 cgcagcaagt cagaacttta acgctataca agcccaagca tctcctgtta ctgcattaga
     1621 ccaacgacat caacaacagc tagctcagat cgaagaatat aaaatcttgt atccacaaaa
     1681 aataatggaa gcagaagctg cgagagctgc aatcgaagaa caataccgcc agcaacgaat
     1741 ggaagctcaa tgggaggagt ggaagcaagc gagcgacggg gcgagaatgt ttggtgatgc
     1801 ggttgatgct gttggttcaa gtgcaacaag cacaattacg gggctactaa atggcacaat
     1861 gtccgtgcgt gatgctttcg catcaattgc aaatacgatc ttaaatagtg tagtacaaag
     1921 ccttgtcgaa atgggtatgg ctcaagttaa gcaaatgatt atggggcaaa ctgctgccaa
     1981 ggcttctatg gcggcgcaaa tggtgcaagc taaagcgtta gctgcttcat ttgcacccgc
     2041 ggcctctatg gtttctcttg ccacacaggg ggcgaatgct attcccgctc aagctgcgat
     2101 cacgtcaact atgagtatgg ctcaagtcgc cgcggtgaca ggtcgtaaac ttggcggccc
     2161 tgtatcagct agacagatgt atcgtgtcgg cgagaacaat cagcccgaaa tcttcaaagc
     2221 gagcaatggg atgcaatata tgatccccgg tcaaagcggt cgtgtattca gcaacaagca
     2281 gtcgcaaggt caaggtggag gcgaaagcaa aacagtagct gtcatcgtaa atcaaaccaa
     2341 tcactttagc agcgatgaag ccagtgcaaa tcaagcggct gttgcaaaag cattgagcaa
     2401 tcaaatcaag gcttccgtga gattggaatt gcagtcacaa atgcgagctg gtggagttct
     2461 agcgaggtaa tcaatgcaaa catttaattt caaggttgag caagactaca ctgttcaaaa
     2521 tgagcccagt gtcgtcatac taaaatttgc cgatggttac gagcaaaccg ctcccaaagg
     2581 gttaaaccac aatctgaaaa aataccaagg cattaccgta aaagcgaaca aagcagaagc
     2641 ggtgaagatt cagcaatttc tcaatgctca cggtgggtat aagaaattca actggcgcga
     2701 tagaacaagc gatgagacgg taacggttcg ttgcagaagc tggagctcca ctaaattagg
     2761 tgcagtcatt gagtttacac ttaatttcga tgaggtgatt taatgccaaa agcccccccc
     2821 aaaaaaatgg catcagagtt gataaaactc gaacaagatg ccctgattga gctgtgggag
     2881 atagatctca ctcacatcta ctctagctca aacccaaatc agcgcggaga gatattccgc
     2941 tttcacaatg gcgtgagcca aacacaacag aacatctggt ggcaaggcaa tgaataccaa
     3001 gcatacccga ttgccgccga tggttttgag atttcgggtc aaggaccgtc aaatcgtcca
     3061 acactcactg tctctaacct gtacggtgtt gtcacggtat tagtagaaga tttcgggcaa
     3121 cccgtagagg cgaaagtcag cagtgaagag actgtagatg gcaatatcca aatgacggtg
     3181 gaactgctga aaaaaatgga tcaaatcgcc gatcaacgct accgtagaaa tcagttaaat
     3241 gatttaagaa caggtggaac attatctaaa taggtgaagt atgccaatta gtatttctaa
     3301 ccaaatgaaa ctagatcttg ccaaacttga gcaaaatgca atgctggact tgtacgaggt
     3361 tgatttgcgt aaattgcaag acaaaaacgg caatgctggt agtgtttacc gcttttattc
     3421 tgggctaaat gaactcaaaa caagtattgt ctggcaaggt cgaacttacg acccttaccc
     3481 catcgaggca acaggatttg agcgtaacag cagcggccca agcaatcgcc ctactcttac
     3541 gctatcaaat ctgtttggct tagttacggg tattgctaat cagtttgacg aatgtatcgg
     3601 tgcgattgtt cgccgtcatc aggtttacgc ccaatattta gatgcagtaa actttgctgg
     3661 aggtaatgcc aaagccgatc ctaatcagga aatcatcagc cattttgtga ttgagcaact
     3721 ttcaagcctc acacgagaga ctgctacttt tacgcttgcg ttgccgattg aaaccgataa
     3781 tgccaa
//
LOCUS       DANLXQ010000196         3775 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.199, whole genome shotgun sequence.
ACCESSION   DANLXQ010000196 DANLXQ010000000
VERSION     DANLXQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.199"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(45..215)
                     /gene="rpmG"
                     /locus_tag="PXD94_002438"
     CDS             complement(45..215)
                     /gene="rpmG"
                     /locus_tag="PXD94_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL6287244.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPVV
                     RKHVIYKEAKIK"
     gene            complement(227..463)
                     /gene="rpmB"
                     /locus_tag="PXD94_002439"
     CDS             complement(227..463)
                     /gene="rpmB"
                     /locus_tag="PXD94_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL6287245.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            complement(695..1432)
                     /gene="radC"
                     /locus_tag="PXD94_002440"
     CDS             complement(695..1432)
                     /gene="radC"
                     /locus_tag="PXD94_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL6287246.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDSELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            1582..2784
                     /gene="coaBC"
                     /locus_tag="PXD94_002441"
     CDS             1582..2784
                     /gene="coaBC"
                     /locus_tag="PXD94_002441"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL6287247.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRHLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            2784..3101
                     /locus_tag="PXD94_002442"
     CDS             2784..3101
                     /locus_tag="PXD94_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287248.1"
                     /translation="MKLLVSHFHQGEGKFPTFSAGTAVRLGSECVNYPNWFACEIDGW
                     KTFVPSHFISEDKLICDYNPTELVAAQGERVEFLELHYQWAIVKRENEIGWLPCEILR
                     VIG"
     gene            3098..3280
                     /locus_tag="PXD94_002443"
     CDS             3098..3280
                     /locus_tag="PXD94_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL6287249.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMVKDQNEKV"
     gene            3267..>3775
                     /locus_tag="PXD94_002444"
     CDS             3267..>3775
                     /locus_tag="PXD94_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL6287250.1"
                     /translation="MKKYNIVFSADKNYLPYLEIALKSLLAHHNHLSIFILSTGDISH
                     HWIETLRPYLENRYSTLRLGYLDSNNLANFNSNGYITNSTYLRYYIEELFEHSDSPYW
                     IYLDCDLVVNGNITDPFTNLDFSFGSIAAIADPYVNKLKNHPYIYKNYFNAGVFYLNA
                     KHFSKQPFI"
BASE COUNT         1149 a          764 c          745 g         1117 t
ORIGIN      
        1 atgaaaaacc cagcattcgc tgggttttaa atcttaaaac aaaattattt aatttttgct
       61 tctttgtaga taacgtgttt acgcacaact ggatcaaatt ttttgatttc cattttttca
      121 ggcatattac gtttgttttt atctgttgtg tagaagtgac cagtttctgc tgtagaaact
      181 aaacggattt tctcacgagc gcctttagct gccatttttt agctccttag attttttcgc
      241 cacgagcacg gatttcagct aatactgcat caatgccttt tttatcaata atacgcatac
      301 ctttcgcagt taaacgtaag gttacgaaac ggttttcgct ctcaacccag aaacggtgag
      361 tgtgaaggtt tggtaagaaa cgacgtttag tcgcattcat tgcgtgtgag cggttgttac
      421 caactgctgg acgcttgccg gttacttggc aaactcttga catttgaatt tctccaataa
      481 ttttaagctt aaaaatggtt cgggcgatct cacaaaaggc gttgctcaac caacccctcc
      541 gattttgatt gcctcgtcag gcattaaccc gaccacagtc tatgtaataa agactgccga
      601 ttataccgat ttcatttgtt atattcaagt agaatttata tgcgtatctt ttactgtttc
      661 aggagaaatg ttaaaggtta cataaagcaa gcggtcattt tctatcagaa atttgcaaaa
      721 atgaatgctc cttcatctct aatttctctt cctcgaacga aaaatagtcc cctttgccga
      781 caataatatg atccacaaag cgaatatcaa ccaattcgca cgccatttcg atctttttag
      841 ttaaacttct gtccgactca ctcggcagac aagaacctga ggggtgatta tgagccacaa
      901 taatcgccgc ggcattatat tttaacgctc ttttaataat ttctcggggg tgaacagcag
      961 tttgattaat ggtgccgaag aacatttttt ctttataaat caatcggttt tggttgtcca
     1021 aaaacagcac cataaaaact tcccgttctt cttcctccaa ctcggtttgg aaatacataa
     1081 tcgccatata aggggcttga atcatctcgc taaattccat ttgttgagcc agatagcgtt
     1141 tggtcatctc tttagaggct tgtaactgaa tgtatttggt tttgcctaaa ccgtaaattt
     1201 tgcaaaaatc atcaatattt gcattcagta attggcgtaa cgagccaaat gccatcaaca
     1261 cactctctgc caatgccagc acaggcacag ctttcgtacc agtccgtaaa aatatcgcta
     1321 ataattcgga atcggttaat gaggctgccc cctgtgttaa taatttctca cgcggcatca
     1381 ttgttggctc ttgagaatct tcatcaaaaa aatcgttcat tttttcctcc atcaatttag
     1441 ttaagcataa aagagccaac ctttgcagac aaagggattt attcaatttt tcgagcaaga
     1501 tcgcaaaaat agtgaagttg tgagcaactc cttattccgc taaaatatgc aaaataaatc
     1561 cacgctttaa ggatagtaaa gatgttacaa aacaaaaaaa ttttagtcgg catttccggt
     1621 gggattgcag cctacaaaac cattgaattt atccgccact taaaaaaggc aaatgcggaa
     1681 gtgcgtgtag tgctaacccc agcagcagaa gcctttgtaa cgcctttgac cttacaagcg
     1741 atttccggca atgcggtttc cacttcacta ctcgacccac aagccgaact ggcgatgggg
     1801 catattgagc tggcaaaatg ggcggattta atcgttatcg ccccggcaag tgccgatttt
     1861 attgctcgcc tgcgtatggg aatgggcaac gatttacttt ccactctctg ccttgcgact
     1921 gcaagcccga ttttgcttgc accggcaatg aatcaacaaa tgtacaaaca gattgcggtg
     1981 caagaaaatc tgtctgtttt agtcaatcgt ggcgttcagc tcatcggccc gaatagtggc
     2041 ttccaagcct gtggtgatgt gggggctggc agaatgtctg agcctgaaga aatttaccaa
     2101 gcggtgttgg ctcatttctc caacaatcaa gatttagagg gcttaaatgt aaccattacc
     2161 gccggaccga ctcgtgaggc gattgaccct gtgcgttata tcagcaacca cagctccggc
     2221 aaaatgggct acgcgattgc taatgccttt gccgaacgtg gagcaaaggt tacgttgatt
     2281 agcggtccgg tgaatttacc aacgccgaaa aatgtggaac gaattaatgt ggaatctgcg
     2341 gttgagatgg agcaacaagc ggtcaaattt gcccaaaaat ctgcgatttt tattggctgt
     2401 gcagcggttg ccgattaccg aatggcggca gtctctgacc aaaaaattaa gaaaaccgat
     2461 gccaatgatg aactcacgct aaaattggta aaaaaccccg atattatcgc taacgtcgcc
     2521 aatcttgccg aaaaccgccc ttttgtggtt ggttttgccg cagaaacgca aaatgtggcg
     2581 gagtacgcta aatcgaaact cgaacgcaaa aatctggata tgatttgtgc caatgatgtc
     2641 tcaggcggac aagtgttcgg gcaggatcaa aatgcgttgc atttgttctg gaaaaatggt
     2701 gaaaaaacgc ttcctctggc ggataaaagc aaattggcag aaagtttggt acaggaaatt
     2761 gtaaaacatt atcgagaaaa ataatgaaat tactggttag ccattttcat caaggcgaag
     2821 ggaaatttcc tacattctca gctggtactg ccgttcgatt agggagtgaa tgtgtaaact
     2881 acccaaactg gtttgcctgt gaaattgacg gttggaaaac ctttgtgcca agccatttta
     2941 ttagcgaaga taaattgata tgcgattata acccaacgga acttgttgca gcacaaggcg
     3001 agagagtaga attccttgaa ctacattacc aatgggcaat tgtcaaaaga gaaaacgaaa
     3061 ttggctggct accttgtgaa atcttaaggg taataggatg aaaattatgc aacttacgtc
     3121 aaatcagtat atcaacgaca ttaaacatat tcttttatct gctcgccaaa aggcgtatca
     3181 agccgtgaat tcggcaatgg tcgaagccta ttggaaaatc ggtgaacgaa ttgtgcaaga
     3241 agagaaaaat atggtcaaag atcaaaatga aaaagtataa tattgtattt tcagcagata
     3301 aaaactacct gccatattta gagattgcct taaaatcact attagctcat cataatcatt
     3361 tatctatttt tatattgagt actggcgata tttctcacca ctggatagaa acattacgcc
     3421 cctatttaga aaatagatat tccacattaa gacttggcta tttagattca aataatttag
     3481 caaattttaa ctccaacggc tatattacta attcaactta cctacgatat tatattgaag
     3541 aactatttga acacagcgat agcccatatt ggatttattt agattgcgat cttgtggtta
     3601 atggaaatat tacggatcca tttacaaacc tagatttttc ttttggaagt attgctgcaa
     3661 ttgctgatcc ttatgtaaac aaactaaaaa atcatcccta tatttacaaa aactatttca
     3721 atgctggtgt attttatcta aatgcaaagc attttagtaa gcaaccattt ataca
//
LOCUS       DANLXQ010000197         3764 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.200, whole genome shotgun sequence.
ACCESSION   DANLXQ010000197 DANLXQ010000000
VERSION     DANLXQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.200"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            53..1054
                     /gene="pdxA"
                     /locus_tag="PXD94_002445"
     CDS             53..1054
                     /gene="pdxA"
                     /locus_tag="PXD94_002445"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL6287251.1"
                     /translation="MKPILGITMGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKII
                     QRALPIVHSELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYI
                     EEAVNLAMGGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLN
                     VIHVTTHVQLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMF
                     GDEDQKEIVPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKL
                     LGFDTGVNVTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            1065..2063
                     /locus_tag="PXD94_002446"
     CDS             1065..2063
                     /locus_tag="PXD94_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL6287252.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVIGNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            2060..3286
                     /locus_tag="PXD94_002447"
     CDS             2060..3286
                     /locus_tag="PXD94_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL6287253.1"
                     /translation="MKETLLVIADDLTGANDTAVMFAESGFDTVLKTKVSALAQIHPD
                     KAQVISVSTDSRAIGEKAKELTQIAISNAIQNSIGQIYLKIDSTMRGSVKYQIEGAIK
                     AWAGLYPDVKAIICPAYPEMGRTIEAGHLYVNGVPVNETASGKDPICPVHSSSMQKLL
                     PDAQVIPCDTVDNLQASIENSEYKQIVIDAKTHEDLSMIAQVINHIGNKIIPVGSAGL
                     AQKLKNIQNQQQPERNLKLGRALIVVTSIHETSQLQVDEYISTLGGKSIVFNPSPSQL
                     INYSQSEHSLKLQLNALIRFSRDHVIIRANPAKVVNAKSANITETSKEISQHLADLSK
                     FCLDNEKFDSLILFGGDGAAALLDQMNVTEMNVQYAVVPGVPLCSIEKGNYSGITVMT
                     KSGGFGNNTLLSNIMN"
     gene            3394..>3764
                     /locus_tag="PXD94_002448"
     CDS             3394..>3764
                     /locus_tag="PXD94_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL6287254.1"
                     /translation="MSKNRLEQILLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDE
                     EGVVIRTHGGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTT
                     TLCIAKKIAHMPLRVITTDLH"
BASE COUNT         1213 a          676 c          710 g         1165 t
ORIGIN      
        1 tcatataagc tatacttcgt tcaaaatgat taatttcaat caaggagtaa atatgaaacc
       61 tatattggga attacgatgg gagatgccgc cggtatcgga ccagaagtta ttattaaagc
      121 attagctaat caacggattt atgagttagc acaccctgtt gtgatcggtg atcgaaaaat
      181 tatacaacga gccttaccta ttgttcattc agaattgaaa ttaagaattc tcaataatct
      241 agataacttt gtagccgaat atggcgttat tgatatttta gatttgaata acctgcctga
      301 agatttgcct tttgctcaag ttgatccgcg tgctggaaaa gcagcttatg aatatattga
      361 agaggcagtt aatttagcaa tgggaggaaa aatccacgct attgttacag caccactgaa
      421 taaagaagcc ctacacgcag gtggtaaacc atttcctggc catacagaga tccttgcaac
      481 cctcagtaat actaaagatt actcaatgat gctaaccagt gaaaaactca atgttattca
      541 cgttactact cacgtccaac ttcgtaaagc ctgtgattta attaccaaag aacgagttgc
      601 tacagttatt gatttagcag aagaaaatgc tcgcattctt gggtttaaag aacctcgtat
      661 tgcagtcgct ggtttaaatc ctcattcagg tgaaaatgga atgtttggcg atgaagatca
      721 aaaagaaatt gttcccgcaa ttcaagaggc tcaagccaaa gggtttaatg ttacagggcc
      781 tgtccctccg gatactttat ttcaccgtgc agctaaccta aatgaattcg atattgttgt
      841 tgtgatgtat cacgatcaag ggcatatccc tatcaaatta ttaggctttg atacaggtgt
      901 aaacgttact gtcggtcttc catttattcg aacatctgtc gatcacggaa ctgctttccc
      961 aatagcaggc aaaggaacag cagatagtcg tagtatgact gaagcactct atttgggtgc
     1021 aaaaatggca aatatcaaat atgcgacaca ctaaggagat taatatgtct actcaaacat
     1081 ctaaatccat cgtcccgata tatgatggta tgaataaaat tcctggcggt gcaatggtaa
     1141 taccgctaat tcttggttct attattggta cattctttcc tgaattttta aatttaggtt
     1201 catttacgac ggctttattc aaatcatcag caggaccact tattgcacta ttaattttcg
     1261 ctacaggtat gcaaattacc gtgagaagtt ctggacctgt acttgcccat accggtgttg
     1321 tattactatt taaaactatt attcccgctc ttttagttgt gattttagga ttatgggtag
     1381 gtaatgatgg tgttatgggc gtttctttat tagctatgct aactgttgtt gcaaattcaa
     1441 atggtggtat ttggttagcg tttacaggga aatatggtga ttatagagat aaaggagctt
     1501 atgtcgcttc ggctgttaat gatggacctt tctttgtttt actattttta ggtgtatcag
     1561 gattagcaga tattcctctt tcattaatgc ttgcagctgt tattcctctt attattggta
     1621 tggttattgg taacttagat cctaaatgga cagagttaat gaaaccaaca ggtgcaatag
     1681 tcattccttt ctttgctttt gcattaggaa caggaatcaa tctgcaatct attattactg
     1741 gaggagcaac aggaattatt ttaggtatcg tagcttctct ctttacaggt tttcttgtat
     1801 ttatcggtta caaaacaatt ttaagacgtg gtaatcaatc aggaatcggg tttgcagcag
     1861 gaactactgc tggaaatgcc attattacac ctgaaattgt tgctcaagca gaccctacat
     1921 tagcaccttt tgttcaaact gcgactgcac agattgccgc agctgtatta gtttcagcaa
     1981 tcttagctcc attatttgca gcgtgggttt taaaacgtca aggtggctta gaacaagaca
     2041 ctataaaagg agaaaatcta tgaaagagac actcttagtt attgctgatg accttactgg
     2101 tgcaaatgat accgcggtta tgtttgctga atcaggattt gacactgtct taaaaactaa
     2161 agtttcggca ttagcccaaa ttcatcctga taaagcccaa gtaatttctg tttctacaga
     2221 ttcaagagca attggagaga aagcaaaaga gctgactcaa attgcaatta gcaatgctat
     2281 tcaaaatagt atcgggcaaa tttaccttaa aattgattcc acaatgagag gctctgttaa
     2341 atatcagatt gaaggtgcta ttaaagcttg ggctggttta tatcccgatg ttaaagcaat
     2401 tatttgtcct gcatatccag aaatgggaag aactatagaa gctggtcatt tatatgttaa
     2461 tggtgttcct gtaaatgaaa ctgcttcagg aaaagatcct atctgccctg tgcattcatc
     2521 ttctatgcaa aaactattgc ctgatgctca agtaatcccg tgtgatacgg tcgataattt
     2581 gcaggcatct attgaaaata gtgaatataa acagatcgtt attgatgcta aaacgcacga
     2641 agatctctca atgattgccc aagtcataaa tcacataggt aataaaatta ttcctgttgg
     2701 ctcagccgga ttggcacaga agttgaaaaa tatccaaaat caacaacaac cagagcgaaa
     2761 tcttaaatta ggtagagcat taattgtcgt aacctcaatt cacgaaacaa gccaactgca
     2821 agttgatgaa tatatttcaa ctttaggagg aaaatctatt gtatttaatc cttcgccttc
     2881 acagttgatt aactactcgc aatcagaaca ttcactgaaa ctacaattaa acgctctaat
     2941 acgttttagt agagatcatg ttattattcg ggcaaaccct gcaaaagttg taaatgcaaa
     3001 atctgccaat atcacggaaa cttctaaaga aatttctcaa catttagctg atttaagtaa
     3061 attctgcctt gataatgaaa aatttgatag ccttatatta tttggtggag atggtgcagc
     3121 agcattatta gatcaaatga atgtaacaga gatgaatgta caatatgcgg tagtaccggg
     3181 cgttccttta tgctccattg aaaagggcaa ctactcaggc atcactgtaa tgactaaatc
     3241 tggaggattt ggaaacaaca cactcttaag caatataatg aactagtaac aacaaagaac
     3301 ctaaagcaac aatgttttag gttctttaat tttaactatc ttatttttaa aaaattactt
     3361 atagtaaaag cagttaaaaa ttagagagtt tatatgtcta aaaaccgtct agaacaaatt
     3421 ttactattcc taaaaaacca caatattgct accgtagagc aactggtaaa agtgactgag
     3481 gcttctcctg caactatccg ccgtgattta ataaaattag atgaagaagg cgtcgtgatt
     3541 cgcacacacg gaggggtcag cttaaaccaa tttataccgt ttcaaccaac aacaaatgag
     3601 aagcaattta agcaaattgt cgaaaaagaa cgcatagctg aatatgccgt ttcgcttatt
     3661 tcaccgggaa attctatttt attagatgct ggcactacaa cgctatgcat tgcgaaaaaa
     3721 atcgctcata tgccacttcg agtgattaca actgatctac atat
//
LOCUS       DANLXQ010000198         3745 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.201, whole genome shotgun sequence.
ACCESSION   DANLXQ010000198 DANLXQ010000000
VERSION     DANLXQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.201"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1213)
                     /locus_tag="PXD94_002449"
     CDS             complement(<1..1213)
                     /locus_tag="PXD94_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL6287255.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQIDINIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLAMLELVTEHLRSRK
                     EWVKLDRLRVCFYAKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLLQNRQHWKIKQV
                     MNHENMLIDHLL"
     gene            1474..2691
                     /gene="fabB"
                     /locus_tag="PXD94_002450"
     CDS             1474..2691
                     /gene="fabB"
                     /locus_tag="PXD94_002450"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL6287256.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDVVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
     gene            2809..2955
                     /locus_tag="PXD94_002451"
     CDS             2809..2955
                     /locus_tag="PXD94_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287257.1"
                     /translation="MIKPLDSVYWRGKDVSEFFARKRLPDTAVQCVKDNELSYVYGYS
                     TSFV"
     gene            2924..3634
                     /locus_tag="PXD94_002452"
     CDS             2924..3634
                     /locus_tag="PXD94_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HDL6287258.1"
                     /translation="MFMVIQLALYNLQTYEVGRNATTIFMTTDKVYTGGSTWTYLFAD
                     ADNKITSVREMNGFGDVEKEFKCGEYKEAVTIASKEDLAKRKTQPRLWSSIYMSPEQN
                     MMWVTDNTSKNLENAKQDAFKQCEKNGGRNCQMILSFSNMCLAFAQGYKDGQGFEDFG
                     YGIVSSLAEENALENCNQKGGSSCLLSAPAVCAIPCDILSNNKCMYDQPQIIIPGKNN
                     NQPINPPANGNGEHWLSN"
BASE COUNT         1151 a          610 c          813 g         1171 t
ORIGIN      
        1 gtagtaaatg atcaatcagc atattctcat gattcatcac ttgcttaatt ttccaatgtt
       61 gacggttttg aagtaattga atttgttttt ctgaccaaga ccattggtta accagctcgt
      121 ctaaatcacg acttctccag ttgttattta tttctccctc aaccgctttt gcataaaaac
      181 agactcgcaa gcggtctaat ttcacccact ctttgcgtga tcttaagtgt tctgttacca
      241 attcaagcat ggctaagtag ggatcaaagt gatattccaa agcattgtca cttagtagtt
      301 ttttcttaaa ttctttggag atgagctttg ttttaggata agtgtgagcg tagcattcga
      361 gcaataaaat tttaattgcc gatttataag ggttattaat tcctttgtag agctgccata
      421 agcttgcacc aaaaaattca ctggttgaaa ggctggaaaa atcgccgaaa tcaatccatt
      481 ccgttaagtc aatttcaggg ttattgtgaa cattttggta ttcattttta tgtaaatgca
      541 accagagtaa acgcttaccg gcaagtaaaa tagctgaacg ataaaattca tctaatagaa
      601 aaaaatgctg tgctgaacca ctgtgttcat cggttacgct actgttataa gtttggctct
      661 taaattgttc tgggttcatt aaataaatat tgatgtcgat ttggaatgtc tttgcccatt
      721 cttgcaattt attaattttg agctccatta acttatattg ttcaattgta aaatggttcg
      781 gatagcatag ccataaatct aaatcagaac agcttgtttg ggtaatagta ccggtgctac
      841 ccatcgaata aagagcatca aagctgggat gggggcccaa atgatcggca gagattgctt
      901 catcaaaacg gtaagaaacc agaaattgtt tttgataatc agagagttga aaatgccaaa
      961 tcccttgtgg tgcattttca acataagccg gtaacacagg gtgattaaga tgaataagca
     1021 atggaatgag agagaaaaca tgcataaaat gatgattatt agcattgata gctcgttcta
     1081 ttcggtatgc attaagttga tcaattcgtg ctttagtaga gtttaatttt tgggcgaagc
     1141 tcccctggtt cgccccaatg gcagcgcctt ctacggctgt tattttgttg gctgtcaggg
     1201 ttaatgtttt cacgtcagct ttccttacta aagtgtgaac tgtttagcat tttagcggaa
     1261 aagtgattat tatgtaatag ggtaaaaaat cgggaaatta aatttcattc cctattttgc
     1321 aaaaaatgtc tgaaattcaa ccgctatctt gatagaaaga gttcagacca gtaatgaaat
     1381 tgtgtaaaat aatcaagttt tagtagctta tttgagtgaa caagtgtaag ctgaatcaag
     1441 tatttttcag atcaaaatga tgaaggaaac aaaatgaaaa gagtcgttat tacaggatta
     1501 ggtgtaattt ccagtatcgg taacaacaaa gaagaagtgt tggaatcact aaaagcaggt
     1561 aaatcaggca ttgaatttgt gcctgagttt gctgaagtgg gtatgcgtag ccaagtggca
     1621 ggtacgatta aattaaatcc ggctgagtta attgaccgta aaatctatcg ttttatggga
     1681 gatgctgcgg cttacgctta tctttcaatg aaagaagcaa tcgaagactc aggcttaacc
     1741 gaagaacaag tttcaaacga tcgtaccggt ttggtgatcg gagctggtac aggctcggcg
     1801 cacagtcaac ttgtagcttg tgatgcagta cgtggcccac gtggtgtgaa agccgttggg
     1861 ccttacgcag taaccaaaac aatggcatca agtgtgtcag catgcttggc aacaccttat
     1921 aaaattcgtg gtgtaaacta ttcaatcagc tcagcttgtg cgacttcagc acactgtatt
     1981 ggacacgcga tggagttaat ccaattaggt aaacaagatg tggtgtttgc aggtggtgca
     2041 gaagaacttt cttgggaatg tgcaaccgag tttgatgcaa tgggtgctgt ttcaactaaa
     2101 tataatgaaa caccaacaaa agctagccgt gcttatgatg ctaatcgtga cggttttgtg
     2161 attgccggtg gtggtgcagt ggttgtggtg gaagagttag aacacgctct tgctcgtggt
     2221 gccaaaatct atgctgaaat tgtgggctat ggggcaacct cagacggtta cgatatggtc
     2281 gcgccaagtg gtgaaggtgc tgagcgttgt atgagacagg cgatggcaac tgtaaatggt
     2341 gaaatcgagt atattaacgt acacggcact tctactccag tgggtgatgt aaaagagtta
     2401 ggggcgatcc gtaatgtctt tggtgataaa acgccagcaa tttcatcaac caaatcaatg
     2461 accggacact cattaggtgc agcaggtgcg cacgaggcaa tttattcatt attaatgtta
     2521 gataataatt ttattgcacc aagcattaat attgaaaccc ttgatgaaca agcacaaggt
     2581 atgaatattg tgactgaacg ccaagacaaa gcattaacaa ccgtgatgtc aaacagcttc
     2641 ggctttggtg gtactaatgc gtgtttagtg ttccaacgct attctaagta acataaaaag
     2701 agcaagtgaa agcttgctct tttttctttt gaggtaatta ttataaattt caaaataagt
     2761 tctttgctat ttgttatttt gtcattattt ttgaccgctt gttctacaat gattaagcca
     2821 ctagatagtg tttattggcg tggtaaagat gtatctgaat tttttgcacg taaacgttta
     2881 cctgatacag cggttcaatg cgtaaaagat aatgaattaa gctatgttta tggttattca
     2941 actagctttg tataatttgc aaacttatga agttggtcgt aatgcaacca ctatatttat
     3001 gacaacagat aaggtttata caggtggatc gacttggaca tatctttttg ctgacgctga
     3061 taataaaatc acttctgttc gtgaaatgaa tggttttggt gatgtcgaaa aagagtttaa
     3121 atgtggtgaa tataaagaag cggtaaccat tgccagtaaa gaggacttag caaagcgtaa
     3181 aacacagcct cgtttatggt catcaattta tatgtcacct gaacaaaata tgatgtgggt
     3241 gactgataat acgtctaaaa atttagaaaa tgcgaagcaa gatgcgttta aacagtgtga
     3301 aaagaacggt ggtcgtaatt gccaaatgat cttatcgttt agtaatatgt gccttgcttt
     3361 tgcacaaggt tataaagatg gtcaaggctt cgaggatttt gggtatggta tcgttagtag
     3421 tcttgctgaa gaaaatgcat tagagaattg taatcaaaaa ggtggttcaa gttgtcttct
     3481 ttctgcccct gccgtatgtg ctattccctg tgatatattg tcgaacaaca aatgtatgta
     3541 cgatcagcct caaattatta ttccaggtaa gaataataac caaccgatta atccaccagc
     3601 taatggcaac ggtgagcatt ggttgagtaa ttaaatcatt ttagtaaaaa gtgcggaaac
     3661 gcactttttt attgcctgca agcggtcatt tttagtgaaa actttgctaa aaatcttggt
     3721 aaataaaaat ttacaattta ttttt
//
LOCUS       DANLXQ010000199         3671 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.202, whole genome shotgun sequence.
ACCESSION   DANLXQ010000199 DANLXQ010000000
VERSION     DANLXQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.202"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(137..2701)
                     /locus_tag="PXD94_002453"
     CDS             complement(137..2701)
                     /locus_tag="PXD94_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL6287259.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKSLKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHIKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPGVLKSGELF"
     gene            2825..3523
                     /locus_tag="PXD94_002454"
     CDS             2825..3523
                     /locus_tag="PXD94_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL6287260.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            3499..>3671
                     /locus_tag="PXD94_002455"
     CDS             3499..>3671
                     /locus_tag="PXD94_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287261.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQ"
BASE COUNT         1115 a          815 c          608 g         1133 t
ORIGIN      
        1 tgcaataaaa ctcatttatt gactaaggaa tatccaaata taactcacaa atcgcccaaa
       61 ataaaattgg cacttattat cgcaagaaat ccccctattt ttcaactttt aagagcatta
      121 tccttgtaat aaattattag aataattcac cgctttttag cacccctggt ggaggtaatc
      181 ctaaacgtaa cggcgcagat tctgtttttt gaggcgcttt ttcagtataa gctctctcta
      241 caatataacg ttttgtaggc tcagtaccct taataaaata ctctttacgt ccatttccta
      301 agaaaccact attagggtca atattaacct caataatatt ttttggaagg gtactttttt
      361 cgacaggctt atctgacaat gcaactttca tataatttgt ccaagctggt aaagcagtac
      421 tagaacctga agcacctcta cctaatactc ttttattatc atcaaagcca acgtacacaa
      481 cagtagaaat attagcacca aatcccgcat accaagttgc cttggcatta ttggttgtac
      541 cagtcttccc gcccacatcc gctcgtttta tcgtattgag cattttatag cttgttcctc
      601 gccagccata ttcaggctca ccagtaattg ctgtagctaa agcactacgc attaaaaaag
      661 cgagttcatc actaataaca tgaggagcgt aacgtaaccc tttgatatga tgagaagatt
      721 ctgccattaa gctcggcgca tttttctcgc tattgttcgc ttgtaattca ggttctgaat
      781 cgccaacatc ttccacaaca tcgtcgtcat cattattcga ggaattcgct atagtttgta
      841 caccgacttg agcttcatca tgaattttga ctgattcaaa atatttaggc tcagggtaaa
      901 caactgggct atcgcaacta ttacaagcta ctacaggatt tgcttgatat aactcgtagc
      961 cttgggcatc aataatacgg tcaatgatat atggttcaat caaataacct ccattattaa
     1021 atactgcata agctcgagcc atttctaatg gcgtaaaaga tgcagcaccc aatgcaagag
     1081 attctgttgc aacatactga tttcggttaa agccaaaacg ttgtaagtag tctgcaacat
     1141 aatcaatccc tgccatttgt aatatacgaa tagccaccat atttttcgat ttacctaatg
     1201 caacacgagt tctcaacggg ccgccataaa ccccatcggc atttttaggt cgccactctt
     1261 tttgcccttt ctttttaatg acaataggag aatcgctaat tgttgttgaa agacttagcc
     1321 ctttattcat tgccgctgtg taaataaacg gtttaatcgc agagcctacc tgaactaatg
     1381 actgagtcgc acgattgaaa cggctttgtt caaagctaaa gcctccaacg atagattcaa
     1441 tggcaccgtt ttcactatta attgacacta gggctgaatt tacctccgga atttgtccta
     1501 aagcccactc cctattttta ttttgacgta cccaaatttg ttcacctgct tttaaccctt
     1561 taggtgcaaa agaagcattt gtacgtttta aaacagagat atcaccattt gccaataaaa
     1621 ttgtatattt tgatttatct gcttgcatta caacagctgg tgtaaacggc tctgattttg
     1681 gcagcttact taaatgatcg ataattttct catcgtccca agcagaacta cctgtccata
     1741 atttttcagc gcctcgccaa ccatgacggc ggtcataatc aatcaaattt tcacgtaacg
     1801 catcttgtgc tgctttttgg tctgctgaaa gtaccgtggc gtacactttt aagcctttgt
     1861 tataagcaat attttctcca tagcgtttca caatttcttg acgaaccatt tctgtcacat
     1921 aatccgctcg aaattctaat gtcgcaccat agtagttagc tactactggc tctgctttag
     1981 ctttctcata ttcctctttt gtaatatctc cggtttctag cattctccca agcactacat
     2041 tacgacggtc ttccgcccgt tttactgaat aaagtggatt catcgttgaa ggcgcttttg
     2101 gaagccctgc aataattgct atttcagaaa gggttaaatc tttcaaagat ttattaaaat
     2161 aagttttagc cgcagcggca acaccgtgag aacgataccc taggtaaatt ttgtttaaat
     2221 acagctccag aatctcattt ttactgagaa cctgttcaat ttctaaagct aaaattgcct
     2281 ctttggcctt acgctctaaa ctacgttcag gtgttaagaa aaagtttttc gccagctgct
     2341 gggtgattgt acttgcgcct tgagtatctc cctggctagt gcgccatatt gcacgcataa
     2401 tacctttagg atcgatccct ttatgctcgt aaaaacgtgc atcttcggta gcaagaatcg
     2461 cttttactaa catcggtgga atatcatcaa gcttgaccgg aatacggcgt tcttctccta
     2521 cttcaccaat taatttacca tctaaggtaa aaatctgcat aggctgctgt aactctacat
     2581 ttttcagcgt tgccacatca ggcaaatcag atttgaaatg catgtacagt aaaccaccaa
     2641 ttaccccacc taaaaccaag agtgaaagta aagcactaaa tattattttt gcgatccgca
     2701 tcgaaaaaat ctcttttggt taatgacgaa acataaattt aaagccaata ttaggtcact
     2761 aagtataagc taaaaacgct tacttcgcat aactaattgc aaaactttcc ttactatact
     2821 tactatgaaa ttacttaaag cacattcaaa aacaccttca aaccgtatca tcttaggctt
     2881 aagtgaagat gaacaacgtt actgcttagt caaaaataaa gcagaaaaat tcaccgtttt
     2941 ttggcaagcc aagcctgcga gcatcgaaga gcttatcggt aatctcaccc cacaagaaca
     3001 agcaatgatg acgctggttc gcccgatccc ttaccaatac atttggcgaa aaaccgtttt
     3061 tatggctaac accctcaatg aagctcaatt acaccgtaag gtaattcaca ttttaaaaaa
     3121 tgagcagcca cttgcattgg atctactcaa tattgactat cagcgatttc caagcagcaa
     3181 cgataatctg gataaagtgg ttatctacgc cgtgcgaaaa agttataccg agaatttgag
     3241 ccaatttaac agcatcttag attgtgagct gcattgctat gcacgggcta taattcattt
     3301 aacacaatcc caaaataatc agcctgcgcc ctgtttttca tttaaacaga agtacttcca
     3361 atttaccgag acggaattac tgatattgca aacagaacct gaggattgta ttcatctatc
     3421 tgatctcaaa attgaccatc ttgaaatcga gtgtgagcaa gagaaacagc tatattgcct
     3481 cgccttagga gcaagtttat ggaatggcaa ggtattgatt taagtgctaa tcgaaaaaat
     3541 aactggtttg ccggcagtga taaaggcatc aaaaacgtga tttatacact tttattatct
     3601 gccctcatta gcttggctct gatttttctc tatttgcaaa aaaatcaaga aattcaaccg
     3661 cttgcacagc a
//
LOCUS       DANLXQ010000200         3619 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.203, whole genome shotgun sequence.
ACCESSION   DANLXQ010000200 DANLXQ010000000
VERSION     DANLXQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.203"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            18..2093
                     /locus_tag="PXD94_002456"
                     /pseudo
     CDS             18..2093
                     /locus_tag="PXD94_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            2137..3573
                     /locus_tag="PXD94_002457"
     CDS             2137..3573
                     /locus_tag="PXD94_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL6287262.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQGKLIQKAQQPL
                     SLSIIFPDQSSRLLAQNVFGEQDFMFKSELIGTATFSILANGATVSDQDFTLTIERHI
                     PISAQIAPPKVIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQS
                     RVTFLWRGATKNARIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTL
                     PVDENTQRRALLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTE
                     APNIAKGKLQQLIFESATLGNKHRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKT
                     ILDNMIAQKVILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTK
                     ERIIGGSSYGGLSSAYTVLNYPNVFSKALILSGSFWWQPKGTPSEQSHYIAQQFIQKE
                     KLPLCFFISAGFYESGKSAICKRSNYFA"
BASE COUNT         1202 a          724 c          683 g         1010 t
ORIGIN      
        1 tatttaagga taaactaatg aaaataaaaa aatattcact ttctctaatc actttagcga
       61 ttgtagctcc tcaagtatta gcagaaacaa cctctcaagc agaactaaat gctattgagg
      121 tcaaagagaa attggcagct accaccggct atattcctgt ttatacgact gtaggaacga
      181 aaacattaac ctctttggct cgtacacctt ttagtatcaa tgttgtctct gatgatttaa
      241 tgcatgacca agctgttcgt tctgttaccg attcagtggc ttatacctcc ggtatgatgg
      301 gtggctatcg tggtaacaat tcgcttatcg aaatctccat tcgaggcatt ggcaataaaa
      361 gtgatggcag tacaattccg acctatttaa acggttctcg ctaccaaact gcatttgaaa
      421 tcgatccgtt cttcttagaa agtatcaatg tgattaaagg tccaaactcc attttatacg
      481 ggcaagctaa tcccggtggc gtaatggata ttatcaccaa aaaagccggc agtaacccac
      541 atcgccattt gcaatttgtg gtgggaaacc atcaacgata ccaaatcggg ctggatattg
      601 aagaaaatct ctccaatacc ttatccgctc gtttaatcgg tcaattagaa cgaacaaact
      661 ggaataccta atttgtaaaa gaaaaaggcg gtgcttttgc tccaagcatt ttatggaaac
      721 cgactacaaa tacagaactt aatgtttacg cttactatgc aaaaaaaccg aaagccggtg
      781 atcgtaactt tttaccgaaa gaaggaacga ttaatagcgt aaacgggaag gaactaccct
      841 atgatatgtt cctcagcgat ctgaactatc acggtttaaa agccgaacaa atgcaagtag
      901 gttacagttt ctcacataaa tttacgccaa acctaacgtt ccgccaaaat gcaagttacc
      961 ataatggcga agagaatttc aaaaatctag tttattgggg cgatgtcggt tcggttttag
     1021 aacgtagagc tcgccattgg gatgtcaaaa cccaagaaat caacttagat aaccaattag
     1081 aatgggtatt tagcacagca aatataaatc ataccttgtt agccggtatt gattataaac
     1141 gagtgcgtaa agatcttgat aactatatgg ggattgcccc tgattttgat tggcagaacc
     1201 ctgtttatgg cgtgaccgtt actgagccta gcttattttc aagtgaagtg aagcatttaa
     1261 aacaacttgg ggtttatcta caagatcaaa tagaaatcgg taattttgac ttcctgttag
     1321 gcggtcgtta cgatcgagca aaaaacagca accaagatcg tttgaacagt aaaacagaga
     1381 ataaccaaga aggaaaattt acttggcgta caggcgtagt ttacaacttt gaaaacggta
     1441 tcgctccgta tatcagctac agcacttctt ttgtgccatc ggctgaaaaa gatgctcaag
     1501 gaaatttctt aaaaccaaca actgctgggc aatgggaagt gggaatgaaa taccaaccta
     1561 cccaagacac cttactgacc ttagcaggat ttaaaattac acaaaaagat ttagccaatt
     1621 atcaatggca aacccgctct tatgagcaaa ttggtgaagt gcaaaccaaa ggaattgaag
     1681 tgagtttaaa tcagcaatta tccgataaat tttcaatagc ggcttcttat gcttacttaa
     1741 aaaaagagat tactgcagat aaagatggca cgacgattgg aaaaacgcag tggggagtgc
     1801 ctcgccatca agcatctctt tgggcaaaat ataaattctt tgataagctg accgccggtg
     1861 taggtgtacg ctatatggga acgacttttg gcgataacca aaatactttt accgtaccgg
     1921 catacacact ttacgatatg atgctagctt atgattttat gcctaactta aacttacagc
     1981 tcaacgcaca aaacctgacg aataaaaaat atgtcgcaag ctgtgccaat aatttttcct
     2041 gtttctatgg aaaagatcgc aatttaagtg caacgttaaa ctatcatttt taatctcaac
     2101 agccctcaaa tgagggctta aacttttgca aaaaatatga aaaaaacgac cgcttgtatt
     2161 cttacttttt tggtttcatc tgtgctttat gctaacaata taaccattga gcttaaacag
     2221 aatgattttg tgcaaggcaa attgattcag aaggctcaac agccactttc cttgtcgatt
     2281 atttttcctg accaaagctc tcgcctgctg gctcaaaatg tctttggtga acaagatttt
     2341 atgtttaaat cagaactaat cggcacagct actttttcta ttctagcgaa tggtgcaacc
     2401 gtttcagacc aagattttac cttaacgatt gagcggcata ttccaatctc ggcacaaatt
     2461 gctccaccta aagtaattga aaatacacag cttgaagaac tcagccaaaa aattgcaaaa
     2521 aatccagaaa aacagaccgc tttaattaac gatttttggg ataaagcaaa acaagccggc
     2581 acaccattga ttgaaccatt ggacgaacag caaagccgtg taactttttt atggcgtgga
     2641 gcaacgaaaa atgcccgtat ttggggcggt gtgagtactg aacacgattt tatgcaacgc
     2701 tttggtgaaa cggatatatg gtataaaagt tatatagtgc ccaatgacac tttagtggaa
     2761 tacaaattag ctccagatgt gccgacattg cccgttgatg agaataccca acgcagagcg
     2821 ttactggcta cggctcaagc cgatccactc aataaaacag catacttcga gaaaatcgga
     2881 caaaatattc ataataccga taaattcaac tacgcttcgc tacttaaatt gccaaacgca
     2941 cctactcaat cttttttgac tgaagctcca aatattgcaa aaggtaaact gcagcagttg
     3001 atttttgaga gtgcaacact cggcaataaa cacagattat tcgtctatct gcctgacggt
     3061 tttagtgcag aaaaaaacta tgcggttctc tatttatttg atggtgttga atataccgaa
     3121 cgagtgccaa ctaaaaccat tttagataat atgatcgctc agaaagtgat tctgcctact
     3181 gtagcggtgt ttatcgaaaa tccatcgagt gaatcaagac gtgtcgagct gcctgccaat
     3241 cccatattta cgcaagtatt ggcaaaagaa cttgtacctt ttgttgaaaa acagctcaat
     3301 ataaaaacaa aggagcgaat tattggcggc tctagctatg gcgggctatc ctctgcttac
     3361 accgtactca actatcctaa tgtatttagt aaagcattga ttctatccgg ttcattttgg
     3421 tggcaaccca aaggcacacc tagtgaacaa agccactata ttgctcaaca atttatccaa
     3481 aaagaaaaat taccactctg ttttttcatc agtgcaggtt tttatgaaag cgggaaatcg
     3541 gctatttgta agcggtcgaa ttattttgca tagaattaca ctcaattttg catagataaa
     3601 aattaaaaac tatgcaaaa
//
LOCUS       DANLXQ010000201         3523 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.204, whole genome shotgun sequence.
ACCESSION   DANLXQ010000201 DANLXQ010000000
VERSION     DANLXQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.204"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            13..375
                     /locus_tag="PXD94_002458"
     CDS             13..375
                     /locus_tag="PXD94_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL6287263.1"
                     /translation="MKPTNKADFQRVINAAGYSMKGLKAAYINEAAFRQEIWCATILF
                     PLGLILGETNIEKALLVGTVLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAA
                     VFMAMVLLAITWILILFC"
     gene            412..2055
                     /locus_tag="PXD94_002459"
     CDS             412..2055
                     /locus_tag="PXD94_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL6287264.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKATLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
     gene            complement(2262..3131)
                     /locus_tag="PXD94_002460"
     CDS             complement(2262..3131)
                     /locus_tag="PXD94_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL6287265.1"
                     /translation="MSSRGKILNTISSLQNSLTKTEKKIAAAILSQPELLSQCSLSEV
                     AKQLDVGEATFIRFCRTLGFKGYTDFKLELAIELATQNQDNRVLLDTDVSESDTSKDI
                     AEKLKVSLDNVIEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMR
                     IGLQTDAVTNNHFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITH
                     NLRSPVTEEANYVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQ
                     KTLNVILEQRVKG"
     gene            complement(3141..>3523)
                     /locus_tag="PXD94_002461"
     CDS             complement(3141..>3523)
                     /locus_tag="PXD94_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL6287266.1"
                     /translation="LDLVNLELGWQELENGIRMNVMSFETTPAEGKKAELHRKFIDIQ
                     LLIDGEEMIEYGLSQPDLSKYDEYRDEDDYQLTAEIDDKNNLILKPNMFAIFLPYEPH
                     KPGNSVNGNKTLKKLVVKVPVELI"
BASE COUNT         1130 a          682 c          638 g         1073 t
ORIGIN      
        1 attttattta ctatgaaacc aaccaataaa gcagattttc aacgtgtgat caatgcagct
       61 ggctactcta tgaaggggtt aaaagccgct tatattaatg aagccgcctt tcgccaagaa
      121 atttggtgcg ctactatttt attccctcta gggcttattc taggcgaaac caatattgaa
      181 aaagcattat tagtaggcac agttttactt gttttagtta cagaactact caatagtgcg
      241 gttgaatctg ttgttgaccg cattggggct gatttccacg agctttctgg tagagctaaa
      301 gatattggct ctgcagctgt atttatggcg atggttttac tcgctataac ttggatatta
      361 attttgtttt gttaactcta tttttcaacc aacactctta ctgaggtatt tatgcgctta
      421 aaaaaaacat tactttcggt agcactactt agtgcaacat cagcaatggc ctacgagaca
      481 ggtaaaacat atcgttttac ggttttacat actaacgata tacacggtca cttttggcaa
      541 agtgataaag gcgaatacgg tctcgctgct caaaaaacat tggttgataa tattcgcaaa
      601 gaggtcgcag aaaaaggtgg ttcggtatta atgctgaatg caggtgatgc gaatacaggc
      661 gtgccggagt cagatatgca aaatgcacgt cctgatattg aaggaatgaa tgcgattggt
      721 tacgatgcaa tggttctagg taatcacgaa tttgattacc cactgcaaat tctctctatg
      781 caagaaaaat gggctaaatt cccctttatt tcggccaatg tcattaataa aaaaacggat
      841 aaacccattg taaaacctta cgtgatgtta gaacgaggcg gactaaaaat tgctgttatc
      901 ggattaacaa ccgaagatac cgcaaaatta ggtaatccag atgtaacgga aaatgccttt
      961 ttcaaaaacc ctattgaaac agcaaaaaac acattagctg aaattaatca aaaagaaaaa
     1021 cccgacgtac gtatcgcttt aacccatatg ggttactatt ttgacggtaa acatggtact
     1081 aatgcaccag gtgatgtaac aatggcccgt accttagaaa aaggagcgtt cgatttaatc
     1141 attggcggtc atacccacga tactgtttgt attgatgaaa aaggtcaatt caaagccaaa
     1201 tatacgccgg gtgaggaatg taaaccagac ttccaaaacg gtacttggat tgtgcaagcc
     1261 ggcgaatggg gcaaatttgt tggacgggct gattttgagt ttaaagatgg aaaagctacc
     1321 ttggtaaaat atgaattaat tccggtcaac ttaaaaaaaa ccattaaaaa agaagacggt
     1381 actaaagaat accgacttta ccagcctgaa atcaaagccg atgaagcaac ctatacctta
     1441 ttgaaaaaat atcaagatga gggcgataaa aagcttaata ttgaagttgg tcgtgtgaaa
     1501 gggttattag acggcaaacg tgagcatatt cgcttccaac aaaccaatct cggtcgttta
     1561 attgcacaat cgcaaatgga aagggtaaaa gccgatatcg ggattatgaa ctccggcggt
     1621 attcgtacct cccttgaaga aggtgtaata acttataaac atattctgac tgttcaacct
     1681 ttcggtaata tgattgcaac ggtggattta aaaggacaag aattgttaga taccctcacc
     1741 actattgctc tgaaagaagt tgatactggc gcttatcctc aatttgccgg catttcaatg
     1801 gtagtcgacc gtacagcaaa aactatttct gatgttaaaa tcggtggcaa accttttgat
     1861 aaagagaaaa cttataaagt ttctctgcct gactatgttg cggctggtgg cgatggctac
     1921 ccattactga aaaaacaccc tagctatgtc aatacaggct ttattgatgc cgaaatgctg
     1981 aaaaaattct tcgaagaaaa taaaattatt gaggcagata aatacgatcc aaaaaaccag
     2041 atcattttca aatagagcta tagaaaatga aactcactct ggcagtctct gctcaaaaga
     2101 gctgtggctg cctgtagatt gttttcttac tttcaagtgt tataggaaat cgataaaaaa
     2161 tccctaccaa attaattcat caccatatga tgaaaattaa aaaacctctg gcaatacttt
     2221 gccaaaagtc tttgactttt atagggtaat atcactgctt attagccttt aacacgttgt
     2281 tccaaaatca cattcagtgt tttttgtttc tgtttcaacg cattttcggg ttcggcttta
     2341 actagtaagg tataaattaa atcaagaacg aaaagttgtg acattttagt accgatagaa
     2401 tctccttgca tatgcccctg tcgattacca ttaattaaca cataattagc ctcttccgtg
     2461 acgggggaac gtaaattgtg tgtaatcgca accgttgtcg ctttatttgc acgagaaaaa
     2521 cgtagtgatt tcacaatttc ttcagaatag cctgaatgac taatccctat aactaaatcc
     2581 ccctctttca ccaatgccgc ctgcatatac ataaaatgat tatttgtgac tgcatcggtt
     2641 tgtagcccaa tacgcattaa tttatgtttt gcatcttctg cggttaaccc agaagaaccc
     2701 actccaaata aaaagatccg ttttgcctta cgtaattctt caacaacttt ttccaacacg
     2761 ttaaagtcaa gcaaattaat agtctcttca ataacgttat ctaaactcac tttgagcttc
     2821 tcagcaatat ctttcgatgt atccgattct gaaacatccg tatcaagcaa tactctatta
     2881 tcttgatttt gcgtagccaa ctctattgca agctctaatt taaagtcggt ataaccttta
     2941 aaacctaatg tacgacaaaa acgaataaaa gttgcttcac caacatctaa ttgtttggcc
     3001 acttcagata atgaacattg gctaagtaat tccggctgag ataaaatagc agcagctatt
     3061 ttcttctctg ttttggttaa actattttgt agcgagctaa ttgtattaag aatttttccc
     3121 cgactagaca tagtagcctc ctaaatcaat tcaacaggta ctttcaccac cagttttttc
     3181 aatgttttgt tgccattcac cgaatttcca ggcttatgtg gttcataagg gaggaaaata
     3241 gcgaacatat ttggcttgag gatcaagtta tttttatcat caatctcagc tgttaattgg
     3301 tagtcatctt catctcgata ttcatcatac ttagataaat ctggctggct taacccgtat
     3361 tcaatcattt cttcgccgtc tatcaataat tgaatatcga taaattttcg gtgtaactct
     3421 gcttttttcc cctcagctgg cgtagtttca aatgacatta cattcatgcg aataccattt
     3481 tctaattctt gccagcctaa ttccaaatta actaaatcta aac
//
LOCUS       DANLXQ010000202         3511 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.205, whole genome shotgun sequence.
ACCESSION   DANLXQ010000202 DANLXQ010000000
VERSION     DANLXQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.205"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(5..127)
                     /locus_tag="PXD94_002462"
     CDS             complement(5..127)
                     /locus_tag="PXD94_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HDL6287267.1"
                     /translation="MKKLVLTILAAATFLTACNTVDGVGEDVEKAGDKIQQAAQ"
     gene            complement(207..1184)
                     /gene="rluD"
                     /locus_tag="PXD94_002463"
     CDS             complement(207..1184)
                     /gene="rluD"
                     /locus_tag="PXD94_002463"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL6287268.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            1325..2104
                     /locus_tag="PXD94_002464"
     CDS             1325..2104
                     /locus_tag="PXD94_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL6287269.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKLKYGEQF"
     gene            2177..3208
                     /gene="tsaD"
                     /locus_tag="PXD94_002465"
     CDS             2177..3208
                     /gene="tsaD"
                     /locus_tag="PXD94_002465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL6287270.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
     gene            3290..>3511
                     /locus_tag="PXD94_002466"
     CDS             3290..>3511
                     /locus_tag="PXD94_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL6287271.1"
                     /translation="MNNYVISLTSAQERRKHIEAEFGKQNIPFQFFDAITPDLIKEKA
                     KAFNIDISNTNLTKGEIACALSHIALWHLA"
BASE COUNT         1059 a          746 c          684 g         1022 t
ORIGIN      
        1 tttgttattg tgcagcctgc tgaattttat cgcccgcttt ttcaacatcc tcaccaacac
       61 catcaacggt attacacgca gtcaagaaag ttgcagctgc taaaatagtt aatactaatt
      121 ttttcataga aagctccttt tttgttgttt acttatgaaa acgatttcaa tatagcaact
      181 ttacattatt taagcaacac ctaaacttaa taatctaatt gatctttata aagctcataa
      241 tccgctttta gtgcctgaat caattcaata aaatcatcag gtaacggggc gtgccactcc
      301 atcatttcac cagtaatcgg atgctctaat ctcaacattg tggcgtgtaa ggcttggcgt
      361 tggaagcctc gtaacaccga taaaaacgag tcactcgcac ctttaggtgg gcgaggtcta
      421 ccgccataga gctgatcacc taataatggg tgagcaatat gcgccatatg tacccgaatt
      481 tggtgagtac gccctgtttc taatcttaaa cgtaaacgtg tgtaattacg aaaacgctcc
      541 ataatacgat aatgcgtaac cgccggtttt cccattgggt gaacagccat tgccgtacgt
      601 ttagttgggt ggcgagccat tggctcatca acttttccgc cctgtgtcat aatgccgctg
      661 gcaatcgctt catattcacg ggtaatacga cgtttttgta atgcggttac taagtgagtc
      721 tgtgccggaa tgtttttagc cactaccatt aagcctgtcg tgtctttatc caatcggtga
      781 acaatccctg ctcgaggcac ttctgcaatc ggtggataat aatgtagcaa agcattcagc
      841 actgtaccgt ccggattacc agcccctggg tgaaccacta aatccttcgg tttattaatg
      901 acgataatat cgtcatcttc atacacaata tttagaggaa tatcttgagg ctcaaaacga
      961 atttcctctt ctatttcagc ggtaatttca acagtttcac caccaaatac tttttccctt
     1021 gctttattca cgatattgcc attaactttc actagatcac tttcaatcca cactttaatg
     1081 cgtgaacgag aatagtccgg aaaaagttgt gccagagcct gatctaggcg agcacctagt
     1141 aaatctgcag taatttctgc tgttaaaatc ttttgttgag tcattaaacc ataaatccta
     1201 attagtcatt tgcttacttg cgtgatgtta tatagaatag cgagatgaac ttcataagtt
     1261 attcttagtc agaggacatt gtacattaac ttttcgtcct agacactttt tataggtaaa
     1321 acatatgcgt aaatttaaat ctcttgccac attagtatta gccggactgt ttgttgcagg
     1381 ttgttcaaat tcaaataaag aattagaaca atccaacgtt caagatcttt atggcaaagg
     1441 tcagacctat ttacaagatg gagattataa ttctgccatt cgctacttag aagcggttgg
     1501 aaccaaagga ggccaatatt caggacttgg tgagcaaacc caactaagtt taatttatgc
     1561 tcaatataaa gttggcgaat actataaagc acttgatgcc gcagaacgtt ttgctcgctc
     1621 ttacccgaat gcagctaata tggattacgt attctatcta gcaggattat caaatgcacg
     1681 tctaagtgat aattttattc aagacttctt tggtgtaaat cgcgcctctc gagccgtaga
     1741 taatgttcgt aatgcttacg gtaacttcca aactattgta cagcgctacc ctcaaagcca
     1801 atatgcacaa gatgctcaaa attggatgaa ttacttattt aaccgtttag cggaacacga
     1861 attagccgtt gttaaatttt atgatgaacg taatgcctat gtggctgtag taaaccgtat
     1921 cgaagaaatg ttgcgttttt acccgaatac actggcaacc aaacaagcat tgccatactt
     1981 aaaagaagca tataagcaaa tgaatatccc agatgcagag caaaaaacgg aactcttgat
     2041 ccaagaatat gaaagcaaag agctaccgaa tcctgaaaaa ctgaagtatg gcgaacaatt
     2101 ctagtcgtac agagaataat gtgaggggcg ttcttcgccc cttttggttt tctaacttat
     2161 tttgacttct ccaactatgc gaattttagg tattgaaacc tcttgtgatg aaaccggtgt
     2221 tgccatttat gatgaagaca aaggcttagt ggcaaaccag ctttatagcc aaattgatat
     2281 gcacgccgat tacggtggcg tagtccctga actggcttct cgagaccata tccgtaaaac
     2341 gttgccacta attcaagaag ccttaaaaga ggccaatctg caaccctcgg atattgacgg
     2401 cattgcctat actgccggcc caggcttggt cggggcttta ttggtcggct caaccattgc
     2461 ccgttcgctg gcttatgctt ggaatgttcc ggcattgggc gttcaccata tggaagggca
     2521 tttacttgcc ccaatgttgg aagaaaatgc ccctgaattt ccgtttgtgg cattattgat
     2581 ttcaggtgga cacacccaac tggtaaaagt tgacggcgtt gggcaatacg aactactcgg
     2641 ggaatcaatt gatgatgctg ccggtgaagc ctttgacaaa acaggcaaac tactcggttt
     2701 ggattaccct gccggtgtag cgatgtcaaa attagccgaa tccggcacgc caaatcgttt
     2761 taaattccct cgtccaatga ccgacagacc gggactggat ttcagtttct ccggtttaaa
     2821 aacctttgct gcgaatacga ttaaagccaa tcttaatgaa aatggtgaac tcgatgagca
     2881 aaccaaatgc gatattgccc acgcattcca acaagccgtg gttgatacta ttttaattaa
     2941 atgcaagcga gcgttagagc aaaccggcta taaacgctta gtaatggcag gcggcgtaag
     3001 tgccaataaa caattacgag cagaccttgc ggaaatgatg aaaaaattaa aaggcgaagt
     3061 attctaccct cgcccacaat tttgcactga caacggcgca atgattgcct acactggctt
     3121 tcttcgctta aaaaacgatg aacaaaccga cttaagcatt agcgtaaaac cccgctgggc
     3181 tatgaccgaa ttaccaccga ttaattaacc tttcaagcgg tgaaatttct tgttaatttt
     3241 gcaaaaattt aatcaaaaat aaccgcttgc tatatgatag attaaattta tgaataatta
     3301 tgtaattagc ctaacctccg cacaggagcg tagaaaacat attgaagctg aattcggcaa
     3361 acaaaatatt ccttttcaat ttttcgatgc gattacgccg gatttaatta aagaaaaagc
     3421 caaagcattt aatattgaca tttcaaatac aaacttaaca aaaggtgaaa tagcctgtgc
     3481 tttgagtcat attgcattat ggcatttagc c
//
LOCUS       DANLXQ010000203         3483 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.206, whole genome shotgun sequence.
ACCESSION   DANLXQ010000203 DANLXQ010000000
VERSION     DANLXQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.206"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            3..635
                     /gene="sspA"
                     /locus_tag="PXD94_002467"
     CDS             3..635
                     /gene="sspA"
                     /locus_tag="PXD94_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDL6287272.1"
                     /translation="MNKRSVMTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLGSISE
                     DLLELNPYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLNMYRI
                     EQDWYSQIDIVNKDPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDCYIAP
                     LLWRMQILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            635..1042
                     /locus_tag="PXD94_002468"
     CDS             635..1042
                     /locus_tag="PXD94_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL6287273.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            1239..2198
                     /locus_tag="PXD94_002469"
     CDS             1239..2198
                     /locus_tag="PXD94_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6287274.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGITIPEVLIKEAKQTF"
     gene            2278..2538
                     /locus_tag="PXD94_002470"
     CDS             2278..2538
                     /locus_tag="PXD94_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL6287275.1"
                     /translation="MGWIAAIIVGAIIGWIASAVMKSEGGLCKNIIIGILGSSLGRWV
                     FGDVLGIGSAYSAGDFNIVGIFFGVLGAALLIFILRKLSIIN"
     gene            complement(2588..3478)
                     /locus_tag="PXD94_002471"
     CDS             complement(2588..3478)
                     /locus_tag="PXD94_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6287276.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERDCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
BASE COUNT         1052 a          583 c          741 g         1107 t
ORIGIN      
        1 gtataaacaa acgttcggta atgactcttt tttcggataa aaatgatatt tacagccacc
       61 aagtgcgtat tgtgttagca gaaaaagggg tgccttatga aatagagaac gttatgctag
      121 gcagtatttc tgaagattta ttagaattga atccttacgg taatattcct acattggttg
      181 atcgtgaatt agtgctgttt aaccctcgta ttattatgga atatttagat gagcgtttcc
      241 ctcacccacc attaatgccg gtttaccctg tttctcgtgg tcagtgccgt ttaaatatgt
      301 atcgtattga acaagattgg tattcgcaaa ttgatattgt gaataaagat cctgaatcag
      361 cggaaggcaa gaaagcgtta gcacagctta aagaagatgt attggcatta gcgcctattt
      421 ttgccgctaa accttatttt atgagcgatg atttcagctt ggttgattgc tatattgcac
      481 cgttattatg gcgtatgcaa atcttaggcg ttaaattcag cggtgcaacg gcaaaaccaa
      541 ttaatgcgta tatgacccgt gtattccaac gtgatagttt tatccagtct gttggcggcg
      601 aagcgccaaa acatttaatg gacgataaag actaatgaaa ccattaagac cttatcttta
      661 taatgcctac tataactgga ttattgatag cgataacacg ccttacttat tagtgaatgc
      721 agaatttcca gacgtagatg tgccggtaga gtttgtcaaa gaagggaaga ttattttaaa
      781 tatttcgcct cgttccatcg ggcagtatat tgtggctgat gaatacatta gctttagcgc
      841 tcgttttcag ggaatgttaa gagatattta tattcctttt ggggcaatgg tggcgattta
      901 tgcgcaagaa accggtgatg gtattatgtt ccaagaagaa gcctactaca ctgaagaggc
      961 ttatcaagct cgagttagag cagtagagca gcctaaaaaa atcaaggcga agaaaacatc
     1021 taccttaaag ttggtgaaat aagagatcta tttcatcaaa acacattaca agcggttaaa
     1081 tttttctgat tatttgcaaa tatcagtcaa aatttaaccg cttgtttgtt ttgtgacgga
     1141 aataatcaaa ttccacttgc actccgacta aaaattgctt atctttgttt tgtgtttgta
     1201 ctgttgtgat agtacagtaa tgatcttaag gagagaaaat gaaattccat aaaaccttat
     1261 tagtgacttt gttaagttca ctttctgtag cggcattagc tcaagataaa attcaaaatg
     1321 tagcaattac agcgattgtg gaacaccctg cattagattc aattcgtaaa ggtgtgattg
     1381 aagaattagc ccgagaaggg tttgttgatg gtaaaaacat taaaatcgac tatcagtctg
     1441 cacagggtag tactgcaaca gcagcacaaa ttgcgcgtaa atttgttggg gataaagcag
     1501 atattattat cccgatcacc acaccttcgg cacaacctgt tgttgctgca acacgttcta
     1561 tcccaattgt gttttcaggc gtgacagacc cggttgctgc aaaattagta aaatcgtggg
     1621 agccaagtgg cactaacgtg acggggattt cagaccacaa agcgatggct ccacaagtga
     1681 aattaattca aacattagta ccggatttga aagctgttgg ttatgtgtat agtgcaggtg
     1741 aagttaattc tgcgattgtg ttagaagagt taaaacaaga ggctcaaaaa cagggcttta
     1801 ctatagtgcc ggttgctgtt caacgcagtg ccgatattgg cactgctgct cgcagcctaa
     1861 acggaaaagt gcaggctatt tatatttctg aagataatgc agttgtttcg gcttatgaag
     1921 ccttacataa agcggcttta gaagcaaaaa ttccggtgat tgctgctgac cgtgatacag
     1981 tacaacgtgg tgcagtggca gcttatgcgg ttaatcaata tgatattggt gtggcaaccg
     2041 gtaaagttgc tgctcgtgta ttaaaagggg aaaaagcagg tacaattcca atccaagaag
     2101 tcagcaaatt agagctttca attaatacta aaacggcaaa agaattgggc attactatac
     2161 cagaggtatt aatcaaagaa gctaaacaaa ctttttagtg taaaaaataa aaatttgttt
     2221 tatttgtcat aatcttgtag agtaaaaatt catttcaact cagctaagga gaatattatg
     2281 ggatggattg ctgcaattat tgttggcgct ataattggtt ggatagcttc ggctgtaatg
     2341 aaaagtgaag gcggactttg caaaaatatt attattggta ttttgggttc gtcacttgga
     2401 cgctgggtat ttggtgatgt attaggtatt ggttccgctt atagtgccgg agactttaat
     2461 atagtcggta tcttttttgg tgtattaggg gcagctcttt tgattttcat tttgagaaaa
     2521 ttaagcataa ttaactaatt tcgtatttca gaaaggaagc agttgtttta taactgcttc
     2581 ttttttgtta aatatactct ttttgctctt gcatcagttg tgtgaaaaaa tcaattagga
     2641 ttcgcacttt aggagagaga taacgacggt ttggatagag taagctgatt tgtaaaggtt
     2701 cctgtgtcaa aaaaggcata ataggaatca actctccgtt ttttagctcg tgttttatta
     2761 aaaactttgg aatgttaatg atgcccaaac cagcttttgc catttgtact aacgcataac
     2821 cattgttact gtaaatttta ccttgtggtt taatttgcat tttttgtcct tgttgagaaa
     2881 attgccattg aggatggtat tcatagagta ggcagtctcg ttccactaat gcttctaagc
     2941 ctttaggttc gccaaatcga gctaaataat ctggagaagc aactaaaacc aaatgggaat
     3001 ctgcaatttt tttcgctaca atggaactat ctgcaaggct accaatacgc agcaccaaat
     3061 caaagccttc tgcaatcaca tctacccgac ggtcactaaa ttcaatatga agggctaaat
     3121 tagggtaatc ttccataaat ttcccaaggt aaggggcaat gaagcgagag ccaaaatcca
     3181 ttggtacaga cattgttatt ttcccttgta gcgttgagtt aatattactt acgctggcat
     3241 cggcctcttt tagttcattt aaaataggca agcattgctg atagtagagc aaccctgcct
     3301 cagttggcgt aatttttcga gtggttcgtt gtagtaaacg cacattgaga tgttcttcta
     3361 actgagccac caatttactt gccatgggta acgaaatttg agcttgttgt gctgcttgag
     3421 tgaaactttg ggtttcaatt actttacaaa ataaactgat tgcgtttagt ttatccattt
     3481 tct
//
LOCUS       DANLXQ010000204         3418 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.208, whole genome shotgun sequence.
ACCESSION   DANLXQ010000204 DANLXQ010000000
VERSION     DANLXQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.208"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1467)
                     /locus_tag="PXD94_002472"
     CDS             complement(<1..1467)
                     /locus_tag="PXD94_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL6287277.1"
                     /translation="MTLQIHSIRTKVKTPLAKRIAHALTDRKILQFRFDAHLRNQVWQ
                     RLTKTQKALLNRINAAGIDALPKRELDKLLKELKAEIAKNYQETTAYIQPELSGFFAV
                     EKQALTELYNDEIGYDFFNQVPSYQRKATQQATIIAGMPLESWFEKQGSELSFKFEST
                     IRQGLLEGRQTPEIAKATAELFGISRRHADTLVITAVAKVADDAHKALRDENLDLIKG
                     EMHLSTLDTRTSTVCQVRDGKMWDTDYKPIGHSLPYQRPPLHPRCRSILQLVMKDWKE
                     LGFDDVKEMPKSTRASADGQVSEKTNYEEWLSSKTPEEQDKALGKGKADLWRRGVITF
                     SDMLDQSARPLTLKELYSLKNSSSVDKMDSVELVRKKAVAVEPMITRDVVSIITASGG
                     EPAGLDFRLKSLSSLQRKIDTEIMAGVSKEQAIASIRDVIRYTAILDEQRFVEQYQKM
                     QKDLEKQGYSTIIVKNTWKSNNAYKGINTFVSTFIEKNN"
     gene            complement(1415..2818)
                     /locus_tag="PXD94_002473"
     CDS             complement(1415..2818)
                     /locus_tag="PXD94_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HDL6287278.1"
                     /translation="MPNSISADFAALKAKTKIIDDLLGGTQKMRQAGQTYLYKMKMEE
                     EDGYKNRLNRSTLYPALRETLSQMLGRVFFNPINVEDVHKQIEPLFDDIDLEGNNLDV
                     FASRWFYAALAYGVSYCLVDFVRVENARSLAEEKAQNARPYLVHIKPQQVLGFKTAKI
                     NGKTAFTQFRYKETVTEDDGEFASKQVNYIYVYEIGRVRKYKNDEGGLSLVADMQIKA
                     QNTPLAFVPIVPFITKPIDVWGMGEPPLMELAYLNIKHWQSQSDQDNILNIARVPLLA
                     IFSDTQIKSLEIGSSAISLPKDSTMQFIEHSGSSINAGLTSLKELEDQMKTAGAKLLT
                     KTALAMTDSQAKDEAGKEISQLRHYANKFEDALDLAFEYIGHWLGIGVDEVGTSQISG
                     NIDNDLDPNASMATIIQLRNAGVISNHSTFEEAKRRGLLSDSAKWEDEQARLQAEGLS
                     DDFTNPFNSNQSENAAS"
     gene            complement(2831..>3418)
                     /locus_tag="PXD94_002474"
     CDS             complement(2831..>3418)
                     /locus_tag="PXD94_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL6287279.1"
                     /translation="GDYLESSDAQIFRDKYQELEFKPNPDFNGPYYGLDFGFANDPTA
                     CVKCWVFDDDLYIEREAGKVRLELDETAAYLANALPDLSNYTVRADSARPESISYLKR
                     HGLPYIQPVPKWKGSVEDGVEHIKSYRKVYIHPSCTEVLKEFRLYSYKTDRLSGDVLP
                     IIIDAHNHYIDAIRYALNPLIQARGEAKQCALKVY"
BASE COUNT          925 a          773 c          621 g         1099 t
ORIGIN      
        1 attattcttt tcaataaaag tgctcacaaa cgtattaatg cccttataag cattatttga
       61 tttccaagtg tttttaacaa tgatcgtaga ataaccttgt ttctccaagt ctttttgcat
      121 tttttggtat tgctcaacaa aacgctgttc atctaagatt gcagtgtagc gaataacatc
      181 tcgaatactt gctatcgctt gctcttttga cactccggcc attatttccg tatcaatttt
      241 tcgctgcaaa gatgatagtg atttcaagcg aaaatctaag ccggcaggct cgcccccaga
      301 tgctgtaata attgatacta catcacgagt aatcataggc tcaactgcaa cagccttttt
      361 acggacaagt tccacactat ccattttatc taccgaagaa ctatttttta atgaatatag
      421 ctcttttagt gtcaatggtc tagcactttg atctaacata tcgctaaacg ttatcactcc
      481 ccgccgccac aaatccgcct tgcctttgcc tagcgcttta tcttgctctt ccggtgtttt
      541 gctactgagc cactcttcat agtttgtctt ctccgacact tgcccgtctg cactcgccct
      601 agtgcttttc ggcatttctt tcacatcatc aaagcctaac tctttccagt ctttcatcac
      661 cagttgcaga atcgaacgac agcgagggtg aagtggtggg cgttgatacg gcaaactgtg
      721 tccgataggc ttgtaatcag tgtcccacat cttaccgtct cgcacttgac aaacagtact
      781 ggtgcgggta tcgagcgttg ataagtgcat ctcaccctta attaaatcaa gattttcatc
      841 tcgcaatgcc ttgtgagcat cgtccgccac ttttgccaca gcagtaatga caagtgtatc
      901 agcgtgccgt ctggatatac caaataattc agccgtagct ttggcaatct caggcgtttg
      961 cctaccctct aacaagcctt gccgaatggt gctttcaaat ttaaacgaaa gctctgatcc
     1021 ttgcttttca aaccaacttt caagcggcat tccggcaata atagtcgctt gttgcgtagc
     1081 tttacgttga tatgacggca cttgattgaa aaaatcatag ccgatttcat cattgtaaag
     1141 ctcagtcagt gcctgttttt caaccgcaaa aaaaccgctt aattcaggct gaatgtaagc
     1201 ggtcgtttct tggtaatttt ttgcaatttc tgctttgagt tctttgagca gtttatccaa
     1261 ctctcttttc ggtaaggcat ctatcccggc tgcattaatt cggtttaata atgccttttg
     1321 cgtttttgtg agtcgttgcc aaacttgatt tcgtaaatga gcatcaaaac ggaactgcaa
     1381 tattttacga tctgttaatg cgtgagcgat ccgtttagct agcggcgttt tcactttggt
     1441 tcgaattgaa tggatttgta aagtcatcgc ttaaaccttc cgcttgtaac cttgcttgtt
     1501 cgtcttccca ttttgcacta tctgacaata aacctcgtct tttagcttct tcaaacgtag
     1561 agtgatttga aatgacaccg gcattgcgta gctgaataat ggttgccatt gacgcatttg
     1621 gatctaaatc attgtcgata ttgcctgaaa tttgcgaagt cccaacttca tcaacgccga
     1681 tacctagcca atgcccaatg tactcaaaag ccaaatcgag agcatcttca aatttgttag
     1741 cataatgtct aagctgtgag atctctttac ctgcttcatc tttagcctga ctatccgtca
     1801 ttgctaatgc tgttttagtt agcagtttcg caccggcagt tttcatttgg tcttctagtt
     1861 ctttcagact ggttaaaccg gcattaatag acgaacctga atgctcaata aactgcattg
     1921 tactgtcttt cggcagagat attgcacttg agccgatttc aagggatttt atttgagtat
     1981 cagagaaaat agctaatagc ggtacacgag caatattcaa aatattgtct tgatctgact
     2041 gactttgcca gtgcttaata tttaaataag ccagctccat taaaggcggc tcacccattc
     2101 cccaaacatc aatcggttta gtgataaatg gaacaatagg cacaaatgca agcggtgtat
     2161 tttgagcttt gatttgcata tcagcaacca gtgataagcc accctcatcg ttcttatatt
     2221 ttcggacacg tccgatttca taaacataaa tgtagttcac ctgtttagaa gcaaactcgc
     2281 catcatcttc tgtaactgtc tctttataac gaaattgggt aaaagcagtt ttaccgttga
     2341 ttttggctgt tttaaagccc agcacttgtt gcggcttaat atgcaccaaa tacggacgtg
     2401 cattttgagc tttttcttct gcaagactgc gggcattctc taccctcaca aaatccacca
     2461 agcaataaga tacgccataa gccaatgcag cataaaacca acgagaagcg aatacatcaa
     2521 gattgttacc ctctaaatca atatcatcaa acaatggttc gatttgttta tgcacatcct
     2581 ctacgttgat cggattaaaa aacactctcc ccaacatctg cgaaagcgtt tccctcaatg
     2641 ccggataaag cgtagaacga tttaatcggt ttttatatcc atcttcctct tccattttca
     2701 ttttatagag gtaggtttga cccgcttgtc gcattttttg tgtgccaccg agtaaatcat
     2761 cgatgatttt tgtttttgct tttaatgccg caaaatcggc tgagatggag ttaggcattg
     2821 ttacctcatt ttaataaacc tttaatgcac attgcttcgc ctcgcctctt gcctgaataa
     2881 gaggatttaa ggcataacga atggcatcaa tatagtggtt atgagcgtca ataatgatag
     2941 gcagtacatc accacttaat ctgtccgttt tgtaactata aaggcgaaat tctttcaata
     3001 cctctgtaca acttggatga atatatactt tacgataaga ttttatatgc tcaaccccat
     3061 cttcgactga gcctttccac tttggaacag gttgaatata aggcaatcca tgtcgtttta
     3121 aataactgat ggattctggg cgagcagaat ctgctctaac agtataatta ctcaaatcag
     3181 gcaaagcatt tgctaaataa gcagcggtct catcgagctc tagtcttact ttccctgctt
     3241 cacgctcgat atataagtca tcatcgaaca cccaacattt cacacaggct gttgggtcat
     3301 tggcaaaacc aaagtccagc ccgtaataag gaccgttaaa atcagggtta ggcttaaatt
     3361 ctaattcttg gtatttatct ctaaagattt gagcatcaga ggattctaag tagtcccc
//
LOCUS       DANLXQ010000205         3389 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.209, whole genome shotgun sequence.
ACCESSION   DANLXQ010000205 DANLXQ010000000
VERSION     DANLXQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.209"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            410..928
                     /locus_tag="PXD94_002475"
     CDS             410..928
                     /locus_tag="PXD94_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6287280.1"
                     /translation="MRYIKYSLFLVSTMLISGCAELSAINDKVGDWAGQMNQTLYGSK
                     DGSVEQHNGRQVYTKTRNGKYVYQDSGTLQLKRNIDDVFLKVRREFGFRPTNADSDSR
                     KVYHTVQGTFYHVSGYFESDVDTDGVHLGENYLEVIIEKGSKNQVNVEWKATGSARWL
                     DSAKQRLLNTLK"
     gene            1088..3139
                     /locus_tag="PXD94_002476"
     CDS             1088..3139
                     /locus_tag="PXD94_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL6287281.1"
                     /translation="MKLFICEKPSQARDLAGVLGATSKGEGLLATADKSIIVTWGFGH
                     LVEQYQPEDYDEAWKRWAFETLPIIPAQWKMTPKKESKKQYSVVIGLIKKATSVVIAT
                     DADREGEMIARELLDLAGYRGQIQRCWLSALDDASIRKALQTLKSGKETEALYYAGMG
                     RSRSDWLIGMNFSRLFTLMAQAKGYSGKPLSVGRVQSPTLTLVVNRDREIANFVPKSH
                     YALAVQLTTTSGETFVAGLAVPEQYLDESGLCLDSRVIQQAEAQIKQTGLAKVVTVET
                     KREKKSPPLLYALSDLQGECNRLFGFGAQQVLDIAQSLYEEHKITTYPRTDCGYLPES
                     QFSDVPNVIRSLVASDSGLQTLLPKLNLRQKSRAWDDKKITAHHGIIPTIKKADLSKL
                     SEEEMKVYDLIRRRYLAQFLPHLEMDKTVATLQSSGHTLVARGNVIVAQGWRILFGKN
                     VDEETGNDDEKQGLPVLTKNQQCNVTHSEVRTLQTKPPAHYTEGTLLDAMKNAARFVT
                     DPRLKQRLRETEGLGTEATRAGLIQGLIDKGFLLKKKKSLVASAEAVALIDSLPDLLK
                     NPGLTALWEQALNQIAEGSMTLDAFMQKQEAFVKQLIGSCMQQGMSLGNIEIRKCSEC
                     GKPMRKINHAKGTFWGCTGYPDCQHKEADKKVKSASNKSAKKSSNLNVLDQLNKLRSQ
                     L"
     gene            complement(3200..3337)
                     /locus_tag="PXD94_002477"
     CDS             complement(3200..3337)
                     /locus_tag="PXD94_002477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287282.1"
                     /translation="MTLQSRFRQLQQTNVAGIIHLMFSWHSHKVEFPLRMLGDILAGV
                     D"
BASE COUNT         1066 a          659 c          721 g          943 t
ORIGIN      
        1 aaaaataact ttgatagagg ataaatatat gaatttccct ccaactcgat ggaaaatatt
       61 acaccagtat caaactgagc tagagctctg ggttgaacaa gccacagaag agcatatcaa
      121 gcaatacttg actgaaattc acggatgtgt atacccagat gactgggaaa atgtgcgtat
      181 atcccatatg tcatctcaaa ctcatatttt ccatagctgt gaacgttgcc tagaacttat
      241 ctctgcgata ttaacaatct cacaactaag cagacccgat ctaaatggtc tactgtgtga
      301 cagaaagcta taatattttt tctctttgta agtaaaagag ttttaagtag attttaaccg
      361 cacttttgca aaaaatgaac gcagataacc cgcttgtaag gagaattcta tgcgttacat
      421 caaatatagt ttatttcttg tttctacaat gctaatttca ggctgtgctg aactctcagc
      481 aattaacgat aaggttgggg attgggccgg gcaaatgaat cagacgctct atggctctaa
      541 agacggctct gttgaacagc ataatgggcg acaagtttat actaaaaccc gaaatggtaa
      601 gtacgtttat caagattccg gcacattgca gttgaagcgg aatattgatg atgtattcct
      661 gaaagttcgt cgggaatttg gcttccgccc aacgaatgct gatagtgact cccgtaaagt
      721 ttatcatacg gtacaaggta ctttttacca tgtttccggc tattttgaat ctgatgtaga
      781 tactgatggg gtacacttag gtgaaaacta cttggaagtt atcattgaaa aaggcagtaa
      841 aaatcaggtg aatgttgaat ggaaagcaac tggtagtgct agatggctcg atagtgcaaa
      901 acagcgttta cttaatacat taaaataata actcatattg ctcctagaac ctcttcccag
      961 tggaaacact gggatttttt atgtccttat ttctcaaatt attacatcct acgagacaat
     1021 aagtcgttga atgctattta atcagcacat tactatttgc ctatctgtat tcattttagg
     1081 aaataatatg aaattattta tttgtgaaaa gccatctcaa gctcgagatc ttgccggcgt
     1141 attgggtgct acttcaaaag gtgaaggctt gctggcgact gcggataaat ctattattgt
     1201 cacttggggg ttcgggcatc tcgttgagca atatcagccg gaagattatg atgaagcgtg
     1261 gaaacgctgg gcatttgaaa cattacccat tattcctgct cagtggaaaa tgacaccgaa
     1321 aaaagagtct aagaagcaat attctgttgt aatagggctt atcaaaaaag caacctccgt
     1381 ggtcattgcg accgatgctg atcgagaagg tgagatgatt gctcgtgagc tgttagattt
     1441 agctggatac cgaggtcaaa tccaacgttg ctggctatca gctctggatg atgccagtat
     1501 tcgaaaggct cttcaaacac tcaaatcagg taaagaaacc gaggctcttt actacgccgg
     1561 tatgggacgt agccgctcgg actggttgat tggaatgaac ttctcccgtt tatttacctt
     1621 aatggctcaa gcaaaaggct attccggtaa gccattgagc gttggtcgag tacaaagtcc
     1681 aacattaact cttgtggtta atcgagatag agaaattgct aatttcgtgc ctaaatctca
     1741 ttatgcgtta gcggttcagc tgacaacaac ctctggtgaa actttcgttg ccgggcttgc
     1801 agtacctgaa cagtatttgg atgagtccgg actttgcctt gatagtcgtg tcatacaaca
     1861 agccgaagct caaattaaac aaacaggctt agccaaagtg gtgacggttg aaactaaacg
     1921 agagaaaaaa tctccaccgt tactctacgc attaagtgat ttacaaggcg aatgtaaccg
     1981 cttgtttggc tttggggctc agcaggtgtt ggatatcgca caatcccttt acgaagagca
     2041 taaaatcacg acttatcccc gtacagactg tggttatctg ccagaatcac aattttctga
     2101 tgttcctaac gtaatccgct cattagttgc ctctgattca gggctacaaa ctctcttgcc
     2161 taaattaaat ttgcgacaaa aaagtcgggc gtgggacgat aaaaaaatta ccgctcacca
     2221 cggtattatc ccaaccatta aaaaagctga tttaagtaaa ttgagtgagg aggaaatgaa
     2281 ggtctatgat ctcattcgcc gccgatatct tgctcaattc ttaccgcact tggaaatgga
     2341 taaaaccgtt gccactctgc aatctagtgg gcacactctg gttgctcggg gaaatgtcat
     2401 cgttgctcaa ggctggcgta tcttgtttgg caaaaatgtg gatgaggaaa ctggtaatga
     2461 tgatgagaaa caaggattac ctgtattgac taaaaatcag caatgcaatg tcactcattc
     2521 tgaagtccga acattgcaaa ctaaacctcc cgcacactat acagagggga cattactcga
     2581 tgcaatgaaa aatgccgctc gctttgtgac tgacccaaga ctcaaacaac gcttacgtga
     2641 aacggaaggt ttaggcacag aagcaacccg tgccggatta attcaaggcc taattgacaa
     2701 aggatttctc cttaagaaga aaaaatcact ggttgcctca gcagaagccg ttgccttaat
     2761 tgatagtttg ccggacttac tgaaaaatcc cggtttaacg gcattatggg aacaagccct
     2821 caatcagatt gccgaaggta gtatgacact tgatgcattt atgcaaaagc aagaagcatt
     2881 tgtaaaacaa cttatcggca gttgtatgca acagggaatg tcacttggga atattgaaat
     2941 ccgaaaatgt tcagaatgtg gtaagccaat gagaaaaatc aatcacgcta agggaacatt
     3001 ctggggatgt actggttatc cagattgcca acataaagag gctgataaaa aggtaaaatc
     3061 agcttcaaat aaatctgcta agaaaagtag taatttaaat gtactcgatc aactgaataa
     3121 attaagaagc cagttataaa ttcaagtaga ttatggtatt atcaaggaac aaagaacccg
     3181 ttttgtagat tgtcggttat caatctacac cagccaaaat gtctcccagc attctgagag
     3241 ggaactccac tttgtgggaa tgccaactaa acattaaatg gataatgcca gccacattag
     3301 tctgttgtaa ttgtctaaat cgtgattgca acgtcataat attacccgat tggggggatt
     3361 atgacgttgc aattactgct aactattct
//
LOCUS       DANLXQ010000206         3386 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.210, whole genome shotgun sequence.
ACCESSION   DANLXQ010000206 DANLXQ010000000
VERSION     DANLXQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.210"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(105..728)
                     /locus_tag="PXD94_002478"
     CDS             complement(105..728)
                     /locus_tag="PXD94_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL6287283.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGRDFSAKMEALLENEEIESQANVEKPKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEIAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQKAQTSSNTESEPNIQK"
     gene            complement(774..1346)
                     /gene="rpoE"
                     /locus_tag="PXD94_002479"
     CDS             complement(774..1346)
                     /gene="rpoE"
                     /locus_tag="PXD94_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL6287284.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(1568..3112)
                     /gene="leuA"
                     /locus_tag="PXD94_002480"
     CDS             complement(1568..3112)
                     /gene="leuA"
                     /locus_tag="PXD94_002480"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL6287285.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
BASE COUNT          917 a          737 c          596 g         1136 t
ORIGIN      
        1 tttcgctact tgttggttat ttcttatttt ttcgctacat ttacatacaa aaagccacat
       61 aattatggat aaattatgtg gctttcagta acttatttta cctattattt ttgtatattt
      121 ggctctgatt ctgtattgga agaagtttgt gctttttgct tttcttcctc agataaggtt
      181 aaatttttaa cgttattagt acggcgttgt aactcatggt tttctaataa cgcattaatc
      241 ttacgctgtt gaagctctaa ctgttcttct gttggttgat cttgaactgg cgcattataa
      301 cttaccgctt caactgaatt gctaaatggc aatgtttgta atactggttg ttgcgtttga
      361 gctatttcat cactcgcatt cattgaatta acccctaata ccgcaactaa gcacactgaa
      421 gctgcaatac cggcttgcat aattggagtt ccccaacgct tgagcttgag caataagcct
      481 ttaggtttct ctacatttgc ttgagattca atttcttcat tttccagtaa tgcttccatt
      541 ttagcagaga aatctctccc taaaatgatc tcatcacctt gcatcacact acgaatagtg
      601 tgataacttg cccatttttg ctgtaattca gggctgttag ttaatgtttc tacaaatgct
      661 gtatcaacat tttgcccatc catataagct gaaaggcgtt cattttgttg agtgatgcgt
      721 tcttgcataa ttgactccaa tgatttaaat ctcttctaaa ataatctttc tatctattgc
      781 ataagtggct tgattttagc atcaatcgct tcccttgctc ggaaaatacg ggaacgtaca
      841 gtaccaaccg gacattccat cacttctgcg atttcttcgt aacttagccc ttccaattct
      901 cttaaacgaa tggcggtttt aagctcatca ggtaaatttt caatcgtatc aaacacgact
      961 ttctttaatt cctcggataa tatcaaattc tctggcgtat cactttcacg aagttgcgcg
     1021 cctacatcat aactttcagc gtcttcggca agaatatctt cttttggcgg tcttctgcct
     1081 aaagaggtta aatgattttt cgcagtattc actgcaatgc gataaagcca cgtataaaaa
     1141 gcactttcgc cacggaaaga ctctaaagaa cgataggctt taataaaaga ttcttgcaca
     1201 atgtccggaa tatcatctct tgcaacataa cgagtcagca aaccggctac tctattttga
     1261 taacgtacaa caagtaaatt aaatgctttt ttatcacctt tttgtgcttt ttcgactaat
     1321 tcttggtcgg tgatctgttc gctcaactgc ggactctcct aaatatcaat atgtcatttt
     1381 ccattgcaca ctttgttagt gtgtgcttta tgacaaaagt tcaaaaaatt tgaaaaattg
     1441 tgtaattttt tacattccta gataaaacct acttaataaa ctaagcaagc ggtcggattt
     1501 tagcgaaatt ttgcaaaaat ttcaaaaaat ccgaccgctt gtctatgtta tgctttcgct
     1561 ctataagtta tttcttcgct tttaaatctg ccactttttg tgaacggtaa atcgcattaa
     1621 tggcgtgaat taaagctcgg gctgaagatt ccacaatatc ggttgccaaa cccacaccgt
     1681 ggaattttcg accttcatat tccactacaa tatccacttg ccctaatgct tctgctcctt
     1741 cgcctttcgc agtcagatta tagtgcgaca tttttagctc cattcccaca atgttcataa
     1801 tggcattgta agttgcctca actggaccat ttcccccttg agatgtagca ctttgacgaa
     1861 cgccgtctaa ttccacctgt acaaaagccg aagccggtaa accgctgatt agttgggtgg
     1921 taattacatc taattttaag cggtcttcat cgccttgctg catatcaatg aaggcaagag
     1981 cttctaagtc ataatcaaac acttgccctt ttttatcagc aagttgcaag aaggcctcat
     2041 aaagtttatc taaatcgtaa tccgtttccg tataacccat atcagccata tgccctttca
     2101 ccgctgctct acctgaacgg gcagttaaat tcagcttctc ttttttcaag ccgatggttt
     2161 ccggcgacat aatttcataa gtatttttat ttttcagcat accgtcttgg tgaatacctg
     2221 aagagtgagc aaaggcattg ctaccgacaa tcgctttgtt cggctgaatc ggcatattgc
     2281 aaagctggct gaccatttgg ctgacacggt gaatttcttg ggtattaatg cgggtatcaa
     2341 cccccataaa ttcttggcgg gttttaattg ccattaccac ttcttcaaga gcggtgttac
     2401 cggcacgttc gccaataccg ttgatagtac actcaatctg tctcgctccg ttttgcaccg
     2461 ctgtcaatga gttggcggta gccataccta aatcgttatg gcagtgaaca gagacaatcg
     2521 ctttatcgat attcggaaca cggttcatca cttggtggat aatattgccg tattcattcg
     2581 gtaaacagaa accgactgta tccggaatat tcactgtggt tgcaccggca ttaatggcag
     2641 cttctacaat gcggcagatg ttatcaattc cagtacgccc agcatcttca caggaaaact
     2701 caacatcatc agtataattt ctggcgcgtt ttaccgctgc aaccgccatt tcgactacat
     2761 catcaaatga acgttttaat ttagcctcaa cgtgtaatgc cgagctggca ataaaggtat
     2821 ggatacggaa tgcttctgct acttttaagg cttcataagc tgcatcaatg tcttttgcaa
     2881 ccgctcggga aagcgccgcc acccggctgt ttttgatatg acgagcgatg gtttgtactg
     2941 attcaaaatc gcccgctgaa gaaactggaa aaccgacttc catcacatca acccctaaac
     3001 gttctaaagc aagggcaatt tgcaattttt cttttacggt taaacttgct tttaatgatt
     3061 gctcaccatc acgtaaagtc gtatcaaaaa taataatgtt attgttgctc atagaagcct
     3121 ctttttaggt gtttttattt gataaaaaaa gcccgcattt taaggtgcgg gcaagaatgt
     3181 ggatagtcca tttacttttt ccaatcaatc tttatcctcg cacaaaatac gataacagag
     3241 ataatcggag taaggtaaat ttttccatgt tgtgttgtat gtttatgttt gcattgttat
     3301 tcagtagttg aggtgtatat tatccttatc caaatcaatg tcaatagcat ttttctcttc
     3361 acgcaaccgt ttaccagccc attaat
//
LOCUS       DANLXQ010000207         3374 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.211, whole genome shotgun sequence.
ACCESSION   DANLXQ010000207 DANLXQ010000000
VERSION     DANLXQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.211"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            30..1964
                     /gene="cpdB"
                     /locus_tag="PXD94_002481"
     CDS             30..1964
                     /gene="cpdB"
                     /locus_tag="PXD94_002481"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL6287286.1"
                     /translation="MLVLGTQPSAFAQTAKNKAGLRVIATTDIHGFLTDFDYYKDAPT
                     EKFGFTRTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKGAKEGKPEPSIMALN
                     AMQYEVGTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDEPFFTPYVIQQKEIV
                     DEKGNKHTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQKYVPIVKKEGADIVV
                     ALAHTGPSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNKEFANSPNADIAKGT
                     MKGVPESMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYNTEEKKATVENHPEI
                     VALLKETHEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENVVKGLP
                     ELAGLPILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYLYPNTLVVVKATGAE
                     LKEWLECSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSYQFDITQPARYDGEC
                     KLINPNSHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTGDKHIVFAAPDENRQ
                     VLANYITTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPTDKAKAFIKENAQYP
                     MEYIENDETGFAVYRIDLTK"
     gene            complement(2037..>3374)
                     /locus_tag="PXD94_002482"
     CDS             complement(2037..>3374)
                     /locus_tag="PXD94_002482"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL6287287.1"
                     /translation="YAPFVTRVVSFGFQAFILKYLIHYFNDNFFSRSQHDVVSEYTQF
                     ISDTLNIEDSGEHIAQLHQLGYLPIRIKAIPEGKSVAVKVPLMTIENTHSSFFWLTNY
                     LETLINVSLWQPITSASIAFAYRKALAEFSEQTCDDNSHLPFQAHDFSMRGMSSLESA
                     ESSGAGHLTSFLGTDTIPAISFVTAYYGSQHLIGTSIPASEHSVMSSHGVDELPTFRY
                     LMAKYPNNMLSIVADTTDFWHNITVNLPLLKQEILARPESAKLVIRPDSGDFFTIICG
                     NNSSPVEHERKGLIECLWDIFGGSINQKGYKVLNPHIGAIYGDGVTYDKMIRILEGLT
                     AKGFASSNIVFGVGAQTYQRNTRDTLGFAIKATSITINGVEKAIFKAPKTDNGLKKSQ
                     KGRVKVISYDHYVDELTALDDFSDDLLEVVFENGKLVKSTNFDEIRQNIAQQF"
BASE COUNT         1120 a          648 c          689 g          917 t
ORIGIN      
        1 gaaaatttat ccagcttggt gcaactgcaa tgcttgtatt ggggacacaa ccgtctgcat
       61 ttgcacaaac agccaaaaat aaagctggct tgcgtgtcat cgccactacg gatattcacg
      121 gtttcttaac tgattttgat tattacaaag atgctccaac agaaaagttt ggttttaccc
      181 gtacggcaag cctaattaaa caagctagag cagaagttaa aaacagtgtg ttagtcgata
      241 atggtgactt aattcaaggt aatcctattt ctgactacca gtcaaccaaa ggtgcaaaag
      301 agggcaaacc tgaaccttcg attatggcat taaatgcgat gcaatatgaa gtcggaacgc
      361 tgggaaatca tgaatttaac tacggtttaa gctatttaga taatgctatt aagcaagcaa
      421 aattccctat tgtcaatgca aacattgtga agccggggac agatgaacca ttctttacac
      481 cttatgttat tcaacaaaaa gaaattgtag atgaaaaagg gaataagcat accattaaga
      541 ttggttatat cggttttgta ccaccacaag tgatgatttg ggataaagct aatcttgaag
      601 gaaaagttga agctcgagat attgtgaaga ccgcacaaaa atatgtgcct atagtaaaaa
      661 aagaaggggc agatattgtc gttgcattgg cacataccgg cccttcagac gaaccctata
      721 aagaaggcgc tgagaactgt gcgttttatc tagctgatgt aaaaggtatt gatgcagtta
      781 ttttcgggca ttcacatcgc ctgttcccaa ataaagaatt tgcaaattca ccaaatgccg
      841 atattgcaaa aggaacaatg aaaggcgtac cggaaagtat ggctggctac tggggaaata
      901 acatcagtgt gattgattta agcttggccg aaaacgatgg taaatggggc gttgtggacg
      961 gtagagcaga gttgcgtcca atttataata cggaagagaa aaaagcaacc gttgagaacc
     1021 accctgaaat cgttgcatta ttaaaagaaa cccatgaagc aacccgtcaa tttgtgtctc
     1081 aacctatcgg taaagcaact gataatatgt atagttacct tgcgttagtt caagatgacc
     1141 ctaccattca aattgtgaac caagctcaaa aagcgtatgt agaaaatgtg gtaaaaggct
     1201 tgccggagct tgcaggatta ccaattctaa gtgccggagc accgtttaaa gccggtggac
     1261 gtaaaaatga cccgacaggc tataccgaag tcgataaagg cgaattaact ttccgcaatg
     1321 cggcagactt atacctttac ccaaatacct tagtggtggt taaagcaaca ggagcagaat
     1381 taaaagagtg gttagagtgc agtgcaggaa tgttcaaaca aattgatgtc gcttcggata
     1441 aaccgcaata tttactggat tggacaggtt tccgcaccta taactacgat gtaattgacg
     1501 gtgtcagcta tcaatttgat ataacccaac cggctcgtta tgacggcgag tgcaaattga
     1561 tcaaccctaa ttcgcaccgt gtggtgaact taacctttga cggcaaaccg gttgatccta
     1621 aacaagaatt cttaattgcc accaacaatt accgtgctta tggagcaaaa ttcccgggaa
     1681 caggcgataa acatattgtg ttcgccgcac cggacgaaaa ccgccaagtg ttagcgaatt
     1741 atattaccac cgaaagtaaa gctcacgggc aggtaagtcc gaaagcggat aacaactggc
     1801 gtattgcacc aattagctca aatgcaaaat tggatattcg ttttgaaacc tctccaacag
     1861 ataaagctaa agctttcatt aaggaaaatg cacaatatcc gatggagtat atcgaaaacg
     1921 atgaaaccgg cttcgcagtt taccgtattg atttaaccaa ataatcagag ctaaatacaa
     1981 gcggtctaat ttgcaaaaaa attaacaaat ttgaccgctt gtttcattcc atttttctaa
     2041 aattgttgtg ctatattttg ccgaatttca tcaaaattgg tcgatttgac caacttacca
     2101 ttttcaaaaa ccacctctaa aagatcatct gaaaaatcat ccagtgcggt tagctcgtca
     2161 acataatgat cataagagat tacttttact cgcccttttt gcgatttttt taagccgtta
     2221 tcggttttag gagctttgaa aattgccttt tcgacaccgt taattgtaat tgaagtcgct
     2281 ttgattgcaa aacccagcgt atcacgagta ttgcgttgat aagtttgagc cccaacgcca
     2341 aacacaatat tgctggaagc aaaacctttt gccgttaagc cctctaaaat ccgtatcatt
     2401 ttatcataag tgacgccgtc accataaatt gccccaatat gcggattaag cactttataa
     2461 cctttttggt taatactacc accaaaaata tcccacaaac actcaatcag acctttgcgt
     2521 tcgtgttcaa cgggggagga attgttaccg caaataattg taaaaaaatc accgctatca
     2581 gggcggataa ccaattttgc tgattccggt cgagctaaaa tttcttgttt taataatggc
     2641 aaattaacgg tgatgttatg ccagaaatcg gttgtatctg ccacaataga tagcatatta
     2701 tttggatatt ttgccattaa ataacggaaa gtcggcagct catcaacacc gtgtgaactc
     2761 atcaccgaat gttctgatgc cggaatagat gtaccgatta aatgttgaga accataatag
     2821 gctgtcacga atgaaattgc aggaatggta tctgtgccta aaaatgaggt tagatgtccg
     2881 gcaccggagc tttctgctga ttctaaggaa ctcattccac gcatagagaa atcgtgtgcc
     2941 tggaagggta gatggctatt atcatcacag gtttgctctg aaaattcggc tagagcctta
     3001 cgataagcaa atgcaatgga agctgatgta ataggctgcc ataatgacac attaatcagg
     3061 gtttccaaat aattagtcag ccaaaaaaag cttgagtgag tgttttcaat tgtcattaaa
     3121 ggaactttca ctgcaacaga ttttccttct ggaatagctt taattctgat aggtaagtaa
     3181 ccgagctgat gcaattgtgc aatatgttcg ccagagtcct caatattcaa tgtgtcagaa
     3241 ataaattgag tatattcaga aacaacgtca tgttgtgagc gagaaaaaaa attatcgtta
     3301 aaataatgaa tcaagtattt taaaataaat gcctgaaagc caaatgatac aacacgtgta
     3361 acaaatggag cata
//
LOCUS       DANLXQ010000208         3336 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.212, whole genome shotgun sequence.
ACCESSION   DANLXQ010000208 DANLXQ010000000
VERSION     DANLXQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.212"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            241..429
                     /locus_tag="PXD94_002483"
     CDS             241..429
                     /locus_tag="PXD94_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287288.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
     gene            470..772
                     /locus_tag="PXD94_002484"
     CDS             470..772
                     /locus_tag="PXD94_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287289.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            772..2082
                     /locus_tag="PXD94_002485"
     CDS             772..2082
                     /locus_tag="PXD94_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL6287290.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRC
                     VITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDAT
                     VIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFS
                     GWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMG
                     NWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSFEALRAKILFTEGFQKIKKPR
                     YQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVREIEAGRL"
     gene            complement(2178..>3336)
                     /gene="tet(H)"
                     /locus_tag="PXD94_002486"
     CDS             complement(2178..>3336)
                     /gene="tet(H)"
                     /locus_tag="PXD94_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL6287291.1"
                     /translation="AIGIGLIMPVLPTLLNEFVSENSLATHYGVLLALYATMQVIFAP
                     ILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRIIAGITGATGAVCASA
                     MSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMPFIFAAISHSILLILS
                     LLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATYFIIQLIGQIPATIWV
                     LFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEKTTIMISMSIDMMGCL
                     LLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGKLQGTLVSLTNITGII
                     GPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTTPKAVISTP"
BASE COUNT          960 a          801 c          799 g          776 t
ORIGIN      
        1 ggttctcttt ttttacatta atttcgacac aacatcttta atttgatagt atttatatat
       61 gatattttgg gtatccggat ttagcggttg atctgcgcta gacgtaattt tgtaaataaa
      121 ccggatttta gggttgacgg aaaaggccag atgccctaat attagattca agagctgaga
      181 acaacggctc gggcataagc ccaacgggcg caccgccgaa cttggtgcag gatagaagcc
      241 atgtcatacg atgcaaatga tgcgctgaat gaaatcgaag aggcgcttag cgagcttgaa
      301 agggttgctg aagacctaat caacaacaac ccgaataaag aatcagaatt acgcggccaa
      361 ggggttcacc aagcgacaaa gcacctccgt tttcgtattc gcaacatccg ccgcggcgaa
      421 gcaatttgac taacatgccg ccctaggggc ggctttcagc gaggtcagca tgaaaatcaa
      481 actctatttt ccggacgagt ctatcgccac gataaagcga atggggcttc gccaaatgtc
      541 tcccgagact ctcaggtgga ctgccgatca tccgtcaagc agctatggta tgggcgcttt
      601 gctcagaggc aagtctggtg aaatccttga tgggaagtct tttgctgcaa tggtgcatgc
      661 ctttggcgcg tggattgaaa cagacagtga agataccagt cgtcgagttc ataacgcgct
      721 agtcactgct gccactggca ccgaggaatc ggttaaggtg gcaaaggagt aatgtcgaat
      781 atcctcaact ggcctgatta caaagtgctc caggtatccg aactggagca cgattaccag
      841 gtacacgccg aagtttccga accgcctacg cagtgccccc actgcaatca tcctgaaatc
      901 gtcggctttg gtcgccgtga tgaggtgatc atggatacac ccgtccacgg aaggcgtacc
      961 ggcatcatgc ttaaccgccg tcgctatcgc tgccagtctt gccgaaagac gtttctggag
     1021 cctgtaccgc acaaagacga aaagcggcag atgaccaacc gcctgatcca gtacatcgag
     1081 cgcgagagcc tgcgccgcac gttctccagt gttgccgagg atgtgggcgt cgatgaaaag
     1141 accgtccgta acattttcaa cgactattgt gaacgcctcg aaaagacgct caattttgaa
     1201 atgccgcagt ggttgggtat cgacgaaatc cacatcatca aaccccgctg cgtgatcacc
     1261 aacattcagc agcagaccat tgtggacatg ctggataacc gcaataaaac caccgtcacc
     1321 cgctacctga gcaaacgcac tgaccgcgac ctggtgcgct acgtggcaat ggatatgtgg
     1381 aggccgtacc ggcaagcagt agaaaccatg attcctgacg ccacggtgat catcgacaag
     1441 ttccacgttg tcaggatggc gaacgaatca ctggaacgcg ccagaaaagc catcagaagc
     1501 gccctgacgc cacagcagcg ccgtgggttg atgcgagata ggtttgtcct actgaaacgc
     1561 cgtcacgagc tgacagatgc cgaatacatg cgtttttcag gctggacatt gaattaccct
     1621 gagattggcc aagcctacga actgaaagaa gcctttttcg agatttggga ctgtcagacc
     1681 cggcaccagg cacaggaggc atattactct tggctgcgac agattacgcc ggaaatgaaa
     1741 gcccactatg acccgcttat caaagcgatg ggcaactggc acgatgacat tttcgcttac
     1801 ttcgaccacc ccattaccaa cgcctacacc gagtcactga acaacctgat tcgtgttgtt
     1861 aatcgtgttg gccgtggcta cagctttgaa gcgctgcgag ccaagatcct gtttactgag
     1921 ggcttccaga agatcaagaa gccacgctat cagcgtcaac ggatacccga gggcgctatg
     1981 ggcaggatgc cgttctatgg cgttgccgaa gcggggccat ccactaacta cggcgctgac
     2041 atatcaacac tggtgcggga gattgaggcg gggcgtttat agccctgttt caaccctgta
     2101 atccggatac ccaaataaag tcctatcaac gttattaaga gcggacagga cttttatgat
     2161 ctttataatt tctagtatta aggggttgaa ataacagctt taggtgtggt ttttctttgg
     2221 tgaaaataag cggtaataag caacatagca taaagtattg cccccatcag ccataacaga
     2281 ccatcccaat aagcgacgct ataactataa ataaaggcaa ataaaagggg accaatgatc
     2341 ccggtaatat tggttaggct caccagagta ccttgtaatt tcccttgcgc attatcatcg
     2401 acagattttg ataaataacc ttgtaatgcg ggttgcccca tacctcctgc cgctaagcaa
     2461 attaatgctg gtaagatgac ccaaacgtgg cctatccacg ctaataataa acagcccatc
     2521 atatcaatag acatactgat cataatggtg gttttttcgc cccatttttg tgccaatttc
     2581 ccagcgacaa tcgcctgaaa gaaaatattt aatacaccca gaaccgccaa agacataccg
     2641 atagaagttg tgttccaatc aaaacgatat tgtgtaaaca gcacccagat ggtggcagga
     2701 atttgcccga taagctggat aataaaatag gttgctaacc aaaagtagag gcttttctta
     2761 aaaaaaacag tgactgtatt tgaggcagtt tggttttcag gtgtcctatt ggcaacaagc
     2821 gcttctcttt tttgtgtttc tcggaaaaag agcaaagaga gtattaataa tatcgagtgt
     2881 gaaatagcgg caaaaataaa tggcatatga gcactgatat cacctaataa tccccctagc
     2941 attgggccga taataaggcc aacaccaaaa gcaccaccta agaaaccaaa atagcgagtt
     3001 cgatttttag cgggagtcac atcactcatc gctgatgcac atacggcacc tgttgcgcct
     3061 gtgatccccg caatgatgcg cccaatatag agcatccaaa gtgtggttga gaatgccatt
     3121 aaaagatagt cgagtgccgc gcctaaaagg gaaaacagca agatgggttt tctgccgtat
     3181 ttatcagaca gtcgtcctag aataggagca aaaataacct gcatggtagc atagagcgct
     3241 aatagcacac cgtaatgggt tgccagtgaa ttttcactga caaattcatt taatagagta
     3301 gggagtactg gcatgataag cccgatacca atggca
//
LOCUS       DANLXQ010000209         3333 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.213, whole genome shotgun sequence.
ACCESSION   DANLXQ010000209 DANLXQ010000000
VERSION     DANLXQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.213"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            1..2091
                     /locus_tag="PXD94_002487"
     CDS             1..2091
                     /locus_tag="PXD94_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL6287292.1"
                     /translation="MLSYLVFFSKIIKIPNLKGFLPHCEIRRRPLVGKTFIAQKRPNQ
                     IIGWGLRPSAVGSRAYAQKHQLPYIALEDGFLRSLGLGVKGYPPFSLVYDDIGIYYDT
                     TCPSRLEKLLLEKPLQDQEQIKLAERALSQILTYGLSKYNHALDFSEIPSAKEKIVLV
                     IDQTFGDMAVKYGQASEVDFNKMLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQ
                     YTDRVRIITEDVNPASLIKLCEKVYCVTSQMGFEALLLGKKVVTFGVPWFAGWGVTDD
                     RNEAVQLLVQQNRRKDRSLLQLFYAAYFDYSRYINPNTGEKGSIFDVIDYLYKAKRLN
                     QRLSGNLYCIGMSLWKQAVIKPFFNLPQCRLHFVRSLNSLKKRPLADNAKILVWSNGK
                     ADVLQFAQQNNIPLLRMEDGFIRSVGLGANLVAPLSLVVDDLGIYFNAENTSRLEQIL
                     LSAEHFSETDLSYAETLRQKLVASHIGKYNVGGGDVKFNAQGKKIILVPGQVEDDASI
                     KSGSPQIKRNLDLLKKVRELNPSAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDN
                     ANILDCILIADEVHTMTSLAGFEALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSL
                     TLSQLIAATLIYYPLYVDPISRQFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKL
                     QHLYRAFKYASHTK"
     gene            2114..>3333
                     /locus_tag="PXD94_002488"
     CDS             2114..>3333
                     /locus_tag="PXD94_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL6287293.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYAELPVVSEPEKVSK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYQHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGFIDYKMVIYEFLEQYPQLNGRVFYIHDVPLPVFLRYGKGMVTLNSTSGLSA
                     LLHNMPVKTLGRANYDFEGLTDKQPLDTFWANPQPPNSEVFKGYRQFHLHKTHINGNF
                     YHKSILRYPYNWEN"
BASE COUNT         1061 a          538 c          687 g         1047 t
ORIGIN      
        1 atgcttagct atttagtgtt ttttagtaaa attataaaaa tccctaattt aaaaggtttt
       61 ttacctcatt gtgaaattag gcgaagacct cttgtgggaa aaacctttat tgctcaaaaa
      121 agaccgaacc aaatcatcgg ctgggggctt cgtccttcag cggtgggatc aagagcttat
      181 gcacaaaaac atcaacttcc ctatattgca ttagaagacg gctttttacg ctcactaggg
      241 ctgggtgtaa aaggctatcc gccattttct ttagtttatg atgatatagg tatttattac
      301 gataccacct gtccttctcg tttagaaaag ttattgcttg aaaaaccttt acaggatcaa
      361 gagcaaatca agcttgcaga gagagcatta tctcaaatct taacttacgg actttctaaa
      421 tataatcacg cactagattt tagtgaaatc ccctctgcca aagaaaaaat cgtgttagtt
      481 attgaccaaa cctttggtga tatggcggtg aaatatggtc aagcgagtga agtggatttc
      541 aataaaatgt tggaaacggc aatcagtgag aatccagagg ctcaaatttg ggtaaaaact
      601 caccccgatg tattaagtgg caagaagaaa ggctatttga ccgatttacc tcaatatact
      661 gatagggtca gaattattac tgaagatgtg aatccggcaa gtttgattaa attgtgtgag
      721 aaagtctatt gcgtaacttc gcaaatgggg tttgaggcat tattattagg taaaaaagtg
      781 gttacctttg gtgtaccgtg gtttgccggt tggggggtaa cagatgatcg taacgaagcg
      841 gttcaactat tagttcaaca aaatagacgg aaagatcgtt cattactgca acttttttat
      901 gctgcgtatt ttgattacag ccgttatatt aatccgaata ccggtgagaa aggctctatt
      961 tttgatgtca tcgattatct atataaagcc aaaagattaa atcagcgctt aagtgggaat
     1021 ctatactgta ttggtatgtc gctatggaaa caggcggtaa ttaagccgtt ttttaatttg
     1081 ccacaatgtc gattacattt tgttcgttcg ctaaattctt taaaaaaacg accgcttgca
     1141 gataatgcaa aaattttagt ttggagcaat ggcaaagcgg atgtcttgca atttgcacag
     1201 cagaataata ttccattgtt aagaatggaa gatggattca ttcgctctgt cggactaggc
     1261 gctaatttag ttgcaccgct ttctttagta gtcgatgatt taggcattta ttttaatgca
     1321 gagaacactt cacgattaga acaaatttta ttgagtgcag agcatttttc tgaaactgat
     1381 ttgtcttatg ctgaaacatt aaggcagaaa ttggtggcta gccatattgg aaaatataat
     1441 gtgggcggtg gcgatgtaaa atttaatgct caaggtaaaa aaataattct tgtaccgggg
     1501 caggtagaag atgacgcttc gatcaaatca ggttctccac aaattaaaag aaatctggat
     1561 ttactgaaaa aagtgaggga attaaaccct agtgcctata ttatttataa gcctcaccct
     1621 gatgtggtaa gtggtaatcg agtcggtcag attcctacgg cagaaacacg caggtttgcg
     1681 gatcagatgg tggataatgc caatatttta gattgtattt taattgcaga tgaagtgcat
     1741 acaatgacat ctttagccgg tttcgaagca ttattaagag gtaaaaaagt gcattgctac
     1801 ggattacctt tttatgctgg ttggggattg acggaagata gattgaagtc ggcacgcaga
     1861 ggtaaatctt tgacattatc tcagttaatt gccgcaacat tgatatatta ccctctgtat
     1921 gttgatccta taagcaggca gtttattgat gccaatcaag cgattcatat attgtcacaa
     1981 cagaaacgaa acctaaccaa tcaaaggatt aaacgttctt gggtgagtaa acaatatgga
     2041 aaattacagc atctttatcg tgcgtttaaa tatgcttccc acactaaata agcgtagagg
     2101 gtaggaaatt aagatggaac attatttaga tgagctaatc atcaatagcc agagaatttt
     2161 attgttacaa ggcccgatag gctctttttt cactgagttt gcccgttggt tagaaagtga
     2221 gcaaaaagag gtcttcaagt taaattttaa tggcggagat gaatatttct atccaaataa
     2281 tagcccgaat acttttcctt actatgatag cttagataat tttgaagcct atttaattca
     2341 gttttgccaa actcatcaaa tagataatat cttatgtttt ggcgataatc gttattatca
     2401 taaagtggct aaaaacgttt gcttgaagtt aaagttggtc ttttgggcgt ttgaagaagg
     2461 gtactttagg ccagaatata ttacgttgga aaaatggggt gtaaatgctt tttctccaat
     2521 ccctaaaaat cacactttct ttttgcctta tgccgagtta ccagtagtca gtgaaccaga
     2581 aaaagtatct aaaggcttta gatgtatggc gggaaaagca atcccgtact attggaatgc
     2641 ctataaaaaa cgtaaagcat acgcaaaata tcagcatcat cgttttctga atctcaaata
     2701 ctaccttaat ttatggacaa ggtcgggaat taagcgaata tgttattggt tacatgataa
     2761 aggctttgcc aagcgggtag aaaaaggaga gtttggtgaa tttttcattg tgcctctaca
     2821 agtttatgat gacactcaag tgaggattca tagtgaacaa aaaagtgtag agcgtttttt
     2881 aagggaagtt ctgaattcct ttgccaaatt tgcaccggca catctcaatt taattattaa
     2941 gcatcacccg atggatagag gatttataga ttataaaatg gtgatttatg aattcttaga
     3001 gcaatatccc caattaaacg gtagagtttt ttatattcac gatgtgccgt tacctgtctt
     3061 tttacgttat gggaaaggaa tggtgacttt aaattcaacc agtgggcttt ctgcattgct
     3121 ccataatatg ccggtcaaaa ccttaggtag agccaattat gattttgagg gattaaccga
     3181 taaacagcca ttagatactt tttgggctaa tcctcagcca cctaattcgg aagtatttaa
     3241 aggctacaga caatttcatt tacataaaac gcatattaac ggtaattttt accataaatc
     3301 gattttgcgt tatccttata attgggaaaa tga
//
LOCUS       DANLXQ010000210         3235 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.214, whole genome shotgun sequence.
ACCESSION   DANLXQ010000210 DANLXQ010000000
VERSION     DANLXQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.214"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..567)
                     /gene="pdxT"
                     /locus_tag="PXD94_002489"
     CDS             complement(<1..567)
                     /gene="pdxT"
                     /locus_tag="PXD94_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL6287294.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCRKN"
     gene            complement(567..1439)
                     /gene="pdxS"
                     /locus_tag="PXD94_002490"
     CDS             complement(567..1439)
                     /gene="pdxS"
                     /locus_tag="PXD94_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL6287295.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGVATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSEDLGEAMVGI
                     NEQEIELLMAARGV"
     gene            1650..3053
                     /locus_tag="PXD94_002491"
     CDS             1650..3053
                     /locus_tag="PXD94_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL6287296.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIVEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQNAFIIGYSNLKD
                     DQINYVAESLKKILDNE"
BASE COUNT         1005 a          646 c          596 g          988 t
ORIGIN      
        1 attttttcgg cacatttcca caaaatagcg gtgaaaactt aaatcattat tcacttcggg
       61 atggaaggat atacctatct gattttggta ttttactcca acaatcttac cgcttatctt
      121 ggctaaaatt tctacatctt ttccagtgct ttctatataa ggcgcacgaa tgaatgtgag
      181 aggaatttta ccgagatatt taacttcttc ttctactaaa aacgagccta attgccttcc
      241 gtaagcatta cgtttcactg ttacaggcaa agtagccaaa tgttttttat cgtcgtttac
      301 caaattttcc gcaagcaaaa ttaaacccgc acaagttcca agcgttggca agccatttaa
      361 aattttttga cgaagcggct caagcaagcc taattcacgt aataatttac cttgaacagt
      421 cgattcgcca ccaggtaaaa tcagaccatc aaaatcttgg tttaaatcag caagttgacg
      481 aatttccata ctttcaaccc ctagttgctg gagtttatca gtatgctcgc taaatgcacc
      541 ttgcagagca agaatagcaa ttttcattag acacctcttg ctgccattag caattcaatc
      601 tcttgctcat taattcctac cattgcttca cctaaatctt cagaaagctc cgcaagtaat
      661 ttaggatcgt tgtagttggt aacagcttgt acaatggccc gcgctcgttt agcaggatcg
      721 cctgacttga aaatacctga gccaacgaag actccttctg cacctaactg catcatcaat
      781 gcagcatcgg caggggttgc aacacctcca gcagcaaagg tgacaacagg caatttttgg
      841 tactgatgta catattgtac taaatcaaat ggtacttgga gctctttagc tacgtgataa
      901 agttcatctg tggtatgtga aaccaagcgg gcaatttctt gtttcatttt gcgaagatga
      961 tgtactgctt gcaccacatt gcctgttcct ggctcgccct tactacggat catagcagcg
     1021 ccttcattaa tacgacgtag ggcttcgcct aaattttttg ctccacatac aaatggcact
     1081 tcaaatttac gcttgttaat gtggaaggta tcatctgcag gagaaagcac ctcactttcg
     1141 tcaatatagt caatttcgat cgcttgcagg atttgtgctt ctgtaacgtg tccaatacgt
     1201 actttcgcca ttacaggaat ggataccgct gcttggatac tctttatcat cttgggatca
     1261 ctcatacgag aaacgccacc agctgcacga atatctgcag gaattctctc tagtgccatt
     1321 actgcacaag ctccagcttc ttccgcaatg caggcttgct caggggtagt tacatccata
     1381 attacaccgc cttttaacat ttgagcaagt tgtctgttta aatcatagcg attagtcatt
     1441 gtactttttc cttctaattg ttgaataatt gaacttttcg tagtttcgat tagctcggca
     1501 ttaagtttat ttttatattc gctggatgcg agtgttcatc taccaattta aatacactca
     1561 gccaatttga gtgaggaaac agcatgtatt gtaatttaat ctgaactgtt taaaatgacc
     1621 attttatgtt tttcttatca tgtcagatta tgcttaccta cacttttaac acacaagaag
     1681 gcaccgctct ctatgagcaa ctttaccgtt ttctaaaaaa cgacattgaa agcaacatac
     1741 ttatagcagg ggataaactg ccgtctaaac gggcttttgc aaagcattta ggcatttcag
     1801 taatgacagt ggaaacagct taccaacagt tagtagcaga aggttatgtg aatactcagc
     1861 ctaaaaaagg cttttttgtg aatgctttaa atttaccaga aaaaaaggaa gccatatcag
     1921 ttcagctaca aaaaatcagt tcagaacagt atgagaaaaa taaaaaatgg caggcagatt
     1981 taactaatcg acagacttta gcagaaaatt ttccgttttc ggtttggaca aaattagtac
     2041 gcgaagtgct gaaaacacac caaagtgagc taatggagcg tgctgagagt gggggcgtat
     2101 ggacattaaa atttgcgata gccaatcatc ttcgagcctt tagaggaatg atcgttaatc
     2161 caacacagat tattattgga gcgggtacgg agtatcttta cggtttgctc attcagcttc
     2221 taggcttaga taaacattat gcactaactg agcccagtta cgataaattg cgcagaattt
     2281 atcacagtta tcgcttatca acagccacag tatcgatgcc attttcaatc gatgagttaa
     2341 attcaaagaa tgtggacgtg atacatacct cgccatcaca ccattttccg acaggacacg
     2401 taatgccgat tgccaaacgt tatgagttgt tagcttgggt tgcacaaaaa gctgaaagat
     2461 atattgtaga agacgactac gacagcgaat ttcgttttgt aggacaacca attcctgcat
     2521 tgaaatctat tgatatgctc gggcgagtta tctatatgaa tactttttct aaaacgctct
     2581 cttcaactgt acgaattgcc tatatggttt tgccaccaga attactgaat cgttttcagc
     2641 aggaattagg gttttatgct tctaccgttt ccaattttga gcaatatatt ttggcagaat
     2701 ttattcaaca aggctatttt gaaaagcata tcaatcgaat gcgagcgtat tatcaaaaaa
     2761 agagggataa attactaaaa gctttcaagc aaggcaattt agcagaaaag attgcaataa
     2821 aagaagaaca ttcaggcttg catttcattg tacagctaaa cacaacatta acagaaaggc
     2881 aaattttgca ggcagcagaa gaacaaggta taaaatttgt cgcattgtct gcttattatc
     2941 aagataaatc tcaaattcag caaaatgctt ttataattgg gtactctaat ttaaaagatg
     3001 atcagattaa ttatgtagca gagagcctga aaaaaatttt agacaacgaa tagcaagtat
     3061 caaaactgct ttaattttta gcaaattgcc ttaaaaacac gcaagtggta attattttag
     3121 tgatttttgc aaaaaattac caaattctcc ttgcaaggca ttttgttttg cgtataatgc
     3181 atggcacaca acgacgcact gttgtgaacg atttaaaatt gatttccagt gcgtc
//
LOCUS       DANLXQ010000211         3212 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.215, whole genome shotgun sequence.
ACCESSION   DANLXQ010000211 DANLXQ010000000
VERSION     DANLXQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.215"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            6..842
                     /locus_tag="PXD94_002492"
     CDS             6..842
                     /locus_tag="PXD94_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL6287297.1"
                     /translation="MLFACINTLIPKLTSISPIDSNVIALVQYLAAFIFLVPSMNNIG
                     FFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGIALLMTSPLFVTIGSGLF
                     LKEKVDKKRWIATAMGFVGAMIILEPWSDHFEMIALLPIAAAFFWAGYSLMVKKLSRY
                     DSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGFLILLGFFTALAQLAVAK
                     AYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGAAIIIAATIYITHAETKQ
                     SN"
     gene            complement(921..1412)
                     /gene="ilvN"
                     /locus_tag="PXD94_002493"
     CDS             complement(921..1412)
                     /gene="ilvN"
                     /locus_tag="PXD94_002493"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL6287298.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(1405..3126)
                     /locus_tag="PXD94_002494"
     CDS             complement(1405..3126)
                     /locus_tag="PXD94_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL6287299.1"
                     /translation="MKKLSGAEMIVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLGLNH
                     VLVRHEQGAVHMADGYARSTGKVGCVLVTSGPGATNTITGILTAYTDSVPLVILSGQV
                     PSSLIGTDAFQESDMIGISRPVVKHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDI
                     PKDVVNPLNKYPYEYPQEVSMRSYNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVIS
                     AEGSPEITEFAQKLNLPVTCSLMGLGAYPASDKQYLGMLGMHGTFEANNAMHESDLIL
                     GIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVQAYIPIVGSAKDVMEEFLSLL
                     GEENLAKNQVDLTAWWQQIDAWKARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVAS
                     DVGQHQMFAALHYGFDKPRRWINSGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSI
                     QMNIQELSTAKQYDTPIVIVSLNNRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAE
                     AYGHVGIQIDHPSELEAKLTEAFSIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLS
                     KTERTDA"
BASE COUNT          884 a          695 c          603 g         1030 t
ORIGIN      
        1 ccggtgtgtt atttgcctgt atcaatacct taattcccaa attaacgtcc atttcaccga
       61 ttgactcgaa cgtgattgca ttggtgcaat atttagctgc ctttattttc ctcgttccca
      121 gcatgaataa tatcggtttc ttctcttcat taaaaaccga gcattttggg caacactgtt
      181 tccgtatttt tctatctgcg attggtattc aatgttggac aatggcactc gctaaccaat
      241 tacctatttg gcagggaatt gcgttattga tgacttctcc attgtttgtg accatcggct
      301 ccggtctgtt tttgaaagag aaagtggata aaaagcgttg gattgcaacc gcaatggggt
      361 ttgttggggc gatgattatt ctagagcctt ggagcgatca ttttgaaatg attgcgttac
      421 tgccgattgc cgcggctttt ttctgggcag gttattcgct aatggtgaaa aaactctctc
      481 gttacgatag cccaactacg atggtcgctt acctgtttat tctaattacg ccttttaatt
      541 tgttgattgc actcacgaac cttagcccta gtgggtttag tctgccttct tggagcgatt
      601 tcggcttttt aatcttactt ggtttcttta cggcgttagc gcaactggcg gtagccaaag
      661 cctacagtgt agcagatgct tcctacattc agccgtttga ttttattaaa ttacctttga
      721 acgtattagc cggttggctt gtatttaact gggtgccacc gggtaaatta tggctaggag
      781 ctgcgattat tattgctgcg accatttata ttactcacgc tgaaactaaa cagagtaatt
      841 agcaacaaat tcacgctaca taaaaatccc tttatctcaa agataaaggg atttgcaaat
      901 tttaagctaa aattgaccgc ttacagagcg tttttatcgc cacgagatag actaatcacg
      961 ccagagcgaa caatctctac aatcgttgtt tcttctttaa ctgcctcaat aaatgcattg
     1021 agcttctcgg tagaaccgga taattgaatg gtgtaaagtt tcggtgttaa atctacgatt
     1081 tgcccacggt aaatatctac catacgtttc agttcatcac gagatgaacc tgtagctcgc
     1141 acttttacca ataacacttc acgctcaacg tgttcataag cacttaagtt actcacttta
     1201 aacacatcaa tgagtttgtg tagttgcttt tcgatttgtt ctaacacctg ttcttcgcct
     1261 tgcgcgacaa ttgtcatacg ggaaaggctt ggatcatcag taggggcaac ggtcaagctt
     1321 tcaatgttaa aaccacgctg cgagaaaagg gcaactacac gagataatgc acctgattca
     1381 ttttctaata atacggctaa tgttctacgc atctgttctc tccgttttac ttaacatcat
     1441 ttcattcatt gctcctccac gcacttgcat tgggtaaaca tgttccgttt catcgaccaa
     1501 tacatcaaca aatacaagct tatctttaat tgagaatgcc tcagttaatt ttgcctccaa
     1561 ttcagaaggg tggtcgattt gaatcccaac gtggccataa gcctctgcta atttagcaaa
     1621 gttaggtaga gaattcatat acacctgtga atggcggcca gagtaaataa tatcttgcca
     1681 ctgtttcacc atacctaaga aacggttatt taagctgaca atcacaatcg gtgtgtcgta
     1741 ttgctttgcg gtggaaagtt cctggatatt catttggata ctaccatcac cggttacaca
     1801 aacaacagtg gcatcagggt gggcaaattt tacccccatc gcagcaggta agccaaagcc
     1861 cattgtgcct gcaccgcctg aattaatcca acgacgtggt ttatcgaacc cataatgcaa
     1921 tgcagcaaac atttgatgtt gtccaacatc tgaggcaaca taagcctccc cattggtcaa
     1981 cttgtggatc attctaatca cttgttgtgg cttaatcaca tcactgtttt cttcaaaggc
     2041 taagcaatta cgagctttcc aagcatcaat ttgttgccac caagcggtta aatctacctg
     2101 attttttgca agattttcct cacctaataa agataaaaat tcttccatta catccttagc
     2161 actgcctaca attggaatat aagcttgtac tgttttggaa attgaggctg gatcaatatc
     2221 cacgtggatc acttttgcat tcgggcaata ttttgctaag ttatttgttg tacgatcatc
     2281 aaaacgtacc ccaataccta aaatcaaatc actttcgtgc attgcattat ttgcctcaaa
     2341 agtgccgtgc atgcctaaca tacctaaata ttgcttatca gaggcaggat aagcacctaa
     2401 tcccattaat gaacaggtta caggtagatt tagcttttgt gcaaactctg taatttctgg
     2461 cgaaccctct gcactaatta caccaccgcc tacataaagc actggacgtt ttgctactaa
     2521 tagtgctttt aatgcttttt taatttgccc tttatgccct tgaactgttg ggttataaga
     2581 acgcattgat acttcttgcg gatattcata gggatattta tttaatggat tgactacatc
     2641 tttaggaata tcaataacta ccgggcctgg tcgaccggtt gatgcaatat aaaaggcttt
     2701 tttgataatt tcaggaattt cttctgtatt ttttactaag aaactgtgtt ttacaaccgg
     2761 acgagaaatg ccgatcatat cactttcttg gaatgcatct gtcccaatta aacttgatgg
     2821 aacttggccc gacagtatta ctaacggaac agagtcggta taagcagtta aaataccagt
     2881 aattgtattc gttgctcccg gacctgaagt cactagcaca cagccgactt tacctgttga
     2941 acgggcatag ccatcagcca tatgtacagc accttgctcg tgtcgaacta aaacgtgatt
     3001 caatccaagt gtatgaattg catcataaat atcgagtaca gagccacccg gatagccgaa
     3061 aacgtattct acgccttcat cctttaagga ctgtacaatc atttctgcac cagaaagttt
     3121 tttcattgaa tcctccaaca aaatagcatt ttcttctcta agatggttgg attttgcagt
     3181 aaaattttat tgatgtctag ttcttattat gg
//
LOCUS       DANLXQ010000212         3144 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.216, whole genome shotgun sequence.
ACCESSION   DANLXQ010000212 DANLXQ010000000
VERSION     DANLXQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.216"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            29..1123
                     /locus_tag="PXD94_002495"
     CDS             29..1123
                     /locus_tag="PXD94_002495"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL6287300.1"
                     /translation="MQFINIANEGVKSLSPYQAGKPIEELERELGIQNIIKLASNENP
                     FGFPESAKKAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFA
                     HTFAGEQDEIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIF
                     IANPNNPTGNFLTQAEIEGFLAKVPANVIVVLDEAYTEFTAPQERVDSFGLLAKYPNL
                     IVSRSLSKAYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASAIAVMNDDEFVK
                     KVAENNRLEMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEALLREGVIVRSIA
                     GYGMPNHLRISIGLPQENERFFTALLKVLA"
     gene            1169..2467
                     /gene="aroA"
                     /locus_tag="PXD94_002496"
     CDS             1169..2467
                     /gene="aroA"
                     /locus_tag="PXD94_002496"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL6287301.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGEEKSQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAEGDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(2521..3132)
                     /locus_tag="PXD94_002497"
     CDS             complement(2521..3132)
                     /locus_tag="PXD94_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL6287302.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
BASE COUNT          927 a          621 c          692 g          904 t
ORIGIN      
        1 tattttataa gaataatcag aggttattat gcaatttatc aacatcgcaa atgagggcgt
       61 gaaatccctt tcgccttacc aagcaggaaa gccgattgag gagctagagc gtgaattagg
      121 gattcaaaat atcattaaat tagcctcgaa cgaaaaccca tttggttttc ctgaaagtgc
      181 aaaaaaggcg attctgaatc agcttgacga tttaacccgc tatccggatt caaacggctt
      241 tgaattaaaa accactattg cccaaaaatt tggcgtacaa gccaatcaaa tcaccttagg
      301 caatggctca aacgatttat tagagctgtt tgcccacacc ttcgccggtg agcaagatga
      361 aattatctat tctcagtatg cgtttattgt gtatccgttg gtgacgaaag cgattaatgc
      421 cgttgcccgt gaaattctgg ctaaaaattg gggacacaat ttagatgcct ttttggctgc
      481 aattaccgaa aaaactaaat taatttttat tgccaacccg aataatccga ccggtaattt
      541 tttaacccaa gcggaaattg agggcttttt agctaaagtg ccggcaaatg taatcgtggt
      601 gctggacgaa gcttataccg agtttactgc gcctcaagaa cgggtggatt cgtttggctt
      661 attggcaaaa tacccgaatt taattgtgtc acgttcactc tctaaggctt acggcttagc
      721 cgggcttcgg attggttatg cggtttcaaa ccctgaaatt gcggatttat taaatcgtgt
      781 tcgccagccg tttaactgca atagtcttgc cttagcttca gcgattgcgg taatgaatga
      841 tgatgaattt gtcaaaaaag tggcagaaaa taaccgcttg gaaatggctc gctatgaggc
      901 tttctgccaa gcaaacggct tggaatatat cccttctaag ggcaatttta ttactattga
      961 tttcaaacgt cctgccgctc cgatttatga ggcattactg cgtgaaggcg tgattgttcg
     1021 ctcgatagca ggttatggaa tgccgaatca tttacgcatt agtatcggtt taccgcaaga
     1081 aaacgagaga ttttttactg ccttattgaa agtgttagct taacaagcgg ttacctttta
     1141 tgaaaatttt acaaatttaa agagaaaaat ggaaaaacta actttaaccc cgatttcccg
     1201 agtagaaggc gagatcaatt tacctggttc taaaagcctg tctaaccgag ccttattatt
     1261 agccgcctta gccaccggta cgactcaagt gaccaattta ttagatagtg atgatattcg
     1321 acatatgctc aatgccttaa aagcgttagg cgtgaaatat gagctatcgg acgataaaac
     1381 cgtctgtgta gttgaaggga ttggtggagc ttttaaggtt caaaacggct tatcactgtt
     1441 tctcggcaat gcaggcacgg caatgcgacc acttgcagca gcattgtgtt taaaaggtga
     1501 ggaaaaatcc caaatcattc ttaccggtga accaagaatg aaagaacgcc cgattaaaca
     1561 cttagtcgat gctttacgcc aagtaggggc agaggtacag tatttagaaa atgaaggcta
     1621 tccaccgttg gcaattagca atagcggttt gcagggcgga aaagtgcaaa ttgacggctc
     1681 gatttccagc caatttctaa ccgcattgct gatgtctgcc ccattagcgg aaggcgatat
     1741 ggaaattgag attatcggtg atctggtatc aaaaccttat attgatatta ccctttcgat
     1801 gatgaacgat tttggtatta cggttgaaaa tcgagattac aaaacctttt tagttaaagg
     1861 taaacaaggc tatgttgctc cacaaggtaa ttatttggtg gagggagatg cctcttctgc
     1921 ctcttatttc ttagcctccg gtgcgattaa aggcaaggta aaagtaacgg gcattggtaa
     1981 aaaatcgatc caaggcgacc gcttgtttgc cgatgtgttg gaaaaaatgg gggcaaaaat
     2041 cacttgggga gaggatttta ttcaagccga gcaatccccg ctaaaaggcg tagatatgga
     2101 tatgaatcat attcctgatg cggcaatgac gattgcaaca accgctttat ttgccgaagg
     2161 agaaacagtt atccgcaata tttataactg gcgggtaaaa gaaaccgacc gcttgacagc
     2221 aatggcaacc gaattgcgta aagtcggggc agaggtagaa gaaggggaag attttattcg
     2281 gattcaaccg cttgcgttag aaaacttcca gcacgctgaa attgaaacct ataacgatca
     2341 ccgtatggca atgtgttttt cattaattgc gttatcgaat acagaagtga cgatcttaga
     2401 tccaaattgt accgctaaaa cgttcccgac ttactttagg gagttggaaa aattatcggt
     2461 cagataaaag taaaaaagga ttcagaaaac tgaatccttt ttacgtttta ttgtggcaga
     2521 ctaagcccaa ccgctgttgt tacgtctgcg gcgagggatc aggaaaggca cgagtaaccc
     2581 aagtagtaaa cctgcgccta acacagcacc accatagatg aaccactgaa tcacgatttc
     2641 acgtttctcg gaatcgtgca ttgtttctaa atcacgattt ttgtttttca ggatttccag
     2701 ttcacgtttt agttgtgcat tttcctcaac caaatcactg cttttctgca ctgaatcttg
     2761 cgttctacgt tgcatttctg cggtacgttg ttgccattcg gtatcgattt tgctcaggcg
     2821 attggttaaa tcttgaacct gttgttttaa ttgaggaatc agctcttttg gactagcagt
     2881 atcgcttaag tctgaattaa gtacccaacc ttcacggtta cgactatctc gaatcagact
     2941 gtagcgctct ttacgatcta atactgtgac tttttcacct gcttgaatag cgcctgaaat
     3001 gcggaattga tcaccagcac ctttgcgcat aaatgtactc agattttcag taatgtaagt
     3061 gtctgccgtt aaagcagggc tagataggcc gagtaaaaaa caagacagaa gagctgatga
     3121 taatttcttc atatatattc ctct
//
LOCUS       DANLXQ010000213         3076 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.217, whole genome shotgun sequence.
ACCESSION   DANLXQ010000213 DANLXQ010000000
VERSION     DANLXQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.217"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(141..1736)
                     /locus_tag="PXD94_002498"
     CDS             complement(141..1736)
                     /locus_tag="PXD94_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL6287303.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            1885..2325
                     /gene="mioC"
                     /locus_tag="PXD94_002499"
     CDS             1885..2325
                     /gene="mioC"
                     /locus_tag="PXD94_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL6287304.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKTNLIIVTSTHGAGELPENIQQLFDDLANSSADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFVEQL"
     gene            2337..2504
                     /locus_tag="PXD94_002500"
     CDS             2337..2504
                     /locus_tag="PXD94_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL6287305.1"
                     /translation="MNKISANKAKWIKIAIILIYMFSPVDILPEAILGPFGLVDDAAA
                     LMLLIKTILEK"
     gene            complement(2593..3066)
                     /gene="asnC"
                     /locus_tag="PXD94_002501"
     CDS             complement(2593..3066)
                     /gene="asnC"
                     /locus_tag="PXD94_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDL6287306.1"
                     /translation="MYSKNTTKSEIDKLDQKILRWLIKDARTPYAEMAKVFGVSAATI
                     HVRVEKMRQAGIIESTKIVVDERKVGYDVCCFIGIILKSAKDYDKVIAKLSEFNEVVE
                     AYYTTGNYSIFIKVMTHTIEELHSVLAGKIQSIDEIQSTETLISLQNPISRDILP"
BASE COUNT          911 a          642 c          603 g          920 t
ORIGIN      
        1 cttttgtgat tacggctatg caaaatgatt tgcaaaacta tgcaaaataa atcgaccgct
       61 tacaattcta aaagaaaagc agtctattct ctaccacatt ttgcaaaaaa gtggcaaaaa
      121 tagaccgctt gtatggcata tcactccgat aaactcaccc cataaaatgg cgtgtagcca
      181 aacggatttt gcacaaaacc ttgcacccgt ttgctgagtg gtacataatt gatagcgtga
      241 gccagcggaa taaatggcgt ttgttcgttg gcaatttcca ccgcttgttg atagagcttc
      301 gcccgctcgt cacggttttg ggtttgtaca gcttgagcta ataactgctc aaatttctca
      361 tccgtccaac gcccataatt tgttcccgga atattagatt tgcttaataa agggaagagg
      421 aaattatcag gatcaccatt acggcttgtc caaccataag tgccggtaga gaactcgcct
      481 tcacgggtgc gtttgttaaa atccgcccat tcgtgggtca ctaatttcgc tttcaccccg
      541 attttcgccc aatccgcctg aataatttca gccgttcgac gtgggtttgg gttagaaggg
      601 cgcactaccg gttgtaccca aatttccgtt tcaaatccat cggcaaagcc ggcttctgtt
      661 aatagggctt tggctttttc aaggttaaat tcatattgcg gtaaatttgg attatagccc
      721 aacaccgaat cagggaacgg attacttgcc accgtgccac cgccttgata aacagcctcg
      781 acaatcgctt tcttatcggt tgcataatgc aaggcttggc gaactttggc gttatttaat
      841 gccggtttgg tgctgtttaa gccgatataa gctaaattca gcccgtctct ctgtaacaaa
      901 ttcaccttcg gatcttgttt catttgggca atatcggaaa tattcgggaa atcaatcaca
      961 tcgcattcac ccgctttcag tttggcataa cgagtgcccg catcaggggt aatactaaaa
     1021 atcagtttct caaattttgc tttaccttgg aaataatccg ggttcgcaac ataacgcacc
     1081 gcttgatcgg tttgataggc ttggaacaca aaaggccctg tgccgatcgg ggcttgatcc
     1141 aataactccg gcttgccttc cgccagcaat ttatccgcat attcttttga ataaatcgac
     1201 aagaaatcca tcgctaaggt tgtcagaaaa ggagcattag gttgctgtaa ggtgatttta
     1261 acagtgtaat cgtccacttt ttccactgtt tttaaaatct tcggcaaatt catccaatgg
     1321 taatagaaat aagtaccgtc agagacctta tggtaagggt ggtttaaatc catctggcgt
     1381 tggaacgaga acactacatc atccgcattc agcgttcgag ttggcgtaaa ttgtttattg
     1441 gaatgaaatt tcacgtcttt acgtaaatgg aatgtgtaac ttaagccatc gtcactgatt
     1501 tcccaacgtt ccgccaaact cggctcaacc gttagttcac cggctttaaa cgctaataag
     1561 cgattataaa tttgttgtga actggcgttg aaatcattgg catcattagt aattgcagga
     1621 ctaagtttca tcggtggagt tgccacgcaa ttaatcaacg tatttaccgg agctgctgcc
     1681 accaaactgc tggcacttaa gagtaaaaca gaaagagata attgagaaag acgcataaag
     1741 actccttaac attttctact aacttactcg gcattaacgt attttcaaaa taactaaagg
     1801 ttataattta taatcagaat aatcagttgc aaaccaatga ttcttggcta gaatattggc
     1861 agttttttaa acgagagatt tactatgaca tcaatttgta tcatcaccgg cagcacctta
     1921 ggcggtgcgg aatatgttgc cgatcacatt aatgaggtat taagcgaaca aggttttgaa
     1981 gtggaactgt ttaaccaagc cacccttgcc gatattgagg gcaaaaccaa tctgattatc
     2041 gttacctcta cccacggagc cggcgagtta cccgaaaata tccaacagct atttgacgat
     2101 ttagcaaata gctcggctga tttcagcagt atgaaatttg gtgtaatcgg tttaggtagc
     2161 tccgactatg acaccttctg caatgcagta aatattgttg aagaagctct caaagcgaaa
     2221 ggggcaagcc aagtttgcga atcactgaga attgatgtaa tgaataattt cgaccacgat
     2281 ggcagtgctg aagaatggtt accatcattt gttgaacaat tataacttaa gtatcaatga
     2341 acaaaatcag tgcaaataag gcaaagtgga ttaaaatcgc tattatctta atctatatgt
     2401 tcagccctgt agatattcta cccgaagcga ttttaggccc attcggtttg gttgatgatg
     2461 ccgccgcatt aatgctattg attaagacta ttttggaaaa ataggcatag tagacaaaat
     2521 agttgcaaaa aaccaaaaca aaacgaccgc ttgtaaccca atacaagtgg tcttatcttt
     2581 tacttaattc ttttaaggga gaatatctct tgaaatagga ttttgaagtg aaataagcgt
     2641 ttctgttgac tgaatttcat caatagactg aatttttcct gctaataccg aatgcaactc
     2701 ttcgatggta tgggtcatta ctttaataaa aatagaataa ttaccagtgg tatagtacgc
     2761 ctcaacaact tcattaaatt cactaagttt agcaattact ttatcataat ctttggctga
     2821 ttttaaaata atgccaataa agcaacacac gtcataccca accttacgct cgtccactac
     2881 gattttggta gattcaataa tgccggcttg gcgcattttt tcaacacgga catggatagt
     2941 tgcggcactc acaccaaaaa cttttgccat ttccgcataa ggcgtgcgag catctttaat
     3001 tagccaacgt aaaatttttt gatccagctt atcaatttcc gatttagtcg tgtttttact
     3061 gtacattttt tctcct
//
LOCUS       DANLXQ010000214         3040 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.218, whole genome shotgun sequence.
ACCESSION   DANLXQ010000214 DANLXQ010000000
VERSION     DANLXQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.218"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            49..2337
                     /locus_tag="PXD94_002502"
     CDS             49..2337
                     /locus_tag="PXD94_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDL6287307.1"
                     /translation="MEEHTIQSFYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNYPNLTALHQQFNQFI
                     AQFRERIATQTPNTQEAQRKAEVNRLRSEILDYAVSQAKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGTEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDTPVVVTTAVQFFESLFADRSSRCRKLHNIVGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLESEKLVL"
     gene            2468..>3040
                     /locus_tag="PXD94_002503"
     CDS             2468..>3040
                     /locus_tag="PXD94_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287308.1"
                     /translation="MREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLPGMGALA
                     DRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKITAEYVLS
                     EEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYENWGIDSM
                     MVGRYWGKHNPDLSRKLQIRDIRVKGKCPKL"
BASE COUNT          924 a          579 c          674 g          863 t
ORIGIN      
        1 tagaggtagt attgcttgtg ttaggaagga aatattgaag gtataaaaat ggaagaacat
       61 actattcagt ctttttatgc ccattcgggg aagctttcag atcattcaga ttggcaaact
      121 ttactcagcc acgccaataa tgttggcttg cttgccgccg aatttgctgc gccgtttgct
      181 tcacaagaaa ttgctcgctg cacggggcta ttgcacgatt taggcaaata tagtgaacgt
      241 taccaaaagc gtttacatgg cggagaacgg gtaaatcact ctactgctgg tgcaaaaatt
      301 gcagtggaac gttgggggcc tttgggcaaa atgatggcgt tttgtatcgc aggccaccac
      361 gctgggttag caaatggttc tggagaaggg aaaaatcgtt ctacacttac acaacgtttg
      421 caagagcaat ttggctatgg tcataaagat ctaccggtac ttgacgctat ttggcagcaa
      481 gaacttaatt tgcctgaaaa attgccctta ccgccgttaa agccttctaa aaacgaacct
      541 ttcttttctt acgccttttt tatccgaatg ctctattctt gccttgtcga tgccgacttt
      601 cttgatacag aagcttttta tgccaatctc gaaaaaagac cgcttgaacg aggaaattac
      661 cccaatttaa ccgcactcca tcagcaattt aatcaattta ttgcacagtt tagggagcga
      721 attgccactc aaacgcctaa tacgcaggaa gcacaacgta aagcggaagt aaaccgcttg
      781 cggagtgaaa ttttggatta tgccgtatct caagcaaaac aggaaacagg tctgttttct
      841 ctgactgtgc caacgggcgg tggtaaaacc tttacttcaa tggcgtttgc ccttgagcat
      901 gctaaacagt atggtttatg ccgcattatt tatgttattc cgtttaccag cattattgaa
      961 caaaatgctg cggaatttcg taaagctttt ggcgaattag gaacagaggc ggtattggaa
     1021 catcacagca cttttgatca cgatagccta cctgataaat caagccgaga taagctgaaa
     1081 ttggcatcag aaaattggga tactcctgtt gtggttacaa ctgcagtgca attctttgaa
     1141 agtttgtttg ccgaccgttc ttcacgctgt cgcaaattgc ataatatcgt cggtagcgtg
     1201 attattttgg acgaagccca aatgctaccg ttgaatttat tacgcccaat tatggctgcg
     1261 attgatgaat tagctcgcaa ttatcaatgt tcggtagtgc tttgcacagc gacacaaccc
     1321 gctattcatc aagcgaacgg tttttataaa ggatttgaaa atgtgcgaga aattgcccct
     1381 aatccaaccg cactttttga aaaattgaaa cgtactacgg taaagcatat cggtattcaa
     1441 acagatgtgg agttgcaaag caaactcgca gataacccac aaatgctgat aatcgtgaat
     1501 aaccgccgtc acgcccgagc gttatatgat tcagcgaaac agttagaggg agcttatcac
     1561 ctcaccacgc taatgtgtgc taaacaccgc tcacaaacgc tgactgaaat tcgtcttcgc
     1621 ctaaaacaaa acctaccttg ccgagtgatt gccacttctt taattgaagc cggtgttgat
     1681 gtcgatttcc cactggttat gcgagctgaa gcaggactag attcagtggc tcaagcagca
     1741 gggcgttgca accgagaggg gaaacgttta tctgtagaaa gcaatgtttg ggttttccaa
     1801 cctgaagaac agtggaaagc accaccagag ctaggcttac tttctggctg tatgcgttca
     1861 acggttcagg ttcacgcaga tgatctactt aatccgaaag caatcaatca ttatttccaa
     1921 gcggtttatg aggctaaagg aaaagagctg gataagccaa atattttaaa ggcgtgtcac
     1981 gacgctgggc aaagccttaa tttcccattc caaaaaatag ctgaagattt ccgaatgatc
     2041 acaagccatt taattccggt gattatccct ttcgaccaag aagccgaaaa ccttattgac
     2101 cgtttacgct atgcggatca agtaggcggt attttaagaa aactccagcc ttacactgtg
     2161 caagtaccgg aaaaagcatt agccgaactc tataacgctg gcagagttga aaccattaat
     2221 gaaaccaact tcggcaaaca gttttactgc ttaattggaa tggatttgta tgatgaagtg
     2281 gcggggttga gttgggagaa tgtggatttt cttgagtcgg agaaattggt tttatagatg
     2341 aatgtttatt ttaacttctt tataagttta attttgcata gatatacttt aatataccaa
     2401 atagcctatg acgtctcgta gtaaacagct agtttgtaag aatagatatt tggaagggaa
     2461 acgtaaaatg agagaaccat tattagatga agatgaatta aaaaaatggt tgcaaactat
     2521 ttttactgac aatttggtca ttattgtcgg ttcgggtttg tcctgtgccg aaggtttgcc
     2581 gggaatggga gcattagctg atagattaaa agaacgaatg cctgaatgtc ttgatgatat
     2641 agataaggta acatggaata ccatttctga ttgtctggat tctgaaggct tggaaggtgc
     2701 attgttgaag catcaagcaa atgagacgat agaagccgct attattaaaa taacagccga
     2761 gtatgtactc tccgaagagc aaaaagcgat aaacaaatgt attgctgaga atcagaagct
     2821 caaattttct tatttgcttc cccatatttc agcttcaaat cctaaaattg cccgtgtaat
     2881 tactactaat tatgatagat tgattgagtt tgcggcagag tatgaaaatt ggggaataga
     2941 ttcaatgatg gtcggtcgtt attggggaaa acataatcct gatttgagta ggaaattgca
     3001 gataagagac attagagtaa aaggtaaatg tccaaaattg
//
LOCUS       DANLXQ010000215         2979 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.219, whole genome shotgun sequence.
ACCESSION   DANLXQ010000215 DANLXQ010000000
VERSION     DANLXQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.219"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            160..891
                     /gene="rsmE"
                     /locus_tag="PXD94_002504"
     CDS             160..891
                     /gene="rsmE"
                     /locus_tag="PXD94_002504"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL6287309.1"
                     /translation="MRIPRIYQPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
     gene            891..1451
                     /locus_tag="PXD94_002505"
     CDS             891..1451
                     /locus_tag="PXD94_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL6287310.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            1451..1876
                     /gene="ruvX"
                     /locus_tag="PXD94_002506"
     CDS             1451..1876
                     /gene="ruvX"
                     /locus_tag="PXD94_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL6287311.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            1923..2975
                     /locus_tag="PXD94_002507"
     CDS             1923..2975
                     /locus_tag="PXD94_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL6287312.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVGMLLSVPLTMACKVALESNKATQKYA
                     VLFSDLEEVPKEQKYR"
BASE COUNT          903 a          572 c          634 g          870 t
ORIGIN      
        1 gagagaacta tatcataacg gctatagaat agtgtgtgaa gttaaagaga atgaaatttc
       61 gattattact attgtgcatt gttcaagact ttatccttaa cccgttttaa cttacctatg
      121 gcagacaatc gtctgccttt aatttataac tagataatta tgagaatccc aagaatttac
      181 caaccacagc cgttagctgg tcttcaaagt tgcgtgttaa gtgaagatgc cgctaaccac
      241 gttgggcgag tgctacgaat gaaacaagga gagcaaatca ttttatttga tggttcaaac
      301 cacgttttcc acgctacgtt acaagcggtc gaaaaaaagc aaattattgc aaaaattgat
      361 tcaagtgaat tagatgaccg agaatccaac ctgccgatcc atttagggca agtgatttcc
      421 cgaggcgata gaatggaatt taccattcaa aaatcggtgg agctgggggt aaaaactatt
      481 accccacttt ggtcggaacg ttgtggcgtg aaactcaatg atgaacggca agataaaaaa
      541 ttacagcaat ggcagaaaat tgcgattgcc gcctgtgaac aatgcggacg aaatgaaatc
      601 cctgaaattc gcccgattat gaaactcacc gactggtgca aagaacaaga cgatatgttg
      661 aaactgaatt tgcacccaag agccaaatat accattcgtg aactgccgaa tgtgccgaaa
      721 gctggtgttc gtttactgat tggctctgag ggcggattat ctgccgaaga aatcgccatg
      781 accgagagcg aaggttttac tgaagtatta ctgggtaaac gagtgttacg cacagaaacc
      841 gcctcacttg cggcaattac tgcgttacaa gtttgttttg gagatattta atgttagaga
      901 atttacaagg taaattttta attgccacgc cggaaatgga cgatgactat ttcgagcgta
      961 cggtggtcta tatttgcgaa cataatccgc aaggtgcaat gggagtagta attaacactc
     1021 ctaccgatct gtcggttcta gagctgatta cccgaatgga tttccaaatg gcgaaccagc
     1081 gtgattacaa aaaagaccaa atggtgttaa gcggtgggcc ggtaaatcaa gatcgtggct
     1141 ttattattca caccaaaacc gccaatgatt ttattcacac ttatccgatc accgataaaa
     1201 ttacgctcac tacctccggt gatgtgttag actcctttgg cacactggct tcacctgaaa
     1261 aatttattgt gtgtttaggt tgttccactt ggcgtggcag ccagcttgag caagaaattg
     1321 ctcaaaatta ttggattgtg tctgatgcga atgaaaaaac gctgtttgaa accggttatt
     1381 tagagcgttg ggcagaagcc tatgaaatga tggggctttc aagcataccg tcaagagctg
     1441 ggagagctta atgacagaaa ccacaacaaa aaccgtgatt gcctttgatt ttggcacaaa
     1501 tagcatcggc tgtgcagttg gacaaagtat taccggcaca gctcaaggtt taactacatt
     1561 taaagcccaa gacggtattc caaactggga agagatcgga aaactcttgc aagattggaa
     1621 accagatatg ctggttgttg ggctaccctt gaatatggac ggcacagagc agcctttaac
     1681 tcagcgtgcc agaaaatttg ccaatcgctt aaacggacgc tttaatctgc cggttgagct
     1741 acaagatgaa cgcctcacta ctgttgaagc aaaagcggaa attttttctc gtggtggtta
     1801 caaagcatta aaaaaggata aagtggattc catttccgct tgtttaattt tagaaagttg
     1861 gtttgagaat cactaatttg taaaaaattg ctgattttta accgcttgta aaaggaaaaa
     1921 ttatgacaca ttccaccaaa actctcgtgg cgatagcctc cattatcatt attctggcag
     1981 ggattaaatt atccaatgaa attgttgtgc catttttact ttcgctattt attgccatta
     2041 tttgttcacc aatgatcaaa tttatgacta agcgtaaagt gccattatgg ttagccatta
     2101 ccattttact tattgttttt atcttaggat tcagtttctt agcaggcatt attaataaca
     2161 ccttgcaaga atttactaat gctattccgc aatacaaaat tcagctttca tctagaatcc
     2221 aggtgattct agaactcgcc catagatggc atattccact taccctctca caagaagaga
     2281 ttatgagcaa atttgaccca agctcaataa tgaatttggt cagcaaactg ctaatggggc
     2341 tatccggtgc attatcgaat atttttgtga ttttcttagt cgtcattttt atgctattgg
     2401 aaatgccggt tgccaaatat aaattagccc tcgcacttag cccagatgat gatttaagca
     2461 acgaaaaaga gtatatcaac caagtacttg aaggggtgat tggttacctt ggagttaaaa
     2521 cctttgtgag cctactcacc ggagggttag tttggatctt actagaaatt ctaggcgtag
     2581 aatatgcgat tttatgggca attctaacct tcctgcttaa ctacgtcccg aatatcggct
     2641 ctattttggc gggaattcca attgtgattc aagccttggt actcaatggc ttttctatag
     2701 gattaggcac tttaatcggc attgtcgtga ttaatatggt tgtgggtaat gcgcttgaac
     2761 ctaaattaat gggcaagaaa ctcgggcttt ctacccttgt agtgttcctc tcacttctat
     2821 tttgggggtg gttacttggc accgtaggaa tgttactttc tgttccactt acgatggcgt
     2881 gtaaagtggc attagaatcc aataaagcca cccaaaaata tgccgtatta tttagcgatt
     2941 tagaagaagt accgaaagaa caaaaatatc gttaaattc
//
LOCUS       DANLXQ010000216         2957 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.220, whole genome shotgun sequence.
ACCESSION   DANLXQ010000216 DANLXQ010000000
VERSION     DANLXQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.220"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..528)
                     /locus_tag="PXD94_002508"
     CDS             complement(<1..528)
                     /locus_tag="PXD94_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDL6287313.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDH"
     gene            complement(619..1446)
                     /locus_tag="PXD94_002509"
     CDS             complement(619..1446)
                     /locus_tag="PXD94_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034033256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-2"
                     /protein_id="HDL6287314.1"
                     /translation="MAIRIFAILFSIFSLATFAHAQEGTLERSDWRKFFSEFQAKGTI
                     VVADERQADRAMLVFDPVRSKKRYSPASTFKIPHTLFALDAGAVRDEFQIFRWDGVNR
                     GFAGHNQDQDLRSAMRNSTVWVYELFAKEIGDDKARRYLKKIDYGNADPSTSNGDYWI
                     EGSLAISAQEQIAFLRKLYRNELPFRVEHQRLVKDLMIVEAGRNWILRAKTGWEGRMG
                     WWVGWVEWPTGSVFFALNIDTPNRMDDLFKREAIVRAILRSIEALPPNPAVNSDAAR"
     gene            complement(1523..2314)
                     /locus_tag="PXD94_002510"
     CDS             complement(1523..2314)
                     /locus_tag="PXD94_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDL6287315.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2372..2905)
                     /gene="aadB"
                     /locus_tag="PXD94_002511"
     CDS             complement(2372..2905)
                     /gene="aadB"
                     /locus_tag="PXD94_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HDL6287316.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
BASE COUNT          678 a          845 c          729 g          705 t
ORIGIN      
        1 atgatcgcat tcaatcacgt tgttccggta cttaatctgt cggtgttcaa cgtcagacgg
       61 gcaccggcct tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg ctttatccgt
      121 gttgatgaat cgtgggatct gccacttctt cacgttgttg aggattttac ccagaaaccg
      181 gtatgcagct ttgctgttac gacgggagga gagataaaaa tcgacagtgc ggccccggct
      241 gtcgacggcc cggtacagat acgcccagcg gccattgacc ttcacgtagg tttcatccat
      301 gtgccacggg caaagatcgg aagggttacg ccagtaccag cgcagccgtt tttccatttc
      361 aggcgcataa cgctgaaccc agcggtaaat cgtggagtga tcgacattca ctccgcgttc
      421 agccagcatc tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc
      481 ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat
      541 cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc ccgttgaagt
      601 aaccggcgct gcgcggtttt atcgcgcagc gtccgagttg actgccgggt tgggcggtaa
      661 cgcttcaata gagcgaagga ttgcccgcac gattgcctcc ctcttgaaaa gatcatccat
      721 tctgtttggc gtatcaatat tcagtgcgaa gaatacggag ccagtcggcc actcaaccca
      781 tcctacccac caacccatac ggccttccca gcccgtcttt gcacgcagta tccagttgcg
      841 accggcttcc acaatcatga gatccttgac caagcgctga tgttctaccc gaaagggcag
      901 ctcgttacga tagagcttcc tgagaaatgc aatttgctcc tgcgccgaga ttgcaaggct
      961 gccttctatc cagtaatcgc cattacttgt cgaaggatcg gcgttgccat agtcgatttt
     1021 cttcaaatag cgccgagctt tgtcatcacc aatttccttt gcaaatagct catacaccca
     1081 aacagtagaa ttccgcattg ctgatcgcaa atcttggtct tgattgtggc ctgcaaagcc
     1141 cctgttaacg ccgtcccatc gaaaaatctg gaactcatca cgaacagcgc ctgcatcaag
     1201 tgcaaaaagt gtatgaggta tcttgaatgt cgatgcaggc gagtagcgtt tcttcgatcg
     1261 cacaggatca aaaaccaaca tggcacgatc cgcttggcgt tcgtctgcca caactatcgt
     1321 gcctttggct tgaaattcgc tgaaaaactt cctccagtca gaacgttcta gcgtgccttc
     1381 ttgcgcatgc gcgaaagtgg caagagaaaa aatggagaaa agtatcgcga agattcggat
     1441 tgccattaac ttttccttaa tgcccaacgg ccaaggtaag ccgcgccgcg aagcggcgtc
     1501 ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt
     1561 agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
     1621 gataagcctg tctagcttca agtaagacgg gctgatactg ggcaggtagg cgttttattg
     1681 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tattgcgctg taccaaatgc
     1741 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
     1801 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
     1861 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
     1921 ccagatcaat gtcgatcatg gctggctcga agatacccgc aagaatgtca ttgcgctgcc
     1981 attctccaaa ttgcagctcg cgcttagccg gataacgcca cgggatgatg tcgtcatgca
     2041 cgacaagggt gacttctata gcgcggagcg tctcgctctc gccagggaaa gccgaagcct
     2101 ccataagatc attgagcaat gctcgccgcg tcgtttcatc aagctttacg gccacagtaa
     2161 ccaacaaatc aatatcgctg tatggcttca ggccgccatc cactgcggag ccgtacaaat
     2221 gcacggccag caacgttgat tccagatggc gctcaatgac gcttagcacc tctgatagtt
     2281 ggttcgaaat ttcgatggtc accgcttccc tcatgatgtc taacacctga gttaagccgc
     2341 gccgcgaagc ggcgtcggct tggacgaatt gttaggccgc atatcgcgac ctgaaagcgg
     2401 cacgcaagac ctcaaccttt tccgccccga gtgaggtgca tgcgagcctg taggactcta
     2461 tgtgctttgt aggccagtcc actggtggta cttcatcggc atagtaaaag taatcccaga
     2521 tgatcgcctc ccagctgtta caacggactg gccgcccggc gatgacgccc tcagccgcct
     2581 ctgggcacga gccctgcgga gcctccgcga tttcatacgc ttcgtctgcc caccaagcag
     2641 gttcgcagtc aagtaactca tccccgatct ccgctaagaa tccatagtcc aactcctcca
     2701 tgacgcgccc gccgagcatt tcaactattg cctcgagctc gccgcgcctc tcgccgggaa
     2761 acgtcagatc aatatcatcg tgcttgcgtg ttacacgccc tagccgtgca tcgatcgccc
     2821 agcccccacc gatccagagc ggcagatttc gctcatctgc cgcagctaga attttgtgta
     2881 tcaatgtgac ctgcgttgtg tccatgcggc ctaacctttg ttttagggcg actgccctgc
     2941 tgcgtaacat cgttgct
//
LOCUS       DANLXQ010000217         2913 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.221, whole genome shotgun sequence.
ACCESSION   DANLXQ010000217 DANLXQ010000000
VERSION     DANLXQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.221"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..563
                     /locus_tag="PXD94_002512"
     CDS             <1..563
                     /locus_tag="PXD94_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287317.1"
                     /translation="SVISIALYFKWKYILPLIAFTALILFLAIVFPENRYVEHALEPV
                     INLLSGTGKVSSSTDNLVKNHLFLPTLNQFISGDGLYYTETGGYYGGSDSGYIRQVLY
                     GGVSYMLACFAFTYYFVRKVAVNWFEGSWIFTLSTIGILAILNIKADTFAFPGLMMLF
                     LMFLSLFGTSGRNVTFFCKNKEAEYV"
     gene            556..1521
                     /locus_tag="PXD94_002513"
     CDS             556..1521
                     /locus_tag="PXD94_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL6287318.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDDCSKIPVS
                     ETLGILNEFNDISTKEKVRELLTRDSLSHIYSYPFDVKVVRNNPNVGAAQSRNVGATN
                     ATQEWLLFLDDDDRFMPEKCERLAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKH
                     KSELTKENILKANKIGGMPMIGLKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEP
                     LYVDEALTYCTFHTKRSSVSTDTTNTEKAIEYIQQTYIKTPEANNFKLNSLYILSYPH
                     VMNLSRKAAGYYFEMFKQTKNIKQLIIALVIFISPKLAINLKRFM"
     gene            1521..2321
                     /locus_tag="PXD94_002514"
     CDS             1521..2321
                     /locus_tag="PXD94_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6287319.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGAVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTTVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(2358..2864)
                     /gene="msrA"
                     /locus_tag="PXD94_002515"
     CDS             complement(2358..2864)
                     /gene="msrA"
                     /locus_tag="PXD94_002515"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL6287320.1"
                     /translation="MQNIREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSY
                     QEVCAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYT
                     DPQDEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQL
                     MNEILKNQ"
BASE COUNT          863 a          510 c          605 g          935 t
ORIGIN      
        1 tgtcagtaat aagtattgca ctctatttta aatggaaata tatattgcca ctgattgcct
       61 tcactgcgtt aattctattt ttggcgattg tttttccgga aaaccgttat gttgaacacg
      121 ccttagaacc ggtaattaat ttattgtccg gcaccggaaa agtcagctct tcaaccgata
      181 atttagtgaa aaaccatctg tttttaccga ctttaaatca gtttatttcc ggtgatggtt
      241 tatattacac cgaaactggt ggctattatg gtggatcgga ttccggctat attcgccaag
      301 tattatatgg tggagtaagt tatatgttgg cgtgttttgc cttcacttac tattttgtac
      361 gcaaagtggc ggtgaattgg tttgaaggca gttggatttt taccttatcc accatcggca
      421 ttttagctat tttaaatatt aaagcagata cctttgcttt cccggggctg atgatgctgt
      481 ttctaatgtt cttgtcgctg tttggaacaa gcggtcgaaa tgtaacattt ttttgcaaaa
      541 acaaagaggc tgaatatgtt tagtattatt gtgccttctt ataaccgtaa tcaagaaatc
      601 aatgcgttgc ttgagagttt aaagcaacaa actgcatata attttgaagt gatcattgtt
      661 gatgactgct caaaaatacc ggtcagtgaa actttaggaa ttttaaatga atttaacgat
      721 atttctacta aagaaaaagt aagagaatta cttactagag atagtttatc tcatatttat
      781 agctatccat ttgatgtgaa agtggtgcga aataacccaa atgttggggc ggcacaaagc
      841 cgaaatgtag gggcaactaa tgccacacaa gaatggctac tgtttttaga tgatgatgac
      901 cgctttatgc ctgaaaaatg cgaacgttta gcggcagaga ttgcgaacaa tccaactgcc
      961 aattttgtgt accacccggc aaaatgtgaa atggtaaatg agggttttac ttatgtcacc
     1021 aagccgttta aacataaaag tgagctaact aaagaaaaca ttctaaaagc gaataaaatt
     1081 ggcggaatgc cgatgattgg cttgaaaaaa tcactctttt gtaaaattgg tggattatct
     1141 gaccgcttgc gatcacttga agattatgat ttcttactga aattattgga agaaccgagt
     1201 tttgagccat tgtatgtgga cgaagccttg acctattgta ctttccatac caaacgctcc
     1261 agtgtttcaa ccgataccac taatacagaa aaggctattg agtatattca acaaacgtat
     1321 atcaaaacac ctgaggcgaa taattttaag ctcaattcgc tttatatttt aagttatcca
     1381 cacgtaatga atttatcccg caaggcagcc ggttattatt ttgagatgtt taaacagact
     1441 aaaaatatca aacaactgat tattgcgttg gttattttta tttcgccgaa gttggcgatt
     1501 aatttgaaaa ggtttatgta atgaaatttt ccgttttaat gtcgttatac attaaagagc
     1561 aaccgcaata tttaagagaa tgttttgaaa gcttgaaagt acaaactgtg caggctgatg
     1621 aaatcgttgt ttgttttgat ggtgcagtta cgcctgaatt agaggcgatt gtagaagaat
     1681 atagtgcaat tttgccgatt aatgcggtga aattcccaca aaatcgaggt ttaggtaaaa
     1741 cgctaaatga tgggctaacc tattgtaaga atgaatgggt tttcagaatg gatacagacg
     1801 atatttgtat gccggaacgt ttcgagaaac aagtggcatt tattgaacag aatcctgaaa
     1861 ccgtgatttt tggcggccaa attgctgaat ttgggcaaga tattcacgat cttgtcgctt
     1921 accgcaatgt accaaccacg gtggaggaaa tcgtcagatt cactcgcttg cgttgcccgt
     1981 ttaaccatat gacggttgct tatcagaaat ccaaagtgtt ggaagtcggt ggctatcaag
     2041 atctgcaaga agattactat ttatggatca aactcattgc tcaatttaaa caagtggcaa
     2101 acttgccgga tttattagtg tatgcccgag tcggtaacgg aatggttggc agacgccgtg
     2161 gaatggccca agcagaggca gaatggcgtt tatataaatt aaaaaaccgc tgccagattc
     2221 acgatccgat ttcaggtttt gggattttca taatgcgtgc aattcctcgt ttattaccgg
     2281 tttcagcact gaaaaaagtg tacggtttgc tcagaaaata aagctaaaaa aaccacagat
     2341 tgctctgtgg ttttttgtta ttgattcttc aaaatctcat tcattaattg aatatcaata
     2401 tggcagtagc cgttcgggtt ttttactaaa tattcttggt ggtactcttc ggcagggaaa
     2461 tattgctcta acatttggtt ttccactgcc agcggctgat caaattgggt ttgtaattgt
     2521 gcaagtgcgg cggttaccac cggttcgtct tgtgggtcgg tataataaat accggtgcgg
     2581 tattgaatac cgatgtcgcc accttgtttg ttaatgctca ccgggtctat tactttaaag
     2641 aagtaatcta gtaattttgc caagctaatt tgattggcat cataagtcac ttttaccact
     2701 tcagcgtgtc cgctaccggc acacacctct tgatagcttg ggttatcggt attaccgttg
     2761 gcatagcctg attgtgcctt aatgacacct ttaatccgtt gcataaaaga ttccacgccc
     2821 cagaaacagc caccggctaa gtaaatttca cgaatatttt gcatattacc tctctataat
     2881 tgtaaatttt tcgataaatt tgactgcttg tga
//
LOCUS       DANLXQ010000218         2876 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.222, whole genome shotgun sequence.
ACCESSION   DANLXQ010000218 DANLXQ010000000
VERSION     DANLXQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.222"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(26..101)
                     /locus_tag="PXD94_002516"
     tRNA            complement(26..101)
                     /locus_tag="PXD94_002516"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Ile,seq:cat)
     gene            complement(195..932)
                     /gene="pgeF"
                     /locus_tag="PXD94_002517"
     CDS             complement(195..932)
                     /gene="pgeF"
                     /locus_tag="PXD94_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL6287321.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEALIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            complement(964..1350)
                     /locus_tag="PXD94_002518"
     CDS             complement(964..1350)
                     /locus_tag="PXD94_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL6287322.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
     gene            1431..2432
                     /gene="yejK"
                     /locus_tag="PXD94_002519"
     CDS             1431..2432
                     /gene="yejK"
                     /locus_tag="PXD94_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL6287323.1"
                     /translation="MSINVSEIVLHQLQKTEGENPELNTFLRENLLAISPEVDQMMLQ
                     LHQAYQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSHSCAKMLAAELIKYPFADG
                     GTFILCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQYLEIAQYDIACRINLTELK
                     LDATSNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQNQTLLQAVSDYCEQGELSA
                     PQTREVKKQVFEYCKGQINSGEEIALNELSNALPTLNEIDFAQFTQEQEYGLEENIPP
                     MRNALKTLTKFSGSGKGVTISFNAELLGERLLWDELNDTLTIKGLPANLRDQLERNK"
     gene            2499..2840
                     /gene="bamE"
                     /locus_tag="PXD94_002520"
     CDS             2499..2840
                     /gene="bamE"
                     /locus_tag="PXD94_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL6287324.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
BASE COUNT          844 a          541 c          645 g          846 t
ORIGIN      
        1 tgaaagacaa tatgcaacgc ttagatggtc cccccagccg gacttgaacc agcgaccaag
       61 cgattatgag tcgcctgctc taaccactga gctatggggg gatttgaaaa gtggttggaa
      121 ttatagagaa aaaccgagta gtcgtctaga ggcagaagat tactcggtta attattgaac
      181 gagagaaaat agggttattc tgttcgccaa attaaggttg ccattcgccc ggtttgtttg
      241 tctcggcggt aggaaaaaaa cagttctggt tggctgtagg tgcaatattc accgcctgaa
      301 atggcggtaa tgcctaattt attcagacgt tgggtggcaa tttggtaaag atttcctaaa
      361 aatttaccgc ttgtagtgct gtcttcaata agggcttctt tcgctcttgg gtcaaaagca
      421 cagaattggt ctgccacttc actcccgact tgaaaagcat tcggtccgat agccggccct
      481 agccaagctg atatttcgtg tggcgggcag ttgaatttct ctaccgtcgc ctctaatatg
      541 ccatcgcata acccacgcca gccggcgtgt gccgccgcaa tttccgaccc gtcttggctg
      601 gcaaataata ccggcaaaca atctgcagtc atcactaagc agacttggtt tggttgattg
      661 gtgtaaatcg catcggcttt aatattatca cctgaatagg gaagttcaat cactcgggtg
      721 ctgtgagttt gggttaaaaa cagcggagct tgtggtaatt gaaatttctc aaccaataaa
      781 gcacgatttt gagcgatatc ttgtggctcg tcattaacgt gatcacctaa gttgaaactg
      841 tcaaaaggcg gtttgcttac tccgcctgtt cttaaggtcg tcagtgcgtg aatatgttta
      901 ggcacattcc aatttggaat aattttatcc atttgcaaaa aatctcaaga aaatgaccgc
      961 ttgttatcgg cgtactgcaa tcgcttcgat ctcaatgcca acatcttttg gtaaacgagc
     1021 cacttctacg caagaacgag ccggaaaatt agggtgttcg ttttcttgga agaaggcttg
     1081 gtattccgca tttacctttg cgaagtcgtt aagatctttg acaaatacgg tggtctttac
     1141 aatatcggca acggttaagc cggcttgttc gataatggct ttcacgttgt tcagagattg
     1201 gcgggtttgt tgcacaatat cactcgggat ttcgccggtt tcaggattca cgggaatttg
     1261 cccagaagtt aaaaccatat tgcctaaatc aactgcttgc acataaggcc caatcgctgc
     1321 cggtgcattt tctgtgtgga taactgtcat cttgtgttcc tcttaatgaa aaataaacgg
     1381 attaatgata atatcttttc ttattatttg gaataaagag aagtgaatta atgagcatta
     1441 atgtttctga aatcgtgtta catcaattac aaaaaacaga gggcgagaac ccggagctta
     1501 atacgttttt gcgagaaaat ttattggcaa tttcccctga agtcgatcaa atgatgttgc
     1561 aattacatca agcctatcaa agtaaagcaa aggcttacgg tatatttaaa ccggagtcgg
     1621 ttttcgctca acagctcaac cgcttgttgg agcaagaaac cgatttttta ccttttagcc
     1681 atagctgtgc caaaatgctt gcagcagagc tgattaaata tccgtttgcc gatggtggca
     1741 cttttatttt gtgtcgttac acttttttag ctaccgaata tttgtttatt gcgttgattg
     1801 atagccgtaa ttcaatgctg gttgatgagc agttagagat taaacgcacg caatatctcg
     1861 aaattgcaca atatgatatt gcctgccgca ttaatttaac tgaactgaaa cttgacgcaa
     1921 cgtcaaatcg ctatttaact tttgtgaaag gcagagtagg gcgtaaaatt gccgatttct
     1981 ttatggattt tttaagtgcg gaagaagggt ttaatccaca attgcaaaat caaaccttgt
     2041 tgcaagcggt gagcgattat tgtgagcaag gggagttatc tgcccctcaa actcgagaag
     2101 tgaaaaagca ggtgtttgaa tattgtaaag ggcagataaa tagtggagag gaaattgcgt
     2161 taaatgagct ttccaacgca ttgccaacac tgaatgaaat cgattttgcc caatttacgc
     2221 aagagcaaga atacggcttg gaagagaata ttccgcctat gcgaaatgcc cttaaaaccc
     2281 tgacgaaatt ttccggttcg ggcaaaggag taacgattag ctttaacgca gaattattag
     2341 gagagcgttt actttgggat gaactaaacg acacactgac aatcaaagga ttaccggcaa
     2401 atctgcgtga ccagctggaa cgtaacaaat aatggaaaat ttttatcagt ttaggtatta
     2461 tctcgttatc tgaattttgg attatcaaaa ggtcaattat gaaaatgaaa tctcttttag
     2521 ctgcaatgtt gcttgcactg ggtgtaacgg cttgttcaac cgttgagaaa gtggtgtacc
     2581 gtattgatgt accgcaaggc aattacttag aacaagataa aattgctcaa cttaaagtcg
     2641 gtatgacagc ggaacaagtt gaatatttgc ttggtacacc aatgttaaag aacatttttg
     2701 aaacccaccg ttgggattat gtgtttatta aacgtgaagg ttataaagat cctgaacaac
     2761 gcaatctgtt tatttatttt gatgctaaag ggttagtatc aaaaattgat gacagcaatg
     2821 cgaatgcaga acaaaaatag gacgatttgt tggttcgaaa agcccacttt taaaag
//
LOCUS       DANLXQ010000219         2871 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.223, whole genome shotgun sequence.
ACCESSION   DANLXQ010000219 DANLXQ010000000
VERSION     DANLXQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.223"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            36..2030
                     /gene="malQ"
                     /locus_tag="PXD94_002521"
     CDS             36..2030
                     /gene="malQ"
                     /locus_tag="PXD94_002521"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL6287325.1"
                     /translation="MKEYASPSAFEKIERLLNTDNDLQKNNQILPLVKVITQPNAEQQ
                     AVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPLGYHQLSISTENDEES
                     CSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDFGDLKQFLANISDYGA
                     DFVGLNPIHALFPADPDAASPYSPSFRLWQNIIYIDVSAVEAFQQSEQAQQWFHTKET
                     QQQLAELRAKDFVDYSQVAQLKLKALQFAFEHFSQSSQQQSQTAFDAFIEQAGEPLKI
                     QATFDALHHWISSEFGEKGGWTNWAEEYQSYYSPKVAEFQISQATQIRFYMWLQFVAN
                     QQLAECNRFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQEASIGAPPDMLAPNG
                     QSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGFARMWWVANGESAKEG
                     IYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQKGIFAYNVFYFEKAE
                     NAFKPLENYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFDTYPSKAVLRKLKEERSY
                     DRLNIRNAVEKVIALEPNEVGVTIKFTHQLQRYVAHTHSALFGTQPEDWLNMLEPVNI
                     PGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
     gene            complement(2098..2829)
                     /locus_tag="PXD94_002522"
     CDS             complement(2098..2829)
                     /locus_tag="PXD94_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL6287326.1"
                     /translation="MGCSSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTW
                     IATIAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQ
                     FIGFYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGS
                     ALAILDGATVVISIVAQILMVLRYREQWALWIIVNIMTISLWTAMYFQNGETSLPLLV
                     MYVMYLCNSIYGYYNWIKLHRKHQQ"
BASE COUNT          911 a          610 c          568 g          782 t
ORIGIN      
        1 aacaaaatac agctcactat ttttactccc caaatattaa agagtatgcc agcccctctg
       61 cttttgagaa aatagagcgg cttttaaata ccgataacga tttgcaaaaa aataatcaaa
      121 ttctaccgct tgttaaagtg attactcagc caaatgccga gcaacaagcg gtcgaaattt
      181 cgctgaattt tacaaatgta gcaaagtggc aaattgtgct tgaaaatggt gaggttcgga
      241 acggtgaaat taaggcagag caatcagttc taaccctgcc tgccgatttg cctttaggct
      301 atcatcagct ttctatttca actgaaaatg atgaagaatc ttgctcgctg attgtaacac
      361 cgccgaaagc tttccagcct aagccattgg aagaaaggaa aaagctctgg ggtgcatttt
      421 tacagcttta tactttgcgt tccgataaca actggggaat tggcgatttt ggcgatctca
      481 aacaattcct tgccaacatt tctgattatg gtgcagattt tgtgggctta aaccctattc
      541 acgccctctt tccggcagat cctgatgcgg caagccctta cagcccgtcc tttcgacttt
      601 ggcaaaatat tatttatatt gatgtttcag cagtagaagc ctttcaacaa tctgaacagg
      661 ctcaacaatg gtttcacacc aaagaaactc aacagcaact tgccgaattg cgagccaaag
      721 attttgtcga ttacagccaa gtagcgcaac tgaaattaaa ggctctgcag tttgcttttg
      781 agcatttttc acagtcatct caacaacaat cgcaaacagc gttcgatgcc tttattgagc
      841 aagcaggcga gccattaaaa atccaagcaa cgttcgatgc tttgcaccac tggatttcat
      901 ctgaatttgg cgaaaaaggc ggctggacaa actgggcgga ggaatatcaa agttactatt
      961 cgcctaaagt agctgaattt caaatatcgc aggcaacgca gatacgtttt tatatgtggt
     1021 tgcaatttgt ggcaaaccag caattagcag aatgcaaccg gtttgctcaa tcactttcaa
     1081 tgccgattgg tttttaccgc gatttagccg ttggtgttac tcaaagcgga gcggaaactt
     1141 gggcggataa agccctattt gtgcaagagg cgtcaatcgg tgcaccacct gatatgcttg
     1201 ccccgaacgg acagagctgg ggactttcgc caatgcaccc gcatattcta caaagccgag
     1261 gttatcagcc gtttattgat ttagtgcgtg ctaatatgaa acattgtggg gcattgcgaa
     1321 tcgaccatat tctcggcttc gcccgtatgt ggtgggtggc gaacggtgaa tcggcaaaag
     1381 aagggattta tgtccgttat ccgctcgaag atttgctctc tattttggca ttagaaagcc
     1441 aacgtcacca atgtttagtg attgcagagg cacttggcat tgtgcctgaa ggcattttgg
     1501 actctctcga gcaaaagggc atttttgcct acaatgtttt ttattttgaa aaggcagaaa
     1561 acgcgtttaa accgctggaa aattatcctt accaagcgat gacaactcta agcacccacg
     1621 atttaccgac tattcaaggc tattggaaag gctatgattt tgagctaggc gaaaaatttg
     1681 acacttaccc aagcaaagcc gtattgagaa agctgaaaga agagcgttcg tacgaccgct
     1741 tgaatatccg taatgcggtg gaaaaagtga tagcactcga gccaaatgaa gtgggagtaa
     1801 caataaaatt tacccaccaa ttacaacgct atgtggctca cactcacagt gcattattcg
     1861 gtacacagcc cgaagattgg ctcaatatgt tagagccggt gaacattccg ggaaccagca
     1921 ccgaataccc taactggaga agaaaattat ccgcaaccac cgagcagatt tttgcaaata
     1981 aagatattca acaactgcta aaagatataa acgaaattcg taaaggataa taacaagcgg
     2041 tcatttttag gtaaattttt acaaaaaatg accgcttgta atttgtattt attcgctcta
     2101 ttgctgatgt tttctgtgaa gtttaatcca gttgtaataa ccgtaaatag agttacataa
     2161 atacatcaca tacatcacca acagcggcaa gctggtttcg ccgttttgga agtacattgc
     2221 cgtccataaa gaaatggtca taatattcac gataatccac aatgcccatt gctcacggta
     2281 gcgaagtacc atcaaaattt gtgccacaat cgaaataacc actgttgcac catctaatat
     2341 agctaacgca ctgcctaaat atttaagcaa ttcaatataa agaaatgtgc cgataatggt
     2401 ggtaatggcg acaatcaccc actgttttgc ggttaaagct ttagcaatta cctcttccac
     2461 accagcatta tttaccgtgt tttcgctggt catattttta cgccagaaat agaaaccgat
     2521 aaactgcacc ggcacatata caagtaaatt cagcatcatt tcaccatata actgaaaggt
     2581 ataagagata taagcataaa gcgaaacgga aattaagccg aatagatagt tactgatttt
     2641 tcctttaccg acaaatacca cgcataaaat acctgttatt gccgcaatag ttgcaatcca
     2701 agtatcgggt tgataaataa aaataccgat ttgaatcgct aaaaaaacaa tcaaccaact
     2761 tgcctcaaaa gtagtccaac cactaaaaaa ctcttgtttt aacttagttg aaaaagatga
     2821 acagcccata tttcctccat ataaagtgaa gtattctttt caaattctaa g
//
LOCUS       DANLXQ010000220         2838 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.224, whole genome shotgun sequence.
ACCESSION   DANLXQ010000220 DANLXQ010000000
VERSION     DANLXQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.224"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            103..729
                     /gene="rlmE"
                     /locus_tag="PXD94_002523"
     CDS             103..729
                     /gene="rlmE"
                     /locus_tag="PXD94_002523"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL6287327.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRDRSREVYIVATGYKG"
     gene            850..2793
                     /gene="ftsH"
                     /locus_tag="PXD94_002524"
     CDS             850..2793
                     /gene="ftsH"
                     /locus_tag="PXD94_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL6287328.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVMMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
BASE COUNT          856 a          504 c          711 g          767 t
ORIGIN      
        1 ttaattaagc ataataagcc gagctaaatt ttagaacatc tcttgaattt gctaatttaa
       61 cccttattat aaatcgttga attaattaag atagaagaac aaatgggtag aaaacgaagt
      121 gcgagttcat cacgttggct tgcagaacat tttaaagatc aatttgtgca aaaagcacat
      181 aaacaaaaat tgcgttcacg ggcgtatttt aagttagatg aaattcaaca aaccgaccgt
      241 ttattcaaac cgggaatgac ggtggtggat ctcggtgccg caccgggcgg ttggtcgcaa
      301 tatgtggtga cgcaaatcgg cagtaagggc agagtaatcg cttgtgatat tttagatatg
      361 aacccgattg taggcgtgga tttcttacaa ggcgacttcc gtgaagaatc ggtgctgaat
      421 gcgttacttg agcgagtagg cgaagacaaa gtgaatgtgg tgatgtcgga tatggctccc
      481 aatttcagcg gtatgccgtc ggttgatatt cctcgtgcaa tgtatttagt ggaattggca
      541 ttggatatgt gtcgccaagt attagcaacc aaaggcagtt ttgtggttaa ggtattccaa
      601 ggcgaaggat ttgatgaata ccttcgagaa atccgctcgc tctttagtgt ggtaaaagtc
      661 agaaaaccgg aggcgtctcg agatcggtct cgagaagttt atattgtagc gacaggttat
      721 aaaggctaga aaacaagcaa gcggtcattt tttattaaca ttttgcaaaa aaattggaaa
      781 attcgaccgc ttttatagta atcttgaaca gttttttatt cagcagatag gggattaacc
      841 ttgaacgata tggttaaaaa tattttactt tgggtggtga ttgccattgt gatgatgaca
      901 gcctatgaag gttttaattc taatttcagt ggtagctcaa atacagtgcc atattcgact
      961 tttttaagcg atattaaaga aaatcgttta aaaggggtcg atttcaaaca gaatgatgac
     1021 accattttgg tgacgaaaaa tgatggtgca acctaccaaa ccgtaatgcc gatgtatgat
     1081 gagtacttaa tggcagattt agcaaaaacc aatgcagtga ttaccggtca gcctgctgag
     1141 cgtcgtggct tgctctcgca aattttgatt tcttggttcc caatgttgct gttaatcggt
     1201 ttctatgtgt tttatatgcg acaaatgcaa ggtggcggag gacgtggtgc aatgggcttt
     1261 gggaaaaata aagccaaaat gttgaccgca gacgaagtta agaccagatt ttctgatgta
     1321 gccggctgtg atgaagccaa agaagaagta ggcgaagttg ttgagttttt gcgtgaccca
     1381 actaaatttc aaaaattagg tgggcgcatt ccaaaaggga tcttgatggt aggccctccg
     1441 ggtacgggta aaaccttatt ggcgaaagcg attgcaggtg aagcacgagt gccattcttt
     1501 acaatggcag gttccgactt cgtggaaatg tttgtaggtg tgggtgcttc acgggtgcgt
     1561 gatttattcg accaagccaa gaaaaatgcc ccttgtatta tttttattga tgaaattgat
     1621 gcggtagggc gtaaacgtgg tggtgcaggt ttcagcggtg gacacgatga gcgtgaacaa
     1681 accctcaacc aaatgttagt tgagatggac ggctttgaag gctctgaagg tgtgattatc
     1741 atcgctgcaa ccaaccgtgc cgatgtgtta gatgatgcct taactcgtcc ggggcgtttc
     1801 gaccgccaag tcacggtcga tttgccgaac gtgaaagggc gtgagcaaat tttgaaagtt
     1861 cacttgaaaa aagtaccgct tgcagaggga gttgacccga tgcaaattgc tcgtggtacg
     1921 ccgggttact ctggtgctca attagcaaac ttggtgaacg aagctgcctt atttgctgcc
     1981 cgtaagaata aacgtgtggt aatgatggaa gatttcgaag aagcccgtga caaaatcaat
     2041 atggggccgg aacgccgttc aaatacaatg acggaaaaag agatcatcaa tactgcttac
     2101 cacgaagctg gacacgttat tgttggctat ttaatgccgg aacacgatcc gctgaataaa
     2161 gtgacaattg tgcctcgtgg tcaggcgtta ggttttgctc aattcttacc ggaaggtgac
     2221 cgtgtaagcg aaacatttac caaattggaa agccaacttt ccaccctatt tgcagggcga
     2281 attgctgagg gcttgatttt tggtgaagat aaaattacca caggggcttc ctctgatatt
     2341 catcgagcaa cccaaattgc acgagcaatg gttactcaat ggggcttctc aaaagaatta
     2401 ggcccgattt tctatcaaaa tgaagatgga atgggtgcaa ttaagggcgt atcggaagag
     2461 tcgcaaaaat tgattgatgt ggaaatgcgt aagatcattg accgcaacta tcaacgagcg
     2521 aagcaaactt tggaagaaaa tatggacatc ttacacgcaa tgaaagatgc cttattgaaa
     2581 tacgaaacct tagacagcaa gcaaattgat gatttaatgg cacgccgtcc ggtgggtgaa
     2641 ccatcaggtt ggacagatac cccaactaaa gacgataacg ccactccacc aagtggtgaa
     2701 caggcagcgg atgtaagttc aactttagag gataatccaa ataaagataa taacattggg
     2761 gattcgttag ccgataccaa agcggaacat taatctaatt gtttgctaga aagttagaat
     2821 aagaagaaat ctgtactc
//
LOCUS       DANLXQ010000221         2817 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.225, whole genome shotgun sequence.
ACCESSION   DANLXQ010000221 DANLXQ010000000
VERSION     DANLXQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.225"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1743
                     /locus_tag="PXD94_002525"
     CDS             <1..1743
                     /locus_tag="PXD94_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HDL6287329.1"
                     /translation="TIVIHPQDKLEAHLEKISSRAVGENFESKYLKEDCQTVQSYLKE
                     GHKLYQAAIAFYVRGKDEKELRTRSRQLRTILLSNNLEPVKENAEIAPLNSYLRWLPM
                     NFNPQMDAKSHYYTKYYFVQHLANLLPIFGRDTGTGNPGITYFNRGGAPFNFDPFNRK
                     DRTKNAHKLLLGPTGSGKSATLCGQLCQAMAVYRPRTFVVEAGNSFGLLADYAERFGL
                     TVNRITLAPNSGIVLPLFSEAHRLLDMELNPEDVPEDIEDNDDTDDEGDMQRDLLAEM
                     ELTARLMITGGEKKEDEKMSRADRAAIRRAILNAARQTKSEGRQTLTEDIKNALEALS
                     HDEHTREERRTRLAEMAESLEMFCTGVNGQFFNREGAIWPEADLTLVDLAMFAREGYE
                     AELAISYISLINHINSLGEKYQHSDRMILNVTDESHIITANPLLAKFLVKGSKMWRKL
                     GIWLWLATQNMDDFPNEAAKLLSMVEWWELLNVSASEIDEINRFRKLSEAQKTMLLSA
                     KKSDRKYTEGVVLATNMEALFRVVPPSLFLALGMTEKHEKAERKQLMMQHGYTELDAA
                     LEVAARIDKARGIH"
     gene            1743..2171
                     /locus_tag="PXD94_002526"
     CDS             1743..2171
                     /locus_tag="PXD94_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287330.1"
                     /translation="MNKQHYRLFNAGPQKSSLGVVSPAVETQLLGFCEQIRQLIGVGK
                     QITLFPHRVVQNHWAIELSGEQGSVHLLLNVTGRSLLPTSNNSEWSARLIIEIADHVD
                     AYWFSAFLTQYLGLKSNLLPLSTSIADNSVYNEVIDNNEV"
     gene            2161..2478
                     /locus_tag="PXD94_002527"
     CDS             2161..2478
                     /locus_tag="PXD94_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL6287331.1"
                     /translation="MKSDTTKPKMTRVITVLKITLMIIGIILLLLVVGWLAMTYFPST
                     NFQAWFYETRYAWLGWRFVLYVAIWLLMVQLNRKGQMPLKARLMILSVIPIIEGLNLL
                     YLL"
     gene            2489..>2817
                     /locus_tag="PXD94_002528"
     CDS             2489..>2817
                     /locus_tag="PXD94_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL6287332.1"
                     /translation="MTFTVDSYLEYFLTLLAWIINNNIFAVLVQTGLFVLPLIFVLIS
                     TWMEVKKQGEDEGNKGDLLITWLSLKFYPAMFVVVLVLAPMIPINLNNIELNVERSKA
                     CGYRVPTA"
BASE COUNT          850 a          530 c          617 g          820 t
ORIGIN      
        1 accatcgtca tccatccaca ggataagttg gaggcacatt tagaaaaaat tagtagtcga
       61 gcggtaggtg aaaattttga gtcgaaatac ctaaaagaag actgtcaaac tgtacagtct
      121 tatcttaaag aggggcataa actctatcaa gcagctattg cattttacgt tcgaggtaag
      181 gatgaaaagg agctacgtac ccgttctcgc cagttgagaa cgattctgct cagtaacaac
      241 ctagaacccg tgaaggaaaa tgcagaaatt gccccactta acagctattt gcgttggcta
      301 ccaatgaatt ttaatccgca gatggatgcc aaatcacatt actacacgaa gtactatttt
      361 gtacaacatt tggcaaactt attacctatc tttggccgag ataccgggac tgggaatccg
      421 gggatcactt actttaatcg aggaggagca ccatttaatt tcgacccatt taaccgtaaa
      481 gacagaacca aaaatgctca caagctatta ttaggtccaa ccggttcggg aaaatcagct
      541 acgttatgtg ggcaattatg tcaggctatg gccgtttatc gcccacggac ttttgttgtt
      601 gaggcaggaa attcatttgg tttattagcg gactatgcag aacgtttcgg actgactgta
      661 aatcgcatta cgttagctcc aaacagcggc attgtcttac cactcttttc agaagcacat
      721 cgtctattgg atatggaatt aaacccggaa gatgtgccgg aagacattga ggataacgat
      781 gacactgatg atgaaggtga tatgcaacga gatttattgg ccgaaatgga gctgacggca
      841 cgtttgatga tcaccggagg tgaaaagaaa gaagatgaaa aaatgagccg tgctgatcgt
      901 gctgctattc gtcgtgccat tttaaatgca gctcgccaaa ccaaatctga aggcagacaa
      961 acgctgactg aagatattaa aaatgctttg gaagcattaa gtcatgatga gcatactcgt
     1021 gaagaacggc gtacccgttt agcagaaatg gcagaatcat tagaaatgtt ttgtaccggt
     1081 gtaaatgggc aattttttaa tcgagagggg gcaatttggc cggaggctga tctaacctta
     1141 gttgatttag ctatgtttgc ccgggaaggc tatgaagcag agttggccat ttcctatatt
     1201 tcactcatta accatatcaa cagtcttggt gaaaaatacc aacacagtga tcggatgatt
     1261 ctgaatgtaa cagatgaatc acacattatc acggccaacc cattattagc taaattccta
     1321 gttaaaggct caaaaatgtg gcgtaaacta gggatttggc tatggcttgc tactcaaaat
     1381 atggatgact tcccgaatga agcggcgaaa ttactctcaa tggtcgaatg gtgggaattg
     1441 ttgaatgtgt ctgccagtga aattgatgaa atcaatcgtt tccggaaact gtcagaggcc
     1501 cagaaaacga tgttgttatc tgccaaaaaa tcagatcgta aatataccga gggcgtggta
     1561 ttagcaacga atatggaagc tctattccga gttgtaccgc ctagcttatt tttagcttta
     1621 ggaatgactg aaaaacatga aaaagcagaa cgtaagcaac tgatgatgca gcacggctat
     1681 accgaactcg atgcagcttt agaggttgca gctcggattg ataaagcaag aggaatacac
     1741 taatgaataa acaacattac cgtttattta atgcaggccc acaaaaatcc tcattaggag
     1801 ttgtatctcc agcggtagaa actcaattgc ttggattttg tgaacaaatt cgccaactca
     1861 tcggcgttgg gaaacagatt accttattcc ctcatcgtgt cgtgcaaaat cactgggcta
     1921 tcgaactttc tggtgagcag ggttctgttc acttattact taatgtgacg gggcgttctt
     1981 tgctaccaac ttccaataac agtgaatggt cagctcgttt aattattgaa atagctgatc
     2041 atgtggatgc ttattggttt tcggcattcc ttactcagta tctcggtttg aaaagtaatc
     2101 ttttaccctt atccacatca attgcggata actctgtgta taacgaggta atagataata
     2161 atgaagtctg atacaacaaa gcctaaaatg acaagggtta ttactgtatt aaaaataacc
     2221 ttgatgatca tcggtattat tttattacta ctcgttgttg gctggttggc aatgacctat
     2281 tttccttcca caaattttca agcgtggttc tatgaaaccc gctatgcctg gttgggatgg
     2341 cgatttgttt tatatgtagc tatttggcta ttgatggtac aacttaatcg aaaagggcaa
     2401 atgccattaa aagccaggtt aatgatttta tctgtcatcc caattattga gggacttaat
     2461 ttactgtatc tactttaagg aggatgagat gacctttacc gtagatagtt acttagaata
     2521 ctttcttacc ttactggctt ggatcatcaa caacaatatt ttcgctgtat tggttcaaac
     2581 agggttgttt gttctgcctc tcatttttgt cttaatcagt acgtggatgg aagtcaaaaa
     2641 acaaggtgag gacgaaggta ataaagggga tttacttatt acttggctca gcctcaaatt
     2701 ttacccggcg atgtttgtcg ttgtattggt cttagctcca atgataccga tcaacctgaa
     2761 taatattgag ttgaatgtag aacgttctaa agcctgtggt tatcgtgttc caactgc
//
LOCUS       DANLXQ010000222         2803 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.226, whole genome shotgun sequence.
ACCESSION   DANLXQ010000222 DANLXQ010000000
VERSION     DANLXQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.226"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            416..709
                     /locus_tag="PXD94_002529"
     CDS             416..709
                     /locus_tag="PXD94_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HDL6287333.1"
                     /translation="MFKVIFMALSAIVNYLISDFLIKGVIFLSVYFLTSELLPVVIEM
                     LVSRKLIPDLNALFGQLPESMWYFLNIFQIPMAISLMISAMISRFVRRMPFIG"
     gene            719..1258
                     /locus_tag="PXD94_002530"
     CDS             719..1258
                     /locus_tag="PXD94_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287334.1"
                     /translation="MAVSAYIGIPGSGKSYEVVKSVILPAVASNRRVVSNIYGLNKEK
                     IHQYLLRKNKKLMIEELGEVIYVTNEQCLESDFLPSMDEQDSFCKAGDLIIIDEVWRI
                     WGNDKDIPKNHRSFIAEHRHFADPITGVTCDLVVINQDISQIPRFIKEGLRQLTECKN
                     TLVWGCVIVIGLMFFKERI"
     gene            1189..1752
                     /locus_tag="PXD94_002531"
     CDS             1189..1752
                     /locus_tag="PXD94_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287335.1"
                     /translation="MQKHVGLGLRNRYRVDVFQGKNLAKKNRTNSYQEKYDKKIFELY
                     KSYESDGAREAVTDSRQSIFSSFWVKVWIILLPLALFYCGYNLYIFLNGSESEVSHNT
                     EQAKFASDENHNDIVIHLLPTIGKPLSTKWRITGELKRDNKSFVILADNENRLRLEPS
                     SQFSFSGRMLQGEIDGGIVSYFSGESK"
     gene            1749..>2803
                     /locus_tag="PXD94_002532"
     CDS             1749..>2803
                     /locus_tag="PXD94_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HDL6287336.1"
                     /translation="MKIHQLGLLLMISSFSYAKNVDFKLEAVPLPKAISIIYDEVLQK
                     PYMLDPKLSNDSRVISFHTTEQQNFEQFIQRYFSNMNISVSEKKGVVYIGHIEPKQPK
                     VIKRSFVYSPIHRDVDYLASFLKDGTEGNQVFASGDKLVYYGTSEDIARVKSILKSID
                     TKSREVVVTGYVFEVQNIEKEGSGINLLAKLLSGKLGISVGYKQSYENFITVNAGNLD
                     AMFELFRTDQRFRVVSSPTLRVKSGSRGNFSVGSDVPVLSNVTYQDGRPIQSIEYRSS
                     GVIFDIQPTIKDKAIDLKIQQLSNFIKTDTGVNNSPTLVKRDLVTDVTVKSGDVIVLG
                     GLAENKISNGETSFSLLP"
BASE COUNT          920 a          390 c          561 g          932 t
ORIGIN      
        1 gtcttgtgtt taatgagagg gttgttagaa taaaacatga gagtttcctt tttgtttaga
       61 ttgatttttg acactcatat tctaaacggg aaactctcac tttttagagt gaattgtcag
      121 atcaagtctg atcttctaca cctgatatta atttgcctga tctggaagtt cctacggcgg
      181 aatcaattat atcccctttt aaaaaattgc taccagattt acagaatttt gaactgaata
      241 aacgaaccgc acaatgccct gtttggacat ttaatattga atacttgaga tttcattatg
      301 aacttagaga acattgcgat ttgatagaac cacataaaga aatcttaaag ctattattcg
      361 gcattatttg ggcgtttatt gctttacgtt atgtattaag tgcttaggag gataaatgtt
      421 taaagtgatt tttatggcac tatcagcaat tgtgaattat ttgatttcag attttttaat
      481 taagggtgtc atatttttat ctgtctattt tttaacttct gaactacttc ctgtagtaat
      541 agagatgctt gtgtctagaa aattgatacc tgatttaaat gcgttatttg ggcaattgcc
      601 tgaaagtatg tggtattttt taaatatttt tcagatacca atggctattt ctttaatgat
      661 ttcagcaatg atttcacgtt ttgttagacg tatgccattt attggataga ggagtgctat
      721 ggctgtttca gcgtatatag ggattcctgg aagcggcaaa tcgtatgaag tggttaagtc
      781 agtaatttta cctgctgttg cttcgaatcg aagagttgtt tccaacattt atggattaaa
      841 taaagagaaa attcatcaat atttactgcg taaaaataaa aaattaatga ttgaagagct
      901 tggtgaagtc atatatgtaa cgaatgaaca atgcttggaa tcggattttt taccatcaat
      961 ggacgaacaa gattcatttt gcaaagctgg ggacttgata attattgatg aagtttggcg
     1021 tatttgggga aacgacaagg atattccaaa aaatcatcgt tcatttattg ctgaacatcg
     1081 acattttgca gatcctataa caggtgtaac ttgtgattta gtcgtaataa atcaagatat
     1141 ttctcaaatt ccaagattta ttaaagaagg attgagacaa cttaccgaat gcaaaaacac
     1201 gttggtttgg ggttgcgtaa tcgttatcgg gttgatgttt ttcaaggaaa gaatttagct
     1261 aaaaagaacc ggacaaattc ttatcaagaa aaatatgata aaaaaatatt tgagctttat
     1321 aaatcttatg aatctgatgg tgcaagagaa gcggttacag atagtaggca atctatattt
     1381 tcatcttttt gggtcaaggt atggattatt cttttaccat tggcattatt ttattgtggc
     1441 tataaccttt acattttcct aaatggttca gagagtgaag taagccataa tactgaacag
     1501 gctaaatttg cttctgatga aaaccataac gatatagtaa ttcatttact tcctactatt
     1561 ggaaaacctt tatcaactaa atggcgaata acaggggaac taaaaaggga taataaatca
     1621 tttgtgatct tagctgacaa tgaaaaccgt ttaagacttg agcctagtag ccagtttagt
     1681 tttagcggtc gaatgttgca aggagagata gatggtggaa tagttagtta tttttcggga
     1741 gaatctaaat gaaaatccat caattagggc tattgctcat gattagctca ttttcttatg
     1801 ctaagaatgt tgattttaaa ttagaagctg tgccgttacc gaaagcaata tcaataatat
     1861 acgatgaagt gctacaaaag ccatatatgc ttgatcctaa attatctaat gattcaaggg
     1921 ttattagctt tcatacaaca gagcaacaga attttgagca gtttattcaa cggtatttca
     1981 gcaatatgaa tatcagtgta tcagagaaaa aaggtgttgt ttatattgga catattgaac
     2041 caaaacagcc gaaagtaata aaacgtagtt ttgtgtattc gcctattcat cgtgatgttg
     2101 attatttggc tagcttcctg aaagatggaa cagaaggaaa ccaagttttt gcaagtggcg
     2161 acaagcttgt atattatggt acttctgaag atattgcacg agtgaaaagt atcttgaaat
     2221 caatcgacac aaaaagccgt gaagtagtgg taactggtta tgtttttgaa gtgcagaata
     2281 ttgaaaaaga gggtagtggc ataaacttgt tagcgaaatt gctatcagga aaattaggca
     2341 ttagtgttgg ttacaagcaa agttatgaaa actttatcac cgtgaatgca ggaaatttag
     2401 atgcaatgtt tgaattgttt cgcacagatc agcgttttag ggttgtcagc agtccgacat
     2461 taagagtaaa atcaggctca agaggtaatt tttcagtagg ctctgatgtc cctgtattat
     2521 cgaatgtaac ctatcaggac ggaaggccta ttcagtcgat tgaataccgt tcaagcggtg
     2581 taatatttga tattcagcca acgataaaag ataaagcgat tgatttaaaa attcaacaac
     2641 tatcaaattt catcaaaaca gataccggtg taaataatag cccaacgttg gtaaagcgtg
     2701 atctggtaac agatgttacg gttaaaagcg gtgatgttat cgttttaggt gggttagctg
     2761 aaaataaaat atctaatggc gaaaccagtt tttcattgct acc
//
LOCUS       DANLXQ010000223         2787 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.227, whole genome shotgun sequence.
ACCESSION   DANLXQ010000223 DANLXQ010000000
VERSION     DANLXQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.227"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            21..>2787
                     /locus_tag="PXD94_002533"
     CDS             21..>2787
                     /locus_tag="PXD94_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S6"
                     /protein_id="HDL6287337.1"
                     /translation="MKTKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENK
                     GPFSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGY
                     KNVQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNA
                     GTSIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPD
                     HWLDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNR
                     SAIIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSS
                     IANQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGA
                     GIIVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQ
                     GRKQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKH
                     ARIVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLK
                     GNSRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNG
                     ILNVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNK
                     HDVVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTP
                     SCYDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQAD
                     QTTHITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKG
                     NGRINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGN
                     KASISLEN"
BASE COUNT          978 a          533 c          513 g          763 t
ORIGIN      
        1 taaggataaa cttctcattt atgaagacca aaacatttac tcgttcttat cttgcttctt
       61 ttgtaacaat cgtattaagt ttacctgctg tagcatctgt tgtacgtaat gatgtggact
      121 atcaatactt ccgcgatttt gccgaaaata aaggaccatt ttcagttggt tcaatgaata
      181 ttgatattaa agacaacaat ggacaacttg taggcacgat gcttcataat ttaccaatgg
      241 ttgattttag tgctatggta agaggtggat attctacttt aattgcacca caatatttag
      301 ttagtgttgc acataatact ggatataaaa atgttcaatt tggtgctgca ggttataacc
      361 ctgattcaca tcactatact tataaaattg ttgaccgcaa tgattatgaa aaggttcaag
      421 gagggttgca cccagactat catactcctc gattaaataa attagtaaca gaagttgtgc
      481 ctgccgcagt caccaatgca ggtacatcta ttaaacccta cttaaatgaa gaacgcttcc
      541 ctatgtttct tcgtgctggt tcagggacac aagcgctaag aggaaaagaa agtaataaaa
      601 caactggaat cgctggtgct tatgaatatc ttactggcgg taccacatta caattatcta
      661 aaagctcccc tgatcactgg ttagattatt caagtaacct ttatcaagta agctatggac
      721 cactttcaac ctatgcacta cctggtgata gtggttcagg ttcttacgcc tatgatatga
      781 acgaaaaacg atgggtatta gttggtgtgc tcaatttcta taatggtatg gataatcaat
      841 tcaaccgctc tgcgattatc cgtaaagatt tccacgagaa aaaatttgcc gaagatattg
      901 caggaacaat caataatacc gtacaaaatg cacaattcaa ttggactgct caaggtaaat
      961 ccagctctct tagtcaatca aataatgtgc aaaaactcaa cgttgatcta aaagatagta
     1021 gcattgcaaa ccaaaacact tctctgccac aagaaaatca cggtaaaacc attaatttta
     1081 atggtaaaga tgcaactatt gtactaaaac aggatattga ccaaggtgca ggtgcattaa
     1141 atctgaacgc taatctcact attcgtcctg aaacagacca aacttggcaa ggtgcaggta
     1201 ttatcgtcgg taaagataaa aaagtgaatt ggcaagtaaa aaatccacaa ggcgatcgtt
     1261 tatctaaact cggggaagga acactctatg taaatggacg tggacagaat cttggcgata
     1321 tcagtgtagg tgatggtatt gtaatactta accagcaagc cgatcaccaa ggaagaaaac
     1381 aggcctttaa tacagtagga atcgtaagtg gtcgccccac tgttgtgcta ggtagtgcag
     1441 atcaagttaa tcccgataat atttactttg gatttcgcgg aggtcgttta gacctaaacg
     1501 gtaacagcat cgcctttaaa cgtattcaaa acagcgataa acatgctcgt attgtaaacc
     1561 acaatcgcga tcacatttct accttaataa tacaaggcca agatcctctc actagtaatg
     1621 atcttatatg gggaaaatgg gcaagtaata gcccagcaga catttacgaa tataccaatc
     1681 cttatcaaaa taaacgcaaa gattacttcc gtctgaaagg taattcgaga gtatattatc
     1741 caacgaatgc tacaagtaac gatcactggg aatttctttc cagtaaccgc gagcaagcaa
     1801 tacagaaaat cctagatgcc aaaaacttaa gacagcgcta tgacacgttt aatggtttta
     1861 taggggaaga tgcttccaat aaaactaatg ggatattaaa tgtcgtgttt gatacaaaaa
     1921 cagaagtaaa tacagaacaa gataaattaa agaatatcta cacaatgtcg ggaggattta
     1981 accttaatgg tgaactcacc cttaaaggtg gtacattgtt gctttctggt cacccaacgc
     2041 cacacgctta tgatattaag aataagcatg atgttgtgcg tgaaaacgat tggcaagaca
     2101 gccattttac tgctaaaaat atcacggtaa ataaaatggc acaactctat atcgggagaa
     2161 atgtcaatga agtaaatagt cactttactg cgactgataa agccaaactc aatttaggat
     2221 ttattaatcg ttcaacgcca agttgctatg attctgaata cacaggcact acacattgtg
     2281 aagtgcaagc ggtcatttcc gataatattt ttgcaaatct agcaacaacc gccattaaag
     2341 gtaatgttaa attacaaaac catagccaat taaatttagg caaagcaaac ctcactggtt
     2401 ctgtacaagc tgatcaaaca actcatatca ctttagcaaa tcacagtcac tggttaaaca
     2461 atggtacgag ccagattggg catcttacaa tggaaaaagg gtcgatcctt agcctaaacg
     2521 ataaatttgc taccacggaa atcccagtcc gattcaacaa gatgatcatc caaggtaatc
     2581 taaaaggtaa tggacgaatt aactataccg caaatttagc caagggcgaa tctgatcatc
     2641 tccaagttga cggtattgct gaaggaaatt ttgtccttgc cgttagaaat agcacaactg
     2701 aagcaaatcc aaaaagctca ttaaacctac taagcttaaa aaatagcaac caagaaggca
     2761 ataaagcttc tatttctcta gaaaata
//
LOCUS       DANLXQ010000224         2772 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.228, whole genome shotgun sequence.
ACCESSION   DANLXQ010000224 DANLXQ010000000
VERSION     DANLXQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.228"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            438..2714
                     /gene="gshAB"
                     /locus_tag="PXD94_002534"
     CDS             438..2714
                     /gene="gshAB"
                     /locus_tag="PXD94_002534"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL6287338.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKRKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVVGDGIHSVKELVERKNDDPLRGDGS
                     RSPLKKIALGEIEQLQLKEQGLTVDSVPAKGQTVQLRANSNISTGGDSIDMTDQMHES
                     YRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQGK
                     SRRLTKAVIKMLFPEIGN"
BASE COUNT          838 a          564 c          589 g          781 t
ORIGIN      
        1 tcattaagag tatttataat acttactcca gttgtggctc catctttaat atatagaata
       61 tctccctttt caggattaca acggctgaaa atttcattat gaatttcttt tgttacatag
      121 ctaatttttg ataaatctaa tccatctttc tttatatttt tagctgtaat ataaagaaaa
      181 tcactcttat cgatatttgg gggagaatga tgagttccat ctgtaatttt taggcatata
      241 tcccccaacc tcacccacac ccaactctca ggaatctcaa aaggtggttc aatggtataa
      301 ggggctttat ccgaagcagt atttctgtta taaccaaatc atctacttca ttccaataaa
      361 ctaaaaccca ctatttcact aagtctttca tttgtgttat tgtgccgata catttttttg
      421 actacaaagg atagatgatg aaactacaaa aacttatccg agaaaatcat ttagcattgc
      481 tgtttcaaca agggaatttt gggcttgaga aagagagcca acgggttgat cgcaacggca
      541 atatcgttac tactccccac cctgccgtat tcggtaatcg ctcttaccac ccttatattc
      601 aaaccgattt tgccgaaagt cagctagagt taatcactcc cccgaatgct aaattagaag
      661 acagttttcg ttggctctct gctattcacg aagtggtatt gcgttcgttg ccagaggagg
      721 aatatatctt ccctctcagt atgccggcag gcttgccgcc ggaagagcaa attcaagaag
      781 cacaatttga caaccctgaa gatgtgcaat atcgccaaca cctttccaaa aactatggga
      841 aatataagca aatggtaagc ggcgtgcatt ataattttca actttcaacg gaatttgtcg
      901 aaaaagcctt tgctttacaa acggaatata gcgagctgaa agccttccaa aacgcacttt
      961 atatgaagtt agctaataac tttttgcgtt atcaatggat tttgctctat ttattggctg
     1021 ccacgcctac ggtggaatcg caatattttt atgaaaaatc accgcttgcg agagggcaac
     1081 ttgtccgcag cttgcgttca agcccttatg gttatgtgaa ttcggaaaaa gtggtggtta
     1141 atcacgacag cttagaaagc tatgttgaaa gccttgaagc caatgttgcc aacggattat
     1201 taattgccga aaaagaattc tattccaatg tgcgtttacg agggggcaaa aaagcccgtg
     1261 agctattgga aaacggtgtg aaatacgccg aattccgttt atttgattta aaccctttcg
     1321 ccccttacgg cattgagctg aatgatgcca aatttatcca ttatttctta ctcggtatgc
     1381 tttggctgga ggaaacaagc ggtcaaaaag aggtcgaaat cggcaaacgg aaactttacc
     1441 aagtcgcact tgaagatcca agaacagaaa ccgctttccg tgccgaaggt gaagccattt
     1501 taaatcaact tttagcgatg cttgaagaat tatctgccgg tcaggaaagc ctagatttgg
     1561 tgcgggaaaa attagctcaa tttgctgatc caagcaaaac cctgaacgga cgtttaattg
     1621 ccgcaataga ggaagtcggc agttataaag cattgggggc taaattggct caaacctata
     1681 aggcacaagc ctttgagcgt ttttatgcgt tatcggcatt tgataatatg gaactttcca
     1741 ctcaagccct gttttttgat ttgatccaac aagggattga ggtggaagta ctggacgaaa
     1801 acgatcaatt cctcgcccta aaatttggcg atcatttgga atatgtgaaa aacggcaata
     1861 tgaccagccg agatcaatac atttcaccgc tgattatgga aaacaaagtg gtgacgaaaa
     1921 aagtattggc aaaagccggc tttaacgtgc cgaaaagcgt tgaatttacc aaccttgaac
     1981 aggctgtggc acattatccg ctatttgaag gcaaagcggt ggtgattaag ccgaaatcga
     2041 ctaactatgg tttagggatt accatcttcc aacaagcggt caaaaatcga gaagattttg
     2101 caaaagcggt tgaaattgcg ttccgtgaag acaaagaggt gatggtggaa gactatttag
     2161 tcggtactga ataccgcttc tttgtactgg gcgatgaaac ccttgcggtg ttactgcgtg
     2221 taccggcgaa tgtagtggga gatggcattc acagtgtaaa agagctggtg gagcgtaaaa
     2281 atgacgatcc gctacgtggc gatggctctc gtagcccgtt gaaaaaaatt gccttaggtg
     2341 aaattgagca gttacaactt aaagagcaag gcttaaccgt agattccgta ccggcgaaag
     2401 gtcaaacagt tcaactgcgt gccaattcca acatcagcac cggcggcgat tcgattgata
     2461 tgaccgacca aatgcacgaa agctatagac aaattgcagt gggaattgct caggcaatgg
     2521 gggctaaagt gtgtggggtg gatttgatca ttcctgatct caccaaacct gccgagccaa
     2581 atttaagctc ttggggcgtc attgaggcaa acttcaaccc aatgatgatg atgcacattt
     2641 tcccttacca aggcaaatcc cgccgtttaa ccaaagcggt aatcaaaatg ctgtttcctg
     2701 agataggcaa ttaaattttc tctctatacc tcttttaagg agaatttact caagaattct
     2761 ccttaattta tt
//
LOCUS       DANLXQ010000225         2715 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.229, whole genome shotgun sequence.
ACCESSION   DANLXQ010000225 DANLXQ010000000
VERSION     DANLXQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.229"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            50..1168
                     /locus_tag="PXD94_002535"
     CDS             50..1168
                     /locus_tag="PXD94_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL6287339.1"
                     /translation="MKKTLVALAVTAFAASASATTVYDAEGSKVDFDGSLRIVLDRTN
                     SKELDNHNVVKTKKDAHTGIRNTGSRFGVRVKHDLAQDFYALGRVEFRFNKNTSDDGF
                     GDLYTKRAYVGLGSKQYGELTFGRQVTIADDLSQSVDYNYGINPKGDYIQTDGNQVIR
                     YNYRGVENLHVSANYNFAQDRKDGKVLANKLQNAYGIGALYTVDNWDLRAAYGHTNYK
                     TATDSTKHRKDGVLGSVGYKFGDLKLSVDTGYSREKGLAVTDDNGVTTFYNGVKSNAF
                     YVSPNFEYQVVPASKVYGNYLYERVKSKSNGSTYAAGKEKTHGFLLGVDYQFHKQVVA
                     FVEGSYTRTKDYDKVTNGYEYSGKSTNKAIGVGMRVYW"
     gene            complement(1321..2685)
                     /gene="glmU"
                     /locus_tag="PXD94_002536"
     CDS             complement(1321..2685)
                     /gene="glmU"
                     /locus_tag="PXD94_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL6287340.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKARLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVTIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
BASE COUNT          782 a          505 c          536 g          892 t
ORIGIN      
        1 atgagggtaa ttacttatca tataacatat aaaatctaag gtgaaaatta tgaaaaaaac
       61 attagttgca ttagcagtaa ccgcgttcgc agcgtcagca tcagcaacca ccgtttacga
      121 tgcagaaggt tcaaaagtag attttgatgg ttctttacgt attgttcttg atagaacaaa
      181 ttctaaagag ctagacaatc ataatgttgt taaaactaaa aaagatgcac acacaggaat
      241 ccgtaacact ggttcacgtt ttggtgtaag agtaaaacat gatttagctc aagatttcta
      301 tgcattaggt cgtgttgagt tccgttttaa caaaaatacc agtgatgatg gttttggtga
      361 tttatatact aaacgtgctt atgttggttt aggtagtaag caatatggtg aattaacttt
      421 tggtcgtcaa gtaacgattg ctgatgattt aagtcaatca gtagattaca actacggtat
      481 taatccaaaa ggtgattata ttcaaaccga tggtaaccaa gttattcgtt ataactaccg
      541 tggtgttgaa aacttacatg taagtgcaaa ctataacttt gcacaagatc gtaaagatgg
      601 taaagtatta gcaaataaac ttcaaaatgc atacggcatt ggtgcattat acactgtaga
      661 taactgggat ttacgtgcag cttatggtca tacaaactac aaaacagcta ctgatagcac
      721 taagcaccgt aaagatggtg tattaggttc tgttggttat aaatttggtg acttaaaatt
      781 aagtgttgat acaggttatt ctcgtgagaa gggtttagct gttacggatg ataatggtgt
      841 aactacattc tataatggtg ttaaatcaaa cgctttctat gtatctccaa actttgaata
      901 tcaagtagtg cctgcatcta aagtatatgg taactactta tatgagcgtg tgaaatctaa
      961 atcaaatggt tctacatatg ctgctggtaa agaaaaaact catggtttct tacttggtgt
     1021 tgattaccaa ttccataaac aagtagttgc ttttgttgag ggttcttata ctcgtactaa
     1081 agactatgat aaagtaacta atggttatga atactcaggc aaatcaacta acaaagctat
     1141 cggtgttggt atgcgtgtat actggtaatc aattgatttt ctaaattgat aaacccctaa
     1201 caatatttat tgttaggggt tttgttttaa atatattgtt cattatcctt tttaaagtgt
     1261 atggctttgc tgttattggc aaagccaatt tttaaatata ataacttttg accactggca
     1321 ctatttcttt ttagtcgggc gttgccagcc ttgaatatgg cgttgtggta cacggctaat
     1381 taccagttca ttttccgcaa tgtctttagt gactgttgca ccggcaccaa tggttgaacc
     1441 gctggcaatc gtgaccgggg caatgagttg gctgtctgag ccgacaaata cgttatcgcc
     1501 aataatggtt ttgaatttat tcgcaccatc atagttacaa gtgattacac ctgctccgat
     1561 gttacattct ttgccgattt ccgcatcgcc tacataagtg agatgattta cttttgaacc
     1621 tttgccgatg gtagcttttt tgatttccac aaagttaccg atatgggttt cttccgctaa
     1681 ttctgccccc gggcgtaagc gagagaatgg accgatagcg gattttgccc cgacaatcga
     1741 atcttcaatc acagaataag gtttaatttc aacattatcg ccaatttcgc agtttttcag
     1801 tacgcagcct gcaccaattt ttacattgtt accgagtttg atttcacctt ctagaatgac
     1861 attcacatcg atctgaacat cttttccgtg ggtgacagtg ccacgaagat caaaacggct
     1921 tgggtcgata atagttacac cagccaataa cagtttttcc gcctcacgtt tttggtagaa
     1981 ccgttcaagt gcagcaagtt gcaaacggtt gtttgcccct tctacttcca taaattcagt
     2041 tgcctgaacc gcttgtactt taaagccttc ttggtttgcc attgcgatca catcagtgat
     2101 gtaatattca ccttgagcat tgttgttatt taggtttttc aaccattttt tgaagctcgc
     2161 accgcttgct accatcacgc cggtattcac ttctttgact ttaagctgtg cttcattggc
     2221 atctttttgc tcaacaatcg ctaccacaga accattttca cgaataattc gtccgtagcc
     2281 tgtcgggttt tccaattcaa cggttagtaa tgcaatacca ttttccggtt tggctgcgat
     2341 taaacgctct aaagtggctt ttgtgattag gggggcatca ccataaagca ttacaatgtt
     2401 ttcatcatca gcgaaaaaag gagctgcttg ttgcattgca tgtcctgtgc ctaattgttc
     2461 ggcttggaat acccagttta caggctcttc gcttaaacgt gcttttaaca gatcggcacc
     2521 gtgtccgtaa attaaatgaa tttgctgggc atctagctgt ttggcagtat caataacgtg
     2581 ttttaccatg ggtttaccgg caactgggtg taacactttg ggtaaatctg aatacatacg
     2641 agtgccttta ccggcagcta aaataacaac gctcagttga gtcatataga atccttattt
     2701 gactaaatag atgaa
//
LOCUS       DANLXQ010000226         2689 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.230, whole genome shotgun sequence.
ACCESSION   DANLXQ010000226 DANLXQ010000000
VERSION     DANLXQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.230"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            139..579
                     /locus_tag="PXD94_002537"
     CDS             139..579
                     /locus_tag="PXD94_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL6287341.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIASGAEILLPILLTLGLFTRFSALGLMILTAVAWYAVHAGNGYNVC
                     NNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQKKSPTKRWLKFL"
     gene            605..892
                     /locus_tag="PXD94_002538"
     CDS             605..892
                     /locus_tag="PXD94_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287342.1"
                     /translation="MKKATLMALAGALTMATATVNAETKSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            993..1916
                     /locus_tag="PXD94_002539"
     CDS             993..1916
                     /locus_tag="PXD94_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL6287343.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            1906..2646
                     /locus_tag="PXD94_002540"
     CDS             1906..2646
                     /locus_tag="PXD94_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL6287344.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDTEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
BASE COUNT          803 a          532 c          578 g          776 t
ORIGIN      
        1 ctataaaaat aggttataat ttttcacaca ttttttgtaa taaacgtata cttattctgt
       61 ctaataatca catttttagt taggagcatt tttatgtcat cattttcgtc atcattttca
      121 actcagtcag cttgctcaat gacagcttgt aaaaacggct tgggctttct tgtattacgt
      181 ctatttcttg cttatgaatt ttttgaagca gggttatcaa aactaaacgg tgaaaattgg
      241 tttgcctcaa ttcaagagag cttcccattc ccctttaacg tactaccagc cgatcttagc
      301 tgggctattg ctagcggagc ggaaatctta ctgcctattt tactcacttt gggtctgttc
      361 acccgcttca gtgcgttagg attaatgatc ttaacagcgg tagcgtggta tgctgttcac
      421 gctggcaatg gctacaatgt ctgcaacaat ggctataaaa tggcactcat ttatttagtg
      481 atgttagtac cacttttatt acaaggtgct ggctcgctct ctctcgattt tctgttacag
      541 aaaaaatccc caaccaaacg ctggttgaaa ttcctttaat cccttaattt atggagaatt
      601 tttaatgaaa aaagcaactt taatggcatt agctggtgca ttaactatgg caaccgcgac
      661 tgtaaacgct gaaactaaat cagtgaaagc agatgcttgt atggaagtaa aagatggcaa
      721 atgtgtaaaa accaaagcta cagaaggtaa atgtggcgaa ggcaaatgtg gagcaacctc
      781 ttctgagcca aaagcaaccg aaggtaaatg cggcgaaggg aaatgtggag caacggcacc
      841 aaaagcggca gaaggtaaat gcggtgaagg caagtgtgga gcttctaagt aagtagcgaa
      901 ggtgcgtttt ctaaacgcac ctaacctctt ataaacacaa tctacaagcg gttaaattcc
      961 gctgattttt tgcaaattaa aggaacaaga cgatgttaaa atttactggt gccggtctag
     1021 gctatcgccg tgacctagcg gacggatttt tatctctcac agataacaat ataattaatt
     1081 ttatggaagt agccccagaa aactggctga aaatgggcgg tcaggctcgg tataacttcg
     1141 ataaagtcgc tgaaaaaatc cctgttatta ttcacggttt atccctctct ctcggcgggc
     1201 aagcgccttt agatatgacg ctactaaaag ggatcaagaa tatgcagcag cagtataact
     1261 cctttttctt ctctgatcat ttaagctatt gcgaatgtga agggcattta tatgacctgc
     1321 tgccaatgcc gtttaccgat gaagccgtac atcacgtggc aaatcgcatt aaacaagtgc
     1381 aagattattt aggtatgcaa atttcccttg aaaatacgtc ctactatctg cattcaccaa
     1441 ccagtacgat gaatgaggta gagtttctca atgcaattgc ccaagaagcg gattgtggca
     1501 ttcaccttga tgtgaataat atttatgtta atggtgtcaa tcacggcttg cttgatccat
     1561 ttgtatttat tgatcaagtg gataaatctc gtgtcaatta tattcatatt gcaggccacg
     1621 atgaagatca cggtgcggcg gaagacgtta aaatgaccga gggtgaatca tttaatatgg
     1681 taaaaggcga actacgtcat ctgccaactt tactggtcga tacacacgga gaagctgtac
     1741 aaaaaactgt gtgggattta ctagagtaca cctatgaaca cctaccgcat attccaccaa
     1801 cactgttaga acgggatttc aacttcccac cgtttcaaga actgattagt gaagtacagc
     1861 acattatgag cttacagaaa aaatatgcaa aaacgaggct aaaaaatgcc gcttaaactc
     1921 gttgaagaac acaaaccgtt actcaaagag attcaagccg agtttgcgaa tgcaattcgc
     1981 cataacgatg taaagctgat gagtggtaat atctctccac agcggcttgg cgtctattct
     2041 cgcttggttc gcaataatac tctcggtttt atcgaccgtt gttatgtaca gctccctcaa
     2101 catctaagtc ctgaagaatg ggttgccgtt aaagaaaaat ttatacgaga aggtaaggct
     2161 cattcgccct ttttccaaga tattgctggc gaatttctca atttttgccg agaaaatggc
     2221 tgtatgccac cacacttgct tgagttgatg gactttgaaa atgctcaatt actggctgag
     2281 gtatcaatgg caaccgtacc atcagaattt gaatgggata acgatactat aatgcagttt
     2341 tctggtgcag ctgagctgcg tcaatatgcg gtaaacttta tccgtagcga gttcaaacag
     2401 tttgactttg agccgaccaa cgtactgatt tggcgagata cggaatttgg catctattac
     2461 aaccgcttgg aagagctgga tttcttcctg cttagctcgt tgcaagaggg ggcaaattcc
     2521 ctgaatggct tactagcaga actcagcgag tttaccgagc aaaatctgga gctaaaagcc
     2581 ttgttacgcc aactttggga aaaatgggtt gaagcagagg tgatttatcc tgtctatcct
     2641 gaatgaggat aaggtatgtg atatgtactg acatacatag taggggcgg
//
LOCUS       DANLXQ010000227         2670 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.231, whole genome shotgun sequence.
ACCESSION   DANLXQ010000227 DANLXQ010000000
VERSION     DANLXQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.231"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            52..810
                     /locus_tag="PXD94_002541"
     CDS             52..810
                     /locus_tag="PXD94_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL6287345.1"
                     /translation="MIPLERQREILRLINNNGVASINFLAEQLKVSHMTIRRDIQKLE
                     EQGKVSLVSGGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGT
                     TTLEIAHRIIDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEF
                     LKNLSIDMAFISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFF
                     VCPLTVFDQIICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(950..2341)
                     /gene="ffh"
                     /locus_tag="PXD94_002542"
     CDS             complement(950..2341)
                     /gene="ffh"
                     /locus_tag="PXD94_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL6287346.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAEALKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT          808 a          557 c          516 g          789 t
ORIGIN      
        1 tggaaactaa atctgatata atctctttta acaattaaaa taggttaaaa aatgatcccc
       61 ttagagagac aacgagaaat actacgactt attaataata atggtgtcgc aagtattaat
      121 tttctggcag aacagctaaa agtatcacat atgacaattc gccgtgatat tcaaaagctg
      181 gaagagcaag gtaaggtatc cctagtatct ggtggagttc aatctcttga acgattaatc
      241 atggaattac cacacgatga taaatcaatg ttattccaag agcagaaatc agctatagga
      301 tctttagcat cacagctaat tccctcaaac acaactattt atcttgatgc tggaactact
      361 acattagaaa tagctcatcg gataatcgat agaaatgact tactagttgt aacaaacgat
      421 tttgttattg ctcactatct tagtaattca ggaaaatgtg atttaattca tataggagga
      481 agtgtctgta aagaaaacct atcgacaact ggttcacttg cagcagaatt tctgaaaaac
      541 ctatcaatcg atatggcatt tatatctact tcatcctgga atttaaaagg tctaacaacg
      601 ccgaacgaaa ataaaatccc cgttaaaaaa gcaattttag aggcatccaa gcaaaacatt
      661 ttagtgacag actcttcaaa atatggaaaa gtcgcaactt tttttgtttg ccctcttact
      721 gtatttgacc agattatttg tgataaagga ttatttgaga atgtacgaga cgcactgaaa
      781 gggctaagta ttaaattaga tttaggataa tcttcttacc aattttttct gagaacgaca
      841 agcaatcaaa aagtattatt tttgcaaata agatattaat agggaaagtt aacaatccta
      901 gcaacggatt gttaactgat tttcggctgg tactaataaa ataggaatat taacgtttac
      961 caaacatact gcctaagcca ccgagaccgc ctagaccacc acccatcata cctttaacac
     1021 cacgcatcat ttttgccatt ccgcctttac gcattttttt catcatacgt tgcatatcat
     1081 caaactgctt gagcagctta tttacatctt gcacctgtgt gccggagcca agtgcgatac
     1141 ggcggcggcg tgagccttta ataatatccg gatttgcccg ctcttttaag gtcatagaat
     1201 tgatgatggc ttccattttc atgaacattt tatcatcaac ctgactttta acgtggtcag
     1261 gcaggttttt cgcccccggt agtttatcga gcatcgacat cattccaccc atttttttca
     1321 tttcgataag ctgctcacgg aaatcttcca aggtaaattc atcgcctttt ttgaatttgg
     1381 cggcaatttt ttccgctttt tcttgatcaa ccgagcgttg aatatcttca attaaagaca
     1441 ttacatcgcc catacctaaa atgcgggaag ccacacggtc agggtggaac ggctcaaggg
     1501 catcagtttt ttcacccaca cctaagaatt taatcggttt accggtgatt tggcgaatag
     1561 aaagtgccgc accgcctcgg gcatcgccat cgactttggt aagaataaca ccggtaagcg
     1621 gcaacgcttc gttaaaggct tttgcggtgt tggcggcgtc ttgaccggtc atcgcatcaa
     1681 ccgtaaatag cgtttcaatc ggattcagca cagcgtggat ttgttggatc tcttccatca
     1741 tttcgccatc aacgtgtaaa cgaccggcag tatccacaat caatacatca aagaaattaa
     1801 gcttagcgtg cttcagtgcc gcttctgcaa tcgctaccgg tttttggctg gtttcggtcg
     1861 ggaagaagtc cacttttaac gcttcagcaa gggtctgtag ctgcttgata gcggcagggc
     1921 ggtaaacatc ggcagaaact accaacacct ttttcttgtg gcgttctttc aggaattttg
     1981 ccaatttgcc gacagaggtg gttttacccg caccttgcaa acctgccatt aaaattaccg
     2041 caggcggttg ggccgcaaga ttcagctcct cgttggcttc tcccattgcc gtttctaatt
     2101 cgctttgtac gatttttaag aactcttgcc ccggcgtgag gcttttgttt acctcaaccc
     2161 ctaacgcacg ctctttgacc ttgttgatga aatcacgcac aacagggagg gcaacatccg
     2221 cctccaacaa tgccatacga acttcacgca gggtatcttt aatattatct tccgttaggc
     2281 gacctttgcc cgtaatgtta cgcagcgttt tagacagtct gtcagagagg ttttcaaaca
     2341 tatcagcttt ctcaatttgc aaaattttag gaaaaaagta ccgcttgtga gcggaaagtt
     2401 gccgccatta tacaaaaaaa agcgttacgg ttatagggaa ataaaaaaac ggcatagcga
     2461 ttaaactatg ccgtaaaatt tgacgatatt cgaggaatca ttatttagtt atattgtgcg
     2521 atcataatac tccgaattgc gtataaaagt gaagtgatat tttcaaattt atgttagaaa
     2581 tgagaggtaa attacaaaat taaaggggat attattgttt gaaaaaataa gagcgaaaag
     2641 aaaaccctga tgattgctca tcagggcttc
//
LOCUS       DANLXQ010000228         2665 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.232, whole genome shotgun sequence.
ACCESSION   DANLXQ010000228 DANLXQ010000000
VERSION     DANLXQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.232"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            161..451
                     /locus_tag="PXD94_002543"
     CDS             161..451
                     /locus_tag="PXD94_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL6287347.1"
                     /translation="MALRPLHDKVILKREEVETKSAGGIVLTGSAATKSTRGKVIAVG
                     TGRILDNGSVQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            520..2160
                     /gene="groL"
                     /locus_tag="PXD94_002544"
     CDS             520..2160
                     /gene="groL"
                     /locus_tag="PXD94_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL6287348.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2285..2611)
                     /locus_tag="PXD94_002545"
     CDS             complement(2285..2611)
                     /locus_tag="PXD94_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL6287349.1"
                     /translation="MLKRILGITLCTILVSSNAIASVKTSSLTVAKPTLSKNLPTECE
                     KMFNTANKLVSDAERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNS
                     LKQKHQ"
BASE COUNT          848 a          430 c          628 g          759 t
ORIGIN      
        1 ttcttaggta tatagtttta tcaaaaatgg tcaaatttgt aaaaaatttt acaaatttga
       61 ccgcttgcac ttgaaactct aaaatctgcc tttatattgt taggcgttac gcattttgcg
      121 tgaagtttta tttattcatt tttaagaaaa ggaacatatt atggcacttc gtccattaca
      181 cgataaagtt attttaaaac gtgaagaagt tgaaacaaaa tcggcaggcg gtattgtttt
      241 aaccggttcg gcagcaacaa aatcaacccg tggcaaagta attgcagtag gaacaggtcg
      301 cattttagac aatggttctg ttcagccttt agcagttaaa gtgggcgatg tagtgatttt
      361 taacgaaggc tacggcgtaa aaaccgagaa aattgacggc gaagaagtgc taattttagc
      421 agagcacgat attttggcga ttgtagaata atttcgttct gcacaagcgg ttaaaaaagc
      481 aaaatttttt acatatttca aatacaaagg atttagaaaa tggcagcaaa agacgtgaaa
      541 tttggtaacg atgcacgcgt aaaaatgtta aaaggcgtga atattttagc cgatgcggta
      601 aaagtaactt taggtccgaa aggtcgtaat gtggtgttag atcgtgctta tggtgcacca
      661 acgattacaa aagacggtgt gtcggttgcc cgtgaaatcg aattagaaga taaatttgaa
      721 aatatggggg ctcaaatggt gaaagaggtt gcctctaaag ccaatgatgc cgccggtgac
      781 ggtacaacta ccgcaactgt attagctcaa gcgattgtaa acgagggctt aaaagcagtg
      841 gctgcgggta tgaacccaat ggatttaaaa cgtggtatcg ataaagcggt tgcagcagtg
      901 gttgaagagt taaaagcgat ttcaaaaccg tgcgaaacct caaaagaaat tgagcaagta
      961 gggacgattt ctgcaaactc agattcaaca gtcggtcaat taattgctca agcaatggaa
     1021 aaagtgggca aagagggcgt gattactgtt gaagacggta caggtttaga agatgcatta
     1081 gacgtggttg agggtatgca atttgaccgt ggttacttat cgccatactt tatcaataaa
     1141 ccggaagccg gtacggttga gttagaaaac ccatatattc ttttagtgga taaaaaagtg
     1201 tctaacattc gtgaattatt accggtgtta gaaggtgttg caaaagcagg taaaccgtta
     1261 ttaattattg ccgaagatat tgaaggcgag gcgttagcta ccttagtggt gaacactatg
     1321 cgtggtatcg tgaaagtggc ggcagtgaaa gcacctggct ttggtgatcg ccgtaaagca
     1381 atgttacaag atattgcgat cttaactgca ggtactgtga tttctgaaga gatcggtatg
     1441 gagcttgaaa aagcaaccct tgaagagctt ggtcaagcaa aacgtgtggt tatcagcaaa
     1501 gataacacca caattattga cggtattggt gatgaaactc aaatcaaagg tcgtgtagca
     1561 caaatccgcc aacaaatcga agattcaact tcagattacg acaaagaaaa actacaagag
     1621 cgtgtagcaa aattagccgg tggtgttgcg gtaattaaag taggtgcggc aactgaagtg
     1681 gctatgaaag agaaaaaaga ccgtgttgat gatgctcttc acgcaacccg tgctgcggtg
     1741 gaagaaggta ttgtaccggg gggcggagtt gctttagtgc gtgcagccaa caaagtggct
     1801 gatacactaa aaggcgataa cgaagagcaa aatgtaggta tcaaacttgc cttgcgtgca
     1861 atggaggctc cattacgcca aatcgttgcc aatgcaggtg aagaagcctc agttgtagcc
     1921 cgcaacgtga aagaaggttc aggcaactac ggctataacg caggtacaga gcagtacggc
     1981 gatatgcttg aaatgggaat tttagaccca actaaagtga ctcgttcagc acttcaattc
     2041 gcggcttcta ttgcaggttt aatgatcact acagaatgta tgattaccga tctgccaaaa
     2101 gaagagaaat tagatcctgc cgcaatgggc ggtatggggg gaatgggcgg aatgatgtaa
     2161 ttgcccattg agtgaacaaa agctactgaa attaattcag tagctttttt gttggtaaca
     2221 agtggttaaa tttatgctat tttttgcaaa taaaaaacca agcaagtaag cttggtttta
     2281 gcgtttattg atgtttttgt ttaaggctgt ttagtgctgt taaacctttc tcacaacttt
     2341 tttgctgtaa ttcagaatcc atttttagga tttgttgctt tgttaaagaa agtttatttt
     2401 tcatttttag cacttgagta tgagtaccag gctgtctttc tgcatcagag accaatttat
     2461 ttgccgtgtt aaacattttc tcacattcag ttggcaaatt tttacttaaa gtaggttttg
     2521 caactgtaag tgatgaggtt ttaacacttg ctatagcatt actggaaacc aatatggtac
     2581 aaagtgttat tcctaaaatt ctttttagca tgcgttctcc acacattaag tgtttaaaag
     2641 taaaaatcta atatgttatt taata
//
LOCUS       DANLXQ010000229         2618 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.233, whole genome shotgun sequence.
ACCESSION   DANLXQ010000229 DANLXQ010000000
VERSION     DANLXQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.233"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            98..1390
                     /locus_tag="PXD94_002546"
     CDS             98..1390
                     /locus_tag="PXD94_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6287350.1"
                     /translation="MEKSVPIFNETELRANQLLTEKLLIQAIYSRRLANMEKLTAIYR
                     DFTHIDPVLLRYAQGKIALLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQL
                     NQAQAQFEKAKSAEHLPPKVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQP
                     NIEQTGYVKQPNLLPQTAHGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDE
                     ITNRTLFGYAYKKSDQTTRLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATAL
                     EYGKKRYFDLPIYNGSYKLASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIR
                     FGWQREWQALELSSRLTLGYTDRRYKDTAILGGKLNLGRIRHDRVQSANLTLWKRDWH
                     WLGLMPKLQFSYAKHRSNLPSLYAYTDKNINLIIESRF"
     gene            complement(1640..1963)
                     /locus_tag="PXD94_002547"
     CDS             complement(1640..1963)
                     /locus_tag="PXD94_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL6287351.1"
                     /translation="MQKAYQIYTHSEREKVTEAKLELGKVETRLRNEGKTKEEIAQHP
                     EYLQLKRDLNDKQQTFDNAYGTGSKTKRAVDAVTAALQGLAAKDSGQAAVGLASPYLN
                     EQIKK"
     gene            complement(1932..2459)
                     /locus_tag="PXD94_002548"
     CDS             complement(1932..2459)
                     /locus_tag="PXD94_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL6287352.1"
                     /translation="MRDKNKERQQTLERYQELYDQDPELRVIAANSEGDLDLLQETFF
                     VKGELTITDSTPKEKWNWLHMCNLNSYSPSPLSVVEFYIKHGVDVNAQDCYGMTPLHY
                     AMRAKNVDAAIALLNAGADPNIENKDQVIPLSMIGAVPKEIELIEHDISEVKETQELV
                     KGIGEIAEGLSDLHA"
     gene            complement(2461..>2618)
                     /locus_tag="PXD94_002549"
     CDS             complement(2461..>2618)
                     /locus_tag="PXD94_002549"
                     /inference="COORDINATES: protein motif:HMM:NF025767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287353.1"
                     /translation="QKQFNDYVNARPENFRLENISENRSHKNEMPGKDNIKSIIDDMK
                     KFQQIGK"
BASE COUNT          752 a          618 c          454 g          794 t
ORIGIN      
        1 acttttccca atgatgttat ttgcccgaga tcctaatact acacaggctt atattgagca
       61 tactgcacag ccaactacaa ccccccaaag ggtcaagatc gaaaaatcag tgcctatttt
      121 taacgaaact gaattacgtg ctaatcaatt attgaccgaa aagctactta tccaagctat
      181 ttatagtcgt aggcttgcta acatggaaaa attaactgcg atttatcggg attttaccca
      241 cattgacccg gttttactgc gttatgcaca aggtaaaatt gccctactga aaggcgatta
      301 cgcacaggca attcagcact tccaaaccat tcttgccaaa caacccaaac taaacactgt
      361 aagaattgag ctggcgattg ctctattgta cgatcagcaa cttaatcaag cccaagcaca
      421 atttgaaaaa gccaaaagtg ccgaacatct accgcctaaa gttcacgcat taattgagca
      481 atatcagcaa catctggcaa aaaaacagga atggaaattt agtttttcag ccaattatgt
      541 gcgagacggc aacattaacc gaacctcgaa acagccgaac attgagcaaa ccggctatgt
      601 aaaacaacct aatttgttgc cccaaaccgc acatggtgtt cactatgcgt ttggattaca
      661 gagagatttc aacctgttcg actcacacta tgtgagtttt gacaaccaaa cccaaggtga
      721 actttattgg gataaccacg attatgatga aattaccaat cgcaccttgt ttggatacgc
      781 ttacaaaaaa tccgaccaaa ccacccgctt gttaccgttt tatgaaaaac gctggaaaca
      841 tcaccgttcg gaacgctgga caaacggaat tcgacttgaa cacgcacaat ggttttcacc
      901 gcattggcaa ctcgccaccg cattggaata cggcaaaaaa cgttattttg atttgccgat
      961 ttataacggc agctacaaac ttgcctctgc cacattgctt tggttacgca atccacaaca
     1021 atttttcttt gtcggcactg atttcaatca agacaaaact cagttaaaac aatatagctc
     1081 tgatactaaa ggcatacgct ttggctggca acgagaatgg caagccctcg agctttccag
     1141 tcgcctcacg cttggctata ctgacagacg ttacaaagac accgctatat tgggaggaaa
     1201 acttaatctt ggcagaatcc gccatgaccg tgtgcaaagt gctaacctca cgctttggaa
     1261 acgagattgg cactggctcg gcctgatgcc gaagctgcaa tttagttacg ccaaacaccg
     1321 tagcaacctg ccgagcctct atgcctacac cgataaaaac atcaatttaa ttattgaaag
     1381 tcggttctag ctccataaaa acaacaagcg gttaaaattc ctagaaattt tgcaaattgc
     1441 aaaaaatgta gaaaatttaa ccgcttgtat ctatactgca gcttcttttc cttcagtata
     1501 aattaatata caccgttgca acaataatgg ctgctccctg tcatcacctt gaactgaaaa
     1561 ttgctcaagg tggatatgac tatcgcagcc gcttccaccg cacccagtaa tgtatgggca
     1621 agcaggttgg cttgggtatc tactttttaa tttgttcgtt gagatatggc gaagctaagc
     1681 ctactgctgc ttgtcctgaa tctttcgccg ctaaaccttg taaggctgcc gtcaccgcat
     1741 ccaccgcacg cttggttttg ctgcccgtgc cgtaggcgtt atcaaaagtc tgctgtttat
     1801 cgtttaaatc ccgttttaat tggagatatt caggatgttg agcgatttct tcttttgttt
     1861 ttccctcatt tctcaaacgg gtttccactt tgcccaattc cagcttcgct tccgtcactt
     1921 tttcacgctc actatgcgtg taaatctgat aagccttctg caatctcgcc tatgcccttc
     1981 accagctcct gtgtttcttt cacctcacta atatcgtgtt ctattaactc tatctcttta
     2041 ggaactgctc caatcataga caatggaata acttgatctt tattttcaat attcggatct
     2101 gcccccgcat ttaataatgc tatcgctgca tcaacattct ttgccctcat tgcataatgt
     2161 aaaggggtca ttccgtagca atcttgggca ttaacatcta ccccatgttt aatgtaaaat
     2221 tcgactacag aaagaggaga tggtgaataa ctatttaagt tacacatatg tagccagttc
     2281 catttttctt ttggtgtgct atccgtaata gtcagttcac cttttacgaa aaatgtttct
     2341 tgtaaaagat cgagatcgcc ttctgagttt gctgcaataa ctcttaattc aggatcttga
     2401 tcatataatt cttgataacg ttctaatgtc tgctgacgtt ctttattctt atctctcatt
     2461 ttactttcct atttgttgga attttttcat gtcatcgatt attgatttaa tattatcttt
     2521 ccccggcatc tcattcttat gactccgatt ttcagatata ttttctaatc tgaaattctc
     2581 cggtctcgca ttaacataat cattaaattg tttttgac
//
LOCUS       DANLXQ010000230         2571 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.235, whole genome shotgun sequence.
ACCESSION   DANLXQ010000230 DANLXQ010000000
VERSION     DANLXQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.235"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..395)
                     /locus_tag="PXD94_002550"
     CDS             complement(<1..395)
                     /locus_tag="PXD94_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL6287354.1"
                     /translation="MRSMFKKFFGKNKSQSAVKSDTIFSIEQDNEGWLYPEKASQLLN
                     TELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKVRQSYILPPNVAPEEQSKQR"
     gene            complement(499..963)
                     /locus_tag="PXD94_002551"
     CDS             complement(499..963)
                     /locus_tag="PXD94_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HDL6287355.1"
                     /translation="MMNTQQPKFDLYQHITDRIIAALEQGTAPWLKPWSHPECNMALP
                     RNAVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRP
                     NEREKLDEEGNIVYDEEGNPEMEQHFFIAKHNLFNLDQCENLPAELYDPVIP"
     gene            1172..1435
                     /locus_tag="PXD94_002552"
     CDS             1172..1435
                     /locus_tag="PXD94_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme-like superfamily protein"
                     /protein_id="HDL6287356.1"
                     /translation="MRPVTRNNRGTSFDPRHNKYINTLATALLARWHIEQFSERNIPV
                     NDENLYMAHNIGLDGFHRALLGQSTRKDIENMRKNGMKPWMSI"
     gene            complement(1519..2544)
                     /locus_tag="PXD94_002553"
     CDS             complement(1519..2544)
                     /locus_tag="PXD94_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL6287357.1"
                     /translation="MTAGDTPHDTLKTLVGALKDTREEARKANERGDRFEQEVLRLQA
                     REAEVDSRIAGEVESRVESRLAAAWDGFNRQIEELKGRVQSGNQQQKEAEAIPVGGAS
                     SGFGTESAGQTGNVDNNGIRWVDPDDMVVTDEQGKPVTGNYSGKTKTGFPSPFKAEGE
                     SSNTGTALNNNAGTENMTGNTQIQQIKRTPYYTIPENSTLMGSTAMTALLGRVPIQGA
                     VTDPFPFKVLIGRENLIANGIDLPDVEGAIVSGTSTGDWTLSCVRSEVTSITFVFADG
                     RISNGRASGESRAVKRNQASNLTPFCLTVSLSGFNDIPHSDGNPGFLTMSAVDYVDVY
                     LMSHLAR"
BASE COUNT          706 a          580 c          494 g          791 t
ORIGIN      
        1 tctcgttgct ttgattgttc ttcgggagct acattcgggg gcaaaatata gctttgacga
       61 actttagctg aaaaagcaat aacttctaaa ccgtgatcaa gcattccacc gatatgtgaa
      121 tgatggtgtg attcagaagc tggtaatagt tgaaccatct cagcgtaacg ttctattggc
      181 tgctgataaa gtacctcaaa catttgacgg ttcattgata cctgttgcca aagtagccca
      241 agatattttt gtcgtaattc tgtattaagt aattgagagg ctttttccgg gtataaccac
      301 ccttcattat cttgctcaat agaaaaaata gtatcggatt tgaccgcact ttgagattta
      361 tttttaccaa aaaatttttt gaacattgaa cgcattggaa gagaccgaaa atatgaatgc
      421 tgtattgaat gaaagccgtt tagccctttg cccttttggg taagaccttt ccccttggaa
      481 aggttactct tatccccctt aggggattac aggatcgtac agttccgccg gtaaattttc
      541 acactggtct aaattgaaca gattgtgctt agcaatgaaa aagtgttgtt ccatttccgg
      601 atttccttcc tcgtcataca caatatttcc ctcctcatcg agtttttctc tctcattagg
      661 acggtagttc atcacaacag tagctttttc ccctttacgg atatatccgc cgagttcatt
      721 ggcttttttc gccgtaatcc acttacactg tttgtagccg tgttcagcgg tagcaatcca
      781 aagcaataac gtattgatac cggaatacag acggttagaa accgcattgc gaggtaatgc
      841 catattgcat tccggatggc tccacggttt taaccacgga gctgtacctt gttccaatgc
      901 agcaatgatt ctatcagtaa tatgttggta taaatcaaat ttaggttgtt gagtgttcat
      961 catgtgtttc tccaaagttt gggtaaaaaa atggggaaac acggttaccc tatggggaac
     1021 ttgtttcccc gatgggtagt ttataaaatt tcaatggcca caatttttct agaatcatca
     1081 aagccaacag aactgcctat ggcgtagtaa ttacgaatag tctgttcatc ggcattgatt
     1141 agcagatacc tatcgaggaa gagagattgg gatgagaccg gtaacgagaa ataatcgtgg
     1201 aaccagtttt gaccctcgtc acaataaata catcaataca ttggcaaccg cattattggc
     1261 acgttggcat attgagcaat tctctgagcg taatatccca gtcaatgatg aaaatctcta
     1321 tatggctcac aatattggct tagatggttt ccatcgagcg ttattagggc aatctactcg
     1381 taaagacatt gagaatatgc gtaagaatgg gatgaaacct tggatgtcca tttagctctg
     1441 ttttcaaatg acctaatcca gtttgttccg gagagagtac cgaaattttg cactctttca
     1501 tccgtggtgt atcaaacttc aacgtgctaa atgagacatt aaatatacat caacataatc
     1561 taccgcactc atcgttaaga aaccgggatt cccatcagaa tgaggaatat cgttaaagcc
     1621 cgataaggag acggttaaac aaaatggtgt taaattactt gcttgatttc gtttcacagc
     1681 tctgctttct ccactcgctc gcccattcga aatacgaccg tctgcaaaca caaaggtaat
     1741 gctcgttacc tcagaacgaa cgcaagatag cgtccaatct ccggttgatg taccggatac
     1801 aatcgccccc tcaacatcag gtaaatcaat gccattggca attaaatttt ctcgcccgat
     1861 taatacttta aacggaaacg gatctgtcac agcaccttga ataggtacac ggcctaacaa
     1921 tgccgtcatc gcagtagaac ccattaaagt cgaattttcg ggaatagtgt aataaggtgt
     1981 acgctttatt tgctgtatct gagtattacc agtcatattt tctgtcccag cattattatt
     2041 taatgctgtc ccagtatttg aactttcccc ctctgcttta aaagggcttg gaaagccggt
     2101 ttttgttttc ccggaataat tgcctgtaac cggtttacct tgttcatcgg ttaccaccat
     2161 atcatcagga tcgacccagc gaatgccatt gttatctaca ttccctgttt gtcctgctga
     2221 ttcagtccca aagccagaag aagcccctcc tacagggatt gcctctgctt ctttctgctg
     2281 ctgattacca ctttgaaccc gacctttcag ttcttcgatt tggcgattaa atccatccca
     2341 agcagcagcc aaacggcttt ctacacgact ttctacttca cccgcaatac gagaatccac
     2401 ttcggcttct cttgcttgta agcgtaatac ctcttgctca aagcgatcac cacgctcatt
     2461 tgctttacga gcttcttcac gagtatcttt taatgctcct accaacgttt ttaatgtatc
     2521 gtgaggcgta tctcctgcag taatacctaa tgtattcgcc tcctcatcac t
//
LOCUS       DANLXQ010000231         2527 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.237, whole genome shotgun sequence.
ACCESSION   DANLXQ010000231 DANLXQ010000000
VERSION     DANLXQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.237"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            32..751
                     /locus_tag="PXD94_002554"
                     /pseudo
     CDS             32..751
                     /locus_tag="PXD94_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            811..1260
                     /locus_tag="PXD94_002555"
     CDS             811..1260
                     /locus_tag="PXD94_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL6287358.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
     gene            1361..1882
                     /locus_tag="PXD94_002556"
     CDS             1361..1882
                     /locus_tag="PXD94_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HDL6287359.1"
                     /translation="MYKAITLIEILTTITIMVIAVYFISPVIFTLQDRIALHSEIENI
                     QSFIQQIQTKARYTKRNYTLTLSQRNNQWCMIAVIKPINTSKQIICDCLNKHFCDLGK
                     EYHLYTPTHQQIVLKNKSLYPKSFINVDGVSGQLESKCIKLSLNKENEILQFEQLGRV
                     YVAAKNKRSTCKD"
     gene            1914..>2527
                     /locus_tag="PXD94_002557"
     CDS             1914..>2527
                     /locus_tag="PXD94_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287360.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVLKEIVGFKVENNEIYTFSSQLDNCVKEQCQTLLENCDGNWSKFTEA
                     NNFKTKKLDFSWRQEDNLLKIELEIESPKQKGLIYTAVRYVFIL"
BASE COUNT          875 a          410 c          499 g          743 t
ORIGIN      
        1 gttttagtcg ttatctagga cagcccctac ttttaatact cgtaagcccc cttttgataa
       61 ccctaaagtt cgtcaagcat tgaattatgc ggtgaataaa actgaaattt taacttccgt
      121 ttaccaaggc tctgcaacgc cagctaaaaa cccaatgcct tctacgattt ggggatataa
      181 caacgaagta gccggttatg aatttaatcc ggaaaaggcg aagagcttgc ttaaagaggc
      241 cggattagaa aatggctttg aaacagaaat ttgggcaatg cctgtttctc gcccttacaa
      301 tccaaatgcc cgtagaatgg ctgaaatgat tcaagaggat tggaaagcaa taggggtaga
      361 ggcaaagata gtgagctatg aatggggaga atatcttaaa cgaatgggaa acggcgagca
      421 cgctaccgga atgattggtt ggaccggaga taacggcgat ccagataatt tcctcaatgc
      481 cctactcagt tgtaatgcgg tagaacaagg ctcaaactat gcagggtttt gtaatccaga
      541 gtttgaccgt ttaattaatg aggctgcgac tatcagtgat caaaacaagc ggtcagattt
      601 atataaaaaa gcgcaattca tctttaatga gcaagcacct tggttaccga ttgcacattc
      661 aacggtgtat tttccggtaa ggaaagaggt aaaaggctat gttgtcagcc cgtttattgc
      721 tcataatttt tatggtgttg agctggaata acctaaggta taatgaattg aactcatttt
      781 gtgcctcata aatttaggag caataaatat atgttagcga aacttaagtg gaatcaatta
      841 gcttgtgtaa cattggcatt ttgttcagcc tcagtatttt cagcccatca cgaaatcaaa
      901 atgttagata acaatgcgga aggatcaatg gtatttgagc ccggttatgt aaaagcagag
      961 gtgggcgata cggtaacatt tattccgtct cataaagggc attgggttga aagccgctcg
     1021 attccggaag gtgcggagaa gttcttatct aaagaagatg aggaatttac tattaccctt
     1081 actcacgaag gtgtttatgt ttactactgt ccgccacacc gaaccatgaa tatggccgga
     1141 attatccaag taggacaagc tctcaatctt gaagccacac aaaaagaagt ggaaaaaatt
     1201 gaaaaacgca cgaaagaaaa taaaggacgt ttatttgaat atttagagca agttaagtag
     1261 aagtgattta attttgcgat ctgcatcgaa aaatcaatat ttaatagttt acaaatgatg
     1321 cttaaattct aagataccct ctatcaatga gggtattttt atgtataaag ccatcacatt
     1381 aattgaaata ctaaccacta tcactattat ggtgatcgct gtttatttta tctcgccggt
     1441 tatttttaca ctgcaagatc gaattgcact tcatagtgaa attgaaaata tccaatcttt
     1501 tattcagcaa atccaaacta aagcacgtta taccaaacgt aattatacct taaccctttc
     1561 tcagcggaat aatcagtggt gtatgattgc ggtaattaag ccaatcaata ctagcaaaca
     1621 aattatttgt gattgtttga ataaacattt ttgtgattta ggaaaagaat atcatcttta
     1681 tacgccgact catcagcaga ttgtattaaa aaataaaagt ttatatccga aatcctttat
     1741 taatgttgat ggggtaagtg gtcaattaga atcaaaatgt attaagctca gtttaaataa
     1801 agaaaatgaa attttgcaat ttgaacaatt aggacgggtt tatgtggcag caaaaaataa
     1861 acgtagcacc tgcaaagatt agtttatctg cttttagcct aattgaagta ttaatggcta
     1921 gtgcattagg ggcgttttta ttattgcttg ttgcttcaag ttatgccgat ttctattaca
     1981 cacaaaccaa acaaaaagaa cagctctact tgcaagcaga agctcaccag ttactaaact
     2041 acttccaaca gcattttcaa cacattggct atcaaggctc tcacagaaca gacagtaatt
     2101 ttgatttatt tcagcaagca gggaaaagcc tgaatattgt gcataaaaat tgcattattg
     2161 gattttacga tttaaaccaa gatggctgtt tgggaaaacg taagacaaaa acggccgctt
     2221 gtaagttagg aacagtgaat aataccagag aggtgttaaa agaaattgtt ggctttaaag
     2281 tagaaaataa tgaaatttat actttttcat cgcaattaga taattgtgtg aaagaacaat
     2341 gccaaacatt attggaaaat tgcgatggaa attggagtaa atttaccgaa gcaaataatt
     2401 tcaaaactaa gaaactggat tttagttgga gacaggaaga taacttatta aaaattgagt
     2461 tagaaataga atcacctaaa caaaaaggat tgatttatac ggcagtacgt tatgttttta
     2521 tcttaaa
//
LOCUS       DANLXQ010000232         2514 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.238, whole genome shotgun sequence.
ACCESSION   DANLXQ010000232 DANLXQ010000000
VERSION     DANLXQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.238"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            21..362
                     /gene="erpA"
                     /locus_tag="PXD94_002558"
     CDS             21..362
                     /gene="erpA"
                     /locus_tag="PXD94_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL6287361.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
     gene            complement(467..790)
                     /gene="yidD"
                     /locus_tag="PXD94_002559"
     CDS             complement(467..790)
                     /gene="yidD"
                     /locus_tag="PXD94_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL6287362.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(739..1113)
                     /gene="rnpA"
                     /locus_tag="PXD94_002560"
     CDS             complement(739..1113)
                     /gene="rnpA"
                     /locus_tag="PXD94_002560"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL6287363.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKPAQQNP"
     gene            complement(1149..1283)
                     /gene="rpmH"
                     /locus_tag="PXD94_002561"
     CDS             complement(1149..1283)
                     /gene="rpmH"
                     /locus_tag="PXD94_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL6287364.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            1520..2482
                     /gene="hemH"
                     /locus_tag="PXD94_002562"
     CDS             1520..2482
                     /gene="hemH"
                     /locus_tag="PXD94_002562"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL6287365.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDQTLESFPQKGIKKVAVVCPGFSADCLET
                     LEEITEENRENFEHAGGESYRYIPALNASGDHIKALVKLIEAKI"
BASE COUNT          759 a          484 c          550 g          721 t
ORIGIN      
        1 aaattttgat ctaaaacaaa atgagcgata ttcaaattcc tttaatcttt actgatgcgg
       61 cggctcgtaa agtaaaaagc ttaattgagg gcgaagagaa cccgaatctt cgcttgcgtg
      121 tgtatattac gggtgggggc tgtagtggat tccagtatgg gttcaccttt gatgatcaag
      181 tcaatgaggg agaccttacc attgagaatc aaagcgttgg cttggtggtt gacccgatga
      241 gcctacaata tttaattggt ggcacggtgg attataccga agggctggac ggctcacgtt
      301 ttgtcgtgaa taatccaaat gcaagctcaa cctgtggttg tggttcttcc tttagcattt
      361 aattcactcg aattattgcc ttattcaata tcagtctcac aagtaaattt tttactaaaa
      421 atgaccgttt gtattaaaca aaatagccta acttattgag tttaggctat tttttatcat
      481 tatgtttgca gttgccttta caacaaagtg ggacaggatc ttcgcctccc tcactcaaag
      541 ggttgcaacg cagaatacgc ttaaacgcta aatacccacc tttaattgct ccgtggattt
      601 taatcgcttc gaccgcatag gtggagcagg ttgggtaaaa tcggcaacgt gggccaatca
      661 atgggctgat agcatagcga tagaaataaa tcggtagcaa tagaattttg gctaataggc
      721 tgagacgagg tggctgattt atggattctg ttgtgccggc tttttgctca ggcgaatgtg
      781 tcttgaccat agtttttcca atgtttcaaa cagcgttgcg ttgtctaatt tgccaatgcc
      841 gccttttgcc acaaatacaa aatcgaaatt cggcaattcg tgttgcttga ggcgaaaact
      901 ttcacgcact atccgcttaa tgcgattacg gtcgtgagcc cgttttaaat gttttttggc
      961 aacagttaag cctagacgag gattttctag attattctct ctagcaagaa gagtcatttg
     1021 gggtgtgctg gctcgaagtg gctcttcaaa caccgcctta aattgaacgg gagctaacaa
     1081 acgtagctcc cgagagaaat ttagcttctt cacacttaat cagaaaggtg ttttggctgg
     1141 ttctgcaatt atgcagataa agatttacga cctttagcac gacgacgcgc taacacttga
     1201 cgaccgtttt tagtagccat acgagcacgg aaaccgtgag tacgagcacg ttttaatact
     1261 gatggttgaa atgtacgttt cattgcttat ctacctaaaa tttaaaaagt tggcataaaa
     1321 tggtttctgc caaacaagga ccataaaaaa gagttgcaat tctacaggca attaaaataa
     1381 aaagcaagaa acaatccaag cacaactcgg gttgtttgca aaatttttga aaaatacgac
     1441 cgcttgttat aatcgcagaa atttatattg gtttgtggca taatcgaaaa aatattaact
     1501 attgatgaga aatagaacaa tgaatcaacc ccaacaaata ggtattttgc tggctaattt
     1561 aggtacgcca gatgaaccca cgccaagtgc ggtgaagcgt tatttaaagc aatttttaag
     1621 cgatcctcgt gtgatcgatc taccaaaatt taaatggcaa gcaatcctaa actgcatcat
     1681 tttgccaaga cgttcgccaa aagtggcaaa attatatcaa tcagtatgga gcgaagaagg
     1741 ctcaccgtta ttggcgattt ctcggcaaca acaacgagcg gtacaacgct attttgatgc
     1801 caaaggcaaa aatgtggtgg tggaattggg aatgtcttac ggcaatccaa gtattgaaag
     1861 tgcgaccgat aacctcatca agcagggggt tgagcggatt attgtgctac cgctttatcc
     1921 gcaatacagt agcactacta ccgcttcggt gtttgatgcg tttgccaacg gtttaaaagc
     1981 acagcgtaaa atcgtgccgt ttgattttat tcacagctat cataacgatc cgctttatat
     2041 tcaagcgttg gctaactcta ttgatgttaa acctgacgag ttattactgt tttcatttca
     2101 cggcattcct aaacgttatg aaacggaagg tgatttttac gccaagcatt gccaagaaac
     2161 agcagaatta gtggctgaaa agctaggtct gccaagagaa aaatggtatt tgtctttcca
     2221 gtcccgtttt ggtgatgaag agtggctaca gccttatacc gatcaaaccc ttgaaagctt
     2281 cccacaaaaa gggataaaaa aagtggctgt cgtttgcccg ggcttttcag cagattgttt
     2341 ggaaacctta gaagaaatta cagaagaaaa ccgcgagaac tttgaacatg ccggcggaga
     2401 atcttatcgc tacattccag cgttaaatgc gagtggcgat catatcaagg cattagtaaa
     2461 attaatcgaa gcgaaaatat aatataattt gggcggataa taaatccgcc catt
//
LOCUS       DANLXQ010000233         2487 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.239, whole genome shotgun sequence.
ACCESSION   DANLXQ010000233 DANLXQ010000000
VERSION     DANLXQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.239"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            8..1123
                     /gene="wecB"
                     /locus_tag="PXD94_002563"
     CDS             8..1123
                     /gene="wecB"
                     /locus_tag="PXD94_002563"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDL6287366.1"
                     /translation="MRYLIVFGTRPEAIKMAPLVEGFKKSGLNFKVCVTAQHRQMLDQ
                     VLELFEITPDYDLDIMSNKQTLSTVTSAILEKIQPVIAEYKPTTIFVHGDTATTLASS
                     LAAYYNQIDIAHIEAGLRTHNIYSPWPEEGNRKLTAALAKYHFAPTQSTKKNLLNENI
                     AEGTIYVTGNTVIDALFLACKKLDENIDLLSSLQEKFSYIKSKRTILITGHRRENFGN
                     GFENICKAVSTLASDFPDVQFIYPVHLNPNVREPVNRLLADKSNVHLIEPCDYLSFVY
                     LMRESYLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVKLVGTAQSEIIKQV
                     TELLNDQAAYTKMSEAHNPYGDGTAVKQILSVFQSKQ"
     gene            1138..2409
                     /gene="wecC"
                     /locus_tag="PXD94_002564"
     CDS             1138..2409
                     /gene="wecC"
                     /locus_tag="PXD94_002564"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL6287367.1"
                     /translation="MSNFNTISVVGLGYIGLPTATVFAQHGLNVIGVDVNQHAVDHIN
                     QGKIHIVEPDLDVAVHQCVSSGKLKATLTPEPAEAFLIAVPTPFKGEDYEPDLSYIQA
                     ACKAIAPVLEKGNLVILESTSPVGATEQMAEWLAEYRPELSFPQQAGEDSDIRIAHCP
                     ERVLPGQVMHELIENDRIVGGMTNKCSVQAVNLYKTFVKGDCIVTNARTAEMCKLTEN
                     SFRDVNIAFANELSIICDRLDINVWELIALANRHPRVNILQPGCGVGGHCIAVDPWFI
                     VNKTPDLAKIIRTAREVNDHKPEWVISKMNEAVIYALQKTGKSIDQIKIACLGLAFKP
                     DIDDLRESPALKITEKLAEKYPNQVFAVEPNVEELPKKLANKNISLISVDEALEVADV
                     VVVLVDHSEFKLVKPVFLNDICVVDTKGIWR"
BASE COUNT          801 a          483 c          490 g          713 t
ORIGIN      
        1 gtaataaatg cgctatctca tcgtcttcgg tactcgtcct gaggcaatca aaatggctcc
       61 cttagttgag ggctttaaga aaagcggtct aaattttaaa gtctgtgtta ctgctcagca
      121 ccgccaaatg ctggaccaag ttttagaatt attcgaaatt actccagatt atgacctgga
      181 tattatgagc aataagcaaa cactttctac tgtaacttct gctattttag aaaaaattca
      241 acctgttatt gccgaataca aaccaactac tatttttgtt catggcgata cagcaaccac
      301 tcttgcctct tcattagcag cctattacaa ccaaattgat atcgctcata ttgaggcagg
      361 tctaagaacg cataatatct actctccttg gccggaagaa ggcaatcgca aattaacagc
      421 tgctttggca aaataccatt tcgcaccaac acaaagcacc aagaaaaatc tactcaacga
      481 aaatattgct gagggcacca tttatgtaac gggtaatacg gtaattgatg ccttattcct
      541 cgcttgcaaa aaattagatg aaaatattga tttattaagc tcgctacaag agaaattttc
      601 ttatataaaa agtaagcgta ctattttgat caccggacat cgccgcgaaa atttcggtaa
      661 tggttttgag aacatctgta aagccgtttc aacattagcg tctgatttcc ctgacgttca
      721 atttatctac cccgtacacc tcaacccaaa tgtaagagaa ccggtcaatc gcctattagc
      781 ggataaatcc aatgtgcatc tcattgagcc ttgcgactat ctctcctttg tttatttaat
      841 gagagagtct taccttatct taactgactc cggaggtatt caagaagaag ccccatcatt
      901 aggaaaaccg gtattagtaa tgagagacac aacagaaaga ccggaagctg tagaagccgg
      961 aacagtaaaa cttgttggaa ctgcacaatc tgaaattatt aaacaagtta ctgagttact
     1021 caatgatcaa gctgcttata caaaaatgtc tgaggctcac aacccttatg gcgatggcac
     1081 agctgtgaaa caaattctct cagtatttca atcaaaacaa taataaggga taaatgtatg
     1141 tcgaacttta ataccatttc agtagtaggc ttaggctata ttggcttacc aaccgccact
     1201 gtatttgccc aacacggtct aaatgtaatt ggcgtagatg ttaatcagca cgccgtagac
     1261 catatcaatc aagggaaaat tcacattgtc gagcctgatt tagatgttgc tgttcaccaa
     1321 tgtgtatcta gcggaaaatt aaaagccaca ctgacacctg aaccagcaga agcattttta
     1381 attgctgtac caacgccttt taaaggcgag gattatgagc ctgatttaag ttatattcaa
     1441 gctgcttgca aagcaattgc acctgtgctg gaaaaaggta atttagtcat tttagaatca
     1501 acctctccgg ttggtgcaac agaacagatg gctgagtggc ttgccgagta ccgtccagag
     1561 ctaagcttcc ctcagcaagc aggtgaagat tctgatattc gtatcgccca ttgcccagaa
     1621 cgagttctac caggacaagt gatgcatgaa ttgattgaaa acgaccgtat tgtgggtggt
     1681 atgacaaata aatgctctgt gcaagcggtc aatttataca aaacttttgt aaaaggcgac
     1741 tgtatagtta ccaatgcccg taccgcagaa atgtgtaaat taaccgaaaa tagtttccgt
     1801 gatgtcaata tcgcttttgc aaatgagctt tctattattt gtgatcgcct agatattaat
     1861 gtatgggaac taattgcttt ggctaacaga caccctcgag tcaatatctt acaaccgggt
     1921 tgtggtgtag gtggacactg cattgctgta gatccttggt ttattgtgaa taaaactcct
     1981 gacttggcaa aaattatccg cactgctcgt gaggtaaacg atcataaacc tgaatgggtt
     2041 atctcaaaga tgaatgaagc ggtaatttat gccttacaaa aaacaggtaa atctatcgat
     2101 caaatcaaaa ttgcttgttt agggttggca ttcaagcctg atattgacga tttgcgtgaa
     2161 agtccggcat taaaaattac tgaaaaattg gcggaaaagt atccaaatca agtatttgca
     2221 gttgagccga atgttgaaga gctaccgaaa aaattggcta acaagaacat ttctttgatt
     2281 tctgttgatg aagctctgga agtagctgat gttgtagttg ttttggtgga tcatagcgaa
     2341 tttaaactag tcaaacctgt gtttttgaat gatatttgtg tggtggatac taaaggaatt
     2401 tggagataag tgatgaaaaa gaaacagcaa tatcgtaaat ccactattaa caatactaat
     2461 gcatcacaac aaaattcagg cgaagta
//
LOCUS       DANLXQ010000234         2463 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.240, whole genome shotgun sequence.
ACCESSION   DANLXQ010000234 DANLXQ010000000
VERSION     DANLXQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.240"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            29..1504
                     /gene="msr(E)"
                     /locus_tag="PXD94_002565"
     CDS             29..1504
                     /gene="msr(E)"
                     /locus_tag="PXD94_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HDL6287368.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     gene            1560..2444
                     /locus_tag="PXD94_002566"
     CDS             1560..2444
                     /locus_tag="PXD94_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="HDL6287369.1"
                     /translation="MTIQDIQSLAEAHGLLITDKMNFNEMGIDFKVVFALDTKGQQWL
                     LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAE
                     TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV
                     KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH
                     IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL
                     ETQNESLIVGAKAQLGVI"
BASE COUNT          841 a          406 c          532 g          684 t
ORIGIN      
        1 ttcaagagct aaacaggagt aaataaaaat gagtttaatt attaaagcga gaaacatacg
       61 cttggattat gctgggcgtg atgttttgga tattgatgaa ttggaaattc actcttatga
      121 ccgtattggt cttgtgggtg ataacggagc aggaaagagt agtttactca aagtacttaa
      181 tggcgaaatt gttttagccg aagcgacatt acagcgtttt ggtgattttg cacatatcag
      241 ccaactgggc ggaatcgaaa tagaaacggt cgaagaccgg gcaatgttat ctcgccttgg
      301 tgtttccaat gtacaaaacg acacaatgag tggcggagag gaaactcgtg caaaaattgc
      361 tgccgcattt tcccaacaag tacatggcat tctagcggat gaaccaacca gccaccttga
      421 tctcaatgga atagatctac ttattggtca acttaaagca tttgatggag cattacttgt
      481 tatcagtcat gaccgatatt ttcttgatat ggttgtagac aagatatggg agttaaaaga
      541 cggtaaaatt acggaatatt ggggtggtta ctcggattac ttgcgtcaaa aagaagaaga
      601 gcgacaacac caagccgtag aatatgagct gatgatgaag gaacgggagc gattagaatc
      661 tgctgtgcaa gaaaaacgcc agcaagctaa tcgattagac aataagaaaa aaggagaaaa
      721 atccaaaaac tctaccgaaa gtgctggacg acttgggcat gcaaaaatga ctggcaccaa
      781 gcaaagaaaa ctgtatcagg cagctaagag tatggaaaag cgtttggctg cattagaaga
      841 tattcaagca ccagagcatt tgcgttctat tcgttttcgt caaagttcag ccctagaact
      901 gcacaataag ttcccgatta cggcagatgg tctgagctta aaatttggta gccgtactat
      961 ctttgatgac gctaacttta taataccgct tggcgctaaa gtcgctataa ctggatcgaa
     1021 tggaacaggg aaaacgtcct tgttaaaaat gatatcagaa cgtgctgatg gattaaccat
     1081 atctccaaaa gctgaaattg gctactttac acaaacagga tataaattta acacgcataa
     1141 atctgtgctc tcctttatgc aggaagagtg cgagtacaca gttgcggaaa ttcgtgcagt
     1201 attggcttca atggggatcg gagcgaatga tattcaaaaa aacttatccg acttatcggg
     1261 aggtgaaatc atcaaactgc ttttatccaa aatgctttta ggaaaatata atattttgct
     1321 tatggatgaa ccaggaaact atcttgacct aaaaagtatt gccgcattag aaacaatgat
     1381 gaagtcctat gcaggaacta ttatcttcgt atctcatgac aagcaattgg tcgataatat
     1441 tgctgacatt atctacgaga tcaaagacca caaaatcatc aagacttttg agagagattg
     1501 ttaatgatag ccaatctaat ccgaacatta attattgaac tctttaaagg aaattaaaaa
     1561 tgacaattca agatattcaa tcacttgctg aagcacacgg cttgttgatt acggacaaaa
     1621 tgaatttcaa tgaaatgggc attgatttta aggtcgtttt tgctcttgat acaaaggggc
     1681 aacaatggtt gctgcgtatt cctcgtcgtg atggcatgag ggaacaaatc aagaaagaaa
     1741 aacgcatttt agaattggta aaaaaacatc tttctgtaga ggttcctgat tggagaattt
     1801 catctacaga attagtggct tatcccatac ttaaagataa tcctgtttta aatttggatg
     1861 ctgaaaccta tgaaataatt tggaatatgg acaaagatag cccgaaatac ataacatctt
     1921 tggcaaaaac cttatttgaa atccatagta ttcctgaaaa agaagttcgg gaaaatgatt
     1981 tgaaaattat gaaaccttca gatttaagac ctgaaatagc aaacaatttg cagttagtaa
     2041 aatctgaaat tggtataagt gagcaattgg aaacccgcta cagaaaatgg ttggataatg
     2101 atgttctatg ggcagatttc acccaattta tacatggcga tttatatgct gggcatgtac
     2161 tagcttcaaa ggatggagct gtttcaggcg ttattgattg gtcaacagcc catatagatg
     2221 acccagcgat tgattttgct gggcatgtaa ctttgtttgg agaagaaagc ctcaaaactc
     2281 taatcatcga gtatgaaaaa ctagggggta aagtttggaa taaactatat gaacagactt
     2341 tagaaagagc agcggcctct cctttgatgt atggtttatt tgccttagaa actcaaaatg
     2401 aaagccttat cgttggagca aaagctcagt tgggagttat ataatttaaa aatatgattg
     2461 ctg
//
LOCUS       DANLXQ010000235         2460 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.241, whole genome shotgun sequence.
ACCESSION   DANLXQ010000235 DANLXQ010000000
VERSION     DANLXQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.241"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..403)
                     /locus_tag="PXD94_002567"
     CDS             complement(<1..403)
                     /locus_tag="PXD94_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL6287370.1"
                     /translation="MLEKIEKLIDEINKLHLAFSKDYFETGKIEKVNLKHTFAKVPTP
                     HILAYRLNLHESINDYLYRADLEGISYFYRVKTSESILDKIERFKQRSEGYPVNSILN
                     DIFGARIIVSSEVIQQVMESLDYWQEKYGLKN"
     gene            complement(424..1695)
                     /locus_tag="PXD94_002568"
     CDS             complement(424..1695)
                     /locus_tag="PXD94_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL6287371.1"
                     /translation="MSIQQKKRHSRWYERIFFSTAVQSILGVMFSSVLPAIYFWGEKF
                     NSIETRSNTIIATSLAFVMIAISLRVLMKYPGNKSASFVFSTVISWYAVLLIVLTIFR
                     FDYSNTLLGTSFILTILFCFIGYFLSRSWIIPKIALVPFGRAINLNRIQNVEWIQLSE
                     PILGEKRINSIAVDLHYPDLSAEWHKFLAKCTLNGIPVYNVRQLEESLTGRVKIRHMY
                     ENDLGSLLPSPIYSLVKRIIDIALIVATFPITLPIMTITAIAIRLESEGGAMFIQKRV
                     GQGGKEFNIYKFRSMCKDSEKDDAQFASSADMRVTRIGKFIRKTRIDELPQFFNVLKG
                     DMSLIGPRPEQTAFVDKFEEEIPFYNYRHIVKPGISGWAQVVQGYAADVDDTKVKVEH
                     DFYYIKHFSLWLDILIIFKTLKTMLTGFGAR"
     gene            complement(1699..2460)
                     /locus_tag="PXD94_002569"
                     /pseudo
     CDS             complement(1699..2460)
                     /locus_tag="PXD94_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          844 a          483 c          357 g          776 t
ORIGIN      
        1 aattttttag tccatatttt tcttgccaat aatctaatga ctccattact tgctgaatca
       61 cttcactaga aacaataata cgagctccaa aaatatcgtt gagaatgcta tttactggat
      121 aaccttcact acgctgttta aaacgctcga ttttatctaa aatcgattct gaggttttta
      181 ctcggtaaaa ataagaaatt ccctctaaat ctgcacgata taaataatca ttaatagatt
      241 cgtgtaaatt aagacgataa gctaaaatat gtggcgtagg cactttggca aaagtatgct
      301 taagattcac tttttcaatc ttacctgttt caaagtagtc tttcgaaaaa gctaagtgca
      361 acttgttgat ttcatcaatg agtttttcta ttttctcaag catgctattc actctctaat
      421 ttattatctt gccccaaatc cagttagcat tgttttcaat gttttaaaaa taattaaaat
      481 atcaagccat aaagaaaaat gcttaatata gtagaagtca tgttctactt ttacttttgt
      541 atcatcaaca tctgccgcat aaccttggac gacctgagcc caaccggaaa tacccggttt
      601 aacgatatgg cgataattat agaatggaat ttcctcttca aatttatcta caaaggctgt
      661 ctgttctggg cgaggaccaa ttaagctcat atcacctttt aatacattaa aaaactgagg
      721 tagctcatca atacgagttt tacggataaa tttcccaata cgagttaccc tcatatccgc
      781 actcgaagca aactgtgcat catctttttc ggagtcttta cacatactac gaaatttata
      841 aatattaaac tcctttccac cttgcccaac gcgtttttga ataaacattg ctccaccttc
      901 actttctaag cgaatagcaa tagcggtgat tgtcataata ggtaaagtaa tgggaaacgt
      961 agcaacaatc aatgcaatat caatgatacg cttcactaat gagtaaattg gagaaggaag
     1021 taatgaacct aagtcatttt catacatatg gcgaattttg acacgcccgg ttaaagattc
     1081 ctcaagctgg cgaacgttat atacaggaat accattcaat gtacatttcg ctaaaaattt
     1141 atgccattct gcggacaaat caggatagtg taaatctacc gcaatagagt taattctctt
     1201 ttcccccaat attggttcac ttaattgaat ccactctaca ttctgaattc ggttcaaatt
     1261 gatagctctt ccgaatggaa ccaaagcgat tttagggata atccaacttc ttgaaagaaa
     1321 ataaccaata aaacaaaata gaattgtgag tataaagctc gtacctagca atgtattaga
     1381 ataatcaaat ctaaatattg ttaacacgat taataaaacc gcataccaag aaataacggt
     1441 ggaaaaaaca aaacttgctg acttattacc tggatatttc attaacactc ttaagctgat
     1501 agctatcatt acaaaggcaa gagaagtcgc tataatcgta ttagaacgag tttcaataga
     1561 attaaatttc tctccccaaa aatagatagc gggtaagaca cttgagaaca taacgcccaa
     1621 aatactttga acagctgtac taaaaaagat acgttcatac caacgagagt gtcttttctt
     1681 ttgctgaatg ctcataattt atccttttct taatttctta taaagtcttc ctaaaagaga
     1741 tctaggcaat aaccgtggta aggctctaag tataaaaaac atgcttgatg agattattga
     1801 ttgtaatccc aactcttttt tcaattttgc taattggtac tcactcttaa tgtaattcca
     1861 ccctttacgc cgagcgtaca ttgcagaacc tgaacgaaca tttaccagta catctggtaa
     1921 attatacacc tcatatttct gtgtaataac tcgtaaccat aaattgtaat cttccatata
     1981 aagatgatgc tgatagcctc caacatgctc gatcacagat tttttatagg ctactgccat
     2041 atggttaaaa ggatttctta gtaatgctga aatacgaatt tcatcatcat taatgggcac
     2101 ttgttttata cccagagaat tagacattgt ttcgtcaaat tcttcaactt gtccccccaa
     2161 taaaaccaca tcaggatttt tactaataaa atcaatttgc ttttcaaatc gagatggtaa
     2221 gcaaatatca tcagtatcca ttctaaacac ccactcatta gagcagtgtt ttaagccttc
     2281 atttaaggcc ggcctaaacc cacattttgt ggtaaactaa caacaacaag cggtaatttt
     2341 tcagtaaaat gttgcacaat tcgctctaaa tctccaggaa ttataccatc aaaaaccaca
     2401 acaatttcac ttgctggtaa agtttgggat actaaagaat ctaaacacat ctctaaataa
//
LOCUS       DANLXQ010000236         2441 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.243, whole genome shotgun sequence.
ACCESSION   DANLXQ010000236 DANLXQ010000000
VERSION     DANLXQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.243"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1067
                     /gene="hisC"
                     /locus_tag="PXD94_002570"
     CDS             <1..1067
                     /gene="hisC"
                     /locus_tag="PXD94_002570"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL6287372.1"
                     /translation="TISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDLT
                     DRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYCP
                     PTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIKR
                     SDLFELLQITAGKAIVVVDEAYIEFCPEARLVSELANFPHLAIIRTLSKAFALAGLRC
                     GFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQK
                     NLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITVG
                     AREENLRVVEAITALSQFQR"
     gene            1252..2340
                     /gene="hisB"
                     /locus_tag="PXD94_002571"
     CDS             1252..2340
                     /gene="hisB"
                     /locus_tag="PXD94_002571"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL6287373.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
BASE COUNT          679 a          614 c          595 g          553 t
ORIGIN      
        1 tgacaatttc acaactttcc cgtaaaaaca ttcaagctct caccccttac caatcggcac
       61 ggcgtttggg gggtagtggc gatgtgtggc tgaatgccaa tgaatatgcc ctttcgccaa
      121 actttgattt aaccgaccgc acttttaacc gctaccctga acctcaaccg caggcggtga
      181 ttgaaggtta cgcccgatat gcaggcgttg cacctgaaaa cgtgctggtt tcccgtgggg
      241 gcgatgagag cattgagctg attatccgag ccttttgcga ggcaaacgac agcattctct
      301 actgcccacc aacctacgga atgtacgccg tgagtgccga cacctgtggc attgcgacca
      361 aaactgtgcc tttaaccgcc gatttccagt tggatttagc ccaaatcaaa gctaacctcg
      421 agggcgtgaa agtggtgttt gtgtgcagcc cgaacaaccc gacaggcaac ttaatcaagc
      481 ggtcggattt gttcgaactt ttgcaaatca ccgcaggcaa agcgattgtg gtggtggacg
      541 aagcctacat cgaattttgc cctgaagctc gcttggtaag cgagctggcg aatttcccac
      601 atttggcgat tatccgcacc ctatccaaag cctttgcctt ggcgggtttg cgttgtggct
      661 ttaccttggc aaacgctgag ctgatcggcg tgttacaaaa agtgattgcc ccgtatccgc
      721 tgcctgtgcc tgtttccgac atcgccgccc aagccctctc cccacaaggc attgagcaga
      781 tgaaaacccg tgtggcggaa gtcatcaccc tgcgtgccga attgcaaaaa aatctggaaa
      841 acttaccgct tgtagaacaa gtttttgaca gcgaagccaa ctacctccta ttcaaatgcc
      901 aagacggaca aaaagtcttc aaagcacttt gggagcaagg cattattttg cgtgaccaac
      961 acaaagcgtt aggattgcaa aattgcattc ggattacggt gggggcgaga gaggagaatt
     1021 tgagggtggt tgaggcgatt acagcactga gccaatttca gagatagaat ttaacgagca
     1081 gtattttaag agacatttat agcctagcac ggcatagccg tactaggtta cgcataagtc
     1141 agtccctttg ggactgaata cagagaggca aagtctctca ctaatgctta accccatatg
     1201 ccttggcgtg tggggagagt aatgaataag atacagatac aggagaaacc aatgcaaccc
     1261 acccttttta tcgaccgtga cggcacgtta attgatgagc caaaaaccga ttttcaaatc
     1321 gacagcttgg aaaagctgaa attcgagaaa aacgtgattc ctgccctgct caaactcaag
     1381 ggcaaatacc gctttgtgat ggttacgaac caagacggct taggcacgag ttcgttccca
     1441 caggcagatt tcgacaaacc gcacaatgca atgatggcgt tgttcaactc gcaagggatt
     1501 gaatttgatg atgtgctgat ttgtccacac aagcccgaag acggttgcga ttgccgcaaa
     1561 ccgcacacca aactgctaca aaaatatatc gaccgccaac tgttcgaccc agccaacagc
     1621 tttgtgattg gcgaccgtgc taccgatgtg caactggcgg aaaaccttgg cattcgggcg
     1681 ttgcaatatc accctgaaaa actgaactgg gatttgattg ctgaaaaact actgggcgaa
     1741 gccgtcacca acattggcga gcgaccaaag cgctttgccg aagttatccg taaaaccaaa
     1801 gagaccgaca tcaaagtgca agtgtggctg gacgaaacag gcaacaacga aatcagcacc
     1861 ggcgtgggct ttttcgacca tatgcttgac caaattgcca cccacggcgg ttttagaatg
     1921 aacatcacca ccaaaggcga cttgtggatt gacgaacatc acacggtgga agacaccgcc
     1981 ctcgccctcg gcaccgcatt aaaacaagcg attggcgaca aacgcggcat cgcccgtttc
     2041 ggcttcgtgt tgccgatgga cgaaagccgt gcggaatgtg cgatggactt atcaggcaga
     2101 cctttcatca agttcaaagc ggatttcaaa cgcgacaaag tgggcgattt cagcaccgag
     2161 ctcaccgaac acttcttcca atccatcgcc ttcacgatgc tcgccaccct gcacatcaag
     2221 gcaaaaggcg acaacgacca ccacaaaatc gaaagcctgt tcaaagtctt cggcagaaca
     2281 ctcagacagt gcattcgtat tgaagggaat gaactaccaa gcagtaaagg tgtattataa
     2341 tcaccgtagg ggcgggtttt aacccgcccg aaagtgcggt tgaaatttgc aaagtttttg
     2401 gggattttga ccgcttgtta taaaggatat attgatgatt a
//
LOCUS       DANLXQ010000237         2428 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.244, whole genome shotgun sequence.
ACCESSION   DANLXQ010000237 DANLXQ010000000
VERSION     DANLXQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.244"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(116..191)
                     /locus_tag="PXD94_002572"
     tRNA            complement(116..191)
                     /locus_tag="PXD94_002572"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PXD94_002573"
     tRNA            complement(195..269)
                     /locus_tag="PXD94_002573"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PXD94_002574"
     tRNA            complement(313..397)
                     /locus_tag="PXD94_002574"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
     gene            complement(432..507)
                     /locus_tag="PXD94_002575"
     tRNA            complement(432..507)
                     /locus_tag="PXD94_002575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(472..474),aa:Thr,seq:tgt)
     gene            754..1704
                     /gene="coaA"
                     /locus_tag="PXD94_002576"
     CDS             754..1704
                     /gene="coaA"
                     /locus_tag="PXD94_002576"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL6287374.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1857..2396
                     /locus_tag="PXD94_002577"
     CDS             1857..2396
                     /locus_tag="PXD94_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL6287375.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQNIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYDGLK"
BASE COUNT          757 a          509 c          465 g          697 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaattcag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcgctc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtcag
      481 ttgctctacc tactgagcta agtcggcgtt gctgtgtcgt aagcaagtga tgtgcattat
      541 agggaaattt taacagcgtg caagtgtttt ttaaaaaaat attaaaaaaa gcgttctttc
      601 gatttttaaa tatacaaaat gactattttt tgtaaaatac aacggatttt ctctgcataa
      661 ctacggcaaa ataagtgcat atattgtata gttacgccct taatttggta tttaattacc
      721 ttaatattcc agcaaaaaat tgagattttg gctatgagcc ttgaaaaaac cagcaaaatt
      781 actccttttc tcacttttga tcgccaacaa tgggctgatt tacgcaaatc cgttccattg
      841 aagctgaccg agcaagacct aaaaccgctt ttaggcttta atgaagatct ttcattagaa
      901 gaagtacgca ctatttattt accgcttgcc cgtttaattc aatactatat agaagaaaac
      961 ttaaaacgcc agacagtctt acatcgcttt ctcggcattg aaccgccaaa agtcccttac
     1021 attattagta ttgccggcag cgtatcggtt gggaaaagta cctctgcccg tattctgcaa
     1081 gctctgctct cccattaccc taaagagcga aaagtggatt taattaccac cgacggtttt
     1141 ttatatccac ttgcaacgct aaccgaaaag aacttactca agaaaaaagg ctttccggaa
     1201 tcttatgata ttcacagcct gattcgcttt gtatctgata ttaaatccgg caaacgcaat
     1261 gtgaaagctc cgatttattc acatttgacc tacgatatta tcccaaatca atttaatacg
     1321 gtggatcaac ccgatattgt gattttagaa gggctaaacg tgttacaaag tggaatgaac
     1381 tacccgtcca gcccacataa tgtatttgta tcggactttg tggatttttc tatttatgtg
     1441 gatgccgaag aaagtttgct gaaaaaatgg tatatcaacc gtttcttgaa attccgccgc
     1501 agtgcctttg ccgacccaaa ctcctatttc caccattatg cgagtttgtc cgagcaagaa
     1561 gcgattgaaa ccgcctcaac gatttgggaa gagatcaacg gcttaaattt acgccaaaat
     1621 attctcccaa gccgagagcg tgccaacctc attttaatca aagggcaaga ccacgccatt
     1681 gaaacggtaa aattacgcaa ataatatttt gcaaataatt gggctttttc gaccgcttgt
     1741 aataccttaa aatccgttga ataccactca acggattttt tatttaaatc aatgcttatt
     1801 caactatttt ttggtaagct aacgactttg agagtgattg atattaggca tatacaatga
     1861 acaaaaaatt tctacttcct ttaattctct ttttggcgtt atctgtggca tttttggttc
     1921 aactcgctcg caactcgcaa ggcgatgatc cgaaaaaatt agaatcagcc ttaattggca
     1981 aaacagttcc gagctttaat ttagaatcgg tattagatga aaaacagaca atcgaccaaa
     2041 atatcgtcaa aaccggcaaa cctcgcctgc ttaacgtgtg ggcaacttgg tgtccgactt
     2101 gttatgccga acacgaatac ttaaccattt tggcaaaaca aggggtggaa attgtcggta
     2161 ttgattataa agacgaacgc ccaaaagcga ttaaattctt agccaattac ggcaaccctt
     2221 ataaagcgat tatttttgac ccgaaaggct cactcggttt agatttaggt gtttatggtg
     2281 cacccgaaac ctttattatt gacggcaacg gcgtgattca ataccgccac gccggcgatg
     2341 taaacgacca agtgtggaac caagtgctta aaccgattta tgatggatta aagtaaatgt
     2401 ttgtatttcc tgctgacatt aagaaatc
//
LOCUS       DANLXQ010000238         2340 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.245, whole genome shotgun sequence.
ACCESSION   DANLXQ010000238 DANLXQ010000000
VERSION     DANLXQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.245"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..168
                     /locus_tag="PXD94_002578"
     CDS             <1..168
                     /locus_tag="PXD94_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287376.1"
                     /translation="THATEYQTTLGVMKRILEPMLADFEVDPFEEAHYHKALTTLRQY
                     QPKGLGGLVRI"
     gene            404..895
                     /locus_tag="PXD94_002579"
     CDS             404..895
                     /locus_tag="PXD94_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL6287377.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPLVLFADNRQIETTMKDRDKIR
                     HLEIKPDVLHDFTNMPYENESFWHIVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            922..1179
                     /locus_tag="PXD94_002580"
     CDS             922..1179
                     /locus_tag="PXD94_002580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287378.1"
                     /translation="MEQIKLSEKAERALILSQLNENSAMIIDLDDVALILNCGYDYVQ
                     SKISKKPGFPEPVQGINGKSVLFKSGDVIRWVNRQARTRKN"
     gene            complement(1176..2165)
                     /locus_tag="PXD94_002581"
     CDS             complement(1176..2165)
                     /locus_tag="PXD94_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6287379.1"
                     /translation="MASIRKRGSKYQVEISKKGVRKSATFRTKAEAQAWAYEEERKIE
                     IISKGGVVDILFAEALERYQNEVSINKRTWRREIGRINNILKEPIANRYVQDIKRDDI
                     EDWIEARLKRGLKGSSVERDLSILSNVFTYCLKRWGYISENPMIGVIHPNKVPPRKQR
                     YSQEDIDKILEVSKYREDIKRSNARLAAAMLFAIETAMRAGEISGLTWDNVNFEKRIA
                     HLPMTKNGSSRDVPLSSKAIEILKRLETIKKDNSVFQLPQGNGLSARFFELKEKAGLS
                     YLNFHDTRREALSRLAKKVDVMTLAKISGHKDVKILLNTYYAPNMEEVAHLLD"
BASE COUNT          716 a          515 c          425 g          684 t
ORIGIN      
        1 acgcacgcta ccgagtatca aaccacgctt ggcgtaatga agcggatact tgaaccgatg
       61 ttagccgact tcgaggtcga tccgtttgaa gaggctcact accacaaagc cctaactacc
      121 ttaaggcaat accagccgaa aggcttaggc ggcttagtcc gaatttaatt tgcaaaaaaa
      181 tcacacaatc cgaccgcttg tgaaacaaca agcggcggat ttccataccc aaattttgag
      241 gacaagccga tgaaatacgc aaaaatcatt ctatttttaa ccgcctttgc agttgctgct
      301 gaccatctag agttacgcaa cgactgcgat ggcaaaattt gtatggctca acgctaatta
      361 acccaaccgc ttgttccgca agcggttatt ttttggagga aaaatgcaaa caaaaccgat
      421 attagatgcg tgttgtggtt cccgtatgtt tcattttgac aagcaaaatc cactcgtcct
      481 gtttgctgat aatcgacaaa tagaaaccac aatgaaagac cgagacaaaa tccgccattt
      541 agaaatcaaa cctgatgttt tacacgattt cacgaatatg ccatacgaaa acgagagttt
      601 ttggcacatt gtttttgatc cgccacattt agttcaaggt ggagaaaata gctggcttgt
      661 aaagaaatac ggcaaattac cttgcaactg gcgagaagtc ttgaagaaag gctttgacga
      721 atgtatgcga gtgctaaaac caaacggcac gcttattttc aagtggaatg aaacacaaat
      781 ccccgtttca gaaattttaa aagttattga ttgtcagccg atttatggac acaaaagcgg
      841 acgcttaaac caaacacatt ggctggcgtt tgtgaaaatt gagcaatccc tctaaatgag
      901 gggtttattt ttggagagaa tatggaacaa attaaacttt cagaaaaagc ggagagagcc
      961 ttaattcttt ctcagctcaa cgaaaattca gcaatgatta ttgatttaga tgatgttgcc
     1021 ctgattttga attgcggata tgactatgtg caatccaaga tcagtaaaaa gcccggtttc
     1081 ccagagccag tgcaaggcat taacgggaaa tcagttcttt tcaaaagtgg cgatgttatc
     1141 cgttgggtca accgtcaagc acgaacacgc aaaaattaat ccagcaaatg agcgacttct
     1201 tccatatttg gggcgtagta agtgttaagc aaaatcttca catctttatg ccctgaaatt
     1261 ttggctaagg tcatcacatc gacttttttt gccaagcgtg aaagagcctc tctcctcgtg
     1321 tcgtgaaaat tcaaataaga aagcccagcc ttttctttta actcaaagaa tctagcactt
     1381 aaaccattcc cttgtggaag ctgaaaaacg gaattatcct ttttgatggt ttccagtctt
     1441 ttcaagatct cgattgcctt tgatgataac ggaacgtctc ttgatgaacc gttttttgtc
     1501 ataggcaaat gagctattct tttctcaaaa ttcacattat cccacgttaa acccgaaatc
     1561 tcaccggcac gcattgccgt ttcaatcgca aataacatag ccgcagccaa tcgagcatta
     1621 gagcgtttaa tatcttctcg gtatttgctg acttctaaga ttttatcaat atcttcctgg
     1681 gaataacgct gttttcttgg tggcactttg tttgggtgaa taacaccgat catcggattt
     1741 tcactaatat agccccagcg ttttaagcag taggtaaata cattgcttaa aatagacaaa
     1801 tccctctcaa cacttgagcc ttttaaccct ctcttcaatc tagcttcgat ccaatcttca
     1861 atatcatctc gcttaatatc ttgtacataa cgattcgcaa taggctcttt caatatgtta
     1921 ttgattcgac ctatctctcg tcgccacgtc ctcttattaa tcgatacttc attttgatac
     1981 cgctctaacg cttccgcaaa taaaatatca actaccccac ctttcgagat gatctctatt
     2041 ttacgctctt cttcgtaagc ccatgcctgt gcctctgctt ttgtgcggaa ggtagctgat
     2101 tttctcaccc ctttcttcga gatttcgact tgatatttac tgccccgctt gcgaatgctc
     2161 gccatatttt tacctgcctt ttgtcgtaaa tttgtcgtaa tttataccat acaataaccg
     2221 acaataacag ataataaccg attttatgaa atcatcttaa ggcaaagaag agaggaaaaa
     2281 cttaagataa cctattgaaa taactataat aaacgataat aaccgacaaa aaacggaaat
//
LOCUS       DANLXQ010000239         2334 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.246, whole genome shotgun sequence.
ACCESSION   DANLXQ010000239 DANLXQ010000000
VERSION     DANLXQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.246"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..214)
                     /gene="raiA"
                     /locus_tag="PXD94_002582"
     CDS             complement(<1..214)
                     /gene="raiA"
                     /locus_tag="PXD94_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL6287380.1"
                     /translation="MSLNISSKQMDITPAIRTHIEERLAKLEKYHTQLINPHFMLIKQ
                     PNSFQVEATIGTPLGDLVAKAEHEDLY"
     gene            394..1335
                     /locus_tag="PXD94_002583"
     CDS             394..1335
                     /locus_tag="PXD94_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HDL6287381.1"
                     /translation="MSEKKQLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIG
                     LGLGKLFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAW
                     FWSDKRILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPN
                     DNPLLDWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEMIWYAPDHDYGRRNSVFVPFF
                     AVEETCTTAGSYMLLRSAPKSLVIPFSPIRNLDYSGYTVKVSPAVDFSQCQNEVETAI
                     LMNRVVEREIMAALTQYMWLHRRFKTRPNEGDQSLYD"
     gene            1431..2318
                     /gene="dapA"
                     /locus_tag="PXD94_002584"
     CDS             1431..2318
                     /gene="dapA"
                     /locus_tag="PXD94_002584"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL6287382.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     LSESAQPVVLKALQQAGLI"
BASE COUNT          712 a          364 c          505 g          753 t
ORIGIN      
        1 tatataaatc ttcatgttca gctttagcga ctaaatcacc taaaggggtg ccaattgttg
       61 cttcaacttg gaatgagtta ggttgtttta tcagcataaa atgtgggttt atcaattggg
      121 tgtggtattt ttctaactta gctaaacgct cctcaatatg agtacggatt gcaggggtaa
      181 tgtccatttg tttgcttgaa atatttaatg acataacatt ttcctcttgt ttagttaagg
      241 cttttgccgt tgatattcta ataaccaaaa tatgctcatc actccaaaaa ttcaagtgaa
      301 aaatgaaata agtttccaaa aatttgatct acttaacact tttagcagtt tttagctata
      361 atgtaatcat aatctagtat gtgagaataa ttaatgagtg agaaaaaaca gttacctcca
      421 ttccagacac aatttttaca ccctaaatat tggggattgt ggctcggttt aggtgttttt
      481 aagttaattt tatgtttgcc ttatcctatt ttagtaaaaa ttggtctggg actaggtaaa
      541 ttgtttacta aattgggttt tggtaaacgt cgtatgcgta ttgctcgccg taatttagag
      601 ctctgttttc cccattactc tgctgagaaa gtagaggaat tagtcgaaga aaatatgaaa
      661 tcggttggaa tggcgattat tgaaactggt atggcttggt tttggtcgga taaacgtatt
      721 cttaaatggt ctaaaatcga agggctagag catttaacac agcgtgatca ggaaaggggt
      781 attttacttg taggggtgca ttttctgact ttagaactcg gtgcaagaat tattgggcta
      841 catcatcaag gagtaggggt ttatcgaccg aatgataatc cattattaga ttggatacag
      901 tttcgaggac gtgttcgctc aaataaagcg atgttagatc gtaaagatgt gcgtggaatg
      961 attcgagctt taaaaaatgg cgagatgata tggtacgcgc ctgatcacga ttatggaaga
     1021 cgaaatagcg tttttgtacc attttttgca gtggaggaaa cttgtactac tgcgggtagt
     1081 tatatgttac ttcgttctgc cccaaagagt ttagtgatcc cgtttagccc gattcgaaat
     1141 ttggactatt caggctacac agtaaaagtc agtcctgccg ttgattttag tcaatgtcaa
     1201 aatgaagttg aaacggctat tttaatgaat cgggtggtag aacgtgaaat tatggctgcg
     1261 ctaactcaat atatgtggct acatcgccgt tttaaaactc gcccaaatga aggtgatcaa
     1321 agcctatatg attaatttta aataatttca aaaaagtgct tgcatattta agctgaattt
     1381 tgcaatctag caatgttatc aagcataata aaaaaataca ggagatgatt atggcgaccc
     1441 ctttatttca cggtagtatc gtagcattag ttacaccaat gactcatggt gaggtagatt
     1501 atcaggattt aaaaaaatta gttgagtatc atattcaagc aggttctcac gctattgtat
     1561 ctgttggtac aacaggtgaa tctaccacat taagcattga tgaaaatgtt aaggtgatta
     1621 agaaaacggt tgaatttgct gatggacgta ttccaattat tgcaggaact ggttctaatg
     1681 cgaccagtga agctatcact atgactaaat tattagcaaa cagtggtgta gctggttgtt
     1741 taagtgtagt gccttattac aacaagccaa cgcaagaggg gatgtactta cactacaaag
     1801 cgattgcaga aagtacagat ttaccacaaa ttctgtataa tgtgccgggg cgaacaggga
     1861 gtgatttaaa acctgaaaca atcggtcgct tagcgaaaat tgagaatatt gttgctgtga
     1921 aagaagcaac gggtgattta agccgcttac ctttaattaa gcaattagcc ggtgaagatt
     1981 ttattttctt aagtggtgat gatgctaccg gttttgagtc tatgaaatta ggtggtcaag
     2041 gggtgatctc ggtcaccaat aatgttgctg ctgctgatat ggcaaaaatg tgtgagttag
     2101 ctcttgccgg tcaatttact gaagcagaag aaattaataa ccgcttaatg gcgttacacc
     2161 atgacttatt tgtcgaatct aacccgattc cggtgaaatg ggctgcttat aaattaggtt
     2221 taattagtga gcctaattta cgtttaccgc ttaccacact gtctgaatca gcacagcctg
     2281 tagtgttaaa agcattacaa caagcgggct taatttagtt attttttgca aatc
//
LOCUS       DANLXQ010000240         2219 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.247, whole genome shotgun sequence.
ACCESSION   DANLXQ010000240 DANLXQ010000000
VERSION     DANLXQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.247"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..609
                     /locus_tag="PXD94_002585"
     CDS             <1..609
                     /locus_tag="PXD94_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL6287383.1"
                     /translation="AEEAKKIAEAKGIGNKEYKDGLNELEEKNETGKNGEEIRTYGYL
                     YNSHYSVVTAKMKQTLQENGRQMEPTVEVKGLKTENLPTEGKATYKGEAFDSHGNNSN
                     SVVGGELIYNVDFSSRTGSGLVKNVQGGSIELAQGEIKNDSIIASAHQKYNDQAVGNG
                     SYNIQFFGPNAEEIGGKIELNGEGEGSMKQMLGIAGTREEQK"
     gene            658..2052
                     /locus_tag="PXD94_002586"
     CDS             658..2052
                     /locus_tag="PXD94_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6287384.1"
                     /translation="MYKKHFLFLLVSPLVMAETVEFQQDPKEFDGQRQAVKFEQEIAK
                     KDLKLTAYLEEDNSLKGIEQHINQAILRQDWAKLERLLGDYAKQEQFDPLLLDYALAA
                     LAYAKKDYRSAAFYYQQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLR
                     NAVNSALAQIEEEESWKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAK
                     GVRYGVGLSKMRNIGGNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIG
                     VSPFIEQNWLGSSRYNYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYR
                     NQLTLGTQWQAVKNWRFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVG
                     YGKRYFADKHYLFGYKRQDKEYQANVAVWNRSWHWQGIVPKLNFRYQKIDSNIADFYS
                     RSNKEVFMTLEKLF"
BASE COUNT          743 a          377 c          498 g          601 t
ORIGIN      
        1 gctgaagaag caaaaaaaat tgcagaggca aaaggaattg gcaataaaga atataaagat
       61 ggattaaatg agttagaaga aaaaaatgaa acaggtaaaa acggagaaga gattcgaacc
      121 tatggttatc tctataacag ccattattcc gttgtaactg ccaaaatgaa acaaacttta
      181 caggaaaatg gtcgtcaaat ggaacctacg gttgaagtga aagggcttaa aactgagaat
      241 ttaccgacag agggcaaagc aacatataaa ggagaagcct ttgattctca cggtaataac
      301 agcaattcag tagtaggtgg agaattaatc tataatgttg atttctctag tcgtaccgga
      361 tcaggtctag ttaaaaatgt acagggcggt tctatcgagt tagcacaagg cgaaataaaa
      421 aatgatagta ttatagcttc tgctcaccaa aagtataatg atcaggctgt tggaaatggt
      481 agttacaata tccaattctt tggtccaaat gcagaagaaa ttggagggaa aattgagctt
      541 aatggtgagg gcgaaggctc aatgaagcag atgttaggta ttgcaggaac ccgagaagaa
      601 caaaaataac atttaatacc caatgccgtt taatcttaaa cggcattctt ttttactatg
      661 tataaaaaac attttctatt tttacttgtt tcgcctttag taatggcgga aacggttgag
      721 tttcagcaag atcccaaaga gtttgatggg cagcgacaag cggttaaatt tgagcaagaa
      781 attgcaaaaa aggatttaaa attaaccgct tacttggagg aagataattc tcttaaaggt
      841 attgaacagc acattaacca agcgattctt agacaagatt gggcgaagct ggaaaggctg
      901 cttggcgatt atgcaaagca agagcaattt gatccgctac ttttagacta tgctttggcg
      961 gcattggctt atgctaaaaa agattatcgc tctgcggcgt tttattatca gcagttgctg
     1021 aaaaaacagc ctgatttaac ctaccctcgt tttgatttgg ctctgatttt aaaagaggat
     1081 tatcgctaca gggaagcaga gcaagaattt aagcaactca aaaccgatga tttacgcaat
     1141 gcggtgaaca gtgcgttggc tcaaatagag gaggaagaga gctggaagcc ggatttttat
     1201 ttgcaatata ctcaaaccaa caatgtgaat aatgcttctt cgtcgccaac ggtaaatgta
     1261 aacgggcgga tttttattaa agaatcagac agcttgccgc agtcggcaaa aggcgtaaga
     1321 tatggcgttg gcttgagtaa aatgcgaaat atcggcggta atcattttgt gggagtcgat
     1381 cttggctata acggcgttta ttactggaat aaccacgatt atagcgaaca gtcggtctca
     1441 ctaacaccaa aatatagcta cagaacggca acttatcgta tcggggtttc gccctttatt
     1501 gagcagaatt ggctgggtag ctcacggtat aactatcaat ttggtagcag tatcaatgct
     1561 gttaaacttt ttgaacgagg gctgatttcc ggctcattca gccatctgca aaaacgttat
     1621 agtgaacagc atattgccga ccgttacaat ggttatcgca accaactcac gctaggtacg
     1681 caatggcaag cggtcaaaaa ttggcgattt tttgcaaatc ttaacgctag ccgtgaaatt
     1741 acccgtgaga aagctcaatc ttccaataag tggctggcga ccattggcgt aatttaccaa
     1801 accccaagct gggcaagttc ggtaagtgtc ggatatggca agcgttattt tgctgataaa
     1861 cattatctgt ttggctataa acgccaagat aaggaatatc aagcgaatgt ggcggtgtgg
     1921 aatcgaagtt ggcactggca agggattgtg ccgaaattga atttccgtta tcaaaaaatc
     1981 gatagcaata ttgccgattt ctactctcgt agcaataaag aagtctttat gaccttagag
     2041 aaactatttt aaggctgtgt atgaatgtcg gggagaaaaa ttttctcccc gatatttatg
     2101 tgtagaagat cagacttgat ctgacaattc actctaaaaa gtgagagttt cccgtttaga
     2161 atatgagtgt caaaaatcaa tctaaacaaa aaggaaactc tcatgtttta ttctaacaa
//
LOCUS       DANLXQ010000241         2193 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.248, whole genome shotgun sequence.
ACCESSION   DANLXQ010000241 DANLXQ010000000
VERSION     DANLXQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.248"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            83..2014
                     /locus_tag="PXD94_002587"
     CDS             83..2014
                     /locus_tag="PXD94_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL6287385.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDESTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
BASE COUNT          688 a          468 c          477 g          560 t
ORIGIN      
        1 ttcttatttt agttcacaaa gcaatggctt tgctttttta tgctaaaata cgcaaaattt
       61 ttaacaaaat agaccgcttg caatgatctt ttttacagac ttaattttaa aacgagggca
      121 ttccgttctc attgataaca gctccgttac cattcacacc gggcagaaag tcggtttggt
      181 gggaaaaaac ggctgtggca aatcctctct ttttgcctta ctgaaaaatg aattacaacc
      241 tgaaggtggc gatgtatcgt tgccgaaaaa ctgggcgatc tcgtgggtaa atcaagaaac
      301 gcctgcgtta gctatttcag cgttagacta tgtgattcaa ggagatcggg aatataccca
      361 tcttatggct gagctagata tcgcaaatga aaagaatgac ggcaaccgca ttgcgattat
      421 ccatactcaa cttgatacca ttgatgcttg gacaattcaa tcccgtgccg ctaccctgct
      481 taacggcttg gggtttagca ccgaacaact gcaactgccg gtgaaatcct tctccggtgg
      541 ctggcgtatg cggttaaatt tggcacaagc tctgatttgt cgttccgatc tgttattgct
      601 tgacgagcca accaaccact tagacttaga tgcggttatt tggttggagc gttggctgag
      661 taactatcgt ggcactttat tgttaatttc gcacgaccgt gattttctcg atccgattat
      721 cgatcgggtg attcatattg aacatcaaaa gctcaatgaa tacaccggca attacacctc
      781 gtttgagatt caacgtgcga ccaaaatcgc ccagcagaat gccgcttatc agcaacagca
      841 acgtaaagtt gctcatttgc agagttttat cgatcgcttt aaagccaaag cgactaaagc
      901 gaaacaggca caaagccgaa ttaaagcgtt ggaaaaaatg gagcttattg ccccagccta
      961 tgctgattcg ccgtttgaat ttgaatttcg cccaccgctt gcactgccta gcccgttatt
     1021 aatgatggaa aaagtcagtg ccggctatgc ggaaaaaacg gtgttggaat cggtcaaact
     1081 gaatttggtt cccggctctc gtattggttt actcgggaga aacggtgcag ggaaatccac
     1141 gctgattaaa ttattggcgg gcgaacttcc gccccaaaac ggctcaattc aactggcaaa
     1201 aggcgtgcaa ctcggctatt tcgcccaaca tcaggtggat acactacgtt ttgatgaaag
     1261 cccactttgg catttacaaa aactcgcacc ggaaaaaacc gagcaggaat tacgcaatta
     1321 tttaggcggt ttcgatttta aaggcgataa agtggtacaa gctgtgaaat cattctccgg
     1381 cggagaaaag gcccgtttag tgcttgcctt aattgtatgg caacgcccga atttactgct
     1441 attagatgag tcaaccaacc atttagattt agaaatgcga caagccctgg ttgatgcact
     1501 gacctattac gaaggctcgc tagtggtggt ttcgcacgac cgccatttat tgcgtagcac
     1561 cgtgaatgaa ttttatttag tgcacgaccg caaagtggaa aaatttaaag gtgatttaga
     1621 cgattaccaa aaatggctca atgagcaaaa cgccttagcc aacgagccga aatccgtact
     1681 tgcaaaagat tcggcaaatt cgaccgcttg ctctagcacc aatgaaaata gtgcagctca
     1741 acgcaaagag caaaaacgct tagaggcaga gcgccgccaa caaaccgccc cattgcggaa
     1801 aaaaattact caactggaaa aagagttaga aaaagcaacc gaaaaattaa ataaactcga
     1861 agcaatgctt accgaaagcg acatttatga agccgaaaat aaagcaaaac taactgaaac
     1921 acttgcgaaa cagttagagg caaaaaggct ggttgaagaa attgaaatgc aatggttgga
     1981 ggctcaggag gagtttgaga ccatgttacg ctagggttct acaagatagc gcaagcgtcc
     2041 acgcttgtgc catttaaggg aactatacac ctcaaaaaaa caatgatcat taagctagct
     2101 tttttataaa tttaaaaacc tatagattaa ataaataatg atattaatag taaaatcacg
     2161 agtgcaacta cactgacgat aataaaatca ggc
//
LOCUS       DANLXQ010000242         2161 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.249, whole genome shotgun sequence.
ACCESSION   DANLXQ010000242 DANLXQ010000000
VERSION     DANLXQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.249"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..370
                     /locus_tag="PXD94_002588"
     CDS             <1..370
                     /locus_tag="PXD94_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL6287386.1"
                     /translation="NEMDSNTVMLAVLNLIDVVMIANLLVMVIVGGYETFVSRLKVDD
                     HPDQPEWLDHVNATILKVKLSMAIISISSIHLLQTFINASNLDEKQMMWQVVIHLAFI
                     VSALAMAYIDKMLNSGHMKH"
     gene            447..1817
                     /gene="gorA"
                     /locus_tag="PXD94_002589"
     CDS             447..1817
                     /gene="gorA"
                     /locus_tag="PXD94_002589"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL6287387.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEGEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
     gene            1828..>2161
                     /locus_tag="PXD94_002590"
     CDS             1828..>2161
                     /locus_tag="PXD94_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287388.1"
                     /translation="MLRNFMIMLHFIYGFAMKNAFSFFVISLFLTMNSISYASNPIPS
                     NKEKLNGVKKQQDKPKFRISERKVLKNKEKDEEVSNFSMEELRVGAQRPLDDSVIQYF
                     KSQCRYAFM"
BASE COUNT          669 a          311 c          480 g          701 t
ORIGIN      
        1 aaatgaaatg gattcaaata ctgttatgtt agcggtatta aatttaatag atgtggtgat
       61 gattgcaaat cttttggtta tggttattgt tggaggatat gaaacttttg tctctagatt
      121 aaaagtagat gatcaccctg atcagccaga atggttggat cacgttaatg cgactatttt
      181 gaaagtaaaa ctttcaatgg caattatcag tatttcttct attcatttat tacaaacatt
      241 tatcaatgct tcaaatcttg atgaaaagca aatgatgtgg caagtagtta ttcacttagc
      301 ctttattgtt tcagcattag cgatggcgta tattgataaa atgttgaatt cagggcatat
      361 gaaacattag tcttctatgc aaatttaatt tgataaagcg cttaacgaat aagttgcttt
      421 tgtttttata gatcgaggaa tagcttatgg ctaaacatta tgattatatt gctatcggcg
      481 gtggtagtgg tggtattgct tcaattaatc gagcagcaag ctatgggcaa aaatgtgcca
      541 ttattgaagc aaaacattta ggtggcactt gtgtgaacgt gggatgtgta cccaaaaaag
      601 tgatgttcta tggtgcacaa gttgcagaag cgattaacca ttacgcccct gattatggtt
      661 ttgacattac ggtaaataat tttgattatg ccaaattagt tgagagtcgc caggcctata
      721 ttgggcgcat tcatacctca tatggtaatg tgctggcaaa aaataatgtt gatgttttaa
      781 atggttttgc tcgctttaaa gatgctaaaa cattagaagt gagttatgct gatggttccg
      841 ttgaggaggt cacagcagac catattctga ttgcaacagg tggtcgtcca actgtaccca
      901 atgtgaaagg ggctgaatac ggtatcactt ctgatggtgt atttgcacta gatgctttac
      961 cgaagtccgt agcaattgtg ggggcagggt atattgcagt tgaattagct ggagtattta
     1021 atagtttagg agtaaatacc catctttttg ttcgtcaaca tgctccaatg cgtaatcaag
     1081 acccattaat agtggataca ttgcttgaag tattagctca agatgggatc gaattacata
     1141 cacaagcgac tcctcaagaa gtagttaaaa atgcagatgg ttcattgaca ttaaaattgc
     1201 aagatggtcg tgaaacctct gttgaaagct tggtgtgggc tattggtcgt gagccagcga
     1261 cagataagat taatttagag gctgctggtg ttgaaaccaa cgagcgtggt tttatcaaag
     1321 tggataaatt ccaaaatact aatgtgccaa atatctatgc tgttggtgat attattgaag
     1381 gtggtattga attaactcct gttgcagttg cagctggtcg caggctatcg gaacggttgt
     1441 tcaataataa accaaatgaa catttggatt atagcttagt accgacagtc gtttttagcc
     1501 acccaccaat aggtactgtt ggtttaagtg agccaaaagc gattgagcaa tatggggcag
     1561 aaaatgtgaa agtgtataaa tcttcattta ctgcaatgta tactgcggtt acacaacatc
     1621 gtcaaccttg ccgaatgaaa ttagtttgtg ttggggaagg ggaaaaagtg gttgggttac
     1681 atggtattgg ttttggtgtt gatgaaatga ttcaaggttt tgctgttgca attaaaatgg
     1741 gcgcaacgaa agctgatttt gacaacacag tggctattca cccgacagga tctgaagaat
     1801 ttgtaaccat gcgctaaatg taataaaatg ttacgtaatt ttatgattat gctacatttt
     1861 atttatgggt ttgctatgaa aaacgctttt agcttttttg ttatttctct ttttcttacg
     1921 atgaatagta ttagttatgc aagtaatcct attcctagta ataaagaaaa attaaacgga
     1981 gtaaaaaaac agcaagacaa gcctaagttt agaatatctg aacgtaaggt attgaaaaat
     2041 aaagagaaag atgaagaggt tagtaatttt tctatggagg aattacgtgt tggagcacaa
     2101 cgacctcttg atgattctgt gattcaatat tttaaatctc aatgccgata cgcatttatg
     2161 t
//
LOCUS       DANLXQ010000243         2139 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.250, whole genome shotgun sequence.
ACCESSION   DANLXQ010000243 DANLXQ010000000
VERSION     DANLXQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.250"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1877
                     /locus_tag="PXD94_002591"
     CDS             <1..1877
                     /locus_tag="PXD94_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL6287389.1"
                     /translation="DIPHIQCLVRKKDIKLTPEEAVRQLYLMNLHYEYDYPFERMELE
                     YSVVFGREKKRADIVIFDRDNPNAVYIMVELKKPKLKDGKEQLKSYCNATGAPIGVWT
                     NGESISYYNRRDPNYFKDIPDIPNAQQKLSDLLQERWTIQDLIDNDKIAKQRKSLKDL
                     VIEMEDEVLANAGVDVFEELFQLIFTKLYDEMESTRNKSRYLEFRNYGDTESELKAKI
                     QSLFDRAKNKWEGVFAESAKINLSPSHLAICVSSLEEVKLFNSNLDVVDEAFEYLINK
                     SSKGEKGQYFTPRYVIDMCVKMLDPKPEETVIDTAAGSCGFPVHSIFHVWEKQLKARG
                     LERSHLFTAEEKLPEQTDYVKEKVFAIDFDEKAVRVARTLNLIAGDGQTNVLHLNTLD
                     YERWEDFTKEEEWNDVYGEGWKKLRKLRKTKNENRDFQFDVLMANPPFAGDIKETRIL
                     ARYELGKNSKGKQQSKVGRDILFIERNLDFLKDGGRMAIVLPQGRFNNSSDKYIRDFI
                     AERCRILAVVGLHGNVFKPHTGTKTSVLFVQKWHDELCPKVEDYPIFFATMQKSSKDN
                     SGDKIYVKNADGTLKLDNHQHLIVEHDLFNHDNSTQDGIAEAFIEFAKKEKLSFVGKD
                     "
BASE COUNT          741 a          338 c          432 g          628 t
ORIGIN      
        1 gagatattcc tcatattcaa tgtcttgttc gtaaaaagga tattaagctc acacctgaag
       61 aagcagtacg ccaactctat ttaatgaatt tgcattatga atatgattat ccatttgaac
      121 gaatggaatt agaatattct gttgtattcg ggcgtgagaa gaaacgagcg gatattgtca
      181 tctttgatag agataatcct aacgctgtct atattatggt ggagctgaaa aagccgaaat
      241 taaaagatgg taaagaacaa ttaaaatctt attgtaatgc gactggagca cctattggtg
      301 tatggacaaa tggcgaaagt atttcttact ataatcgccg tgatcctaat tattttaaag
      361 atattcccga tattccgaat gctcaacaaa aactttctga tttattacaa gaacgttgga
      421 ctattcaaga tctgatagat aatgacaaaa ttgccaaaca acgtaaatca ttgaaagatc
      481 ttgttattga aatggaagat gaggtactgg cgaatgctgg agttgatgtt tttgaagagt
      541 tatttcaact tatttttact aaactctatg atgaaatgga aagtacacgc aataaaagtc
      601 gttatttaga gtttcgtaac tatggtgata ccgaaagtga attaaaggca aaaattcaat
      661 ctctatttga tcgtgcaaaa aataaatggg aaggcgtatt tgctgaaagt gccaaaatca
      721 acctttcccc ttcgcatttg gcgatttgtg tatcttcatt ggaagaagta aagctgttta
      781 attctaacct tgatgtagtg gatgaagcat ttgagtactt aattaataaa tccagtaaag
      841 gagagaaagg acaatacttt acgcctcgtt acgtcattga tatgtgcgta aaaatgctcg
      901 atccaaaacc tgaagaaaca gtcattgata ccgcagcagg aagctgtggt tttccagttc
      961 attctatttt tcacgtttgg gaaaaacaac ttaaagcgag aggattagaa cgctcacact
     1021 tatttactgc tgaagaaaaa ttgcctgaac aaacagatta tgtgaaagaa aaagtctttg
     1081 cgattgattt cgatgaaaag gcagtacgtg ttgctcgtac cctaaactta atcgcaggag
     1141 atgggcaaac caacgtacta catttaaata ccttagatta tgaacgttgg gaagatttta
     1201 ctaaggaaga agaatggaat gatgtatatg gagaaggctg gaaaaaatta agaaaattac
     1261 gtaaaactaa aaatgaaaat cgagatttcc aatttgatgt gctaatggca aatccgcctt
     1321 ttgccggtga tattaaagaa actcgtatat tagctcgcta tgaattaggc aaaaatagca
     1381 aaggaaaaca gcaatccaaa gtcggacgag atattttatt tattgaacgc aatttagatt
     1441 tcttaaaaga cggtggacga atggcgattg tgctaccaca agggcgtttt aacaattcca
     1501 gcgataaata tattcgtgat tttattgcag agcgttgccg cattttagct gttgtgggct
     1561 tacacggaaa tgtgtttaaa ccacataccg gcacaaaaac ttccgtacta tttgtgcaaa
     1621 aatggcacga tgaactttgc ccgaaagtag aggattaccc gattttcttt gccacaatgc
     1681 agaaatccag caaagataat tcaggcgata aaatctatgt taaaaacgct gatggcacac
     1741 tcaaattaga caaccatcaa cacctaattg tggagcacga tctatttaat cacgataata
     1801 gtacccaaga cggcattgca gaagccttta ttgaatttgc taaaaaagaa aagctctctt
     1861 ttgtgggaaa ggattaagcc catttgaccc tgctaaatat caggcattgt tagatgggct
     1921 agaggtgagc gaggtttatt tgagtaaggt tcgttctgca agttattcgg aacgaatgga
     1981 tagtactttc tttttgaaaa aatacttaaa ccatccgagt tttaattttc cattatcgct
     2041 gaatgaggtt tctctgatta aaagtggtac aacaccaaca gatcgagatg ataacttaaa
     2101 agagggggtt gtcttattaa aaaccaatga tattaggaa
//
LOCUS       DANLXQ010000244         2134 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.251, whole genome shotgun sequence.
ACCESSION   DANLXQ010000244 DANLXQ010000000
VERSION     DANLXQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.251"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..368
                     /locus_tag="PXD94_002592"
     CDS             <1..368
                     /locus_tag="PXD94_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL6287390.1"
                     /translation="FAKVGLQGVDSDFATFIRTVVIIIALIAFLTYSGKWQPLDSLSS
                     KNWLFLILSGLATGLSWSAYFKALQMGNASQVAPIDKFSLVLVTLFAVIFLGERPSGQ
                     DWLGIGLVAGGVLLLAFKR"
     gene            374..1096
                     /locus_tag="PXD94_002593"
     CDS             374..1096
                     /locus_tag="PXD94_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL6287391.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            1106..1525
                     /gene="psiE"
                     /locus_tag="PXD94_002594"
     CDS             1106..1525
                     /gene="psiE"
                     /locus_tag="PXD94_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL6287392.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            1534..>2134
                     /locus_tag="PXD94_002595"
     CDS             1534..>2134
                     /locus_tag="PXD94_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL6287393.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPTLDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTTVQHALSVWDSPGGQLERLHLFLGLIDSTQVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQL"
BASE COUNT          606 a          431 c          460 g          637 t
ORIGIN      
        1 tttttgccaa agttggctta caaggcgtag attctgattt cgccactttt attcgcacgg
       61 tagttattat tattgcactt attgcctttc tcacttacag cggaaaatgg cagccgttag
      121 atagcttgag cagcaaaaac tggctgtttt taatcttatc cggcttggca acaggattgt
      181 cttggtcggc gtatttcaaa gcattgcaaa tgggtaatgc ttcccaagtc gctccaatcg
      241 ataaattcag cttggtgtta gttacccttt ttgcagtaat tttcttaggc gaacgcccaa
      301 gcggacaaga ttggcttggc attggcttag ttgctggtgg cgtactgtta cttgccttca
      361 agcggtgatt tttatgcaaa attttgcaaa tattgaaatt gcgtttattc atcggaaagc
      421 acctctgccg agtgatttct cctaccctga aatgcccggc ggtttaactg aaaaacaggt
      481 gcaaaaatgg caaagccgcc gagtcgcctt ttttctgctg acggaattgt ttaaaaaaca
      541 ccatctggat ttagatttat tagctgatat gcaacgctcc ccaagcggtc gcacttttgt
      601 cgattctgag caaattgact ttaacatcag ccattcaggg gaatggatag cggtcatttt
      661 ttcgtattct tttgcaaaat tagcggtcgg cattgatatt gaacacccac aaaaaatcag
      721 acgttaccac gatttaatct gccattacgg aaacgctgaa gaacaaaatg ttttattagc
      781 caaccactcc cccattctaa gcaacctcgc cgaacgcttt tatttgagct ggtgcttgcg
      841 agaggcgata ttgaaatcgc aaggggtggg gattgtgaaa ctgtccgaag taaaacattt
      901 accgttagaa aaacagatct tttccgccca ttgtccgaaa ggcaagctgc attttgtgag
      961 cgaactgccg ttctatttaa gctacttcta tcaacaacct caaaatatgc tattatctga
     1021 acctttgcta taccattggc aaaacgggca gtttcagcct ataaagtgcc aatcacttgt
     1081 ttatgatgtt aattaaggat aacgaatgcc taaaaaatct ttagaagaac accattccag
     1141 ttttggcaaa attgcttccg aaacctttca gtggattatg aatattgcct tgctagtaac
     1201 aggagcattg ctttcttact cgctgtttgc tgaagcctat tcactctatg aactttcctt
     1261 tagcgacagt gagaaattcc aaattgtaga aaaaatagta attttctttc tctattttga
     1321 gtttcttgcg ttgattgtga aatatttcaa atcgaattat cactttccgt tacgctattt
     1381 cctctatatc gggattactg ccatggtgcg tttgattatt gtcgatcatt ctaatgcgtt
     1441 gcatacacta ttattcgcac tctctatttt ggtgatgatt atagcattat atattgtaca
     1501 caccgatcgt ttacataaaa gctaggaact aatatgaagc taacccaata tcagcacaat
     1561 gatttacaga ttctaaaccg agaaaccgtc tataaagggc attttgagct acaaaaaatc
     1621 actttccgcc ataagctgtt ttccggtgaa atgagcggtg aagtggtgcg tgaactctta
     1681 attaaaggcg aagcggcggc tttaattgcc tatgatccaa ccttagataa ggtagtatta
     1741 attgagcaag tgcggattgg agcttacgat gcaggacaga aaaaatcccc ttggttgctg
     1801 gaattggttg ccggtatggt agataaaggc aacgaagagc cggcagaagt ggcaatgcgt
     1861 gaagccaaag aagaagccgg cttggaagtc acaacagtgc aacacgcctt aagcgtatgg
     1921 gacagtcccg gtggacaact ggaacgcttg cacctgtttc tcggcttgat tgacagcacc
     1981 caagtgaaat ccggcgaggt acacggctta ctggaagaag aaaatgaaga cattttggtt
     2041 cacgttgtaa gccgtgaaca ggcatatcaa tgggtagaag acggcacaat tgacaatgtg
     2101 attgcggtaa ttgggctaca atggttgcaa ttaa
//
LOCUS       DANLXQ010000245         2128 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.252, whole genome shotgun sequence.
ACCESSION   DANLXQ010000245 DANLXQ010000000
VERSION     DANLXQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.252"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1579
                     /locus_tag="PXD94_002596"
     CDS             <1..1579
                     /locus_tag="PXD94_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL6287394.1"
                     /translation="LAQVQQTQKWDFIIIGGGASGLGIAVDAASRGYKTLLLESHDFA
                     KGTSSRSTKLVHGGVRYLANGDVALVREALRERGRLAKNAAHLFKNQSFIIPNYKFKD
                     CVMYRVGLGLYDMLSGNLSLGKTENISKTEAKRRLPTLNDTGLRTGTVYQDGQFDDSR
                     LAVNMAQTVVEHGGTVLNHAKVTSLHKTPQGKIDGVTFYDELNQQEHTVFGTAVINAT
                     GVFMNDILSMDHGSTKKFVVPSQGIHLVLDKSFLPSNDALMIPKTSDGRVLFAVPWHE
                     VLVVGTTDTLIENPSYEPIPLEQEVNFILETAAQYLTKKPTRDDVKAVFAGQRPLAAP
                     EKEGQSTKEVSRSHKVLTDATSGLITITGGKWTTYRQMAEDTVEEALKVHSELGNKPC
                     VTVNLPIHGNIPAAQVNLQDHLYVYGSDIPQLRALMTETPEYAQKIHPRLDYTVAEVI
                     WAVRHEMAQTVEDVLARRVRLLFTDARAAIDSAAKVAAIMAKELGKDEMWQQQQVIQF
                     LDVAKHYLLVDYQPQPI"
     gene            complement(1666..1965)
                     /locus_tag="PXD94_002597"
     CDS             complement(1666..1965)
                     /locus_tag="PXD94_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287395.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLTAFFLLQQKHKRDDS"
     gene            complement(1952..>2128)
                     /locus_tag="PXD94_002598"
     CDS             complement(1952..>2128)
                     /locus_tag="PXD94_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL6287396.1"
                     /translation="KVFFSIAIIMFIAVTICGMMILSALKKQRHFMQSTLTELKLDIA
                     AFKSALTLKQNGEH"
BASE COUNT          678 a          408 c          432 g          610 t
ORIGIN      
        1 gctagctcaa gtacaacaaa cgcaaaaatg ggattttatt attatcggtg gtggcgcaag
       61 tggtttgggg attgcggtag atgcagcttc ccgtggctat aaaaccttat tattagaaag
      121 ccatgacttt gcgaaaggta catcgagccg aagcacaaaa ttagtccacg gtggcgttcg
      181 ctatcttgca aatggcgatg ttgcactcgt gagagaagca ttgcgtgaac gaggtcgttt
      241 agctaaaaat gcagctcatt tatttaaaaa ccaaagcttt attattccaa actacaaatt
      301 caaagactgt gtaatgtatc gtgttggttt aggcttatac gatatgcttt caggcaacct
      361 gagtttagga aagactgaaa acatcagtaa aactgaagcg aaaagacgtt taccaacctt
      421 aaatgatact ggtttacgta caggcactgt ttatcaagat gggcaatttg atgactcacg
      481 tcttgcagtg aatatggcac aaacggtagt agagcacggc ggtacagtac taaatcacgc
      541 caaagtgact tcactgcaca aaacgccaca aggcaaaatt gatggggtga ctttctacga
      601 tgaactcaat caacaagaac atactgtttt cggcactgcg gtgattaacg caaccggcgt
      661 atttatgaat gatattttaa gtatggatca cggtagcacg aaaaaatttg tcgtaccaag
      721 ccaagggatt cacttagtat tagataaatc attcttacca agtaatgatg cgttaatgat
      781 tccaaaaacc tctgacggac gagtgttatt tgctgtacct tggcacgaag tgttagtcgt
      841 agggacaaca gatactctga ttgaaaatcc ttcttatgag ccaattccat tggaacaaga
      901 ggtgaacttt attttagaga cagccgcaca atatctgaca aaaaaaccaa ctcgtgatga
      961 tgtaaaagca gtctttgccg gtcaacgtcc gcttgcggca ccggaaaaag aaggtcaaag
     1021 cactaaagaa gtatctcgta gtcacaaagt tctcactgat gcaaccagcg gtttgattac
     1081 gattacaggt ggtaaatgga caacttatcg tcaaatggca gaagataccg tagaagaagc
     1141 gttaaaagtc cactctgaac taggcaataa accttgtgtg accgtcaact taccgattca
     1201 cggtaatatt ccggcagcac aagtgaatct acaagatcat ctatatgtat atggctcaga
     1261 tattccacag ttacgcgcct taatgactga gaccccggaa tatgcacaaa aaatccatcc
     1321 gagactagat tataccgttg cagaagtgat ctgggcagtt cgccacgaaa tggcacaaac
     1381 ggttgaggat gtattagctc gccgtgttcg tttgctcttc actgatgcga gagcggcgat
     1441 tgatagtgca gcaaaagtgg ctgctattat ggcgaaagaa ttaggtaaag atgaaatgtg
     1501 gcaacaacag caagttatcc agttccttga tgttgcgaag cactatttat tggtagatta
     1561 tcagccacag ccaatctaat acgctaaaag caagcggtta aaatcgataa aaatattgca
     1621 attacgttat ttgcaacttt ttaaaccatt ttaaccgctt gtatattagc tatcatctct
     1681 tttatgtttc tgttgtaata aaaagaatgc tgttaacccc aatacagaaa agctaattcc
     1741 tttcatactc aataatcttt ttaccaaaaa atctaatgca atggttttaa ctgccggtga
     1801 attgaccagt gcaagccctg tttgtttcac ttgtttaaaa ggctgcataa tctcattttt
     1861 ggttttattg gtttccgctt taaatcgcat tctaagtgca ttacctttta gaactagaag
     1921 ttccttttcg gttaatttaa gctcttccat tttaatgttc tccattctgt ttcaaagtta
     1981 aagcactctt aaatgcggca atatctaatt ttagctcggt taatgtactt tgcataaaat
     2041 ggcgttgttt tttcaaggca gaaagtatca tcattccgca aatagtcaca gcaataaaca
     2101 taataatggc aatagaaaag aatacttt
//
LOCUS       DANLXQ010000246         2121 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.253, whole genome shotgun sequence.
ACCESSION   DANLXQ010000246 DANLXQ010000000
VERSION     DANLXQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.253"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1991
                     /locus_tag="PXD94_002599"
     CDS             <1..1991
                     /locus_tag="PXD94_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL6287397.1"
                     /translation="FTAEWTSERAYLLVGVNGTYETTDWLAFHSVKLEKGNVATDWTP
                     APEDVDSAVSAVSAKIDTIQETLANADSALSSRIDAVTASVGSNLAKITQVSNAVAGV
                     DGKLSATHTIKTETIAGGRKAIAGIAFGATADNRNAESSVIVMADKFEVVKNAQDGTP
                     VRLFGVVENKVAINGNLIATGSISGDHINANSVRTAVLTAGAIKTEHLAAGQISADKL
                     AIGLGGNLLVNPIFATPDLSIAPFGWNYWRGEVGDRLNIDTRTIFNRNEQDNYGLKNG
                     GLPNELVFSMRYSTTKETSDTGRIGWLSQDINVVPNKKYIASVWLACHRGQAKLVIEN
                     IEKPGGAYLGRIGNSEVITGYSAHQGEFKNMKRVKVVFTAPTSGCIRFALRLDNIQGK
                     ANPFLFARRPMLEEASDNVTDITQPSPWQNAGVTAIHGGSIVTNTVTAQQIAANTITA
                     NEIATGTIAARNMAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTITGTTI
                     TGNTISGGSINGTTISGTTISGGTIKGARLEGATGKFTGELEVSQLIGGGVIEQVTGK
                     LKYSHRNRIYEGQEHGENSSSPIYRHEYIYKATVTINAAPTRRYIEIIGSESFILEAN
                     QSITKTIEKSLNRNASGSSLDFIVLAYSISTAKYISIS"
BASE COUNT          664 a          397 c          488 g          572 t
ORIGIN      
        1 cctttactgc cgaatggaca agtgagcgag cctatctgct tgttggtgta aatggtactt
       61 atgagaccac agattggtta gcgtttcaca gcgtcaaact tgaaaaaggc aacgtagcca
      121 cagactggac gccagcacct gaagatgtgg acagtgcggt tagtgcagtt tcggctaaaa
      181 ttgatactat tcaggagact ttagcgaatg ccgactctgc attaagctct cgtattgatg
      241 ccgttactgc ttctgttggc tcaaacttgg caaaaattac tcaagtgagt aatgctgtag
      301 ccggtgtgga cggcaaatta tctgctaccc ataccatcaa aaccgaaacc attgccggtg
      361 gacgcaaagc gattgccggt attgcatttg gtgcaacggc agataaccgt aatgcggaat
      421 cttctgtgat tgtgatggca gataaatttg aggtcgtgaa aaacgcccaa gacggcacgc
      481 cagtaagact atttggcgtg gtagaaaata aagtagcgat taatgggaat ttaatcgcaa
      541 caggctcgat ttcaggagac cacatcaatg caaattccgt cagaactgct gtattgactg
      601 caggagcgat taaaacggag catttggcag cagggcaaat atcggcggat aagttagcga
      661 ttgggctagg tgggaatttg ctggttaatc ctatttttgc aacacccgat ttgtcaatag
      721 ctccgtttgg ctggaactac tggcgaggag aagttggtga tcggctaaat attgacaccc
      781 gtactatttt caataggaat gaacaagata attacggact aaaaaatgga ggactaccaa
      841 atgagttagt attttcgatg cggtatagca ctacaaagga aacctctgat actgggcgaa
      901 ttgggtggtt atctcaggat attaatgttg tgcctaataa aaaatatatt gcctccgtat
      961 ggttagcttg tcatcgtggg caagctaaat tagtcattga gaatatcgaa aaacctggtg
     1021 gtgcttattt aggtcggata ggcaacagcg aggtaattac tggctatagt gctcatcaag
     1081 gcgaatttaa aaatatgaaa cgggttaagg ttgtatttac tgcacctact tcaggctgta
     1141 ttcgtttcgc attacgatta gataatattc aaggtaaagc taatccattt ttatttgctc
     1201 gtaggccaat gcttgaggag gctagtgata atgttacaga tatcactcag cctagccctt
     1261 ggcaaaatgc cggtgtaacc gctatccacg gtggatcaat agttactaat accgttactg
     1321 ctcaacaaat tgcagcaaat accattaccg ccaacgaaat cgcaaccggc acgattgcag
     1381 ctcgaaatat ggcagcaaac tcgattaatg caagtcatat tgtggggagt tcgatcactg
     1441 cggataagct aaatgtaaac aatttagcgg caatatcggc aaatcttggt aaggtaactg
     1501 ctggtacaat tacaggtacg acaattactg gtaatacaat tagtggcgga agtattaatg
     1561 gtacaacaat tagcggtaca actatctctg gcggcacaat aaagggtgca agattggagg
     1621 gggctactgg caaatttacc ggcgaacttg aagtatctca attaataggg ggaggtgtta
     1681 tagagcaggt tacaggcaaa ttaaaatatt cacatcgtaa tcgcatttat gaggggcaag
     1741 aacatggaga aaactcctcc tctccaattt atagacacga atatatctat aaagcaactg
     1801 tcaccataaa tgcagcgccg acaaggaggt atattgaaat catcggaagt gagtcattca
     1861 ttttagaagc aaatcaatca atcacaaaaa caatagagaa atcattaaat agaaatgcaa
     1921 gtggtagctc tttagatttt attgttttag cctattccat ttcgacggct aaatatatct
     1981 ctatcagtta gttatcgcct caatgtagta ctgagtgcgg atagccacag cttttttaaa
     2041 tatccaaaac caaccgcttg caggtcaatt caggcgattg tttttatttc aactatcgat
     2101 aaaaggaaaa attatgacaa c
//
LOCUS       DANLXQ010000247         2115 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.254, whole genome shotgun sequence.
ACCESSION   DANLXQ010000247 DANLXQ010000000
VERSION     DANLXQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.254"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            208..888
                     /locus_tag="PXD94_002600"
     CDS             208..888
                     /locus_tag="PXD94_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL6287398.1"
                     /translation="MALNNILGLFAQSPLKPLQKHSNKVTECSELLESFFEATFEHDW
                     EKAAEVRGKIVDLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDI
                     SGRIIGRKLIIPTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNK
                     MILELDQIEDDTDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSR
                     IELMLAKS"
     gene            900..>2115
                     /locus_tag="PXD94_002601"
     CDS             900..>2115
                     /locus_tag="PXD94_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL6287399.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVADTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YSIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVIVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEEKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPV"
BASE COUNT          619 a          379 c          437 g          680 t
ORIGIN      
        1 tctttcgtaa catcacccaa ataaggaata agcgatataa aaaaacaagc ataaatttct
       61 tccatttttt gcaaaaaaat tattaaaaca aaccgcttgt cttaaaaaca taatttaatt
      121 tcgtgatctt tactaataca tcaagtaaaa tacgccggac tttacagtaa gaagtcattg
      181 tcaatattat cactatggag atattttatg gcattgaata acattttagg tttattcgcc
      241 cagtccccac ttaaaccgtt acaaaaacac tcaaataaag tgactgaatg tagcgagtta
      301 ttagaatctt tttttgaagc gacttttgaa catgattggg aaaaagcagc tgaagtgcgc
      361 ggtaaaattg ttgatttaga acgccgcgca gatgccctta aacgtgaaat ccgcttaaaa
      421 ctgcctcgtg gtttatttat gccagttgaa cgcggtgatt tattagaact agtcacacaa
      481 ttagataagt tagcaaacta ttcaagagat atttccggtc gcattattgg tcgtaaatta
      541 atcattccta ccgaaatgca accacttttc aaaaaattcc tttctcgtag tattgatgct
      601 agccgtcagg tacgcaaagt tttggatgaa atggatcagt tattagaaac tggttttaga
      661 gggcgagagc ttgatttcgt caataaaatg attcttgaac ttgaccaaat tgaagatgat
      721 accgaccaat atcaaattac attacgtcat acacttcttg agttagaaaa aaacctgaac
      781 cctattgatg tgatgttttt atacaaatgt attgaaagaa tcagcgttct agctgaccaa
      841 gcccagcgaa ttggttctcg tattgaactt atgctggcga agtcatagga gaaaattaaa
      901 tggaattact caatcaatat ggctcaatac tggtgattat tactgctatt gtagggttta
      961 tgatggcttt cggtattggt gctaatgatg tttcaaatgc catgggaaca tccgtcggct
     1021 ctggaacaat tacgattaaa caagctattt taattgctat ggtatttgaa tttgctggtg
     1081 cttatctggc aggcggtgaa gtcgcagata caattaaaag tggcattatt gatccggcac
     1141 aattcgccaa tagccctgat atcctggtac ttggaatgat ggcatctctc tgtgctgccg
     1201 gaatgtggct aattattgca actaaaatgg gttggccggt ttcagcaact cacacaatca
     1261 ttggtgccgt tattggtttt gcattaatca ctattggttc agatgcaatt caatgggatg
     1321 cattaggtgg aattgttggg agttggtttg tgacccctat tcttgcgggg atcgtagcat
     1381 attcgatctt tattaactca caaaaactga tctttaaccg ctcaaaccca ttcaaacaag
     1441 cgaaaaagta tggcccgatg tacatgggaa ttactatttt tattttagtt attgtaaccg
     1501 tgtcaaaagg cttaaagcac gtaggattac atctagatac agctgaaaca ttgggaattt
     1561 cattcgcgct tgccttactt gcttttatca ttggatattt ttatttccgc agtaaaagct
     1621 ttgcaatgaa agctcgtgca gaagagaaag gcttcgctgg cgttgagaaa atttttagta
     1681 ttttaatgct tattacggcc tgtgctatgg cttttgctca tggctctaat gatgttgcta
     1741 atgctattgg gcctctagct gctgttgaat ctattgtaag aagtggtgga atgattgaag
     1801 gtaaaacgac tttagcccct tggattctcc cactaggcgc cttaggtatg atgattggtt
     1861 tagctattat gggcaagaat gtaatggcta cagtaggtac tggtattacc gaccttactc
     1921 caagccgtgg ctttgctgct caatttgcct gcgccgtcac ggttgtcatt gcatcaggta
     1981 ctggtttacc aatatcaaca acccaaacgc ttgtaggagc agtattaggt gtgggattcg
     2041 cccgtggtat tgctgcactt aacttaggcg ttattcgtaa tattgttata tcatgggtaa
     2101 ttaccttgcc tgtag
//
LOCUS       DANLXQ010000248         2076 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.255, whole genome shotgun sequence.
ACCESSION   DANLXQ010000248 DANLXQ010000000
VERSION     DANLXQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.255"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1172
                     /locus_tag="PXD94_002602"
     CDS             <1..1172
                     /locus_tag="PXD94_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6287400.1"
                     /translation="LKYSPNAKPIADSNGLFILAQKKAKVWIYSYISPKTGKRSTKNR
                     IGSYPQMSLAEARLKRDELNTLVLQGLDPFEHLAKMQREAKEKQESIQSFAYKWADWK
                     LSKGKCKPKTMEKCLQRLENHLFPRFEGYTLEDFTLTDTINRLTDLDRIMPDTLHRIA
                     GNLIEILDYAVLCGRINFNPISVIKKAFSSVSTTHQPAIMPEELAEFMQDLQKSNRTP
                     QIKLLVEWQLLNILRPFEAVAVQWSDIDWETKTLNIPADRMKGGKNPHSVPLTKQAIA
                     ILEEMKKYNGHHKHIFTHRTDRNKPANSQTVNNAIKRLSGGKYKGLLVAHGLRSIAST
                     YLHERFTTETLVIEACLSHTNKDTVMSAYHRKNWLDRRRGIMQEWADFVEQCKSI"
     gene            1642..1848
                     /locus_tag="PXD94_002603"
     CDS             1642..1848
                     /locus_tag="PXD94_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDL6287401.1"
                     /translation="MENTPAKQRFMKIDEVSAVTTLSETQIYRKMKAGDFPQSISIGA
                     NSKVWLESEIQQWIQRKIEQHRNQ"
     gene            1858..>2076
                     /locus_tag="PXD94_002604"
     CDS             1858..>2076
                     /locus_tag="PXD94_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287402.1"
                     /translation="MTLETFNQQKAKFHEQEKAVLAIQTELDKAKNILEALQNEKAEF
                     VARQKTNLANIGTLSADEYVEIKNKNSGL"
BASE COUNT          773 a          434 c          390 g          479 t
ORIGIN      
        1 atctcaaata ttccccaaat gcgaagccta tcgcagacag caacgggcta tttattcttg
       61 cacagaaaaa agcgaaagta tggatttata gctatatcag ccccaaaaca ggcaagcgaa
      121 gcacaaaaaa tagaatcggt agctatccgc aaatgagctt agccgaagca cgcttaaaga
      181 gagacgaact aaataccctt gttttacaag gcttagaccc tttcgagcat ttagccaaaa
      241 tgcaaagaga agctaaggaa aagcaagaat ctattcaatc tttcgcctat aaatgggctg
      301 attggaagct atcaaaggga aaatgcaagc ctaaaacaat ggaaaaatgc ttacagcggt
      361 tagaaaatca tttattccca cgttttgaag gctatacgct ggaagatttt accttaaccg
      421 atacaatcaa ccgcttaacg gacttagatc ggataatgcc cgacactttg cacagaatag
      481 cagggaattt aatagaaatt cttgattatg cggtattgtg tgggagaatt aattttaacc
      541 caatcagcgt cattaaaaag gcgttttcca gcgtaagcac gacacaccag ccagcaataa
      601 tgcccgaaga gttagccgaa tttatgcaag acttgcagaa atcaaataga acgcctcaaa
      661 ttaaattatt agttgaatgg caactcttga atattcttag accgtttgaa gccgtagcgg
      721 tgcaatggag cgatatagat tgggaaacta agacgctcaa cattccggca gatagaatga
      781 aaggcggaaa aaatccgcat tctgtaccgc taacaaaaca agctatcgca atcctagagg
      841 aaatgaagaa atataacgga caccataaac atatttttac acaccgaaca gaccgaaaca
      901 aaccggctaa tagccaaacc gtgaataatg caataaaacg cttaagcggt ggaaaatata
      961 aagggctttt agtggctcac ggtttaagaa gcattgccag cacctattta cacgaacgtt
     1021 ttactactga aacgctagta attgaggctt gtttatccca cacgaacaaa gacaccgtaa
     1081 tgagtgcata ccaccgtaaa aactggctag accgcagacg tggaataatg caggaatggg
     1141 cggattttgt agaacaatgc aagagcattt aaatgacaaa ggcggagcaa tccgcctttc
     1201 tttttgcttg atgaaaaatt ttcatttttt cctatcagct cccagcccgt gccgtctatc
     1261 ccacgcctcg cccgcacact taaagtctcg atttctacgc aaatgcaaaa ttaagcccaa
     1321 acattgcccc gtatagcgtg aaaaactttc atatagccgt tttttattac gcaattttac
     1381 gcaagaaaac gcaaatttac gcaggcagaa acaccacttt taaaaactcg atgacagaac
     1441 gatgaaagaa ccgctgaaaa cttgcaaaaa atcagagaaa aataaccgct tgcaagtttt
     1501 ccaaatcaga attaagcccc tctaatctgt aaaatctcct ttcacagccc taacaaatag
     1561 caaaaaatcc aagatgattt attctttcac acgttagcag gcaatagcac gccaaagcaa
     1621 atttttacgg agtaaaacaa aatggagaac acaccagcaa aacagcgatt tatgaaaata
     1681 gatgaagttt cagccgtaac tacgctttca gaaacgcaga tttatcgcaa aatgaaagcc
     1741 ggcgattttc cccaaagtat cagtattggg gcaaatagca aagtatggct agaaagtgaa
     1801 atccagcaat ggattcaacg caaaattgaa caacaccgca accaataagg gcaaattatg
     1861 acacttgaaa catttaacca acaaaaagct aaattccacg aacaagaaaa agccgtttta
     1921 gctattcaga ccgaattaga caaagcgaaa aatattcttg aggcattgca aaacgaaaaa
     1981 gcggaatttg tcgcacggca aaaaacgaat ttagccaata tcggcacgct ttcagcagat
     2041 gaatatgttg aaatcaaaaa taaaaatagt ggttta
//
LOCUS       DANLXQ010000249         2070 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.256, whole genome shotgun sequence.
ACCESSION   DANLXQ010000249 DANLXQ010000000
VERSION     DANLXQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.256"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(115..1830)
                     /gene="proS"
                     /locus_tag="PXD94_002605"
     CDS             complement(115..1830)
                     /gene="proS"
                     /locus_tag="PXD94_002605"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL6287403.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
BASE COUNT          544 a          520 c          373 g          633 t
ORIGIN      
        1 cacgcattct atttcctata ttctaaaaat aatagagtat tttatacttt agttaaatac
       61 tcgctgcttt atattaaagg gcgtatttta tacgcccaat tttgttattt ataattaagc
      121 ctttacattc gcttttaaga acgcaagtaa gctctctttg gcgatcatct gtttttcacc
      181 gctgcggcgg tttttgtatt cgatttcgcc gttctcgagg tttttctcac caataaccac
      241 catatgcggc acaccgataa gctccatatc cgcgaacatc acgcccgggc gttctttacg
      301 atcatcaaaa atcacctcaa cgccttgagc ttttaagtta gcgtataact cttcagcgaa
      361 tgcctgcact ttttccgatt tgtgcatatt catcggcaca atcgccaccg taaatggcgc
      421 catttcatca caaggccaga taataccacg atcatcataa tgttgttcaa tcgcagctgc
      481 caccacacgg gtaacgccga taccgtaaca acccattgtc atcactagcg gtttaccgtc
      541 ttcaccttgc acagtggctt tcattgcttc tgagtatttt ttgcctaatt ggaaaatatg
      601 ccctacttca atacctcgtt tgatttgaag tgtgccttta ccgtccgggc ttgggtcgcc
      661 ttccaccaca ttgcgtaaat ctgcaatttc cggcacagca acatcacgct cccagttcac
      721 gttaaagtag tgtttgccat caatattcgc accgcagcca aaatctgaca tcaacgccac
      781 tgagcggtca ataatcaccg gaatcggcat attcaccaca cctaacgagc caacaccggc
      841 accgattttg gctttaattt ccgcttcatc ggcaaactca agcggatcag ccaccaacgg
      901 gtgtttttga gctttaattt cgtttaactc gtggtcgcca cgaatcacta acgcaattaa
      961 cggttgctct tcagttgccc ctttcaccac taaggttttc accgtttttt caatggctaa
     1021 accgtgattt tctactaatt cattgatggt tttcgcattt ggcgtatcca ctaattgcat
     1081 ttctgccgtt ggtgcttggc gttcacccac cgcaaccgct tcggctaatt caatattggc
     1141 agcaaaatct gattcggttg aaaataccac atcatcttca ccgcttgagg ctaacacttg
     1201 gaactcgtga gaggctgaac cgccgataga gccggtatct gcctgcaccg cacggaaatc
     1261 taagcccaaa cgggtgaaaa tattgctgta aacctgatac atcacatcat aggtttcttg
     1321 caggctctct tgtgtggtgt ggaaagaata ggcatctttc atcacaaatt cacgcgaacg
     1381 cattaccccg aaacgagggc ggacttcatc acggaatttc gtttgaattt ggtataaatt
     1441 cagcggaagt tgtttgtacg aagaaacttc acggcgaacc aagtcggtaa tcacttcttc
     1501 gtgagtcggg ccaagcacga aatcacgctc gccacgatct ttaaaacgta ataattccgg
     1561 gccgtaatca ttccaacgac cggattccac ccataattcc gccggctgaa ccaccggcat
     1621 taatacctcg atagccccgc ctttgttcat ctcttcacga ataatatttt ccactttttt
     1681 caacactttg atcccggtcg gcaaccagtt gtataaacct gacgccatcg ggcgaatcat
     1741 tccggcacgc aacattaatt gatggctaac tacttgtgca tcattcggca tttctttaag
     1801 ggttgaaaat aaatattgac ttgtacgcat tgctattcct attcttttta aactttgaaa
     1861 cctgaaaaaa tctgcttatt ttaaaacaaa gcggttaaat tttcaggaaa ttttgcaaaa
     1921 tcctataaaa aaataaccgc ttgtaagcgg ttattttgtt ttaactaagg gtattttctt
     1981 aaactgtgta tgcttaattt tctcgttcaa tttgctcaat aatctcttgc cattcggcat
     2041 ttgcttgttc aagagtaaaa atctgacctt
//
LOCUS       DANLXQ010000250         1962 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.257, whole genome shotgun sequence.
ACCESSION   DANLXQ010000250 DANLXQ010000000
VERSION     DANLXQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.257"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..1783)
                     /locus_tag="PXD94_002606"
     rRNA            complement(<1..1783)
                     /locus_tag="PXD94_002606"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          439 a          563 c          361 g          599 t
ORIGIN      
        1 ttaataaaca gttgcagcca gctggtatct tcgaccggtt caaccttcat ccgcaaggga
       61 ctacaatcta cgccggcgca ccttctcccg aagttacggt gctattttgc ctagttcctt
      121 cacccgagtt ctctcaagcg cctgagtatt ctctacctga ccacctgtgt cggtttatag
      181 tacggtttat aataacctga agcttagtgg cttttcctgg aagcgtggta tcgattactt
      241 catctccgta gagactcgtc atcacttctc ggtgttaagg aagaccggat ttgcctaatc
      301 ttcccaccta ccggcttaaa cagacatcca acagtctgct aacctaacct tctccgtccc
      361 cacatcgcag ttattacaag tacgggaata ttaacccgtt tcccatcgac tacgcttttc
      421 agcctcgcct taggggccga ctcaccctgc cccgattaac gttggacagg aacccttggt
      481 cttccggcga acgagttttt cactcgtttt atcgttactt atgtcagcat tcgcacttgt
      541 gatatctcca gcagacttct caatccacct tcatcgactt acacaacgct cccctaccca
      601 acagactttc gtctgatgcc gcagcttcgg tgctatattt gagccccgtt acatcttccg
      661 cgcaggccga ctcgactagt gagctattac gctttcttta aatggtggct gcttctaagc
      721 caacatccta gctgtctaag ccttcccact tcgtttccca cttaatatag actttgggac
      781 cttagctggc ggtctgggtt gtttccctct ccacgatgga cgttagcacc caccgtgtgt
      841 ctcctgagta tcactcttcg gtattcgcag tttgcatcgg gttggtaatc cgggatggac
      901 ccctagccga aacagtgctc tacccccgaa ggtgtccgct caaggctcta cctaaataga
      961 tttcggggag aaccagctat ctcccggttt gattggcctt tcacccccag ccacaagtca
     1021 tccgctaatt tttcaacatt agtcggttcg gtcctccagt tagtgttacc caaccttcaa
     1081 cctgcccatg gctagatcac cgggtttcgg gtctatacct tgcaactaca cgcccagtta
     1141 agactcggtt tccctacggc tcccttattc agttaacctt gctacaaaat ataagtcgct
     1201 gacccattat acaaaaggta cgcagtcaca agattactct tgctcccact gcttgtacgc
     1261 acaaggtttc aggttctatt tcactcccct cgccggggtt cttttcacct ttccttcaca
     1321 gtactggttc actatcggtc aatcaggagt atttagcctt ggaggatggt ccccccatct
     1381 tcaaacagga tatcacgtgt cccgccctac ttgttgttag cctagtacca caatggagtt
     1441 ttaaggtacg ggattatcac cctctacgat tgtccttccc agaacattcc cctaacttta
     1501 ctgctatcac taactggctc tttcgcgttc gctcgccgct actaacgaaa tctcggttga
     1561 tttcttttcc tcggggtact tagatgtttc agttctcccg gtttgcctca cacagctatg
     1621 tattcactgg gtgatagtag gttcttcacc tactgggttt ccccattcgg acatcttgga
     1681 ttaaacgcct cttatcgact catccaagct tttcgcagat tagcacgtcc ttcttcgcct
     1741 ctgattgcca aggcatccac cttgtgcgct tagtcactta actatacaac ctcaaatgtt
     1801 ttctaaaact tctcaaaaat aaaagtaaaa aaacaatgaa gtcagtttta caactaaaca
     1861 cttgactgct tttgttcagt caagattttt tctactcaga ctttcaattt attcgttatc
     1921 acttatgtat aattacttat gcgtaacgaa cttgaaatat ct
//
LOCUS       DANLXQ010000251         1949 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.258, whole genome shotgun sequence.
ACCESSION   DANLXQ010000251 DANLXQ010000000
VERSION     DANLXQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.258"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            51..737
                     /locus_tag="PXD94_002607"
     CDS             51..737
                     /locus_tag="PXD94_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL6287404.1"
                     /translation="MFGLFASILTGARGGWLAFIPICFFILFLNLRQLNKKAIFLILL
                     ALISSLSFFISKPEFGIQKRYNAAKNDIIVFLEKGNRHSSLGARFDMWENALIGIREK
                     PLLGHGSTGYETFKKKQIETKQMAKTTLRFNSLLHNQYLEAFVKRGVVGFMALMAILI
                     IPFVIFAKRLKAKNREVKCMAILGIVHIISHSFFFLSQSFLAHNSGSLFYFFVLILLY
                     HLVKQNEEII"
     gene            complement(839..1846)
                     /gene="rfbB"
                     /locus_tag="PXD94_002608"
     CDS             complement(839..1846)
                     /gene="rfbB"
                     /locus_tag="PXD94_002608"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL6287405.1"
                     /translation="MIILITGGAGFIGSALVRYLIEHTPHTVINVDKLTYAGNLASLE
                     SVAHHERYHFEKADICASGQISEIFGKYQPDVVMHLAAESHVDRSISGAADFIQTNIV
                     GTYTLLEAARAYWDTLPEPRKSAFRFLHISTDEVYGDLAKSEELFTEKTPYNPSSPYS
                     ASKAASDHLARAWHRTYGLPTVITNCSNNYGAYQYPEKLIPLMILNALEGKSLPVYGN
                     GLQVRDWLFVEDHIRALYLVLQKGQIGETYNIGGHNEKTNIEVVRSICSILQEMVPNN
                     PLVVGQYADLITYVQDRPGHDFRYAIDTSKIEKELDWKPKETFESGLRKTVEWYLANR
                     N"
BASE COUNT          590 a          377 c          371 g          611 t
ORIGIN      
        1 ggctttcaaa aaaaagaatt aaaactggca ttgcctacct ggtttttgcc atgtttgggt
       61 tatttgccag tattctaaca ggagcaagag gcggctggct tgcattcatt cccatttgct
      121 tttttatcct atttttaaat ttaagacagc taaataagaa agctattttc ctgattctat
      181 tagcacttat atcttcactc tcatttttta tctccaaacc agaatttggc atacaaaaac
      241 gatataatgc agctaagaat gatattatcg tatttttaga aaaaggaaat agacattcat
      301 ctctgggagc tcgttttgat atgtgggaaa atgcgcttat cggaatccgt gaaaaaccgc
      361 tattaggaca cggtagcaca ggctatgaaa cattcaaaaa gaaacagatt gaaacaaaac
      421 aaatggcaaa aacaacgctt aggtttaata gcttattaca caaccaatac ctagaagcct
      481 ttgtgaaaag aggtgtagtt ggttttatgg cgctaatggc tattttaatt attccatttg
      541 ttatttttgc caaacgatta aaggcaaaaa atagagaggt aaaatgtatg gcgatattag
      601 gcattgtaca tattatttcg cattcattct ttttcctaag ccaatcattt ttagcccata
      661 attcaggctc attattctat ttctttgtgt taattttact ctaccattta gtcaaacaga
      721 acgaagagat aatatgataa aacaatagaa gaaagttggg aatataaccg aggtggtaac
      781 ttcatcttct accacaggat aaatgtcggg atatttcctc tttttctgct tcttactact
      841 aattccgatt agccaaatac cattccactg ttttacgcaa gccactttca aaagtttctt
      901 tcggtttcca gtcgagttcc ttttcgattt ttgaagtatc aattgcatag cggaaatcgt
      961 ggccagggcg atcttgcaca taagtaatta aatcagcata ttgtcctaca acaagcgggt
     1021 tatttggcac catttcttgc aaaatagaac agatagaacg aaccacttcg atattggttt
     1081 tttcattatg cccgccaata ttgtaggttt ctccgatttg ccctttttgc aacaccaaat
     1141 agagggcacg aatatgatct tccacaaaca gccaatctct gacctgcaag ccgttgccgt
     1201 aaacaggtag ggatttacct tctaaggcat ttaaaatcat taacggaata agtttttcgg
     1261 gatattggta tgccccataa ttattggaac agttggtgat gacagtcggt aaaccgtagg
     1321 tgcgatgcca agcccgagcc agatgatcgc tggcagcttt agatgccgaa taggggctac
     1381 ttggattata aggggttttc tcggtaaata attcctcgct tttggctaaa tcaccataga
     1441 cctcgtcggt cgagatatgt agaaagcgaa acgccgattt ccttggctca ggtaacgtgt
     1501 cccaataggc tctcgctgcc tctaataaag tataagtgcc gacaatattg gtttgaataa
     1561 aatctgctgc cccagaaata gagcgatcaa catggctttc cgctgctaag tgcattacca
     1621 catcaggctg atatttgcca aaaatctcag aaatttgacc gcttgcacaa atatctgctt
     1681 tttcaaaatg gtagcgttcg tgatgtgcga cactctccaa tgaggctaaa tttcccgcat
     1741 aggtgagctt atccacattg ataactgtgt gtggagtatg ttcaatcaga tagcgaacta
     1801 atgcagatcc gataaaaccg gctccgccag tgatgaggat gatcataaag gtgaatatga
     1861 aatttgattc acgaaatttc gtttgtattt aatatggctt tgaatattct tttcagcgtc
     1921 ttttttatcc caaatttgca attcccaag
//
LOCUS       DANLXQ010000252         1949 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.259, whole genome shotgun sequence.
ACCESSION   DANLXQ010000252 DANLXQ010000000
VERSION     DANLXQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.259"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1642
                     /locus_tag="PXD94_002609"
     CDS             <1..1642
                     /locus_tag="PXD94_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL6287406.1"
                     /translation="DLNADTSQAFLNELDQTLWTAADKLRKNLDAANYKHIVLGFIFL
                     KYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDYYAAENIFW
                     VPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLKGVLQRIAG
                     FGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGG
                     QYFTPKSIVTLIVEMLEPYSGRIYDPAMGSGGFFVQADRFIQAHAGNRNAISVYGQES
                     NSTTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQKEWWNESL
                     ANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGEGDIRKNIV
                     QADLVEAMIALPNQLFTNTQIPACIWIINKAKARKGEVLFINATQIGYLKDRVLRDFT
                     ADDIAKISDTYHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEENDGV
                     PFAEKMQELTALLNEQFKQGRELEQQIAENLKGLGYGI"
     gene            1632..>1949
                     /locus_tag="PXD94_002610"
     CDS             1632..>1949
                     /locus_tag="PXD94_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6287407.1"
                     /translation="MAFNQYVFSDIVELISEKIKIKDLKKENYISTDNMLPNFGGITL
                     AENLPNSASCNRFAKKDILFSNIRTYFKKVWLAEFSGGCSPDVLVMRSKNTDILLNEY
                     LFLL"
BASE COUNT          594 a          479 c          402 g          474 t
ORIGIN      
        1 tgatctcaac gctgacacaa gtcaagcctt tttaaacgaa ctcgaccaaa ccctctggac
       61 tgccgccgac aaactgcgta aaaacctcga tgccgccaac tacaaacaca tcgttcttgg
      121 ctttatcttc ctaaaataca tctccgacag ctttaccgat ttccaagcca agctaaaaac
      181 ccagcttacc acccccgaaa gcgaactcta tcttgaccct gcactatttg acgaacaaga
      241 atttagccaa attcttgccg aagagttgga acagagagat tactacgccg ctgaaaacat
      301 cttttgggtg ccggagcaag cccgctggga caacatcaaa tcattaagca aactcaatct
      361 tggcgatgaa ttgccttggg gagacaaatt taaaggtgtc agccgcttga ttgatgatgc
      421 ctttgaagcc atcgaacggg aaaaccccaa actcaaaggc gtactccaac gcattgccgg
      481 ctttggcgtg cctgatgaaa tgctcacagg cttaattgac ctgttctcac gcaccaattt
      541 cacccagcct atgcacaatg gcgaacctgt gcatctgcaa gccaaagaca ttttagggca
      601 cgtctatgaa tactttcttg ggcaatttgc ccttgccgaa ggcaaaaaag gcggtcaata
      661 cttcacgcca aaatccatcg ttaccctgat tgttgaaatg ctcgaaccct attcagggcg
      721 gatttacgac ccagctatgg gcagcggcgg cttttttgtg caagctgacc gctttattca
      781 ggctcacgca ggcaaccgca acgccatttc cgtttatggg caagaatcca actccaccac
      841 tcgcaaactg gcggtgatga atatggcgat tcgtggtatt ccctttgact ttggcgacaa
      901 gcccgaagat accctactaa accctttgca catcgacaaa aaaatggatg ttgtgatggc
      961 aaatccgccc tttaaccaaa aagagtggtg gaatgaaagc ctagcaaacg atccacgctg
     1021 ggcatacggc acaccgccgc aaggcaacgc caactttgcg tggttgcaac atatgattta
     1081 ccacctctcc cccaaaggca aaatggcact cctgctcgcc aacggctcaa tgagcagcca
     1141 aacttcaggc gaaggcgaca ttcgcaaaaa catcgtgcaa gctgaccttg tcgaagcgat
     1201 gattgccctg cccaatcagc tattcaccaa cacccaaatc cctgcctgca tttggattat
     1261 caataaagcc aaagccagaa aaggtgaagt gctgtttatc aacgccaccc aaataggcta
     1321 cctgaaggac cgcgtcttgc gtgattttac cgctgatgac atcgccaaaa tcagcgacac
     1381 ctaccacaac tggcaaaaac agaacggcta cgaaaatatc cctgcgtttt gttattgtgc
     1441 cacgctggac gaaatcgcca aaaacgattt tgtgctgacc gcagggcgat atgtcggtgc
     1501 ggtacaagaa gaaaatgacg gcgtgccgtt tgcagaaaaa atgcaggaat tgaccgcttt
     1561 attgaatgaa caatttaaac aagggcggga attggaacag caaatcgcag agaatttaaa
     1621 ggggttggga tatggcattt aatcagtatg tattttcaga tattgttgaa ttaatatccg
     1681 aaaaaataaa aatcaaagac ttaaaaaaag aaaactatat ttcgacagat aatatgctgc
     1741 ctaattttgg tggaataaca cttgctgaaa accttccaaa ttcagcttct tgcaataggt
     1801 ttgccaaaaa agacattttg ttttccaata taagaaccta ctttaaaaaa gtttggcttg
     1861 ctgaattttc aggtggttgt tctcctgatg ttctggtaat gcgcagtaaa aacacagata
     1921 ttttattaaa tgagtattta tttttactt
//
LOCUS       DANLXQ010000253         1940 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.260, whole genome shotgun sequence.
ACCESSION   DANLXQ010000253 DANLXQ010000000
VERSION     DANLXQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.260"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(34..324)
                     /locus_tag="PXD94_002611"
     CDS             complement(34..324)
                     /locus_tag="PXD94_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287408.1"
                     /translation="MNNLSEFKLRYINNPYSNKDTFFIFDELSILLKSLTSDELKKIG
                     TAKNLADRQLNKQRKRCKRYVSEAYRLVEDNKEPYSKFFNHDGITAYNIKAF"
     gene            complement(374..1138)
                     /locus_tag="PXD94_002612"
     CDS             complement(374..1138)
                     /locus_tag="PXD94_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6287409.1"
                     /translation="MPNITPKLVINALEPAKEKGIGDTLKRAIRLLNEVMNFAVNADV
                     IEFNKCQNVGSNFSVAKSEHNPTIHPKELPQFLAALRDYNLSFQTKMLIKWQLLTMTR
                     SREASETLWSEIDLEAKTWTISAERMKMKRPHVIPLSRQAVEILERMRKITGNSPFVF
                     QSEMKPRQPMNSQTANRAIGLLGYAGTLTAHGMRSIASTYLNEQLVNYDVVEACLAHV
                     IKDQTRKAYNRSDYLDQRVDVMQQWADYVESCSTGI"
     gene            complement(1107..1586)
                     /locus_tag="PXD94_002613"
     CDS             complement(1107..1586)
                     /locus_tag="PXD94_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL6287410.1"
                     /translation="MARVTTELNNTQIKNAKAQTKDYKLMDGKGLFLLVKKNGSKLWR
                     FRYKKPITGKESDLGIGSYPETTLAQARTIREEYRALLAENIDPLEHRQRLEAEALAE
                     IENTFYKVCLKWRDKCESRVKNGELQALTLKKNWRILEKYLLPCRLLYSKHHAKTSY"
BASE COUNT          539 a          373 c          362 g          666 t
ORIGIN      
        1 tcaggacgat ataaaaaagg ctgtctaaat agattagaaa gccttgatat tgtatgcggt
       61 tatcccatcg tggttgaaaa acttagagta aggttcttta ttatcttcaa caagacgata
      121 agcctctgat acataccttt tacatctttt tctttgctta ttaagttgtc tatctgccag
      181 atttttagct gttcctattt tctttaattc atcggaagtc agtgatttta atagtatgct
      241 taattcatca aatataaaaa atgtatcttt attagaataa gggttattga tatatcgtaa
      301 tttaaactca gaaaggttat tcatatttgt acacctctaa ttaaacaagc ggtcaaattc
      361 cgccaaaaat ttactaaatc cccgttgagc agctttctac ataatccgcc cattgctgca
      421 ttacatctac acgctgatct aaataatcag aacggttata agcctttcgg gtttggtctt
      481 tgataacgtg agctaaacaa gcctctacta catcataatt gactaactgc tcattcaaat
      541 aggttgaggc aatagaccgc ataccgtgag ccgttagagt gccggcatag cctaataagc
      601 ctatggctct gttggcggtt tggctgttca tcggttggcg tggtttcatt tcgctttgaa
      661 aaacaaatgg gctattccct gtgatttttc gcattctttc taaaatttcg accgcttgcc
      721 gtgaaagagg gataacgtga gggcgtttca tcttcattct ttcggctgaa atggtccacg
      781 ttttcgcctc taaatcaatt tcactccaca acgtttcgct tgcctctcgt gagcgtgtca
      841 ttgttaatag ttgccattta atcaacatct tagtttgaaa tgataggttg taatcacgca
      901 atgcagctaa aaattgaggt agctcttttg ggtgtatcgt tgggttatgt tcagatttcg
      961 ctacgctaaa atttgagcct acattttggc atttgttaaa ctcgataacg tccgcattta
     1021 ccgcaaaatt catcacttca ttaagtagcc taattgctcg tttaagggtg tcgcctatgc
     1081 ctttttcttt agcaggctct aaagcattaa taactagttt tggcgtgatg tttggaatag
     1141 agtagtcggc aaggcaaaag atacttttca aggattcgcc aattcttctt cagcgttaag
     1201 gcttgaagtt cgccattttt tacacggctt tcgcatttat cacgccattt caagcagact
     1261 ttataaaagg tgttttctat ttcggctaac gcttcagcct ctaatctttg gcggtgttcg
     1321 agtgggtcga tattttccgc tagtaatgcc ctatattctt ctctaatcgt tcttgcttgg
     1381 gctagggtag tttcggggta actgcctatg cctaaatcgc tctctttgcc ggtaatcggc
     1441 tttttatacc taaaccgcca taacttcgag ccgtttttct taaccagcaa aaacagcccc
     1501 ttgccgtcca ttagcttgta gtctttggtt tgagcctttg cgtttttgat ttgagtgtta
     1561 tttaattctg tggtaactct agccataaaa atttacctaa aatcgcattg tgctttatca
     1621 gaatcataag ggatttaagg gtatgggttt catacccttt tacgttatac cctcaaatat
     1681 acccttaaaa taaggggatg taaacgcaag aacacgaaag aatacgaatg aagaaataat
     1741 ttaaaattgc tctaaagcca gtatcatcaa tggttttgcg taagaatgcg aatgaagaaa
     1801 taagagtgga aaatttaagt tggtcccagc ctcctctgaa ctcgcttata acttattgat
     1861 ttataaagag ataaaataaa ctctacaaaa atagggatca ttttagggat catttacata
     1921 aaagctattt attttcgttt
//
LOCUS       DANLXQ010000254         1926 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.261, whole genome shotgun sequence.
ACCESSION   DANLXQ010000254 DANLXQ010000000
VERSION     DANLXQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.261"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            30..341
                     /locus_tag="PXD94_002614"
     CDS             30..341
                     /locus_tag="PXD94_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6287411.1"
                     /translation="MVIIMLSKTKSPTLFPKYQNALLQLGENIRLASKRRMFTQTLLA
                     QRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQDFTDVGKDDELGRKLQDIELLR
                     K"
     gene            341..1582
                     /locus_tag="PXD94_002615"
     CDS             341..1582
                     /locus_tag="PXD94_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL6287412.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEINNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
BASE COUNT          649 a          335 c          383 g          559 t
ORIGIN      
        1 ggacattata atttccaaaa aacataaaaa tggtaataat aatgctcagt aaaacaaaat
       61 caccaactct gtttcctaag taccaaaatg ccttgcttca acttggagaa aatattaggc
      121 tagcctcaaa aaggagaatg tttactcaaa cattattggc tcaaagaact gggcttagcc
      181 gtgtaacagt gagaaaaatt atgaatggtg atccaactgt tgcgattgga tactacctaa
      241 tggttttagg tgttctcggt ctagaacaag atttcactga tgttggaaag gatgatgagt
      301 taggacgtaa attgcaagat attgaattat taaggaaata atgatgaata aacgaattta
      361 tgtgtttgca gattgggaaa ctttttcaca gcctgaatta gttggggtgc tttcttctga
      421 tgtagtgaga ggcaaagaaa atttccgttt tagctacgat aacgagtggt taagttcacc
      481 ctatgtacag cagattgatc ctaacttata cctttaccct ggagagcaat acagtacaga
      541 tacacaaaat tttcatatct ttttagattc ttgccctgat agatggggta gattgttaat
      601 gaaacgtcgg gaggcagctc tagctagtca agagaatcgt aaacctaagt tactaataga
      661 aagtgattac cttttaggcg tacacgatat gaatagaatg ggggctttac gttttaaaac
      721 tgaattgaat ggtccatttt tggacaataa tactcgtctt gcagcaccac cactgtcttc
      781 cttaagagaa ttagaatttg cagcaataca acttgaaagc agtgataatg tggacgatcc
      841 ggattattta aaatggcttc atatgttgat atcaccaggg tcgtcactag gcggagctag
      901 accaaaagct tcggtaatag atgaactaaa tcaactatgg attgccaaat ttccaagtcg
      961 gtacgatgat tatgatattg ctctttggga atatttagct tatcgactgg ctattcaagc
     1021 caaaattgaa atggctactt gtagaattga acgctttaac caagaacacc atactttttt
     1081 gacacaaaga tttgatcgac attgtggaaa acgtcgccat tttacgtcag cattaaccca
     1141 gctgggttat tatgacggag actatgaggc aagctacctt gagattgctc aatttttaac
     1201 agaaagtggt tcaaatactc gaattgactt ggctcaacta tggagaagaa ttgtattcaa
     1261 tattgctatt tcgaatactg atgatcattt gcgtaaccac ggatttatct acgctcaaga
     1321 ggggtggata ttatctcccg catacgattt aaacccagta ccactatctc aaggattaca
     1381 tttaaacata acagacagcg ataaccgatt ggattatcag ctcgcctttg atgttgctga
     1441 cttttttcaa ttatctaagc aacaagccgt acagatttat gatgaagtac tgaaagaaat
     1501 taataattgg caaaatgtcg caaaagaaat tggtattcca cgccaagagc aagagcagat
     1561 gaaatttgca tttaatgtat agtaattgac ttgtggaacc aatcatcgta gtctatgttc
     1621 taaaaaagta gcgttacctt aataacgcaa tgagttgttg gctcattgtc aatctgatat
     1681 taatctagga tgtaaaggcc tagcggtggc aggcaccaga aagacctgat actaatctag
     1741 ggtataaaga cctagcggtg gcagacacca gaacaaacca acatcttata aaaccattag
     1801 taacacttga cttttttctc aaccatatcc gcaaggaagc gtagccttaa aaacgcaaat
     1861 gtaggaaact aatctaggca tatataatgc ctagcagatg gcagacatca gaaaaactaa
     1921 cattta
//
LOCUS       DANLXQ010000255         1919 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.262, whole genome shotgun sequence.
ACCESSION   DANLXQ010000255 DANLXQ010000000
VERSION     DANLXQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.262"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..626
                     /locus_tag="PXD94_002616"
     CDS             <1..626
                     /locus_tag="PXD94_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL6287413.1"
                     /translation="NQAEDKANYFEISDLDYIKAEAQGELVAVVHSHPEPNGKPVLST
                     LDRKMQVQTGLDWWLVHNQKIHKFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMD
                     EFERQDDWWHTGQNLYLDNIQGQGFERVETPQIGDVILMQVGADVPNHAAIYIGDQMV
                     VHHSPNRLSKRDLYDGYWLRHTHSIWRHKLAEKLDFDGILNDIAVNN"
     gene            895..1524
                     /locus_tag="PXD94_002617"
     CDS             895..1524
                     /locus_tag="PXD94_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL6287414.1"
                     /translation="MITVKFYGHLKTFGTDFKILVKDIAEAIRALCSQLKGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVVKGAKSGGIFQAVLGVALIGAA
                     FALGPIGLGLVGSATAMTVGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            1534..>1919
                     /locus_tag="PXD94_002618"
     CDS             1534..>1919
                     /locus_tag="PXD94_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287415.1"
                     /translation="MRIYGAKGGGGSSHTPIEAPETGRSKQIVNIVELLCEGEIEGLV
                     DGFKSIYLDGTQIQNDDGTYNFNNVSGQLNVGTQDQDVLDGYDSSQNEVNVGVEIKKK
                     NGAIVRTVTDERINRLRLTLGVRSLF"
BASE COUNT          587 a          372 c          460 g          500 t
ORIGIN      
        1 aaaaccaagc ggaagacaaa gccaattact ttgaaatctc tgatcttgat tacatcaaag
       61 cagaagcaca aggcgagctg gtggcggtag ttcattcaca ccctgagcca aacggcaagc
      121 cggtactctc tacccttgat cgcaaaatgc aagtacagac cggtttagat tggtggctgg
      181 tacataatca aaaaatccat aaattcagga atgtgccgca tttaatcggg cgtgagttta
      241 aacacggtac aatggattgt tacacgctct accgtgatgc ctatatgctt gccggttacg
      301 agatggacga gttcgagcga caagatgact ggtggcatac cgggcaaaat ctctatttgg
      361 ataatattca agggcaaggc tttgagcgag tggaaacccc acaaatcggc gatgtgattt
      421 taatgcaagt aggtgctgat gtgccgaacc acgctgccat ttacattggc gatcagatgg
      481 tagtccatca tagccccaac cgattatcta agcgtgattt atacgacggc tactggttac
      541 gccataccca cagtatttgg cggcataaac tggcggagaa gttagatttt gatggcatat
      601 tgaatgatat tgctgtaaat aactaacaaa aaatgaccgc ttgtaacaaa aacaaacccc
      661 gaagcgtttg cagcacttcg gggtttttca tatccactta cctaactaag aggacataaa
      721 ttaaatgaat gataaacgat ttacattcaa atttctagga gttcttatgg aagcaataaa
      781 tattacccca aaagaaatca gaaaaacaat gtggacagcc gcaataattc tctttttgtt
      841 tgcgttcatc tggaaagccc ctgaatttat cactgctatt cgttggtggt aactatgatt
      901 acagttaaat tttacggaca ccttaaaaca ttcggcacag attttaaaat tttagttaaa
      961 gacatcgctg aggcaattcg tgctctgtgt tcacagctta aaggattgcg tgaagcgttg
     1021 cgagacggtg tttataaagt ccgtatcggt aagcagtatt tagacccatc agcccttgaa
     1081 aaagggcttt tttattgcct gaagaaaggg caaaccattc attttacccc tgttgtcaaa
     1141 ggagcgaaaa gcggtggtat ttttcaagcg gtgttaggtg tggcgttaat tggggctgca
     1201 tttgcgttag gtcctatcgg cttaggattg gttggctctg ctacagcaat gacagttgga
     1261 gcaatggggg catcaatgtt actaggagga gtttcccaaa tgctcaccaa aatgccaaaa
     1321 gcccccacaa tgggtaatga aacagaaaaa gaaagctcaa cggctttttc aaatttaaat
     1381 aacttggtgg ctcaaggcaa acctgtgccg ttggcttatg gtttgattcg taccggctcg
     1441 ctggtgatat cgcagggagt tgagacgatc accattaaag aaaaccaacc ggcaagcaac
     1501 aagaaaaccg gctttagaaa ataagggata attatgcgta tttacggagc aaaaggtggt
     1561 ggcggcagta gccatacccc gattgaagca ccggaaaccg gtcgctcaaa acagattgtg
     1621 aatattgtgg aattgctgtg cgagggcgaa attgaaggct tggttgatgg ctttaaatcg
     1681 atttacctag acggcacgca gatccaaaac gatgacggca cctataattt taataatgtg
     1741 agcggtcagc taaatgtcgg tacgcaagat caagatgtgt tggacggtta cgatagctca
     1801 cagaatgaag tgaatgtggg tgttgagatt aagaaaaaga atggggctat tgtccgcacc
     1861 gtaactgatg aacgaattaa ccgcttgcgt ttaacgttgg gagtaaggtc attattcca
//
LOCUS       DANLXQ010000256         1901 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.263, whole genome shotgun sequence.
ACCESSION   DANLXQ010000256 DANLXQ010000000
VERSION     DANLXQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.263"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            86..727
                     /gene="sodA"
                     /locus_tag="PXD94_002619"
     CDS             86..727
                     /gene="sodA"
                     /locus_tag="PXD94_002619"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL6287416.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
     gene            complement(790..1866)
                     /gene="alr"
                     /locus_tag="PXD94_002620"
     CDS             complement(790..1866)
                     /gene="alr"
                     /locus_tag="PXD94_002620"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL6287417.1"
                     /translation="MRLIKSFAPQQKLLAMIKANAYGQGLLPAAGTLADMVDGFGVAR
                     LREALEIQETGYTGKIVLVEGFFDREELLKTLSRKFDTVIHNLEQLELLEQVAKEWQA
                     EQQKGFWTRKAKIYFPINIWLKIDTGMHRLGIHPEQVDHFVERLKNCALVNSISFVSH
                     FSRADELDCGYTEKQIAVFEQATQKYQDHDRSISASSGILYWKQAHYDWVRPGIIMHG
                     ISPHYTPITDLGFKPVMTLSSSLIAVRTHKAGEPVGYGGAWVSPKDTKLGVTAMGYGD
                     GYPRNAPEGTPVLINGRIVPIVGRVSMDMLTVDLGAESQDKVGDKAIFWGEQLLIEDV
                     AKHIGVISYELITKLTPRVIFEYK"
BASE COUNT          544 a          411 c          415 g          531 t
ORIGIN      
        1 atgtgcattt tttgtgtgtt tcttgataaa atgtactcgg agtttaactc tgattaacct
       61 atttactatt tgagaggaat taactatggc atatacatta ccagaattag gctacgctta
      121 tgatgcgtta gagccacatt tcgatgctaa aacaatggaa atccaccatt ctaaacatca
      181 ccaagcttac atcaacaatg caaatgcagc attagaagct caccctgaat tattagaaaa
      241 atgtccgggt gcattaatca aagatttgag ccaagtgcct gcagaaaaac gtattgcagt
      301 gcgtaacaac gtaggtggtc atgtaaacca cactttattc tggaaaggtt taaaaacagg
      361 cactacctta caaggtgcat taaaagaagc gattgagcgt gatttcggtt cagttgaagc
      421 tttccaatca gaatttgaaa aagcagcgac tactcgtgtt ggttcaggct gggcgtggtt
      481 agtattagaa gagggtaaat tagccgttgt ttcaaccgct aaccaggaca acccactaat
      541 gggtaaagaa gtggcaggcg tttcaggcta tccgatttta gtcttagacg tttgggaaca
      601 cgcttactac ttaaactacc aaaaccgccg tccggatttc attaaagcat tctggaacgt
      661 tgtaaactgg gacgaagccg ctcgccgttt tgaagaaaaa gtagcaacct gcggttgtgc
      721 gaaataatat tttcgatctt gaataagata caagcggtaa aatttgttga aaattttacc
      781 gcttgttgtc tatttatatt caaaaatcac acgaggagtg agtttagtaa tcagctcata
      841 gctaatcacg ccaatgtgtt tcgccacatc ttcaattaat agttgctctc cccaaaaaat
      901 ggctttatcg cccactttat cttggctctc tgcacctaaa tcgaccgtca gcatatccat
      961 cgaaactcga ccgacaatcg gcactattct accgttaatc aacacagggg taccttcagg
     1021 agcattgcga gggtagccat cgccgtagcc cattgcagtc acgccgagtt tggtatcttt
     1081 agggcttacc caagcaccac catagcctac aggctcgccg gctttatggg tacgcacagc
     1141 gatcagggaa gaggagagcg tcattaccgg cttaaaacct aaatcggtaa tcggcgtata
     1201 gtggggagaa atgccgtgca taataatacc cgggcgaacc caatcataat gagcctgttt
     1261 ccaataaaga ataccgcttg aggcggaaat actgcggtcg tgatcctgat atttttgcgt
     1321 agcttgctca aacaccgcaa tttgtttttc ggtatagcca caatcgagtt catcggcacg
     1381 gctaaaatga ctcacaaagc tgattgaatt gaccaaggca caatttttca aacgttcaac
     1441 aaagtgatcg acttgctccg ggtgaatgcc taagcggtgc attccggtgt cgatttttag
     1501 ccaaatgtta atcgggaagt agattttcgc tttacgagtc caaaagcctt tttgttgctc
     1561 tgcctgccat tcttttgcta cttgctcgag taattccagt tgttcgaggt tatgaatgac
     1621 ggtatcaaac ttgcgggaaa gggttttgag tagctcttct cggtcaaaaa aaccttcaac
     1681 taacacaatt ttgcctgtgt atccggtttc ttgaatttct aaggcttcac gcaaacgggc
     1741 aactccaaag ccatcaacca tatctgctaa tgtgccggca gccggcaata agccttgccc
     1801 gtaagcattg gctttgatca tcgccaataa tttttgctgt ggagcgaaag atttgatcag
     1861 gcgaatatta tggcggaggg cttgactatc aatggttgct g
//
LOCUS       DANLXQ010000257         1878 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.264, whole genome shotgun sequence.
ACCESSION   DANLXQ010000257 DANLXQ010000000
VERSION     DANLXQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.264"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..312)
                     /locus_tag="PXD94_002621"
     CDS             complement(<1..312)
                     /locus_tag="PXD94_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6287418.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAIILPNITELKYACLYENLDLICTQNDLSLILYLTHNRE
                     DR"
     gene            complement(474..1814)
                     /gene="gdhA"
                     /locus_tag="PXD94_002622"
     CDS             complement(474..1814)
                     /gene="gdhA"
                     /locus_tag="PXD94_002622"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL6287419.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
BASE COUNT          539 a          419 c          323 g          597 t
ORIGIN      
        1 tctatcttct cgattatgcg ttaaataaag aatgagagat aaatcatttt gcgtacaaat
       61 taaatcaaga ttttcatata aacaggcata tttcagctct gtaatattgg gcaatataat
      121 agcaatttta ttacttgtgc cactttttaa cgtagaggct tgaatatttt tacgataacc
      181 caacttttca acggcagctt gtactttttc tattttagct aagctaacat tactttttcc
      241 gtttaataca ttcgatactg ttcctaacga aacatttgct tcctttgcga tatctttcat
      301 cgtaatgatc atatactgcc ctttcacata ctgctaaaac tgagattggc atagatttta
      361 ccataattgg caaatgaaat acgatttcaa tttagcttgt ctctagctac aacctaaaat
      421 aaaaaaactg cctcgatata tcgaggcagt tcttgaataa tagggataat aaattaaaca
      481 ataccctgtt ctaacatcgc atttgccact tttttgaaac cagcaatatt cgcaccgttt
      541 acatagttta cgaaaccatc tgcttcagta ccattttcta cgcagttttc gtgaattgct
      601 ttcataatgt tgaataaacg ctgatctact tcttcacgac tccaagatag acgaattgca
      661 ttttggctca tttctaaacc tgaggttgcc acaccacctg cgttagccgc tttacccgga
      721 gcataaagga ttttcgcacc gataaagact tcaacaccac ctaatgtggt tggcatattt
      781 gcaccttctg ccacacaaat acagccgttt ttcactaatt ctttagcatc ttcggtatct
      841 aattcgtttt gggttgcaca aggtaaagcg atgtcacatt tcacacccca cggtttttga
      901 ccgtcaaagt attttaagcc ttgttctgtt gcatacacag ataagcgttc acggcgttcg
      961 tttttcaact ctaataatgc ttgtaattgt gcctctgtca taccggattc agggaataag
     1021 acataaccgt tagaatctga aaccgttaat actttaccgc ctttttgaat cactttttca
     1081 gctgcatatt gtgccacgtt acccgaaccg gaaattacca ctgattttcc ttcgatctgc
     1141 tgacctttag tcgctaacat agactctgca aaatacaccg caccataacc agttgcttcc
     1201 ggacgaatta agctaccacc ccaagtcagc gatttaccgg ttaatactga agtaaattca
     1261 ttgcgtaatt ttttgtattg accgaacata tagccgattt cacgacctcc cacgccgata
     1321 tcgcctgcag gaacgtcagt atcagcacca atgtggcgat ataattcgct cataaacgct
     1381 tggcagaaac gcattacttc ggcatcagat ttgcctttcg ggtcaaagtc tgaaccacct
     1441 ttaccaccgc ccattggcaa tgtcgttaac gcatttttga atacttgctc aaaggctaag
     1501 aattttaata cgcctaaatc aacggttggg tggaaacgaa taccgccttt gtatggaccg
     1561 attgctgagt tcatttgtac acggtaacca cggtttactt gcacttgacc tttatcatca
     1621 acccaagtga cacggaagct aattacacgc tcaggctcaa caatacgccc taataaacct
     1681 tgctgggtat atttcggatt tttagctaag aaaggggcaa ggctaccgaa cacttcttca
     1741 accgcttgat ggaaaggggc ttggttcgga tcacgttgtt tgattttttg gaaaagggcg
     1801 tttaaatcgc tcatattata ctcctgaata aattgatgat gttgtgtatt taagatgagc
     1861 gaagtctagc aagcaatt
//
LOCUS       DANLXQ010000258         1875 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.265, whole genome shotgun sequence.
ACCESSION   DANLXQ010000258 DANLXQ010000000
VERSION     DANLXQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.265"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(272..511)
                     /locus_tag="PXD94_002623"
                     /pseudo
     CDS             complement(272..511)
                     /locus_tag="PXD94_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(672..965)
                     /locus_tag="PXD94_002624"
     CDS             complement(672..965)
                     /locus_tag="PXD94_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287420.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYKHLKETGQIVV"
     gene            complement(1080..1679)
                     /locus_tag="PXD94_002625"
     CDS             complement(1080..1679)
                     /locus_tag="PXD94_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287421.1"
                     /translation="MSYGLSTFNINENFRSLSLQRKGRANFSNKIATIQAASDEIIVI
                     KYSDGQVCLLYRENGIATLATENATFAEYLIFSTNVPKSGGYGIEVYNSAGNVVFSSN
                     HKFLRPIEYVDLNPSKNVFRFTGQANRQYGIILSNFGFDFVINYDNGYSTSRSAKVSG
                     NFIEFGFCKYHYSGLYKTGMSYNSKAMYFNALVVDITGY"
BASE COUNT          569 a          348 c          321 g          637 t
ORIGIN      
        1 gtgtccgcga atgggtggct ggtgaacgta aaatcccttt cggggtttgg gctgatttga
       61 ccgaattggt taaagccaaa aaagctaact tatcatcaat tcttaaaaaa ttaacaatag
      121 attagcactg actttaaagc ccctctcggg gcttaattat agaaaataaa atcacaatgg
      181 ctaattttgc caaatttgcc tgttaaaacg tgcgaaaaat tacaaaaaac ggctagagct
      241 atagccgttt tgagtgcaga tgttaacaga gttaagctct agcacgtcct gggccttgat
      301 actgcacacc atcagttaaa tgatcttgat tttcggttgc tgctacagca gattctgatc
      361 ttacatttaa actttgtgac tgttgtaatg catcaataag tttatttacc actgttccga
      421 gtgttttaat ttgctcggtt tgctcattaa tagtctgctt taactcttgc agttttaatg
      481 attgctcatc cacaaccact aatcgacggt tagctcaatt tgaggctctg tgctgccctc
      541 tactatggcg tgtaaatcca gcctaatacc ctctaaattt acaggctcgc catttgtttg
      601 cctaaaaaaa aggcgataac ttacgtcatc gcctctgaaa aacttaaatt tatgcttttg
      661 catttagacc cctacactac aatctgcccc gtctctttta agtgcttata gatacgacct
      721 aacataatct gcgttggcgt tttgccaata tcctcttttg ttagcggtgc atctgtaatc
      781 gctttggctg attgagcatc aatatatttg tactcacttg tgatgattac aaagtttgtt
      841 acctcgccat cggtatcatc acctgtgcca acaacatact tagcattgag tgagccgtca
      901 tcttgagttg agtaacttgc gatagttgag tacattgggt ttaagatttt gttaaaagtt
      961 gtcataattt ttccttttgt ggattgttga taaaaaaaga ccgcttgtat tgctacaagc
     1021 ggcgatgaat gtgtaatcag aattttagtt cgcctctatt aaatgaaggc gtttaatgtt
     1081 taatagcctg ttatatctac aaccagagca ttaaaataca ttgcttttga attataactc
     1141 attcccgtct tatacaaacc agaataatgg tatttacaga acccaaattc aataaaatta
     1201 ccacttactt tggcagatct agatgttgaa tatccgttat cgtagttaat gacaaaatca
     1261 aagccaaaat tggataagat aatgccatat tgcctatttg cttgccctgt aaatctaaaa
     1321 acattttttg atggatttag atcgacatat tcaataggtc ttaaaaattt gtggtttgat
     1381 gaaaatacaa catttcctgc tgagttataa acctctatgc cgtaacctcc gcttttcggt
     1441 acatttgtac taaaaatgag atattcagca aatgttgcgt tttctgtagc taatgtggca
     1501 ataccatttt ctcgatatag caaacatact tgcccatcag aatatttaat gacaataatt
     1561 tcatctgacg ctgcctgaat agttgcaatt ttattagaaa agttagctcg tccttttctt
     1621 tgtaaggata aacttctgaa attttcattg atgttaaaag tagatagtcc gtaactcatt
     1681 taatatgctc catatacaat ctgaatataa tctgaatcat cagccggtag actaaaagga
     1741 aaaatacaaa caacttctcc tgaaatcgtt ttatcgttga tcgaaacatt ttctaaatga
     1801 ataagattgg gaatattcgt tttataacct gttgcttgtt ttcggtgaac tgaatgtaac
     1861 atactaatga taaat
//
LOCUS       DANLXQ010000259         1681 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.266, whole genome shotgun sequence.
ACCESSION   DANLXQ010000259 DANLXQ010000000
VERSION     DANLXQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.266"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            72..1025
                     /gene="msrP"
                     /locus_tag="PXD94_002626"
     CDS             72..1025
                     /gene="msrP"
                     /locus_tag="PXD94_002626"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL6287422.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            1025..1609
                     /locus_tag="PXD94_002627"
     CDS             1025..1609
                     /locus_tag="PXD94_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL6287423.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
BASE COUNT          526 a          323 c          336 g          496 t
ORIGIN      
        1 ttattttgta agaattctag atcagctgag tccaaaataa taacaacaat tcataaaaaa
       61 aggatagttc gatgaaaaaa ttaacaattc aagatgttac acccgaaact attttctacc
      121 aacgccgtaa aattattacc gcgttaggtt taggtattgc agcttctgca ttgcctaaag
      181 taagtatggc agaaacacaa agacacttgc aatcactaca gtttaaaccg gatccaaata
      241 aagggcttga actcactccg gaaaacaaag taataggcta taacaatttc tatgagtttg
      301 gcgtggataa aagtgcacca gcaaaaaatg cggcaaactt caaaaccgat ccttggacat
      361 tggaaattgg cggagaagtt gaaaatccgt ttagcttaaa tcatcaacaa ttgcttaata
      421 cgtttccact agaagaacgc atttaccgtt ttcgctgtgt tgaagcgtgg tcaatggttg
      481 tgccgtggat cggctttgag ttagctcgct taattgagat ggcaaaacca accagtaaag
      541 ccaaatatgt ggtctttcac actttatatg atcctgcaca aatgccgggg caaaaaaatc
      601 gcttgctagg cggtgggatt gattatcctt atgtagaagc tttacgctta gatgaagcct
      661 taaattcatt aacactacta tccgtcggtt tatacggtaa aaccctgcca ccgcaaaacg
      721 gtgctcctat tcgcttagtc gtgccttgga aatatggctt taaaagtatt aagtcgattg
      781 tgaaaatcac ctttgctgaa aaacgcccac tcactacttg ggaaagtctt gctccaaatg
      841 aatatggctt ttatgctaat gtgaatccaa atgttgatca tccacgttgg tcgcaagcat
      901 ctgaacgagt aattggtgca ggcgggttat tatccgtcaa acgccaacca acactaatgt
      961 ttaatggcta cgaaaatgaa gtggcaaatt tataccgtgg cttagattta aaggtgaact
     1021 actaatgcta acctggttac gtatactcat tcatcttggc tgtttattac cgctagtttg
     1081 ggtagcacag ctattatata cgggtaatga aacaatatta ggtgccgatc cgattaaaga
     1141 gctggagcat tttctcggtt atggtgcaat cgtcattttt tgtataatgt ttctacttgg
     1201 tattgcccta caatacctaa agaaaaacca gtatcaaatt ttacgccgtc cattgggctt
     1261 atgggcgttt gtatgggcag ctctacatat ttcaagctat ctattgttag aattaagcct
     1321 tgatgtaaaa ttatttttta gcgagcttgc cggtcgccca tatttgattt taggagcagc
     1381 tgcttttttc attctaagca taatggcaat aacttcgctg cctatgctaa aacgaaaatt
     1441 aggaacacgc tgggggacga ttcaccagtg ggcttatcct gcattagtat tggcgatggt
     1501 acattattat tggtcggtaa agagcgtaac attcgatcca attatcatcg gagtcttagt
     1561 gctgtttatt gttgctcata aaatacaggc taaatttgca aaaaaatagc aaaatcacac
     1621 cgcttgcagc agtcaaacta catacaaaaa ataaggggct gtcctagata acgactaaaa
     1681 c
//
LOCUS       DANLXQ010000260         1678 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.267, whole genome shotgun sequence.
ACCESSION   DANLXQ010000260 DANLXQ010000000
VERSION     DANLXQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.267"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            12..698
                     /locus_tag="PXD94_002628"
     CDS             12..698
                     /locus_tag="PXD94_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6287424.1"
                     /translation="MDTLASRLKIVLDQKGLTQEKFAEQIGVSQPSVFKILNGQTRNP
                     TRIYEIANVLDVDVNWLKTGKGEAPDFAKIGKNPTAYEEESSMLRLEVLDVYASAGNG
                     SFVTGDLTAYTHAVEFENAYFAQVFQRANAKGLSIINVDGDSMEPTIGNGDLLFVDTT
                     KSAYQGDGVYVFSYGENLYVKRLQFAGDELLVISDNPLYKEWRITSENEHKFQIHGKV
                     EFMQGHIRRV"
     gene            702..1220
                     /locus_tag="PXD94_002629"
     CDS             702..1220
                     /locus_tag="PXD94_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287425.1"
                     /translation="MEHQNLTHLVYITSHKQIGVYSISNLNIDDLYFKGYCIQKNRVI
                     TLRADRIIKQFDDLALAENYAKNIPQDVFYLFDSLLNQQRKEKITPIYKIGLCFTGFK
                     QARKNELIQLAVDNDLRVVQNVTGAVDFLIFDKESKTVGPAKLAKAEKLGIKIIDDEE
                     FLYMLETGVVPD"
     gene            1238..1648
                     /locus_tag="PXD94_002630"
     CDS             1238..1648
                     /locus_tag="PXD94_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287426.1"
                     /translation="MFIPKSSKLKTNNYIEELNIKSIGETDMKKALTLLALSLFALPV
                     QADSLELARNKVKQIFISDEEPKVKDATWTAPFIFKVGVFDDGTKRDGYAEYVCQVLY
                     DHGFKGKGVLVSVIDIKKLVQTGDWVDLGKAKCQ"
BASE COUNT          580 a          275 c          333 g          490 t
ORIGIN      
        1 aggagttcct aatggatact ttggcaagtc gtttgaaaat cgttttagac caaaaaggtt
       61 taacacagga aaaatttgca gaacaaatag gtgtttcaca gccatctgtt tttaaaatcc
      121 tgaatggaca aactcgaaat cccacaagga tttatgaaat tgcaaacgtc ctagatgtcg
      181 atgtaaattg gctcaaaacc ggcaaaggcg aagcaccaga ttttgcgaaa atcggaaaaa
      241 atccgaccgc ttatgaagag gaaagctcaa tgcttcgact tgaggtgtta gatgtatatg
      301 cctctgccgg caatggtagt tttgtcacgg gagacttaac cgcttacacg cacgcagtag
      361 aatttgaaaa tgcctatttt gcacaagtct ttcaacgtgc aaatgccaaa ggcttatcaa
      421 ttattaatgt agatggcgac tcaatggagc caactatcgg taacggtgat ttgctgttcg
      481 tagataccac taaatccgct tatcaaggcg atggtgtcta tgttttcagc tatggcgaaa
      541 acttatatgt aaaacgcttg cagttcgccg gtgatgaatt gttagtcatt tccgacaatc
      601 cgctttataa agagtggaga atcacatcag aaaatgagca taaattccaa attcacggta
      661 aagtggaatt tatgcaaggg catattagga gggtttaggt gatggaacat caaaatctaa
      721 ctcatcttgt ctatattaca tcacataaac aaattggagt ttattcgatt tctaatttga
      781 atattgatga cctttacttc aaaggttact gtattcagaa aaatagagtt atcacattaa
      841 gagctgatcg cattattaaa caatttgatg atttagctct tgccgaaaat tatgccaaaa
      901 atattcctca agatgtgttt tatctatttg attctcttct gaaccagcaa cgaaaagaaa
      961 aaataacacc gatctataaa atcggtttgt gttttactgg atttaagcaa gctagaaaaa
     1021 atgaattaat tcaactagca gtagataatg atttgagagt agtacaaaac gtaactggtg
     1081 cagttgattt tttaattttc gataaagagt cgaaaactgt tggaccagca aaactagcaa
     1141 aagctgaaaa gttaggaatt aaaatcatcg atgatgaaga atttttatat atgttagaaa
     1201 ctggcgttgt gccggattag gtttgacttc tcaatagatg tttataccaa aatcatctaa
     1261 actaaaaact aacaattaca tagaagaatt aaacatcaaa tcaatagggg aaaccgatat
     1321 gaaaaaagca cttacactac ttgcactatc actatttgca ttgcctgtac aagccgatag
     1381 cctcgaactt gctcgtaata aagtaaagca aatttttatc agtgatgaag agccaaaggt
     1441 aaaagatgcg acttggactg caccatttat ctttaaggtt ggagtttttg atgacggcac
     1501 gaaaagagat ggctacgccg aatatgtttg ccaagtttta tacgaccacg gatttaaagg
     1561 taaaggtgtg ttggttagcg taatcgatat aaaaaaacta gtccaaacag gtgattgggt
     1621 tgatcttggc aaagctaaat gtcagtaata cataagggac agattattct gtctcttt
//
LOCUS       DANLXQ010000261         1651 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.268, whole genome shotgun sequence.
ACCESSION   DANLXQ010000261 DANLXQ010000000
VERSION     DANLXQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.268"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(80..574)
                     /locus_tag="PXD94_002631"
     CDS             complement(80..574)
                     /locus_tag="PXD94_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6287427.1"
                     /translation="MSRKYKFHEKSGAYFVSFATVYWVGVFVREIYFQVIVDALDYCR
                     KHKGMILYAYCIMPTHIHLLFQAENQNPSDLIRDFKTFTAKILIQRIKDNLQENRREW
                     LLWMFSRAAERRCNVKQYQFWQHHNQPIEIYSQKFFDEKLNYIHQNPVVSGFVSEPWE
                     WKYN"
     gene            complement(1178..>1651)
                     /locus_tag="PXD94_002632"
     CDS             complement(1178..>1651)
                     /locus_tag="PXD94_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein"
                     /protein_id="HDL6287428.1"
                     /translation="LFIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQNTNQPVS
                     FTDASSRVDAASHYGFKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGVAKYYGK
                     SKRIAIDEFNYTYYPGTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILKLEL"
BASE COUNT          558 a          311 c          249 g          533 t
ORIGIN      
        1 atttacgcaa aattctccga cagacaagta aaaattcaca atttttcgac agagatcgca
       61 aaaatacaag cagtttattt caattatatt tccattccca aggctcagac acaaaaccac
      121 ttaccactgg attttgatga atataattta acttttcatc aaagaatttc tgcgaataga
      181 tttcaatagg ttggttatga tgttgccaga attgatattg ttttacatta caacgtcgtt
      241 cagccgcccg agaaaacatc cacagcaacc attcccgacg gttttcttgc aaattatctt
      301 taattctctg aataagtatt ttagcggtga atgttttgaa atctcgtatt aaatcagaag
      361 gattttgatt ttctgcttga aataataaat ggatatgagt tggcataata caataggcat
      421 acaaaatcat tcccttatgt tttctgcagt aatctaaggc atcaacaatt acctgaaaat
      481 aaatctctct cacaaacaca ccaacccaat aaacagttgc aaaactgaca aaataagcac
      541 cgctcttttc gtggaattta tattttcggc tcatagcctt tctcaactta aaaaggaata
      601 agaaaaatac cctattcaaa attaaattcc acaagccaat ataacttttt cgatctagat
      661 cgcaaaaatc attaaaactt gcaaagtgtt gataaaattt aaccgcttgt gataagccaa
      721 agaaatactg aaatgatagc aatggatgga taaataaaat gatgacgcaa gcgtccacgc
      781 ttgtgccaaa aacaaaagcc caaataaacc aaattttact tggctgattt aggcttttat
      841 cttatgactg aaatactttc agaacaggaa ttaactggca caagcgtgga cgcttgcacc
      901 atctgggtta aatccttata ttataaggtt ttaacctact gtgcagactc aattttagtc
      961 actatgccaa ataattagtt aaaggaaatt taagctagct tatctaggac aaccccattt
     1021 agaatttata acttgtgcta aacactatag ttctccctct tgcataatta ttgagcgtat
     1081 aaataggttc atcactgata tcagaattgt aaaccctctg acttgctgca tcattattag
     1141 catcaagagg atcaatgtac tttttgtcaa agacatttta tagttctaac tttaatataa
     1201 gcgacttgat tggttcgtag ctcacgtaaa aatcaacaat aataggttgt ttatttacgg
     1261 tttcttcaag ccgatgagtt tctttcacaa tatgtgttcc aggataataa gtatagttaa
     1321 actcatctat cgcaatacgt ttactttttc cgtaatattt tgccactccg ccaatttgta
     1381 gtctttgatc aaaccatctt gtccctaact ccaatgagcc atagtctctt ggtaacgctg
     1441 aaattttgct ggcaccaaaa ccttgagttt taaaaccata atgagaagcg gcatctaccc
     1501 ttgaacttgc atctgtaaag gatacgggtt gattcgtatt ttgacgagca taagctaagt
     1561 tgccatagaa agagcctata tcataactaa attctaattc tatccctttg atatttactg
     1621 gtttttcata gtttttgtgg aaaatgaata a
//
LOCUS       DANLXQ010000262         1646 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.269, whole genome shotgun sequence.
ACCESSION   DANLXQ010000262 DANLXQ010000000
VERSION     DANLXQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.269"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..671
                     /locus_tag="PXD94_002633"
     CDS             <1..671
                     /locus_tag="PXD94_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287429.1"
                     /translation="RNPLIYHRHSWHLEFDGSVLYIADPTLFKYESINLAWYVGDKDT
                     DLYPYLAEFVTSVATHLNVETDHIVTYGSSGGGFAALQLGARIGNGVVTVCINPQTNI
                     MKYVPSHRNAFLNACFDFNAEEYDSLSNQEKFNALVLLKENTSKILYIQNTQDEFHHK
                     WHFLPFLELKGIDLSEIEKTHWREQQSKDVKVILYSHESGHAAEPKEMIPEILSAVDQ
                     LIAS"
     gene            673..>1646
                     /locus_tag="PXD94_002634"
     CDS             673..>1646
                     /locus_tag="PXD94_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6287430.1"
                     /translation="MLFYLVSDSLTRLSLSNESLVDLGRITSIGNYPFLHNAKNAKFL
                     MVESAWQGYKNRWKYKIASYPDNPERTNEKLVRLVEKAKDKGIPTVFWNKEDSVHFDR
                     FIDSAKHFDHIFTVDQNAIARYREVVPATTTVNVGMFPVQPKIHNFQGFNFKHLAANF
                     VGSYSHHIHNKRRERQDMLFGAALKAGLPVTIFDRNSDRKSENYRYPKNSFNLNVQPN
                     VSYTETANIYRDYLVSLNINTVEDSPTMFSRRVVEILACGGILVSTPSMAIDNIFKEY
                     CHIVQSEDEAIELFQRLKRGASRDDLNKAIAGSEFVLSNFTWEHFLSQ"
BASE COUNT          535 a          270 c          306 g          535 t
ORIGIN      
        1 ccagaaatcc cttaatttat catagacata gttggcatct agaatttgat ggctcagttc
       61 tatatatcgc agatccaaca ttatttaaat atgaatcaat taatttggct tggtatgttg
      121 gagataaaga tactgattta tatccatact tagctgaatt tgtaacttct gttgctacac
      181 atttgaatgt tgagacagac catattgtta cttatggttc aagtggtggt ggatttgctg
      241 cattacagtt gggagcaaga attggaaacg gtgtagttac cgtttgtatt aatccacaaa
      301 ccaacatcat gaaatatgta ccaagtcatc gtaatgcctt tttaaacgct tgctttgact
      361 ttaatgcgga agagtacgat agtctttcta atcaagagaa atttaatgct ttagtattac
      421 ttaaggaaaa tacatcaaag attttgtata ttcaaaatac tcaggatgaa tttcatcata
      481 aatggcattt cctcccattc ctagagttaa aggggataga tctatcggaa atagaaaaga
      541 cccattggag agaacaacag agcaaagatg taaaagttat tttgtattct catgaatcag
      601 gtcatgcagc tgaacctaag gaaatgattc ccgagatatt atccgctgtt gatcaactga
      661 tagctagcta aaatgctgtt ttatttggta tctgattctt taactaggct ttcattatca
      721 aatgaaagcc tagttgatct tggaagaata acatctatag gaaattatcc tttcttgcat
      781 aatgcaaaga atgctaaatt tcttatggtc gaatccgcct ggcaaggcta caaaaaccgt
      841 tggaaatata aaattgcctc ttatcctgat aatcctgaac gaaccaatga aaagttggtt
      901 cgtttagtag aaaaagcgaa ggataaaggc attccaacgg tcttttggaa caaagaagat
      961 tctgtgcatt ttgatcgatt tattgattcg gcgaagcatt ttgatcatat ttttaccgta
     1021 gatcaaaatg ccattgctcg ttatagggaa gtagtgcctg cgacaacaac cgtcaatgtc
     1081 ggaatgttcc ctgttcagcc caaaatccat aactttcaag gatttaattt taaacacctt
     1141 gccgcaaatt ttgtagggag ttacagtcat catatccaca ataagcgtag agaaagacag
     1201 gatatgttgt ttggtgcagc acttaaagca ggtcttccgg taaccatctt tgatagaaac
     1261 tctgatagaa aatcggaaaa ttatcgttac ccaaaaaata gctttaattt aaatgttcaa
     1321 cctaatgtta gttatactga aactgctaat atttatcgtg attatcttgt ttctttgaat
     1381 attaatactg tcgaagattc ccctacgatg ttttctcgca gagtcgttga aatattagct
     1441 tgtggaggaa tattagtctc tacaccaagt atggctatcg acaatatatt taaagaatat
     1501 tgtcatatcg tacaaagtga agatgaggct atagaattat tccaacgttt aaaacgaggt
     1561 gcttctcgtg atgatttgaa taaagcgatt gcaggctctg aatttgtatt gagtaacttt
     1621 acttgggaac atttcttatc ccaaat
//
LOCUS       DANLXQ010000263         1612 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.270, whole genome shotgun sequence.
ACCESSION   DANLXQ010000263 DANLXQ010000000
VERSION     DANLXQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.270"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            38..>1612
                     /locus_tag="PXD94_002635"
     CDS             38..>1612
                     /locus_tag="PXD94_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL6287431.1"
                     /translation="MEKMMRKINQLIISPYFFLSILSASANSDFWKSDLNFNLTNITK
                     RVGVDNFTTPVAGEPWAGIGPNGEAAGTAPLYYSRIPAMQVTEDNKLVVMFDLRWNRA
                     YDQDRIDPGIAISSDGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGT
                     WSMSDQQWYGGRVPHFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGK
                     PTLGFLGGVGSGIVMQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPN
                     QSSLENMVFELEPGKLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSS
                     IYVTLPNGRKVLLISKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGA
                     GYSSLAYKEGNLFVAFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALP
                     YLNKAQKETLVEKMRRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQN
                     IYQRALAYVNKVTNTAD"
BASE COUNT          514 a          299 c          330 g          469 t
ORIGIN      
        1 ttcagctaaa aattagtctt tgcactttat ccccctaatg gagaaaatga tgagaaaaat
       61 caatcagtta atcatatccc catacttttt cctaagtatt ttatctgctt ccgctaactc
      121 tgatttttgg aaaagcgacc ttaattttaa tctcacaaat attaccaagc gtgtcggagt
      181 cgataacttt accactccag tagctggcga gccttgggct ggtattggtc caaatggtga
      241 agctgcaggt actgcgccat tatattatag ccgtattcca gctatgcaag ttaccgaaga
      301 taacaaatta gttgttatgt ttgatttacg ctggaatcgt gcgtatgacc aagatcgtat
      361 tgatcctggt attgcgattt cttccgatgg tggccatact tggacgaaga aaaccgcttg
      421 gtcttttgat agaactaatc acccagcacg ccgttcaatg gatccaacgc tattacataa
      481 tcctattgat aatagtttat atgtaatgca cggaacttgg tctatgtcgg atcaacaatg
      541 gtatggtggg cgtgttcctc actttaatag tggcagctgg gcagctacca tttataaatc
      601 tactgatggt ggtttaaatt gggagaaaaa tactgagttt agtaaattca gcaatttaga
      661 cgtattttct aaagtcacca aaaatggaaa acctacctta ggctttttag gcggggtcgg
      721 atctggaatt gtaatgcaag atggtacgtt ggttttccca attcaaaccg cacatcaaaa
      781 tggtattgcg accactatta tgtattctaa ggataatggg aaaacttggg aaatgccaga
      841 aattgacaac ccagttgcac cgaaccaaag ttctttagaa aatatggtgt ttgaactcga
      901 accgggtaag ctagtgatga ctggacgagg aaacagtcgc tgggcatact cttctactga
      961 tatgggtaag acctgggaat tatttactcc aactaatgga ttattaccaa ctagctcaca
     1021 accaactcaa ggctcttcta tctatgtaac actaccaaat ggtcgaaaag tgctactaat
     1081 ctctaaacca gatggaaaga atgatgggtg gaaaaggggt gatattacac tttggatgct
     1141 agatgcaaaa gatccatcgc acaaacacaa ggtgcatatt attcgtccag gttcaggtaa
     1201 tgccgctggt gctggttatt cctctttagc ctataaagaa gggaatttat ttgttgcttt
     1261 tgaaaacgat ggtgatattt cagtaaaaaa tttaactgaa catattgctg aaatggaagc
     1321 aaaagctatc gaatggggat taccagatga acttactcct gctttggata aaatcaatgc
     1381 actgccttat ctcaataaag ctcaaaaaga gacgctagtc gaaaaaatgc gtcgtgctaa
     1441 tgatactgcg attgcacaat cttttgtgat taacaaagaa atgtataatc tgaaaaacaa
     1501 tagtgatgag ctggataaat tggcaaaaaa tattactaaa gcactgccgt ctcaacagaa
     1561 tatttatcaa agagccttag cctatgtgaa taaagtcacc aatacagcgg at
//
LOCUS       DANLXQ010000264         1603 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.271, whole genome shotgun sequence.
ACCESSION   DANLXQ010000264 DANLXQ010000000
VERSION     DANLXQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.271"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            201..1520
                     /locus_tag="PXD94_002636"
     CDS             201..1520
                     /locus_tag="PXD94_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL6287432.1"
                     /translation="MNHLLYSVEDKPPFGLSLLLASQHLLAALGGIIAVPLVLGNVLK
                     LSTADTIILVNAALLISGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESG
                     IAGIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQR
                     TDPHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNG
                     VKEAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHL
                     RGGVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPV
                     FGALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTR
                     PELLDKLPHFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
BASE COUNT          442 a          301 c          321 g          539 t
ORIGIN      
        1 ttttttgtta caactggaaa gaaattggta caaacattcg gattagcttt gcaaaaagcg
       61 taaaaaatct taccgcttgt catttatcag ttagggaaaa taaaaaatca ctctcttata
      121 taaaaacaat tacgcaaacg attgcttatt aaaaaaactt cagtataatg ccgacatttt
      181 ttaactctcg aggaatcact atgaaccatt tactttattc tgtcgaagac aaaccgcctt
      241 tcgggctgag cttattgctt gcctcacaac atttattagc cgctttaggt ggcattatcg
      301 ctgtaccttt agtgctaggg aatgtactca aactctctac tgcggatact ataatcttag
      361 ttaatgctgc acttttaatt tcaggcatcg tcactattat tcaatgtcga ggtattggcc
      421 caatcgggat tagactaccg agtgtaatgg gaactagctt tacgtttgtt gcagcagcat
      481 tagcgattgg tttcagtgaa agtggcattg ccggcattat gggagcctct ttagttggct
      541 ctctagtgat gattatcggc agttttttta tgccttatat ccgtaagctt tttccacccg
      601 tggtaacagg tactgtcata atgatgattg gcctaagttt aattcctgtt gcagtagact
      661 ggtttgctgg tggtcagcgt actgatccac actatgcaac cccagaaaat cttataatgg
      721 ctagttttgt attaattatt gtcgttgctt tagtacaatg ggggaaaggt attttttcag
      781 ctgctgctat tgtgattggt atgatgatag gttatatcgt ttcgctagct ctgggttggg
      841 taagttttaa tggcgtgaaa gaagcacaac tttttgcgtt accacaacct ctacattttg
      901 gcttagaatt tccaatctcc ggtatcatcg gaatgtcaat tgcttattta gttaccattg
      961 tagaatctag cggaaatttc ttagcactag gcaatgcaac taaaactgaa attaccggaa
     1021 aacacttacg tggaggtgta ttatgtgatg gtttaggctc agcatttgcg gcaattatgt
     1081 ccacaactcc cttttcttct ttctcacaaa atattggggt catttcatta accggtgtag
     1141 ccagtcgcta tgttgtagca tttacaggcg cattacttgt actagccggt ttatttcctg
     1201 tatttggagc attaatcgtg tctatcccgt tacctgtgct tggcggtgca ggcttaatga
     1261 tgtttgccat gattatcgca gccggtatcc aaatgttaga taatattcaa cgtagcaaac
     1321 gaaacggttt aattattgct atctctatcg gttgtgggct ggccgtaaca actcgacctg
     1381 agctattgga taagcttcca cacttcttta aagaagtttt tggctcaggt attacggtcg
     1441 gctccttatt agcacttatt ttgaatttag ttttacctga agataaaata accaagaaag
     1501 aaaataagag tgatgaataa atcaccctaa aaaaacaagg acagctcaca atacaacttt
     1561 gatgctgtcc tttattccca tagagagcat atttaaattt agg
//
LOCUS       DANLXQ010000265         1590 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.272, whole genome shotgun sequence.
ACCESSION   DANLXQ010000265 DANLXQ010000000
VERSION     DANLXQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.272"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..371
                     /locus_tag="PXD94_002637"
     CDS             <1..371
                     /locus_tag="PXD94_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287433.1"
                     /translation="DFGKQLAKPVKFIKQRTAFLPKPLQQAVVFAVIFAGLPLFLVGF
                     TVLRILNGTPVEDISTNDVAYNPNHVENTVNEIEDDSEDSKPEDLWDVIADQDENQLD
                     GYRDGYFGYGLYQGGYFVKR"
     gene            complement(424..>1590)
                     /locus_tag="PXD94_002638"
     CDS             complement(424..>1590)
                     /locus_tag="PXD94_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL6287434.1"
                     /translation="QMNSELGNQQARVPLWWGLFHQINKGVTHALTASIPCKPDLRQV
                     RFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQSSQLSLTPAQVRETSWLGGRILLT
                     NSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANTGLKDRLLADVK
                     QNFSAKVIDFFSSKENADESILRTVLRAENMDISRGKVYPGYGGNLDPTFANATTRLT
                     SSVGSLAASFAAFPAFDAMRQALPMVHAFMLMAVIIITPLVIVMSGYSLKTLVTLTFV
                     SFALVSLTFWWELARWLDSWLLSALYDSSAHSRINPYFLENTQDDFIVNFVMGSMFLV
                     LPAIWLGSMSWAGVKIGDIAAGFTTDSLDSKSAGSQGGNLVKKGGKEISAGFNK"
BASE COUNT          493 a          336 c          306 g          455 t
ORIGIN      
        1 tcgatttcgg aaagcagctc gctaagccgg ttaaatttat caaacaacgt accgcatttt
       61 taccaaaacc gctacaacaa gctgtcgttt ttgcggtgat ttttgcagga cttcccttgt
      121 ttttagttgg attcacggtt ctgcgaattc tcaatggaac accagtagaa gatatttcaa
      181 caaatgacgt agcatataat ccaaaccacg tcgaaaacac ggttaatgaa attgaagatg
      241 atagtgagga tagtaagccg gaagatttat gggatgtaat tgctgatcaa gacgaaaatc
      301 agcttgatgg atatcgtgat ggatattttg gatatggact ctatcagggt ggctattttg
      361 taaaaagatg agaaaatatg attagaaatt aataaggtag agattaacct ctaccttatt
      421 gttttatttg ttaaaccctg cagatatttc cttaccgcct ttcttaacta aattaccacc
      481 ttgagagcct gcggatttac tatctaatga atcagtagta aaaccggcag ctatatctcc
      541 tatttttact ccagcccacg acatactgcc tagccaaatc gccggtaaaa ccaagaacat
      601 cgaacccatc acaaaattga cgataaaatc atcttgcgta ttttcaagga aatacggatt
      661 tattcgacta tgtgcactgg aatcatatag agcggatagt agccaagaat ctaaccatct
      721 tgctaattcc caccagaatg tcagtgatac caaagcaaaa ctcacaaaag ttaatgtaac
      781 taatgttttg agagaataac cactcatcac aataaccagc ggagtaatga tgatcacagc
      841 catcaacata aaagcatgta ccatcggcag tgcttgtcgc attgcatcga atgctggaaa
      901 ggctgcaaag ctggcagcta agcttcccac tgaagatgtt aatcgagtcg ttgcatttgc
      961 aaatgtagga tctaaattcc ccccataccc gggataaact ttccctctcg aaatatccat
     1021 attctctgct cgtaatacag tacggagtat ggattcatcc gcattttctt tgctgctaaa
     1081 aaaatcaatc acttttgcag agaagttctg tttgacatca gctaataaac gatcttttaa
     1141 accagtattc gccgtatccc accactctgc acaatttgga aaaccacctt ccccattatt
     1201 cggcaatcca ttatcccggg aagcattgta gggaaataga ctatgaggtt gttgagctcg
     1261 ataacgggga taaagctcac tgtttgtcaa aagaatacga ccacctaacc agctggtttc
     1321 acgcacctgt gccggagtca atgagagctg actgctttga attttctgac gtgcaggtac
     1381 ataacattgt tgtacaaata agcgaacctc agtcaataat gcaggattat tgattttttc
     1441 atgttggacc tcaaagcgaa cttgacgtaa atccggttta cacggaattg aagctgttaa
     1501 tgcgtgtgta actcctttgt tgatttgatg gaataatccc caccaaagcg gtactctcgc
     1561 ttgctggttg cccaattcag agttcatttg
//
LOCUS       DANLXQ010000266         1584 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.273, whole genome shotgun sequence.
ACCESSION   DANLXQ010000266 DANLXQ010000000
VERSION     DANLXQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.273"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            38..292
                     /locus_tag="PXD94_002639"
     CDS             38..292
                     /locus_tag="PXD94_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287435.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFCNYQQFMPEVEAKESLR
                     KQEPELDDRYNEEEYSEEEYPDENDASDENDNYDETYNVF"
     gene            492..668
                     /locus_tag="PXD94_002640"
     CDS             492..668
                     /locus_tag="PXD94_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287436.1"
                     /translation="MATSSFDKRFVVKDPKAVKVLIEALQSPIKVVLPKKDLEKEEKE
                     ALCAIKKRLASMGL"
     gene            629..1180
                     /locus_tag="PXD94_002641"
     CDS             629..1180
                     /locus_tag="PXD94_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287437.1"
                     /translation="MCNQKTLSLYGLVKELGIEKTQLFLEQAFSCSRNKDVESFLLNK
                     AIRFETALAAATHLIINEQGDILAYFTLSFKEIEVEVSKSQLKKLTAGLSNDSHLRVL
                     LIGQIGKNEKVIDNPAKLPDILNHIYAQLYKAQRVLGGRVVILECENTPKLLELYKQN
                     KFQILNTIGSENGLKTLFFIPDF"
     gene            1250..1480
                     /locus_tag="PXD94_002642"
     CDS             1250..1480
                     /locus_tag="PXD94_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287438.1"
                     /translation="MSLLKKAQTFISIFNDVRTKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAVETYDNV"
BASE COUNT          587 a          262 c          284 g          451 t
ORIGIN      
        1 ttcgatgtcg ctcacttatg ccacccctca attagggatg atatgccacg ctgatcctga
       61 atatgtaaaa tacaactacg ccggactttg ggaaaaaggc agcgatggca cttttttctg
      121 caattaccag caatttatgc ctgaagttga agctaaagaa tcactaagga aacaagaacc
      181 agaacttgat gatagatata acgaagagga atatagcgaa gaagaatatc ctgatgaaaa
      241 tgatgccagc gatgaaaacg ataactacga tgaaacttat aatgttttct agtatgtata
      301 tggaaaccgg aggaatccgt gattgaaaag gcttgcattt ccaaatatag tctatatatt
      361 gtggtaattg aaagtatttc tatgctaaca agcataaggc tatgtaatcg aaaaccaccc
      421 acaaggccta aatacagtat tattacagcc atgagtaacc atggtaattt aaatatttaa
      481 ggagttctat aatggcaaca tcatcatttg ataaacgctt tgtagtcaaa gatccaaaag
      541 ctgtcaaggt attaattgag gcattacaaa gcccaatcaa agtagtttta cctaaaaaag
      601 acttagaaaa agaagagaaa gaagcactat gtgcaatcaa aaaacgctta gcctctatgg
      661 gcttgtaaaa gaactaggta ttgaaaaaac acaactcttt ttggagcaag cattttcttg
      721 cagtagaaat aaagatgttg aaagttttct acttaataaa gcaatcagat ttgaaaccgc
      781 attagcagca gcaacacatt taatcattaa tgaacagggc gatattttgg cttactttac
      841 cctctctttt aaagaaattg aggtggaagt aagcaaaagc caattaaaaa agctaactgc
      901 tggtttaagt aatgattcac atttaagagt attgcttatc ggtcaaatcg gcaaaaatga
      961 aaaagtaatt gacaatccag ccaaattgcc agatattcta aatcatatct atgcacaact
     1021 ttataaagca caacgagttt taggtgggcg agtcgttata ttagaatgtg aaaatacgcc
     1081 taaattatta gaactatata aacaaaataa atttcaaatt ctcaatacca ttggaagtga
     1141 gaatggtcta aaaacacttt tctttattcc tgatttttaa ctacagctaa tatttcatta
     1201 gaagaaagtc gccagctttg tcaggaagtc atatatggga gaaagaacaa tgtctctact
     1261 taaaaaagca caaacgttta tttctatttt taatgacgtg cgaactaaaa atgaagctca
     1321 acgccagtct tcaaacaatt taaatgttgc cttgaaaaat gctgctgaaa tgagagctga
     1381 gatgaatggg ttctccctta agcctcaaaa gcgttctact tactttgctg accattatgg
     1441 ttgtggtatt catgcagtag aaacctatga taatgtgtag aagatcagac ttgatctgac
     1501 aattcactct aaaaagtgag agtttcccgt ttagaatatg agtgtccaaa ttcaatctaa
     1561 acaaaaaagg aaactctcat gttt
//
LOCUS       DANLXQ010000267         1552 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.274, whole genome shotgun sequence.
ACCESSION   DANLXQ010000267 DANLXQ010000000
VERSION     DANLXQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.274"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            232..609
                     /locus_tag="PXD94_002643"
     CDS             232..609
                     /locus_tag="PXD94_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6287439.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAVGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
     gene            1198..>1552
                     /locus_tag="PXD94_002644"
     CDS             1198..>1552
                     /locus_tag="PXD94_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL6287440.1"
                     /translation="MTISIRQLAKKHCTYIPDEDFPNDPELGEFNCPDVNFPHTATGK
                     HKRINYSATLSAYIHDLFTPFISYSQTHRAPNIKEIFFSNLGDYGVNTALQPEQAKTT
                     QFGINGFKERVFSEED"
BASE COUNT          549 a          273 c          293 g          437 t
ORIGIN      
        1 gatcaaaaaa tatgtatttc acctgactta tacaacactt atctgcccct tacctagggt
       61 attcgcagag gaaggttata tgttagatac ggttgaggtt aaagcccaag caataaaaaa
      121 acagatgaag ttttcattaa aacaggggca ataagcaccc gagataaaat cttggaatca
      181 aaccaaagct tagagactat tgttcgttct atgcctggcg catttacaca aatggataaa
      241 tctcaaggtg tggtttctgt taatattcga ggaggttctg gatttgggcg agctaataat
      301 gcagtaggcc caaattatat gggaatgctc gctggcaaat accaattcaa tgactctact
      361 tggataggag cattatttgg gcatagttgg cgcaaaatct ctcaaaatta taaaatagga
      421 ggcggacgaa aagttacgga tagctcgatt gaccttagta aaattgatga tctagataaa
      481 gaaagttcta ccacctcacc gttcaatgca gctcatttaa aacaaaaacc aatgagtcgt
      541 atggctaaat tggaatatgg cgataaatac caaaccatca cactattacc aaagtcaggt
      601 agcgggtaga catttaaaaa accaaaacaa ccagttaaat tatcgttttc acttgcccga
      661 gtcaccttgg ttagatgttc aagtggccta tgcggaaaat caaggcaaac aaaactacaa
      721 caaagggagc tgaattaata ataaatattt aattcaaggt ttggcaatta acaacaaagc
      781 gacaacctta aatttgagta acacctttaa agtgtctccg tttaaagatg gagaatggga
      841 agcaatatta ggttttaatc gtttaaagaa ccgctatagt aaaaatagac atcctgcgga
      901 attaaactat aacttagaga atggcgaaac cagcgaaaac aacgattctg taggaatgac
      961 agccgttcgt aaatcgcttt atacttcaac tttccagcct gatggcgaac agaagttcga
     1021 tactttttat ctcgacaatc actttaacta tggtatatgg ggattggata taaacgcaaa
     1081 tattaatacc gctcggttta ttggtcagcg ttttaaatat tttccttttg cgattagaga
     1141 attggagtca gatattagac aggcaagtag aaataggcag tttgagcatt taaggtcatt
     1201 acaatctcaa ttagacaact tgcaaaaaaa cattgtacat atattccaga tgaggatttt
     1261 cctaatgatc ctgaattggg agagtttaac tgtcctgatg tcaattttcc tcatacagca
     1321 acaggaaaac ataaacgaat taattattca gcaaccttgt ctgcttatat tcacgattta
     1381 ttcacgccct ttattagtta ttctcaaaca catcgagccc ctaatattaa agaaatattc
     1441 ttctctaatt taggtgacta tggtgtaaat accgcactac aaccagagca agcgaaaaca
     1501 acacagtttg gtattaatgg attcaaagaa cgggtattta gtgaagaaga ta
//
LOCUS       DANLXQ010000268         1541 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.275, whole genome shotgun sequence.
ACCESSION   DANLXQ010000268 DANLXQ010000000
VERSION     DANLXQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.275"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..213
                     /locus_tag="PXD94_002645"
     CDS             <1..213
                     /locus_tag="PXD94_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287441.1"
                     /translation="NLTDESEEQAILAYLTENIMARIEKEQPLEKTLILYSEQLASFE
                     QKSPMALHREKFEAKPTGLAELINNL"
     gene            224..1174
                     /locus_tag="PXD94_002646"
     CDS             224..1174
                     /locus_tag="PXD94_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDL6287442.1"
                     /translation="MSLSTFAHALKLDKQGNVILFDDSPEEQAKLISRLKQNRLFKLL
                     NLVQTKHAKGEAIGLFTPIAGTTNTESGEEREPKQALAPAQKYHCEQINIDSYIRYEK
                     IDAYNLENSLEERLNQLLDDNLLRGLLMVGWQGVSRADTSNATNNQLAQDVKKGWLQK
                     IREGKPEAVINGATIGKEQQYKSLNALIKQGLGKISPAYAMGGDMIAICGRDIIADDV
                     IQLEHNDLSENLADLLTLCQQAKGGLKAVSIPYFPSNAILITRLDNLSLYLHLNNIRR
                     KAFDTPQKDSVETFYSLNVDYIVEDFNACCLIENIEVIEE"
     gene            1171..1365
                     /locus_tag="PXD94_002647"
     CDS             1171..1365
                     /locus_tag="PXD94_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287443.1"
                     /translation="MSLESYIQQAEEMELLEKLDFVSDLLELEKVSEIEAYRILTAHG
                     EPKPQPAQEKLIGCVDFLDD"
BASE COUNT          569 a          321 c          296 g          355 t
ORIGIN      
        1 aacctcacag atgaaagtga agagcaagct attctggcat atctaacaga aaatataatg
       61 gctagaattg aaaaggaaca gccacttgaa aaaacattaa ttctttattc tgaacagtta
      121 gcaagttttg aacaaaaatc gccaatggca ttacaccgtg aaaaatttga agcaaaacct
      181 accggcttag ctgaattaat taataactta taagaggtaa aaaatgagtt tatccacttt
      241 cgcccacgca ttaaaactag ataaacaagg taacgttatt ttatttgacg atagccccga
      301 agaacaggca aaactaatta gccgcttaaa acaaaatcgc ctttttaaat tattgaattt
      361 agtgcaaacc aagcacgcaa agggcgaagc gattgggcta ttcacaccga tagccggcac
      421 aaccaacacc gaaagcggag aggaacgaga gccaaaacaa gcccttgcac ccgctcaaaa
      481 ataccactgc gaacaaatca acattgatag ctatattcgc tatgaaaaaa tcgatgctta
      541 caacctcgaa aacagcttag aagaacgctt aaatcagtta cttgatgaca accttctaag
      601 agggctttta atggttggct ggcaaggcgt aagccgtgca gatacatcaa acgcgacaaa
      661 caaccaactc gcccaagacg tgaaaaaagg ctggctacaa aaaatcagag agggcaagcc
      721 cgaagcggta attaatggag caaccatagg caaagagcaa caatacaaaa gcctaaatgc
      781 tctgattaag cagggattag gcaaaattag cccagcgtat gcaatgggcg gagatatgat
      841 cgccatttgt gggcgggata ttatcgctga tgatgtaatt cagcttgaac acaacgattt
      901 aagcgaaaac ctagccgact tattaacctt atgccaacaa gctaaaggcg gactaaaagc
      961 ggtaagtatt ccctacttcc caagcaatgc gattttaatc actcgattag ataacctttc
     1021 gctatatctt catttaaaca atatccgccg taaagcgttt gatacgccac aaaaagacag
     1081 cgtagagaca ttttactccc tcaatgtgga ttacattgta gaagatttta acgcctgttg
     1141 tttaattgaa aatattgagg ttattgaaga atgagcctag aaagctatat ccaacaagcg
     1201 gaagaaatgg agcttttaga aaaactcgat tttgtttcag acctgttaga acttgagaaa
     1261 gtcagcgaaa ttgaagccta tcgcatttta accgcccacg gcgaacctaa accccaaccg
     1321 gcacaagaaa agctaatagg ttgcgtagat tttcttgatg actaacccaa gccccgaaag
     1381 gggctttttg catttttgca acaaaaaacg accgcttgca ccctttgaaa ttattgctat
     1441 cattaataaa aaacaataac caaatcaaat gagagtacag caatgacaaa ctataaacta
     1501 ccggctagaa agtggtacac cttagagcag gcaacaaaac g
//
LOCUS       DANLXQ010000269         1398 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.276, whole genome shotgun sequence.
ACCESSION   DANLXQ010000269 DANLXQ010000000
VERSION     DANLXQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.276"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..335
                     /locus_tag="PXD94_002648"
     CDS             <1..335
                     /locus_tag="PXD94_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL6287444.1"
                     /translation="VATLNYCNTEAVTRRLYYKLSSLNKIMDFSITQEDWYRSVTETQ
                     RNCVLIKNSAIEDYSINLTANKIQAKNSPIFIADPFNVLYIDDKKLLKGRDSLLKLVY
                     KTSPYLGV"
     gene            337..>1398
                     /locus_tag="PXD94_002649"
     CDS             337..>1398
                     /locus_tag="PXD94_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287445.1"
                     /translation="MKFILFDSILERHLGESLKRALESLGHQAVFTDLILHGHSMIYK
                     QADIDFMWKKVKDICDEKADMFISFRPMNLTTEMLEYIGKRMKTAIWLSDDPVLYKTC
                     YSQVVNHYDVLLHCGYKEVMDFYEANNHPKGFNFPFWTDHVAFPSVYNPLHSDYEIAF
                     LGNMNGQVRRKRYMELASLPFTKKVFGLIDSDPLAMHGGFINEAYLHTKRVTEVLSKA
                     KVGVSIPQFFTEYNGLDYDYPELAGLGYFQFPSRVIQYAASGLPIAAVGDERMKEVYP
                     EIFVGNSITELEPYIKQICEDYDFALSESAKILTRFRKNYSALSRAMMLIDLVENLDK
                     LQSQTTQERAILFTKYKAQY"
BASE COUNT          477 a          198 c          261 g          462 t
ORIGIN      
        1 tggtagcaac acttaattat tgtaatacag aagcagtaac cagaagattg tactataaat
       61 tatcatcatt gaataaaata atggactttt caattactca ggaagactgg tacagaagtg
      121 ttacagaaac tcaacgtaat tgtgttttaa ttaaaaatag tgctattgaa gattattcaa
      181 tcaatcttac ggccaataaa attcaggcta aaaattcacc tatctttata gctgatccat
      241 ttaatgtgct atatatagat gataaaaagt tattgaaagg tagagattcc ttacttaaat
      301 tggtttataa gacttctcca tatttagggg tttaatatga agtttatttt atttgattct
      361 attttagaac gtcatttggg cgaaagttta aaaagagctt tggagtcgtt agggcatcaa
      421 gcagtattta cagatttaat tttgcacggc cattctatga tttataaaca agcggacata
      481 gatttcatgt ggaaaaaagt aaaagatatt tgtgatgaaa aagcagatat gtttatctca
      541 tttcggccga tgaatctaac gacagaaatg cttgagtata taggcaagcg aatgaagacc
      601 gctatttggc tttcagatga ccctgtttta tataaaactt gctattctca agtagtaaat
      661 cattatgatg tattattaca ttgcggatat aaagaagtta tggattttta tgaagctaat
      721 aaccatccaa aaggctttaa ttttccgttt tggacagatc atgtagcatt tccaagtgta
      781 tataacccgc ttcattctga ttatgagatt gcatttttag gtaatatgaa tgggcaagtt
      841 agacgcaaaa gatacatgga attggcttca ttaccattta ccaaaaaagt attcggttta
      901 atagattcag atccattagc aatgcatggt ggatttatca atgaagcata tttacataca
      961 aaacgtgtaa cagaggtttt atctaaggca aaagttgggg tgagtatccc acaattcttt
     1021 accgaatata atggtttaga ttatgattat cctgaattgg cgggtttagg atatttccaa
     1081 tttcctagtc gagttatcca atatgctgca agtgggttac ctattgcagc ggttggtgat
     1141 gagagaatga aggaagttta tcctgaaatc tttgttggca atagtattac agagctggag
     1201 ccgtacatta aacaaatttg tgaagattat gattttgcac tatcagaatc ggctaaaatt
     1261 ctaactagat ttagaaagaa ctattctgcc ttatcccgtg ccatgatgtt gattgatttg
     1321 gtagaaaatt tagacaaact acagagccag acaacacaag agcgcgcaat tttatttact
     1381 aaatataaag ctcaatac
//
LOCUS       DANLXQ010000270         1380 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.277, whole genome shotgun sequence.
ACCESSION   DANLXQ010000270 DANLXQ010000000
VERSION     DANLXQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.277"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..451)
                     /locus_tag="PXD94_002650"
     CDS             complement(<1..451)
                     /locus_tag="PXD94_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287446.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPRFFGSE"
     gene            complement(462..1247)
                     /locus_tag="PXD94_002651"
     CDS             complement(462..1247)
                     /locus_tag="PXD94_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL6287447.1"
                     /translation="MKKYLISLEKDVKRRELFFSQPNTEDFEVFSAINTMSSDWEALN
                     NHFDLALFEQRYGRKTTKGEVGCTLSHLAVYQKIAEDSTIAEDDYCLVCEDDALFADD
                     FQKHLDALLAQNLKVDIILVGQSKIPTFNDSELELNYPSTFGFLQKNIPNSQYKYAYS
                     YLNYFAGTVAYLIKKSTACQFLAQCAGKNPYWLADDFILFEKNFKFDVLIVRPLMAIE
                     NPVLISNLEDLRGSLNNNLFKKAVKYPLKKLLAIKRNFTQRNS"
     gene            complement(1249..>1380)
                     /locus_tag="PXD94_002652"
     CDS             complement(1249..>1380)
                     /locus_tag="PXD94_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL6287448.1"
                     /translation="INILNKSNHIEVVALRVNVDNPAYIESYELLEKLGVQIVDIRE"
BASE COUNT          488 a          259 c          196 g          437 t
ORIGIN      
        1 gttccgaacc aaaaaaacgt ggcatcattg cattgctgga taataaaaaa tccaccataa
       61 acggcaaacc taataaggct gtcacaccac aaatccattg aatagcaatg ccgattttta
      121 aatcacgaac gaataaagtt ttaccttgtt ggttatgata aaaaacatta taaatcgcaa
      181 tcccgaacac aaacaaaatc agcattttga catacattgc cagcacacca atttcttttg
      241 tactgttaat caataatgga ataatgctaa agccgattaa tgcaataatt gcaatcaggc
      301 tatctttagg aataataatg ccattcgatt tgcccttgat tatttggaaa accaaatagc
      361 cacaagcaac aagcccgact acaaacgaca ttcttaaaaa atggaaaaac cacggatcgt
      421 aaagataaaa taagttaatg agtgctgaca tttcagacct attatgaatt tcgctgagta
      481 aaattacgtt taattgccag taatttttta agtggatatt ttacggcttt tttaaataaa
      541 ttattattta atgaaccacg taaatcttcc aaattactaa ttagaacagg attttctatc
      601 gccattaacg ggcggacaat taacacatca aatttgaaat tcttttcaaa taaaataaaa
      661 tcatcggcaa gccagtatgg attttttccg gcacattgtg ctaaaaactg acaagcggtc
      721 gattttttaa ttaaatatgc aaccgtacca gcaaaataat tcaggtaaga ataagcatat
      781 ttatattggc tattcggaat atttttttgc aaaaagccaa aggttgatgg gtaattaagc
      841 tctaattcgc tatcgttaaa agttggaatt ttcgactgcc caactaaaat aatatcgact
      901 tttaaatttt gtgctaataa agcatcaaga tgtttttgga aatcatcggc aaataaggca
      961 tcatcttcac agactaggca gtaatcatct tccgcaatag tgctatcttc ggcaattttt
     1021 tggtaaacgg ctaaatggct taaagtacaa cctacttcgc ccttagtggt tttacgccca
     1081 tatcgctgtt caaacagagc caaatcaaaa tggttattta atgcctccca atcactactc
     1141 attgtattaa tggcactgaa cacctcaaaa tcttccgtat taggctggct aaaaaatagc
     1201 tctcgtcttt ttacatcttt ttctaatgaa attaaatatt tcttcatttt attctctaat
     1261 atcaacaatc tgaacgccta atttttcaag caattcatag ctctcaatat aagcaggatt
     1321 atctacattc actcttaatg ctaccacttc aatatggtta cttttattta aaatattgat
//
LOCUS       DANLXQ010000271         1377 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.278, whole genome shotgun sequence.
ACCESSION   DANLXQ010000271 DANLXQ010000000
VERSION     DANLXQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.278"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>1377
                     /locus_tag="PXD94_002653"
     CDS             <1..>1377
                     /locus_tag="PXD94_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287449.1"
                     /translation="LSTEQGKKLYSRVGYDKGVWEALSVLLPERFDFEVFEVIDYSKE
                     WSWFEIIVENLFWRQPEKINMQKLHQFFKNSPYFNQATWINILYQLATEINHPLNIKY
                     LHNKLYSFSLADRDEIWTAFIALESDQCPAIERLLTWCQKSSGQENIEPEALLLGAIA
                     VSWLFTSTDKRSRNGYINALAHLLWNRLEIALALFKEFIEVNDPYVMEGILSAIHGAV
                     AYSENSSGLKALALEIDNQIFNQPETEEIYPNVLVRDYARHIIEYSLQKDEYSSDDIE
                     IIRERITPPYRSSFPQNLPTNEEIDKKYHSDGQRIIIRSMTTEYGRGMCGYGDFGRYT
                     FEAKFRNWESYKFGNLINVDLLSNYACQMIFEQFGYDDKKHLEFDKETRSRYWRRDDT
                     NKIERIGKKYQWLALYEILARVMDNFPTFSNRYADEIGLEYITNLDNFYLPKTQIKIV
                     PPTKTDVF"
BASE COUNT          472 a          197 c          259 g          449 t
ORIGIN      
        1 ggttaagcac tgaacaagga aaaaagctat atagtagagt tggctatgat aagggcgtat
       61 gggaagcatt aagtgtattg ttgccagaac gatttgattt tgaagtattt gaagtgattg
      121 attattcaaa ggaatggtct tggtttgaga ttattgtaga aaatctcttt tggaggcagc
      181 ctgaaaaaat caatatgcaa aaactacatc aatttttcaa aaatagtcca tattttaatc
      241 aagcaacttg gattaatatt ttatatcaac tggctaccga aattaaccac ccattaaata
      301 taaaatattt acataacaaa ctttattctt tttctttggc agatagagat gagatttgga
      361 cagcttttat tgctttggag tctgatcaat gcccagcaat tgaacgatta ctaacttggt
      421 gtcagaaatc aagtggtcaa gaaaatattg aacccgaagc actattattg ggtgcaattg
      481 ctgttagttg gctatttact tctaccgata aacgaagtag aaatggctat attaatgcac
      541 tggctcattt attatggaat agattggaaa ttgcattggc gttattcaag gaatttatag
      601 aggttaatga cccttatgtt atggaaggta ttttatctgc cattcacgga gctgttgctt
      661 attctgaaaa ttcatctggc ttaaaagcac ttgcattaga gatagataat caaatattta
      721 atcaacctga aacagaagaa atttatccca atgttttggt tcgtgattat gctcgccata
      781 ttattgaata ctctttgcaa aaagatgaat attcatcaga tgatattgag ataatcagag
      841 aaagaattac gccaccttat agaagttcat ttccccaaaa cttaccaacc aatgaagaaa
      901 ttgataaaaa atatcattct gatggacaaa gaatcattat ccgttcaatg acaactgaat
      961 atggtcgtgg tatgtgtgga tatggtgatt ttggcagata tacttttgaa gccaaattcc
     1021 gaaattggga gagttataaa ttcggcaatc tcatcaatgt tgatttatta agtaattatg
     1081 cttgccaaat gatttttgag caatttggct atgatgacaa gaagcacctt gagtttgata
     1141 aagaaactcg ttcaagatat tggcgaagag atgatactaa taaaattgag cgaattggca
     1201 agaaatatca atggttggct ttgtatgaaa tcttggcaag ggtgatggat aattttccta
     1261 ctttttctaa tcgctatgcc gatgaaatag ggcttgaata tattactaat ttggacaact
     1321 tttatctacc caaaacccaa attaaaattg taccgccaac taaaacagat gtatttt
//
LOCUS       DANLXQ010000272         1366 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.279, whole genome shotgun sequence.
ACCESSION   DANLXQ010000272 DANLXQ010000000
VERSION     DANLXQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.279"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1104
                     /locus_tag="PXD94_002654"
     CDS             <1..1104
                     /locus_tag="PXD94_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287450.1"
                     /translation="GHYLCTKVKGVRFFNNIAIGGRNVSAYGATSCSDIFIFGNREQG
                     MAVTTVDVEAGVQVEDVQTGECVVANNIFRHDIWMSGAKNAIINSNLCRRLRVTSGNP
                     TDTGSNNVEFSNNQAKSIAISKYGSFDNPSLFIDNINFINNILEPSEFNQDVYIDGTI
                     LKQCQFINNRCISKSKQYSVRLARNNPFLNLTFKSNKLSDNPAIISGKQGVIFCDDLS
                     LGVNKHRLRLRFSSPINFTVNQEWQKMPFDRVLNDINGHVDIVSGQFIARTGGTFHLK
                     GKITVTNSSEDVLNLYQLRLLINNEEKERLINQSVSPKNSKSNTNGMTTINFDTVVDL
                     KVGDIVDLQYKTTHELTLLSDSFLSQIYVTQEF"
     gene            1105..1347
                     /locus_tag="PXD94_002655"
     CDS             1105..1347
                     /locus_tag="PXD94_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287451.1"
                     /translation="MTKKHYCTGWRNAPANINSCCHQHDRDYGIKGTVSRAEADKRLR
                     HCLISQGNPIKAWVFWVVVRLFGWIYYKEKNINGKS"
BASE COUNT          452 a          214 c          287 g          413 t
ORIGIN      
        1 ggacattatc tctgcaccaa agttaaaggt gtgcgatttt ttaacaatat agcaattgga
       61 ggtcgcaatg tatcagctta cggtgcaaca tcttgctctg atattttcat ttttggtaat
      121 cgtgaacagg ggatggcagt gacgacagtc gatgtggaag cgggcgtaca agtggaagat
      181 gtacaaactg gcgaatgtgt ggtagcgaat aatattttca gacacgatat ttggatgtct
      241 ggtgctaaaa atgccattat caacagtaac ctctgccgtc gcttgcgtgt cacatcaggt
      301 aatccgactg acacaggcag caacaatgta gaattttcaa ataatcaagc aaaatctatt
      361 gcaatatcga aatatggttc tttcgataat ccttcattgt ttattgataa tatcaatttt
      421 attaataaca ttttggagcc cagtgaattt aatcaagatg tgtatattga cgggacaatt
      481 ttaaaacagt gccaatttat taataatcgt tgtattagca agtctaagca atattctgtc
      541 agacttgctc gcaataatcc gttcttaaat ttaacattta agagtaataa attatcggac
      601 aatcctgcga ttatatcagg taagcaaggt gttattttct gcgatgattt gagcttagga
      661 gtaaataagc acagattaag attgcgattt tcatcgccga ttaattttac tgttaatcaa
      721 gaatggcaaa aaatgccgtt tgacagagtg ttaaatgata tcaatggtca tgttgatatt
      781 gtcagtggtc aatttattgc aaggacaggc ggcacttttc atttaaaagg taaaataacg
      841 gttacaaatt catcggaaga tgtgctgaat ttatatcaac tcaggctgtt aataaataac
      901 gaagagaaag aaagactgat taatcaaagt gtttcaccta aaaacagcaa atcaaataca
      961 aatggtatga caacaatcaa ttttgatact gtagttgatt taaaagtagg tgacattgtt
     1021 gatttgcagt ataaaacaac gcatgagttg acattactgt cagactcatt tttatctcaa
     1081 atttatgtga ctcaggagtt ttaagtgact aaaaaacact actgcacagg ctggcgtaat
     1141 gcgccagcca atatcaatag ttgctgtcat cagcatgacc gagactacgg tatcaagggc
     1201 acagtcagcc gagccgaagc cgacaagcgg ttaagacatt gcttaatttc gcaagggaat
     1261 ccgattaagg cgtgggtgtt ttgggttgtg gtgaggttgt ttgggtggat ttattacaag
     1321 gagaaaaata tcaatggaaa gtcataaaac aggaaatatc attact
//
LOCUS       DANLXQ010000273         1337 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.280, whole genome shotgun sequence.
ACCESSION   DANLXQ010000273 DANLXQ010000000
VERSION     DANLXQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.280"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>1337)
                     /locus_tag="PXD94_002656"
     CDS             complement(<1..>1337)
                     /locus_tag="PXD94_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6287452.1"
                     /translation="ILLEQKNIEENRNLERMISYQLGYALINACKSFSGFISLPSELL
                     KIRKLAKDKKQQKLLPSSPVANTPKQQTALVTSAPARSQSIDLTRGLTLLDPISELCW
                     ADAFTGYPLVRKSYADHIAGSTCKFAFFESAWQANKGSWIYAFTSPGLKHNNAQALLD
                     AIQKLKDKSIPIIFWNKEDPMHYEMFKPIAEKVDYVFTTDQLVVDKYKKELGHSNIWA
                     LPFAAPIKVTNPIGRFSLDTETVCFAGTYYAQNHENRKKQMDMLLPALLAYNGCIYDR
                     ASKDTSGKYAYPEQYTHLIREGVNFNEIVKLYKKFKVFLNVNTITQSTTMMSRRVYEL
                     LASGTPVVSTPSKAITAQFPGIVITVNNEKEAELAVHKLLTDSYYWHKQSMLGIREVM
                     SKHTYENRWEFACSVINNEPIKEKTPSVRIIAVYHNYLDLAIYIDSLIHQNNV"
BASE COUNT          421 a          238 c          217 g          461 t
ORIGIN      
        1 caacattatt ttgatgaatt agagaatcta tatagatagc taaatctaga taattatgat
       61 aaaccgcgat aattcgaaca ctaggtgttt tttcctttat tggttcatta ttgattacac
      121 tacacgcaaa ttcccaacga ttttcataag tatgttttga cattacttca cgtatgccca
      181 gcatggactg cttatgccaa taatagctat ctgtcagtaa tttatggaca gcaagctcag
      241 cttctttctc attatttaca gtaataacaa tgccagggaa ttgtgccgta atagctttag
      301 atggcgtaga tacaacaggt gtgccgctag ctagaagttc atacacacgt cttgacatca
      361 ttgtggttga ttgagtgatg gtattaacat ttaaaaatac tttaaatttc ttatacaact
      421 taactatctc attgaaattt accccttctc ggataagatg agtgtattgt tcaggataag
      481 catatttgcc acttgtatct ttagatgctc tatcataaat acagccgttg taagccaata
      541 atgcaggtaa caacatatcc atctgttttt tacgattttc atgattttga gcataatatg
      601 ttccagcaaa acaaactgtt tctgtatcaa gagaaaatct gccaattgga ttggttactt
      661 taattggggc agcaaaaggc aaagcccaaa tatttgaatg cccaagttct tttttatatt
      721 tatctacaac caattgatct gttgtaaaaa cataatctac tttttctgcg attggcttaa
      781 acatctcata atgcattgga tcttctttat tccaaaaaat gataggaatg cttttatctt
      841 ttagtttctg aattgcatct aacaatgctt gagcattatt atgttttaag cctggtgaag
      901 taaaagcata gatccatgac cctttatttg cctgccaagc actttcaaaa aaagcaaatt
      961 tacacgtgct gccagcaata tgatcagcat aagattttct aactaaagga taccctgtaa
     1021 aggcatctgc ccagcataat tcagaaatag gatccagtag tgtcaatcct cttgtcaagt
     1081 caatagattg agatcgggca ggtgcacttg taactaatgc agtctgttgc tttggtgtat
     1141 ttgcaacagg ggaagatggc aataatttct gctgtttctt atccttagct aacttcctga
     1201 tttttaataa ttctgaaggc aaggatataa aaccactaaa tgatttacaa gcgtttatta
     1261 aagcatatcc taactgataa gaaatcattc tttcaagatt tctattttct tcaatgttct
     1321 tttgctctaa caaaata
//
LOCUS       DANLXQ010000274         1310 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.281, whole genome shotgun sequence.
ACCESSION   DANLXQ010000274 DANLXQ010000000
VERSION     DANLXQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.281"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>1310
                     /locus_tag="PXD94_002657"
     CDS             <1..>1310
                     /locus_tag="PXD94_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase/relaxase domain-containing protein"
                     /protein_id="HDL6287453.1"
                     /translation="VLGGFLANQLIPKSAFDWLGQYPEVISSLMYAMSGHNDKAGLLS
                     EIVQKADQHSVTLAIGGDVNKLVQRPVNSLPKQIVLALRYLLEQKIKLNTPKGPADGW
                     FTEDGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQSLGIVEESPSGGAIWSCR
                     IQADSGWTPATTFTLLRVKPEVAWENINDRPAYFAGKVKVIEQITEEVNAEVQPPLVE
                     ETTKISLLPSKQSEAIIPSEETEISKAITSSDNATSAEVIINNENDLTSFVLDMFAPT
                     ETIEQNISVISSNKEQQIVMTTLTEQEKVPPKKVKLEKSKKKNLQLTTLEPWNDMINP
                     ITGKEFVNWLKEGIATNKFAINKPSAKLHIVDGNLFLVTPVIFEMYLQECGQPYDKET
                     INNLQYDFQGLGLHSLKKTMTNGKPDTINFWKCDVIGPRKTSSL"
BASE COUNT          433 a          237 c          282 g          358 t
ORIGIN      
        1 ccgtgctagg aggattcctt gcaaatcagc tcattcccaa gtctgcgttt gattggctgg
       61 gacagtatcc agaagtcatt tcttcactga tgtatgcaat gtctgggcat aacgataaag
      121 ccgggctttt atctgagatc gtgcagaaag ccgatcagca tagtgttacg ctagcaatcg
      181 gtggcgatgt gaataagctg gtacagcgac cagttaattc attgccgaag caaattgtgc
      241 tggctctacg ttatttactg gagcagaaaa tcaaattaaa tacgccaaaa ggaccagcag
      301 acggctggtt taccgaagat gggctgtggc tggtaagtaa aactgctgcc gataatatcc
      361 gaacttattt actgggacag gggatttccg tgcctaaaga taatccaaaa ctgtttgatg
      421 aaatgcaatc tcttggtatt gtggaagagt ctccttccgg cggtgcaatt tggagctgcc
      481 gtatccaagc cgattccggt tggacaccgg cgacaacatt tacgctatta cgagttaaac
      541 cggaggttgc ttgggagaat atcaacgata gaccggcgta ttttgccgga aaagtcaaag
      601 tgattgaaca gataactgaa gaggtcaatg cagaggttca gcctccatta gttgaagaaa
      661 ccactaaaat ttctctatta ccgagcaagc aatcggaggc tattattccg tctgaggaaa
      721 cggaaatatc gaaggcaatt acaagttcag acaatgcaac atctgcggaa gtaatcataa
      781 ataatgaaaa tgatttaact tcttttgtat tggatatgtt tgctcctaca gagacaattg
      841 agcaaaatat ttctgtcatt tcttcaaata aagagcagca aatcgtaatg acaacactta
      901 cggaacaaga aaaagttccg ccgaaaaaag ttaaattaga gaaaagtaag aaaaaaaatc
      961 ttcaattaac cactctagaa ccatggaatg atatgattaa tccaattact ggaaaagaat
     1021 tcgtaaattg gttgaaggag gggatagcaa ccaataaatt tgcaattaac aaaccttctg
     1081 caaaattaca tatagtagat ggtaatttat ttttagtcac accagttatt tttgaaatgt
     1141 atttgcaaga atgtggccaa ccttatgata aggaaactat aaacaatctt cagtatgatt
     1201 tccaggggct tggtcttcat tctcttaaaa aaaccatgac taatggtaag ccagatacaa
     1261 taaatttttg gaagtgtgac gtgattggtc ctagaaagac ttcctcttta
//
LOCUS       DANLXQ010000275         1306 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.282, whole genome shotgun sequence.
ACCESSION   DANLXQ010000275 DANLXQ010000000
VERSION     DANLXQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.282"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..1067
                     /locus_tag="PXD94_002658"
     CDS             <1..1067
                     /locus_tag="PXD94_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287454.1"
                     /translation="FNKKLILTFAATLVLSACGGSGSGGSSSTPNHPKPVLVPKTQNN
                     LQAQNVPQAQNASQAQNAPQAQNAPQAQNAPQVENAPQAQNAPQVENAPQAEVTPPVP
                     QPQSQKIDGSFDKIGSVKLNKEAQTLELSRFTLVDKLGTPPKFDKVSGKKIIEEKDFL
                     VLNLSDINAEQLSGDFLIRRSDDLFYGYYHDTNGKNLVDAADKFSQYFVVYDEKRVND
                     NISDKLTATYRKKEGFVYGSNPHTKEFAARISKLGDVEIKFENGQAQGSIKDEKDGNA
                     EIFTIKGDTKQLEITPTESNRIIIAILDQNQKSYTPGMEKAIMETKFIDSKAGNSDQK
                     YLIGEAKSDNWQAIMVSEKK"
BASE COUNT          468 a          248 c          237 g          353 t
ORIGIN      
        1 aattcaataa aaaattaatt ttaacatttg ctgcaacctt agttttaagt gcttgcggag
       61 gaagcggtag cggaggttcg tcttcaacac cgaatcaccc caaaccagta ctagtaccaa
      121 aaacacaaaa taatcttcaa gcacaaaatg ttcctcaggc acaaaatgcc tctcaggcac
      181 aaaatgcccc tcaggcacaa aatgctcctc aggcacaaaa tgctcctcag gtggaaaatg
      241 ctcctcaggc acaaaatgct cctcaggtag aaaatgctcc tcaagcagag gttactccgc
      301 ctgtaccaca gccacaatca caaaaaattg acggttcttt tgataaaatt ggttcagtaa
      361 aactcaataa agaggctcaa actcttgagc ttagtagatt cactttggtg gataaattag
      421 gcacaccacc gaagtttgat aaagtaagcg gtaaaaaaat tattgaagaa aaagattttc
      481 tcgtattaaa tttgtctgat attaatgctg aacaactctc tggcgatttt cttattcgcc
      541 gtagcgatga tctattctat ggctactatc acgatacaaa tggcaaaaat cttgtcgatg
      601 ctgccgataa attcagtcaa tattttgtcg tgtatgatga gaaacgggta aatgataata
      661 tctctgataa attaacagca acttaccgta aaaaagaagg ctttgtatat ggttcaaatc
      721 cacatactaa agaatttgcc gcacggatca gcaaattggg ggatgtagaa attaaatttg
      781 aaaatggtca agctcaagga agtataaaag acgaaaaaga tggaaatgct gagatcttta
      841 ctattaaagg tgatacaaaa cagttagaga ttaccccaac ggaaagtaac cgaatcatta
      901 tagcaatttt agaccaaaat caaaaaagct atactccagg aatggaaaaa gcaattatgg
      961 aaactaagtt tattgattca aaggctggta attccgacca aaaatactta atcggtgaag
     1021 caaaaagcga taactggcaa gcaataatgg ttagcgagaa aaaataaagt tatcttttgc
     1081 taaaaactga aataaaaagg ctgagtccgg gtaatatcgg cctcagtctt ttaaattgta
     1141 gaaaatcatc tgtagaagat caaacctaat ctgacagtcc cccgttttaa attaccgtgt
     1201 ctgtcagatt aatttgagct taaattcttt tccacccaaa tccgttttcc atcaagtaag
     1261 gttgccatcg gtgttctgcc atagcacatt tttccttgat gtgttc
//
LOCUS       DANLXQ010000276         1283 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.283, whole genome shotgun sequence.
ACCESSION   DANLXQ010000276 DANLXQ010000000
VERSION     DANLXQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.283"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            159..434
                     /locus_tag="PXD94_002659"
     CDS             159..434
                     /locus_tag="PXD94_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6287455.1"
                     /translation="MAKATVARRIKNAINGNFGDHKSLAGTGGLYEMRIATGAGYRIY
                     YAQQGNVIYILLHGGDKSTQQADIEKAKALWAELKTTGESNGNNTNQ"
     gene            412..735
                     /locus_tag="PXD94_002660"
     CDS             412..735
                     /locus_tag="PXD94_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL6287456.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNNVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
BASE COUNT          451 a          242 c          241 g          349 t
ORIGIN      
        1 ctgctctctt ttcaaaattt aaccacttga ttatttgtat cttaatggat acactaagca
       61 ataaatagaa taagaggctg gcaatgacag agcaaaccac taatcaaatc atcattaccg
      121 ataatttttc aaaatagctt gagaatttaa agaaccctat cgcaaaagca acggtagcaa
      181 ggcggataaa aaatgcgatt aatggcaact ttggcgatca taaatcttta gccggtacag
      241 gcggacttta tgaaatgcgg atagcaacag gagccggata tagaatttat tacgcacaac
      301 aaggaaatgt aatttatatc ttgttacacg gcggcgataa atcaacacag caagccgata
      361 tagagaaagc gaaagcatta tgggctgaat taaaaactac aggggaaagc aatggaaaca
      421 atacaaatca atgaacaggc agcagaaaag cacggtttac gcccttttga cgtggcggag
      481 tttttaaccg atgaggcaac tattcaagcc tatttagcgg aagtgttaaa agagggcgac
      541 caagacgaat ttttagaagc cttgaacaat gtggcaaggg caaggggaat ggcggagctt
      601 gccaagcaaa cagggctagg gagagagagc ctatataaaa ccctagccaa aggcagcaaa
      661 ccacgctttg acactattca aaaaattcta ggggcattca atttagtgct tattccacaa
      721 gtcaaaaatg gctaaaactt atcacttgct acctgccaaa acctcacatt caaatagccg
      781 ttgattgata ttaacggctt ttttattgct taattctgta ccaattacgc accgattgcg
      841 gatcaatacg gggcaagttt tcgcaaaacc ttaatgaaat atgaccgctt gtgattttgt
      901 acaaaacgac acaaaccaat gtataaaatc aaaacttgcc acaaatggcg gaatgatatt
      961 aaatgcccca caaactagca gtatttctta atgacttttc attttaaggg tacatttaag
     1021 ggtatgttta acaagtctta aatgcacttc tcaataataa caagcctcat agcatacaat
     1081 tcaaactcgg ctggggacca tttaagcgtt gcatattgtc tttcactgtc tcaaatttac
     1141 tataaaatca agaacttaga ttattttata gtctcttatt gtcttcttta gttgcattgt
     1201 atcttagcgt ttatgatccc taaaatgatc cctattttga aaacgggaaa atttagggat
     1261 cataaacttc gataaaccct tat
//
LOCUS       DANLXQ010000277         1265 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.284, whole genome shotgun sequence.
ACCESSION   DANLXQ010000277 DANLXQ010000000
VERSION     DANLXQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.284"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(144..>1265)
                     /locus_tag="PXD94_002661"
     CDS             complement(144..>1265)
                     /locus_tag="PXD94_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL6287457.1"
                     /translation="QMNSELGDQQARVPIWWGLFHQINKGVTHALTASIPCKPDLRQV
                     RFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQNSQLSLSPAQVRETSWLGGRILIA
                     NSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANNGLKDRLLDDVK
                     QNFSAKVIDFFSSKENSDESILRTVLRAENMDISRGKVYPGYGGNLDRSFTNEVTKTA
                     SMFGLSLGSIGAFPAFDSMRQALPMVHSFALMALIILTPLIMVMSGYSLKTLVTLTFV
                     NFALVTLTFWWELARWLDSWLLSVLYDSGTHSRINPYFLENTQDDIIVNFVMGSMFLV
                     LPAIWLGSMSWVGVKMGEFASHLTTGSRDSKTAGSKIGK"
BASE COUNT          394 a          266 c          231 g          374 t
ORIGIN      
        1 cacttttttc tgatgatgaa gaacaagaag atgatgaata tcaatatggg tatgatggac
       61 caggtatata tctaggtaat taccgtgttg atgagtaaaa gtaaatagag tggtatatac
      121 cactctatta ttcagttgtt agattatttc cctatcttgg aacccgctgt tttagagtct
      181 ctagaaccag tagttaaatg acttgcaaac tctcccattt ttactcctac ccaagacata
      241 ctgcctagcc aaatcgctgg taagaccaag aacatagaac ccatcacaaa attcacaata
      301 atatcgtctt gtgtattttc aaggaaataa gggtttattc tgctatgtgt gcctgaatca
      361 tagaggaccg acagcaacca agagtcaagc catctagcta gttcccacca aaatgtgagt
      421 gtaactaatg cgaaattcac aaaagttagc gtaaccaatg tttttaacga ataaccactc
      481 ataaccataa ttaaaggcgt gagaataatc aatgccatta atgcaaatga atgcaccatc
      541 ggtaatgctt gccgcatcga atcaaaagca gggaatgctc cgatactgcc taatgacaga
      601 ccaaacattg aagctgtttt agtcacttca tttgtaaatg atctgtctaa atttccccca
      661 tacccgggat aaactttccc tctcgaaata tccatattct cagctcgtaa tacggtacga
      721 agtatggatt catcggaatt ttctttactg ctgaaaaaat caatcacttt tgcagagaag
      781 ttctgtttta catcatctaa taaacgatct tttaaaccat tattcgccgt atcccaccac
      841 tctgcacaat ttggaaaacc accctctcca ttattcggca atccattatc ccgagaagca
      901 ttatagggaa atagactatg aggttgttgg gctcgataac ggggataaag ctcgctattt
      961 gcaatcaaaa tacggccacc taaccagcta gtctcacgaa cctgtgctgg gctcaatgaa
     1021 agttggctat tttgaatttt ctgacgtgcc ggtacatagc attgttgtac aaataagcga
     1081 acctctgtca ataatgctgg attattgatt ttctcgtgtt gaacttcaaa acgaacttga
     1141 cgtaaatccg gtttgcaggg aattgaagct gttaaggcgt gtgttacccc tttgttgatt
     1201 tgatgaaata atccccacca gatagggact ctcgcttgtt gatcacccaa ttcagagttc
     1261 atttg
//
LOCUS       DANLXQ010000278         1258 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.285, whole genome shotgun sequence.
ACCESSION   DANLXQ010000278 DANLXQ010000000
VERSION     DANLXQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.285"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            7..1236
                     /locus_tag="PXD94_002662"
     CDS             7..1236
                     /locus_tag="PXD94_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL6287458.1"
                     /translation="MQFREINPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSL
                     MNETMEMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNE
                     ILARSGADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKI
                     CKQYDAMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEII
                     AKRKRVEKGIRAESDVDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTETTSAQYGVHEA
                     LRIILQEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPA
                     LRSDILNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVP
                     SGDAVAAALAVYKEADK"
BASE COUNT          387 a          259 c          266 g          346 t
ORIGIN      
        1 ataattatgc aattcagaga aattaatccc tcccctcgtt tattaatgtg ccctggccca
       61 acagatgctg acccacgtgt tttgcgtgca atgtctgcac ccattttagg gcaatttgac
      121 cctgagttta cctcattaat gaatgaaaca atggaaatgg aacgccgttt attccaaacc
      181 aaaaatgagc aaacttatgt agtcaactcc acctctcgtg gtggcttaga aactgttcta
      241 accgcagcga tttgcccagg cgataaagtg ttaattcctg catttggtcg ttttggttat
      301 ttattaaatg aaattttagc tcgtagcggt gcagatatta ccattatcga aagagagtgg
      361 ggaaccgtat ttgaaccaga agaaattgaa gcagagttga aaaaaggcgg ctataaagca
      421 gttgctatta ttcacggcga aacctcaacc tctatgatgc aaccgttaga agagatcggc
      481 aaaatctgta aacaatatga tgcaatgtta attgtagata ccgttgcaac cttaggcggt
      541 gtagatattc gtgtggatga atggggcatt gatgcttgta tcggcggtac acaaaaatgt
      601 atttccgccc catcaggcac agctttaatc acttataaca aacggattga agagattatt
      661 gcaaaacgta aacgtgtgga aaaaggcatt cgtgcagagt ctgatgtaga tggcacatta
      721 ccgccaattc caagtaacta cttagacttg gcacagctac aagattactg gagcccacgc
      781 cgtttaaatc accacacaga aaccacttct gcacaatatg gtgtacacga agcgttacgc
      841 attattttac aagaaggctt agaagctcgt tttgctcgcc atattttaaa cgacaaagca
      901 ttatgtgcag gtttagaagc aatgggtttg aaaatttttg gcgatcgcaa acataaagcc
      961 cccgttgtaa cggcattcca cattccagaa ggtatgagtg gcccagcatt gcgtagtgat
     1021 attttaaacc acttcggtat tgaattagcc acctcattcg gccccttaga tggaaaagta
     1081 atccgtatcg gtaatatggg tttctcaagc caaaaacgta acgtactttt aaccttaggt
     1141 gcgttagaag ccgtactttt aagccataaa gtaaaagtgc cttcaggcga tgcggttgca
     1201 gctgccttgg cagtctataa agaggctgat aaataaccac cttcctaaat ttacaata
//
LOCUS       DANLXQ010000279         1246 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.286, whole genome shotgun sequence.
ACCESSION   DANLXQ010000279 DANLXQ010000000
VERSION     DANLXQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.286"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            229..474
                     /locus_tag="PXD94_002663"
     CDS             229..474
                     /locus_tag="PXD94_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287459.1"
                     /translation="MTNDGSIIARGSGFTTYALELENDGLKYLVGFVQPNGNAKEFIE
                     PGATIRRSNDQPSIGSVDCDPRKKIHYKFDVHLMNTL"
     gene            693..>1246
                     /locus_tag="PXD94_002664"
     CDS             693..>1246
                     /locus_tag="PXD94_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4852 domain-containing protein"
                     /protein_id="HDL6287460.1"
                     /translation="MCKKAFPLWGLIFGLLLSTSAFSVSKNFNYQNILMESLHFSENI
                     ELSSLSKDWLLYSDRNTYHKYQNDEFEFQDKIEQAGKKLQKLVDDRKSEEAPIVFTIP
                     VNSEFGNYDFEQGKFEYQPLMNGHMYISSNQYTENLPRKINLYVSNPEFIDGIPMGKD
                     KAKEFLQKRKNSLGNINRSVILYV"
BASE COUNT          423 a          202 c          229 g          392 t
ORIGIN      
        1 gtactggtaa tggctgttaa attctaaata gctacaattt tcaataaaac tgtagctatt
       61 ttttattaag aatttatttt agtattaatt ttatattgca ccacactatt tcccatatct
      121 ccgactttta ccagcacaga aggtaaacct ctcacagtta aacgggtagg aaatgattac
      181 gagtattcgt atgacaaaac tactttcaaa aacccgatta aaaaggcagt gaccaacgac
      241 ggttcgatta tcgccagagg ctcaggtttt actacttatg ccttagagtt ggaaaatgat
      301 ggcttgaaat atctagtcgg atttgttcaa ccaaatggaa atgcaaaaga gtttattgag
      361 ccgggagcaa caattcgccg tagtaatgat caaccaagta ttggttctgt ggattgtgac
      421 cctcgtaaaa aaatccatta taagtttgat gttcatttga tgaatacttt ataatacctt
      481 tgtggtgcta gagctagccc aagttaaaaa agacaaagct cggcagatga acaattgtgg
      541 tgtcagagct gacccacgtt aaaaaacaca atgctcggta ggtaaatccc ttaggtttaa
      601 aaaatctaag ggatttttgt ttaaaagact tgctctttat tatcaggtgg atctatcata
      661 atttaaactc tctaatgtgt caggagataa aaatgtgtaa aaaagctttc ccattatggg
      721 gcttaatctt cggattactg ctatccactt cagcattctc agtgtctaaa aacttcaact
      781 atcaaaacat attgatggaa tcattacatt ttagtgagaa tatcgagctt agttcgttat
      841 ctaaagactg gttactttac agcgatagaa atacgtatca taaatatcaa aatgacgagt
      901 ttgagtttca ggataaaatt gagcaagctg gtaagaaact acaaaaactg gtggatgaca
      961 gaaaatctga agaagcccct attgttttca caattcctgt aaactcagaa ttcgggaatt
     1021 atgattttga acaaggtaag tttgagtacc aacccctgat gaatgggcat atgtatattt
     1081 cttctaatca atatactgaa aatttgccaa gaaaaattaa tctgtatgtt tctaatcccg
     1141 agtttattga cggtatacct atgggcaagg ataaagctaa agagttttta cagaagcgta
     1201 aaaattcttt gggaaacata aatcgctctg ttatattgta tgttga
//
LOCUS       DANLXQ010000280         1211 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.287, whole genome shotgun sequence.
ACCESSION   DANLXQ010000280 DANLXQ010000000
VERSION     DANLXQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.287"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..230
                     /locus_tag="PXD94_002665"
     CDS             <1..230
                     /locus_tag="PXD94_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDL6287461.1"
                     /translation="LSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIYSYISPKT
                     GKRSTKNRIGSYPQMSLVEQSPQVFEQIARL"
     gene            282..869
                     /locus_tag="PXD94_002666"
     CDS             282..869
                     /locus_tag="PXD94_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL6287462.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLST
                     LLTKLGKTPSEIESILEQQAEKGTMDNLFKGLGNA"
     gene            862..1211
                     /locus_tag="PXD94_002667"
                     /pseudo
     CDS             862..1211
                     /locus_tag="PXD94_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252950.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
BASE COUNT          444 a          231 c          240 g          296 t
ORIGIN      
        1 cactttcaga cacattacta aaaaatctta aatattcccc aaatgcgaag cctatcgcag
       61 acagtaacgg gctatttatt cttgcacaga aaaaagcgaa agtatggatt tatagctata
      121 tcagccccaa aacaggcaag cgaagcacaa aaaatagaat cggtagctat ccgcaaatga
      181 gcttagtgga acaatcccca caggtatttg aacaaatcgc acgtctttaa aatattaaat
      241 taagagaaag cactatttgc gatgtagcag gagataaaac aatgtttgat gaattaaaaa
      301 tagcccaagc gaccgcctat ttgctacata aggcaggcgg aacaatggag catattaagg
      361 taatgaaatt gctttatctt gcggatcgct tatcgtggca aagatacggc tattctattt
      421 ctaatgatga atattattca ttgccttatg gaccggtatt aagcaacaca ctcaacctaa
      481 tgcgaggaga agaagcgagc ttataccaaa aaaccagtat ttggggcgag tggatagcag
      541 ataaggaaga tcacaaaatc agccttaaaa aatccgtaaa taccgaagat gaatacttct
      601 ttgattgcct ttcagagagc gacaccgaaa ttttagaaga ggtatttgcg acctttggaa
      661 attataagcc attcgcccta gtggaatata cccataaccc taaatacatt cccgaatggg
      721 aagacccaca aggcagaagc aagccgattt ctttaagcac gcttcttact aagctaggga
      781 aaacgccaag cgagatcgaa agcattttag agcaacaggc ggaaaaaggg acaatggata
      841 accttttcaa gggcttaggc aatgcttgaa atcaaaagac aagtaacgat tcttagaccg
      901 accaactacg gcaaggaaga gaaccactac aagcatttat atattctgct taccgatccg
      961 tgctttgatg gtgaaaccgg taaaggagat atgattttaa gtgtaaactg ctcaagcatt
     1021 aaagacggga aagcacttga tgaaacttgt attttaaagg caggaacaca cgaatttatc
     1081 acacgggaca gcttcatttt ctaccgccat ataaaaattg attcgttaga tgagattaag
     1141 gcaaatatag aagccggtta ttttattcaa aagccctaat taatgatgaa gcctatcaac
     1201 aaatactaaa a
//
LOCUS       DANLXQ010000281         1206 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.288, whole genome shotgun sequence.
ACCESSION   DANLXQ010000281 DANLXQ010000000
VERSION     DANLXQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.288"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(66..1088)
                     /locus_tag="PXD94_002668"
     CDS             complement(66..1088)
                     /locus_tag="PXD94_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL6287463.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPNKLV
                     IRDSAA"
BASE COUNT          364 a          259 c          199 g          384 t
ORIGIN      
        1 ttaaattcta tcattttcta caagacttgc cacgatcttt tatagatttt tatgcaaaat
       61 caatattatg cggcgctatc acgaatcact agcttattcg gtaatttaac aatcatctct
      121 tctgctgata aggttttacc actcaaaaga gtaagtagtt gtttacctgc tatttttccc
      181 atttgctcgt aagggatcgc aatggttgtc aatttagggt aggaaatact ccccacttct
      241 aaaccaccta aaccggcaat cgcaagatct tttggaatgc tcatatgtcg gcgtaatgct
      301 tcatataatg ctccgcaagc caattcatcg gataaaaaaa ccaatgcatc tattgcttcc
      361 caatttagaa gggcttcagt caacaaagtt gctcctgccg aaaacgaaat atcctctact
      421 acgtggtgga tttttaaagg agaaatatga tgtgcaaata aagtcgtatg ccaactttct
      481 aaatattgtt tgaagatgga taaatcttgc ttcgccgata acaatccaat ctctcgatag
      541 ccttttttca ccaaaaaatg gatcattttc tccattgctt cgtggctatc aattccaaca
      601 ctgagatgaa tcggttcttt ttttatcgca cctatttcta cggtagggat attgctgttt
      661 ttaatccact cacggacatt aggagggcaa tctaaattca aaaacactat cgcttgagga
      721 gcatgattaa tcagttcttg aaaccaagat tcttgatcag cgatcaaaat caccatttgc
      781 aatgcggaat ccgccacgct tttgcgtaat gccgataaaa ttaaggtatt ttccgttgag
      841 ctaatcgacg tcgtcacaat gaccaaatta tgtgattctg cagaagctaa ttgccgagca
      901 gaaaaatgcg gaatatatcc tagttcaatg attgcctctt caatccgttt acgtagtggc
      961 tccgacacca tttctggtgt acgcaaagca cgagataccg tcatctgccc aacaccaaca
     1021 actttggcta catcagctaa agtgactctg cccgaagatc ttcgatttct acttgttcca
     1081 tttgccatac tattttccta attgttccat attatagggc taaatcctta attttcctat
     1141 ttagaaagaa cagagcaaaa tgccaactga ttatttttta tctaattaaa tactaaaaat
     1201 gttaac
//
LOCUS       DANLXQ010000282         1177 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.289, whole genome shotgun sequence.
ACCESSION   DANLXQ010000282 DANLXQ010000000
VERSION     DANLXQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.289"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            78..959
                     /locus_tag="PXD94_002669"
                     /pseudo
     CDS             78..959
                     /locus_tag="PXD94_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          415 a          210 c          217 g          335 t
ORIGIN      
        1 gtcattattt ttataacatt tctattggaa gtatgtctgg aacaggtatt ccaagaataa
       61 cattaactaa attagaatct tatttagtcc cagttccgcc actcctagaa caacagcgca
      121 ttgtgcaaaa aatcgaggaa ttattaccgc ttgttgaacg ctacgagcaa accgaacagc
      181 aactcactaa actcaacaat acattccccg aacagctgaa aaaatcggtg ttacacgcag
      241 ctattcaggg taaattaacc gagcaagatc cgaatgatga actggcatct tgcctgattg
      301 agcggataaa agcggagaaa aatcgtctta ttgccgagaa aaaactcaaa aaaccaaaaa
      361 gtgtttcaga gattgtaatg cgtgataatt taccttatga gatcaaagca ggtcaggaac
      421 ggtgtatcgc cgatgaagtg ccatttgaga ttcctcaaaa ttggacttgg gtgaggttgg
      481 gggaagtagg tgattggagt tcaggtgcaa ctccaaaccg acacaatcct gaatactata
      541 atggtacgat cccgtggcta aaaacaggag acttaaacaa cggaataatt tcatctatac
      601 ctgaatttat tacagataaa gcattgcagg aatgttctgt aagattaaat cctgttggtt
      661 cagttttgat agcaatgtat ggagcgacaa ttggtaagct tgggatatta aaaatagcag
      721 caacaacaaa tcaggcttgc tgtgcgtgta ttccttttaa tggaatttat aataaattct
      781 tattttatta tttgatgtcg cagaaagcag aatttcagaa aaaaagtgag ggaagtgggc
      841 aaccaaatat atcaaaggaa aaaattatca attatttatt tcctttacca ccaatccacg
      901 aacaacatcg cattgttcaa aaaattgaac aattatttgc agagattgaa aaattgtagg
      961 agtcatcatc gctttcttgg gcgtgttgga tacgccctac agtcactaaa ttattgatct
     1021 tttatcatat cggttttcaa ccaacctaaa aggaaaccta tatgatccat ctatttgaaa
     1081 agcaccttaa tcaatctaat cttgctgaaa atacccaatc agcttatctt tttgcggtaa
     1141 aacaatatca caaaaaattt gatcagttat ctaaaat
//
LOCUS       DANLXQ010000283         1137 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.290, whole genome shotgun sequence.
ACCESSION   DANLXQ010000283 DANLXQ010000000
VERSION     DANLXQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.290"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            47..325
                     /locus_tag="PXD94_002670"
     CDS             47..325
                     /locus_tag="PXD94_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL6287464.1"
                     /translation="MSVLNDVKKTAQRDWHKADILAELRKKGWSLRSLAKAGNVSHNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            370..>1137
                     /locus_tag="PXD94_002671"
     CDS             370..>1137
                     /locus_tag="PXD94_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6287465.1"
                     /translation="MSELSLKTHYSVAELLSFKLSSLPSAHKNVLEKAIRENWQSQKR
                     KGRGGGVEYELISLPTEVQQEIRTKLLKLLPAEISKGELSVVRQNIDLEQITDKQLST
                     ADARIMVVRWFLMQEVQLGLSRTKTLDQVIAAVASSEIPAEICKAIMAGNSKAGGKLK
                     LSKRTLHSWVLAYEAGENSAERLKQMIPLKTQKRAVPERCGWLQAFLPFYQTFSNVAL
                     TQAYAQFAMQYEGEDLPTESQVRYALKQLPDYVVQQGR"
BASE COUNT          365 a          216 c          281 g          275 t
ORIGIN      
        1 ttacggttaa atacacgcat caaatagata aaaagaggtg tatcgaatga gtgtattaaa
       61 cgatgttaaa aaaaccgcac agagagattg gcataaagcc gatattcttg cggaacttcg
      121 taaaaaaggt tggtcacttc gttctttggc taaagcaggc aatgttagcc ataacacgct
      181 gaagtctgtt ttagacaagc cttaccccaa aatggaacgc ttagttgcaa atgccatagg
      241 ggttgcccct gaagtgattt gggctgggcg aacagcagaa cgcattgaac gtaatcggcg
      301 accaacttta actaataagt tttaatcata aatgatcttt aacgtaaaag atataggaga
      361 agaacattta tgagcgaatt aagtttaaaa acgcactatt cagtggctga attgttaagt
      421 tttaagttat ctagcctacc aagtgctcat aaaaatgtgc ttgagaaagc aataagggaa
      481 aactggcaat ctcaaaaacg caaaggcaga ggcggcgggg ttgaatatga attaatcagt
      541 ttacccaccg aagtccaaca ggaaatccgc accaagttac tcaaacttct tcctgctgaa
      601 atcagcaaag gcgaattgag tgtagtgcga caaaacattg atttggagca gataaccgat
      661 aaacaacttt ctacggcaga tgctcgcatt atggtggtgc ggtggttctt gatgcaggaa
      721 gtgcagttgg ggctaagccg cacaaaaacg ctagaccaag tcattgcagc ggtggcaagc
      781 agtgaaattc ctgctgaaat ttgcaaagcg attatggcgg gcaatagtaa agccggtgga
      841 aagttaaagc tatctaaacg caccttgcat agttgggtgc tggcgtatga ggcaggcgaa
      901 aattcagcgg aacggttaaa acagatgata ccgctgaaaa cacaaaaacg tgccgtgccc
      961 gagcggtgtg gttggttgca agcctttctg ccgttttatc aaacctttag caatgtggca
     1021 ctcacacagg cttatgctca atttgcaatg cagtatgagg gcgaagattt acctacagag
     1081 agccaagttc ggtatgcctt gaagcagttg cccgattatg tggtgcaaca agggcgg
//
LOCUS       DANLXQ010000284         1093 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.291, whole genome shotgun sequence.
ACCESSION   DANLXQ010000284 DANLXQ010000000
VERSION     DANLXQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.291"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..192
                     /locus_tag="PXD94_002672"
     CDS             <1..192
                     /locus_tag="PXD94_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287466.1"
                     /translation="PQDGSVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSESYT
                     VDGESVRDIQLNALRKVNE"
     gene            192..560
                     /locus_tag="PXD94_002673"
     CDS             192..560
                     /locus_tag="PXD94_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287467.1"
                     /translation="MKITLNLGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLATEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            590..778
                     /locus_tag="PXD94_002674"
     CDS             590..778
                     /locus_tag="PXD94_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287468.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            831..>1093
                     /locus_tag="PXD94_002675"
     CDS             831..>1093
                     /locus_tag="PXD94_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287469.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTAEKAFKNTERAAQKSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVR"
BASE COUNT          348 a          216 c          274 g          255 t
ORIGIN      
        1 cctcaagacg gttcggtaat tgattgggat aacattaaaa atgcgaagct cactaaatac
       61 ccgttagaca atagcgaaaa acgcacctct tatttaggct gttttactac cgatgtgagc
      121 gagtcttaca ccgttgatgg tgagtcggtg cgtgatattc aactcaacgc attgcgtaag
      181 gtgaatgaat aatgaaaatc actttaaatc ttggcattga atataacggg aaaattcgcc
      241 gtgattgtac ggtgcgattg ctgacgatgg gcggtcaatg cacggctcaa gaaatgattg
      301 cggatttagg gtttgaggaa aactcggaag ccctatcaaa gcgtgaacaa accttggtcg
      361 atatggctta cctttcgcag caagttacct ttgatggtat ttatcaaggt aatgtgacgg
      421 ctcaattttt atttgacaat ttagctaccg aagactactg gcagttgatt gaggcaacgc
      481 tcacactccg aaaaaagcgg ttagaaaatg gggtgagcca aagcgagagc gaaagcccgg
      541 caaatcccga tttggcgtag cagaagcctt taagcagtac cgacaagcgg tgattttact
      601 ctcaaaattt gcaattaccg ccgagcaggt atggcaaatg ccgttgcttg aggtatttgc
      661 gtggattgag agctacttgg agagtgaggg cgtaaaaaca ccgcatagcc caatgggtgc
      721 gaatagcgaa gtgaaacgcg aaagctatgt ctttactcgg tgtggcaaaa ttgaataggg
      781 gcgaaagccc cttttttgta ctttaaaatg agtttaaaga gactttaaaa atggcaaaaa
      841 atatgaaagt gcaactggaa ttgacggcaa aagatactgc ctccccagtt ttaaaccaag
      901 ccgctaaaac cgccgaaaaa gcctttaaaa atacagagcg agcggcacaa aaaagtggca
      961 atgctcaaac gcaagcagca cagaaagcag cgaatgctac agaaagctca aatcggaaaa
     1021 tagaacagag ttaccgtaat gcccgccgag tggctgccga tgtgagccga gcccgtgaaa
     1081 cattaggtgt tcg
//
LOCUS       DANLXQ010000285         1084 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.292, whole genome shotgun sequence.
ACCESSION   DANLXQ010000285 DANLXQ010000000
VERSION     DANLXQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.292"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            85..>1084
                     /locus_tag="PXD94_002676"
     CDS             85..>1084
                     /locus_tag="PXD94_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDL6287470.1"
                     /translation="MADSVKSHEQSSDEIDLIEIFRALWDKKWWIVITTFITTLLAGI
                     YAFTAKEQWTSKATVIAPKLTELGEYLNVRREYSRILRVDPVDPNALSASLFNNFNRL
                     AQSQDTKNEFFITSNVYKTLAEGKDEQGQRTVLSNLSNENTSFIIPDAKKDPNAIGRT
                     LSFSAETPMEAQDTLGKLVQFINSKAFAVELEDFLVDFQNVLTALQYEKSLIQEDLSV
                     NKSVQLENLNKAYETAQKAGIKEYSKAFNQSDASNIAAALSDTKISLSDSKLADSSYL
                     FMLGEKYLKAQIDVANEKGVVYPPRYYQIDYQLKQLEPLFEKTKTVKTQTFSYQASP"
BASE COUNT          388 a          166 c          202 g          328 t
ORIGIN      
        1 cttaaaaaag agtaaatgaa tgcagtggaa ataagaaaat tatgtataat gctgtacaga
       61 aacatgcaaa ttttagagga aactatggca gattcagtaa aaagtcatga gcaaagctcg
      121 gatgagattg atttaattga gatttttcgt gctctatggg ataaaaaatg gtggattgtt
      181 ataacaacat tcattaccac attacttgct ggtatttatg catttactgc aaaagagcaa
      241 tggacgtcta aggcaacagt tattgcccct aaattaacag agttaggtga gtatttaaat
      301 gttcgcagag aatatagtcg gattttgcgt gtagatcctg ttgatccaaa tgcattgtct
      361 gcgagtttgt tcaacaattt taaccgatta gcacaatctc aagatactaa aaatgaattt
      421 tttatcactt ccaatgtgta taagacgtta gctgaaggaa aagatgagca ggggcaacgc
      481 acagttttat ccaatttgag taatgaaaat accagcttta ttattcctga tgcaaagaaa
      541 gatcctaatg caataggtcg tacactatct ttttctgcag agacacctat ggaggcgcag
      601 gacacccttg gtaagttggt tcaattcatt aatagcaaag catttgctgt tgaattagaa
      661 gattttctag tcgatttcca aaatgtgctt actgcattac aatatgaaaa atcattaatt
      721 caggaagatt tatctgtgaa taagtcggtt cagttggaaa acttaaataa agcctatgaa
      781 actgcacaaa aagcaggtat taaagaatat tcaaaagcat ttaatcaatc agatgcatca
      841 aatattgcag cagctttaag tgatactaaa atctcccttt ctgattcaaa attagcagat
      901 agttcatatt tatttatgtt aggggaaaaa tacctaaagg ctcaaattga tgtggctaat
      961 gagaaaggtg ttgtttatcc accacgttat tatcaaattg attaccaact taaacagcta
     1021 gagccgttat ttgaaaaaac aaagacagtt aaaacacaaa cttttagtta ccaagcttcg
     1081 ccgg
//
LOCUS       DANLXQ010000286         1041 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.293, whole genome shotgun sequence.
ACCESSION   DANLXQ010000286 DANLXQ010000000
VERSION     DANLXQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.293"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..509)
                     /locus_tag="PXD94_002677"
     CDS             complement(<1..509)
                     /locus_tag="PXD94_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287471.1"
                     /translation="MTTQVTPQIMRIIGQIVAATYGDDVPTNVQTIILRYPIRGIGFI
                     SSRRELSINNGEIARLMDKIPGDLEDPKDGMPFDCQGAFWLGYYQYCKLSNDVKNYTS
                     KELSIIGESLYGTQWQSNLARDLRLSDARRVREWVAGERKIPFGVWADLTELVKAKKA
                     NLSSILKKLT"
     gene            complement(579..>1041)
                     /locus_tag="PXD94_002678"
     CDS             complement(579..>1041)
                     /locus_tag="PXD94_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287472.1"
                     /translation="GDRNYQLYIDLATGELVSKLGKGLTTRAFDAAVKAFENAFNAKD
                     TAENTAPVESKETEQENKQETTKLVITKPTLKGSEKQIDSANEIINLFIAKIGDTSIP
                     VTATQEQITQAQAMVDNFFAMTNAGKWIEKFKGLNDNSEPKQFWLSVKYGW"
BASE COUNT          254 a          221 c          189 g          377 t
ORIGIN      
        1 gttaattttt taagaattga tgataagtta gcttttttgg ctttaaccaa ttcggtcaaa
       61 tcagcccaaa ccccgaaagg gattttacgt tcaccagcca cccattcgcg gacacggcga
      121 gcatcagata atctcaaatc tctcgctaga ttagactgcc attgagtgcc atacaatgac
      181 tcgccaatga tggatagctc ttttgatgtg taatttttta catcgttact taatttgcag
      241 tattgatagt atccaagcca aaacgccccc tgacaatcaa aaggcatccc gtctttaggg
      301 tcctcgaggt cgcctggtat tttatccatt aatcttgcta tctctccatt gttaatagat
      361 agctctctgc gactagagat aaaccctatg cctcttatag ggtatctcaa gattattgtc
      421 tgtacgtttg ttggcacatc gtcaccataa gtagcagcga caatttgccc gataatgcgc
      481 ataatttgtg gtgttacttg tgtagtcata ataatctcca gttatctgtt tgttttagcc
      541 gtaagccttt gctcttacgg cttatttgtt atctcaatct accatccata ttttacggat
      601 aaccaaaatt gctttggttc gctattatca tttagacctt taaatttttc gatccacttt
      661 ccagcgtttg tcattgcaaa aaaattatct accattgctt gagcttgagt aatttgttct
      721 tgcgttgctg ttactgggat agaggtatca ccaatcttcg caataaaaag attgatgatt
      781 tcgtttgccg aatcaatttg tttttcagag ccttttaatg ttggttttgt gattacaagc
      841 ttagttgttt cttgtttgtt ctcttgctct gtttctttgc tttctaccgg cgctgtattt
      901 tcagcggtgt ctttagcatt aaaagcgttt tcaaaagctt ttacggcagc atcaaacgct
      961 ctggttgtta atcctttgcc aagtttgctc actaactcgc ctgtggctag gtcaatgtac
     1021 aactggtaat tccgatcacc t
//
LOCUS       DANLXQ010000287         1040 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.294, whole genome shotgun sequence.
ACCESSION   DANLXQ010000287 DANLXQ010000000
VERSION     DANLXQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.294"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..174
                     /locus_tag="PXD94_002679"
     CDS             <1..174
                     /locus_tag="PXD94_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6287473.1"
                     /translation="ISGEIQEKIAEYIRQSNDLRQQAQNLLAQAKNNVEFEIENNSFN
                     INELEVRGGGRSC"
     gene            188..922
                     /locus_tag="PXD94_002680"
     CDS             188..922
                     /locus_tag="PXD94_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6287474.1"
                     /translation="MARSSYFARLAEWTLLEHLLVKQPENISVCSFGQSFGTSGRLDA
                     EYYQPKFASLFAQLANFKTMKLGEIVNLQKSVEPGSEAYQTEGIPFVRVSDLSKFGIS
                     QTDKYLHPKDFGNVVRPKKDSILLTKDGTVGIAYRVPQDLNVITSGAIVHLELKTDEV
                     LPDYLALALNSPAVQLQAERDAGGSIIQHWKPSEILDVVIPVLPKNIQQTISDKVQQS
                     FALRVESEVLLEKAKILVEQEIENMR"
BASE COUNT          363 a          167 c          209 g          301 t
ORIGIN      
        1 ataagtggag aaatccaaga aaaaattgct gaatatatcc gccaaagcaa cgatttaagg
       61 cagcaagcac aaaatttact cgctcaagct aaaaataatg tagagtttga aattgaaaat
      121 aattcattta atatcaatga gttagaagtg cggggggggg gtagatcctg ctaagcctta
      181 tggtttattg gcgagatctt cttattttgc acgtttagca gaatggactt tattagaaca
      241 tttacttgtc aaacagcctg aaaatatttc ggtatgttca ttcggtcaat cattcggtac
      301 aagcggtcgt ttagatgcag aatattacca acctaaattt gcaagcttat ttgcacaatt
      361 agccaatttc aaaacgatga aattaggtga gattgttaat ttacaaaaat cggtagaacc
      421 gggctcggaa gcttatcaaa cagagggaat accctttgtg cgtgtatcgg atttatctaa
      481 attcggcatc agccaaacag ataaatactt acatccgaaa gattttggca atgttgtaag
      541 acctaaaaaa gacagtatat tactgactaa agacggcaca gtcggcattg cttaccgtgt
      601 tccgcaagat ttgaatgtga ttacatcagg ggcaattgtg catctagagc ttaaaactga
      661 cgaagtttta cccgattatt tggcgttagc attgaattct cctgctgtac aattacaagc
      721 ggaaagagat gcgggaggct ctattatcca acattggaaa ccgtctgaaa ttttagatgt
      781 agtgattccg gtattaccta aaaatatcca gcaaaccatt agcgacaaag tacaacaaag
      841 ttttgcattg agagtagaaa gtgaagtttt actggaaaaa gctaaaatac ttgtagaaca
      901 agaaatagaa aatatgaggt aataatgcct aatcataatc aagatttgaa aaaagagtgg
      961 tttaataaag caaggataga ttaccactct ccttttttaa tgttatggct cagttgtaat
     1021 agctggtata acttccatta
//
LOCUS       DANLXQ010000288         1021 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.295, whole genome shotgun sequence.
ACCESSION   DANLXQ010000288 DANLXQ010000000
VERSION     DANLXQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.295"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            28..>1021
                     /locus_tag="PXD94_002681"
     CDS             28..>1021
                     /locus_tag="PXD94_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL6287475.1"
                     /translation="MSNKSIPYQSFCWVIGTTSFRTAKLNLKIEAQLLLLDEFFNEVI
                     KESSWNWNNELQEKYYDFMKDRAFLTGEAKRKDKDAREKTSGLVDIGLITEDRLITDA
                     GRELLKVTSSGDFETNNVFNINRDSFIYLKQLLKTSIDVSGSIVRPFIAVVKCLTELE
                     FLSYDEFTYFVPLIRDDESAKQIISDIKLYREGKILPEEIIYKRLMQMENYKLAQEEF
                     IASDVDENLICLVGMNRKSRNYDKPYYKLYENLKKVFLDGENIYESLLNSAKNINQKP
                     GILWRSLLFKTTNIGVVRKNGKSSIHKQCPFINCKNETELKEVFFKYLHVFKAMA"
BASE COUNT          399 a          116 c          199 g          307 t
ORIGIN      
        1 tatcctgtga attggaggaa taagtaaatg tctaataaaa gtattccata tcaaagtttt
       61 tgttgggtta taggaactac aagttttaga actgcaaaac taaatttaaa aatcgaggca
      121 cagttgttgc ttcttgatga gttttttaat gaggtcataa aggaatcaag ttggaactgg
      181 aacaatgaat tgcaagaaaa atattatgat tttatgaaag atagagcttt cctaacagga
      241 gaagcaaaaa gaaaagacaa agatgccaga gaaaaaacat caggattggt tgatattggt
      301 ttaattacag aagatagatt gattacggat gcaggaagag aactccttaa agtaacaagt
      361 agtggggatt ttgaaaccaa taatgtgttt aatattaata gagatagctt tatctatcta
      421 aaacagcttc ttaaaacgag tatagatgta agtggtagta tcgttagacc gtttattgcg
      481 gttgtaaagt gcctgacgga acttgagttt ttaagttatg acgagtttac ttattttgtt
      541 ccgctaatta gagatgacga aagtgcaaaa caaatcatat cggatattaa attatatcga
      601 gaaggtaaaa ttttaccgga agaaataatt tataagaggc ttatgcaaat ggaaaactac
      661 aaacttgcac aagaagaatt tatagcctct gatgttgatg aaaatctaat atgtttagtg
      721 ggaatgaatc gtaaaagcag aaactatgac aagccgtact ataaattgta tgagaattta
      781 aagaaggtat ttcttgatgg cgaaaacatt tatgaatcgc tattaaattc tgcaaagaat
      841 ataaatcaaa aacctggaat attgtggaga agtttactct ttaaaacaac caatattggt
      901 gttgtaagaa aaaatgggaa gtcttcaatt cacaaacaat gcccatttat aaactgtaaa
      961 aatgaaacag aactaaaaga agtgtttttc aaatatttac atgtttttaa agcaatggcg
     1021 a
//
LOCUS       DANLXQ010000289         1012 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.296, whole genome shotgun sequence.
ACCESSION   DANLXQ010000289 DANLXQ010000000
VERSION     DANLXQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.296"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            11..733
                     /gene="arcA"
                     /locus_tag="PXD94_002682"
     CDS             11..733
                     /gene="arcA"
                     /locus_tag="PXD94_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL6287476.1"
                     /translation="MKIPQILIVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLS
                     TNEINLVLMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEV
                     NKLPRSEFRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHP
                     NTPEIIVTIHGEGYRFCGNIEP"
     gene            753..>1012
                     /locus_tag="PXD94_002683"
     CDS             753..>1012
                     /locus_tag="PXD94_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL6287477.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFSDV"
BASE COUNT          355 a          169 c          208 g          280 t
ORIGIN      
        1 ggtttaaaag atgaagatcc cacaaatctt aattgttgag gatgagttag taacaaggaa
       61 tatgctgaaa agcatttttg aaggagaagg atatcaggta tttcaagcat ctaacggcga
      121 ggaaatgcac tctgttctga gtacaaatga aatcaatctt gtgctaatgg acattaactt
      181 accgggaaaa aatggattaa ttctcgcccg agaaattcga gagaatatga agctaccatt
      241 aatgttttta accggtcgcg acaatgaaat tgataagatc ctcggtttag aaataggtgc
      301 cgatgactat atcactaaac cgtttaatcc tcgagaattg actatccgag cgagaaattt
      361 attgcaacga acgatgatag ataaagaaaa cgacgttgta aaagagcaat ctgaaattga
      421 aagctatcgc tttaacggtt ggatcttaga cattaacagt catagtttaa tctcaccaga
      481 aggtgaggta aataaattac cacgtagtga atttagggct ttattacatt tttgccaaca
      541 tcctggaaaa attcaaacac gtgaagattt attgaaaaaa atgaccgggc gggaactaaa
      601 acctcatgat agaaccgttg atgttactat tcgccgtatt cgtaaacatt ttgaagacca
      661 ccccaatacc ccagaaatta ttgtcaccat tcacggtgaa ggctaccgtt tctgtggtaa
      721 catcgaacct taatcaagat aggagtaatt ttatgaaaaa attaactatt gtcgcattaa
      781 cttgcttatt ttctacccat ctattagccg ctcctgtcgg aaatacattt actggcatcg
      841 gtgtcggtgt agatgtaact acaacaaaat acaaagttga aggagtaaaa ggcaaggatt
      901 ccacaggtcc ggcattagtt gtcgattatg gtattgagca aggcaataac tttgtaggcg
      961 ttgtacaagg aaaagcaaaa gtaggtagta caaaagtatt tagcgatgta aa
//
LOCUS       DANLXQ010000290         1001 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.297, whole genome shotgun sequence.
ACCESSION   DANLXQ010000290 DANLXQ010000000
VERSION     DANLXQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.297"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            157..693
                     /locus_tag="PXD94_002684"
     CDS             157..693
                     /locus_tag="PXD94_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287478.1"
                     /translation="MFSIHSKIEAAYSNPNSSLFQFICFIRKYPKALPFGIMMIFIIP
                     PMIMLIKSYIEVNHILKNIENDKINFNHQQNKYDSIINKAKNQNSQENNLTEINSNIQ
                     ILAEKHQLTIESLQWNLEQGKSIELSIIGNSRSLFDFIHSVKQIPYLKYNAISLIKSR
                     QDRKVEMNTTLVILANKE"
     gene            696..>1001
                     /locus_tag="PXD94_002685"
     CDS             696..>1001
                     /locus_tag="PXD94_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287479.1"
                     /translation="MRNWFLFITLFISFSVKANDPFFGEKQETQASGNIAESNVEFAK
                     NTENITACKVPDNLSAKNIVTDFDKMTLIGLVKINEVMRALFIDEKQQLLALKENDLL
                     "
BASE COUNT          404 a          152 c          129 g          316 t
ORIGIN      
        1 aaataatcgg taatttagat aatcaatctg attttcaaag tctagaatct tatttatatc
       61 agcaagattt atttgaaacc aaaaccgagc aaattaatgt taatcataaa aatgaaatcc
      121 aatttatttt cacactgaaa cataaaggta attaatatgt tttctataca ttctaaaatt
      181 gaagctgcat attccaatcc aaacagttca ttatttcagt ttatttgctt tatccgaaaa
      241 tatcctaaag cattaccatt tggcattatg atgatattca tcattccacc tatgattatg
      301 ttgataaaat cctatattga ggttaatcat attttaaaaa atatagaaaa tgacaagatt
      361 aactttaatc atcaacaaaa caaatatgac tcaattatca ataaagctaa aaatcagaac
      421 agccaagaga ataatttaac cgaaataaac agcaacatac aaatattagc tgaaaaacac
      481 caactaacca ttgaaagttt acaatggaat ttagaacaag gtaaaagtat tgaattgagt
      541 attatcggca atagccgatc tttatttgat tttatccatt cagttaagca aataccttat
      601 ttgaaataca atgcgattag cctcatcaaa tctcggcaag acagaaaagt tgaaatgaat
      661 actaccttag tcatattagc taataaggaa taacaatgcg taattggttt ttatttatta
      721 ctttatttat cagtttttct gtaaaagcaa atgatccttt ctttggagaa aaacaggaaa
      781 ctcaagcctc aggcaacatc gctgaaagca atgtggaatt tgcaaaaaat actgaaaaca
      841 tcaccgcttg taaagtacct gataaccttt ctgcaaaaaa tatagtaact gattttgaca
      901 aaatgacact gataggatta gttaaaatca atgaagtaat gcgtgcatta tttattgatg
      961 aaaaacaaca attacttgct ctaaaagaaa atgacttgct t
//
LOCUS       DANLXQ010000291          981 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.298, whole genome shotgun sequence.
ACCESSION   DANLXQ010000291 DANLXQ010000000
VERSION     DANLXQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.298"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(48..251)
                     /locus_tag="PXD94_002686"
     CDS             complement(48..251)
                     /locus_tag="PXD94_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287480.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
     gene            complement(248..739)
                     /locus_tag="PXD94_002687"
     CDS             complement(248..739)
                     /locus_tag="PXD94_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL6287481.1"
                     /translation="MNEDKFFSVDVSNDIHILNLHETLEQAKQSCLNGATEAYEFADD
                     MDDHESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERESELFGEAEQVIEPPTLVENNG
                     WISVKDRLPEIRKEVLITNNFIMIAQFNGESWFKPGGFLGIQPVYGVTHWQPLPEPPK
                     EEK"
BASE COUNT          263 a          203 c          180 g          335 t
ORIGIN      
        1 ttgagccgtt aattaaattg cggtcttccg cccattgttc gatgttttta attagttgtt
       61 gcattgttta gtcctctcat ttctttcatt gcttcatcaa tcgccgtgcg taaatcggca
      121 tttcttgccc aatgaaaata ctgctcgtgc ggtttggtat agctagcaat aataaattcg
      181 tccaccaaat cagaatatcg gacatctaat ttgcgttggg cgataaagtc taagcgttcg
      241 gtgtcggtca tttttcctcc tttggtggtt cgggaagtgg ttgccagtgg gtaacaccat
      301 agacaggctg aatacctaag aatccacccg gcttgaacca gctctcgcca ttgaattgag
      361 caatcataat aaaattgttt gttatcaaga cttctttgcg gatttctggc aatctgtctt
      421 tgactgaaat ccagccgttg ttttctacaa gtgttggtgg ttcaataact tgttcagctt
      481 ccccaaataa ctctgattct ctttcttcat cggttagtgg gcggatatct gattttgctc
      541 tacctaaaac aacaccataa actgcatagg gtaaatcata ggcttcatag ctttcgtgat
      601 catccatgtc atccgcaaac tcataggctt cagtagcacc atttaaacag ctttgttttg
      661 cttgctctaa ggtttcgtgc agatttagga tatgtatatc atttgataca tccaccgaaa
      721 aaaatttgtc ttcattcatt ttctttctcc aaaataccaa tagttgagag aataataagc
      781 atactatctc atgctattct taagtgcatc taaccacgct ccagcatctt cggctgaagc
      841 gaaatataac ccttgatcaa atagtttttc tatataagaa tgtgtaggga aatttgattt
      901 tatcacattg aaattatcgc caataaacca cattccatct cttggttctt ttaatggaca
      961 aggaagagtg agagttactg t
//
LOCUS       DANLXQ010000292          979 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.299, whole genome shotgun sequence.
ACCESSION   DANLXQ010000292 DANLXQ010000000
VERSION     DANLXQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.299"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            134..820
                     /locus_tag="PXD94_002688"
     CDS             134..820
                     /locus_tag="PXD94_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287482.1"
                     /translation="MAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTITGT
                     TISGGTISGTTITGGIIKGARIEGLTGKFTGELEINQLIGGNIYEAKTWTSKATGKSK
                     TYSETKNSFALSDTYYEYVCQFVLPATKATRTLYVVHTPTGESKSETFYGSGSNRTPT
                     TITLPSVIDISNTISPNVSNPFNDSGAILIANTQYVVTCRAWCAGRGRSAKVNFRAII
                     SSTNGSSYQL"
BASE COUNT          344 a          178 c          202 g          255 t
ORIGIN      
        1 cccttggcaa aatgccggtg taaccgctat ccacggtggc tcaatagtta ctaataccgt
       61 tactgctcaa caaattgcag caaataccat taccgccaac gaaatcgcag ccggcacgat
      121 tgcagctcga aatatggcag caaactcgat taatgcaagt catattgtgg ggagttcgat
      181 cactgcggat aagctaaatg taaacaattt agcggcaata tcggcaaatc ttggtaaggt
      241 aactgctggt acaattacag ggacgacaat tagtggtggg acaatttcag gtacaacaat
      301 tacaggggga ataattaaag gagcaagaat tgagggatta acaggtaaat ttactggaga
      361 acttgaaatc aaccaattga tcggaggtaa tatctatgag gctaaaactt ggacatcaaa
      421 agcaacaggg aaaagtaaaa catattcaga aacgaaaaac agttttgcat taagtgatac
      481 atattatgag tatgtatgtc aatttgtact acctgctaca aaagcgacga gaacattgta
      541 tgttgtccac actccaacag gagagagtaa aagcgaaact ttttatggta gtggtagtaa
      601 tcggacaccg acaacaatta cattaccgtc tgtaattgat attagcaata ctatttcacc
      661 taatgtatct aacccattta atgattctgg ggcaatactc attgctaata ctcaatatgt
      721 agtaacgtgt cgtgcgtggt gtgcagggcg tgggagaagt gctaaagtga attttcgagc
      781 aattatatca tctacaaatg gttcatctta tcaattatag gaggcaaata tgaaaaaaat
      841 tatactactt acaacaatta tggtttcagc ttgtacaact caatataagc aagactatac
      901 aacaaaacca accttttgtt atcaactagc ccctcaagag caattaccgg ggaaaaactg
      961 tattggctca ggagggcat
//
LOCUS       DANLXQ010000293          973 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.300, whole genome shotgun sequence.
ACCESSION   DANLXQ010000293 DANLXQ010000000
VERSION     DANLXQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.300"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            131..379
                     /locus_tag="PXD94_002689"
     CDS             131..379
                     /locus_tag="PXD94_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287483.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
     gene            391..594
                     /locus_tag="PXD94_002690"
     CDS             391..594
                     /locus_tag="PXD94_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287484.1"
                     /translation="MKIQFPISYQEFRENYFEKKPLLMKGAISQKDLLSWKSINEILP
                     RCDLISEDAIKVMHKGKRAHKKD"
     gene            633..>973
                     /locus_tag="PXD94_002691"
     CDS             633..>973
                     /locus_tag="PXD94_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046701250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL6287485.1"
                     /translation="MKITHCKLKKSLQKKLLEFFVAEVTARTAADLLGIQPNTAALFY
                     HKIRLVIEHHLALEAHEIFEGKIEVDESYFGGHRKGKRGRGAAGKVAVFGLLKRQGKV
                     FTKYQIRNANAG"
BASE COUNT          314 a          134 c          239 g          286 t
ORIGIN      
        1 attataaaca tcatagagga atatctctta tgaaagagtt aaaaattgag gattgccaat
       61 ttgtttcggc aggcttgcac ttgttattgt aggagctgtt gctggagctg catattatgt
      121 tgggaatggt atgatttctg gggaaaaaat gacttgggag agtttgggag catcagctat
      181 aggtggggct attggtggcg gtattggagc aacaatgaaa gctggtgcgg tgacagcgtc
      241 aaccttatct gctttaggtg gtggtatggg ggagggcttg tataaaagta cttctcaagg
      301 tatcaaaaat agtattggta taaaggcgag ttgcaatcaa tcaggttcgg attatggtga
      361 tggatgtgat tatcaataag agagtatatt atgaaaattc aatttccaat ttcttatcaa
      421 gaatttcgtg aaaactattt tgagaaaaaa ccacttttaa tgaaaggagc aatatctcag
      481 aaagatttac tttcttggaa gtcaataaat gaaatattac ctcgctgtga tttaatttct
      541 gaggatgcaa ttaaggtaat gcataaaggg aaaagggctc ataaaaaaga ttaggggctg
      601 tagtagatta gcatcactgc tatactacaa aaatgaagat aacccattgt aaattaaaga
      661 aatctttgca gaaaaagctt cttgaatttt ttgttgctga agtgaccgct agaaccgctg
      721 ctgatttatt aggtattcaa cccaatacgg cagccctgtt ttaccacaaa attaggctgg
      781 tcattgaaca ccatttagcc cttgaagctc atgaaatttt tgagggaaaa attgaggtag
      841 atgaaagcta ttttggtggt catcgcaaag gaaaacgagg gcgaggtgcg gctgggaaag
      901 tcgctgtatt cggtttgttg aagcgtcaag gaaaggtgtt tacaaaatac caaatcagaa
      961 acgctaatgc cgg
//
LOCUS       DANLXQ010000294          964 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.301, whole genome shotgun sequence.
ACCESSION   DANLXQ010000294 DANLXQ010000000
VERSION     DANLXQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.301"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            223..964
                     /locus_tag="PXD94_002692"
                     /pseudo
     CDS             223..964
                     /locus_tag="PXD94_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
BASE COUNT          343 a          167 c          151 g          303 t
ORIGIN      
        1 gactcctcta atagtccact cttgttccat tttggaacat ttttgtatat tatttcgcct
       61 tttctttctc attgtgttct atgtaggcaa cagcattttt tgcaaagtga tcgacgagaa
      121 cattcattac ttcagtccat tttacaggtc ttcctatctt cattccaact tccatcgcta
      181 atctttctat cttgagtttt ttctctaatt taatattgaa cagagcttta tgaaatggtt
      241 gatatagaac aaacgtcagt tgattggatt gatgtaactt attcagctca tattgattcg
      301 gaatctatcc aaaaacaact tatctctttt ttccaaaaca tccatttagg acaaacacgt
      361 cgtacaagac ataacaaaga atatgacaca acggcagaat ggaattcagg ttcagaacac
      421 cgtattttaa aagtttatct aaaaggtaat gaagttcaaa aaagactaca agaagcccag
      481 cacgacttca aaaaatcacc aagtaaaaag tatctgcaaa actcaattca agtattaagt
      541 aatccaaatc tcattgaatt ttcaaaaaaa tgtgttcgtt ttgaagcaag attaaaacaa
      601 cgttatttag ataaacatca aatcccacgc aatttatttg acttaattaa atatcagaga
      661 gattacgaag agcagggtag aagcctgatt aaagacctat ggcaagacgc attcaaagac
      721 attattaagg ctgtcgggga aagcaatatg aatatttaca acagagatca aattcagcgt
      781 ttattacgga aaaattattt ttcaatcact ccaaaaggaa atatgagcta tgcaaaggct
      841 gatcgtttat tcagctttta taagaattta ctaagtgatg gctatagaga aacgctagat
      901 tctatgccaa gaaacacatt ttggagatat gaaaaggatc ttatggccgt tggtttaact
      961 aaaa
//
LOCUS       DANLXQ010000295          962 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.302, whole genome shotgun sequence.
ACCESSION   DANLXQ010000295 DANLXQ010000000
VERSION     DANLXQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.302"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(25..>962)
                     /locus_tag="PXD94_002693"
     CDS             complement(25..>962)
                     /locus_tag="PXD94_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287486.1"
                     /translation="LRSLKHIKNLESNSYLDEEGRDTVNKYKAFFSIFEAEIRDATCI
                     CDNKIVTINGEVCCDIKEVARKIVIEFLNEVDIGNKVKFYYPDERHELFVELLIVLAT
                     LRIYRNRNIFFFEKGFFNFFPEFITILGTGAFNGFLDFPSLVGKVFPEIIDTINEGLD
                     LDIAIENFEVLSQEEMTECMGLYEQALIKKAEEGEIGLLNVDEINSFYEDNSELILLA
                     KQPMVEEIIPKPDSKASSAILKFFDSNSNILIAKLKESGLQLSAVALQNDQNIARVAN
                     VVYNLLPGMVRIFVSYDTVENFLLNNRQWLINKLV"
BASE COUNT          316 a          193 c          115 g          338 t
ORIGIN      
        1 tatccatagt tcttcccctc caaattaaac taatttatta atcaaccatt ggcggttatt
       61 taataagaaa ttttctacgg tatcgtaact tacgaaaatt cttaccatgc ccggcaataa
      121 gttataaacg acatttgcta ctcgtgcgat attttggtcg ttttgtaatg ccacagcact
      181 taattgcaaa ccactttctt ttaatttagc aattaagata tttgagtttg aatcaaagaa
      241 ttttagaatt gcagaggagg ctttgctatc tggtttaggg attatctctt caaccatagg
      301 ttgtttagca agtaagatta gctcactatt atcttcataa aagctattta tttcatcaac
      361 atttaataaa ccaatttcgc cttcttcagc tttcttaatg agagcctgct catataaacc
      421 catacattca gtcatttctt cctgactcaa cacttcaaag ttctcaatcg caatatctaa
      481 atctaaaccc tcatttatcg tatcaataat ttctggaaaa acctttccta ccaaactagg
      541 gaagtctaaa aatccattaa acgcaccagt cccgagaata gtgataaatt cgggaaaaaa
      601 gttaaaaaac cccttttcaa agaagaatat atttctatta cgatatattc ttaatgttgc
      661 tagaacaatg agcagctcta caaaaagctc atgtctttca tctgggtaat aaaacttaac
      721 tttattacca atatccactt catttagaaa ttctataaca attttccttg ctacctcctt
      781 aatatcacag cacacctcac cattaattgt tacaatttta ttgtcacaaa tacatgttgc
      841 atctcgaatc tctgcttcaa aaatagagaa aaatgccttg tatttattta ccgtatctct
      901 tccttcttca tctaaataac tgttagattc tagatttttg atatgtttca aagatcttaa
      961 gt
//
LOCUS       DANLXQ010000296          946 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.303, whole genome shotgun sequence.
ACCESSION   DANLXQ010000296 DANLXQ010000000
VERSION     DANLXQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.303"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            99..>946
                     /gene="msrA"
                     /locus_tag="PXD94_002694"
     CDS             99..>946
                     /gene="msrA"
                     /locus_tag="PXD94_002694"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL6287487.1"
                     /translation="MKYNQLLLCLVSAIFTTSSIANITTPNEMRSENLQDIYLAGGCF
                     WGIEAYMEKIYGVKEATSGYANGKTEQTAYRIIGQTDHAETVHVTYDANKISLEKLLQ
                     YYFKVIDPTSKNKQGNDIGRQYRTGIYYQNEQDKQIILKEIQAQQAKYKQKIQVQVEP
                     LKHYILAEEYHQDYLKKNPNGYCHIDLNQANSIIIDPKDYPKPSDATLKMKLTPLQYS
                     VTQRKNTERSFSNEYWDNTAPGIYVDITTGEPLFSSLDKYDSGCGWPSFTKPIINEVV
                     NYTEDN"
BASE COUNT          363 a          169 c          160 g          254 t
ORIGIN      
        1 aattttgatc ttagcaaaaa agtaagaaat tatgaatgtt taaggttaaa ccctcatcct
       61 taaaaaataa aattacataa gttataaagg agaactggat gaaatacaat caactgttac
      121 tatgtttggt gtctgctatc tttaccacct cctcaatagc aaacatcaca acccctaacg
      181 agatgcgatc tgaaaactta caggatattt acttggcagg aggatgtttc tggggcattg
      241 aagcctatat ggaaaaaata tatggcgtaa aagaggctac atcgggctat gccaatggca
      301 aaactgagca aactgcttac cgaattatcg ggcaaaccga tcatgcagaa acagttcacg
      361 tgacctatga tgctaataaa atttctttag aaaagctgtt gcaatattac tttaaagtaa
      421 ttgatccaac cagtaaaaat aaacaaggta atgatattgg ccgccaatat cggacaggta
      481 tttattatca gaatgagcaa gataaacaaa ttattttaaa agaaatacaa gctcaacaag
      541 caaaatacaa acaaaaaata caagtgcaag tagaaccatt aaaacattat attttggcag
      601 aagagtatca tcaggattac ctaaagaaaa atccaaatgg ctactgccat attgatttaa
      661 accaagccaa cagcattatt atcgatccga aagattatcc taaacctagt gatgcaacat
      721 taaaaatgaa attaacgcct ttgcaatata gcgttaccca acgtaaaaat actgagcgtt
      781 cattcagcaa tgaatattgg gataataccg caccgggcat ttatgttgat ataactacag
      841 gagagcctct tttttcatct ctcgataaat atgactccgg ctgtggctgg ccaagtttta
      901 ccaaaccaat tatcaatgag gttgttaact ataccgaaga taatag
//
LOCUS       DANLXQ010000297          891 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.305, whole genome shotgun sequence.
ACCESSION   DANLXQ010000297 DANLXQ010000000
VERSION     DANLXQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.305"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(6..491)
                     /locus_tag="PXD94_002695"
     CDS             complement(6..491)
                     /locus_tag="PXD94_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287488.1"
                     /translation="MIQNDPRDAIILSLLANAVYGGHNNSQISYQETEEVNLYARLRQ
                     VEGDVREIKSNYLTKEAFYKAGGSTLIALVVTAGIALWTVYSHLDTKVEARFSKIDDK
                     FVQVETNIKNLDIRLTKVESRLDNVEQRLDNVEQRLGNMEKKIDGVDNKLDLLLQQFK
                     R"
     gene            complement(496..876)
                     /locus_tag="PXD94_002696"
     CDS             complement(496..876)
                     /locus_tag="PXD94_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287489.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
BASE COUNT          251 a          178 c          143 g          319 t
ORIGIN      
        1 ttattttatc ttttgaattg ttgtagtaac agatcgagtt tattatcaac cccatctatt
       61 ttcttctcca tattgcctag tcgctgctca acattgtcta gtcgttgttc gacattatcc
      121 aatctagatt caactttagt gaggcgaata tcaagatttt tgatattggt ctctacttgg
      181 acaaacttat catcaatttt cgagaatcga gcttcaactt tggtgtcaag gtgtgaatat
      241 actgtccata atgcaattcc agcagtaact actaatgcaa tgagtgtaga gccaccagct
      301 ttataaaaag cctctttcgt caaataattt gacttgattt ctcttacatc gccttcaact
      361 tgtcttaatc gagcatataa attcacttct tccgtctcct gataactaat ttgtgaatta
      421 ttatgcccgc cgtatactgc atttgcaagt aaggatagta tgattgcatc tctaggatca
      481 ttttgaatca ttggtctatt cattttgtga gcccttaatc cattctaaaa ttctctctct
      541 gtcgaaaaaa agcatattat tacagcaaac acaagtgaca ataacggctg ttctcgcttt
      601 ttggtctaac caatattttg ttccttcccg ttcattgaaa tgcaaagaat gaaattcagg
      661 agctgcaaat ttcataactt catcaggaga tggctctatt cggtatggaa tggttggaac
      721 accgataatt ttctctccgc ataattccgt aaaagtgtca tagccatcgt gtaaataatg
      781 ttcttggcta ttacaaatag ggcaagttaa attccgccca gtacgttgcg tgataaaatc
      841 cgcaaactgt tcaggtttaa tgcgtttatt attcatttta acttcctttc t
//
LOCUS       DANLXQ010000298          887 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.306, whole genome shotgun sequence.
ACCESSION   DANLXQ010000298 DANLXQ010000000
VERSION     DANLXQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.306"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>887)
                     /locus_tag="PXD94_002697"
     CDS             complement(<1..>887)
                     /locus_tag="PXD94_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6287490.1"
                     /translation="SQLKEGDVLVNIVGATLEVVGRVAYVSSTFPKANITQAMSFVRL
                     KSKYNKELLPTYLFAFLQSSYGKIQINRNARPTGQYNLNNEELGAIKVPLIDLETQKQ
                     VDKIIKQSNDFVQKSTQSYQEAETLLLENLGLRAFQADSNPVNVKSLKESFLQTGRLD
                     AEYYQTKYEQYWNLIQSQDYVFIRDEYLHITQKPDWTKPMYQYIEIGDVNIGDGSYQT
                     NWIETQELPANAKTQAQTGDILISTVRPYRGAVTIIGENDQDLVVSGAFTVLRRKENS
                     VFNNEVLKVLLRSELYKDW"
BASE COUNT          272 a          161 c          128 g          326 t
ORIGIN      
        1 accaatcttt atatagttct gaccgtaata gcacctttaa aacttcatta ttaaaaacag
       61 agttttcctt tctgcgtaat actgtaaacg caccactaac aactaaatct tgatcatttt
      121 caccgataat tgttactgca ccacgataag ggcgaacggt tgaaattaaa atatcacctg
      181 tttgggcttg agttttagca ttagcaggta attcttgtgt ctctatccaa ttcgtttgat
      241 atgaaccatc accaatatta acatcaccaa tttcaatata ttgatacatc ggttttgtcc
      301 aatcaggttt ttgggtgata tgtaaatatt catcacgaat aaaaacataa tcttgcgatt
      361 gaattaaatt ccagtattgc tcatatttag tttgataata ctctgcatct aatcgtcctg
      421 tttgtaagaa actctctttt aaggatttaa catttacagg attgctatca gcttgaaaag
      481 ctctcaaacc gagattttct aataataaag tttcagcttc ttggtaactt tgtgttgatt
      541 tttgtacaaa atcattactt tgcttaatga ttttatctac ctgcttttgt gtttctagat
      601 caattaaggg gactttaatc gcaccaagct cctcattatt cagattgtat tgtcctgttg
      661 ggcgtgcatt ccggttaatt tggatttttc cataagaact ttgtaaaaaa gcaaataaat
      721 acgttggtaa taattcttta ttatattttg attttaatct tacaaaagac attgcttgtg
      781 taatatttgc ttttggaaaa gtgctactaa cataagctac ccgaccaaca acctccaaag
      841 ttgcaccaac gatatttact aaaacatccc cttctttcag ttgtgat
//
LOCUS       DANLXQ010000299          863 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.307, whole genome shotgun sequence.
ACCESSION   DANLXQ010000299 DANLXQ010000000
VERSION     DANLXQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.307"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            36..746
                     /locus_tag="PXD94_002698"
     CDS             36..746
                     /locus_tag="PXD94_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287491.1"
                     /translation="MWKKIRIDFTDTVKCQFCSRMITSGKAIVVQNELGNISFSGPSC
                     AQNKNGQNVVNPKEKIVDITKGCLLHEPTTNDKTDKQVDVEQDNFTSIHDDEVHNYLD
                     NNAVNAYLTLRFEKLSHLTSIIKSQKLNEIYHNYELTGQISQKDESYIRVIMYGEAYP
                     LYTYQNLQAVYAAEYWLQSFIQHNPENDLNFIQDTLNQLRRKLTLTEKQIMGINNWFK
                     NSDGKMVKLKTNAFVRGK"
BASE COUNT          321 a          121 c          150 g          271 t
ORIGIN      
        1 gtatttttat tactcactat atggagagat atgttatgtg gaaaaaaatt aggattgatt
       61 ttactgatac agtgaaatgt caattttgca gtagaatgat tacatcaggc aaagctattg
      121 ttgttcaaaa tgaacttggc aatatctctt tttcaggacc gtcttgtgct caaaataaga
      181 acggtcagaa tgtagtcaat cctaaagaga aaatagttga tatcacaaag ggatgtttac
      241 ttcatgagcc aacaacaaat gataaaacgg ataagcaagt agatgttgag caagataatt
      301 tcacatccat acacgatgac gaagtacata attatttgga caataatgct gttaatgcat
      361 acctgacctt aagatttgaa aaactatcgc atttaacctc gattataaaa tctcaaaagt
      421 taaatgaaat ctatcataac tatgaactta cagggcaaat atctcaaaaa gatgaaagtt
      481 acataagagt gattatgtat ggtgaggcat atcctttgta tacataccaa aacttacaag
      541 ctgtttatgc tgctgaatat tggcttcaat catttattca gcataatcca gagaatgact
      601 taaattttat acaagacaca ttaaatcagc tcagaagaaa gctaacatta actgaaaaac
      661 agattatggg cataaataat tggtttaaga attctgatgg aaaaatggtg aaattaaaga
      721 caaatgcttt tgtaagaggg aaatagttaa cataattgtg gtggccactt attttgttac
      781 gtaggcccga cgtaagactg gttttaaaag actctttcct attatttgct tgcttatttt
      841 gacaaattca agttatatgt aat
//
LOCUS       DANLXQ010000300          851 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.308, whole genome shotgun sequence.
ACCESSION   DANLXQ010000300 DANLXQ010000000
VERSION     DANLXQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.308"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            29..802
                     /locus_tag="PXD94_002699"
     CDS             29..802
                     /locus_tag="PXD94_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL6287492.1"
                     /translation="MNLSNALKLIKYNELDVLEVNHNKFKAKIALQGAQLLSWQPYNA
                     EQDVLWLSEIEPFRIGSAIRGGIPICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENV
                     GLVFALENKAKMEMMLGETCELYFTHLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCF
                     DKLTDQEVEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETVLWNPWH
                     NTVSAMTETAYQNMVCVETARINTLLQQNETLGVRVLVR"
BASE COUNT          281 a          134 c          188 g          248 t
ORIGIN      
        1 attattttag tcatcaaagg aataaattat gaacttgagt aatgcattaa aattaatcaa
       61 atataatgaa ttagacgtgc tagaagttaa tcacaataaa ttcaaagcta aaattgcatt
      121 acaaggagca caattgctaa gttggcagcc gtataatgca gagcaagatg tgctgtggtt
      181 aagtgagata gaaccgttta gaattggctc tgcgattcgt ggcggtatac caatttgtta
      241 tccgtggttc ggttcaataa aagagcctgc ccacggcaca gctcgcattc aagaatggcg
      301 tttagttgag catacttgtt ctgatgaaaa tgtcggtttg gtatttgcct tagagaataa
      361 agcgaaaatg gaaatgatgt taggcgaaac ttgtgagtta tattttaccc atttagatcc
      421 agaaccagca caactggcat tgcactctta ttttaatatt ggtgatattg aaaaagtgga
      481 agtgcaaggc ttgccaactc gctgttttga taagctaacc gatcaagaag ttgaagtgcc
      541 atcacctcgc aagataagtg aaaatgtgga ttgtatttat ccggcacaag cggtgaattt
      601 tattcaagat tttgcaaatc agcgcaccat taaagttgag catattaatg cgaccgaaac
      661 cgtactttgg aatccttggc ataataccgt tagtgcgatg acagaaacgg cgtatcaaaa
      721 tatggtatgt gtggaaaccg ctcggataaa tactctactt caacaaaatg aaacgctagg
      781 agtgagggtt ttagtgcgat agtgtttaat gagagcaagc agctgaatta gtaatattgt
      841 ttacaaattt a
//
LOCUS       DANLXQ010000301          842 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.309, whole genome shotgun sequence.
ACCESSION   DANLXQ010000301 DANLXQ010000000
VERSION     DANLXQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.309"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            64..687
                     /gene="tetR(H)"
                     /locus_tag="PXD94_002700"
     CDS             64..687
                     /gene="tetR(H)"
                     /locus_tag="PXD94_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(H)"
                     /protein_id="HDL6287493.1"
                     /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWH
                     VKNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAG
                     TRPSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREK
                     VETDTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
     gene            complement(690..827)
                     /locus_tag="PXD94_002701"
     CDS             complement(690..827)
                     /locus_tag="PXD94_002701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287494.1"
                     /translation="MPENGVIFSDGIEDDYLQFNAGCIAHIGIADIQGQLISQKGRQR
                     I"
BASE COUNT          264 a          151 c          179 g          248 t
ORIGIN      
        1 agtgggtgta atatatctct atcagtgata gattgtcaag gctattttat tttgaggtag
       61 gtaatggcaa agctagataa agaacaagtt attgataatg cgttgatttt acttaatgaa
      121 gttggtattg aaggattaac aacgcgtaag ctggcgcaaa aaataggtgt ggaacaaccc
      181 acattgtatt ggcatgtaaa aaataaacgc gctttgttag atgcattagc agaaactatt
      241 ttgcaaaagc accatcatca tgttttgcca ttgccgaatg aaacatggca ggactttttg
      301 cgaaataacg cgaaaagctt ccgccaagcc ttattaatgt atcgtgatgg tggcaaaatt
      361 catgcgggaa cacgcccctc tgaaagtcaa tttgagacat cagaacagca actacagttt
      421 ttgtgtgatg ctgggtttag tctatctcaa gccgtgtatg cattaagctc tattgcgcat
      481 tttacattag gctccgtact ggaaactcaa gagcatcaag aaagccaaaa agagcgtgaa
      541 aaagtagaga cggatactgt tgcctatccg ccattattaa cccaagccgt tgcaattatg
      601 gatagtgata atggtgatgc tgcatttttg tttgtccttg atgtgatgat ctctggactt
      661 gaaacagtat taaagagcgc taaataaatc taaattcgct gacgcccttt ttggctaatt
      721 agttgccctt gtatgtcagc gataccaatg tgagcaatac aacctgcatt aaattgtaaa
      781 tagtcatctt cgatgccatc agagaaaatt acgccattct ctggcatcaa agatcctaat
      841 tg
//
LOCUS       DANLXQ010000302          817 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.310, whole genome shotgun sequence.
ACCESSION   DANLXQ010000302 DANLXQ010000000
VERSION     DANLXQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.310"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..208)
                     /locus_tag="PXD94_002702"
     CDS             complement(<1..208)
                     /locus_tag="PXD94_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287495.1"
                     /translation="MTKTKHKHPLAEAFYQLGKDVGEINRRLVVVDEQSLKLQELKQT
                     INEQTEQIKTLGTVVNKLIDALQQS"
     gene            complement(201..350)
                     /locus_tag="PXD94_002703"
     CDS             complement(201..350)
                     /locus_tag="PXD94_002703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287496.1"
                     /translation="MDNILITIHPTETIDITIDKLVVIHIGEEYGDTPDFLTIYQQAK
                     ELNDD"
     gene            complement(584..>817)
                     /locus_tag="PXD94_002704"
     CDS             complement(584..>817)
                     /locus_tag="PXD94_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287497.1"
                     /translation="QDDGSLNAKYVVGTGEESDSEVTNFVIITSEYKYIDAQSAKAIT
                     DAPLTKEDIGKTPTQIMLGRIYNHLKETGQIVV"
BASE COUNT          193 a          167 c          161 g          296 t
ORIGIN      
        1 gtgactgttg taatgcatca ataagtttat ttaccactgt tccgagtgtt ttaatttgct
       61 cggtttgctc attaatagtc tgctttaact cttgcagttt taatgattgc tcatccacaa
      121 ccactaatcg acggttaatc tctcctacgt ctttacctaa ttgataaaat gcttctgcaa
      181 gtgggtgttt gtgtttagtt ttagtcatcg ttaagctcct ttgcttgttg gtatatagtt
      241 aaaaagtcag gtgtgtcacc atactcctca cctatatgga taactaccag tttgtctatg
      301 gtgatatcaa tagtttcggt tggatgtata gtgattaaga tattgtccat attcccgcct
      361 taaatttggg tttggtcagg gataagttgg atcgtgccgt agtactgcca ttttgggtgg
      421 atagctcaat ttgaggctct gtgctgccct ctactatggc gtgtaaatcc agcctaatac
      481 cctctaaatt tacaggctcg ccatttgttt gcctaaaaaa aaaggcgata acttacgtca
      541 tcgcctctga aaaacttaaa tttatgcttt tgcatttaga cccctacact acaatctgac
      601 cggtttcttt gaggtggttg tagatacgtc ctaacataat ctgcgttggc gttttgccaa
      661 tatcctcttt tgttagcggt gcatcagtaa tcgctttggc tgattgagca tcaatatatt
      721 tgtactcact tgtgatgatt acaaagtttg ttacctcgct atcgctctcc tcacctgtgc
      781 caacgacata tttagcattg agtgagccgt cgtcttg
//
LOCUS       DANLXQ010000303          814 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.311, whole genome shotgun sequence.
ACCESSION   DANLXQ010000303 DANLXQ010000000
VERSION     DANLXQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.311"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..513
                     /locus_tag="PXD94_002705"
     CDS             <1..513
                     /locus_tag="PXD94_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDL6287498.1"
                     /translation="TYIKEGEDAEYHKRYKCKIRKPWYKVPSVYSTNIGMLKRSHEAP
                     KLILNKLGAYTTDTAYRIKSNKFQDEILVCCFLNPLTMILSELNGRFYGGGVLELVPS
                     EIRNLYIPIIENIDFNIEELNEEVKAGNIENVIRNNGEKILTNLGFSQEDNAMLMSIW
                     KKLKNNRLRR"
     gene            complement(558..>814)
                     /locus_tag="PXD94_002706"
     CDS             complement(558..>814)
                     /locus_tag="PXD94_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287499.1"
                     /translation="QKQIPIPNINFNNPAERLTHDEISDLQKELNQLFSKIEISNERY
                     GVMYRRMFDDKKKKLDDKIKELYNLNELDDIIPSIEDLYS"
BASE COUNT          291 a           95 c          140 g          288 t
ORIGIN      
        1 acttatataa aagaaggtga ggatgcggag tatcacaaaa gatataaatg caaaataaga
       61 aaaccatggt ataaagttcc atcggtttat tcaactaaca taggaatgct gaaaagaagt
      121 catgaagcac caaaactaat attgaataaa ttgggggctt atactaccga tactgcgtat
      181 cgtataaagt caaataaatt tcaagatgag attcttgtgt gctgtttcct aaatccatta
      241 actatgattt tatctgagtt aaatggaagg ttttatggtg gaggcgtatt agaattagtt
      301 ccttctgaaa ttagaaatct atatattcca ataattgaga atattgattt taatatagaa
      361 gaattaaatg aagaagttaa agctggaaat atagagaatg tcataagaaa caatggtgaa
      421 aaaattttaa caaatttagg cttttctcaa gaagataatg ccatgttaat gagtatatgg
      481 aaaaagctaa aaaataacag gttaagaaga tagcttgaaa aggttgtgtg gtttatacat
      541 cacacaacct tttagaatta actatataga tcctctatag atggtataat gtcatctaat
      601 tcgtttaggt tgtatagttc ttttatctta tcatctaatt ttttcttctt atcatcaaac
      661 attcttctat acataacccc atatctttca ttagagattt ctatttttga gaaaagttga
      721 tttaactctt tttgtaaatc tgatatttca tcgtgagtta atctttctgc tgggttattg
      781 aagtttatgt tagggattgg tatttgtttt tgga
//
LOCUS       DANLXQ010000304          809 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.312, whole genome shotgun sequence.
ACCESSION   DANLXQ010000304 DANLXQ010000000
VERSION     DANLXQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.312"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>809
                     /locus_tag="PXD94_002707"
     CDS             <1..>809
                     /locus_tag="PXD94_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6287500.1"
                     /translation="FMEKVEEFTETLKTLSRDDLSAEERTTKTIDSVKLRIYLNEQAR
                     NRKNYINGFVPTDWIPEITDQMTKSKNLWLKYEEEAKTTLKPYQEETISRVIQDEINK
                     KMAEIGTTTLEIGGKEHELEDLENQINALALTDEEKLAEDVENDLISKLDEAKAALAK
                     NQQTLKEKRDELLGLNNKLDSSPLVKKGKNALAEGTNAFASGENAIAFGTDSQATGNN
                     AIALGANSKANAESAIAIGKGAQALKEKALALGENAIANRASAIAIGDNHS"
BASE COUNT          328 a          135 c          156 g          190 t
ORIGIN      
        1 ttttatggag aaggtagagg aatttactga aacattaaaa actttatcta gagatgactt
       61 aagtgcagag gaacgtacaa ccaaaacaat tgactccgtt aaattaagaa tatatctcaa
      121 tgaacaagca cgaaatcgta aaaattatat taatggattt gtacctaccg attggattcc
      181 tgaaattaca gatcaaatga caaaatccaa aaatctatgg ttaaaatatg aagaggaagc
      241 caaaacaaca ttaaaaccat accaagaaga aacaatatct agggttatac aagatgaaat
      301 taataaaaaa atggctgaaa taggtactac aacactagag attggtggta aagaacacga
      361 gctcgaggat cttgaaaacc aaattaatgc tttagcttta actgatgaag aaaaacttgc
      421 ggaagatgtg gaaaatgatt taatctcaaa gcttgatgag gctaaagcag ccttagcaaa
      481 aaaccagcaa acgctcaaag aaaagcgtga tgaattacta gggctaaata acaaattaga
      541 tagctcacca cttgttaaaa aaggtaaaaa tgcacttgca gaaggcacga atgcctttgc
      601 ttctggtgaa aatgccatcg cattcggcac ggattcacaa gctacaggta ataacgctat
      661 cgcactaggt gcaaattcaa aagcaaacgc agaaagtgcg attgcaattg gtaaaggtgc
      721 acaagcatta aaagaaaaag cccttgcttt aggcgaaaat gccattgcca acagagcatc
      781 tgctattgcg attggtgata accattcat
//
LOCUS       DANLXQ010000305          802 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.313, whole genome shotgun sequence.
ACCESSION   DANLXQ010000305 DANLXQ010000000
VERSION     DANLXQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.313"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            130..519
                     /locus_tag="PXD94_002708"
     CDS             130..519
                     /locus_tag="PXD94_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL6287501.1"
                     /translation="MADPIEWSIITTDCFDEWFGQQDESTQERVLACLIYLRKFGYSL
                     GRPYADTVYQSKFPNMKELRIQNKGKPIRAFYAFDPLRQAIVLCAGEKGKDKRFYEKM
                     IALADAEFTSYLNNLEKENENVRSNAQ"
     gene            491..790
                     /locus_tag="PXD94_002709"
     CDS             491..790
                     /locus_tag="PXD94_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL6287502.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
BASE COUNT          280 a          138 c          152 g          232 t
ORIGIN      
        1 tggattgttg ataaaaaaag accgcttgta gtcatacaaa cggcttagta ggaagagtaa
       61 attgctttac ataatataac cttttattta tacttcattt ataaatagta agttataaaa
      121 ggttcaaaaa tggcagaccc tatagaatgg tctattatca cgactgactg ctttgatgaa
      181 tggttcggtc aacaagatga aagtactcaa gaacgtgtct tggcttgttt aatctatctg
      241 cggaaattcg gttatagtct aggcagacct tatgctgata cagtttatca gtctaaattc
      301 cctaatatga aagagttacg cattcaaaac aaaggtaagc caattcgagc attttatgct
      361 tttgaccctt tacgccaagc gatagtactt tgtgctggcg agaaaggtaa agacaaaaga
      421 ttctatgaaa aaatgatcgc tcttgctgat gcggaattta cctcctattt aaataattta
      481 gagaaagaaa atgaaaacgt tagatcaaat gctcaatgaa ctctctcctg aacgccgcca
      541 aaaagtggaa gatatggctc aagaaatgat tagagagata caactttcaa aactaagaga
      601 agagctaaat ctctctcaac aacaattagc tacagctctc ggtatttcac agcctgctat
      661 tgctaaactt gaacagaaag ataacgatcc aagattatcc acgctcaaac gctatattga
      721 agcaatgggc ggtaagttaa aacttgccgt tgaattgcct acaggcgatg agcgtgtaat
      781 taggatttaa ttactttgtt aa
//
LOCUS       DANLXQ010000306          771 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.314, whole genome shotgun sequence.
ACCESSION   DANLXQ010000306 DANLXQ010000000
VERSION     DANLXQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.314"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            157..>771
                     /locus_tag="PXD94_002710"
     CDS             157..>771
                     /locus_tag="PXD94_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287503.1"
                     /translation="MSKNTHSYLPLQKYHILPIYGISPELIKKSVKDCSRDREKISHC
                     TLLNACSKALGFSGGFAGFQEDYLSNLTQFMEKHGLLYLVDLTKQKYPVNDMCMTRSL
                     ALKKQDISERIFFSGKEIPKRIFTGFNFRYDMHLANGFRIGNSLKTPYSDIGICEDIN
                     EALKNPSIKIDCRDGTIRSMADVVIGRYLDKVLFYYFNLIGDQLV"
BASE COUNT          276 a          109 c          124 g          262 t
ORIGIN      
        1 caggtatcca tacttgcctt gaacaaaaga gataattcga tagattatat tgcttatagt
       61 caattcattt ataaattaaa tccttttatt tcctagtatg gctgtgattt tcacagaggc
      121 atttttctaa cttaattaac aaagaggatt taatatatgt caaaaaacac ccatagctat
      181 ttacctttac aaaaatacca tattctacca atttatggta tttccccaga attaattaaa
      241 aaatctgtta aagattgttc tagagataga gaaaaaattt ctcactgtac tttgttaaat
      301 gcttgctcaa aagctttagg attttctgga ggctttgcag ggtttcaaga agattattta
      361 tctaatttga cgcaattcat ggaaaaacat gggctcttat atcttgtaga tttaacaaaa
      421 caaaaatatc cagtaaatga tatgtgcatg acaaggagct tagctctgaa aaagcaagat
      481 atatctgaaa gaattttctt tagcggaaag gaaatcccta agagaatatt tactggattt
      541 aatttccgat atgatatgca tttagctaat ggttttcgta tcggaaattc cttaaaaacc
      601 ccatattctg acataggtat atgtgaagac ataaatgaag cattaaaaaa tccatcaata
      661 aaaattgatt gccgtgatgg cacaatcaga agtatggcag atgtagtcat aggtagatat
      721 cttgataaag tattgtttta ttattttaac ttgataggtg atcagttagt c
//
LOCUS       DANLXQ010000307          768 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.315, whole genome shotgun sequence.
ACCESSION   DANLXQ010000307 DANLXQ010000000
VERSION     DANLXQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.315"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>768
                     /locus_tag="PXD94_002711"
     CDS             <1..>768
                     /locus_tag="PXD94_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6287504.1"
                     /translation="EYQKKCIANYFAFDQKIQLNTQTNQTLEAIAQAIFKSWFVDFDP
                     VRAKAAALSEGKSEHEANLAAMSVICGKDTSELNDTEYKALWQIAEAFPSEFGDEGLP
                     IGWKFNQADNLFDVGIGKTPPRKESEWFSDNANDTEWISIKDMGNQGLFITESSEYLK
                     AEAVDTFNIKRIPENTVILSFKLTVGRVSITTKETTTNEAIAHFKIPSSSNLSSEFLY
                     CYLKNFDFNNLGSTSSIATAVNSKMIKEMEILEPSVL"
BASE COUNT          280 a          141 c          143 g          204 t
ORIGIN      
        1 ttgaatatca aaaaaaatgt attgctaatt attttgcctt tgaccaaaaa atccaactca
       61 acacccaaac caaccaaacc ctagaagcca ttgcacaggc aatcttcaaa agctggtttg
      121 tggattttga ccctgtgcgc gccaaagccg ccgccctaag cgaaggcaaa agcgaacacg
      181 aagccaacct tgcggcaatg tcggtgattt gtggtaaaga caccagcgag ttaaacgaca
      241 ccgaatacaa agcgctttgg caaatcgccg aagccttccc aagtgagttt ggagatgaag
      301 ggttacctat tggctggaaa ttcaatcaag cagacaattt atttgatgtt ggtattggga
      361 aaaccccacc aagaaaagag agtgaatggt tttctgataa tgcaaatgat acagaatgga
      421 tttctattaa agatatggga aatcagggat tatttatcac agaaagtagc gaatatctaa
      481 aagctgaagc cgtagataca tttaatatta aaagaattcc tgaaaatact gtaattttga
      541 gttttaaatt aaccgttggt agagtttcaa ttacgacaaa agaaacaact actaacgaag
      601 ctattgctca ttttaaaatt cctagctcat caaatctaag ctcagagttt ttatactgct
      661 acttaaaaaa ttttgatttt aataatctag gaagtacatc atcaatcgca acggcagtca
      721 attcaaaaat gataaaagag atggaaattt tagaaccatc agttctag
//
LOCUS       DANLXQ010000308          742 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.316, whole genome shotgun sequence.
ACCESSION   DANLXQ010000308 DANLXQ010000000
VERSION     DANLXQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.316"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(69..707)
                     /locus_tag="PXD94_002712"
     CDS             complement(69..707)
                     /locus_tag="PXD94_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL6287505.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDVEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
BASE COUNT          235 a          150 c          113 g          244 t
ORIGIN      
        1 aataaattac aacgctcaaa ttttaacaat tttttgccaa aaaattaaga tatttaaccg
       61 cttgcacctt atattttaaa accttgtcca tcgttccaag aaaccgtata ttctagaggt
      121 aaacggcaac ttggcaacgc tgggtttgct gctttaccga tagctagtag cataacaggc
      181 aaataacgtt ctgcatctat attcaataat tttaatacag cagggcgctc aaacacgcct
      241 atcgcatgag tatcatagcc cttatctttg gcaattagca ttaattgcat tgccgccaaa
      301 ctagtatcaa ttaatacctg atctctaatt tctgcttgag ttgcttggtt atgaagattt
      361 agtaaaaaat caaatttacg ttcacgaaac tctttatgca aacacccagt ttctaccgat
      421 tgatcaacaa ttgcgcctaa taatttttca tattgcaaat ctgccaatat caccacaata
      481 gcagacgctg tttcacaagg tgggccatta aaggcaacat tttttgataa tgctgcttta
      541 agaatttcaa catcaataat gacaaaacgc caaggttgca gatttgcttt tgagggagca
      601 agctgggata aagcgaccat ttcttctaat tcagttctac taatcttaat tgttgaatta
      661 aaagctttga tagttttacg ctgttcaatc gtatgtttca attccatctc ttttctccta
      721 tctctcaaac aattgagata gt
//
LOCUS       DANLXQ010000309          740 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.317, whole genome shotgun sequence.
ACCESSION   DANLXQ010000309 DANLXQ010000000
VERSION     DANLXQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.317"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            7..678
                     /locus_tag="PXD94_002713"
     CDS             7..678
                     /locus_tag="PXD94_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL6287506.1"
                     /translation="MKKSTLAIILGGALLTGTAVAHQAGDVIFRAGAVHVKANSSSDA
                     KSKAATGIDVDLEVKNNTQLGLTATYMLADNVGIELLGATPFSHKINAKAKVLESGAE
                     VDLDRVVILKQLPPSLYAQYYFFELTAKVKPYVGAGLNYTRFYHAKSVSPAVTDLSVK
                     KHSFGPVANLGVDVKLTDNIYFNAAAWYTRIKTTAKFKAAGADHEVKVKLDPVVLFAG
                     FGVKF"
BASE COUNT          235 a          128 c          155 g          222 t
ORIGIN      
        1 atgaaaatga aaaaatcaac tttagcaatt attttaggtg gggcattatt aactggaact
       61 gcggtggcac atcaagcagg tgatgtgata ttccgtgccg gtgcggtaca tgttaaggca
      121 aattcaagtt cagatgcaaa atcaaaagca gcgacaggta ttgatgtaga tttagaagtg
      181 aaaaacaata ctcagttagg cttaactgca acttatatgt tagccgataa tgtgggtatt
      241 gagttattag gtgcaacccc tttctctcat aaaattaatg ccaaagccaa agttttagaa
      301 tcaggtgcag aagtagattt ggatagagtt gttatcttaa aacaattacc accgagcctt
      361 tatgcccaat actatttctt tgagctaact gcaaaagtaa aaccttacgt tggtgcaggt
      421 ttaaattaca ctcgcttcta ccacgctaaa tcagttagcc ctgctgttac tgacttaagt
      481 gtaaaaaaac attctttcgg accggtagca aacctaggtg ttgatgtaaa attaaccgat
      541 aacatctact tcaacgcggc agcttggtac acacgcatta aaacaaccgc aaaatttaaa
      601 gcagcaggtg cagatcacga agtaaaagta aaacttgatc cggttgtctt atttgccggt
      661 ttcggggtga agttctaatt atttgttggg aacgagttgg gctaatctta gtgattagtc
      721 cttttttgtt aaagttgttt
//
LOCUS       DANLXQ010000310          732 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.318, whole genome shotgun sequence.
ACCESSION   DANLXQ010000310 DANLXQ010000000
VERSION     DANLXQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.318"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(19..303)
                     /locus_tag="PXD94_002714"
     CDS             complement(19..303)
                     /locus_tag="PXD94_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287507.1"
                     /translation="MANITLSHITPKHFFETVRLLKMQEHRIYIGVDSIEVRFKQKRF
                     ESKKETDKRLEQLEHFMLFLQEIINSDIPIDLDEFIYTYHRSVRRGFYRR"
     gene            complement(321..482)
                     /locus_tag="PXD94_002715"
     CDS             complement(321..482)
                     /locus_tag="PXD94_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL6287508.1"
                     /translation="MEKIENSSNLVLKLSLENQMLILESLEHPKEPNLVMLEALKKYS
                     LYVAKTSNK"
BASE COUNT          232 a          127 c          109 g          264 t
ORIGIN      
        1 cactcctctt tcacatcact aacgtcgata aaaacctctc cttactgacc tgtgataggt
       61 gtatataaac tcatctaaat ctataggaat atctgaatta atgatttctt gtagaaagag
      121 cataaaatgc tctaactgtt ctagtctctt atctgtttcc ttttttgact caaaacgctt
      181 ttgcttaaaa cgaacttcta tcgaatctac accgatgtaa attcgatgtt cctgcatttt
      241 gagtagtcgg acagtttcaa aaaaatgttt aggggtgatg tgagaaaggg ttatatttgc
      301 catagtttag cttgaaaacg ctatttatta cttgttttag caacatacaa agaatacttt
      361 tttaaagcct ctagcattac taaattaggc tctttaggat gttctaagct ctcaagaatt
      421 agcatttgat tttcaagaga aagctttaga acaagatttg aactattctc tattttttcc
      481 attttaatta tctcattaga tgactttcgc ataacaacag gttatgtgta aattctcgtg
      541 cagtgggaca agcccgattg tactgcactc gaattgtaac ataacctgta ggacattatg
      601 cgaaaattaa taatataagt ttttcatcaa tcggcggtat tattgaatac gccatcacat
      661 ctatgtaaca gcattaatct ataagttatt atgtaaatac actcattttg taacaaatac
      721 ttttcatttt at
//
LOCUS       DANLXQ010000311          728 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.319, whole genome shotgun sequence.
ACCESSION   DANLXQ010000311 DANLXQ010000000
VERSION     DANLXQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.319"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>728
                     /locus_tag="PXD94_002716"
     CDS             <1..>728
                     /locus_tag="PXD94_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287509.1"
                     /translation="KQPVMQSAFNHQKRIVKWLWAEFWDKNIISMTLRIDGYRNANLW
                     HYLRYQSRIEVLYKIANEHPNLLPLLAYVKPEYWKCSDLFSYQNWVRSRDKQPFILYA
                     KCQDKMLCESKGQWRWLKKQSRIVVDQCFLHVNQWQLLTEIQMPFKIPVCVICFILTN
                     FSMISCELDRSRQRRLLSIFIQYIYHYWKDNGFRQLKIHLSHLRVLFQDIEDYMIHEG
                     YLHGIPQTWQHLVYHLNLINSNTS"
BASE COUNT          248 a          107 c          131 g          242 t
ORIGIN      
        1 aagcaacctg ttatgcaaag tgcatttaat catcaaaaaa ggattgttaa gtggttatgg
       61 gctgaatttt gggataagaa tattatctcg atgacactta gaattgatgg ttatagaaat
      121 gctaacttat ggcattattt acgttatcaa tcacggattg aggtattgta taaaatagca
      181 aatgaacacc cgaatctact accattatta gcctatgtta aacctgagta ctggaaatgc
      241 tcggacttat ttagttatca aaattgggta agaagccgtg ataaacagcc ttttattttg
      301 tatgctaaat gccaagataa gatgttatgt gaatcaaagg ggcaatggag atggttaaaa
      361 aaacagtcga gaatagttgt agatcaatgt tttctacatg ttaatcaatg gcagttatta
      421 acagaaattc aaatgccttt caagatccca gtatgtgtca tatgtttcat acttactaat
      481 tttagcatga tcagctgtga gttagatcga tctagacaac gtcgattact ctctattttt
      541 attcaatata tctatcatta ttggaaagat aatgggttta gacaattaaa gatacatctt
      601 tctcatctga gagtgctttt tcaagatatt gaagattaca tgatacatga ggggtattta
      661 catggtatac ctcagacttg gcaacatttg gtttaccacc tcaaccttat aaattcaaat
      721 acatcaaa
//
LOCUS       DANLXQ010000312          710 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.320, whole genome shotgun sequence.
ACCESSION   DANLXQ010000312 DANLXQ010000000
VERSION     DANLXQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.320"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            48..>710
                     /locus_tag="PXD94_002717"
     CDS             48..>710
                     /locus_tag="PXD94_002717"
                     /inference="COORDINATES: protein motif:HMM:NF012920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL6287510.1"
                     /translation="MNELRLLRQKKLKRLIDEVSEGNIADFARKIGKEPSYVARMLYP
                     SDKAGAKPIGEKMALHICHKFVLPLDWFNRDDDGEFIQINNGEEDNIILSVLNIQASA
                     GIGAKNGDVMEVIREIKFNPEQFYNLFRGITPEHIELISVKGDSMEPTFSHGDLLFVD
                     VTIQEYDGDGVYVFTCDDYIFVKRIQKTGRHFTILSDNDRYKPWQLDQEIYIHGKVKV
                     HQS"
BASE COUNT          251 a          105 c          147 g          207 t
ORIGIN      
        1 gtaaataaga gtacaattac taaaaagtaa tccttagagg aggttatatg aatgagttga
       61 gacttcttag acaaaaaaaa ttgaaacgcc taattgatga agtgagtgag ggaaatattg
      121 ctgactttgc aagaaaaata ggtaaagagc ctagctatgt agcaagaatg ttgtatccgt
      181 ccgataaagc tggagctaag cctattggtg aaaaaatggc actccatatt tgccataaat
      241 ttgtgctacc ccttgattgg tttaatcgag atgatgacgg cgaatttatt caaatcaaca
      301 atggtgagga agataatatc atcttaagtg tattgaatat tcaagcatcg gccggtatag
      361 gagcaaaaaa cggtgatgtg atggaagtca ttagagaaat taaattcaat ccagaacaat
      421 tttataacct atttagaggt attacgcctg aacatatcga gctgatcagt gtaaaaggcg
      481 attctatgga gccaacattc tcacacggtg atttgctttt tgttgatgta actatccaag
      541 aatacgatgg cgatggtgtg tatgttttta cttgcgatga ctatattttt gtaaaaagga
      601 ttcaaaaaac aggcagacac tttactattt tatctgataa tgaccgctat aaaccttggc
      661 aattagatca agaaatttat attcacggca aagttaaagt ccaccaaagc
//
LOCUS       DANLXQ010000313          697 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.321, whole genome shotgun sequence.
ACCESSION   DANLXQ010000313 DANLXQ010000000
VERSION     DANLXQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.321"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..294
                     /locus_tag="PXD94_002718"
     CDS             <1..294
                     /locus_tag="PXD94_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287511.1"
                     /translation="DTRYNKNGQGAVSERNIIDAKNRFKASHPYAKIISCVRGSETYV
                     TRPAKANATTKSVKEKQSQPQESSSLSSMLLGAAVTAGVGFVANKWFSKKDNT"
     gene            322..>697
                     /locus_tag="PXD94_002719"
     CDS             322..>697
                     /locus_tag="PXD94_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287512.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKAYVQENFPNVLENPFLFQTLSAASHNELKINRGY
                     KFKEKMLDEFYNLIELSEKDDEE"
BASE COUNT          260 a          108 c          128 g          201 t
ORIGIN      
        1 gataccagat ataacaagaa tgggcaaggt gctgtttctg aaagaaatat tatagatgct
       61 aaaaatcgtt ttaaagcgag ccatccatac gcaaaaatta tttcttgtgt tagagggtct
      121 gagacatatg taacacgtcc agcaaaagct aatgctacaa caaaatctgt caaggaaaag
      181 caatctcaac cacaagaaag ttcatcgctc agttcaatgt tacttggtgc ggctgtaact
      241 gctggggttg gttttgtagc caataaatgg tttagcaaaa aagataacac ttaatttatc
      301 tctaaccaat gtggagtaaa aatggctgga ttattaccac cttatttcaa aaagaaaaat
      361 agtgatcact ataaagatgc tgtaaaacaa tttgggaaag aacaagttga tgaggttata
      421 gcacagtgtg atgaacttga atcttgggta aaagaaatgc caacaaaaca acttattatg
      481 tatgttggtt gtttccatac ttttaacaaa ttatctcaat ctaaagctta tgttcaagag
      541 aactttccaa acgtactgga aaacccgttt cttttccaaa cactttcggc agcgtctcat
      601 aatgaactta aaataaatcg aggatataaa ttcaaagaaa aaatgcttga tgaattctat
      661 aatcttatag agttgtctga aaaggatgat gaagaat
//
LOCUS       DANLXQ010000314          649 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.322, whole genome shotgun sequence.
ACCESSION   DANLXQ010000314 DANLXQ010000000
VERSION     DANLXQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.322"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..624
                     /locus_tag="PXD94_002720"
     CDS             <1..624
                     /locus_tag="PXD94_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL6287513.1"
                     /translation="NTVLNTLQCDYVLFSSEDNYQVIINLKKADYPKNEANFITMTLN
                     KKFGDAKFSGANHYLRCASFFNKKSTNNNEKSVLVDFTNTKTEEDNKCYFDNLLSSYK
                     NNNVKLEPLDIKIIDELGDDKAVIAQKEIQAEIALCKRIFKQLDWSAVDFRIVKRLYR
                     KGFSENEIAVALVRFTDFEDRHCDSHDYLTRTITKAIQNYQQCSKAC"
BASE COUNT          259 a           98 c          100 g          192 t
ORIGIN      
        1 aacacagttt taaacacact acaatgcgat tatgtgctgt tttcttctga agacaactac
       61 caagtcatca tcaatctaaa aaaagctgat taccccaaaa atgaagcaaa tttcattaca
      121 atgactttaa acaagaaatt tggtgatgca aaattttccg gtgcaaacca ttacttaaga
      181 tgtgccagct tcttcaacaa gaaaagcaca aacaacaatg aaaaatcagt tttagttgat
      241 tttacaaaca caaagacaga agaagacaat aaatgttatt ttgataactt attatctagt
      301 tataaaaata acaatgtaaa acttgagcct ttagacatca aaattattga tgagcttgga
      361 gatgataaag cagttattgc acaaaaagaa attcaagcag aaattgcttt gtgtaaacgt
      421 atattcaaac aattagattg gtctgctgtt gattttagaa ttgttaagcg tctgtacagg
      481 aaaggatttt cagaaaatga aattgctgtt gcacttgtca gatttactga ttttgaagac
      541 agacattgtg attcacatga ttatttgaca agaacaatca caaaagccat tcaaaactat
      601 caacaatgct caaaagcttg ttagatgtta taaggaaagt taaaaatca
//
LOCUS       DANLXQ010000315          648 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.323, whole genome shotgun sequence.
ACCESSION   DANLXQ010000315 DANLXQ010000000
VERSION     DANLXQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.323"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>648
                     /locus_tag="PXD94_002721"
     CDS             <1..>648
                     /locus_tag="PXD94_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL6287514.1"
                     /translation="LLSLEGFKMKVVHNGLEGLNELESNQYDLVLLDVMMPVLSGVKT
                     LKRIRQKYSLPVLMLSARDDQIDRILGLELGADDYVPKPFNNRELVARMKAILRRTTS
                     RPASTDHSEQNYTENISKSLQFAGVELHSGRQQAYYQGCDLDLTGTEFALLQMLMRNS
                     GEILSRELLSLKILGKNLTPYDRAIDMHISNLRKKLPEREDGLPWFKTLRGRGYL"
BASE COUNT          223 a           94 c          129 g          202 t
ORIGIN      
        1 attactttca ttagaaggat ttaaaatgaa agttgtgcat aatggtttag aaggcttaaa
       61 tgagttagag tctaatcaat atgatttagt attactagat gtgatgatgc cggtattaag
      121 tggcgtgaaa acattaaaac gtatacgaca aaaatactca ttacccgttt taatgttaag
      181 tgcaagagat gatcaaattg atagaatctt aggtttagaa ttaggggctg acgattatgt
      241 accgaaacct tttaataatc gtgaactagt cgctagaatg aaagcgatac ttcgaagaac
      301 aacctctcgc ccagcttcaa cagaccattc tgaacagaat tatactgaaa acatctctaa
      361 atcattacaa tttgccggag ttgaattaca ttcaggacgt cagcaggctt attatcaagg
      421 atgtgatctt gatttaacgg ggactgaatt tgcattatta caaatgttaa tgcgaaactc
      481 aggggagatt ttgtccagag aattattgag tttaaaaatt ttagggaaaa atttaacgcc
      541 ttatgatcgt gcaattgata tgcatatttc taatcttcgt aaaaaattac ccgaacggga
      601 agatggatta ccttggttta aaaccttaag aggtcggggt tacttatt
//
LOCUS       DANLXQ010000316          644 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.324, whole genome shotgun sequence.
ACCESSION   DANLXQ010000316 DANLXQ010000000
VERSION     DANLXQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.324"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>644
                     /locus_tag="PXD94_002722"
     CDS             <1..>644
                     /locus_tag="PXD94_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL6287515.1"
                     /translation="EIEQTRAAYDRLKRSGVASQNELRRASEATKQRVRELNAELGKT
                     SFGDKAKGFGRGLMSAGVGIAAGAAMARQPAKNQMEFDRQLAMASNTAFSDRDVKGRI
                     EGKKELLAAVQEAVKVGGGTKEDALLTLDTLIASGTMKAETAMSLLPTLQKGAVATGA
                     SSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGNFELADMARWLPQQMAAG"
BASE COUNT          184 a          129 c          190 g          141 t
ORIGIN      
        1 gaaattgaac aaactcgtgc agcctatgac cgcttaaaac gctcaggtgt tgcctctcaa
       61 aatgagctac gccgtgcctc agaagccact aagcaacggg tgcgagagct gaatgcagaa
      121 ttgggtaaga caagctttgg ggataaggct aaaggatttg ggcgtggttt gatgagtgcc
      181 ggtgtcggta ttgcagcagg tgctgcaatg gctcgtcagc cagccaagaa ccaaatggag
      241 tttgaccgac agttagcgat ggcatcaaac actgcctttt ccgaccgtga tgttaaaggg
      301 cgtattgagg gcaagaaaga gttattagca gcggtgcaag aggcggttaa ggttgggggt
      361 ggaacaaaag aagatgctct tttaactctt gatacgctaa ttgcctcagg aacgatgaag
      421 gctgaaactg caatgagcct attaccaacc ttgcagaaag gtgctgtagc aacaggtgca
      481 agctccgagg atatggcaaa aattgcgatc tcctcaatgc aacaatttgg gattaaagaa
      541 gaggatattg ggcgggcatt ggatatggca gttgcagccg ggcaagcagg taactttgag
      601 cttgcagata tggctcgctg gctaccacaa caaatggccg cagg
//
LOCUS       DANLXQ010000317          643 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.325, whole genome shotgun sequence.
ACCESSION   DANLXQ010000317 DANLXQ010000000
VERSION     DANLXQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.325"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..493
                     /locus_tag="PXD94_002723"
     CDS             <1..493
                     /locus_tag="PXD94_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287516.1"
                     /translation="ARPLNTLEVKGLTVVDDSQKPLFSEVNQALYHGLSPIEVVASRV
                     QITRAITTYTKNVTNTDDPSYLDLTTIRTLDYVRKAIQTRQRLRFPRAKNSHRIVAKV
                     RSEILDVLYQLEGEEVIENVEAWKNRLLVVRNQDPTYLDLEIPADVVNGLHVIRNKIT
                     LIL"
     gene            504..>643
                     /locus_tag="PXD94_002724"
     CDS             504..>643
                     /locus_tag="PXD94_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287517.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYS"
BASE COUNT          182 a          127 c          160 g          174 t
ORIGIN      
        1 ggctcgcccg ttaaataccc ttgaagttaa aggcttaacg gtcgtagatg actcgcagaa
       61 accgttattt agcgaagtaa accaagcgtt atatcacggt ttaagcccga ttgaggtggt
      121 ggcaagccgt gtgcagatta cccgtgcgat tactacatac accaaaaacg tgacgaatac
      181 tgatgaccca agctatttgg atttaaccac cattcgtacg cttgactatg tgcgtaaggc
      241 aatccaaacc cgtcagcgtt tgcgtttccc tcgtgcgaaa aactcccacc gtattgtggc
      301 aaaagtacgc tcggagattt tagatgtgct ttatcagctt gagggcgaag aggtaatcga
      361 aaatgtggaa gcgtggaaaa atcgcttatt agtggtgcgt aatcaagacc cgacttactt
      421 ggatttagaa atccctgcgg atgtggttaa tggcttgcac gttattcgta acaaaattac
      481 gttaatttta tagggggtgc tagatgtcaa ttgtttatga aggctcttgc gtgcttgagg
      541 tagatggcgt tgagattgaa attaccaaac ttgatgtaaa aaccgtaacc ggtcgcaagc
      601 tggttaaaac gatgaacagt tccggacgtg caagaggtta ttc
//
LOCUS       DANLXQ010000318          643 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.326, whole genome shotgun sequence.
ACCESSION   DANLXQ010000318 DANLXQ010000000
VERSION     DANLXQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.326"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            10..636
                     /locus_tag="PXD94_002725"
     CDS             10..636
                     /locus_tag="PXD94_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL6287518.1"
                     /translation="MTKIKFYGNLKKFGSEFSLEVKDTAEAIRALCTQISGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFNFVLGAALIGAA
                     FFTGGASIAAWGAGAKMMGMLGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSNL
                     NNLVTQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
BASE COUNT          199 a          116 c          157 g          171 t
ORIGIN      
        1 tggtgagtca tgactaaaat taaattttac ggcaatttaa aaaaatttgg tagtgaattt
       61 agcttagaag ttaaagatac tgccgaagct atccgagcct tatgtacaca gattagcgga
      121 ttgcgtgagg cattacgaga cggtgtttat aaagtccgta tcggcaaaca gtatttagac
      181 ccgtcagccc ttgaaaaagg gcttttttat tgtctgaaga aagggcaaac cattcatttt
      241 acccctgtta tcaaaggagc gaaaagcggt ggcgtgttta attttgtttt aggtgcagcc
      301 ttgatcggag ccgcattttt tactggcggt gcttctattg cggcttgggg agctggagca
      361 aaaatgatgg gtatgttagg ggcttcgatg ttactaggag gagtttccca aatgctcacc
      421 aaaatgccaa aagcccccac aatgggtaat gaaacagaaa aagaaagctc aacggctttt
      481 tcaaatttaa ataacttggt gactcaaggc aaacctgtgc cgttggctta tggcttaatt
      541 cggaccggct cgcttgtgat atcgcaggga gttgagacga tcaccattaa agaaaaccaa
      601 ccggcaagca acaagaaaac aggctttaga aaataaggga taa
//
LOCUS       DANLXQ010000319          629 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.327, whole genome shotgun sequence.
ACCESSION   DANLXQ010000319 DANLXQ010000000
VERSION     DANLXQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.327"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            15..515
                     /locus_tag="PXD94_002726"
     CDS             15..515
                     /locus_tag="PXD94_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL6287519.1"
                     /translation="MQTINEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQ
                     NGATYLRIAKKDIEYIRQYPKTCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGI
                     IEELRTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLIL
                     HRRLTV"
BASE COUNT          248 a          109 c           98 g          174 t
ORIGIN      
        1 atcggagtac atatatgcaa acaatcaatg aaattaaaga agaaatctac cctatatttg
       61 agcctatcat tggaacaatt cggctaaaaa cctacttttc ctactacgca gtttttaagg
      121 atggattgat gattgcattg tatcaaaatg gagcaactta tttacgtatt gcgaaaaaag
      181 atattgaata cattcgtcag tatcctaaaa cctgtatctt atgcgatgaa aaaattggta
      241 tacaaagtag aaaatttcat tatattccgc aagttctcat agatgataca ccaaaattta
      301 gctcgctact ctttggaatt atagaggaac tccgaacgga gaagtacaaa ataagccaaa
      361 agcgagctac acaaatccga aacctaccaa atatgaattt aaagttagaa agaatgttga
      421 aaaaaatagg gattcattcc attcatgatt ttactcaaga agggtatatg tcaactttta
      481 tcaaactgat tctccaccga agacttactg tttaagctaa acggagcttt aaatcaccaa
      541 tatatttaca ccttcacaga tcaacaaaaa agggaactaa tgcaagaagc aaatcaagcg
      601 ctatataatt caggcctaag aaaaagatt
//
LOCUS       DANLXQ010000320          620 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.328, whole genome shotgun sequence.
ACCESSION   DANLXQ010000320 DANLXQ010000000
VERSION     DANLXQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.328"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            15..536
                     /locus_tag="PXD94_002727"
     CDS             15..536
                     /locus_tag="PXD94_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL6287520.1"
                     /translation="MSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGLIGLYHMLENG
                     KESLVRVYKANDYFGFRTFFGDNRYHCSARVLQPAELIRITPLNFIEFWSNNSALIIS
                     LLKQLSTELQDAEHRLSMVSYQKTQERVMNSIHYLHQRYPNYHWTNREIAEFAGCETE
                     TAIRLRRELKKIS"
BASE COUNT          200 a          114 c          115 g          191 t
ORIGIN      
        1 cccccgaaca attgatgtct tgccaattaa gtgtaatgtc tgatctagaa aaaggagtcg
       61 tcatttattc tcagggttat cacgccagag aattttattt tttaaaaagc ggattaatag
      121 ggctgtacca tatgcttgaa aatggtaaag agagcttggt gagggtatat aaagccaacg
      181 attattttgg gtttagaacc ttttttggcg ataatcgcta ccattgctcg gctcgggtgt
      241 tacagccggc agaattgata cgtattaccc ccttaaattt cattgagttt tggtcgaata
      301 attccgcctt aattatctct ctgttaaaac agctctcgac agaattacaa gacgcagagc
      361 atcgtttatc aatggtttcc tatcaaaaaa cgcaagagcg ggtaatgaac agtattcatt
      421 atttacacca acgttatccc aattatcact ggactaaccg tgaaatcgcc gaatttgcag
      481 gttgtgaaac cgaaacagcc attcgattaa ggcgagaatt aaagaaaatc agttaaattg
      541 tcataaatta ttcatagttg tgatcaccat cacatatcta ttatgacaaa catcatattc
      601 tatcgttaaa aataagattt
//
LOCUS       DANLXQ010000321          613 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.329, whole genome shotgun sequence.
ACCESSION   DANLXQ010000321 DANLXQ010000000
VERSION     DANLXQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.329"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..504
                     /locus_tag="PXD94_002728"
     CDS             <1..504
                     /locus_tag="PXD94_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL6287521.1"
                     /translation="FILAIIMTLRCTGIRRGQLVKLKIGDVNLSNRTIYISPEINKNH
                     DYHIVPISDSLYPHIERLIQELKIHKQTDDCQLFNLNLFSRYTRRKGQPMSQNQLTHI
                     FRHISDLVGFTSSPHRFRHTVATQLMKKPENVYVVKKLLGHKDISVTLGYIEYDVEML
                     RDTVNLL"
BASE COUNT          215 a           96 c          107 g          195 t
ORIGIN      
        1 tttattcttg ctatcattat gacattgaga tgcacaggaa ttaggagagg gcaattggtt
       61 aaactgaaaa tcggtgatgt taatttatca aataggacga tctatatttc accagaaatt
      121 aataaaaacc atgattatca tatagtaccg atatccgatt cactttatcc ccatatagaa
      181 aggctgattc aggagttaaa gatccataaa caaacggatg attgccagtt atttaacctc
      241 aatttatttt ctcgttatac tcgtaggaaa ggtcagccga tgagccaaaa ccagcttact
      301 catatattta ggcatatttc cgatttggtt ggcttcacgt catcaccaca tagatttcgg
      361 catacagtag ctactcaatt gatgaagaaa cctgaaaatg tttatgtagt taagaaatta
      421 ttaggtcata aagatatcag cgttacatta ggatatattg aatacgatgt ggaaatgcta
      481 cgagacaccg tgaacttact ctaataaaag agaaatgaga gaaaactatt ctctcattta
      541 ttgtgataaa taggaaactt ctgtcttatt ttcagatgaa ttgattttga gaactttatc
      601 aaaatataag cgt
//
LOCUS       DANLXQ010000322          611 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.330, whole genome shotgun sequence.
ACCESSION   DANLXQ010000322 DANLXQ010000000
VERSION     DANLXQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.330"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..453
                     /locus_tag="PXD94_002729"
     CDS             <1..453
                     /locus_tag="PXD94_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287522.1"
                     /translation="PMLEEASDNVTDITQPSPWQNAGVTAIHGGSIVTNTVTAQQIAA
                     NTITANEIAAGTIAARNMAANSINASHIVGSSITADKLNVNNLAAISANLGAITAGSI
                     NINNRFKVSNAGVVEMRANSGNVGMVMNNNSIIVYDEQGRVRVKMGKL"
BASE COUNT          208 a          100 c          129 g          174 t
ORIGIN      
        1 ccaatgcttg aggaggctag tgataatgtt acagatatca ctcagcctag cccttggcaa
       61 aatgccggtg taaccgctat ccacggtggc tcaatagtta ctaataccgt tactgctcaa
      121 caaattgcag caaataccat taccgccaac gaaatcgcag ccggcacgat tgcagctcga
      181 aatatggcag caaactcgat taatgcaagt catattgtag ggagttcgat cactgcggat
      241 aagctaaatg taaacaattt agcggcaata tcagcgaatc tcggagctat tacagctggt
      301 tctattaata tcaataatag attcaaggta agtaatgctg gagtagtcga aatgcgagcc
      361 aatagtggaa atgttggtat ggtaatgaat aacaatagta ttattgttta tgatgaacaa
      421 ggtagggtta gagtaaaaat ggggaaatta tgattctaat aggtatttta tgtattattg
      481 cattagcagt cttagggctg cttttttatt tccataaaaa aaaacgaaaa aaagtaaata
      541 aaggggtaaa tatggctgaa tatggtattc aaacttttga taccgctaat gtctctacat
      601 ttagtaccaa t
//
LOCUS       DANLXQ010000323          597 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.331, whole genome shotgun sequence.
ACCESSION   DANLXQ010000323 DANLXQ010000000
VERSION     DANLXQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.331"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
BASE COUNT          199 a           82 c          109 g          207 t
ORIGIN      
        1 aaactaagtt aaatgaattt tccttaaatc aaaatatagg ccgttatttt aagcaagaag
       61 tagggattac actccactat aaaggggtat caagttcggt acatgctggc tttacaaaag
      121 gaaatacatt acaagaacaa aaattcattt cactcaaagt tggatatgag tggtaagctt
      181 tatgtaacct gaagctacac ttgaatcggt tcaaacataa tgttactaaa aaagccttga
      241 aacattgatt ttcaaggctt tttaggtatc ggttgaaccg tataggatta taatttggtg
      301 ctggggtcat ctgaattgta tcacttcaag cattgataaa taagggtttt ggttaattca
      361 aattttattt gtactcctaa atatactcct aaaattcaaa gtgccttatt gcttaggcta
      421 ttttgtgtac aattattcgg atcgtgtata gggaaattta gttcgtagtt ttctatagta
      481 gtttaagttt ataaagaaaa aatttaaaac tttttgaaat tttacccact ttacccactg
      541 atttgttgga agccttgata tataaggctt tatagccagt gggaagcaag tgggtta
//
LOCUS       DANLXQ010000324          593 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.332, whole genome shotgun sequence.
ACCESSION   DANLXQ010000324 DANLXQ010000000
VERSION     DANLXQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.332"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>593)
                     /locus_tag="PXD94_002730"
     CDS             complement(<1..>593)
                     /locus_tag="PXD94_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287523.1"
                     /translation="RILVDAYYEDGTTGGGEFVADLQDLVTPDDGGVCFVVNNNGGRW
                     KRVDLSHLTLFDFWAVGDGVTNDESAFVNAMRYSQFFIENGTFRINNAVNSVRDNVKI
                     LGNKTGKLVLGAGIQQAGAEVFNINHSNYFISGFCIETPNKAIGIRFKSLDDAGVKNL
                     HIDNVVFNGTFYGVRAGESIQADTNYPTDNVIVQNCQS"
BASE COUNT          193 a          150 c           89 g          161 t
ORIGIN      
        1 gattggcagt tttgtacaat cacattatca gttgggtaat tagtatccgc ctgaatactt
       61 tcacctgccc tgaccccgta gaaggtgcca ttaaacacaa cattgtcaat atggagattt
      121 ttaacaccag catcatccaa agacttaaat cgaataccaa ttgctttgtt cggtgtttca
      181 atacaaaaac ccgagataaa ataattgctg tgattaatat taaaaacttc ggcccctgct
      241 tgctggatac ctgcacctaa tactaatttg ccggtcttat tgcccaaaat tttcacatta
      301 tcgcgaacag aattaacggc attgttgata cgaaatgtac cgttttcgat aaaaaattga
      361 gaatatctca tagcatttac aaatgctgat tcatcatttg ttaccccgtc accgacggcc
      421 caaaaatcaa ataatgtaag atgtgaaagg tcaacacgct tccaccgccc cccattatta
      481 ttcacaacaa aacaaacccc accatcatca ggcgtaacta aatcctgcaa atcagccaca
      541 aactccccac cgcctgttgt gccgtcctca tagtaagcat ccaccaaaat acg
//
LOCUS       DANLXQ010000325          591 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.333, whole genome shotgun sequence.
ACCESSION   DANLXQ010000325 DANLXQ010000000
VERSION     DANLXQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.333"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
BASE COUNT          198 a          117 c           83 g          193 t
ORIGIN      
        1 tactttaatt gcatcttcag aaagtaaatc acaacgaggt aatacttcat tgatagcctt
       61 ccaagaaagt aaatcttgct actctatagc acctttcatt aaaagtggtt gtttctcaga
      121 ataactttca cgaaattctt gataagaaat tggaaattga attttcattt tcatctccac
      181 tagccttgat aatctgtacc atcaccacaa tcgtagtttg agccagattg gttattattc
      241 gttctacctg gaactccaaa cgtaggcttt attggtaaag atgatggaat taattctttt
      301 acaccgctag gtaaagaatt ttcatgtatt gtaataggta ggctcctcgc taaatcaagg
      361 gctaaattaa cagaagtact ggataaaccg cctaaagccc ccctcttgca aaccctgtag
      421 caaaagcaac gccataagat acaagatctg gttgattagc cttattttca gaattccctc
      481 caactactaa gcttaattgt aaaattgatg gttctttcat ttcattctcc ctttaatcta
      541 attctgatca caaaaaccaa tattaaaaat aattctaaaa ataatactat a
//
LOCUS       DANLXQ010000326          590 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.334, whole genome shotgun sequence.
ACCESSION   DANLXQ010000326 DANLXQ010000000
VERSION     DANLXQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.334"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..320
                     /locus_tag="PXD94_002731"
     CDS             <1..320
                     /locus_tag="PXD94_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287524.1"
                     /translation="YLEEYNDLGTIRYKFQEENLYGFLRDGATLVANGIVNEPSVDCF
                     SQEIAQFTGCHIFSSLYIAFNTQRSFKSHWDSRDIFAVQMQGKKRWIIHTPTFKKTIV
                     YAS"
     gene            310..>590
                     /locus_tag="PXD94_002732"
     CDS             310..>590
                     /locus_tag="PXD94_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL6287525.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLA"
BASE COUNT          183 a           84 c          117 g          206 t
ORIGIN      
        1 attatttaga agaatataat gatctaggta ctattcgtta taaatttcaa gaagaaaatt
       61 tatatggttt tttaagagat ggggcaacat tagtcgcaaa tggtattgta aatgaaccaa
      121 gtgttgattg cttctctcaa gagatagctc aatttacagg ctgtcatatt ttttcaagtt
      181 tatatattgc gtttaatact caaagatctt ttaaaagcca ttgggatagt cgtgatattt
      241 ttgcagttca aatgcagggt aaaaagcgtt ggattattca cactccaaca tttaaaaaaa
      301 ccattgttta tgcatcgtag taaagatatg cctgaatatg ctcctaatct tgatgatgta
      361 tatatggata ttgtattaga agctggagat gttctttatt tacctcgtgg ctggtggcac
      421 gatcctattc ctgttggaga agaaactgtg catttagctg taggcatttt tcctgcttat
      481 actcataact atctgacttg ggtatctcaa aatatggttg agaaagaaat tgcccgagct
      541 tctttatcgc attatgagtc agataaagag ttgatagcac agttggcaga
//
LOCUS       DANLXQ010000327          589 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.335, whole genome shotgun sequence.
ACCESSION   DANLXQ010000327 DANLXQ010000000
VERSION     DANLXQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.335"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>589
                     /locus_tag="PXD94_002733"
     CDS             <1..>589
                     /locus_tag="PXD94_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL6287526.1"
                     /translation="INQKFLKFQWKSNYAYANWLETTANKIKRSKDDSIEWHRKNEKE
                     IPFWVITETWDFGALSKFYSLLNGQHQRKIAKKFNVDENTLKNWLNEINIVRNLSAHH
                     SRIWNRKFSDIAIPKLDDPRWSKFKKMQQYFSNLSLDDKAKERIFGRVAILWFLVSQT
                     STNYHWLDKFEDLLNDFPNLPNTKVELMGFNREILE"
BASE COUNT          218 a           76 c          111 g          184 t
ORIGIN      
        1 gattaatcaa aagttcttaa aatttcaatg gaaatctaat tatgcttatg caaattggct
       61 agaaactaca gctaataaaa ttaagcgtag taaagatgat tctattgaat ggcatagaaa
      121 aaatgagaag gaaatccctt tttgggtaat tactgaaacc tgggattttg gtgcattatc
      181 taagttttac tcattgttaa atgggcaaca ccaaaggaaa attgctaaga aatttaatgt
      241 agatgagaat acgttaaaga attggttgaa tgaaataaac attgtaagaa acctttctgc
      301 tcaccattct cgtatttgga acagaaaatt tagcgatatt gctattccta agctagatga
      361 tcctcgttgg tcgaaattta aaaaaatgca gcagtatttt tcaaacttat cgctggatga
      421 caaagcaaag gagagaattt tcggaagggt agctattctg tggtttttag ttagtcaaac
      481 cagcactaat taccattggt tagataagtt tgaagattta ttaaatgact tccctaattt
      541 accgaataca aaagtagaac tgatgggatt taacagagaa atattagag
//
LOCUS       DANLXQ010000328          587 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.336, whole genome shotgun sequence.
ACCESSION   DANLXQ010000328 DANLXQ010000000
VERSION     DANLXQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.336"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>587)
                     /locus_tag="PXD94_002734"
     CDS             complement(<1..>587)
                     /locus_tag="PXD94_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287527.1"
                     /translation="KYLMLCSLWFLSACFNESYDFRGKEFEEPVQAILVESDKIYFIG
                     STIDAIFDKTLIDNTKASDVIYFLNKLLQSPYGRKISNHLITLAIYDENIQIQYSPQF
                     IAKNLTMSDIQALQKLGFQFSHSKEYLWKSYAGLGGKITQFKNRKEILEKYKFKQPII
                     AKAKSYQKSIEFSFDDTISVIAAPITIPASIILFV"
BASE COUNT          187 a           92 c           94 g          214 t
ORIGIN      
        1 tacaaataaa ataatagatg cgggaatcgt aattggtgct gcaatgaccg aaatggtgtc
       61 atcaaagcta aattctatgc ttttctgata acttttcgct ttagcgatta taggttgctt
      121 aaatttatac ttttctagta tttccttgcg atttttaaac tgtgtgattt taccaccaag
      181 acctgcatag cttttccata gatattcttt tgaatggcta aattgaaaac ctaacttttg
      241 tagagcttga atatcgctca tggtgagatt ttttgcaata aattgcggac tgtactgaat
      301 ttgaatattt tcatcataaa tggctaaagt aattaggtga ttactgatct tccttccata
      361 cggactttga agaagtttat tcaaaaaata aataacatct gaagcttttg tattatctat
      421 cagcgtttta tcaaatatcg catcaattgt ggaaccaatg aaataaattt tatctgattc
      481 aaccaaaatt gcttgtacag gttcttcaaa ttcttttcct ctaaaatcat aactttcgtt
      541 aaagcatgcg gagagaaacc atagtgagca gagcattaag tattttt
//
LOCUS       DANLXQ010000329          564 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.337, whole genome shotgun sequence.
ACCESSION   DANLXQ010000329 DANLXQ010000000
VERSION     DANLXQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.337"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..543)
                     /locus_tag="PXD94_002735"
     CDS             complement(<1..543)
                     /locus_tag="PXD94_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6287528.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIAPFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECNYQLGGVKGVLSINLNDCQ
                     HIDTNCYFIRSEIARLISTAW"
BASE COUNT          207 a          117 c           74 g          166 t
ORIGIN      
        1 ccaagccgta ctgataaggc gagctatctc actacgaata aaatagcaat tcgtatcaat
       61 atgctgacaa tcattcagat ttattgataa aacaccttta acaccaccta attgataatt
      121 acattctaat ggttcttgat aaacgcaatt atattctccc aacgattcaa tcgtatcaat
      181 acacaaaaac tcgccatcta aatcataaaa attacgtaaa gagtaagcat aatcagattg
      241 catttctaat aaagcatgca caccagattc aacgtgagta ggttcaaacc aattatcatc
      301 atccagataa caaataatat cttctttcac taaaaatggt gcaattgcat taatgctgct
      361 atttacccaa ccatcagccc cggtattcat tgggagataa gtaacaataa cacttggaaa
      421 attctttaaa atatcagccg cttgttggtg atatttctct ccatcaacaa aaacataatg
      481 tttacaagga taagtctgat tttgtacgct taaaacagct ctttctaatt caggacgacc
      541 aattgttgaa ctgataacag ctac
//
LOCUS       DANLXQ010000330          554 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.338, whole genome shotgun sequence.
ACCESSION   DANLXQ010000330 DANLXQ010000000
VERSION     DANLXQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.338"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>554)
                     /locus_tag="PXD94_002736"
     CDS             complement(<1..>554)
                     /locus_tag="PXD94_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL6287529.1"
                     /translation="DLGITIKSPEQAATYVNDERYRRFNTLIDKKFNDSVLIELLNCF
                     EKRDDKRIEELVTDEAAIPTIFEYILGIIWYKVSERQGNILDFMKLSLEANLLPKTHA
                     AGGYADIIYEYEACTSYPKHSLLLEATLADGNNQRRMEMEPVSRHLGDYRIRFNNPFD
                     YSLFVSTYLDKNVISDFRYRKIIP"
BASE COUNT          170 a          108 c           74 g          202 t
ORIGIN      
        1 tatggaatta tctttctata tctaaaatct gagattacat ttttatctaa gtatgtgcta
       61 acaaataggc tgtaatcaaa cggattatta aatctaatcc tataatcgcc taaatgtctt
      121 gaaacaggct ccatttccat tcttctttga ttatttccat ctgcaagggt tgcttcaagc
      181 aataatgaat gtttcggata agaggtgcac gcttcatatt catatataat gtctgcgtaa
      241 cctccagctg cgtgggtctt cggcaataaa ttggcttcta atgataactt catgaaatcc
      301 aaaatatttc cttgtctttc gctcactttg taccatataa ttcccaaaat atattcaaaa
      361 atagtaggta ttgcagcctc atctgtaaca agttcttcaa tccttttatc atcacgtttc
      421 tcaaaacaat ttagtaactc tataagcaca gaatcgttaa atttcttatc gatcaaagta
      481 ttaaatcttc tgtatcgttc atcatttaca taagttgcag cttgttccgg actcttaatt
      541 gtaattccta aatc
//
LOCUS       DANLXQ010000331          546 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.339, whole genome shotgun sequence.
ACCESSION   DANLXQ010000331 DANLXQ010000000
VERSION     DANLXQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.339"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(106..>546)
                     /locus_tag="PXD94_002737"
     CDS             complement(106..>546)
                     /locus_tag="PXD94_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL6287530.1"
                     /translation="ATGSRVSELIQIKCEHIKIGYLDLYSKGGKLRRIYIPKSLQKEC
                     SEWLRLNNKHNGFIFLNKYGDRITTRGIASQLKVFARRYKLDPKIVYPHSFRHRFAKS
                     FLERFNDIAFLADLMGHESIETTRIYLRKTSTEQQEIVNKIIDW"
BASE COUNT          184 a           91 c           79 g          192 t
ORIGIN      
        1 gaacaacata gaatcgttga aaaaattgaa aaattatttt cggaaataga gaaattttag
       61 aaaaacttat atgtccaagg gcgtgtgaaa cacgcccctt aaaaattacc aatcaataat
      121 tttattcaca atttcctgct gttctgtgct tgtttttcgt aaatagatcc ttgtggtttc
      181 tatgctttcg tgccccatta aatcagcaag aaaagcaata tcattaaatc gttctaaaaa
      241 actttttgca aaacgatggc gaaatgaatg tggataaaca attttaggat ctaatttata
      301 gcgtctagca aatactttta attggctggc aatgccacga gtagtaattc tatcaccata
      361 tttatttaag aaaataaatc cattatgctt attatttaat ctcaaccatt cagaacactc
      421 tttttgcaga gattttggga tgtaaattcg tcttaatttt ccacctttag aatagaggtc
      481 taaatagcct attttaatat gttcacattt tatctggatt aattcactta ctctcgaacc
      541 tgttgc
//
LOCUS       DANLXQ010000332          539 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.340, whole genome shotgun sequence.
ACCESSION   DANLXQ010000332 DANLXQ010000000
VERSION     DANLXQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.340"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            16..276
                     /locus_tag="PXD94_002738"
     CDS             16..276
                     /locus_tag="PXD94_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDL6287531.1"
                     /translation="MKKLVLSILFVISPMALAYSCNDPIPYCKEMQSCEQAKFYLNQC
                     NAGRLDRDNDGIPCENVCGKKGKSEKKDDKKSDKSKSKKTSN"
     gene            308..>539
                     /locus_tag="PXD94_002739"
     CDS             308..>539
                     /locus_tag="PXD94_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL6287532.1"
                     /translation="MMNNQSVWHLFCDESGISGKPFYAFGALWIREDKISLFEKEIQK
                     LREKHYCTDEIKWQGTNSKRFSEFYRELVSFFF"
BASE COUNT          192 a           76 c          109 g          162 t
ORIGIN      
        1 ataatggggt tcataatgaa aaaattagtc ctctcgattt tatttgttat ttcgcctatg
       61 gctttggctt atagctgcaa tgaccctatc ccttactgca aagaaatgca atcttgcgag
      121 caagcaaagt tttatttaaa tcaatgcaat gccggtcgat tagatagaga taacgatggc
      181 ataccttgtg aaaatgtttg tggcaagaaa ggaaagtccg agaaaaaaga cgataagaaa
      241 tcagataaat caaaatctaa aaaaacttca aattagagac aggcgttgtg cctgattaaa
      301 aggggtaatg atgaataacc agtcagtttg gcacttattt tgcgatgaat ccggtataag
      361 cggtaagcct ttttatgctt tcggtgcatt atggataaga gaagataaaa ttagcttatt
      421 tgaaaaagaa atacagaaat tacgagaaaa gcactattgc actgatgaaa taaaatggca
      481 aggtacaaat tctaaacgct ttagtgaatt ttatcgtgaa ttggttagtt ttttctttc
//
LOCUS       DANLXQ010000333          538 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.341, whole genome shotgun sequence.
ACCESSION   DANLXQ010000333 DANLXQ010000000
VERSION     DANLXQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.341"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(76..>538)
                     /locus_tag="PXD94_002740"
     CDS             complement(76..>538)
                     /locus_tag="PXD94_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287533.1"
                     /translation="GDRNYQLYIDLATGELVSKLGKGLTTREFDAAVKAFENAFNAKD
                     TAENTSPAESKETEQENKQETTKLVITKPTLKGSEKQIAWADEIINLFIAKIGDTSIP
                     VTATQEQITQAQAMVDNFFAMTNAGKWIEKFKGLNDSSEPKQFWLSVKYGW"
BASE COUNT          120 a          110 c           93 g          215 t
ORIGIN      
        1 agtcataata atctccagtt atctgtttgt tttagccgta agcctttgct cttacggctt
       61 atttgttatc tcaatctacc atccatattt tacggataac caaaattgct ttggttcgct
      121 actatcattt agacctttaa atttttcgat ccactttcca gcgtttgtca ttgcaaaaaa
      181 attatctacc attgcttgag cttgagtaat ttgttcttgc gttgctgtta ctgggataga
      241 ggtatcacca atcttcgcaa taaaaagatt gatgatttcg tctgcccaag cgatttgttt
      301 ttcagagcct tttaatgttg gttttgtgat tacaagctta gttgtttctt gtttgttctc
      361 ttgctctgtt tctttgcttt ctgctggcga tgtattttca gcggtgtctt tagcattaaa
      421 agcgttctca aatgctttta cggcagcgtc gaactctctt gttgttaatc ctttgccaag
      481 tttgctcact aactcacctg tggctaagtc aatgtacaac tgataattcc gatcacct
//
LOCUS       DANLXQ010000334          532 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.342, whole genome shotgun sequence.
ACCESSION   DANLXQ010000334 DANLXQ010000000
VERSION     DANLXQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.342"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..500
                     /locus_tag="PXD94_002741"
     CDS             <1..500
                     /locus_tag="PXD94_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287534.1"
                     /translation="IISASIEFSNYFANKYSENIEEGIKNAEKEFKKNYNKSLRYNAK
                     KAQQRVQKYVEKVNQKNPQALKDLDNISNLKKINENLKKAAKFTGIISSIKDYYDLAE
                     GFRNGIKSNDWEPFLKTSAQIGASALMSMLVASMITGPTTIGSIIISAAFISIGGYII
                     SLASR"
BASE COUNT          210 a           74 c           92 g          156 t
ORIGIN      
        1 ggattatatc agccagtatt gaattcagta actattttgc taataagtac tctgaaaaca
       61 ttgaagaagg cattaaaaat gcagaaaaag aatttaaaaa gaattataat aaaagtctaa
      121 gatataatgc taaaaaagca cagcagaggg tgcaaaaata tgtcgaaaaa gtcaaccaaa
      181 agaatcctca ggcactaaaa gatctcgata acatatccaa cttaaaaaaa attaatgaga
      241 atttaaaaaa agcagcaaaa tttactggta ttattagcag tattaaggat tattacgatc
      301 tagcagaagg gtttagaaat ggcataaagt ctaatgattg ggaacctttt ttaaagacat
      361 ctgctcagat aggtgcttca gctttaatga gtatgttagt agcctcaatg ataacaggcc
      421 caacgacaat tgggagtatt attattagtg ctgcatttat ttctataggg ggatatatta
      481 tttcactagc ttcccgttag atagtctttg ttatactcaa taacctacta ta
//
LOCUS       DANLXQ010000335          515 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.343, whole genome shotgun sequence.
ACCESSION   DANLXQ010000335 DANLXQ010000000
VERSION     DANLXQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.343"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
BASE COUNT          185 a           94 c           82 g          154 t
ORIGIN      
        1 aaagattata ctcttttagt acatcaacta attcatcagg taaattttct aacttccatc
       61 ttcgtttttc taaaatcgaa aagtaatact ggtcatcgtc taaaatctca atatatgtac
      121 ttccaaagag aacttttgta taaggcgtat catgatagat cgtgacatca gccttcaatt
      181 tgtactcgcc ttccatgcat tccccaaaat ggtattgact aaaaacaatg gttatgatat
      241 aatgacatgg ggcttgaaaa gcaagctgat tgctcaatag gtaatcagac cactttgaaa
      301 gctcggtcct tttcaaaaga aaaggtctta ctaattactc acaaaatctg gagaacaata
      361 tgaaaaacac tacagcgcaa caaaagccaa tgaaagttac agctaaacat attagagaat
      421 ctactactcg ttgttaagag cttagctttg atataaaaag accaactttt tatatcggcg
      481 tagtaggtct aatttgagcc tacgaaatga aagtg
//
LOCUS       DANLXQ010000336          497 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.344, whole genome shotgun sequence.
ACCESSION   DANLXQ010000336 DANLXQ010000000
VERSION     DANLXQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.344"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>497
                     /locus_tag="PXD94_002742"
     CDS             <1..>497
                     /locus_tag="PXD94_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6287535.1"
                     /translation="QGNLVTAIPNFLEKVNNDFAISARNRDLYSHYLPYQGIDDWENS
                     NFNFRTLNGVYYDDNLTSTELLRKFMIQRDYNTLYALSSARTGNADSLAKNYHLVVAK
                     MSYENLKKELSKKPDSTFNKLKKLYETNGEFLNEYILDNFRGNPLELLTVPALKESLE
                     NQESF"
BASE COUNT          182 a           84 c           79 g          152 t
ORIGIN      
        1 tcaaggtaac ttagtaacag ccattcctaa cttcctggag aaagttaata atgattttgc
       61 tataagtgct agaaatagag acttatatag tcactacctt ccttatcagg gtattgatga
      121 ttgggagaat tctaatttta actttcgaac actaaatgga gtttattatg atgataatct
      181 aacttcaaca gaactcctgc gaaaatttat gattcaaaga gattataaca ctctttatgc
      241 tctaagttct gctcgcacag gaaatgctga tagtctggct aaaaattacc atcttgtagt
      301 tgcgaaaatg tcctatgaaa acctgaaaaa agaattatcc aagaaacccg actctacatt
      361 taataaactg aaaaagctgt atgaaaccaa tggtgaattc ttaaatgaat atattttaga
      421 caatttccga ggaaatccac ttgaattatt aacagtacct gcgcttaaag aaagtttaga
      481 aaaccaagag tcattcc
//
LOCUS       DANLXQ010000337          480 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.345, whole genome shotgun sequence.
ACCESSION   DANLXQ010000337 DANLXQ010000000
VERSION     DANLXQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.345"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>480
                     /locus_tag="PXD94_002743"
     CDS             <1..>480
                     /locus_tag="PXD94_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287536.1"
                     /translation="PIILISAIWSIFTTDILERIANMPIIWKNFEENYLYNKKLDGLW
                     STNTNYIIDYKLLNIPEEQPNVIIEFMVNKDDNSILGEMTSTALCELIPLTWSFRLES
                     PKPTLLNLIFDRELKLSYLVSGGYTELSTFKIKEINSNLGTLTLTTQEDKTGIFPPTI
                     "
BASE COUNT          176 a           75 c           77 g          152 t
ORIGIN      
        1 cctattattc ttatatctgc catatggagt atcttcacaa ctgatatttt agaacgcatc
       61 gcaaacatgc ctattatttg gaaaaatttc gaggagaatt acctatataa taaaaaactg
      121 gatggtttat ggtctacaaa tacaaattac attatcgact ataaattatt gaacatacct
      181 gaagagcaac caaatgttat cattgaattt atggttaata aagatgacaa ttcgatttta
      241 ggggaaatga cttcaacagc tctgtgtgag ttaatacctc taacttggag ttttagattg
      301 gaaagcccta agccaacatt attaaatctt attttcgata gagagttgaa attgagttat
      361 cttgttagtg ggggatatac cgagctatca acatttaaaa ttaaagaaat taacagtaat
      421 ctaggaacac taacattaac tacgcaagaa gataaaaccg gtatatttcc tccgacaatc
//
LOCUS       DANLXQ010000338          472 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.346, whole genome shotgun sequence.
ACCESSION   DANLXQ010000338 DANLXQ010000000
VERSION     DANLXQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.346"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            95..>472
                     /locus_tag="PXD94_002744"
     CDS             95..>472
                     /locus_tag="PXD94_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL6287537.1"
                     /translation="MNLIICCTPLQVLIAEKIIEMHPNERFYGVMLSTVKNAKFDFYQ
                     ARLAQKCEQFFAMQQHSERIQLLKEIIYLKRTFGGKKFNKVFLANINELEIQFLLSAI
                     SFQELNTFDDGTANIVQNSIFYQA"
BASE COUNT          161 a           75 c           79 g          157 t
ORIGIN      
        1 ctaaaattga atatattcct tttgccagca tcattggtgc aatcagcatt attccgattc
       61 tttatataat gacggcaaaa gtaggaaaaa ccgtatgaat ttaattattt gttgcacgcc
      121 cttgcaagtg ttgattgctg aaaaaatcat tgaaatgcac cctaatgagc gattctatgg
      181 ggtgatgctt tctacggtaa aaaacgcgaa atttgatttt taccaagcta gattagccca
      241 aaagtgcgaa caattttttg cgatgcaaca acatagcgag cgtattcaat tattaaaaga
      301 aattatttat ttaaaacgta cttttggcgg taagaagttt aataaagtct ttttagcaaa
      361 tatcaatgag ttagaaattc aatttctgtt aagtgccatt tcatttcaag aactcaatac
      421 ctttgatgat ggcacagcta atattgtaca aaacagtatt ttctatcaag ct
//
LOCUS       DANLXQ010000339          467 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.347, whole genome shotgun sequence.
ACCESSION   DANLXQ010000339 DANLXQ010000000
VERSION     DANLXQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.347"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            34..>467
                     /locus_tag="PXD94_002745"
     CDS             34..>467
                     /locus_tag="PXD94_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL6287538.1"
                     /translation="MPNFSFTHLYKKHTKELPTVTLLSALIGLSQVSYANPLPLGNTT
                     GTNNLAVGEGSFALGTGSQAIGKNAIATGGNISPQEFNVELENFRDLVNQIEELRTQI
                     AEQEKQGSVNQQLQDALKSQIAQYDTILERVREKQAQQAILA"
BASE COUNT          159 a           96 c           87 g          125 t
ORIGIN      
        1 gaaaagtaaa agttttaatg aggaaatttt gatatgccaa actttagttt cacccatctg
       61 tataaaaaac acacaaaaga gctccctact gttaccttgc ttagcgctct gatagggctt
      121 agccaagtct cttatgccaa tccacttcct ttaggcaaca ccactggcac caataacctt
      181 gctgtaggtg aaggtagttt tgcgttaggc acaggttctc aagcaatcgg caagaatgcc
      241 attgcaaccg gtggtaatat ctccccccag gaattcaatg tggagttaga aaacttccgt
      301 gatttagtta atcaaattga agagttacgt actcaaattg ctgaacaaga aaaacaaggc
      361 tctgttaatc aacaattaca agatgcttta aagagtcaga ttgctcaata tgacactatt
      421 ttagagcgtg tacgtgaaaa acaagcacaa caagcaatat tagccaa
//
LOCUS       DANLXQ010000340          449 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.348, whole genome shotgun sequence.
ACCESSION   DANLXQ010000340 DANLXQ010000000
VERSION     DANLXQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.348"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>449)
                     /locus_tag="PXD94_002746"
     CDS             complement(<1..>449)
                     /locus_tag="PXD94_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha2 family transposase"
                     /protein_id="HDL6287539.1"
                     /translation="QHKLIELFVAGVTARTAAELVNVNKTTAAYYFHRLRLLIYQHSP
                     HLEMFEGEIEADESYFGGARKGKRGCGAAGKTAVFGLLKRDDKVYTVVVPNTQSATLL
                     PIIREKVKPDSLVYADFYRSYDVLDVSEFNHFRINHSTHFAENHNHI"
BASE COUNT          125 a          102 c           95 g          127 t
ORIGIN      
        1 ttaatgtgat tgtgattttc agcgaagtgt gtgctgtgat tgatacgaaa atggttaaat
       61 tcactcacat caagcacatc atagcttcga taaaaatcgg cgtaaacaag gctgtctggc
      121 ttcactttct cccgaataat cggcaaaagt gtggcagatt gcgtatttgg tacgacaacg
      181 gtataaacct tgtcatcacg ttttaacagc ccaaatacgg cagttttgcc tgcggcacca
      241 caccctcgtt tacctttgcg agcaccaccg aaataacttt cgtccgcttc aatttcaccc
      301 tcaaacattt ccagatgagg gctatgttga tagatgagca atcgcaaacg gtgaaagtaa
      361 taggcagcgg tggttttgtt tacattcacc aactcagccg ctgttcgagc agtgacacct
      421 gctacaaaca gttcaatgag tttatgttg
//
LOCUS       DANLXQ010000341          443 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.349, whole genome shotgun sequence.
ACCESSION   DANLXQ010000341 DANLXQ010000000
VERSION     DANLXQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.349"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(75..>443)
                     /locus_tag="PXD94_002747"
     CDS             complement(75..>443)
                     /locus_tag="PXD94_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287540.1"
                     /translation="EFGELCGGIARNNPEMIKDAIGDLIVVCIIKAKQEKQPIYPVQF
                     ENHRLNGYRFDISVAVHCLYDIERIKDRGKYSRFFGILHHLALINRFTLNDCLAHAYD
                     QIKDRKGEMQAGIWVKEDDL"
BASE COUNT          133 a           86 c           79 g          145 t
ORIGIN      
        1 aagaagaaac cgaaactctt atagagtggc ggaatgcttt tctgcaagat gtttcggttt
       61 ttatagctta ggtactataa atcgtcttct ttaacccaaa tcccagcttg catttcgccc
      121 ttgcgatctt tgatttggtc gtaggcgtga gcgaggcagt cattaagcgt aaatctattt
      181 attaaggcta aatggtgtaa tatgccaaaa aaacggctat atttacctct gtctttaatg
      241 cgttcaatat catatagaca atgaacagcg accgaaatat caaatctgta accatttaat
      301 ctatgatttt caaattgtac agggtaaata ggttgttttt cttgttttgc tttaataata
      361 cagacaacaa tcaaatcccc aatcgcatct ttaatcattt caggattatt gcgggcaatg
      421 cctccgcaga gttcgccaaa ctc
//
LOCUS       DANLXQ010000342          431 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.350, whole genome shotgun sequence.
ACCESSION   DANLXQ010000342 DANLXQ010000000
VERSION     DANLXQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.350"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>431
                     /locus_tag="PXD94_002748"
     CDS             <1..>431
                     /locus_tag="PXD94_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL6287541.1"
                     /translation="IFISAGGVRSIWLRHNLANFKQRLNALEKAVAEKGIILSENQVQ
                     ALERKKEDDVACGEIETAHPGYLGSQDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLY
                     TMKTAISAADMLNDKVLPFFESQGLPMLRILTDRGSEYCGKV"
BASE COUNT          126 a           78 c          105 g          122 t
ORIGIN      
        1 atttttattt cagcaggtgg tgtgcgttct atttggctac gccataatct cgctaacttt
       61 aaacagcgtt taaatgcgtt agaaaaagca gtggctgaga aaggcattat tctgagtgaa
      121 aaccaagttc aagccttgga gcgtaaaaaa gaagacgatg tggcttgtgg cgaaattgaa
      181 acggctcatc cgggctatct aggttcacaa gataccttct atgtggggaa tcttaagggg
      241 gttggacgca tttatcaaca gacctttatt gatacttata gtaaagtcgc atttgcaaaa
      301 ctgtatacga tgaagacagc aattagtgca gcagatatgc tcaatgataa agtcctaccg
      361 ttctttgagt cccaaggatt accgatgttg cgtattctta ccgaccgagg cagtgagtac
      421 tgtggcaagg t
//
LOCUS       DANLXQ010000343          429 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.351, whole genome shotgun sequence.
ACCESSION   DANLXQ010000343 DANLXQ010000000
VERSION     DANLXQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.351"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            10..249
                     /locus_tag="PXD94_002749"
                     /pseudo
     CDS             10..249
                     /locus_tag="PXD94_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
BASE COUNT          150 a           62 c           95 g          122 t
ORIGIN      
        1 gagccttata tgaaattcaa aaaattagca ttagcagcaa tgttaggtgg cttatttgta
       61 acagctaatg cggcagaatt acctaatatc actattttag cgacaggcgg aacgattgct
      121 ggtagtcagc agaatgcaaa tagtagtgct tatcaagcag ggcaattgaa tatcgaaacc
      181 ttaattgaag cagtgccaga aatgaaaaca ttggcgaata tcaaaggtga gcagattgtg
      241 aaaattggcc cactaaagcc ttgttagatg taggctatca aggtattgtc gtagcaggtg
      301 ttggtaacgg caatatgagt gcggaaaatc ttgcattatt tagaggaagc caacaatatt
      361 tttaagattt ttaataaacg ttaaagcata taaaaaatgg cataagtgag tcacttatgc
      421 cattatttt
//
LOCUS       DANLXQ010000344          428 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.352, whole genome shotgun sequence.
ACCESSION   DANLXQ010000344 DANLXQ010000000
VERSION     DANLXQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.352"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>428
                     /locus_tag="PXD94_002750"
     CDS             <1..>428
                     /locus_tag="PXD94_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL6287542.1"
                     /translation="FVSAGGVRSIWLRHHLANFKQRLIALEKLVAEQGIILSETQVQA
                     LERKKEDEIACGEIETVHPGYLGSQDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLYT
                     MKTAISAADMLNDKVLPYFESQGLPMLRILTDRGSEYCGKV"
BASE COUNT          133 a           74 c          100 g          121 t
ORIGIN      
        1 tttgtttcag caggaggtgt acgttcgatt tggttacgtc atcatcttgc caattttaag
       61 caaagattaa tcgccctgga aaaactggtt gcagaacaag gtattatact cagtgaaaca
      121 caggtacaag ccttagagcg taagaaagaa gatgagattg cctgtggtga aattgaaacg
      181 gtacaccctg gctatcttgg ctcacaagat actttctatg tgggcaacct caaaggagtg
      241 ggtcgtattt atcaacaaac gtttattgat acttacagta aagtggcgtt tgcaaaactt
      301 tatacaatga agaccgctat cagtgctgcg gatatgctga atgataaagt gttaccatac
      361 tttgaaagcc aaggtttacc gatgttacgc atattaactg accgaggaag tgaatattgt
      421 ggcaaggt
//
LOCUS       DANLXQ010000345          418 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.353, whole genome shotgun sequence.
ACCESSION   DANLXQ010000345 DANLXQ010000000
VERSION     DANLXQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.353"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            140..>418
                     /locus_tag="PXD94_002751"
     CDS             140..>418
                     /locus_tag="PXD94_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDL6287543.1"
                     /translation="MKNRIEHPKIFISYAWGSQEHDEKVIALATNLKGDGIDVIFDKW
                     QLKEGNDTFKFMEKSVLDESITNVLILIDPIYAKKANERAGGVGTETQI"
BASE COUNT          158 a           45 c           96 g          119 t
ORIGIN      
        1 taccatgaaa tgccattaga gactgtagat tggcatgatg ggatgataaa agaggctact
       61 ggagagtata agtcatagtg attataagat agtgagaaag aatacttatt ttgaaaggaa
      121 tacatgggaa ttgatacaga tgaagaatag aattgaacat cctaagattt ttatttcata
      181 tgcttgggga tcacaggaac atgatgagaa ggttattgct ttagctacaa atcttaaagg
      241 agatggtata gatgtcattt ttgataaatg gcagctaaaa gaaggcaatg atacctttaa
      301 attcatggaa aaaagtgttt tagatgagag tattaccaat gtactgattc ttatagatcc
      361 tatttatgca aagaaagcaa atgaaagagc cggtggcgta ggtacagaaa cacagatt
//
LOCUS       DANLXQ010000346          388 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.354, whole genome shotgun sequence.
ACCESSION   DANLXQ010000346 DANLXQ010000000
VERSION     DANLXQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.354"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..>388)
                     /locus_tag="PXD94_002752"
     CDS             complement(<1..>388)
                     /locus_tag="PXD94_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL6287544.1"
                     /translation="SSGEYIARMDADDISLPTRLSEQILYLEENHLDLCGCNALLFSE
                     HGNLGLTNKLNSHEALEKLMVAGDIGIIHPTFLARRKVFVESGGYNPLALHAEDMEFI
                     AHILSMGFRLGNNPKVLFKYRFSTNSV"
BASE COUNT          138 a           79 c           51 g          120 t
ORIGIN      
        1 taacgctatt tgtagaaaac ctatatttga ataatacctt aggattattc cctaatctaa
       61 atcccattga taaaatatgg gcaataaatt ccatatcttc cgcatgtaaa gctaagggat
      121 tataccctcc agattcaaca aatacttttc tccttgctaa aaaagtaggg tgaattattc
      181 caatatcacc agccaccata agcttttcta aagcctcatg actattcaac ttatttgtaa
      241 gaccaaggtt gccatgttca gaaaaaagca aagcattaca accgcataag tctaaatggt
      301 tttcttctaa atataaaatt tgctctgaca atctagtagg taaagatata tcatcagcat
      361 ccattctcgc tatatattca ccagagga
//
LOCUS       DANLXQ010000347          370 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.355, whole genome shotgun sequence.
ACCESSION   DANLXQ010000347 DANLXQ010000000
VERSION     DANLXQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.355"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            43..>370
                     /locus_tag="PXD94_002753"
     CDS             43..>370
                     /locus_tag="PXD94_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL6287545.1"
                     /translation="MSYKVVFSLLFSLFLSTANANWTKINDVNYTWGPFNIYHITLFT
                     ESGTYVEGTRPLMLTLQYKKPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTF
                     PDIKPED"
BASE COUNT          125 a           53 c           73 g          119 t
ORIGIN      
        1 ataataatga gtaggaattt tagatttaag gacacaaaaa taatgagcta taaagtggtc
       61 ttttcgctgt tgttttctct atttttatca acagcaaatg caaattggac aaaaattaat
      121 gatgtgaatt atacgtgggg gccatttaat atttaccata ttacgctttt tactgagagc
      181 ggtacttatg tagaaggtac acgtccttta atgcttactc tacaatataa aaaaccgtgg
      241 gaggggcgtg attttgcggt ttctttagca agaacttgga ataatttagg cattaagcta
      301 cctgaaaaag agttagatga ggttattacc cacctgagaa aaactttccc agatattaag
      361 cctgaagata
//
LOCUS       DANLXQ010000348          366 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.356, whole genome shotgun sequence.
ACCESSION   DANLXQ010000348 DANLXQ010000000
VERSION     DANLXQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.356"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>366
                     /locus_tag="PXD94_002754"
     CDS             <1..>366
                     /locus_tag="PXD94_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287546.1"
                     /translation="NTTNVDLKITIGTRQYSHSFNGKYSSQYLESVVFDNLPPVPFNI
                     SVERLTEDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAVAGISFDSEYFNNIPTRNYLI
                     KAKKVKVPSNYDPVKRTYTG"
BASE COUNT          117 a           84 c           67 g           98 t
ORIGIN      
        1 aataccacca atgttgattt aaaaatcacg attggcacac ggcaatattc gcatagcttt
       61 aacggcaaat acagctcgca ataccttgaa tctgtggtgt ttgataacct gccgcctgtg
      121 ccatttaata tctcagttga gcgtctaacc gaggatagca attcgcaacg cttacaaaac
      181 ggtacaattt ggagtagtta caccgaaatt atcgataccg agtttaccta ccctaattca
      241 gcggttgccg gtatcagttt tgattcggag tattttaata atatcccgac ccgaaactac
      301 ctaattaaag cgaaaaaagt caaagtgcca agcaattatg atccagttaa acgcacttac
      361 accggc
//
LOCUS       DANLXQ010000349          362 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.357, whole genome shotgun sequence.
ACCESSION   DANLXQ010000349 DANLXQ010000000
VERSION     DANLXQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.357"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(78..154)
                     /locus_tag="PXD94_002755"
     tRNA            complement(78..154)
                     /locus_tag="PXD94_002755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Arg,seq:acg)
     gene            complement(168..262)
                     /locus_tag="PXD94_002756"
     tRNA            complement(168..262)
                     /locus_tag="PXD94_002756"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Ser,seq:gct)
BASE COUNT          101 a           75 c           80 g          106 t
ORIGIN      
        1 gggtgcgtat atttgaattg taagtttatt taattttatc agcaaccatt ttaatttgaa
       61 tagagtaaaa attaaaatgg cgcacccgag aggattcgaa cctctgaccg ctcggttcgt
      121 agccgagtac tctatccagc tgagctacgg gtgcgtgata aagtaaatgg cggtgagaga
      181 gggattcgaa ccctcgatag agctttttaa ccctatactc ccttagcagg ggagcgcctt
      241 cagcctgctc ggccatctca ccactcactg tgtctgtggg cgccatatta ctagaattta
      301 aaaagatgtc aaattctttt tcctgaaaat tgaaaaatcc cgagtgtttg cctattgaat
      361 aa
//
LOCUS       DANLXQ010000350          361 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.358, whole genome shotgun sequence.
ACCESSION   DANLXQ010000350 DANLXQ010000000
VERSION     DANLXQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.358"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>361
                     /locus_tag="PXD94_002757"
     CDS             <1..>361
                     /locus_tag="PXD94_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287547.1"
                     /translation="SLFNGKVLVLTGQAGSGKSHLLGDFVQKRQNSGKYSLLLLGQDF
                     TEKSNAWNQILSKQLNLNITPEVFLSALDSIGEMQKERVILAIDALNEGEGKSLWNNQ
                     LNGLIELVSQYPNIGLVL"
BASE COUNT          124 a           48 c           72 g          117 t
ORIGIN      
        1 atcattattt aatggtaaag tgcttgtttt aactggtcaa gctggtagtg gaaaatctca
       61 tttattgggt gattttgtcc aaaaaagaca aaattctgga aagtattctt tgttgttatt
      121 ggggcaggat tttacagaaa aatccaatgc ttggaatcaa atcttatcca aacaattaaa
      181 tcttaatatc acacccgaag tatttttatc cgcattagat agtatcggag aaatgcaaaa
      241 agaacgggtt attttagcca tagacgcctt aaatgagggt gaagggaaat cattatggaa
      301 taaccaactt aatggattaa ttgagttggt aagtcaatat ccaaatattg gactggttct
      361 g
//
LOCUS       DANLXQ010000351          354 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.359, whole genome shotgun sequence.
ACCESSION   DANLXQ010000351 DANLXQ010000000
VERSION     DANLXQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.359"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            31..107
                     /locus_tag="PXD94_002758"
     tRNA            31..107
                     /locus_tag="PXD94_002758"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65..67,aa:Ile,seq:gat)
     gene            164..239
                     /locus_tag="PXD94_002759"
     tRNA            164..239
                     /locus_tag="PXD94_002759"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ala,seq:tgc)
BASE COUNT          107 a           75 c           90 g           82 t
ORIGIN      
        1 aagaagacaa aaaaccgaag gtcatccgtt gggtctgtag ctcaggtggt tagagcgcac
       61 ccctgataag ggtgaggtcg gtggttcaag tccactcaga cccaccactc agaaggcaag
      121 cgaaagcgaa gcgactatga gtgaagaaaa cggaagatga aatggggata tagctcagct
      181 gggagagcgc ctgccttgca cgcaggaggt cagcggttcg atcccgctta tctccaccaa
      241 tcatcgtgac tttatcatca tacctaagat tacaagcggt taaaaatacc ggattatttg
      301 caattttagg tatgatgata ggcgaaagcc attatctttg ttctttaaca atat
//
LOCUS       DANLXQ010000352          349 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.360, whole genome shotgun sequence.
ACCESSION   DANLXQ010000352 DANLXQ010000000
VERSION     DANLXQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.360"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>349
                     /locus_tag="PXD94_002760"
     CDS             <1..>349
                     /locus_tag="PXD94_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL6287548.1"
                     /translation="AGQERCIADEVPFEIPQNWIWVRLENYSLNHDRRRKPVSVAQRS
                     QQNKLYDYYGATGAIDKVASYIFDGKFILIGEDGGNFFTKKDVAFIVEGKFWANNHVH
                     VLSVDFNLEKYFCY"
BASE COUNT          116 a           37 c           78 g          118 t
ORIGIN      
        1 gcaggtcagg aacggtgtat cgccgatgaa gtgccatttg agattcctca aaattggatt
       61 tgggtgaggt tggaaaatta ttctttaaat catgatagaa gaagaaaacc agtatcagtt
      121 gcacagcgta gccaacaaaa taaattgtat gactattatg gagcgactgg agcaatagat
      181 aaagtggcaa gctatatttt tgatggtaag tttattttaa ttggagaaga tggaggtaat
      241 ttcttcacta aaaaagacgt tgcttttatt gttgaaggta aattttgggc aaataatcat
      301 gttcatgtat tatcagttga ttttaatttg gaaaaatatt tttgttatt
//
LOCUS       DANLXQ010000353          338 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.361, whole genome shotgun sequence.
ACCESSION   DANLXQ010000353 DANLXQ010000000
VERSION     DANLXQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.361"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..>338
                     /locus_tag="PXD94_002761"
     CDS             <1..>338
                     /locus_tag="PXD94_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL6287549.1"
                     /translation="AVDSLSIGARNLLIDSTYNQHINYNTKMSSITRTVYRGNMLIRL
                     GMLGGAGVAGIVQAPAAQVSNIRQGQEYTLSLNVQGTAGIQKTGLNYVFLMRADGANQ
                     RLATIPVIAS"
BASE COUNT          104 a           79 c           76 g           79 t
ORIGIN      
        1 ctgccgttga ttcactttct atcggtgcaa ggaacttact gatagacagt acttacaacc
       61 aacacatcaa ctacaataca aaaatgtcat caatcacacg cacagtctat cgcggcaata
      121 tgttgatacg actcggaatg cttggtggcg caggtgtggc cggtattgta caagcaccag
      181 ccgctcaagt aagtaatatc agacagggtc aagagtacac acttagcctg aatgtacagg
      241 gtacagccgg catacaaaaa acggggctca actatgtatt cttgatgcgt gctgatgggg
      301 ctaatcaacg gttagcaaca atacctgtta tagccagc
//
LOCUS       DANLXQ010000354          324 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.denovo.362, whole genome shotgun sequence.
ACCESSION   DANLXQ010000354 DANLXQ010000000
VERSION     DANLXQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.denovo.362"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..226)
                     /locus_tag="PXD94_002762"
     CDS             complement(<1..226)
                     /locus_tag="PXD94_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HDL6287550.1"
                     /translation="MFFYRHYLRADTKRSYNNIIRIIKKRYPNLNANDVTTDMLIEWR
                     NQVVGNTIKAVTWNNYVRHLKALYNFAIAQG"
BASE COUNT          104 a           62 c           44 g          114 t
ORIGIN      
        1 gcccctgtgc tatcgcaaaa ttatataatg cttttaggtg tctcacataa ttattccacg
       61 tcacagcttt aattgtatta ccaacaactt gattacgcca ttcaattaac atatcagtag
      121 tcacatcatt cgcatttaag tttggataac gctttttgat aattcgaata atattgttat
      181 aacttcgttt cgtgtccgcc cgtaaataat gacgatagaa aaaaatcgtt ccaataatat
      241 atcgaaggaa atcttagaat ccatattatg tgtcctcttc ctgtaaagtt aaacagtggt
      301 tatttagtaa cattttatcc ccaa
//
LOCUS       DANLXQ010000355         5028 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.guided.168, whole genome shotgun sequence.
ACCESSION   DANLXQ010000355 DANLXQ010000000
VERSION     DANLXQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.guided.168"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..642
                     /locus_tag="PXD94_002763"
     CDS             <1..642
                     /locus_tag="PXD94_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL6287551.1"
                     /translation="AKEKAEAERLAAEKEVDPIAIIKAKGIDDTNYGLKLGNSKETIE
                     GKVTVRTQSYLYNQPYSVVTAQAVQRNGWENGNYIDKVDGTVTVKGLKTEKLPTEGTA
                     TYSGKAFNHDGAKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLIVLEEGTINNTQISST
                     VHGNNPLNLSGKYTVEFFGKNAEEIAGEIQTKANLTGEVSNFGLAGTRGDIQK"
     gene            838..2247
                     /locus_tag="PXD94_002764"
     CDS             838..2247
                     /locus_tag="PXD94_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL6287552.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            2393..2866
                     /gene="gpt"
                     /locus_tag="PXD94_002765"
     CDS             2393..2866
                     /gene="gpt"
                     /locus_tag="PXD94_002765"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL6287553.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            2979..3602
                     /gene="gmk"
                     /locus_tag="PXD94_002766"
     CDS             2979..3602
                     /gene="gmk"
                     /locus_tag="PXD94_002766"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL6287554.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            3692..>5028
                     /locus_tag="PXD94_002767"
     CDS             3692..>5028
                     /locus_tag="PXD94_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL6287555.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAK"
BASE COUNT         1531 a          959 c         1125 g         1413 t
ORIGIN      
        1 gcgaaagaga aagcggaagc ggagcgttta gcagcagaaa aagaagtaga tcctatagct
       61 attattaaag caaaaggtat tgatgataca aattacggct taaaacttgg taatagtaaa
      121 gaaactattg agggtaaagt cacagtcaga acacagagtt atttatataa tcagccatat
      181 tctgtagtaa cagcacaagc agttcaaaga aatggctggg aaaacggtaa ttatattgat
      241 aaagttgatg gaacagttac agttaaagga ttgaaaacag agaaattacc aacagagggt
      301 actgcaacat attcaggaaa agcatttaac cacgatggag ctaaaggttt taatggtggt
      361 gatctaacct ataatgttaa tttctctaag cgtaccggtt caggtagagt tgagggagaa
      421 tttggaaact taatcgtatt agaagagggg acgattaata atactcagat ttcttcaaca
      481 gtacatggca ataatccttt aaacctttct ggaaaatata cagtcgaatt tttcggtaaa
      541 aatgcagagg aaattgccgg tgaaattcag acaaaagcaa atcttactgg ggaagtgagt
      601 aattttggac ttgccggcac tcgtggcgat atccaaaaat aaccttttct aacgttcaat
      661 gccgtttaac gaaaaattaa acggcatttg tcttgatatt gcttgtagat accataagac
      721 acaatagttt agatttcaat ttacagcttc taataaccct ctcccacaag gggagagagt
      781 gataaatccc gtagaggcga acatataggt ccgcccctac caattttctt tatctttatg
      841 aaaaaatatc cccttttatt ttcgctgttc agccccgttg tatgggcaga aaaggtgatt
      901 gaacacaaca ctgacccgat ttcctttgag cgtcagccgc aagcggttaa atttgagcaa
      961 aatgttgtaa aaaattcaca aaaaccgacc gcttatcagc ctgcgtattc agtcaatcag
     1021 aatgttgaac agcaaattaa ccaagcgatt ctcagccaag attgggcaac gcttgaaccg
     1081 cttttaagcc gttatccgca aactgaaaat tttgacccgt tgttgtggca ctatgcgcaa
     1141 gccgcacttg cctatgcgaa aaaagatcat caaaccgcag tttttcacta tcgccaactg
     1201 ttagctaagc ccccgagttt ggtttaccct cgttttgatt tggcgttgat tttggcagaa
     1261 gatcagcaat ttcgtgcggc agagcaggaa tttaaggcta ttcaagccca tttaccgccg
     1321 gaacttgcta aaattgcccg caatactcaa gtccaaattg ctgaaaatga ggcgtggcag
     1381 cccgattttt atctgcaata tacccaaaca gataacgtga ataatgcctc atcttcgccg
     1441 attgtgaatg taaatgggcg gattttccgc aaaaatgcag acagtttgcc acaatcagca
     1501 aaaggcgtgc gttatggctt agggttgagt aaattgcaca acgtgaaagg caatcatttt
     1561 gtcggtttaa cgctcaatta tagcgggatt tactattggg ataaccgaga ttaccgtgag
     1621 caatccgtat ccgtttcgcc gaattatagc taccgcaccg tcgcttatcg gctgactttt
     1681 tcgcctttta ttgagcaaaa ttggctgggg agtagccgtt ataatgttca atttggggca
     1741 acactcaacg gtttgcgtta ccttaattcg caatggttgg tgtcggggag ttttacccat
     1801 ttgcaaaaac ggtattttga ggagctaacc gcaagccgtt ataacagcta tcagcaccaa
     1861 ctcagtggcg gtgtgcagtg gcaagcggtc aaaaatgggc ggttttttgc aaatttagac
     1921 ggcgtgcgtg aaattgcacg ggaaaaagca caaagctcga ataagtggct ggctcgagtg
     1981 ggggcggttt ttcaaggcga acgctgggca acgcaggcaa gtatcggctt tggcaagcga
     2041 tattttgccg acaagcacta tttgtttggc tataagcggt tagacacgga atatcaagcc
     2101 aatttagcgg tttggaaccg cacttggcat tggcagggca ttgtgccgaa gctgaatttc
     2161 cgttatcaaa aaattgacag caatattgct gatttttact ctcgccaaaa caaggaagtc
     2221 tttttcactt tagaaaagct attttagtgt tggttttttg tgctaagcat acgattgcgt
     2281 agctgccggc acaaaaaaat aaatgaaatg atggcgaaaa aagcattttc gggttatcct
     2341 agtgcaattt tttatatcgg ggtagccccc aattttatat taggttgttt ctatgagtga
     2401 aaaatacaca aatgaaaaat atgtcgtgac gtgggatatg ttccatatgc acgctcgcaa
     2461 gttggcagaa aatttattac ctgccacaca gtggaaaggg attattgcgg tgagccgtgg
     2521 cggcttgttt ccggcagccg ttttagcacg tgagttgggt attcgccacg tagaaacagt
     2581 gtgcattgcc agctacgatc acgatagcca aggtgagtta aatgtgttac acgcagccga
     2641 taccgatggc gaaggcttta ttgtggtaga tgatttagtg gataccggca acacggcaaa
     2701 agaaattcgc agaatgtacc cgaaagctcg ttttgtaacc gtgtttgcga aaccgcacgg
     2761 tgcaccgttg gttgatgatt atgtgattga tattccacaa gaaacgtgga ttgagcagcc
     2821 ttgggattta ggcattacct tcgttcagcc tttggcacgt agataatttt gtgtaatgga
     2881 caatgaaagt tgtccattat tttttattta ttcctatgaa atgtaggggc gaatcatcat
     2941 tcgccctatt cccaaaatca aataggtata tacaaataat gagtttaggc aatctttata
     3001 ttatttctgc cccaagtgga gcaggaaaat cttccttaat taacgccttg ttggcggatt
     3061 taccccgttc ggaagtacag ctttcgattt cacacactac ccgtcagcct cgtccggcag
     3121 aacaggacgg cgtgcattat tatttcactt cgcacgatga gtttaagcag ctgattgagc
     3181 aagggtattt tttagaatgg gcggaggtgt ttggcaatta ttacggcaca tcattgccga
     3241 tgattgaacg tagcctagag cagggcattg atgtcttttt ggatattgac tggcaagggg
     3301 ctagacaaat tcgggaaaaa gtgccgaatg tgaaaaccgt ttttattttg ccaccgtcaa
     3361 aaacagaatt ggaaaaacgt ttaatcgggc gtgggcaaga ttccgctgaa accattgcca
     3421 aacgtatggc agaggcggtg tcggaaatga accattacaa tgagttcgat tatgtgatta
     3481 ttaatgatga tttccaacag gcattggcag agctaaaagc gattttaatt gccgagcgtt
     3541 tgagcttagg ttcgcaacaa attcgtcatc agcatttgat tactgatttg ttaaataatt
     3601 aaatgaacag ttgttcattt aactggaaag gcaatctgtt tttgggtaga atacgcccat
     3661 tttcccctct ttattttcaa aaggaaatgt aatgaaactt tcaaaattag cattttctgt
     3721 ggcgttggct ctaaccgtaa cggcttgtac cacagggaat atgagcaatg acgggacttt
     3781 gcaacaagca cagcaaacct atcaaagcta tgatgaaatt accaaacaat atcaaattaa
     3841 cgagcaatgg tggcaaggct atacggatgc ggaattaaac cgtgtggttg atttagccat
     3901 caaaaataac cttgatttag ccaaaagcgg aattgcagtc aataaagcct tgtataacgc
     3961 taatttagta ggggcaaatt tagtgccaac ctttagtggt tcaggttctg tttctgcggc
     4021 taaaggttta ggttcggctt cttcaaataa tgtttcgacc ggtgtttcaa caaccgcagc
     4081 tcaagctgca tttaatttaa gttatactgt ggatttatgg cgacgtttag ccgataccgc
     4141 cagtgcggca gaatggacgc ataaagccac cattgaagac ttaaaagcta cccgtttatc
     4201 tattattaat gcggttgtga ctacttacta ccagttagct tactatcgtg atgcgattcg
     4261 tattaccgaa gagagcattc gccattatga gcaaatcagc cgtattttaa ctaacaaaat
     4321 gaatgccggt gcgattgata gtttagcggt tgatcaggct caacaagcgg tcttaaacgc
     4381 cagaaatagt gtaattgatc tgaaaaccaa ccaaaaaatt gccgagcaaa cattacgcaa
     4441 tttgttaaat gttcagccaa atactccgct taacattcgt tacccgaata ttatgagcgt
     4501 gaaattgcaa ggggtagatt taaatgtgcc agtgtcagcc attgctaatc gccctgatgt
     4561 agtaggctcg ttacaacgct tacaaagtgc atttaaaacc ttaaccgcaa ccgagaaaag
     4621 ctggttccca accttaacca tcggtggttc tattaccggt agtgcaacca atatcggcaa
     4681 tgtggcagat aaccctgtgg gtaacggctt aattaaattg gatttaccgt tcttagattg
     4741 gaatcgggtt aaaaataacg taaaaatttc tgaaacggat tatgtgactg cccgtttaaa
     4801 ctatgagcaa aaaattacct cagccttaaa tgaaattgac cgttattatt acagttatac
     4861 gcagtcgaga agctcgttaa aaatccttca agataccaat gcgaaaaata aacgtatcag
     4921 ttcatactat aaaaaccgtt atgaacaagg ggtgtcggag ttccgtgagt ggattaccgc
     4981 actgaatacc gagcttaatt ctcagttggc gattttaaat gcaaaata
//
LOCUS       DANLXQ010000356         4818 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.guided.174, whole genome shotgun sequence.
ACCESSION   DANLXQ010000356 DANLXQ010000000
VERSION     DANLXQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.guided.174"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            complement(<1..218)
                     /locus_tag="PXD94_002768"
     CDS             complement(<1..218)
                     /locus_tag="PXD94_002768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287556.1"
                     /translation="MPENSDWISCSGCENSRSSGKKPNFDPKPQKTRNDSSYNLFIWG
                     RAPVALATLNDSSYNSDEPATPAQRRAS"
     gene            complement(381..1673)
                     /locus_tag="PXD94_002769"
     CDS             complement(381..1673)
                     /locus_tag="PXD94_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL6287557.1"
                     /translation="MGTYIPHREGIIQTIVRNSLTQYQAYASPSQTILDRLEHFAECA
                     DWTKGVVRIRCHDCGHSYFRPFSCKVFHLCPSCDQKRTLLYAEYLAEDLLLDLPHRQF
                     VFTIPKILRPYFKSDKRLFGEVSRLVFSLLSDFFSLAAGQELLCACVVSYQSFGEFAR
                     FHPHWHVLVLEGGFTKYDRFVYLPIGADEGMLKVWQAAIMALFLRKELIDQARVNMLR
                     DWKHSGFSIESETRLFNKADREALGQYVVRGATCAEKIQYDPASDTIIWTASPKGFYK
                     GKTETFKGFEFVDQLVAHLPPRRVQLLRRYGVYAGKVRKQWQERPNIYSLAPESWQKG
                     HPRESKIVPAIPQEKPETVQVSDAWSKLRKQSWARLLQKVYEVDPFVCPKCQGTMSVV
                     AIIEDPKELAKIINWAKQQEREQPVAVCARSPPELALV"
     gene            1836..2681
                     /gene="sul2"
                     /locus_tag="PXD94_002770"
     CDS             1836..2681
                     /gene="sul2"
                     /locus_tag="PXD94_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDL6287558.1"
                     /translation="MEYTSKSQIARNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQA
                     RKLMAEGADVIDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQ
                     AYALSRGVAYLNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHI
                     AAFFDARIAALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLL
                     SVSRKSFLRALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKE
                     TARIR"
     gene            2850..3653
                     /locus_tag="PXD94_002771"
                     /note="aph(3'')-Ib"
     CDS             2850..3653
                     /locus_tag="PXD94_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDL6287559.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3653..4489
                     /locus_tag="PXD94_002772"
     CDS             3653..4489
                     /locus_tag="PXD94_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDL6287560.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT         1099 a         1265 c         1279 g         1175 t
ORIGIN      
        1 atcgacgctc gccgttgtgc tggtgttgcc ggttcatcag aattatagga actgtcattt
       61 aaagtcgcta aggctacagg tgcacgcccc caaataaata aattatagga actgtcattt
      121 cgggtttttt gcggtttcgg gtcaaaattc ggttttttcc ccgaactacg actgttttcg
      181 caccccgaac aactaatcca atcagaattt tccggcaacg ccgactccaa gaagtgtaat
      241 tccagtctat ttctgccgtc aacccctgat tcgcgttaat gcgacaaagc tcaaacacat
      301 aactccccca cgaagccata taaactccga tttcgaggtg ttatcctgtt ccgcataaac
      361 ttcgtttttg ggtattatac ctacaccaaa gcaagctcag gaggtgaacg agcacagacg
      421 gccactggct gctcccgttc ctgctgcttt gcccagttaa taatcttggc aagctccttg
      481 ggatcctcga ttatcgccac aaccgacatt gttccctggc attttgggca aacaaacggg
      541 tcaacttcat agactttctg taacaaccgt gcccaacttt gtttgcgcaa cttggaccag
      601 gcatcggaaa cttggacagt ttccggtttc tcctgaggta tggctggaac aattttggat
      661 tcgcgtggat gacctttttg ccaactttct ggggccaggc tataaatatt ggggcgctct
      721 tgccactgct tgcggacttt gcccgcatat acgccatagc gacgaagcaa ttgaacccgc
      781 cttggtggta aatgggccac aagctggtcg acgaattcaa agcctttgaa ggtctctgtt
      841 tttcctttgt agaatccctt tgggctagct gtccagatta tcgtgtcgga agctggatca
      901 tactgaattt tttctgcaca ggtagcgccg cgaacgacat actggccgag ggcttctcga
      961 tcggccttgt tgaatagtct ggtttcgctc tcgatgctaa agccggaatg cttccagtct
     1021 ctgagcatgt tcactcgggc ttgatctatt agttcctttc gcagaaacaa agccataatc
     1081 gctgcttgcc agaccttgag catcccttcg tcggctccaa ttggtaaata tacaaagcgg
     1141 tcgtacttgg tgaacccacc ttccagaacc agaacatgcc agtgcgggtg gaatcgagcg
     1201 aattcaccaa acgactgata gctgacaaca caggcacata gtagttcttg accggctgcc
     1261 agggagaaaa aatcagaaag caaggagaat accagcctgg acacctcgcc aaacagtcgc
     1321 ttatccgatt taaagtatgg tctgagaatc ttgggaatcg taaaaacaaa ttgccgatgc
     1381 ggtagatcaa ggagcaaatc ctcggccagg tattcagcat agagtaaggt ccgtttttgg
     1441 tcacaggatg ggcaaaggtg gaaaaccttg cacgaaaatg gcctgaagta cgaatgtcca
     1501 caatcatggc aacgtattcg aacaacaccc ttggtccaat cggcacattc agcaaaatgc
     1561 tcaaggcggt caaggatagt ttgcgatgga cttgcgtagg cttggtactg ggttagagaa
     1621 ttgcggacta tggtctggat tatcccttcc cgatggggaa tgtaagttcc catacctata
     1681 catacgcagg aaaacgatca tttgcttgca attgcgaaga aaataatgga aaatgtggac
     1741 tggcgccacc taacaaccgg ttcgagcgga ctcacgggac aagcccgttc gcagctcaac
     1801 cgaaagttag gttgacgcct tcggcgcgga ggaaaatgga atatacaagt aaatcacaaa
     1861 tagctcgaaa taaatcgctc atcattttcg gcatcgtcaa cataacctcg gacagtttct
     1921 ccgatggagg ccggtatctg gcgccagacg cagccattgc gcaggcgcgt aagctgatgg
     1981 ccgagggggc agatgtgatc gacctcggtc cggcatccag caatcccgac gccgcgcctg
     2041 tttcgtccga cacagaaatc gcgcgtatcg cgccggtgct ggacgcgctc aaggcagatg
     2101 gcattcccgt ctcgctcgac agttatcaac ccgcgacgca agcctatgcc ttgtcgcgtg
     2161 gtgtggccta tctcaatgat atccgcggtt ttccagacgc tgcgttctat ccgcaattgg
     2221 cgaaatcatc tgccaaactc gtcgttatgc attcggtgca agacgggcag gcagatcggc
     2281 gcgaggcacc cgctggcgac atcatggatc acattgcggc gttctttgac gcgcgcatcg
     2341 cggcgctgac gggtgccggt atcaaacgca accgccttgt ccttgatccc ggcatggggt
     2401 tttttctggg ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc gatgaattgc
     2461 ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt ctgcgcgcgc
     2521 tcacaggccg tggtccgggg gatgtcgggg ccgcgacact cgctgcagag cttgccgccg
     2581 ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc gacgggctgg
     2641 cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt cgggatattt
     2701 ctctatattc gcggttcagc aggcatgtcc cctttgaggg cgacccgacg acaggataat
     2761 cgaccttatg gtgcgcaaat atttcggcac agatggtatt cgtggcaaag ccacctccgc
     2821 gcttcatcag aaaactgaag gaacctccat tgaatcgaac taatattttt tttggtgaat
     2881 cgcattctga ctggttgcct gtcagaggcg gagaatccgg tgattttgtt tttcgacgtg
     2941 gtgacgggca tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag ctcgctggag
     3001 agcgtgaccg cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag gtgatcaact
     3061 ggcaggagga acaggagggt gcatgcttgg tgataacggc aattccggga gtaccggcgg
     3121 ctgatctgtc tggagcggat ttgctcaaag cgtggccgtc aatggggcag caacttggcg
     3181 ctgttcacag cctatcggtt gatcaatgtc cgtttgagcg caggctgtcg cgaatgttcg
     3241 gacgcgccgt tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta ccggacgagg
     3301 acaagagtac gccgcagctc gatcttttgg ctcgtgtcga acgagagcta ccggtgcggc
     3361 tcgaccaaga gcgcaccgat atggttgttt gccatggtga tccctgcatg ccgaacttca
     3421 tggtggaccc taaaactctt caatgcacgg gtctgatcga ccttgggcgg ctcggaacag
     3481 cagatcgcta tgccgatttg gcactcatga ttgctaacgc cgaagagaac tgggcagcgc
     3541 cagatgaagc agagcgcgcc ttcgctgtcc tattcaatgt attggggatc gaagcccccg
     3601 accgcgaacg ccttgccttc tatctgcgat tggaccctct gacttggggt tgatgttcat
     3661 gccgcctgtt tttcctgctc attggcacgt ttcgcaacct gttctcattg cggacacctt
     3721 ttccagcctc gtttggaaag tttcattgcc agacgggact cctgcaatcg tcaagggatt
     3781 gaaacctata gaagacattg ctgatgaact gcgcggggcc gactatctgg tatggcgcaa
     3841 tgggagggga gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc tcgaatatgc
     3901 cggggagcga atgctctctc acatcgttgc cgagcacggc gactaccagg cgaccgaaat
     3961 tgcagcggaa ctgatggcga agctgtatgc cgcatctgag gaacccctgc cttctgccct
     4021 tctcccgatc cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg atcaaaacgc
     4081 aggttgtcaa actgactacg tccacgcggc gattatagcc gatcaaatga tgagcaatgc
     4141 ctcggaactg cgtgggctac atggcgatct gcatcatgaa aacatcatgt tctccagtcg
     4201 cggctggctg gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg gcgccgccaa
     4261 tatgttctac gatccggctg acagagacga cctttgtctc gatcctagac gcattgcaca
     4321 gatggcggac gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc tcgaccaggc
     4381 gtacgcttat gggtgccttt ccgcagcttg gaacgcggat ggagaagagg agcaacgcga
     4441 tctagctatc gcggccgcga tcaagcaggt gcgacagacg tcatactaga tatcaagcga
     4501 cttatcctat cccctgggaa cacatcaatc ttaccggaga atatcgttgg ccaaagcctt
     4561 agcgtaggat ttcgccctct cccgcaaacg acccctttat tgtaggtggg ccagttggtg
     4621 attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga
     4681 tccttcaact cagcaaaagt tcgatttatt caacaaatcc acgttgtgtc tcaaaatctc
     4741 ttatgttata ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     4801 ataaacagta atacaagg
//
LOCUS       DANLXQ010000357         4384 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.guided.180, whole genome shotgun sequence.
ACCESSION   DANLXQ010000357 DANLXQ010000000
VERSION     DANLXQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.guided.180"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            <1..257
                     /locus_tag="PXD94_002773"
     CDS             <1..257
                     /locus_tag="PXD94_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL6287561.1"
                     /translation="DEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIE
                     DPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            726..1172
                     /locus_tag="PXD94_002774"
                     /pseudo
     CDS             726..1172
                     /locus_tag="PXD94_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
     gene            1449..2663
                     /gene="floR"
                     /locus_tag="PXD94_002775"
     CDS             1449..2663
                     /gene="floR"
                     /locus_tag="PXD94_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HDL6287562.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSVLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNSDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            2691..2996
                     /locus_tag="PXD94_002776"
     CDS             2691..2996
                     /locus_tag="PXD94_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL6287563.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            3108..>4384
                     /locus_tag="PXD94_002777"
     CDS             3108..>4384
                     /locus_tag="PXD94_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HDL6287564.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRAS"
BASE COUNT          844 a         1268 c         1350 g          922 t
ORIGIN      
        1 ctgatgaacc ggcaacacca gcacaacggc gagcgtcgat gacatgggcg caacggctca
       61 agcgtgtttt caatatcgac atcgagacct gcagcggctg cggcggcgcc atgaaagtca
      121 tcgcctgcat tgaagaccct atagtgatca agcagatcct tgatcacctg aagcacaaag
      181 ccgaaaccag cgggaccagg gcgttacccg aaagccgggc gccaccggct gagctgctcc
      241 tgggtctgtt tgactgacga gcctgaaggc caacgatacc aatcaaaatg ctgcgttcac
      301 agcgccgcgg cagggatccg ccgtgctggt tgtcggaaaa ggagccgcta gtgggaaaga
      361 ggagggtaaa ttttcagcgt tgctggctcc ccgtcagccg gattgggttg catcgcaggg
      421 gtgtcgaaag agtcaactgc ggtccaaagc tgttggactt gggtgaaaag ggcgtttatt
      481 cttcctatac gtgccgcgtt ccaggcggcg actatgaggg ctatgtcgat gcccatgtgc
      541 gccggctgga ggcgctacgc cgggccggta tcgtcgagcg gatcgacgcc gaccaatggc
      601 gcatccccga tgatctggtc agccgtgccg ccgcccatga cgccggccga gacagtcagg
      661 ccagcgttcg cgtcctttcc ccggtcgatc tgaacaaaca gatcggatcg gacggcgcga
      721 cctggctgga ccggcggctg atccacggcg agacggccga ccttgcgcca accggcttcg
      781 ggcaacaagt ccgcgaagcc atggaccagc gccgcgagca ccatatcgaa cagggcgacg
      841 ccacccgcag ccgggacagc cgcgtcttct accggcgcaa ccttctcgcc atcctgcggg
      901 agcgcgaggt agccggcgtc ggatcggata tggctttgag taagggcctg ccgttccgcg
      961 ccgccacgga cggcgagagc gtcagcggca agtttaccgg aaccgtgcat ctatcgagcg
     1021 gcaagttcgc cgtggtcgag aaatcccatg agttcaccct tgtcccgtgg cggccgatca
     1081 tcgaccgcca actcggccgc gaggttatgg gcatcgtgca gggcgggtcg gtgtcgtggc
     1141 agttagggcg gcagaggggg ctggaacgct gagtgcgccc atgccgcatt gcgaagcaaa
     1201 agataatcgg ataaaatgta gcaattcata ttcgtaagcg tggagtaatc agatgggaaa
     1261 ttccaagtca gcagacaagt aagccgcaac aaccagtatt gttgttgcgg cgctctgtaa
     1321 ggctagtctc atctgattgc tgacgagcag acgtcgcccg gtattcctta atcgagggtt
     1381 gattcgtcat gaccaccaca cgccccgcgt gggcctaaac aaatatcaat cgcaaggaga
     1441 tcatattcat gaccaccaca cgccccgcgt gggcctatac gctgccggca gcactgctgc
     1501 tgatggctcc tttcgacatc ctcgcttcac tggcgatgga tatttatctc cctgtcgttc
     1561 cagcgatgcc cggcatcctg aacacgacgc ccgctatgat ccaactcacg ttgagcctct
     1621 atatggtgat gctcggcgtg ggccaggtga tttttggtcc gctctcagac agaatcgggc
     1681 gacggccaat tctacttgcg ggcgcaacgg ctttcgtcat tgcgtctctg ggagcagctt
     1741 ggtcttcaac tgcaccggcc tttgtcgctt tccgtctact tcaagcagtg ggcgcgtcgg
     1801 ccatgctggt ggcgacgttc gcgacggttc gcgacgttta tgccaaccgt cctgagggtg
     1861 tcgtcatcta cggccttttc agttcggtgc tggcgttcgt gcctgcgctc ggccctatcg
     1921 ccggagcatt gatcggcgag ttcttgggat ggcaggcgat attcattact ttggctatac
     1981 tggcgatgct cgcactccta aatgcgggtt tcaggtggca cgaaacccgc cctctggatc
     2041 aagtcaagac gcgccgatct gtcttgccga tcttcgcgag tccggctttt tgggtttaca
     2101 ctgtcggctt tagcgccggt atgggcacct tcttcgtctt cttctcgacg gctccccgtg
     2161 tgctcatagg ccaagcggaa tattccgaga tcggattcag ctttgccttc gccactgtcg
     2221 cgcttgtaat gatcgtgaca acccgtttcg cgaagtcctt tgtcgccaga tggggcatcg
     2281 caggatgcgt ggcgcgtggg atggcgttgc ttgtttgcgg agcggtcctg ttggggatcg
     2341 gcgaacttta cggctcgccg tcattcctca ccttcatcct accgatgtgg gttgtcgcgg
     2401 tcggtattgt cttcacggtg tccgttaccg cgaacggcgc tttggcagag ttcgacgaca
     2461 tcgcgggatc agcggtcgcg ttctacttct gcgttcaaag cctgatagtc agcattgtcg
     2521 ggacattggc ggtggcactt ttaaacagtg acacagcgtg gcccgtgatc tgttacgcca
     2581 cggcgatggc ggtactggtt tcgttggggc tggtgctcct tcggctccgt ggggctgcca
     2641 ccgagaagtc gccagtcgtc taaccgacga ctggtagcag gcccgctccg atgcggcgca
     2701 ctaaccatcg aaacctcgtg aatgtcggta tcctgtctgg caggataccg ctcatttccc
     2761 ttgttcagtt catcgccgtc gccgagcatc tgaattttcg gcatgcggcc aaggcacttg
     2821 gtatcagcca gtcgagcgtc agcgcgcgtg tgaaagcgct ggaggataac cttggtgtcc
     2881 tgctatttga gcgccatgcg cggggcgttc ggctaacaga cgcaggcagg cacttcatgg
     2941 agcgtgtcac ggcgggtgtc gatcaactcg atcacgcagt gaagaccgcg gagtgacggg
     3001 cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg
     3061 ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg
     3121 cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc
     3181 aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag
     3241 agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaatgcg
     3301 ggcggctgga gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc gagcacctgg
     3361 tcgctttcag ctgtaagcgt cgcggtttct gcccgagctg tggggcgcgg cggatggccg
     3421 aaagtgccgc cttgctggtt gatgaagtac tgcctgaaca acccatgcgt cagtgggtgt
     3481 tgagcttccc gtttcagctg cgtttcctgt ttgccagccg gcccgagatc atggggtggg
     3541 tgctgggcat cgtttaccgc gtcattgcca cgcacctggt caagaaagcg ggccataccc
     3601 accaagtggc caagacgggc gcggtcaccc tgatccagcg ttttggatcg gcgctcaatc
     3661 tgaatgttca cttccacatg ctgtttctcg acggtgtgta tgtcgagcaa tcccacggct
     3721 cagcgcgttt ccgctgggtc aaggcgccga ccagcccaga gctcacccag ctgacgcaca
     3781 ccatcgccca ccgggtgggt cgctatctgg aacggcaagg cctgctggaa cgggatgtcg
     3841 aaaacagcta tctggcctcg gatgcggtgg atgacgaccc gatgacaccc ctgctggggc
     3901 actcgatcac ttaccgtatc gctgtcggtt cacaggcggg gcgaaaggtg ttcactttgc
     3961 aaactctgcc gaccagtggt gatccgttcg gtgacgggat tggcaaggta gccgggtcca
     4021 gcctgcacgc cggcgtggcg gccagggccg atgaacgcaa gaagctcgaa cggctgtgcc
     4081 ggtacatcag ccgcccggcg gtatccgaga agcggctgtc gttaacacga ggcggcaacg
     4141 tgcgctacca gctcaagacg ccgtaccggg acggcaccac gcacgtcatt ttcgaaccat
     4201 tggatttcat tgcaaggctg gccgccctgg taccgaagcc cagagtcaac ctaacccgct
     4261 tccacggggt gttcgcaccc aacagtcggc accgggcgtt ggtcacgccg gcaaaacggg
     4321 gcaggggcaa caaggtcagg gtggctgatg aaccggcaac accagcacaa cggcgagcgt
     4381 cgat
//
LOCUS       DANLXQ010000358         3457 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.guided.207, whole genome shotgun sequence.
ACCESSION   DANLXQ010000358 DANLXQ010000000
VERSION     DANLXQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.guided.207"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            47..1249
                     /gene="tet(H)"
                     /locus_tag="PXD94_002778"
     CDS             47..1249
                     /gene="tet(H)"
                     /locus_tag="PXD94_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL6287565.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
     gene            complement(1345..2655)
                     /locus_tag="PXD94_002779"
     CDS             complement(1345..2655)
                     /locus_tag="PXD94_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL6287566.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRC
                     VITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDAT
                     VIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFS
                     GWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMG
                     NWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSFEALRAKILFTEGFQKIKKPR
                     YQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVREIEAGRL"
     gene            complement(2655..2957)
                     /locus_tag="PXD94_002780"
     CDS             complement(2655..2957)
                     /locus_tag="PXD94_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287567.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            complement(2998..3186)
                     /locus_tag="PXD94_002781"
     CDS             complement(2998..3186)
                     /locus_tag="PXD94_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287568.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
BASE COUNT          819 a          818 c          814 g         1006 t
ORIGIN      
        1 agatatatta cacccactct atcactgata gatttttagg atctcaatga ataaatcaat
       61 tattattata ctgctgatca ccgtattaga tgccattggt atcgggctta tcatgccagt
      121 actccctact ctattaaatg aatttgtcag tgaaaattca ctggcaaccc attacggtgt
      181 gctattagcg ctctatgcta ccatgcaggt tatttttgct cctattctag gacgactgtc
      241 tgataaatac ggcagaaaac ccatcttgct gttttccctt ttaggcgcgg cactcgacta
      301 tcttttaatg gcattctcaa ccacactttg gatgctctat attgggcgca tcattgcggg
      361 gatcacaggc gcaacaggtg ccgtatgtgc atcagcgatg agtgatgtga ctcccgctaa
      421 aaatcgaact cgctattttg gtttcttagg tggtgctttt ggtgttggcc ttattatcgg
      481 cccaatgcta gggggattat taggtgatat cagtgctcat atgccattta tttttgccgc
      541 tatttcacac tcgatattat taatactctc tttgctcttt ttccgagaaa cacaaaaaag
      601 agaagcgctt gttgccaata ggacacctga aaaccaaact gcctcaaata cagtcactgt
      661 tttttttaag aaaagcctct acttttggtt agcaacctat tttattatcc agcttatcgg
      721 gcaaattcct gccaccatct gggtgctgtt tacacaatat cgttttgatt ggaacacaac
      781 ttctatcggt atgtctttgg cggttctggg tgtattaaat attttctttc aggcgattgt
      841 cgctgggaaa ttggcacaaa aatggggcga aaaaaccacc attatgatca gtatgtctat
      901 tgatatgatg ggctgtttat tattagcgtg gataggccac gtttgggtca tcttaccagc
      961 attaatttgc ttagcggcag gaggtatggg gcaacccgca ttacaaggtt atttatcaaa
     1021 atctgtcgat gataatgcgc aagggaaatt acaaggtact ctggtgagcc taaccaatat
     1081 taccgggatc attggtcccc ttttatttgc ctttatttat agttatagcg tcgcttattg
     1141 ggatggtctg ttatggctga tgggggcaat actttatgct atgttgctta ttaccgctta
     1201 ttttcaccaa agaaaaacca cacctaaagc tgttatttca accccttaat actagaaatt
     1261 ataaagatca taaaagtcct gtccgctctt aataacgttg ataggacttt atttgggtat
     1321 ccggattaca gggttgaaac agggctataa acgccccgcc tcaatctccc gcaccagtgt
     1381 tgatatgtca gcgccgtagt tagtggatgg ccccgcttcg gcaacgccat agaacggcat
     1441 cctgcccata gcgccctcgg gtatccgttg acgctgatag cgtggcttct tgatcttctg
     1501 gaagccctca gtaaacagga tcttggctcg cagcgcttca aagctgtagc cacggccaac
     1561 acgattaaca acacgaatca ggttgttcag tgactcggtg taggcgttgg taatggggtg
     1621 gtcgaagtaa gcgaaaatgt catcgtgcca gttgcccatc gctttgataa gcgggtcata
     1681 gtgggctttc atttccggcg taatctgtcg cagccaagag taatatgcct cctgtgcctg
     1741 gtgccgggtc tgacagtccc aaatctcgaa aaaggcttct ttcagttcgt aggcttggcc
     1801 aatctcaggg taattcaatg tccagcctga aaaacgcatg tattcggcat ctgtcagctc
     1861 gtgacggcgt ttcagtagga caaacctatc tcgcatcaac ccacggcgct gctgtggcgt
     1921 cagggcgctt ctgatggctt ttctggcgcg ttccagtgat tcgttcgcca tcctgacaac
     1981 gtggaacttg tcgatgatca ccgtggcgtc aggaatcatg gtttctactg cttgccggta
     2041 cggcctccac atatccattg ccacgtagcg caccaggtcg cggtcagtgc gtttgctcag
     2101 gtagcgggtg acggtggttt tattgcggtt atccagcatg tccacaatgg tctgctgctg
     2161 aatgttggtg atcacgcagc ggggtttgat gatgtggatt tcgtcgatac ccaaccactg
     2221 cggcatttca aaattgagcg tcttttcgag gcgttcacaa tagtcgttga aaatgttacg
     2281 gacggtcttt tcatcgacgc ccacatcctc ggcaacactg gagaacgtgc ggcgcaggct
     2341 ctcgcgctcg atgtactgga tcaggcggtt ggtcatctgc cgcttttcgt ctttgtgcgg
     2401 tacaggctcc agaaacgtct ttcggcaaga ctggcagcga tagcgacggc ggttaagcat
     2461 gatgccggta cgccttccgt ggacgggtgt atccatgatc acctcatcac ggcgaccaaa
     2521 gccgacgatt tcaggatgat tgcagtgggg gcactgcgta ggcggttcgg aaacttcggc
     2581 gtgtacctgg taatcgtgct ccagttcgga tacctggagc actttgtaat caggccagtt
     2641 gaggatattc gacattactc ctttgccacc ttaaccgatt cctcggtgcc agtggcagca
     2701 gtgactagcg cgttatgaac tcgacgactg gtatcttcac tgtctgtttc aatccacgcg
     2761 ccaaaggcat gcaccattgc agcaaaagac ttcccatcaa ggatttcacc agacttgcct
     2821 ctgagcaaag cgcccatacc atagctgctt gacggatgat cggcagtcca cctgagagtc
     2881 tcgggagaca tttggcgaag ccccattcgc tttatcgtgg cgatagactc gtccggaaaa
     2941 tagagtttga ttttcatgct gacctcgctg aaagccgccc ctagggcggc atgttagtca
     3001 aattgcttcg ccgcggcgga tgttgcgaat acgaaaacgg aggtgctttg tcgcttggtg
     3061 aaccccttgg ccgcgtaatt ctgattcttt attcgggttg ttgttgatta ggtcttcagc
     3121 aaccctttca agctcgctaa gcgcctcttc gatttcattc agcgcatcat ttgcatcgta
     3181 tgacatggct tctatcctgc accaagttcg gcggtgcgcc cgttgggctt atgcccgagc
     3241 cgttgttctc agctcttgaa tctaatatta gggcatctgg ccttttccgt caaccctaaa
     3301 atccggttta tttacaaaat tacgtctagc gcagatcaac cgctaaatcc ggatacccaa
     3361 aatatcatat ataaatacta tcaaattaaa gatgttgtgt cgaaattaat gtaaaaaaag
     3421 agaacccttt taaaataaag ggttctctat atttata
//
LOCUS       DANLXQ010000359         2615 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.guided.234, whole genome shotgun sequence.
ACCESSION   DANLXQ010000359 DANLXQ010000000
VERSION     DANLXQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.guided.234"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            328..465
                     /locus_tag="PXD94_002782"
     CDS             328..465
                     /locus_tag="PXD94_002782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL6287569.1"
                     /translation="MKPLRIGARVIYPLQDLDKQIAYLEANLLENIKVHFIVESIEIK
                     G"
     gene            600..1517
                     /locus_tag="PXD94_002783"
     CDS             600..1517
                     /locus_tag="PXD94_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class A beta-lactamase
                     ROB-1"
                     /protein_id="HDL6287570.1"
                     /translation="MLNKLKIGTLLLLTLTACSPNSVHSVTSNPQPASAPVQQSATQA
                     TFQQTLANLEQQYQARIGVYVWDTETGHSLSYRADERFAYASTFKALLAGAVLQSLPE
                     KDLNRTISYSQKDLVSYSPETQKYVGKGMTIAQLCEAAVRFSDNSATNLLLKELGGVE
                     QYQRILRQLGDNVTHTNRLEPDLNQAKPNDIRDTSTPKQMAMNLNAYLLGNTLTESQK
                     TILWNWLDNNATGNPLIRAATPTSWKVYDKSGAGKYGVRNDIAVVRIPNRKPIVMAIM
                     STQFTEEAKFNNKLVEDAAKQVFHTLQLN"
     gene            1528..2445
                     /locus_tag="PXD94_002784"
     CDS             1528..2445
                     /locus_tag="PXD94_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL6287571.1"
                     /translation="MKKKLLWILILGLIIISCKQRKTEMKEKIIKTNGIELCTESFGN
                     KKNPAILLVAGATVSMLYWDTEFCQQLSEKGFFVIRYDNRDVGKSTNYEPGSTPYDIV
                     DLTNDAISILDGYKIDKAHFVGISLGGLISQIASIKFADRVNSLTLMSSGPWGDSDPT
                     IPEMDTSILDFHSKAGTVNWTNEDSVVNYLIQGAELMSGKKQFDKQRSEKLIRAEFNR
                     ANNYISMFNHAALQGGGEEYWNRLNEIKQPTLIIHGTDDKIWHYKNAGFLLEKIKGSN
                     LITLEGTGHELHVDDWKSIIDGIEKHIND"
BASE COUNT          890 a          488 c          517 g          720 t
ORIGIN      
        1 tccctttttc ttttctaaag cattggtatt aaaggcttta aggtgtattt tattaaaatc
       61 tgtacacctt tgtgaaaatc catacacctt tcttaccgtg taattttatg aaaattcgtg
      121 cacctttctg ctaacgccct gcggttgtta gactaaaaaa gccattgaaa gcaagtttca
      181 acggcttttt cagtcccgaa taaaacaaaa ccgccttgtt aggcggttat gtgttaaaat
      241 cactttcgca ctatggcggc ggctcgcact accgttcata gtgtgagcct agtcgcttag
      301 ccttatagtg ctaagggggt gatttatgtg aaacctctac gaattggggc aagggttatt
      361 tatccgcttc aagacttaga caagcaaatt gcatacctag aggcgaactt acttgagaat
      421 atcaaagtgc atttcattgt agaaagcatt gagattaaag gctaaattgc tagacttttg
      481 cgcttaaatt cgccaaaatc tgtttttttg ttctacaaac aaattatccg aatttccgcg
      541 caatgtacgt ttcaggctgc ctgaaaagaa atttatttag taaaatcaaa ggataattta
      601 tgttaaataa gttaaaaatc ggcacattat tattgctgac attaacggct tgttcgccca
      661 attctgttca ttcggtaacg tctaatccgc agcctgctag tgcgcctgtg caacaatcag
      721 ccacacaagc cacctttcaa cagactttgg cgaatttgga acagcagtat caagcccgaa
      781 ttggcgttta tgtatgggat acagaaacgg gacattcttt gtcttatcgt gcagatgaac
      841 gctttgctta tgcgtccact ttcaaggcgt tgttggctgg ggcggtgttg caatcgctgc
      901 ctgaaaaaga tttaaatcgt accatttcat atagccaaaa agatttggtt agttattctc
      961 ccgaaaccca aaaatacgtt ggcaaaggca tgacgattgc ccaattatgt gaagcagccg
     1021 tgcggtttag cgacaacagc gcgaccaatt tgctgctcaa agaattgggt ggcgtggaac
     1081 aatatcaacg tattttgcga caattaggcg ataacgtaac ccataccaat cggctagaac
     1141 ccgatttaaa tcaagccaaa cccaacgata ttcgtgatac gagtacaccc aaacaaatgg
     1201 cgatgaattt aaatgcgtat ttattgggca acacattaac cgaatcgcaa aaaacgattt
     1261 tgtggaattg gttggacaat aacgcaacag gcaatccatt gattcgcgct gctacgccaa
     1321 catcgtggaa agtgtacgat aaaagcgggg cgggtaaata tggtgtacgc aatgatattg
     1381 cggtggttcg cataccaaat cgcaaaccga ttgtgatggc aatcatgagt acgcaattta
     1441 ccgaagaagc caaattcaac aataaattag tagaagatgc agcaaagcaa gtatttcata
     1501 ctttacagct caactaacaa attcagcatg aaaaaaaaac tactttggat attaatttta
     1561 ggactgataa taatcagttg caaacaaagg aaaacagaaa tgaaagagaa aataattaaa
     1621 acaaacggca ttgaactctg tacggaaagt tttggaaata agaaaaatcc agcaatcctt
     1681 ttggtagcag gtgcaaccgt atcaatgctg tattgggaca ctgaattttg ccaacaatta
     1741 tctgaaaaag gattttttgt tattcgttac gacaacagag atgtaggaaa atccactaat
     1801 tatgaaccag gttctactcc atacgatatt gttgacttaa ctaatgacgc tatttcaata
     1861 ttggatggct acaagattga caaagcacat tttgtgggga tttctttggg cggactaatt
     1921 tctcaaatag catcaataaa gtttgccgac agagttaact ccttaactct tatgtcatca
     1981 ggcccttggg gagactcaga cccaactata cctgaaatgg acacgagtat tttagatttc
     2041 catagtaaag caggtacagt caattggaca aatgaagaca gtgtggtaaa ctatttaatt
     2101 cagggtgcag aattaatgag tggcaagaaa caatttgaca aacaaagaag tgaaaaactg
     2161 ataagagctg agttcaatag agccaacaat tatataagta tgttcaatca cgctgcattg
     2221 caaggtggtg gtgaagaata ttggaacaga ttaaacgaaa tcaaacaacc caccttaatt
     2281 atccacggaa cagacgacaa aatttggcat tataagaatg caggtttttt actagaaaaa
     2341 ataaaaggtt caaatctaat cacccttgaa ggtacaggac acgaattaca cgttgatgat
     2401 tggaaatcaa taattgatgg aatagaaaaa cacataaatg actgataaac aaaaacaacg
     2461 acccgctaac aatgtatata aaaaataggc gaaacagtag taaattcaag gcttttggct
     2521 cgtattatac tttgtgctta accgaaaatt tggcacttcg aaatcgccta cttttcgctg
     2581 aatttacaat ccaagtgcaa caaaaaaaga agtac
//
LOCUS       DANLXQ010000360         2535 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.guided.236, whole genome shotgun sequence.
ACCESSION   DANLXQ010000360 DANLXQ010000000
VERSION     DANLXQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.guided.236"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            60..1535
                     /gene="msr(E)"
                     /locus_tag="PXD94_002785"
     CDS             60..1535
                     /gene="msr(E)"
                     /locus_tag="PXD94_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HDL6287572.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     gene            1591..2475
                     /locus_tag="PXD94_002786"
     CDS             1591..2475
                     /locus_tag="PXD94_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="HDL6287573.1"
                     /translation="MTIQDIQSLAEAHGLLITDKMNFNEMGIDFKVVFALDTKGQQWL
                     LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAE
                     TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV
                     KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH
                     IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL
                     ETQNESLIVGAKAQLGVI"
BASE COUNT          866 a          416 c          545 g          708 t
ORIGIN      
        1 gttataactt aattgactag ctattaaaaa tttcaagagc taaacaggag taaataaaaa
       61 tgagtttaat tattaaagcg agaaacatac gcttggatta tgctgggcgt gatgttttgg
      121 atattgatga attggaaatt cactcttatg accgtattgg tcttgtgggt gataacggag
      181 caggaaagag tagtttactc aaagtactta atggcgaaat tgttttagcc gaagcgacat
      241 tacagcgttt tggtgatttt gcacatatca gccaactggg cggaatcgaa atagaaacgg
      301 tcgaagaccg ggcaatgtta tctcgccttg gtgtttccaa tgtacaaaac gacacaatga
      361 gtggcggaga ggaaactcgt gcaaaaattg ctgccgcatt ttcccaacaa gtacatggca
      421 ttctagcgga tgaaccaacc agccaccttg atctcaatgg aatagatcta cttattggtc
      481 aacttaaagc atttgatgga gcattacttg ttatcagtca tgaccgatat tttcttgata
      541 tggttgtaga caagatatgg gagttaaaag acggtaaaat tacggaatat tggggtggtt
      601 actcggatta cttgcgtcaa aaagaagaag agcgacaaca ccaagccgta gaatatgagc
      661 tgatgatgaa ggaacgggag cgattagaat ctgctgtgca agaaaaacgc cagcaagcta
      721 atcgattaga caataagaaa aaaggagaaa aatccaaaaa ctctaccgaa agtgctggac
      781 gacttgggca tgcaaaaatg actggcacca agcaaagaaa actgtatcag gcagctaaga
      841 gtatggaaaa gcgtttggct gcattagaag atattcaagc accagagcat ttgcgttcta
      901 ttcgttttcg tcaaagttca gccctagaac tgcacaataa gttcccgatt acggcagatg
      961 gtctgagctt aaaatttggt agccgtacta tctttgatga cgctaacttt ataataccgc
     1021 ttggcgctaa agtcgctata actggatcga atggaacagg gaaaacgtcc ttgttaaaaa
     1081 tgatatcaga acgtgctgat ggattaacca tatctccaaa agctgaaatt ggctacttta
     1141 cacaaacagg atataaattt aacacgcata aatctgtgct ctcctttatg caggaagagt
     1201 gcgagtacac agttgcggaa attcgtgcag tattggcttc aatggggatc ggagcgaatg
     1261 atattcaaaa aaacttatcc gacttatcgg gaggtgaaat catcaaactg cttttatcca
     1321 aaatgctttt aggaaaatat aatattttgc ttatggatga accaggaaac tatcttgacc
     1381 taaaaagtat tgccgcatta gaaacaatga tgaagtccta tgcaggaact attatcttcg
     1441 tatctcatga caagcaattg gtcgataata ttgctgacat tatctacgag atcaaagacc
     1501 acaaaatcat caagactttt gagagagatt gttaatgata gccaatctaa tccgaacatt
     1561 aattattgaa ctctttaaag gaaattaaaa atgacaattc aagatattca atcacttgct
     1621 gaagcacacg gcttgttgat tacggacaaa atgaatttca atgaaatggg cattgatttt
     1681 aaggtcgttt ttgctcttga tacaaagggg caacaatggt tgctgcgtat tcctcgtcgt
     1741 gatggcatga gggaacaaat caagaaagaa aaacgcattt tagaattggt aaaaaaacat
     1801 ctttctgtag aggttcctga ttggagaatt tcatctacag aattagtggc ttatcccata
     1861 cttaaagata atcctgtttt aaatttggat gctgaaacct atgaaataat ttggaatatg
     1921 gacaaagata gcccgaaata cataacatct ttggcaaaaa ccttatttga aatccatagt
     1981 attcctgaaa aagaagttcg ggaaaatgat ttgaaaatta tgaaaccttc agatttaaga
     2041 cctgaaatag caaacaattt gcagttagta aaatctgaaa ttggtataag tgagcaattg
     2101 gaaacccgct acagaaaatg gttggataat gatgttctat gggcagattt cacccaattt
     2161 atacatggcg atttatatgc tgggcatgta ctagcttcaa aggatggagc tgtttcaggc
     2221 gttattgatt ggtcaacagc ccatatagat gacccagcga ttgattttgc tgggcatgta
     2281 actttgtttg gagaagaaag cctcaaaact ctaatcatcg agtatgaaaa actagggggt
     2341 aaagtttgga ataaactata tgaacagact ttagaaagag cagcggcctc tcctttgatg
     2401 tatggtttat ttgccttaga aactcaaaat gaaagcctta tcgttggagc aaaagctcag
     2461 ttgggagtta tataatttaa aaatatgatt gctgaggcac tgttgcaaat agtcggtggt
     2521 gataaactta tcatc
//
LOCUS       DANLXQ010000361         2447 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.guided.242, whole genome shotgun sequence.
ACCESSION   DANLXQ010000361 DANLXQ010000000
VERSION     DANLXQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.guided.242"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            94..627
                     /gene="aadB"
                     /locus_tag="PXD94_002787"
     CDS             94..627
                     /gene="aadB"
                     /locus_tag="PXD94_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HDL6287574.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
     gene            685..1476
                     /locus_tag="PXD94_002788"
     CDS             685..1476
                     /locus_tag="PXD94_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDL6287575.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1553..2380
                     /locus_tag="PXD94_002789"
     CDS             1553..2380
                     /locus_tag="PXD94_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034033256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-2"
                     /protein_id="HDL6287576.1"
                     /translation="MAIRIFAILFSIFSLATFAHAQEGTLERSDWRKFFSEFQAKGTI
                     VVADERQADRAMLVFDPVRSKKRYSPASTFKIPHTLFALDAGAVRDEFQIFRWDGVNR
                     GFAGHNQDQDLRSAMRNSTVWVYELFAKEIGDDKARRYLKKIDYGNADPSTSNGDYWI
                     EGSLAISAQEQIAFLRKLYRNELPFRVEHQRLVKDLMIVEAGRNWILRAKTGWEGRMG
                     WWVGWVEWPTGSVFFALNIDTPNRMDDLFKREAIVRAILRSIEALPPNPAVNSDAAR"
BASE COUNT          593 a          585 c          700 g          569 t
ORIGIN      
        1 aataaatcaa ttattattat actgctgatc accgtattag cagcaacgat gttacgcagc
       61 agggcagtcg ccctaaaaca aaggttaggc cgcatggaca caacgcaggt cacattgata
      121 cacaaaattc tagctgcggc agatgagcga aatctgccgc tctggatcgg tgggggctgg
      181 gcgatcgatg cacggctagg gcgtgtaaca cgcaagcacg atgatattga tctgacgttt
      241 cccggcgaga ggcgcggcga gctcgaggca atagttgaaa tgctcggcgg gcgcgtcatg
      301 gaggagttgg actatggatt cttagcggag atcggggatg agttacttga ctgcgaacct
      361 gcttggtggg cagacgaagc gtatgaaatc gcggaggctc cgcagggctc gtgcccagag
      421 gcggctgagg gcgtcatcgc cgggcggcca gtccgttgta acagctggga ggcgatcatc
      481 tgggattact tttactatgc cgatgaagta ccaccagtgg actggcctac aaagcacata
      541 gagtcctaca ggctcgcatg cacctcactc ggggcggaaa aggttgaggt cttgcgtgcc
      601 gctttcaggt cgcgatatgc ggcctaacaa ttcgtccaag ccgacgccgc ttcgcggcgc
      661 ggcttaactc aggtgttaga catcatgagg gaagcggtga ccatcgaaat ttcgaaccaa
      721 ctatcagagg tgctaagcgt cattgagcgc catctggaat caacgttgct ggccgtgcat
      781 ttgtacggct ccgcagtgga tggcggcctg aagccataca gcgatattga tttgttggtt
      841 actgtggccg taaagcttga tgaaacgacg cggcgagcat tgctcaatga tcttatggag
      901 gcttcggctt tccctggcga gagcgagacg ctccgcgcta tagaagtcac ccttgtcgtg
      961 catgacgaca tcatcccgtg gcgttatccg gctaagcgcg agctgcaatt tggagaatgg
     1021 cagcgcaatg acattcttgc gggtatcttc gagccagcca tgatcgacat tgatctggct
     1081 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1141 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1201 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1261 atttggtaca gcgcaataac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1321 ataaaacgcc tacctgccca gtatcagccc gtcttacttg aagctagaca ggcttatctt
     1381 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac
     1441 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
     1501 cgccgcttcg cggcgcggct taccttggcc gttgggcatt aaggaaaagt taatggcaat
     1561 ccgaatcttc gcgatacttt tctccatttt ttctcttgcc actttcgcgc atgcgcaaga
     1621 aggcacgcta gaacgttctg actggaggaa gtttttcagc gaatttcaag ccaaaggcac
     1681 gatagttgtg gcagacgaac gccaagcgga tcgtgccatg ttggtttttg atcctgtgcg
     1741 atcgaagaaa cgctactcgc ctgcatcgac attcaagata cctcatacac tttttgcact
     1801 tgatgcaggc gctgttcgtg atgagttcca gatttttcga tgggacggcg ttaacagggg
     1861 ctttgcaggc cacaatcaag accaagattt gcgatcagca atgcggaatt ctactgtttg
     1921 ggtgtatgag ctatttgcaa aggaaattgg tgatgacaaa gctcggcgct atttgaagaa
     1981 aatcgactat ggcaacgccg atccttcgac aagtaatggc gattactgga tagaaggcag
     2041 ccttgcaatc tcggcgcagg agcaaattgc atttctcagg aagctctatc gtaacgagct
     2101 gccctttcgg gtagaacatc agcgcttggt caaggatctc atgattgtgg aagccggtcg
     2161 caactggata ctgcgtgcaa agacgggctg ggaaggccgt atgggttggt gggtaggatg
     2221 ggttgagtgg ccgactggct ccgtattctt cgcactgaat attgatacgc caaacagaat
     2281 ggatgatctt ttcaagaggg aggcaatcgt gcgggcaatc cttcgctcta ttgaagcgtt
     2341 accgcccaac ccggcagtca actcggacgc tgcgcgataa aaccgcgcag cgccggttac
     2401 ttcaacgggc actgttgcaa atagtcggtg gtgataaact tatcatc
//
LOCUS       DANLXQ010000362          892 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749874-rid16723453.guided.304, whole genome shotgun sequence.
ACCESSION   DANLXQ010000362 DANLXQ010000000
VERSION     DANLXQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749874
            Sequence Read Archive: SRR17934095
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574579.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574579.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 23:42:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,731
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 40 of 69
            Pseudo Genes (incomplete)         :: 21 of 69
            Pseudo Genes (internal stop)      :: 9 of 69
            Pseudo Genes (multiple problems)  :: 1 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749874-rid16723453.guided.304"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: IA"
                     /collection_date="2020-09-05"
                     /collected_by="Iowa State University Veterinary Diagnostic
                     Laboratory, Ames, IA"
     gene            47..862
                     /locus_tag="PXD94_002790"
     CDS             47..862
                     /locus_tag="PXD94_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDL6287577.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          238 a          178 c          210 g          266 t
ORIGIN      
        1 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
       61 acgggaaacg tcttgctcga ggccgcgatt aaattccaac ctggatgctg atttatatgg
      121 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
      181 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
      241 tacagatgag atggtcagac taaactggct gacggcattt atgcctcttc cgaccatcaa
      301 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgctcc ccgggaaaac
      361 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
      421 agcgttcctg cgccggttgc attcgattcc tgttagtaat tgtcctttta acagcgatcg
      481 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
      541 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
      601 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
      661 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
      721 ataccaggat cttgccatcc tatggaactg cctcggtgaa ttttctcctt cattacagaa
      781 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
      841 gatgctcgat gagtttttct gatagttagt ctttgtagag acacaaacct ga
//
